LOCUS       DAOQMF010000001         9348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_2875_length_9348_cov_3.639083, whole genome shotgun sequence.
ACCESSION   DAOQMF010000001 DAOQMF010000000
VERSION     DAOQMF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2875_length_9348_cov_3.639083"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLV77_00005"
     CDS             <1..563
                     /locus_tag="PLV77_00005"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14262.1"
                     /translation="VTKAGTGAGTVASNPVGINCGSDCEESYGPGTMVTLIAAAKPGS
                     TFQGWSGPCSGIRACQVTMSESRNVTATFSSVQSEGFRGRIAVTPKYQVIHRGDWEYF
                     RVQIESTNGVPASNVNVCPSSEWAALWFSGCAQLGNVPGGGSKSGLIKVGLYSWASLG
                     YDRAVAFTFSSPTFGSGTKEAIVVAR"
     gene            783..2525
                     /locus_tag="PLV77_00010"
     CDS             783..2525
                     /locus_tag="PLV77_00010"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC14263.1"
                     /translation="MIASAIGILVWGVGLFSGVEQDSVDKRFSIRGPQEPNQKVTTVL
                     IDAETFDQLNEQWPFPRSLHGNAIDRLRKAGAKVIAYDVQFTEPTIPREDNRLLSAVR
                     RAGNVALATDAVYLNGTSDVFGGVETQKLYRATVGNSATRPDSDGVIRKFPTSIDGML
                     TFPVAAARLAGLDHASVPPRSWIDFAGPAGTVPAISFSDLLTGKFEPERFKDKTVVVG
                     TSEPTLQDVSATAAGGGLMPGAEIQANEIATILDGFPLRSPSDPVVILLILLMGSIIP
                     LAGWRLRPLPTALAALAIAALYLVAAQLLFNAGVVIPVVYPMTALVLGSLGTFQVMYL
                     LLAFQRQQTRDRFSRFVPESVVEQVLADGSGDLKLGGVRTEATVLFSDIRNFTTYSES
                     RPPEDVVEVLNHYLGLMTDTIMNHGGTLISYMGDGIMAVFGAPVAQPDHADRAISGAR
                     EMLNKSLPTFNEWMKQAGYGDGFRIGIGVNTGEVMLGQVGSERRMEYTTIGDTVNTAA
                     RLEGMTKGSGFEILVSETTREACTRTVSDLDEIGEREVRGRDEAIRLWGLRAVEEKTK
                     GRDKEDSLVPFRLD"
     gene            2950..4635
                     /locus_tag="PLV77_00015"
     CDS             2950..4635
                     /locus_tag="PLV77_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14264.1"
                     /translation="MEVRIANSGYRALLSATAVLILFLAWPGSEARGSGFAALTTGAT
                     HGCALRSSGEALCWGANSEGQLGDGGTEKELIPTPVEGLADATSISAGNSHTCAIRGS
                     GQVVCWGSNGNGQLGDGLSDHLTPEPVPGLNDATSISAGDSHTCAIRSSGQAVCWGYN
                     GWGQIGDGTTDDSLTPTAVPGLTDATEIAAGWSHSCAIRTGGQVVCWGGNASGQIGDG
                     TTDDQLTPTPVTGLTDAVSIAVGQGQSCAVRTSGQAVCWGDNSGGQLGDGTTDDRLTP
                     TAVIGLTDAGEITAGGSHTCAIRTTGQAVCWGTNRDGQLGDGTSSGDEDPDGIPTPVS
                     SLSNALNIAAGDSYTCAIRGIDQAVCWGKNNGRLGDGTLQDRLLPTAVSDLPPPVIGV
                     TVNLVKIKGKVTTKCPGERRFRKLIKPRQVKVGCRLDTRKGTVSLTSAKAKKSGTQAG
                     RFWGGLFRVGQTRRKTLTTMNLTGSTKCPVNAPHRRPIRRGWGKDKGGKFRIGGHGGS
                     ASSRGTHWGVVDRCDGSTKVLVKEGSVSVRDFVRKKTVTVRAGHSYVTGKRKR"
     gene            4635..5210
                     /locus_tag="PLV77_00020"
     CDS             4635..5210
                     /locus_tag="PLV77_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14265.1"
                     /translation="MDENDADGLAGIPLGEILTRISADADGLAAGSTAAISLAFAAGL
                     VESVARTYLGEGSPASLVAQAETVRDRAIDVAGRNRVDYARARAALSGKTETDDLRLR
                     EALDSTLENLGWIAGHAADLTMLAAQLVGKVDPDREPDLESAVCLAESAARVSIKLAD
                     ANLLNSPAMPLLDEIAEDLRIVVSTREKLDH"
     gene            5236..5796
                     /locus_tag="PLV77_00025"
     CDS             5236..5796
                     /locus_tag="PLV77_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC14266.1"
                     /translation="MLRAGSLSDGDLVNRCLDGDDEAWAVFVERFSSFVYAILMRGFR
                     LSTHEAEDVFQEVFIRAFDRLDTVRDADAVKSWLAQITRRLAVDRIRSGGREVVVEEL
                     PEQTDDGFYARLDEAMDVREALEAMGGNCRDILDRFFIRDQTYRTIGEELEIPPGTIA
                     SRISRCLSNLKDQLEEPETDLAEEGR"
     gene            5830..6111
                     /locus_tag="PLV77_00030"
     CDS             5830..6111
                     /locus_tag="PLV77_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14267.1"
                     /translation="MEELNEQQIAALLRELPAPPQHLVERAKMIPRVNRQMEQVLPKF
                     ETLEKGRRVETAELEKAIEAAGLEHDKVLIDALKERLEQETDDQDGGTP"
     gene            7501..7677
                     /locus_tag="PLV77_00035"
     CDS             7501..7677
                     /locus_tag="PLV77_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14268.1"
                     /translation="MNWNNNSPVLHFGQKNRIGPFRCVSRHRGITCIKVTGRGAGKGF
                     RINRKLAVKVRISV"
     gene            complement(7740..8249)
                     /locus_tag="PLV77_00040"
     CDS             complement(7740..8249)
                     /locus_tag="PLV77_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14269.1"
                     /translation="MNFQSRIVIAFIVAIGAAVALSACSGSQPERVSGSSGGDAPTVK
                     GEFNGSDSTPSITSSDYDYSGYDDSTYDSGYDSSYGDSGYDDASYDSSYDDSYSGSSC
                     GSNLSYKLSTTTPGFAQNVADAYCDEIGSGSGYVTAYSPTMNQDYEMYCSGSPHVCTG
                     GVDAEVYFD"
     gene            complement(8395..8715)
                     /locus_tag="PLV77_00045"
     CDS             complement(8395..8715)
                     /locus_tag="PLV77_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14270.1"
                     /translation="MSEEATEQKIPKGSYAAGRRVTIPEGAEQPIVVFINGVAQSEDQ
                     DYVLRGNEILFTREIIKEEKSGKKKLVMLLGVVGFYNKDETIDVQYQLNGKTELAGDL
                     SVSK"
     gene            complement(8777..9085)
                     /locus_tag="PLV77_00050"
     CDS             complement(8777..9085)
                     /locus_tag="PLV77_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HNC14271.1"
                     /translation="MKLSTNSKTVRRGKKATFKISVHNVGNAWARNVRVCASAPKNLV
                     QIPKCVSLGSVAADNFKTASLQITVKKNANKGKRATVTFTASGQGMTSKSARASVKVQ
                     "
BASE COUNT         1809 a         2634 c         3011 g         1894 t
ORIGIN      
        1 cggtgacgaa ggcgggtacc ggcgcgggca cggtcgcctc gaatccggtt gggatcaatt
       61 gcggctcgga ttgcgaagag tcctacgggc cgggcaccat ggttacgttg atcgcggcgg
      121 ccaagccggg ttccacgttt cagggttggt ctggtccttg ctcgggcatt cgtgcctgcc
      181 aggtaactat gagtgagtcg cggaatgtga cggctacgtt ttccagcgtt caatcggaag
      241 gatttcgtgg aaggattgcc gtgaccccga aataccaggt gattcatcgc ggcgactggg
      301 aatatttccg ggtgcagata gagagcacga acggagtgcc ggcctcgaac gtgaacgtgt
      361 gtccctcctc cgagtgggca gcactctggt tttcgggctg cgcgcagctc gggaatgttc
      421 cgggtggtgg ctcgaagtcc gggctgatca aggtcgggct ttatagctgg gcttctctgg
      481 gatacgaccg agccgtggcc ttcacgttca gcagtcctac ctttggtagc gggaccaagg
      541 aagccattgt tgttgccagg tagtcgtgaa cccgggattg tcaaattcga attccgaaag
      601 ggaggattcg ggacccggtc ggcgaccggg tcccggcgtt gcgggcgtgg gtattcgctg
      661 ctaacttgac ttgacgaggc aatgacgcct caggagggag ctgtcctggt gaagaccagt
      721 cttgagaagt cggtcctggg gagcggatga accgacgacg gtccgcgctc atcgtcgttg
      781 ccgtgattgc ttcggcaatc gggatcctgg tctggggggt ggggctgttc agcggggttg
      841 aacaggactc ggtcgacaag cgattctcga ttcgaggacc gcaggaaccc aaccagaagg
      901 tgacgacggt gctgatcgat gcggaaacct ttgatcagct gaacgaacaa tggccgtttc
      961 ccaggtcact ccacggcaac gcgattgatc gtctgcgcaa agcaggggcg aaagtcatcg
     1021 catatgacgt tcagttcacc gaaccaacga tcccgcggga ggacaaccgg ttactttcgg
     1081 cggtgcgacg cgccggtaac gtggcactgg ccaccgatgc tgtgtacttg aacgggacca
     1141 gcgacgtctt cgggggagtt gagacacaga agctgtaccg ggcaaccgtt gggaattcag
     1201 ccacccgccc cgacagcgac ggagtaatac gaaagttccc cacctcgatc gatggaatgt
     1261 tgacgtttcc ggtggccgcc gcgaggttgg ctgggctgga tcacgcttcg gttccacccc
     1321 gttcctggat tgactttgcc ggaccagccg gaacggttcc ggccatttcc ttttcggatt
     1381 tgttgacggg taagttcgaa cccgagcgat tcaaagacaa gacagttgtg gtgggtactt
     1441 cggaacccac ccttcaggac gtcagcgcga ctgcagccgg gggcgggttg atgccagggg
     1501 ccgaaatcca ggcgaacgag atcgcgacga tcctggacgg tttccccttg cgatcgcctt
     1561 cagatccggt cgtgattctc ctcatcctgt tgatggggtc gattattccg ctggcaggtt
     1621 ggaggcttcg tcctcttccc accgcgctgg ctgcccttgc cattgccgcc ctgtatctgg
     1681 tcgcagcaca actcctcttc aacgccggcg tcgtgatccc ggttgtgtat ccgatgaccg
     1741 ctctcgttct gggatcgctg ggaaccttcc aggtcatgta tctgctgctg gcgtttcagc
     1801 gccagcaaac ccgagacaga ttttccaggt tcgttccgga gtccgtcgtc gagcaggtgc
     1861 tcgccgatgg ctcgggcgac ctcaaactgg gcggtgtgcg gaccgaggca acggtcctgt
     1921 tcagtgatat ccgtaacttc accacgtatt ccgagagtcg gcccccggag gatgtcgtcg
     1981 aggttctgaa tcactacctg gggctgatga ccgacacgat catgaatcac ggaggcaccc
     2041 tcatctcgta catgggggac ggcatcatgg ccgttttcgg agctccggtg gcacagcccg
     2101 atcatgcgga ccgagcgatt tccggagctc gcgaaatgct caacaagagc ctgcccacct
     2161 ttaacgaatg gatgaagcaa gctggctacg gtgacggatt tcgcatcggg attggcgtga
     2221 acaccggcga ggtgatgtta ggccaggttg gttccgagcg ccggatggaa tacacgacga
     2281 tcggcgacac tgtgaatacc gcagcccggc tcgaaggtat gaccaaagga tcgggattcg
     2341 agatcctcgt atccgaaaca accagagagg cgtgcacgcg tacggtgtca gacctggacg
     2401 aaatcggaga gcgggaagtg aggggacggg acgaagcaat caggctctgg ggactaaggg
     2461 ccgtggaaga gaagacaaag ggtcgggaca aggaagattc cctggttccc tttcgtctgg
     2521 actgacagaa gtgatgaatc tggccatctg caacaaggag caggcatcat gcgaagaacc
     2581 atcatcggga tagccgcaat cgtcgcggca cttttctttt ccggactgcc aacgagcccg
     2641 gcttcggcgg ggatgaccaa ctgcggtcgg attcgggtca acggcattgc ttacaaagtg
     2701 accatttacc ggggtcacgt ttcgtgccac agggctcgcc ggctgatcaa gacctacggt
     2761 gaaggcggtg gcaggaacgt gcgtccaccg gggaccggtc gaagtgggtg gtattcaatc
     2821 ctgccaggtc gctggcgttg ctcaagcggt gccggtggcg cacttggttg ttcacacgga
     2881 cccaaggtaa atcgttacga gtggcggata atggtcttcg gaactcagct gcccttcgcc
     2941 taggatcgga tggaggttcg gatcgcaaat tcgggttaca gggctctcct ctccgcgacg
     3001 gcggttctga ttctctttct ggcctggcca ggatcagaag caagggggtc cggattcgcg
     3061 gccctgacca ccggcgctac tcacggttgt gctctgcgaa gttccggtga ggctctgtgc
     3121 tggggtgcca attcggaagg ccagctcggt gatggtggca ccgaaaagga gctcattccg
     3181 actcccgtcg aagggctggc cgatgcgact tcgatcagtg ccggcaattc acatacatgc
     3241 gcgatccggg gttccggtca ggtggtttgc tggggcagca acggcaacgg ccagctcggt
     3301 gatggtttgt ccgaccatct caccccggaa cccgtgccgg ggctgaatga cgcgacctct
     3361 atctcggccg gcgattcgca cacctgcgcg attcgaagct cgggccaagc ggtttgctgg
     3421 ggctacaacg gatggggcca gatcggcgac ggaaccaccg atgacagtct cactccgacc
     3481 gcggtcccgg gcttgaccga tgccaccgaa atcgcagccg gctggtctca cagctgcgcg
     3541 ataaggaccg gcgggcaagt cgtctgctgg ggcggcaatg catccggcca gatcggtgat
     3601 ggcaccactg acgaccagtt gaccccgacc ccggtgaccg gcctgaccga cgcggtgtcg
     3661 atcgccgtcg gccagggaca aagctgcgct gttcgaacct cggggcaggc cgtctgctgg
     3721 ggcgacaact caggcgggca gcttggcgac ggcactactg acgaccgcct caccccgacg
     3781 gccgtgattg gcctcaccga cgccggcgaa attaccgccg gtggttcaca cacatgtgcg
     3841 atacgaacca ctggtcaggc cgtctgctgg ggtaccaatc gggatggaca actgggtgac
     3901 ggtacctcca gcggtgacga ggatcccgac ggtatcccaa cgcccgtcag ctccctgtcg
     3961 aacgcgttga acatcgccgc gggagactca tacacctgcg caatcagggg catcgatcaa
     4021 gccgtctgct ggggcaagaa caacggccgg ctcggtgacg gcacgctcca ggatcgtctt
     4081 ttgccgacgg ccgtatcgga tctgccgcct ccggtcatcg gcgtgactgt caacctcgtg
     4141 aagatcaaag gcaaagtcac caccaagtgc cctggcgagc gccgattcag gaaattgatc
     4201 aagccccgtc aggtcaaagt gggctgtcgg ctcgataccc ggaagggaac ggtgagcctc
     4261 acttccgcga aggcaaagaa gtcgggcacc caggccggca ggttctgggg cggactgttc
     4321 agggttggcc agacgcgaag gaagacactc accacgatga atttgacggg gtcgacgaag
     4381 tgtcctgtga atgctcccca tcgtcgtccg atccggaggg gttggggcaa ggacaagggg
     4441 ggcaaattcc ggatcggtgg ccacggtgga tcggcgagtt cgcgaggtac tcactggggc
     4501 gtggtggatc gttgcgatgg ttcgaccaaa gtgctggtga aggagggttc ggtcagcgtc
     4561 agggacttcg tccgcaagaa gacggtaact gtcagggccg gccattccta cgtcaccgga
     4621 aagaggaagc gttgatggat gagaatgacg ccgacggtct ggccggtatt cctcttggag
     4681 aaattctcac tcgaatctcg gcggacgccg acggtctggc ggccggctcc acagcagcga
     4741 tctctctggc cttcgcggcg ggcttggtcg aatctgtcgc tcgcacatat ctgggggaag
     4801 gttcgcccgc gtcactggtc gcccaagccg aaacggtcag ggatcgcgcg atcgacgtgg
     4861 cgggccgcaa ccgggttgat tacgctcggg ccagggcggc tttgtccggt aaaacggaga
     4921 ccgatgatct gcggctgcgt gaggcgcttg actccactct ggagaatctg ggatggatcg
     4981 cgggccatgc cgccgacctg accatgctcg ccgcacaact cgtcggcaag gtcgaccccg
     5041 atcgcgagcc ggatctggag tctgccgtgt gcctggcgga gtcggccgcc cgggtctcga
     5101 taaagctggc cgatgcaaat cttctgaatt caccggcaat gcccttgctg gacgagatcg
     5161 ccgaagacct gagaatcgtt gtttcgacgc gggaaaaact ggatcactga ccgatttcgc
     5221 tgctaggctc gccctatgct cagggctgga agcctcagcg atggggattt ggtcaatcgt
     5281 tgtctggatg gggatgatga ggcctgggct gtcttcgtcg agcgcttttc ttcgttcgtg
     5341 tacgcgatcc tcatgcgcgg cttccggctg tctacccacg aggccgagga cgtctttcag
     5401 gaagtgttca ttcgtgcttt cgatcggctc gacacggtcc gcgacgcgga cgcagtcaag
     5461 tcctggctgg cccagatcac ccgccggctg gcggtggatc gaatccgctc cggtggtcgt
     5521 gaagtggtag tcgaggagtt gccggaacag accgacgatg ggttctacgc gcgtctcgac
     5581 gaggccatgg atgttcgaga ggcactggag gcgatgggag ggaactgtcg ggatatcctc
     5641 gaccggttct tcattcggga ccagacctac agaaccatcg gcgaggagtt ggagatcccg
     5701 cccggcacca tcgccagccg gatttcccgt tgcctctcca atctgaagga tcaactggag
     5761 gagccggaaa ccgatttggc cgaggaggga agatagacgg caccagttcc gtcttgactc
     5821 tgaccgacca tggaagaact gaacgaacaa cagatcgcgg cactcctacg ggaactgcct
     5881 gccccacccc aacacttggt cgagcgggcc aaaatgatcc ctcgggtcaa tcggcagatg
     5941 gagcaggtgc tgccaaagtt tgagacgctg gaaaaaggac gccgggtcga aaccgccgaa
     6001 ctggaaaagg caatcgaggc ggccggcctc gaacacgaca aagtcctgat cgacgctctc
     6061 aaggagagac tcgaacagga gacagacgac caggatggag gcacaccatg aaagtaccgc
     6121 ggcaatgact gaaccaagac agtggaactc atccttgtcc gcagtggcgc ttgtcgcgct
     6181 ggcggtcctg cttgctctcg ggttcggaag cttcgcgggc cggtcgcagg ctgacgcttc
     6241 cgcctcctcg ccggtcgggg cccgggcaat cggtgatcgg tcgggttcgg ccgtgcatcg
     6301 caggacttct cacgtcttgc tctggaaggc acttgacggg aaagtgattt gcggagttgt
     6361 atttcacttg cagaatcaac cggccagcca gatcttgtgt caggcgcggg tgatcccgcc
     6421 acccgcaaac ccgcctccgg acggagatcc cggctttgtt ttcctcagcg gcaacggccg
     6481 acctgacagg gcaatgatca gcccgtacac ctggcaggcg ccgccacggt ctccaaagtt
     6541 cttgccaacc ctcagggtcg gtcagaaatg gtcatatcgg aggatcgggg ttgcttgccg
     6601 cgcgaacagg aggaaagtcg tctgtgtcaa tcgctcgaga cacggatttg tgatcaccaa
     6661 gcgccgatac cggagtctgt aaaccatcgg gaggatggga accggttgac aggaaggcgg
     6721 acgcctgcgt tgaggacctc ggggtaaccg agaaagcttc tgcttgacgg cggggtgctc
     6781 gactacctgc cggtttcgct catggctgtc gatgcggagg gaccgttcgt ggcgatcgat
     6841 gtgtccgata agccaggtgg cggaacgggg acggtccgtc ctcagattgc aagcctggga
     6901 gcggtggcac ggaagtcctt actggaaggg attcggagat ctccaaactc cccgaaacga
     6961 tgatgcgcac cctcactgtg gggagcatcg acaccgccat ccataccaga caacatgcgg
     7021 acaaggttat ccgtccggaa gtcgagtact gtggaattct ggaatggagt caactcgatt
     7081 cggttcggga ggcgggtcgc aaggcggccc gcggagccct tgagtcgatg ccggaaactt
     7141 tcggaaaggg gagcaatgtc aagccgtaga tcagttatct caggggcggt cgcgatgctg
     7201 gtcctgttga cagcagcggt gctgccggcc acagagacct tcgcgtaccc gtcaggggaa
     7261 ctcgtgcccg gcccggtgaa gcaggtggct tcatcgacct tgcgggcaag gcacctgacg
     7321 gcgttcaact caccggatcg aaagacgttt tgcctttcga gtccgagtta ctcgctcgat
     7381 tgcgggacga tcccgaagtt cccgaggaaa agtccggaac actcagggtg gatcgatgcc
     7441 aggaccggaa aggtcactct ttgcttcatc gccggggcga atctcgcgaa ctcctgcttc
     7501 gtgaactgga acaacaactc tccggttctt catttcggcc agaagaaccg gattggtccc
     7561 ttcagatgcg tttcccggca ccgcggcata acctgcatca aggtgaccgg tcgtggcgcc
     7621 ggcaaggggt tccggatcaa ccgcaagctg gcggtcaagg tccggatttc ggtctgagca
     7681 acgaaaaagg ggcgcccgga gcgggcgccc cttttctttc tcgtggattc cggatggggt
     7741 cagtcgaagt agacctcggc gtcgactccg ccggtgcaca cgtggggtga accgctgcag
     7801 tacatctcgt agtcctggtt catcgtcgga ctgtaggcgg tcacgtaacc gctgccggag
     7861 ccaatctcat cgcagtaggc gtcggcgacg ttctgcgcga aaccgggtgt ggtggtggac
     7921 agcttgtagg acaggttgct tccgcaggag cttccggaat agctgtcgtc ataggacgag
     7981 tcgtaggacg cgtcgtcgta cccggagtcg ccataactcg agtcgtaccc cgagtcatag
     8041 gttgagtcgt cgtaacccga atagtcgtag tccgagctgg tgatggacgg ggtggagtcc
     8101 gagccgttga actcgccctt caccgtcgga gcatccccgc cggagctgcc gctgacccgc
     8161 tcgggctggc tgccgctaca ggcgctaagg gcgactgcgg cgccgatcgc gactataaaa
     8221 gcgatgacga tgcgtgactg gaaattcatg gtgcttcccc ctcatgtgtc ggaaattgtc
     8281 cgagtgcgac acatctggaa atcgcactct gctattccag acggcacctg gtcaggggat
     8341 cttcccgggg tgacgggact cacacaaagg tggccagcca gggtctggct gcggctactt
     8401 cgaaaccgaa aggtcgccag cgagttcggt cttgccgttc agctgatact ggacgtcgat
     8461 cgtttcgtcc ttgttgtaaa agccgaccac tcctagcagc atgaccagtt tcttcttgcc
     8521 ggatttctct tccttgatga tctcccgggt gaaaaggatc tcgttgccgc gcagcacata
     8581 gtcctggtct tcgctctggg cgacgccatt gatgaagacg acgatcggct gttcggcgcc
     8641 ttcggggata gtcacccgcc ttccggcggc gtaggagcct ttggggatct tctgttcggt
     8701 tgcttcttca ctcatcggcg agtcacattc tcgccgattc ggctgcgaag gggatcgtcg
     8761 ccgctggccc gggcgtctac tgcaccttga cgctggccct ggcggacttg ctggtcatgc
     8821 cctgaccgct cgcggtgaag gtaacggtgg cgcgcttgcc cttgttcgcg ttctttttga
     8881 ccgtgatctg aagactggcg gtcttgaagt tgtcggccgc cacagaaccg agactcacgc
     8941 acttgggaat ctggaccagg ttcttagggg ccgaagcgca gactctgacg tttctggccc
     9001 aggcattgcc gacgttatga acggagatct tgaaggttgc cttcttgccg cgacggaccg
     9061 tcttcgaatt cgttgagagc ttcaacgggc cgaagattgg ctttttgcca gagttggggc
     9121 tggtcactac ggcggtcttc tgggacacgg ccgcagtcga agaccctgag ctgttggatg
     9181 cagtttcagt gactctgatt gtcgatccaa catcctgctg ggtgagcacg taggtctgtt
     9241 gatccgctcc cgggatcgcc gaacagtttg ctcccgaact gtcgcaacgc ctccactcgt
     9301 agctgtaact ggttggaata ttggtccact gcccgtgtgt ttccgtca
//
LOCUS       DAOQMF010000002         7707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_3831_length_7707_cov_3.938578, whole genome shotgun sequence.
ACCESSION   DAOQMF010000002 DAOQMF010000000
VERSION     DAOQMF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3831_length_7707_cov_3.938578"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLV77_00055"
     CDS             <1..843
                     /locus_tag="PLV77_00055"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC14272.1"
                     /translation="VETGVSCSSCGRPICPDCMTTTPVGMRCPECATEKTRVRSGPAA
                     TSQVGGYPATIALIAINVVVFLAEMATGSGGLSSTSSDLISDFGLRGAQVADGETYRL
                     VTSGFLHAGFMHIAFNMIALYFLGRVLEPSIGTVRFLMVYFASLLAGSFGAVLLSDNL
                     ALTVGASGAVFGLFAATFIIAYGRGLGHVANEIGLIIGINLLLTFTISGISIGGHLGG
                     LIGGGLCGILVIAGERGYLGSNARLLEYGGMIAIAAVSVFAAISAAEPVPGLHFAVLN
                     LPFL"
     gene            complement(784..1698)
                     /locus_tag="PLV77_00060"
     CDS             complement(784..1698)
                     /locus_tag="PLV77_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNC14273.1"
                     /translation="MRIASLVPSSTEMLFALGFGDQVVAVTHECDFPPEAAALPHLSE
                     TVIEPGLSPAEIDAEVKRVVGQGDPLYRLKAEVLESVRPDLIIAQDVCAVCAVSYDDV
                     LAIAARLPGNPVVLKQDPTSLVEVLGNAIEVAEATGNPEAGVALKAELDARIESVRSA
                     VSGKERPTVLALEWLDPPFTGGHWVPEMIEIAGGRDLVGKAGLKSAEHGWDELANLDP
                     DLTVVMPCGYYVDEAATQADDYMDRIRSTGAERTVAVDAAASFSRPGPRLIDGIELLA
                     GLLHPEVFPLEEGQIQYREMQTGDRFGG"
     gene            1733..2011
                     /locus_tag="PLV77_00065"
     CDS             1733..2011
                     /locus_tag="PLV77_00065"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNC14274.1"
                     /translation="MALLEDQEIQDRLAELDGWSLEGRRIVRTFEFDDFVGSINFVDS
                     LVQPAESMNHHPDLGISWNKVTVAITNHAEGGLTEKDFELASRIQALV"
     gene            complement(2008..2835)
                     /locus_tag="PLV77_00070"
     CDS             complement(2008..2835)
                     /locus_tag="PLV77_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNC14275.1"
                     /translation="MTATPYEFVAIAGSGTIATGLAAVASTSSGKVVMLVRSEESADR
                     ALTAVEKASRRIPGANPSRVTTTMEIADLAEANLLVEAVVEDAGVKADLLRRISEVAP
                     RADLATTTSSLSITKLGEDSGAADRVYGLHVFNPVPAMQLVELIFPDPVTEEVAERAH
                     DWCHILEKTAVEVPDTAGFAVNRLLFPYLFDAVRYQERTGLEAAAVDQCMTLGVAHPM
                     GPLALLDLVGLDVAVAIGQALNNESGNPDHIAPATVQEFVAAGHLGRKTGQGFYEYG"
     gene            2957..4129
                     /locus_tag="PLV77_00075"
     CDS             2957..4129
                     /locus_tag="PLV77_00075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNC14276.1"
                     /translation="MSKFGGREVVIVEAVRTPVGRGHKEKGYYKDTHPSTLLAHAYKN
                     VVERAGIDPGEVEDVVAGCVQQFGEQTLNIGRNAWLEAGFPIETPATTIDRQCGSAQQ
                     AINYAAAMIASGVHDVMIGGGVEHMGHISFAHSAKVMSEYGYAYSPELLEKYDLVNQG
                     ISAEMIADQWEIPKVDLDELAVRSHARAQQATEEGRFEREIAPFQVNGDTYMTDQGIR
                     PGTNLEALAGLKPAFKEDGKITAGNSSQVSDGAAAVLLMTKEKADELGLKARARIVDQ
                     TAVGVDPVIMLTGPIPATRKILERNDMTMDDIDLTEINEAFASVVKAWEIELQPDMDK
                     VNVNGGAIALGHPLGSTGARLMTTLLHELERQDKKTGLVTMCCGGGIGTATLIERI"
     gene            complement(4217..5251)
                     /locus_tag="PLV77_00080"
     CDS             complement(4217..5251)
                     /locus_tag="PLV77_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14277.1"
                     /translation="MNGTFESANGSSGAADGFLRDTATGLTVTPTGLGQTSTGVWTSP
                     EFTYDGAEGEPAETLSFALARKGTLSDLLSQSSANWQVDLVKSSDSSIIPLIPNGPAT
                     NADSWVNIPGSALAADGLTIGDAYRIVITTNVITETLGLAPSGTFDYDEVALTARRAT
                     PTGPTGPTGPTEPTGPTGPTEPTGPTGPTEPTGPTGPTSPTGPTGPTGPTGPNKPVTL
                     TKGNAAFNGKRLFIRLYCPREFKPKCNTVSVPVTRKVANKAPKKIKRLSKPMARPIRV
                     TVKSGKWKKVSYLIKPAYRNKVRKMAGKNKRLLAIRDYIKAKKFAGRKFKGKTRTVFH
                     SHKVVIGKAR"
     gene            complement(5248..5985)
                     /locus_tag="PLV77_00085"
     CDS             complement(5248..5985)
                     /locus_tag="PLV77_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14278.1"
                     /translation="MKLAGLAVMAVAFFGSTAAASADPVVFNFDESIINLGGGSGPQG
                     LKLIDPDADPADAPATLNGEVDTATDAFTAAASGFTFPQKTTQVSGQDVVVTITANAA
                     ITGTFDTDTGTADITVPVTVSAAVLGNTCTTSFPLELKTTGTLVEPGGNHDAAPFDPD
                     TGEGSVYSSFTVPNSTGGALCGVVDGQIGGAGDIWLSGTGGIDEGIVTVYPAMTAPAS
                     PPPTAAGSTAPRTARSAHSSREFSAPR"
     gene            6107..7135
                     /locus_tag="PLV77_00090"
     CDS             6107..7135
                     /locus_tag="PLV77_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC14279.1"
                     /translation="MSAMEKVSIGITGETPEDMAAMARLAEAAGFDCGWAIELFRNAF
                     TQTTFLGAKTESMGIGTAIAWGFVRSPMTMAMSAMDIDEITGGRFRLGLGPGVKRLNE
                     TWHNAEYGRPAPHLRETIESVRAIIHAAAASEPVAYKGEYYDLDIKGWFRSTPPVRAD
                     IPIYTAAVQKGMSRMAGDVADGLIGHIICSARWIEQEIVPAFELGLSRSGRDRSSFEF
                     LPSICVAIDDDYQRGLEAARKTLAFYATVKTYLPLFELHDFGQNAADAGAAFRMGDLA
                     GVAEAISDEMVETYCAVGTPDKVKEKVAEVAQFGDAILPGAPTYFIPNEQITEYNEKI
                     IEVFGQTS"
     gene            7184..>7707
                     /locus_tag="PLV77_00095"
     CDS             7184..>7707
                     /locus_tag="PLV77_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNC14280.1"
                     /translation="MTNGCHRAGVFDYAGWRVAHAGSGERGLLFSLFATMAAATAALN
                     LDAAVLVMTPVVLAVASRRGIGHEAFSISTVHLANSASLLMPVSNLTNIMVFHETDLS
                     FGHFTALMALPWLGAIAVERLLVPRLARPAEVRSPPELATVKPEVSVWPAALLGIVLS
                     GFIVSGPLGVEPLA"
BASE COUNT         1330 a         2398 c         2536 g         1443 t
ORIGIN      
        1 gtcgagacag gcgtttcatg ttcgagctgc ggtcgtccga tctgccccga ctgcatgacg
       61 acgaccccgg tcggcatgcg ctgcccggaa tgtgcgaccg aaaagacccg ggtccgcagc
      121 ggtcctgcgg caacctccca ggtcggcggc tacccggcca cgatcgccct gatcgcgatc
      181 aacgtggtcg tattcctggc ggaaatggcg accggatccg gtggtctcag ctcgaccagt
      241 tcagatctga tcagcgattt cggcctgcgc ggcgcacagg tggccgacgg cgagacttac
      301 cggctggtca ccagcggttt tctccacgcc ggcttcatgc acatcgcctt caacatgatc
      361 gccctttact tcctgggacg ggtactcgag ccttcgatcg gcacggtgcg cttcctgatg
      421 gtctacttcg cgtcgcttct ggccggttct ttcggggccg tcctgctcag cgacaacctc
      481 gccctgaccg tcggtgcctc cggcgccgtc ttcgggctct tcgcggcgac tttcatcatc
      541 gcctacggcc gtgggctcgg ccatgtggcg aacgagatcg gattgatcat cggtatcaac
      601 ctgctcctca ccttcacgat cagcggcatc agcatcggcg gccatctcgg tggtctgatc
      661 ggcggcgggt tgtgcggaat tctggtcatc gccggcgagc gtggttacct gggttcgaat
      721 gcccgcctgc tcgagtacgg agggatgatc gcgatcgccg cagtttcggt attcgccgcg
      781 atctcagccg ccgaaccggt ccccggtctg catttcgcgg tactgaatct gcccttcctc
      841 tagaggaaaa acctcgggat gcaacagacc cgccagcaat tcaattccgt cgatgaggcg
      901 agggcctggc cttgagaacg aggcggcggc gtcaaccgcg acggtccgct ctgctccggt
      961 tgacctgatc cggtccatgt agtcgtcggc ctgggtggcg gcctcatcga cgtagtagcc
     1021 gcagggcatg accacggtca ggtccggatc gaggttggcc agttcgtccc atccatgttc
     1081 agctgacttc aggcctgctt tgccgaccag gtcgcggccg ccggcgatct cgatcatttc
     1141 cggcacccag tggccgccgg tgaagggcgg gtccagccac tcgagggcca gcacggttgg
     1201 tcgttccttg ccggacactg cgctgcgaac cgattcgatc cttgcatcga gttcggcctt
     1261 gagggcaacg ccggcttcgg ggttgccggt cgcttccgcc acttcgatcg cgttgcccag
     1321 gacctccacc agggaggtcg ggtcctgctt gagaaccacc ggattgccgg gcaggcgggc
     1381 ggcgatcgcc agcacatcgt cgtaagagac ggcgcagacc gcgcagacat cctgggcgat
     1441 gatcaagtcc ggccggaccg attccagtac ctcggccttg aggcggtaga gcgggtcacc
     1501 ctggccgacc acccgcttca cttcggcgtc gatctcggcc ggggaaaggc ccggctcgat
     1561 aaccgtctcg ctgaggtggg ggagagcggc agcctcaggc gggaagtcgc actcatgggt
     1621 gacggccacg acctggtcgc cgaagccgag cgcgaagagc atctcggtgg aagaaggaac
     1681 gagggaggca atcctcatgg gtgaaatatg gcagcccggg taggctcgga ccatggcgct
     1741 gcttgaggat caggaaatac aggaccgcct ggctgaactc gatggctgga gcctcgaggg
     1801 ccggaggatc gttcgaacgt tcgagttcga cgactttgtc ggctcgatca acttcgtcga
     1861 ttcgctggtt cagccggccg aatcgatgaa ccatcacccc gacctcggta tctcctggaa
     1921 caaggtcacc gtggcgataa ccaaccacgc cgaaggcggc ctcaccgaga aagacttcga
     1981 gctggcctca aggatccagg cgctggttta gccgtattcg tagaaaccct ggccggtttt
     2041 gcggccgagg tggcctgcag cgacgaattc ctggaccgtg gcgggggcga tgtggtccgg
     2101 gtttcctgat tcgttgttga gtgcctggcc gatcgctacc gcgacatcga ggccgaccag
     2161 gtcgagcagg gcgagggggc ccatggggtg ggcgaccccg agggtcatgc attggtcgac
     2221 cgcggcagcc tccaggccgg tgcgctcctg gtagcggact gcgtcgaaga ggtaggggaa
     2281 gagcagacgg ttgaccgcga aaccggctgt gtcgggcacc tcgacggcgg tcttttcgag
     2341 gatgtggcac cagtcgtggg ctcgctcggc tacctcctcg gtaaccgggt cgggaaagat
     2401 cagttcgacc agttgcatcg ccgggacggg gttgaagaca tggagtccgt agacccggtc
     2461 ggcggccccc gaatcctcgc ccagcttcgt gatcgaaagg gaggaagtgg tggttgcgag
     2521 atcggcccgc ggggcgacct cggaaattcg tctcagcaga tccgccttga ccccggcgtc
     2581 ctcgacaact gcttcgacca ggaggttggc ttcagccagg tccgcgatct ccatggtcgt
     2641 ggtcacccgt gaggggttgg ctccggggat tctccggctg gccttttcga cggcggtcag
     2701 ggcccggtcc gccgactctt cggaacgaac cagcatcacg accttgccgc tcgaggtcga
     2761 tgccacggcc gcgaggccgg tggcgatcgt gccactgccc gcgatcgcga cgaattcata
     2821 cggtgttgcg gtcatccggc tgaaccctat tgagatcgac caacaaccgg gattgactga
     2881 cgagtcaatc agctacgatt gttacaagtt tgcaacccct ctttccagcc atcaatcagg
     2941 agaagaaagg cagaaaatga gcaagttcgg cggtcgtgaa gtcgtaatcg tggaggcggt
     3001 ccgcaccccg gtcggccgcg gccacaagga gaagggctat tacaaggaca cccatccttc
     3061 gactcttctg gcccatgcct acaagaacgt cgtcgagcgg gccgggatcg acccgggcga
     3121 ggtcgaggat gtagttgccg gttgcgtgca gcagttcggc gagcagaccc tgaacatcgg
     3181 acgcaacgcc tggcttgagg ccggcttccc gatcgagacc ccggcgacca cgatcgaccg
     3241 tcagtgcggc tccgcccagc aggcgatcaa ctacgcagcg gcgatgatcg cttccggcgt
     3301 ccacgacgtg atgatcggcg gcggtgtcga gcacatgggc cacatctcct tcgcacattc
     3361 ggccaaggtg atgagcgaat acggctacgc ctattcgccc gagctgctcg agaagtacga
     3421 cctggtcaac cagggcatct cggccgagat gatcgccgac cagtgggaga tccccaaggt
     3481 cgatctcgac gagctggccg ttcgttccca cgcccgcgcc cagcaggcga ccgaagaagg
     3541 tcgcttcgaa cgggaaatcg cccccttcca ggtcaacggt gacacctaca tgaccgacca
     3601 gggcatccgt cccggcacca acctcgaggc gctggccggc ctcaagcccg ccttcaagga
     3661 agacggcaag atcaccgccg gcaactcctc acaggtctcc gacggcgctg ccgcggttct
     3721 gctgatgacg aaggagaagg cagacgagct cgggctcaag gcccgcgccc ggatcgtcga
     3781 tcagaccgcg gtcggtgtcg acccggtgat catgctgacc gggccgatcc cggcaacccg
     3841 caagatcctc gagcgcaacg acatgacgat ggatgacatc gacctgaccg agatcaatga
     3901 ggccttcgcc tcggtggtca aggcctggga gatcgagctc cagcccgaca tggacaaggt
     3961 aaatgtcaac ggcggcgcga tcgccctcgg ccacccgctc ggctcgaccg gcgcccgcct
     4021 gatgaccacc ctgctccatg agctcgagcg tcaggacaag aagaccggtc tggtcaccat
     4081 gtgctgcggc ggcgggatag gaaccgccac gctcatcgaa cgaatctgag tccgtcagaa
     4141 ggcgaaaggg cccgggtctt gtccagagac ccgggccctt tcatttggag ggcacttcat
     4201 ttgggaggga ggttcgttat ctggccttgc cgatcacgac cttgtggctg tggaagaccg
     4261 ttcgggtctt gcccttgaac ttccggccgg cgaacttctt cgccttgatg tagtcgcgga
     4321 tcgccagcag ccgtttgttc ttgccggcca tcttcctgac cttgttccgg taggcaggct
     4381 tgatcaggta ggagaccttc ttccacttgc ccgacttgac cgtcacccgg atcggcctgg
     4441 ccatcggctt cgacaggcgc ttgatcttct tcggggcctt atttgcgacc ttcctggtca
     4501 ccggcaccga aaccgtgttg cacttcggct tgaactcgcg tgggcaatac aggcgaatga
     4561 acagtcgctt gccgttgaag gcggcgttgc ccttggtcag ggtgaccggc ttgttcggtc
     4621 cggtcggtcc cgtggggccg gtggggccgg tcggggaagt cggaccggtc gggccggttg
     4681 gctcggtggg gccggtagga ccggtcggtt ccgtcgggcc agtaggacct gtgggttcgg
     4741 tcgggccagt ggggcccgtc ggaccggtcg gggttgcgcg acgggccgtg agcgccacct
     4801 cgtcgtagtc gaaggttcca gagggggcca ggccgagcgt ctcggtgatc acgttggtgg
     4861 tgatcacgat gcgatacgcg tcgccaatcg tcaggccgtc agccgccagc gcgctgcccg
     4921 ggatgttcac ccaggaatcg gcattggttg cgggaccgtt cggaatcagc gggatgatcg
     4981 aactgtcgct ggacttgacc aggtcgacct gccagttggc gctggactga ctgagcaggt
     5041 cggagagggt gcctttcctg gccagggcaa aactcagagt ctcggccggt tcgccttcgg
     5101 ccccgtcata ggtgaactcg gggctggtcc agactccggt cgaggtctgg ccgaggccgg
     5161 tcggggtgac tgtcaggccg gtggccgtgt cgcgcaggaa gccgtcggcg gccccggagc
     5221 tgccgttggc tgattcgaag gtcccgttca tcgcggggca gagaactccc gggatgagtg
     5281 cgcagagcgg gccgtacgag gtgctgtgga cccagccgcc gtcggtggtg gagaggctgg
     5341 tgccgtcatc gccggataga cggtgacgat gccttcatcg attccgccag tgccggagag
     5401 ccagatgtca ccggcgccgc cgatctggcc gtcgaccacg ccgcagaggg cgccaccggt
     5461 cgagttcggc accgtgaacg agctgtagac gctcccctca ccggtgtcgg ggtcaaaggg
     5521 ggccgcgtca tggttaccgc cgggttcgac cagagtgccc gtggtcttga gttcgagcgg
     5581 gaaggaggtc gtgcaggtat tgccgagtac ggcagcgctg acggtgaccg ggaccgttat
     5641 gtccgcggtg ccggtgtcgg tgtcaaacgt tccggtgatc gccgcgttgg cggtgatcgt
     5701 cacgacgacg tcctgacctg aaacctgggt ggtcttctgg ggaaaggtga agccggatgc
     5761 tgcggcggtg aaagcgtcag ttgccgtgtc cacctcgcca ttcagggtgg ccggggcatc
     5821 ggcggggtcc gcatcggggt cgatcagctt gaggccttgc gggcccgagc cgcctccgag
     5881 attgatgatc gattcatcga aattgaaaac gaccggatct gccgacgcgg cagcggtcga
     5941 gccgaaaaaa gcgaccgcca tgacggccag tcctgccagt ttcatcaaca tcctgttcat
     6001 gcttgccttt cccttggtgc ctgggtttct ctccacttgc gtacggacgg gtacgcaatg
     6061 acccactacc acctggtcgg gatttcaaac agatacgctc aggagcatga gcgctatgga
     6121 gaaggtttcg atcgggatca cgggggagac gccggaggac atggcggcca tggcccggct
     6181 ggctgaagcg gccggtttcg actgcggctg ggcgatcgaa ctattccgca atgcattcac
     6241 ccagacgaca ttcctcggtg cgaaaaccga atcgatgggc atcgggaccg cgatcgcctg
     6301 ggggttcgtg cgcagcccga tgaccatggc gatgagtgcg atggacatcg atgagatcac
     6361 cggcgggcgc ttccggctgg ggctcggccc gggcgtcaaa cggctgaacg agacctggca
     6421 caacgccgag tacggccggc ctgccccgca tctacgggaa acgatcgaat cagtccgggc
     6481 gatcatccac gccgcagcgg ccagtgagcc ggttgcctac aagggcgagt actacgacct
     6541 cgacatcaag ggctggttcc gatcgacccc gcccgtccgc gccgacatcc cgatctacac
     6601 ggcggcggta cagaagggca tgtcgcgcat ggccggtgac gtcgccgacg gtctgatcgg
     6661 ccacatcatc tgctcggccc gatggatcga gcaggagatc gtcccggcct tcgaactcgg
     6721 tctttcccgc tcggggaggg atcgttcttc cttcgagttc ctgccgagca tctgcgtggc
     6781 aatcgacgac gactatcaac gcggtctcga agctgcccgc aagaccctgg ctttctacgc
     6841 gacagtcaag acctatctgc cgctcttcga actccacgac ttcggtcaga acgccgccga
     6901 tgccggtgcg gccttccgca tgggcgacct cgccggagtg gccgaagcga tttcggacga
     6961 gatggtcgag acctattgcg cggttggcac cccggacaaa gtgaaggaaa aggtggccga
     7021 ggtcgcccag ttcggcgacg cgatcctgcc gggcgccccg acctacttca ttccaaacga
     7081 gcagatcacc gagtacaacg aaaagatcat cgaggtcttt ggtcagactt catagatcga
     7141 ggcgatcacc tacacagcag ccttcctggc cgcgctgctc ctcctgacca acggctgtca
     7201 tcgggcggga gtattcgact acgccggttg gcgggtggcc cacgcgggat ctggcgagcg
     7261 cggcctgctg ttcagtctct tcgcgaccat ggcggcggct accgcggctc tcaacctgga
     7321 cgcggcggtg ctggtcatga ccccggtagt gcttgcggtt gcatcgaggc gcggcatcgg
     7381 tcatgaagcc ttctcgatct ccacggtgca cctggccaat tcagcttcgc tgctgatgcc
     7441 ggtttccaac ctgaccaaca tcatggtctt ccacgagacc gacctctcct ttgggcattt
     7501 caccgcgctc atggctctgc cctggctggg cgcgatcgcc gtcgagcggt tgctggtccc
     7561 gcggctggcc cggcccgcgg aagtccggtc accacccgag ctggcgactg tcaaacccga
     7621 ggtaagtgtc tggccggcgg cgctactcgg aatcgtgctt tccgggttca tcgtcagcgg
     7681 tccgctcggg gtcgagcccc tggcgat
//
LOCUS       DAOQMF010000003         7636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_3898_length_7636_cov_3.768104, whole genome shotgun sequence.
ACCESSION   DAOQMF010000003 DAOQMF010000000
VERSION     DAOQMF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3898_length_7636_cov_3.768104"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..800)
                     /locus_tag="PLV77_00100"
     CDS             complement(108..800)
                     /locus_tag="PLV77_00100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC14281.1"
                     /translation="MTPKEAIPFSVDPIRIHLRFSPKGARHISIRVRKLGSGRTIRRI
                     VTGRLAGARWHHIYWDGLTQRRRLAPAGRYLVVAGPIGGPLKRVGRIRLHSHEFPVDG
                     PHGTRGYIGSFGAPRVDGRTHEGFDITAACGTPLVAVRAGRIIKKAYDPELYGNFIVL
                     KGKGERRTYKYAHLIRPAPVRRGQRVRAGRRLGSVGQTGNAAGTPCHLHLEIRTHGHL
                     LNPEPLVNAWDW"
     gene            complement(957..1541)
                     /gene="clpP"
                     /locus_tag="PLV77_00105"
     CDS             complement(957..1541)
                     /gene="clpP"
                     /locus_tag="PLV77_00105"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNC14282.1"
                     /translation="MSPLVPMVVEQTSRGERSFDIYSRLLNERIIFLGTGVNEDIANL
                     VVAQLLHLDAEDPEKDISLYINSPGGSVYAGLAIYDTMQFIKPDVQTICVGIAMSMGA
                     LLLAGGAAGKRMSLPNSKILIHQVSSGFQGQATDIEIHANEIIDTRSRLDKILAKHTG
                     QDLEKITHDTERDYFMSAEEATTYGLIDRVIEHR"
     gene            complement(1662..3038)
                     /gene="tig"
                     /locus_tag="PLV77_00110"
     CDS             complement(1662..3038)
                     /gene="tig"
                     /locus_tag="PLV77_00110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNC14283.1"
                     /translation="MKTTVTELEDSRARIEVEVPADEVSVQVQRAARALAREMRMPGF
                     RKGKAPPSLAIQRLGYDTVFQEAIQEGLPEWYQKSVYASGVVPIGSPDVEITKAPTQE
                     GETVEFKYEVGVRPNAELGEYKGLEVEKAGTEVPDEVIDREIDRMREGMASLDVVDRA
                     AEEGDHVLVDFVGSLDGVEFEGGSANDHTIEIGSGQLIDDFEEQIIGAKAGDEVAVNV
                     NFPEEYGAAELAGQNADFKVTVKEVRVKQMPEADDDFASEASEFDTIEELRADIAEKL
                     GESAGQRIEQDFRVAAVDAAVKNAKVGIPEDILRARGEERWERVERQMAQQGMNPDTY
                     LQMQGKTRDEIIDESLDDADMEIRREAVLVAVADAEGIEVTDEEMAEELEHAASHERT
                     TGAKLLERLKRDGRHEMVIADIKVRKAMDVIADSATATEMSSEETVKKLWGLGDDEED
                     SETEPSSE"
     gene            complement(3093..3665)
                     /locus_tag="PLV77_00115"
     CDS             complement(3093..3665)
                     /locus_tag="PLV77_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNC14284.1"
                     /translation="MSDEYLLIHFEDGEQLGAWLEENGETHDEVWVEIYKKHTGVKSI
                     AWAEIVDTVLCFGWIDSQSRRIDEDRYRQRLTPRRKRSKWSKINCDKAEALIADGRMR
                     PAGLAEVERAKEDGRWEEAYDSPNTATVPEEFEKALEAAGLTEKFSKLKSQERYSMLH
                     RIQTAKRADTKARLIEKFCGMISRGEQVHP"
     gene            complement(3662..3784)
                     /locus_tag="PLV77_00120"
     CDS             complement(3662..3784)
                     /locus_tag="PLV77_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14285.1"
                     /translation="MKRAPIRAWLVTGPPGRLVGFLLDFARALRAHRRAKKAER"
     gene            complement(3786..4187)
                     /locus_tag="PLV77_00125"
     CDS             complement(3786..4187)
                     /locus_tag="PLV77_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC14286.1"
                     /translation="MAGVYLDTSALGHLVLDEPDADVIGSALTRFEVVVSSRLLRTEL
                     HRLGLRTGVPSDEIASWLTSLALVPVDEEILSAAESIGPASVATLDAIHLVTAIRLAA
                     SGHISSVMTFDVRLAEGAAQHQLEVIAPVGE"
     gene            complement(4193..4477)
                     /locus_tag="PLV77_00130"
     CDS             complement(4193..4477)
                     /locus_tag="PLV77_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNC14287.1"
                     /translation="MPKVGVRELRQNLSRHLQRVKAGESLEVTEHGRVVARLSPAGEN
                     LPEAYLALAADHGAQVPSTSLSDLVANRPRRATPAGTVDAMLAEGRAERS"
     gene            complement(4527..6119)
                     /locus_tag="PLV77_00135"
     CDS             complement(4527..6119)
                     /locus_tag="PLV77_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNC14288.1"
                     /translation="MAASPPPGTSGRFLGLLVFTTGACTMGAEIAGARLLAPYFGDST
                     IVWANTIAIVLVALSIGYWIGGRLADRHPDLRSLCGTVLCGAILVTLIPFFARPFLGA
                     SVDAFDELSVGVFAGSLAGVLVLLAIPVMIFGAVSPWALRIAMDNSDVEHAGQLAGRL
                     YALSTAGSLVGTLVSAMVTIPLIGTQRTFIVFALLVAIVASIGLRKLWPALIPAAVAC
                     AALVPVGTIKASSDGEVIFEAETPHQYIRVVETDDGERRLELNEGQAIHSIYRPGEYL
                     VGNYWDEHLVLPFVAPPGVTGARQATWATLPAHPKPLKPWPRRIAILGNAAGTVARAY
                     GHFYPGTKIDAVEIDGKVTEVGRKYFGLDNPNMEVHTEDARPWLEAADPVYDVIMVDA
                     YHQPYIPFYMATKEFFELVRDRLRPGGVVLINVGHPENSDALEENLTATLEEALPEVL
                     RDPVTDTNTMLVGAAGPIEPGNIIQATKGKSSPIPPALDNLATDTAIRIRPPLSGGDI
                     WTDDHAPVEWLVDLSLLDYARE"
     gene            complement(6109..7080)
                     /locus_tag="PLV77_00140"
     CDS             complement(6109..7080)
                     /locus_tag="PLV77_00140"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNC14289.1"
                     /translation="MNLLPTPPLAGISPTVVAMLELIPFTIAVVIYWHRAMTLSWDGR
                     KVPLWRQISFGFGLFLAASMLFNPWGYLAEELVIAHMVEHLLIGDIASLFIVLGLTRS
                     LLQPVLAIPFFNRLQVLTSPVVAFPLWAINMFFWHIPAMYDGAYGGAALHGVEHASFL
                     LFGCLMWMPVFGPLPTPKWFGPGWKVVYVIIVRFTAAILGNVLMWTQVTLYHNYAAGQ
                     AKWGLTAVQDQSTAGVIMMVEGTFLILGVLAWTFFEAAKQSMQKQELIDLAFDRGVDI
                     DEERIERAVKAGHGDLLEKRIRAGGNGAAAGEEAMLVGDPGPNADGR"
     gene            7118..>7636
                     /locus_tag="PLV77_00145"
     CDS             7118..>7636
                     /locus_tag="PLV77_00145"
                     /inference="COORDINATES: protein motif:HMM:NF039283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5911 domain-containing protein"
                     /protein_id="HNC14290.1"
                     /translation="MTPGAGHGHFPRIGSYGFLSDCHTCALVAPDGSVEWFCAPRFDS
                     PSLFGSILDRSAGHFRFGPWDENAPLSRRYEPGTLVMETTWCTETGWVVVRDALSISS
                     WAEQGGPRELMTGHETDRCLVRLVKCIDGEVDMEMVCRPRFKYGTEAAVFSDQGLGQA
                     IAEGAGERLAIAT"
BASE COUNT         1251 a         2487 c         2502 g         1396 t
ORIGIN      
        1 aggaccttgg tggtgctgtt tgtacggcac ctccacggac tacttggctc caggacccgc
       61 gagtttccac tttgcgccac ataccggcac aaagtgggca ttcgtcctta ccaatcccag
      121 gcgttcacga gaggctcggg gttgagaagg tggccgtggg tgcggatttc caggtggagg
      181 tggcaggggg ttccggcggc gtttccggtc tggccgactg agccgaggcg gcggccggcc
      241 ctcaccctct ggccgcggcg gaccggggcc ggccggatca ggtgggcgta cttgtaggtg
      301 cgcctctccc ccttgccctt cagcacgatg aagttgccgt agagctcggg gtcatatgcc
      361 ttcttgatga tcctgcccgc ccgcaccgcg accagcgggg taccgcaggc ggcggtaatg
      421 tcaaagccct cgtgggtgcg gccgtccacc cggggtgcgc cgaagctgcc gatgtagccg
      481 cgggtgccgt gcggcccgtc gaccgggaac tcgtgcgagt gcaggcggat ccgcccgacc
      541 cgcttcaggg gcccaccgat cggcccggca accaccaggt aacggccggc gggggcgagc
      601 cggcgccgct gggtgaggcc gtcccaatag atgtgatgcc agcgcgcacc ggcgagccga
      661 ccggtgacga tgcgacggat tgtccggccg gacccgagct tcctgaccct gatcgaaatg
      721 tgacgggctc ccttcgggct gaagcgaaga tgaatccgga tcgggtcaac cgagaagggg
      781 attgcttctt tcggcgtcac ctcaccggcc aggagccgga atccgcttcc cccttcgccc
      841 gccccggcgt gggaaagttc cgacgtggcg gatgcgtgcc ctgcaaagac cagagccgcc
      901 gccaggatgg cgacggctcc ggtgaaactc ttgaggctcc ctttcaggat gcgcctctac
      961 cgatgctcga ttacccggtc gatcaggccg taggtggtgg cctcttcggc gctcatgaag
     1021 tagtcgcgct cggtgtcgtg ggtgatcttc tcgaggtcct ggccggtgtg cttggcgagg
     1081 atcttgtcga gccggctgcg ggtgtcgatg atctcgttgg cgtggatttc gatgtccgtg
     1141 gcctgaccct ggaaaccgga ggagacctgg tggatcagga tcttcgagtt gggcagcgac
     1201 atccgcttgc ccgcggcgcc accggcgagc agcagggcgc ccatgctcat cgcgatgccg
     1261 acgcagatcg tctggacgtc gggcttgatg aactgcatcg tgtcgtagat cgcgaggccc
     1321 gcgtaaacgg agccgccggg cgagttgatg tagaggctga tgtccttctc gggatcctca
     1381 gcgtccaggt ggagcagctg ggccaccacc aggttcgcga tgtcctcgtt cacgcctgtg
     1441 ccgaggaaga tgatgcgctc gttgaggagc cgggaataga tgtcgaacga acgctctccg
     1501 cgagaggtct gctcgacgac catgggaaca agtggactca tgccgccatc ctatagaggg
     1561 tagttatcgg gccgactcca ccagagccgg cccatccgtc aaatgtctct gtgggccggc
     1621 gggtttctcg tggagggcgg gtcggacccc gcccgacttg cttattccga agagggttcg
     1681 gtttccgaat cctcttcgtc gtccccgagg ccccagagct ttttgaccgt ctcttcggag
     1741 gacatttcgg tggccgtcgc tgaatcggcg atcacatcca tggccttgcg gaccttgatg
     1801 tcggcgatca ccatttcgtg gcggccgtcg cgcttcagtc gttcgagcag cttggcgccg
     1861 gtggtgcgct cgtgggacgc ggcgtgctcg agttcctcgg ccatctcctc gtcggtgact
     1921 tcgatccctt cggcatccgc cactgcgacc aggacagcct cgcggcggat ctccatgtcg
     1981 gcgtcgtcga gcgactcgtc gatgatctcg tcccgggtct tgccctgcat ctggagatag
     2041 gtgtcggggt tcatgccctg ctgggccatc tgccgctcaa cccgctccca gcgttcttcg
     2101 ccccgggccc ggaggatgtc ctcgggaatc ccgaccttgg cgttcttcac cgcggcgtcg
     2161 accgcggcga cccggaagtc ctgctcgatc cgctgcccgg cggattcacc cagcttctcg
     2221 gcgatgtcgg cgcggagctc ctcgatcgtg tcgaactcgg aagcctcgga ggcgaagtcg
     2281 tcatcggcct cgggcatctg cttcacccgg acttccttga cggtcacctt gaagtcggcg
     2341 ttctggccgg ccagctcggc cgccccgtac tcctcgggga agttcacgtt gacggcgacc
     2401 tcgtccccgg ccttggcgcc aatgatctgc tcttcgaagt cgtcgatcag ctggccggag
     2461 ccgatctcga tcgtgtggtc gttggcggag ccgccctcga actcgacccc gtcgagcgag
     2521 ccgacgaaat cgacgaggac gtggtcgccc tcctcggccg cacggtcgac cacgtcgagg
     2581 ctagccatgc cctcacgcat gcggtcgatc tcgcggtcga tgacctcgtc aggcacctcg
     2641 gtgccggcct tctcgacctc gaggcccttg tattcaccga gttcggcgtt cgggcgaacg
     2701 ccgacctcgt acttgaactc gaccgtctcg ccttcctggg tcggagcctt ggtgatctcg
     2761 acgtccggcg agccgatcgg aaccactccc gacgcgtaaa cgctcttctg gtaccactcg
     2821 ggcaggccct cctggatcgc ctcctggaag acggtgtcgt agccgagtcg ctggatcgcc
     2881 agcgagggcg gcgccttgcc cttgcggaaa ccgggcatgc gcatctcgcg ggccagcgcc
     2941 cgggcggccc gctggacctg gacggacact tcgtcggccg gaacttcgac ctcgattcgt
     3001 gccctcgaat cttctagttc agtaacagtg gtcttcaagg tcgaacgagg gtatcgggga
     3061 ccggctttcc gccgcttcca gagccgccgc gcctagggat ggacttgctc gccgcgactg
     3121 atcatgccgc agaacttttc gatcaggcgg gccttggtgt ctgcccgttt cgcggtctgg
     3181 atccggtgga gcattgagta gcgctcctgg gatttcagct tcgagaactt ctcggtcagg
     3241 ccggcggctt ccagcgcctt ctcgaactcc tcggggacgg ttgcagtgtt cgggctgtcg
     3301 taggcctcct cccacctgcc gtcttccttt gcccgttcca cctcggccag accggccgga
     3361 cgcatcctcc cgtcggcgat cagcgcttcc gccttgtcgc agttgatctt cgaccacttg
     3421 ctgcgtttgc gtcgcggggt cagccgctgc cggtaccggt cctcgtcgat ccggcgactc
     3481 tgactgtcga tccagccgaa acagagcacc gtgtccacga tctcggccca ggcgatcgac
     3541 ttcaccccgg tgtgcttctt gtagatctcg acccagacct cgtcgtgggt ctcaccgttc
     3601 tcttcaagcc aggcgcccag ctgttcgccg tcctcgaagt ggatcaggag gtactcgtcg
     3661 ctcatcgttc ggccttcttc gcccgccggt gggcgcgcag ggccctggcg aagtcgagga
     3721 ggaagccgac caggcggccg ggtgggccgg tgaccagcca ggctcggatg ggggcgcgct
     3781 tcatctcact caccgaccgg ggcgatgact tcgagttgat gttgtgccgc gccttcggcg
     3841 agtcgaacat cgaatgtcat caccgacgag atgtgacctg aggctgcgag ccggatcgcg
     3901 gtgacgaggt ggatggcgtc gagcgtcgcg acagacgcgg gcccgatcga ctcggcggct
     3961 gagaggatct cttcgtcgac cggcaccagg gcgaggctgg tcaaccacga tgcgatctca
     4021 tcacttggta cgccagttcg aaggccgagg cgatgaagct cggtacgaag gagacggctt
     4081 gagacgacaa cctcaaaccg ggtcaaagcc gaaccgatca catcggcatc gggctcgtca
     4141 agcaccaaat ggccgagcgc gctggtgtcc agataaacgc ccgccatcgg tttcaggatc
     4201 gctcggcgcg accctcggcc agcattgcgt caaccgtgcc agccggggtc gctcgccttg
     4261 ggcgattcgc gaccaggtcc gacaaactgg tacttggcac ctgggctcca tggtctgcgg
     4321 ccagtgccag ataagcctca ggcaaatttt cacccgccgg ggaaagcctg gctaccacgc
     4381 gcccgtgctc ggtgacttcg agggactcac ccgccttcac gcgctgaagg tggcgactca
     4441 ggttctgtct cagctctcga actcctactt tcggcattgt gcgtcacacc gtagcacatt
     4501 gactgaatcc accacaaaag accttctcac tcgcgggcgt agtccagcag ggaaaggtcg
     4561 accagccatt cgaccggggc gtggtcatcg gtccagatgt ctcccccgct cagaggcggg
     4621 cggatccgaa tcgcggtatc ggtggccagg ttgtcgagcg ccggcgggat aggtgagctc
     4681 ttgcccttgg tggcctggat gatgttgcct ggctcgatcg ggccggccgc gccgaccagc
     4741 atcgtgttgg tgtcagtgac cggatcgcgg aggacctcgg gcagagcctc ctcgagggtg
     4801 gcggtcaggt tctcttccag ggcgtcgctg ttttcgggat ggccgacgtt gatcagcacc
     4861 accccgcccg ggcggagccg gtcccggacc agttcgaaga attccttcgt cgccatgtag
     4921 aagggaatgt agggctggtg gtaggcatcg accatgatca cgtcatagac cgggtcagcc
     4981 gcctcgagcc acggtcgggc atcctcggtg tgaacctcca tgttcggatt gtcgagaccg
     5041 aagtacttcc gccccacttc ggtcaccttg ccgtcgatct cgaccgcgtc gatcttcgtc
     5101 ccgggataga agtgaccgta ggcccgggcg accgtgccgg cggcgttgcc gaggatggcg
     5161 atccgccgtg gccatggctt taacggcttc gggtgagccg gcagagtcgc ccacgttgct
     5221 tgccgggcgc cggtcacccc cggcggcgcc acgaagggca ggaccaggtg ctcgtcccag
     5281 tagttgccga ccaggtactc gcccggccgg tagatcgagt ggatcgcctg cccctcgttc
     5341 agctccagcc gccgctcgcc gtcatcggtt tcgaccaccc gaatgtactg atggggtgtc
     5401 tccgcttcga agatgacctc cccgtcgctg gaggccttga tcgttccgac cgggaccagt
     5461 gcggcgcagg cgaccgcggc cggaatcagc gccggccaga gcttcctcaa cccgatcgat
     5521 gcgacgatcg cgaccagcag cgcgaagacg atgaaagtcc gctgggtgcc gatcaggggg
     5581 atggtgacca tcgccgaaac cagggtgccg accagcgagc cggcggtcga aagcgcgtag
     5641 agccggcccg ccagctggcc ggcatgctcg acgtccgagt tgtccatagc gatccggagc
     5701 gcccagggcg agaccgcacc gaagatcatc accggaattg ccaacaggac cagaacgccg
     5761 gccagtgacc cggcgaagac gccgaccgag agttcgtcaa aagcatcgac cgaggcgccg
     5821 agaaacggcc gggcgaagaa cgggatcagg gttacgagaa tcgccccgca gagcaccgtc
     5881 ccgcaaagcg acctgagatc cgggtgacgg tcggccagcc tgcccccgat ccagtagccg
     5941 atcgaaagtg cgaccaggac gatcgcgatc gtgttcgccc agacgatggt cgaatcaccg
     6001 aagtagggcg cgagcagccg ggctccggcg atctcggcac ccatcgtgca ggcgccggtg
     6061 gtgaagacga gcaggccgag gaaccggccg gaagtccccg gcggcgggct agcggccatc
     6121 ggcattcgga cccgggtccc cgaccagcat cgcctcttcg cctgccgccg cgccgttccc
     6181 gccggcccgg atccgcttct cgagcaggtc tccgtggccg gctttcaccg ccctttcgat
     6241 ccgctcctcg tcgatgtcca caccgcggtc gaaggcgagg tcgatcagtt cctgcttctg
     6301 catcgactgc ttcgccgcct cgaagaaggt ccaggcgagg accccgagga tgaggaaggt
     6361 cccttcgacc atcatgatca cgccggccgt gctctggtcc tggaccgcgg tgaggcccca
     6421 tttggcctga ccggcggcgt agttgtggta gagcgtgacc tgggtccaca tcagcacgtt
     6481 gccgaggatc gccgcggtga acctgacgat gatcacgtag acgaccttcc agccggggcc
     6541 gaaccacttc ggggtcggca ggggcccgaa gacgggcatc cacatcaggc atccgaagag
     6601 cagaaacgag gcatgttcga caccgtggag cgccgctccg ccgtaggccc cgtcatacat
     6661 cgccgggatg tgccagaaga acatgttgat cgcccagagc ggaaaggcga ccaccggact
     6721 ggtcaggacc tgcagccggt tgaagaaggg gatcgcaagc accggctgga gcagggaacg
     6781 ggtcaggccg agcacgatga agaggctggc gatgtcaccg atcagcaggt gctcgaccat
     6841 gtgggcgatg accagttcct cggccaggta gccccagggg ttgaacagca tcgaggcggc
     6901 gaggaagagc ccgaagccga aggagatctg gcgccagagc gggaccttac gcccgtccca
     6961 ggaaagggtc atcgcccggt gccagtaaat gaccaccgcg atcgtgaacg ggatcagctc
     7021 cagcattgcc acgaccgtcg ggctgatccc ggcaagggga ggtgtcggca acagattcac
     7081 ggcctttcag cgtaccggcg ctggtaattt caccacgatg actcccggtg ccggccacgg
     7141 tcactttccg agaattggaa gctatggctt tctctccgat tgccacacct gcgccctcgt
     7201 ggcgccggac ggctcggtcg aatggttctg cgccccgcgt tttgactcgc ccagcctctt
     7261 cggatcgatc ctcgaccggt ccgccggcca tttccggttc ggaccgtggg acgaaaatgc
     7321 tccgctcagc cggcgctacg agccgggcac cctggtcatg gagaccacct ggtgcaccga
     7381 gaccggctgg gtcgtggtcc gcgacgcact ctcgatctcg tcctgggccg agcagggcgg
     7441 gccacgggag ctgatgaccg gccacgagac cgaccgctgc ctggttcggc tggtcaagtg
     7501 catcgacggt gaagtcgaca tggagatggt ctgccgaccg cggttcaaat acggcaccga
     7561 ggccgccgtc ttctccgacc aggggctcgg tcaggcgatc gccgaaggcg ccggcgagcg
     7621 gcttgcgatc gccacc
//
LOCUS       DAOQMF010000004         7055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_4431_length_7055_cov_3.268143, whole genome shotgun sequence.
ACCESSION   DAOQMF010000004 DAOQMF010000000
VERSION     DAOQMF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4431_length_7055_cov_3.268143"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLV77_00150"
     CDS             complement(<1..289)
                     /locus_tag="PLV77_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14291.1"
                     /translation="MTPATLTSADFEVEAFGLIAELEGEADKGSRQTAIDHRLEALRE
                     SWRQLETEDQERCRTLTSRIAALVAARPGIDTGQPASPRRPQTPAEAAGQTV"
     gene            complement(603..962)
                     /locus_tag="PLV77_00155"
     CDS             complement(603..962)
                     /locus_tag="PLV77_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14292.1"
                     /translation="MNMTARPIELSVHNAVVLATAPLLMIVPYLLTFSPGIGYLTFFL
                     GASLMGVSLAGSSPQRPLPLSAQSGFDSAIGIAIFAIGILAGITGQAAMTTIFLVGFG
                     AAHLALTASTRYSARGA"
     gene            1223..2065
                     /locus_tag="PLV77_00160"
     CDS             1223..2065
                     /locus_tag="PLV77_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNC14293.1"
                     /translation="MAKRSRLDLLLAERGTFTSRTAASRAIRAGQVKLGKDGPIALRP
                     SQEVPLDQEIEVLEGQRYVSRGGVKLENGLDALGIDVTGKLCLDVGASTGGFTDCLVQ
                     RGARHVIALDVAYGQIDASLRDRTEITVIERLNARDLLPADLEYRPELTVIDVSFISL
                     TKVLPAVVNTVAEDSTILAMVKPQFELGRARVGRGGVVRNQADRREAVEAVADFALEL
                     GLSLDGIAPTGLPGPKGNLETFVQLGHSKASGSGGESTAGEGSSAGARGADSGAALCE
                     GVEL"
     gene            2062..2865
                     /locus_tag="PLV77_00165"
     CDS             2062..2865
                     /locus_tag="PLV77_00165"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNC14294.1"
                     /translation="MSRPTALLFTHSHAAGMDRAVEVAHAAAERHGWELLASKDAEGS
                     EPSLFIVLGGDGTILHALRSSLDRRVPVFGINFGRVGFLAGVEADQIELGLDRAFAGE
                     YEILDLPGLAISANGETQIALNDASFSRRPRGGVAELSYRIAGEEVGHVRCDGLVAST
                     ATGSTGYNLANHGPILAWGVAGYVVSYIAPHTLTARALVVAPGDVLHVMNHRSRDGVD
                     IAVDGVQIGELKPSAEAEVSYLPGLARLAQLPGSNFYHRIREKFGHLAV"
     gene            2970..4649
                     /gene="recN"
                     /locus_tag="PLV77_00170"
     CDS             2970..4649
                     /gene="recN"
                     /locus_tag="PLV77_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNC14295.1"
                     /translation="MLRELRIENLLLIERAELRLGPGLNVLTGETGAGKTVLAHSLDL
                     LMGGKAKKGIVRSGAPEAWVEGVFDLPPEWHGDEELADLLDRIPAGSEEVVLGRRVSE
                     SGRTSAFVGGRSASAADLQLLATRLIAFYGQHEHRRLTIGSAQLAMVDRAAGLKQQER
                     LNEYGSLWRRRRELARELDELLGRDMARERDLDLLRFELDEIEAASLIEGEKEVLTEE
                     RDRLRHAEGLREAAAGAGGRIRGGEDGEGAVGLLAGATDALAALKGVDADLDSLAGRV
                     ESLALEMEDVGLELGRYLERVEADPERLAELEERLDLIDRLGRKHGGTIESVLAHGAW
                     CREEIERLEGGESKEAALRSELEAAKASLDQAAKGLGRARRKAAGELAAEVTADLEDL
                     AMPGAVLSIDLPPVADGPGPSGGESAEFMLAPNPGLPAQPLRDTASGGELSRVMLALA
                     ASGAGDRRTLVFDEIDAGIGGNTAAAVGERLRQVADGRQVIAITHLPQVASRAAAHFT
                     VEKSTGAGSVCATVTALDEDAVVTEITRMLGAGEGDEAAARHARELVMANR"
     gene            4720..5868
                     /gene="steA"
                     /locus_tag="PLV77_00175"
     CDS             4720..5868
                     /gene="steA"
                     /locus_tag="PLV77_00175"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HNC14296.1"
                     /translation="MSEADAGGVPTSRGRIRLGRKTKDLVKTLGPADVAVIGHRNLDR
                     IAAEDLVASGVKVVLNNDFSSDGRYPNRGPLILVGAGVRLIDFPGIDLFETLVENEQV
                     VVEGDEVKRDGLTVARGVEQLGPDLEQQFERQQSEIHHALADFAENTVNHIQEESELL
                     SGAIELPDTRTDFRDRHVLIVVRGPNYKRDLRALRAYIRDVRPILIGVDGGADAIREA
                     GYRPDMILGDMDSVTDDTLKSGPEVVVHAYADGRAPGAERMKLLGVDHIALPFVGTSQ
                     DVAMLMAYEKGASLIVTVGAHFNLIEFLDKDRAGMSSTFLTRLRIGEVLVDAKGVSRL
                     YNPGLTVGPLMLFLGAFLVLVAIVVLSSPALADLFDLIWLKIKIWLGI"
     gene            5879..6793
                     /locus_tag="PLV77_00180"
     CDS             5879..6793
                     /locus_tag="PLV77_00180"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HNC14297.1"
                     /translation="MGYSARYHAASLAAVFLALAIGILIGSQWGSDVLNNTREDLESS
                     LTSDLNDARDQIDELNQEQDWSNEFGSTVYPLLTANRLRERQVGLIGLGPLPSNVTDS
                     VESALEPAGAELVAVGAIRQPPSLDDLAAELQGTPYRQIASSDEVLVSYGRRVGRQLI
                     RGGRLLNLTRSDLMSQSSGQFDQLDGLIFYRAEPDEIDPEEVDTAEMLDRSIIDGAAT
                     TRARLVGIETTGTDPSTVGFLRDLNLTTVDNLDQPAGKVSLVYALNGAEGAFGVGDGA
                     TRIMPELLNPVAPGDGGQGQNGQGRAEP"
     gene            complement(6772..>7055)
                     /locus_tag="PLV77_00185"
     CDS             complement(6772..>7055)
                     /locus_tag="PLV77_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14298.1"
                     /translation="TEKGDADQVDDPASQLRFEQVEAGPLVQNRQGSQRDEGSGNQYR
                     AERKGKLLAPVVDPGQASPEEPADAGGDDQQADEDGDGETEPGYGSALP"
BASE COUNT         1219 a         2252 c         2433 g         1151 t
ORIGIN      
        1 gcacggtctg gccggcggct tcggccgggg tttgcggccg gcgcggggac gcgggctggc
       61 cagtgtcaat tcccggtcgg gctgccacca gggcagcgat ccgcgaggtc agcgtcctgc
      121 accgttcctg atcttcggtt tcgagctgcc gccacgactc gcgaagagct tcgaggcggt
      181 ggtcgatcgc ggtctgtcgg gatcctttgt ccgcttcgcc ctcgagctcg gcgatcaggc
      241 cgaatgcctc gacctcgaaa tcggcggatg tcagggtggc tggagtcacc ggaccagact
      301 aaagacagca tctgacgccg aaaactcgcc cccgcaccag cccgtcgcca ccgttcgacc
      361 ggtgggtcgg atttccagca ccggcgacgg aaatccgacc cacccgggaa gccgggccgg
      421 tcgccagcac cggggtgggc cgaaaacccc gccgggggaa cggaaatccg acccaccgac
      481 ctttcgcgtc ctgcaagaaa agagccccgc cgggaaagcg gggctcttgg tgttgcttag
      541 ggaggagaga gattcaatga tctcttatgt gttttttgat cgatgggagg taggtgggct
      601 ctttatgcgc cgcgggcgct ataccgcgtt gaagcggtaa gtgccaagtg agcggccccg
      661 aacccgacca ggaagattgt ggtcatggct gcctgtccgg tgatgccggc caggatgccg
      721 attgcgaaga ttgcgatacc gatcgccgag tcgaaaccgg actgggcgga aagcggaagc
      781 ggacgctgcg gactcgaccc cgcaagggaa actcccataa gcgaagcgcc gaggaagaag
      841 gtcagataac cgatcccggg gctgaaggtc agcaggtacg gaacgatcat cagaaggggc
      901 gcagtggcca gtacgactgc gttgtggacg ctgagttcta tgggtctcgc ggtcatgttc
      961 atcgttctcc tgagagcaaa gtagacgacc acttactttt cgtgtgtgag tcggatcaca
     1021 cattgacatg aattcatgtc gaattcacgg ttttcgggtt cagaaagccg gattttccgt
     1081 tacaggcagc cagaaattca ccttcagcca gagtcccgaa gccggtgggt cggatttccg
     1141 tcgctggtac gggaaatccg acccaccgga ggggaggggg cgggtcgggg tttgttttcg
     1201 gtcccggtac ggttctggtc agatggccaa gcgatcccga cttgacctgc tccttgccga
     1261 gcggggcacc ttcacctccc ggaccgcggc gtcccgggcg atccgggccg ggcaggtcaa
     1321 gctcggcaag gacgggccga tcgcactcag gccgagccag gaggtccccc tggaccagga
     1381 gatcgaggtc ctcgaaggcc agcgatacgt ctcgcggggc ggggtcaagc tcgagaacgg
     1441 gctcgacgcg ctcgggatcg acgtcaccgg aaagctctgc ctcgacgtcg gcgcctcgac
     1501 cggaggcttc accgactgcc tcgtccagcg tggcgccagg catgtgattg cgctcgacgt
     1561 cgcctacggt cagatcgacg ccagcctgcg cgaccggact gaaatcacgg tgatcgagag
     1621 gttgaacgcc cgggacctgc tcccggccga cctcgaatac cgccccgagc tgaccgtgat
     1681 cgacgtctcg ttcatctctc tgaccaaggt gttgccggcg gtcgtaaaca cggtggctga
     1741 ggattcgacg atcctggcca tggtcaaacc ccagttcgaa ctgggccggg cgagggttgg
     1801 ccggggcgga gtggtgcgaa atcaggcgga ccggcgggag gcggtggagg ccgtcgccga
     1861 cttcgcgctc gagctcgggc tgagcctgga cgggatcgcg ccgaccggat tgcccgggcc
     1921 caagggcaat ctcgaaacgt tcgtccagct cgggcattcg aaggcaagcg ggtccggggg
     1981 cgaatcgacg gccggggaag gatcatccgc cggggcccga ggggctgaca gcggcgccgc
     2041 tctctgcgaa ggtgtcgaac tgtgagtcgc cccaccgccc tgctcttcac ccactcgcac
     2101 gccgccggca tggaccgggc ggtcgaggtt gcccacgccg ccgccgaacg gcacggctgg
     2161 gaactcctgg cgagcaagga cgccgaggga tctgagcctt ccctgttcat cgtgcttggt
     2221 ggcgacggaa ccatccttca cgccctgcgt tcatctctcg accgtcgggt tcctgtcttc
     2281 gggatcaact tcggccgggt cggattcctc gccggggtcg aggccgacca gatcgaactc
     2341 ggactcgacc gggccttcgc cggcgaatac gagatcctcg acctgccggg actggcgata
     2401 tcggccaacg gcgaaacgca gatcgcgctg aacgacgcga gcttcagccg gcggccgcgc
     2461 ggcggagtgg ccgaactcag ttaccggatc gccggggaag aggtggggca cgtccgctgc
     2521 gacggcctgg tcgcctcgac cgcgaccggc tccaccggat acaacctcgc caaccacggg
     2581 ccgatcctgg cctggggcgt ggccggttac gtggtgagct acatcgcccc gcacaccctg
     2641 accgcccggg cgcttgtcgt ggcgcccggc gacgtgctcc atgtgatgaa ccaccgcagc
     2701 cgggacggcg tcgacattgc ggtcgacggg gtccagatcg gcgagctcaa accctcggct
     2761 gaagccgagg tcagttacct gcccggcctc gcccgtctgg cccagctgcc gggatcgaac
     2821 ttctaccacc ggatcaggga gaagttcggc cacctcgctg tttagcgcgc gctctgctta
     2881 acaggggtcg ggcgaggcgg agcgaagctc gccgacttgc cagttaggcg aacgtttgtt
     2941 cgtccgatgg tcccgttaga acatcagaca tgctgcgcga actacggatc gagaatctgc
     3001 tgctgatcga gcgggccgaa ctcaggctcg gtcccgggct gaacgtgctg accggcgaga
     3061 ccggcgccgg caagaccgtt ttggcccatt ccctggactt gctcatgggc ggcaaggcga
     3121 agaaggggat cgtccgcagc ggcgccccgg aggcctgggt cgagggcgtc ttcgacctgc
     3181 cgccggaatg gcacggggat gaggaactgg ccgacctgct tgaccgcatc ccggccggca
     3241 gcgaagaagt ggtgctgggc cggcgggttt cggagtccgg ccggacctcg gccttcgtcg
     3301 gcggccgcag cgcttccgcg gccgacctgc agttgctggc gaccaggttg atcgccttct
     3361 acgggcagca cgaacaccgg cgcctgacca tcgggtccgc gcagctcgcg atggttgacc
     3421 gggcggccgg cctgaaacag caggagcgcc tgaacgaata cgggtcgctc tggcgaaggc
     3481 gtcgtgaact tgcccgcgag ctcgacgaac tgctcggccg ggacatggcc cgggaacggg
     3541 acctcgacct gctccgtttc gaactggatg aaatcgaggc cgccagcctg atcgaaggcg
     3601 agaaggaagt gctgaccgag gaacgggacc ggctgcgcca cgcggaaggg ctgcgggaag
     3661 ccgcggcggg cgccggtggc cgaattcgag gcggcgagga cggtgagggg gcggtcggtc
     3721 tgctggccgg agcgaccgac gccctggcgg cactgaaagg tgtcgatgcc gaccttgact
     3781 ccctggccgg aagggtcgag agcctcgctc tggagatgga ggacgtcggc ctcgaactgg
     3841 gccggtacct cgagcgggtg gaggccgatc ccgaacggct ggccgagctt gaggagcgac
     3901 tcgacctgat cgaccggctg gggcgaaagc acggcggcac gatcgaatcc gtcctcgccc
     3961 atggcgcctg gtgccgcgag gaaatcgaac gcctcgaagg aggcgaatcg aaggaggccg
     4021 cactgcggtc cgagctggaa gcggcaaaag caagcctcga tcaggcagcg aagggtctcg
     4081 gccgggcccg caggaaggcc gccggcgaac tggcggccga ggttacggca gatctcgaag
     4141 acctggccat gccgggggcg gtcctttcga tcgacctgcc gccggtggcc gacgggcccg
     4201 gtccttcggg cggggaatcg gccgagttca tgctggctcc gaatcccggg cttccggccc
     4261 aaccgctccg cgacaccgca tccggaggcg aactttccag ggtcatgctg gccctggcgg
     4321 cgagcggggc gggtgaccgg aggacgctcg tattcgacga gatcgatgcc ggaatcggcg
     4381 gcaacacggc ggcggcggtc ggggaacggc tgcgacaggt ggcggatggc cgtcaggtga
     4441 tcgcgattac ccacctgccg caggtggcaa gccgggccgc ggctcatttc acggtcgaaa
     4501 agtcgaccgg cgcgggttcg gtctgtgcga ccgtgacggc gctcgacgag gatgccgtcg
     4561 tcaccgagat cacgcggatg ctcggagcgg gcgaaggcga cgaagccgcg gcccgacacg
     4621 cacgcgaact ggtgatggcg aaccggtaac cagcacttcc cttacccttg gggggtgaac
     4681 gcgatcacga aaaggctgcg ggggcggccc gcttccgcca tgtctgaagc ggacgcgggc
     4741 ggggttccga ccagtcgcgg ccggatccgg cttggccgca agaccaagga cctggtcaag
     4801 acgctcggac cggctgacgt cgccgtgatc ggtcaccgca atctcgaccg gatcgcggcc
     4861 gaagacctgg ttgcttccgg agtaaaggtc gtgctcaaca acgacttctc ctccgacggt
     4921 cgctacccga atcgcggacc gctgatcctg gtcggcgccg gtgtccgcct gatcgacttt
     4981 cccggaatcg acctcttcga gaccctggtc gagaacgagc aggtggtggt cgaaggtgac
     5041 gaggtcaagc gggacggcct gaccgtcgcc aggggcgtcg agcaactcgg accggatctc
     5101 gaacagcagt tcgagcgtca gcaatcggag atccaccacg ctctggccga cttcgccgag
     5161 aacacggtca accacatcca ggaggaaagc gaactgcttt ccggggcgat cgaactgccc
     5221 gacacgcgca ccgacttccg cgaccggcac gtgctgatcg tggtccgcgg gcccaattac
     5281 aaacgcgacc tgcgggcgct tcgcgcctac atccgggacg tgcgaccgat cctgatcggt
     5341 gtcgacggtg gcgccgacgc gatcagggaa gccggctacc gccccgacat gattctcggg
     5401 gacatggact cggtgaccga tgacacgctg aagtcggggc ccgaggtggt cgtgcacgcc
     5461 tacgccgacg gtcgggctcc cggggcggaa cggatgaagc tactgggggt cgaccacatc
     5521 gccctgccct tcgtcgggac cagccaggac gtggcgatgc tgatggctta tgaaaagggc
     5581 gcttcgctga tcgtcacggt cggggctcac ttcaacctga tcgagttcct cgacaaggac
     5641 cgggccggca tgtcctcgac cttcctgacc cggctcagga tcggcgaggt actggtcgat
     5701 gcgaagggtg tgagccgcct ttacaacccc ggtctcacgg tcggcccgct gatgctgttc
     5761 ctcggggcct tcctggtgct ggtcgcgata gtcgtgctga gctcgccggc cctggctgat
     5821 ctgttcgatc tgatctggct gaagatcaag atctggctgg gcatctaggc ttcccggaat
     5881 gggttacagc gctcgctacc acgcggcctc gcttgcggcg gtctttctcg ccctcgcgat
     5941 cggcattctg atcggctccc aatgggggtc cgacgtgctg aacaacaccc gcgaggacct
     6001 cgaatcgagc ctgaccagcg atctcaacga cgcccgcgac cagatcgacg agctgaacca
     6061 ggaacaggac tggtcaaatg aattcggcag caccgtctat ccgctgctga ccgcaaaccg
     6121 cctgcgggaa cggcaggtcg gcctgatcgg gctcggtccg ctgccttcga acgtgaccga
     6181 ttcggtcgaa tccgcacttg agccggcagg ggccgagctg gtcgcggtcg gggcgatccg
     6241 tcaaccgcct tcactcgatg acctggctgc ggaactgcag ggcaccccgt accgccagat
     6301 cgcttccagc gacgaggtcc tcgtctccta tggccgccgg gtgggaaggc agctgatcag
     6361 gggaggacga cttctgaacc tgacccgcag cgacctcatg tcccagtcga gcggacagtt
     6421 cgaccagctc gacggcctga tcttctaccg ggccgaaccc gatgagatcg accctgaaga
     6481 agtggacacg gccgagatgc ttgaccggtc gatcatcgac ggtgccgcca cgacccgggc
     6541 ccggctggtc gggatcgaaa cgacgggcac cgacccttcg accgttggct tccttcgcga
     6601 cctcaacctg accacggtcg acaacctcga tcagccggcc ggcaaggtca gcctcgttta
     6661 cgcgctgaac ggagccgagg gggccttcgg ggtgggggac ggcgcgaccc ggatcatgcc
     6721 cgagctgctg aacccggtcg ctcccggtga cggcggccag ggccagaacg gtcagggcag
     6781 ggccgagccg taacctggct cggtttcccc gtcgccatcc tcgtcggcct gctggtcgtc
     6841 cccgccggcg tccgcgggct cctcggggct ggcctgacca gggtcaacta ccggggcgag
     6901 aagcttgcct ttccgctcgg cgcggtactg gttaccgctt ccctcgtcgc gctggctccc
     6961 ctggcggttc tgaacgagcg ggccggcctc gacctgctcg aaccggagct gagacgctgg
     7021 atcatctacc tgatcggcgt cgcctttctc ggtct
//
LOCUS       DAOQMF010000005         7008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_4483_length_7008_cov_3.118079, whole genome shotgun sequence.
ACCESSION   DAOQMF010000005 DAOQMF010000000
VERSION     DAOQMF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4483_length_7008_cov_3.118079"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="PLV77_00190"
     CDS             complement(<1..1857)
                     /locus_tag="PLV77_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC14299.1"
                     /translation="MSNRLAEETSPYLLQHKDNPVAWQPWGPGALAEAQEREVPILLS
                     VGYAACHWCHVMERESFEDEKTAAYMNEHFVPVKVDREERPDIDSIYMEAVQSITGQG
                     GWPLTAFLDPQGVPFHGGTYFPPDTSRGMPSFMMVMEAVIKAWDEKRDEILAQSGDVR
                     SRLGAVSLIGAPEGDLDPELPKRRAANLAEQADMDLGGFGPAPKFPPASALDFMLGQG
                     ETAAAGVTLDRMAAGGIFDQLAGGFARYSVDDRWLVPHFEKMLYDNALLARTYLRAWQ
                     TTGRDRYRWVAQKTLDWAMAEMLDPAGGFYASLDADSEGHEGLFYTWTPAEIEEALGQ
                     DAGEAIEAFGVTAEGHLEGRSVLHLPGGAPPDEIDPGLEELTDRLLAARSARIRPDLD
                     DKVLSSWNSLMISALSEAGAVLGRTDYLEAAQRCADFIWNGMRSPQGNLLRTWRQGEA
                     RLNAYLEDHAFLLEALLDLYQACFDPVVFDRARAVAETMIDRFSDPARGGFFTTSHDH
                     EELIARRKDVGDHPIPASSSSAALGLLRLAAMTGESAYRRKAEEVLKLLAPATSRQPA
                     AFGHLLQALAFYHSGGAELAIAWPADADAEADRPGCAVPAAAEPLVAEARRRY"
     gene            1915..2715
                     /locus_tag="PLV77_00195"
     CDS             1915..2715
                     /locus_tag="PLV77_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14300.1"
                     /translation="MGMEASIFSRFWHDQIETNHQEAIFLLFVGFLLSFAFIRMSTRL
                     MRSPKVPWWPGSIVSEGGLHVHHHVFGIVLMMISGAIAFALDGGGTAFNLCGLAFGVG
                     IGLTIDEFALWLHLEDVYWSEEGRRSIDATVLTALVLALLMFGVRPFEIGSDSIGVLI
                     GSIITALFVFLVVAICFLKERVMHAFVGFFVWPVALYGACRLGKPGSPWAKRRYSERN
                     PSKQKRAEARFRPGRRTDRFKEKFRDLVGGGLIHPETDSPDDGSGTDT"
     gene            complement(2769..4136)
                     /locus_tag="PLV77_00200"
     CDS             complement(2769..4136)
                     /locus_tag="PLV77_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC14301.1"
                     /translation="MSDSGLDPQRIASLTEAQQEIYRNRTGKSAAEYQRAVKVMPGGV
                     PSSFQMMDPWPVYLTEGKGSQVWDVDGNQYTDFHAGFGVMVIGHANPTIGEAVKARID
                     QSTHFAAPTNDSIEVAEELKKRFGLPHWRFINSGTEATMDAVHLARGATGRDMILKVE
                     GSYHGHHDAVMVSVAPPLDQIGTREDPISVPYGEGTPKALTDLTHTVPFNNVDALKAV
                     LENIGDQVAGMIVEPAMMNINIIPPKDGYLQAVRELTEQYGIKLIFDEVKTGATMSHG
                     GATGYYGVTPDMITLAKATCGGYPGGSIGMSDEVAQLVEDDRVKQYGTFNGNPLVMAA
                     AKAALTEVLTEQAYVELNEKNEYLKGRCQEIIDKYGLPAYTEGIGAKGCVIFSPERLY
                     EYRDYAEYVDAELSTLGWLYHMNNGIFMTPGVEEEWTLSVFHTEQDLDNYANAFEAFA
                     QDVTA"
     gene            complement(4274..5725)
                     /locus_tag="PLV77_00205"
     CDS             complement(4274..5725)
                     /locus_tag="PLV77_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC14302.1"
                     /translation="MNFIDDVIQKFPFSQEAVIAIDGEGNRTVFCFSHLFARSLGLSG
                     AMLDRGVRRGDVVMILVGSRIEWVVSMLACFRMGAIALPLNPQLSEHDLAYRVAATNP
                     ALAIGEADYLAGLPDGIPAFDLEDLDRIFDEDLAQATPAKVASLALDDPARIVFTSGT
                     TAEPKGVVHSQRYLMGQAVQAQHWVGAEPDDLCWCTAAPGWSKSSRNSFIAPWLRGAK
                     SLLVEGRFDPATRMDIVRQQEVNVLCQAPTEYRILAKRTTLSPAPSLRRMVSAGEALN
                     PEVIGAFREATGLEIADGYGQTETGAVTGMRPGEAVAGREGSMGRPLPGIETRIVEGE
                     LQVMAATSPTFFHSYIDGSRFEGEWWATGDLVREDDEGYLFHEGRNDDVISSAGYRIG
                     PVEVESALLSHPAVAEAAAIASPDPERGSVVKAVVVLRDRHGDEALAAELQEHCRRET
                     APYKYPRLIEFTDELPKTASGKIRRAELRDRET"
     gene            complement(5961..6197)
                     /locus_tag="PLV77_00210"
     CDS             complement(5961..6197)
                     /locus_tag="PLV77_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14303.1"
                     /translation="MSNETMDSVFIDIELDEPAASDPELAKKLEEVCTVGIFKATENG
                     TEIVRGQLDECVLCYLCEEAAPEGSLRIIKKYEN"
     gene            complement(6194..>7008)
                     /locus_tag="PLV77_00215"
     CDS             complement(6194..>7008)
                     /locus_tag="PLV77_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNC14304.1"
                     /translation="KVVGGVTPGRKGREVHGVPVFDTVEQAVENHGSQIDGSIVTVPP
                     GFTKDAVIEAIDNGIKLIVVVTERIPRGDVAEMVEFANMHDARIIGPNCLGLIVPEVC
                     KMGGIGGPAKDAAKSYSPGVVGVMSRSGGMTTEISSSLTAAGLGVSTAVSIGGDAIIG
                     SSYAELMPYFEADEETRAIVIYTEPGGRMEAQLADWQAENNSRLPIVAFMAGKFMDDE
                     SMKGMTFGHAGTIVEGKEDSATEKIKRLESAGITVVERIDEIPDAVKERIGA"
BASE COUNT         1091 a         2268 c         2348 g         1301 t
ORIGIN      
        1 gtagcgccgg cgggcctccg ccaccagcgg ctccgccgcc gcgggaacag cgcaacccgg
       61 tctatccgct tccgcgtccg catccgccgg ccaggcgatc gccagttcgg cgcctcccga
      121 gtggtagaag gccagggcct ggagcagatg gccgaacgcc gccggctggc gggaggtggc
      181 cggggccagc agctttagta cctcctcggc cttgcgtcga tatgccgatt caccggtcat
      241 ggccgcgagg cggagcaacc cgagcgccgc cgaggaggaa gaagccggga tcgggtggtc
      301 tccgacgtcc ttgcggcggg cgatcagctc ctcatggtcg tgcgaggtgg tgaagaagcc
      361 gccacgcgcc gggtccgaga agcgatcgat catcgtttcc gcgaccgccc tagcccgatc
      421 gaagacgacg ggatcgaagc aggcctggta caggtcgagc agggcttcga gcaggaaggc
      481 atggtcttcg aggtaggcgt tcagtctcgc ttcgccctga cgccaggtcc ggagcaggtt
      541 gccctgcggg ctccgcatcc cgttccagat gaagtcggca cagcgctggg cggcttcgag
      601 atagtcggtc cgtccgagca ccgcgccggc ctcggacagg gccgagatca tcagcgagtt
      661 ccaggaactg agcaccttgt cgtcgaggtc cggccggatc ctcgccgagc gggccgcgag
      721 cagtcggtcg gtcagttcct cgagacccgg atcgatctcg tcgggagggg cgccacccgg
      781 aagatggagg acgctgcgac cttcgaggtg gccttcggcc gtgaccccga acgcttcgat
      841 cgcttccccc gcgtcttgac cgagcgcctc ctctatctcg gccggggtcc aggtgtagaa
      901 aagtccctcg tgaccctcgg aatcggcgtc gagcgaggcg tagaagccgc cggccgggtc
      961 gagcatctcg gccatcgccc agtcgagcgt cttctgggcc acccagcggt accggtcgcg
     1021 accggttgtc tgccaggccc tcagataggt ccgggccagc agggcgttgt cgtaaagcat
     1081 cttttcgaag tggggaacca gccaccggtc atcgaccgag tagcgggcga agccgccggc
     1141 gagctggtcg aagatgccgc cggccgccat ccggtcgagg gtcaccccgg cggctgccgt
     1201 ctcgccctga cccagcatga agtcgagcgc ggaggccggc ggaaacttcg gggccggccc
     1261 gaatccgccg aggtccatgt ccgcctgctc ggccaggttt gcggcccggc gcttcggcag
     1321 ttcggggtcg agatctcctt ccggggcgcc gatcagcgac accgccccga gcctcgagcg
     1381 aacatcgccg gactgggcga ggatctcgtc ccgcttctcg tcccaggcct tgatcaccgc
     1441 ctccatcacc atcatgaagc tcggcatgcc gcgggaagtg tcgggtggaa agtaggtccc
     1501 gccgtgaaat ggcactcctt gcgggtcgag gaaggcggta agcggccagc cgccctgacc
     1561 ggtgatcgac tggaccgcct ccatgtagat cgagtcgatg tccggccgct cctcccggtc
     1621 gaccttgacc ggcacgaagt gctcgttcat gtaggccgcg gtcttctcgt cctcgaagga
     1681 ttcccgctcc atcacgtggc accagtggca ggcggcatat ccgaccgaaa gcaggatcgg
     1741 cacctccctt tcctgcgcct cggccagcgc cccgggcccc cagggctgcc acgcgaccgg
     1801 gttgtccttg tgctggagga ggtacgggct ggtctcttcg gcaaggcggt tgctcattga
     1861 cacatgatgg gggttcggac atgcgggcgt tggtcgtggc aggattgggg ggcgatggga
     1921 atggaggcgt cgatcttcag caggttctgg catgaccaga tcgagaccaa ccatcaggaa
     1981 gcgatcttcc tgctgttcgt gggcttcctg ctttccttcg ccttcatccg gatgagcacg
     2041 cggttgatgc gatccccgaa ggttccgtgg tggccgggca gcatcgtcag cgagggcggg
     2101 ctacacgttc atcaccacgt cttcggcatc gtcctgatga tgatctcggg ggcgatcgcc
     2161 ttcgcgctcg acgggggagg caccgcattc aacctctgtg gcctcgcctt cggggtcggc
     2221 atcggtctga ccatcgacga gttcgcgctc tggcttcacc tcgaggacgt ctactggtcc
     2281 gaagagggtc gtcgctcgat cgacgcgact gtcctgactg cgctggtcct ggcgctgctc
     2341 atgttcgggg tccgaccttt cgagatcggt tcggactcga tcggcgtcct gatcggcagc
     2401 atcatcaccg cccttttcgt ctttcttgtg gttgcgatct gcttcctcaa ggagagggtg
     2461 atgcatgcct tcgtcgggtt cttcgtctgg ccggtcgccc tttacggcgc ctgccggctg
     2521 ggcaagcccg gctcgccgtg ggcgaaacgc cgctactcgg aaaggaatcc ctcaaagcag
     2581 aagcgggccg aggcgcgctt tcgccccggc cgccgcaccg accggttcaa ggagaagttc
     2641 cgcgatctgg tcggcggcgg gctgatccac cctgaaaccg actccccgga cgacgggtcg
     2701 ggtaccgaca cctgaagacg gccggaggct ggccggccgg cgatcactcg accgccggcc
     2761 gcgcctgatc aggcggtgac gtcctgcgcg aacgcctcga acgcgttggc gtagttgtcg
     2821 aggtcctgct cggtgtggaa gaccgaaaga gtccactctt cctcgacgcc gggagtcatg
     2881 aagatgccgt tgttcatgtg gtagagccag cccagggtcg agagctcggc gtccacgtac
     2941 tcggcgtagt cgcggtactc atagagccgc tcgggcgaga agatcacgca gcccttggcg
     3001 ccgatgccct cggtgtacgc aggcaggccg tacttgtcga tgatctcctg gcagcggccc
     3061 ttgaggtact cgttcttctc gttcagctcg acgtaggcct gctcggtcag gacctcggtc
     3121 agggcggcct tcgcggctgc catgacgagt gggttgccgt tgaaggtgcc gtactgcttc
     3181 acgcggtcgt cctcgaccag ctgggcgacc tcgtccgaca tgccgatcga gccgcccggg
     3241 taaccgccgc acgtcgcctt ggcgagggtg atcatgtccg gggtcacgcc gtagtagccg
     3301 gtggcgccgc cgtgtgacat ggtcgcgccg gtcttgacct cgtcgaagat cagcttgatc
     3361 ccgtactgct cggtcagctc gcgaacggcc tggaggtaac cgtccttcgg cgggatgatg
     3421 ttgatgttca tcatcgccgg ctcgacgatc atcccggcga cctggtcgcc gatgttctcg
     3481 agcacggcct tcagcgcgtc aacgttgttg aaggggacgg tgtgggtgag gtcggtcagc
     3541 gccttcgggg tgccttcgcc gtagggaacg gagattggat cctcgcgggt gccgatctgg
     3601 tccaggggcg gggcgaccga gaccatcacc gcgtcgtggt ggccgtggta ggagccctcg
     3661 accttgagga tcatgtcgcg gccggtggcg ccgcgggcga ggtggacggc gtccatggtc
     3721 gcttcggtcc ccgagttgat gaaacgccaa tggggaagcc cgaagcgctt cttcagttct
     3781 tccgctactt cgatcgagtc attggtcggg gcggcaaaat gggtgctctg gtcgatccgg
     3841 gccttgaccg cctcgccgat cgtcggattc gcgtggccga tcaccatcac gccgaaaccg
     3901 gcgtggaagt ccgtgtactg gtttccgtcg acgtcccaga cctgggaacc cttgccctcg
     3961 gtcaggtaga ccggccaggg gtccatcatc tggaaggagg aaggcactcc ccccggcatc
     4021 actttcaccg cgcgctggta ttccgcggcg gacttgccgg tgcgattgcg atagatctcc
     4081 tgctgcgcct cggtcagtga ggctattcgc tgggggtcaa ggccgctgtc agacatctgc
     4141 tctcctcggt cgtggggtag ggatcagcct atatatctgc ccgccgccga tccagactca
     4201 aacgtcagag gttccggacc gggctggaca ttttgacaag cagagccgac ccggccatct
     4261 tcaggtcttg cggtcaggtt tccctgtccc tgagttctgc ccttcggatc ttgccggagg
     4321 ccgttttcgg gagctcgtcg gtgaactcga tcagccgggg gtacttgtag ggcgcggttt
     4381 cccggcggca gtgttcctgc agttcggcgg ccagggcctc gtcaccgtgg cggtcgcgca
     4441 gcaccaccac ggctttcacg accgaaccgc gttccgggtc gggcgaggcg attgccgccg
     4501 cctcggccac cgcgggatgc gagagcagcg ccgattccac ctcgaccgga ccgatccggt
     4561 agccggccga ggagatcacg tcatcgttcc ggccttcgtg aaagagataa ccctcatcgt
     4621 cctcgcggac caggtcgccg gtcgcccacc actcgccttc gaagcggctt ccgtcgatgt
     4681 agctgtggaa gaaggtgggg gacgtggctg ccatcacctg gagttcgccc tcgacgatcc
     4741 gggtctcgat gccgggcagc ggccggccca tcgacccttc gcgaccggcc acggcctcgc
     4801 cgggccgcat gccggtcacg gcgccggtct cggtctggcc gtaaccgtcg gcgatctcga
     4861 gtccggtcgc ctcgcggaag gcaccgatca cttcggggtt cagcgcctcg ccggccgaga
     4921 ccatcctccg cagggagggg gcgggggaga gagtggttcg cttggccagg atccggtact
     4981 cggtcggggc ctggcagagc acgttgacct cctgctggcg gacgatgtcc atccttgtgg
     5041 ccgggtcgaa ccgaccctcg accagcagcg acttcgctcc cctcagccag ggagcgatga
     5101 aggagttccg cgaagacttt gaccagcccg gggcggcggt gcaccagcaa aggtcgtccg
     5161 gctcggcccc gacccagtgc tgggcctgaa cggcctggcc catcagatag cgctgcgagt
     5221 ggactacgcc cttcggttcg gcggtggtgc cggaagtgaa gacgatccgg gccgggtcat
     5281 ccagagccag gctggcaacc ttggccggcg tcgcctgggc gaggtcctcg tcaaagatcc
     5341 ggtcgaggtc ttccagatcg aaggcgggta tcccgtccgg caggccggcc aggtagtcgg
     5401 cctcgccgat cgccagagca ggattggtgg cggcgacccg gtaggcgagg tcgtgctcgc
     5461 tgagctgggg gttgagcggc agcgcaatcg cccccatccg gaagcaggcc agcatcgaaa
     5521 cgacccactc gatcctgctt ccgaccagga tcatcacgac atccccgcgc cgcactcccc
     5581 ggtccagcat cgcccccgaa aggcccagac tccgcgcaaa aaggtgagaa aaacagaaga
     5641 ccgtccggtt cccctcaccg tcaatcgcaa taacggcctc ctgcgaaaaa gggaacttct
     5701 ggattacgtc atcaatgaag ttcaaggtcc gccgagtcta gccagccttg gcgcttgcgt
     5761 gaccgggtcc ggtcagtgcg ccccgacgag tttcgggtcg gacaatcgag atgcccgggc
     5821 gacctggttg cgggagtgcc cgccgacgac gtttcgggcc accctttcga acgagtttgg
     5881 ttgagcgggt ccgggcagtg cgccccgacg acgtttcggg ccgaggaggg gtgtactgcc
     5941 cggacccgct ctaaccgaag ctagttctcg tatttcttga tgatccgcag ggatccctcg
     6001 ggagcggctt cttcacagag gtagcagagg acgcactcgt cgagctggcc gcgaacaatc
     6061 tcggtgccgt tctcggtcgc cttgaagatg ccgacggtgc agacctcttc cagcttcttg
     6121 gccagctcgg gatccgaggc ggcgggctcg tcgagctcga tgtcgatgaa gacgctgtcc
     6181 atcgtttcgt tgctcatgct ccgatccttt ccttgactgc gtcggggatc tcgtcgatcc
     6241 gctcgaccac ggtgatgccg gccgattcca gacgcttgat cttctcggtc gccgagtctt
     6301 ccttgccctc gacgatggtg ccggcatggc cgaaggtcat gcccttcatc gactcatcgt
     6361 ccatgaactt gccggccatg aaagcgacga tcggcaggcg ggaattgttc tcggcctgcc
     6421 agtcggcgag ctgggcttcc atgcggccac ccggctcggt gtagatgacg atcgccctgg
     6481 tttcctcatc agcctcgaag tacggcataa gctcggcgta gctcgagccg atgatcgcgt
     6541 cgcctccgat cgagacggcg gtcgagacgc ccaggccggc ggccgtgagc gaggaggaga
     6601 tctcggtggt catgccgccg gagcgggaca tcacgccgac gacaccgggg ctgtacgact
     6661 tggcggcgtc cttggccggg ccgccgatgc cgcccatctt gcatacctcg gggacaatca
     6721 ggccgaggca gttcggaccg atgatccggg cgtcatgcat gttggcgaac tcgaccatct
     6781 cggccacgtc gccgcgcggg atgcgttcgg tgacgacgac gatcagcttg atcccgttgt
     6841 cgatcgcctc gatcaccgcg tccttggtga agccgggggg gacggtgacg atcgagccgt
     6901 cgatctggct gccatggttt tcaaccgcct gctcgaccgt gtcgaagacc ggcacgccgt
     6961 ggacttcgcg gcccttgcgg ccgggggtca cgccgccgac gaccttcg
//
LOCUS       DAOQMF010000006         6841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_4635_length_6841_cov_3.564103, whole genome shotgun sequence.
ACCESSION   DAOQMF010000006 DAOQMF010000000
VERSION     DAOQMF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4635_length_6841_cov_3.564103"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PLV77_00220"
     CDS             <1..1098
                     /locus_tag="PLV77_00220"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC14305.1"
                     /translation="AGIESLDYPKTKLDVRLLCEEDDEETIQAIMDLDLPPHFNAIVV
                     PPSQPQTKPKACNFGLLGAKGEYIVIYDAEDRPDPLQLKKAVYMFDNVDDSIVCIQSK
                     LNFFNQQTNILTRWFSIEYSMLFDLVLPGLDARRDPIPLGGTSNHIKRDELVEVGGWD
                     PYNVTEDADLGVRLHQAGYRTTMMDSTTYEEANTSIPNWIRQRSRWIKGYLQTWLVYM
                     RNPFRFLSNVGLKGFLSFQLLIGGTFMFLINPIFWFLTTIFALTQLGIIEDFFPGWVY
                     FLAASQLFFGNFVFVYLGLAASVRRGDYSLAPYALFLPFYWGLMSVAAWKGFGQLVTN
                     PFYWEKTEHGLDLGGGAEHAHTTTPRKAGTV"
     gene            1098..3047
                     /locus_tag="PLV77_00225"
     CDS             1098..3047
                     /locus_tag="PLV77_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14306.1"
                     /translation="MADERIDINPVIDDGGTAAVQEEVRTRRVRRTYVDPEVASSIGE
                     VPPEPRPFSPDAPRANWESGIVFAVFAIIYGVLGYFMLTDGRIVDFISLAHLNEAYMV
                     FWNDPPRLAAIGLDAATFSSIAYMPLSLVKPLATSLVAMPVLGAITAGLLMASLNSLM
                     RICEINLIFRIVLLILFGLNPMFAFFAASGDPETLGLCLLAIALSSTIAWSVTDQTRH
                     IAGAGLAMGLAVMVDYGYILVGLGFMVAIMIISASKNDDGLKTRSSLLLFLTPVAYAL
                     LFWCLMNWVLLGDPFQWVTAQNGVIQVNTTGALQAITTDVGGSLGDLFEVTLGVAPLA
                     LVAGFLLIIGSFLKKDATGWGLLVVGLCIAAAPVIRAVAADQADLLTISYGLPLAVFA
                     VGAVAWFGKDGWGVVAAVILGVGLIAAIPLSWNTMRDYRFQNQAEAFTRWVEHRDSQE
                     GTKSLGDYTVGIDPELSMARFINNKIPQEKRSILVDQNFSYGVMITSGRPQNFLDRVD
                     AGEDDWQATIDNPWGKDGERDVSYMLITTSREGDQIAKKWPTAVQGGEAGFTPIFRTD
                     RYVLVKVSETRPPSDESDQQSNEPRTTPNPVTPTSPLNPNGPDLTTPSQSETDVTPVS
                     PSPSNNGSTVEPNNGTSSAPSLEGE"
     gene            3047..4330
                     /locus_tag="PLV77_00230"
     CDS             3047..4330
                     /locus_tag="PLV77_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14307.1"
                     /translation="MPNRSMQRWILVIALTALLGLLVAACGSNDSDNGDSGDNSAKST
                     TGGTGATGDTAATANETTGATEPGTGSAVTAGTDPVAEGRLFSADSPWNTTAEGLPID
                     DASDRMLELAQFRRAAKEVPGQEGVQTFTRKIVDDGIFVNSQVWAPLIVAANTQDSVN
                     TKFVCRQSKCSSADPKVPSTLNIPANTTPDPRYDGWLSVIDRENGMGYDFWRARREGD
                     GTISYQFGKAWTLDGPGFSKPVSIDPDRAVGARGSGLPLFAGVIGPQELERGEISHAL
                     AISVPGLARRNFVQPASVTDGNGDRKSLPAGARIRLRADVSINEKKLRTLKGQRRRSA
                     DAILTALRTYGAIVVDRAAVPSLYAPLGTSPKLLRGDELDWLKLDDFEVISLPQIYKD
                     PPLSQVGLEGPETGGDNSQVQSGSAASGNEVGGGL"
     gene            4336..5757
                     /locus_tag="PLV77_00235"
     CDS             4336..5757
                     /locus_tag="PLV77_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNC14308.1"
                     /translation="MLKNKRAGIACASAVLMLLVAGCGVNPDEAPESAGHDGSDANFV
                     PAQTGNSKTKEAKPTPREVGTIRIAGKAQGSLTPDLVRTYNAAGGTANVTVDEEGENQ
                     AFQQFCAGKADVVDSARPISEAEYRKCIANGIQPVQFQVASDAVVLAIKNETDVGTDC
                     LSFDEVRNIFRAGSDINSWSQVGYGHDLGGDTNEPRMKVAGPDEDSNVFGFFGQYVLG
                     APEPTMLDFRSDYQAFPTDAGVRRAVVGSNRDFRAAAQFQRSRQVYQGILDSLAENRR
                     AVRDARAEVAKGIADERPPEDQARDRRKLAKAQANLENLEKSLPAARRYARQNRAALR
                     RLAEVRGTLGLFRFSYYEAFEEQLRPMEVSASNDFAQPECIFPSQATVTNATYPLARQ
                     LLLTINYKNMTDQDINAFLVQSLSRSQQLAEDAALVPLPDETQATELAWLNGTSEPDI
                     IFYESGAGTSVGTQQEADSASGE"
     gene            5901..>6841
                     /locus_tag="PLV77_00240"
     CDS             5901..>6841
                     /locus_tag="PLV77_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14309.1"
                     /translation="MPAASGAAAKKKRVGPCSTLPINTKLSTHDPTGLLEDGYAWLYL
                     NPKGTVRSPNVKVVRHGRVFARGRIVGSLVPGRTTLVKMHVGRRMQQGKYRIRVTASK
                     PGRCSKRITKTRVWKFHRPTLGLKAIPVSTRIGDNVGVVRFALRPVRRTKVGWTRVSL
                     IGPGGGVVSQQVIKSIGSKQILAELPIKGKLKPAKYRVRLTGQDLASGIFRASSQTWR
                     FGKGGGGAKPVPTTGQLTQKVAVDWTNGKWDGRQVGGFIAPGIGYGEVVCSPYQQWVR
                     FYPSDTSRESAMMTWTYKNWDFGKGYEKAVREAKYTEG"
BASE COUNT         1287 a         2218 c         2085 g         1251 t
ORIGIN      
        1 gcgggtatcg agtctctcga ctacccgaag accaagctcg acgtgcgtct gctctgcgag
       61 gaagacgatg aggaaacgat tcaggcgatc atggacctgg atctgccgcc tcacttcaac
      121 gcgatcgtcg ttcccccgtc acagccgcag accaagccga aagcctgcaa cttcggcctg
      181 ctcggtgcca agggcgaata catcgtcatc tacgacgccg aggaccgccc ggatccgctc
      241 cagttgaaga aggccgtcta catgttcgac aacgtggacg actccatcgt ctgcattcag
      301 tcgaagctca acttcttcaa ccagcagacc aatatcctca ctcgctggtt ctcgatcgag
      361 tactcgatgc tcttcgacct ggttctgccc gggctcgacg cccgccgcga tccgatcccg
      421 ctcggtggca cctcgaacca catcaagcgg gacgaacttg tcgaagtggg gggctgggac
      481 ccgtacaacg tgaccgagga cgcggacctc ggcgtccgtc tccatcaggc cggataccgc
      541 accacaatga tggattcgac cacttacgaa gaggcgaata ccagcatccc gaactggatc
      601 cgccagcgct cccgctggat caagggctac ctgcagacct ggctcgtcta catgcggaat
      661 ccgttccgct tcctctccaa cgtcggcctc aagggattcc tttcattcca actgctgatc
      721 ggcggaactt tcatgttcct gatcaacccg atcttctggt tcctgaccac catcttcgcg
      781 ctgacccagc tcggcatcat cgaggacttc ttccccggct gggtgtactt cctcgccgcg
      841 tcacagctct tctttggcaa cttcgtcttc gtttacctgg ggctcgcggc ttcggtccgg
      901 cgtggtgatt acagcctcgc gccctacgcg ctcttcctgc cgttctactg gggcctgatg
      961 tctgtcgcgg cctggaaggg cttcggtcag ctcgtaacca acccgttcta ctgggagaag
     1021 actgaacacg gactcgacct cggcggcggt gccgaacatg cccacacgac gactccgcgg
     1081 aaagcgggca ccgtctgatg gccgacgagc ggatcgacat taatcccgta attgacgacg
     1141 ggggtaccgc cgcggttcag gaagaagtca ggaccagaag ggtccgtcgc acctatgtcg
     1201 accctgaggt cgcgtcaagc atcggtgaag tcccgcccga accgcggcct ttcagcccgg
     1261 acgcgcccag ggccaactgg gagagcggca tcgtcttcgc ggtcttcgcg atcatctacg
     1321 gcgtcctcgg ctacttcatg cttaccgacg ggcgcatcgt cgatttcatt tcgctggcgc
     1381 acctcaacga ggcctacatg gtcttctgga acgaccctcc gcgcctggcg gctatcggcc
     1441 tggatgccgc gaccttcagt tcgatcgcct acatgccgct cagtctggtc aagccgctgg
     1501 cgacctcgct ggtcgcgatg ccggttctgg gggcaattac ggccggtttg ctgatggcat
     1561 ctttgaactc gctgatgcgg atttgcgaga tcaacctgat cttccgcatc gttctgctga
     1621 tcctgttcgg cctgaatccg atgttcgcgt tcttcgccgc cagcggcgac ccggagaccc
     1681 tcggtctgtg cctgctcgcc atcgcactct catcgacgat cgcctggtcg gtcactgacc
     1741 agacccgtca catcgctggt gccggtctgg ccatggggct ggccgtaatg gtcgattacg
     1801 ggtacatcct cgtcggactg ggcttcatgg tggcgatcat gatcatcagc gccagcaaga
     1861 acgatgacgg actgaagacc cgctccagcc tgctgctctt cctgaccccg gtcgcctacg
     1921 cgttgctctt ctggtgcctg atgaactggg tgctcctggg tgacccgttc cagtgggtga
     1981 ccgcccagaa cggggtcatc caggtcaaca ccacgggggc cctgcaggcg atcaccaccg
     2041 atgtaggggg cagtctcgga gacctgtttg aagttaccct tggggtggcg cctttggctc
     2101 tggtagcggg attcctgctg ataatcggct cgttcctgaa gaaggacgcg accggctggg
     2161 gcctgctggt agtcgggctc tgcatcgctg ccgcgccggt gatccgggcc gtcgccgccg
     2221 accaggcgga tctgctcacc atttcctacg gtctgccgct tgcggtgttc gcggttggtg
     2281 cggtggcctg gttcggcaag gatggctggg gggtcgtcgc tgcagtaatc ctcggggtcg
     2341 ggctgatcgc ggcgataccg ctgagctgga acacgatgcg ggattaccgg ttccagaacc
     2401 aggcggaggc atttacccgc tgggtcgagc atcgcgacag ccaggagggc accaagtctc
     2461 tgggcgacta cacggtcggc atcgatccgg aactctccat ggccagattc atcaacaaca
     2521 agattcccca ggagaaacga tcgatcctcg ttgatcagaa cttcagctac ggcgtgatga
     2581 tcacctccgg gcgtccccag aacttcctcg accgggtcga cgcgggtgaa gatgactggc
     2641 aggcgacgat tgacaacccc tggggcaagg acggcgagcg ggacgtcagt tacatgctga
     2701 tcaccaccag ccgcgaaggc gatcagatcg ccaagaagtg gcctaccgcc gtccagggcg
     2761 gagaagcggg gttcaccccg atattccgaa ctgaccgcta cgtgctggtg aaagtctccg
     2821 agaccaggcc gccttcggat gagagcgatc agcagtcgaa tgagccgcga acgactccga
     2881 atccggtcac cccgacttcg cccctgaatc cgaacggtcc ggatctgacc acaccgtccc
     2941 agtctgaaac ggatgtgact ccggtttcgc catcgccgtc caataacggc agcacggttg
     3001 aaccgaacaa cggtacgtcc tccgccccga gcctggaggg tgagtagtgc cgaatcgcag
     3061 catgcagcgc tggatcctcg tgatcgcgtt gacggccctg ctcggcctgc tggtcgctgc
     3121 ctgcggttcg aatgattcgg acaatggtga ttccggcgac aactcggcca agtcgactac
     3181 cggcggcacc ggagcaactg gcgataccgc tgcgaccgcg aacgaaacga ctggtgcaac
     3241 cgagcccggc accggttccg cggtgaccgc tggcaccgat ccggtcgccg agggccgcct
     3301 tttctcggcc gacagtccct ggaacacgac ggccgagggt ctgccgattg atgatgcctc
     3361 cgaccggatg ctggaactag cccaattccg aagggctgcg aaggaggttc ccggccagga
     3421 aggcgttcag accttcactc gaaagatcgt cgacgacggc atcttcgtca actcgcaggt
     3481 ctgggctccc ctgatcgtcg cagccaacac ccaggattcg gtcaacacca agttcgtctg
     3541 ccgtcagtcg aagtgcagct cggctgatcc caaggttccg agcacgctga acatccccgc
     3601 gaacaccact cccgacccgc gctacgacgg ctggctcagc gtgatcgacc gggaaaacgg
     3661 tatgggatat gacttctggc gtgcccgccg tgaaggcgac ggcaccattt cctaccagtt
     3721 cggaaaggcc tggaccctgg atggccccgg cttcagcaag ccggtcagca tcgacccgga
     3781 ccgggcggtt ggtgcgcgtg gatccgggct gccgctcttc gcgggtgtta tcggccccca
     3841 ggaactggag cggggcgaga tcagccatgc cctggcgatt tcagttcccg gcctcgcccg
     3901 tcgcaatttt gttcagcccg cttcggttac tgacggcaac ggcgatcgca agtcgctgcc
     3961 ggccggggcc cggatccgtt tgcgcgcaga cgtgtcgatc aacgagaaga agctccgcac
     4021 cctgaagggg cagcgtcgac gctcggccga cgcgatcctg accgcgctca ggacctacgg
     4081 cgcaatcgtg gttgatcggg ccgcggtgcc cagcctctac gccccgctcg gtacctcgcc
     4141 gaagctgctc cggggtgatg aactcgactg gctgaagctg gacgattttg aagtgatttc
     4201 gctgcctcag atctacaagg atccgccact aagccaggtc ggcctcgaag gtcccgagac
     4261 cggcggtgac aattcgcagg ttcagtccgg cagcgccgcg tctggcaacg aagtcggagg
     4321 tggactgtga gtcgggtgct gaaaaacaag agggccggta tcgcctgcgc atcagccgtg
     4381 ctgatgctgc ttgtcgccgg ctgtggcgtg aatccggatg aagcacctga gtcggcgggt
     4441 cacgacggct ccgacgcgaa cttcgtgccg gcccagaccg gcaacagcaa gaccaaggaa
     4501 gccaaaccca ccccgcgtga ggtcgggacc atccggatag ccggcaaggc ccagggttcc
     4561 ctgacccccg atctggtcag gacctacaac gcggctggtg gcaccgcaaa cgtgaccgtc
     4621 gacgaagaag gcgagaacca ggccttccag cagttctgcg ccggcaaggc cgatgtcgtc
     4681 gattcggccc ggccaatctc cgaagccgaa taccgcaaat gcatcgccaa cggcattcag
     4741 ccggtccagt tccaggtcgc ctcggacgcg gtcgtccttg cgatcaagaa cgagaccgat
     4801 gtcggcaccg actgcctcag cttcgatgaa gtccggaaca tcttccgggc cggttccgac
     4861 atcaacagct ggagccaggt cggttacggc cacgatctcg gcggcgacac gaacgaaccg
     4921 aggatgaagg tcgccggacc ggacgaggat tcaaacgtct tcggcttctt cggccagtac
     4981 gtgctcgggg ctccagaacc gaccatgctc gatttccgca gcgactacca ggccttcccg
     5041 accgacgccg gggtgcggcg tgcggtcgta ggcagcaacc gggacttccg ggcggccgcc
     5101 cagttccagc gctcgcggca ggtctaccag gggatcctcg attcgctggc cgagaaccgc
     5161 cgcgcggtgc gtgacgcccg ggccgaagtc gccaagggaa tcgccgacga acgcccgcca
     5221 gaggatcagg cccgcgatcg ccgcaagctg gccaaggccc aggcaaatct cgagaacctc
     5281 gagaagtccc tgcctgcagc ccgccgctat gcgaggcaga atcgcgccgc cctccgtcgc
     5341 ctggcggaag tccgcggaac gctcgggctc ttccgcttct cctactacga ggcattcgaa
     5401 gagcagctgc ggccgatgga agtcagcgca tcgaacgatt tcgcccagcc ggaatgcatc
     5461 ttcccctcgc aggctacggt taccaacgcg acctacccgc tggcccggca actgctcctg
     5521 accatcaatt acaagaacat gactgaccag gacatcaacg ccttcctggt ccagagtctc
     5581 tctcgttccc agcagcttgc tgaagacgca gccctcgtgc cgctgccgga tgagactcag
     5641 gcgaccgagc ttgcctggct gaacggcacc tccgagccgg acataatttt ctacgaatct
     5701 ggcgcaggaa cctcggtcgg aacgcagcaa gaggccgatt cggccagtgg cgagtagccg
     5761 caattcctca cagatccacg cggattttcc gctactcggc cttgaacgcg ggtttaaggc
     5821 ggggtgagat catgattaac gtgtttcggc gtatagcgac aatcctcttg ctcatcggtc
     5881 tgggttttac cttggccgcg atgcctgctg cttccggcgc ggccgcgaaa aagaagcggg
     5941 tcggtccctg ctcgaccttg ccgatcaaca ccaagctgag tacccatgac ccgaccgggc
     6001 tactggagga cggttatgcc tggctgtacc tgaaccccaa gggcaccgtc cgctcgccga
     6061 acgtcaaggt tgtacggcat ggccgcgtgt ttgccagagg ccggatcgtc ggcagtctcg
     6121 taccgggtcg caccacgctg gtcaaaatgc atgttggccg tcgcatgcag cagggcaagt
     6181 atcggatcag ggtgaccgcc agcaagccgg ggcgctgcag caagcgaatc accaagaccc
     6241 gcgtctggaa atttcaccgg cctaccctcg ggctgaaggc gattccggtc agtacccgga
     6301 tcggtgacaa cgtcggcgtc gtccgcttcg ccctgcgacc ggtccgccga accaaggtcg
     6361 gctggaccag ggtttcgctg atcggtcccg gcggtggcgt tgtcagccag caggtgatca
     6421 agtcgatcgg cagcaagcag atcctggccg agctgccgat caaaggcaag ctgaagccgg
     6481 ccaagtaccg ggtccgcctg accgggcagg atctggcttc cggcatcttc cgcgcctcga
     6541 gtcagacctg gcgcttcggc aagggcggcg gtggcgcgaa gccggtgccg accacaggcc
     6601 agctgaccca gaaggtcgcc gtcgactgga ccaacggcaa gtgggacggc cggcaggtcg
     6661 gtggtttcat cgcgcccggc atcggatacg gcgaagtcgt ctgcagcccg taccagcagt
     6721 gggtgcgctt ctacccctct gataccagcc gcgagagcgc gatgatgacc tggacctaca
     6781 agaactggga cttcggcaag ggctacgaaa aggccgtgcg tgaagccaag tacaccgaag
     6841 g
//
LOCUS       DAOQMF010000007         6655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_4819_length_6655_cov_3.479242, whole genome shotgun sequence.
ACCESSION   DAOQMF010000007 DAOQMF010000000
VERSION     DAOQMF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4819_length_6655_cov_3.479242"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLV77_00245"
     CDS             complement(<1..969)
                     /locus_tag="PLV77_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC14310.1"
                     /translation="MRGVAVQGTEEHDAGIRPDEAARKRVAVELIRNHDRPLRWTARR
                     YSICPEDAEDAYQRGIEILLSKAPTTDTRHLLPWTRKVIKHEALAIRRTRERTLARPG
                     QSDIDEHLEDDWVQLIPDISDGPDELTVRRERVARSREALTQLKPQELKALTQLAEGF
                     SYDEIGSMNSWTRTKVNRCLAEGRARFRSTFRESEAGERCPEFEPMISASCDGELTSS
                     QVAELETHLAVCGHCRSTLRAYRAAPRAAAALAPLLPAGRGLWERTQELAVDLQTRLS
                     GVGRGSDSGVQTVLASGGTRGAGMAAIAKIAAICAGTAGTAAVCAAT"
     gene            1255..1479
                     /locus_tag="PLV77_00250"
     CDS             1255..1479
                     /locus_tag="PLV77_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14311.1"
                     /translation="MPSRDEIAATIPRLGRGARINLKLKYGGEVLGTLYGLFDDQVFF
                     EEEEIAPIDLDRIDDLLVRVHTEQPGGPAS"
     gene            complement(1614..1814)
                     /locus_tag="PLV77_00255"
     CDS             complement(1614..1814)
                     /locus_tag="PLV77_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14312.1"
                     /translation="MSGGGWRYSSGIVSQFGRYCRDCLHEKGVKTTSYSAAIEKLPSD
                     KQVEVEKLRNKWTVEDARRKPE"
     regulatory      complement(2486..2585)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2599..2910)
                     /locus_tag="PLV77_00260"
     CDS             complement(2599..2910)
                     /locus_tag="PLV77_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14313.1"
                     /translation="MDKGEPEGCLCVRTRVREDLENLCQLESLTSYADTIEESRFSDY
                     RFRIHVKREDWVKAAAVLAEQIDYDNFKNAVKARQGRERAGVYTQVWNDLYGMQEPFA
                     R"
     gene            3122..4486
                     /locus_tag="PLV77_00265"
     CDS             3122..4486
                     /locus_tag="PLV77_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNC14314.1"
                     /translation="MPFAQAAKVPAGAGSGAVAPIAHKGRWFVDATGRVVQLRGVNEV
                     YKTAPYYPAADGFGADDVNLLSKLGLNVVRLGVDLRGLMPTPGKIETAYIDKLAQTVN
                     QLTKAGIYTQVDIHQDGYAPKYNGNGFPDWMAIDDGLPNPDLPFPVYYFGNTAMQRAW
                     DHFWDNSEVNGKGLQEYVIQSLTALVKKFRGNPHVVGYDVFNEPWPGTDFAPCAEATG
                     CPQLERELMMPFARKAVKATRKLTKTQPVFVEPFLFFNLGRPTVLPGSTGAWLSAHNY
                     ATDENDALMVKETVKAATRDQVPAILTEFGATSDNATIDRLTSIYDARIVSWIFWAYN
                     ENVVPDGSVTAGTGTATQDVIQALARPYPVAVTGTPTSLKFDPDTRVMDLTYGTAKRG
                     GGKFPAPLPSVVSVPRYTYPTGYKAIVKGARVTSAACSPKLRLQTRPGARKVSLTVKP
                     ASSC"
     gene            complement(4529..4933)
                     /locus_tag="PLV77_00270"
     CDS             complement(4529..4933)
                     /locus_tag="PLV77_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNC14315.1"
                     /translation="MIVADTSVWVDYLNGVWAPGSRELRRLLEEDETVCMTDHVLSEI
                     LQGYRREAEADEIASLLLTFPFLGLEGPGDYLAAASVYRKARSAGITLRSTVDLLIAI
                     PCIRREAFLLHNDRDFDQLARVSDLKIWQPAG"
     gene            complement(4930..5175)
                     /locus_tag="PLV77_00275"
     CDS             complement(4930..5175)
                     /locus_tag="PLV77_00275"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNC14316.1"
                     /translation="MRKGQAMCIVMCMRTNIVIDDELMAEAQKLTGTKTKKETVDLAL
                     REMVAHRRRQGILELPGKFKWEGDLNEMRRNFFRKFD"
     gene            complement(5232..6149)
                     /locus_tag="PLV77_00280"
     CDS             complement(5232..6149)
                     /locus_tag="PLV77_00280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNC14317.1"
                     /translation="MKLKTGVAALAVSMLLFTADAGAAVSHQVTGPEAKASRGFWTAA
                     RVAAALKRDPDSALNAASSPDRHPVATHHRPVTVYRPEVGKILAWDRNIGEFSCSGSI
                     IDTRSLRLVLTAGHCVYADGVWARRHIFIPNFKNGRRPFGAYRVKATWVSNAWFNNSF
                     GTRGMNFDIGLLVTRKRWNGSRIGENVGAIPIQTFPRRYGVTDIYGYPGGAMRTRVMR
                     TCRSHTKADWYGGRFFYGPTGLLARCNMAAGSSGGPWVSRYKAEGGGTIGVVDGLTST
                     GFSQGGRSYLTSPYFGRILVRLIKATEGR"
     gene            6272..6505
                     /locus_tag="PLV77_00285"
     CDS             6272..6505
                     /locus_tag="PLV77_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14318.1"
                     /translation="MARTEGQIPDGLLTGMERQAAIRGVDRDEMFQSAVRSELATYRN
                     REPGPLVDLDELRLSFDWPASDPDVGRRGRRHL"
BASE COUNT         1219 a         2194 c         2002 g         1240 t
ORIGIN      
        1 ggtggcggca caaaccgccg cggtacccgc tgtgccggcg cagatcgccg cgatcttggc
       61 gattgccgcc atcccggcac cacgagtacc tcccgaggcc aggaccgtct gaactccgga
      121 gtccgaaccc cggccgactc ccgagaggcg agtctgcagg tccaccgcga gttcctgggt
      181 tcgctcccag agcccccggc cggccggcaa caggggagcc agcgctgcgg ccgccctggg
      241 ggcagcgcga taggcgcgaa gggttgaccg acagtggccg cagacggcga ggtgggtttc
      301 cagctcggca acctgggatg aggtcaactc cccgtcgcag ctggccgaga tcattggctc
      361 gaattcgggg cacctctctc ccgcttcgct ttcccggaag gtacttcgga aacgagcccg
      421 cccttcggcc aggcaccggt tcaccttggt gcgggtccac gagttcatcg acccgatttc
      481 gtcgtatgag aatccctctg cgagttgagt cagggccttc agttcctggg gcttcagctg
      541 agtcagggct tcccggctgc gggccaccct ctcgcggcga acggtgagtt catccgggcc
      601 gtcgctgata tccgggatga gctgcaccca gtcgtcttca agatgctcgt caatgtcgga
      661 ctggcccggg cgggcgagcg tcctctcgcg ggtccgccga atcgccaaag cctcgtgctt
      721 gatgaccttc ctcgtccagg gcaggagatg ccgcgtgtcg gttgtcggcg ctttcgagag
      781 aaggatctcg atgcctcgct gataggcgtc ttcggcgtcc tcgggacaga tcgagtagcg
      841 gcgtgccgtc cacctgagcg ggcgatcgtg attcctgatc aactcgaccg caacccgctt
      901 gcgcgccgcc tcgtccggcc tgatcccggc gtcatgctct tccgtgcctt gaaccgcaac
      961 tcccctcatg gggtccccca ctccttagac ggcttggcac tcttcctatc cgggagaccc
     1021 gaaaattgcc agcggcagaa atctcgccca cggaaccttg cccctacatt ttcaaaccat
     1081 cgaaaagcgt ccatttgcag cgataagggc cgcaggttcg aaaactctcc ccccttcaaa
     1141 gtccgatttc tccgcaaatt gcgaactatt cggaatttca tgcaaattgc atggaatttc
     1201 tgattggtct cagttcggaa ctcgcaaatt gggtcttctg gctaggctcc acccatgccc
     1261 tcgagagacg agatcgccgc gacaatcccc cgcctcggcc ggggcgcccg gatcaacctg
     1321 aagctgaaat acggcggcga agtgctcggc accctctacg gcctcttcga cgatcaggtc
     1381 ttcttcgaag aggaagaaat cgcgccaatc gacctcgacc ggatcgacga cctcctggtc
     1441 cgggtccaca ccgaacaacc cggtgggccg gcctcgtgag agttcgatgt ccaaggtgag
     1501 tgggtacacg ccaatcgacg ttcccgaacc gaacgagtcc ttccgttccg gcagcagtcc
     1561 gttcggcacg cctcgagaat gaccggctcc tcaagacaca gccgcccgcc tgactattcc
     1621 ggcttcctcc gggcatcctc gaccgtccac ttattgcgaa gcttctcgac ctcgacctgt
     1681 ttgtcggatg gaagcttctc gattgccgcc gagtagctcg tggttttgac ccctttctcg
     1741 tggagacaat cccggcagta gcggccgaac tgggagacga tgccggagga gtagcgccag
     1801 ccgccaccgc tcaccgagta acagcaggtc tgagcttcac cggtggtgga gcgggcaccg
     1861 caggggcatc gggcgatttc acatcgacaa cgggcagatc ttctgtcggg gtttccgtcg
     1921 tagccagccg aataagtgaa tccggttccc caattacagg tgggacattc acgaagtccg
     1981 atcggaatcc atcctccggg cggctcgatt gcgacagcga cgtcattctc cgtggggaca
     2041 ttgagaggca gactcctcca ggcaccgcaa aacgtaccgt gttgcgcacc tcgccatcgg
     2101 gcgagtttct ccaggacctg cccctcacct cgatacccga gccgcaagcc ctcacggcag
     2161 tcagagcaca accagctaag cccagcgcgg ttttcaggtg tagccgcggt ttcgcagcga
     2221 cagacttcgc aaaatctcca gccagtgaag atccctctgc aggctcgcgt gaagcacgac
     2281 tggtgctcat cccagaaggc gatgaagtcg ccccgctgct cgtaagcggc gatctcgtgt
     2341 tcgatcgaag gcaatctcgc tgcctcgccg catgactgac atattcgtcc ctgcgggccc
     2401 tcgggaactc gcttcgtgta ctcgtccaga atcgcatcaa ttgcgctgaa ctcggcgtcg
     2461 atctctgatt ggtacggcat tcttacctcc atcgattccg gctttagcac cgtttccggg
     2521 ggttgaatcc cgactggttg ctgcggcgtc acggggccgg ttccctcgac cgctcttgat
     2581 agatgtctgc tgctggtctt acctggcgaa cggctcctgc attccgtaaa ggtcgttcca
     2641 gacctgggtg tacacgccgg cccgttctct tccctggcga gctttgaccg cgttcttgaa
     2701 gttgtcgtag tcgatctgtt cggccaggac ggctgcggcc ttgacccagt cctcccgctt
     2761 cacgtggatc cggaagcggt agtcactgaa cctgctttcc tcgatcgtgt cggcgtacga
     2821 ggtgagggac tcgagctggc agagattctc aaggtcctcg cggacccggg tccgtacaca
     2881 caggcagccc tcgggctcgc ccttgtccac cactgaaaag aaaccacggt cagtcactat
     2941 ccacatcggc accccctcgt ttgctggctg gcacatggag agtcgcccgg gaaccggatt
     3001 tctgacaaag gcccttgcca aaaagactga gctacttccg ggctcgacta ctgtttgcac
     3061 tctcacgaac ccgattcgcg ctggtctagc caccctggcc gtagccgcat cgctcgtcgc
     3121 ggtgccgttc gcacaggccg ccaaggttcc cgccggggca ggttccggcg cggttgcccc
     3181 gatcgctcac aaaggccgct ggttcgtgga tgcgaccggg cgggtcgtgc agctccgcgg
     3241 ggtcaacgag gtctacaaga cggcgccgta ttacccggcg gcagacggat tcggagccga
     3301 tgacgtcaac ctgctgagca agctcggact caacgtggtt cggctggggg tcgacctgcg
     3361 tggcctcatg ccgacccccg gcaagatcga gaccgcctac atcgacaagc tggcgcagac
     3421 ggtcaaccag ttgaccaagg ccgggatcta cacccaggtc gacatccacc aggacggtta
     3481 cgcccccaaa tacaacggca acggctttcc cgactggatg gcaatcgacg acggcctgcc
     3541 gaacccggat ctgccattcc ccgtctacta cttcggcaac accgccatgc agcgggcctg
     3601 ggatcacttc tgggacaact cggaggtcaa cgggaagggc ctgcaggaat acgtgatcca
     3661 gagccttacc gccctggtca agaagttccg gggcaacccc catgtcgtcg gttacgacgt
     3721 cttcaacgag ccgtggcccg ggaccgactt cgcgccctgc gccgaagcaa ccggctgccc
     3781 gcaactcgag cgcgaactga tgatgccctt cgcccgcaag gcagtcaagg cgacccgcaa
     3841 gttgacgaag acccagccgg tgtttgtcga gccgttcctc ttcttcaacc tcggccgtcc
     3901 gaccgttttg ccggggtcaa ccggcgcctg gctatcggcc cacaactatg cgaccgacga
     3961 aaacgacgcc ctgatggtta aggagacggt caaggcggcc acccgggacc aggttccggc
     4021 gatcctgacc gagttcggcg cgaccagcga taacgcgacg atcgaccggc tgaccagcat
     4081 ctacgatgcc cggatcgtct cctggatctt ctgggcctac aacgagaacg tggtacccga
     4141 cggatcggta accgcaggca ccggcaccgc cacccaggac gtaatccagg cgctggcccg
     4201 cccctatcca gtggccgtca ccggcacgcc cacctcactg aagttcgatc cggacacccg
     4261 ggtgatggac ctgacctacg gcacggcaaa gcgtggaggg ggcaaattcc ccgccccgct
     4321 tcccagcgtt gtctcggtac cccgctacac ctacccgacc gggtacaagg ccatcgtcaa
     4381 gggggccagg gtcacctccg ctgcctgctc cccgaagttg agactgcaga ccaggcccgg
     4441 cgcccgaaag gtcagcctca cggtgaagcc cgccagctcc tgctgacggc aaagctcaac
     4501 gggctaccta tcccggtgcc ggcccgggtc agccggccgg ctgccagatc ttcaggtccg
     4561 agaccctggc aagttggtcg aagtcgcgat cgttgtgcag gaggaaggct tcacgacgaa
     4621 tgcaaggaat ggcgatcagg agatcgaccg tgctgcggag ggttattccc gcagaccggg
     4681 cctttcgata gacgctcgct gcggccaggt aatcgcctgg accctccagg cccagaaagg
     4741 ggaaggtgag caagaggctt gcgatttcat ccgcttccgc ctccctgcgg tagccctgaa
     4801 gaatctcaga gagaacgtga tcggtcatgc acacggtttc gtcctcctcg aggagccgac
     4861 ggagctctct cgaaccgggt gcccagacac cgttcagata gtcgacccag acgctcgtgt
     4921 cagcgacaat caatcgaact tccggaagaa attccgacgc atctcgttca ggtcgccttc
     4981 ccacttgaac ttgcccggca actccaggat cccttgccgg cggcggtggg ccaccatttc
     5041 ccgcagggcg agatcgactg tttccttctt ggtctttgtc ccggtcagct tctgggcttc
     5101 ggccatcagt tcgtcgtcga tgacgatgtt ggtcctcata cacatcacga tacacatcgc
     5161 ctgacccttt ctcaacggat cgctccattg aggaggtaag tgccgggggc cggggattcg
     5221 ccccccgggg gtcagcggcc ttcggtggct ttgatcaggc ggaccaggat ccggccgaag
     5281 taggggctgg tcaagtagga cctgccgccc tgggagaagc cggtgctggt cagaccgtcg
     5341 accactccta tcgtgccgcc tccttcggcc ttgtaacggg aaacccaggg gccgccgctg
     5401 gagccggccg ccatgttgca gcgggccaga aggccggtcg gtccgtagaa gaaccggcct
     5461 ccgtaccagt ccgccttggt gtgggaacgg caagtacgca tcacccgggt ccgcatcgcc
     5521 ccgcccgggt acccgtagat gtcggtgacc ccgtatcggc ggggaaaggt ttggatcggg
     5581 atcgccccga cgttctctcc gatccgcgag ccgttccacc gtttgcgggt gaccagcagt
     5641 ccgatgtcga aattcatgcc ccgggtcccg aaggaattgt tgaaccaggc attggacacc
     5701 caggtggcct tgacccggta ggccccgaac ggacggcggc cgttcttgaa gttcgggatg
     5761 aagatgtgcc tccgcgccca gaccccgtcg gcatagacgc aatggcccgc agtgaggacg
     5821 agccgcaggc tgcgggtgtc gatgatactg ccgctgcagc tgaactcgcc gatgtttcga
     5881 tcccaggcga ggatcttgcc gacctcgggc cggtacaccg tgaccggacg gtgatgggtt
     5941 gcgaccgggt gccggtcagg cgaggaggcg gcgttcagag cggagtccgg gtcacgcttg
     6001 agcgcggcag ccacccgggc cgctgtccag aaaccccggg atgccttcgc ctccggcccg
     6061 gtcacctggt ggctgaccgc tgcgccggca tcggcggtga acagaagcat cgagacagcg
     6121 agagctgcga ctcccgtttt cagtttcatg ccccccatgt cgcttccatt ctccctgatc
     6181 tgacaaagga attccgcaaa tgccgggttt ttcatcgacc cggggttgac atatctcaca
     6241 caggccgaag gaccggcgct atgttcggcc aatggcaagg acggagggac agatcccgga
     6301 cggactgctc acaggaatgg agaggcaagc cgcgatccgc ggggttgacc gggacgaaat
     6361 gttccagtcg gccgtgcgaa gcgagctggc cacctaccgg aaccgtgagc ccggtccact
     6421 ggttgatctg gatgaactcc gactgagctt cgactggccg gcgtccgacc cagatgttgg
     6481 gcgccgcggg cggcggcatc tctgaagctg gccggcgcgc ccctcgcggg gcgcccgacc
     6541 gggccgccgg ggatgccgcc gcgattcagt cgattgactg atcgaccaga acctcgaata
     6601 cgccgctggt tcctgtcgat tcgatgaacg gggccaccgc gttcatcgaa tcgac
//
LOCUS       DAOQMF010000008         6553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_4942_length_6553_cov_4.098954, whole genome shotgun sequence.
ACCESSION   DAOQMF010000008 DAOQMF010000000
VERSION     DAOQMF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4942_length_6553_cov_4.098954"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PLV77_00290"
     CDS             complement(<1..924)
                     /locus_tag="PLV77_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC14319.1"
                     /translation="MTSTPEQVQRVYLTLMLGNTLAASFIWGINTIFLLDAGLSNLEA
                     FAANAFFTAGMVIFEIPTGVVADTIGRRVSYMAGTLTLMATTLLYVMLWEINGPFWAW
                     AVISLLLGLGFTFFSGAVDAWLVDALDETGFTGDLERVFGRGMTVTGIGMLAGSVIGG
                     YVAQVTSLGVPFVMRAVVLLAMFFFAAWAMKDLGFKPHKAESTFGEMKSITRNSIDYG
                     WRVPSVKWIMLAAPFTAGVGIYAFYALQPYLLELVGDPHAYGIAGITAALVAGSQIIG
                     GFLAPRIRRRFRLRTSALLLAVSVGAVTLALI"
     gene            966..1418
                     /locus_tag="PLV77_00295"
     CDS             966..1418
                     /locus_tag="PLV77_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNC14320.1"
                     /translation="MTKVRRFLVVLLVLALGAGVAACGSDSDSGSGEGSEASAVEGVT
                     WQAMNLVTQGAATSLPKGVKPPTLEINDGQASIFAGCNSGSGEATVNEATIDFGPIAL
                     TRKSCEQVQNQIEFLVTQVLQGEVKYEVNSDGNLILEKGTDSIVYTKG"
     gene            1537..3048
                     /locus_tag="PLV77_00300"
     CDS             1537..3048
                     /locus_tag="PLV77_00300"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNC14321.1"
                     /translation="MTVSTETETTTLQNYVGGKWVEAGGAELHDVVNPANGEVIARVP
                     FSTASHLDEAVRAARKALPAWRGVSTIARARMLFTLREKLEARKDEIARCVVTEMGKT
                     LPDATAEVGRMIEMVEAATAIPTTMQGKVLEDVARGVDAETIRQPVGVCGIIAPFNFP
                     AMVPFWFLPFAIACGNTIVLKPSEQVPMTQQKVFGIIDELDLPDGVVNLVNGSREVVE
                     GMLDHPDIDAISFVGSAPVAQLVYERAAQTGKRVQALGGAKNHMVICPDAVVEPTVNG
                     LIGSGFGAAGQRCMAGSVIVTVGSGHDSVVPKLVEETKKLHVGNGLDENIDVGPVISC
                     DARDRIDGWVARAIEEGAEVLVDGRTPLPDAPEGSSYVGPTILDNVKPTDSIIENEVF
                     GPVIAIVNVDTLEEAIEVVNASPFGNGVSIFTESASAVRKFRHDVEVGMVGVNIGVAA
                     PVAFFPFSGWKASFLGDLHAHGPDGVEFFTRKKTITSRYFSGGHESGSYFVER"
     gene            complement(3045..4193)
                     /locus_tag="PLV77_00305"
     CDS             complement(3045..4193)
                     /locus_tag="PLV77_00305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC14322.1"
                     /translation="MAKRATKRGDERTDLTGSRDVLICGASFAGLMVARQLAGTGADV
                     LVVDRYEIGERQTSACAIPTAWLEELGLIESEKQRFDSVLIHTAHGSIRLPVPWSFST
                     FDYPSFCEMLWAQCDAEFETAKVNGRAKTPEGDHVIDTDRGEITAPLVVDSLGWKRVL
                     AAGAHYQPPNQPLSRGLEVHPPGGNVDLEVFINRRYVPAGYGWSFPADDEVRVGVGSF
                     DPHFPVRPTTDLIADDLNVEKSAYQGNWIPHKLRPATGDGIFFAGDSAGHCFPLTAEG
                     IRPALYFGAALGMELRDVVEGRKSREEALTCYHDFSAGHQWAFDWLLRAQKLLPKVHP
                     RLVKPTLAASFKAGGSGDYAFNKYLDIAPPEFAALATRALKENSPVPT"
     gene            4336..5868
                     /gene="rpsA"
                     /locus_tag="PLV77_00310"
     CDS             4336..5868
                     /gene="rpsA"
                     /locus_tag="PLV77_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNC14323.1"
                     /translation="MTTTETTLQPAPEGVPVEGSNGLLINVDGRIIPNYAATIVSFEE
                     GDVVNGKVVRIDRDEVLLDIGYKSEGVIPAGELSIRKNVDTSEEVELGEEIDALVVTK
                     EDSEGRLILSKKRARFEKAWRKIEAAAENGEPVEGNVIEVVKGGLILDLGIRGFLPAS
                     LVDIRRVHNLDEFMSTTLECKVIELNRSRNNVVLSRRAVLEEERKEVRDQILDRLQPG
                     EVVEGKISNIVDFGAFVDLEGIDGLIHISELSWSHVNHPSEVVAIGDTVKIKVLDIDR
                     DRQRISLGLKQTQEDPWQRVVSTYRSGDVLEGTVTKVVSFGAFVEILDGVEGLVHISE
                     LADHHVENPSEVVELGASLNVKILEIDEERRRLSLSIKRVEGQNMLLKDLGAALAAAS
                     GEPAVEEETPELGLSEEVFADDAEAPADSAEEVAPEVEAVVDEAIAEAEVADAAEQAE
                     AADETPEEVVEDAVAEAEIEAAADEAAAEEAVVEEAGEITEEAAAEEAAEGEAPEGEE
                     QS"
     gene            5877..6461
                     /gene="coaE"
                     /locus_tag="PLV77_00315"
     CDS             5877..6461
                     /gene="coaE"
                     /locus_tag="PLV77_00315"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNC14324.1"
                     /translation="MTGGVAAGKSEALSAFGRLGAATISADQVVHDLLDREPLLGRLK
                     ERWGDEVAPEGRVDRGVVGAKVFNDPEELAWLESQIHPLVGEEFRGWIEGLDPATKFA
                     VAEIPLLFEGEMYRAFDHTVAIVAHEDTRQERARSRGHEGVEGREARQLSQAEKAERA
                     DTVIENDGTPAELESKLATLLEKFESELATGGQS"
BASE COUNT         1273 a         2056 c         2171 g         1053 t
ORIGIN      
        1 gatcagggcg agcgtcaccg caccgacaga gaccgccagg agcagcgcgg atgtccgcaa
       61 cctgaaacgg cggcggatcc tcggggcgag gaagccgccg atgatctggg agcccgctac
      121 cagcgcagcg gtgatgccgg cgatgccgta ggcatgcggg tctccgacca gctccagcag
      181 gtatggctgc agcgcgtaga aggcataaat cccgaccccg gcggtgaagg gagccgcaag
      241 catgatccac ttgaccgaag ggacccgcca gccgtaatcg atcgaattcc gggtgatcga
      301 cttcatttcg ccgaaggtgc tttcggcctt gtgcggcttg aaaccgaggt ccttcatcgc
      361 ccatgcggca aaaaagaaca tcgccagcag aacgaccgct ctcatcacga acggcacccc
      421 cagactggtc acctgggcca cgtagccgcc gatcaccgag ccggccagca tcccgattcc
      481 ggtcacggtc atgccccggc caaagacccg ttccaggtct cccgtgaacc ccgtttcgtc
      541 cagtgcgtca accagccagg catcgacggc gccggagaag aaagtgaaac cgagcccgag
      601 cagcagtgat atgaccgccc aggcccagaa gggtccgttg atctcccaga gcatcacgta
      661 gagcagcgtg gtggccatca gggtcagggt cccggccatg taggagaccc ggcggccgat
      721 cgtgtcggcg acgaccccgg tcggaatctc gaagatcacc atgccggccg tgaagaaggc
      781 gttggcggcg aaggcctcca ggttggaaag cccggcatcc agaaggaaga tcgtgtttat
      841 cccccagatg aacgaggcag caagcgtgtt gcccagcatc agggtcaggt agacccgctg
      901 aacctgctcc ggcgtggagg tcacgttggg accgtaccca tcacgatcgg atataccctt
      961 cagtcatgac caaggtgagg agatttctgg tggttctgtt ggttctggcg ctgggtgccg
     1021 gggtagcggc ctgcgggtcg gactccgatt cagggtcggg cgagggctcc gaggcttccg
     1081 cggtcgaggg cgtgacctgg caggcgatga acctcgtaac ccagggcgcc gcgaccagcc
     1141 tgcccaaggg agtcaagcct ccgacccttg agatcaacga cggccaggcc agcatcttcg
     1201 cgggctgcaa cagcggctcg ggcgaagcga cggtaaacga ggccacgatc gacttcgggc
     1261 cgatcgccct gaccaggaag tcctgcgaac aggtccagaa ccagatcgag ttcctggtca
     1321 cccaggtgct tcagggcgag gtcaagtacg aggtcaacag cgacggcaac ctgatcctgg
     1381 aaaagggcac cgacagcatc gtctacacga agggctgacc cggccaggtt ctggacgtgg
     1441 cgtccagttt ggcgttgaga cttcacggta tgtccacttt cggtctggtc tccgggcgtt
     1501 agcctgagaa agacccgttt tccccgagga ggccgtatga ccgtcagcac cgagaccgaa
     1561 accaccacgc ttcagaatta cgttggcggc aagtgggtcg aagccggcgg ggccgagctt
     1621 cacgacgtgg tcaatccggc caacggagag gtcatcgccc gggttccctt ctcgaccgcc
     1681 agtcatctcg acgaagcggt tcgagccgcc cggaaggctc ttccggcgtg gcgcggcgta
     1741 agcacgatcg cccgggcccg gatgctcttc accctgcgcg agaagctcga ggcgcgcaag
     1801 gacgaaatcg ctcgctgcgt ggtcaccgag atgggcaaga ccctgcccga tgccaccgcc
     1861 gaagtcggcc ggatgatcga gatggtcgag gcggccaccg cgatcccgac cacgatgcag
     1921 ggcaaagtgc tcgaggacgt ggcccgcggc gtggacgcgg aaacgatccg ccagccggtc
     1981 ggggtctgcg gcatcatcgc tcccttcaat ttcccggcca tggtgccgtt ctggtttttg
     2041 cccttcgcga tcgcctgcgg caacacgatc gtgctgaagc cttcggagca ggtgccgatg
     2101 acccagcaga aggtcttcgg aatcatcgac gagctcgacc ttccggacgg ggtggtcaac
     2161 ctcgtaaacg gcagccgcga ggtcgtcgag ggaatgctcg atcaccccga catcgacgcg
     2221 atctccttcg ttggatcggc cccggtcgcc cagctcgtct acgagcgcgc ggcccagacc
     2281 ggcaagcgcg tccaggctct cggcggggcc aagaaccaca tggtgatctg ccccgacgcg
     2341 gtggtggaac cgaccgtcaa cggactgatc ggctccggct tcggcgccgc cggtcagcgc
     2401 tgcatggccg gctcggtgat cgtcaccgtg ggctcgggcc atgacagcgt ggttccgaag
     2461 ctggtcgagg aaacgaagaa gctccatgtc ggcaacgggc tcgacgagaa catcgacgtc
     2521 ggcccggtca tttcctgcga cgcccgcgac cgcatcgacg gctgggtcgc cagggcgatc
     2581 gaggaagggg cggaggtcct ggtcgacggg cgcacgccgc tgcccgatgc ccccgagggc
     2641 tcgtcctacg tggggccgac aatcctcgac aacgtcaaac cgaccgattc gatcatcgag
     2701 aacgaggtct tcggcccggt catcgcgatc gtcaacgtcg acactctcga ggaggcgatc
     2761 gaggtcgtca acgccagtcc cttcggcaac ggcgtttcga tcttcaccga atccgcctcg
     2821 gcggtccgca agttccgcca cgacgtcgag gtcggcatgg tcggcgtcaa catcggcgtg
     2881 gctgctccgg ttgccttctt ccccttctcg ggctggaagg catccttcct cggtgacctc
     2941 cacgcccacg gccccgacgg agtcgagttc ttcacccgta agaagacgat cacgagccgc
     3001 tatttctcgg gcggccacga atccggctcc tacttcgtag aacgctaggt gggtacgggc
     3061 gagttttctt tgagggccct tgtggcgagg gcggcgaatt ccggtggggc gatgtcgagg
     3121 tacttgttga aggcgtagtc acccgagccg ccggccttga aagaggccgc gagggtcggt
     3181 ttgaccaggc gggggtggac cttcggcagg agcttctggg ccctgaggag ccagtcaaag
     3241 gcccactggt ggccggcgct gaagtcgtgg tagcaggtca gggcctcttc gcgtgacttg
     3301 cggccttcca ctacatcgcg aagttccatg ccgagcgcgg caccgaaata gagggcgggg
     3361 cggatgccct cggcggtcag ggggaagcag tggccggccg agtcaccggc gaagaagatg
     3421 ccgtcgccgg tcgcgggccg gagtttgtgg gggatccagt tgccctggta tgccgacttt
     3481 tcgacgttga ggtcgtcggc gatcaagtcg gtggtcgggc ggaccgggaa gtgaggatcg
     3541 aaggaaccga ccccgacccg gacttcgtcg tcggccggga aagaccagcc ataaccggcc
     3601 gggacatagc gccggttgat gaaaacttcg aggtcaacgt tgccacccgg cgggtggacc
     3661 tccaggccgc gagaaagcgg ctggttgggc ggctgatagt gggcgccggc agccagaacc
     3721 cgcttccagc cgagcgaatc gaccaccagc ggagcggtta tttcgccccg atcggtgtcg
     3781 atcacatggt ctccctcagg ggtcttggcc ctgccgttga ccttggcggt ttcgaactcg
     3841 gcgtcgcact gggcccagag catttcgcaa aagctcgggt agtcaaaggt ggagaaggac
     3901 caggggaccg gcaagcggat cgaaccgtgg gcggtgtgga tgaggaccga gtcaaatcgc
     3961 tgcttctcgg attcgatcag ccccagctcc tccagccagg cagtcgggat cgcacaggcg
     4021 gaggtctggc gctcgccgat ctcgtagcga tcgacgacca ggacgtccgc gccggtaccg
     4081 gccagctggc gggccaccat cagtccggca aaactggctc cacagatgag aacgtcgcgc
     4141 gatccggtca gatcggttcg ttcgtccccc cgctttgtcg cccgttttgc catccggtgg
     4201 agggtaatga aaaccggggc acagaagtca gagcccctcc caaccatcag tttgttcgaa
     4261 tgggaggggc gggttgaaca tagagggcgg gtcggacccc gcccgactcc tgatatctgc
     4321 catcatcccc ggactgtgac tactactgaa accacactcc aacccgcccc agagggggtt
     4381 ccggtcgaag gctcaaacgg cctgctgatc aacgttgacg gccggatcat tcctaactac
     4441 gcagcgacga tcgtttcgtt cgaggaaggc gacgtcgtaa acggcaaagt cgtccgcatc
     4501 gatcgcgacg aagtcctgct cgacatcggc tacaagtcgg aaggtgtaat tccggccggc
     4561 gagctctcga ttcgcaagaa cgtcgacacc tcggaagagg tcgaactcgg cgaagagatc
     4621 gacgccctgg tcgtcaccaa ggaagattcc gaaggccggt tgatcctttc caagaagcgt
     4681 gcccgcttcg agaaggcctg gcgcaagatc gaagctgccg ccgaaaacgg cgagccggtc
     4741 gaaggcaacg tgatcgaggt cgtcaagggc ggcctgatcc tcgacctcgg catccgcggc
     4801 ttcctgcctg cctcgctggt tgacatccgc cgggtccaca acctcgacga gttcatgtcg
     4861 accacgctcg agtgcaaggt catcgagctc aaccgcagcc gcaacaacgt cgtgctttcc
     4921 cgccgcgcag tcctcgaaga ggagcgcaag gaagtccgcg accagatcct cgatcgcctg
     4981 cagccgggcg aagtcgtcga aggcaagatc tcgaacatcg tcgacttcgg cgctttcgtg
     5041 gacctcgaag gcatcgacgg cctgattcac atctccgagc tttcctggag ccacgtcaat
     5101 cacccctccg aggtggtggc gatcggcgac acggtcaaga tcaaggtgct cgacatcgac
     5161 cgcgaccgcc agcgcatctc gctgggtctc aagcagaccc aggaggaccc gtggcagcgg
     5221 gtcgtctcga cctaccgttc gggcgacgtg ctcgagggca cggtcaccaa ggtcgtctcc
     5281 ttcggtgctt tcgtcgagat cctcgacggc gtcgaaggcc tggtccacat ttcggaactg
     5341 gccgaccatc acgtcgagaa cccgtcggaa gtcgtcgagc tcggcgccag cctcaacgtc
     5401 aagatcctcg agatcgacga agagcgtcgc cgcctctcgc tttcgatcaa gcgggtcgag
     5461 ggccagaaca tgctgctcaa ggacctgggc gccgccctgg ccgccgcctc cggcgagccg
     5521 gccgtcgagg aagaaacccc ggaactgggt ctctcggaag aggtctttgc cgatgacgcg
     5581 gaagccccgg ccgatagtgc cgaagaggtc gctcccgagg tcgaggccgt agtcgacgag
     5641 gcgatcgccg aggccgaagt ggccgacgcc gccgagcagg ccgaggccgc ggacgaaacc
     5701 ccggaagagg tcgtcgagga cgccgtcgcc gaggccgaga tcgaagctgc cgctgacgaa
     5761 gccgctgccg aggaggccgt ggtcgaggag gccggtgaga tcaccgaaga agccgccgcg
     5821 gaagaggcag ccgaaggtga agctcccgaa ggggaagaac agtcctgacg atcggactga
     5881 ccgggggcgt agctgccggt aagtccgagg cactgagcgc ctttgggcgg ctcggcgcgg
     5941 ccacaatctc tgccgatcag gtcgttcacg acctgcttga ccgggagccg ctgctgggtc
     6001 gcctcaaaga gcgctgggga gacgaggttg ccccggaagg ccgcgtggat cgcggggtgg
     6061 tcggggcaaa ggtcttcaac gacccggaag agctggcctg gctcgaatcg cagatccatc
     6121 cgctggtcgg cgaggaattc cgcggctgga tcgagggtct cgatccagcg acgaagttcg
     6181 cggtggccga aatcccgctc ctcttcgagg gtgagatgta ccgcgccttc gatcacaccg
     6241 tcgcgatcgt ggcgcatgaa gacacccgcc aggaacgggc ccggtcgcgc gggcatgaag
     6301 gggtcgaggg ccgggaggcc cggcagctga gccaggctga aaaggccgag cgggcagaca
     6361 cggtcatcga aaatgacgga accccggccg aactggaatc gaagctggcg accctgctcg
     6421 aaaagttcga gtcagagttg gcgaccggcg ggcaaagcta ggctataagg ccacctcgca
     6481 tggcccagaa ccgaacttca acagcgggcg gtcgacgccg ctcaaacccg gccccccggc
     6541 gcggcagcac gaa
//
LOCUS       DAOQMF010000009         6552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_4947_length_6552_cov_3.470371, whole genome shotgun sequence.
ACCESSION   DAOQMF010000009 DAOQMF010000000
VERSION     DAOQMF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4947_length_6552_cov_3.470371"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLV77_00320"
     CDS             complement(<1..1025)
                     /locus_tag="PLV77_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC14325.1"
                     /translation="MIEEEIEEPTGSQVLVSVEATPVNPSDLGVLIGPADASTLGPAE
                     GGGLNGKVPEQALPIMRDRLDKPLPVGNEGAGTVVAAGPEAADLVGRTVSLVGGAMWS
                     DYRLVDGAAVIPLPDDATPADGAALFVNPMTALSMVETMRAEGHTALVHTAAASNLGQ
                     MLVKICKDDEVGLVNIVRKPEQAATLEALGAEHVVDSSLPDFDQRLVEAISATGATLA
                     FDAVGGGTLASDILSAMERSQPPAEFWTPYGSAVHKQVYTYGSLDFGPTIIQRTFGMR
                     WSVGGYLLTDALGRLGGETVMAMRDRVVSEIKTTFSSSYAETIGLADILDPVHLAAAG
                     KRGTGAKL"
     gene            complement(1152..1547)
                     /locus_tag="PLV77_00325"
     CDS             complement(1152..1547)
                     /locus_tag="PLV77_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC14326.1"
                     /translation="MLSLHHTHLMASDIDETIRFWAEGFGGEVVFDREFAGARNVFMT
                     VGKGRIHLYDQAPKVSGQAVVHHLGVQTDEIETVVARLRSMDISVTDVRTEPDASYAM
                     ARGPDDLLVEIFQPDPEAVPEELRPYFGL"
     gene            1638..1991
                     /locus_tag="PLV77_00330"
     CDS             1638..1991
                     /locus_tag="PLV77_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC14327.1"
                     /translation="MSPELTLTPKQKSPNGQPCCEPVVYPDVDRARAERMAKVAKALG
                     DPIRLTLVDVLRKHAGKVCVCELVPLFDVNQSTLSHHLKKLRDAGIVDSERQGLWSYY
                     YVNPEELEELNAWLS"
     gene            1979..2857
                     /gene="arsM"
                     /locus_tag="PLV77_00335"
     CDS             1979..2857
                     /gene="arsM"
                     /locus_tag="PLV77_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNC14328.1"
                     /translation="MAELEKTTDCCSPEDQATCCEPEAKSECCGEGPECGCPDGAERT
                     DSELREIVRERYAAAARDAGSRDASCCGNDAVITEDQRAVFGSSLYEDHDRSELPEAA
                     QLASLGCGNPTAIADLQEGQTVLDLGSGGGIDVLLSARRVGPAGKAFGLDMTDEMLEL
                     ARSNQAEAGVENVEFLKGEIEAIPLPDDSVDVVISNCVINLSGDKPRVMREAARVLRP
                     GGRFAVSDVIADPDMSEETREDMKQWTGCIAGALTRDEFEANLLAAGFENVEIEETHR
                     VHDQAWSAIVRATMPS"
     gene            2854..3702
                     /locus_tag="PLV77_00340"
     CDS             2854..3702
                     /locus_tag="PLV77_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNC14329.1"
                     /translation="MTGDEIQLNDPQTLYERWEGAQWNPFTVPLGLDQEQWSGLGETD
                     RGLIYWVLSSLMVAEERITTKFSGLVGAHGSEEEASFLATQQVDEARHMQFYARFQNE
                     VIADPATVAAHVDRSREQVSPAFEQIFDVELVQAHEHLIANPGDLAAKVQFVTLYHLI
                     LESTLGLTTFKFVTDYLKEHELLPGFVDGYSKIHHDETRHIGYGVWFLRESVRDSPEV
                     APAAVREMLRKLLPSVAESLSPSSEPGGPDIEALGVDDEQIREFALGGLTRRLEIIGV
                     PLESVF"
     gene            complement(3691..4503)
                     /locus_tag="PLV77_00345"
     CDS             complement(3691..4503)
                     /locus_tag="PLV77_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNC14330.1"
                     /translation="MAGAQLKLVTWNVAGRTRLLTEQAAALSRTEPDLVTLQEIRQGT
                     VERWKAALAEDGLAHTIDSGYRTAERRLFNLTASRWKLSDLGDAPVPQPERVVSAALD
                     TPFGPCEIHNVHVPPAPSQGLAKLETIERLHDWLAVPGSHHRILCGDLNVPRLEYPDG
                     VIETFASNHPPEQFERWDRAEKLLLTGLAEYGLKDVFRSLHGYESPEPSWFPHRRNPG
                     PGLPGHRLDHVLASDSLNAVWAGYQHGWREAGLSDHSALEVIFEPRAGNLEN"
     gene            complement(4503..5267)
                     /locus_tag="PLV77_00350"
     CDS             complement(4503..5267)
                     /locus_tag="PLV77_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC14331.1"
                     /translation="MNPLNDLAKTVGFRRAGRTFDSPPPRFDPDGPPSTRELANEMLA
                     VAQLPRLFASSPRLLAAPRGDGSVVFDVPGWKAPEVSMQPIRTYLSCLGYDARTWGHG
                     TNRGSVEADVLQFRATVERAAEISERPVGLVGWSLGGVIAREVAREIPEAVSGVVTFG
                     SPIVGGPAHTVAARNYTEKESRRFEELIEKVNRENPLGVPITAIFTRRDGVVNWRACI
                     DRNTEGVRHVEVGSSHLGLGLDPDVWLATAEALAAA"
     gene            5357..5560
                     /locus_tag="PLV77_00355"
     CDS             5357..5560
                     /locus_tag="PLV77_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14332.1"
                     /translation="MRYELLEPSGICLEAFDCRDHAVEILIGMVADDSSNREITILEI
                     DDENEETLGFIEVDEELLASSTG"
     gene            5622..5876
                     /locus_tag="PLV77_00360"
     CDS             5622..5876
                     /locus_tag="PLV77_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14333.1"
                     /translation="MPTTKPRYTVTDTGDLEKLLDEAQGRWPDVKDRKDLLILLARTG
                     MAKVRADAENRRRVVDETSGALTGVYREDELSRLREDWPE"
     gene            5873..6259
                     /locus_tag="PLV77_00365"
     CDS             5873..6259
                     /locus_tag="PLV77_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNC14334.1"
                     /translation="MIVLDASLLIAHLDAGDAHHEEAEELLAGTGSARLGASTITLAE
                     ILVAPARQGKLEPAWAAVKKLGVEEIPVMADSAPRLAELRAKTGLKMPDCCVLEAARR
                     NNAKVATFDKSLANAAKGIGLTRSTG"
     gene            6311..>6552
                     /locus_tag="PLV77_00370"
     CDS             6311..>6552
                     /locus_tag="PLV77_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14335.1"
                     /translation="MAEPIPDLPSEKQRDQLVTLLRPLLHVTQPKLYGAENVAKAGSL
                     LVGNHTLYAFLDLPFMMAELWSKHQVTVRGLGDNAH"
BASE COUNT         1206 a         2030 c         2161 g         1155 t
ORIGIN      
        1 agcttggcgc cggtgccgcg cttgccggct gcggcgagat gaaccggatc gagaatgtcg
       61 gcgagcccga tcgtctccgc gtatgaggag gaaaaggtgg tcttgatctc cgaaacgacc
      121 cgatcgcgca ttgccatcac cgtctcgcca ccgagccgcc cgagcgcgtc ggtcagcaga
      181 tagccgccga ccgaccagcg catgccgaag gtgcgctgga tgatggtcgg accgaagtcg
      241 agcgagccgt aggtgtagac ctgcttgtgg acggccgatc cgtatggagt ccagaactca
      301 gccggcggct ggctgcgttc catcgccgaa aggatgtcgc tggccagggt tccgccaccg
      361 accgcgtcga aggccagggt agccccggtc gccgaaatgg cttcgaccag ccgttggtca
      421 aagtcgggca gggaggagtc gaccacatgt tcggcgccca gcgcctcgag tgtggcggcc
      481 tgctcgggct tgcgcacgat gttgaccagt ccgacctcgt cgtccttgca gatcttgacc
      541 agcatctggc cgaggttcga ggcagcggca gtgtgtacga gcgcggtgtg gccctcggcc
      601 cgcatcgtct cgaccatgga aagcgcggtc atcgggttca cgaagagggc ggccccgtcg
      661 gcgggggtgg cgtcgtcggg cagggggatg accgcggccc catcgaccag ccggtaatcg
      721 gaccacatcg ctcccccgac gagggagacc gtccgtccca cgagatcggc ggcctcgggc
      781 ccggcggcga cgaccgtgcc ggcgccttcg ttgccgaccg gcaacggttt gtcgagccgg
      841 tcgcgcatga tcggcagggc ctgttccggc accttgccgt tcaggccgcc gccttcggcc
      901 gggccgagcg tcgaggcatc ggccggaccg atcagaacac ccaggtcgga agggttcacc
      961 ggcgtcgcct cgactgagac cagcacctgg gacccggtcg gctcttcgat ctcctcctcg
     1021 atcaaccgga gctcgagggc tccgtcagag gtgactgtcg accgcaaact ccgtccgttg
     1081 atgggcatgg acgccattgt gaccaatttg acctcccccg tccacgaatg gccgacggga
     1141 gcccaaggcg tttacaggcc gaagtagggg cggagttctt cggggaccgc ttccgggtcg
     1201 ggctggaaaa tctccaccag caggtcgtcc ggaccccggg ccattgcgta tgaggcgtcg
     1261 ggctcggtcc ggacatcggt taccgagatg tccatgctgc ggagtctggc cacaacggtt
     1321 tcgatttcgt cggtctggac gccgaggtga tggaccaccg cctggccgga aaccttcggc
     1381 gcctggtcat acaggtggat ccggcccttg ccgacggtca tgaagacgtt cctggcgccg
     1441 gcgaactcgc ggtcgaagac gacctcgccc ccgaatcctt cggcccagaa gcggatcgtc
     1501 tcgtcgatgt cggaggccat caggtgggtg tggtggaggg agagcatcag gtgagttaag
     1561 actagacgcg tcttctttcc atcgaagttc atcggctcta ttgacaaata tcaatggaac
     1621 tgatctaatg cgctccgatg agccccgaac taaccctgac cccgaagcag aagagcccga
     1681 acggccagcc atgttgcgag ccggtcgtct atccggatgt tgaccgggcc cgggcggaac
     1741 ggatggcgaa ggtcgcgaag gcgctggggg acccgatccg gttgaccctg gtcgacgtcc
     1801 ttcgcaagca cgccggcaag gtctgcgtct gtgaactcgt gccgttgttt gatgtcaacc
     1861 agtcgacgct ttcccatcac ttgaagaagc tcagggacgc cggcatcgtt gattcggaac
     1921 gtcagggcct gtggtcctac tactacgtca accccgagga actagaggaa ctgaacgcat
     1981 ggctgagcta gagaagacca ccgactgctg ttcgcctgaa gaccaggcca cctgctgcga
     2041 acccgaagcc aagagcgaat gttgcggcga gggaccggaa tgcgggtgcc cggacggcgc
     2101 cgaaagaaca gattcggaac tccgggagat tgtccgtgag cgatatgccg ccgcggccag
     2161 ggacgcaggt tccagggacg catcctgctg cggcaacgat gccgtgatta ccgaggacca
     2221 gcgcgcggtg ttcggttcga gcctttacga ggatcatgac cgcagcgagc tgcccgaggc
     2281 cgcacagctg gcgtcactcg gctgcggaaa cccgactgcc atcgccgatc tccaggaggg
     2341 ccagacggtc ctggatctcg gatcaggcgg cggcatagac gtccttcttt cggccaggag
     2401 ggtgggcccg gccggcaagg ctttcgggct cgacatgacc gacgagatgc tggagctggc
     2461 aagaagcaac caggccgaag cgggcgtcga gaacgtcgag ttcctgaagg gggagatcga
     2521 agcgattccc cttcctgacg attcggttga cgtggtgatc tccaattgcg taatcaacct
     2581 ctccggtgac aagccccggg ttatgcgcga agcggcaagg gtccttcgtc cgggaggccg
     2641 gttcgcggtc tcggacgtaa tcgccgaccc ggatatgtcc gaggaaaccc gtgaggacat
     2701 gaagcaatgg accggctgca tcgccggtgc tctgacccgg gacgagttcg aggccaacct
     2761 cctcgcggcg ggcttcgaga acgtcgagat cgaagagacg caccgggtcc acgaccaggc
     2821 atggtcggcg atcgtgcgtg cgacgatgcc atcgtgaccg gcgacgagat tcaactcaac
     2881 gacccccaga ccctctacga acgctgggag ggtgcgcaat ggaatccctt caccgtcccg
     2941 ctcgggcttg accaggaaca gtggtccgga ctgggtgaga ccgatcgggg actgatctac
     3001 tgggtgctgt cttccctgat ggtcgccgag gaacgaatca ccacgaagtt ctccggcctg
     3061 gtcggcgccc acggctccga ggaggaggcg agcttcctcg ccacccagca ggttgacgag
     3121 gcccgacaca tgcagttcta tgcccggttc cagaacgagg tcatcgcgga tcccgcgaca
     3181 gtggcagcac atgtggatcg ttcccgggag caggtctcac ccgccttcga acagatcttc
     3241 gacgtcgaac tggtccaggc ccatgagcat ctgatcgcca accccggcga tctggccgcg
     3301 aaggtgcagt tcgtgacgct ctatcacctg attctggaga gcacgctcgg tctgaccact
     3361 ttcaagttcg tgaccgacta cctgaaggag cacgaactgc tccctggttt cgtcgacgga
     3421 tactcgaaga tccaccacga cgaaacccgg cacatcgggt atggggtctg gttccttcgc
     3481 gagtcggttc gcgactcgcc tgaggtcgcg ccagccgccg tccgggagat gctgaggaag
     3541 ctgcttccgt cggtcgccga gtcgctctcg ccgtcctcgg aacccggcgg cccggacatc
     3601 gaggcactgg gggtcgacga cgagcagatc agggagttcg cattgggcgg gctgaccagg
     3661 cgccttgaaa tcatcggcgt accgctcgaa tcagttttct aggttccccg cacggggctc
     3721 gaagatcact tccagggccg aatggtcgct aaggccagcc tcccgccaac catgctggta
     3781 cccggcccag acggcgttca gagagtctga cgccaggacg tgatcgagcc ggtggccggg
     3841 cagtcccggt cccgggtttc gccggtgggg gaaccagctg ggctcgggtg attcgtagcc
     3901 gtgcaggctt cgaaacacat ccttcagtcc gtattcggcg agcccggtca acagaagctt
     3961 ctcggcccgg tcccagcgct cgaattgttc gggagggtgg ttactggcga aggtctcgat
     4021 cactccgtcc ggatattcca gccggggaac gttcaggtct ccgcagagga ttctgtgatg
     4081 ggaacccggc accgccagcc agtcgtgaag ccgctcgatc gtctctagct tcgcgagacc
     4141 ctggctggga gcgggaggga cgtgaacgtt gtgaatctcg cagggcccga agggtgtgtc
     4201 cagggcggcc gagacgacgc gctcgggctg ggggactggc gcatctccga ggtcgctcaa
     4261 cttccaccgc gaggcggtca ggttgaatag tcgccgttcc gcggtccggt aaccggagtc
     4321 gatggtgtgg gcaagtccgt cttcggccag ggccgccttc catcgctcga cggtgccttg
     4381 ccgtatctcc tgcagcgtga ccaggtcagg ttcggtccgg ctgagtgcgg cggcctgttc
     4441 ggttagaagg cgagtgcgac cggccacgtt ccaggtgacc agtttgagct gtgcaccggc
     4501 catcaggccg cagcgagggc ctcggcggtg gccagccaga cgtcgggatc cagtcccaga
     4561 ccgagatggc tcgagccgac ttcgacgtga cggacaccct cggtgtttcg gtcgatgcaa
     4621 gcgcgccagt tcacgacccc gtcgcgccgg gtgaagattg cggtaatcgg cactcccagc
     4681 ggattctcgc gatttacctt ctcgatcagt tcctcgaagc ggcggctttc cttctcggtg
     4741 tagttgcgtg cggcgacggt atgggctggt ccgcccacga tcgggctgcc gaaggtgacg
     4801 acgcccgaaa ccgcctccgg aatctctctc gcgacttccc gggcgattac tccaccgagg
     4861 ctccagccga ccaggccaac cggccgctcc gagatctctg ccgccctttc caccgtcgcc
     4921 cggaactgga gcacgtcagc ctcgacgctg ccgcggttgg ttccgtgtcc ccaggttctg
     4981 gcgtcgtagc cgaggcagga gagatacgtc cggatcggct gcatcgagac ctcaggggcc
     5041 ttccagccag gtacgtcgaa gacaaccgat ccgtcccctc tgggggcggc gaggagtcgc
     5101 ggtgaggacg cgaacaggcg cggcagctgg gcaaccgcga gcatctcgtt tgcgagttcc
     5161 cgggtcgaag ggggtccgtc cgggtcgaat cggggcggag gggagtcgaa ggtcctgccg
     5221 gcgcgccgga agccaacggt ctttgccagg tcgttgaggg gattcacggg gggatggttt
     5281 ccagtttggg gccgtttgaa acccgacttc cttccagcgc ccgtttccgt ccgacccgtt
     5341 acctaggttc gcttacatgc gttacgagct cctggagcca tccggaatct gccttgaagc
     5401 attcgattgt cgcgatcacg cggtcgagat cctcatcggc atggtcgccg acgattccag
     5461 caaccgcgaa atcacgattc tcgagatcga cgacgaaaac gaagaaacgc tcggcttcat
     5521 cgaagtcgac gaagaactcc tcgcgtcgtc aacgggctaa ggcttcaaga gccaggcttt
     5581 gagggtgcga cacccaaata gtttggtgct acactctggc gatgccaacc acgaagcccc
     5641 gatacacggt gactgacacg ggggacctcg agaaactgct cgacgaggca cagggtcgtt
     5701 ggccggacgt gaaggaccgg aaggatcttc tgatcctgct ggcccggacc ggcatggcga
     5761 aggtcagggc tgacgctgaa aaccgtcggc gggtggtgga tgaaaccagc ggcgccctga
     5821 ccggtgtgta ccgcgaggac gagctctcca gactccgtga agactggccg gaatgatcgt
     5881 ccttgacgcc agcctcctga tcgcgcatct ggacgccggc gacgcgcatc acgaggaagc
     5941 ggaagagctt ctggcgggca ccggaagtgc caggctcggc gccagcacaa tcaccctggc
     6001 cgagatcctg gttgcgcccg ccaggcaggg gaaactcgaa ccggcctggg ccgcggtcaa
     6061 gaagctcggg gtcgaggaaa tccctgtgat ggccgactcg gctccgaggc tggctgagct
     6121 ccgagcgaag accggcctca agatgcccga ctgctgtgtg cttgaagcgg cacgacggaa
     6181 caacgccaaa gtggccacct tcgacaagtc actcgcaaat gcggcgaaag ggatcggcct
     6241 gacgcgatca actggctgaa gctcctccgg gcggcctgcg agagatcccg tgaagtagga
     6301 gaatcgtcgt atggcggagc cgatccccga cttgccatcg gaaaagcagc gggaccagct
     6361 ggtcacattg cttcggcctc tgttgcatgt cacccagccg aagctttatg gggccgagaa
     6421 tgtggcgaaa gccgggtcgc tcctggttgg caaccacacc ctttacgcat tcctcgacct
     6481 tccgttcatg atggccgaac tctggtcgaa gcatcaggtg accgtccgcg ggctcggtga
     6541 caacgcccac ta
//
LOCUS       DAOQMF010000010         6270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_5323_length_6270_cov_3.680290, whole genome shotgun sequence.
ACCESSION   DAOQMF010000010 DAOQMF010000000
VERSION     DAOQMF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5323_length_6270_cov_3.680290"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLV77_00375"
     CDS             <1..724
                     /locus_tag="PLV77_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNC14336.1"
                     /translation="RVPVRARESLETLADHLALVACITGRPSPVARKMVGAKKITYFG
                     NHGLSFIKPGQDRPTVVIEGEEAERARNFIRENDNPFWVTSKIRIEDKGPIQALHWRG
                     AGHRTEEVIRGIADHAQSAGLATHWGRKVLELRPPGMEGKAGAVDELLDGTDIDTVVF
                     AGDDRTDYDAAVRLKKLADEGQLKRLLLVAIDSDEGPPELVELADLVVAEPEEWIDLI
                     EDLAGTFSREPAEAQPGAPQPE"
     gene            728..1240
                     /locus_tag="PLV77_00380"
     CDS             728..1240
                     /locus_tag="PLV77_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14337.1"
                     /translation="MSFAELLRATVFLAAGGATALGAIAVIGVRDAENTTILLIGAVW
                     WIVAAWIGLSLGASDRSADAMRETLANARTVNSNSPGVSMPSPGRVAWGRLSPILGAV
                     GLAGALGVVFPEVAVVGSGYALLVALAWRNREAAVLAIEGRDGVRFLVESASPLKPVK
                     LVRSPGFRAF"
     gene            1309..2229
                     /locus_tag="PLV77_00385"
     CDS             1309..2229
                     /locus_tag="PLV77_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HNC14338.1"
                     /translation="MADGWSSPNYDEWRETCDTLHAHTQFLGKLCVALAPPEPQLQHA
                     ALRLTARGWETLPLPSPNGNGAFVAALDLRTHHLLIEHSDGMGADIALAPNRPVGEVV
                     REGLTAIDELAGPVEIDPAPQEVDWTAPLDEDDEHRIYDPVQVKAYFEMATAAGMALT
                     RFRAPFRGRSTPLNAWWGSFDLAVNLFSGRPAEPPSQDFIMRNAMDAQEVAVGWWPGD
                     GRYGKPAFYAYAHPAEDGFAGRDLSPVPGGWNEELGEYVFDHEEAAATGNPEQAVIDF
                     CGEIFSHACSVCDWNPALAASAAGDPPPVR"
     gene            complement(2300..4003)
                     /gene="polX"
                     /locus_tag="PLV77_00390"
     CDS             complement(2300..4003)
                     /gene="polX"
                     /locus_tag="PLV77_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNC14339.1"
                     /translation="MTNARIAEALEELAVLYQLDGADQYRVLAYTNSARTIKNEGRTV
                     EEMARNGTITELPGIGKTLEEKIVALVETGEIPAAAKLKERIPVGLLEISRIPGLGPK
                     TVRRLHDELDVNGPEDLRAAAEAQKVRELKGLGPKVEEKILAGLDRLDAEPEGPTRLR
                     LDEILPTAEELAAALRAESSCDRAEIAGSVRRLAETCHDIDLIAASGSRKELAAAIAE
                     HELVAEHGNPNETGIRLTTNSGIGVDVRIVEPGAFGHLLQHFTGSGPHNAELRERAVA
                     QGLHVSENGIKDDETGETEFFATEEEIYERLGYQYIPPELREDRGELDAAARGELPEL
                     IERGQIKGDLHSHTTLSDGVASLDEMAAAAEALGYEYLAITDHSESHGFGNHVEADRL
                     WQRIEEIAELNNEDRKIRLLSGSEVNIHPNGSVDYEDDLLETLDWVIASIHTAFSDDA
                     KKNTDRMITAIENPLVDCIGHPTGRLINQRDPYPLDMERVIAAAVENDTLIEINSNPR
                     RRDLNEIHARMAAEAGVGIVINTDAHRPESLDFIEYGIATARRAWLTADQVRNTGPWW
                     K"
     gene            4047..4784
                     /locus_tag="PLV77_00395"
     CDS             4047..4784
                     /locus_tag="PLV77_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNC14340.1"
                     /translation="MPDEARTEHRGAAWVILPTYNEAGNVEKMVDALGPKLTHDDDRI
                     LIVDDNSPDGTGQIADRIATENPRVRVLHRKVKEGLGPAYIEGYRVALEGGADLIVQM
                     DADFSHDPAYLPRLLAASELADLVIGSRYVPGGGITEWGQVRRLLSRGGSLYSRTVLR
                     VPVRDMTGGFKCFRRKVLETIDLSQVAASGYSFQVEMTWRVVQAGFSVMEVPITFRER
                     QEGDSKMTTAIVAEAAWRVPAMRIRRH"
     gene            4924..5250
                     /gene="trxA"
                     /locus_tag="PLV77_00400"
                     /pseudo
     CDS             4924..5250
                     /gene="trxA"
                     /locus_tag="PLV77_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            5271..5447
                     /locus_tag="PLV77_00405"
     CDS             5271..5447
                     /locus_tag="PLV77_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNC14341.1"
                     /translation="MPFVQIHWFEGRSDEQKAEIAKRIEEALIDVAGAAPEHCWVKFE
                     DSKKTDFIIPEARS"
     gene            5610..5981
                     /locus_tag="PLV77_00410"
     CDS             5610..5981
                     /locus_tag="PLV77_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC14342.1"
                     /translation="MGVLNTDLGRQLEAKELGARLWRIEPGQASTRHRHKTTEELYLL
                     LEGTGKLRIGEELLTLEPMDAVVVEPETVRQPFNDTDADQLWLIVGAPTEHADTTSMD
                     EELIAWMYPDGPKAMPPELDP"
     gene            6009..>6270
                     /locus_tag="PLV77_00415"
     CDS             6009..>6270
                     /locus_tag="PLV77_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC14343.1"
                     /translation="MERPESAAQVVGKRTIFEAEHDDYRESFRRFLEKEVVPEYPQWE
                     RDHIVPKSLFTKCAEYGFLSMEVPEEYGGPGVDDWRFNVVLAE"
BASE COUNT         1017 a         2030 c         2123 g         1100 t
ORIGIN      
        1 ccgtgttccg gtccgggccc gggaaagcct cgaaaccctg gccgaccacc tcgcgctggt
       61 cgcctgcatc acgggtcgcc cctcgccggt cgcccggaag atggtcggag ccaagaagat
      121 cacctacttc ggcaatcacg gcctcagctt catcaagccc ggccaggacc ggccgaccgt
      181 ggtgatcgag ggcgaagagg cggagcgggc ccggaacttc atccgcgaga acgacaaccc
      241 cttctgggtg acctcgaaga tccggatcga ggacaagggt ccgatccagg ccctccactg
      301 gcgcggcgcc ggccaccgga ccgaggaagt aatcaggggc atcgccgacc acgcccagtc
      361 ggccgggctt gcgacccatt ggggccggaa ggtactcgag cttcgtcccc ccgggatgga
      421 aggcaaggcc ggcgcggtcg acgagctgct cgacggaacc gacatcgaca cggtggtctt
      481 cgccggcgac gaccggaccg attacgacgc cgccgtccgc ctcaagaaac ttgccgatga
      541 gggtcagctg aagcgcctgc tgctggtcgc gatcgactcg gacgaaggcc cgccggaact
      601 ggtcgaactc gcggaccttg tcgtggccga gccggaggag tggatcgacc tgatcgaaga
      661 tctggcgggc accttcagcc gggagccggc ggaagcccag cccggcgcgc cgcaaccgga
      721 gtagggcttg tccttcgccg agctgctcag ggccacggtt ttcctggctg ccggtggtgc
      781 gacggcgcta ggggcgatcg cggtgatcgg ggttcgggat gccgagaaca cgacgatcct
      841 gctgatcggc gcggtctggt ggatcgtcgc cgcctggatc ggcctcagcc tgggcgcgtc
      901 cgaccgctcc gccgacgcga tgcgcgaaac gctggccaac gccaggaccg tcaactccaa
      961 ctcgccggga gtctcgatgc cctcgccggg gcgagtcgcc tgggggcgcc tcagcccgat
     1021 cctcggcgcg gtcggtctgg ccggtgcgct cggcgtcgtc ttccccgagg tggcggtggt
     1081 cggttccggc tacgccctgc tggtcgcttt ggcctggcga aaccgcgagg ccgcagtcct
     1141 ggcgatcgag ggacgggacg gcgtgcgctt cctggtcgaa tcggcctcgc cgctcaagcc
     1201 ggtcaagctg gtccgtagcc ccggcttccg cgccttctga gaccttcggc gggacccgag
     1261 gtgatgctgg ttcgtccagc ccgatttcgg ggggagtaag gtcgaagcat ggctgacgga
     1321 tggagttccc ccaactacga cgagtggcgc gagacctgcg acaccctcca cgcccacacg
     1381 cagttcctcg gcaagctctg cgtcgccctg gctccccccg agccgcagct gcagcatgcg
     1441 gcgctaaggc ttaccgcaag gggttgggag actctgccgc tgccgtcccc gaacggcaac
     1501 ggagccttcg tggcggcgct cgaccttcgc acccaccacc tgctgatcga gcattccgac
     1561 ggcatgggtg cggacatcgc cctcgccccg aatcgcccgg tcggcgaggt cgtccgggaa
     1621 gggctgaccg cgatcgatga attggctggt ccggtggaga tcgatccggc tccccaggaa
     1681 gtggactgga cggcgccgct ggacgaggac gacgagcacc ggatctatga ccccgtgcag
     1741 gtcaaggcct atttcgagat ggccaccgcg gccggaatgg ccctgacccg tttccgcgct
     1801 cccttccgcg gccgctcgac tccgctcaat gcctggtggg gttccttcga cctcgccgtg
     1861 aatctcttct cgggccggcc cgcggaacca ccctcgcagg acttcatcat gcggaacgca
     1921 atggatgccc aggaagtcgc ggtgggctgg tggcccggtg acggccgcta cggcaagccg
     1981 gccttctacg cgtatgccca tccggccgag gacggcttcg ccggccggga cctttcaccg
     2041 gtaccgggcg ggtggaacga agagctgggc gagtacgtgt tcgaccatga ggaggccgcc
     2101 gcgaccggaa acccggagca ggcggtgatc gacttctgcg gcgagatctt cagccacgcc
     2161 tgcagcgtct gcgactggaa cccggccctg gccgccagcg ccgccggtga tcctcccccg
     2221 gttcgctagc tccgattcgc atcagaagcc acggttttcc acagtatttg tggaaaatcg
     2281 tgggtcttga ccgacctcgc tacttccacc aggggccggt gttccggacc tggtcggcgg
     2341 tcagccaggc ccggcgggcg gtcgcgatcc cgtactcgat gaaatcgagg gattcgggcc
     2401 ggtgggcgtc ggtgttgatc acgatcccga ctcccgcctc ggccgccatg cgggcgtgga
     2461 tctcgttcag gtcgcggcgg cggggattgg agttgatctc gatcagggtg tcgttctcga
     2521 ccgcggcggc gatcaccctc tccatgtcga ggggataggg gtctcgctgg ttgatcaggc
     2581 ggccggtcgg atggccgatg cagtcgacca gcgggttctc gatcgcggtg atcatgcggt
     2641 cggtgttctt cttcgcgtca tcgctgaagg cggtgtggat cgaggcgatc acccagtcca
     2701 gggtctcgag caggtcgtcc tcgtaatcga ccgaaccgtt cggatggatg ttcacctcgg
     2761 atccggatag cagccggatc ttgcggtctt cgttgttgag ctcggcgatc tcttcgatcc
     2821 gttgccagag ccggtcggct tcgacgtgat tgccgaagcc atgggattcg gagtggtcgg
     2881 tgatggccag gtactcgtag ccgagcgctt cggctgccgc ggccatctcg tcaagtgagg
     2941 cgacgccatc ggagagggtg gtgtgggagt ggaggtcacc cttgatctgg ccccgctcga
     3001 tcagctcggg caactcgccc ctggcggcgg cgtcgagttc cccccggtcc tcgcggagtt
     3061 cgggcgggat gtactggtag ccgaggcgct cgtagatctc ttcctcggtc gcgaagaact
     3121 ccgtttcacc cgtctcgtca tccttgatcc cgttctcgga cacatgcagc ccctgggcga
     3181 ctgcccgttc gcggagctcg gcgttatggg ggccggagcc ggtgaagtgc tggagcaggt
     3241 ggccgaaggc accgggctcg acgatccgca cgtccactcc gattccggag ttggtggtca
     3301 acctgatccc ggtctcgttc ggattgccgt gctcggcgac cagttcgtgt tcggcgatcg
     3361 cggcggccag ctccttgcgt gagccggaag cggcgatcag gtcgatgtca tggcaggtct
     3421 cggcaagccg gcggacggag cccgcgatct cggccctgtc acagctcgac tccgctctga
     3481 gcgcggcggc gagctcctcc gcggtcggga ggatctcatc gagacgcagc cgggtcgggc
     3541 cttccggttc ggcgtcgagc cggtcgaggc cggcgaggat cttctcttcg accttcggtc
     3601 ccagcccctt gagttcacgg accttctgcg cctcggcggc ggcgcgcagg tcttcgggcc
     3661 cgttgacatc gagttcgtca tgcaggcggc ggacggtctt cgggccgagc ccggggatcc
     3721 tgctgatctc gagcaggccc accgggatcc tctccttcag cttcgccgcg gccgggatct
     3781 cgccggtttc gaccagggcg acgatcttct cttcaagcgt cttgccgatc ccgggcagtt
     3841 cggtgatcgt gccgttccgg gccatctcct cgaccgtacg gccctcgttc ttgatcgtcc
     3901 gggccgagtt cgtgtaggcg aggacccggt actgatcggc cccgtccagc tggtagagaa
     3961 cggccagctc ttcaagcgct tcggcgatcc gggcgttggt catacccaga aactacctcc
     4021 aaccctttga ggggtacgat ccgccggtgc cggatgaagc caggacagag caccgcggcg
     4081 cggcctgggt catcctgccg acctacaacg aggccggcaa cgtcgagaag atggtcgatg
     4141 cactcggtcc gaagctgacc catgacgacg accggatcct gatcgtcgac gacaattccc
     4201 cggacggtac cgggcagatc gccgatcgga tcgcgaccga aaatccccgg gtccgggtgc
     4261 ttcaccgcaa ggtcaaggaa ggtctcggac ccgcttacat cgaggggtac cgggttgcgc
     4321 tcgagggcgg ggccgacctg atcgtccaga tggacgccga cttctcccac gacccggcct
     4381 acctgccgcg gcttctggcc gcctcggagc ttgccgacct ggtgatcggc tcgcgctacg
     4441 tgcccggcgg ggggatcacc gaatggggcc aggtccggcg actgctcagc cgtggcggca
     4501 gcctttactc gaggacggtt ctgcgggttc cggtccggga catgacgggt ggcttcaagt
     4561 gcttccggcg gaaagtcctc gaaacgatcg acctctccca ggttgccgcc tccggttatt
     4621 ccttccaggt cgaaatgacc tggcgggtcg tccaagccgg tttctcggtg atggaggtac
     4681 cgatcacctt ccgcgaaagg caggaaggcg attccaagat gacaactgcc atagtcgccg
     4741 aggcggcatg gcgagtcccg gccatgcgaa tccggaggca ttgacccccg gagccggccc
     4801 atctaacctt gagccctgtg ggccggcggg ttcgaccggg aggggggtcg gaccccgccc
     4861 gactagtttt cccccatact tcattttgta tagtactagt accagttcaa cgatcaggag
     4921 accgacatgg cacttcccga agtaaccgac gcgacgttca aggccgaagc acttgaagcc
     4981 gatggcccgg tgctggtcga cttctgggcc ccttggtgcg gtccctgccg tgtggtccac
     5041 ccggtgctcg aagagatcaa cgaagagcgc gacgacctca agatcatcag cctcaacatc
     5101 gatgacaacc cgcagaccgc ccagcagtac ggggttctct cgatcccgac gatgatcctg
     5161 ctcaaggacg gcgccctggt caagcagctg gtcggcgccc agcccaagcg caagattctc
     5221 gccgagctcg agccggccct tcagggctag ccggacgggg acggatcacc gtgccgttcg
     5281 tgcagatcca ctggttcgag ggccgctccg atgagcagaa ggccgagatc gccaagcgca
     5341 tcgaagaggc gctgatcgac gtcgccgggg cagcccccga acattgctgg gtcaagttcg
     5401 aggactcgaa gaagaccgac ttcatcattc ccgaggcgcg ttcgtagcgg ggtcgagggg
     5461 gtgagcgccg tgggggtacc cttggtgtgc acagtcccag tcgggcaccc ccacggcgcc
     5521 cacccctcag aacctggagg aggaccggtc ccgtgagtgg gtttcgtcgg ttgagggtgg
     5581 gggagcagtt ctggcggcgt tctaaccaga tgggagttct gaacaccgat ctgggtcggc
     5641 agcttgaggc gaaggaactc ggggccaggc tctggcggat cgagccgggc caggcctcga
     5701 cccggcaccg gcacaagacg accgaggagc tttacctttt gctcgaaggc accggcaagc
     5761 tgcggatcgg ggaggagctc ctgacactcg aaccgatgga cgcggttgta gtcgagccgg
     5821 agacggtgcg ccagccgttc aacgacaccg atgccgacca gctctggctg atcgtcggcg
     5881 ctccgaccga gcatgccgac accacatcga tggacgagga gctgatcgcc tggatgtacc
     5941 cggacgggcc gaaggcgatg ccgcccgagc ttgacccctg atctgcgcaa gcggactagg
     6001 cttccggcat ggagagaccg gagagtgcag cgcaggtagt cggcaagcgg accattttcg
     6061 aagcggagca cgacgactat cgcgagagct ttcgtcgttt cctcgagaag gaagtggtcc
     6121 ccgagtaccc gcagtgggag cgggatcaca tcgttcccaa gagcctgttc accaaatgcg
     6181 ccgagtacgg cttcctctcg atggaagtgc ccgaggagta cggcggcccg ggcgtcgatg
     6241 actggcgctt caacgtggtg ctggccgagg
//
LOCUS       DAOQMF010000011         6148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_5494_length_6148_cov_3.174955, whole genome shotgun sequence.
ACCESSION   DAOQMF010000011 DAOQMF010000000
VERSION     DAOQMF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5494_length_6148_cov_3.174955"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="PLV77_00420"
     CDS             <1..1536
                     /locus_tag="PLV77_00420"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC14344.1"
                     /translation="AEAMAEAAASDARRANGQAMGPLDGIPYTAKDSYLVRGMTAASG
                     SPAFVNLLAMRDAFTVKRLREGGAICLGKTNMPPMANGGMQRGVYGRAESPYNADYLT
                     APFASGSSNGAGTATAASFAAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWP
                     LTPTMDVVVPYARTMADLLEVLDVIVADDPDTRGDLWRAQEWIELPAASEVRPDSYPS
                     LAASSEALAGNRFGIPRMYINADPAAGTGEKSDVWGPTGERIDTRQSVIDLWQAARND
                     LEAAGAEVVEVDFPVVSNYEGDRAGCPNIFTRGLVSPAFLDEELRGLSAWAYDDFLRA
                     NDDPALNRLADADGELIFPPEAGALPSREGDLAFGMEEYVEMAWAGLTPWDQLPELEA
                     GLRGLEQTRRIDLEEWMDELGLDTVVFPAVADVGPADADVNPRSADRAWANGVWVANG
                     NLVPRHLGIPTVTVPMGLMGDIGMPVGLTFAGRSYDDNRLLTQASAFESIRPRRIPPP
                     RTT"
     gene            complement(1552..2769)
                     /locus_tag="PLV77_00425"
     CDS             complement(1552..2769)
                     /locus_tag="PLV77_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC14345.1"
                     /translation="MIYLETPKKFRPLVTQANHVAREIFRPISRKYDEAEHEYPKELD
                     VLASLINGMNEGSGMGAGAAGVSGESGDEPDSGSGNRNGSNLSTVLGIIELCWGDVGL
                     LLAMPGQGLGNSAIASVATAEQLERYGGKWAAMAITEPEAGSDSAAIRTTAVLDGDEY
                     VLNGEKIFVTSGDRAELIVVWASLDRSVGRAAIKSFIVERDNPGLTLDRLEHKLGIRA
                     SDTANFTLQDCRVPKEALLGSPEVDVKKGFAGVMKTFDNTRPLVAGMALGVTRAALQE
                     TKRQLEAAGVEIDYDTPALCQSAAAARYLEMEADYEAALMLTLQAAWMADNRKPNSLE
                     ASMAKAKAGRTGVDVTLGCVEICGSIGLGESELLEKWSRDAKILDIFEGTQQIQLLII
                     ARQLLEKTSAELK"
     gene            complement(2795..4138)
                     /locus_tag="PLV77_00430"
     CDS             complement(2795..4138)
                     /locus_tag="PLV77_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC14346.1"
                     /translation="MSSKNSQGLADKTMGSSLRALNWIAGSKILDRLRLRSQFERTVF
                     QSTKHGMRAATSAGRSFNKATSLGKPARQKSGSGSGLFDLTPDDEQQMFIEAGQAFAA
                     DQIRPAASGADSEKATPAAILEQAGEIGVNTLGIPEEFDGVLTEQSTVTWTLMAEALA
                     HGDMGIAYAALAPGAAATAIGRWGDPDQQASYLPAFTGENPPAAALAILEPKPLFDPF
                     NPDTRATAGTGGHWILNGVKSMVARPGECELFIVAAATGDARPTLFIIESGSEGLTFE
                     AEPAMGLRAASTGRLVLEDVRVPASAMLGEEGDRARIYADCVSGSRIAWSAMFSGTAQ
                     AVLDYVIPYVNERQAFGEPISNRQSVAFSVSDIAIETGGMRLANWRAASRADRGESFA
                     REAALARRLAAAKGMDIGSAGVQLLGGHGYVKEHPVERWYRDLRTAGVMEGALLA"
     gene            complement(4140..5048)
                     /locus_tag="PLV77_00435"
     CDS             complement(4140..5048)
                     /locus_tag="PLV77_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNC14347.1"
                     /translation="MTRLESSPSILPLYLKAAMPLIPGASSLPFLPGGGGEIPSGLGF
                     ELKGVVPQQDQVAEYAKVCGFPLKDRLPPTFPHVLAFPLHMAVMSNGEFPFGAVGLVH
                     IENSINQLRPIAFGEMLEVKVEVTSLSPHPKGQIFSLLTEVSAAGETVWTETSTMLRR
                     GKGSKKPSAPGLEIPTLDPGTPASATWKLPADLGRRYGAVSGDRNPIHMHALTAKPLG
                     FHSAIAHGMWTAARALAQIESRLPDSFTAGVRFRKPIFLPARVQFAAEGDGTETLFAV
                     RDARKGTPHMDGRITSNANETAKASN"
     gene            complement(5048..5638)
                     /locus_tag="PLV77_00440"
     CDS             complement(5048..5638)
                     /locus_tag="PLV77_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC14348.1"
                     /translation="MTSRLGGRSLTLDITTPDAPDAIARELGEGVDLIVHNAGVTLDR
                     TIAKMPEERWSKLMEINLSSEERINDAVLSADLLNANGRIVCVSSMSGIAGNSGQTNY
                     AASKAGVIGMVESMAPELARRQATINAVAPGFIETQMTAAMPFGTREVGRRLNSLSQG
                     GKPVDVAETIAWFASPASTGVNGNVVRVCGQNLLGA"
     gene            complement(5635..>6148)
                     /locus_tag="PLV77_00445"
     CDS             complement(5635..>6148)
                     /locus_tag="PLV77_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14349.1"
                     /translation="KALIFDATGISDSTELVELQRFFYPAVPRLAPSGRAIVLGSPPA
                     ATGGVRAHTAQRALEGFVRSLGKEIGGRGSTAQLVQVEPGAGDEIESTLRFLLSPRSA
                     FVSGQVIRVGKPVAPKPELDWERPLAGRTALVTGASRGIGEAIATVLARDGRPSSDST
                     FRPPRPSWTR"
BASE COUNT          948 a         2009 c         2132 g         1059 t
ORIGIN      
        1 gcggaggcca tggcggaagc agccgcgtcc gatgccaggc gggccaacgg ccaggccatg
       61 ggcccgcttg acggaatccc ttacaccgcc aaggacagct acctggtgcg cggcatgacg
      121 gctgcctccg ggagcccggc cttcgtcaac ctgctggcga tgcgtgacgc cttcacggtc
      181 aagcggctgc gggaaggggg cgcgatctgc ctcggcaaga ccaacatgcc gccgatggcc
      241 aacggcggca tgcagcgggg cgtctacggc cgcgcggaga gcccctacaa cgccgattac
      301 ctgaccgcgc cgttcgcctc gggatcatcg aacggggccg ggaccgcgac cgcagccagt
      361 ttcgccgcct tcggtctggc cgaggaaacg tggtcgagcg gcagggggcc ggcctcgaat
      421 aacggcctct gcgcctacac gccttcacgc ggagtgattt cggtccgggg caactggccg
      481 cttaccccga ccatggacgt ggtggttccc tacgcgcgaa ccatggccga tctgctcgag
      541 gtgctcgacg tgatcgtcgc cgatgatccc gacaccaggg gagacctctg gcgggcgcag
      601 gagtggatcg agctgccggc cgcctccgag gtcagaccgg actcgtaccc gtcgctggcc
      661 gcatcttccg aggcgctggc cggcaatcgg ttcggtatcc cgcgcatgta catcaacgcc
      721 gatccggccg cgggcacggg ggagaagagc gacgtctggg gaccgaccgg cgagcgcatc
      781 gatacccggc agtcggtgat cgacctctgg caggcagccc gcaatgatct cgaggcggcc
      841 ggggccgagg tggtcgaagt cgatttcccg gtggtctcca actacgaggg tgaccgggcc
      901 ggctgtccga acatcttcac ccgcggcttg gtcagcccgg cctttctcga cgaggagttg
      961 cgcggccttt cggcctgggc gtacgacgac tttctccggg ccaatgatga cccggccctg
     1021 aaccggctgg cggacgcgga cggcgagctg atctttccgc ccgaagccgg tgccctgccc
     1081 agccgggaag gtgacctggc cttcggcatg gaggagtatg tggagatggc ctgggccggc
     1141 ctcaccccct gggatcaact gcctgaactc gaagcaggcc tgcggggtct ggagcagacc
     1201 cgccggatcg acctcgagga gtggatggat gagctcggac tggacacggt ggtctttccg
     1261 gcggtcgccg acgtcggtcc ggccgacgcc gatgtgaacc cgcgctcggc cgaccgggcc
     1321 tgggccaacg gtgtctgggt ggcgaacggc aacctcgttc cccggcacct cggcatcccg
     1381 accgtgaccg tgccgatggg gctgatgggt gacatcggga tgccggtcgg cctcaccttc
     1441 gccggtcgtt catacgacga caaccggctg ctcacccagg cctcggcttt cgaatcgatc
     1501 cgcccccgac gcatcccgcc accgcggacc acttgagccg ggggcttctg gctacttgag
     1561 ttcggccgag gtcttttcga gcagctggcg ggcgatgatc agcagctgga tctgctgggt
     1621 gccttcgaaa atgtcgagga tcttcgcgtc acgggaccac ttctcgagca gctcggattc
     1681 accgagaccg atgctgccgc agatctcgac gcagcccagg gtcacgtcga ccccggtccg
     1741 gccggccttg gctttggcca tcgacgcttc gagcgagttc ggcttccggt tgtcggccat
     1801 ccaggcggcc tgcagggtca gcatcagggc cgcctcgtaa tcggcttcca tctcgaggta
     1861 gcgggccgcg gccgcggact ggcaaagcgc cggagtgtcg taatcgatct cgacaccggc
     1921 cgcttcgagc tggcgcttcg tctcttggag cgccgcgcgg gttaccccga gggccatgcc
     1981 ggcgaccagg ggcctggtgt tgtcgaaggt cttcatcact ccggcgaagc ccttcttcac
     2041 gtccacctcc ggggagccga gtagcgcctc cttcggcacc cggcagtcct gcagggtgaa
     2101 gttggcggtg tccgacgccc ggatgcccag cttgtgctcg agccggtcca gggtcaggcc
     2161 cggattgtca cgctcgacga tgaacgactt gatcgccgcc cggccgaccg agcggtcgag
     2221 gctggcccag accacgatca gctcggcccg gtcaccggaa gtgacgaaga tcttctcgcc
     2281 gttcagcacg tattcgtccc cgtcgaggac ggctgtagtg cggatcgccg cggaatccga
     2341 gcccgcctcc ggttcggtga tcgccattgc ggcccacttg ccgccgtagc gctcgagctg
     2401 ctccgcggtc gcgaccgacg cgatcgccga gttgccgagc ccctggccgg gcatcgccag
     2461 aagcagtccg acgtcacccc agcagagctc gatgatgccg agcacggtgc tcaggttcga
     2521 accgttgcgg ttgccgctgc ccgaatccgg ttcatcaccg gattccccgc tgacgccggc
     2581 cgcaccggcg cccatgcccg aaccctcatt catgccgttg atcagcgagg ccagtacgtc
     2641 gagttccttc gggtactcgt gttcggcctc gtcgtacttg cgcgagatcg gacggaagat
     2701 ctcgcgggcg acgtggttgg cctgggtgac cagcgggcgg aacttcttcg gtgtttccag
     2761 atagatcatg gtttcatttc gctcggcctg gtgatcaggc gagcagggcg ccctccatca
     2821 cgcccgctgt gcggaggtcg cgataccagc gttcgaccgg gtgctccttg acgtagccgt
     2881 ggccgcccag cagctggact ccggccgagc cgatatccat gccctttgcg gcggcgagcc
     2941 ggcgggccag ggctgcttcg cgggcgaacg attccccgcg gtcggcccgg ctcgcggccc
     3001 gccagttggc gaggcgcatg ccaccggttt cgatcgcgat gtcggaaacg ctgaaggcca
     3061 cgctctggcg gttcgagatc ggctcgccga aggcctgacg ctcgttcacg taggggatca
     3121 cgtagtcgag cacggcctgg gcggtgccgg aaaacatcgc cgaccaggcg atccgggacc
     3181 ccgaaacgca gtcggcgtag atccgggcgc ggtcgccttc ctcgccaagc attgccgagg
     3241 caggaacgcg cacatcttcc agcaccagcc ggccggtcga tgcggccctg agccccatcg
     3301 cgggttcggc ttcgaaggtc aggccctcgg agccggattc gatgatgaag agcgtcggcc
     3361 tggcgtcacc ggtggcggcc gcgacgatga agagctcgca ctcgccgggc cgggcgacca
     3421 tcgacttcac cccgttgagg atccagtggc cgccggttcc ggcggtggcc cgggtgtcgg
     3481 gattgaaggg atcaaagagc ggcttcggct cgaggatcgc cagcgcggcg gccggcggat
     3541 tttcgccggt gaaggcgggc aggtagctcg cctgctggtc ggggtcgccc cagcggccga
     3601 tcgcggttgc tgccgcgccg ggggccagcg ccgcataggc gatgcccatg tcaccgtggg
     3661 cgagggcctc ggccatcagg gtccaggtta cggtcgactg ctcggtcagg accccgtcaa
     3721 attcctcggg gatgccaagt gtgttgacgc cgatctcgcc ggcctgctca aggatcgcgg
     3781 ccggagtggc cttctccgag tccgccccgg aggcggcggg ccggatctgg tcggccgcaa
     3841 aggcctgccc ggcctcaatg aacatctgct gttcgtcatc cggagtgaga tcgaagagcc
     3901 cggatccgga gccgctcttc tggcgggctg gcttgccgag gctggtcgcc ttgttgaagc
     3961 tgcggccggc gctggtcgcg gcgcgcattc cgtgtttggt gctctgaaag acggtccgct
     4021 cgaactggct gcgcagcctg agccggtcga ggatcttcga tccggcgatc cagttcaggg
     4081 cacgaaggct ggagcccatc gtcttgtcgg ccagcccctg gctgttcttg ctgctcattt
     4141 caattgctcg ctttcgcggt ttcgttcgcg ttggaagtga tgcgaccgtc catgtgaggg
     4201 gtgcccttgc gggcatcgcg gacggcaaag agggtttccg tgccgtcgcc ttcggcggcg
     4261 aactgaaccc gggcgggcag gaagatcggc ttgcggaacc tgaccccggc agtgaaggaa
     4321 tcgggcagcc ggctctcgat ctgggccagg gcccgggcgg cggtccacat cccgtgagcg
     4381 atcgcggagt ggaagcccag tggctttgcg gtcagggcat gcatgtggat cgggttgcga
     4441 tccccggaga ccgcgccgta gcgccggccg aggtcggccg ggagtttcca ggtggcgctg
     4501 gccggagtcc cggggtcaag cgtgggaatt tcgaggcccg gagccgaggg cttcttactg
     4561 cccttcccgc gccgcagcat ggtgctcgtc tcggtccaga ccgtctcccc ggcggcactg
     4621 acctcggtca acagggaaaa gatctgcccc ttcgggtggg gggagaggga tgtgacttcg
     4681 actttcactt caagcatctc gccgaatgcg atcggcctga gttgattgat cgagttttcg
     4741 atgtgaacca ggccgaccgc gccgaaggga aactccccgt tgctcatcac cgccatgtga
     4801 agcggaaagg ccagcacgtg ggggaaggtt ggcggcagcc tgtccttcaa cgggaacccg
     4861 cagaccttgg cgtattcggc tacctggtcc tgttgcggaa ccactccctt cagttcgaag
     4921 ccgagcccgg aaggaatctc cccgccgccg ccaggcagga agggcaggga ggaggcgccg
     4981 gggatcagcg gcatcgccgc cttcagatag agcggcagaa tcgacgggga ggattcgagc
     5041 ctggtcatca ggctcccagc aggttctggc cgcagaccct aaccacgttt ccgttgaccc
     5101 cggttgaggc ggggctggcg aaccaggcga tcgtctccgc cacgtcaacc ggcttgccac
     5161 cttgcgagag gctgttcagg cggcggccga cctcacgggt gccgaatggc atcgccgcgg
     5221 tcatctgcgt ctcgatgaat ccgggggcga ccgcgttgat cgtggcctgt cgccgcgcca
     5281 gctcgggcgc catcgattcg accatgccga tcacaccggc cttggaagcg gcgtaattgg
     5341 tctggcctga attgccggcg atgccggaca tcgatgaaac gcagacgatc cggccgttgg
     5401 cgttcagcag gtcggcggaa aggaccgcgt cattgatccg ttcctcgctg gagaggttga
     5461 tttccatcag cttgctccag cgctcttccg gcatcttggc gatcgtgcgg tccagagtga
     5521 cgccggcgtt gtggacgatc aggtcgactc cttcgccgag ctcgcgggca atcgcgtcag
     5581 gcgcatcggg ggtcgtgatg tccagggtca gggaacgccc gccgaggcgc gaagtcacct
     5641 ggtccagctc ggccgcggcg ggcggaatgt cgagtccgat gacggtcgcc cgtcccgcgc
     5701 gaggacggtg gcgatcgcct ctccgattcc ccgtgaagcc ccggtgacca gcgcagtcct
     5761 tccggccagg ggccgttccc agtcgagttc cggcttcgga gcgaccggct tgccgacccg
     5821 gatcacctgc ccggaaacga aagccgaacg gggggaaagc aggaagcgca gggtggactc
     5881 gatttcgtcc ccggcgccgg gctcgacctg caccaactgg gcggtggaac cacggccacc
     5941 gatctccttg ccgagcgagc ggacaaaccc ttcaagtgca cgctgggcgg tgtgggcacg
     6001 gacgccgccg gtcgcggcgg gtggtgagcc gagcacgatc gcccggccac tcggtgcgag
     6061 ccgtggcacg gcggggtaga agaagcgctg cagttcgacc agctcggtcg agtcgctgat
     6121 tccggtggca tcgaagatga gtgctttc
//
LOCUS       DAOQMF010000012         5883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_5914_length_5883_cov_2.951441, whole genome shotgun sequence.
ACCESSION   DAOQMF010000012 DAOQMF010000000
VERSION     DAOQMF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5914_length_5883_cov_2.951441"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /gene="ffh"
                     /locus_tag="PLV77_00450"
     CDS             <1..1199
                     /gene="ffh"
                     /locus_tag="PLV77_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNC14350.1"
                     /translation="ERCLGADVLGSLNPGQQVVKIVNEELTDLMGGAGRDLVFSNKGI
                     TVILMAGLQGSGKTTATAKLAMLLKKEGKSVALAACDVYRPAAIKQLETVGKRAGAVV
                     YQQGTDADPVEIAAWALDKAKEEGRDVLIVDTAGRLHIDSELMDELARIRKRVKPHNV
                     LLVVDAMTGQDAVGVAESFSEAAEFDGVIISKLDGDARGGAALSVKAVTGKPIIFAST
                     GEKLEDFEHFHPDRMSSRILGMGDVLSLIEKAEEQVSEEEAEELQRKMSEGQFTLEDF
                     LKQMRQIRKMGPIGNLLGMMPGMGAMKNLKDVDVDDGELDRVEAIILSMTPHERAMPQ
                     IIKGSRRRRIAEGSGTKIQQVNNLVKQFDQMKKLMKHMANGQMPDQNQMAKMMGQTQR
                     PKIRRR"
     gene            1256..1510
                     /gene="rpsP"
                     /locus_tag="PLV77_00455"
     CDS             1256..1510
                     /gene="rpsP"
                     /locus_tag="PLV77_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNC14351.1"
                     /translation="MAVRIRLRRVGSKKNPIWRIVVADKRAPRDGRTIETIGQYNAQT
                     DPSTIVIDEERANYWLEKGAQPSERVAGLLRIKGIKATGS"
     gene            1525..1755
                     /locus_tag="PLV77_00460"
     CDS             1525..1755
                     /locus_tag="PLV77_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNC14352.1"
                     /translation="MQEMLLYITRALVEDPDAVEVEELEEDGDLIFEITVGEDDLGRV
                     IGRGGRVANAIRTIAKAAAVREERRVLVDILD"
     gene            1799..2458
                     /gene="trmD"
                     /locus_tag="PLV77_00465"
     CDS             1799..2458
                     /gene="trmD"
                     /locus_tag="PLV77_00465"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNC14353.1"
                     /translation="MNLDIFTLFPEAFDWFRQQRPVRKAIEHGAELNLRDYRDWTPLK
                     GGRVDDAPYGGGAGMVLRVDVVDAALRGVYGDGERPRTVVLSPAGRVLDDGLVTELAA
                     EPGLALLCGRYEGFDHRVHEHLADDEISIGRYVLAGGELPAMVLADTIMRRQPGALGD
                     EESAIEESWSEALEGLPEYPHYTRPPEYRGWGIPDVLISGDHAKVREWRLEQSRKRGA
                     E"
     gene            2527..2868
                     /gene="rplS"
                     /locus_tag="PLV77_00470"
                     /pseudo
     CDS             2527..2868
                     /gene="rplS"
                     /locus_tag="PLV77_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            3285..3866
                     /gene="lepB"
                     /locus_tag="PLV77_00475"
     CDS             3285..3866
                     /gene="lepB"
                     /locus_tag="PLV77_00475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNC14354.1"
                     /translation="MVVIVALALGLALLIQAFLIKPYQIPSGSMEPTLDINQRILVNR
                     FVYHFTDPKPGDVVVFHPPAGVDRGGLEECGAPHQQDQPCPQPTSDKSSETFIKRIVA
                     GPGDTVSIEKGLPVVNGEVVEPDGYEINPCGPAGTGCNMPKEITVPDGHFFMMGDNRG
                     HSDDSRFWGPLPEEWLIGKAFATYWPPNRIGGL"
     gene            3856..4518
                     /locus_tag="PLV77_00480"
     CDS             3856..4518
                     /locus_tag="PLV77_00480"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNC14355.1"
                     /translation="MGSSTARTPAAEQAPGELFSHDLELGASAVAGADEAGRGCLAGP
                     IVAAAVLFDRECLESQGGGRLAEIRDSKKLTARARERLYPEVLDCAVKTVVVMRSARY
                     IDANGLHVSNMECLARAIEGLGSGPEAVTLVDGFALKGCEVPHRRLVKGDSTSAAIAA
                     ASILAKVTRDRCMARAGLAYDGYGFEAHKGYASPAHRDAIRLLGPSPIHRLSFASEAY
                     QA"
     gene            complement(4556..4930)
                     /locus_tag="PLV77_00485"
     CDS             complement(4556..4930)
                     /locus_tag="PLV77_00485"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNC14356.1"
                     /translation="MDDRRATGRSAEDICARRLRERGWRILARNWRIRPGELDLIALA
                     GSTLVIVEVKAHHQGLRRGPVHPVLAVGPNKQRRIRRLASAWIAGHGRSIGFREVRFD
                     VVGITFDREGRLAAYDHLEDAF"
     gene            5094..>5883
                     /locus_tag="PLV77_00490"
     CDS             5094..>5883
                     /locus_tag="PLV77_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNC14357.1"
                     /translation="MLAETTSFTLDGIAARMIRVEVDVHRGLPNFQIVGLPDAAVREA
                     RERVRAATVNCGFDFPLQRITASLAPADLRKAGPAMDLALATALLGAAGDLKIEPLAG
                     WSLVGELALDGSVRPVRGALAMAEEAREQGRRGVIVPTANGPEAALAEGITVLTIDNF
                     GELASLQRGEIPGRLAIPAVPGGPEEMPDLADLRGQGHLRAAVEISAAGGHGLLMIGP
                     PGAGKSLAARRLPSVLPPLAGEEVLEVARIASACGIGDGRISSTR"
BASE COUNT         1071 a         1917 c         1998 g          897 t
ORIGIN      
        1 aggaacgctg cctcggcgcc gacgtgcttg gctcgctcaa cccgggccag caggtcgtca
       61 agatcgtcaa cgaggagctg accgacctga tgggcggggc cgggcgcgac ctcgtcttct
      121 cgaacaaggg gatcacggtc atcctgatgg ccggcctgca gggttccggc aagaccaccg
      181 ccaccgccaa gctggcgatg ctgctgaaga aggaaggcaa gagcgtcgcg ctggccgcct
      241 gcgacgtcta ccggccggcc gcgatcaagc agctggaaac cgtcggtaaa cgggccggcg
      301 cggtcgtcta ccagcaggga accgacgccg atccggtcga gatcgccgcc tgggcgctcg
      361 acaaggcgaa ggaggaaggc cgcgacgtcc tgatcgtcga taccgccggc cgcctccaca
      421 tcgactccga attgatggac gagctggccc gcatccgcaa gcgggtcaag cctcacaacg
      481 tgctgctggt ggtcgacgcg atgaccggtc aggacgcggt cggggtggcc gaatcgttca
      541 gcgaggcagc cgagttcgac ggggtgatca tctccaagct cgatggagac gcccgcggcg
      601 gcgccgccct atcggtcaag gcggtcaccg gcaagccgat catcttcgcc tcgaccggcg
      661 agaagctcga ggacttcgag catttccatc ccgaccggat gtccagccgg atcctcggca
      721 tgggcgacgt gctcagcctg atcgaaaagg ccgaggagca ggtcagcgaa gaggaagccg
      781 aagagctgca gcggaagatg tcggaggggc agttcaccct tgaggacttc ctcaagcaga
      841 tgcgccagat ccgcaagatg ggaccgatcg gaaacctgct cgggatgatg cccggcatgg
      901 gggcgatgaa gaatctgaag gacgtcgatg tcgatgacgg agagctcgac cgggtcgagg
      961 cgatcatcct ctcgatgacc ccgcacgagc gggcgatgcc gcagatcatc aagggttcgc
     1021 gccgccgccg gatcgccgaa ggctccggga ccaagatcca gcaggtcaac aacctggtca
     1081 agcagttcga ccagatgaag aagctgatga agcacatggc caacggccag atgccggacc
     1141 agaaccagat ggcgaagatg atgggccaga cccagcgacc gaaaatccgg cgccgctagc
     1201 caagttgccc gccacacgac tgtaagcccg ccaaacctgt acgattccgc gtccaatggc
     1261 agtccgcatc agacttagaa gagttggttc gaagaagaac ccgatctggc gaatcgtcgt
     1321 cgccgacaag cgtgccccgc gtgacggtcg caccatcgag acgatcggcc agtacaacgc
     1381 ccagaccgat ccgtcgacca tcgtgatcga cgaggaacgg gccaattact ggctcgaaaa
     1441 gggcgcccag ccctcggagc gggtcgccgg cctgctgcgc atcaagggca tcaaggccac
     1501 cggcagctga ggctcttccc gccgatgcag gagatgcttc tctacatcac gcgggccctg
     1561 gtcgaggatc ccgacgcagt cgaggtcgag gaactcgagg aagacggcga cctgatcttc
     1621 gagatcacgg tcggcgaaga cgatctcggc cgggtgatcg gccggggtgg cagggtcgcc
     1681 aacgcgatcc gcaccatcgc caaggccgcc gccgtgcgcg aagagcgccg cgtgctggtc
     1741 gacatcctgg actgacaccg atgcggtccg gccgcatacc cggcccggaa gcaggtcgat
     1801 gaatctcgac atcttcaccc tctttcccga ggcctttgac tggtttcggc agcagcggcc
     1861 ggtccgcaag gcgatcgagc acggcgcgga gctgaacctt cgggattacc gcgactggac
     1921 tccgctgaag ggtggccggg tcgatgacgc cccttacgga ggcggggcag gaatggtatt
     1981 gcgggtcgac gtggtcgacg ccgccctccg gggggtctac ggtgacggcg aaaggcccag
     2041 gaccgtggtt ctcagcccgg ccggccgggt actcgacgat ggcctggtca ccgaactcgc
     2101 ggccgaaccg ggccttgccc tgctctgcgg tcgctacgag ggtttcgacc accgggtcca
     2161 cgaacacctg gccgacgacg agatttcgat cggccgctac gtactggccg gtggcgagct
     2221 gccggccatg gtgcttgccg acacgatcat gcggcgccag cccggcgccc tcggcgacga
     2281 ggaatcggcg atcgaggaat cctggtccga ggccctcgag gggctgcccg aatacccgca
     2341 ctacacccgt ccacccgagt accggggctg gggcatcccc gacgtgttga tctccggcga
     2401 tcacgcaaaa gtcagggagt ggcgactcga gcaaagccgc aagcgggggg cggaatgacg
     2461 ctgcctgctc tgcttcggta ggattgcgcg tccgcgggcc ttcaaccggg tccgcttttt
     2521 tgagccatga gcaccgtaat ccaggacatt gagaacagtc agctgaggaa gggacttccg
     2581 tccttttccc cgggagatcg cgttcgcgtt cacttcctcg tcattgaggg aaaccgcaag
     2641 cgcccgcagg tattcgaggg cgtggtgatc aagcgtcagg gctccggtgc ccgcgagact
     2701 ttcactgttc gcaagcagtc cttcggcgtc ggtgtcgagc gtactttccc gctccactcg
     2761 ccgaagatcg agaagcttga agtcgcctcg cgtggccggg tccgccgggc caagctctac
     2821 tacctgcgcg gtcgggtcgg caaggccgcc cgggttgccg agcgtcgctg gggcattgac
     2881 gaggacatgg tcggcacatc caaggccgtg gacgcttcgg gtgaatccct cgaggagatc
     2941 gaggaagcga ccgaagtaga ggccacggag tcggacgctg ctcaggcaga agccacggaa
     3001 accgccgaag cgaccgaaac cgccgaagcg accggggccg aagctccgga agctcccgcc
     3061 gacgagccgg ccgcggagga agctccggcc ggggaagccg aagaggctcc cgccgaggag
     3121 cccgctgccg aggccgatga ggcgccggaa gccgaagctg ccgaagaggc tcctgaggcc
     3181 gaagcaactg aagagcccgc cgaagacggc gagcagggcg agaaggaaga agcctgagcc
     3241 cgaagatccc caagccgaaa acggccgggg gtagcttcgt cgaactggtc gtaatcgttg
     3301 cgctcgccct cgggctggcg ctgctgatcc aggccttcct gatcaagccg taccagatcc
     3361 cgtctggctc gatggagccg accctggaca tcaaccagcg gatcctggtc aaccgcttcg
     3421 tctaccactt caccgacccc aagccgggcg atgtcgtcgt ctttcatccg ccagccggag
     3481 tcgaccgggg cggcctcgag gaatgtggcg ccccacatca gcaggaccag ccctgtcctc
     3541 agccgacctc cgacaagtcg agcgagacct tcatcaaaag aatcgtcgcc ggtccggggg
     3601 acacggtctc gattgaaaag ggtttgcccg tcgtcaacgg cgaggtggtc gagccggacg
     3661 gctacgagat caacccctgc gggccggccg gcacaggctg caacatgccg aaggaaatca
     3721 ccgttcccga cggtcacttc ttcatgatgg gcgacaaccg gggacacagc gacgacagtc
     3781 gcttctgggg gcccctgccc gaagagtggc tgatcggcaa ggccttcgcc acctactggc
     3841 cgccgaaccg aatcggtggg ctctagcacc gcccgaactc ccgcggcgga acaggctccg
     3901 ggggagctgt tcagtcacga cctcgaactg ggtgcttccg ccgtcgccgg ggccgacgag
     3961 gccggccggg gttgccttgc gggcccgatc gtggccgcgg cggtcctttt cgaccgcgaa
     4021 tgcctcgaga gccagggcgg cgggcggctg gcggagatcc gtgactcgaa gaagctgacc
     4081 gccagggccc gcgagcggct ctaccccgaa gtgctggact gcgccgtgaa gacggtcgtg
     4141 gtgatgcgct cggccaggta catcgacgcc aacggacttc acgtaagcaa catggaatgc
     4201 ctggcccggg cgatcgaagg gctgggctcc ggacccgaag cggtgaccct ggtcgacggg
     4261 ttcgccctca agggttgcga ggtgccgcac cggcggctcg tcaagggcga cagcaccagc
     4321 gccgcgatcg cggccgcgtc gatccttgcc aaggtcaccc gtgaccgctg catggcgcgg
     4381 gctggcctgg cctacgacgg ctacggcttc gaagcccaca aggggtacgc atcacccgct
     4441 caccgggacg cgatcaggct gctcggaccc tcgccgatcc accggctctc cttcgcttcc
     4501 gaggcgtatc aggcctgacc ggcagcgcga tcgccccggg gctcgccgct tcacctcaga
     4561 atgcgtcttc aaggtggtcg taggcggcca gcctgccttc gcggtcaaag gtgattccga
     4621 ccacgtcgaa gcgaacttcg cggaacccga tgcttcgccc gtggccggca atccaggccg
     4681 atgccagccg cctgattcgc ctctgcttgt tggggcccac cgccaggacc ggatggaccg
     4741 gcccccgccg cagcccctga tggtgggcct tgacctcgac gatgaccagg gtggaaccgg
     4801 caagggcgat caggtcgagc tcgcccggcc ggattcgcca gttgcgggcc aggatccgcc
     4861 agccccgttc ccggagtctg cgggcgcaga tgtcttcggc gcttcgtcct gtggctcgtc
     4921 ggtcgtccat ccttcgacag tagggccggg accggcgatc tgcggctgaa aacggtcttt
     4981 tgttgcagtg aattcacacc ggtgtgagca ttgccgtccg gaattcacgg aggcgtgcgg
     5041 aatggttgca cccgtcgagc cacatgtgag tgaattcggc ctagcttggc cccatgctgg
     5101 ccgagaccac ctcattcacc ctcgacggga tcgccgcccg gatgatccgg gtggaggtcg
     5161 acgtgcaccg cggtctgccg aacttccaga tcgtcggcct tcccgacgcg gcggtccgcg
     5221 aagcacgcga gagggtccgg gccgcaacgg tcaactgcgg cttcgatttt cccctgcagc
     5281 ggatcaccgc cagcctcgcg ccggccgacc tgaggaaggc cgggccggcg atggatcttg
     5341 ccctcgcgac ggccctgctc ggcgctgcgg gtgatctcaa gatcgaaccg ctggcgggct
     5401 ggtcactggt tggtgaactc gccctggacg gttcggttcg cccggtccgg ggcgctttgg
     5461 cgatggccga ggaggctcgc gagcagggcc gacggggagt gatcgtcccg actgccaacg
     5521 gtcccgaagc cgcactggcg gaggggatca cggtcctgac gatcgacaat ttcggtgagc
     5581 tcgccagcct ccaacgaggg gagatcccgg gaagactggc gatccccgcc gtgccgggag
     5641 ggcccgaaga gatgcccgac cttgctgacc tgcgtggcca ggggcatctg cgagccgcgg
     5701 tcgaaatctc ggcggccggc ggtcacgggc tgctgatgat cggcccgccg ggcgcgggga
     5761 agtcgctggc ggcccggcgc ctgccctcgg tgctgccgcc actggctggc gaagaggttc
     5821 tcgaggtcgc aaggatcgcc agcgcctgcg ggatcggtga tggccggatc agctcgacca
     5881 ggc
//
LOCUS       DAOQMF010000013         5862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_5943_length_5862_cov_3.782676, whole genome shotgun sequence.
ACCESSION   DAOQMF010000013 DAOQMF010000000
VERSION     DAOQMF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5943_length_5862_cov_3.782676"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLV77_00495"
     CDS             <1..499
                     /locus_tag="PLV77_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNC14358.1"
                     /translation="DEAVADLAGREESAGEPEPVRFDIGVNAYLPASYVPFEAAKIDI
                     HRRIAAARRPGDLRQITDELIDRFGPLPEDVENLITLQQARIDLGSAGVEQVQFRGGK
                     LTVSGIELDSEAAAAIRESVPGALYEWRSHELSVRIDNDPAKRLATVSELGRALADVG
                     MLRQS"
     gene            543..1817
                     /locus_tag="PLV77_00500"
     CDS             543..1817
                     /locus_tag="PLV77_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC14359.1"
                     /translation="MSGARKFGLILFGAILVVLFAGIAIAQGVSKPGVESGDIITVQD
                     VPDGKGSITEEDYNRSFEQTWKRGGLQAAPKEGDAQYDQVKEAAINDLLDQAWLAGEA
                     NELGVTATDREIDNELATIKKDQFPTPKAFNDFLKSSGFTMAEVRDRVELQVLSRKIQ
                     DQITKSVTDVPDSEIENFYESSKESFTTPETRDIRLIVTDKKADAEQAISELGDDPTD
                     EEFAKVAKKLSVHSSKTEGGKTVATEGAFPDPAGADIMSAEAGTIEGPIESGKQFYVF
                     RVTKITPEETKPLDDVRDQIKQQLLPTLQQQAMSDFVSDYNSKWKSRTFCASDYTVER
                     CDNFESDGRLESADPKCYEAGAGKNPDLACPAPIELPKPMAPGGNSSPGALLGQTQNL
                     PQRPVQPSDAESDAAGAGGAGALSQLGAAAGQ"
     gene            1850..2500
                     /locus_tag="PLV77_00505"
     CDS             1850..2500
                     /locus_tag="PLV77_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNC14360.1"
                     /translation="MESGELAGALERLDRVTRELREKCPWDREQDARSIVPHTVEEAF
                     ELAEAAREGDDPAMRDELGDVLFQVVFLSLLMEERGEGDLARIANQLTDKLIRRHPHV
                     YPPEQSGRSEGIETADDVRRQWDEIKRDVEGRKSEKDWKKPALPALIYANKVQWKLDP
                     EERPANAGAANPDREQAEAEIGRLLWEAVAEARSRGVDPELALRAEAERHAETLQK"
     gene            2560..3852
                     /gene="eno"
                     /locus_tag="PLV77_00510"
     CDS             2560..3852
                     /gene="eno"
                     /locus_tag="PLV77_00510"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNC14361.1"
                     /translation="MSTIEAVHARQILDSRGNPTIEVEVTLENDFTGRAAVPSGASTG
                     EFEAVELRDGGDAYLGKSVNQAVANVNGEIREALLGFDVTDQAGIDKLMIDLDGTENK
                     GRLGANAILGVSLAAAHAAAAMEKRPLYSYLGDLYGESDPSLLPVPMMNVLNGGAHAD
                     NSVDFQEFMVMPAGTSSFSECLQVGTEVFHSLKKILSGHGLATAVGDEGGFAPDLSSN
                     EAALEMLIEGIEAAGYKPGEEVFIALDPATSEIYSDGVYRLEHEDRDLSPQQMADYWQ
                     SAAERFPIVSIEDGMDEEDWDGWKLLTEKLGDSVQLVGDDLFVTNPARLKRGIDLGVG
                     NSILVKVNQIGTLTETLEAIRMAADAGYSAVISHRSGETEDTTIADLAVATGAGQIKT
                     GAPSRSDRVAKYNRLLRIEEDLGERARFGGVSVLKRGN"
     gene            3892..4263
                     /locus_tag="PLV77_00515"
     CDS             3892..4263
                     /locus_tag="PLV77_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14362.1"
                     /translation="MRAHTYGDARRPPRRRPAPRRNAGKPGAGRIQWDRFGRIVLAIV
                     IVLLFAAMIKPLWNAVHTYRLAGQTKVRLHQAQAEQAMLQRQLRRASSESTLQREARR
                     LGMIRPEESAWVVNGTKGDSR"
     gene            4289..>5862
                     /locus_tag="PLV77_00520"
     CDS             4289..>5862
                     /locus_tag="PLV77_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14363.1"
                     /translation="MSFADFIAGLVHLALVAGFTGFTAFRLRARLLPGWGGAPARLVE
                     AVAGVGLLVLAAELLGLFGWLEILPLEVLLGLIAVSAWLRLVPRRPGTGDSVPPSPAV
                     STGAQVLAFLAAFAVFAQWGAFTSYNLDHGITNFDSVWYHMPFAAEIARTGSVTGFFH
                     PETVFLNWFYPQNSELVHAVAIVLTGRDFVSIFINLGWLALGLLAGWCVGRPYGRPQL
                     TMLGVAVLMATHTLVVREPGTGKNDVMAAALILASVAIMLNRSTSRPDPPGRVSPDWA
                     MAAGGLAIGLAAGTKVTALAPAFLVTCAVIYATIPGRRLRASGVWLGAAFIGGGWWYL
                     RNLFAAGNPMPQITRIGPLDLPAPDRLQVGRSDFSVFHYLTDTAVWKDYFVPGLDQGF
                     GHVWPLLFALVVGGLFLVMFTAHGRLTRTHGAVALLAILAYLVTPLSAAGPEGDPSAF
                     AINLRFLIPALGLAVVLIPLSSWFDRGWRRLALGLILIGLFIGTSATDPVISAPGRNF
                     GVAIALFFVALPFAFWL"
BASE COUNT          991 a         1997 c         1941 g          933 t
ORIGIN      
        1 cgacgaggcg gtggctgacc tggccgggcg ggaagagtcg gccggggagc cggaaccggt
       61 tcgcttcgac atcggcgtca acgcctacct gccagccagc tacgtgcctt tcgaagcggc
      121 caagatcgac atccaccgcc ggatcgccgc tgcccgccgc ccgggagacc tgcgccagat
      181 caccgacgag ctgatcgacc ggttcggtcc cctgccggaa gacgtcgaga acctgatcac
      241 gctccagcag gcccggatcg acctcggctc ggccggggtg gagcaggtcc agttccgcgg
      301 tggcaagctc acggtctccg gaatcgagct cgattccgag gcggcggccg cgatccgcga
      361 atcagtgccc ggagcgctct acgaatggcg ttcccatgaa ctctcggtcc gaatcgacaa
      421 cgaccccgcc aaacggctcg caacggtgtc cgaactgggc cgcgcactgg ccgatgtggg
      481 tatgctccgc cagtcgtagt ttcagcgacc agccacatca cctttccagg aggagccact
      541 gcatgagcgg cgcacgcaaa ttcggtctga ttcttttcgg agccatcttg gtggttctct
      601 ttgcgggtat cgcaattgca caaggcgtca gcaagcccgg cgttgaaagt ggcgacatca
      661 tcaccgtgca ggacgtgccc gacgggaagg gcagcatcac ggaggaggat tacaaccggt
      721 ccttcgaaca gacctggaag cgcggcggcc tgcaggcggc accgaaggag ggcgacgccc
      781 agtacgacca ggtcaaggaa gcggcgatca acgacctgct tgaccaggcc tggctggccg
      841 gcgaggcaaa cgaactcggc gtgaccgcca ccgatcgtga aatcgacaac gaactcgcga
      901 cgatcaagaa ggaccagttc ccgacgccca aggcattcaa tgacttcctc aagtccagcg
      961 gcttcacgat ggccgaagtt cgcgaccggg tcgaactcca ggtcctgagc cgcaagatcc
     1021 aggaccagat caccaagtcc gtcaccgatg tcccggactc ggagatcgag aacttctacg
     1081 aatcctcgaa ggagagcttc accacgccgg agacccggga cattcgcctg atcgtcaccg
     1141 acaagaaggc cgatgccgag caggcgatca gcgagctggg tgacgacccc acggacgagg
     1201 aattcgccaa ggtcgccaag aagctttcgg tccactcgag caagaccgag ggcggaaaga
     1261 ccgtcgccac tgagggtgct ttccccgacc cggccggcgc cgacatcatg agcgccgagg
     1321 cagggacgat cgaaggtccg atcgagtccg gcaagcagtt ctacgttttc cgcgtcacca
     1381 agatcacgcc ggaagaaacc aagccgctcg acgatgtccg cgatcagatc aagcagcagc
     1441 tgctgccgac cctgcagcag caggcgatga gcgacttcgt gtccgactac aactcgaagt
     1501 ggaagtcccg caccttctgt gcgtccgact acacggtcga gcgttgcgac aacttcgaaa
     1561 gcgacggtcg cctcgagtcc gccgatccca agtgctacga ggccggtgcc ggcaagaacc
     1621 ccgatctggc ctgcccggcc ccgatcgagc tgccgaagcc gatggccccg ggtggcaact
     1681 catcgcccgg tgccctgctc ggccagaccc agaacctgcc gcagcggccg gtccagcctt
     1741 ccgacgcgga gtctgacgcg gccggggccg gcggggcggg cgctctgagc cagctcggcg
     1801 ccgcggccgg ccagtagcaa gccgcggacc ggccggcagg cgggtggcaa tggaatccgg
     1861 cgaactcgcc ggagccctcg agcggctcga ccgggtaacc cgggaactcc gcgagaagtg
     1921 cccctgggat cgcgagcagg atgcccgctc gatcgtcccc catacggtcg aggaggcctt
     1981 tgagctggcc gaggcggccc gggagggcga tgacccggcg atgcgggatg aactcgggga
     2041 cgtgctcttc caggtcgtgt tcctttccct gctgatggaa gaacggggtg aaggtgacct
     2101 ggcccggatc gccaatcagc tcaccgacaa gctgatccgc cggcatccgc atgtctaccc
     2161 gccggagcag agcggcagga gcgagggcat cgagaccgcc gacgacgtcc gccgccagtg
     2221 ggacgagatc aagcgggatg tcgaaggcag gaagtccgag aaggactgga agaagccggc
     2281 cctgccggcc ctgatctatg cgaacaaggt ccagtggaag cttgacccgg aggagcgccc
     2341 cgcgaatgcc ggtgcggcca acccggatcg tgaacaggcc gaagctgaaa tcggacggct
     2401 gctctgggaa gcggtggccg aggccaggag ccgcggggtg gacccggaac tggctttgag
     2461 ggcggaggcc gaaaggcacg ccgagactct ccagaagtag gcgcccgagg cccgggccgg
     2521 gggcggagcc cgggcaacaa tctctagaat cggcggctaa tgagcacgat cgaagccgtc
     2581 cacgcacgcc agatcctcga ctcccggggc aaccccacga tcgaggtcga ggtcactctc
     2641 gaaaacgact tcaccggccg cgccgccgtt ccctccggcg cctcgacggg tgagtttgaa
     2701 gcggtggagc tccgcgatgg cggtgatgcc tacctcggca agtcggtcaa ccaggcggtg
     2761 gccaacgtca acggcgagat ccgcgaggcc ctgctcggct tcgacgttac cgatcaggcc
     2821 ggcatcgaca agctgatgat cgacctcgac ggaaccgaga acaagggccg gctcggcgcc
     2881 aacgcgatcc tcggcgtctc cctggccgct gcccatgccg ccgcggcgat ggagaagcgc
     2941 ccgctttaca gctacctcgg cgatctctac ggcgagtcgg acccgtcgct cctgccggtg
     3001 ccgatgatga acgtcctcaa cggcggcgcc cacgccgaca attcggtcga tttccaggaa
     3061 ttcatggtca tgccggccgg cacctcgagc ttctccgaat gcctgcaggt ggggaccgag
     3121 gtgttccact cgctgaagaa gatcctctcc gggcacgggc tggccacggc ggtcggtgac
     3181 gaaggcggct tcgccccgga cctctcctcg aacgaggcgg cgctcgagat gctgatcgaa
     3241 ggcatcgagg cagccggtta caagcccggc gaagaggtct tcatcgcgct cgacccggcc
     3301 acctccgaga tctactcgga tggcgtctac cggctcgaac acgaagaccg cgatctcagc
     3361 ccgcagcaga tggccgatta ctggcagtcg gcagcggagc gcttcccgat cgtctcgatc
     3421 gaggacggca tggacgagga ggactgggac ggctggaagc tgctgaccga aaagctcggc
     3481 gattcggtcc agctggtcgg cgatgacctg ttcgtgacca acccggcccg tttgaagcgc
     3541 ggaatcgacc tgggcgtcgg caactcgatc ctggtcaagg tcaaccagat cggcaccctg
     3601 accgaaaccc tggaggcgat ccgcatggcc gccgacgccg gctactccgc ggtgatctcc
     3661 caccgttccg gtgagaccga ggacaccacg atcgccgatc tcgccgtggc gacgggggcc
     3721 ggccagatca agaccggggc gccctcccgg tcggaccggg tcgccaagta caaccgcctg
     3781 ctccggattg aggaagacct gggcgaacgg gcccgtttcg gcggcgtatc ggttctgaag
     3841 cgagggaatt gaaggaattg accgtccggc ctagaagggt cggacgtgtc ggtgcgcgcg
     3901 catacctatg gcgatgcccg gcggccgcca cgccgccggc ctgcccctcg ccggaacgcc
     3961 ggcaagccgg gcgcgggccg gatccagtgg gaccgcttcg gccgcatcgt gctggcgatc
     4021 gtgatcgttc tcctgttcgc cgcgatgatc aagccactgt ggaacgccgt tcacacctac
     4081 cggctggccg ggcaaaccaa ggtgcggctg caccaggccc aggccgagca ggcgatgctg
     4141 cagcgtcagc tccggcgggc cagcagcgag tccacgctcc agcgcgaggc gcggcgactg
     4201 gggatgatca ggccggagga gtcggcctgg gtcgtgaacg gcaccaaggg cgactcccgg
     4261 tagggagtgg cgaggccggt ggcctcaatt gagtttcgct gacttcatcg ccgggctcgt
     4321 tcacctggcc ctggtggcgg gcttcaccgg tttcaccgcg ttccggctca gggcccgtct
     4381 gctgcccggg tggggcggcg cacctgcccg tctggtcgag gcggtggccg gggtcggcct
     4441 gctggtcctg gccgccgagc tgctcggcct cttcggctgg ctggaaatcc tgcccctgga
     4501 ggtcctgctc ggactgatcg cggtgtcggc atggctccgg ctcgttccgc ggcggcccgg
     4561 gaccggtgac tcggtcccgc catccccggc ggtttccacc ggagcccagg tcctcgcctt
     4621 cctggcggcc ttcgccgtct tcgcccagtg gggcgccttc acctcctaca acctcgacca
     4681 cggcatcacc aacttcgatt cggtctggta ccacatgccg ttcgcggccg agatcgcccg
     4741 caccgggtcg gtgaccggct tcttccatcc ggagaccgtc ttcctcaact ggttctaccc
     4801 ccagaactcg gaactcgtcc acgcggtcgc gatcgtcctg actggtcgcg acttcgtctc
     4861 gatcttcatc aacctcggct ggctggcgct tgggctgctg gccggctggt gcgtcggccg
     4921 cccctacggc cggccgcagc tgaccatgct cggggtggcc gtgctgatgg ccacccacac
     4981 cctggtcgtg cgggagccgg gcaccggcaa gaacgacgtt atggcggccg ccctgattct
     5041 cgcctccgtc gcgatcatgc tgaaccgctc gaccagccgt ccggatccgc cggggcgggt
     5101 ctcgccggac tgggcgatgg cggcgggggg actggcgatc ggcctcgccg cgggaaccaa
     5161 ggtgacggcc ctggccccgg ccttcctggt cacctgcgcg gtgatctatg cgacgatccc
     5221 gggccggcgt ctgcgggcca gcggagtctg gctcggcgcc gccttcattg gcggcggctg
     5281 gtggtacctg agaaacctgt tcgcggccgg caacccgatg ccgcagatca ccaggatcgg
     5341 cccgctcgac ctgccggctc ccgaccgcct ccaggtcggt cgctccgact tctcggtctt
     5401 tcattacctg accgacaccg cggtctggaa ggactacttc gtgcccggtc tcgatcaggg
     5461 ctttggccac gtgtggccgc tcctctttgc cctggtcgtg ggcggcctgt tcctggtcat
     5521 gttcaccgcc cacgggcgtc tgacccgcac ccacggcgcg gtcgccctgc tggccatcct
     5581 ggcctacctg gtcacgccgc tgagcgccgc tggaccggaa ggcgatccca gcgccttcgc
     5641 gatcaacctg cgcttcctga ttccggcact gggcctggcg gtggtcctga tcccgctttc
     5701 ctcgtggttt gaccgcggct ggcgccgcct tgccctgggc ctgatcctga tcgggctctt
     5761 catcggcacc agcgccaccg acccggtcat ctcggctccg ggccgcaact tcggggtggc
     5821 gatcgccctg ttcttcgtgg cgcttccctt cgccttctgg ct
//
LOCUS       DAOQMF010000014         5815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_6019_length_5815_cov_4.436979, whole genome shotgun sequence.
ACCESSION   DAOQMF010000014 DAOQMF010000000
VERSION     DAOQMF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6019_length_5815_cov_4.436979"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLV77_00525"
     CDS             <1..611
                     /locus_tag="PLV77_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC14364.1"
                     /translation="AKVKKHGIRASHTADVHLDDCRVPGSCLLGGKEKLDERLARVRE
                     GKKGKSQAAMATFEASRPIVGSQAIGIARAAYEYALEYAKERVQFGRPIIENQAIAFT
                     LADMCLEIDAARLLVWRASWMGRNQIPFKNSEGSMSKLKGGEVATWVTERAIQILGGN
                     GYTREFPVERMHRDAKIYDIFEGTAEIQRLVISRSISGIKIK"
     gene            complement(887..2281)
                     /locus_tag="PLV77_00530"
     CDS             complement(887..2281)
                     /locus_tag="PLV77_00530"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HNC14365.1"
                     /translation="MTPSDPTPQQKLNPEQQEMMVESFFTRPAADRKIPELGVIPRAA
                     YNMVASEMELDGDPNKNLATFVTTWMEPEARQVITDSLHKNYIDHAEYPRTAEISKRC
                     VRMIHDLFNGPEGQDAPGTACAGSSEAVMLGALSMKWNWKNRQEKAGKDATRPNLVYG
                     SDVHVVWDKFCRYFDVEPRNIPIPKGKTVMGPDDFREHIDENTIGVIGVVGTTFTGQC
                     DDVVGIDQMLTEFKGKGLEVPMHIDAASGGFVFPFSHPDFEWDFRLDSVVSINVSGHK
                     FGLVYPGIGWLITRTDAQVAEDLIFYEDYLGEKDATFTLNFSGSSSFVLAQYYQFLRL
                     GHSGYSSMVRAMTKNREALADRLRDMDALTVYEDDSPTLPLLIAKTNDNEPFDSNDLV
                     GELARRRGWLVPAYQMPPNNENDRIMRMLVKFNQTRELVDALCDDFEASISFLRKRGE
                     GKVDSPPAHTGHGY"
     gene            complement(2392..3264)
                     /locus_tag="PLV77_00535"
     CDS             complement(2392..3264)
                     /locus_tag="PLV77_00535"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNC14366.1"
                     /translation="MLIGAHVSTSGGLAKAVSRGEEIGCRSIQIFNQSPRAWKPTEYS
                     EEDFAEFREAMAASQVESVIIHAIYLINCVTPDRDLARKSRKALIHALRVGDGIGADG
                     VVLHPGSRKQEEVVPAIVRGGGLIEEALSESESCPLLIEQMAGHQNILAHTFEEIAGL
                     IEAAGGDRRLGLCLDSCHLFASGYDIGTYQGIDRMLKESDKAVGKRRLKALHLNDSKE
                     PLGSRRDRHEDIGKGEIGETGFARFLSEPRFEGLPATMETPGPDRKGPVAADIELAEK
                     LKRRGRAARRRRNS"
     gene            3257..4672
                     /gene="gltX"
                     /locus_tag="PLV77_00540"
     CDS             3257..4672
                     /gene="gltX"
                     /locus_tag="PLV77_00540"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNC14367.1"
                     /translation="MSIEKSGLRVRFAPSPTGALHIGGARTALYNWLAARHYGGDLVL
                     RIEDTDLERSTPENVAQILDALRWLELDWDEGPVSQVGRAEDHAAALQKLLDSGAAYR
                     DAATADDVKAWKSEHGADRGYRGTPSEDGSGAIRLRVPDDGDTVVNDLIRGEVRFPNR
                     SYDDFVIARGDGSVLYNFAVAVDDADMGITDVIRGDDHLSNTPKQLLVFEALGIQPPR
                     YAHVPLLHGPDGKKLSKRHGAASVQELRDAGYLPAAIRNYVALLGWGPGDDETLLSTA
                     ELIERFRPEDVGRSSAVFDEKKLRWMNGRYMRELPPGEYRESVRSFVEAETVGRPLNE
                     DRFAFGCEIVQEKAQTLQEVWPLIRFLFEPPVQDEKAWRKVMKGDAERVLVAVTEALQ
                     AVPAGGEGPNGDGFTIEAVQTALEPLPEKLELGAGKVFQPIRVSITGTTISPGIFESV
                     AALGREETIARIEAARSRLDA"
     gene            4752..5150
                     /locus_tag="PLV77_00545"
     CDS             4752..5150
                     /locus_tag="PLV77_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC14368.1"
                     /translation="MISVTSKSRYAVVALVELAGAGDRPMPVKELAERRGIPDQFLEQ
                     LFSTLRRSGLLTSRRGSKGGYTLSRPASDITVLEVVQALDGKVGQEADEAGGIWAEGV
                     RALRGVFGETTIQDLYEREAEDAAARMYFI"
     gene            5147..>5815
                     /locus_tag="PLV77_00550"
     CDS             5147..>5815
                     /locus_tag="PLV77_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC14369.1"
                     /translation="MIAPQASATVGGTPLVWLARIADGLDAQVCAKLEYFNPGGSVKD
                     RIGAAMIGAAEARGELVRGESVIVEPTSGNTGIALAMICAARGYQLVLTMPEGMSRER
                     SKLLRAYGAEVQETSSMGGMDEAVTLAEKIASQRDGYMPLQFSNPANPEAHFRSTGPE
                     IWTDTGGEVDAFVCGVGTGGTITGVARYLKDQGSDARIYAVEPASSPVLSGGRLGPHR
                     IQGIG"
BASE COUNT         1001 a         1849 c         1937 g         1028 t
ORIGIN      
        1 gcgcgaaggt caagaagcac ggcatccgcg cctcgcacac tgccgatgtt cacctcgacg
       61 actgccgtgt gccgggcagc tgcctgctcg gcggcaagga gaagctcgac gagcgtctcg
      121 cccgggttcg tgaaggcaag aagggcaagt cgcaggcagc gatggcgacc ttcgaggctt
      181 cccggccgat cgtcggctcg caggcgatcg gcatcgcccg tgccgcctac gagtacgccc
      241 tcgagtacgc caaggaacgc gtccagttcg gtcgtccgat catcgagaac caggcgatcg
      301 ccttcaccct cgccgacatg tgcctcgaga tcgatgcggc tcggctgctc gtctggcgcg
      361 cctcctggat gggtcgcaac cagatcccgt tcaagaactc cgaaggctcg atgtcgaagc
      421 tcaagggtgg cgaggtcgcg acctgggtca ccgagcgtgc gatccagatc ctcggcggca
      481 acggctacac ccgcgagttc ccggtcgagc ggatgcaccg cgacgccaag atctacgaca
      541 tcttcgaggg caccgcggaa atccagcgtc tcgtgatctc ccgcagcatc tcgggaatca
      601 agatcaagta ggggaaacga ggtgccgcag gtttacgacc gcagaggtca caaacctgcg
      661 gcacctcgga cttcaaaggc gggcgagggg aggcatctcc cgatctggag tagccgcggg
      721 gtcgggtggc cctcccaaag tgccttcccg attactgctc cgaccgtcga gcaggcactc
      781 gttcacgcga cggtagaggc atctttggga gggccacccg gccccacggc gtaaccaccc
      841 gctgccggag cgcggaagcg accccgcggg tcgcggatct ggtttctcag tagccgtggc
      901 cggtgtgggc cggggggcta tcgacctttc cttctccgcg cttgcgcagg aaggagatcg
      961 aggcttcgaa atcgtcgcag agcgcgtcga ccagttcacg ggtctggttg aacttgacca
     1021 acatgcgcat gatccggtcg ttctcgttgt tcgggggcat ctggtacgcc ggcacgagcc
     1081 agcctcgacg gcgggcgagt tctccgacca ggtcgttgct gtcgaacggc tcgttgtcat
     1141 tcgtcttcgc gatcagcagc ggcagggtcg ggctgtcgtc ctcgtagacg gtgagcgcat
     1201 ccatgtctcg gagccggtcg gcgagcgcct cgcggttttt ggtcatcgcc ctgaccatcg
     1261 agctgtagcc cgaatgaccg agccgcagga actggtagta ctgggcgagg acgaaggacg
     1321 agcttcccga gaagttcagg gtgaaggtgg cgtccttctc cccgaggtag tcctcgtaga
     1381 agatcaggtc ttcggccacc tgggcgtcgg tccgggtgat cagccagccg atcccggggt
     1441 agaccaggcc gaacttgtgg cccgagacgt tgattgaaac gaccgagtcg agccggaaat
     1501 cccactcgaa gtcggggtgc gagaacggga agacgaaccc gcccgaagcg gcgtcgatgt
     1561 gcatcggcac ctcgaggccc ttgcccttga actcggtcag catctggtcg atgccgacca
     1621 cgtcatcgca ctgaccggtg aaggtagtgc cgacgacgcc gatcaccccg atcgtgttct
     1681 cgtcgatgtg ctcgcggaag tcgtccggcc ccatgaccgt cttgcccttc ggaatgggga
     1741 tgtttcgggg ctcgacgtcg aagtagcggc agaacttgtc ccagaccacg tgaacgtccg
     1801 acccgtagac gaggttgggc cgggtcgcgt ccttgccggc cttctcctgg cggttcttcc
     1861 agttccactt catggaaagc gcgccgagca tgaccgcctc ggacgagccg gcacaggcgg
     1921 tgcccggagc atcctggcct tcgggaccgt tgaagaggtc gtgaatcatg cgcacgcatc
     1981 gcttggagat ctccgcggtg cgcgggtact cggcgtggtc gatgtagttc ttgtgcaggc
     2041 tgtcagtgat cacctggcgg gcttcgggct ccatccaggt ggtgacgaag gtcgccaggt
     2101 tcttgttcgg gtcaccgtcg agttccatct ccgaggcgac catgttgtag gcggcccggg
     2161 ggatgacacc aagctcggga atcttgcggt ccgcagcagg ccgggtgaag aagctctcga
     2221 ccatcatctc ctgctgttcg ggattcagct tctgctgggg tgtggggtcc gacggggtca
     2281 tctatctcct tcgacgatcg gtttcccaaa aacctatccg ctgcctgtca ctgtccgttc
     2341 atgccgatcg gatgatcccc gatccggcgc ggatgatcaa cggagcaggc cctacgaatt
     2401 gcggcgacgg cgagcggccc gtcccctccg cttcagcttt tcggcgagtt cgatgtcggc
     2461 ggcaaccggt cccttcctgt ccggccccgg ggtctccatc gttgcgggca ggccttcgaa
     2521 tctcggttcc gagaggaatc gggcgaatcc ggtttcgccg atctcgccct ttccgatgtc
     2581 ctcatgacgg tcgcggcggc tgccgagcgg ctccttcgaa tcattcaggt gaagagcctt
     2641 caggcgccgc ttgccgaccg ccttgtccga ctccttcagc atccggtcaa tgccttggta
     2701 ggtaccgatg tcgtaccccg acgcgaaaag atggcaggaa tccaggcaga gcccgagtcg
     2761 ccggtcgccg ccggccgcct cgatcaatcc ggcgatctcc tcgaaggtgt gggcgaggat
     2821 gttctggtgg ccggccatct gttcgatcaa gagcggacag ctttcgctct ccgaaagggc
     2881 ctcttcgatc aaaccgccgc cccggacgat cgccggcaca acctcttcct gcttgcgtga
     2941 accggggtgg aggaccacgc cgtcggcccc gattccgtcg ccgacccgca gcgcgtggat
     3001 cagcgccttg cgcgacttcc tggccaggtc acggtccggc gtgacgcagt tgatcaggta
     3061 gatcgcgtgg atgatcacgc tctcgacctg actcgcggcc atcgcctcgc ggaactcggc
     3121 gaagtcctcc tccgagtatt cggtcggctt ccaggcccgc ggactctggt tgaagatctg
     3181 gatcgagcgg cagccgatct cttccccccg ggacacggcc ttcgccaagc cgccggaagt
     3241 ggaaacatgg gcaccgatga gcattgagaa aagcggcctc cgggtcagat tcgcaccttc
     3301 tcccaccggc gcactacaca tcgggggagc cagaaccgca ctgtacaact ggctggccgc
     3361 cagacattac ggaggcgacc tcgtgctccg gatcgaggac accgacctcg agcggtcgac
     3421 cccggagaac gtggcccaga ttctcgatgc cctgcgctgg ctggagctcg actgggacga
     3481 aggtccggtg tcccaggtcg ggagggccga ggaccacgcc gcggcactcc agaagctgct
     3541 cgattcaggt gccgcgtacc gtgacgccgc caccgccgat gacgtcaagg cctggaagag
     3601 cgaacacggg gcggaccggg ggtaccgggg aaccccttcc gaagatggct ccggcgcgat
     3661 acggctgcgg gtgcccgatg acggtgacac cgtcgtcaat gacctgatcc ggggcgaagt
     3721 caggtttccc aaccgcagtt acgacgactt cgtgatcgcc cggggcgacg gttcggtgct
     3781 ctacaacttc gcggtcgcgg tcgatgacgc cgacatgggg atcaccgacg tgatccgcgg
     3841 ggacgatcac ctctcgaaca ccccgaagca actgctcgtc ttcgaagcgc tgggaatcca
     3901 gccgccccgc tacgcccacg tgcccctgct gcatggtccc gacggcaaga agctctcgaa
     3961 gcgccacggt gccgcctcgg tccaggagct tcgtgacgcg ggttacctgc cggcggcgat
     4021 ccgcaactac gtcgccctgc tgggctgggg gcccggcgac gacgaaaccc tgctctcgac
     4081 cgcggagctg atcgagcgat tccggcccga ggacgtgggc aggtcttcgg cggtcttcga
     4141 cgagaagaag ctccggtgga tgaacggccg ttacatgcgg gaactgccgc ccggcgaata
     4201 ccgggaatcg gtgcgctcct tcgtggaagc ggagaccgtc ggccgtccgc tgaacgagga
     4261 tcgcttcgcc ttcggctgcg agatcgtcca ggagaaggcg cagaccctgc aggaagtctg
     4321 gcccctgatc cggtttttgt tcgaaccacc ggtccaggac gaaaaggcct ggcgcaaggt
     4381 gatgaagggc gacgccgaaa gggtgttggt tgcggtcacg gaggcgcttc aagcggttcc
     4441 ggccggtggc gagggcccga acggagacgg cttcacgatc gaggcggtcc agacggcact
     4501 cgagccgctg ccggagaagc tcgaactggg agccggcaag gttttccagc cgatccgggt
     4561 ttcgatcacg ggaacgacca tctcgcccgg aatcttcgaa tcggttgccg ctctgggccg
     4621 ggaagagacc atcgcccgga tcgaagcggc ccgctcccga ctggatgcgt agtgccccat
     4681 tcctgacaaa tacctgtttc ctgaccgggt tactaagttt ttgacgggct gatgcggtaa
     4741 aaaggggagc ggtgatctcg gttacctcca aatcccgcta cgccgtggtc gctctggtcg
     4801 agctggccgg agccggtgac cggccgatgc cggtcaagga actggccgag cggcggggaa
     4861 tcccggatca gttcctcgaa cagctgtttt cgaccctgcg gcgctccggc ctcttgacca
     4921 gccgtcgcgg cagcaagggc ggctacaccc tttcccgccc ggccagtgac atcaccgtgc
     4981 tggaggtggt tcaggcgctg gacggcaagg tcgggcagga agcagacgag gccggtggca
     5041 tctgggccga aggagtcagg gccctgaggg gcgttttcgg cgagaccacg attcaggacc
     5101 tgtacgagcg ggaagccgaa gacgccgcgg cgaggatgta cttcatttga tcgccccgca
     5161 ggcatcggcg acggtcggcg ggactccgct ggtctggctg gcccggatcg cggacgggct
     5221 ggatgcccag gtctgcgcca agctcgagta cttcaatccg ggcggctcgg tcaaggaccg
     5281 gatcggcgcg gcgatgatcg gggccgccga ggcgagggga gagctggtcc gcggcgagtc
     5341 ggtgatcgtc gagccgacca gcggcaacac cgggatcgcc ctggcgatga tctgtgccgc
     5401 ccggggctac cagctggttt tgaccatgcc ggaaggcatg agtcgcgaac gcagcaagct
     5461 gcttcgcgcc tacggggccg aggttcagga aacgtcgtcg atgggcggca tggatgaagc
     5521 ggtgaccctg gccgagaaga tcgcctccca gcgcgatggc tacatgccac ttcagttctc
     5581 gaaccccgcc aatcccgagg ctcacttccg gtcgaccggt cccgagatct ggaccgacac
     5641 cggtggcgag gtcgatgcct tcgtctgcgg cgtgggaacc ggcggcacga tcaccggcgt
     5701 ggcccgttac ctgaaggacc agggttccga cgcccggatc tacgcggtcg agcccgcctc
     5761 gtctccggtg ctttccggcg gccgacttgg cccccaccgg atccagggga tcggg
//
LOCUS       DAOQMF010000015         5753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_6118_length_5753_cov_3.759916, whole genome shotgun sequence.
ACCESSION   DAOQMF010000015 DAOQMF010000000
VERSION     DAOQMF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6118_length_5753_cov_3.759916"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLV77_00555"
     CDS             complement(<1..185)
                     /locus_tag="PLV77_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC14370.1"
                     /translation="MVDFTLSDEQKDLREMAHNFAEKEMRSVAFEYDKDGTWPAEVIQ
                     KAWEVGLMNTHVDEAYGG"
     gene            326..754
                     /locus_tag="PLV77_00560"
     CDS             326..754
                     /locus_tag="PLV77_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14371.1"
                     /translation="MVAELSFVLILLCVVVVPVAAIAFARSGKAYEEIGKGRFAVDFD
                     DSSETDHHEELRQLVEAKAYRQARRGEQPVDVEREVERLSKGEPEETADAAPPPDPAT
                     AGIRAEIREVVIAKNESRERRGEEPLDVESEVERMLAEFG"
     gene            784..1116
                     /locus_tag="PLV77_00565"
     CDS             784..1116
                     /locus_tag="PLV77_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14372.1"
                     /translation="MDDSTSRYELEELVVQPGTYFNPQTEVVVVVDDSASIDQEIFNM
                     ESYEGADWVRIADDVAVDEQQRDELLEHFQTHYHPGNGGSVSGTALEQGDDELDEDEE
                     GQEIGRED"
     gene            1116..2000
                     /locus_tag="PLV77_00570"
     CDS             1116..2000
                     /locus_tag="PLV77_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNC14373.1"
                     /translation="MADRGPLTLLGVPIDSVGTAGGTELGPNVLRQLLGPEGFEDAGD
                     TSRKIRGLERDRANGWLAFDDILAMTGEVRGRVADLTAAGKVPVLLGGCCTLLPGALA
                     GARDSLGEFGLAYFDGHLDLFTGLTSPTGEGADFPVAASLGMAPDPLLEVIGETPVVA
                     PGRMSLVGARDKEELQLISPLPEGLGIGRIEFRDDLRHADLNEVGASLEAELTDGGSG
                     FWLHLDVDVLDREAFPATDYLMPDGLSMDELRALFAPLASSPGLIGVDVTCFNPEKDP
                     DERCGTELAELLRGTLNG"
     gene            2041..2520
                     /gene="bcp"
                     /locus_tag="PLV77_00575"
     CDS             2041..2520
                     /gene="bcp"
                     /locus_tag="PLV77_00575"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNC14374.1"
                     /translation="MLEEGDKAPDFTLEDQLGEKVKLSDHRKELVVLYFYPKASTSGC
                     TTQACSIRDNSADYAKAGATVIGISPDEVKDISRFATKNDLEFTLVADKDHKVAEKYG
                     TWVEKSMYGNKYMGVQRATFIIRGGKILRVFPKVQPKKHDGLVLGALAEINEENRDV"
     gene            2513..3301
                     /locus_tag="PLV77_00580"
     CDS             2513..3301
                     /locus_tag="PLV77_00580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC14375.1"
                     /translation="MSNWDKGSYEVTAEQIAPATPAVADAVEPVTGKLALDLACGTGN
                     EALELARRGARVTGYDGAPRLLQVAGERAAAEGLELERVQGDLAELPFDDDSFEIVTS
                     VFGLIFTGSPQDSAAEIGRVLRPQGRIAFTSWVEEGLFQYMQDMAKAAVASHFGQAPP
                     EGADAPFAWGDEVAVRELFSESGLMLQVETRELVIEEESAAALNDRWFDLHPIWITMQ
                     EVIGDEAYEEMRREALPVVESHNEVSDGSFRNTLRYLLFEGSPV"
     gene            complement(3503..4531)
                     /locus_tag="PLV77_00585"
     CDS             complement(3503..4531)
                     /locus_tag="PLV77_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNC14376.1"
                     /translation="MEYLLLLFSFAIILTGALLFTNAIEWLGHKLNLGEGAVGSILAA
                     VGTAMPETLIPIVAIIGGVSGSDEVAVGAIVGAPFLLATIAMALVGISALAYIKRRPQ
                     GKHLDVHVETLDRDLVFFMIFFTLALVVGVVDVPQSVQIGGGVICLLAYVYYVVQTIR
                     NSGDVAESHEMDPLIMDRRTDRTGGPPNSIMIIQLLVGLASMVGGAHLFVHELLSVAE
                     HLGVSAIILSLILAPLATELPEKANSFFWVRDGKDALALGNITGAMVFQSTIPVAIGM
                     IFIDWSLDKYAVLSMLLGLAGGVVAYWSLRRNGQFNKPSIAIWGLLYLTFLAFVILSG
                     DTGHSTAH"
     gene            complement(4550..5170)
                     /locus_tag="PLV77_00590"
     CDS             complement(4550..5170)
                     /locus_tag="PLV77_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14377.1"
                     /translation="MERTRIPLVLVLVLAFSLVMQACGSSDPDPATILDRALTREHLS
                     SFGNGSGGPTGGIVSVEALGYEDRVLDERKVAADPQVMSDIRSALGSDSGLRGLVGDL
                     RYDGTEEVSGVGTDHISGELDVAGLARALEAAGGGEVGQIAGVKTGSSLEDSLVSADF
                     DLYAGQQDGDIRRLDLTLALDDPDNALPPARIRFSLTPRPSGANQT"
     gene            5200..>5753
                     /locus_tag="PLV77_00595"
     CDS             5200..>5753
                     /locus_tag="PLV77_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNC14378.1"
                     /translation="MPPRTILYTGKGGVGKTSVAAATARRCAAEGRRTVILSTDPAHS
                     LSDSLGVELGPDPVEVSPGLWGQEVLAEAEMRRNWDRVAAWMSGLLQSKGVDQVRAEE
                     LSVPPGLDELFSLLQIKRHWDKSEFDVVIVDCAPSGETLRLLGYPEVASWWVRKVFPW
                     NRSLLSNAAPIAKALEILMPEPEL"
BASE COUNT         1071 a         1807 c         1903 g          972 t
ORIGIN      
        1 ccgccgtaag cctcgtccac atgggtgttc atgaggccga cttcccaggc cttctgaatc
       61 acttcggcag gccaggtgcc gtccttgtcg tattcgaagg cgacgcttcg catctccttc
      121 tcggcgaagt tatgcgccat ttcgcgcaga tccttctgct cgtcgctcag cgtgaaatca
      181 accactcttc aagctcctta ggtcggttgg aaccgttcga tttgctcgat gcggggccgg
      241 ggcgaggagg cccgggcggc tcccgattga cttgtcaatc aataactctt cgaacagggt
      301 accaagcgcc tttactcttg gggggatggt tgcggaactt tctttcgtcc tgatcctcct
      361 ttgcgtggtc gtggtgccgg tcgcggcgat cgccttcgcc cgctcgggca aggcgtacga
      421 agaaatcggg aagggacgat tcgcagtcga cttcgatgac agcagcgaga ccgaccatca
      481 cgaggagctc cgacagctgg tcgaggccaa ggcctaccgc caggcgagac ggggcgagca
      541 accggttgac gtcgagcggg aagtcgagcg gctctcgaaa ggagaaccgg aagaaacggc
      601 cgatgccgct ccgccgccgg atcccgcgac cgcaggaatt cgcgcggaaa tccgcgaggt
      661 ggtcattgcg aaaaacgaga gccgcgagcg ccggggcgag gaaccgcttg acgtcgaatc
      721 cgaagtcgag cggatgctgg ccgagttcgg ctagcggcga cgggtcgtgg gtagtattgg
      781 ctcatggatg actcgacttc ccgttacgaa cttgaagagc tggtggttca accggggacc
      841 tacttcaacc cgcagaccga agtcgttgtc gtggttgacg attcagcgtc gatcgaccag
      901 gagatcttca acatggagtc ctacgagggc gccgactggg tgaggatcgc cgacgatgtc
      961 gcggtcgacg agcagcaacg cgatgaactg ctcgagcatt tccagactca ctaccacccc
     1021 ggaaacggcg ggtcggtttc cggcaccgcc ctggaacagg gtgacgacga gctcgacgag
     1081 gacgaagaag gccaggaaat cggccgcgaa gactgatggc cgatcgcggg ccgctgaccc
     1141 tcctgggcgt cccgatcgac agcgtgggca cggccggagg taccgagctc ggaccgaacg
     1201 tcttgcggca actgctgggg cccgaagggt tcgaggacgc cggcgacacc agccgcaaga
     1261 tccgcggact ggagcgggac agggccaacg gctggctggc tttcgacgac atcctggcga
     1321 tgaccggcga ggtgaggggc cgggtcgcgg atttgaccgc cgccgggaag gtgccggtgc
     1381 tcctcggcgg ctgctgcacc cttttgccgg gcgcgctggc cggagcccgg gattcgctcg
     1441 gcgaattcgg tctcgcctac tttgacggcc atctcgacct tttcaccggg ctgacttcgc
     1501 cgaccggtga aggggccgac tttccggtcg ccgcatcgct cggaatggct cccgatcccc
     1561 tgctcgaggt gattggcgag acaccggtgg tcgcgcccgg gcggatgtct ctggtcggcg
     1621 caagagacaa ggaggagctc cagttgatct cgccgctgcc ggagggtctc ggcatcggcc
     1681 ggatcgagtt ccgcgacgat ctcaggcacg ccgacctgaa cgaagtcggc gcctcgctcg
     1741 aggccgaact gactgatggt ggctccggat tctggcttca cctcgacgtc gacgtcctcg
     1801 accgtgaagc cttccccgcg acggactacc tgatgcccga cgggttgagc atggatgagc
     1861 tccgcgccct gttcgcgccc ctggcttcct ctccgggcct gatcggggtt gacgtcacct
     1921 gcttcaaccc ggaaaaggat cccgacgaga gatgcgggac ggagctggcc gaactgctgc
     1981 gcggaaccct gaatggatga cccggcggct cgcgccatga gtgccccata agatcccgcc
     2041 atgctcgaag aaggcgacaa agctcccgat ttcacacttg aggaccagct cggggagaag
     2101 gtaaagctct ccgatcaccg caaggaactg gtcgtcctct acttctatcc gaaggccagt
     2161 accagcgggt gtaccaccca ggcctgctcg atccgcgaca acagtgccga ttacgcgaag
     2221 gcgggcgcga cggtgatcgg gatctcgccg gacgaggtca aggacatctc aaggttcgcg
     2281 accaagaacg acctcgagtt caccttggtc gccgacaagg accacaaggt cgcggagaaa
     2341 tatggcacct gggtcgaaaa gtcgatgtac gggaacaagt acatgggcgt gcagcgggca
     2401 accttcatca tccggggcgg aaagatcctg cgggtcttcc ccaaggtcca gccgaagaag
     2461 cacgatggcc tcgtcctggg ggcactggcc gagatcaacg aggagaaccg agatgtctaa
     2521 ctgggacaag ggctcatacg aggtcactgc cgaacagatc gcgcccgcca ctcccgcggt
     2581 cgccgatgcc gtcgaaccgg tcaccggcaa gctcgccctc gacctcgcct gcggcaccgg
     2641 aaacgaagcg ctcgagctgg cccgccgcgg cgccagggtg accggatatg acggtgcccc
     2701 gcgcctgctc caggtggcgg gcgagagagc cgcagccgag ggactcgagc tggaacgggt
     2761 tcagggcgac ctcgcggagc ttcccttcga cgacgacagc ttcgagatcg tcacctctgt
     2821 cttcgggctc atcttcaccg gctcgccgca ggattccgcc gccgagatcg gccgggtcct
     2881 tcggccccag ggccggatcg ccttcacttc atgggtcgag gaaggtctct tccagtacat
     2941 gcaggacatg gcgaaggcgg cagttgcctc ccactttggc caggcgccgc cggaaggcgc
     3001 cgacgccccg ttcgcgtggg gcgacgaggt cgccgtgcgt gaactgttct ccgaaagcgg
     3061 cctcatgctg caggtcgaaa ccagggaact cgtgatcgaa gaggaatcgg ccgcagcgct
     3121 gaacgaccgc tggttcgacc ttcacccgat ctggatcacc atgcaggaag tgatcggcga
     3181 cgaggcctac gaagagatgc gccgcgaggc gttgccggtg gtcgaatccc acaacgaagt
     3241 cagcgacgga agcttccgca acacgctgcg ttacctcctc ttcgagggca gccccgtctg
     3301 aaaaaactcc cgcgagacga gacgttgcgt ctcgcctgga tgtgacgttt tgtgaacctg
     3361 cggcgagacg agacgttgcg tctcgtctgg atttgacgtt ttgtggtcct tcggcgagac
     3421 gggacgttgc gtctcgtctg gatttgacgt tttgtggtcc tgcggcgaga cgggacgttg
     3481 cgtctcgcct gggtttttcc ggctaatgcg cggtcgagtg gccggtgtcg ccgctgagga
     3541 tcacgaaggc gaggaaggtc aggtagagca gaccccagat cgcgatcgaa ggcttgttga
     3601 actggccgtt ccggcggagt gaccagtagg cgacgacacc gccggcgagg ccgaggagca
     3661 tcgagaggac cgcgtacttg tccagcgacc agtcgatgaa gatcatgccg atcgcgacgg
     3721 ggatggtcga ctgaaagacc attgctccgg tgatgttgcc gagggcgagg gcatccttgc
     3781 cgtcgcggac ccagaagaac gagttcgcct tctccggcag ctcggtcgcc agcggcgcga
     3841 ggatcagcga gaggatgatc gccgaaacgc cgagatgctc ggccacgctc agcagttcgt
     3901 ggacgaagag atgggcaccg ccgaccattg aggcgagacc gaccagcagc tggatgatca
     3961 tgatcgagtt gggcggcccg ccggtgcggt cggtccggcg atccatgatc aggggatcca
     4021 tctcgtggct ctcggcaacg tcaccgctgt tgcggatcgt ctgcaccacg tagtagacgt
     4081 aagccaggag gcagatcact ccgccgccga tctgcaccga ctgtggaacg tcaaccaccc
     4141 cgaccaccag ggccagggtg aagaagatca tgaagaagac caggtcccgg tcgagggttt
     4201 cgacgtggac gtcgaggtgc ttgccctgcg gccggcgttt gatgtaggcg agcgccgaga
     4261 tgccgaccag ggccatcgcg atcgtcgcca gcaggaacgg cgccccgacg atcgccccga
     4321 ccgcaacctc gtcggagcca ctgaccccgc cgatgatcgc gacgatcggg atcagggttt
     4381 ccggcatcgc ggtgcccact gccgccagga tcgaaccgac cgcgccctca cccaggttga
     4441 gcttgtgacc gagccactcg atcgcgttgg tgaagagcag cgcgccggtc aggatgatcg
     4501 cgaagctgaa aagcaggagc aggtattcca tgggtaaggg ggaggtcctc taggtctggt
     4561 ttgcgccgga tggtcgcggc gtcaggctga accgtatccg ggcaggcgga agtgcattgt
     4621 ccgggtcgtc aagggccagg gtcagatcga gccggcggat gtcgccatcc tgctggccgg
     4681 cgtacaggtc gaaatcggct gaaacgagtg aatcctcaag gctggatccg gttttgaccc
     4741 cggcgatctg gcccacctcc ccgcccccgg ccgcttccag tgccctggcc aggccggcca
     4801 cgtcgagttc accggagatg tggtcggtcc cgaccccgga aacctcttcg gtcccgtcgt
     4861 accgaaggtc gccgaccagt ccgcgcagcc ccgaatcgga gcccagggcg ctgcggatgt
     4921 cagacatcac ctgcgggtcg gccgccacct tccgctcgtc cagaacccgg tcctcgtagc
     4981 caagtgcctc gaccgaaacg atgcccccgg tgggaccgcc cgatccgttg ccgaagctcg
     5041 aaagatgctc cctggtcagg gcccggtcca gaatcgtcgc cgggtccggg tcactgctgc
     5101 cgcaggcctg catcaccagc gagaacgcca gcacgagcac cagaacaagg gggatccgcg
     5161 tccgttccaa gtcggggcag gctatacgct cgcccttgaa tgccaccccg caccatcctc
     5221 tacacaggca aaggaggcgt tggcaagacc agcgttgccg ccgccaccgc ccgtcgctgc
     5281 gcagccgaag gcagacgcac ggtgatcctc tcgaccgacc ccgcccacag cctctccgat
     5341 tccctcgggg tcgaactcgg ccctgatccg gtcgaggttt cgcccgggct ttggggccag
     5401 gaggtgctcg ccgaagccga gatgcgccgc aactgggacc gggtcgccgc ctggatgagc
     5461 gggctgctgc agagcaaggg ggtcgaccag gtgcgggccg aggagttgag cgtcccgccc
     5521 ggactcgacg agcttttctc gctgcttcag atcaagcgcc actgggacaa gtcggaattt
     5581 gacgtggtga tcgtcgattg cgctccgagc ggcgaaaccc tgcggttgct cggctacccg
     5641 gaagtcgcaa gctggtgggt gcgaaaggtc ttcccctgga accgcagcct gctgagcaac
     5701 gccgcgccga tcgccaaggc gctcgagatc ctgatgcccg agcccgagct gat
//
LOCUS       DAOQMF010000016         5637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_6345_length_5637_cov_3.466858, whole genome shotgun sequence.
ACCESSION   DAOQMF010000016 DAOQMF010000000
VERSION     DAOQMF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6345_length_5637_cov_3.466858"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5637)
                     /locus_tag="PLV77_00600"
     CDS             complement(<1..>5637)
                     /locus_tag="PLV77_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNC14379.1"
                     /translation="MSKTTSGSVLLGGNSNVTLTASNPNGQPTGYNLSFRDVLPPGIS
                     YVPGSSPSSTGEPQVIANAPAAGQTTLIWSNVADLTPNSTFTFTFQVTNSPAVFDIGD
                     TYQNTAGSYLNCDPRYVPKFNAQGQPTQSGGNATCSGLPQETSYTGSATASANTLIKA
                     IEIDKSEPSPEGQLLRGLHDHQTVYTLTVTNNEVDADDNVKVEDYLPAGLEFLGCGTQ
                     DNTTDAPTNPGSAEEYPGSGAINPGNAPAAPDCVVPDTVETVLNPSGLPAGVYTHVVW
                     NNLGNLGPSGQLRIQYVAAVPIRQNTMTWTNGVPPVTGTQGSNLDNNSGPETTHLQGL
                     TNHAKVTSDHQGPGGPTEVTDSEDHTVQAVDIRIVKSVTPAGLAVGQISTWSLAIATS
                     EYRYFEDVEVSDTLGDGYCPLGPVNYEHTPPPATAECNPTGNNPSVPYTSAVEQTNGT
                     WQITWDSSTAPALGTINPNSNLTISFPTKTREYFQQNYVNSTPVLSNDTGRNDVDIAG
                     AAWVICAPGAPSPCPNLSPDKIDTDFTDGTVLDDDSDATQVGSGPSINKQVSPTVNGN
                     CGSLNDYASGITPVASPGDTICWRLTMSFPNNLTTSNVFVGDYIPPGTTYVFNSTVAL
                     PSNTVGIFTGSPPQPDLFGTSGLRWALNDGGGEVQPGKVFDVVFATKVQRSTDSVDGD
                     IEGNLMKAVYTNSDGTSFPLRDEEDFERSQPEISLLKGVYKVNGQPPAGNPPNVDGAV
                     VDGGDDVTYRVDVTNDGSVNAINTQVWDILPPQVTCADVDAGSISNGGTCNAGQNRIE
                     WSGLTVPATGNLTLTYTVEIPDGLSGGDVLNNTAGVREFASSSGSGPYVQVPENNIDP
                     TLDPNANAPAAKDPSNVVIESSTTVKTRVTDINQPGNSPTTQATIGERINYTVTATIP
                     QGTRLYGPTALVDNVSSRMTVVPLSASATLDNGTGGGPVPLPTAGLTLTQTGNVVKVD
                     FPNPYSNPEHSGDDVIVLTFGAIVRDVYPTNYAQGNSTQYTMPNTGTLSWKDTNGTNR
                     SASGSVNTTIVEPRISIDKTVDDDGPVSPNDLLNYTIQVSNGSAARVSTANNTVLTDK
                     VPVGLTPANGGVPVADGGTVNPDGGIWNLATRTITWPTIPTINPGAITNITYQARVDN
                     PAVGSDLLINHAQAATTSMPGTPTVERDSSTAAPGYLVSDEVPVEVSSPAIVKTGLPA
                     QATIGQHVTVTVFASIAANLTNYDGTVEDDLPNGLIFDNYVSEECIEGCSGPVTISTL
                     GVVPNGTGQRIGWWIGDVPAAPDSRVVALTYRAHVADQYQVPPGGPVLDGDALVNTAT
                     LLYNLTDKVTVPPTSPPDPSTFDRSLDDDHTTTVVEPDLALDKSVSGDDDQDDSRTTQ
                     PGDSYTFSIKVTNNGTSPAYDLTVTDQPDPALTNIQVNHSPVFEVPVLPVNSNDPIQW
                     FIPGPIDPGESVTLTYTADLVPSAQLHNMQQVVNTAELPSYWGVSAGEREDPDNAGID
                     YREYEGNNDTVTMTVLVPTVTLDKTTGLPQFPDTGDAQTETPFPWRILITNPNTGSSL
                     LGVDLTDTLPPNWTYVPNSAQITGTGTLTPGGQVNPTITQNPTGDVLVWSNIANLVGV
                     QNAVVSFNAIPEPEAAIDPGLTNPHVNEAEAIGEDTSGATASGDGPYTDDDDATATLE
                     TPVTDIQIVKTADDPAPTAGTNTTWTLVVKNNGPKVSPDVEVSDVLPAGLTYVSATSS
                     QGTCAENPAGTMHCQIGQMNVDDEVTIHLTTAVGAGTVGQTLTNPADVDDPYIDDSNP
                     ANNHSEDDVIPTESADVGIGKSIDEPLFAGQTGSYKLTVTNHGPSVARGVTVTDTLPE
                     NLTYAGATTPVGSCSGSGQDFSCDLGDLDPGQTVVI"
BASE COUNT          833 a         1625 c         2031 g         1148 t
ORIGIN      
        1 cgatcaccac ggtctggccg gggtcgagat caccgagatc gcaggagaag tcctgtccgg
       61 aaccgctgca gctgccgacc ggggtcgtgg cgccggcata ggtcaggttc tccggcagcg
      121 tgtcggtgac ggtcactccg cgggcgacgc tggggccgtg gttggtgacg gtcagtttgt
      181 agctgccggt ctggccggcg aagagcggct cgtcgatcga cttcccgatc ccgacgtcgg
      241 ccgactcggt cgggatcacg tcgtcttctg aatggttgtt ggccgggttc gaatcgtcga
      301 tgtaggggtc gtcgacatcg gccgggttgg tcagggtctg gccgaccgtg ccggccccga
      361 ccgccgtggt caggtggatc gtcacttcgt catcgacgtt catctgcccg atctggcaat
      421 gcatggttcc cgccgggttt tccgcacagg tcccctggga ggaagtggcg ctcacgtagg
      481 tgagcccggc cggcaggacg tcacttacct cgacgtcagg cgagaccttg ggcccgttgt
      541 tcttcaccac gagggtccag gtcgtgttcg tgcccgccgt cggggccggg tcatcggcgg
      601 tcttgacgat ctggatgtcg gtgaccggag tttcgagggt cgccgtggcg tcatcgtcgt
      661 ccgtgtatgg cccgtctccg gaggcggtag cgccggaagt gtcttccccg attgcctcgg
      721 cctcgttcac atgcgggttg gtcagccccg gatcgatcgc cgcttccggc tccgggatcg
      781 cgttgaagga gacaacggcg ttctggactc cgaccagatt cgcgatgttc gaccagacca
      841 ggacgtcacc ggtcgggttc tgggtgatcg tcggattgac ctggccgccc ggggtcaggg
      901 tgccggtgcc ggtgatctgg gccgagttcg gcacataggt ccagttcggc ggcagggtgt
      961 cggtcagatc gacgccgagc aggctgctgc ccgtgttcgg attggtgatc aggatccgcc
     1021 acgggaaggg cgtctcggtc tgggcgtcac cggtgtcggg gaactgcggc agcccggtcg
     1081 tcttgtcgag ggtaacggtc gggaccagca ccgtcatggt cacggtgtcg ttgttgccct
     1141 cgtactcccg gtagtcgatg ccggcgttgt ccggatcctc gcgttccccg gccgaaacgc
     1201 cccaatagga aggcagctcg gcggtgttca ctacctgctg catgttgtgc agctgggcgg
     1261 acggcaccag atcggccgtg taggtaaggg tgaccgactc gccgggatcg atcgggccgg
     1321 gtatgaacca ctggatcgga tcattcgaat tgacgggaag gaccggcacc tcgaagaccg
     1381 gcgaatggtt gacctggatg ttggtcagcg ccgggtcagg ctggtcggtg accgtcaggt
     1441 cataggccgg ggaagtcccg ttgttggtga ccttgatcga gaacgtgtag ctgtcaccgg
     1501 gctgcgtggt ccgggaatcg tcttggtcat cgtcaccgga aaccgatttg tcgagtgcca
     1561 ggtcgggctc gaccacggtc gtggtgtgat cgtcatcgag gctccggtcg aaggtggagg
     1621 gatccggcgg agaggtcggc ggcaccgtga ccttgtcggt caggttgtag agcagggtcg
     1681 ccgtgttgac cagggcatcg ccgtccagca caggtccgcc gggcggcacc tggtactggt
     1741 cggcgacatg ggccctgtag gtgagggcca cgacccggct gtcgggcgcc gcgggcacgt
     1801 cgccaatcca ccagccgatc cgctggccgg tcccgttcgg caccacgccc agggtggaaa
     1861 tcgtgaccgg cccggaacat ccctcgatgc attcctcgct gacgtaattg tcgaagatca
     1921 gcccgttggg aaggtcatcc tccacggttc cgtcgtagtt ggtcaggttg gcggcgatcg
     1981 aagcgaagac ggtcacggtc acgtgttggc cgatcgtcgc ctgggcggga aggccggtct
     2041 tgacgatcgc cggggaactc acttcgaccg ggacttcgtc cgaaaccagg tagccgggtg
     2101 ccgcggtcga agaatcacgc tccacggtcg gggtgccggg catcgaggtg gttgccgcct
     2161 gggcgtggtt gatcaggagg tcggaaccga cggccgggtt gtcgaccctg gcctggtagg
     2221 tgatgttcgt gatcgcaccg ggattgatcg tcgggatcgt cggccaggtg atcgtgcgcg
     2281 tcgcaaggtt ccagatgccg ccatccggat tcacggtgcc accatcggcg accggcactc
     2341 cgccgttggc cggcgtcaga ccgaccggca ccttgtcggt cagcaccgtg ttgttggcgg
     2401 tcgagaccct agccgccgag ccgttcgaaa cctggatcgt gtagttgagc agatcgttcg
     2461 gactcaccgg tccgtcatcg tcgactgtct tgtcgatcga gatccgtggc tcgacgatcg
     2521 tggtgttgac cgacccggag gcgctgcggt tggtgccgtt ggtgtccttc cagctcaggg
     2581 ttccggtatt cggcatcgtg tactgggtcg agttgccctg ggcgtagttg gtcggataga
     2641 cgtcccgcac gatcgccccg aaagtaagga cgatcacgtc atcgccgctg tgttccgggt
     2701 tcgaatacgg gttggggaaa tcgaccttta cgacgttgcc ggtctgggtc aaagtgagcc
     2761 cggccgtcgg cagtggcacc ggtccgccgc ccgtgccgtt gtccagggtg gcgctggcag
     2821 aaagcggaac cacggtcatc cgggagctca cgttgtcaac cagggcggtc ggcccgtaga
     2881 ggcgtgtgcc ctgcggaatc gtggcggtca ccgtgtagtt gattcgctca ccgatcgtgg
     2941 cctgggtggt cggactgttg cccggctggt tgatgtcggt gaccctggtc ttgacggtgg
     3001 ttgaagattc gatcaccacg tttgagggat ccttggccgc gggcgcgttg gcgttcggat
     3061 ccagggtcgg gtcgatgttg ttttccggca cctggacata ggggccggaa ccactggaag
     3121 aagcgaactc gcgaacaccg gcagtgttgt tgagcacgtc cccgccgctc aagccatcag
     3181 gtatttcaac ggtgtaagtc agggtcaggt tgccggtggc gggaacggtc agccccgacc
     3241 attcgatccg gttctgcccc gcgttgcagg taccgccgtt cgagatcgaa ccggcgtcga
     3301 cgtcggcaca ggtgacctgc ggcggcagga tgtcccagac ctgcgtgttg atcgcgttca
     3361 ccgagccgtc gttggtcacg tcgacgcgat aggtcacgtc atcgcctccg tcgacgacgg
     3421 ccccgtcaac gtttggaggg ttgcccgccg gcggctgtcc gttgaccttg tagaccccct
     3481 tgaggagact gatctcgggc tgggaccttt cgaaatcctc ttcgtcccga agcgggaacg
     3541 aggttccgtc ggagttggtg tagaccgcct tcatcaggtt gccttcgatg tcaccgtcca
     3601 cggaatcggt gctgcgctgg accttcgttg cgaagaccac gtcgaagacc ttgccgggct
     3661 ggacttcacc cccgccgtcg ttgagggccc agcggaggcc ggaggtacca aacaggtcgg
     3721 gctgcggggg cgagccggtg aagatgccga ccgtgttgga gggcagcgcc acggtgctgt
     3781 tgaagacata ggtcgtgccg ggcgggatgt aatcgccgac gaacacgttg ctggtcgtca
     3841 ggttgttcgg gaacgacatg gtcaggcgcc agcagatggt gtcacccggg ctggcgaccg
     3901 gggtgatgcc gctcgcgtag tcgttcaggc tgccgcagtt tccgttgacg gtcgggctta
     3961 cctgcttgtt gatgctgggc ccggacccga cctgggtcgc gtccgaatcg tcatcgagaa
     4021 cggtgccatc ggtgaagtcg gtgtcgatct tgtccgggct caggttcggg cagggcgagg
     4081 gggcaccggg ggcacagatg acccaggcgg ccccggctat gtctacgtcg ttccggccgg
     4141 tgtcgttgga gaggaccggg gttgaattca cgtagttctg ctggaagtac tcgcgggtct
     4201 tcgtcgggaa gctgatcgtc aggttgctgt tcggattgat cgtcccgagt gcgggagcgg
     4261 tgctcgaatc ccaggtgatc tgccaggtgc cgttggtctg ctcgaccgcc gaggtgtagg
     4321 gaaccgacgg gttgttgccg gtcgggttgc actcggcggt tgccggcggc ggggtgtgct
     4381 cgtagttgac cgggcccagc gggcagtagc cgtcgccgag cgtgtcgctg acctcgacat
     4441 cctcgaagta gcggtactcg ctggtcgcga tcgccagcga ccaggtgctg atctggccga
     4501 ccgccagacc ggccggcgtg accgacttga cgatcctgat gtcaaccgcc tgaacggtgt
     4561 ggtcctcgga gtcagtgacc tcggtcgggc cgcccgggcc ctgatggtcg gaggtcacct
     4621 tcgcgtggtt ggtcagcccc tggaggtggg tggtttcggg acccgaattg ttgtcgaggt
     4681 tggaaccctg cgtcccggtt accggaggaa caccgttggt ccaggtcatg gtgttctgcc
     4741 ggatcggcac tgccgccacg tactggatcc ggagctggcc ggaaggcccc aggttgccga
     4801 ggttgttcca gaccacatgc gtgtagaccc cggccggcaa gcctgagggg ttgagcaccg
     4861 tttcgacggt gtcggggacc acgcaatccg gcgcggcagg agcgttgccc gggttgatcg
     4921 cgcccgagcc ggggtactcc tccgcgctgc ccgggttggt cggcgcgtcg gtcgtgttgt
     4981 cctgggtgcc acaaccgagg aactcgaggc cggccggcag gtaatcctcg accttcacgt
     5041 tgtcgtcggc atcgacctcg ttgttggtga cggtcagcgt gtagacggtc tggtggtcgt
     5101 gcagaccccg gagcagctgc ccctccgggg agggctcgga cttgtcgatc tcgatcgcct
     5161 tgatcagcgt gttggccgaa gcggtcgccg agcccgtgta cgaagtctcc tgaggcaggc
     5221 ctgaacaggt ggcgtttccg ccgctctggg tcggctggcc ctgggcgttg aacttcggca
     5281 cgtaacgcgg gtcacagttc agatagctgc cggccgtgtt ctggtaggtg tcgccgatgt
     5341 cgaagaccgc cgggctgttg gtgacctgaa aagtgaaggt gaaggtcgaa ttgggggtga
     5401 gatcggcgac gtttgaccag atcagcgtgg tctgcccggc agcgggggcg ttcgcgatca
     5461 cctgtggctc gccggtgctg gacggtgagg aaccggggac gtaactgatc cccggcggga
     5521 gaacgtcccg aaagctgagg ttgtagccgg tcggctggcc gttcgggttc gaggcggtaa
     5581 gggtgacgtt cgagttaccc ccgagcagaa cggatccgct ggtggtcttg gacatcg
//
LOCUS       DAOQMF010000017         5624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_6372_length_5624_cov_3.377447, whole genome shotgun sequence.
ACCESSION   DAOQMF010000017 DAOQMF010000000
VERSION     DAOQMF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6372_length_5624_cov_3.377447"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLV77_00605"
     CDS             <1..682
                     /locus_tag="PLV77_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC14380.1"
                     /translation="GTRSLLVGGESHRPGTEDSAVSVARLRQFMRSRFEVRSVDCAWG
                     AHDMMSFDRLPIVGRLLPFDSRILTATGFSKWGLAAGAGSSELLADQLLGEDNPVAAT
                     FDPVRLRLSSVPTLVRHNLESGAMLLADRINRRRPRRRLQPGEGMVVGDGVGQKAVSC
                     DRDGVYRELSARCTHLGCIVAWNQAGQSWDCPCHGSRFSPDGSVIEGPAVAPLERSQV
                     ITPPAGDS"
     gene            complement(679..2508)
                     /locus_tag="PLV77_00610"
     CDS             complement(679..2508)
                     /locus_tag="PLV77_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNC14381.1"
                     /translation="MGHYKSNVRDIEFNLFEMFDTDGVLKTGAFGDLDSDTVRMMLEE
                     ASKLAEGPVAAAFESSDRTPPSFDADTHDVSLPADFKASVNAWTEADWEKIGLIEEVG
                     GIPAPATVSWAINEFMCGALPAGFFYLAGPAFASLLYKNGNEEQKHWAQMAVEKNWGA
                     TMVLTEPEAGSDVGAGRTKAIDQGDGTWHIEGEKRFITSGDSDDLFENIFHYTLARPE
                     GAGPGTKGLSLFVVPKWHFDSETGELGARNGVNVTSIEHKMGIKASATCDMAFGDGDN
                     PAVGYLVGDVHQGIRQMFDVIEFARMMVGTKAIATLSTGYLNALEYAKERVQGADLTQ
                     IMDKTAPRVTVIAHPDVRRSLMTQKTYAEGLRALYLFTALHHDPVAAEVSQGVDAEFA
                     AKINDFLLPIVKGVGSERAYEVLTESLQTYGGSGFLQDYPIEQYIRDAKIDSLYEGTT
                     AIQAQDFFFRKIVRDEAKSLNHLLEKMDATAAGSGAGLELERERLTAAIASMREIVGS
                     MMGYLMGSQEDPKSIYKVGLGSVQFLKAFGDLLIGWLLISQAEIAAAALENGAAGRDR
                     DFYEGKITGARFFARNMLPNLEATKLAIAQIDNDVMELDEAAF"
     gene            2679..4322
                     /locus_tag="PLV77_00615"
     CDS             2679..4322
                     /locus_tag="PLV77_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14382.1"
                     /translation="MNSEEIREPGARRGWLALAAMAAMLLMAAPAARAAECPDVINAS
                     AFASKARLHQMTAKFNSFGPRILGSPAHNQAIAWLEGQARSIGLRVHSTYFHPYTWLP
                     RTRMKKGPGLDIGAAGAIRVRKQDGSILEVADAGAVHWSKPTSSKGEGGPLVYLPADQ
                     QITAENAAGKVVIRDFRIGSIPFGVVGSLLGLYQTPDLEGTTDYARPYLSPNIHADQL
                     DASAAGANGVIFVFDLPASQIRGYYDPHVGMIYRQPAVFVGRSQGERLKELAGRGTSA
                     RVVVNANLARRKTRNLIARLPGQSNEKMVMVANTDGTSWVQENGVIGMLAFARYYAKL
                     PLRCRPRTLELVFSSAHDSYRNDGMSPRHYPLDKKRIAFGFAIEHLGTREVIPTGEGT
                     SRHLAFTGVADPALFGAGDSDALREAAVEATKRRNLPRTAVLKGLSVPNPDQAPSICS
                     MGGLGNSFQPELVPTLAMISGPWSLYDPVFGEKAIDFGQMRKQVLAAGDTVLALDGLP
                     RAEIAGEYPALQAELDAGTKTPCPETDVFPQRAPGPGGV"
     gene            4373..>5624
                     /locus_tag="PLV77_00620"
     CDS             4373..>5624
                     /locus_tag="PLV77_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNC14383.1"
                     /translation="MNQDSTTRIEPYVWKVAGVVILGMIMSILDTTIVNVALDTLAKD
                     LHTTISEIQWVATGYLLSLAAVIPLTGWAARKFGAKNVYIWSIVLFTLGSALCGIAAS
                     STSLIAFRVIQGIGGGMIMPIAQMIMAEVAGPKRMGKVMSVTVMPAMLAPILGPTIGG
                     LILQNFHWSWIFFVNVPVGIVAVLLAVRMLPHSKLGEPGKLDWAGLVLMVLGIPSLVF
                     GISEVGTTGTFNSPEVIWPVIAGLLLIGAFIRHAYRAERPLLDVRLYANRVFGAASLT
                     TFALGAALFGAMILMPLYYQQIRGESVIATGLLLAPQGLGMLMVGPFAPRLTERFGGG
                     KVATFGVAVLSLGSLPFVFIGVDTSIAFLSLMLLVRGIGIGLAFMPTMTVAYASLRKD
                     QLSDATPQMNVIQRVGGAIGTAVLA"
BASE COUNT          917 a         1808 c         1841 g         1058 t
ORIGIN      
        1 aggtacgcgc tcgttgctgg tcggcggaga gagccatcgt ccgggcaccg aggacagtgc
       61 cgtgagcgtt gcccgcctgc gccagttcat gaggtcaagg ttcgaggtcc gctccgtgga
      121 ctgtgcctgg ggcgcccacg acatgatgag cttcgaccgc ctgccgatcg tcgggcggct
      181 gctgcccttc gactcgagaa tcctcaccgc gaccggattc tccaaatggg gtctcgccgc
      241 gggcgcaggc agttccgagc tgctcgcaga tcaattgctg ggggaagaca acccggtcgc
      301 ggcaaccttc gaccccgtcc gtttacggct gagttcggtg ccgaccctgg tccggcacaa
      361 tctcgaatcc ggagccatgc tgctggccga ccggatcaac cgccgccggc cccggcgccg
      421 cctgcagccg ggcgagggca tggtggttgg cgacggcgtc ggccagaaag cggtgagttg
      481 cgaccgcgac ggcgtctacc gggaactctc ggcccgctgc acccatctcg gctgcatcgt
      541 ggcctggaac caggcggggc agtcctggga ctgcccctgc cacggttcgc gcttttcacc
      601 ggacgggagc gtgatcgagg gtccggccgt tgctccgctc gagcgaagcc aggtcatcac
      661 gcccccggcc ggggactcct agaaggcggc ttcgtcgagc tccatgacgt cgttgtcgat
      721 ctgggcgatc gccagcttgg tcgcttccag gttcggcagc atgttgcggg cgaagaacct
      781 ggcgccggtg atcttgcctt cgtagaagtc cctgtcgcgc ccggcggcac cgttctcgag
      841 ggcggctgcg gcgatctcgg cctggctgat cagcagccag ccgatcagca ggtcgccgaa
      901 ggccttgagg aactggaccg aaccgagacc gaccttgtag atgctcttcg gatcttcctg
      961 tgagcccatc aggtaaccca tcatcgaacc cacgatctcg cgcatgctgg cgatcgcggc
     1021 agtcaggcgt tccctttcga gctcgagccc ggcgccggaa ccggcggcgg tggcatccat
     1081 cttctcgagc aggtggttga gcgacttcgc ctcgtcacgg acgatcttgc ggaagaagaa
     1141 gtcctgcgcc tggatcgctg tagtgccctc gtagaggctg tcgatcttgg cgtcacggat
     1201 gtactgctcg atcgggtagt cctgcaggaa gcccgaaccg ccgtaggtct ggagcgattc
     1261 ggtcagcact tcgtaggcac gctctgaacc gacgcccttg acgatcggca gcaggaagtc
     1321 gttgatcttc gccgcgaact ctgcgtcaac cccctgcgag acctcggccg cgaccgggtc
     1381 gtggtggaga gcggtgaaga ggtaaagcgc ccggaggccc tcggcgtagg tcttctgggt
     1441 catcagtgac cggcggacgt cgggatgggc gatgacggtt acccgcgggg cggtcttgtc
     1501 catgatctgg gtcaggtcgg ccccctggac ccgttccttc gcgtactcga gcgcgttgag
     1561 gtaaccggtc gaaagggtgg cgatcgcctt ggtgccgacc atcatgcggg cgaactcgat
     1621 cacgtcgaac atctggcgaa tgccctggtg gacgtcaccg accaggtagc cgacggccgg
     1681 gttgtcgccg tccccgaagg ccatgtcgca ggtggccgaa gccttgatcc ccatcttgtg
     1741 ttcgatcgag gtgacgttga cgccgttgcg ggcgccgagc tcaccggttt ccgaatcgaa
     1801 atgccacttc ggcacgacga agaggctgag gcccttggtg ccggggccgg ctccttcggg
     1861 gcgggccagt gtgtagtgga agatgttctc gaagagatcg tccgagtctc cggaggtgat
     1921 gaagcgcttc tcgccctcga tgtgccaggt gccgtcaccc tggtcgatcg ccttggtgcg
     1981 gccggcgccg acgtcggagc cggcttcggg ctcggtcagg accatggtcg cgccccagtt
     2041 cttctcgacc gccatctgtg cccagtgctt ctgttcctcg ttgccgttct tgtagagcag
     2101 gctggcgaag gcggggccgg cgaggtagaa gaagccggcc ggcagggcgc cgcacatgaa
     2161 ctcgttgatc gcccaggaga ccgtcgccgg ggcggggatg ccgccgactt cttcgatcag
     2221 gccgatcttc tcccagtcgg cttcggtcca ggcgttgacc gacgccttga agtctgccgg
     2281 caggcttacg tcgtgcgtat cggcgtcaaa gctcggcgga gtccggtcac ttgattcgaa
     2341 agcggcggcg acgggtccct cggccagctt cgaggcttcc tccagcatca tcctgaccgt
     2401 gtccgaatcg agatcgccga atgcgccggt cttcaacact ccatcggtgt cgaacatctc
     2461 gaagaggttg aactcgatgt cccggacgtt gctcttgtaa tgacccactt tttctccgtt
     2521 cgaagccttt gacagttcct gcgaccggaa agcattccat cattcggtgt caaatgcgtg
     2581 gctgtccggg agcgctaggc ttctcgcgta gttccagcgg gaccgcaacc atcgctggtc
     2641 ggccgttttc gggaggggat tcaagcggga agatgaggat gaattcggag gaaatcaggg
     2701 aaccaggcgc caggcgaggt tggctcgcgc ttgccgcgat ggccgcgatg ctgctgatgg
     2761 cagctccggc ggcacgggcc gccgaatgcc cggacgtgat caacgcctcc gcttttgcca
     2821 gcaaggcgcg ccttcaccag atgacggcga agttcaacag cttcggtccg cgcattctcg
     2881 gcagcccggc ccacaaccag gcgatcgcat ggctggaagg gcaggcacgg tcgatcgggt
     2941 tgagagtgca ctcgacctac ttccatccct acacctggct tccccggacc cggatgaaga
     3001 agggccccgg gctcgacatc ggggccgccg gcgcgatcag ggtcaggaag caggacgggt
     3061 caatcctcga ggtggccgac gccggtgctg tgcattggtc gaagccaacc tcgagcaagg
     3121 gtgagggcgg gccgctcgtc taccttcctg ccgatcagca gatcaccgcc gaaaacgcgg
     3181 ccgggaaggt cgtgatcagg gatttccgga tcggatcgat tccgttcggg gtcgtcggtt
     3241 cgctgctcgg gctctaccag acgcccgacc ttgaggggac gaccgactat gcgcgaccgt
     3301 acctgagccc gaacatccac gccgatcagc tggatgcgag cgcggccggc gcaaacgggg
     3361 tgatcttcgt ctttgacctg ccggccagcc agatacgcgg ctactacgac ccgcatgtcg
     3421 gcatgatcta ccggcaaccg gcggtctttg tcggtcgatc ccagggtgag cgactcaagg
     3481 aactcgctgg ccggggtacc tcggctcggg tcgtggtgaa cgcgaacctc gctcgccgca
     3541 agacgcgcaa cctgatcgcc aggcttccgg gccaaagcaa cgagaagatg gtcatggtcg
     3601 caaacaccga tggcacctcc tgggtccagg agaacggcgt gatcggcatg ctcgccttcg
     3661 cccgctacta cgcgaagttg ccgctgcgct gtcggccccg caccctggag ctggtcttct
     3721 cctccgccca tgactcttac cgcaatgacg ggatgagccc ccgccattac ccgctggaca
     3781 agaagcggat cgcgttcggt ttcgcgatcg agcatctcgg tacccgcgag gtgataccga
     3841 ccggagaggg aacgagccgc catctcgcct tcaccggggt ggccgacccg gcactctttg
     3901 gcgccggtga cagtgacgcg ctgcgggaag ccgcggtcga ggccaccaag cgaaggaacc
     3961 ttccccgcac ggcggtgctc aaggggctga gcgtgcccaa ccccgaccag gccccgtcga
     4021 tctgctcgat gggagggctc ggcaactcct tccagcctga actggttccg accctggcga
     4081 tgatctctgg accctggtcc ctgtacgacc cggtgttcgg cgagaaggcg attgatttcg
     4141 gccagatgcg caagcaggtg ctggcggcgg gggacacggt cctggctctc gacgggctgc
     4201 cgcgcgccga gatcgccggc gagtatccgg ccctgcaggc ggagctggat gccggcacca
     4261 agacgccctg cccggaaacc gatgtcttcc cgcagcgggc accggggccc gggggagtgt
     4321 gacggattca cacttgacgg aaagtgatta ggagcctaat attaggttcc taatgaatca
     4381 ggattccact acccgcatcg agccgtacgt ctggaaggtc gcaggggtgg tgatcctcgg
     4441 catgatcatg tcgattctcg acacgacgat cgtcaacgtc gcgctcgaca ccctcgccaa
     4501 agacctgcac acgacgatca gcgagatcca gtgggtggcg accggctacc tgctttcgct
     4561 cgcggcggtg atccccctga ccggctgggc ggcgcggaaa ttcggggcaa agaacgtcta
     4621 catctggtcg atcgtcctgt tcacgctcgg ctcggccctt tgcggcatcg ctgcatcgag
     4681 cacttcgctg atcgccttcc gggtgatcca ggggatcggt ggagggatga tcatgccgat
     4741 cgcccagatg atcatggccg aggtcgccgg accgaaaagg atgggcaagg tcatgagcgt
     4801 caccgtgatg cccgccatgc tcgccccgat tctgggcccg acgatcggcg gtctcatcct
     4861 gcagaacttt cactggtcct ggatcttctt cgtcaacgtt ccggtcggca tcgtcgccgt
     4921 gctgctcgcc gtgaggatgc tgccccattc gaagctcggc gagcccggca aactcgactg
     4981 ggccggactg gtcctcatgg tgctcggcat tccgtccctg gtcttcggca tctccgaagt
     5041 cggcaccacc ggaactttca actcgccgga agtgatctgg ccggtcatcg ccggactgct
     5101 cctgatcggc gccttcatcc ggcacgcata ccgcgccgag cggccgctgc ttgacgtccg
     5161 gctctatgcg aaccgggtct tcggtgccgc ctcgctaacc accttcgcgc tgggtgccgc
     5221 gctctttgga gcgatgatcc tgatgccgct ctactaccag cagatccgcg gcgaaagcgt
     5281 gatcgcgacc ggcctcctgc tggccccgca gggcctgggc atgctgatgg tcgggccctt
     5341 cgctccccgc ctcacggagc gcttcggcgg cggcaaggtc gcgaccttcg gagtcgccgt
     5401 gctcagcctc ggatcgcttc ccttcgtctt catcggggtt gacacttcga tcgccttcct
     5461 ctcgttgatg ctgctggtcc ggggcatcgg catcggtctc gccttcatgc cgacgatgac
     5521 cgtcgcctac gcatcgctga ggaaggacca gctttccgat gccacgccgc agatgaacgt
     5581 gatccagcgg gtgggtggcg cgatcggcac ggcggtactg gcgg
//
LOCUS       DAOQMF010000018         5605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_6414_length_5605_cov_3.545405, whole genome shotgun sequence.
ACCESSION   DAOQMF010000018 DAOQMF010000000
VERSION     DAOQMF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_5605_cov_3.545405"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLV77_00625"
     CDS             complement(<1..793)
                     /locus_tag="PLV77_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14384.1"
                     /translation="MPVLVNGLTDAVSISAGDDHTCALRASGQVLCWGADSYGAIGNG
                     PDAGSTTVPTPVVGLNDAVAIAAGDFHTCAVRASGQVVCWGLDMSGQLGDGTGLPNSK
                     PSPSAVQGIGDAVAVTAGEKYSCAARASGTTMCWGNGSGGKLGNYTLGTWDYGYPNSY
                     VPAVARNVGGATAVTAGADQSCAIQNGGQAVCWGSAPLGDGSNNDSSVAVRVQGLADA
                     KSIDSGTYGTCAVRDSGQAVCWGISPLPQLGNGTITPTPAQRIPSP"
     gene            complement(1272..>5605)
                     /locus_tag="PLV77_00630"
     CDS             complement(1272..>5605)
                     /locus_tag="PLV77_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HNC14385.1"
                     /translation="IYTPQNNIDPSNPRTPNIARVDDDSNVKTPDVGLVKARTTSITE
                     TGNSATSQATIGELINYTVTATLPNGTTFGTNAKITDTPNSATTQPLTGPATATLNGS
                     PLPSGWSISTVGQTITVNIPDGYVVPPGSDHTVVISFQTRVVDVASNVRGQSRTNQAN
                     ISWTDSTARSRNSNQVSTTIVEPLISQSKSNNKSTNAQPNDIVTYTLVTSNSSASNVS
                     IAHDTVIKDVVPVGVTLVDGGGNPLADGAVVPGTGGATWDAATRTITSAASPAININP
                     GGNVTWTYQARVDSPAIAGSVFTNTANAKTTSINGSDANERTASSSTNTGYSANSSST
                     VRIGGSSVTKSVDPAWVTIGTPMTYKATVTIPQGLEFFNLTARDILPDSIDFDGYTGW
                     SCISGCSGSNPAPTVQNYNPQVTSSVTNIGWDMGHLDPGTADRVIEFTYKAHVRDTHR
                     SGGAPVLAGENIVNSVRSMSNTSNKFTFNPNSIPAQSGFDHVSSEAKTTTPVREPKIV
                     LDKKVKIGGGSFVDGPVQSQPNDALTYQIVVTNNGTSPAYDVTVDDMPDADLTNVALA
                     TGAAYNTKMWSAGDRSMKWLIPGPIPVGGSVTLTYSANAVSSAQLDNNSQAINTADSD
                     YWGIPVAERTNPWTYRHYDSNQDTVVVNFEFPELTVTKTTGKPGFPDIADAPVEQSFP
                     WRIVVKNNATTAKAFDTVVRDVLPPFWTYDTGSTTITGATGGDPSVAPNASGDVLTWN
                     FNGQVIQPGASVVINFTATPGLGARANPPVQTNDSDAKTKDASGASGNHSGPYEDEDD
                     AKATLKFPIADLKIEKSAPAQVNVYDEFDYTLKITNKGPDTATQVTVSDPLPAGLVFV
                     SSADCNAVMTCSLGTMAANTSKTVTIRVKTTYLVGGTTVHNVATVTGHEWEPTPEDNT
                     DDADTFVVGLPDLKITKTAAPTNARPGDVVTYTLKAENVGNAVAHNVLITDPVPVGMT
                     FVSADSPCTESGGTVNCAISDLNPGQVKTYTVKATVDPWGDADPSADHLLDVQKVETQ
                     IDLNPGETKTVQAVCPSGYFASDGSVRIDHVDQGTGDWTAPEVLESRASSLDTWQGTV
                     HNSAAGRAQAKIFAVCISKQTVADGGHQHDLIVSAPITVTNLTPAGKNEATLQCGPGQ
                     IAIQSGFNSSVPADLVYSEPEGNGWKFILDSKVDGQATFSIRCMTRQVSVVDGHTHDL
                     QFQHIVTEFTFPAGQVSEAQLTCADGYKGIVADMDLDDGLVSLGNDPRPVTRAFKIYN
                     PTDHDLTGRLSLLCLGLRTAGEHVPPKVLQNTAYISTTSFEEVTTNNHSTATVTAEDT
                     DNYEPVEPTDPVKPTPNNPVAKTIVGKGVSYSATGVTFTLKCSGACGGFAKLSSLKKV
                     KVRGKSYRRGTVLAKKQYFIGKAGSKRIKLTLTRAGKGILKSGKVKKASLRINGGTSK
                     VVKIGRR"
BASE COUNT          942 a         1672 c         1842 g         1149 t
ORIGIN      
        1 ccgggctcgg aatacgctgg gcgggcgttg gtgtgattgt gccgttgcca agctgaggca
       61 agggactgat gccccagcag actgcctgac ccgaatcgcg taccgcgcag gttccgtaag
      121 ttccggaatc aatcgacttg gcgtcggcaa gaccctggac cctgacagcc acgctgctgt
      181 cgttgttgga tccatcaccg agaggcgctg atccccagca gaccgcctga ccgccattct
      241 ggatggcgca actttgatca gcaccggcag taacggcagt cgctccaccc acgttccggg
      301 ccaccgccgg gacatagctg ttgggatatc cgtagtccca ggtgcccaga gtgtaattgc
      361 caagcttccc gcccgaacca ttgccccagc acatggtcgt acctgaagcc cgggccgcgc
      421 agctgtattt ctctccggca gttaccgcga cggcatcacc aattccctga acagccgatg
      481 gcgacggctt gctgttggga agacccgtcc cgtcgccgag ttgtccggac atgtcaaggc
      541 cccagcagac cacctggcca gaggcccgga cggcacaagt gtggaagtcg ccggccgcta
      601 tggcgaccgc atcgttgagg ccgactaccg gggtcggcac ggtcgttgac ccagcgtcgg
      661 gcccattccc aatcgcgccg tagctgtcgg cgccccagca gagcacctgt ccggatgcgc
      721 gaagggcgca ggtgtgatcg tcaccggcgc tgattgaaac cgcgtcagtg agaccattga
      781 caaggacagg caccgtgcgc ctctcgtagg ttccatcacc aagctcgcct gttgaattgt
      841 ctccccagca agctgccctc ccggagttca acaccgcgca ggcgtggctc ggacctacag
      901 cgactgaagt tgcctctagc ggttgacttg cctggccggc cgatggcaca accagtccgc
      961 ctgccaacgc gaagatggcc gctacacaga acgcagccag aaaagtcaga gcgttcgagc
     1021 tacaggttgc tttggccatc gaactgctgc tgttctcccg gatattcatt tgacggctcc
     1081 ttgttctttg ggtgttcgac atgacactgt gaacaggacg gtcaagggtt cgcgggtctt
     1141 ccggattccc cgctcatgtg acctgactca cgcaagctgt ctggcgctca tcgacaggcg
     1201 ccggcatagc aacaaaaagc cccggtcttt cgaccggggc tcttttctcc ctggcagtga
     1261 gtggatcggg gctagcgccg cccgatcttc actaccttgc tggtccctcc attaatcctg
     1321 agtgaagcct tcttgacctt gcctgatttc aggatcccct taccggcccg ggtaagcgtc
     1381 agcttgatgc gcttactgcc tgccttgccg atgaagtact gcttcttcgc caggacggtg
     1441 ccccggcggt aactcttgcc cctgaccttg accttcttaa gggatgacag cttcgcgaag
     1501 ccgccacagg cacccgagca cttcagcgtg aaggtaacgc ccgtggccga gtagctgacg
     1561 cccttgccga cgatcgtctt cgcgaccggg ttgttcgggg tcggcttgac cgggtcggtc
     1621 ggttccaccg gctcgtagtt gtcggtgtcc tcggcggtca cagtcgcggt cgagtggttg
     1681 ttcgtcgtca cttcctcgaa agaagtcgtc gagatgtagg cggtgttctg aagcaccttc
     1741 ggcggcacat gctcacccgc ggtcctgaga ccgaggcaaa gcaggctgag ccttccggtc
     1801 aggtcatgat cggtcgggtt gtagatcttg aacgcacggg tcaccggacg cggatcgttg
     1861 cccaacgaaa ccaggccgtc atccaggtcc atgtcagcga cgatgccctt gtaaccgtcg
     1921 gcgcaggtga gctgggcctc agagacctga ccggccggga aggtgaactc cgtgacgatg
     1981 tgctggaact gaaggtcatg ggtatgacca tcgaccacac tgacctgacg ggtcatgcag
     2041 cgaatcgaga aagtcgcctg gccgtcaacc ttgctgtcaa ggatgaactt ccagccattg
     2101 ccctccggct cgctgtaaac cagatcggcc ggaacgctcg agttgaaacc tgactgaatc
     2161 gcgatctggc ccggaccgca ctgaagcgtc gcctcgttct tgccggccgg cgtcaggttg
     2221 gtcaccgtga tcggggccga gacgatcaga tcatgctggt ggccaccatc ggccacagtc
     2281 tgcttcgaaa tgcagaccgc gaagatcttc gcctgggcgc gaccagccgc cgagttgtgg
     2341 acggtgccct gccaggtatc cagcgaggaa gcacgcgact cgagaacttc gggggcggtc
     2401 cagtcaccgg tgccctgatc aacatggtcg atccgcaccg aaccgtcaga agcgaagtaa
     2461 ccggacgggc aaaccgcctg gaccgtcttc gtctcacccg gattcaggtc gatctgggtc
     2521 tccaccttct ggacatccag caggtgatcg gctgaaggat cggcgtcacc ccagggatcg
     2581 acagtggcct tgaccgtgta ggtcttgacc tgacccggat tgagatcact gatcgcgcaa
     2641 ttcaccgtgc cacccgactc ggtacagggc gaatcggcac tgacaaaggt catgccaacc
     2701 ggcaccggat cggtgatcaa cacattgtgg gcgaccgcgt taccgacgtt ctcggccttc
     2761 agggtgtaag tcacgacatc acccggacgc gcattcgtcg gcgccgcagt cttggtgatc
     2821 ttcaggtcgg gaagaccgac cacgaaggtg tcggcgtcat cggtgttgtc ttccggggtc
     2881 ggttcccatt cgtgaccggt cacggtcgcg acgttgtgaa cagtggtgcc accgaccagg
     2941 taggtggtct tgacccggat cgtgaccgtc ttcgaggtgt tcgccgccat ggtgcccagc
     3001 gagcaggtca tgactgcgtt gcagtcggcc gaagagacga acaccagacc ggccggcagc
     3061 ggatcgctga ccgtcacctg ggtcgcagtg tcgggaccct tgttggtgat cttcagggtg
     3121 tagtcgaact cgtcgtatac gttgacctgg gccggcgccg acttctcgat cttcaggtcg
     3181 gcgatcggga acttgagcgt cgccttggcg tcgtcctcgt cctcgtaagg accgctgtgg
     3241 ttgccactgg caccgctcgc gtccttggtc ttcgcgtccg agtcattggt ctggaccggc
     3301 gggttggcgc gggcgccgag accgggcgtg gcggtgaagt tgatgaccac cgatgcgccg
     3361 ggctggatga cctgaccgtt gaagttccag gtcaggacgt cgccggaggc gttcggagcg
     3421 accgacgggt cgccaccggt cgcgccggtg atcgtcgtcg atcctgtgtc gtaggtccag
     3481 aagggcggca gcacatcacg gacaaccgtg tcgaaggcct tcgcggtggt cgcgttgttc
     3541 ttgaccacga tgcgccaggg gaaggactgc tcgaccggag cgtcggcgat gtcggggaat
     3601 ccgggcttgc cagtggtctt ggtcacggtc agctcgggga actcgaagtt gaccacgacc
     3661 gtgtcctgat tgctgtcgta atggcggtag gtccacgggt ttgtccgctc ggcgaccggg
     3721 atgccccagt aatccgagtc ggccgtgttg attgcctgcg agttgttgtc gagctgggcc
     3781 gaggaaacgg cgttggcgct gtaggtcagg gtgacgctgc caccgaccgg gatcggaccc
     3841 gggatcagcc acttcatcga acggtcgccg gcgctccaca tcttggtgtt gtaggcggca
     3901 ccggtggcca gcgcgacgtt ggtcagatcg gcgtcgggca tgtcatccac ggtcacgtca
     3961 taggcggggc tggtgccgtt gttggtgacc acgatctggt aggtcagcgc gtcattcggc
     4021 tggctctgga ccgggccgtc aacgaagctg ccgccaccga tcttgacctt cttgtcgagg
     4081 acgatcttcg gctcacgaac cggagtggtc gtcttcgcct cggaggagac gtgatcgaag
     4141 ccgctctggg ccggaatcga gttcgggttg aaggtgaact tgttcgaggt gttcgacatc
     4201 gacctaaccg agttgacgat gttctcgccg gccagaaccg gggcaccgcc gctgcgatgc
     4261 gtgtcacgga catgcgcctt gtaggtgaac tcgatcaccc ggtcggccgt gcccggatca
     4321 aggtgaccca tgtcccagcc gatattggtc accgaggagg tgacctgcgg gttgtagttc
     4381 tggacagtcg gggccggatt ggagccggag caaccgctga tgcaggacca acccgtgtag
     4441 ccgtcaaagt cgatcgagtc cggcaggatg tcccgcgcgg tcaggttgaa gaactcgagc
     4501 ccctgcggga tcgtgacggt tgccttgtag gtcatcggcg tgccgatcgt gacccaggca
     4561 ggatcgaccg acttggtcac cgagctgccg ccgattctga cggtgctgct cgaattcgcc
     4621 gagtagcccg tgttggtgct gctcgatgcc gtgcgctcgt tcgcgtcgct gccgttgatc
     4681 gaggtggtct tcgcgttggc ggtgttggtg aagacgctgc cggcgatcgc cggggaatcg
     4741 accctggcct ggtaggtcca ggtcacgttt ccgcccgggt tgatgtttat ggcgggcgag
     4801 gcggccgagg tgatggtgcg ggttgccgca tcccaggttg cgccgccggt gccgggcaca
     4861 accgcaccgt cggccagcgg gttgccgccg ccgtcgacca gggtcacgcc gaccggaacc
     4921 acgtccttga tcaccgtgtc gtgggcgatg ctgacgttcg aggcgctgga gttcgaagtg
     4981 accagggtgt aggtgacgat gtcattcggc tgggcgttgg tcgacttgtt gttcgacttg
     5041 ctctgcgaga tcagcggctc gacgatggtg gtcgagacct ggttcgagtt gcggctgcgg
     5101 gcggtgctgt cagtccagga gatgttggcc tgattggtcc ggctctgacc gcggacattt
     5161 gacgccacat cgacgacccg ggtctggaaa ctgatcacga ccgtgtggtc ggatcccggc
     5221 ggaaccacgt atccgtcggg gatgttgacc gtgatggtct ggccgaccgt gctgatgctc
     5281 cagccggagg gcagcggtga accgttcagg gtcgcggtcg ccgggccggt cagcggctgg
     5341 gtcgtggccg agttcggggt atcggtgatc ttcgcgttcg taccgaaggt agtgccattc
     5401 ggcagggtcg cggtgaccgt gtagttgatc agctcgccaa tcgtcgcctg ggaagtggcg
     5461 ctgttgccgg tctcggtgat cgaggttgtc cgggccttga ccaacccgac atcgggggtc
     5521 ttgacgttgc tgtcgtcatc gacccgggcg atgttcgggg tgcgcggatt gctcggatca
     5581 atgttgttct gcggcgtgta gatgt
//
LOCUS       DAOQMF010000019         5454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_6738_length_5454_cov_3.743101, whole genome shotgun sequence.
ACCESSION   DAOQMF010000019 DAOQMF010000000
VERSION     DAOQMF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6738_length_5454_cov_3.743101"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLV77_00635"
     CDS             complement(<1..469)
                     /locus_tag="PLV77_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14386.1"
                     /translation="MFNTKNRVVFALLAIFTLSALLAGPAQAKRKHKPKKPNTVKVMS
                     RNLYLGADLGPAINSSDAADFISKNGQILRDVDTNNFPVRARGLAGEIRKVSPDLVGL
                     QEVALWRTGDASFTPSITGEYTASTVKVDFLADLLAQLNKGGVKRYRVVKVQNE"
     gene            complement(672..1079)
                     /locus_tag="PLV77_00640"
     CDS             complement(672..1079)
                     /locus_tag="PLV77_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC14387.1"
                     /translation="MNKKPFVIFGLLLILLAVVIPFWAFEKDGNTANNEGIPSESTPG
                     KQLFQTNCGNCHTLYAAGTDGDFGPNLDAKLAPAGPPADETAVKGIKTQVMAAIEEGA
                     DSPDVPGRMPAGIVTGTVADEIADFVAHYAGEG"
     gene            complement(1125..1901)
                     /gene="nth"
                     /locus_tag="PLV77_00645"
     CDS             complement(1125..1901)
                     /gene="nth"
                     /locus_tag="PLV77_00645"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNC14388.1"
                     /translation="MAAWRAKVAKKEWKRPTRARVRAIRDRLREMYGRPVNLPHRQPI
                     DELVRTILSQATSDANRDRAFAGLRDRFDDWEAVRDAPESEVEAAIRPGGLSRQKAPR
                     IQAVLREIEDLDLGWLEHADRALAMEFLTGLPGVGRKTAACVQVFTWNRPEIPVDVHV
                     FRIGGRLGLFRPNASFEEAHDELLALVDPADSYEFHMNLIRHGRTLCRPTPLCRECDL
                     RRMCPYGRGPGSERQPAGRTREGGPQSGVGTVGRGSGSEG"
     gene            complement(1905..2840)
                     /locus_tag="PLV77_00650"
     CDS             complement(1905..2840)
                     /locus_tag="PLV77_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC14389.1"
                     /translation="MERERPARPHSDQRPPIACLGEAIADLICERNLAKGEAPGPFVP
                     WPGGALANVAVAIARSGAPAALVGGVGGDGWGRWLADGLESEGVSTRWLATLDGADTP
                     VALIEFDSGNEPAFQVYGEHIGPTMAATGDFLEEAITGSRAVVIGSNTMVGEVERNVT
                     RRAVELAAAAGLPVLLDPNYRPNRWTSEEPARDFCRELSGSATVVKLNQGEAEMITGI
                     GEPLAAARDLLKLGPELVVVTNGEGEIVTAGAVEARHRPEPVETVSPLGAGDAFMGGL
                     AAGLAGLDWDLSRAAEVLPGAALAAAAVCGHWGAQ"
     gene            2910..3692
                     /locus_tag="PLV77_00655"
     CDS             2910..3692
                     /locus_tag="PLV77_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC14390.1"
                     /translation="MPALIVRIGEWLPRGWPDLFRQLLLYVLADTFYEMARGMADGRA
                     NVAFANGERVIDTERTLGMFFEPGVQSAFHGFTWLINIANTIYLNSQFTVALGFLIWM
                     YLFRNDYYYFFRNMLFVSMGIALVGYIGFPTAPPRMYPEYGFIDTVNAQSSVNHDSAL
                     AKLFINPFAAVPSMHCAVALMIGVTGFQVCRNWFAKTFWAGWPLVVAWVTVVTANHYW
                     IDAVLGWGVAGLSFLIASRVLAEIKPETWAWSREAERQRAEA"
     gene            3707..5317
                     /locus_tag="PLV77_00660"
     CDS             3707..5317
                     /locus_tag="PLV77_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14391.1"
                     /translation="MRLRSLTSPAAWGFLAAPLLVVVGLAVIYLIMAPHSAGHAAQTF
                     RTELFELSGPTVWNNYWFAGHYLPSYSLLSPPLGAWLGFRVMGSLAVIGTVILFTLIV
                     RREWGERAQAGAIWFAFAATISLFSGRLTFALGCLLAMAAVFASQRGLRIPALLLAGS
                     VGLASPVAALFLACLGFAHFLAERPDRRGLELAGVSFLVAAIVALLFPGGGDEPYVTS
                     SFIPAIGLTLVSAWMVPPGQRLVRTGMLVYAGALVASYVLSTPMGGNVNRLGALLLGP
                     VLLCVLAAEPLTPKLWRRGVLIILLPMIVWWQTGPVIRDLKLTDDDPAVTQAFYQPLA
                     DALEPRLVREPARVEVVPIASHWEAARAAPEFPLARGWERQTDRNFNPLFYADRLGPN
                     RYRQWLDRLAVGYVALPADTELDYAGEKEAALIRSGLPFLREIPVGGDWRLFKVIGAQ
                     PMVARPARLTELDTDGFAVAAPIAGSFEVRIRSTPYWKVKSGFGCVEPGRGDWTRVTL
                     DRPGSLKVAADFSPGARFGSDRDCRPDR"
BASE COUNT          775 a         1907 c         1806 g          966 t
ORIGIN      
        1 attcgttctg gaccttgacc acccggtagc gcttgactcc acccttgttg agctgcgcga
       61 gcaggtcggc gaggaagtcc accttgacgg tagaggccgt gtactcaccg gtgatcgagg
      121 gcgtgaagct ggcgtcgccg gtgcgccaga gagcgacttc ctgaaggccg acgaggtccg
      181 ggcttacctt cctgatttcg ccggcgaggc ccctggcccg gaccgggaag ttgttggtgt
      241 ccacgtccct caggatctgg ccgttcttgc tgatgaagtc ggcggcgtcg gacgagttga
      301 tcgccgggcc gaggtcggct cccagataca ggttgcggga catcaccttg acggtgttcg
      361 gcttcttcgg cttgtgcttg cgcttggcct gggccgggcc ggccagcagc gcggaaagcg
      421 tgaagatcgc cagcagggcg aagaccacgc ggttcttggt gttgaacatc aagttctctc
      481 cttttggtct ggggcgaggc gcggcaacgc gccttctgcg gttccccaac cgctgtcagg
      541 aggataaacc cggcaagggc ccgaacgatg cggagactga tcaatcggag ccggcccgac
      601 catcagcgaa tcaagcgggc cggcgggttc ccgttgtcat ccggcgaaga ccggatgacg
      661 ccttgttgtc cctaaccttc gccggcgtaa tgggcgacga agtcggcaat ctcgtcggcg
      721 accgtgccgg tgacgatgcc ggcgggcatg cggccgggaa cgtcggggct gtcggcgcct
      781 tcttcgatgg ccgccatgac ctgggtcttg attcccttga ctgcggtctc gtcggcgggc
      841 gggccggccg gagcgagctt ggcgtcgagg ttcgggccga agtcaccgtc ggtgccggcc
      901 gcgtagagcg tgtggcagtt gccgcagttg gtctggaaga gctgcttgcc cggcgtgctc
      961 tcggagggaa tcccctcgtt gttggcggtg ttgccgtcct tctcgaatgc ccagaacggg
     1021 atcaccactg ccagcaggat caggaggaga ccaaatatga cgaagggctt cttgttcatg
     1081 gacgggcggc gcaaaagtgg acggtgcgcc gaacgccggg aatattaccc ttcggacccc
     1141 gatccgcgtc ctaccgttcc gaccccgctc tgcgggccgc cttcccgggt gcgccccgcg
     1201 ggctgccgtt ccgaccccgg tccgcggccg tagggacaca tccggcgcag gtcgcactcg
     1261 cggcagagcg gcgtcggtcg gcaaagagtg cgcccgtgac ggatcaggtt catgtggaat
     1321 tcgtatgaat cggccgggtc gaccagcgcc agcagttcat cgtgggcttc ctcgaaggaa
     1381 gcgttcggcc ggaacagccc cagccttccg ccgatccgaa acacatgaac gtcgaccggg
     1441 atctccggcc ggttccaggt gaagacctga acgcaggccg ccgtcttgcg ccctaccccg
     1501 ggcagtccgg tcaggaactc catcgcaagg gcgcggtcgg cgtgttcgag ccagccgagg
     1561 tcgaggtcct cgatctccct gaggacggcc tggatccgcg gcgccttctg acggctgagg
     1621 cccccgggcc ggatcgccgc ctcgacctcg gactcggggg cgtcgcgaac cgcctcccag
     1681 tcatcaaagc ggtccctcag accggcaaag gcccggtcgc gattggcgtc actggtcgcc
     1741 tgggagagga tggtccggac cagctcgtcg atcggctggc ggtgcggcag gttgaccggg
     1801 cgcccgtaca tctcccgcag ccggtcgcgg atcgcccgca cccgggcccg ggtgggccgt
     1861 ttccattcct tcttcgcgac tttcgcccgc caggcagcca ccggttactg ggctccccag
     1921 tgcccgcaga ccgcggccgc cgcgagggcc gccccgggga gcacctcggc ggcgcgggaa
     1981 aggtcccagt cgagcccggc gagaccggcc gcaaggccgc ccatgaaagc gtcacccgct
     2041 ccgagcggac ttaccgtttc gaccggctcg ggacgatgcc gcgcctcgac cgcaccggcg
     2101 gtgacgattt ccccctcacc gttggtgacc acgaccagct ccgggccgag cttgaggaga
     2161 tcccgggccg cggcgagcgg ctccccgatc ccggtgatca tttccgcttc cccctggttc
     2221 agcttgacca ccgttgccga cccgctcaat tcccggcaga aatcgcgggc cggttcttcg
     2281 ctggtccagc ggttgggccg gtagttcggg tcgagcagca ccggcagccc ggccgcggca
     2341 gccagctcga ccgcgcgccg ggtcacattc cgctcgactt cgccgaccat cgtgttcgaa
     2401 ccgatgacca ccgcccggga accggtgatc gcctcttcaa ggaagtcgcc ggtggcggcc
     2461 atggtcggcc cgatgtgttc gccgtagacc tggaaagcgg gctcgtttcc ggaatcgaac
     2521 tcgatcaggg cgaccggagt gtctgccccg tcgagtgtgg ccagccatct ggttgagacg
     2581 ccttcggatt ccagcccgtc ggcgagccag cgcccccagc cgtcaccgcc aaccccgccg
     2641 accagggcgg ccggggcgcc gcttcgggcg atcgccacgg ctacgttagc cagcgcccct
     2701 cccggccacg gaacgaaggg cccgggtgct tcccccttgg ccaggttccg ctcgcagatc
     2761 agatccgcga tcgcctcgcc gagacaggcg atcgggggtc gctgatcact gtggggtcgg
     2821 gctggccgtt ctcgctccat gactggaaag ccggagccta cccgaggctt cctgagagaa
     2881 atctgcgcgc gccactagac tgagcagtca tgcccgcgct gatcgtccgg atcggggagt
     2941 ggctcccccg cggctggcct gacctctttc gccagctcct gctctatgtg ctcgccgaca
     3001 ccttctacga gatggcgcgc ggaatggccg atggccgggc caatgtcgcc ttcgccaacg
     3061 gcgaacgggt gatcgacacc gaacgcaccc tcgggatgtt cttcgaacct ggagtgcaga
     3121 gcgcctttca cggcttcacc tggctgatca acatcgccaa cacgatctac ctcaacagcc
     3181 agttcacggt ggcgctcggc ttcctgatct ggatgtacct gttccgcaac gactactact
     3241 acttcttccg caacatgctc ttcgtctcga tgggcatcgc actggtcggc tacatcggct
     3301 tcccgaccgc cccgccgcgg atgtaccccg agtacggatt cattgacacg gtcaacgccc
     3361 agtccagcgt caaccacgac tccgccctgg ccaagctctt catcaacccc ttcgccgcgg
     3421 tgccttcgat gcattgcgcg gtcgccctga tgatcggcgt caccggtttc caggtctgca
     3481 ggaactggtt cgccaagacc ttctgggcgg gctggccgct ggtggtcgcc tgggtcaccg
     3541 tggtcaccgc gaaccactac tggatcgatg ccgtgctcgg ctggggcgtc gccgggctct
     3601 ccttcctgat cgcttcgaga gtgctggccg agatcaaacc cgagacctgg gcctggagcc
     3661 gcgaggcgga gcgccaacgg gccgaggcct gagcggaggc ccggcggtgc gactccgttc
     3721 cctgaccagc ccggccgcgt ggggattcct ggccgcgccg ctgctcgtcg tggtcgggct
     3781 cgcggtgatc tacctgatca tggcgcccca cagcgccggt cacgccgccc agaccttccg
     3841 caccgagctg ttcgaactct ccgggccgac ggtctggaac aactactggt tcgccggcca
     3901 ctacctgccg tcctattccc tgctttcgcc gccgctcggg gcctggctcg gcttccgggt
     3961 gatgggttca ctggcggtga tcggaacggt gatcctcttc accctgatcg tccggcgcga
     4021 gtggggcgag cgggcccagg ccggggcgat ctggttcgcc ttcgcggcca cgatcagcct
     4081 gttttcgggg cggctcacct tcgccctcgg ttgcctgctg gccatggcgg cggtcttcgc
     4141 ctcgcagcgc ggcctgcgta tcccggcgct gctcctggcc ggctcggtcg gtctggccag
     4201 cccggtcgcc gcccttttcc tggcctgtct cggcttcgcg catttcctgg ccgagcgacc
     4261 cgaccgccgc ggtctggagc tggccggagt ctccttcctg gtcgccgcga tcgtcgcgct
     4321 gctctttccc ggcggaggcg acgagccgta tgtcacttcg agcttcatac cggcgatcgg
     4381 cctgaccctg gtcagcgcct ggatggtccc tccgggccag cgcctggtcc gcaccgggat
     4441 gctcgtctac gccggcgccc tggtcgcctc ctatgtgctc agcacgccga tgggaggcaa
     4501 cgtcaaccgg ctcggggccc tgctgctcgg gccggtcctg ctctgcgtgc tggcggccga
     4561 gccgctgacc ccgaaactgt ggcgccgcgg cgtgctgatc atcctgctgc cgatgatcgt
     4621 ctggtggcag accgggccgg tgatccggga tctcaagctg accgatgacg atccggcggt
     4681 gacccaggcc ttctaccagc ctctggccga cgccctcgag ccacgcctcg tacgcgagcc
     4741 ggccagggtc gaggtggtgc cgatcgcctc ccattgggaa gctgcccggg cggccccgga
     4801 attcccgctc gcgcgcggct gggagcggca gaccgaccgc aacttcaacc cgctcttcta
     4861 tgccgaccgg ctcggcccga accggtaccg acagtggctc gaccggctcg cggtcgggta
     4921 tgtggccctg ccggccgaca ccgaactcga ctacgccggg gagaaggagg cggccctgat
     4981 ccgctccggg cttccgttcc tcagggagat cccggtcggc ggcgactggc gactgttcaa
     5041 ggtgatcggc gcgcagccga tggtcgcccg tccggcccgc ctgaccgaac ttgacaccga
     5101 cggcttcgcg gtcgcggcgc cgatcgccgg cagcttcgag gtgagaatca ggtcgacccc
     5161 ctactggaag gtcaagagcg gatttggttg tgtcgagccg ggccggggtg actggacccg
     5221 ggtcacgctc gatcgccccg gatcgctcaa ggtcgccgcc gacttcagcc ccggggcccg
     5281 gttcggctcc gaccgggact gtcgccccga ccggtgagcc acggctccaa cctctagagt
     5341 tccccgcatg gagtcttcgt ccggcccggg aggcggccag aacggttcgc gcaggccagc
     5401 ccaggaattc cgggagcagg cccgcaacgc tctcatccag tcccggctga cccc
//
LOCUS       DAOQMF010000020         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_6745_length_5448_cov_3.221213, whole genome shotgun sequence.
ACCESSION   DAOQMF010000020 DAOQMF010000000
VERSION     DAOQMF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6745_length_5448_cov_3.221213"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /locus_tag="PLV77_00665"
     CDS             <1..1708
                     /locus_tag="PLV77_00665"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNC14392.1"
                     /translation="NLPAVGAYKVIIHLVDGALNLGRDAETILRFDDRPPGDVSPVEP
                     EGWISRDELPIQQEIEKAAPGGPSGISGYALEVSDDGPAQPCGSEICLAPEITLAGGP
                     DLRTGSIGGLVEGNHWISAAAVSGAHKSSVEPGSTMVQVDRTPPTVSISGVPNEWVNH
                     PVTLTAQASDQLSGMQPQPADDGSPKTVIDADNYAAYESPGPFASFSVATEGVNRVRY
                     WAEDLAGNANDGMPGHDGDTHATPGQAVVRIDTTPPEVRFDPARDPEDPEVVRISADD
                     TDSGVASASIGIRRAGSGETFIQLQTAGADGQYQARIPSDDLAQGAYELNATVEDRAG
                     NESTGNRTSAGDPMIVNLPVKQPASIAASLTGGKSNILARYGSARYLEGRLTSGDTAL
                     SNQTVTAIETFKTGSSRNAVSRDLITDGDGRFRVLLTPGPSRTIEVTYAGTRMISRTA
                     GPKLGMRVKGKVTLKIKPKKVLNGGVVRMSGKVGFAGVLPPSRGKLVAIQYFDPSRRK
                     WRPVEVLRTNRRGLFRYRYRFRTITSAQRILFRASALPEAGWPYLPSTTKPKSVIVYP
                     KD"
     gene            1760..2161
                     /locus_tag="PLV77_00670"
     CDS             1760..2161
                     /locus_tag="PLV77_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14393.1"
                     /translation="MVVALIALTIAIGGTAAALPGKFTVGRDDLKTSSVGARALGKMI
                     IGHSQVFPSADPTADDGNFTKSVGTITCPDKAPTAIDPFVGGLSTTAYVFGRSEIFNS
                     WGAPQGYRYTVFSDDGPEVGYVLKVNCIPAR"
     gene            complement(2305..2925)
                     /locus_tag="PLV77_00675"
     CDS             complement(2305..2925)
                     /locus_tag="PLV77_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14394.1"
                     /translation="MAEAKKTTKKSTAKKTAAKKPAAKKRTAAQLRAEAEKKAIHAAK
                     LDAENAKSRLEEVQETAVEYAKKGVDVPVGAALNLADRVSEVIEDWSDQASAEKKVKG
                     YRADLTKTVKRAERRGITTRRKATTQARKQRKSLEKTVRQQRKSIEKTVKTTNKDVNK
                     QIEARVNEVTKRANQVQADVTKVADEQGKKAQDLVNKVTEQLSALV"
     gene            complement(3098..3937)
                     /locus_tag="PLV77_00680"
     CDS             complement(3098..3937)
                     /locus_tag="PLV77_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNC14395.1"
                     /translation="MNQLPPDPALDADWTGICRHIVEEQKKLFAEYETIQQRDNYEGV
                     GEGGDHALVLDRMCEDVIFAELEKLADSGGAAFTAISEERGTVLFNEGGNVWVVIDPI
                     DGSLNFKRTIPQHSLSIAVATAPNMGAVEFGYVYDFGTGDEFLAQSGEGAYLNEERIN
                     LEPREGLEVVGIEGADPTALAPMLAELAGKVYRVRCVGSLAISIASVAAGRFDALAFP
                     HLSRSVDVAAAQLIAREAGALVDLGDGGLSEVGLALEDRFPVTVAATGEGLETLVSIR
                     TVG"
     gene            3991..>5448
                     /locus_tag="PLV77_00685"
     CDS             3991..>5448
                     /locus_tag="PLV77_00685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNC14396.1"
                     /translation="MSDSSSRTGADGDPGPGRELVRRHGSGALLIRGGAGTGKSTALR
                     ERVIHLARPETGRPGLDPCRVALIASTDRTAAAHRIRLEDDLPGPYEALSVFTWEGLA
                     EEILRHRPVEAGLGPSFEVVGAAERLAMLLARVDELPLRHQEIRGNPAGLLRELLIGI
                     DRAKREGTDGGELAELIEIHNRILAAADLLDRNELPRIATDLLADPALAAEVTARFPH
                     LVIDELEDLHPARAGLLQRLAVAGPESVVAGIDPSRAIYGPGAEERFLAAFPDLEQFT
                     LDEAWRMSEVRITAARAALGPGPLPEGAWEARGDEGSVRYWRPASARAEAQAAAREVE
                     HQIALGTPPEQLAIAVPDVNSQGVGVVGALAERGIPARPGGGAALFRQPEVRDTIAWL
                     RALTDPTDARAVTRALMRPPTELRSLDLARLTQISKRRKLDMISACEASLESPQMAPE
                     ARQRIEGFLNLYRAASGALDTRRPDAYVRRLIERIG"
BASE COUNT          928 a         1783 c         1817 g          920 t
ORIGIN      
        1 caacctgccc gcggtcggcg cgtacaaggt gatcatccat ctggtcgacg gagcgctcaa
       61 cctcggacgg gacgccgaga ccatcctccg cttcgacgac cggccgccgg gtgacgtgag
      121 tccggtcgaa ccggagggat ggatctcaag ggacgaactg ccgattcagc aggagatcga
      181 aaaggcagcg cccggtggcc cgtccggaat cagtggctac gcgctggagg tgtccgacga
      241 cgggccggcg cagccctgcg gatccgagat ctgcctcgcc ccggagatca ccctggccgg
      301 aggcccggac ctgaggacgg gttcgatcgg cggccttgtc gagggtaatc actggatttc
      361 ggcggctgcg gtctcggggg ctcacaagtc ctcggtcgag ccgggcagca cgatggttca
      421 ggtcgaccgg actccaccga ccgtctcgat atccggagtt ccgaacgaat gggtcaacca
      481 tccggtcacc ctgaccgccc aggcgagcga ccagctctcc gggatgcagc cccagccggc
      541 tgacgacggc agtccaaaaa cggtgattga tgccgacaac tacgcggcct acgaatcacc
      601 cggacctttc gccagtttct cggtggcgac cgaaggcgta aaccgggtcc gctactgggc
      661 ggaagatctg gccggcaacg cgaacgacgg gatgccggga catgacggcg acactcacgc
      721 gactccgggc caggcggtgg tccggatcga caccaccccg ccagaggtca gattcgatcc
      781 agccagggat ccggaggatc cggaagtcgt ccggatctcc gcagatgaca ccgactcggg
      841 cgtcgcctct gcttcgatcg gaatccggag ggcggggtcg ggagagacat tcattcaact
      901 ccagaccgcg ggggccgacg gtcagtacca ggccaggatc ccttccgatg acctggcgca
      961 aggcgcgtat gaactgaacg cgaccgtcga ggacagggcc ggaaacgaat cgaccggaaa
     1021 ccggacttcg gccggcgatc cgatgatcgt caacctgcct gtcaagcagc ccgcctcgat
     1081 tgccgcgtcg ctcactggcg gaaagtcgaa catcctcgcg cgctatggca gcgcccgtta
     1141 cctggaaggc aggttgacca gcggcgatac ggctttgtcg aaccagacgg tcacggcaat
     1201 cgaaacgttc aagaccggtt cgagccggaa cgcggtttcg agggatctga ttacggacgg
     1261 agacggccgc ttcagggtcc tcctcacccc gggcccgtcg cgaacgatcg aagtcacata
     1321 cgcgggcacc aggatgatct cccggaccgc cggaccgaag ctcggcatga gggtgaaagg
     1381 caaggtgacg ctgaagatca agccgaagaa agtgctcaac gggggcgtcg tccggatgtc
     1441 cggcaaggtc ggttttgccg gggtgctccc gccgtcccgg ggcaagctgg tcgcaatcca
     1501 gtacttcgac ccttcccgcc gcaagtggcg accggtcgag gtgctccgga ccaaccgccg
     1561 aggacttttc cggtaccggt accgcttccg gactatcacc tcggcccagc gaatcctgtt
     1621 cagggccagc gccctgcctg aggctggctg gccctatctg ccctcgacaa cgaagccgaa
     1681 atcggtcatt gtttacccga aggactgatt cggcagggaa ggaggcagga tgagagggat
     1741 acggagaccg tcgccggcaa tggtggtggc actgatcgcc ttgacgatcg cgatcggtgg
     1801 aacagccgct gccctgcccg gcaagttcac agtgggccgg gatgatctca agacctccag
     1861 tgtcggagcg agggcgctag ggaaaatgat cattggacat tcgcaagtct ttccctcggc
     1921 tgatcccacc gcagatgacg gcaatttcac caagtcggtc gggacgatca cctgtccgga
     1981 taaagcgcca actgcgattg atccctttgt tggcggattg agcaccaccg catacgtctt
     2041 cggcaggagt gaaatcttca attcttgggg agcacctcag ggctaccggt acaccgtctt
     2101 cagcgacgac ggacccgaag ttgggtacgt gttgaaagtc aactgcatcc ctgcccgttg
     2161 atcacatcac ttaaggacca acggccccga ggaggggccg ttggcgaggg aggagaggta
     2221 ctacatcgac tgacgaggtt atgaggtcag ccgatcttca ccggttcgcc gttctcctgc
     2281 ggcggccggg ctaagcggtt tggtctagac cagagccgaa agctgctccg tgaccttgtt
     2341 gaccagatcc tgggccttct tgccctgctc gtcggcgacc ttggtgacgt cagcctgaac
     2401 ctggttggcg cgcttggtga cttcgttcac gcgggcttcg atctgcttgt tgacgtcctt
     2461 gttggtcgtc ttgacggtct tctcgatgct cttccgctgc tggcggacgg tcttctcgag
     2521 cgacttgcgc tgcttgcggg cctgggtggt cgccttgcga cgggtggtga tgccacgacg
     2581 ctcggcgcgc ttgacggtct tggtcaggtc ggcgcggtag cccttgacct tcttctcggc
     2641 gctggcctgg tcggaccagt cctcgatcac ttcggaaacg cgatcggcca ggttgagggc
     2701 ggcgcctacc ggcacgtcaa cacccttctt ggcgtactcg acggcggtct cctggacctc
     2761 ttcgaggcgg ctcttggcgt tctcggcgtc gagcttggcg gcgtggatgg ccttcttctc
     2821 ggcctcggcg cggagctggg cggcggtgcg cttcttggca gccggcttct tcgcggcggt
     2881 cttcttggcg gtgctcttct tggtggtctt cttggcttcg gccatgggga tcagttagtc
     2941 ctttgtttgt tgagatcaga ttacttactt ctaaggatgt ttgaagtgta gcaaccaaac
     3001 acgaacacat gttctttttt cgtgtgacct cggtcacgca gggagaatcc gcatggcaga
     3061 gcggaaaaca gcgccggaat cggacgcaat ccgggattca gccgacggtg cgaatcgaaa
     3121 cgagggtctc cagaccctcg ccggtggcgg ccacggtgac cgggaaacgg tcctcgaggg
     3181 cgaggccgac ctcgctcaga ccgccgtccc cgaggtcaac cagggcgccg gcctcgcggg
     3241 cgatcaactg ggccgccgcc acgtcaaccg agcgggagag atgggggaag gcgagcgcgt
     3301 cgaaacggcc cgcggcaacc gaagcgatcg agatcgccaa cgagccgaca cagcgcaccc
     3361 ggtagacctt gccggccagt tcagccagca tcggggcgag tgcggtgggg tcggccccct
     3421 cgatgccgac cacctcgagc ccctcccggg gctccaggtt gatccgctcc tcgttcaggt
     3481 aggccccctc gccggactgg gcgaggaact cgtccccggt cccgaagtca tagacatagc
     3541 cgaactcgac cgcccccata ttcggggcgg tggcgaccgc gatcgaaagg ctgtgctgcg
     3601 ggatcgtccg cttgaagttg agcgaaccgt cgatcgggtc gatcacgacc cagacgttgc
     3661 cgccctcgtt gaacaggact gtcccgcgtt cctcggagat cgccgtgaac gcggcaccgc
     3721 cgctatcggc cagcttctcg agttcggcga agatcacgtc ctcgcacatc cggtcgagga
     3781 ccagggcgtg gtcgcctccc tcgccgacgc cttcgtagtt gtcgcgttgc tggatcgtct
     3841 cgtactcggc gaagagcttc ttctgttcct cgacgatgtg gcggcagatc cccgtccagt
     3901 cagcgtcgag tgcggggtcc gggggaagtt ggttcacccg cgacatgcta gacgctgtga
     3961 aacaggacga ggctcgggta atttgagggg gtgagcgatt cctcgtccag aaccggtgcc
     4021 gacggagatc ccggccccgg ccgggaactg gttcgcaggc acggctccgg ggcgctcctg
     4081 attcggggcg gcgcgggtac cggcaagagc accgccctcc gggaacgggt gatccacctc
     4141 gcgcggccgg aaaccggccg gccgggcctc gatccgtgcc gggtcgccct gatcgcctcg
     4201 accgaccgca ccgccgccgc ccaccggatc cggctcgaag atgatttgcc cgggccctat
     4261 gaagcgcttt cggtcttcac ctgggagggc ctggccgaag agatcctccg ccaccggccg
     4321 gtcgaggcag ggctcggacc ctccttcgag gtggtcggcg cggccgaacg cctggccatg
     4381 ctgctggccc gggtcgacga gctgccgctc cgccaccagg agatccgggg caacccggcc
     4441 gggctgctcc gggagcttct gatcggcatc gaccgggcca aacgggaggg caccgacggc
     4501 ggcgagctgg ccgaactgat cgagatccac aaccggatcc tcgccgcggc cgacctgctc
     4561 gaccgcaacg aactgccgcg catcgccacc gacctgctgg ccgatccggc cctcgccgcc
     4621 gaggtcacgg cccgcttccc ccacctggtg atcgacgagc tcgaggacct tcacccggcc
     4681 cgggccgggc tgcttcagcg actggccgtg gccgggcccg aaagcgtggt cgcgggcata
     4741 gacccctccc gggcgatcta cgggccgggc gccgaggaac ggttcctggc cgccttcccg
     4801 gacctggagc agttcaccct ggatgaggcc tggcggatga gcgaggtgcg gatcaccgcc
     4861 gcccgggccg cacttggtcc gggccccctg cccgaaggag cctgggaagc tcgcggcgat
     4921 gaaggctcgg tccgctactg gcggcccgcc tcggcccggg cggaggcaca ggccgcggcc
     4981 agggaggtcg aacaccagat cgccctggga actccgccgg agcagctggc gatcgcggtg
     5041 ccggacgtga actcgcaagg agtcggggtg gtcggggcgc tggccgaacg gggcatcccc
     5101 gcccggcccg gcggtggcgc cgcgcttttc cggcagccgg aggtgcgcga cacgatcgcc
     5161 tggctgaggg cgctgaccga tccgaccgac gcccgggcgg tcacccgcgc cctcatgcgg
     5221 ccgccaaccg agctccgctc cctggacctg gcccggctca cccagatctc gaaacgacgc
     5281 aagctcgaca tgatcagcgc ctgcgaggcc tcgctcgaat caccgcagat ggcgccggag
     5341 gcccggcaac ggatcgaagg cttcctcaac ctctaccggg cagcctcggg cgcgctcgac
     5401 acccgccggc ccgacgctta tgtgaggcgg ctgatcgagc ggatcggt
//
LOCUS       DAOQMF010000021         5390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_6874_length_5390_cov_4.053796, whole genome shotgun sequence.
ACCESSION   DAOQMF010000021 DAOQMF010000000
VERSION     DAOQMF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6874_length_5390_cov_4.053796"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="PLV77_00690"
     CDS             complement(<1..1712)
                     /locus_tag="PLV77_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNC14397.1"
                     /translation="MAHDYDYVIVGSGFGGSVSALRLAEKGYKVAVIETGRRFEDKDF
                     AKTAWNMRRYFWLPKFGLRGILRMSMFKDVFVVSGCGVGGGSLGYANTLYRPRPAFYT
                     DPQWDGLADWEAELGPHYVTAERMLGVTLYRDTGPADALLHEYADEIGADKTYSNTQV
                     GVFFGEAEKTVPDPYFGGEGPERTGCSKCGSCMVGCRHGAKNTLVKNYLFFAEKLGVK
                     VFPGTEVTSVRPIGGPDGEHRDGSAGYELRTDRSGAVLRHRRRKLTAAKVIFSAGALG
                     TNKLLANCLHNGDLPNLSKRVGHVVRTNSESIQAVTAHDDERDFSDSVAITSSIYPDP
                     DTHIEVVTYGQAGDVMSRLYTIYTGNGTRLTRPIRFLATMARHPIDTLHLLFMPRHWS
                     RRTVILLVMQALDSAMRLKPVPKKFGKGVRLQTEQDPERPNPTFIQAAEDATKWFAKR
                     TGGIPQVSFGESLFNIPTTAHILGGAVVGHSPEDGVIDKDNHVFGYENLMVCDGSAVP
                     ANPGVNPSLTITAMTERAMSKIPPKDPAAELLHLPDEARPARTADTVGPARGPGPSGS
                     AESWT"
     gene            1946..3655
                     /locus_tag="PLV77_00695"
     CDS             1946..3655
                     /locus_tag="PLV77_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14398.1"
                     /translation="MKQRLAVIVGILAAFLVGAFAVTAPASAADAGAVKLQKQVKAAQ
                     KKATATCKKVKKAKKGKSKAKAKKRCKSAKQNVTRLQKKLKAYNAAQYFDVCKHGCKY
                     RTVQKGADAAGLWQKKSKRKATVRVKPGTYVEGVFLDGRNPKYDYDGLTIMGTSKKAK
                     DVILEGENAKTIVDGVPQVANNAIEGRSIIGLVMKNMWARHYQNNTFFVWAGHDGTER
                     CADYTMDNLVSSDTRSYGFFARNCFGGVIENSSGYHHGDSAVYIGETPCDSPDWTNRT
                     VAAERTPCQADPDWTIIRNVKSYANVLGYSGTNSKYVDIKNSAFYNNGAGIVPNTLDS
                     EQFEPSGWLKIHNNDIFWNNYNYYSTGSDFQTVATIGDVNYPIGIGVVLYGTDSVEVY
                     DNNIFGNEKWGAATFSGPELFGVNVGDDAMNLNNKFIDNNMGRNGDDPNGKFDFFSDY
                     SGGGNCWQGNTAGSSFAPGNGTVALSTIYPSSCPQPVVGQKDVSPIELTAGLQINIAS
                     YADDGDPSNGPGDGDTIFGYVSQHPPQKQECEMNVSTPHPAYTDAKGVSYTEDRADPV
                     TCS"
     gene            3709..4272
                     /locus_tag="PLV77_00700"
     CDS             3709..4272
                     /locus_tag="PLV77_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNC14399.1"
                     /translation="MSSKIKSRAAGLLSVGIVALALGAASAVPATASASGDGAASSSS
                     LTAKQKKAKRAELKKCYRIKAKAKRKACIKRVNKKYAKLAKPPVKVAATIDVLDDYYY
                     PSDVTINRGEAVKWVWSNSNANGHTVSLDDPFPVGLTASEKYSLNTPTAPATFYTFGP
                     KQLKKPGVYHFVCELHSATMKMTVTVK"
     gene            4319..5344
                     /locus_tag="PLV77_00705"
     CDS             4319..5344
                     /locus_tag="PLV77_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC14400.1"
                     /translation="MDRRNFLGLAGGAFLCTIAGKQYSIDSNTDLAALANKIEVPPKV
                     AAAKRSNSVSNADISGNRKEYWIQAEPVTWNVVPKQRDQMMGTSIKKTVGRTKVPAYA
                     YRLYDTDFSKPLGPATVPGPLLDVTVGDTLVVNFRNACDVPVTMHPHGVFYSNEMDGA
                     YKGYWTDPGGFVQKGRTFQYVWECPEGTQGSWLYHDHGPFDSTAVFKGLFGPLIVRDP
                     DEPLPDREYFLGFHSYLPGLFPLKAPLFAINGRAYAGNTPTMRATVGDDVAMHVYGVD
                     NDMHTFHVHGHRWQEAGGTYADNKIFGPGDSFRVRWIEQNPGRWLYHCHVFSHLVTGM
                     SGWYIVD"
BASE COUNT         1065 a         1713 c         1668 g          944 t
ORIGIN      
        1 gtccaggatt cggccgaccc ggaggggccg ggcccgcggg ccgggccgac tgtgtcggcg
       61 gtccgggccg ggcgggcctc gtccggcagg tgaagcagtt cggcggcggg atccttaggc
      121 gggatcttgc tcatcgcccg ttcggtcatc gcggtgatcg tcaggctcgg gttgactccc
      181 ggattggccg gcacggccga gccgtcacag accatcaggt tctcgtagcc gaagacgtgg
      241 ttgtccttgt cgatcacccc gtcttcgggg ctgtggccga ccacggcacc gccgaggatg
      301 tgggcggtgg tcgggatgtt gaagagcgac tcgccaaagg aaacctgggg gatgccgccg
      361 gttcgcttcg cgaaccactt ggtcgcgtcc tcggcggcct ggatgaaggt cgggttgggc
      421 cgttccgggt cctgctcggt ctggagccgg actcccttgc cgaacttctt cgggaccggc
      481 ttgagccgca tcgccgagtc gagcgcctgc atgaccagga ggatcacggt tcgccttgac
      541 cagtgccggg gcatgaacag caggtgaagg gtgtcgatcg ggtggcgtgc catcgtggcc
      601 aggaacctga tcggccgggt caaccgggtc ccgttgccgg tgtagatcgt gtagaggcgg
      661 ctcatcacat ccccggcctg gccgtaggtg acgacctcga tgtgagtgtc ggggtcgggg
      721 tagatgctgg aggtgattgc caccgaatca ctgaagtctc tttcgtcatc gtgcgcggtc
      781 acggcctgga tcgactccga gttggtccga accacatggc cgaccctctt cgagaggttc
      841 ggcaggtcgc cgttatggag gcagttggcg agcagtttgt tggtgccgag ggctccggcc
      901 gaaaagatca ccttggcggc cgtcagtttc cggcgccgat gccgcagcac ggcaccagag
      961 cggtcggttc tcagctcgta gcccgcggag ccgtcccgat gctcgccgtc gggcccgccg
     1021 atcggcctga ccgaagtgac ctcggtgccg gggaacacct tgacccccag cttctcggcg
     1081 aagaagaggt agttcttgac cagggtgttc tttgctccgt gccggcaacc gaccatgcat
     1141 gaaccgcact tgctgcagcc ggtgcgctcc gggccctcgc cgccgaagta gggatcgggg
     1201 acggtcttct cggcttcacc gaagaagacc ccgacctggg tgttcgagta ggtcttgtcg
     1261 gcgccgatct cgtcggcgta ttcgtgaagc agcgcgtcgg ccggaccggt gtcgcggtaa
     1321 agcgtcacgc ccagcatccg ctcggcggtc acgtaatggg ggccgagctc ggcttcccag
     1381 tcagccaggc catcccactg gggatcggtg tagaaggcag gccggggccg gtagagggtg
     1441 ttggcgtaac cgaggctgcc gcctcccacg ccgcaaccgg aaacgacgaa gacgtccttg
     1501 aacatcgaca tcctcaggat cccgcgcagg ccgaacttcg gcagccagaa gtaacgccgc
     1561 atgttccagg ccgtcttcgc gaagtccttg tcttcgaagc ggcggccggt ctcgatcacg
     1621 gccaccttgt agcccttttc ggccagtctc agggcggaaa cactgccgcc gaatcccgaa
     1681 ccaacgatca cgtagtcgta gtcgtgcgcc atggcaatca agtgtaaccg acatgaagcc
     1741 ttgtcaattt ctcgcatggc agtgcggatt gctcggcccc tggagtcgga caggaagcgg
     1801 aaatcgcgtc agagcgccaa ttcatccgat ccgggttaag cgcaggaccg caacctttcc
     1861 gttaagggtg gtttagagtg tccggatggc ttttccgcat tcctgcggtc gagccgcaca
     1921 cacttttcac acttcaagga gagagatgaa gcaacgtttg gctgtaatcg tcggcattct
     1981 tgccgcgttc ctggtgggcg ctttcgccgt caccgctcca gcttcggcgg ctgacgccgg
     2041 tgctgtgaag ctgcagaagc aggtcaaggc cgcgcagaag aaggccacgg ctacctgcaa
     2101 gaaggtcaag aaggcaaaga agggcaagtc gaaggccaag gccaagaagc gctgcaagtc
     2161 cgccaagcag aacgtgactc gtctccagaa gaagctgaag gcctacaacg ccgcccagta
     2221 cttcgacgtc tgcaagcacg gctgcaagta ccgcaccgtc cagaagggcg ctgacgcggc
     2281 cggcctgtgg cagaagaagt ccaagcgcaa ggcgaccgtg cgagtcaagc ccggcaccta
     2341 cgttgagggc gtcttcctcg atggtcgtaa ccccaagtac gactacgacg gcctgaccat
     2401 catgggcacc tcgaagaagg cgaaggacgt catcctcgag ggtgagaacg ccaagacgat
     2461 cgtcgacggt gttccccagg tcgccaacaa cgcgatcgaa ggccgcagca tcatcggcct
     2521 cgtcatgaag aacatgtggg cccgccacta ccagaacaac acgttcttcg tctgggccgg
     2581 tcacgacggc accgagcgtt gtgccgacta caccatggac aacctcgtct cctccgacac
     2641 ccgttcctac ggcttcttcg cccggaactg cttcggtggt gtgatcgaga actcctcggg
     2701 ctaccaccac ggtgactccg ccgtttacat cggtgagacc ccgtgtgatt cgcctgactg
     2761 gaccaaccgg accgttgccg cagagcgcac gccctgccag gccgatccgg actggaccat
     2821 catccggaat gtcaagagct acgccaacgt cctcggctac tcgggcacca actccaagta
     2881 cgtcgacatc aagaactcgg cgttctacaa caacggtgcc ggcatcgtgc cgaacaccct
     2941 cgactccgag cagttcgagc cgagcggctg gctgaagatc cacaacaacg acattttctg
     3001 gaacaactac aactactact cgaccgggtc tgacttccag acggttgcca cgatcggtga
     3061 cgtcaactac ccgatcggca tcggcgtcgt cctgtacggc accgacagcg tcgaggtcta
     3121 cgacaacaac atcttcggca acgagaagtg gggcgcagcc accttctccg gaccggaact
     3181 gttcggtgtc aacgtcggcg acgacgcgat gaacctgaac aacaagttca tcgacaacaa
     3241 catgggccgc aacggcgatg atccgaacgg gaagttcgac ttcttctcgg attactcggg
     3301 cggtggcaac tgctggcagg gcaacactgc cggttccagc ttcgcccccg gcaacggtac
     3361 ggtcgcgctt tccacgatct acccgagcag ctgcccgcag ccggtggttg gtcagaagga
     3421 cgtttcgccg atcgaactga cggccggcct ccagatcaac atcgcctcct acgccgacga
     3481 cggtgacccg tcgaacggcc ccggtgacgg tgacacgatc ttcggatacg tgtcgcagca
     3541 cccgccgcag aagcaggagt gcgagatgaa cgtcagcacc ccgcatccgg cgtacaccga
     3601 tgccaagggt gtctcgtaca ccgaggatcg ggccgacccg gtgacctgct cctgaccagg
     3661 agcagttctg cccccagcca aaccacaatt caagaggtaa gaatcaaaat gagcagcaag
     3721 atcaagtcaa gagcagcagg gttgctgtcc gtgggaatcg tcgcgctggc gctgggggcc
     3781 gccagcgcgg ttcccgccac tgcttcagca tcgggtgacg gagccgcgtc cagctccagc
     3841 ctgaccgcga agcagaagaa ggccaagagg gccgaactga aaaagtgcta caggatcaag
     3901 gcgaaggcga agcgcaaggc ctgcatcaag cgggtcaaca agaagtacgc gaagctcgcc
     3961 aagcctccgg tcaaggttgc cgccacgatt gacgtcctcg acgattacta ctacccgtcc
     4021 gatgtgacca tcaaccgggg cgaggccgtc aagtgggtgt ggagcaactc gaacgcgaac
     4081 ggtcacaccg tttccctgga tgatccgttc ccggtcgggc tgaccgccag cgagaagtac
     4141 tcgttgaaca ctccgaccgc gccggccacc ttctacacct tcggccccaa gcagctgaag
     4201 aagccgggcg tctatcactt cgtctgtgag ctccactcgg cgacgatgaa gatgacggtc
     4261 acggtcaagt aaccgggcga gcatcaggtc tggcaagagc cagaaaggac tggatccaat
     4321 ggaccgcagg aatttcctcg gactggccgg tggtgcgttt ctctgcacca tcgcgggcaa
     4381 gcagtactcg attgacagca acaccgacct cgccgctttg gcgaacaaga tcgaggtacc
     4441 gccgaaggtt gcggctgcaa aacggagcaa ttccgtaagc aacgccgaca tctccggcaa
     4501 ccgcaaggag tactggatcc aggccgaacc cgtcacctgg aacgtggtcc ccaagcaacg
     4561 ggaccagatg atgggtacgt cgatcaagaa gacggtcggg cggaccaagg tcccggccta
     4621 tgcctaccgt ctttacgaca ccgacttcag caagccgctg ggtccggcca ccgtgccggg
     4681 cccgctgctt gacgtcacgg tcggtgacac cctggtcgtc aacttccgca acgcctgcga
     4741 cgtcccggtc accatgcacc cgcacggcgt cttctactcg aacgagatgg acggcgccta
     4801 caagggctac tggaccgacc cgggcggatt cgtccagaag ggccgcacct tccagtacgt
     4861 ctgggaatgc cccgaaggca cccagggcag ctggctctac cacgaccacg gacctttcga
     4921 ttcgaccgcg gtcttcaagg gccttttcgg gccgctgatc gtccgtgacc cggacgagcc
     4981 gctgccggac cgcgagtact tcctcggttt tcattcgtac ctgcccggcc tgttcccgct
     5041 gaaggccccg cttttcgcga tcaacggccg tgcctacgcc ggcaacacgc cgaccatgcg
     5101 ggccacggtt ggtgacgacg tcgcgatgca tgtttacggc gtcgacaatg acatgcacac
     5161 tttccacgtc cacggtcacc gctggcagga agcgggtggc acctacgcgg acaacaagat
     5221 cttcggcccg ggtgactcct tccgggtccg gtggatcgag caaaaccccg ggcgctggct
     5281 ctaccactgc catgtgttca gccatctcgt gacgggaatg agcggttggt acatcgtcga
     5341 ctgacgtcag gcgccggccg ggcgctccgg gcgctcatcg ccgtatttgc
//
LOCUS       DAOQMF010000022         5249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_7163_length_5249_cov_4.064690, whole genome shotgun sequence.
ACCESSION   DAOQMF010000022 DAOQMF010000000
VERSION     DAOQMF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7163_length_5249_cov_4.064690"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLV77_00710"
     CDS             <1..362
                     /locus_tag="PLV77_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14401.1"
                     /translation="AMTGYACAFYYRRELFKSVKNLLFVGLAPVIGALLLTYLFIKSL
                     VDWSDPANSYSGASWFGFAPPAVIGVGFLLLGAMLLLAWRIHQEEPFFKRRREVADPG
                     LLEGKAGAAVALAGPEN"
     gene            complement(359..1840)
                     /locus_tag="PLV77_00715"
     CDS             complement(359..1840)
                     /locus_tag="PLV77_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14402.1"
                     /translation="MKTSADHKLPRLARCLALLAMLVALACFGGRADAAVTGDNPCPT
                     SASNSLSWMGASVGQDFTIPSLRHQADSSQVPATYPMTVLRPADTVTYPGPRPVVVFQ
                     HGLGGNRCALWWAAQYLAGHGFVTTVHRSLPGNEQTASDAYRSGVDAVRSVIAYLKGL
                     QVSDPNLGPMIDLGRLGLSGHSMGSIISSQLQGDPSLGIKAIVAFDTLRRYVQGDPGA
                     ARIDCQSDPVPATQVTPSVPALSFAMDEPCAYKPDFKPADLKLSGPRWWSEHGVPNMQ
                     LVMKGYPHGGFTDGASADKKLDLAHWVLPWFERWLNGDKSQEQLLAAPIVNGRPVVDL
                     LSSEFLSSACLDGIADSEDLRSWFNAGSPATSGAGRCDSPPGQSPDPAHPAPALTNRK
                     LVKLVRLSSSRGWVRAGGKARLKLRITVKGMGQPVKNLNLLVSSSNRRVRVAGTRKVQ
                     RINPASTRVVVLTVRAGRKARNRVRVKVTVAGRTVGRTLRIRR"
     gene            2074..3297
                     /locus_tag="PLV77_00720"
     CDS             2074..3297
                     /locus_tag="PLV77_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC14403.1"
                     /translation="MEGSPDLPPIDPDADQTEAVAGNAARSGSGGVRLEGVTKKFGDF
                     TAVSEMDLDIADGEFFTMLGPSGCGKTTTLRMVAGFEQPTTGKVLIDGTDVAGQPAYK
                     RPTNTVFQSYALFPHMTLEENVGFGLKRKKVPKAEIKQRVANELERVGLSREAKRKPN
                     QLSGGQQQRVALARALVNLPKVLLLDEPLGALDLKLRKGLQVQLKQIQRDVGITFVYV
                     THDQEEALTMSDRIAVMNEGRIEQCADPETVYDRPATTFVAGFIGVSNLMPAEVKGSN
                     EIQVVNGPLVPTPDAAKYSGGEKCFAVVRPEKLTVSLPENAPSDQPGVDGIVESSLYL
                     GTSTQMAVRVTEDVMMTVLVPNASEDARQHLPGGGAKVRLNWLPEHMHLVAESESATA
                     ATDPTPTEKVTEGVN"
     gene            3297..4451
                     /locus_tag="PLV77_00725"
     CDS             3297..4451
                     /locus_tag="PLV77_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC14404.1"
                     /translation="MSESNVKRVRLLALAVLALAVSMVLVACGGGGIDEGSDNSDVTT
                     ATGGDPQGSLKISNWPLYIDGATIKDFQNDTGINTTYTEDVNDNNEFFGKVQQQLAQG
                     DSGGRDIIVVTDWMAEKMYNLGYIQNLDKAKLPTVEKNLIPSLQSPSFDPERKYSVPW
                     QSGMTGLVVRKDLAPDVKSINDLFDPKYKGKVTLLTEMRDTVPLVMAADGVDPKEATT
                     QDWLDAIDKIKENVDNGQIRRFTGNDFVQDLAKGDVVAAIGWSGDAVQAQSDNENIEY
                     VQPEQGCSIWSDNMLIPVGAPNPAAAYKFMNYVYEPENQAQITKYVNYVSPVKGVREI
                     LEKQDPAIANNNLIFPSEQFTKNCFAQVSPPGDEAAQQEVEQAFQDVVTG"
     gene            4518..>5249
                     /locus_tag="PLV77_00730"
     CDS             4518..>5249
                     /locus_tag="PLV77_00730"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14405.1"
                     /translation="MTIRRRLIPYWLLAPGLIWLVLFFVIPMYYMGELSLKTGSLETG
                     YQLTWAFENYTDSISEYSTQFIRAFWYAGVATFFCLLIGYPLAYAIAFKAGRWKNLML
                     FAVVAPFFTTYLIRTIAWKTILDDSSPVVSFLKTIHLVPQDGRVLATSAAVIAGLTYN
                     FLPFMVLPLFTALERLDQRLLEAGKDLYASATQTFWRITLPSIRWGLTYGIVLTVART
                     LGEYGAVIMVSSNLPGKSQTLTLLVS"
BASE COUNT         1014 a         1701 c         1632 g          902 t
ORIGIN      
        1 acgcgatgac cggttacgcc tgtgccttct actaccgccg ggagctcttc aagtcggtga
       61 agaacctgct cttcgtcggc ctcgccccgg tgatcggagc cctgctgctc acctacctct
      121 tcatcaagtc gctggtcgac tggtccgatc cggccaattc ctacagcggc gcctcctggt
      181 tcggcttcgc cccgccggcg gtgatcgggg tcggcttcct gctgctcggc gcgatgctcc
      241 tgctggcctg gcggatccat caggaagagc cgttcttcaa gcggcgccgc gaggtcgccg
      301 acccggggct gctggagggc aaggccggag cggcggtcgc cctggccgga ccggaaaact
      361 agcgccggat ccgcagcgtc ctgccgacgg ttctgccggc cacggtcacc ttgacccgga
      421 ccctgtttcg ggctttcctt ccagccctca cggtcagcac gaccacacgg gtgctggccg
      481 gattgatccg ctgaaccttc ctcgtgccgg cgacgcgcac gcgccggttc gaggagctga
      541 ccagcaggtt caggttcttc accggctggc ccatgccctt gacggtgatc cgcagtttca
      601 atctggcctt gccgccggcc cggacccagc cgcgggatga actcagcctg accagcttga
      661 ccagcttgcg attggtgagg gcgggagcgg gatgggcggg atccggtgac tggccgggcg
      721 gtgaatcgca gcggccggct ccgctggtgg ccggcgaacc ggcgttgaac cagctccgca
      781 gatcctcgct gtccgcgatc ccgtcaagac aggcggaaga aaggaactcg ctgctgagca
      841 ggtccacgac cggcctgccg ttgacgatcg gggcggcgag gagctgttcc tggctcttgt
      901 caccgttcag ccagcgttcg aaccagggca gaacccagtg ggccagatca agtttcttgt
      961 cggccgaggc gccatcggtg aacccgccgt gcgggtaacc cttcatgacc agctgcatgt
     1021 tcggaacccc gtgttcggac caccaacggg ggccgctcag tttcaggtcg gccggtttga
     1081 agtccggctt gtaggcgcag ggctcgtcca ttgcgaagct gagcgccgga acgctcgggg
     1141 tgacctgggt ggccgggacg ggatcactct ggcagtcgat ccgggccgcg ccgggatccc
     1201 cctgcacgta tcggcggagg gtgtcaaagg cgactatcgc cttgatgccc agggagggat
     1261 ccccctggag ctggctcgaa atgatcgatc ccatcgaatg cccggaaagt ccaagccggc
     1321 ccaggtcgat catcggcccg aggttcgggt ccgagacctg gaggcctttg aggtacgcga
     1381 tcaccgagcg gactgcgtcg acaccgctgc ggtaggcatc ggaagcggtc tgctcgttac
     1441 ccggcaacga gcggtgaacc gtggtcacga agccgtgacc ggccaggtac tgggccgccc
     1501 accagagcgc acagcggttg ccgccgagcc cgtgctgaaa gacgactacc gggcggggcc
     1561 cgggataggt caccgtgtcg gcggggcgga gcacggtcat cgggtaagtc gccggaacct
     1621 ggctggaatc ggcctgatgg cgaagcgagg ggatcgtgaa gtcctgcccg accgaagctc
     1681 ccatccacga aagcgaattc gaggcagaag tcgggcaggg attgtcaccg gtcaccgcag
     1741 cgtcagcccg gccgccgaaa caggcaaggg cgacgagcat ggccagcagg gcgaggcatc
     1801 tcgccaggcg gggaagcttg tgatcggcag aggttttcac gggtactaat cccaacaccg
     1861 agaccgccgc gagttgcgat ccggtcgcgc ctgcgaaagc cgtgcataga ggcagtcgcg
     1921 aagccgcgga tatggacaat gggcacattc gatatgtccc gtttggacaa aacgtggttg
     1981 cggaggccac ccgttttcgc atacggttca accgaacttg ccggaggagg cgttctgaaa
     2041 ctccggttct tgtccagctg agagaggggt tcgatggaag gctcgccaga tctaccgccg
     2101 atcgatccgg atgcggatca gaccgaggcc gtagccggga acgccgcgag atctgggagc
     2161 gggggagttc gcttggaggg agtcaccaaa aagttcggtg atttcacggc agtaagtgag
     2221 atggatctcg acatcgccga cggcgagttc ttcaccatgc tcggtccctc cggctgcggc
     2281 aagacgacca ccctgcggat ggtcgccggc ttcgaacagc cgacgacagg caaggtcctg
     2341 atcgatggca cggatgtcgc cggccaaccg gcctacaagc gcccgaccaa caccgtcttc
     2401 cagtcctacg cgctttttcc ccacatgacc ctggaggaga acgtcgggtt cggtctcaag
     2461 cgcaagaagg tgcccaaggc cgaaatcaag cagcgtgtag cgaacgaact cgagagggtc
     2521 ggcctttccc gcgaggcgaa gcgcaaaccg aatcagctct ctggcggcca gcagcagcgc
     2581 gtcgccctgg cccgcgccct ggtcaacctg cccaaggtgc tcttgctcga cgagccgctt
     2641 ggcgctctcg acctcaaact ccgcaaaggc ctccaggtcc agctcaagca gatccagcgc
     2701 gacgtcggca tcaccttcgt ctacgtcacc catgaccagg aagaagccct gaccatgagt
     2761 gaccggatcg cggtcatgaa cgaggggcgc atcgaacaat gcgccgatcc ggagacggtc
     2821 tacgaccgac cggccacaac cttcgtggcg ggcttcattg gcgtttccaa cctgatgccc
     2881 gccgaagtca agggatcgaa cgaaatccag gtcgtgaacg gccccttggt cccgaccccc
     2941 gacgccgcga agtactccgg tggcgagaag tgttttgcgg tagtgcgtcc ggagaagctg
     3001 acggtttcgc tccctgagaa cgcgccatcc gaccagcccg gcgtcgacgg aatcgtcgaa
     3061 agctcgcttt acctcggcac ctccacccag atggccgtgc gggtcaccga ggacgtgatg
     3121 atgaccgtgc ttgtcccgaa cgcgtccgaa gatgcccggc agcacctccc gggcggcggc
     3181 gcaaaggttc gcctcaactg gctgcccgag cacatgcacc tcgtggccga gtccgaatcg
     3241 gccacggccg ccaccgaccc aacgcccacc gaaaaagtca ccgaaggagt caactgatgt
     3301 ccgagagcaa tgtgaaacgc gttcgactgc tggccctggc cgtcctggcc ctggccgtct
     3361 cgatggtcct cgtcgcctgc ggcggcggcg gcatcgacga aggctctgac aactctgacg
     3421 tgaccaccgc aaccggcggc gatccgcagg gatcactgaa gatttcgaac tggcccctct
     3481 acatcgacgg ggccaccatc aaggatttcc agaatgacac cggcatcaac acgacttaca
     3541 cagaggatgt caacgacaac aacgagttct tcggcaaggt ccagcagcag ctcgcacagg
     3601 gcgactcggg aggccgcgac atcatcgtcg tgaccgactg gatggccgag aagatgtaca
     3661 acctcggtta tatccagaat ctcgacaagg ccaagcttcc caccgtcgag aagaacctga
     3721 tcccgagcct ccagagtccg tcgttcgacc ccgaacgcaa gtacagcgtg ccgtggcagt
     3781 ccggcatgac cggcctggtc gtccgcaagg atctcgctcc ggacgtgaaa tcgatcaatg
     3841 acctcttcga cccgaagtac aagggcaaag tcaccctgct gaccgagatg cgggatacgg
     3901 tgcccctggt catggccgcc gacggggtcg accccaagga ggcgactacc caggactggc
     3961 tcgacgcgat cgacaagatc aaggagaacg tcgacaacgg gcagatccgc cgcttcaccg
     4021 gcaacgactt cgtccaggac ctggccaagg gcgatgtggt cgcggcgatc ggctggtccg
     4081 gtgacgcggt ccaggcccag tccgacaacg agaacatcga gtacgtccag cccgagcagg
     4141 gctgttcgat ctggtccgac aacatgctga tcccggttgg ggcgcccaac ccggccgcgg
     4201 cctacaagtt catgaactac gtctatgagc ccgaaaacca ggcgcagatc accaagtacg
     4261 tgaactacgt aagtccggtc aagggcgtaa gggagatcct tgagaagcag gatcctgcga
     4321 tcgccaacaa caacctgatc ttccccagtg agcagttcac caagaactgc ttcgcgcagg
     4381 tctcaccgcc cggcgacgaa gccgcgcagc aggaagtcga gcaggccttc caggacgtgg
     4441 tcacaggctg accccacgaa acagcacaga aatcaggatg aagaagccgg cggatcaatg
     4501 agaaagagtt ccaactgatg acgatccgcc ggcgtctgat tccttactgg ctgctcgccc
     4561 cgggcctgat ctggctcgtt ctcttcttcg tgatccccat gtattacatg ggggagctct
     4621 cgctgaagac cggctcgctc gaaaccggat accagctcac ctgggcgttc gagaactaca
     4681 ccgactcgat cagcgagtac tcgacccagt tcatcagggc cttctggtac gccggcgtcg
     4741 cgactttctt ttgccttctg atcggctacc cgctcgctta cgcgatcgcc ttcaaggcgg
     4801 ggcgctggaa gaacctgatg cttttcgcgg tggtggcacc cttcttcacc acctacctga
     4861 tccggaccat cgcctggaag accatccttg atgactccag cccggtagtc agcttcctca
     4921 agacgattca cctcgtgcct caggacgggc gggtcctggc cacatcggct gcggtgatcg
     4981 ccggcctcac ctacaacttc ctgcccttca tggtcctgcc gctcttcacc gctctcgagc
     5041 ggctcgacca gcggctgctt gaggccggca aggatttgta tgcgtcagcc acgcagacgt
     5101 tctggcggat cacgcttccc tcgatccggt gggggctgac ctacggcatc gtcctgaccg
     5161 tggcccgcac gctcggcgag tacggcgcgg tgatcatggt gtcgtcgaac ctgcccggga
     5221 agtcccagac gctgaccctt cttgtctcc
//
LOCUS       DAOQMF010000023         5148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_7400_length_5148_cov_3.785195, whole genome shotgun sequence.
ACCESSION   DAOQMF010000023 DAOQMF010000000
VERSION     DAOQMF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7400_length_5148_cov_3.785195"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..685
                     /gene="pgl"
                     /locus_tag="PLV77_00735"
     CDS             11..685
                     /gene="pgl"
                     /locus_tag="PLV77_00735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNC14406.1"
                     /translation="MVARFVEDEESAAAEAARIVELAAEAAIADRGRFNFAVSGGKTP
                     WRMLELLAESDLNWTRTSLFQVDERIAPTGSMQRNLTHLVLTLPLICQAAIRPMPVGA
                     DDLATAASGYEYSLPDRFDLIHLGMGTDGHTASLVPGDPILEVSDRQVAITSGEYQGT
                     RRMSLTYKALDRARQILFLVTGDDKEEALAKLVDSDPSIPAGRLKNPNVTLITDIDIR
                     DSEPVS"
     gene            700..1350
                     /locus_tag="PLV77_00740"
     CDS             700..1350
                     /locus_tag="PLV77_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNC14407.1"
                     /translation="MGAERYGFALLLILASMIFIMATHDGKWSNVISLNLQAFALFAS
                     TRAAQPRMAIRVTVGVIIGLVLVAAWIQAGFTSSVNDDFVRLSTFSLVVIATPAIAWG
                     LVRQVKARGMITTHTMLGVLCIYLLMSLAFSAAFGAISAVSGDPFFQQGAADGTTNNY
                     LYYSLTTITTVGMGDFTPATNLGRSLTAAEALIGQIYVITVVAVIVGNLGRPRNNG"
     gene            1343..3115
                     /locus_tag="PLV77_00745"
     CDS             1343..3115
                     /locus_tag="PLV77_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNC14408.1"
                     /translation="MDDVLLGLSLVVVLSILARLSATWMRVPPIVPLLIVGMAIGISG
                     LDLVNPSEMLGPALSPAVQIAVGIILFEGALSLKREELGSGVRSAVTRLITLGVVITW
                     FVATVSIALLLDVPHEMSILIGATVIVSGPTVVIPILNFVGPTQKVRRVLKWEGILID
                     PIGAIIAVVVFGSFDNGTGGEFDIGELFLSLGVGGLIGAAGAAVMIPILGTKRLAGRD
                     KVAATLMMVVAAFAASDAVLSDSGLVATIVMGVVLANQHRVRVDQVAEFKETLVPILV
                     GILFVLLAANVDMGQVIDLGWQGLLVIAALIVIARPLAALTLVGLPFTWREKVFFGSM
                     APRGIVAASTASAFGLELTEREVPGAEYVIPVTFLIIIGTVFYYSLLTPWLAKVLGLA
                     GEHRPTLMMIGAPPWALTIGEGLTQAGAEVLVWTESVEDAEAAARRGLVSFGGPLDPE
                     EHEADSPLEDVAAFAVVSEDDTLNQLLSFQLAEIYEANQVYRRRSPSDSSPIVRTEAL
                     ELFHDGDTETEVQKRLSAGEPVSVVGPLARVPEGAIPIAAVIPRRGTMPAKIHLATED
                     KPIKADGRSTLLVLNAVGSSPDGQ"
     gene            complement(3165..4628)
                     /gene="zwf"
                     /locus_tag="PLV77_00750"
     CDS             complement(3165..4628)
                     /gene="zwf"
                     /locus_tag="PLV77_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNC14409.1"
                     /translation="MADIGPLDVAPTALTPPEPHVVVLFGATGDLSRRKLLPGLFHLD
                     RAGHLSDCRIVGTSPEEMTNAGFRRLARDSCESFGRGRVEEDDWNRFERRLSWVSGTT
                     GAAGLSDAVDRAEADIGEQCRRLHYLSVPPAAAPAVIKTLGEAGLVERSRIIMEKPFG
                     TDLESARELNAMVHEVFDEDQVFRIDHFLGKEAALNILALRFANGLFEPIWSRNFIDH
                     VQIDVPETLTVEGRGGFYDATGAFRDMVVTHLFQVLAFVAMEPPVELAPDAITVEKNK
                     VFRSMKGIRPEDVVRGRYEGYHQESGVAEDSDAETFIALKCGIDNWRWSGVPFYLRTG
                     KAMSEGARIISIAFREPPKSMFPEGSGVGMKGPDHLTFDLDESSRISLSYYGKRPGPG
                     MLVDKLSMQFSLNEIETQGDTLEAYERLIRDAMGGDHTLFTTAEGIEELWNTARPLLE
                     NPPGVLTYERGSWGPREIHDLIEPNRWRLPFQRRWRG"
     gene            complement(4733..>5148)
                     /locus_tag="PLV77_00755"
     CDS             complement(4733..>5148)
                     /locus_tag="PLV77_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14410.1"
                     /translation="LTTDQGFERFFKPALDGQRLVNQETGSNGQYWPAGVIEVKKAGP
                     VTFTAKSAEPTAIQRLSGYSRITKLGRVAAMRGQGRWKVAMNQICGQWVDWFKRIPIS
                     IFRQNDTRQKEAARRQTLREAARFNRVEGSGAVGD"
BASE COUNT          822 a         1701 c         1657 g          968 t
ORIGIN      
        1 ggaagggtaa ttggtcgccc ggttcgtcga ggacgaggag tcagccgcgg ccgaggccgc
       61 ccggattgtc gagttggcgg ccgaggccgc gattgccgac cgggggcgct tcaatttcgc
      121 ggtcagtggc ggcaagacgc cctggcggat gcttgaactg ctggccgaaa gcgacctcaa
      181 ctggacccgg acctcgctgt tccaggttga cgagcggatt gcgccgaccg gcagcatgca
      241 gcgcaacctc acccacctgg ttctcaccct gccgctgatc tgccaggccg cgatccggcc
      301 gatgccggtc ggggccgatg acctcgcgac ggcggccagc ggttacgagt attcgctgcc
      361 ggaccgcttc gacctgatcc acctcggcat gggcacggac gggcacaccg cctcgctggt
      421 gccgggggac ccgatcctcg aggtttccga ccgccaggtt gcgatcactt ccggcgagta
      481 tcagggcacc cgtcgcatgt cgctgaccta caaggcgctt gatcgcgccc ggcagattct
      541 cttcctggtc accggggacg acaaggagga agcgctcgcg aagctggtcg actccgaccc
      601 gtcgattccg gccgggcggc tcaagaaccc caacgtcacc ctgatcaccg acatcgacat
      661 cagggattcg gaaccggttt cttgacgaag atccgcgaaa tgggggccga gcgctacggc
      721 ttcgcgctac ttctgatcct cgcctcgatg atcttcatca tggccactca tgacgggaaa
      781 tggtccaacg tcatttccct gaaccttcag gcctttgcgc tgttcgcttc aacccgggcg
      841 gcccagccga ggatggcgat tcgggtcacg gtcggtgtga tcatcggact ggtcctcgtc
      901 gccgcatgga ttcaggccgg gttcaccagt tcggtcaacg atgatttcgt gcggctttcg
      961 accttcagcc tcgtcgtgat cgcaaccccc gcgatcgcct gggggctggt gcggcaggtc
     1021 aaggctcggg gaatgatcac cacccacacg atgctcggcg tgctctgcat ctacctgctg
     1081 atgagcctcg cgttctcggc tgcgttcggg gcgatctccg cggtcagcgg cgacccgttc
     1141 ttccagcagg gcgccgccga cggcacgacc aacaactacc tctactacag cctgaccacg
     1201 atcaccacag ttggcatggg cgacttcacc ccggcgacca acctgggccg ttccctcacc
     1261 gcagccgagg cattgatcgg gcagatctac gtgatcacgg tggtcgccgt gatcgtcggc
     1321 aacctgggca gaccccgaaa caatggatga cgtcctactt ggcctgagcc tggtcgtcgt
     1381 cctctcgatc ctcgcccggc tctcggccac ctggatgcgg gtgccgccga tcgtcccgct
     1441 gctgatcgtc ggcatggcga tcggcatctc cggtctcgat ctcgtcaacc cgagcgagat
     1501 gctgggcccg gcgctctcgc ccgcggttca gattgcggtt ggcatcatcc ttttcgaagg
     1561 ggcgctcagc ctgaagaggg aggagctcgg ttccggggtg agaagcgcgg tgacccgact
     1621 gatcacgctc ggggtcgtaa tcacctggtt tgtcgccacg gtctcgatcg ccttgctgct
     1681 cgacgttccc cacgagatgt cgatcctgat cggcgcgacg gtaatcgttt ccgggccgac
     1741 cgtggtgatc ccgatcctca atttcgtcgg tcccacccag aaggttcgcc gggtcctgaa
     1801 atgggagggc atcctgatcg acccgatcgg ggcgatcatc gcggtggtcg tcttcggatc
     1861 cttcgacaac ggaaccggcg gcgagtttga catcggcgag ctctttcttt cgctcggggt
     1921 cggcggcctg atcggggcgg ccggcgccgc ggtcatgatc ccgatcctcg gaaccaagcg
     1981 gctggccgga cgggacaagg tcgcagccac cctgatgatg gtggtcgccg cctttgccgc
     2041 ctcggacgcc gtgctctccg attcgggcct ggtcgcgacg atcgtcatgg gagtcgttct
     2101 cgccaatcag catcgggtcc gggtcgatca ggtcgcggag ttcaaggaaa cgctggtgcc
     2161 gatcctggtc gggatcctct tcgtcctgct ggccgcaaac gtggacatgg gtcaggtgat
     2221 cgaccttggc tggcagggcc tgctggtcat cgccgccctg atcgtcatcg cccggccgct
     2281 ggctgcgctc actttggtcg ggctgccctt cacctggcgg gaaaaggtct tcttcgggtc
     2341 aatggctccg cgcggcatcg tcgccgcttc aaccgcctcc gccttcggcc tcgagctgac
     2401 cgagcgcgaa gtgccggggg ccgaatacgt cattccggtg accttcctga tcatcatcgg
     2461 cacggtcttc tactactcgc tgctgacccc ctggctggcc aaggtcctgg gtctcgccgg
     2521 ggagcaccgg ccgaccctga tgatgatcgg ggcaccgccg tgggcgctga ccatagggga
     2581 gggcctgacc caggccggag ccgaagtgct ggtctggacc gaatcggttg aggacgccga
     2641 ggccgcggcc cggcgcgggc tcgtttcctt cggtggaccg ctcgatcccg aggagcatga
     2701 ggcggattcc ccgctggagg atgtcgccgc tttcgccgtg gtcagcgagg atgacacgct
     2761 gaaccagctt ctgtccttcc agctggcgga aatctacgaa gccaaccagg tctaccgccg
     2821 gcgatcacct tccgacagct cgccgatcgt ccgcaccgag gcgcttgaac tgttccacga
     2881 cggcgacacc gagaccgagg tccagaagag gctttcggcc ggcgagccgg tttcggtggt
     2941 cggcccgctg gcccgggttc ccgagggtgc gattccgatc gccgccgtca tcccccgccg
     3001 cggaacgatg ccggccaaga ttcacctggc gaccgaggac aaaccgatca aggcggacgg
     3061 ccgctcaacg ctcctcgtcc ttaacgccgt cgggtcttcg cccgacggcc aatgaaaccc
     3121 gcccgcccac agagcaaaag aatggatggg cgggctccgg ttttctagcc gcgccagcgt
     3181 cgttggaagg ggaggcgcca gcggtttggt tcgatcaggt cgtggatttc ccgaggaccc
     3241 caggaaccgc gttcgtaggt cagcaccccg ggcgggttct ccagcaacgg acgggccgtg
     3301 ttccagagtt cctcgattcc ctcggcggtg gtgaagagcg tgtggtcgcc acccatcgcg
     3361 tcgcggatca gcctttcgta ggcctcgaga gtgtctccct gggtttcgat ctcgttcagc
     3421 gaaaactgca tgctcagctt gtcgaccagc atgcccggcc cgggtcgctt gccgtagtag
     3481 gagagggaga tccgcgaaga ctcgtcgagg tcgaaggtga ggtggtccgg gcccttcatc
     3541 ccgacccccg agccctccgg gaacatgctc ttgggaggtt cgcggaaggc gatagagatg
     3601 attcgtgccc cttcggacat cgccttgccg gtgcgcaggt agaagggaac cccggaccag
     3661 cgccagttgt cgatcccgca cttcagggcg atgaatgtct cggcgtcgga atcctcggcg
     3721 accccgcttt cctggtggta tccctcgtag cggccccgga ccacgtcttc cggccggatg
     3781 cccttcatcg agcgaaagac cttgttcttc tccacggtga tcgcgtccgg ggccagctcg
     3841 accggcggct ccatcgcgac gaaagcgagg acctggaaga gatgggtgac caccatgtcc
     3901 cggaaggcgc cggtcgcgtc gtagaagccg ccccgcccct cgacggtcag cgtctcgggc
     3961 acgtcgatct ggacgtggtc gatgaagttc cggctccaga tcggctcgaa gagcccgttg
     4021 gcgaagcgca gggcgaggat gttcagcgcc gcttccttgc cgagaaagtg atcgatgcgg
     4081 aagacctgat cctcatcgaa gacctcgtgg accatcgcgt tcagctcacg ggcggattcg
     4141 aggtcggtgc cgaagggctt ttccatgatg atccgcgaac gctcgaccag gccggcctcg
     4201 cccagggtct tgatcaccgc cggcgcggcg gccggtggca cgctcaggta atggagccgg
     4261 cgacattgct cgccgatgtc tgcctccgcc ctgtccaccg catcgctcag accggccgcg
     4321 ccggtggtcc cgctgaccca ggagagccgg cgttcaaaac ggttccagtc atcctcttcg
     4381 acccggcccc ggccaaagga ctcacatgaa tcccgggcga gccgccggaa cccggcattg
     4441 gtcatctcct ccggtgaggt gccgacgatc cggcaatcgg agagatgccc ggcccggtcg
     4501 agatgaaaga ggcccggcag cagcttgcgt cgggaaaggt cgccggtggc cccgaaaaga
     4561 accaccacat ggggttccgg cggggtcagc gcggtcgggg caacgtcaag gggtccgatg
     4621 tcggccatgc tcgaatctaa ccggtacccg gttgaccaat ggctaacttc ctgtcctgtt
     4681 tttcaggccg gcgctgcgac catggtccaa ccctgccgct ggccggaaga gctcagtcgc
     4741 cgaccgctcc gctgccctcg acccggttga agcgcgccgc ttcacgcagg gtctgccggc
     4801 gtgcggcttc cttctgcctg gtgtcgttct ggcggaagat ggaaatcgga atgcgcttga
     4861 accagtcaac ccactggccg cagatctggt tcatcgccac cttccagcgg ccctggccgc
     4921 gcatcgcggc gacccgaccg agtttggtga tcctcgagta gccgctgagc cgctgaattg
     4981 ccgtcggctc cgctgatttc gccgtgaagg tgaccgggcc ggccttcttc acttcgatca
     5041 ccccggccgg ccagtactgg ccgttgctgc cggtttcctg gttgaccagg cgctgaccgt
     5101 caagcgctgg cttgaagaac cgttcgaagc cctggtcggt ggtcagct
//
LOCUS       DAOQMF010000024         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_7564_length_5086_cov_3.597297, whole genome shotgun sequence.
ACCESSION   DAOQMF010000024 DAOQMF010000000
VERSION     DAOQMF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7564_length_5086_cov_3.597297"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..422)
                     /locus_tag="PLV77_00760"
     tRNA            complement(350..422)
                     /locus_tag="PLV77_00760"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(387..389),aa:Ala,seq:cgc)
     gene            533..1534
                     /locus_tag="PLV77_00765"
     CDS             533..1534
                     /locus_tag="PLV77_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14411.1"
                     /translation="MGILDEAIREHLELKRQHGADESELQGLEDEAFGEAERPDAVSP
                     SPGSEDQTQVISPGSSVTSPAGEGDQLDKVFEATRQREADAEAAVAREAAKAKGESTE
                     TPAPVDPEATFAEHPVPEPPAPAEAAGEAATVESPEAKPTEPEAAVEPPAPAQEGDAG
                     DMLEAERHHLASHPTEHYDVDAAIAEEDEIDVLSESSLSDELDRALESGETAVAGGET
                     VEYDMSSEPDSGEFEFDEPDSAEMESAEHEAEPESAGQEAVEGDSAELLYQDGATEDQ
                     PVDQDEPETGEEDAEGSSEFYDQNDVLEGTPDFLEETPEHDQLWFEQKEPKDFDFGD"
     gene            complement(1564..2418)
                     /locus_tag="PLV77_00770"
     CDS             complement(1564..2418)
                     /locus_tag="PLV77_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC14412.1"
                     /translation="MAIDDLASTLDHRDNRATGESVVRARGVNVFRWSTALRQRVMLL
                     EEIEWRVDRGQHWVMLGPNGAGKTTLLHLAAADSHPSEGTVEVLGKRLGNTDMRALRE
                     AIGLVDARTSRAIPSRRPVLETIESGAFNSIALQHQRLDASHRDRARELMAVVGLSDL
                     ATRAFGECSQGERQRILLARALMPSPELLLLDEPAAGMDLPSREQLVSALVSMAEGDP
                     GLTTVTITHHLEEIPPTATHALLIDHGRIVAQGPIDEALTSESVSAGFGLPIDVRKAS
                     GRWTAAIS"
     gene            2482..2554
                     /locus_tag="PLV77_00775"
     tRNA            2482..2554
                     /locus_tag="PLV77_00775"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2515..2517,aa:Ala,seq:tgc)
     gene            2627..5056
                     /locus_tag="PLV77_00780"
     CDS             2627..5056
                     /locus_tag="PLV77_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HNC14413.1"
                     /translation="MVDRIADPWGKRTPYGRGEAWPRRVDTFLEDRIDPEEVDEWVQT
                     ASILHSNGDGLDIAVEGGRIVGVRGREDDRVNHGRVDPKDLYGWQANNSPDRLTRPLI
                     RRDGELVECDWDTAMEALVARSTELLESGPAGWGHFGFYTSGQLFLEAYYTLGVIGKA
                     GIGTPHMDGNTRLCTATAAASLKASFGADGQPGSYTDVDHCDAIALYGHNVAETQTVL
                     WMRMLDRRAGPDPPRMLAVDPRPTPVAREADIHLAVRSGTNLALTNGLARGLIKRGWI
                     DRDYIAGHTLGFDRLEATVEPYDPKTVAGICGIEARDVEAATELIGTSERLLSTVLQG
                     FYQSNQATAAACGVNNIHLMRGMLGRPGCGLYQMNGQPTAQNTRETGADGDLPALRNW
                     ENKEHIQELADLWNVDPLKIPHWAAPTHAMKIFRYAEQGSIKLLWVSATNPAVSLPDL
                     NRVRRTLAKEDLFLVVQDIFRSETAEFADLVLPAATWGEKTGTFTSVDRTVHLSEKAV
                     EPPGEARSDLEIFLEYARRMDFRDKDGDPLIKWTDPEGAFEAWKDCSRGRPCDYSGMS
                     YERLREGPIQWPCNEANPDGTERLYTDHTFSTRPDYSETFGHDLATGASLSEEEFRAK
                     EPDGRAFLHAVPYEPSPEVTTPEHPYLLTTGRTVYHFHTRTKTGRAPQLNDAAPDVWV
                     ELHPEDAAELEIEEGDLVRIASPRGAIDAPARLNGIRRGSVFLPFHYGSWDREDEEGR
                     AANNLTVTAWDPVSKQPIFKVAAVSLQRVGAANGTPSPAPTTTASAPVAERVPATVGG
                     PSAESFCEELP"
BASE COUNT          965 a         1677 c         1669 g          775 t
ORIGIN      
        1 ggaacgactg gatcgaactc ccgatcgatc gggagttcct cgtgaccccc gtggatgcct
       61 acagcgacat tccctacggc tccaacaagt cggagaagta gaccctctct acatctagtt
      121 gttagtgcgc ggccgcctct gggcggccgc gttctattgc gaccaccccg attttcgggg
      181 cggtgaattt ggcttcccgg ctgggtttct tgcgcgacca attttgggcg agatcaaggc
      241 aaaacggggt caacttcttt gagtgagatt tcgggggttc cgaaagccac tccgcaagcc
      301 aaatattgag ttgtgagatt tttagagtca tttcttcagt gactcgtagt ggagctagcc
      361 ggagtcgaac cggcgacctc ttgggtgcga tccaagcgct ctgccaactg agctatagcc
      421 ccgcggggaa acaaggatac ggaagctcgg cgtgcaaagt agggaaagct catgcacgac
      481 gtccgcgaaa accgcataat cttaagcctt accgttagct actgagagac caatgggaat
      541 tctcgacgag gcaatccggg aacacctcga gctgaagcga cagcacgggg ccgacgaatc
      601 cgaacttcag ggactcgaag acgaggcctt cggcgaggcc gaacggcccg acgccgtaag
      661 tccctccccc gggtcggagg accagaccca ggtcatcagc cctggttcca gcgtcacctc
      721 tccggccggt gaaggcgacc agctcgacaa ggtcttcgaa gcaacccgcc agcgggaagc
      781 cgacgccgag gctgcggtcg ccagggaagc agccaaggcc aagggcgaaa gcacggaaac
      841 cccggcaccg gttgatccgg aagcgacttt cgccgagcac ccggttccgg aaccgccggc
      901 gccggccgag gcggctggcg aagcggcgac ggttgaatca cccgaggcga aaccgaccga
      961 acccgaagcg gcggtcgagc cgccggctcc cgcccaggag ggcgatgccg gggacatgct
     1021 cgaggccgaa cgccaccacc ttgccagcca tccgaccgag cattacgacg tcgatgccgc
     1081 gatcgccgag gaggacgaga tcgacgtgct cagcgagagt tcactctcgg atgaactcga
     1141 ccgggccctg gaaagcggcg aaaccgccgt ggccgggggc gagaccgtcg agtacgacat
     1201 gtcatcggaa ccggactcgg gcgagttcga gttcgatgaa cccgactcgg ccgaaatgga
     1261 atcagccgaa catgaagcgg agccggaatc ggccgggcag gaagcggtcg aaggggattc
     1321 ggccgagctg ctctaccagg atggcgccac cgaggatcag ccggtcgatc aggacgagcc
     1381 ggaaaccggt gaagaagacg ccgagggttc gagcgagttc tacgatcaga acgatgtgct
     1441 cgagggcacc ccggactttc tcgaagaaac gcccgagcac gaccagctct ggttcgagca
     1501 gaaggaaccg aaggacttcg acttcggcga ctgagctggt tctgccggtc gccgtcgaac
     1561 cagtcagctt atggccgccg tccagcggcc tgacgctttg cgaacatcga tcggcaggcc
     1621 gaaaccggca gacactgatt cagaggtgag tgcctcgtcg atcgggccct gggcgacgat
     1681 ccggccgtga tcgatcagca gtgcgtgggt ggcggtcggc gggatctctt ccaggtggtg
     1741 ggtgatggtg accgtggtca ggccggggtc tccttcagcc atcgagacca gcgcggaaac
     1801 gagctgctcg cgcgagggca gatccatgcc ggcagccggc tcgtcgagga gcagcagctc
     1861 agggctcggc attagcgccc gggcgagcag gatccgctgc cgctccccct gcgagcactc
     1921 gccgaaggcc cgggtggcga ggtcgctcaa accgacgacg gccatcaatt cccgggcccg
     1981 gtcgcggtgg gaagcgtcca ggcgctggtg ctggagagcg atgctgttga aagctccgga
     2041 ctcgatcgtc tcgaggaccg ggcggcggga ggggatcgcc cgcgaggtgc gggcgtcaac
     2101 cagtccgatg gcttccctca gcgcccgcat gtcggtgttg cccagccgct tgccgagcac
     2161 ttcgaccgta ccttcggaag ggtgggaatc ggcggcggcc aggtggagga gggtcgtctt
     2221 gccggcgccg ttcgggccga gcatcaccca gtgctggccg cggtccacgc gccactcgat
     2281 ttcctccagc agcatcaccc gctgtcgaag tgcggttgac cagcgaaaga cgttcacccc
     2341 cctggcccgg accacggatt cgcccgttgc ccggttgtcc cggtgatcga gggttgaggc
     2401 aaggtcgtcg atcgccaccg gctcaggcta tctgcggcag ggtccggccg cccccgaacc
     2461 cgtacaatcg ccgtccactc tggggccata gctcagttgg cagagcgcct gctttgcaag
     2521 caggaggtcg ccggttcgat cccggctggc tccactcgcg tgaccggtaa cgggttgcgg
     2581 aaggtcggcg tgattggcgc gctgtcgcgt gggtacctga tctctcatgg tcgaccggat
     2641 cgcggatccc tgggggaaaa ggactcccta cggaaggggg gaggcgtggc cgcgccgggt
     2701 ggacaccttt ctcgaggacc ggatcgaccc ggaagaagtg gacgaatggg tgcagaccgc
     2761 ctcgatcctt cactcgaatg gtgacgggct cgatatcgcg gtcgagggcg ggcgaatcgt
     2821 cggagtccgc ggacgcgagg acgaccgggt caaccacggc cgggtcgatc cgaaagacct
     2881 gtacggctgg caggccaaca acagtcccga ccggctgaca cggccgctga tccgccggga
     2941 cggtgaactc gtcgaatgcg actgggacac cgccatggaa gccctggtcg cccgctcgac
     3001 cgagttgctg gaatccggac ccgccggctg gggccacttc gggttctaca cgtccggcca
     3061 gctcttcctt gaggcctatt acacgctcgg cgtgatcggc aaggccggta tcggcactcc
     3121 gcacatggac ggcaacaccc gcctgtgcac cgccaccgca gcggcctcgc tcaaggcgag
     3181 cttcggcgcc gacgggcagc ccggttccta cacggacgtc gaccactgcg acgccatagc
     3241 cctctacgga cacaacgtgg ccgagaccca gaccgttctc tggatgcgaa tgctggaccg
     3301 ccgggccggg ccggacccgc ctcggatgct ggccgtggac ccgcgaccga cccccgttgc
     3361 ccgcgaggcg gacatccacc tggcggtgag gagcggtacc aacctggccc tgaccaacgg
     3421 cctggcccgc gggctgatca aacggggatg gatcgaccgg gattacatcg ccgggcacac
     3481 gctgggcttc gaccgtctcg aagcgacagt ggagccttac gacccgaaga cggtggccgg
     3541 catctgcgga atcgaagccc gcgatgtcga ggcggcgacc gaactgatcg gcacttccga
     3601 gcgactgctc tccactgtcc ttcagggctt ctaccagtcg aaccaggcga ccgcggccgc
     3661 ctgcggggtc aacaacatcc acctgatgcg cgggatgctc ggccggccgg gatgtggcct
     3721 ctaccagatg aacggacagc cgaccgcgca gaacacgcgc gagaccggcg ccgacggcga
     3781 cctgcccgcc ctgcgcaact gggaaaacaa ggaacacatc caggaactgg ccgacctctg
     3841 gaacgtggac ccgctgaaga tcccgcactg ggcggcaccg acccacgcga tgaagatctt
     3901 tcgctacgcg gagcagggct cgatcaagct gctctgggtc agcgccacca atcccgcggt
     3961 ttccctgccc gacctgaacc gggtgcgccg gacccttgcg aaggaggatc tgttcctggt
     4021 ggtccaggac atcttccgga gcgaaaccgc cgagttcgcc gatctggtcc tgcccgccgc
     4081 tacctggggc gagaagaccg gcaccttcac cagcgtcgac cggaccgtcc acctgtccga
     4141 gaaggcggtc gaaccgccgg gcgaggcgag gtcggacctc gagatcttcc tcgaatacgc
     4201 acggcggatg gacttccggg acaaggacgg cgatccgctg atcaagtgga ccgacccgga
     4261 aggcgccttc gaagcctgga aggactgttc gcgcggacgg ccctgcgact actcgggaat
     4321 gagctacgaa aggctgcgcg agggtccgat ccagtggccc tgcaacgaag ccaacccgga
     4381 cggcaccgaa cgcctctaca ccgaccacac cttttcgacc cggcccgatt actcggaaac
     4441 cttcggccac gaccttgcga ccggtgccag cctgagtgaa gaggagttcc gggcgaagga
     4501 gccggacgga cgcgccttcc ttcacgccgt cccctacgag ccttcgcccg aagtcaccac
     4561 tccggagcac ccgtacctgc tcaccaccgg tcgaaccgtc taccacttcc acacccggac
     4621 caagaccggg cgcgcaccgc aactgaacga cgccgcgcct gatgtctggg tcgagcttca
     4681 ccccgaagat gcggcggaac tcgagattga agaaggcgac ctggtccgca tcgcctcgcc
     4741 gcggggcgcg atcgacgcgc cggcccggct caacgggatc cgccggggtt cggtcttttt
     4801 gccgttccat tacggaagct gggaccgcga agacgaggag ggccgggccg ccaacaacct
     4861 caccgtcacc gcctgggacc ccgtttccaa acagccgatc ttcaaggtcg ccgcggtcag
     4921 ccttcagcgg gtcggtgccg ccaacgggac cccgtccccg gcgccgacca ctaccgcctc
     4981 ggctccggtc gccgagaggg tgccggcgac ggtcggcggg ccttcggccg aatcgttttg
     5041 cgaggagctc ccgtgaacct cggccactac ctctccctga tcagcc
//
LOCUS       DAOQMF010000025         5039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_7686_length_5039_cov_3.699438, whole genome shotgun sequence.
ACCESSION   DAOQMF010000025 DAOQMF010000000
VERSION     DAOQMF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7686_length_5039_cov_3.699438"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PLV77_00785"
     CDS             complement(<1..1822)
                     /locus_tag="PLV77_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14414.1"
                     /translation="MGITNARRFVALAFALVMAFALTAAFANSQAEAGKKKKKAKPAT
                     VKVVNITTANQQALLKQKKLTVKVKSTGKASPKVSAVSSGKSNYFKSVTVKFKKKGTK
                     TVSLALTSAGKTALGTCGAKTVQVNASYKKGKKKAKASKKKTLGKWADNSNCFVTVDV
                     GDDPAHCDFLDATQCLQPWPNDYYTKEDSSTPTGKRLNLGAESTPMNTGNATPVHLDV
                     TDINRGDGFSPGNEILIKIPGLDTPAAFTNSNLVPLDDISAYKDAAQAVIVIDAATGE
                     RQPIWAELDSNPTSVNPTEEDPGGININPTNTQPVNLIVHPAKNFEFGHRYIVAFRNL
                     KDADNNAIQSPLGFRVYRDNLKTKQSVVENRRSHMNSVISDLVNKAGVDRSSLYMAWD
                     FTVASEDSVTGRATTIRDDAFARLGDNNLADRQIAGNSPDIDVLAYCDKSNVAAPSCG
                     NNYPGIPEFNGSNPVTSPVPGTDIQRTVVGYIRDVPCYLDQDGCPASGSQFAFDSNGN
                     LTWNNTFKMDVPFRCMIPSSIVNTGTVVPGHTGTYGHGLLGSLNQINATVEAGNRTNT
                     VFCAANWDGFSTLDAGVVIQSLMNMSNFNKLVDRMQQGFVN"
     gene            complement(2010..3074)
                     /locus_tag="PLV77_00790"
     CDS             complement(2010..3074)
                     /locus_tag="PLV77_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNC14415.1"
                     /translation="MTTTPALLDELLRTPGPSGYEAPAADVWRKAAGFAEITTDGLGS
                     SAATIPSSDENDEAPLVAVVGHIDEIGLVITHIDEKGFLYFAPIGGWDPQILVGQRVT
                     ITGNEGPVPGVAGRKPIHLLEPDQRKKVVQLKEMHIDIGADDREDAQKMVSVGDPVVI
                     DCEPQAIAGGRLVSRSMDNRLGSYVALEAARRIHENARAKVRFAGVAAVQEEIGLFGS
                     RTAAFTFRPDLAVAIDVTHATDAPGIDEKEVGSHPFGSGPVIGRGSTLSPKVYELLRD
                     AAEKAGIDYSVGASGNSTHTDADSLQISRTGIPTGLVSIPLRYMHSPVEMVDLADVEA
                     TVALMVAFVEGLAVDVDLSR"
     gene            3134..3694
                     /gene="efp"
                     /locus_tag="PLV77_00795"
     CDS             3134..3694
                     /gene="efp"
                     /locus_tag="PLV77_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNC14416.1"
                     /translation="MISTSDFRNGSHIEVEGKIMRIIEFQHVKPGKGGAFVRTKLRRI
                     EDGGVIDKTFRAGEKFRPVRTESKRMQYLYESGDAVVFMDNQSYDQIEIPADIAADAM
                     RWIQPNDEVEVLFVDENPSDVSVGSSIEAKVVKTEPGLKGDTASGGGTKPATLESGVE
                     VQVPLFINEGEVIKVNTETAEYMSRA"
     gene            3915..4349
                     /locus_tag="PLV77_00800"
     CDS             3915..4349
                     /locus_tag="PLV77_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNC14417.1"
                     /translation="MEMFQRDLTLEARSRGFHLVTDEIERGLPELGTLRIGLIHLLIR
                     HTSASLTLNENASPDVRRDFRSWIDAVVPERPTAYGWTHTLEGADDMPAHIKGSVTGF
                     DLTIPVRDGCFHLGAWQGIYLCEHRDHGGARSLTATGWGEIG"
     gene            complement(4298..>5039)
                     /locus_tag="PLV77_00805"
     CDS             complement(4298..>5039)
                     /locus_tag="PLV77_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14418.1"
                     /translation="GLHGLLPPNGILEVIRSSSPDPHQPPPAHASRAIHPGLVIHRTR
                     SLKRGEVRNRHGIPVTSAVRTVINLAEREPPRSLDTAIRRGIARNLFDLDELNRAIDR
                     ARGRKGIAKLRKVLASWNPGKLRTKSGLENKTADRFRAWGIPDPLINDFRCGHEVDFQ
                     WPGSNILVESDGGAYHASRADRHRDYQKMLDLQAAGNVVVRIDEDMVEKEAYEVGLKL
                     RKLFAEEGVVELQPISPQPVAVSDRAPP"
BASE COUNT          861 a         1514 c         1646 g         1018 t
ORIGIN      
        1 aattcacgaa gccctgctgc atgcggtcga ccagcttgtt gaagttgctc atgttcatga
       61 gcgactgaat gaccacgccg gcgtccagcg tcgagaagcc atcccagttg gcagcgcaga
      121 aaaccgtgtt ggtccggttt ccggcttcaa ctgtcgcgtt gatctggttc agcgatccga
      181 gcagaccgtg accgtaggtg ccggtgtgac ccggaaccac ggtgcccgtg ttcacgatcg
      241 acgacgggat catgcaccgg aagggaacgt ccatcttgaa ggtgttgttc caggtcaggt
      301 tgccattcga gtcgaaggcg aactgcgacc cgctcgcggg gcagccatcc tgatccaggt
      361 agcagggcac gtcgcggatg tacccgacga ccgtgcgctg gatgtcggtg cccggtaccg
      421 gcgaggtaac cggattcgac ccgttgaact cggggatgcc ggggtagttg ttgccacagc
      481 tcggagccgc gacgttgctc ttgtcgcagt aagcgaggac gtcgatgtcc gggctgttgc
      541 cggcgatctg gcgatcggcg agattgttgt cgccgagacg cgcgaaggcg tcgtcacgga
      601 tcgtcgtggc gcggccggtc acgctgtcct cggaggcaac ggtgaagtcc catgccatgt
      661 agaggctgga acgatcaacg ccggccttgt tgaccaggtc gctgatgacc gagttcatgt
      721 gggagcgacg gttctcgacg acgctctgct tggtcttcag gttgtcccgg tatacccgga
      781 atccgagcgg cgactggatc gcgttgttgt cggcgtcctt caggtttcgg aaggcgacga
      841 tgtaccggtg gccgaactcg aagttcttgg ccggatggac gatcagattg accggctggg
      901 tgttggtcgg gttgatgttg attccgcctg gatcctcttc ggtcgggttg accgaggtcg
      961 ggttcgagtc gagctcggcc cagatcggct gacgctcgcc ggtggcggcg tcgatcacga
     1021 tcacggcctg ggccgcatcc ttgtaggcgc tgatgtcatc aagcggaacc aggttgctgt
     1081 tggtgaaagc agccggagtg tcgaggcccg gaatcttgat caggatctcg ttgccggggc
     1141 tgaagccgtc accgcggttg atgtcggtga catcgagatg gaccggggtg gcgttgccgg
     1201 tattcatcgg cgttgactcg gcgccgaggt tcaggcgctt gccggtcggg gtcgacgaat
     1261 cttccttggt gtagtaatcg ttcggccagg gctggaggca ctgggtcgca tcgaggaagt
     1321 cacagtgcgc gggatcgtcg ccaacgtcaa ccgtcacgaa gcagttgctg ttgtcagccc
     1381 acttgccgag ggtcttcttc ttcgaagcct tggccttttt cttgcccttc ttgtatgagg
     1441 cgttgacctg gacagtcttg gcgccacagg tgccgagggc ggtcttgccg gcagaagtca
     1501 gggcgagact cacggtcttg gtgcccttct tcttgaactt gacggtgacc gacttgaagt
     1561 agttgctctt tccggagctc acagcggaaa ccttcggtga agccttgccg gtcgacttga
     1621 ccttcaccgt cagcttcttc tgcttgagca gggcctgctg gttcgcggtc gtgatgttga
     1681 cgaccttgac ggtggccggc ttggcctttt tcttcttctt tcctgcctcg gcctggctgt
     1741 tggcaaatgc tgccgtcagc gcgaaggcca tgaccagggc gaacgcgagc gccacgaaac
     1801 ggcgtgcatt cgttattccc aattggtgat tcatctctcc gggtaactcc ctctcatagc
     1861 tttgaccggc tgtaacaccg gccgcgattt gtggccgacc ggaaacaccg gtcgtgtgac
     1921 ttgactcaca gatactagac cggattcatg gcggaagttg cgccgatcgg acaggatgtt
     1981 tctgaacgat tccgtaccga aagaagggtt cagcgcgaga ggtccacatc gaccgcgagg
     2041 ccttcgacga aggcgaccat gagcgccacc gttgcctcga cgtcggccag gtcgaccatc
     2101 tcgaccggcg aatgcatgta gcgcagcggg atcgagacga gccccgtcgg gatgccggtc
     2161 cgggagatct gaagggaatc ggcatcggta tgggtcgagt ttccactggc gccaaccgag
     2221 taatcgatgc cagccttttc cgcggcgtcg cggagcagtt cgtagacctt gggggagagg
     2281 gtcgaacccc gtccgatcac cggtcccgac ccgaacgggt gactgccgac ttccttttcg
     2341 tcgatgcctg gagcgtcggt cgcgtgggtg acgtcgatcg ctacggcgag atcgggtcga
     2401 aaggtgaagg cggcggtgcg ggacccgaac aggccgattt cctcctgaac cgcggcgacg
     2461 ccggcgaatc gaacctttgc cctggcgttc tcgtggatcc gccgggccgc ctcgagcgcc
     2521 acataggacc ccagccggtt gtccatcgag cgggaaacga ggcgtcctcc ggcgatcgcc
     2581 tgcggttcgc agtcgatcac gaccgggtct ccgacgctga ccatcttctg cgcgtcctcg
     2641 cggtcatcgg cgccgatgtc gatgtgcatc tccttcagct ggacgacctt cttgcgctga
     2701 tccggctcga gcaggtggat cggcttgcgc ccggcgacgc cgggcacggg accttcgttg
     2761 ccggtgatcg tgacgcgctg cccgaccagg atctggggat cccagccgcc gatcggcgcg
     2821 aagtaaagga agcccttttc gtcgatgtgg gtgatgacca ggccgatctc gtcgatgtgg
     2881 ccgaccaccg cgacgagggg cgcttcgtca ttttcgtccg aactcgggat tgtcgccgcg
     2941 ctggaaccga gaccgtcggt ggtgatctcg gcgaaaccgg ccgccttgcg ccagacgtcg
     3001 gcggcgggag cttcgtagcc ggaagggccg ggggtgcgga gcaattcgtc gagaagcgcg
     3061 ggagtcgtag tcatggattg attatgccgt tcggcgcgac tcgcccgggc gccctgtggc
     3121 taaagtgtct gccgtgattt caaccagcga tttccgcaac gggtcccaca tcgaggtcga
     3181 aggcaagatc atgcgaatca tcgagttcca gcatgtgaag cccggcaagg gcggtgcgtt
     3241 cgtgcgcacc aagcttcgcc gcatcgaaga cggcggagtc atcgacaaga ctttccgcgc
     3301 cggggagaag ttccggccgg ttcgcaccga gtcaaagcgg atgcagtacc tctacgagtc
     3361 tggtgacgcg gtcgtcttca tggacaacca gagctacgac cagatcgaaa ttccggccga
     3421 tatcgctgcg gacgcgatgc gctggatcca gcccaacgat gaagtcgaag tcctattcgt
     3481 ggacgagaac ccctccgatg tctcggtcgg cagctcgatc gaagcgaaag tggtgaagac
     3541 cgaacccggc ctgaagggcg acaccgcctc gggcggcggc acgaagccgg cgacccttga
     3601 gtcgggagtc gaggtccagg tgccgctttt catcaacgag ggcgaagtga tcaaggtcaa
     3661 caccgagact gccgaataca tgtcccgcgc ctgaaccccg gccacccccg gacccggaca
     3721 cccccgaacc cggcccaggc ccccaacccg ggccccagcc caaagcctgc ccaccgggtg
     3781 ggtcggattt ccgtcatcag gacctgaatc ccgacccacc aggcaccgcc gcttgaacct
     3841 cccgcagggg tgggtcggat ttccgtcctg gcagctggtt atccgaccca cctccgggcg
     3901 ggtacggttc tgcgatggag atgtttcagc gagatctgac tctcgaggct cgatccaggg
     3961 gctttcacct ggtcacggac gagatcgaac ggggcctccc ggagctcgga acactgcgga
     4021 tcggcctgat ccacctgctg atccggcata cgtcggcttc attgaccctg aacgaaaacg
     4081 cctcgcccga tgtgaggcgg gacttcaggt catggatcga cgcggtcgtg ccggaacggc
     4141 cgaccgccta cggctggacg cacacccttg agggcgccga cgacatgccg gctcacatca
     4201 agggttcagt gaccggattc gacttgacga ttccggtgcg ggacgggtgc tttcatcttg
     4261 gagcctggca ggggatctac ctctgcgaac accgggatca cggtggcgcc cggtcgctga
     4321 ccgcgaccgg ctggggtgaa atcggctgaa gctcgaccac gccttcttcc gcgaagagct
     4381 ttcgcagttt caaccccact tcgtaggcct ccttttcgac catgtcctca tcgatccgca
     4441 cgacgacgtt tcctgccgcc tggagatcga gcatcttctg atagtcccga tgccggtccg
     4501 cccgactcgc gtgataggcg ccaccgtcgg attcgaccag gatgttcgat cccggccact
     4561 gaaagtcgac ttcgtgaccg cagcggaagt cgttgatgag tgggtccggg atgccccacg
     4621 cgcggaaccg gtcagccgtc ttgttctcca ggcccgattt ggtcctgagc ttccccgggt
     4681 tccagctcgc gagcaccttc cttagtttcg cgatgccttt ccgcccccgg gcccggtcaa
     4741 tggcccggtt caattcgtca aggtcaaaga ggtttctggc aatgcctctc ctgatggcgg
     4801 tgtcgagcga cctgggcggt tccctttcag ccagattgat cacggttcga accgctgatg
     4861 tgaccggaat gccgtggcga ttcctcactt cgccgcgctt gagggaccgg gtccggtgga
     4921 tgaccagacc ggggtggatt gcccggcttg cgtgcgcggg cggcggctgg tgagggtcgg
     4981 ggcttgagct gcggatcact tcgaggatcc cgttcggcgg cagcaggccg tgaaggccg
//
LOCUS       DAOQMF010000026         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_7980_length_4922_cov_3.327923, whole genome shotgun sequence.
ACCESSION   DAOQMF010000026 DAOQMF010000000
VERSION     DAOQMF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7980_length_4922_cov_3.327923"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="PLV77_00810"
     CDS             <1..1747
                     /locus_tag="PLV77_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC14419.1"
                     /translation="YAKARFMRSMTDILIIAPPVILIAFFFFSDAKELTLSTTEVDAK
                     TVNASNPHPVVMVIFDEFPAGSLMTPSGRINPKRFPNFAGLKGTSTWYRNTATDASYT
                     AIAVPSILTGQPADHDSLPTAADHPDSIFTLLGGSWQTHAIEPITQLCTEEICGEREG
                     GKTGMADALSSLTTDLKAVSAHLLLPADMGSSLPDISQSFQGFGGEEDSVERGRARQW
                     VHDRLGEGENSLDGEADVDAFIRRLGSGGKSFDFVHVEKPHYPWTHYPSGLKYSDGTE
                     DFRSFIGETDWEGDGYITDRAHQAHLLEVGFTDNLLGRIISALKRDGRWDDTLFVATA
                     DHGAAWTKGAGRRSAEEETMGQIGMVPLFIKAPGQEQGKVVDRPTCTTEIVPRMAQIL
                     GIDLPWTPATCDRKTVRIDNGSGKPVTLAFVKAIAQRQLYINRMARLLGGDGADWDRA
                     FRWGKRSDLIGQSTARFWRIGATNDAEASSETPSPRSFTFDSGIELNPVLRQRGTISG
                     LDEGTPLAVGVNGRIAAVGQSYEDNGQVRYSILLPQSTLREGSNRIQLFEVAPFTGGA
                     AEAAPLRLLWSSTDQ"
     gene            1760..4132
                     /locus_tag="PLV77_00815"
     CDS             1760..4132
                     /locus_tag="PLV77_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC14420.1"
                     /translation="MTDVAKSGRESGNGGKAPFKRPSLILGAAHLAALWALTFAQPML
                     DLLGENPDFFVARGNSSGQIILYALVLTFAVPLVCLAIEALVRLASRDAQWYLHLFLV
                     GLFGACFVLQLIKNHLDWPAGILIAISIGLAVAGVFIYSRWRFPQAFMDVLSVAPIII
                     LVIFFGFSSTSRLVLPREQPDPVDVKIGTPAPVVMVVFDEFPVGSLMKPDGQIDGTRY
                     PAFAELAGHSTWYRNTSAAAAYTPLAVPAIFSGQKPNNDDLPIASDYPHSVFTLLGKT
                     YDLRVMEAATRICPDTLCPEEDNTAQDGSTSDLFSDLYVVSKYLLLPDSLTRNLPDVS
                     NSFSGFTESDQVDTNAESEVTGLDDGGETGATGTTGLTGATGATGDTQPERTGQGAAR
                     KLGRLFALESSADEFERVGEFNAKLKRGQQETLDLIHIEKPHYPWRHIPNGQRYSNLT
                     AEWSGLLPNDGPWMAPPRIVNIAMQRHLLEVGYTDTLLAQIIKRLKQTGLWERAMVVV
                     TADHGAAFQSKVPRRAATSENMGEVASVPLFIKSPGQVKPEVTDRRTCATDILPMIAD
                     QLGIEYPWPVAECDPERVTVVNSPNGEGFTSPAKMLRQRQKLLINRIQSVFGTGHGWG
                     PYYRFGPHRELIGKKVKNLEVLPLKRWRALPDERNAAKNYDPTAPTLRGLLQRGVTDR
                     IPEGSVLAVAVDGKIQAVGWTFKDGTGRGPGFSILLPPESLRAGFNRIAIYLLRDNGK
                     KLRLVYDGSRPLAPELVEAQNEARAEYRREMGLPALPDPLSTGGQNGDGS"
     gene            4122..4487
                     /locus_tag="PLV77_00820"
     CDS             4122..4487
                     /locus_tag="PLV77_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC14421.1"
                     /translation="MGPETPLRIAGPADIGPVTRLIAGFRDFLADDSPTNAEIEATVG
                     VLLRDRSTEFLLVGDPEAGFVQTRFRLSVWTGNEDAWLEDLFVDESARGKGYGRTLVE
                     AAVERARSRGCDRIQLDVN"
     gene            complement(4563..>4922)
                     /locus_tag="PLV77_00825"
     CDS             complement(4563..>4922)
                     /locus_tag="PLV77_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14422.1"
                     /translation="GELRTLSPSAGKPGFYDTLDGGRPPLLGPAIVVNRVVGAVGRGE
                     LRAALVPEGMEFLAENHVNVIRSRGPAVDWEGVLAALRMPGVVERVRLLTGNTQISAR
                     ELTHLLPLDLAGVEEVG"
BASE COUNT          861 a         1721 c         1546 g          794 t
ORIGIN      
        1 ctacgccaag gcccgcttca tgcgctcgat gaccgacatc ctgatcatcg ccccgccggt
       61 gatcctgatc gccttctttt tcttcagcga cgccaaagag ctcacgctct cgaccacgga
      121 ggttgacgcc aagacggtga acgcctcgaa cccgcatccg gtcgtgatgg tcatcttcga
      181 cgagttcccg gccggctcgc tgatgacccc ttcgggccgg atcaacccga agcgcttccc
      241 caacttcgcc ggcctgaagg gcacctctac ctggtaccgg aacacggcga ccgacgcttc
      301 gtacacggcg atcgcggtgc cctcgatcct gaccggccag cccgcggatc acgacagcct
      361 gccgaccgcg gccgaccacc ccgattcgat cttcaccctg ctgggcggga gctggcagac
      421 ccacgcgatc gaaccgatca cccagctctg caccgaggag atctgcggcg agcgggaagg
      481 cgggaagacc ggcatggccg atgcgctctc ctccctgacc actgacctga aagcggtttc
      541 cgcccatctg ctgctgccgg ccgacatggg ttcctccctg ccggacattt cccagagctt
      601 ccagggtttc ggtggcgagg aggactcggt agagcgtggc cgggcccgcc agtgggtgca
      661 tgaccgcctc ggcgagggcg agaactcgct ggacggtgaa gctgacgttg acgcgttcat
      721 ccgccggctg ggaagcggcg gcaagagctt cgacttcgtt cacgtcgaga agccgcacta
      781 tccctggacc cactacccct cggggctgaa gtactccgac gggaccgagg acttccgcag
      841 cttcatcggt gaaaccgact gggagggtga cggctacatc acggaccggg cccatcaggc
      901 ccatctgctc gaggtcggct tcaccgacaa cctgctcggc cggatcattt ccgcgctgaa
      961 gagggacggc cgctgggatg acacgctgtt cgttgccacg gccgatcacg gtgcagcctg
     1021 gaccaaaggt gccggccgac gctcggcgga ggaagaaacg atggggcaga tcgggatggt
     1081 cccgctcttc atcaaggcac ccggccagga gcagggcaag gtagtcgacc gaccgacctg
     1141 caccacggaa atcgtccccc ggatggcgca gatcctcggc atcgacctgc cctggacgcc
     1201 ggccacctgc gaccgcaaga ccgtccggat cgacaacgga agcggcaagc cggtgactct
     1261 cgccttcgtc aaggcgatcg cccagcgcca gctctacatc aaccggatgg cccggctgct
     1321 cggcggcgac ggagccgact gggaccgcgc cttcaggtgg ggcaaacgga gcgacctgat
     1381 cggccagtcc acggcccggt tctggaggat cggcgccacg aacgacgccg aagcgagctc
     1441 cgaaactccc tcgccccgct cgtttacgtt cgattcgggg atcgaactga acccggtcct
     1501 gaggcaacga ggcacgattt ccggactgga cgaaggcacg ccgctggcgg ttggcgtcaa
     1561 cggaaggatc gccgcggtcg gccagtccta cgaagacaac ggccaggtca ggtactcgat
     1621 cctgctgccg caatcaacgc tccgggaagg gtccaaccgg attcagttgt tcgaggtggc
     1681 tccattcacg ggaggcgccg ccgaagcagc ccccttgagg cttctctggt cctcgaccga
     1741 ccaatagact ggccagtccg tgactgatgt cgccaagtcc gggcgtgagt ccggaaacgg
     1801 aggtaaagcg ccgttcaagc gtccgtccct gattctgggg gcggcccatc tggctgcgct
     1861 ctgggcgctc accttcgccc agccgatgct cgacctgctc ggcgagaacc cggacttctt
     1921 cgtcgcccgg ggcaacagct ccggccagat catcctttac gccctggtcc tcacctttgc
     1981 ggtgccgctg gtttgcctcg cgatcgaggc gctggtccgc ctggccagtc gcgacgccca
     2041 gtggtatctc caccttttcc tggtcggact gttcggggcc tgcttcgtcc tgcagctgat
     2101 caagaaccac ctcgactggc cggccgggat cctgatcgcg atttcgatcg gcctggccgt
     2161 ggccggggtc ttcatctatt cccgctggcg ctttccccag gccttcatgg acgtgctcag
     2221 tgtcgccccg atcatcatcc tggtgatctt cttcggtttc agcagcacct cgcgcctggt
     2281 cctgccccgg gagcagccgg accccgttga cgtgaagatc ggaactccgg ccccggtcgt
     2341 gatggtcgtc ttcgacgagt tcccggtcgg ctcgctgatg aagcccgacg gtcagatcga
     2401 cggaaccaga tacccggcct tcgccgagct ggccggccat tcgacctggt accggaacac
     2461 cagcgccgcc gctgcctaca ccccgcttgc cgtgccggcg atcttctccg ggcagaagcc
     2521 gaacaacgac gacctgccga tcgcctccga ctatccgcac agcgtcttca ccctgctcgg
     2581 caagacctat gacctgagag tgatggaggc cgccacgcgg atctgccccg acacgctctg
     2641 tccggaagag gacaacaccg cgcaggacgg ctcgacctcc gacctcttct cggaccttta
     2701 cgtcgtctcc aagtacctgc tgctgccgga ttcgctgacc cggaacctcc ccgacgtctc
     2761 caactccttc tcgggcttca ccgagtcgga tcaggtcgac accaacgctg aatccgaggt
     2821 gaccggtctc gacgacggcg gtgaaaccgg ggcaaccggc accaccggac tgaccggagc
     2881 gacgggcgct accggagaca cccagccgga gcggaccggg cagggtgccg cccgcaagct
     2941 cggccggctg ttcgcgctcg aatcctcggc cgacgaattc gagcgggtcg gcgagttcaa
     3001 cgcgaagttg aagcggggcc agcaggagac cctcgacctg attcacatcg aaaagcccca
     3061 ctacccctgg cgccacatcc cgaacggcca gcgctactcg aacctgaccg cggaatggtc
     3121 gggcctgctg cccaatgacg gtccctggat ggccccgccg cggatcgtca acatcgccat
     3181 gcagcggcac ctgctcgagg tcggatacac cgacaccctg ctcgcccaga tcatcaagcg
     3241 gctgaagcag accgggctct gggagagagc catggtcgtc gtcaccgccg accatggagc
     3301 cgccttccag tcgaaggtcc cgcgccgggc ggcgaccagc gagaacatgg gggaggtcgc
     3361 gtcggtcccg ctcttcatca agtcacccgg gcaggtcaaa ccggaagtga ccgaccggcg
     3421 cacctgcgcg accgacatcc tgccgatgat cgccgaccag ctcgggatcg agtatccgtg
     3481 gcccgtcgcc gaatgtgacc cggagcgggt caccgtggtc aattcgccga acggggaggg
     3541 tttcacctcc cccgcgaaga tgctgcggca gcggcagaaa ctgctgatca accggatcca
     3601 gagcgtcttc gggaccggtc acggctgggg cccctactac cgcttcgggc cccacaggga
     3661 actgatcggc aagaaggtga agaacctcga ggtgctgcca ctgaagcgct ggcgcgccct
     3721 gccggatgaa cggaacgcag ccaagaacta cgacccgacc gcccccaccc tgcgcggcct
     3781 gctccagcgc ggagtcaccg accggatccc ggaagggagt gtgctggccg tggccgtgga
     3841 cgggaagatc caggcggtcg gctggacctt caaggacggc accggccgcg ggcccgggtt
     3901 ctcgatcctg cttcctcccg agtcgctcag ggccggcttc aaccggatcg ccatctacct
     3961 gctccgggac aacgggaaaa agctccgcct ggtctatgac ggttccaggc cgctggcacc
     4021 cgagctggtt gaagcgcaga acgaggctcg ggccgagtac cgcagggaga tgggactgcc
     4081 cgccctgccg gacccgcttt cgaccggagg ccagaacggt gatgggtcct gagaccccgc
     4141 tcagaatcgc cgggccggcc gatatcggac cggtcacccg cctgatcgcc ggtttccggg
     4201 atttcctcgc ggacgactcg ccgaccaacg ccgagatcga agcgacggtc ggggtccttt
     4261 tgcgggaccg ctcgaccgag ttcttgctgg tcggcgaccc cgaggccggg ttcgtgcaga
     4321 cccgtttccg gctttcggtc tggaccggca acgaggacgc ctggctggaa gacctcttcg
     4381 ttgacgagtc ggctcgcggg aagggttacg ggcgcaccct ggtcgaggcg gcggtcgagc
     4441 gggccaggtc ccgcggttgc gaccggatcc agctcgatgt caactaggcc aacgagccgg
     4501 cggtgaagct ctacgaatcc tgcgggttcc ggccagtcca caatccggag aagtggggaa
     4561 gttcacccga cttcttctac acccgcgaga tccagcggca gcaggtgggt tagctcccgc
     4621 gccgaaatct gggtgttccc agtcagcagc cggacccttt cgaccactcc cggcatccgc
     4681 aacgcggcga ggacgccttc ccagtcaacc gccgggccac ggctgcggat cacgttgacg
     4741 tggttctccg ccaggaactc catcccctcc ggaacgagcg ccgcccgcag ctccccccgg
     4801 ccgaccgccc cgaccacccg gttgaccacg atcgccgggc cgagaagcgg cggtcggcca
     4861 ccatcgagcg tgtcgtagaa gcccggtttc ccggcgctcg gggagagggt ccggagttcc
     4921 cc
//
LOCUS       DAOQMF010000027         4884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8085_length_4884_cov_4.153655, whole genome shotgun sequence.
ACCESSION   DAOQMF010000027 DAOQMF010000000
VERSION     DAOQMF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8085_length_4884_cov_4.153655"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..320
                     /locus_tag="PLV77_00830"
     CDS             15..320
                     /locus_tag="PLV77_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNC14423.1"
                     /translation="MNKLLQQAQQMQAEMMQAQESLKDEVVEASAGGGMVKVKISGDL
                     VVQDVVIDPDAVDPEDSEMLAEMVQAAINEAIRSAQQLAESKMPQMPNMPGMPGLGG"
     gene            317..913
                     /gene="recR"
                     /locus_tag="PLV77_00835"
     CDS             317..913
                     /gene="recR"
                     /locus_tag="PLV77_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNC14424.1"
                     /translation="MSDGPAPLARLTAELSQLPGIGRRTAQRLAFHILRSDESDARSL
                     AQAIVEVKEKVGLCEICFNLAEGPRCRICEDHSRDESLICVVEEPADVIPIERTHEYR
                     GRYHVLGGALSPIDGIDAEDLRIAELRRRVEAGGISEVILATNPTTTGEATASHIADL
                     LRTQTKVTRLAAGLPVGADLEHADEVTLGRAMAGRGSI"
     gene            complement(922..1971)
                     /locus_tag="PLV77_00840"
     CDS             complement(922..1971)
                     /locus_tag="PLV77_00840"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC14425.1"
                     /translation="MSEHIWYTHAPSPTAFGIALRKGWIHEEMARCGVEVRALAASSD
                     PDVQRLRRERTSPRAFRQGGNVAPLIARSRGNDVRLIGLSRTSSIHPVLALPESGYRT
                     AADLLGRRIAIPVRKNAVVDIPRATALRTVSNLLASAGLTLNDVELVAIPVEGSYFEA
                     RAKPGEPIADAPREGVAFQVSSAHSNELLALIRGEVDAIASEGSTAAIIASTFGLTTL
                     EPGRAVNRDLNNRSLLAVTVSGGLLDRGPGLVTSVLVQILAAAEWSIDEEAEARRLIA
                     LDAGIHEDLLDAAYSPRVSRQLGVDLRMDKVADLREQHDFLLDRGIIERPVDLDEFIE
                     PEPLKTALARRGVLV"
     gene            complement(2053..2865)
                     /locus_tag="PLV77_00845"
     CDS             complement(2053..2865)
                     /locus_tag="PLV77_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC14426.1"
                     /translation="MPTGFEQDTAAEPLGEGRYAINLSDRWWVGRGPNGGYVAALMLR
                     AMTGEVASMEPEAALPPRSLSVHFLLRPEAGPAEIEVTVEHAGRVTAFLSARMVQSGE
                     VKAKAMAVFSGEREGPAFDNSLMPKVPPPEELEEWDTEQAPVAVFGRYRAMMIKGIPG
                     EAGERAETMGWIRLRDEQPVDPVLAAAVLDVWYPAPFVVFEKIPFAPTLEYTVHFPRT
                     IPEPGPPDWNLIRLTAEEGTEGHFTEDAELWSRDGKLLAKARQMALLRHPLG"
     gene            complement(2868..3224)
                     /locus_tag="PLV77_00850"
     CDS             complement(2868..3224)
                     /locus_tag="PLV77_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNC14427.1"
                     /translation="MTDGSLRCPECGSEHTYELGGFSVCSLCAHEWDPAHDRTEIAGD
                     SAPEIRDSVGNVLTDGDTVTVIKNLKVKGASGPIKAGTKVKGIRLVNGVGDHDIDCRI
                     DGFGQMQLKSSVVKKA"
     gene            3290..4573
                     /locus_tag="PLV77_00855"
     CDS             3290..4573
                     /locus_tag="PLV77_00855"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNC14428.1"
                     /translation="MPERDDIVVMKFGGTSVAGPEEIKRAARRMVAAREAGNSVVAVL
                     SARGKTTDELVASAYEISDRPDPREMDMLLSTGERISCALAAMAINDMGHKAVSLTGS
                     QAGIVTDSSHTKAKIIDVKADRIREGLGEDKIVLVAGFQGVSTDSRDVTTLGRGGSDT
                     TAVALAAALGASVCEIYTDVTGVFSADPRIVPDARKLPVVSFEEMLDMAASGAGVLQL
                     RSVEYARNYNIPIHCRSSFEDLPGTLVLPESETMERPIITAVTHSTDEARVAVIGVKD
                     EPGIAGRIFGALADANVNVDVIIQNEPVHEGALADLSFTVETGDLATATDAIASLGDI
                     SQEVRTDEQIGKVSIVGAGMRSHPGVAAKVFEVLGEKEINIEMISTSSIRISCVIQAD
                     QVPEAVRSLHAAFDLGEEQILAEAPTGEHRPVTPS"
     gene            4576..>4884
                     /locus_tag="PLV77_00860"
     CDS             4576..>4884
                     /locus_tag="PLV77_00860"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936746.1"
                     /note="catalyzes the formation of 4-aspartyl phosphate
                     from aspartate 4-semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNC14429.1"
                     /translation="MDESRYRVGVLGATGAVGSTILEILHERSFPASEVVAFASERSA
                     GKKVPFGEGELTCVVPDDESIQGIDILISSAGGSVSAEWAPKFSEAGAVVVDNTSYWR
                     M"
BASE COUNT          899 a         1589 c         1559 g          837 t
ORIGIN      
        1 ccggttgaat ggacatgaac aagctgcttc agcaggccca gcagatgcag gccgagatga
       61 tgcaggccca ggagtcactc aaggacgaag tcgtcgaagc cagcgccggc ggcggcatgg
      121 tcaaggtgaa gatcagcggc gacctggttg tccaggacgt ggtgatcgat cccgacgccg
      181 ttgatcccga ggattcggag atgctcgcgg agatggtcca ggccgcgatc aacgaggcca
      241 ttcgctccgc ccagcagctg gccgaatcga aaatgcccca gatgccgaac atgcccggca
      301 tgcccgggct cggtggatga gcgacggccc ggcaccactg gcccgcctga ccgccgagct
      361 ctcgcagctg ccgggaatcg ggcgccgcac cgcccagcga ctcgccttcc acatactcag
      421 gtcggatgaa tccgacgccc ggagcctcgc gcaggcaatc gtcgaggtca aggagaaggt
      481 cggtctctgt gagatctgtt tcaacctggc cgaaggtccg cgttgccgga tttgtgaaga
      541 ccactcgagg gacgaatcac tgatctgcgt cgtcgaggaa cccgcggacg tgattccgat
      601 cgagcggacc cacgaatacc ggggccgcta ccacgtgctc ggcggagcgc tctccccgat
      661 cgacggcatc gacgccgagg acctgcggat cgcggagctg cgtcgccggg tcgaggcggg
      721 agggatcagc gaggtgatcc tcgccaccaa cccgaccaca accggagagg cgaccgcctc
      781 acacatcgcg gacctgctcc gaacccagac gaaggtgacc cgcctggccg ccgggctgcc
      841 ggtcggcgca gacctcgaac atgccgatga ggtgacactt ggccgggcga tggctggccg
      901 agggtcgatc tagatcgggc ctcagaccag gaccccccgg cgggcaaggg cagtcttcag
      961 gggttccggt tcgatgaatt catcgaggtc cacgggtcgc tcgatgattc cgcggtcgag
     1021 caggaagtcg tgctgttccc ggagatcagc gactttgtcc attctcaggt ccacgccgag
     1081 ttgccggctg acccgagggc tgtaggccgc gtccaggagg tcctcgtgga tgcccgcgtc
     1141 gagggcgatc aggcgcctcg cttcagcctc ttcgtctatt gaccactcgg cggcggcaag
     1201 gatctggacc agaacgctgg tgaccagacc cggcccgcgg tcgagcagcc cgccacttac
     1261 cgttaccgcc agcagcgagc ggttgttgag gtcgcggttg actgctctgc cgggctcgag
     1321 ggtggtcagg ccgaacgtcg aggcgatgat cgccgcggtc gagccctcgg atgcgatcgc
     1381 atcgacctca cccctgatca gcgcgagcag ctcattgctg tgcgcggagg aaacctgaaa
     1441 ggcgacgcct tccctggggg catcggcgat cggctcaccc ggcttcgccc tcgcctcgaa
     1501 gtaggagccc tcgaccggga ttgccacgag ctccacgtcg ttcaacgtga ggccggccga
     1561 ggcgagcagg ttgctgactg tccggagggc tgtggccctc gggatgtcga ccacggcgtt
     1621 cttcctcaca ggaatcgcga tgcggcggcc gagaaggtcc gctgctgtcc ggtagccgga
     1681 ctccgggagg gcgagcaccg ggtgaatgct cgaggtgcgc gaaaggccga tcagcctcac
     1741 gtcgtttcct cgggagcggg cgatgagcgg agctacgttt ccgccctggc gaaaggccct
     1801 gggggaggtg cgctcgcgcc tcaggcgctg gacatccggg tcagacgagg ccgccagggc
     1861 cctgacctcg actccgcacc gggccatctc ctcatggatc cagcccttcc tcagggcaat
     1921 cccgaaagca gtgggtgacg gggcgtgtgt gtaccagatg tgctcgctca ttcggggcat
     1981 tgtagtcaaa cttgaccaag aaagtcataa atgttggcta aaggatgaag cggcttggtc
     2041 ggaccctggg gatcagccca atgggtgccg taggagagcc atttggcgcg ccttggcgag
     2101 cagcttgcca tcccggctcc agagctcggc atcctcggtg aagtggcctt cggttccctc
     2161 ttcggcggtc aaccggatca ggttccagtc cggcggaccg ggctcgggga tggttcgcgg
     2221 gaagtgcacc gtgtactcca gggtcggagc aaagggaatc ttctcgaaaa cgacgaacgg
     2281 cgccggatac cagacgtcga gcaccgcggc cgcgaggacg gggtcgaccg gctgctcatc
     2341 acgcagccgg atccagccca tggtttctgc cctctcaccg gcttctccgg gaatgccctt
     2401 gatcatcatc gcgcggtaac ggccgaaaac cgcgaccggg gcctgctcgg tgtcccattc
     2461 ctcgagttct tccgggggcg gaaccttcgg catcagggaa ttgtcaaagg ccggcccttc
     2521 acgctcaccc gagaaaactg ccatcgcctt cgctttcact tcaccgctct ggaccatgcg
     2581 ggcagaaaga aacgcggtca ctcttcccgc atgctcgacc gtgacctcga tctcagcagg
     2641 accggcttcg ggccggagca ggaagtggac gctgagcgac ctgggaggca gtgccgcctc
     2701 cggttccatc gacgctacct caccggtcat tgcccggagc atcaaggctg cgacatagcc
     2761 gccgttgggt ccgcgaccaa cccaccagcg gtcactcagg ttgatcgcgt agcgaccctc
     2821 gcccagcggc tcggccgccg tgtcctgctc gaacccggtc ggcattctca ggccttcttg
     2881 accacgctgg acttcagctg catctgcccg aaaccatcga tccggcagtc gatgtcatgg
     2941 tcgccgacac cgttgaccag ccggattccc ttcaccttgg tccccgcctt gatcggcccc
     3001 gaagccccct tgaccttcag gttcttgatc acagtcaccg tgtcaccgtc ggtgagcacg
     3061 ttcccgaccg agtcacgaat ctcgggggcc gaatccccgg cgatctcggt gcggtcgtga
     3121 gccggatccc actcgtgggc gcaaagcgag cagacggaaa aaccgccaag ttcataggta
     3181 tgttcgctgc cgcattccgg gcagcggaga gatccgtcgg tcacccggcg caggatattg
     3241 aaatgcgctt ggggacccgg ttaaccggtg atccgtatgc tcaggggtga tgcctgaaag
     3301 agatgacatc gtcgtaatga agttcggcgg gacctcggtc gccggcccgg aagagatcaa
     3361 gcgtgccgca aggcgaatgg tcgctgcccg cgaagccggc aacagcgtcg tcgcggtgct
     3421 atccgcccgg ggcaagacga ccgatgaact ggtcgcctcg gcctacgaga tctccgatcg
     3481 tcccgatccc cgcgagatgg acatgctgct ctcgaccggt gagcgaattt cctgcgccct
     3541 ggccgcgatg gcgatcaacg acatggggca caaggcggtc tcactcaccg gctcgcaggc
     3601 cggaatcgtg accgactcct cccatacgaa ggccaagatc atcgacgtca aggccgaccg
     3661 catccgtgag gggctggggg aagacaagat cgttctggtc gccggattcc agggtgtatc
     3721 aaccgacagt cgggacgtga ccacactcgg acgcggtggg tcagacacga ccgcagtcgc
     3781 gctcgcggcc gccctcggtg cgtcagtttg cgagatctac accgacgtga ccggcgtctt
     3841 cagcgctgac ccccgcatcg tccccgacgc gagaaaactt cctgtcgtct ccttcgagga
     3901 aatgctcgac atggccgcct ccggcgccgg agtactccag ctccgttcgg tcgagtacgc
     3961 ccgcaactac aacatcccga tccactgccg aagcagtttc gaggacctcc ccggtaccct
     4021 cgtacttccc gaaagcgaaa ccatggaacg accgatcatc acagccgtca cccactccac
     4081 cgacgaagcc cgcgtggccg tcatcggagt aaaggacgag cccggaattg ccggccgtat
     4141 cttcggcgcg ctagccgatg cgaacgtcaa cgtggacgtg atcatccaga acgagccggt
     4201 gcacgagggt gctctcgcgg acctctcgtt cacggtcgag accggtgatc tggccacggc
     4261 caccgacgcg atcgccagcc tgggcgacat cagccaggaa gtccgcaccg acgagcagat
     4321 cggcaaggtg tccatcgtcg gcgccggaat gcgcagccac cccggcgttg cagccaaggt
     4381 tttcgaggtt ctgggcgaaa aggagatcaa catcgagatg atctccacct cgtcgatccg
     4441 gatttcatgt gttatccagg ccgatcaggt gccggaagcg gtgcggtccc tccacgccgc
     4501 cttcgacctc ggcgaggagc agatcctggc cgaagcgccg actggcgaac atcgtcccgt
     4561 aaccccgtcc tgatcatgga tgaatccagg tacagggtcg gcgtcctcgg cgccaccgga
     4621 gcggtcggct cgaccatcct cgagatcctt cacgagcgtt cattcccggc cagcgaggtg
     4681 gtggctttcg cctccgaacg ctcggccggc aagaaggttc cctttggcga gggagaactg
     4741 acctgcgtgg ttcctgacga cgagtcgatt cagggcatcg acatcctgat ctcctcggcg
     4801 ggcggatccg tgagtgccga atgggctccg aagttctctg aagccggcgc ggtggtggtt
     4861 gacaacacct cttactggcg catg
//
LOCUS       DAOQMF010000028         4861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8153_length_4861_cov_3.594257, whole genome shotgun sequence.
ACCESSION   DAOQMF010000028 DAOQMF010000000
VERSION     DAOQMF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8153_length_4861_cov_3.594257"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..491)
                     /locus_tag="PLV77_00865"
     CDS             complement(57..491)
                     /locus_tag="PLV77_00865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC14430.1"
                     /translation="MTDTPPTRDGYPHFLTIPTRWKDNDIYGHVNNVEYYSYFDTVIN
                     EYLIREGGLDIHGGGTIGVCAESHCRFLAAVAFPDPVEAGLRVGHLGRSSVRYEIGLF
                     AGDGEPLAEGWFVHVFVQRESRRPDGIPDSIRSALEKLEVHS"
     gene            551..1438
                     /locus_tag="PLV77_00870"
     CDS             551..1438
                     /locus_tag="PLV77_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC14431.1"
                     /translation="MAASNESRSPHPSPSLPAVAMVLGSISSIQVGAALATGLFDEVG
                     AMGVTLLRTLISAVLLVLIWRPSFRLDRGRTRIALLFGFVLAAMNLSFYEAIDRIPLG
                     IAVTFEFIGPLGVALITSHRRRDLIWVAMAAAGILLLSGGIGEEGIELFGVVLAVVAG
                     VFWGCYILLGKRVGEEFYGGQGLAIAMVFSTVICTPFGVADGGADLLLPSVLAVGFAV
                     AILSTTLPLSLEMEALRRLPSNVFGVMMSLEPAMAATIGFLLLGQDMSAAQIAAIALV
                     VSASAGALSSARAPAPIEP"
     gene            complement(1435..2091)
                     /locus_tag="PLV77_00875"
     CDS             complement(1435..2091)
                     /locus_tag="PLV77_00875"
                     /inference="COORDINATES: protein motif:HMM:NF025510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HNC14432.1"
                     /translation="MSVRREMRGFLVGSLLFALGATPGYLGLVGVTADNLTYFLGSIF
                     FTIAGFTQLRLTGRWQRGGWSDRDAWADWWAAAIQFLGTLAFNVSTLAALLGTIDPGT
                     FERLGWRPDFIGSICFLVASVLAVEATTNRESLWDPEARNWWNTWLNMAGSVAFMISA
                     IFAWINPSTGEPLNTDWANLGTFFGAIGFFAAAALLKPQADADSNPSMNGTGPAENGD
                     "
     gene            2171..2243
                     /locus_tag="PLV77_00880"
     tRNA            2171..2243
                     /locus_tag="PLV77_00880"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2204..2206,aa:His,seq:gtg)
     gene            complement(2296..3198)
                     /locus_tag="PLV77_00885"
     CDS             complement(2296..3198)
                     /locus_tag="PLV77_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14433.1"
                     /translation="MKSGITVGLAALVACAGLVSSAHAAFPGKNGRIIFERKASQFAG
                     NSDPWVFSGGKARRLVRISNNAYNFSYSPNGKKIVFEATVPSEELVVMNANGTRPRVI
                     TAKVHKCIGKRHPTWSPNGKEIAFVCLNRNSLSDHDVWSVNANGTGIRRISRTHDAYS
                     PAWSPRGNKIAYTTYGGSIYTVPARGGKSKLLIAEAPGGVFGGTFQGVDWSPNGRLLV
                     SDSSGDGVYTINARTGATSGDLAEIGAEPVFSPDGKKILYVGIAESRGSTRIGLWKMN
                     LDGSNKQQVTRGGYDRAPSWAPAR"
     gene            3427..4053
                     /locus_tag="PLV77_00890"
     CDS             3427..4053
                     /locus_tag="PLV77_00890"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNC14434.1"
                     /translation="MKESDPDKTEILTASLKSAAAIMEHESIPYLLGGGLGTWAHGGP
                     PSSNDIDLMVRPADAERAIQAFADAGLRTERPPEQWLVKAWDGDVLIDLIFQPAGMRI
                     TEQVIERAEELNVGGMVVPVMCIDDILTTKLLAMDEHAADFLTLIQIARALREQIDWP
                     SLVERTSSSPFARAFFVLVDGLGIQPLTLPGPGGVEWQIGPGRQGIGP"
     gene            complement(4035..4316)
                     /locus_tag="PLV77_00895"
     CDS             complement(4035..4316)
                     /locus_tag="PLV77_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14435.1"
                     /translation="MATCQIRLVVLHYSPLRATVEGEPPEIFPFLGSDRMARPLLEHG
                     PDLVVHGHAHEGTHEGSIGEVPVRNVSLPVLGGEIWITELNAPVHGPIP"
     gene            complement(4396..4752)
                     /locus_tag="PLV77_00900"
     CDS             complement(4396..4752)
                     /locus_tag="PLV77_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC14436.1"
                     /translation="MAAVGDIHCREANREPLARSIAAISDADLLLIAGDITSHGTVEE
                     AQILCEVTAGVDFPVFAVLGNHDLHAGQSKEIIETLRAGGIDLLDGTSAISEIKGVEG
                     CRQPGLRRRVRAASPA"
BASE COUNT          776 a         1577 c         1631 g          877 t
ORIGIN      
        1 ggtctcggcg aagggggagg gcagtcccat ctccctcaga acggtggccg tggttttcac
       61 gaatggacct ccagtttctc gagtgcggag cggatcgaat ccgggatgcc gtcggggcgg
      121 cgactttcgc gctgaacgaa cacgtggacg aaccagccct cggcaagcgg ttcgccgtcc
      181 ccggcgaaga ggccgatctc gtagcggacc gaagatctac cgagatggcc gaccctcagg
      241 cccgcttcga ccggatcggg aaaggcgacc gccgccagga agcgacagtg ggattcggcg
      301 cagaccccga tcgtcccgcc gccgtggatg tccagcccgc cctcgcgaat cagatactcg
      361 ttgatgaccg tgtcgaagta cgagtagtac tcgacgttgt tgacgtggcc gtagatgtcg
      421 ttgtccttcc agcgggtggg gatcgtcagg aagtgcggat acccgtcccg ggtcggcggc
      481 gtgtcggtca tggggggatt attcctgagg gctcgtggca cggttatttc tccggtgatt
      541 cactgccctg atggccgctt ccaacgaaag ccgatcacct catccctcgc cgtccctgcc
      601 ggcggtggcg atggtgctcg ggtcgatcag ctcgatccag gtcggagcgg cgctcgccac
      661 cggtctcttc gacgaggtcg gggcgatggg cgtaaccctc ctcaggacgc tgatctcggc
      721 ggtgctgctg gtcctgatct ggcgaccaag cttccgtctg gaccggggcc ggaccaggat
      781 cgccctgctc ttcggtttcg tgctggctgc gatgaacctc tccttctacg aggcgatcga
      841 ccggatcccg ctcggcatcg ccgtgacctt cgagttcatc gggccactcg gggttgccct
      901 gatcacctcc caccggcgcc gggacctgat ctgggtcgcg atggcggcgg ccgggatcct
      961 gctgctttcc ggtggcatcg gcgaagaagg cattgagctc ttcggggtcg tcctcgccgt
     1021 ggtggccggt gtgttctggg gctgttacat cctgctcggc aagagggtgg gggaggagtt
     1081 ctacggcggg cagggactgg ccatcgcgat ggtcttctcg accgtcatct gcacgccctt
     1141 cggggttgcc gacggcggcg cggacctgct gctgccttcg gtcctggcgg tgggcttcgc
     1201 ggtggcgatc ctcagcacga ccctgccgct ctcactcgag atggaggcgc tccgccgcct
     1261 gccctcgaac gtcttcgggg tgatgatgag cctcgagccg gcaatggccg cgacgatcgg
     1321 cttcctgctc ctcggccagg acatgtccgc ggcccagatc gcggcgatcg cgctggtcgt
     1381 ctcggcctcg gccggcgcgc tttcctcggc ccgcgccccg gccccgatcg agccctaatc
     1441 cccgttttcg gcggggccgg tgccgttcat tgaagggttg gagtcggcgt cggcctgggg
     1501 cttgagcagg gcggccgcgg cgaagaagcc gatcgctccg aagaaggtgc caaggttggc
     1561 ccagtccgta ttcagcggct cgccggtgga gggattgatc caggcaaaga tcgccgagat
     1621 catgaaggcg accgagccgg ccatgttcag ccaggtgttc caccagttgc gggcttccgg
     1681 atcccacagc gattcgcggt tggtggtcgc ctcgaccgcg agcaccgagg caacgaggaa
     1741 gcagatcgaa ccgatgaagt cggggcgcca gcccaggcgc tcgaaggtgc cgggatcgat
     1801 cgtgccgagc agggctgcca aggtggaaac gttgaaggca agggtgccga gaaactggat
     1861 tgccgctgcc caccagtcgg cccaggcgtc gcggtccgac cagccgccgc gttgccagcg
     1921 gccggtcagc cgcagctggg tgaagccggc aatcgtgaag aagatcgagc cgaggaagta
     1981 ggtcaggttg tccgcggtga cgccgaccag tccgagatag ccgggagtcg cgccgagggc
     2041 aaagaggagc gaaccgacaa ggaagccccg catttcgcgg cgcacactca tcgcgcatct
     2101 cccgggcttg ctggctgcgg caccggcgca gcgtatccga gagccgaccg gtagatttct
     2161 tcccctcgcg gtgggcgtag ctcagttggt agagctcctg gttgtggtcc aggcggtcgc
     2221 gggttcgatc cccgtcgctc acccctcagg cagtgcgaac ggggccggtt ccaggctgaa
     2281 ccgaccccga ttccgttagc gggcgggtgc ccagctgggt gcccggtcgt agccgccccg
     2341 ggtgacctgc tgcttgtttg agccgtcgag gttcatcttc cagaggccga tcctggtgct
     2401 tcccctggac tccgctatgc ccacgtagag gatcttcttc ccgtcgggcg agaagaccgg
     2461 ctcggccccg atttctgcga gatcgccgga ggtggccccg gtccgggcgt tgatcgtgta
     2521 gacgccgtcg ccactcgagt ccgagaccag aaggcgcccg ttcggcgacc agtcgacgcc
     2581 ctggaaggtg ccgccgaaca cgccgccggg agcctccgca atgagcagct tgctcttgcc
     2641 gccccgggcc gggaccgtgt agatcgaccc gccgtaggtc gtgtaggcga tcttgtttcc
     2701 gcgcggcgac caggccgggg aataggcatc gtgggtcctg gaaatccggc ggatgccggt
     2761 tccgttggcg ttgaccgacc acacgtcgtg atcggagagg ctgttccggt tgaggcagac
     2821 gaaggcaatc tccttgccgt tcggagacca ggtcgggtgc cgcttgccga tgcatttgtg
     2881 aaccttcgcg gtgatcaccc gcggccgcgt gccgttcgcg ttcatgacga caagctcctc
     2941 cgagggcacg gttgcctcga agacgatctt cttgccgttc ggcgagtagg agaagttgta
     3001 agcgttgttg ctgatcctga cgagccgcct cgccttgcca cccgagaaca cccagggatc
     3061 cgagttgccc gcgaactggc tggccttgcg ttcgaagatg atcctgccgt tcttgcccgg
     3121 aaaggcagca tgcgctgacg agacgagccc ggcgcacgcg accagcgccg ccagacctac
     3181 ggtgattcct gacttcatcc ttctgttttc ccttctccag accgaacccg ggcagagttc
     3241 cacgcagggc tttccccgcg actggtccgc caaagccggc cggtgatcaa ttaactcgag
     3301 cccccaaccc gatccatcaa ttcgccgccg attctagcga ttcgggctgc ggcaaagtcc
     3361 gcccgggaag gcggcgcacc ggaccgtttt ctcctcgctt gacgggggta cccggcgggt
     3421 tgcggcatga aagagagcga ccccgacaag accgagatcc tgaccgccag cctgaagtcg
     3481 gcggcagcga taatggagca cgaaagcatt ccctacctcc tcggaggggg cctcgggacc
     3541 tgggcgcatg gggggccgcc ttccagcaac gacatcgacc tgatggttag gcccgccgac
     3601 gcggaacggg cgatccaggc attcgccgac gccggccttc gcaccgaaag gccacccgag
     3661 caatggctgg tcaaggcctg ggacggcgac gtcctgatcg atttgatctt ccagccggcc
     3721 ggcatgcgga ttaccgagca ggtgatcgaa cgggctgaag aactgaacgt gggcggcatg
     3781 gtggtgccgg tgatgtgcat cgacgacatc ctcacgacca agttgctggc catggacgag
     3841 catgccgccg acttcctcac cctgatccag attgcccggg cgctccgcga gcagatcgac
     3901 tggccgtctc tggttgaacg gacttcctcg agtcctttcg cgcgggcctt cttcgtgctg
     3961 gtcgacgggc tcggcattca gccgctcacc ctgcccggcc ccggcggtgt cgaatggcag
     4021 atcggcccgg gccgtcaagg gatcgggccg tgaacggggg cgttcagttc ggtgatccag
     4081 atctcgcccc cgagcaccgg cagcgacacg ttgcgcaccg ggacctcgcc gatgctgcct
     4141 tcgtgcgttc cctcgtgggc atggccgtgg acgaccagat ccggcccgtg ctcaagcagg
     4201 ggcctcgcca tccggtcgct gccgaggaac gggaagatct ccggaggctc gccttcgacc
     4261 gtcgcccgaa gcggggaata gtgaaggacg acgagccgga tctggcaggt cgccacttca
     4321 ctcagaccgg cctccagggc ggaaacctcg cgggaggtct cctcgtagac ggcgcgtagc
     4381 gagggttcgc cgaagtcagg caggtgatgc ggcgcgaacc cgccgacgaa gccccggctg
     4441 ccgacaaccc tcgacgccct tgatctcgct gatcgctgaa gttccgtcga gcagatcgat
     4501 gccgcccgcc ctcaaggtct cgatgatctc cttcgactga ccggcatgaa ggtcgtggtt
     4561 gccgagcacc gcgaacacgg gaaagtcaac cccggcggtc acctcgcaga ggatctgtgc
     4621 ctcctcgacg gtgccgtgac tcgtgatgtc cccggcgatc agcagcaggt ccgcgtccga
     4681 aatcgccgcg atgctccggg ccagcggttc acggttggcc tcgcggcagt gaatgtctcc
     4741 aactgccgcc actcggatcg atctgtcctc ttcggccatc ggcattgcgt ccagccagaa
     4801 gggttacccc gtttgccccg gttcattcag gtccggccgg gtaccgcgcg gcatgtggga
     4861 c
//
LOCUS       DAOQMF010000029         4825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8258_length_4825_cov_4.345283, whole genome shotgun sequence.
ACCESSION   DAOQMF010000029 DAOQMF010000000
VERSION     DAOQMF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8258_length_4825_cov_4.345283"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..328
                     /locus_tag="PLV77_00905"
     CDS             194..328
                     /locus_tag="PLV77_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14437.1"
                     /translation="MDNDIVRRLTWSVMLTASSALASLIAARLAAVAYRRFFNEDPPE
                     "
     gene            332..799
                     /locus_tag="PLV77_00910"
     CDS             332..799
                     /locus_tag="PLV77_00910"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNC14438.1"
                     /translation="MAPEDYQPPPYQRPPGRPDPNRPASEKTVGEMVFEVSERTSNLI
                     REEIELAKTEVSEKVTTLARGSAVGIAAGVFAFLALILLMHAFALGVASLFGWGAWAG
                     YLLEGVIFIAIAGGAGYFAYSSFEKTGAPIPTEAIEEAQKTKALLTPGEDSNE"
     gene            792..1187
                     /locus_tag="PLV77_00915"
     CDS             792..1187
                     /locus_tag="PLV77_00915"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HNC14439.1"
                     /translation="MSDEKPETGASGSENPVEGVEKPFRPEGVGPEAPVDRTPNRHVA
                     PEGTPAPAAGAAGSPYSPPPPKRNSTQIRADIDAQRAELGKSVDQLRDRVTELTDWKS
                     QLRKHRKQIIVGAVATGFVVGGLMALRRR"
     gene            complement(1662..2183)
                     /gene="def"
                     /locus_tag="PLV77_00920"
     CDS             complement(1662..2183)
                     /gene="def"
                     /locus_tag="PLV77_00920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC14440.1"
                     /translation="MAVREVLTYPDPILKSVCSPAQSGPDDREETGRVITDLLDTMNS
                     FDHCVGLAAPQIAESVRIAVVDITGHPRAKEPHGLMVLVNPRVVHRSEGTKVSREGCL
                     SLPDLTANVRRPRKATVEFLDGQRNLEMNQVQLAGFEARCVLHEIDHLDGILFLDRVA
                     SITDDLFRRQNYG"
     gene            complement(2217..2744)
                     /locus_tag="PLV77_00925"
     CDS             complement(2217..2744)
                     /locus_tag="PLV77_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNC14441.1"
                     /translation="MNLAVTAIGRDRPGIVAAITSALLEAGGNVDDSQMSILHGHFAV
                     MLIVSVPADASLESLEARLAEVGREFDLGGITVSPVEGLDRGAGPTHVLTVYGADRPG
                     IVHDTAATLASLGVNITDLRTRRTGDPDSPLYTLMVEVAVPEGTGPELDQALDGLRQS
                     KQLEARLAELESEVF"
     gene            complement(2788..3675)
                     /locus_tag="PLV77_00930"
     CDS             complement(2788..3675)
                     /locus_tag="PLV77_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNC14442.1"
                     /translation="MSAESTIALVTDSTASLTREDRERLEISVVNLYVILNGEQRPEN
                     EIDDYAAFYKAVLESPQLTTTSQPSIGDFMEVYGPLLDEGREIVSLHLSGAISGTCEA
                     ARQAAVQLEADGRGGERITVVDSRTTAGGLAFCNLAAANAIKDGKDVAGVVAQIEACR
                     EEIDTLILVDTLEYLRKGGRIGGAQAWIGGALKIKPLITLRETVEPVDKVRTLSRAMQ
                     RLKDHASEKAASGQEITWTAQHIQAPELAAELAAHCREVFQCEGAFLDEMSTVLGCHA
                     GPGLVAIGTVPTDAVGSKF"
     gene            3776..4147
                     /gene="erpA"
                     /locus_tag="PLV77_00935"
     CDS             3776..4147
                     /gene="erpA"
                     /locus_tag="PLV77_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNC14443.1"
                     /translation="MSEQPESAITLTEAAAEKIAELIGDSPEGEEQSLRVAVRGGGCS
                     GFQYALAFDTRGEDDHVFEHRGVSVIVDKVSMQFVFGSEVDYVEGLTGAGFQVNNPNV
                     VAACGCGSSFQVKDDAEAATV"
     gene            complement(4278..4682)
                     /gene="grxD"
                     /locus_tag="PLV77_00940"
     CDS             complement(4278..4682)
                     /gene="grxD"
                     /locus_tag="PLV77_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNC14444.1"
                     /translation="MADQELTDRVRELINENSVLLFMKGTPEAPRCGFSMRTVQVLDQ
                     MGVEYGAIDVLPALQPLREVTSEISDWETFPQLYVNGELVGGCDIVEEMFDNGELAEA
                     LGVEQPEPAEPDFETSAPGEPYKSPPMNIEGA"
BASE COUNT          813 a         1591 c         1583 g          838 t
ORIGIN      
        1 ctcctggtca aggcgatctg cgaaggcctg gaacgccgct gacgcccggg cggcgccggg
       61 aacccccgac cgagggtttg ttcctgcctg tcttgttgaa cctcaggtag ccacgggatc
      121 gcccgtgatc ctccgacccc gcggaccgtt tccgtattcg ttctccggag gcttgggtat
      181 tgtggtttga ccaatggata acgacatcgt cagaagactc acctggagcg tcatgctcac
      241 cgcgagcagc gccttggcga gcctgatcgc cgcccggctt gctgcggttg cctaccgccg
      301 gttcttcaac gaggacccgc ccgaatagac cgtggctcca gaggactacc agccgccgcc
      361 ctaccagcga ccacccggcc gcccggatcc gaatcgcccg gcatccgaaa agaccgtcgg
      421 ggaaatggtc ttcgaggttt ccgagcgcac ctcgaacctg atccgtgaag agatcgagct
      481 cgccaagacc gaggtcagcg agaaggtcac caccctggcc cgcggctcgg cggtcggcat
      541 cgccgccgga gtcttcgcct tcctcgcact gatcctgctg atgcacgcct tcgcgctcgg
      601 cgtcgcctcg ctcttcggct ggggcgcctg ggccggctac ctgctggaag gcgtgatctt
      661 catcgcgatc gccggcggcg ccgggtattt cgcctacagc tctttcgaga agaccggcgc
      721 cccgatcccg accgaggcga tcgaggaagc ccagaagacc aaggccctgc tgacccccgg
      781 agaagattcc aatgagtgac gaaaagcccg agacgggcgc atccggatcg gagaatccgg
      841 tcgaaggtgt cgagaagcct ttccgtcccg agggcgtcgg cccggaggcc ccggtcgacc
      901 gcacaccgaa ccgccatgtc gctcccgagg gcaccccggc cccggccgcc ggcgcggcag
      961 gctcgcccta ctcgccaccg cctccgaagc ggaactcgac ccagatccgg gcggacatcg
     1021 acgcccagag ggccgagctc ggcaagtccg tggaccagct ccgcgaccgg gtgaccgagc
     1081 tcaccgactg gaagagccag ctccgcaagc accgcaagca gatcatcgtc ggcgcggtcg
     1141 ccaccggctt tgtagtcggc ggactaatgg ccctccgccg ccggtagaca gcccccgggc
     1201 accgcgccca ggaaccacca gccgtccgct agcttcgtcg tcggccggaa acgacagctc
     1261 atgtacccgc gtactatcgc ggtcatttcc ggcctgtctc cttgccagcg aacggctgga
     1321 ggcccctggg cacgacccgc tcacggttgg tggtgttgac atttcgcgag atgcggtttg
     1381 tcggcatata gcgccgacaa gcagcatctc gatcgattcc tgaccgtgat tcggggccgg
     1441 tgccgcaggt ttccgacctc agaggttgca aacctgcggc accagcctgg atagggacgc
     1501 gacctggccg tgaagccagc tcggacccgg gatcccttca acttccccct tcacctgccc
     1561 tgttccaccg cagtgggtcg gatttccgtc gtagcagcca gaaaaccgac ccaccggacc
     1621 atccgaaccg gtcctcgaca gcgcagtggg tcggttgcgg gttagccgta gttctggcgg
     1681 cggaagagat cgtcggtgat tgaggcgacg cggtcgagga agaggattcc gtcgaggtgg
     1741 tcgatctcgt ggaggacgca gcgggcctcg aagccggcca gctggacctg gttcatctcg
     1801 aggttcctct ggccgtcgag aaactcgacc gtggcctttc gggggcggcg aacgttggct
     1861 gtgaggtcgg gcagcgagag acagccctcg cggctgacct tcgttccttc cgagcggtga
     1921 accacccggg ggttgaccag caccatcagc ccgtgcggtt ctttcgccct gggatggccg
     1981 gtgatgtcga ccacggcgat ccggaccgac tccgcgatct ggggcgcggc caggccgacg
     2041 cagtgatcga aggaattcat cgtgtcgagc aggtcggtga tcacccgccc ggtttcttcg
     2101 cggtcatccg gtcccgactg ggcaggggaa cagaccgact tgagaatggg gtcgggatag
     2161 gtgaggacct cgcgaactgc caacggttgc ctgcctcaga ccgcggagcc ggccacttag
     2221 aagacctcgg actcgagttc ggcgagccgg gcttccagct gcttcgactg gcgcagcccg
     2281 tcgagggcct ggtcgagttc cggaccggtt ccttccggca ccgctacctc gaccatcagc
     2341 gtgtagaggg gcgaatcggg atcgcccgtg cgccgggttc gcaagtcggt gatgttgacg
     2401 ccaagtgaag ccagcgtggc cgccgtgtca tggacgatcc cgggacggtc ggccccgtag
     2461 acggtgagta catgggtcgg tccggctcca cgatccagcc cctcgaccgg actgaccgtg
     2521 atgccgccca ggtcgaactc gcggccgacc tcggccagcc tcgcctccag cgactcgagc
     2581 gaggcatcgg cgggaaccga gacgatcagc atcacggcga aatggccgtg gaggatcgac
     2641 atctgcgagt cgtccacgtt gccgcccgct tcgagcaggg cgctggtgat tgcggcgacg
     2701 atgccgggac ggtcgcggcc gatcgcggtg acggccaggt tcacgggcgc agcctcgccc
     2761 gccaggcccg cggtagcgcc gcagtcatca gaacttcgag ccgaccgcgt cggtgggcac
     2821 cgtgccgatc gcgaccagcc ccgggccggc atgacatccc agcacggtcg acatctcgtc
     2881 gaggaatgct ccttcgcact gaaagacctc gcggcagtgg gcggccagtt cggccgccag
     2941 ttccggagcc tggatgtgtt gggcggtcca ggtgatttcc tgcccggagg cggctttctc
     3001 gctggcgtga tccttcagcc gctgcatggc acgggagagg gttcgcacct tgtcgaccgg
     3061 ctcgacggtc tcgcggaggg tgatcagcgg cttgatcttg agcgccccgc cgatccaggc
     3121 ctgggccccg ccgatccggc cgccctttct caggtactcg agggtgtcaa ccaggatcag
     3181 cgtgtcgatc tcctcgcggc aggcctcgat ctgggcgacg accccggcca cgtccttgcc
     3241 gtccttgatc gcgttcgcgg cggcaagatt gcagaaggcc aggccgccgg cggtggtgcg
     3301 ggaatcgacc acggtgatcc gttccccgcc gcgaccgtcg gcttcgagct ggaccgcggc
     3361 ctgccgggcg gcttcgcagg ttccggagat cgcgcccgag aggtgcagcg agacgatctc
     3421 cctgccctcg tcgagaagcg gcccgtagac ctccatgaaa tccccgatcg agggttgcga
     3481 agtggtggtg agctgcggcg attcgagcac cgccttgtag aaggcggcgt agtcgtcgat
     3541 ctcgttctcg ggccgctgct cgccgttgag gatcacgtag aggttgacca cgctgatttc
     3601 gaggcgctca cgatcctccc gggtgagcga cgcggtcgaa tcggtcacca gcgcgatggt
     3661 ggactcggcg gacacccaac ggttctatca gacaccgggc cgggtcgggc aaagcaggcg
     3721 ggaaaactgt tcccccgtca cagaccacgc cgggcacacg cgctactctt taaccatgtc
     3781 cgagcagccc gaatccgcaa tcaccctgac tgaagccgcc gccgagaaga tcgccgaact
     3841 gatcggtgat tctcccgagg gcgaggagca gtcgctgcgc gttgcagtgc ggggtggagg
     3901 ctgctcgggg tttcagtacg cgcttgcctt cgacaccagg ggtgaagacg atcatgtctt
     3961 cgagcatcgc ggggtttccg tgatcgtcga caaggtcagc atgcagttcg tcttcggctc
     4021 ggaagttgac tacgtcgagg gtctgaccgg ggccgggttc caggtcaaca acccgaacgt
     4081 cgtagctgcc tgtggctgcg ggtcttcctt ccaggtgaag gacgacgccg aagcagcgac
     4141 tgtttaaccg catgcattcc ggtccgcgcc ggaatgccac agaaacccgt ccgatcacgg
     4201 catcaatgaa ttgatgatcg gactccgtca gttgacggtc ccttaagctc gcctacggca
     4261 cccacgccgg ttaagcgcta cgcgccctca atattcatcg gcgggctctt gtacggctcg
     4321 ccgggagcgg aggtttcgaa atccggttcg gcgggttcgg gctgctcgac cccgagagcc
     4381 tcggccagtt cgccgttgtc aaacatttcc tcgacgatgt cgcagccgcc aaccagctcg
     4441 ccgttcacat agagctgggg gaaggtctcc cagtcggaga tttcgctggt tacttcgcgc
     4501 aggggctgaa gggcgggcag tacgtcaatt gctccgtact cgactcccat ctggtcgagc
     4561 acctgaacgg tgcgcatcga aaacccgcag cgcggggcct cgggggtgcc cttcatgaag
     4621 agcagcacgg agttctcgtt gatcagttcg cggacgcgat ctgtgagttc ctggtcagcc
     4681 atgtaactga tgttagaccc tggccactct ccggacccgt gcggcgcggc gcggtcaacg
     4741 gggaagggcc cgggcggtca gcgaattcgg ccggaggggc cgaaattccg gaagcctgcc
     4801 ccgcacggcg ttagactcga tcgcc
//
LOCUS       DAOQMF010000030         4824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8262_length_4824_cov_4.092682, whole genome shotgun sequence.
ACCESSION   DAOQMF010000030 DAOQMF010000000
VERSION     DAOQMF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8262_length_4824_cov_4.092682"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLV77_00945"
     CDS             <1..305
                     /locus_tag="PLV77_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNC14445.1"
                     /translation="YSWGYPACPDQSEHEKVWELLDLGEIGMYLSGGYAVMPEQSTVA
                     IIAHHPQAVYFGMKSGFIPKGEAPDEVIAESDRSANPPEADPDEGPTEAEGEPATA"
     gene            complement(295..720)
                     /locus_tag="PLV77_00950"
     CDS             complement(295..720)
                     /locus_tag="PLV77_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14446.1"
                     /translation="MKKAPLFLVAALASAALLAGGSPAGAALQGSPKPLKVKLVTFDG
                     KQRLKVKRKVRLIASCSKDCNARVKLRLVTPVTTVRGGGSIGLEATGTWTIGVILSPS
                     FRSYIRQNITRCRLVANFSAVDIETGKTVRKVKTFRFRR"
     gene            882..2864
                     /locus_tag="PLV77_00955"
     CDS             882..2864
                     /locus_tag="PLV77_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC14447.1"
                     /translation="MVPTAAASSAPHLSLPAGDSTDNGNVYLGDSSVITVTVNSDGSS
                     PLDITSVSIYAFPGNSEYFSIEQDNCSGQSVESGDHCTFQVRFTASVVGHKFTSVRID
                     SNDVESGGMKFFGISGTGINPGIDADAASHNFGTVQVGEYGTDKTFTIENDGTSDLNA
                     TATITGTDAAEFFFSSQPNCASVTPDSTCQIIVNLRPLGSAGPRSANLVIANNTKNEN
                     PVTIPLTATASAPHFTSEPLGLGFGQFDLFDDPPATRPLTIKSDGTADLKINGMVIDP
                     LNSNSNAFTLDQGECVDDLAIAPGDDCTLNITFKAGSFGEGSYNTSLLVFTNNGDQSV
                     PVSAARTSGGMSTDSSADVGDVELGQTGSATLTLTSTGTAPLKMSPPVIDGPDAGMFS
                     VDQPSDCNGLASGQSCEVTVHFTPTSLAGKQAGLTLSGNFGSTGVLLTGSGTQAFVNV
                     PGDITFPTTIVGKQVTWDESLISNGSAPFSLGSLTIEGADASMFSVETTGSCASVAHG
                     DECPYSITYKPTSTGSHEAQLKITGNLAGGSRTIPLSGTARNLTPAKVSLKLTGAKQV
                     KPGKTVTLTARISKSGEEQTGDLTLKTVVPKKMAASVKPVRIPHLGAGNRTVTRKITI
                     KVRRSAKSGQKLKVEATVSGGGLNKAGKAGKTVTIR"
     gene            complement(2857..3267)
                     /locus_tag="PLV77_00960"
     CDS             complement(2857..3267)
                     /locus_tag="PLV77_00960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC14448.1"
                     /translation="MAAETTHRLRVRYNECDRVGIVFNSNYLVYTDVAVTEFWREHLG
                     GYSEFNEIGLDMVLAEANLRFRQPLEFDDEFDIRVSVGGMTARSMTIEFEFTRSGEAI
                     AEIEISYVCVSSTEHTPQPIPDSIRTVLEGDSAT"
     gene            complement(3257..4168)
                     /locus_tag="PLV77_00965"
     CDS             complement(3257..4168)
                     /locus_tag="PLV77_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC14449.1"
                     /translation="MTRQDQEKKLTVDLPQGTVAFRDEGEGPVLLFSHGIFVDSSLWD
                     GLVERLVPAGFRCVRPETPMGAHRIPLNPETPLSPPRVADLLADFIDALDLGPVTVVG
                     SDSGGAVSQMFAAQHPDKLAAMVLTNCDALEVYPPFPFTLLPPLARMPGGIAPLGLLM
                     KLRPVRWATYRMLTADPIPDRQLKAWLGPTGSNPGIRRDVNKVIGGLEKNQAIDAAEK
                     LRGFEKPVLFAWGESDRFFKPELAERLAAMIPNSRIEKVEGGRTFVALDDPEKVSNLI
                     VEFMNETVAPAGRTDAQRSTLAGADGR"
     gene            4230..>4824
                     /locus_tag="PLV77_00970"
     CDS             4230..>4824
                     /locus_tag="PLV77_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC14450.1"
                     /translation="MQAARTQIERSDATRRALIDSARNLFGSRGYAAVPLDEVVSAAG
                     VTKGALYHHFPGGKKDLMEALYETVEADFTERVTAGIAPRLAEGEVDPLDIMGAAIEE
                     TLDLSMDPELQQILLLDSLAVLGWSRWREIADKYSLAVVKSLLAAAVEHGSMKPLPTE
                     SLANLLMAALSEAILMIPRAGDQEQAKADASRSLKAII"
BASE COUNT          935 a         1597 c         1474 g          818 t
ORIGIN      
        1 gctactcatg gggctacccg gcctgcccgg accagtccga acacgagaag gtctgggaac
       61 tgctcgacct gggcgagatc ggcatgtacc tctcgggcgg ctacgccgtg atgcccgaac
      121 agtccacggt cgcgatcatc gcccaccatc cgcaggcggt ctatttcggc atgaagtccg
      181 gtttcatccc caagggagaa gccccggacg aggtcatcgc cgaatccgac cgctcggcca
      241 atccgcccga ggccgatccg gacgaaggcc cgaccgaagc cgagggcgaa ccggctaccg
      301 cctgaagcgg aaggtcttga cctttcgtac ggtcttgccc gtttcgatgt ctaccgccga
      361 gaagttcgcc accagcctgc atctggtgat gttctggcgg atgtagctgc ggaaagacgg
      421 actgaggatc acgccgatcg tccaggtgcc cgtggcctcg aggccgatcg atccgccgcc
      481 gcggacggtg gtcaccgggg tgaccagccg gagcttgacc cgcgcgttgc agtccttcga
      541 gcaggaagcg atcaggcgaa ccttccgttt gaccttgagc cgctgcttgc cgtcaaaggt
      601 gaccagcttc accttgagcg gcttcgggct gccttgaagt gcggctccgg cgggtgaccc
      661 gccggcgagc aaggcggctg aggccagggc ggccacaaga aacaagggcg ctttcttcat
      721 cgattcctcc cggcggttga gaactgttcc cagcctaagg caccagaccg cacccgttca
      781 ttccgtacgg tgttcataaa gtaggatcag ctcaaccgac caagggagtt tggatgttcg
      841 ccaggggttt cctagtttcg cttttcgctg cgttcgcatt gatggttcca actgctgccg
      901 cttcgtctgc gcctcacctg agcttgcccg cgggggactc gaccgacaat ggcaacgtct
      961 acctcggtga ttccagtgtg atcaccgtga cggtgaattc agacggatcc agtcccctcg
     1021 acatcacttc ggtctcgatt tacgcgttcc cggggaactc cgaatatttc tcgatcgagc
     1081 aggacaactg ctcgggtcaa tcggtggagt ccggcgacca ctgcaccttc caggtcaggt
     1141 tcaccgcttc cgtggtcggc cacaagttca cttcggtcag gatcgacagc aacgacgtgg
     1201 aaagtggcgg catgaagttc tttggcatca gcggtaccgg catcaaccca gggatcgacg
     1261 cggatgccgc gagtcacaac ttcgggacgg tccaggttgg tgagtacgga actgacaaga
     1321 ccttcaccat cgagaacgac ggaacctcgg acctgaacgc caccgcgacc atcaccggta
     1381 ccgatgctgc cgagttcttc ttcagttccc agcccaactg cgcctcggtc acgccggatt
     1441 ccacctgcca gatcatcgtg aatcttcgcc cgctcgggag cgccggcccc cgctccgcca
     1501 acctggtgat cgccaacaac acgaagaacg agaacccggt cacgatcccg ctgaccgcca
     1561 ccgccagcgc ccctcatttc accagcgagc ctctgggact ggggttcggt cagttcgacc
     1621 tgttcgatga cccgcccgcg accaggcccc tgaccatcaa gtccgacgga accgcggacc
     1681 tgaagatcaa cggcatggtc atcgaccccc tcaactcgaa ctccaatgcc ttcaccctcg
     1741 accaggggga atgcgtggac gacctggcga tcgcacccgg tgatgactgc acgctcaaca
     1801 tcactttcaa ggcaggttca ttcggcgagg gctcctacaa caccagcttg ctggtcttca
     1861 cgaacaacgg cgaccagtcg gttccggtca gtgccgcccg gacctccggc ggcatgtcca
     1921 ccgactcatc ggcagacgtc ggggacgtgg agctcggcca gaccggatcg gcgaccctga
     1981 ccctcacttc gaccggaacc gcgccgctga agatgtcacc gcccgtaatc gacggccccg
     2041 acgcgggcat gttctcggtc gaccagccga gcgactgcaa cggtctcgcc agcggccaga
     2101 gctgcgaggt caccgtccac ttcaccccca cctcgctggc cggcaagcag gccggcctga
     2161 ccctctcggg caacttcggc agcacgggag tgctcctgac cggctccggc acccaggcct
     2221 tcgtcaacgt cccgggtgac atcaccttcc cgacaacgat cgtgggcaag caggtgacct
     2281 gggacgagtc cctgatttcc aacggttcgg ccccattcag cctcggctca ttgaccatcg
     2341 agggagccga cgcgtcgatg ttctcggttg agacaaccgg cagttgcgcg agcgtcgccc
     2401 acggcgatga atgcccgtac agcatcacct acaagccgac ctcgaccgga agccacgaag
     2461 cgcagctgaa gatcaccgga aacctggcag ggggatcccg gacgatcccg ctctccggca
     2521 ccgccaggaa cctgactccg gcgaaggtct cgttgaagtt gaccggagcc aaacaagtga
     2581 agcccggaaa gaccgtgacc ctgaccgcca ggatctcaaa gagtggcgag gagcagaccg
     2641 gagacctgac cctcaagacg gtggttccga agaagatggc cgcctcggtg aaaccggtca
     2701 ggatccccca cctcggagca ggcaaccgga ccgtcaccag gaagatcacg atcaaggtca
     2761 ggaggagcgc gaagagcggc cagaagctga aggtcgaggc gaccgtgtcc ggcggcggcc
     2821 tgaataaggc cggcaaggcc gggaagaccg tgacgatcag gtagccgagt caccctcaag
     2881 cacggtgcgg atgctgtccg gaattggctg gggagtgtgt tcggttgacg agacgcagac
     2941 gtaggagatc tcgatctcgg cgatcgcctc gccgctccgg gtgaattcga actcgatcgt
     3001 catcgagcgc gcggtcatgc cgcccacgct cacccggata tcgaactcgt cgtcaaactc
     3061 gagcggctgc cggaagcgga ggttcgcctc ggccaggacc atgtcgaggc cgatctcgtt
     3121 gaactccgaa taaccaccca ggtgctcgcg ccagaactcg gtaaccgcca cgtcggtgta
     3181 gacgaggtag ttcgagttga agacgatccc gacccggtcg cactcgttgt aacggaccct
     3241 cagcctgtgg gtggtttcag cggccatcag ctcccgcgag agtacttcgt tgcgcatccg
     3301 tccgaccggc cggggcgacc gtctcgttca tgaattcgac gatcaggttc gagaccttct
     3361 cggggtcgtc gagggcgacg aaggtcctgc cgccttcgac cttctcgatg cgggagttcg
     3421 ggatcatcgc ggccagccgc tcggccagtt ccggcttgaa gaaacggtcg ctttcacccc
     3481 aggcgaagag caccggcttc tcgaatcccc gtagcttctc ggcggcgtcg atcgcctggt
     3541 tcttttccag cccgccgatc accttgttta cgtcccggcg gattccggga ttgcttccgg
     3601 tcgggcccag ccacgccttc agctggcgat ccgggatcgg atcggcggtc agcatccggt
     3661 aggtggccca gcgcaccggc ctgagcttca tcagcaggcc gagcggggcg attccaccag
     3721 gcatccgggc gagcggcgga agcagcgtga agggaaacgg cggatagacc tcgagtgcat
     3781 cgcagttggt gagcaccatc gccgccaact tgtcgggatg ctgcgcggca aacatctgac
     3841 tgacggcacc gccggaatcg ctgccaacca cggtcaccgg accgagatcg agcgcatcga
     3901 tgaaatcagc gagcaggtcc gccacgcggg gcggggagag aggcgtctcg gggttgagcg
     3961 gaatccggtg ggcacccatc ggagtctccg gccgcacgca gcgaaacccg gccggaacca
     4021 ggcgctcgac cagcccgtcc cagagcgatg agtcgacgaa gatcccgtgc gagaagagga
     4081 gcaccggccc ctccccctcg tcccggaatg cgaccgtgcc ctggggcagg tcgacggtca
     4141 gtttcttttc ttggtcttgc cgggtcatcg gtcctcctca ggttgcgtac cgccggtatg
     4201 tattgccagc aagataacat acccgcggta tgcaagctgc gagaacccag atcgagcgct
     4261 cggacgcaac ccgccgcgcc ctgatcgatt cggcccggaa cctgttcgga tcgaggggct
     4321 acgcggcggt gcccctggac gaggtcgtct ccgcggccgg ggtcaccaag ggagccctct
     4381 accaccactt cccgggcggc aagaaagacc tgatggaggc gctttacgaa acggtcgaag
     4441 ccgatttcac ggagcgggtc accgccggca tcgcgcctcg actggccgaa ggagaggttg
     4501 acccgctgga catcatggga gcggccattg aagagaccct cgacctcagc atggatcccg
     4561 agctccagca gatcctcctg ctcgattcgc tagccgtcct cggctggagt cgctggcggg
     4621 aaatcgcgga caagtacagc ctcgccgtgg tcaagtccct cctcgccgcc gcggtggaac
     4681 acggctcgat gaaaccgctc ccgaccgaat cgctggccaa cctgctcatg gccgcccttt
     4741 ccgaagcgat cctgatgatc ccccgggccg gggaccagga gcaagcgaaa gccgacgcat
     4801 ccagatcact caaggcgatc atcg
//
LOCUS       DAOQMF010000031         4746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8470_length_4746_cov_3.465786, whole genome shotgun sequence.
ACCESSION   DAOQMF010000031 DAOQMF010000000
VERSION     DAOQMF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8470_length_4746_cov_3.465786"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1834
                     /locus_tag="PLV77_00975"
     CDS             194..1834
                     /locus_tag="PLV77_00975"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNC14451.1"
                     /translation="MRYPVESFTPEEQASLAPYFTNLDQPVFALTNLPETVKGALFAR
                     YSRYGGTLRRLYLDEFAADAAAADPEGRLRPFDGEEGQRAAKLYESVFVGFGDDSIAQ
                     LGGAHVACEWVSNILTKVLQRGRLAAYLEQSTRYIAYDQEMPGGGYRYLSGDALDGQF
                     EAAMNDIFLIYSDSLTRVEAWAAERWPRGDDEPEGPWRRSIKAKALDLLRGLLPASTL
                     SHVGIYASGQAWEQLLFRMMASPLPEARRFGGMILDELNKVIPSFVSRVSRPDRGGEW
                     ISFLEQRRQATEDWVERLGLTDRDSEDSGPTVELGHVHGTEDDLLAACLYEESDAPES
                     RIRERIARLDSRQREELMAAMIGERRNRRHRPGRGFESVSYRFEIVSDYGAFRDLQRH
                     RMLTCQWQTLTPDLGAGVPAEVVEAGVGGAYEEALEISRNEYERLRDAGHPEAAPYAL
                     CLGYRIRYILDLNAREAMQLIELRSGREGHPTYRAVAQAMHDEIAEVHPAIAGAMKHL
                     DTSMEPRLERMLSEIRTHHKQLAAAQTSKAPPGFSELG"
     gene            1944..2771
                     /locus_tag="PLV77_00980"
     CDS             1944..2771
                     /locus_tag="PLV77_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HNC14452.1"
                     /translation="MLSARIPPALPCALAFLLLAMMVVFAACSSDPDQSEAKPARWQP
                     KPTTAAWQFQLQGKIDTSIDAPVFEVDGFNVPKSTVTRLHSMGRKVICYIDVGSWENY
                     RPDAKRFPGSVIGRKYEGYPNERWLDIRRYRKFAGPIKSRIRMCARKGFDGLEPDNIN
                     GWENPTGFPLTARDQLRFNRWIAKVAHNNGLAVGLKNDGRQARKLVGAFDFAVVEQCF
                     QYSECGQFLPFIRRGKAVYSVEYERPNSAFCARAETLGFSSIGKEYDLFARPWRPCA"
     gene            complement(2911..3693)
                     /locus_tag="PLV77_00985"
     CDS             complement(2911..3693)
                     /locus_tag="PLV77_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNC14453.1"
                     /translation="MALADSYDGFLIDLDGVVWLGHEPIEGAAKAIAALAAAGKPFVF
                     VTNSPRVTPAEQARILREQGIEADEDRVVTAGDTLLELTRKEIGEAGKVYAIGTSGFL
                     EQVEGAGFELLPAERWQEAEAVLVTGHPRFDYDELKAASMAARAGAFLATIGRDPTMP
                     MPDGLWPGTGSIVAAVEAASGRTAVVAGKPERPIFDLGVRRLGLPEGARVAMIGDRLD
                     TDVGGAQKAGLEGILVAGNESHNPETQVTPNHNISSLLDLTD"
     gene            3772..4263
                     /locus_tag="PLV77_00990"
     CDS             3772..4263
                     /locus_tag="PLV77_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC14454.1"
                     /translation="MTGNRTHTEGPPPFETALEDHGPRLHAWLLARVGPSRADDVFQE
                     TMLAALAAWDRVPVQSVRAWLFTIARNKAIDEQRRDANRPRPEGEVDSWPSAEVAERL
                     EDPVWDQVRELPEKQRIAVTLRFRGDLNHREIGTVMEISEAAARRNVFEGVKRLRERI
                     EDE"
     gene            4256..>4746
                     /locus_tag="PLV77_00995"
     CDS             4256..>4746
                     /locus_tag="PLV77_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNC14455.1"
                     /translation="MSEIEARLAAGEVAGTGSNWEEIRTSLLRQADREGAIDLAYERH
                     DTPFGTMVIVAGEGGILRIALAAEPETEVLTDLARRVSPRIGRTGRDALTDARRQLDD
                     YFGGELRTFDLPLDWRLTRGFRREVLHETAAIPYGATASYAQMARQAGSPRAVRAAGS
                     ALAT"
BASE COUNT          794 a         1611 c         1561 g          780 t
ORIGIN      
        1 gatgcgccgg gggcacaacc ggatcaccgt ccttgcggcc ggggcgggcg ggtctctgcg
       61 ccgggtttcc gaggactgaa cccgccccgt cggggccgcg accggccagc ccggcgacgc
      121 cggacttcgc gatcgggtcc gggtctacat aacccggcag tcgagcgtcg ggcgcgcctg
      181 ttaggttcga cgggtgcgct acccggtcga gagctttact ccggaggagc aggcctcgct
      241 cgctccctat ttcaccaacc ttgaccagcc cgtgttcgcg ctcacgaacc tgcccgaaac
      301 ggtcaaggga gccctcttcg cccgctattc ccgttacggg gggaccctgc gaaggctcta
      361 tctcgacgag ttcgcagccg acgccgcggc cgccgacccg gaagggcgcc tcaggccctt
      421 cgacggggag gaaggccagc gtgcggcgaa gctctacgag agcgtcttcg ttggtttcgg
      481 tgacgattcg atcgcccagc tcggtggtgc ccacgtcgcc tgcgaatggg tgtcgaacat
      541 cctgaccaag gtgctgcagc gcggtcggct ggctgcctac ctcgagcagt ccacccgcta
      601 catcgcctac gaccaggaga tgcccggtgg cggctaccgc tacctgagcg gcgacgctct
      661 cgacggccag ttcgaagcgg cgatgaacga catcttcctg atctactcgg attcactcac
      721 ccgggtggaa gcctgggccg ccgagcgctg gccgcgcggt gacgatgagc ccgaaggccc
      781 ctggcgccgt tcgatcaagg ccaaggcact ggacctgctg cggggccttt tgccggcttc
      841 gactctcagt catgtcggga tctacgcctc gggccaggcc tgggagcagc tgctgttccg
      901 gatgatggca tcgccgttac cggaggcccg tcgcttcggc gggatgattc tcgacgagct
      961 gaacaaggtg atccccagtt tcgtttcccg ggtaagccgt cccgatcgcg gcggcgagtg
     1021 gatttccttt ctcgagcagc gccgccaggc gaccgaagac tgggtcgaaa ggctcggcct
     1081 cacagaccgc gacagcgaag acagcggacc gacggttgag cttggccatg ttcacggcac
     1141 cgaagacgac ctgctcgcgg cctgccttta cgaggagtcc gacgctcccg aatcgcggat
     1201 tcgggaacgg atcgcccggc ttgattcccg ccagcgcgaa gaactcatgg cggcgatgat
     1261 cggcgagcgc cgcaaccgtc gccaccgccc cggtcgcggg ttcgagtcgg tctcgtaccg
     1321 cttcgagatc gtctccgact acggcgcctt ccgggatctt cagcgccatc gcatgctgac
     1381 ctgccagtgg cagaccctga ctccggatct cggggccggg gtaccggccg aagtggtgga
     1441 agccggggtc ggcggtgcct acgaagaggc gctcgagatc tcgcgaaacg agtacgagcg
     1501 actgcgcgac gccggccatc ccgaggccgc cccttacgcg ctctgcctcg gctaccggat
     1561 ccgttacatc cttgacttga acgccaggga ggcgatgcaa ctgatcgagc tccgatcagg
     1621 cagggaaggc cacccgacct accgggcggt cgcccaggca atgcatgacg aaatcgccga
     1681 agtccatccc gccatcgccg gtgcgatgaa gcacctcgac acctcgatgg agccccgcct
     1741 cgagcgcatg ctctccgaga tccggaccca ccacaagcaa ctcgccgccg cccagacctc
     1801 caaggccccg cccggcttct ccgaactcgg ctgatccggc cagcggccaa accggccgcg
     1861 agatccgact ttttcttcac gatggcgaag aactcacgcc tcaccgtccc cggccggtgt
     1921 gcccgattcc ctaacctgtc ccgatgctct ccgcccggat tcccccggcg cttccctgcg
     1981 cgctcgcctt cctgctgctg gcgatgatgg tcgtattcgc agcctgttct tccgaccccg
     2041 atcagtccga ggcgaagccg gcccgctggc agccgaaacc gacgaccgca gcctggcagt
     2101 tccagctcca gggcaagatc gacacctcga tcgacgcgcc ggtattcgag gtcgacggct
     2161 tcaacgtgcc gaagtcgacc gtgaccaggc ttcactcgat gggtcgcaag gtgatctgtt
     2221 acatcgacgt cggcagctgg gagaattacc gccccgacgc gaaacggttc cccgggtcgg
     2281 ttatcggcag gaagtacgag ggatacccga acgagcgctg gctcgacatc cgccgctatc
     2341 ggaagttcgc ggggccgatc aagtcaagga tccggatgtg cgcccgcaag ggattcgacg
     2401 ggcttgagcc ggacaacatc aacggctggg agaacccgac cggattcccg ctgaccgccc
     2461 gggaccagct tcgattcaac cggtggatcg ccaaggtcgc gcataacaac ggccttgccg
     2521 tcggcctcaa aaacgacgga cggcaggcgc gcaagctggt cggcgccttc gatttcgccg
     2581 tggtcgagca atgcttccag tacagcgagt gcggccagtt tctgcctttc atccgtcgtg
     2641 gcaaggccgt ttactcggtc gaatacgaac ggcccaattc cgccttttgc gctcgtgcag
     2701 agacgcttgg gtttagttcg attggcaagg agtacgacct cttcgcacgg ccctggcgcc
     2761 cctgcgccta gcggtcacgc cgcgggatcg ggtgagcatc gccgggacgg acgcacgcgc
     2821 ggcattcagc cgcgctggtc ctggagtgtc ccggacgacg ctcacccgac cccgcggctg
     2881 cgcctacccg gcggattttc gcgggagggc tcagtcggtc aggtcgagga ggctggagat
     2941 gttgtggttg ggggtgactt gggtttcggg gttgtggctt tcgttgccgg cgacgaggat
     3001 tccttcgagg ccggccttct gggcgccgcc gacatcggtg tcgaggcggt ccccgatcat
     3061 cgccacccgg gccccttccg gcaggccgag ccgccggacg ccgagatcga agatcgggcg
     3121 ttccggcttg cccgccacca ccgcggtccg gcccgaggcc gcttcgaccg cggcgacgat
     3181 tgaacccgtc ccgggccaga ggccatccgg catcggcatg gtcgggtcgc ggccgatcgt
     3241 cgcgaggaag gcgccggccc gcgccgccat cgaagcggcc ttcagttcgt cgtaatcgaa
     3301 gcggggatgg ccggtcacca gcaccgcctc cgcctcctgc cagcgttcgg cgggaagcag
     3361 ctcgaaacct gcgccttcga cctgctccag aaatccgctt gtaccgatcg catagacctt
     3421 gccggcctcg ccaatctcct tccgggtcag ctccagcagg gtgtcgcccg cagtcaccac
     3481 ccgatcttcg tcggcttcga ttccctgctc gcgcagaatc ctcgcctgct cggccggcgt
     3541 cacccggggg ctgttcgtga cgaagacgaa cggcttgccc gcggcagcca gcgcggcaat
     3601 ggccttcgcc gctccttcga tcggctcgtg accgagccag acgaccccgt cgaggtcgat
     3661 caggaagccg tcgtatgagt cggccagggc cacgagccaa caatagagtt gcggcatgag
     3721 tccggtcggc ggcgatctca caaaacctgc ctcggcttcg tctcaggggc agtgaccgga
     3781 aatcgcaccc acaccgaggg gccgccccca ttcgagacgg cccttgagga tcacgggccg
     3841 aggctgcatg cctggctgct cgcgcgagtc ggaccctccc gggccgacga tgtctttcag
     3901 gagacgatgc tcgcggcgct ggccgcctgg gacagggtcc cggttcagtc ggtgcgcgcg
     3961 tggctcttca cgatcgcccg gaacaaggcg attgacgagc agcgccggga tgcgaacagg
     4021 ccccggccgg aaggcgaggt tgacagttgg ccttcggccg aggttgccga gcgcctggag
     4081 gatccggtct gggatcaagt gcgggagctg ccggagaaac agcggatcgc ggtgaccctt
     4141 cgctttcgcg gtgatctgaa ccaccgtgaa atcgggaccg tgatggagat ctccgaagcg
     4201 gccgcccgcc gcaacgtgtt cgaaggcgtc aagcgcctgc gggaaaggat cgaagatgag
     4261 tgagatcgaa gcgagactgg ccgccggcga agtggccggg accggaagca actgggagga
     4321 gatccggacc agcctgcttc ggcaggcaga ccgggagggt gccattgacc tcgcctacga
     4381 gcggcatgac acaccgttcg ggacgatggt gatcgtcgcg ggggaagggg gcatcctccg
     4441 gatcgccctc gccgcggaac cggaaaccga agttctcacg gacctcgccc ggcgggtttc
     4501 gcccaggatc ggacgcaccg gtcgcgatgc cctgaccgac gcccgccggc aactcgacga
     4561 ctatttcgga ggagagctca gaaccttcga cctgccgctc gactggcggt tgacccgggg
     4621 cttccggcgc gaggtgctcc acgaaacggc agcgattccc tacggtgcca cggcaagcta
     4681 tgcccagatg gccaggcagg ctggaagccc gcgggcggtc agggcggcag gctcggccct
     4741 cgccac
//
LOCUS       DAOQMF010000032         4726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8525_length_4726_cov_3.819953, whole genome shotgun sequence.
ACCESSION   DAOQMF010000032 DAOQMF010000000
VERSION     DAOQMF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8525_length_4726_cov_3.819953"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="PLV77_01000"
     CDS             complement(<1..1816)
                     /locus_tag="PLV77_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14456.1"
                     /translation="MAVLGLAAAISTGIGGVASAAPADRGNDPVVLKGSQVAGLDGVA
                     PGRIVGFKWEGKWVQVPVQVDQRHTVSARGLYPDEAPAYVNNDLTFDVEIYADAKTRS
                     GADADPNLDPDDEIAFMGSDTGDAAPAATLSPKGVIGSTATRIDVVDPIDTSVAQVYL
                     FKSDGSLDPSAGKDYVDYDFNLTNLGPGETLIDDYGFSNSNNPEDSTVSTANYTLHST
                     DRWMEDEIKIKAGGASDTDILDREAASAGGLGGCGRSEYTFSGNWNRGSDNDEGTILA
                     IKDGPVRAIRDYMGANSGPYVEDTHVYYSDREDRMIHVRVHPIPLMYVWTDYNDQAVG
                     MTYRDQLNPTGVKIDGVPGEQDGQPFDDSIDKATPADVPSGQYLWQQVTGAQGTATTL
                     VSADSSVEPGGIFNGFAGYYLDDSTPTGSSERQCGGDMKAYGASGFGIDGVYPNTDPN
                     LATTASPAAKLSVNRIRYFTSPNGTAADADGLRDRAQDPLTGSSADANIKARAAKLKV
                     TLQGPAPKAKPGRQLRLKLKIANSGTADSAEGKVCVAGVALPHKTCRNFARIFRQKST
                     TVTLTPTVKKTVKANKLVLKVTATSGKASDGLTLKLPLD"
     gene            complement(1874..3724)
                     /locus_tag="PLV77_01005"
     CDS             complement(1874..3724)
                     /locus_tag="PLV77_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14457.1"
                     /translation="MPRKFAQGLTAFGLAVAIIFGFTGNAAAAPADRSGDAVVLKGSE
                     IARLLGADPARLVGFSFRNGRWSQIPVQVDERHTVDVRSLYPPENRTYVGDSSPDFNL
                     EVYADPKTRSGADEDATFDANDELTFMGGDTGSRVPAGVGAPAPVVPGSGTAVRVSDP
                     VGGGNAWVYLFRSINRKFDPSAGKDYVDYDFKLTGLTGGQTLLNDYGYFSQFNPEDST
                     VKTDDYELHSYDRWMEDELKIRAGNANGTDILDREVAQAGRGSCGRSEYTFSGRWGQD
                     STSGNDNSTDNEGTYVAVIDGPVRAIRSYMGANSGPYVQREHIYYRDHEQNTIFLRVH
                     QMADLYTWTDFAPSAIGMTYRDAKNTAGVTVDGNPDSIAPTTSADISNGAYSWQQISG
                     PQGTATTVMGSDTDLTDPNFGNYYLDDSTPSGPLEVQCGGDGQSIGASGFGIFGEGDT
                     GITPNTDPRSAGFKTLTTRRTRYFGPPADGVAKAENYTARVSSPLTATSSPSPMKHGM
                     GSARAYILTRGVTAHRGRAIRLRVKVRNSGNFRIRKAKACVFGKGLTGRPCTRVNDLG
                     IGNERTVTLKTRVGRFAKGRKLKLSVSVTGSAGDWGFAGVGGYFLIPLRG"
     gene            complement(3769..>4726)
                     /locus_tag="PLV77_01010"
     CDS             complement(3769..>4726)
                     /locus_tag="PLV77_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC14458.1"
                     /translation="SCSIGIRFDPASAGAVTANLVIPNQSAYPDGNTVHPDISISLAG
                     TGAADPSLTITPSSYDFGQSGVNLSTPSQVFTVSNPARSSRYVDLVARVGSGAAHFLN
                     TSGNCVTRTLGPGASCTYRLAFKPTSPGLKNAVLEVRGSGGGSLASAAVQGTGISVAV
                     TGTTGQTGQTGPIGPTGNTGQTGPTGETTATGPTGPTGSTGETTTGPTGPTGPIEPTG
                     PTGPTEPVGPTGPTGPTEPTGPTGPTQPTGQTGPTGPVRPSTACLKSRRNLAAARKRL
                     ATAKKSLKKAKSQPAKKAARTRVMRIGKDVGRFQKSVARNCG"
BASE COUNT          691 a         1487 c         1619 g          929 t
ORIGIN      
        1 ggtcgagggg gagcttcagc gtcaggccat ccgaggcctt gcctgaagtg gcagtgacct
       61 tgaggaccag cttgttcgcc ttcacggtct tcttcacggt cggtgtgagg gtcaccgtgg
      121 tcgacttctg gcggaagatc cgcgcgaagt tcctgcaggt cttgtgcggg agggccacac
      181 cggccacaca gaccttgcct tcagccgagt ccgcggtacc gctgtttgcg atcttcagtt
      241 tcagcctgag ttgcctgccc ggcttcgcct tcggagcggg accctgaagc gtgaccttca
      301 gcttggctgc ccgggccttg atgttggcgt ctgccgacga accggtgagc gggtcctggg
      361 cgcggtcccg gaggccgtct gcgtcggcgg cggtgccgtt cgggctggtg aagtagcgga
      421 tccggttgac cgagagcttc gccgccggtg aagcggtggt ggcaaggttc ggatcggtgt
      481 tcgggtagac gccgtcgatg ccaaagccgc tggccccgta cgccttcatg tcaccgccgc
      541 actgcctctc ggaggagccg gtgggagtcg aatcgtcgag gtagtaaccg gcgaatccgt
      601 tgaagatgcc gccgggttcg accgagctgt ccgccgagac cagagtggtc gcggtgcctt
      661 gcgcgccggt cacctgctgc cagaggtact ggccgctggg cacgtcggcc ggcgtcgcct
      721 tgtcgatcga atcgtcgaag ggctgaccat cctgctcgcc gggaacgccg tcgatcttga
      781 cgccagtcgg gttcagctgg tcacgatagg tcatgcctac cgcctggtcg ttgtagtcgg
      841 tccagacata catgagcggg atcgggtgga cccgcacgtg gatcatccgg tcttcgcgat
      901 cggagtagta gacatgggtg tcctccacat acgggccgga gttggcgccc atgtagtccc
      961 ggatcgcgcg gaccgggccg tccttgatcg cgagaatcgt gccttcgtcg ttgtcgctgc
     1021 cgcgattcca gtttccggag aaggtgtatt cactccggcc gcagccaccg aggccaccgg
     1081 ccgaggccgc ctcgcggtcg agaatgtcgg tatcgctcgc gccacccgcc ttgattttga
     1141 tttcgtcttc catccagcgg tcggtggagt ggagggtgta gttggctgtg ctgaccgtcg
     1201 aatcttcggg attgttcgag ttcgagaacc cgtagtcatc gatcagggtc tcgcccggcc
     1261 cgaggttggt caggttgaag tcgtaatcga catagtcctt cccggccgag gggtcgagtg
     1321 aaccgtcgga cttgaagagg tagacctggg cgacgctggt gtcgatcgga tcgaccacat
     1381 cgatccgcgt cgcggtggaa ccgatgacgc ctttcggcga aagcgtggcg gccggagcgg
     1441 catctccggt gtcactgccc atgaaggcga tctcgtcatc cgggtcgaga ttggggtctg
     1501 cgtcggcgcc ggagcgggtc ttggcgtcgg cgtaaatctc gacgtcgaag gtcaggtcgt
     1561 tgttgacgta ggcgggcgcc tcgtcgggat agaggccgcg ggcactgacc gtgtggcgct
     1621 ggtccacctg aaccggaacc tggacccact ttccttccca tttgaaaccg acgatccggc
     1681 ccggcgcgac accgtcgagc cccgcgacct ggctgccctt cagaacgacc ggatcattgc
     1741 cccggtcggc cggagccgcc gacgcgacac caccgatccc ggttgaaatt gccgcggcga
     1801 ggccaagcac agccatcgac cgcgcgaacg tcttacgcgc gaggtcccat cccatgtcgg
     1861 gaaggatagc cgctcagccg cggagtggaa tcaggaagta accgccgacg ccggcaaatc
     1921 cccagtcacc cgcgctgccg gtgaccgaga cgctcagctt cagtttcctg cccttcgcga
     1981 accggccgac cctggtcttc aaagtcacgg tccgctcgtt gccgataccg agatcgttga
     2041 cccgggtgca tggcctgccg gtcagtccct tgccgaaaac acaggccttc gctttccgga
     2101 tccggaagtt gccgctgttt cgtaccttga cccggagcct gatcgcccgg ccacggtgcg
     2161 cggttacgcc ccgggtgagg atgtaggcgc gggccgaacc catgccatgc ttcatcgggc
     2221 tcgggctgct ggtcgcggtc agcgggctgc tcaccctggc cgtgtaattc tccgctttgg
     2281 cgaccccgtc agccggcggg ccgaaataac gggtgcgcct ggtcgtcaga gtcttgaaac
     2341 cggcgctgcg ggggtccgtg ttcggggtta tgcccgtgtc gccctcgccg aagatcccga
     2401 agccgctggc tccgatcgac tggccgtcac ccccgcactg gacctcgagc ggaccggacg
     2461 gagtcgaatc gtcgaggtag tagttgccga agttcgggtc cgtgagatcg gtatccgatc
     2521 ccatcaccgt ggttgccgtt ccctgggggc cggagatctg ctgccacgag taggccccgt
     2581 tcgagatgtc ggcgctggtg gtcggggcga tcgaatccgg attgccgtcg accgtgacgc
     2641 cggccgtgtt cttcgcgtcg cggtaggtca tgccgatcgc cgaaggcgcg aaatcggtcc
     2701 aggtgtagag atcggccatc tggtgaaccc ggaggaagat cgtgttctgc tcgtggtccc
     2761 ggtagtagat gtgctcgcgc tggacgtaag gcccggaatt ggcgcccatg taggagcgga
     2821 tcgcccgtac cggcccgtcg atcacggcga cataggtgcc ctcgttatcg gtcgagttgt
     2881 cgttcccgct ggtcgaatcc tggccccagc ggccggagaa cgtgtattcg gagcggccgc
     2941 agctgccccg tccggcctgc gcgacttccc ggtcgaggat gtcggttccg ttcgcgttgc
     3001 cggcccggat cttcagttcg tcttccatcc agcggtcgta cgagtggagc tcgtagtcgt
     3061 cggtcttgac ggtcgagtcc tcggggttga actggctgaa gtagccgtag tcgttcagca
     3121 gggtctgtcc cccggtcagt ccggtcagct tgaagtcgta gtcgacgtag tccttgccgg
     3181 ccgaagggtc gaacttccgg ttgatcgatc ggaagagata gacccaggcg ttgccgccac
     3241 cgaccgggtc gctcaccctg accgccgttc ccgagcccgg aacaaccggg gcgggagcac
     3301 caacgccggc cggaacccgg ctgccggtgt caccgcccat gaaggtcagc tcgtcgttgg
     3361 cgtcaaaggt tgcgtcctcg tcggcgcccg acctggtctt ggggtcggcg tagacctcga
     3421 gattgaaatc cgggctgctg tctcccacgt aggtgcggtt ctcgggcggg taaaggctgc
     3481 gcacgtcaac cgtgtggcgc tcgtcaacct gcaccgggat ctggctccag cgaccgttcc
     3541 tgaaggagaa gccgaccagg cgcgccgggt cggccccgag cagcctggcg atttcactgc
     3601 ccttgagcac gacggcatct ccggagcggt ccgccggtgc cgcggcggcg ttgccggtga
     3661 atccgaaaat gattgccacg gccaggccga aggcagtcag tccctgcgcg aacttcctgg
     3721 gcacgtttct ccttgatcgt tggtcgacgg tgatttgaac agaccaggtc agccgcagtt
     3781 gcgggcaacc gacttctgaa atctgccgac atccttgccg atcctcatca ccctggtccg
     3841 cgcggccttt ttcgctggct ggctcttcgc cttcttcagc gacttcttcg ccgtggccag
     3901 ccgcttgcgg gcggcggcga ggttccgcct ggatttgagg caggcggtcg acggcctgac
     3961 cggtccggtc ggcccggtct ggcccgtggg ctgagtcggc cccgtgggtc cggtcggttc
     4021 ggttgggccg gtcggcccgg tgggcccaac aggttcggtt gggcccgtgg gtccggtcgg
     4081 ttcgatcggt ccggtcggtc cggtgggtcc ggtggttgtc tcgccggtgc ttccggtcgg
     4141 cccggtcggt ccggtcgccg tggtctcccc ggtcgggccg gtctggcccg tgttcccggt
     4201 cggcccgatc ggcccggtct gaccggtctg gccggtggtt ccggtcacag cgacggagat
     4261 tccggtgccc tgaacggcgg cgctggccag ggacccgcca cctgatccac gcacctccag
     4321 aaccgcgttc ttcaaccccg ggctggtcgg cttgaaggcc aggcggtaag tgcaggacgc
     4381 acccgggccg agtgtcctcg tcacgcagtt gccgctggtg ttgaggaaat gtgcggcgcc
     4441 gctgccgacc ctggccacaa ggtccacata tcgcgacgag cgggccgggt tgctgaccgt
     4501 gaacacctga cttggggtcg agaggttcac accggactgc ccgaaatcat atgaggacgg
     4561 cgtgatggtc agcgaggggt cggccgcgcc ggtcccggcg agggagatcg agatgtcggg
     4621 gtgaaccgtg tttccgtcgg gatacgcgga ctggttcgga atcaccaggt tggcggtgac
     4681 cgcacctgcc gaagccgggt cgaaccgtat cccgatgctg caactc
//
LOCUS       DAOQMF010000033         4725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8529_length_4725_cov_3.925054, whole genome shotgun sequence.
ACCESSION   DAOQMF010000033 DAOQMF010000000
VERSION     DAOQMF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8529_length_4725_cov_3.925054"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLV77_01015"
     CDS             <1..553
                     /locus_tag="PLV77_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HNC14459.1"
                     /translation="QKKSRAEIAERRAETVAKVREVAPLEFVNGGGTGSLESTSAEEA
                     VTEVAAGSGFFAPAQFDHYSNLSLRPAAGYALPVVRRPEPRVVTALGGGYLASGPPDP
                     GRMPVPWLPEGLEFEKDEGAGEVQTPLVGEAAQELAIGDNVYLRHAKAGELCERFESL
                     ILIEGDEIVDEVPTYRGEGQTFL"
     gene            complement(576..1172)
                     /locus_tag="PLV77_01020"
     CDS             complement(576..1172)
                     /locus_tag="PLV77_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC14460.1"
                     /translation="MNPVDAGLPAGVTESVEVPRRADAVRNREKVIAAAEQVFAEHGI
                     EAGVPEIAEKAGVGKGTVYRNFETKDDLVAAVLIRRMNRFDEDILDALESDDPGAAFG
                     EALRKAATRASDLSFQAGIYWSGSSEELIEVKARVKGHMARLVRKAQKAGAIRKDATV
                     DEVWILFGGICRALGDAGEKDPKVWRRHTDLVVDAFRP"
     gene            complement(1169..2647)
                     /locus_tag="PLV77_01025"
     CDS             complement(1169..2647)
                     /locus_tag="PLV77_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC14461.1"
                     /translation="MSPFSQSSAGSDRPHPTRTLLILSLAALSFALAQTTLIPALAEL
                     MEEFHTDATGIAWVLTGYLISAAIFTPIFGRLGDMFGKRRLLVIALSIFAAGSTVSAL
                     GDSVPAIVAGRILQGVGGGIFPLCFGIIRDEFPPEKVSASVGLMSATLGIGGGIGLVV
                     GGLIVDHASYHWIFWLGAAMAVMAAAAALIFVPESPNRVPAKVDYRGAAVLAVGLSVP
                     MYAISEANNWGWTSTKTIGLILAGFVVITFWVWLQKRTREPLADVSLLAEPRVAMTNT
                     ATLLIGFGMFASFILIPQLVESPDSTGYGFGFSATQAGLVMLPAALMMLPAGPLSGHL
                     GARFGNRVPLALGGLISGIGLIGLGLDHGSALSIVLWNVLSSIGVGLAYAAMPNLIME
                     AVPLERTGEATGFNTLIRSVGSSLGSQVTASILTGTILAATGFPTSDGFRTAFLVGAG
                     VSIFAGLLALLIPRSGHRHAIFTEEIGAASVLPEPALAADER"
     gene            complement(2687..4018)
                     /locus_tag="PLV77_01030"
     CDS             complement(2687..4018)
                     /locus_tag="PLV77_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HNC14462.1"
                     /translation="MVGENYDARKLGSGGHAWTNWAGHQSCRPRDLIRVSGVGELAEV
                     VGAAGAAGRQITTVGSGHSFTPTALSDDLLLDISRISGVISADRDSGLVEVGAGTVLA
                     DLNRELDHLGLAMPNLGDIDRQTIAGAVSTGTHGTGRALPNISAQIEKARIMGADGEI
                     RELEPGSDGLLAARVAVGSLGVLVSVTLRTVPAFNLHRVDAPMDLDQVLAGFDRITEE
                     NEHFEFFVFPYTRTALTLIRNRTDQPLAPRSQADRFLGDVVVENGLGDLALRLTGRFR
                     SAIPKMARFSTGFMSQSEQIDVSHRVFANYRTIRFNEMEYALPAEHGPEALDRVLELI
                     ETENFPLAMPIECRISAGDDALISQAHERPTVYIAVHQHRTMEYEPYFREIERIFREY
                     DGRPHWGKLHFRTAEDLAPSFPRWADFQKVRDELDPNRNFSNAYVTEVLGA"
     gene            complement(4008..>4725)
                     /locus_tag="PLV77_01035"
     CDS             complement(4008..>4725)
                     /locus_tag="PLV77_01035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC14463.1"
                     /translation="EPVFSMVLVAALILITIAVTLPEPTGVESQPLGEAWRRIRQSDV
                     PSGVLMLAGPSVAFGLVVVIGPLRMDDLGASPFLIAAAFASGSVVEAVVGPVIGRISD
                     RVGRTLPYLIGAGALILALVGLGIFDRLSLLFAVVMVMSFGAGMAFTPASTLVTDTAT
                     AAGVNQGYASGASNVAWGGGQMIGAIGGGALAGAAGYLLPCLVAAAVMAGVGIVARSL
                     GIGPPVISDLSEEAGTADGR"
BASE COUNT          818 a         1554 c         1654 g          699 t
ORIGIN      
        1 gcagaagaag tcgcgggccg aaattgccga acggcgggcc gagacggtgg cgaaggtccg
       61 ggaggttgcg ccgctcgagt tcgtaaacgg cggcggtacc ggctcactcg agtcgacctc
      121 ggccgaggag gcggtgaccg aggtggccgc cgggtcgggc ttcttcgcgc cggcccagtt
      181 cgaccactac tcgaacctga gcctgcgacc cgccgccggt tacgccctgc ccgtggtcag
      241 gaggcccgag cctcgagtgg tcaccgcgct cggcggcggc tacctcgctt cggggccgcc
      301 ggacccgggc cggatgccgg tcccctggct gccggagggg ctcgagttcg aaaaggacga
      361 gggcgccggc gaggtccaga ccccgctggt cggggaggcc gcccaggagc tcgcgatcgg
      421 cgacaacgtc tacctccgcc acgccaaggc cggagagctc tgcgaaaggt tcgagtcgct
      481 gatcctgatc gagggcgatg agatagtcga cgaggtcccc acctaccggg gcgagggcca
      541 gaccttcctc taggcgggag gcgccggaag ttcccctagg gccggaaggc gtcgaccacc
      601 aggtcggtgt ggcgccgcca gaccttcggg tccttctcgc cggcgtcacc gagggcgcgg
      661 cagattccgc cgaagaggat ccagacctca tcgacggtgg cgtccttgcg gatcgctccc
      721 gccttctgcg ccttgcgaac cagccgggcc atgtggccct tgacccgcgc cttgacctcg
      781 atcagctctt cggagcttcc cgaccagtag atgcccgcct gaaagctgag atcgctggcc
      841 cgggtggctg ctttgcggag cgcctcaccg aaggcggcgc cgggatcgtc gctttccagg
      901 gcgtcgagga tgtcctcgtc gaagcggttc atccggcgga tcaggaccgc ggcaacgagg
      961 tcgtccttgg tctcgaagtt ccggtagacg gtgcccttgc ccaccccggc cttctcggcg
     1021 atctccggca cgccggcctc gatgccgtgc tcggcgaaga cctgttcggc cgcggcgatc
     1081 actttctcgc gattgcggac cgcgtcggcg cggcgcggaa cctcgacgct ttcggttacc
     1141 ccggcaggga gcccggcgtc gaccggattc atcgttcgtc ggccgccagg gcgggttcgg
     1201 ggagaacgct ggccgcgccg atttcctcgg tgaagatcgc gtggcgatgg ccggagcgcg
     1261 ggatcaggag ggcgagcaac ccggcgaaga tcgagactcc ggctccgacc agaaacgcgg
     1321 tccggaaccc gtcgctggtc ggaaagccgg tcgcggcgag gatcgtgccg gtcaggatcg
     1381 aggcggtgac ctgggagccc agcgaggagc cgaccgagcg gatcagcgtg ttgaagccgg
     1441 tcgcctcacc ggtgcgttcc agcggcactg cctccatgat caggttcggc atcgccgcgt
     1501 aggcgagtcc cactccgatc gaggagagga cgttccagag cacgatcgaa agcgccgaac
     1561 catggtcgag ccccagcccg atcagtccga tgccgctgat cagcccgcca agcgcgagtg
     1621 ggaccctgtt tccgaaccgg gctccgagat gcccggaaag cggcccggcc ggcagcatca
     1681 tcaaggctgc gggaagcatc accaacccgg cctgggtcgc cgaaaagccg aaaccgtagc
     1741 cggtcgaatc gggtgactcg accagctgcg gaatcaggat gaatgaagcg aacatgccga
     1801 agccgatcaa cagggtggcg gtgttggtca tcgcgaccct gggttcggcc agcagggaga
     1861 catcggccag cggctccctg gtccgcttct gcagccagac ccagaaggtg atcaccacga
     1921 atccggcgag gatcagtccg atcgtcttcg tgctggtcca gccccagttg ttggcctccg
     1981 agatcgcata catcgggacc gaaagcccga ccgccagcac ggctgcgccc cggtagtcaa
     2041 ccttcgccgg aacccggttt ggtgactcgg gcacgaagat cagcgccgcg gccgcggcca
     2101 tcacggccat tgccgcgccg agccagaaga tccagtggta ggaggcgtgg tcaactatca
     2161 gcccgccgac gaccaggccg atgccgccac cgatgccgag cgtcgccgac atcaggccga
     2221 ccgaggccga gaccttttcg ggcgggaact cgtcacggat gattccgaaa cagagaggga
     2281 agattccgcc gccgacgccc tggaggatgc ggcccgcaac gatggcgggg acgctgtctc
     2341 cgagggccga gactgtggag ccggcggcga agattgagag ggcgatgacg agcagccggc
     2401 gcttgccgaa catgtctcca aggcgcccga agatcggggt gaagatggca gccgagatca
     2461 ggtagccggt gaggacccag gcgatcccgg tcgcgtcggt gtggaactcc tccatcagtt
     2521 cggccagggc cggaatcagg gtggtctggg ccagcgcgaa ggaaagcgcg gccaggctga
     2581 ggatcaggag cgtgcgggtc ggatggggtc ggtccgaccc ggcggacgat tgactaaacg
     2641 gactcaccgg tccggatagt aacagccaag cggacccgcg ggtccgctaa gcgccgagta
     2701 cttcggtcac ataggcgttg ctgaagttcc ggttcgggtc gagctcgtca cgcaccttct
     2761 ggaagtcggc ccagcggggg aagctcgggg cgaggtcctc cgccgtccgg aagtggagct
     2821 tgccccagtg cggccggccg tcgtactcgc ggaagatccg ttcgatctcc cggaagtagg
     2881 gctcgtattc catggtccgg tgctgatgga ccgcgatgta gacggtcggc cgttcgtggg
     2941 cctggctgat cagggcgtcg tcaccggccg agatccggca ctcgatcggc attgccagcg
     3001 ggaagttttc ggtttcgatc agctccagta cccggtcgag agcctccggc ccatgctcgg
     3061 ccgggagcgc gtattccatc tcgttgaagc ggatcgtccg gtagttcgca aagacccggt
     3121 gtgaaacatc gatctgctcc gactgggaca tgaagccggt cgagaaccgg gccatcttcg
     3181 ggatggccga ccggaaccgc ccggtcaggc ggagggcgag gtcaccgagt ccgttctcga
     3241 ccaccacgtc accgaggaag cgatcggcct gcgagcgggg ggcgagcggc tggtcggtgc
     3301 ggttgcggat cagggtcagg gcggtccggg tgtaggggaa gacgaagaac tcgaagtgct
     3361 cgttttcttc cgtgatccgg tcaaaaccgg cgagcacctg gtcgaggtcc atcggcgcat
     3421 cgacccgatg caggttgaag gcggggacgg tacgcagggt caccgagacc agcacaccga
     3481 gcgacccgac cgcgacccgg gcggccagca acccgtcgga gcccggttcc agctcgcgga
     3541 tctccccgtc ggctcccatg atgcgggcct tctcgatctg tgcggagatg ttcggcaggg
     3601 cacggccggt tccgtgagtc ccggtggaaa cggcgccggc gatcgtctgg cgatcgatgt
     3661 ccccgaggtt cggcattgcc agaccgaggt gatcgagttc acggttgagg tcggcgagaa
     3721 ccgtccccgc cccgacctcg accaggccgg aatcccggtc ggcggagatc accccggaga
     3781 tccgggagat gtcgagcagc aggtcatccg aaagcgcggt cggcgtgaac gaatgtccgg
     3841 agccgaccgt ggtgatctgg cggccggccg cgcccgccgc accgacgacc tcggcaagct
     3901 ccccgacccc gctgacccgg atcaggtcgc gcggccggca gctctgatga ccggcccagt
     3961 tggtccaggc gtgcccgccg cttccgagct tccgggcgtc atagttctca ccgaccatcc
     4021 gctgtacctg cttcctcgct caggtccgaa atcaccggcg gtccgatgcc gagtgaacgg
     4081 gccacgattc ccaccccggc catcacggcc gcggcgacca gacagggcag aaggtatccg
     4141 gccgcaccgg ccaatgcgcc gccgccgatc gcgccgatca tctggccgcc gccccaggcg
     4201 acgttcgatg ctcccgaggc gtaaccctgg ttgaccccgg ccgcggttgc tgtgtcggtg
     4261 accagcgtgc tggccggggt gaaggccatt ccggccccga aggacatcac catcaccact
     4321 gcgaagagca gtgaaagccg gtcgaatatc ccgaggccga ccagggcgag gatcagggca
     4381 cccgccccga tcaggtaggg cagggtgcgg ccgacccggt ccgagatccg cccgatcacg
     4441 ggaccgacca ccgcttcgac gaccgagccg gatgcgaagg cggcggcgat cagaaacggc
     4501 gacgctccga gatcatccat gcggagcggg ccgatcacca ccaccagccc gaaggcgacc
     4561 gaaggtccgg ccagcatcag gactccgctc ggcacgtcgg actggcggat ccggcgccag
     4621 gcctcgccga ggggctggga ctcgacaccg gtcggctcgg gcagggtcac ggcgatcgtg
     4681 atcaggatca gggccgcgac caggaccatg ctgaagaccg gctcg
//
LOCUS       DAOQMF010000034         4689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8639_length_4689_cov_3.129478, whole genome shotgun sequence.
ACCESSION   DAOQMF010000034 DAOQMF010000000
VERSION     DAOQMF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8639_length_4689_cov_3.129478"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..301)
                     /locus_tag="PLV77_01040"
     CDS             complement(41..301)
                     /locus_tag="PLV77_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14464.1"
                     /translation="MERIESRVAKVAKSDKTPKAAAAVVLSGSLLGGADNVQAVTLTA
                     ATLAVAEMARSRFGVKGLRSSGTRSSDKKIGGRIGSVAGRTP"
     gene            complement(443..1279)
                     /locus_tag="PLV77_01045"
     CDS             complement(443..1279)
                     /locus_tag="PLV77_01045"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14465.1"
                     /translation="MTFSPLTKNLLRVAVAITLAFIYIPLLIILIYSTNPNQVLAWPP
                     DGFTLSWFPKAIQDQAARDALWNSVKVGAAATAVALVLGTLASLAVARHKFFGRESIT
                     FLVLIPIALPGIVTGIALNATFTQVFDMKLTLFTVIVAHATFCIVIVFNNAAARLRRM
                     STTLEDASADLGATPWATFRRVTLPNMRSALVAGGLLAFALSFDEIVVTNFTIGAGDQ
                     TLPIYIFNTLFRPNQPGKVYAVAVIAILLSMIPVYIANRLTKEESVTASGAGAGTPIP
                     EP"
     gene            complement(1283..2137)
                     /locus_tag="PLV77_01050"
     CDS             complement(1283..2137)
                     /locus_tag="PLV77_01050"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14466.1"
                     /translation="MGRRLADFFQGRPKLTVAALLAAPLGWLVIGYLGSLGVMLAASF
                     WHLNELSGEVTQGFSLDNFDTILSDDVYRTITLRTIGVAAAVTVTDAILAFPIAFYMA
                     KLARPKVQSILVVAVLLPLWSSYLVKVGAWRLMLSEDGAINWALEPFGLHGPGYGTVA
                     VWLVMSYIWLPYMIIPIYAGLERIPDSLLSASEDLGAKPGRTFRKVIMPLTFPAIVAG
                     SIFTFSLTLGDYITPANVGDKQFIGNVVYSNFVANLPLAAAITVIPIVVMIIYLLIAR
                     RFGAFEHV"
     gene            complement(2213..3409)
                     /locus_tag="PLV77_01055"
     CDS             complement(2213..3409)
                     /locus_tag="PLV77_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC14467.1"
                     /translation="MSYAHRNPARFAWLLVLAMVAVLAVAGCGSSDDDSSSSSDEVTS
                     IGKTEGALNLIAWAGYAEDGSTDKTVDWVTPFEKQTQCQVNTKTASTSDEMVKLMRTG
                     QYDGVSASGDASVRLIEGGDVSPVDMSILSNYADINPSLKDQDYNSKDGQNYGMPHGW
                     GANVLLYNKDAVSPAPDSWGSVFDPAQASKFSGKITAPDNPIYIADAAVYLEATQPDL
                     GITDPYELDEDQFNAAVDLLKQQNKFVGEYWPDATKNISSYTNGDSVIGSSWQYQTSA
                     LQAAGENVDADLPKEGSTGWSDTWMLSSQAEHPNCMLEWMNWMGSPKVQAQVAEWFGE
                     APANLGACDLTSDPKHCDTYHAKDEKYYDQIAFWKTPISDCGDDRGSECKTYDEWVQA
                     WTEIKG"
     gene            complement(3406..4485)
                     /locus_tag="PLV77_01060"
     CDS             complement(3406..4485)
                     /locus_tag="PLV77_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC14468.1"
                     /translation="MSTSSPSTPADQSEAPDVRLTGLTKRYGNFTAVDAIDLEVRQGE
                     FFTMLGPSGSGKTTTLKMIAGFEQPDAGTVELAGQNVSGLAPYARSVNTVFQDYALFP
                     HMTVAANIEYGLSIAKVPKGERAKRRDEALAMVRLEGHGDRKPAELSGGQRQRVALAR
                     AIVNRPRVLLLDEPLGALDLKLREQMQVELKQIQGEVGITFIYVTHDQDEALTMSDRI
                     AVFNEGRIEQLSSPRDLYEHPGNEFVAGFVGVSNAIERDGRKLTIRPEKIRLIGEGEP
                     ADGLVTERGRIAESAYVGMVTRVKVELEQGGAVQVALQNTESEAEPGGRVGQEVVVGW
                     RPEHAVSIQQGERNQSVQLEEEHVS"
BASE COUNT          789 a         1481 c         1570 g          849 t
ORIGIN      
        1 cctcgtgaaa cgggtgccga accccccggg ggagagagga tcagggggtg cggccggcga
       61 ccgatccgat tcggccgccg atcttcttgt cggagctgcg ggtgccactc gagcgcaagc
      121 ctttcacgcc gaagcggctt cgtgccattt cggcaacggc cagggttgcc gcggtcaggg
      181 tgaccgcctg gacgttgtcg gctccgccga ggagagatcc ggagagcacc accgcagctg
      241 ccgccttggg ggtcttgtcc gatttggcga ccttcgccac tctgctttcg atccgttcca
      301 tggcatcaag gtacgaccgg aacgagggcg cgaagttgaa atcctgtgaa tccgccggcg
      361 ccggccgcga gatgcacttg gtcggcgaat atcgccgacc agacgcatcc cggcgccctc
      421 cggttcgacc tcgcaaagcc cgttacggct cgggaatcgg cgttccggct cccgcaccgg
      481 aggcggtgac cgactcctcc ttggtcagcc ggttggcgat gtagaccggg atcatcgaga
      541 gcaggatcgc gatcaccgcc accgcgtaga ccttgcccgg ctggttgggc cggaacaggg
      601 tgttgaagat gtagatcggc aaggtctggt cgccggcccc gatcgtgaag ttggtgacca
      661 cgatctcgtc gaaggagagg gcgaaggcga gcaggccgcc ggcaaccagg gccgagcgca
      721 tgttcggcag cgtcacccgc cggaaggtgg cccagggggt ggcgccgagg tcggcggaag
      781 cgtcctcgag cgtggtcgac atccgccgca accgcgccgc cgcgttgttg aagacgatca
      841 cgatgcagaa ggtcgcgtgg gcgacgatca ccgtgaacag cgtcagcttc atgtcgaaga
      901 cctgggtgaa ggtcgcgttc agggcgatac cggtgacgat gccgggcagg gcgatcggga
      961 tcaggaccag gaaggtgatc gattcccggc cgaagaactt gtggcgggcg accgccagcg
     1021 aagccagggt gcccaggacc aatgcgacgg ccgtggcagc ggcgccgacc ttgaccgagt
     1081 tccagagcgc gtcacgggcg gcctggtcct ggatcgcctt ggggaaccag ctcagggtga
     1141 agccatccgg cggccaggcg agaacctggt tcgggttggt cgagtagatc aggatgatca
     1201 gcagcgggat gtagatgaag gccagggtga tcgccaccgc gaccctgagc aggttctttg
     1261 tcagcgggct gaaggtcatc gctcagacat gctcgaaggc tccgaaccgg cgggcgatca
     1321 ggaggtagat gatcatcacc acgatcggga tcacggtgat cgccgcggcc agcggcagat
     1381 tggcgacaaa attgctgtag acgacgttgc cgatgaactg cttgtcaccc acgttggccg
     1441 gggtgatgta gtccccgagc gttagcgaga aggtgaagat cgagccggcg acgatcgccg
     1501 ggaaggtgag cggcatgatc accttgcgga aggtccggcc cggtttggcc ccgaggtcct
     1561 cggaggcgct gagcagggaa tccgggatcc gctcgaggcc ggcgtagatc gggatgatca
     1621 tgtacggcag ccagatgtag ctcattacca gccagacggc gaccgtgccg tagccggggc
     1681 catgcagtcc gaacggttca agcgcccagt tgatcgcccc gtcctcggag agcatcagcc
     1741 gccaggcgcc gaccttgacg aggtaactgg accagagcgg caggagcact gccacgacga
     1801 gaatcgactg gaccttcggc ctggccagct tggccatgta gaaggcgatc gggaaggcga
     1861 ggatcgcatc ggtgacggtc accgccgcgg cgacgccgat cgtccgcagg gtgatcgtgc
     1921 ggtagacgtc gtcgctgagg atcgtgtcga agttgtcgag gctgaaaccc tgggtgacct
     1981 cgccgctcag ctcgttcaga tgccagaacg aggcagccag catcactccc agcgagccca
     2041 ggtagccgat gaccagccag ccgagcggcg cggcaagaag cgccgcgacg gtcagcttcg
     2101 ggcgcccctg gaagaagtcg gcgagcctcc tgcccaccga cttccgggga gccgggactg
     2161 tggcctcagc ggccaacagt cccgggcact tcttcgtgct tgaaggggtt catcacccct
     2221 tgatttcggt ccacgcctgg acccactcgt cgtacgtctt gcactcgctt ccacggtcat
     2281 cgccgcagtc gctgatcggg gtcttccaga acgcgatctg gtcgtagtac ttctcgtcct
     2341 tcgcgtggta ggtgtcgcag tgcttcggat ccgaggtcag atcacaggca ccgagattgg
     2401 ccggcgcctc gccgaaccac tccgcgacct gggcctgaac cttcggcgaa cccatccagt
     2461 tcatccactc gagcatgcag ttcgggtgtt cggcctggga gctcagcatc caggtgtccg
     2521 accagccggt cgaaccttcc ttcggcaggt ccgcatcgac gttctcgccg gcggcctgca
     2581 gggcactggt ctggtactgc cagctcgagc cgatcaccga gtcaccgttg gtgtaggacg
     2641 agatgttctt ggtcgcatcc ggccagtact cgccgacgaa cttgttctgc tgcttgagca
     2701 gatcgacggc ggcgttgaac tgatcctcgt cgagctcgta cgggtcggtg atcccgagat
     2761 ccggctgggt ggcctcgagg tagacggccg cgtcagcgat gtagatcggg ttgtccggag
     2821 cggtgatctt gccgctgaac ttcgacgcct gggccgggtc aaagaccgaa ccccagctgt
     2881 ccggagccgg actgaccgca tccttgttgt agagcaggac gttggcgccc cagccatgcg
     2941 gcatgccgta gttctggccg tccttcgagt tgtagtcctg gtccttcagg ctcgggttga
     3001 tgtcggcgta attcgagagg atgctcatgt caaccgggct gacgtcgcca ccctcgatca
     3061 ggcgcaccga agcgtcgccg gaggccgaga caccgtcgta ctggccggtc cgcatcagct
     3121 tcaccatctc atccgaagtg ctggccgtct tggtgttgac ctggcactgg gtctgcttct
     3181 cgaacggggt gacccagtcg accgtcttgt cggtcgagcc gtcttcggcg taaccggccc
     3241 aggcgatcag gttgagcgcc ccttcggtct tgccgatcga ggtcacctcg tcactgctac
     3301 tgctgctgtc gtcgtcgctc gagccgcagc cggcgaccgc gagcacggcc accatggcca
     3361 gcacgagcag ccaggcaaac cgggccgggt ttctatgcgc ataactcatg aaacgtgttc
     3421 ctcctccaat tggacggact gatttctctc tccttgctgg atcgaaacgg cgtgctccgg
     3481 ccgccatccg accaccacct cctggccgac tctcccaccg ggctcggctt cagactctgt
     3541 gttctgcaag gcgacctgga ccgcaccgcc ctgctcaagt tcgactttga cccgggtcac
     3601 catgcccaca taggcgctct cggcgatccg gccccgctcg gtgaccaggc cgtcggccgg
     3661 ttcaccttca ccgatcaggc ggatcttctc cggccggatc gtcagtttcc ttccgtcgcg
     3721 ctcgatcgcg tttgacaccc cgacgaaacc ggccacgaac tcgttgcccg ggtgttcgta
     3781 gaggtcgcgg ggcgagctca gctgctcgat ccggccctcg ttgaagacgg cgatccggtc
     3841 gctcatggtc agggcctcgt cctggtcatg ggtcacgtag atgaaggtga tcccgacctc
     3901 accctggatc tgcttcagct cgacctgcat ctgctcgcgc agtttcaggt cgagggcgcc
     3961 gagcggctcg tcgagcagca gcacccgggg acggttgacg atcgcccggg cgagggcgac
     4021 ccgctggcgc tggccgccgg aaagctcggc cggcttgcgg tccccgtggc cttcgagccg
     4081 gaccatcgcg agcgcctcgt cgcggcgttt ggcccgctcg cccttcggca ccttggcgat
     4141 cgagaggccg tactcgatgt tggccgccac ggtcatgtgc ggaaagagcg cgtagtcctg
     4201 gaagaccgtg ttgaccgagc gggcgtacgg ggccaggccg ctgacgttct ggccggccag
     4261 ttcaaccgtg ccggcatcgg gttgctcgaa gccggcgatc atcttcaggg tcgtggtctt
     4321 gccggacccg gacggcccga gcatggtgaa gaactcccct tgccggactt cgagatcgat
     4381 cgcatcgacc gcagtgaaat tcccgtaacg cttggtgaga cccgtcaggc gtacgtcggg
     4441 tgcctcggac tggtcggcgg gtgtggacgg actggacgta ctcactcctc tcgtcgatcg
     4501 cgactgcggg atttgacgga actgatcggg acagatctcc agctaccccg gtcgaatcgg
     4561 actctaaccg ctgggagcgg tcgctgtgaa attcgcctgc cgcccgggtc aggaggcgag
     4621 tcgttcgagc aggtcgagcc ggtcgttgat ctcggcgacg aggccgggcg gcggctcgag
     4681 cccggccat
//
LOCUS       DAOQMF010000035         4683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8654_length_4683_cov_3.832325, whole genome shotgun sequence.
ACCESSION   DAOQMF010000035 DAOQMF010000000
VERSION     DAOQMF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8654_length_4683_cov_3.832325"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2133)
                     /locus_tag="PLV77_01065"
     CDS             complement(<1..2133)
                     /locus_tag="PLV77_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC14469.1"
                     /translation="MNRFAGTALLVLTILLGAVTTASAARDFWNPASSDSSQQFPLLP
                     PPLDGNVQRDDTPNDPGYDRAEPDDEDGATTTNIYDERFDLFGFPSSHTALTLYREGP
                     NALKKQISGFNAAGAWKISRGSPEVAIAVLDTGIKWDREGIRLQVRLNRNELPQPQTA
                     GGSSCAYDCNGDGAFNVADYNDDPRISHTAGPHGSAQIDAEDLITVFSDGTDSDANGY
                     VDDIAGWDFFDNDNNPYDASSYFAASGHGSGRMTEAVERGDDGQGEIGVCPGCQILPI
                     RTWDTFVADGNTVGMGMLYATDAGAKVIVAANGSIYHSAFAEAASRYAYENGVVQTYS
                     GDDLNTANHNYPANYGHAMLIQGVVTDTQGLGTDVGPELRSALCGDLLSPLGVCLGTE
                     LPVGTYFRGANTTQFGGKSSIAMEGATGSENTGKAGGAAGLVISAALQHGITLRPDET
                     RAILEQTAERVTGSALNPLSNVAGLGTADPGADPAAAREDQWTSHFGWGRVNLGEAVR
                     VASDPAATPPEAMIDSPDWYAPINSDSIDLTGLARARAATGTSFSWRLEWGPGQAPTA
                     WNEVASGTGSGSGITDLGSLDMNEVRDAMASYDAPADSGGPTFSTSGANPHDHEFTVR
                     LVVTGSGIATPGIDRRVFTLADDPDLRDGFPKRLGTGGEAPVRYADLDQDGVQELIVP
                     LMDGTVHAFRPDGSELPGWPVETRLMRNA"
     gene            complement(2272..4107)
                     /locus_tag="PLV77_01070"
     CDS             complement(2272..4107)
                     /locus_tag="PLV77_01070"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNC14470.1"
                     /translation="MRVRRLAVMGLLAALAGCLVAAPAQAAPQPKMTAVKAPPKLAVG
                     GRYKVTSIVTNRSGSRVRGPITLTLSKDRKPGAVKLGSRRATASGKGRSRIVTRATLK
                     KSIAVGRYFLVSCFGFPGGSTCAARRVTVIKYVNGARSGGDSLFPEIGNGGYDALHYE
                     ISLDYDPLTNQFNPGTYTKMTARATEYLRQFSLDFEGPEISRIEVNRRRSNFIRRGTK
                     LIITPRIRLDAGRRFTVKVEYSGYVNHHVDPDESLEGWVRACRSGDPGPDNPDCFGSF
                     TVSEPIGAQTWFPNNNVPSDKATFTTTTAVPTEAGADEWTALGTGEFVSKQEGTDGKT
                     TWKWRESLPTSTYLTTGSVCRCVYDVASATDSSNNHTFPLYNAYDNSATPTQVDAINT
                     QLGLQEDIINEFSALFGPYPLKSGGVFAGRTSGIGYVLENQGKIHFPFPGIGNLTLAH
                     EYVHQWFGDAVGPASWSQIWFNEGWAQWGEWFHESPTTPADAFTTEYESTENPERWDI
                     PPGTLNDDPSLLFDEFSTYIRPGMMLEGYRQIVGDAKFSALAKAIQTDYAYSTIDADQ
                     FIATALEVADFGPVQEAALNDYFQQWLFLAGKPSLTPGNFPPEPL"
     gene            complement(4138..>4683)
                     /locus_tag="PLV77_01075"
     CDS             complement(4138..>4683)
                     /locus_tag="PLV77_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HNC14471.1"
                     /translation="GVAPRGRKSVLAGARIKRSTGTGKYFVVSCFRGGWGSTCGATSV
                     RVVPIATGPPGKDGTTGATGSTGATGGTGPIGPIGPTGNTGSTGPTGPTGVTGFTGPT
                     GATGATGATGVTGVTGPIGPTGATGLTGTTGSTGLTGPTGATGLTGSTGLTGPTGSTG
                     LTGSTGLTGSTGFTGPTGPVG"
BASE COUNT          702 a         1501 c         1620 g          860 t
ORIGIN      
        1 ggcgtttcgc atcagccgcg tctcaaccgg ccagcccggc agctcggatc cgtccggtcg
       61 gaacgcgtgg accgtgccgt ccatcagcgg cacgatcagt tcctgaaccc cgtcctggtc
      121 gaggtcggcg taacggaccg gtgcttcgcc gccggtcccg agccgcttcg gaaagccgtc
      181 acgcaggtcc gggtcatcgg ccagggtgaa cacgcgacgg tcgatgccgg gggttgcgat
      241 cccggacccg gtcaccacca gccggaccgt gaactcgtgg tcgtgcgggt tcgcgccgga
      301 agtcgagaag gtcgggccgc cggaatcagc cggtgcgtcg tagctcgcca tggcatcccg
      361 gacctcgttc atgtcgagac tgccgaggtc ggtgataccc gagccgctgc cggtcccgga
      421 agcgacttcg ttccaggcgg tcggagcctg gccgggaccc cattcgaggc gccagctgaa
      481 actggttccg gtcgctgccc gggcgcgggc gaggccggtc agatcgatcg aatccgagtt
      541 gatcggcgcg taccagtcgg gggaatcgat cattgcctcg ggcggagtcg cggcaggatc
      601 ggaggcgacc ctgaccgcct cgcccaggtt gacgcggccc cagccgaagt gggaggtcca
      661 ctggtcttcg cgggctgccg ccgggtcggc gccggggtcc gccgtcccca gcccggccac
      721 gttgctgagc gggttgaggg cggaaccggt cacccgttcg gcggtctgtt ccaggatcgc
      781 cctggtctcg tccggtctca gggtgatccc gtgctggagg gcggcgctga tcacgagccc
      841 tgccgcacca ccggccttgc cggtgttttc cgaaccggtc gcgccttcca tcgcgatcga
      901 actcttgcca ccgaactggg tggtgttcgc cccgcggaag tacgtcccga ccggaagctc
      961 ggtacccagg cagacgccca gcggcgagag cagatcgccg cagagcgcgc tgcggagctc
     1021 cggcccgacg tcggtgccga gaccctgggt gtccgtgacg acgccctgga tcaacatcgc
     1081 atgcccgtag ttggcggggt agttgtggtt ggcggtgttg aggtcgtcac cggagtaggt
     1141 ctggaccacg ccgttttcgt aggcgtacct tgaagcggct tcggcgaagg ccgagtggta
     1201 gatcgaaccg ttggcggcca cgatcacttt cgcgcctgcg tcggtcgcgt agagcatgcc
     1261 catgccgacc gtgtttccgt cggcgacgaa ggtgtcccag gtacgaatcg gcaggatctg
     1321 gcagcccgga cataccccga tctccccctg gccgtcgtcg ccccgctcga ccgcttcggt
     1381 catccggccg gagccgtgac cggacgccgc aaagtagctc gacgcgtcgt aggggttgtt
     1441 gtcgttgtcg aagaagtccc agcccgcgat gtcatcgacg tatccgttcg catccgaatc
     1501 cgttccgtcc gagaagacgg tgatcaggtc ttcggcgtcg atctgggccg agccgtgagg
     1561 gccggcggtg tgcgaaatcc ggggatcgtc gttgtagtcg gcgacgttga aggccccgtc
     1621 gccgttgcag tcgtaggcac agctcgaacc gcccgcagtc tggggctggg gaagctcgtt
     1681 gcgattcagc cggacctgaa gccggattcc ctcccggtcc cacttgatgc cggtgtcgag
     1741 gaccgcgatc gcaacctcgg ggctgccgcg actgatcttc caggcgccgg ccgcgttgaa
     1801 gccggagatc tgcttcttca acgcgttcgg cccttcgcgg tagagagtca gcgccgtgtg
     1861 ggaggaagga aagccgaaga gatcgaagcg ctcgtcgtag atgttcgtgg tcgtcgcgcc
     1921 gtcctcatcg tccggctcgg cgcggtcgta gcccgggtca ttgggggtgt catcgcgctg
     1981 cacgttgccg tcgagcggcg gcggcagaag cgggaactgc tgcgagctgt cactcgatgc
     2041 ggggttccag aagtcacggg cggccgaggc agtggttacc gctcccagca ggatggtcag
     2101 gaccagcagg gctgtgccgg cgaatctgtt cactcccaag ctcccggttt ggtagacgag
     2161 tactggaacc tttatgtgca gatccgccgg ttctcaaaca ggccggagcc cttccttccg
     2221 taaacccggt gcggaaggag gcgggtctgg ccggcgaccc gcgaacggcg cctaaagagg
     2281 ttccggcggg aagttgcccg gagtcagtga aggtttgccc gcgagaaaca gccactgctg
     2341 gaagtagtcg ttcagggccg cttcctgtac cggcccgaag tcggcgacct cgagggcagt
     2401 ggcgatgaac tggtccgcgt cgatcgtcga gtatgcgtag tccgtctgaa tggctttggc
     2461 gagcgcggag aacttcgcat caccgacgat ctggcgatag ccctcgagca tcattcccgg
     2521 ccggatgtag gtcgagaatt cgtcgaacag cagggacgga tcgtcattca gggttccggg
     2581 cgggatgtcc caccgctccg gattctcggt cgattcgtac tcggttgtga aggcgtcggc
     2641 cggtgtggtc ggtgactcgt ggaaccactc gccccactgg gcccagccct cgttgaacca
     2701 gatctggctc cagctggcgg ggccgaccgc atcgccgaac cactggtgga cgtattcgtg
     2761 ggccagggtc aggttgccga tcccggggaa cgggaagtga atcttgccct ggttttccag
     2821 cacgtagccg attccggaag tccggccggc gaagactcct ccggacttca gcgggtaggg
     2881 cccgaacagg gcggagaact cgttgatgat gtcttcctgc aatccgagct gggtgttgat
     2941 cgcatcgacc tgggtcggcg tggccgagtt gtcgtaggcg ttgtagagcg ggaaggtgtg
     3001 gttattcgag ctgtcggtcg ccgaggcgac gtcatagacg cagcgacaga cggagccggt
     3061 ggtcaggtag gtcgaggtcg gcagggattc tcgccacttc caggtcgtct tgccgtcggt
     3121 tccttcctgc ttcgagacga attcccccgt ccccagcgcg gtccattcgt cggcgccggc
     3181 ttccgtcggt accgcggtcg tggtggtgaa ggtcgccttg tcagacggga cgttgttgtt
     3241 cgggaaccag gtctgggcgc cgatcggctc gctcaccgtg aaggagccga aacagtccgg
     3301 gttgtcggga cccggatccc ccgagcgaca ggcgcgaacc cagccttcga gtgattcgtc
     3361 ggggtcgacg tgatggttga cgtaacccga gtactcgacc ttgaccgtga acctgcggcc
     3421 ggcgtccagg cggatccgcg gggtgatgat cagtttcgtg ccccgccgga tgaagttgga
     3481 gcgccttcgg ttgacttcga tccgggagat ctcggggccc tcgaagtcga ggctgaactg
     3541 ccgcaggtat tccgtggccc gggcggtcat cttcgtgtag gtgcccgggt tgaactggtt
     3601 ggtcaagggg tcatagtcga ggctgatctc gtagtggagc gcgtcatagc cgccgttgcc
     3661 gatctcggga aagagcgaat ccccgccgct gcgggcgcca tttacgtact tgatgacggt
     3721 gacccgcctg gcggcgcagg tggatccccc cgggaacccg aaacaggaga cgaggaagta
     3781 cctgccgacg gcgatcgact tcttcagggt ggcgcgcgtg acgatccggc tcctgccttt
     3841 cccgctcgcg gtcgccctgc gggatccaag cttgacggcg cccggcttcc ggtctttcga
     3901 aagggtcaga gtgatcggtc cccggaccct ggatccggaa cggttggtca cgatcgaggt
     3961 caccttgtac cggccgccca ccgccagctt cggcggtgcc tttaccgccg tcattttcgg
     4021 ctgcggtgcg gcctgggccg gcgcggccac aaggcagcca gccagtgcgg ccagtaaccc
     4081 catgactgcc agcctgcgga ccctcatcct tctgccttcc gggtccggcc cggacatcta
     4141 gccgaccggc cctgtgggcc cggtaaaccc ggtcgagccc gtcaaaccgg tcgagcctgt
     4201 caaaccggtc gagccggtcg gtccggtcag accggtcgag cccgtcagcc cggttgcgcc
     4261 ggtcggtccc gtcagacccg tcgagccggt cgtaccggtc aacccggtgg cgccggtcgg
     4321 tccgatcggt ccggtgacac ccgtgacgcc tgtcgccccg gtggcccccg tcgccccggt
     4381 cggtccggtg aagcccgtca ccccggttgg ccctgtggga ccagtcgagc cggtgttccc
     4441 ggtcggcccg atcggtccga tcggcccggt gccgcccgtc gccccggtac ttccggtggc
     4501 gccggtcgtc ccgtccttgc cgggcgggcc ggtggcgatc ggcacgaccc ggacggatgt
     4561 cgcgccacag gtgctacccc atccaccgcg gaaacaggaa acgacgaagt acttcccggt
     4621 gccggtcgag cgcttgatcc gggccccggc cagaacggat tttcttcctc tcggggcgac
     4681 ccc
//
LOCUS       DAOQMF010000036         4682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8657_length_4682_cov_3.573806, whole genome shotgun sequence.
ACCESSION   DAOQMF010000036 DAOQMF010000000
VERSION     DAOQMF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8657_length_4682_cov_3.573806"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLV77_01080"
     CDS             <1..1167
                     /locus_tag="PLV77_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC14472.1"
                     /translation="TMYESAHLISSKTTTAYDEFPMRDEVADFPSHWEMNSYFKDYAD
                     TFDLRRNFRFGTEVTAAEPEDPESESSVWKVTIRNEDGEEESGRYAGVIIANGILSEP
                     SIPTFEGEEEFEGEVLHTASYKRPDIFRDKKVLIVGCGNSGCDIAVDAVHYAKSIEMS
                     LRSGYWFFPKYIFGRPSDTLNEGKALPAFLKTRIDGVFIKFLTGDPARLGFPEPEGRI
                     YETHPVMNTQVLYYAGHGDIHIRKNIKRFTKTGVEFVDGSTGDYDMVMLATGYKLHYP
                     FIDPQYLNWKGNSPDLYLNIFTPKFRNIFVLGMVEATGLGWQGRYNQAHLVAEFIDAG
                     QRNPVRADEMWRRIQGPKPDLFAGYNYRQLDRLPFYVNKDAYRAQIHEHLEIFE"
     gene            1164..2147
                     /locus_tag="PLV77_01085"
     CDS             1164..2147
                     /locus_tag="PLV77_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNC14473.1"
                     /translation="MSLAFLSSLPGLPFGAEDIDSVTLNTSDGMLIVLSVIIGVIMFG
                     IALEMKVDDFRHVMKLPKAMAVGVAAQFICLPAITYLLTVALNFRPSIAMGMILVACC
                     PPGNISNILVYRARGNVALSLSMTALSNFLAIFLMPLNIAFWGGMHPEASQVLKDVDV
                     SAIELLLDIVVLIGIPLTLGLLISSHRPGLAKKVQPIVKKFSLIFLILFIVAALAGNF
                     KVFVDHIALVAVAVFLHDTLALLLGYGIAAGFKVSEPNRRAITFEVGVRNAALGLGIA
                     ISVFDGLGGVAMVAAWWGVWDLIAGLTLSSWWARRPTGDIEESTLEAAESA"
     gene            2161..3114
                     /locus_tag="PLV77_01090"
     CDS             2161..3114
                     /locus_tag="PLV77_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC14474.1"
                     /translation="MKRVFVTGGNGFLGHSVVRGLAEAKDQVELVVSGDLGHPTDERR
                     VEGVDYIVADVTDAASVSGPIGEHGIDTVVHLAAIVNPGNLSTEQEYEVDVTGSKHVF
                     DACLAHDVKRVVVSSSGAAYGYHADNPAWLTEDDPVRGTETFPYSLHKRLVEEQLAAL
                     RESNPELEQVVLRIGTILGETVDNQITDLFKAKRPLKIRGSESPFVFIWDQDVVGVML
                     QAVLGEKTGIFNVAGDGAMTIDEIAAELGKKPRTVPVGVLKAGLGVAHFFNLTPHGAA
                     QTDFLAYRPVLDNRRLKVEFGYTPKYTTREAFHAWRQSQGL"
     gene            3502..3575
                     /locus_tag="PLV77_01095"
     tRNA            3502..3575
                     /locus_tag="PLV77_01095"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3536..3538,aa:Pro,seq:tgg)
     gene            3596..3991
                     /locus_tag="PLV77_01100"
     CDS             3596..3991
                     /locus_tag="PLV77_01100"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNC14475.1"
                     /translation="MPRDSHPSATVHRRIEFSDVDSSGRYHYGTVIRLLEAAEVELLS
                     GLGLLDSIYTSMPRARLEVDYLSTLRFMDEVDVTVEVEDLGRTSVTIGFTVERGGEAC
                     ARGRLVTVFVDQQGTPAPWPAEARTAFGG"
     gene            4256..>4682
                     /locus_tag="PLV77_01105"
     CDS             4256..>4682
                     /locus_tag="PLV77_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HNC14476.1"
                     /translation="MLPSSAGATTVTFNYTGAPESWTVPYGVTSATFNLYGAQGRGFG
                     PGSSAQGGRGGWARATIPVTANSVVTFRVGGRGVANGGGYNGGGSGGYGGGGATDIRI
                     GGDALADRVLVAGGGGGGGFSCLNNNVTVIAVGGDGGGVN"
BASE COUNT          817 a         1520 c         1497 g          848 t
ORIGIN      
        1 acgatgtatg agtcggccca cctgatctcc tcgaagacga ccaccgccta cgacgagttc
       61 ccgatgcgcg acgaggtcgc cgatttcccc agccattggg aaatgaacag ttacttcaag
      121 gattacgcag acaccttcga cctgcgaagg aacttccgtt tcggcaccga ggtgaccgcg
      181 gccgagcccg aggatccgga gtcggaatcg tcggtctgga aggtgacgat ccgaaacgag
      241 gacggggagg aggagagcgg acgctacgcc ggggtgatca tcgccaacgg catcctctcc
      301 gagcccagca tcccgacctt cgagggcgag gaggagttcg agggcgaggt gctccacacc
      361 gcctcctaca agcgccccga catcttccgc gacaagaagg tgctgatcgt gggctgcggc
      421 aactcgggct gcgacatcgc cgttgacgcg gtccattacg cgaagtcgat cgagatgagt
      481 cttcgctccg gctactggtt cttccccaag tacatcttcg gccggccctc cgacaccctg
      541 aacgagggca aggccctgcc ggcctttctc aagacccgaa tcgacggggt cttcatcaag
      601 ttcctgaccg gtgacccggc ccggctcggc ttccccgagc cggaggggcg gatctacgag
      661 acccacccgg tgatgaacac ccaggtgctc tactacgccg gccacggtga catccacatc
      721 cgcaagaaca tcaagcgctt cacgaagacc ggagtcgaat tcgtggacgg ttcaaccggt
      781 gactacgaca tggtcatgct ggccaccggc tacaagctcc actacccgtt catcgacccg
      841 cagtacctga actggaaggg caacagtccc gacctctacc tgaacatctt caccccgaag
      901 ttccgcaaca tcttcgtgct cgggatggtc gaggcgaccg gtcttggctg gcagggtcgc
      961 tacaaccagg cccacctggt cgccgagttc attgacgccg gccagcggaa cccggtccgg
     1021 gccgacgaga tgtggcgtcg gatccagggc ccgaagccgg acctcttcgc cggctacaac
     1081 tatcgccagc tcgatcggct tccgttctat gtgaacaagg acgcctaccg ggcccagatc
     1141 cacgagcacc tggagatctt cgaatgagtc tggccttcct ttcctccctg ccgggcctgc
     1201 ctttcggggc ggaagacatc gacagcgtga ccctgaacac cagcgacggc atgctgatcg
     1261 tgctcagcgt gatcatcggg gtgatcatgt tcggcatcgc cctggagatg aaggtcgatg
     1321 acttccgtca tgtgatgaag cttccgaagg cgatggcggt cggcgtggcg gcccagttca
     1381 tctgcctgcc ggcgatcacc tacctgctca cggtggccct caacttccgg ccctcgatcg
     1441 ccatggggat gatcctggtc gcctgttgtc cgccgggaaa catctcgaac atcctcgtgt
     1501 accgggccag aggaaacgtg gccctgtcgc tttcgatgac cgccctttcg aacttcctcg
     1561 cgatcttcct gatgccgctc aacatcgcct tctggggagg catgcatccg gaggcatccc
     1621 aggtcctcaa ggacgttgac gtcagcgcga tcgaactgct gctcgacatc gtcgtgctga
     1681 tcggaattcc gctgacgctc ggcctgctga tctccagcca ccgcccgggc ctggccaaga
     1741 aggtccagcc gatcgtcaag aagttcagtc tgatcttcct gatccttttc atcgtcgcgg
     1801 ccctggccgg caacttcaag gtcttcgtcg accacatcgc cctggtcgcg gtcgccgtct
     1861 tccttcacga cacgctcgcg ctgcttctcg ggtacgggat cgcggccggc ttcaaggttt
     1921 cagagccgaa ccgaagagcg atcacattcg aggtcggggt gcgcaatgcg gcgctcggtc
     1981 ttggcatcgc gatcagcgtc ttcgacggtc tcggaggcgt ggcgatggtg gccgcctggt
     2041 ggggggtctg ggacctgatc gccggcctga ccctctccag ctggtgggcg aggagaccga
     2101 ccggggacat agaagagagc acgctggagg cggccgaaag tgcctgaagc cggcggcggg
     2161 atgaagcgcg tattcgtcac cggcggcaac ggtttccttg gccattcggt ggtgcggggc
     2221 ctggctgagg cgaaggacca ggtcgagctc gtcgtctcgg gtgatctcgg ccacccgacc
     2281 gacgagcggc gggtcgaagg ggtcgattac atagtcgccg acgttaccga tgccgcctcg
     2341 gtttccgggc cgatcggcga gcacgggatc gacacggtcg tccacctcgc ggcgatcgtc
     2401 aatcccggca acctctcgac cgaacaggaa tatgaggtcg atgtgaccgg gtcgaagcat
     2461 gtgtttgacg catgcctcgc tcacgatgtg aagcgtgtgg tggtctcttc cagcggcgcg
     2521 gcctacggct accacgccga caacccggcc tggctgaccg aggacgatcc ggtccgcggc
     2581 accgaaacct tcccctacag cctgcacaag cgtctggtcg aggagcagct ggcggcgctt
     2641 cgggaatcca atccggagct cgagcaagtg gtgctccgga tcggcacgat ccttggcgag
     2701 acggtggaca accagatcac cgacctcttc aaggcgaaac gaccgctgaa gatccgcggc
     2761 tccgagtcac cgttcgtctt catctgggac caggacgtgg tcggggtgat gttgcaggcg
     2821 gtgctgggcg agaagaccgg catcttcaac gtcgcgggtg acggcgccat gacgatcgac
     2881 gagatcgccg ccgagctcgg caagaagccc cgcacggttc cggtcggggt cctgaaggcc
     2941 ggtctcggcg tcgcccactt cttcaacctg accccgcacg gcgcggccca gaccgacttc
     3001 ctcgcctacc gcccggtcct cgacaaccgc cgcctgaagg ttgagttcgg ctacaccccg
     3061 aagtacacaa cccgcgaggc cttccacgcc tggcgccagt cccagggact ctgaacctcc
     3121 cacgccgccg gcccggcccc gccagccgcc attccctggg tctctccgag tccggcccgg
     3181 gccccgctag ccgccattcc cggggtctct ccaagtccgg cccgggcccc ggccccgccc
     3241 gttgagtccc ggggtctctg gccggccccg gacccagacc gccaggttcc actttgcgcc
     3301 acatatcgca cccaactccg agctcgatcg ggagcggccg atcgagatcg gagttgggag
     3361 cgctatgtac caacttctcc cttctcgatt ccacgagcaa cggggtcctt caggtggggt
     3421 caagcgacac cgactgcgcg tcaggcttcg ggcatggatg gatcgcatcc gaggcgagtg
     3481 gaagtagaat cgtcaccgct tcggggtgta gcgaagcctg gtatcgcgtt cgctttgggg
     3541 gcgaaagatc cccggttcga atccgggcac cccgattggt cgaacacccg cgcccatgcc
     3601 tcgagattcc cacccctcag ccaccgttca tcgccggatc gagttttccg atgttgattc
     3661 ttcgggccgg taccactacg ggaccgtgat ccggttgctg gaggcggccg aggtcgagct
     3721 gctttccggg ctgggtttgc tcgattcgat ctacacctcg atgccgcggg cccggctgga
     3781 ggtcgactat ctctcgaccc tgcggttcat ggacgaggta gacgtgacgg tcgaggtcga
     3841 ggatctcggc cgcacctcgg tcaccatcgg attcacggta gaacgcgggg gagaggcctg
     3901 cgcccgaggc cgcctggtca ccgttttcgt cgaccagcag ggcacaccgg ccccctggcc
     3961 cgccgaagcc cgcaccgcct tcggcggctg aaagccgcgg gggcgcaagc acccagggga
     4021 tcgatcactt cgaccggaca ttcgacgagg cgggcgtggc aagaacttgt ggcccgcttg
     4081 cgagcgtttg acccggccgc cccggtttct cgatgtttct gatcgttgtt cgatttcccg
     4141 ggcgaagccc gataggatcg agatgaatcc gccttcgggc aaaaacggga atcgggggaa
     4201 gttgagacgt ttaacgggat cgttggtggg gggacttgcg ttggtgtgcg ccttcgtgct
     4261 gccgtcgtcg gcaggggcga cgaccgtgac cttcaactac accggtgccc cggagtcctg
     4321 gacggttcct tacggcgtca cctcggcgac cttcaatctg tacggagccc aggggagagg
     4381 tttcggaccc ggatcatctg ctcagggagg tcgaggcggc tgggccaggg ccacgatacc
     4441 cgtgactgca aactcggtgg tgaccttccg ggtgggtggc cggggcgtag ccaacggcgg
     4501 tggatacaac gggggaggca gcggtggcta cggtggcggc ggggccaccg acatacgcat
     4561 tggcggagac gccttggccg accgtgtact cgtggccggc ggaggcgggg gcggaggctt
     4621 cagctgcctg aacaacaacg tcaccgtaat cgccgtgggc ggcgacggcg gtggtgtgaa
     4681 cg
//
LOCUS       DAOQMF010000037         4666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8711_length_4666_cov_3.080677, whole genome shotgun sequence.
ACCESSION   DAOQMF010000037 DAOQMF010000000
VERSION     DAOQMF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8711_length_4666_cov_3.080677"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLV77_01110"
     CDS             complement(<1..357)
                     /locus_tag="PLV77_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14477.1"
                     /translation="MFVQKEKKSSRVATRAADALDLMIEFATLGEYGLEYPEPVRSGS
                     RRNTTPDVRMERIARILEGSQPGDATGGSGFVDQERSAGHRRGSTPQKHRHGRRPCRP
                     GRTRVDLPGWGDRPGHE"
     gene            complement(558..3878)
                     /locus_tag="PLV77_01115"
     CDS             complement(558..3878)
                     /locus_tag="PLV77_01115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNC14478.1"
                     /translation="MSSYVELHCHSSFSFLDGASSPLELAEKAAELGYPALALTDHDG
                     VWGSMEFAVACKGAGIRAITGTELTVEEGGRLFHLTLLVENETGYHNLCRLLTASHAH
                     TRDGRDRRVGQPRVPLGDLAGRAEGLICLTGCARQGLIPAAWADGDLARGETLARRLR
                     RWFTPENLRVEIQRPYWRHDHARNRWLAGLARSLELPLVATGNVHSHERERARLQDAL
                     VAVRLGSNLADSEPQRRGNSTSTLASPGQMAYRFPDYPEAVSETVRVAERLVFDLERD
                     LGYRYPQQGDPKTDRDLAALCQTRIDERYRGSRQEPEARARLENELKIIRGLGLSGFF
                     LLHHDLLELAREVAVEVRGTSSARGVLPPGRGRGSSVSSIVCYLTGLSHIDPVKADLF
                     PGRFLNEEVTTAPDIDLDFPRDIRERLIPRIHQRYGRDRSALVAAFPTYRSRGAIRDF
                     GKVLGIPDTEIERAARAVDFHSGSDDFQRDLTQAIGRERAHSAAWKHLALLSRQARSL
                     PRHASQHSGGMVIATRPLVEICPVVPAAMEQRQIVQWDKDSCQDAGFLKIDLLGLGML
                     SAVERTIDEIHRIRGETVDLSRIDLDDRAVFGMIKRAETTGVFQIESRAQMQMLPRMR
                     PDNIDDLTIQVALIRPGPIQGGAVHPYIERRRRLREDPDYEIPFAHELLRPVLGETHG
                     VIIFQEQVIEVAMHVAGFSSSEAESLRRAMSRKRSRRSLEAHHQRFIEGAMKSGVSRE
                     IADGIFEQIIGFSGFGFPKAHSAAFGLLAYQSAWLKHHYGPEYLASLLNEQPMGFYPP
                     DSLVQEARRTGVEVLPPDVNHSRVECVTEWPGSGGESSPRAESGPEGALARDEAATAR
                     RQQPAVRIGLGYVKGINESEMEKLVAERDRGGSFGDLGDLASRLPLARSDLEQLAWAG
                     ALRSLPRGERVAGLWSVGLTANRLPRAGGRQLALPFSQEETPELPEPKDWARVQAEYG
                     SIGMTLEGHPMELARRQLAESVLSTTDAASRPDRSWVEVAGLQVARQRPETAHGVIFI
                     LIEDEHGTLNLILPPPVAKRHRQVIRTATLIKANGRIETGQGVVNLVVRGLVEVHPPD
                     PRIKASAPVGINFGRRGR"
     gene            complement(3963..4247)
                     /locus_tag="PLV77_01120"
     CDS             complement(3963..4247)
                     /locus_tag="PLV77_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14479.1"
                     /translation="MSASMRRATMVTSGTRPTRLYEPRPVEVASDAAGAPVQINDVDV
                     VQVREEWIVEDGWWTAAPISRWYFEVVLIDGRDLAVFRAFPSGQWFAQRA"
     gene            complement(4250..>4666)
                     /locus_tag="PLV77_01125"
     CDS             complement(4250..>4666)
                     /locus_tag="PLV77_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14480.1"
                     /translation="LTILCDRLAGRMRAAGLSLRSLSVEARLLGGGSWSHRASPRRPT
                     TSSPVLRMLLFPALEQLSRPADRLGLRALETAPQAADQLEVIHRPGETRRRRLGEAAR
                     QVRAAVGESALMRILETEPESRLPERRMLLTPYSTE"
BASE COUNT          725 a         1560 c         1612 g          769 t
ORIGIN      
        1 ttcatgcccg gggcgatcac cccagccggg gaggtcgacc cgggtccgcc cgggccggca
       61 tgggcgtcgg ccgtgacgat gcttctgggg ggtggagcca cggcggtggc cggccgaacg
      121 ctcctggtcg acgaatccgg agcctccggt ggcgtcgcct ggctggctgc cctcgaggat
      181 gcgtgcgatg cgctccatgc ggacgtcggg ggtggtgttc ctgcggctgc ccgacctgac
      241 cggttccggg tactcgagcc cgtattcgcc gagggtggcg aactcgatca tcaggtcgag
      301 agcatcagcc gcccgggttg ctacccggct gctcttcttc tctttctgca cgaacatagg
      361 ttcgtaattt acgggcccgg tcggacacga acacatgttt gcaattaacc gtgcagaatc
      421 gttcggaaac cgtttggcag agccaagtgc aaaccgtggt ttcgggctgt cggtcggccg
      481 cctgcacgaa caggcgtttg tttttcgttc gcattttgcg gcatcccgaa acgctcgttc
      541 gggcccgttt ccggcgctca ccggccgcgc cgcccgaagt tgatcccgac cggtgcgctg
      601 gccttgatcc gggggtcggg cggatgaact tcaaccaggc cgcgcacgac caggttgacc
      661 accccctggc cggtctcgat gcgtccgttt gccttgatca gggttgcggt gcggatcacc
      721 tggcgatgtc gtttggccac cggcggcggc aggatcaggt tgagggtgcc gtgttcgtcc
      781 tcgatcagga tgaagatcac tccgtgggcg gtttccggtc gctggcgggc aacctggaga
      841 ccggccacct cgacccagct gcgatccggc cgggaggcgg cgtcggtcgt ggacagcacg
      901 ctttcggcca gttgacgacg ggccagctcc atcggatgtc cctccagggt catgccgatc
      961 gagccgtatt cggcctggac ccgcgcccag tccttcggtt ccgggagttc cggggtctcc
     1021 tcctgactga acggcagcgc gagttgccgc ccgccggccc gcggcaaccg gttcgccgtg
     1081 agtccgacgc tccacagtcc ggccacccgc tcaccacggg gcagggacct gagtgccccg
     1141 gcccaggcca gctgctcgag atcgcttcgc gcgagtggca ggcgggaggc gaggtcgccg
     1201 aggtcgccga agctgccacc gcggtcacgc tcggcgacca gcttctccat ctcgctctcg
     1261 ttgatcccct tcacataacc gagaccgatc ctcacggcgg gctgctgccg ccgggctgtt
     1321 gcggcttcgt cccgggccag ggcaccttcc gggccggact ctgcccgcgg agagctttcc
     1381 ccgcccgacc ccggccattc ggtgacgcat tcgacccggc tgtggttcac gtcgggcggc
     1441 aaaacctcga ccccggtgcg ccgggcttcc tggaccagtg aatccggcgg gtagaagccc
     1501 atcggctgct cgttcagaag cgaagccagg tactccggtc cgtagtggtg cttgagccag
     1561 gccgactggt aggccagcag gccgaaggcg gcggaatgag ccttgggaaa gccgaagccg
     1621 gagaagccga tgatctgctc gaagattccg tcggcgatct cgcggctgac gccgctcttc
     1681 atcgcgccct cgatgaaacg ctgatgatgg gcctccagcg agcggcgtga gcgcttgcgg
     1741 ctcatcgccc ttctaagcga ctcggcctct gaggaactga agccggccac atgcatcgct
     1801 acctcgatca cctgttcctg gaagatgatc accccgtggg tctcgccgag caccggcctg
     1861 agcagctcat gggcgaacgg gatctcgtaa tccgggtctt ccctcagcct tcgccgcctc
     1921 tctatatatg ggtgaacggc gccgccctga attggcccgg gacggatcag tgccacctgg
     1981 atggtcaggt catcgatgtt gtccgggcgc atccggggca gcatctgcat ctgggcccgg
     2041 ctttcaatct ggaagacacc ggtggtctcg gcccgcttga tcattccgaa gaccgcccgg
     2101 tcatcgaggt cgatccggct caggtccacg gtttcacccc ggatccggtg gatctcgtcg
     2161 atcgtccgct cgaccgccga gagcatgccc agcccgagca ggtcgatctt gaggaaaccc
     2221 gcgtcctggc aggaatcctt gtcccactgg acgatctggc gctgctccat cgccgccggt
     2281 acgaccgggc agatctcgac cagcggccgg gtcgcgatca ccatcccgcc cgaatgctgt
     2341 gaagcgtgac ggggcaggct gcgggcctgg cgggaaagca gggcgagatg cttccaggcc
     2401 gccgagtggg ccctttcgcg gccaatcgcc tgggtcaggt cacgctgaaa atcgtcggag
     2461 ccgctgtgaa agtccaccgc ccgcgcggct cgttcgattt ccgtgtcggg gatgccgagg
     2521 accttgccga agtcgcggat cgccccgcgg gagcgatagg tgggaaaggc ggcgaccagg
     2581 gcggagcggt cccggccgta cctttgatgg atgcggggga tcagccgctc cctgatgtcc
     2641 cgggggaagt cgagatcaat gtcaggggcc gtggtcacct cttcgttgag gaaacggccg
     2701 gggaagaggt cggccttgac cgggtcgatg tgggagaggc cggtcaggta acagacgatc
     2761 gagctgaccg aggaaccgcg tccccggccc ggcggcagca cgccccgggc ggaggaggtg
     2821 ccacgcacct cgaccgccac ctcgcgggcc agctccagca ggtcgtgatg gagcaggaag
     2881 aagccggaga gcccgaggcc gcggatgatc ttcagctcgt tctcgagacg ggcccgggcc
     2941 tccggctcct gccgggaacc ccggtagcgc tcgtcgatcc gggtctggca gagcgccgcg
     3001 agatcccggt cggtcttcgg gtcgccctgc tgcgggtagc ggtaaccgag gtcgcgttcg
     3061 agatcgaaaa cgagtcgctc cgcgacccgg accgtctcgc tgaccgcctc cgggtaatcc
     3121 ggaaagcggt acgccatctg accgggcgag gcgagggtgg aggtggagtt gccccgccgc
     3181 tgaggctcgg agtcggccag gttcgagccc agccggacgg cgaccagggc gtcctgcagt
     3241 cgggcccgtt cccgttcgtg actgtgcacg ttgccggtcg caaccagcgg cagctccagg
     3301 ctgcgggcca gcccggccag ccagcggttg cgggcatggt catgccgcca gtagggccgc
     3361 tggatttcga cccgcaggtt ttccggagtg aaccagcgac ggagccgcct ggcgagggtt
     3421 tcgccccggg ccaggtcacc atcggcccag gccgccggga tcagcccctg cctcgcgcag
     3481 ccggtcaggc agatcagccc ttcggcccgg ccggccagat caccaagcgg cacccggggc
     3541 tggccgaccc gccggtccct tccgtcccgg gtatgggcgt gggaggcggt gaggagccgg
     3601 cagaggttgt ggtagccggt ctcgttttca accagcaggg tcaggtggaa gagccggccg
     3661 ccttcctcga cggtcaactc ggttccggtg atcgcccgga tccccgcgcc cttgcaggcg
     3721 accgcaaact ccattgaccc ccagaccccg tcgtgatcgg tcagggccag ggccgggtaa
     3781 cccagttcgg ccgccttctc tgccagttcc agcggcgaag aagccccgtc cagaaaggag
     3841 aaagacgaat ggcaatgcag ctcgacatac gacgacatgt catcctcggg cccggcgagg
     3901 tcgggagggg caccgagatg gcaaaagtgg ccatatagcg ctctcttttg ccatctcgtt
     3961 cctcaggccc gctgggcgaa ccactggccg gaggggaagg cacggaagac ggccaggtca
     4021 cggccgtcga tcagcaccac ctcgaaatac cagcggctga tcggggcggc ggtccaccag
     4081 ccgtcctcga cgatccattc ctcccggacc tggaccacgt ccacgtcgtt gatctggacc
     4141 ggagcccccg ccgcgtcgct ggccacctcg accggtcgcg gctcgtaaag ccgggtcggc
     4201 cgggtgcccg aagtgaccat ggtcgctctt ctcatcgaag cgctcaccgc tactcggtcg
     4261 agtagggggt caggagcatg cgccgctcgg gcaggcggga ctcgggctcg gtttccagga
     4321 tccgcatcag ggcgctttcc ccgaccgcgg cgcgaacctg acgggccgct tcaccgagcc
     4381 ggcggcgacg ggtttcgccg ggacggtgga tgacctcgag ctgatcggcc gcctggggag
     4441 cggtctccag cgcccgcagg ccgagccggt cggccgggcg ggaaagctgc tcgagagcgg
     4501 ggaagaggag catccgcaaa accggcgagg aggtcgtggg gcggcggggc gaggcccggt
     4561 gcgaccagct gccgccgccg agcagccggg cctcgaccga aagcgacctc aggctgaggc
     4621 ccgccgcccg catcctcccg gccaggcgat cgcaaagaat ggtcag
//
LOCUS       DAOQMF010000038         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8849_length_4619_cov_3.762708, whole genome shotgun sequence.
ACCESSION   DAOQMF010000038 DAOQMF010000000
VERSION     DAOQMF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8849_length_4619_cov_3.762708"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLV77_01130"
     CDS             <1..620
                     /locus_tag="PLV77_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14481.1"
                     /translation="ATGSRDRIQDPEEMPLRSEERFPIRRSVTLIVAAFTLTGSAFAV
                     SGCGGSGEEPKQWPNDAGYPFDEVKLAADGVPRTAKLTADQKAEILRIARNDPLVKEL
                     LEGHEVEWRKPYPWSRPNMELLGGGVDVHFAEPVDLSGATIPAIENIEELYGKQGEEG
                     VILEEGEPRVEPEIGTTRSPYTDVRALSLLVDLEQGRVAQVLQIG"
     gene            622..1497
                     /locus_tag="PLV77_01135"
     CDS             622..1497
                     /locus_tag="PLV77_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNC14482.1"
                     /translation="MSFSQSVSEAVDPDVVSCLPGGGGDINRGWRIETAEGDRFFAKS
                     RPGGSVAEFVNEAAGLRWLGEVGALPVPEVIAVVEDETEPGLILDWVEPGPRADEAEL
                     GRGLALIHQAGADGFDRLAPGATGNEIRFGEAAMPLNPVPAGEGFAGVYAARLRHLGG
                     EAYERGALGSSDRDVIEQVADRADGLAGPVEPPARVHGDLWSGNLLWSEGRPWLIDPA
                     AHGGHRELDLAMLELFGSPGPAFYSAYEEVAPLADGYRERIGFWQLQPLLVHAVLFGG
                     GYGRSAAVTARACLG"
     gene            1608..2441
                     /locus_tag="PLV77_01140"
     CDS             1608..2441
                     /locus_tag="PLV77_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNC14483.1"
                     /translation="MNKWRYLLVSVLAALVAGLTIPAASSAWAPAASATVHPGVQTVT
                     EGAQCTSNFVFQNGSDVYLGQAAHCSGTGEATDTDGCQAGALPIGTAVEVTGASQPGT
                     LAYSSWNSMQAKGTTDASTCAFNDFALVRINPADVAGVNPSVPGYGGPTGLGAPGGLG
                     STVYSWGNSSLRQGIRQLSPKQGIVLSNDASGWSHNVYTLTPGIPGDSGSGFLNNSGQ
                     AFGTLSTLQLAPTVGANGVGDLAKELTWLKANEPAFAGIQLVNGTEPFKPNLVGAILG
                     G"
     gene            complement(2493..3584)
                     /locus_tag="PLV77_01145"
     CDS             complement(2493..3584)
                     /locus_tag="PLV77_01145"
                     /inference="COORDINATES: protein motif:HMM:NF022470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HNC14484.1"
                     /translation="MSDSYDFAVRRDDMRETSVLETPGADREIDEGQILLEVDHFSLT
                     ANNVTYGAMGEAMNYWDFYPAEDDSLGRIPAWGYADVVVSKAEGIEEGARVFGYLPMS
                     THAVLEPGRIRESGFTDITGFRSGLPAVYNEYRLITGEKDDERERLVAIFMPLYGTSW
                     LLADWLNEVDFAGASQLVASSASSKTALGLGHALQVDFDNPIETVGLTSPGNLEFTTG
                     TGHWDAVVTYDDLDSIDADTPTIYVDFGGNAKLRHRIHSHFGDRLVASVAVGIADWES
                     MVPEEGDSLPGPQPQFFFAPDRVVKRNADWGQDGLGERLAKSQDRFTDRSREWMEVQT
                     GSGPDAIRESLLALMDGKVPPDRGISVNP"
     gene            3746..>4619
                     /locus_tag="PLV77_01150"
     CDS             3746..>4619
                     /locus_tag="PLV77_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC14485.1"
                     /translation="MEDAGLGPVRTFSAPEAMGIRFHEVRAKSALNRVPEGPYGFGWT
                     VNPYRGCSHACVYCFARPTHTYLGFNAGRDFDREIVVKVNAPEVVRAELARPSWKREL
                     VALGTNTDPYQWVESRYRLTRGVLEALDEADTPVSVLTKSPLVLSDLPIFERMNQARP
                     TGVNLSVPTVDEKAWRATEPHTPSPQARLEAVAELRRRGIPSGILIAPIMPGINDRPD
                     QIEPILQAASDADADFVTPIALNLRKETRTVFFDWLEAERPELLPEYGRLFDGRSRLP
                     KIEQERVIAAARGRY"
BASE COUNT          800 a         1532 c         1525 g          762 t
ORIGIN      
        1 ctgccaccgg cagcagagac cggatccagg acccagagga aatgccattg cgatcagaag
       61 agaggttccc catccgccgg agcgtcaccc tgatcgtggc cgccttcacc ctgacgggat
      121 cggcattcgc ggtttcgggt tgcggcggca gcggagaaga accgaaacag tggccgaacg
      181 acgcgggcta tccgtttgac gaagtgaagc tggctgcaga cggtgtaccc cgaaccgcga
      241 aactcactgc ggaccagaaa gccgagatcc tgagaatcgc caggaacgat ccgctcgtga
      301 aggaactact ggaaggccat gaggtggagt ggcgcaagcc ctacccctgg tcccgtccga
      361 acatggaact cctgggagga ggagtcgatg tccattttgc cgagccggtc gacctgtctg
      421 gcgccacgat tcccgccatc gagaacatcg aggagctgta cggcaagcaa ggcgaggaag
      481 gagtgatcct cgaagaaggc gagcctcgcg tagagccgga gatcggaact acccggtccc
      541 cttacacgga cgtgcgtgcc ttgagcctgc tggtcgacct cgagcagggc cgggtcgccc
      601 aggtgctcca gattggctga gatgtccttc tctcagtcgg tatcggaagc cgtcgatccg
      661 gacgtcgtct cctgcctgcc cggtgggggc ggggacatca atcgggggtg gcggatcgag
      721 accgctgaag gagaccggtt ctttgccaag agccggcccg gcgggtcggt ggcggagttt
      781 gtgaacgagg cggccgggct tcgttggctc ggtgaggtcg gggcgctgcc ggtgcctgaa
      841 gtgatcgcgg ttgtcgaaga cgagaccgag ccggggttga tcctcgactg ggtcgagccc
      901 gggccacggg ctgacgaggc cgagcttggt cgcgggctgg cgctcatcca tcaggcaggt
      961 gccgatggat tcgaccggtt ggcaccgggg gccaccggga acgagatccg gttcggtgag
     1021 gccgcgatgc cgctgaatcc agtcccggcc ggggaagggt tcgcgggggt ctatgcggcc
     1081 cgcctgcggc accttggcgg cgaggcgtac gagcgcggcg cgctcgggtc ctcggatcgc
     1141 gatgtgatcg agcaggtcgc cgaccgggcc gacggtctgg ccgggccggt cgaacccccg
     1201 gcccgggtcc acggcgacct ctggtccggc aacctgctct ggagcgaggg ccggccctgg
     1261 ctgatcgacc cggccgctca cggcggccac cgcgaactcg acctggcgat gctcgagctt
     1321 ttcggcagtc ccggacccgc tttctattcg gcctacgagg aagtcgcgcc cctggccgac
     1381 ggataccggg aacggatcgg cttctggcag ctccagccgc tgctggttca tgcggtcctc
     1441 ttcggtggtg gttacggccg aagcgctgcg gtcaccgccc gggcctgtct cggctgaggg
     1501 aatcgaagtg cagttggtcg gcgatattcg ccgaccaacc gcatctcacg gaccctttat
     1561 gagaaacgaa gtgtttatgg ctaccccgat ggggtagtag atctcgcatg aacaaatgga
     1621 gatacctgct tgtttctgtc cttgcggcgc ttgtggccgg cctgaccatc ccggcggcct
     1681 cgtcggcctg ggcaccggct gcttcggcga ccgtccaccc cggcgtccag accgtgaccg
     1741 agggcgccca gtgcacctcg aacttcgtct tccagaacgg gtccgacgtc tacctcggcc
     1801 aggccgctca ctgttccggc accggcgagg cgaccgacac cgatggctgt caggccggcg
     1861 ccctgccgat cggcaccgcg gtcgaggtga ccggggccag ccagccgggc accctcgcct
     1921 acagctcgtg gaattcgatg caggccaaag gcacgaccga cgcctcgacc tgcgccttca
     1981 atgacttcgc gctggtcagg atcaatccgg ccgacgtcgc cggcgtcaac ccctcggtcc
     2041 ccggctacgg cggaccgacc gggcttggtg cgccgggcgg acttggctcg accgtctact
     2101 cctggggcaa ctcgtcgctc cgccagggca tccgccagct cagcccgaag cagggcatcg
     2161 ttctcagcaa tgacgccagc ggctggagcc acaacgtcta caccctgacc cccggcatcc
     2221 ccggtgactc cggcagcggc ttcctgaaca acagcggtca ggccttcggc acgctcagca
     2281 ccctgcagct cgcacccacg gtgggcgcga acggagtcgg tgacctcgcg aaggaactga
     2341 cctggctcaa ggcaaacgag ccggccttcg ccggcattca gctggtcaac ggaaccgagc
     2401 ccttcaagcc gaacctggtc ggcgcgatcc tcggcggctg agctggaacc cgtggccaag
     2461 actccgtcct cgggacgtct tggccacggg ttctagggat tgaccgagat gcctcgatcc
     2521 gggggtacct tgccgtccat cagggcgagc agggactccc ggatcgcatc cggtccgctt
     2581 cccgtctgaa cttccatcca ctcgcggctg cggtcggtga aacggtcctg gcttttggcc
     2641 aggcgctcgc cgaggccgtc ctgcccccag tcggcgttgc gcttgacgac ccggtcgggc
     2701 gcgaagaaga actgcggctg gggcccgggc agagaatcgc cttcttccgg aaccatcgat
     2761 tcccagtcag cgatcccgac cgcaacgctg gccacgagcc ggtcaccgaa gtgactgtga
     2821 atccggtgcc tgagcttcgc gttgccgccg aaatcgacgt agatggtcgg cgtgtccgcg
     2881 tcgatgctgt cgaggtcgtc ataggtgacg accgcatccc agtgcccggt gcccgtggtg
     2941 aactcgagat tgccgggcga ggtcaggccc accgtctcga tcggattgtc gaaatcgacc
     3001 tgcagcgcgt ggccgagacc gagcgctgtc ttgcttgaag cgctggaggc aaccagctgg
     3061 gaggcgccgg cgaaatcgac ttcattcagc cagtcggcca gaagccacga ggttccgtag
     3121 agaggcatga agatcgcaac cagccgctcc cgttcatcgt ccttctcccc ggtgatcagg
     3181 cggtattcgt tgtagaccgc cgggagcccg gagcggaaac cggtgatatc ggtgaaaccc
     3241 gactcccgga tccggcccgg ctcgaggacg gcgtgagtcg acatcggcag gtacccgaag
     3301 acccgggcac cttcctcgat tccctcggcc ttcgagacca ccacgtcggc atatccccag
     3361 gccgggatcc ggcccagtga atcatcctcg gccggataga aatcccagta gttcatcgcc
     3421 tcgcccatcg caccgtaggt gacgttgttg gcggtcagcg agaagtggtc gacctcgagc
     3481 aggatctggc cttcgtcgat ctcccggtcg gctcccggag tctcgagaac cgaggtttcc
     3541 cgcatgtcgt cgcggcggac tgcaaagtca taagagtcgg acatgtcggt gagactatcg
     3601 atttgactga aacacttgca gagggtgttg catcagcgaa ccgggtcgcc ggatatgcga
     3661 acatatgttc gtgcgatggg acgggcaacg ggtaaccgcc gacgggcgcc cggcgaaggc
     3721 cggcccggca atggccttgc cggggatgga agacgccggg ctcggtccgg tacgcacctt
     3781 ctcggctccg gaagcgatgg ggatcaggtt ccacgaagtc cgggccaagt cggccctcaa
     3841 ccgggtgccg gaaggccctt acggcttcgg ctggacagtc aacccgtacc gcggctgcag
     3901 ccacgcctgc gtctactgct tcgcccgccc cacccacaca tacctcggct tcaacgcggg
     3961 ccgggacttc gatcgggaga tcgtggtcaa ggtcaatgcc cccgaggtgg tccgggccga
     4021 actcgcgcga ccttcatgga agcgcgaact ggtcgccctc ggcaccaaca ccgaccccta
     4081 ccaatgggtc gaaagcaggt accggctgac ccgcggcgtg ctcgaagcgc tcgatgaggc
     4141 cgacaccccg gtctcggtcc tgaccaagtc accactggtg ctctccgacc tgccgatctt
     4201 cgaacggatg aaccaggccc ggccgaccgg agtgaacctc tcggtgccga ccgttgacga
     4261 aaaggcctgg cgggcgaccg aaccacacac cccgagcccg caggcccggc tcgaagccgt
     4321 ggccgagctg aggcggcggg gcatcccctc gggcatcctg atcgccccga tcatgcccgg
     4381 aatcaacgac cgtccggatc agatcgaacc gattctccag gcggccagcg atgccgacgc
     4441 cgacttcgtc accccgatcg cactcaacct ccgcaaggag acgcggaccg tgttcttcga
     4501 ctggctggag gccgagcgcc cggagctgct ccccgaatac gggcgccttt ttgacggccg
     4561 gtcccggctg ccgaagatcg agcaggagcg cgtcatcgcg gccgcccggg gccgttacc
//
LOCUS       DAOQMF010000039         4615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8866_length_4615_cov_3.252193, whole genome shotgun sequence.
ACCESSION   DAOQMF010000039 DAOQMF010000000
VERSION     DAOQMF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8866_length_4615_cov_3.252193"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1039
                     /locus_tag="PLV77_01155"
     CDS             200..1039
                     /locus_tag="PLV77_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC14486.1"
                     /translation="MEASMENKPTVAIITGASSGIGEGVAWRLARKPGTRLILVARRE
                     DRLRELAKRLSCEVTWIAADLTEPDAPGRILAHAQEHHPPVNLLVNNAGAAWRATFAE
                     GGYENVRRTMEINFDAVLRLTETFLPMLRESAPSSIVNIASTAGRVSRPGSAAYSASK
                     YALIGWSDGLHLEEAPHGVHVGLVLPGFIKTEGFPADELTGKFATRWMVSDVFKASDA
                     VYETAILGKAERYVPRPYELIAILKILAPGLVRKVLSGGAGKAMTTSTGADAAEQAEK
                     TRT"
     gene            complement(1097..1915)
                     /locus_tag="PLV77_01160"
     CDS             complement(1097..1915)
                     /locus_tag="PLV77_01160"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNC14487.1"
                     /translation="MNHLLRGHAPITDEAWKQIDEEARDGLVPALGGRRLVDFKGPLG
                     WGHSATDLGRVSDSTDGPADGVNLRTRQVLPVAELRAPFTISRSELEALDRGAVDTDF
                     DSLNAAARQMTIAENAAIFHGLGDAGMEGIAGASSHEPIPLGNNGFNDYPEHIAKAIE
                     VILSAGVEGPYGVALSPECYTGVVESAERGGYPLLSHIRKILQESGGGPLVWAPGLDG
                     VVVASMRGGDLLFESGQDLSIGYESHDAEKVNLYIEESFTFRVATPEAAVALPK"
     gene            complement(1912..2307)
                     /locus_tag="PLV77_01165"
     CDS             complement(1912..2307)
                     /locus_tag="PLV77_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14488.1"
                     /translation="MEFNQYHEPPEELSPELRTKARLFASLAEEAEAINWYEQRLSVE
                     PDAEARAIMKNAQHEEFKHFSMDLEFLLRKIPVWRELAEGVLFKDGDIVEHGEAAEAG
                     TDEEDEDATPEPAGSGGSLGIGSLRGAGL"
     gene            complement(2397..2960)
                     /gene="pyrE"
                     /locus_tag="PLV77_01170"
     CDS             complement(2397..2960)
                     /gene="pyrE"
                     /locus_tag="PLV77_01170"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNC14489.1"
                     /translation="MTDRDQTRQQLIGLLREHSLVLGEVTLSSGAKAGYYVDARRTVM
                     RPEGLKAAGTLIALHAKELGATAVGGPVMAAIPLACAAIAVPEGEGLGGFFVRKERKA
                     HGLQRWVEGAAEPGDRVLVVEDTVTTGGSTISAIERIRDEGFEIAGVLAVVDRLAGGG
                     EKIAAEAGAPYETLTTIDDIYPDRPDK"
     gene            complement(2986..3774)
                     /locus_tag="PLV77_01175"
     CDS             complement(2986..3774)
                     /locus_tag="PLV77_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNC14490.1"
                     /translation="MSQAMEANQPAAEPAAGTDPVVFPPSPPGVDPPEDPGKYPFATW
                     SPWVAIGGAVAALIFGVMLALPVDVVAGGENFDDYGWQVKALFQLCTVVGFLAVPFLF
                     AKELPGGVNWREAAGRLGFHRFRIGTAAKWIGLGFLAYIAFAIVYAVVFGAPDQDDIA
                     GELGPLWSQILLIVILAPLSEEVCFRGMLFGGFRTRFNLPVAAIGAGLVFGALHYSTG
                     ITAVPQLIVLGAIFAVVYEKSESIWPAIIFHLINNAYALAILNS"
     gene            3879..>4615
                     /locus_tag="PLV77_01180"
     CDS             3879..>4615
                     /locus_tag="PLV77_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNC14491.1"
                     /translation="MTRRQRRVRRHRGSPGKVFVAVAAVLGGLLLTAIAAAGGWVLSI
                     AADAPNPDNLKEINKGQNSVIYAGDGSKLGLINSTEIRTPIPLSKIPQSIQDATVAIE
                     DERFYQHDGVDVQGGIRALFKNLEAGEVNEGASTITMQLMRNIYISNPQRDIERKIKE
                     AKMALDYEKDHTKKQILEKYLNTAPYGTNGGRSAIGVNAAAKVYFSVKPENMSLPQAA
                     LLAGLPQAPSDYNPISNPAGAKARRNEV"
BASE COUNT          819 a         1599 c         1453 g          744 t
ORIGIN      
        1 cgaccagcgc cgccgacgtg gaccgccaca ccgaagtctt cggcgaggca gtagctgact
       61 tgctcgggtg atcaccccca acctgtctcg taaggtggcg gggggtgtgc ccggaaaacc
      121 tccggaatga ggagcgaagc gacggttttg ggggtacacc ccgcaccgcc cccaaccacg
      181 cggctatagg ctgcgcaacg tggaagcctc gatggagaac aaaccgaccg tcgcaatcat
      241 caccggagcc tcaagcggaa tcggcgaagg ggtcgcctgg cgcctggccc gcaagcccgg
      301 cacccgactg atcctggtcg cccggcgtga ggatcgcctg cgggaactgg ccaaacgcct
      361 gtcctgcgaa gtgacctgga tcgcggccga cctgaccgag ccggatgctc ccggccggat
      421 cctcgcccat gcccaggagc accacccgcc ggtcaacctg ctggtcaaca acgccggtgc
      481 cgcctggcgc gcgacctttg ccgaaggcgg ttacgagaac gtgcgccgga ccatggagat
      541 caacttcgat gccgtcctgc ggttgaccga gaccttcctg ccgatgctgc gggagagcgc
      601 ccccagctcg atcgtcaaca tcgcctcgac cgcgggccgc gtttcacgac cgggctcggc
      661 tgcctactcg gcctccaagt acgccctgat cggctggagc gacggactcc atttggaaga
      721 ggccccgcac ggcgtccacg tcggcctcgt cctgcccggc ttcatcaaga cggaaggctt
      781 cccggccgat gagctgaccg ggaagttcgc gacccgctgg atggtctccg acgtcttcaa
      841 ggcttcggac gcggtctacg agacggcgat actcggcaag gccgaacggt acgtgccgcg
      901 gccctacgag ctgatcgcga tcctcaagat cctcgccccg ggtctggtca gaaaggtcct
      961 gtccgggggt gccggcaagg cgatgaccac gagcaccggc gccgacgccg ccgaacaggc
     1021 cgagaagacc cgcacctgat ccgcgagaac ccgctttgtg ccgcataccg gcacaaaacg
     1081 ggcactcgac gaaccgctac ttcggcaggg cgaccgccgc ctccggcgtg gcgacccgga
     1141 acgtgaagct ctcctcgatg tagaggttga ccttttccgc gtcatggctc tcgtagccga
     1201 tcgagaggtc ctggcccgac tcgaagagca agtcgccgcc gcgcatgctg gcgaccacga
     1261 caccgtcgag tccgggcgcc cagaccagcg gaccgccgcc actctcctgc aggatcttgc
     1321 ggatgtgcga gagcagcggg taaccgccgc gctcggcgct ttcgaccacg ccggtgtagc
     1381 actcgggact gagcgcgacc ccgtaggggc cctcgacgcc ggccgagagg atcacctcga
     1441 tcgccttggc gatgtgctcc gggtagtcgt tgaagccgtt gttgccgagc gggatcggtt
     1501 cgtggcttga ggcaccggcg atcccttcca tgcccgcgtc tccgaggccg tggaagatcg
     1561 cagcattctc ggcgatcgtc atctggcggg ccgccgcatt cagggagtcg aagtcggtat
     1621 ccaccgcgcc gcggtccagc gcctcgagtt cagagcggct gatcgtgaac ggcgcccgaa
     1681 gttccgccac cggcaggacc tggcgggtcc tcaggttgac tccgtcggcc gggccgtcgg
     1741 tcgagtccga cacccggccc agatcggtcg ccgagtggcc ccagccaagg ggccccttga
     1801 agtccaccag ccggcgacca ccgagcgccg gcaccaggcc gtcgcgcgct tcctcgtcga
     1861 tctgcttcca ggcttcgtca gtaatcgggg cgtgaccccg cagaaggtga ttcaaagccc
     1921 cgctcctctc aggctgccaa ttccaagcga tccacccgat ccggccggtt ccggggtcgc
     1981 gtcttcgtct tcctcgtcgg ttccggcttc ggccgcctcg ccgtgctcga cgatgtcgcc
     2041 gtccttgaac aggactcctt ccgccagctc ccgccagacc ggaatcttcc gcagcaggaa
     2101 ctcgagatcc atcgagaagt gcttgaactc ctcgtgctgg gcgttcttca tgatcgccct
     2161 ggcttcggcg tcgggctcga ccgagagccg ctgctcgtac cagttgatcg cctcggcctc
     2221 ttcggccagg gaagcaaaca gtctcgcctt ggtcctgagt tcggggctca gttcctccgg
     2281 tggctcgtgg tactggttga attccacgcc tggctccttt cgacggatct cgttgccggt
     2341 gaactcatgc tctcactccg gggtccccgg gacaagcttc cggcaaaccg gacgccttac
     2401 ttgtcggggc ggtccgggta aatgtcgtcg atcgtggtca gggtctcgta gggcgcgccc
     2461 gcctcggcgg cgatcttctc gccgccgccg gccagccggt caaccacggc aagcacgccg
     2521 gcgatctcga aaccctcgtc acggatcctt tcgatcgccg agatcgtcga accgccggtg
     2581 gtcaccgtgt cctccaccac cagcacccgg tctccgggtt cggccgcgcc ttcgacccag
     2641 cgctggagtc cgtgcgcctt gcgttccttc cgcacgaaga aaccgcccag accctcgccc
     2701 tccggcaccg cgatcgccgc gcaggcgagc gggatcgccg ccatcaccgg accgccgacc
     2761 gcggttgcgc cgagttcctt cgcgtgcagg gcgatcaggg tgcccgccgc cttcagtcct
     2821 tccggtcgca tcaccgtgcg tcgggcgtcg acgtaataac ccgccttggc cccgctcgaa
     2881 agagtgacct cgcccagcac gagcgaatgc tcgcgaagca ggccgatcaa ctgctggcgg
     2941 gtctggtcac ggtcggtcaa ggtcgttcct ctcggtcatc caccctcagg aattgaggat
     3001 ggccagggcg taggcattgt tgatcagatg gaagatgatc gccggccaga tcgactctga
     3061 cttctcgtac acgacggcga agatcgctcc cagcacgatc agctggggca ccgcggtgat
     3121 tccggtcgag tagtgaaggg cgccgaagac cagtccggcc ccgatcgccg cgaccggcag
     3181 gttgaaccgg gtccggaaac cgccgaagag catcccccgg aaacagacct cctcggaaag
     3241 cggagccagg attacgatca ggaggatctg ggaccagagc ggtccgagtt cgccggcgat
     3301 gtcgtcctga tcgggagcgc cgaaaaccac ggcatagacg atcgcgaagg cgatgtaggc
     3361 gaggaatccg agcccgatcc acttggccgc cgtgccgatg cgaaagcggt ggaacccgag
     3421 ccggccggcc gcttcccgcc agttgacccc gcccggaagt tccttcgcga agaggaaggg
     3481 aaccgccagg aaaccgacca ccgtgcagag ctggaacagc gccttgacct gccagccgta
     3541 gtcatcgaaa ttctcgccgc cggccacgac gtcgaccggc agggccagca tcacaccgaa
     3601 gatcagggcc gcaacggctc cgccgatcgc gacccacggg ctccaggtcg cgaaggggta
     3661 cttcccggga tcttcgggcg ggtcgacccc gggtggggaa gggggaaaga cgaccgggtc
     3721 ggtcccggct gccggctccg ctgcgggctg gttggcttcc atggcttggc tcattctcac
     3781 aattcggggc cgcagtcgtc cgcaacccat cctgcaccgg cgtcgcctgg aaccgccctt
     3841 tagaagttcc tgaggtgctt ccagctaccc tttaggggat gaccaggcgc cagagaaggg
     3901 tgcgccgtca ccgtggttca cccggcaagg tttttgtcgc cgtggccgca gtgctcggcg
     3961 gattgctgct aaccgcgatt gcggctgccg gcggctgggt tctttcgatc gccgccgacg
     4021 ccccgaatcc ggacaatctc aaggagatca acaagggcca gaactcggtc atctacgccg
     4081 gtgacggcag caagctcggt ctgatcaact cgaccgaaat ccgcaccccg atcccgcttt
     4141 cgaagatccc gcagtcgatc caggacgcaa cggtcgcgat cgaggacgag cgcttctacc
     4201 agcacgacgg cgttgacgtc cagggcggca ttcgggccct gttcaagaac ctcgaggccg
     4261 gtgaggtgaa cgaaggcgcc tcgacgatca ccatgcagct gatgcggaac atctacatct
     4321 cgaacccgca acgggacatc gagcggaaga tcaaggaagc caagatggcc ctcgattacg
     4381 agaaggacca caccaagaag cagatcctcg agaagtacct gaacaccgcc ccctacggca
     4441 ccaacggcgg acgcagcgcg atcggcgtga acgcggccgc caaggtctac ttctcggtca
     4501 agccggagaa catgagcctg ccccaggccg ccctgctggc cggtctgccg caggccccct
     4561 cggactacaa cccgatcagc aatccggccg gggccaaggc ccgccgcaat gaagt
//
LOCUS       DAOQMF010000040         4592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8929_length_4592_cov_3.025127, whole genome shotgun sequence.
ACCESSION   DAOQMF010000040 DAOQMF010000000
VERSION     DAOQMF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8929_length_4592_cov_3.025127"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2520)
                     /locus_tag="PLV77_01185"
     CDS             complement(<1..2520)
                     /locus_tag="PLV77_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14492.1"
                     /translation="MSRRANLPHHPGLIAAASLVALVTACLILPVGARAAGETFVTTG
                     LPMPADGAPGADSGYSDFRPSGSPLSANGRYVAFQSDASFQLKGVRPDETNVFRKDRK
                     TGKVVLVSRRTGPAGAGSRGRTGSVTISGDGNLVGFTTTDQLVPADTDLVADFYIRNV
                     ATNVTTLESPGTDLEVGDGSISADGSYAVFDTTSVVAGVDVNNKPDVFRRRLSDGTTD
                     LVSRIPASATAGDNLSFAGGISGDGRWVSFTSGSSDLVTPFTDTNGAFTYDVFVRDMT
                     DGSTYLVSSVYNDASTTANGGSGEPVVAGSPASAAEVKLSFSSNATDLAPPGTDGATD
                     YSVYTRGLTSPASTLVSLNSSGENANSRAHTPSISNDGKRLVFTSDATNLGGPDTYYG
                     VYLRNLVTNQTSLVSARNEYAVFGSLSADGRVAAWAETGATKDADPFIEGIFARTLPK
                     GKITMASSPAGGKRMVLPGARLNDATIDGPQLSQNGRYEVLTVYTNRLPGYRKGFAQT
                     YRRDLKTGEWTLVSRATGKNGSPAETGSEGASISNDGNRVLFLTRTSLVPIDTDNELS
                     AYIRDLKKATTSLVSRADGASGANADQAVNSAVISGGGNRVAFSTYSTNLGAPGGQEQ
                     VYLRDLAGKKTLLVSRADGEAGAAGNGDSGNPELSDNGQLVVFDSLSTNLHPDDADAN
                     RSIFVRHPGSGSTVLASRRPGLTGANATDTQSSPDISGNGRFVAWRSGDPDTAPEAGP
                     WPIGTSQVVLRNLNSGENSLISRGPGSGPPGELGGASPSLNRNGSVIAFATNSANLIS
                     GLGGADRPAVMVRNLASGGQLSGPPAFGEVGSLASTGSLAPS"
     gene            complement(2530..3201)
                     /locus_tag="PLV77_01190"
     CDS             complement(2530..3201)
                     /locus_tag="PLV77_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14493.1"
                     /translation="MHRHLTPSLVLSVIAVVIALGGTSYAISQLPKNSVGARQLKKNA
                     VTAAKLKKDSVSSAKVKDGSLLKKDFKQGQIPKGQTGAAGATGASGPTGPSGPTGETG
                     ETGAAGSAVAYAYVQDIGDPNQSIPSDTAKNMQSSMFSKSQTNPTGVYCFDLESIWPI
                     KNFSVVAESNYNNLPESDKFATGQIQHNNDFGVGCPNTTDLLVITRDASTGNLVNWFF
                     YLTLN"
     gene            3275..3628
                     /locus_tag="PLV77_01195"
     CDS             3275..3628
                     /locus_tag="PLV77_01195"
                     /inference="COORDINATES: protein motif:HMM:NF025590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HNC14494.1"
                     /translation="MIQSPRRVLVLAAVLASAFALSACEKEVSIGDNTIDRDELELES
                     SRTLTKQYGEKPASIDCPDDLKAEVDATETCELVDQSGNHYDMKVTVTSVDDDGNAKF
                     NIKVGGIKQMGSVPE"
     gene            3647..3829
                     /locus_tag="PLV77_01200"
     CDS             3647..3829
                     /locus_tag="PLV77_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14495.1"
                     /translation="MANSSEDIQREFEEFEQAETSLDSGLESAPGRDRMPSWIKWIVI
                     ADLVIVIVVALIVFAA"
     gene            3923..4291
                     /locus_tag="PLV77_01205"
     CDS             3923..4291
                     /locus_tag="PLV77_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14496.1"
                     /translation="MIKRAFAVLAAALVLGVAGGAMASTASATIIPREGHYSGHDHNH
                     NHIGLNYTVHGQMNHFTIDYSFVIGGAHVSNAMWHPTCHNGYCTSGAWVNKTTIQGHY
                     WHGGNPHHKVPFIVHWIHSR"
BASE COUNT          766 a         1406 c         1562 g          858 t
ORIGIN      
        1 cgaaggggca agcgagccgg tcgaagccag gctcccgact tccccgaacg ccggcggccc
       61 gctgagctgg ccgccactgg cgagattgcg gaccatgacc gccggccggt cggcgccgcc
      121 caaaccgctg atcaggttgg ctgaattggt ggcgaaggcg atcaccgaac cgttccggtt
      181 gagactcggc gacgccccgc cgagttcacc gggcggcccg ctgccgggac cgcggctgat
      241 caggctgttc tctccgctgt tgaggtttct cagcacgacc tggctggtgc ctatgggcca
      301 cgggccggcc tccggggcgg tatccgggtc gccggatctc caggccacga accgcccgtt
      361 gccgctgatg tcggggctgg actgggtgtc ggtggcgttg gcaccggtga ggccggggcg
      421 gcgcgaggcg agcacggtcg agcccgaacc ggggtggcga acgaaaatgc tccggttggc
      481 gtcggcgtca tcgggatgga ggttggtcga aaggctgtcg aagacgacca gctgcccgtt
      541 gtcgctcagc tcgggattgc ccgagtcccc gttgccggcc gccccggcct cgccgtcggc
      601 gcgggaaacc agcagcgtct tcttgccggc aaggtccctc agatagacct gctcctggcc
      661 gcccggcgcc ccgaggttgg tcgagtaggt ggagaaagcg acccggttcc cgccaccgct
      721 gatcacggcc gagttgaccg cctggtccgc gttggcgccg cttgctccgt cggccctcga
      781 taccaggctc gtcgtcgcct tcttcagatc cctgatgtag gcgctgagct cgttgtcggt
      841 gtcgatcgga acgagagacg tgcgagtcag gaaaaggacc cggttgccgt cattgctgat
      901 tgaggcgcct tcggagccgg tttccgcggg tgaaccgttc ttcccggtgg cccggctgac
      961 cagggtccac tcaccggtct tcagatcccg ccggtaggtc tgggcgaagc ccttccggta
     1021 accgggcagc cggttcgtgt agacggtaag tacctcgtag cgcccgttct ggctgagttg
     1081 cggaccgtca atggttgcgt cgttgagcct cgcgccgggc aggaccattc gcttgccgcc
     1141 agccgggctg gacgccatcg tgatcttccc cttcggcagg gtgcgagcga agattccttc
     1201 gatgaacggg tccgcatcct tggtcgcgcc agtctcggcc catgcggcta cacggccgtc
     1261 ggcgctgagg ctcccgaaga ccgcgtactc gttgcgagcg gaaaccagac tggtctggtt
     1321 ggtgaccagg tttcgcagat agacgccgta gtaggtgtcg gggccgccga gattggtcgc
     1381 gtcggaggtg aagacaaggc gtttgccatc gttgctgatc gaaggggtgt gggcccggct
     1441 gttcgcgttc tcgccgctgg aattgaggct gaccagggtt gacgcggggg aggtgaggcc
     1501 gcgggtgtag acggagtagt ccgtggcgcc gtcggtgccg ggcggcgcga ggtcggtcgc
     1561 gttcgaggag aaggagagct tgacctcggc ggcgctggcc ggactgcccg cgaccaccgg
     1621 ctctcccgag ccgccgttgg cggtggtgct ggcgtcgttg tagacggaac tcaccaggta
     1681 ggtgctgccg tcggtcatgt cgcggacgaa cacgtcgtag gtgaaggccc cgtttgtatc
     1741 ggtgaatggg gtgaccagat cggaagagcc gctcgtgaag gagacccagc ggccgtcccc
     1801 cgagatgccg ccggcgaagg aaaggttgtc accggccgtg gcggaagccg gaatcctcga
     1861 aacgaggtcg gtggtgccgt ccgaaagcct gcggcgaaag acgtccggct tgttgttcac
     1921 atcgactccg gccacaacgg aggtggtgtc gaacaccgcg tagctgccgt cggcagagat
     1981 cgagccgtcg ccgacttcga ggtcggtgcc cggcgactcg agtgtggtca cgttcgtggc
     2041 tacgttccgg atgtagaagt cggcgacgag gtcggtgtcc gcgggaacga gctggtcggt
     2101 cgtggtgaag ccgaccagat ttccatcccc gctaatcgtc acgctgccgg tgcgtcccct
     2161 gctgcccgcc ccggccggac cggttcgacg gctgaccagc acgaccttgc cggtcttccg
     2221 gtccttgcga aacacgttcg tttcgtccgg ccggactccc ttgagctgga agcttgcgtc
     2281 ggactggaag gccacgtaac ggccgttcgc actgagtggg ctgccggaag ggcggaagtc
     2341 gctgtagccg gaatcggccc cgggtgcacc gtcggcaggc atcggaagcc cggtcgtgac
     2401 gaaggtctcg ccggcggccc gggcgccgac cggaagaatc aggcaggcag tgaccagcgc
     2461 gacgagtgac gccgcggcga tcagtcccgg atgatgcgga aggtttgctc tccttgacat
     2521 gaccggcccc tagttgaggg tgaggtagaa gaaccagttg acgaggttgc ccgtgctcgc
     2581 atcccgggtg atgaccaaca ggtcggtcgt gttcgggcag ccgaccccga agtcgttgtt
     2641 gtgctggatc tggccggtcg cgaacttgtc gctctcgggc aggttgttgt agttcgattc
     2701 ggccacgacc gagaagttct tgatcggcca gatcgactcg aggtcgaagc aatagacccc
     2761 ggtcgggttt gtctgcgact tggaaaacat cgagctctgc atgttctttg cagtgtcgga
     2821 ggggatggac tggttgggat caccgatgtc ctggacgtag gcgtaggcca cggccgaacc
     2881 ggcggctccg gtctccccgg tctcgccggt cggcccgctg ggtccggtcg gcccgctggc
     2941 cccggtcgca ccggcggcac cggtctgtcc ctttggaatc tggccctgct tgaagtcctt
     3001 cttcagcagg gagccgtcct tgaccttggc cgaactgacc gagtccttct tgagcttggc
     3061 ggcggtcacc gcgttcttct tcagttgtcg ggccccgacc gaattcttcg gtagctggct
     3121 gatcgcgtag ctggttcctc ccagggcgat caccacggcg attactgaaa ggacgagtga
     3181 cggtgtcagg tgcctgtgca tgaccccaat ctacggccgc cggcggcgga gggcaaggga
     3241 ttccagccgc cccgtcggcc ggtagggtgg gcgcatgatc cagtctcccc gccgtgtcct
     3301 cgtcctggcc gcagtgctcg cgtcggcctt tgccctcagc gcctgtgaga aggaagtttc
     3361 gatcggtgac aacacgatcg accgggatga gctcgagctg gagtcttcac gcaccctgac
     3421 caagcagtac ggggagaagc ccgcttcgat cgattgtccc gacgacctca aggccgaggt
     3481 tgacgcgacc gagacctgcg aactggtcga tcagagtgga aaccactacg acatgaaggt
     3541 gaccgtcacc tcggtcgacg acgacggcaa cgccaaattc aacatcaagg tcggcggcat
     3601 caagcaaatg ggttcggttc ccgaataggg ccgggccgcg aacgccatgg ctaattccag
     3661 cgaagacatc cagcgggagt tcgaggagtt cgaacaggcc gagacttcgc ttgattccgg
     3721 gctcgagtcg gctccgggcc gggaccggat gccttcgtgg atcaagtgga tcgtcatcgc
     3781 cgacctggtc atcgtgatcg tggtcgcgct gatcgttttc gccgcctgaa acgaatttac
     3841 cgaacggcgt tcggaagttc gtttatagtc ggattaccga ccaatgcggg gttcgtccgc
     3901 tccgctacag aaagagggat ccttgatcaa acgcgcattc gctgtcctcg ccgcggcact
     3961 cgtgctcggc gttgccggag gggcaatggc atcgaccgcc agcgccacaa tcattcccag
     4021 ggaggggcac tactcgggcc acgaccacaa ccacaatcac atcggcctca actacacggt
     4081 tcacggtcag atgaaccact tcacgatcga ttacagcttc gtgatcggtg gggcgcatgt
     4141 gagcaacgcg atgtggcatc cgacctgcca caacgggtac tgcaccagcg gtgcctgggt
     4201 gaacaagacc accatccagg gtcactactg gcatggcggc aacccgcatc acaaggttcc
     4261 gttcatcgtc cactggatcc actcccgcta acacggaagt gattcgggga gaggccggcg
     4321 ggagaccgcc ggcctttttc ttgtccagtc cggacggggc gcctatattg gtctccgacg
     4381 gagcgaacta ccgcttcgca tcaccaggga gggcaacttg atcagacgag caatctcaat
     4441 cctgtcggtg gcgctgcttt taggcgcgtc cggtgcggcg gcatcgagca cggccgacgc
     4501 ggtggtaccg cgcgagggcc attattcggg tcacgatcac gaatggaatc cgatcggcct
     4561 gaactacagc cgcaccttcc aggtcaaggt ga
//
LOCUS       DAOQMF010000041         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8984_length_4576_cov_3.708472, whole genome shotgun sequence.
ACCESSION   DAOQMF010000041 DAOQMF010000000
VERSION     DAOQMF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8984_length_4576_cov_3.708472"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLV77_01210"
     CDS             complement(<1..691)
                     /locus_tag="PLV77_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNC14497.1"
                     /translation="MTDPVITDYHLHLRGDELSARAEDHFTTENIARYLEAAGQVGVT
                     DLGCAEHMYRFTEALTVWRHPFWEEWAIDDIDQYVEAVRDSPIKLGIEGDFIVGAEEK
                     LESMLGSRPFDYVVGSVHFLGDYSLDTEDYTIWDGSQDPDEIWRRYFETLASAARSGL
                     FDILAHPDLVKVWGRAGRLPSVDPRTMYEPAIEAIAETGIAVEVSTAGLRKGVEEIYP
                     APAFMEMCVEAG"
     gene            complement(688..1359)
                     /gene="ispD"
                     /locus_tag="PLV77_01215"
     CDS             complement(688..1359)
                     /gene="ispD"
                     /locus_tag="PLV77_01215"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNC14498.1"
                     /translation="MGVPALIVAAGSGQRLGAGGPKALVELSGRPLYAWSVDAFRRAT
                     RIDRIFVAVPPGQAGQFEEPGIITVEGGETRSHSVANGLAAITADGDPGMVVVHDAAR
                     PLVSPALIDAAVIGLDSDPELHALIAAAPMTDTVKRLGEDGIVVETLDRSELVSVQTP
                     QVFRIPNLQAAIAAGDLSAATDDASLVEAVGGSVGVIESPSGNIKVTVPADLELAALL
                     LERSR"
     gene            complement(1385..1951)
                     /locus_tag="PLV77_01220"
     CDS             complement(1385..1951)
                     /locus_tag="PLV77_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNC14499.1"
                     /translation="MNGEEVTDEDLDLTPYVCDFEEGDKVVYPHHGAGVVLKKADAEL
                     MGETREYLTIKILHNDLTVKVPTAKAGEAGLRRVIDEDEIKKVISVLSDEVSDMPKNW
                     NRRFKYNKEKIKTGDVFELAEVVRNLALREMEKGLSTGEKQMYTRAKKILASEFMYAL
                     DKDEEGAESYLDGLLEERYEASVAAAAA"
     gene            complement(2011..3123)
                     /gene="disA"
                     /locus_tag="PLV77_01225"
     CDS             complement(2011..3123)
                     /gene="disA"
                     /locus_tag="PLV77_01225"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HNC14500.1"
                     /translation="MSPAVESDLAHLSERQDPRLLRAIDAVAPGTELREGIDNILHAR
                     TGGLIVVGETEDFAFMLSGGIKLDIDYTPAMLYQVAKMDGAILVNADGSKITWANVQM
                     MPDPTIHSSETGTRHRTAERISKQVDALVIAISERREVVSLYLEGVKYILQDIPAVLA
                     KSNQALATLTKYRTRLDELSSRLTRAEFVGGAVLYDALMVLQRSELVSRMATEVERYV
                     IELGTEGRLIEMQLEETMVGVASERLALMRDYAVEDTEENVQFMLEELAALPHQDVLD
                     FGRLAELLGYDRKVNTLDLATEPRGYRMLGHVPRLPRLVVQKIVMNFGSVEAALAASD
                     EELAAVDGVGPARAKEIREGVRRLQESVMADRFLNT"
     gene            complement(3247..>4576)
                     /gene="radA"
                     /locus_tag="PLV77_01230"
     CDS             complement(3247..>4576)
                     /gene="radA"
                     /locus_tag="PLV77_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNC14501.1"
                     /translation="EWNTLVEQAAPRPAAKAPGGKSGNGRALAAGAAGQVLPLKDVRA
                     SRVERFPTGIGEFDRVLGGGLVPGSLVLLGGSPGIGKSTLTGMAMANLQAGGIETLYV
                     SGEESAAQVRMRAERLGPAALEVKVLAESSLEVVLGTIESERPAVCVIDSIQTMHSSE
                     LSGAPGSVGQVREAANALAEQAKRLEVAVILVGHVTKEGSLAGPRVLEHLVDCVLRFE
                     GERERTWRTLRAVKNRFGATSEVGVFEMRSGGLTEVLDPSARFVEEATGAQGSVVLAS
                     MEGTRPVLVEVQALVSPSELEIPRRVVNGIDRNRLSMILAVLARHARLGLGTADVFVN
                     VVGGVRIDEPGADLAVALAVASAQRGETLSGPDGRPLACFGELGLTGDLRYVAHADRR
                     VAEAAKFGLEPVIGPRGGERLSGLAPCATVKQALKAAFSGSSADSRAAAA"
BASE COUNT          736 a         1572 c         1435 g          833 t
ORIGIN      
        1 cccccgcctc gacgcacatc tccatgaacg ccggggcagg gtagatctcc tccaccccct
       61 tgcgcaggcc cgcggtcgat acctcgacgg cgattccggt ctccgcgatc gcttcgatcg
      121 ccggctcgta catggtcctc ggatcgaccg agggaaggcg tccggcccgg ccccagacct
      181 taaccaggtc gggatgggcc aggatgtcga agagcccgga gcgggccgcg ctggcgaggg
      241 tctcgaagta gcggcgccag atttcatcgg gatcctggct gccgtcccag atcgtgtagt
      301 cctcggtgtc aagcgagtag tccccgagga agtgaaccga tccgaccacg tagtcgaagg
      361 ggcgcgagcc gagcatcgac tcgagcttct cctccgcccc gacgatgaaa tcaccctcga
      421 tcccgagttt gatcgggctg tccctgaccg cctcgacgta ctggtcgatg tcatcgatcg
      481 cccattcctc ccagaaggga tgccgccaga cggtcaaggc ctcggtgaag cggtacatgt
      541 gctcggcaca gccgaggtcg gtgactccga cctgccccgc cgcttcgagg tagcgcgcaa
      601 tgttttcggt cgtgaagtga tcttccgccc gggccgagag ttcgtcaccg cgcaggtgga
      661 ggtgatagtc ggttatcact gggtcggtca tcggcttctt tcgaggagga gagcggcgag
      721 ttcaaggtcc gcggggaccg tgaccttgat gttgcctgaa ggcgattcga tcacgcccac
      781 cgaccccccg accgcctcga ccagcgaggc gtcgtcggtc gccgcggaaa ggtccccggc
      841 cgcgattgcc gcctggaggt tcgggatccg gaagacctgt ggcgtctgca ccgagaccag
      901 ttcggaacga tcgagggtct cgaccacgat gccgtcctcg ccgaggcgtt tgaccgtgtc
      961 ggtcatcgga gccgcggcga tcagcgcgtg gagctcgggg tcggagtcca ggccgatcac
     1021 ggcggcgtcg atcagggccg gactgaccag cgggcgggcg gcatcgtgaa cgaccaccat
     1081 gccgggatca ccgtccgcgg tgatcgccgc cagaccgttg gcaaccgagt gggaacgggt
     1141 ctcgcccccc tccaccgtga tgattcccgg ttcctcgaac tggcccgcct gaccgggcgg
     1201 gaccgcaacg aagatccggt cgatgcgggt cgcccggcga aatgcgtcga ccgaccacgc
     1261 atagagcgga cggccggaca gctcgaccag ggctttgggc ccgccggctc cgagcctttg
     1321 cccggagccg gcggccacga tcaatgccgg cacccccatc tcggggaccg ttcaggccgg
     1381 cggcctaggc cgcggcggct gccaccgagg cttcgtagcg ctcttcgagc aggccgtcga
     1441 ggtagctttc ggctccctct tcgtccttgt cgagtgcgta catgaactcg gaggcgagga
     1501 tcttcttggc ccgggtgtac atctgcttct caccggtcga gaggcccttc tccatctcgc
     1561 gaagcgcgag gttgcgtacc acctcggcca gctcgaagac gtcacccgtc ttgatctttt
     1621 ccttgttgta cttgaaccgg cggttccagt tcttcggcat gtcggaaacc tcgtccgaca
     1681 gcaccgaaat gaccttcttg atttcgtcct cgtcgatcac ccggcgcagc ccggcttcgc
     1741 cggccttcgc ggtcgggacc ttgacggtca ggtcgttgtg caggatcttg atcgtcaggt
     1801 actcgcgggt ctcgcccatg agttcggcat cggccttctt cagaacaacg cctgctccgt
     1861 ggtgcggata gacgaccttg tcgccttcct cgaaatcgca cacgtacgga gtcagatcga
     1921 gatcttcatc ggtcacttct tcgccgttca ctgcctctga atcggaaatc agatgctcct
     1981 ttgtctctta gctgacgact gaatgcgtcg tcatgtgttc aaaaaccggt ctgccatcac
     2041 ggactcctga agcctccgca ctccttcgcg gatctccttg gcccgggccg ggccaacgcc
     2101 gtcgacggcg gcaagttcct cgtcgctggc cgcgagcgcg gcttcgacgc tgccaaagtt
     2161 catgacgatc ttctggacca cgagccgcgg caggcgcgga acgtggccga gcatcctgta
     2221 gccgcgcggt tcggtggcca ggtcaagcgt gttgaccttg cggtcatagc cgagcagttc
     2281 ggccaggcgg ccgaagtcga gcacgtcctg atgcggcagg gcggccagtt cctcgagcat
     2341 gaactggacg ttctcctcgg tgtcctcaac cgcgtaatcg cgcatcagtg cgagccgctc
     2401 cgaagcaacc ccgaccatgg tctcctcgag ctgcatttcg atcagcctgc cttcggtgcc
     2461 gagctcgatc acgtatcgct cgacctcggt cgccatccgg gaaaccagct ccgagcgctg
     2521 cagcaccatc agtgcgtcgt agaggacggc gccgccgacg aattcggccc gggtcaggcg
     2581 gctcgagagc tcgtcgagcc gggtccggta cttggtcagc gtggcgaggg cctgattgga
     2641 cttggccagc accgcgggaa tgtcctgaag gatgtacttg accccttcga gatagagcga
     2701 gaccacttcc cgccgttccg agatcgcgat gaccagggca tcgacctgct tcgagatccg
     2761 ttcggcggtg cgatggcggg tgccggtctc cgaggagtgg atggtcgggt cgggcatcat
     2821 ctggacgttg gcccaggtga tcttgctgcc gtcggcgttg accaggatcg ccccgtccat
     2881 cttcgccacc tggtagagca tcgccggcgt gtagtcgatg tccagcttga tgccaccgga
     2941 aagcatgaag gcgaagtcct cggtttcccc gaccacgatc agaccgccgg tacgggcgtg
     3001 gaggatgttg tcgatccctt ccctcagctc ggtgccgggg gcgacggcgt caatcgcgcg
     3061 cagcagtctg ggatcctgac gttcagacag atgtgccaga tcactctcta ccgctggtga
     3121 cattcagaga attatgccag attcggcttt ctaatgcggt ctggcgtggt tccgggcgct
     3181 cttttgcggg ggttgtgacc ggccctcaat ccgctccgga aagccgaatt tagcctcgcc
     3241 tgaagatcag gctgcggcgg cccgggaatc ggctgaactt ccggaaaatg cggctttcag
     3301 ggcctgcttc acggtggcgc agggagcgag ccccgatagc cgctctccac cccttgggcc
     3361 gatcaccggt tcgaggccga atttcgctgc ctcggcgact ctgcggtcgg catgggcgac
     3421 gtacctcagg tccccggtca ggcccagttc accgaaacag gcgagcggac ggccatcagg
     3481 cccggaaagg gtctcgccgc gctgggccga agcgaccgcg agcgccacgg cgagatcggc
     3541 cccgggctca tcgatcctca ccccgccgac cacgttcaca aagacgtcgg ccgtaccgag
     3601 acccagcctc gcgtgccggg ccagcaccgc aaggatcatg ctgaggcggt tgcggtcaat
     3661 gccgttgacg acccttcgcg gaatctcgag ttcactcggg gagaccaggg cctgcacctc
     3721 gaccagcacc ggtctggtcc cttccatcga ggccagaacc accgacccct gggccccggt
     3781 cgcttcctcg acgaagcggg ccgacggatc cagcacttcg gtcaggcctc ccgagcgcat
     3841 ctcgaacacc ccgacctccg aggtcgcccc gaacctgttc ttaaccgccc gcagggtgcg
     3901 ccatgtacgt tcccgttcgc cctcgaagcg aagcacgcag tcgaccaggt gttccagcac
     3961 cctcggcccc gccaggctgc cttccttggt cacgtggccg accaggatca ccgccacttc
     4021 gagccgcttg gcctgctcgg cgagggcgtt ggccgcctcc ctgacctgcc cgaccgaacc
     4081 gggagcgccg ctcaattccg aactgtgcat ggtctggatc gaatcgatca cgcagaccgc
     4141 cggccgctcc gactcgatcg ttcccagcac cacctccagc gagctctcgg ccagcacctt
     4201 gacttccagg gccgccggcc cgagccgctc ggcccgcatc cgcacctgcg ccgccgattc
     4261 ctcaccggag acgtaaagcg tttcgattcc cccggcctgg aggttcgcca tcgccatccc
     4321 ggtcagggtg ctcttgccga ttcccggcga gccgccgagc agcaccagtg accccggaac
     4381 cagcccgccg cccaggaccc ggtcgaactc gccgatcccg gtcgggaacc gttcgacccg
     4441 ggaagcccgt acatccttca gcggcagtac ctggccggcg gcgccggccg cgagggcccg
     4501 gccgttgccg gacttgccgc cgggggcctt cgctgccggc ctgggcgcgg cctgctcgac
     4561 cagcgtgttc cattcc
//
LOCUS       DAOQMF010000042         4573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_8997_length_4573_cov_3.957282, whole genome shotgun sequence.
ACCESSION   DAOQMF010000042 DAOQMF010000000
VERSION     DAOQMF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8997_length_4573_cov_3.957282"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="PLV77_01235"
     CDS             complement(<1..1634)
                     /locus_tag="PLV77_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC14502.1"
                     /translation="MANPEITVCGRLTVAWDGEQLEGELPGRQGRLIFAYLVLNRSRP
                     VRRDELVEALWADEGLPSGGEALLAPPLSRLRKALGKDRLQGRADLSLQLGEEARIDF
                     EVAQQKLASAQNVAGNPDATADQLEAGWDDSREAAGILEGGLLPGLEARWIEEHRLYF
                     EELRLKSLETVARIGARLGTAQQAQAERAARNAVEASPFRESARAALIELLEAQGNIA
                     EALRAYEEMRVLLRDELGTFPAPELTAIHERLLNAHETPAAATGAPAPARPAPAPKPA
                     VTPKVIGQEIDPRVEGGFLIGREHILDQLRAELDLAASGELRIALLAGEGGVGKTRLA
                     AELASSRDDITVLYGRSEPDEVRPFRIWSGLLQSAVRQLGDIDPAEIVGGDGPTLSRL
                     LPELTRRMGLPAPGPTSDLESERQALFGAVMRMIGRFSARRPMLIILDDLHWADRSTL
                     RLLASMAGENPLRSILAVGIYRDTELPPESHLPETLSQLQRRLPTTRLQVQALDEAEV
                     RDLISGRLDEKLAPLIRDQTGGNPFFIEQMIRNLEES"
     gene            complement(1676..>4573)
                     /locus_tag="PLV77_01240"
     CDS             complement(1676..>4573)
                     /locus_tag="PLV77_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC14503.1"
                     /translation="EALRAYDEFRVLLRDELGTFPAPELNAVHERLLNAHEGVAAEKD
                     TGSQTASPEAAAAVPAERQATPEQIGRLIDPRIGEIGLVGREEILAQLNHELDLAVAG
                     DLRIALLAGDGGMGKTRIAAELAAGRDDVTVLYGRSEPDEIRPFRIWSGLLRSAMRQA
                     GDIPPAEIVGGDGPTLARILPELVRSMELPAPGPAGDLESERRALFGAVMRMIGRLTA
                     RQPMLIVLDDLHWADRSTLMLLASLAGDNPPGGVLALGIYRDTELPEDSLLPETLTNL
                     QRRLPTLKLEVEALGSEDVGQLIGGRLDAALAGSLHDQTGGNPFLIEQLVRHLEETGI
                     GDPGNAPAEVRQIISQRVNHLPDGGPDLLRRAALIGRDFDLSILLETTIWDEDSVIDL
                     LDAAVAAGLLDESPTVPGRYSFVHALLRSTLEEELGLTRRAMIHRDIGEAIERQTASR
                     PEKRVGEKAWHFTQAGPAEADRAVHWATQAAEQAEARLAYDEAVDFFDGAIAAARADE
                     PVDQGRLARLLLSQAKAKWKNGALQAAGDTFLEAAKAARESGLPELAAQAAIGSRWGG
                     WDAFDADLAEQLSLMRQALGSLPPVDSPLRAELMAMLGHTLYYGSGQADEARQLAAEA
                     IEMISRIDAPEAEFGVIQGTAFLRWQPLNRDQRLPASERMLEIAERLDDHELIGQALV
                     SRQLSLLNLGRGAEAEADRSRHYAMSETLPQIRVISKAMRACSWFMTGDWQDGEDLFS
                     ELEAEGMPRGAALLLEDALRFMVRAQQGALAEHLGRLELFEAGAKGWEMWPTWRLGLL
                     LAKAQAGQLDEVRELIAEKGYESLDETRDINATLLPFCAVGNMLAGELEDRDGAAKLV
                     DLLTPFSGEWIMIARIGSTLGPVDMHLGELHLLCGEHREAATALERSLVACEAMDAVP
                     YLARTKLALASAFGEMGDPDGQERRMELRNEGEQVAKRLDMQAILRRHIRS"
BASE COUNT          639 a         1584 c         1576 g          774 t
ORIGIN      
        1 gattcctcga ggttccggat catctgctcg atgaagaagg ggttgccgcc ggtctggtcg
       61 cggatcagcg gcgccagctt ctcgtcgagt cgacccgaaa tcaggtccct gacttcggct
      121 tcgtcgaggg cctggacctg gaggcgcgtg gtcggcaacc ggcgctgcag ctggctcagg
      181 gtctccggca gatggctctc gggcggcagt tccgtatcgc ggtagatgcc gaccgcgaga
      241 atgctccgca gcggattctc gccggccatg ctggccagca ggcgcagcgt cgaacggtcg
      301 gcccagtgca ggtcgtcgag gatgatcagc atcggtcgcc tcgccgagaa ccggccgatc
      361 attctcatca ccgctccgaa cagggcctgt cgctcggatt cgaggtccga ggtcggtccc
      421 ggagccggca ggcccatgcg gcgggtcagc tccggcaaaa gccgcgaaag ggtcgggccg
      481 tcgcccccga cgatttcggc cgggtctatg tcaccgagct gacgcacagc cgactgcagc
      541 agccccgacc agatccggaa gggacgcacc tcatccggtt ccgagcgacc gtagaggacg
      601 gttatgtcat cccggctgga ggccagctcc gccgccagcc tggtctttcc gactccgccc
      661 tcgccggcca gcagggcaat cctcagttcc ccgctcgccg cgaggtccag ttcggccctg
      721 agctggtcga ggatgtgctc cctcccgatc aggaaaccgc cctcgacgcg ggggtcgatc
      781 tcctgcccga tcaccttcgg tgtcacggca ggtttgggcg ccggggcggg ccgggccggg
      841 gccggggccc cggtcgcggc ggccggagtc tcgtgggcgt tcaggaggcg ctcgtggatc
      901 gcggtcagct ccggagccgg gaaggtgccg agctcgtcgc gaagcagcac ccgcatttcc
      961 tcgtaggccc gcagcgcctc ggcgatattc ccctgggctt cgagcagctc gatcagcgcg
     1021 gcccgggccg attcgcgaaa cggggaggcc tctaccgcgt tccgggccgc ccgttctgcc
     1081 tgggcctgct gggcggttcc gaggcgggcc ccgatccggg ccacggtttc gagcgacttg
     1141 aggcgaagct cctcgaagta gagccggtgc tcctcgatcc agcgggcttc caggccgggc
     1201 agcaggccgc cctcgagaat gccggcggct tcacgggagt cgtcccaccc ggcttcgagc
     1261 tgatccgctg tcgcatccgg gttcccggcc acgttctggg ccgaggcgag cttctgttgg
     1321 gcaacctcga agtcgatccg tgcttcctcc ccgagctgaa gcgaaaggtc ggcccggccc
     1381 tgcagccggt ccttgcccag ggccttccgc agccgggaga gcggaggcgc cagcaacgcc
     1441 tcgccgccgc tgggcaggcc ctcgtcggcc catagcgctt cgaccagttc atccctgcgc
     1501 acgggtcggg aacggttcag caccaggtag gcgaaaatca gtcgcccctg acggccggga
     1561 agctctcctt ccagttgctc accatcccat gccacggtga gccggccgca aaccgttatt
     1621 tccggatttg ccactgcggc aacttatcca accggcccgg tcggcgcgaa tcggttcagg
     1681 accggatgtg acgcctgagg atcgcctgca tgtcgagtct cttcgcgacc tgctcgccct
     1741 cgtttcgaag ctccatcctt cgttcctgcc cgtccggatc gcccatttcc ccgaaggctg
     1801 aggcgagggc cagtttggtt cgcgcgaggt aggggacggc atccatcgcc tcgcaggcca
     1861 ccagcgaccg ttccagcgcg gtagctgcct cgcgatgctc tccgcaaagg aggtggagct
     1921 ccccgaggtg catgtcgacc ggcccgagag tcgacccgat ccgggcgatc atgatccatt
     1981 cgccgctgaa gggggtgagc agatcgacca gcttcgccgc cccgtcgcgg tcctccagct
     2041 cgccggccag catgttgccg accgcgcaga aggggagcag ggtcgcgttg atgtccctcg
     2101 tctcatccag gctttcatag cccttctcgg cgatcagttc gcgaacttca tcgagctggc
     2161 cggcctgggc cttggcgagc aggagtccga gccgccaggt cggccacatc tcccagcctt
     2221 tcgcgcccgc ctcgaagagc tcgagccggc cgaggtgctc ggccagggcg ccctgctgcg
     2281 cccggaccat gaagcggagc gcgtcttcga ggagcagggc ggccccgcgg ggcatccctt
     2341 ccgcctcgag ttcgctgaag aggtcctcgc cgtcctgcca gtcgccggtc atgaaccagg
     2401 agcaggcccg cattgccttt gaaatcaccc ggatctgcgg cagtgtttcg ctcatcgcgt
     2461 agtggcgact gcgatcggct tcggcttcgg caccgcggcc gaggttgagc agggaaagct
     2521 gccgggagac cagggcctgg ccgatcaatt catgatcgtc gagacgctcg gcaatttcca
     2581 gcatccgctc cgaggccggg agtctctgat cccggttgag tggctgccag cgcagaaagg
     2641 cggttccctg aatcacgccg aattcggctt ccggagcgtc gatgcgggag atcatctcga
     2701 ttgcctcagc ggccagctgc cgggcttcgt cggcctggcc gctgccgtag tagagggtgt
     2761 gacccagcat cgccatcaat tcggcccgga gcgggctgtc caccgggggc aggcttccca
     2821 gggcctgtct catcagcgag agctgctcgg ccagatcggc gtcgaaggca tcccagccgc
     2881 cccagcggct gccgatcgcc gcctgggcag ccagctcggg cagacctgat tcccgggccg
     2941 ccttcgccgc ctcgaggaag gtgtcgccgg ctgcctgcag ggctccgttc ttccatttcg
     3001 ccttggcctg gctgagcagc agccgggcca gccgtccctg gtcaaccggt tcgtcggccc
     3061 gggccgcggc gatcgccccg tcgaagaagt cgaccgcctc gtcgtaggcg agccgtgctt
     3121 ccgcctgttc agccgcctgg gtcgcccagt ggacggcccg gtcggcttcg gcaggccccg
     3181 cctgggtgaa atgccaggcc ttctccccga cccgcttctc cgggcggctc gcggtctggc
     3241 gttcgatcgc ttcgccgatg tcgcggtgga tcatcgcccg gcgggtcaac cccagttcct
     3301 cctccagggt gctgcggagc agggcgtgta cgaacgagta gcggccgggg acggtcgggg
     3361 attcgtcgag cagcccggcg gcgacggcgg cgtcgagcag gtcgatcacc gagtcctcgt
     3421 cccagatggt cgtctccagg aggatcgaaa ggtcgaagtc gcggccgatc agggcggccc
     3481 ggcggagcag gtcggggccg ccgtcgggca ggtggttgac ccgctgggag atgatctgcc
     3541 ggacctcggc cggggcgttt ccggggtcgc cgatgccggt ctcctcgagg tgccggacca
     3601 gctgttcgat caggaagggg ttgccgccgg tctggtcgtg aagcgaaccg gccagggccg
     3661 cgtccagtcg gccgccgatc agctggccga cgtcttcgct tccgagcgct tcaacttcca
     3721 gcttcagggt cggcagccgg cgctggaggt tggtcagggt ctccggcagc aggctgtcct
     3781 cgggcagttc cgtgtcccgg tagatgccca gggcgagcac cccgccgggc gggttgtcgc
     3841 cggccaggct ggccagcagc atcagggtcg agcgatccgc ccagtgaagg tcgtcgagca
     3901 cgatcagcat cggttgccgg gcggtcagcc ggccgatcat tcgcatcacc gcgccgaaca
     3961 gggcccggcg ctcggactcg aggtcaccgg ccggtcccgg ggccggcagt tccatgctgc
     4021 ggaccagctc cggcaggatc cgggccaggg tcgggccgtc gccgccgacg atttcggccg
     4081 gggggatgtc cccggcctgc cgcatcgccg agcggagcag gcccgaccag attcggaagg
     4141 ggcggatctc atccggttcc gagcggccgt agaggacggt cacgtcatcc ctcccggcgg
     4201 ccagctcggc ggcgattctg gtcttgccca tgccgccgtc gccggccagc agggcgatcc
     4261 gcaggtcacc cgcgaccgcc aggtccagtt catggttcag ctgggccagg atctcctcgc
     4321 ggccgaccag cccgatttcg ccgatccggg ggtcgatcag ccggccgatc tgctccgggg
     4381 ttgcctgccg ttcagccggg accgcggccg cagcctccgg gctggcagtc tgcgagcccg
     4441 tgtccttttc cgcggcgacg ccctcatgtg cgtttagcag ccgctcgtgg acggcattca
     4501 gttccggggc gggaaaggtt cccagttcgt cgcggagcag gacccggaac tcgtcgtagg
     4561 cccggagcgc ttc
//
LOCUS       DAOQMF010000043         4411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_9551_length_4411_cov_4.452250, whole genome shotgun sequence.
ACCESSION   DAOQMF010000043 DAOQMF010000000
VERSION     DAOQMF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9551_length_4411_cov_4.452250"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLV77_01245"
     CDS             complement(<1..376)
                     /locus_tag="PLV77_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14504.1"
                     /translation="MAELEQVNDCCTPEAQASCCEAEARDECCGEEQGCGCQEGSERI
                     ESELREIVRERYAAAAVDAGSRDASCCANDTVITEEQRAVFGSNLYEEGDRSELPEAA
                     QLASLGCGNPTAVADLEEGQTVL"
     gene            complement(364..717)
                     /locus_tag="PLV77_01250"
     CDS             complement(364..717)
                     /locus_tag="PLV77_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC14505.1"
                     /translation="MSSALTLSPKQKSPNGQPCCEPVVYPDVERAEAERMAAIAKALG
                     DPIRLTLVDVLRKHAGKVCVCELVPLFDVSQSTLSHHLKKLRDAGIVDSERQGLWAYY
                     YVNPEELDELSAWLS"
     gene            804..1829
                     /locus_tag="PLV77_01255"
     CDS             804..1829
                     /locus_tag="PLV77_01255"
                     /inference="COORDINATES: protein motif:HMM:NF033735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNC14506.1"
                     /translation="MTDKLRIGINGFGRMGRLALRAGWDTEELEFVQINEIAGGPESA
                     AHLLEFDSVQGRWRREGIEWLEDSIRVAGKELAYTSGSDPGGVDWGKDEVDLVIEATG
                     KFRTPELLAPYFGRGVKRVLVAAPVKDDALNIVYGVNDSDYDPDRDRIVTAASCTTNC
                     LAPIVKVVHEGIGIRHGSITTLHDLTNTQVVVDAHHKDLRRARSAGTSLIPTSTGSAT
                     AIGMIFPDLVGRLDGLAVRVPLLNSSITDCCFEVDRATSVEEINDLLRTAASEELGGI
                     LGFETRPLVSVDFKGDPRSGIVDGTSTRVIDQTQVKILAFYDNEWGYANRMIDIARMI
                     ARSSGGD"
     gene            1833..3092
                     /gene="arsJ"
                     /locus_tag="PLV77_01260"
     CDS             1833..3092
                     /gene="arsJ"
                     /locus_tag="PLV77_01260"
                     /inference="COORDINATES: protein motif:HMM:NF033734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HNC14507.1"
                     /translation="MAGTARREGDLRNYVLVTAAYWADTLADGASRILVLFFFYQLGY
                     SALEVATLFLFYEFFGIVTNLVGGWIASRLGLKATLFSGLTIQIVALGMLGFAPDEWL
                     VVPYVMVSQALSGIAKDLTKMSSKSAVKLVVPEDSESSLFKWVAILTGSKNALKGVGF
                     FLGGLLLTLIGFQASMLVLLGIVATALITTASMMRGSLGYADMGAKFRHMFSNTREIN
                     VLAAARLFLFASRDVWFVVALPVYLRTVLDRSFWQAGGFLAIWVIGYGIVQASAPGMF
                     ARRSAETGATPTGRSAAKLAFLLAAFPAAIAASLAANLDPTLVLVTGLILFGIVFALN
                     SAVHSYLVLAYTDDESVAKNVGFYYMANAGGRLIGTILSGAVYQWLGLEGCLWFSVAF
                     VLTAATISLRLPSHNPKGAGAMPAAAG"
     gene            complement(3115..>4411)
                     /locus_tag="PLV77_01265"
     CDS             complement(3115..>4411)
                     /locus_tag="PLV77_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14508.1"
                     /translation="VIASLNGKSVKTVFTLPLHRRGDLKQDLATYREPIDIGVSPDGK
                     RLLITVSDGYSYDRKWLVLVNLNNGKQRMIGKNTRSGSFSPDGSRFAYVSSTGNSGET
                     CIETDETSCVIQGDLFVQNLDGSGKRRLTRSRAAEENPDWSPDGKRIAFDANYNLPGS
                     GAASEVYAIAPNGTCLSWLTNGSPASVEPDWAPEAGPSDLLCGIEPRETVISSDWIPT
                     KEEGAEISVWAGPVLGNRLVSEAVLTGPFRATVYDDCAVFNARACAEPAKYEIGGLST
                     CYAGLYLGIVVGEAKRSDFAHRRGVAFLREKRRTRRGNLVRSVTVFTGDTMLAIRGRG
                     NASFADLEKQIDGLRPATASVSAGRLPQLYLPKGMPNTVKFVAGMVGRIGVRRTSIRL
                     EADPKDIRTLVRFSSRLNLLGPIKTKTCTKEETDPFAGY"
BASE COUNT          762 a         1400 c         1373 g          876 t
ORIGIN      
        1 cgagaacggt ctgaccttct tcgagatcag cgacggcagt cggatttccg cagccgagcg
       61 aggcgagctg tgcagcttcg ggcagttcgc ttcggtcgcc ttcttcgtag aggttcgatc
      121 cgaatactgc ccgctgctct tcggtgatga ccgtgtcatt cgcgcagcag gacgcatccc
      181 gggagccggc gtcaactgcc gccgccgcgt acctctctcg cacgatctcc ctgagttcgg
      241 attcgatgcg ttcacttcct tcctggcacc cgcaaccctg ctcttcaccg caacactcat
      301 ccctggcttc ggcttcgcag caggaagcct gggcctccgg ggtgcaacag tcgttcacct
      361 gctctagctc agccatgcgc tcaactcgtc cagctcttcg gggttcacgt agtaataggc
      421 ccacagtccc tggcgttctg aatcgacgat tccggcatcc ctcagcttct tcagatgatg
      481 ggagagcgtc gactggctga cgtcgaacag gggtacgagc tcgcagacgc agaccttgcc
      541 ggcgtgtttc cgaagtacgt cgaccagtgt gagccggatc gggtcgccaa gtgcctttgc
      601 gatcgcggcc attcgttcgg cttcggcgcg ttcgacgtcc gggtagacga ccggctcgca
      661 acaaggctgg ccgttcggac tcttctgttt ggggctgagg gtcagtgcgg agctcatcga
      721 tattcaccaa atcagttcaa ttgacgtttg tcaatagata cgatagtcat cgatagatca
      781 aacgaggccc gggagggcga cggatgacag acaagctgag aattggaatc aacggcttcg
      841 gaaggatggg gaggctcgct ctgcgagccg gctgggacac cgaggaactc gaattcgtcc
      901 agatcaacga gatcgccggg ggccccgagt ctgcggcgca cctgcttgag ttcgactcgg
      961 tgcagggacg ctggcgccgg gaaggaatcg agtggctgga agactcgatc cgggtcgccg
     1021 gcaaggaact tgcctacacc tcggggagcg atcccggggg agttgactgg ggcaaggacg
     1081 aagttgatct ggtgatcgag gccaccggaa agttccgcac accggaactc ctggctccct
     1141 acttcggccg gggcgtgaag agagtccttg tcgcggcacc ggtcaaagat gacgcgctga
     1201 acatcgtcta cggcgtgaat gactccgatt acgaccccga cagggatcgc atcgtcaccg
     1261 ccgcctcctg cacgacgaac tgcctggccc cgatcgtcaa ggttgtccac gagggcatcg
     1321 gcatccggca cggatcgatt accaccctcc acgacctgac caacacgcag gtcgtggtcg
     1381 acgcccacca caaggacctc cggcgcgcga ggtcggccgg tacttcgctg atcccgacct
     1441 caaccggatc ggcaaccgcg atcggaatga tcttccccga tctcgtcggg cgactggacg
     1501 ggctggcggt gcgagtgccg ctcctcaact cctcgatcac cgactgctgc ttcgaggtcg
     1561 accgtgcaac ttccgttgaa gaaatcaatg acctgctccg gacggccgcc agcgaggagc
     1621 ttgggggaat cctcggcttc gagacgaggc cactggtttc cgtcgacttc aagggtgacc
     1681 cccggtccgg catcgtggac ggaacctcga cccgggtgat cgatcagacc caggtcaaga
     1741 tcctcgcctt ctacgacaac gagtggggat acgcgaaccg gatgatcgac atcgccagga
     1801 tgatcgcccg aagctccggc ggagactgag cgatggccgg caccgcgcga agagaaggtg
     1861 atctcaggaa ctacgtcctg gtcacggccg cctactgggc cgacacgctc gccgacggtg
     1921 ccagccgcat cctcgtcctg ttcttcttct accagctcgg ttacagcgcg ctggaagtcg
     1981 cgacactgtt cctcttctac gagttcttcg ggatcgtcac caacctggtc ggaggatgga
     2041 ttgcctcccg gctcggattg aaggcgacgc ttttctccgg gctgaccatc cagatcgtcg
     2101 cgctcgggat gctcggtttc gcgcccgacg agtggctggt cgttccgtac gtgatggtct
     2161 cccaggcgct ctccggcatc gccaaggacc tgaccaagat gagctcgaag agtgcggtca
     2221 agctcgtcgt cccagaggac tccgaatcat ctctcttcaa atgggtggcg atcctcaccg
     2281 gttccaagaa tgccctcaag ggcgtcggct tcttcctcgg cgggctgctg ctcaccctga
     2341 tcgggttcca ggcctcgatg ctggtattgc tcgggatcgt cgccaccgcc ctgattacca
     2401 ccgcgtcgat gatgcggggc agtcttggct atgcggacat gggggcgaag ttccgtcaca
     2461 tgttttccaa cacccgggag atcaacgtcc tcgcggctgc ccgcctcttc ctcttcgcct
     2521 cccgggatgt ctggttcgtc gtcgctctcc cggtctacct gaggacggtc ctcgaccgga
     2581 gtttctggca ggccggtggc ttcctggcaa tctgggtgat cggttacggg atcgtccagg
     2641 cgagcgctcc cggaatgttc gccaggagaa gcgcggagac gggagccact ccgaccggcc
     2701 ggtccgcggc gaaacttgcc ttccttctcg ccgccttccc tgcggcgatc gctgcgtcgc
     2761 ttgccgcgaa tctagatccg accctggttc tggtcaccgg cctgatcctg ttcgggatcg
     2821 tcttcgcgct caattcagcc gtgcactctt acctcgtgct cgcatacacc gatgacgaat
     2881 cggtcgccaa gaatgtgggc ttctattaca tggcaaacgc tggcggcagg ctgatcggaa
     2941 cgatcctctc cggagccgtc tatcagtggc tcggcctgga aggatgcctc tggttttcgg
     3001 tcgcgttcgt gctcaccgcc gcgacgatct cgctgcggct tccatcgcac aacccgaaag
     3061 gcgccggggc aatgccggcc gccgcgggct agcaaggcat cccggtccgc ccgttcaata
     3121 accggcgaag gggtctgttt cctctttggt gcaggtcttg gtcttgatcg ggccgagcag
     3181 gttcaggcgg ctcgagaatc tgaccagggt ccggatgtcc ttcgggtcgg cctccagcct
     3241 gatcgaggtt cgcctgactc cgatccggcc gaccatcccg gccacaaact tgaccgtgtt
     3301 cggcattccc ttcggcaggt agagctgcgg caaacgcccg gccgacacgg aagcggtggc
     3361 cggtcgaagc ccgtcgatct gcttctcgag atcggcgaag ctggcgttcc ctcggccgcg
     3421 aatcgcaagc atggtgtcac cggtgaagac cgtgacgcta cggaccagat tcccgcgcct
     3481 agtgcgccgc ttttcacgaa ggaaggcgac gccccggcga tgggcgaagt cggatcgctt
     3541 cgcttcgccg accacgatgc cgaggtaaag acccgcgtag caggtggaaa gcccgccgat
     3601 ttcatacttc gcaggctcgg cgcaggccct ggcgttgaag accgcgcaat cgtcgtacac
     3661 agtggcgcgg aagggacccg tcaggacggc ctcggagacc aggcgattgc ccagcacggg
     3721 accggcccag acgctgatct cggcaccctc ctccttggtg gggatccagt cactggaaat
     3781 caccgtttcc cttggctcga ttccgcaaag gagatccgaa gggccggctt cgggagccca
     3841 gtccggttcg actgacgccg gcgaaccgtt ggtgagccac gaaaggcagg tcccgttcgg
     3901 agcgatcgcg tagacctcgg aggccgcgcc cgatccgggg aggttgtagt tggcgtcgaa
     3961 agcgatccgc ttgccgtcgg gggaccagtc cgggttttcc tccgcggcgc gactccgggt
     4021 cagcctccgc ttgccgctgc cgtcgaggtt ctgaacaaac aggtcaccct ggatgacgca
     4081 gctcgtttca tcggtttcga tgcaggtttc accgctgttg cccgtcgacg agacataggc
     4141 gaagcggctc ccgtcgggtg agaaggagcc ggaccgcgtg ttcttgccga tcatgcgctg
     4201 cttgccgttg ttcaggttga ccagcacgag ccacttccgg tcgtagctgt agccgtcgct
     4261 caccgtgatc agcaaccgct ttccgtcggg ggaaacaccg atgtcgatcg gttcgcggta
     4321 ggtggcgagg tcctgcttca ggtctccccg gcggtggagc ggcagcgtga agaccgtttt
     4381 caccgacttg ccgttgaggc tggcgatcac g
//
LOCUS       DAOQMF010000044         4362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_9716_length_4362_cov_3.320641, whole genome shotgun sequence.
ACCESSION   DAOQMF010000044 DAOQMF010000000
VERSION     DAOQMF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9716_length_4362_cov_3.320641"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PLV77_01270"
     CDS             <1..306
                     /locus_tag="PLV77_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14509.1"
                     /translation="VGQPNLVRGFSGEPVPDPSMPILKRQKIVVNGRAGFPGLPLLLR
                     VGSLDSAYLAKTMNPILASNDPFVLQLVFEEPVTKRRIILVEVNQLVDDLGIVPVPI"
     gene            complement(429..716)
                     /locus_tag="PLV77_01275"
     CDS             complement(429..716)
                     /locus_tag="PLV77_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC14510.1"
                     /translation="MPKPFPREFRDDVVAVARTGHAPIKQVAKDFGISEACLHNWLKK
                     AEIEEGTRPGLTEAERKELRETKKRIRLLEQENEVLRRAAAYLSQANLPGK"
     gene            821..942
                     /locus_tag="PLV77_01280"
                     /pseudo
     CDS             821..942
                     /locus_tag="PLV77_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611484.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1243..3546)
                     /locus_tag="PLV77_01285"
     CDS             complement(1243..3546)
                     /locus_tag="PLV77_01285"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNC14511.1"
                     /translation="MPAAGTVPGNGAGIGSAAGPSAIETADLTARERSLLDDLMAVIQ
                     QFEADLATHGSSPAATIDRDEVTRAFVFACRHHADQKRKSGDEFITHPVGVARICVGM
                     ALDTETLCAALLHDTVEDTSASLEEIELEFSPTVSRLVDGVTKLTEITFESRDERQAE
                     NYRKMMVAMATDVRVILIKLADRLHNMRTLSSLPKQKQILKSRETLEIYAPLAHRLGI
                     HAIKWELEDLAFATLHPRKYSEIKHLVAQQRTERENFVEEAGEYLSDELEKVGIEAEI
                     AGRAKHFYSIYTKMTKKGREFNEIFDLTAMRVIVGSVKDCYGAIGVIHSLWKPLPGRF
                     KDYVAMPKANMYQALHTTVIGLEGKPLEIQIRTAEMHRLAEYGIAAHVAYKEGGSGTD
                     SSREKMTWLRQLVESESEQDPTEFLQSLKEDLFEDEVFVFTPKGEVMNLSAGSTPVDF
                     AYSIHTDVGHRCVGAKVNGKIVPLHYQLKSGDIVEVLTANKDRGPSRDWLKLVGTSRA
                     RNKIRAWFREESREDAERDGREALAEALRKRDLPPHKFSGSPMLADVMREMGFRKAED
                     FYIALGQGKVSARTTANKLIQRLRQGEIIEPAPVENIESGTPKRSRALREASEFGIKV
                     EGLENVALRLAKCCRPVPGDEIVGYVSLGRGITIHQANCQNVADLEKSPERMIDVAWE
                     GENEATFRVEIQIDAYDRTRLLEDLSRTFSEAGVNIISATCTTNPPMVKNRFVVEVGD
                     TGQLKQCINRIRHVESVFDAHRVNTTD"
     gene            complement(3579..>4362)
                     /locus_tag="PLV77_01290"
     CDS             complement(3579..>4362)
                     /locus_tag="PLV77_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14512.1"
                     /translation="LEGAGPAEKAEVRIASGPLGLPGVALGELISSGERIVVLTADAR
                     QRWVGLGGAALGRFSSRDGEEAVGVWAGSPASVSEPARDASVLLTDFTTLAGANPPVI
                     DDFDRIALLDPPYLAASVAELARLDLPVHLLAGPVEFEFTARVAAYHLDPTVQLRNLF
                     RRFREADAIDGQATEGEPLRAVLSEDGNSTRSPEQAATLLRILVETGLARTEGEGSAR
                     RAGVVSSVKADLSVSVVFSHHIELHQEHRGFLSQFDRQTQTQ"
BASE COUNT          766 a         1397 c         1370 g          829 t
ORIGIN      
        1 gtcggtcaac caaacctggt tcggggcttt agcggtgaac cggtgccgga tccgtccatg
       61 ccgatcctca aacgccagaa gatcgtcgtg aacgggcggg ccgggtttcc cggccttccc
      121 cttcttcttc gcgtgggaag cctggattcc gcctatctgg caaagacgat gaacccgatt
      181 ctcgcaagca acgatccctt cgtgctccag ctcgtctttg aggaaccggt aaccaagcgt
      241 cggatcatcc tcgtggaggt caaccagctt gttgatgacc tcggcatcgt cccggtcccg
      301 atctgaaaca ggctctttga gccacttgta atacccctgg gagctgaacc caaggacccg
      361 gcacgtcacc ttgaccggca cccggatcgg ggcaccagcc aaagccattt cacggacgag
      421 cgggaagatc attttcccgg cagattagcc tgggaaagat acgcggccgc ccgtctgagc
      481 acctcgtttt cctgctccaa caggcggatc cgcttttttg tttcgcgcaa ctctttgcgc
      541 tcagcctcgg tcaaaccagg ccgggttccc tcctcgatct cggccttctt caaccagtta
      601 tgcaggcacg cttcggagat cccgaagtct ttggccacct gcttgatcgg cgcgtggccc
      661 gtacgggcca cggcaacaac atcatcacgg aactccctcg gaaacggctt cggcatgacg
      721 gacatccttc cactgagaga aaatcctcag acttgaagtg tcaaccaaac ccgggccaga
      781 cccaatcgac cgcaacggcc cctggaagaa atgctccgac gttgaactgg cagccctgac
      841 ctgggtgact ggtacaaccg atcaaggctc catggagccc tgaaatacct gccgcccgta
      901 gagttcgaga acaactacct gacggcacca accgcccaat gaaaaccgtc tccatcaaac
      961 ccggaatgga tcaagatatg aagtggacgc actctggaag cgtgagcggc tggtggtcga
     1021 gctggacagc cgaagattct acggtgatgc tttcgctctg gaagccgatt ccgagaagac
     1081 tcgctacctc cgcagcctcg gctacaaagt cctgagattc acctggttgg acgtaactga
     1141 acgtccggag tgggtcgcgg cctgcatccg cgaggcactg agtgcggatt aacctcgtag
     1201 tacagggtta atcgaccaca ggcccttgga accgggaagt ggtcagtccg tggtgtttac
     1261 gcggtgggcg tcgaagacgc tttcgacgtg gcggatccgg ttgatgcatt gcttgagctg
     1321 gccggtatct ccgacttcca ccacgaaacg gttcttgacc atcggcgggt tggtcgtgca
     1381 ggtggccgag atgatgttga ccccggcctc cgagaaggtg cgggaaagat cctcgagcag
     1441 gcgggtccgg tcgtaggcgt cgatctggat ctcgacccga aaggtcgcct cgttctctcc
     1501 ctcccaggcg acatcgatca tccgttccgg tgacttctcg agatcggcca cgttctggca
     1561 attggcctgg tggatcgtga tgccacggcc gagcgacaca taaccgacga tctcgtcgcc
     1621 ggggaccggc cggcaacact tggcgagccg gagcgcgacg ttttcgagcc cctcgacctt
     1681 gatcccgaac tcggatgcct cgcggagcgc gcggctgcgc ttcggggtgc cggactcgat
     1741 gttctcgacc ggggcgggtt cgatgatctc gccctgcctg aggcgctgga tcagcttgtt
     1801 ggcggtggtc ctggccgaga ccttcccctg gccgagggcg atgtagaaat cctcggcctt
     1861 gcggaagccc atctcccgca tcacatcggc cagcatcggg gaaccggaga acttgtgcgg
     1921 cggcaggtcc cgcttgcgca gcgcttcggc cagtgcctcg cggccatccc gctcggcgtc
     1981 ctcgcggctt tcctcgcgga accaggcgcg gatcttgttg cgggcgcggc tggtcccgac
     2041 cagcttcagc cagtcccggg aagggccgcg atccttgttc gcggtcagga cttcgacgat
     2101 gtcgcccgac ttcagctggt aatggagcgg cacgattttg ccgttgacct tcgccccgac
     2161 gcagcggtgg ccgacatcgg tgtgaatcga atacgcgaag tcgaccgggg tcgagccggc
     2221 cgagaggttc atcacctcgc ccttcggagt gaagacgaat acctcgtcct cgaagagatc
     2281 ctccttcagc gactggagaa actcggtcgg atcctgctcg gactccgact cgaccagctg
     2341 ccgcagccag gtcatctttt cgcgggagga atcggtgccg ctgccgcctt ccttgtaggc
     2401 gacatgggcg gcgatcccgt actccgccag acgatgcatt tccgccgtcc ggatctggat
     2461 ctccagcggc ttgccctcaa ggccgatcac cgtcgtgtga agtgcctggt acatgttcgc
     2521 cttcggcatc gcgacgtaat ccttgaagcg accgggcagg ggcttccaga gcgaatggat
     2581 caccccgatc gcgccgtaac aatccttgac cgagccgacg atcacccgca tcgcggtcag
     2641 gtcgaagatc tcgttgaact cccggccctt cttggtcatc ttcgtgtaga tcgagtagaa
     2701 gtgcttggcg cggccggcga tttcggcttc gatgccaacc ttctccagct cgtccgacag
     2761 gtactcgccc gcctcctcga cgaagttctc gcgctcggtc cgctgctggg cgaccaggtg
     2821 cttgatctcc gagtacttgc gcggatgaag cgtcgcgaag gcgaggtcct cgagttccca
     2881 cttgatcgcg tggataccga gccggtgggc cagcggcgcg tagatctcca gcgtctcgcg
     2941 ggacttgagg atctgcttct gcttgggcag cgaactcagg gtccgcatgt tgtgaaggcg
     3001 gtcggcgagc ttgatcagga tcacccgcac gtcggtcgcc atcgccacca tcatcttgcg
     3061 gtagttctcg gcctgcctct cgtcacggct ttcgaaggtg atctcggtca gtttggtgac
     3121 gccatcaacc aggcgggaaa cggtcgggct gaactccagc tcgatctcct cgaggctggc
     3181 cgaggtgtcc tcgaccgtgt cgtggagcag ggccgcgcag agtgtctcgg tgtcgagcgc
     3241 catgccgacg cagattcggg cgaccccgac cggatgggta atgaactcgt cgccggattt
     3301 acgcttctgg tcggcgtggt gccggcaggc gaagacgaag gcgcgagtca cttcgtcccg
     3361 gtcgatggtc gcggcgggag atgacccgtg ggttgcgagg tcggcctcga actgctggat
     3421 gaccgccatc aggtcgtcga gcagggatcg ttcgcgagcg gtcagatcgg cagtctcgat
     3481 cgccgacggg ccggctgcgg agccaatgcc ggctccgttt ccgggcacgg ttccggctgc
     3541 gggcacgacc ggaccgaccg gcggcgtcgc ctccggtgtc actgcgtctg ggtctgcctg
     3601 tcgaactggc tgagaaatcc tctgtgctcc tgatgaagct cgatgtgatg tgagaaaacg
     3661 accgaaacgg aaagatccgc cttcaccgag gatacgaccc cggcccttcg ggcactcccc
     3721 tcgccttcgg tacgggcgag gcctgtctcg accagaatcc gcagcagagt cgccgcctgc
     3781 tccggcgaac gggttgagtt gccgtcttcg gagaggaccg cccggagggg ctccccttcg
     3841 gtcgcctgac cgtcgattgc gtcagcttcg cgaaagcggc ggaagaggtt cctgagctgc
     3901 acggtcgggt cgaggtgata cgccgcgacc cgggccgtga actcgaactc gaccggcccg
     3961 gcaagaagat gaaccggcag gtcgagcctg gccagttccg ccacgctcgc ggccagatac
     4021 ggcgggtcga gcagggcgat gcggtcgaag tcgtcgatca ccgggggatt ggcgccggcc
     4081 agggtcgtga agtcggtgag caggaccgac gcatcgcggg ccggctccga aacggatgcc
     4141 gggctgccgg cccagacccc gaccgcctcc tccccgtcgc gtgaagaaaa gcggcccagg
     4201 gcggcgccgc cgagaccgac ccagcgctgg cgcgcatcgg ccgtgagcac gacgatccgc
     4261 tctccggagc tgatcagctc gccgagcgcc acacccggca ggccgagcgg ccccgaggcg
     4321 attctgacct ccgccttttc agccggcccg gctccctcca gg
//
LOCUS       DAOQMF010000045         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_9725_length_4360_cov_3.384669, whole genome shotgun sequence.
ACCESSION   DAOQMF010000045 DAOQMF010000000
VERSION     DAOQMF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9725_length_4360_cov_3.384669"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLV77_01295"
     CDS             complement(<1..404)
                     /locus_tag="PLV77_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14513.1"
                     /translation="MIEPRALERIALTRLPELQVASGLFRATARIEGVEDETAPATTL
                     QAGIVVLLGLLRAEEAETDQPFSTGALRTRILGDLSGPDVTAGELGLALWAESRCGGE
                     AIGEIDGLIRRATVGGLEKVPTEQIAWLASGLA"
     gene            complement(513..2321)
                     /locus_tag="PLV77_01300"
     CDS             complement(513..2321)
                     /locus_tag="PLV77_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNC14514.1"
                     /translation="MQTSETVTEFDGGPAVEPEFKNLYEAFQATVARKGDGLAIKSEA
                     EEVELSWNDLDKRVRAIAGGLHALGIQRGDRVGMLLGARSDFIPIDLAVVSIGGLPFS
                     IYQTLAPDQIEYVVEDSGTELIITEEAYLPNLLEARKNLPNLKRVVMLDGEGGDMTMA
                     ELEAMDPEFDPAPLAAEVGLDDPLTLIYTSGTTGPPKGVLLTQGNLMSMLTTVMHSVL
                     EIPESGRVISWLPAAHIAERGANYYTPVMHGLEVHVCPDPKRIIEFLPKVRPSWFFAV
                     PRIWEKLKAGLESNLASLPEEQREKAQAGLQAAIQKVRLEQAGEEVPEELAAAVAAAD
                     EALFSHLRVALGFDEALAVSAGAAPTPAEVLEFFHAIGIPVGELWGLSETCGVITINP
                     PKKIKIGTVGPPVPGVEMRVAEDGELLCRSPFVMKEYRNKPEKTAETIVDGWLLTGDI
                     GEIDEEGYVTILDRKKELIINAAGKNMSPANIEAQIKVSSPLINQAVTIGDSRPYNVA
                     LIVLDPDFAPVWASQNGIEGSFEDLAGDERIEAAIQAAVEEANGKLARVEQIKKFKLI
                     PGEWLPGGDELTPTMKLKRKPIAEKYESEIEDLYST"
     gene            complement(2500..3567)
                     /locus_tag="PLV77_01305"
     CDS             complement(2500..3567)
                     /locus_tag="PLV77_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNC14515.1"
                     /translation="MSFLVAFLAFSALIVLHEAGHFTAAKAVGMRVERFFLFFPPKLV
                     SIKRGETEYGIGAIPLGGFVRITGMNPDEELPPEIAARGYYNQPVWKRIVVIGAGPAV
                     NIVLAFLILWVLAFGIQQPSQNVEQIQPGSPAAAKLQAGDRIISVDGVSGSQEKIADE
                     IAAHQCPGKQTDGCKAAEPATVVVMRDGQEKTLRITPVYDASVQRSRLGFAFGLEDAN
                     PGPVEAARISADYMWFVTSSTVSALSRLYEKEQRDQLSGVVGSYEVTRQAVDVGAREA
                     LTLIALISLSLGIINLFPFLPLDGGHIFWSLVEKVRGRPVPFAVMERAGLIGFALVIG
                     LFILGLNNDIGRLTGEGFNVR"
     gene            complement(3597..>4360)
                     /locus_tag="PLV77_01310"
     CDS             complement(3597..>4360)
                     /locus_tag="PLV77_01310"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNC14516.1"
                     /translation="ELVTELASAAGAKLLPVDSEHSALFQLVENEDRAAIDRIVLTAS
                     GGPFRGCTDLAGITVEQALDHPTWAMGGRITIDSATLMNKGFEMIEAHHLFDLPYEQI
                     AVVVHPQSIVHSLIDLRDGATLAHLGYPDMRVPIAYALTWPERAEVPVERLDLAKVGS
                     LDFEAPDPDTFRCLALAREAGESGGIAPCVLNAADEVAVAAFLEGKVSFIDIARVIEQ
                     TLDDIGSGPAKGFEELFAVDEASRERASEIISGLN"
BASE COUNT          754 a         1421 c         1392 g          793 t
ORIGIN      
        1 gccagaccgg aggcaagcca ggcgatctgc tcggtcggaa ccttctccag gcctccgacc
       61 gttgcccgcc ggatcaagcc atcgatttcc ccgatcgctt ctccgccgca cctcgattcg
      121 gcccagagcg cgagtccgag ttcaccggcg gtcacgtccg gaccgctcag gtcacctagg
      181 attcgggtcc tcagcgcgcc ggtggaaaag ggttggtcgg tctcggcctc ttcggcccgc
      241 aacagcccga ggagcaccac gatcccggcc tgaagcgtcg tcgccggagc cgtctcatcc
      301 tccaccccct caatccgcgc agtagcccga aaaagcccgg aagcaacctg cagctcaggc
      361 aaccttgtaa gtgcgatgcg ctccagggcg cgcggttcga tcatctataa gccgaggtta
      421 tatgcgctcg aagcatctgg accggtgccg ataggtccct gacgacgttt cgggccgagg
      481 aaggggccta tcggtaccgg tcccggagac tctcaggtgg agtagagatc ctcgatctcg
      541 gattcgtact tctcggcgat cggtttccgc ttcaacttca tggtcggcgt cagttcatcg
      601 ccacccggca accattcacc cgggatcagc ttgaacttct tgatctgctc gacccgggcc
      661 agtttgccgt tggcttcctc gaccgcggcc tggatggccg cttcgatccg ctcgtcaccg
      721 gccaggtcct cgaaggaacc ctcgatcccg ttctgggagg cccagaccgg agcgaagtcg
      781 gggtcgagca cgatcagcgc gacgttgtag gggcggctgt ctccgatcgt caccgcctgg
      841 ttgatcaggg gtgaagagac cttgatctgc gcctcgatgt tggccggtga catgttcttg
      901 ccggccgcgt tgatgatcag ctccttcttg cggtcgagga tcgtcacgta accctcttcg
      961 tcgatctcgc cgatgtcacc ggtcaaaagc cagccatcga cgatcgtctc ggccgtcttc
     1021 tcgggcttgt tgcggtactc cttcatcacg aaggggctgc ggcagagcag ctcgccatcc
     1081 tcggccaccc gcatctcgac cccgggaact ggtggtccga ccgtgccgat cttgatcttt
     1141 ttcggcgggt tgatcgtgat cacgccgcag gtctcggaga ggccccagag ctcgccgacc
     1201 gggatgccga tcgcatggaa gaactcgagc acctcggccg gggtcggtgc agctccggca
     1261 cttacggcca gcgcctcgtc gaagcctagg gcgacccgca ggtgcgagaa gagcgcctcg
     1321 tcggccgctg cgaccgcggc ggccagctct tccggcacct cttccccggc ctgttccagc
     1381 cgtaccttct ggatcgccgc ctgaagtccg gcctgggcct tttcgcgctg ctcttccggc
     1441 aaggaggcca gatttgattc cagtccggct ttcagcttct cccagatccg ggggacggca
     1501 aagaaccagg agggccgcac cttgggcagg aactcgatga tccgcttcgg gtccgggcag
     1561 acgtggacct cgagaccgtg catgaccggc gtgtagtagt tggcgccccg ttcggcgatg
     1621 tgggcggccg gcagccagga aatcacccgg ccgctttcgg ggatctcgag caccgaatgc
     1681 atgaccgtgg tcagcatcga catcaggttg ccctgggtca gcagcacacc cttgggcgga
     1741 ccggtggtcc cggaggtgta gatcagagtg agcgggtcgt cgagcccgac ctcggccgcc
     1801 agcggggccg gatcgaattc gggatccatg gcttccagtt ccgccatggt catgtcgcca
     1861 ccttcaccgt cgagcatcac gacgcgcttc aggttcggca ggttcttgcg ggcctcgagc
     1921 aggttgggca ggtaggcctc ctcggtgatg atcagctcgg tcccggaatc ctcgaccacg
     1981 tactcgatct ggtcgggggc gagggtctgg tagatcgaga agggcaggcc gccgatcgaa
     2041 accaccgcga gatcgatcgg aatgaagtcg gaccttgctc ccagcagcat tccgacccgg
     2101 tcgccgcgct ggatgccgag cgcgtggaga ccgccggcga tcgcgcgaac ccgcttgtca
     2161 aggtcgttcc agctgagttc aacctcttcg gcctcggact tgatggccag cccgtcaccc
     2221 ttgcgggcga cggtcgcctg aaaggcctcg tagaggttct tgaattctgg ttcaaccgcg
     2281 gggccaccgt caaattcggt caccgtctcg gacgtctgca cctgctcctc cttcgctctc
     2341 ttcccaaaaa gctacgccgg gaatgtatcc caaccagagc aacgggcata taggggcaac
     2401 cccctaggcc aaccaaccaa cggagctcgc ccatctatcc gttgcctccg tgggcgagcg
     2461 ggttctacat ggagggcggg tccgaccccg cccgactcct tatctaacgt tgaacccctc
     2521 gcccgtcagg cggccgatgt cgttgttgag gccgaggatg aagaggccga tgaccagggc
     2581 gaagccgatc aggccggctc tttccatgac cgcgaacggg accggcctcc cccgcacctt
     2641 ttcgaccagg ctccagaaga tgtggccgcc gtcgagcggc aggaacggga agagattgat
     2701 gatgccgagc gagaggctga tcagggcgat cagggtcagc gcctcgcggg ccccgacgtc
     2761 gacggcctgc ctggtcactt cgtagctgcc gaccaccccg gaaagctggt ctcgctgctc
     2821 cttctcgtaa aggcgggaca gcgccgaaac cgtgctggag gttacgaacc acatgtagtc
     2881 ggccgagatt cgcgccgcct cgaccgggcc ggggttggca tcttcgagac cgaaggcgaa
     2941 gccgagacga ctgcgctgaa ccgaggcgtc atacaccggt gtgatccgga gcgtcttctc
     3001 ctggccgtcg cgcatgacca cgacggtggc cggctccgcc gccttgcagc cgtcggtctg
     3061 cttgccggga cactggtggg cggcgatctc gtcggcgatc ttctcctggc ttccgctcac
     3121 cccgtcaacc gagatgatcc ggtccccggc ctggagcttc gccgcggccg ggctgccggg
     3181 ctggatctgt tcgacgttct ggctcggttg ctggatgccg aaggcaagca cccagaggat
     3241 caggaaggcg agcacgatgt tgaccgccgg cccggccccg atcaccacga tccgcttcca
     3301 gaccggttgg ttgtaatagc cgcgggcggc gatttccggg ggcagttcct cgtccgggtt
     3361 catgccggtg atccgcacga aaccaccaag cgggatcgcc ccgatcccgt attcggtctc
     3421 cccccgcttg atcgaaacca gcttcggcgg gaagaagagg aagaaacgct caacccgcat
     3481 tcccaccgcc ttggcggcgg tgaagtgccc ggcctcgtgg aggacgatca gggcactgaa
     3541 agcgaggaat gcgacaagaa agctcatgag aagtcagggc cggccggcgg gccgattcag
     3601 ttcagaccgg aaatgatctc agaagcgcgt tcccggcttg cttcgtccac cgcgaaaagc
     3661 tcttcaaagc ctttggcggg gccactcccg atgtcatcga gggtctgctc gatcaccctg
     3721 gcgatatcga tgaaggagac cttgccttcg aggaacgcgg ccaccgcgac ctcatcggca
     3781 gcgttcagga cgcaaggggc gattccgccg gattcccctg cttcccgggc aagggccagg
     3841 cagcggaagg tgtccggatc gggtgcttcg aaatcgaggc tgcccacctt cgcgaggtcg
     3901 aggcgctcga ccggaacctc agcccgctcc ggccaggtca gggcgtaggc gatcggcacc
     3961 cgcatgtccg ggtaaccgag gtgggccagg gtggcgccgt cacgcagatc gatcagcgaa
     4021 tggacgatcg actggggatg gaccaccacg gcgatctgct cgtagggaag gtcgaacagg
     4081 tgatgggcct cgatcatctc gaagcccttg ttcatcaggg ttgccgagtc gatcgtgatc
     4141 cggccgccca tcgcccatgt cggatggtct agagcctgct cgacggtgat tcctgctagg
     4201 tcggtgcagc cacggaaggg accgccggaa gcggtaagga cgatgcggtc gatcgcggcc
     4261 cggtcctcgt tttcgaccag ctggaaaagg gcggaatgct cggaatcgac cggcaggagc
     4321 ttcgcaccgg cggccgaggc aagctcggtg accagctccc
//
LOCUS       DAOQMF010000046         4342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_9797_length_4342_cov_3.367157, whole genome shotgun sequence.
ACCESSION   DAOQMF010000046 DAOQMF010000000
VERSION     DAOQMF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9797_length_4342_cov_3.367157"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLV77_01315"
     CDS             <1..500
                     /locus_tag="PLV77_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14517.1"
                     /translation="VAAAQTLPLDTPYRAELVLIAYVGAAAMLLLQGSTLPWVINRFR
                     VQGDDPERIRNEYGDMINEISAAGLEELDRQAEGGRFSPEMIERVKRDSLIREGGARR
                     SSEDPDRDRQQYIDLRLAVLGAERDRMLHHRRVGTYSAEVIDRTQRILDLEEARLQQV
                     SGEPR"
     gene            complement(564..2036)
                     /locus_tag="PLV77_01320"
     CDS             complement(564..2036)
                     /locus_tag="PLV77_01320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNC14518.1"
                     /translation="MAELKQLTSLDAQFLAIETDRNYGHVGGLAILDPTTAPGGKLTI
                     EDLRQLIQDRLHLIAPFTQRLVSVPLSIDWPYWTQDEHFDIGYHCREIALPEPGSHEQ
                     LLEQVGRIYSRRLDRSRPLWEIYLIHGLEGGKVALLSKTHHAAIDGMSGAEILSALYD
                     LGPEPRKVDPPATAPDRSAPDGKDLLMMWVKGIPRSPLNLVDRLTTVVPHVDMAVAAF
                     GLPGSRRISRVMSRVRIRFGAEEEHVIERPPVKAPKGPYGAALSPHRVFGLGQVSLDD
                     VKTVKNAFGVKVNDVVICLVAGAVREHLTFTGELPEEPLLAMIPVSVRTEEERGTFGN
                     KVSAMIVPLPTHIDDPAERLEFIHSVMGAAKDSHDALPAEALRDLTDFVPPALHARAA
                     RVVTTVAGRFTRPPWNVIISNVPGPPIDLYFAGAKLEAMYPLSIISDGIGLNVTIMSY
                     RNSIDIGITADREQTPEVQTLVDGMQSELELLLAAARAAA"
     gene            complement(2038..2787)
                     /locus_tag="PLV77_01325"
     CDS             complement(2038..2787)
                     /locus_tag="PLV77_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14519.1"
                     /translation="MNDGANWLADTATAVADRLGLPHNRLFDEWASTLDPSRWPEPPE
                     GDGRPAMLIPGFLAGDPSLSNMARWLKRGDWNMTRSGINWNVNCTQATIEGVEARLRA
                     SVEASGKRALLVGQSRGGAMGKVIAVRNPDLVETLVTLGSPLNDHFNIHPRTWFGIGI
                     IGGLGELGFPGFLSRDCATGDCCKETRIAYGRPFPKDVRFISFYSRADTIVRYQACLH
                     QAAEHVEIESSHLGMGLDSEVWVRMSEELSR"
     gene            complement(2917..>4342)
                     /locus_tag="PLV77_01330"
     CDS             complement(2917..>4342)
                     /locus_tag="PLV77_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC14520.1"
                     /translation="LVLVMPGESTDLPVVQMGAAQIAAAIRAGELTSAEAVEASIARI
                     EEVNPRLNAVVTPTFESAREAAAAADRQIAAQGSDGLPPLFGVPITIKDCYPVDGVRF
                     TAGSWFHRDEVADGDAVAVERLRKAGAIILGKTNIPDMCWGFESVNPVFGRTESARHR
                     GYSAGGSSGGEASIIAAGGSALGLGSDIGGSLRNPAANNGCVSLKPTTGRVPDEGHVP
                     TVPPPVNAWNHAGPLARRVEDLALALGVLDGSGEADLPGVEGIRCRVFTGNRSMIVSP
                     EVKSAVRRAAGALGSAGMEVEQSPALPIQRMTMLYSHVLRKHALPEINAELGGGERFN
                     WLAEVRRTLTGRARISAEALSVYAYIAYGGVLKPGPGDADRKLERLRRQVIETVGDGV
                     LVCPLLLVRPARHGATWLPFTQIPMAVPFNITGLPVAMVPVYWTANGLPLSVQVVAGP
                     GKDELALAAARELEERLGGWKPID"
BASE COUNT          718 a         1436 c         1454 g          734 t
ORIGIN      
        1 ccgtagcggc cgcccagacc ctgccgctcg acaccccgta ccgggccgaa ctggtcctga
       61 tcgcctatgt cggcgcggcg gcgatgctgc tgctccaggg ttcgaccttg ccctgggtga
      121 tcaaccgctt ccgggtccag ggcgatgatc ccgaacggat ccgcaacgag tacggggaca
      181 tgatcaacga gatctccgct gccggtcttg aggagctcga tcgtcaggcc gagggaggcc
      241 gcttcagccc cgagatgatc gaacgggtca aacgcgacag cctgatccgt gagggcggcg
      301 cgaggcgttc ctcggaggat cctgaccggg accggcagca gtacatcgac ctgcgtctgg
      361 cagtgctcgg ggccgagcgc gaccggatgc tgcatcaccg gcgggtcggc acctacagcg
      421 ccgaggtgat cgacaggacc cagcgcatcc tcgacctcga ggaggcccgg ctccagcagg
      481 tctcgggcga gccgcgctaa ctccgttcct gcccagcccg tttcactcgt tgccccgcgc
      541 catcggcccg aacggggcgg gtctcaggcg gcggcgcggg ctgcggccag cagcagctcg
      601 agttccgact gcatgccgtc gaccagggtc tggacctcgg gggtctgctc acggtcggcg
      661 gtgatcccga tgtcgatcga attccggtag ctcatgatcg tcacgttcaa cccgatcccg
      721 tccgaaatga tcgagagcgg atacatggcc tcgagcttcg ccccggcgaa gtagaggtcg
      781 atcgggggtc cggggacatt cgagatgatc acgttccagg ggggccgggt gaagcgcccg
      841 gccacggtgg tgacgacccg ggcggcccgg gcgtgaagtg ccggtggcac gaagtcggtg
      901 aggtcacgca gagcctcggc cggaagggca tcgtgactgt ccttggcggc acccatgacc
      961 gaatgaatga actcgagccg ctcggcggga tcatcgatgt gggttggcag cggcacgatc
     1021 atcgccgaga ccttgttgcc gaaggtgccg cgctcttcct cggtccggac cgagacgggg
     1081 atcatcgcca gcagcggttc ctccggcagc tcgcccgtga aggtgagatg ctcgcggacc
     1141 gcccccgcga ccaggcagat caccacgtcg ttgaccttga ctccgaacgc gttcttgacg
     1201 gtcttcacgt catcgaggga aacctgtccc aggccgaaaa cgcggtgcgg cgaaagtgcc
     1261 gcgccataag gacccttcgg cgcctttacc ggcggccttt cgatcacatg ctcttcttcg
     1321 gctccgaagc ggatcctcac ccgggacatg acccggctta tccgccggct gcccgggagg
     1381 ccgaaggccg caaccgccat gtccacgtgc ggcaccacgg tggtcagccg gtcaaccagg
     1441 ttgagcggcg agcggggaat ccccttcacc cacatcatca gcaggtcctt gccgtccgga
     1501 gcggaccggt ccggagcggt cgccggcggg tccaccttcc gcggctcggg accgaggtcg
     1561 taaagggcgg agaggatttc ggcgccggac atgccgtcga tcgccgcgtg atgggtcttc
     1621 gaaagcagcg cgaccttgcc gccctcgagc ccgtggatca aatagatctc ccagagtgga
     1681 cgggaacgat cgagtcgccg cgaatagatg cggccgacct gctccagcag ctgctcgtga
     1741 ctgcccggtt ccgggagcgc gatctcccgg cagtggtaac cgatgtcgaa gtgttcatcc
     1801 tgggtccagt acggccagtc gatgctcaac ggaaccgaga ccagccgctg ggtgaacggc
     1861 gcgatcaggt gcagccggtc ctgaatcagc tgcctcagat cttcgatcgt cagcttgccg
     1921 ccgggagccg tggtggggtc gagaatcgcg aggccgccga cgtggccgta gttacgatcg
     1981 gtttcgatcg ccagaaactg ggcgtcaagt gaggtgagct gcttcagctc ggccatctca
     2041 gcggctgagt tcctcgctca tgcggaccca gacttccgag tcgaggccca tcccgaggtg
     2101 gctgctctcg atctcgacgt gctcggcggc ctgatggaga caggcctggt aacgcacgat
     2161 ggtgtcggcg cgcgagtaaa aggagatgaa gcggacatcc ttggggaacg gccggccgta
     2221 ggcgatccgg gtttccttgc agcagtcccc cgtggcacag tcacggctga ggaaaccggg
     2281 gaaaccgagt tcaccgagtc cgccgatgat gccgatcccg aaccaggtgc gggggtgaat
     2341 gttgaagtga tcgttcagcg gggagcccag ggtcaccagg gtctcgacca ggtcggggtt
     2401 gcggaccgcg atcaccttgc ccatcgctcc gccccggctc tggcccacca gcaaggcccg
     2461 tttgccgctg gcttcgaccg acgcccggag tcgcgcctcg acgccttcga tcgtggcctg
     2521 ggtgcagttg acgttccagt tgatgccgct cctggtcatg ttccagtcgc cgcgtttcag
     2581 ccagcgggcc atgttcgaaa ggctcgggtc accggcgagg aaaccgggga tcagcatcgc
     2641 cggccggccg tcaccctcgg gaggttcggg ccaccgggat ggatcgaggg tcgatgccca
     2701 ttcgtcaaaa agccggttgt gcggaagtcc cagccggtcg gcgaccgcgg tggcggtgtc
     2761 agccagccag tttgcgccgt cgttcacgga cggcagcgtt ggaacctttc caattgatgg
     2821 aaaccctccc agtggcaatt ccatgtgatg aatgttactc cccggcgcag cggaccagcc
     2881 atgtgttgcg ccccggaggc cgggttgcag actggctcag tcgatcggtt tccagccgcc
     2941 gagccgctcc tccagctccc tcgcggcagc cagggccagt tcgtccttgc ccgggccggc
     3001 caccacctga accgaaagtg gaagaccgtt cgcggtccag tacaccggca ccatcgccac
     3061 cggcaacccg gtgatgttga aagggaccgc catcggaatc tgggtgaatg gcaaccaggt
     3121 cgccccgtgt cgggccggtc ggaccagcaa aagcggacag acgagcacac cgtccccgac
     3181 cgtctcgatc acctgccggc gcagccgctc gagcttccgg tcggcatcac ctggaccggg
     3241 tttgagaacg ccgccgtagg cgatgtatgc gtagaccgag agtgcctcgg ccgagatcct
     3301 tgcccggccg gtgagggtgc gtcgaacttc cgcgagccag ttgaaccgct cgcctccccc
     3361 gagttcggcg ttgatctcgg gcaatgcgtg ctttctgagc acatgcgagt agagcatggt
     3421 catcctctgg atcggcaaag ccgggctctg ctcgacttcc attccggccg atccgagtgc
     3481 gccagccgca cgccggaccg cggatttcac ttcggggctg acaatcatcg agcggttgcc
     3541 ggtgaagacc cggcagcgga tcccttccac cccgggcagg tctgcttcgc cgctgccgtc
     3601 gagcacaccc agcgcgaggg cgaggtcttc gacccggcgg gccagtggtc ccgcatggtt
     3661 ccaggcattc accggtggcg gcacggtcgg cacgtggcct tcgtcgggca cccggccggt
     3721 ggtcggcttc agggaaacgc agccgttgtt cgcggcgggg ttccgcaggc tgccgccaat
     3781 gtcgctgccg agcccgaggg ccgaaccgcc ggcggcgatg atcgacgcct cgccgccgct
     3841 gcttcccccg gccgaatagc cacggtgcct ggcgctttcg gtgcggccaa agacgggatt
     3901 gaccgattca aagccccagc acatgtcggg gatgttcgtc ttgccgagga tgatcgcccc
     3961 ggccttcctg aggcgttcga cggcgaccgc gtcaccatcg gccacttcgt cacggtgaaa
     4021 ccaggaacct gcggtaaatc gcaccccgtc gaccgggtag cagtccttga tcgtgatcgg
     4081 gacgccgaac agcggtggca acccgtcgga gccttgcgcg gcgatctgcc ggtcggccgc
     4141 ggcggcggct tcccgggcgg attcgaaggt cggggtgacc accgcattca gccgcggatt
     4201 gacttcttcg atccgggcga tgctcgcctc gaccgcctcc gccgaggtca gctcgccagc
     4261 ccggatggcc gcggcgatct gcgccgcgcc catctggact accggtagat cggtgctctc
     4321 cccgggcatg actagaacca gg
//
LOCUS       DAOQMF010000047         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_9863_length_4323_cov_4.453608, whole genome shotgun sequence.
ACCESSION   DAOQMF010000047 DAOQMF010000000
VERSION     DAOQMF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9863_length_4323_cov_4.453608"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="PLV77_01335"
     CDS             <1..1265
                     /locus_tag="PLV77_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14521.1"
                     /translation="ITGAATTDGIAVDSSHIYWTLPGPGRIARANLDGSGANTSFIQN
                     AGNPVAVAVDSGVSPPTLTINSGPSGPTSNRTPSFGFTSSGVTLTCSIDQGTPAWTPC
                     TSPYAPPESLADGDWTFRVHAVSSLGSEADQTRTFTVDGQPPETILDGTPNQFTNEST
                     AYFDFHSTEPGSYFECDGGDDDWLLCSSPATIVQVSPGVSTFRVRATDSAGNVDPTPA
                     TFEFEMDQIKPETTIDSGPSGTITTSQADFAFSSNKSPAQFFCSIDGADAEACASTKT
                     YTGLSDGQHEFRVYARDKFGNQDSTPDSRTFTVDTSGPVDPPSNAFTFGRLKLNTKQG
                     TATLQVKVPGAGKVQLLGSKTVAASSKSARAKSTVTLAVKARGKATKTLKKRGKVGLA
                     VKVRFTPTGGTAKTMTKTVKLVRKKTGK"
     gene            1376..2308
                     /locus_tag="PLV77_01340"
     CDS             1376..2308
                     /locus_tag="PLV77_01340"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC14522.1"
                     /translation="MICGIAFLLSGAIGEAGFIPKLDRVKFDDGEVYRQGCIVGDGQL
                     RSRPCRFGYRNSKRRVVLFGDSHAAEWGPALLRLARADHWQVIVLTRASCPAALTNID
                     RYCNTWRHNSLKRIRRLRPGLVVVASAANYHHYWTLRKRRGLDRQASERPLVHGMIRT
                     LRYLKRWSRKVVLIRDHSAAPFSVTDCLRRNPTRPQRCDFRSTRRKAWSYEFKAAGRV
                     DGVGVIDPQPMLCPGRRCYAVDGRHLVYRNYGHLAAAWVRTRNRWLGRNLNDPWNGRD
                     VKPVLSNDSSDERSRPGYLVRSIPSPMNGQATSD"
     gene            complement(2343..2654)
                     /locus_tag="PLV77_01345"
     CDS             complement(2343..2654)
                     /locus_tag="PLV77_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14523.1"
                     /translation="MGVAACAVLLIAGCGGVAELDSSETDTVNAANSAVADATASGSL
                     KPGDQAKLEALMVLCHEKPLAESDGESMRELITRLAPELKSADPKMSRRLKRFATDGC
                     N"
     gene            2782..2997
                     /locus_tag="PLV77_01350"
     CDS             2782..2997
                     /locus_tag="PLV77_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14524.1"
                     /translation="MGVQTSKTPHTVRLEEASRRAHEAEANGDDAAAIAAWREYRLVR
                     DAGRPAEDLLEEGITLSKMADRLIEPR"
     gene            2994..3470
                     /locus_tag="PLV77_01355"
     CDS             2994..3470
                     /locus_tag="PLV77_01355"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNC14525.1"
                     /translation="MTDFGRILKDLNAAGVRYVVVGGIALIRHGVVRATRDVDVVYDP
                     DPSNLEAIRSLAEKWGATRPDGSPLLENAFSAGRSVHLSTPLGEIDLLSEETASVSFV
                     NLLERSEIRRIDAVEAPICSLADLVALKRAAGRERDLADLSDLEATHGSLPARPED"
     gene            complement(3559..3647)
                     /locus_tag="PLV77_01360"
     tRNA            complement(3559..3647)
                     /locus_tag="PLV77_01360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3611..3613),aa:Ser,seq:tga)
     gene            complement(3713..3892)
                     /locus_tag="PLV77_01365"
     CDS             complement(3713..3892)
                     /locus_tag="PLV77_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14526.1"
                     /translation="MSKMGEVESEEEIRKEMEELGVSNDATGSPDVLMSGPMIQVTVI
                     VLVVLLVAGALFLVF"
     gene            3931..>4323
                     /locus_tag="PLV77_01370"
     CDS             3931..>4323
                     /locus_tag="PLV77_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC14527.1"
                     /translation="MRRLLFLSCAVVFLDVTFFSVLTPLLPSYKSDHHLSEGAIGVLA
                     GAFAAGSLVMAVPGGWLVARVGARKTVIAGLAGIGICSPLFGFAENIVLLDLLRFLQG
                     GCGALMWAGAISWVVVAAPTARRGELLGI"
BASE COUNT          765 a         1410 c         1359 g          789 t
ORIGIN      
        1 tcatcaccgg cgccgcgaca actgacggaa tcgcggttga ttccagccac atctactgga
       61 ccttgccggg tccgggccgg atcgcccgcg ccaatctgga cgggtccggt gccaacacca
      121 gcttcatcca gaacgccggc aaccccgtcg ccgtcgcggt tgacagcggg gtaagcccgc
      181 caaccctgac gatcaattcc ggtccctcgg ggccgaccag caaccgcacg ccttccttcg
      241 gattcaccag ctccggcgtg actctcacct gctcgattga tcagggcact cctgcgtgga
      301 ctccctgcac ctcgccttat gccccgcccg agtccctggc cgacggtgac tggaccttcc
      361 gggtccacgc ggtcagctcg ctgggcagtg aggcggacca gacccgcacg ttcacggttg
      421 acggacagcc gccggaaacg atcctcgacg gtaccccgaa ccagttcacc aacgaatcca
      481 ccgcctactt cgacttccat tcgaccgaac ccggttccta cttcgagtgc gacggcggcg
      541 atgacgactg gcttctctgc agctctccgg ccacgatcgt ccaggtcagc ccgggagtca
      601 gtacgttcag ggtccgcgcg accgacagcg ccggcaacgt cgacccgacc ccggcgactt
      661 tcgagttcga gatggatcag atcaagccgg aaacgaccat cgactcaggt ccttccggaa
      721 cgatcaccac aagccaggcg gacttcgcct tcagctcgaa caagtcgccg gcccagttct
      781 tctgcagcat cgacggtgct gatgcggaag cctgcgcctc cacgaagaca tacacaggct
      841 tgtctgacgg ccagcacgag ttcagggttt acgcccggga caagttcggc aaccaggact
      901 cgaccccgga ctcccggacc ttcacggtcg acacctccgg tccggtcgat ccgccttcga
      961 acgccttcac cttcggcagg ttgaagctga acactaagca gggcaccgcg accctccagg
     1021 tcaaggtgcc gggcgccggc aaggtgcagc tgctcggctc gaagactgtt gccgccagtt
     1081 cgaagtcggc ccgggcgaaa tcgaccgtca ccctggccgt caaggccagg ggcaaggcga
     1141 cgaagaccct caagaagagg ggcaaggtcg gcctcgcggt caaggtccgg ttcaccccga
     1201 ccggtggcac cgcgaagacc atgacgaaga ccgtgaagct ggtcaggaag aagaccggaa
     1261 agtagcgccg gtgacatccc cgggcatgag tacttcttga cttggagagt caggattatg
     1321 cttgaatggg aagcggtttt tcatggttgg gcacggtcga atttctctgg ctgcggtgat
     1381 ctgcggcatc gcgtttctgt tgtcaggcgc cattggcgag gcgggcttca tcccgaagct
     1441 cgaccgcgtc aaatttgacg acggcgaggt ctatcgtcag ggctgtatcg tcggcgacgg
     1501 gcagctcagg tccaggccct gccggttcgg ctaccggaac tcgaaacgga gggtggtcct
     1561 gtttggtgat tcgcacgccg ccgagtgggg tcctgccctg ctcaggctgg cccgggccga
     1621 ccactggcag gtgatcgtcc tgacccgggc cagctgcccg gccgccctga ccaacatcga
     1681 ccgctactgc aacacctggc ggcacaactc cctgaagcgg atcaggcgtt tgcgacccgg
     1741 tctggtcgtg gtcgcctcgg ccgcgaatta ccaccattac tggacccttc gcaagcgccg
     1801 tgggctggac cgccaggcga gcgaacgccc gctggtccac ggcatgatca ggaccttgag
     1861 gtacctgaag cgctggtcga gaaaggtcgt tctgatccgg gaccactcgg ccgcgccgtt
     1921 cagcgtcacc gactgcctgc gcaggaaccc gacccgcccg cagaggtgtg atttcaggtc
     1981 aacccgcagg aaggcctggt catacgaatt caaggcggcc ggaagggtgg acggtgtcgg
     2041 agtgatcgat ccgcagccga tgctatgccc cggccgccgg tgctacgcgg tcgacggccg
     2101 ccacctggtc taccgcaatt acggccacct ggcagcggcc tgggtccgga cccggaaccg
     2161 gtggctcggc cggaacctga acgatccctg gaacggaagg gacgtcaagc cggtcctttc
     2221 gaacgattcg tccgatgaac ggtcacggcc cgggtacctg gtccggtcga tcccctcccc
     2281 gatgaacggc caggcaacca gcgattgagc gacccggttc cggctggccg gccggggagc
     2341 gctcagttgc agccgtcggt ggcgaaccgc ttgaggcgcc gggacatctt cggatcggca
     2401 ctcttcagct ccggtgcgag ccgggtgatc agctcgcgca tcgactcgcc atccgattcc
     2461 gccaggggtt tctcgtggca gagaaccatc aacgcttcga gcttcgcctg atccccgggt
     2521 ttcagcgatc cgctcgcggt ggcatccgcc acggccgagt tggcggcgtt caccgtgtca
     2581 gtctcgctgg agtcgagctc cgcaactcct ccgcaaccgg cgatcaacag caccgcgcat
     2641 gcggcaactc ccaaagctct cctcacatca acctccggac gggatcaagg acgggcgaag
     2701 cctacattcg caagccgtcc agcgggactc ttgccggccg aaacccacat gaaaccgacg
     2761 ctgtcgggct atccttgacc cgtgggcgtt cagaccagca agactcctca caccgttcgc
     2821 ctggaagagg cgtctagaag agcgcatgag gcggaggcaa acggcgatga cgcggctgct
     2881 atcgcggcct ggcgcgagta tcgcctcgtt cgtgacgctg gccgtcccgc cgaggatttg
     2941 cttgaggagg gaatcactct gagcaagatg gccgatcggc tcatcgagcc gcggtgaccg
     3001 actttggtcg catactcaag gacttgaatg ctgcaggggt ccggtacgtg gtagttggcg
     3061 gtatcgcgtt gatccgtcac ggtgtggtgc gcgccacccg ggatgtggat gttgtctatg
     3121 accctgaccc gtccaatctg gaggcgattc gttcgttggc agagaaatgg ggtgcgaccc
     3181 gacccgatgg gtcacctctc ctggagaacg ccttctccgc tggccggtcg gttcatctgt
     3241 cgacgccact gggcgaaatc gacttgcttt cggaagagac ggcctcggtg agctttgtca
     3301 acctgctgga gagatccgag atccgccgca tcgacgctgt cgaagccccg atctgttcac
     3361 ttgccgacct ggttgccctg aagcgcgcag caggcagaga gcgcgatctc gcggatctct
     3421 cggatctcga agcaactcat gggagcttgc cagcccggcc cgaggactga agcacgcccg
     3481 agcttcggct tcgagattgg cagaatcaca gaagcatcct gccaggcccg caccaagagc
     3541 gagatttgtg gtgtggagcg gagggggagg gattcgaacc ctcgatacgc cgtaagacgc
     3601 atgctggttt tcaagaccag tgcattcaac cgctctgcca cccctccgtg gcctgttcgg
     3661 cccggggatt ctgacaggcc gcgggtcgat tggcgggatt gagccgccgc ggctagaaga
     3721 cgaggaagag cgcgccggcg acgaggagga cgacgaggac gatcaccgtc acctggatca
     3781 tcggtcccga catcaggaca tccggggagc cggtcgcgtc attgctgact ccgagctcct
     3841 ccatctcttt gcggatctct tcctcggatt cgacttcacc catcttgctc atgccgtaac
     3901 tatcccacga ccgcctgtca ggatggggtc gtgcgtcgtc tcctctttct ttcctgtgcg
     3961 gtcgtcttcc ttgacgtcac attcttctcg gtactgaccc cgctgcttcc cagctacaag
     4021 agcgaccatc acctcagcga aggggcgatc ggggtgctgg ccggtgcatt tgccgccggt
     4081 tcgctggtca tggcggttcc gggcggctgg ctggtggcca gggtgggagc gaggaagacc
     4141 gtgatcgccg gcctggccgg gatcggaatc tgcagtccgc tcttcgggtt cgccgagaac
     4201 atcgtcctgc ttgatctgct ccgtttcctc cagggcggct gcggcgcgtt gatgtgggcg
     4261 ggcgcgatca gctgggtcgt cgttgcggcc ccgaccgcgc gccggggtga gctgctcggg
     4321 atc
//
LOCUS       DAOQMF010000048         4307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_9934_length_4307_cov_3.649341, whole genome shotgun sequence.
ACCESSION   DAOQMF010000048 DAOQMF010000000
VERSION     DAOQMF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9934_length_4307_cov_3.649341"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1295)
                     /locus_tag="PLV77_01375"
     CDS             complement(60..1295)
                     /locus_tag="PLV77_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HNC14528.1"
                     /translation="MAPGFGRAITSLPVRLLCGAFGLFLLGVAIYSGFKGTEAPDRNV
                     TLTLLFVTAWLGFPLLGVLVGNLFPAFNPWNAVAAPAGWAWRRVAGKAPAHLAYPERL
                     GRWPAVIGLFLFVWMELIYGEGSGVAVGVSPEAVAQAAVFYSIYTLVMTGLFGREAWF
                     RNGEIFSVYFGMFGSLGKFEVRGRELGIRRFLSGATTWPAGIAGSVALVITSIGTTTF
                     DGASEGVFKDGIQWLIDRFADIGFNPTAATRTSMTVFMLLSVTIVALVYLGGVRGMST
                     VPGAPDRPTLWRSFGHVLIPIAFAYLLAHYFSLFFFQEQAQFTYLLSDPLGTGTTDLF
                     GTASYGVNYNAISNQVIWYVQVAALVCGHVAGLVLAHDKAITIWKDYRTAARSQYWML
                     AVMVAFTCFGLFLLSVSNS"
     gene            complement(1451..2080)
                     /locus_tag="PLV77_01380"
     CDS             complement(1451..2080)
                     /locus_tag="PLV77_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNC14529.1"
                     /translation="MNTNLWDRIEQARAEHNVLEHPFYQRWSAGELTREELADYSGQY
                     RYATEAIAKLSTDIAAQAPESERAGLEAHAAEEHDHVVMWDGFVGAVGGTVGAEPNPE
                     TLACVEAWTKADGFGRQLARMFAIESGQPAISKTKTEGLGEHYEITDSPGNLYFTVHE
                     EMDVHHAAEGRKLIERYMNDFSEDELVEAAEEAFKANWLLLDGVEKARA"
     gene            2113..2964
                     /locus_tag="PLV77_01385"
     CDS             2113..2964
                     /locus_tag="PLV77_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14530.1"
                     /translation="MTEGGSYPYDHAILPLRDDIAAPELPGKATWIGKDPGKMPVLTA
                     KGPVLVHFLDYSQLNSVRTLPYLVEWHERYEPLGLRVLGVQAPRFPFGADPEAAGRGL
                     RQLGVEFPVLIDESKRLWTGYGCEGWPSLFLWGRGGILRWFHFGEGDYQGTEQAIQEA
                     LAASFERLPEMPAIMEPVRPTDAAGVEVSPPSPEVIPFPDRPLTIDDFDALALDFEGG
                     GAYVTADGKGDLRVTLDGEARGEVGIDGPGLYPLVETGRHGSHKLGIELEGAPEIWSV
                     SYSPAAT"
     gene            complement(2971..3942)
                     /locus_tag="PLV77_01390"
     CDS             complement(2971..3942)
                     /locus_tag="PLV77_01390"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNC14531.1"
                     /translation="MPETPSTGIEKVILAAPRGYCAGVDRAVQTVERALDNHGAPIYV
                     RKEIVHNKHVVEQLAARGAIFVEEETEVPEGEIVVFSAHGVSPAVHERAAARELRTID
                     ATCPLVTKVHVEARKFAAEGYTIIMVGHEGHEEVEGTMGEAPDSIVLVQTVDEVDHLE
                     IEDPDHVALITQTTLSVDETAGIIARLRERFPGIVTAKGEDICYATTNRQIAVKQLAR
                     QCDLVLVIGSTNSSNSNRLVEVAREHGSESFLIDNHTQVQEEWLDGVETVGITSGASA
                     PEELVEDLVQLFRDRGVEDVSELRTVDESVRFMLPKEIRKGLPMVNS"
     gene            3993..>4307
                     /locus_tag="PLV77_01395"
     CDS             3993..>4307
                     /locus_tag="PLV77_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNC14532.1"
                     /translation="MEPASHELDRLTAIDAQFLTNEHENAHMHIGAVMIFEGDPPTYD
                     EFKAHIGSRLDFVPRYRQKLVYPPYGTGRPLWADDPDFNLNYHLRHTALPRPGGEREL
                     GEL"
BASE COUNT          769 a         1368 c         1448 g          722 t
ORIGIN      
        1 tcccggcgtt gacgaagcca cggcgcccgc aggtgcgtcc tgggcgcgtt gcccgggcct
       61 taactgttcg agactgacag tagaaagagc ccgaaacagg tgaaggcgac catcacggcg
      121 agcatccagt actgggagcg ggcggcggtg cggtagtcct tccagatggt gatcgccttg
      181 tcgtgggcga ggacgaggcc ggcgacgtgg ccgcagacga gggcggcgac ctggacgtac
      241 cagatcacct gattggagat cgcgttgtag ttgaccccgt aggaagcggt tccgaagagg
      301 tcggtggtgc cggttccgag cggatcggag agcagatagg tgaactgggc ctgctcctgg
      361 aagaagaaga ggctgaagta gtgggcgagc aggtaggcga aggcgatcgg gatcaggaca
      421 tggccgaagg accgccagag ggtcggccgg tcgggggcgc cagggaccgt gctcatgccc
      481 cgcaccccgc ccaggtagac cagcgccacg atagtgacgc tgagaagcat gaagacggtc
      541 atcgaggtgc gggtcgcggc ggtcgggttg aaaccgatgt ccgcgaaccg gtcgatcagc
      601 cactggatcc cgtccttgaa gacgccttcc gaggcaccgt cgaaagtggt ggtgccgatc
      661 gaggtgatga ccagtgcgac cgagccggcg atcccggccg gccaggttgt cgctccggaa
      721 aggaagcgcc ggatgccgag ttcacggccc cggacctcga acttgccgag cgagccgaac
      781 atgccgaagt agaccgagaa gatctcgccg tttcggaacc aggcctcccg gccaaagagg
      841 ccggtcatga ccagggtgta gatcgagtag aagactgccg cctgggcgac cgcctcgggt
      901 gaaacgccga ccgccacccc gcttccctca ccgtagatca actccatcca gacgaagagg
      961 aacagcccga tcacggccgg ccagcgtccg agccgctccg gataggcaag gtgggcagga
     1021 gccttgcccg cgaccctgcg ccaggcccag ccggccgggg cggcgaccgc gttccatggg
     1081 ttgaaggcgg gaaagaggtt cccgaccagc actcccagca acggaaagcc gagccaggcg
     1141 gtgacgaaaa gcagggtcag ggtgacattt cggtccggcg cctcggtgcc tttgaagccg
     1201 gaatagatcg cgaccccgag caggaagagg ccgaaggccc cgcagaggag ccggaccggc
     1261 aggctggtga tcgcgcggcc gaaaccgggc gccacctgac gccaggaatc actctcgtag
     1321 cgggcccggc gccagcccgc tgagagagcg aagaacgaga ctacgagaac aatcgaggct
     1381 ccccaggcga agagccacgc cggtattggc aggtcctgcc cggcggacag gacatgtgcg
     1441 aggggaaggc tcaggcccgc gccttctcga ctccgtcgag cagcagccag ttggccttga
     1501 aggcttcctc ggccgcctcg accagctcgt cctcactgaa atcgttcatg taccgctcga
     1561 tcagcttgcg accctcggcg gcgtgatgga cgtccatctc ctcgtgaacg gtgaagtaga
     1621 ggttgccggg agagtcggtg atctcatagt gctcgccgag accttcggtc ttggtctttg
     1681 aaatcgccgg ctggcccgac tcgatcgcga acatgcgcgc gagctggcgg ccgaaaccgt
     1741 cggccttggt ccaggcttcg acgcaggcga gggtctccgg gttcggctcg gcacctaccg
     1801 tgccgccgac cgcgcccacg aagccgtccc acatgacgac gtgatcgtgc tcttcggccg
     1861 catgcgcctc gagcccggcc cgctcggatt ccggtgcctg ggcggcgatg tcggtgctca
     1921 gcttggcgat cgcctcggtc gcgtagcggt actggcccga gtaatcggcg agttcctcgc
     1981 gggtcagctc gccggccgac cagcgctggt agaagggatg ttccaggacg ttgtgctcgg
     2041 cccgggcctg ctcgatgcgg tcccacagat tcgtgttcac gagtttctct cctcgtagag
     2101 ctcgtagagg gggtgactga aggcggatca tatccatacg atcacgccat cctccctctt
     2161 cgagatgaca tcgcggctcc cgaactgccc ggcaaggcga cctggatcgg caaggacccg
     2221 ggcaagatgc cggtgctcac ggcaaaaggc cctgtactgg tccactttct ggattacagc
     2281 cagctgaaca gcgtgcgcac cctgccctac ctggtcgagt ggcacgagcg ttacgagccg
     2341 ctcggcctgc gggtgctcgg tgtccaggct ccacgctttc ccttcggcgc cgacccggaa
     2401 gccgccgggc gcggactgcg gcaactcggg gtcgagttcc cggtcctgat cgatgaaagc
     2461 aagcgactct ggaccggtta cggatgtgaa ggctggccga gcctcttcct ctggggtcgc
     2521 gggggaatcc tccgctggtt ccacttcggc gagggcgact atcagggaac cgagcaggcg
     2581 attcaggaag cgctggccgc ctccttcgag aggctcccgg agatgccggc gatcatggaa
     2641 ccggtccggc ccaccgacgc agcgggcgtt gaggtgtcgc caccctcacc cgaagtgatc
     2701 ccgtttccgg accgccccct cacgatcgat gacttcgacg cgctggcgct cgatttcgag
     2761 ggcggtggcg cctacgtcac ggccgatggc aagggagatc tgcgggttac cctcgacggc
     2821 gaagcccgcg gcgaagtcgg gatcgatggc ccggggcttt acccgctcgt cgaaaccggc
     2881 cgccacggca gccacaagct ggggatcgaa ctggaaggcg ctccggagat ctggtcggtc
     2941 agttactcgc cggccgcgac ctgagaccgg tcaggaattg accatcggga ggcccttgcg
     3001 gatctccttc ggcagcatga agcggaccga ctcgtcaacg gtgcggagct cggagacgtc
     3061 ctccaccccg cggtcgcgga aaagctgaac cagatcctcg accagttcct ccggtgcgct
     3121 tgccccggaa gtgatcccga ccgtttccac cccgtcgagc cactcctcct gaacctgggt
     3181 gtggttgtcg atcaggaagg attcggagcc atgctcgcgc gcgacctcga ccagccggtt
     3241 cgagttcgag gagttggtcg agccgatcac caggaccagg tcacactggc gggccagctg
     3301 cttgaccgcg atctgacggt tggtggtggc gtaacagatg tcctcgccct tggcggtgac
     3361 gattccgggg aacctttcgc gcaggcgggc gatgatcccg gcggtctcat cgaccgaaag
     3421 ggtggtctgg gtgatcaggg cgacgtggtc cggatcctcg atttcaaggt ggtcgacctc
     3481 gtcaacggtc tggaccagga cgatcgagtc cggtgcctcg cccatcgtgc cttcaacctc
     3541 ttcgtggccc tcgtggccga ccatgatgat cgtgtagccc tcggccgcga acttgcgggc
     3601 ctcgacgtgg accttggtga ccagcgggca ggtcgcgtcg atcgtgcgca gctcccgcgc
     3661 cgcagcccgt tcgtgaaccg ccggcgaaac gccgtgggcg gagaagacga ctatctcgcc
     3721 ttcgggcacc tcggtttcct cttcgacgaa gatcgctccc cgcgccgcca gctgctcgac
     3781 cacgtgcttg ttgtggacga tttccttgcg gacgtagatc ggggcaccgt ggttgtcgag
     3841 cgcgcgctcg accgtctgca cggcccggtc gacgccggcg cagtagccac ggggcgcggc
     3901 cagaatgact ttctctatcc cggtggaggg tgtttcgggc attggacaag ggtacccgag
     3961 tagagtcttg tgctagtcca cgatggggag ccatggaacc cgccagccac gaacttgacc
     4021 gactcactgc gattgatgcg cagtttctga ccaacgagca cgaaaacgct cacatgcaca
     4081 tcggtgcggt gatgatcttc gaaggggatc cgccgaccta tgacgagttc aaggcccaca
     4141 tcggctcgcg gctcgatttc gtgcccaggt accgccagaa gctggtctat ccgccttacg
     4201 gcaccggccg tccgctctgg gccgatgatc ctgatttcaa cctgaactac cacctgcgcc
     4261 acaccgccct gccgaggccg ggtggcgaac gcgagctggg cgagttg
//
LOCUS       DAOQMF010000049         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10091_length_4268_cov_3.960361, whole genome shotgun sequence.
ACCESSION   DAOQMF010000049 DAOQMF010000000
VERSION     DAOQMF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10091_length_4268_cov_3.960361"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PLV77_01400"
     CDS             <1..603
                     /locus_tag="PLV77_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNC14533.1"
                     /translation="KTWDPMVDYAGMSRNVRAFMSLFHCTKPVICKVHGFCVAGGTDL
                     ALCSDLLVIADDAKIGYPPARVWGSPTTSLWAYRLGAQRAKRLLFTGDSLTGREAAEA
                     GLAIESHPREKLDEAVDALAARIAMVPLNQLQMMKLLVNQSLYAQGLHQTQLIGTVFD
                     GITRHTPEGFAFQQRAAEAGFKQAVRERDEPFGDFGLKPD"
     gene            complement(620..985)
                     /locus_tag="PLV77_01405"
     CDS             complement(620..985)
                     /locus_tag="PLV77_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HNC14534.1"
                     /translation="MPTSEENDKARTNFAAARTTLAGERTQLAWVRTGLASIAVGIGL
                     ARVVPLLDSGAIAWPYEALGVAYCAFGLGLIFFGVQRGKLSAEHHVEKPTVPAGGAFL
                     AAGGLILGIATILVVALAR"
     gene            1031..1429
                     /locus_tag="PLV77_01410"
     CDS             1031..1429
                     /locus_tag="PLV77_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNC14535.1"
                     /translation="MGERWRIGSGAVWEERFGYSRAVKAGPLIFVSGTTAADPTGGPP
                     IGGDDIAAQARECFRRIEVALGELGSGLGEVVRTRMFVTDIGLSEAVGLVHGEIFGRQ
                     APAATMVEVSALISPDLLVEIEVDAVSSSD"
     gene            1554..2159
                     /locus_tag="PLV77_01415"
     CDS             1554..2159
                     /locus_tag="PLV77_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14536.1"
                     /translation="MSNLVTRIKQFRPATLIAMLALFVAIGGTATAASGLINGKKIKN
                     NTVTGKKLKNRTITKNKLAPATVKALKGQQGPKGDTGQPGKDGVVSPTYQEFGSTNIV
                     DGNELAIGTVNVPAGKYMVTATANTFALGSGRTECFVTTNNGGGSSETSSWNSTAANV
                     RNALPVVYVTDTDTVTSITLGCGIDDTNGSVGGSMIVTPVQ"
     gene            complement(2252..3250)
                     /locus_tag="PLV77_01420"
     CDS             complement(2252..3250)
                     /locus_tag="PLV77_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC14537.1"
                     /translation="MTTGSIDVHSHYIAPAYRSALESVGKDRIGGIPVPPWTPELAIG
                     FMDAHGIARQVLSVSDPGVDFLAGEAAAKLARECNDYLAGVVASHPDRFSGLAVVSGN
                     DPEAALSEIARCLERPGFAGLGLLSSYDGSYLGDPAFDPLLTELDQRHAWVMVHPTAV
                     PTTDKPDLTIPDFMAEYPFDTTRAFLNLLVHNAFARFPGIRWHFAHGGGTIPMLAARM
                     DVAAAHGKVLGPLLGLPEQSADLGPESAREALAGCWYDTALIASEGGLQAVSAMTGTD
                     HILFGCDWPFAGLAYPPEGDPQPAIGTLFGPDDQEAIYRANAEKLLAGLPQQTESA"
     gene            complement(3251..3919)
                     /locus_tag="PLV77_01425"
     CDS             complement(3251..3919)
                     /locus_tag="PLV77_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC14538.1"
                     /translation="MTVHTSSIREKNDTKPPGRVERRKAKTRASLIEAARRLFASKNI
                     ESTSVAEIAEEADIAVGSFYNYFETKDELLAALLESTLVEQLARIRARQSQTDDPAEK
                     VAIGHRHLVDLATREPDLAWLIVRFEVPQRIASLGLAEAARADMMEGIATGRFDVRNP
                     ELSLRASGGALLGVIHTILLGELPGDSGGDLAAGVLMGLGMKPAEAREVAARPLPPES
                     LEAE"
     gene            3981..>4268
                     /locus_tag="PLV77_01430"
     CDS             3981..>4268
                     /locus_tag="PLV77_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC14539.1"
                     /translation="MNRVPVLVVGGGGAGLTASMLLANLDIEHLLINSRPGTSDLPKA
                     HVLNLRAMEILDDVGAADRISEKSTPPEAMAATAFYAGFGGDDPDAGRRIAK"
BASE COUNT          790 a         1361 c         1416 g          701 t
ORIGIN      
        1 aagacctggg atccaatggt cgactacgcc ggcatgagcc ggaacgtgcg ggccttcatg
       61 tcgctgtttc actgcaccaa accggtgatc tgcaaggttc acggcttctg cgtcgccggt
      121 ggcaccgacc tggccctgtg cagcgacctt ctggtgatcg ccgacgacgc caagatcggc
      181 tacccgccgg cccgggtctg gggttctccg accacttcgc tctgggctta caggctcggg
      241 gcgcaacggg ccaagcggct gctcttcacc ggtgacagcc ttaccggccg ggaagcggca
      301 gaagccggtc tggcaatcga gtcgcacccg cgtgagaagc tcgacgaggc agtcgacgcg
      361 ctggccgccc ggatcgcaat ggttcccctg aaccagctgc agatgatgaa gctcctcgtc
      421 aaccagtcgc tctacgccca gggcctccac cagacccagt tgatcggcac ggtctttgac
      481 ggcatcaccc gccacacccc ggagggcttt gccttccagc aaagggcggc cgaagccggc
      541 ttcaaacagg cggttcgcga acgcgacgaa ccattcgggg acttcggcct gaaaccggac
      601 tgagccgggc gggtccggct cagcgggcga gcgcgaccac gaggatcgtt gcgatcccga
      661 ggatgagccc tcccgcggcg aggaacgctc cgccagccgg cacggtcggc ttctcgacat
      721 ggtgttcggc cgagagcttg ccgcgctgga cgccgaagaa gatcaggccc aggccgaagg
      781 cgcagtaggc aacgcccagg gcttcatagg gccaggcgat cgctccggaa tcgagtagcg
      841 ggacgactct cgccaatccg atgccgaccg cgatcgaggc gagtcccgtc cgcacccatg
      901 ccagctgggt tcgctcaccg gccagcgtcg tgcgtgcggc ggcgaagttt gtccgggcct
      961 tgtcgttctc ttcggaggtg ggcatgacgc aatggtaatc cggccaccgc gcggatacgc
     1021 tgaaccggac atgggggaga gatggagaat cggctccggc gccgtctggg aagaaagatt
     1081 cggctattcg cgggccgtca aggccgggcc cctgatcttc gtctcgggaa ccaccgcggc
     1141 ggatccgacg ggaggacccc cgatcggagg cgatgacatc gccgcccagg cccgggagtg
     1201 ctttcgccgg atcgaagtcg cgctcgggga gctcggctcg ggcctcggcg aagtcgttcg
     1261 aacccgcatg tttgtgactg acatcggcct gagcgaagcg gtcgggctgg tccacggaga
     1321 gatcttcggc aggcaggcac cggcggcgac catggtcgag gtctcggccc tgatcagccc
     1381 cgatctgctc gtggagatcg aggttgacgc agtttcttcc tcggactgaa ccgctctgct
     1441 aagcgaaata tcggggttcc ggaaaagatt ttcgaaatcc gcgtaagtaa ttggctgctc
     1501 gccagttgac caatcagaag cgccaatcaa ccgaagcaca acgaaaggca acaatgagca
     1561 acctcgtcac ccgcatcaag cagtttcgcc cggccaccct gatcgccatg ctggccctct
     1621 tcgtcgccat cggcggcacc gcaaccgcgg cttccggcct gatcaacggc aagaagatca
     1681 agaacaacac ggtcaccggc aagaaactga agaacaggac gatcaccaag aacaagctcg
     1741 ctccggccac ggtcaaggcc ctcaaggggc agcagggccc gaagggcgat accggccagc
     1801 ccggcaagga cggcgtcgtc agcccgacct accaggagtt cggcagcacc aacatcgtcg
     1861 acggaaacga actcgcgatc gggaccgtca acgtcccggc cggcaagtac atggtcacgg
     1921 caactgcgaa taccttcgcc ctcggcagcg gccggaccga atgcttcgtc acgaccaaca
     1981 acggcggcgg cagcagcgaa acctcgagct ggaactcgac tgccgcgaac gtccgcaacg
     2041 ccctgccggt cgtttacgtc accgacaccg acacggtcac ctcgatcacc cttggctgcg
     2101 gcatcgatga caccaacggc tcggtcggcg gttcgatgat cgtcaccccg gtccagtaac
     2161 gaaccttcgg ctggaagcca tagagctaca cgcgccgccc ccgggaacgg gggcggcgtt
     2221 cgtgcatcgt cgtcgccctg gccctcgcgc cctaggctga ttcggtctgc tgcggaaggc
     2281 cggccagcag cttttccgca ttggcccggt agatcgcctc ttgatcgtcc gggccgaaca
     2341 gggtgccgat cgccggctgg gggtcaccct cgggcggata ggcgagaccg gcaaagggcc
     2401 agtcgcagcc gaagagaatg tgatcggtgc cggtcatcgc gctaaccgcc tgaagtccgc
     2461 cttccgaagc gatcaacgcc gtgtcatacc agcatccggc gagcgcctcc cgggcgctct
     2521 cgggaccgag atcggccgac tgctccggta gcccgaggag cgggccgagc accttgccat
     2581 gggcggcggc cacgtccatg cgggcggcga gcatcgggat ggtgcctccg ccatgggcaa
     2641 agtgccagcg aatgccggga aagcgggcaa acgcgttgtg gaccagaagg ttgaggaagg
     2701 cccgggtggt gtcgaacggg tactcggcca tgaagtcggg gatggtcagg tcgggtttgt
     2761 ccgtggtggg gacagcggtc gggtggacca tgacccaggc atgccgctgg tccagttcgg
     2821 tcaggagcgg gtcaaaggcg ggatcaccga gatagctgcc gtcgtagctc gagagcagcc
     2881 cgaggcctgc aaatccgggt ctctcgaggc accgggcgat ttcggaaagc gccgcttccg
     2941 gatcatttcc ggatacgact gccaggccgc tgaagcgatc gggatgggag gcgaccacgc
     3001 cggcgaggta gtcgttgcat tcgcgggcca gcttcgccgc cgcctcgccg gcgaggaagt
     3061 cgacgccggg atcggagacc gaaagcacct gtcgcgcgat tccgtgggcg tccatgaagc
     3121 cgatcgcaag ttcgggggtc cagggtggca ccggaatgcc gccgatccgg tccttgccga
     3181 ccgactcgag cgccgaccgg tacgcgggag cgatgtagtg cgaatgaacg tcgatcgatc
     3241 cggttgtcat ctattctgcc tccaggcttt cggggggaag gggccgggct gcgacctccc
     3301 ttgcctcggc cggtttcatg ccgaggccca tcagtacgcc ggcagcgaga tccccgccgg
     3361 agtcgccggg caactctccg agcaggatgg tgtggatcac gccaagcagg gctccgccgg
     3421 aggcgcgaag cgacagctcc gggttcctga cgtcaaatcg gccggttgcg atgccttcca
     3481 tcatgtccgc tcgggcggcc tcggcgaggc cgagactggc aattcgctgg ggtacctcga
     3541 agcgcacgat cagccaggca aggtcgggct ccctggtcgc caggtcaacc aggtgacggt
     3601 gcccgatcgc aactttctcg gccggatcgt cggtctgcga ctgccgggcc cggatccggg
     3661 cgagttgctc gaccagagtg ctttcgagca gagcggcgag cagctcgtcc ttggtctcga
     3721 agtagttgta gaaggagccg acggcgatgt ccgcttcttc ggctatctcg gcgacgctgg
     3781 tcgactcgat gttcttcgag gcgaacagtc gcctggcggc ctcaatcagg gaagcacgtg
     3841 tcttcgcctt gcgtcgctcg acccgccctg ggggtttggt gtcgttcttt tcccggatcg
     3901 atgaagtatg gacggtcatg ataacatgag tatagcgtca aacttgaata agtgttcatg
     3961 tttgtgctac ggtcgtcgac atgaaccgag tacccgtact ggtcgtggga ggcggcgggg
     4021 ccggattgac ggcctcgatg ctccttgcga acctcgacat agagcatctg ctgatcaact
     4081 cccgtcccgg gacttcggac ctgccaaaag cgcatgtcct caacctgagg gcgatggaga
     4141 ttctcgatga cgtcggcgcc gccgaccgaa tcagcgagaa gagcacgccg ccggaggcaa
     4201 tggcggcgac cgccttctac gccggcttcg gcggggacga cccggacgcc ggacgccgca
     4261 tcgcgaag
//
LOCUS       DAOQMF010000050         4238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10213_length_4238_cov_3.878795, whole genome shotgun sequence.
ACCESSION   DAOQMF010000050 DAOQMF010000000
VERSION     DAOQMF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10213_length_4238_cov_3.878795"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2156
                     /locus_tag="PLV77_01435"
     CDS             <1..2156
                     /locus_tag="PLV77_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC14540.1"
                     /translation="GVPIDVNVAFPDHTQFGDGPYPLVYVFHGYGGGKINFTGMQHWL
                     DKGYAVFSQTNRGFHESCGTATSKADDADCVTKGFVRLDDTRYEVRDAQLFAGMLVDE
                     DLVQPNKIAATGGSYGGGMSMALAALKDRIMLPDGTYAAWTSPQGTPMSIAVAIPDIP
                     WTDLAYSLVPNGSNIDYIKDASYYGPFGIMKQSYVNGLYISGLGAPGYYTDPGTQPEA
                     DLAGWKAFMDAGEPFEGEPAAEQMLTEVTTHHSSYYIDHSQAPAPLLISSGFTDDLFP
                     ANEATRFYNRTRAQYPNSPIGLFFGSFGHMRGQNGSETVNDRAALQDAWVDYYLKGTG
                     SQPASNVTTWTQVCPNGATDGGPYVTDNWVDQSPGEIVLTDTGSKTIEPTGGDRAAGG
                     TFNPAPSGTACATASGTPETGIANYETAAAPAGGFTVMGSPTVIARYSYSSDEPPQVA
                     ARLVDISADGTTKTLVNRGVWRLPDTGFQVFQLNPNGWHVDEGHKLRLELLPFDGGNA
                     DPVAGLLSNFGRPTNNEPAITVEDLELRVPVKEQPGSLSGLVKAPAKRVLPNRPGVEL
                     APGNEDIGSETIEQYRERNYPVVGKIKITGATVKGKTLTAKVFCAGSNDSCARAKVSF
                     KGAPKKGKGKGVVLATKSNVTGPAGKTTAVRFNLTAKARALFKDTVKRKVTRKNGRKK
                     VKKVKVSGLKSLRSQVLIGGKSAGFITVKRTGKVN"
     gene            2432..>4238
                     /locus_tag="PLV77_01440"
     CDS             2432..>4238
                     /locus_tag="PLV77_01440"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC14541.1"
                     /translation="MRKFAVAFAALFMLVVVPNAFAAPPSSIFNGTIPCTTVGDGRTV
                     CQGPGGDTNATPGVNNAVANTVPSWDGTPIDVTVVLPDASTFGPGPYPLAMYFHGFGG
                     GKEGADGDLKRFTDLGFAAFSMTERGFKFSCGKPNAIIALNNDTPDACDKGFIHLMDT
                     RYEVRDAQYFAGLLADEDLIQPKKIGTAGASYGGAKSISLGALRNRVMLPDGSLIPWQ
                     SPEGKDMEIAVAAPIVPPSDFAYSLVPNGRTYDYTRFNTYLGPGGDAPFGVMKSAITN
                     GLASAGDSFSGEHGTDLDPLWDINGWKVMMNAGEPVDTAVATTMTDEMTTHHSAYYID
                     HSIEPSPMIIAQGLTDDLFPISESIRFYNRTKSEYPNSTVGLLFADIGHPRAPLEEPN
                     EQGRPADMELGYTTVEKWFDYYLNGTLPKPDDGVTAMSMVCPYSEPSGGPYTSDTWAD
                     FAPGEMRLSDPAKRVIAADGGDDNVASNFMTLIPACTQQQETEEPGIAQYDFPVSSGA
                     GYTLAGSATIIADVSVANGGESQIAGRLLEITPDGNQRLIARGVYRPESSGTQVIQLN
                     ANVYHFAPGTKMRIQLLPRDGSTGLPQLSSFRPSND"
BASE COUNT          816 a         1480 c         1284 g          658 t
ORIGIN      
        1 acggggttcc gatcgacgtc aacgtcgcct tccccgacca cacccagttt ggcgacggcc
       61 cctacccgct ggtctacgtc ttccacggct acggtggcgg caagatcaac ttcacgggca
      121 tgcagcactg gctcgacaag ggctacgcag tcttctccca gaccaaccgc ggcttccatg
      181 agtcctgcgg taccgccacc tcgaaggccg atgacgccga ttgcgtgacc aagggcttcg
      241 tgcgtctcga cgacacccgt tacgaagtcc gcgacgcgca gctcttcgcc ggcatgctgg
      301 tcgacgagga cctggtccag ccgaacaaga tcgccgcgac cggcggcagc tacggcggcg
      361 gcatgtcgat ggccctggcg gcgctgaagg accgcatcat gctcccggac ggcacctacg
      421 ccgcctggac cagcccgcag ggcactccga tgagcatcgc ggttgcgatt ccggacatcc
      481 cgtggaccga ccttgcctac tccctggttc ccaacggcag caacatcgac tacatcaagg
      541 acgcctccta ctacggcccg ttcggcatca tgaagcagtc ctacgtgaat ggcctctaca
      601 tcagcggcct tggcgccccc ggctactaca ccgatcccgg cacgcagccg gaagcggacc
      661 tggccggctg gaaggccttc atggatgcag gcgagccctt cgagggcgaa cccgccgccg
      721 aacagatgct gaccgaggtg accacccatc actcctcgta ctacatcgat cacagccagg
      781 ccccggcccc gctgctgatc agctcgggct tcacggacga cctgttcccg gccaacgagg
      841 cgacccgctt ctacaaccgg acccgggccc agtacccgaa ctcgccgatc ggcctgttct
      901 tcggctcctt cggccacatg agaggccaga acggctccga gacagtcaat gaccgggcgg
      961 ccctccagga cgcctgggtc gactactacc tgaagggaac cggctcccag ccggcctcga
     1021 acgtgaccac ctggacccag gtctgcccga acggggcgac cgatggcggc ccttacgtga
     1081 ccgacaactg ggtcgaccag tcgccgggcg agatcgtcct gaccgatacc ggcagcaaga
     1141 cgatcgaacc gaccggtggc gaccgggctg ccggcggcac gttcaacccg gcaccttccg
     1201 gcacggcctg tgccaccgcg tcgggaaccc cggagaccgg catcgccaac tatgagaccg
     1261 ccgccgctcc ggccggtggt ttcacggtga tgggctcgcc gaccgtgatc gccaggtaca
     1321 gctacagcag tgacgagccg ccgcaggttg cggcgcgcct ggtcgacatc tcggccgacg
     1381 gcaccaccaa gacactggtc aaccgcggag tctggcgcct gcccgacacc ggtttccagg
     1441 tcttccagct gaacccgaat ggctggcatg ttgacgaggg ccacaagctc cgtctcgagt
     1501 tgctgccctt cgacggcggc aacgccgatc cggtcgccgg actgctgtcc aacttcgggc
     1561 gtccgaccaa caacgagccg gcgatcaccg tcgaggatct cgaactcagg gtcccggtca
     1621 aggaacagcc cgggtcgctg agcggtctgg tcaaggcacc cgccaagcgg gtcctgccga
     1681 atcgtccggg tgttgaactc gcccccggca acgaggacat cggctcggag accatcgagc
     1741 agtaccgcga gcggaactac ccggtggtcg gcaagatcaa gatcaccggc gccaccgtca
     1801 agggcaagac cctgaccgcg aaggtgttct gcgccggctc gaacgactcc tgcgcccggg
     1861 ccaaggtgtc cttcaagggt gccccgaaga agggcaaggg caagggcgtc gtactggcga
     1921 ccaagagcaa cgtcaccggc ccggccggca agaccacggc tgtcaggttc aacctgaccg
     1981 caaaggcccg cgcgctgttc aaggacaccg tgaagcgcaa ggtgacccgc aagaacggcc
     2041 gcaagaaggt caagaaggtc aaggtcagcg gactcaagtc gctccgcagc caggtgctga
     2101 tcggcggcaa gtcggccggc ttcatcaccg tcaagcggac cggcaaggtc aactaaacga
     2161 gagtctccgg acgacaccgg gggtacctga aaagtaacga gcgcttcgcg ctctggttaa
     2221 ggagtcactt ttcaggcacc cccggtaccg tcctgaccca acgagatgct cagggagtcc
     2281 gtcgacggcg ggctcctttt tgtgttggtt tcgtgaacgg gtggtaatct caatagccag
     2341 gggatgtgcc aaccggcgcg tgcccgagag agtcgtccaa gggaagccgc agggagcggc
     2401 aaccgtttca atagggagaa acagggatca aatgcgtaag ttcgccgtag cgttcgctgc
     2461 acttttcatg ctcgtcgtcg tgcccaatgc cttcgccgcg ccgccgagct cgatcttcaa
     2521 tggaacgatc ccctgcacga ccgttggaga cggtcgaacc gtctgccagg gtccgggcgg
     2581 cgacaccaac gccacgcccg gtgtgaacaa tgcggtcgcc aacaccgtcc ccagctggga
     2641 cggcaccccg atcgacgtca ccgttgtcct gcccgacgcc agcaccttcg gccccggtcc
     2701 ctacccgctg gccatgtact tccacggttt cggcggcggc aaggagggtg ccgacggcga
     2761 cctcaagcgg ttcaccgatc tgggcttcgc cgccttctcg atgaccgagc gcggcttcaa
     2821 gttctcctgc ggcaagccga acgcgatcat cgccctgaac aacgacaccc ctgacgcctg
     2881 tgacaagggc ttcatccatc tgatggacac ccgctacgag gtcagggatg cgcagtactt
     2941 cgccggcctg ctcgccgacg aggatttgat ccagccgaag aagatcggca cggccggagc
     3001 ctcctacggc ggcgccaagt cgatttcgct gggcgccctg aggaaccggg tgatgctccc
     3061 cgatggttct ctcattccgt ggcaaagccc ggagggcaag gacatggaga tcgcggtcgc
     3121 cgccccgatc gtgcccccga gcgacttcgc ctactccctg gtgccgaacg gccggaccta
     3181 cgactacacc cgcttcaaca cctacctcgg ccctggcggc gacgcaccgt tcggagtcat
     3241 gaagtccgcg atcaccaacg gcctcgcctc ggccggcgac tccttcagcg gcgagcacgg
     3301 caccgacctc gatccgctct gggacatcaa cggctggaag gtcatgatga acgcgggcga
     3361 gccggtcgat acggcggtcg ccacgacgat gacggatgag atgaccaccc atcactccgc
     3421 ctactacatc gatcacagca tcgagccgtc accgatgatc atcgcccagg ggctgaccga
     3481 cgatctcttc ccgatcagcg agtcgatccg tttctacaac cggaccaaga gcgagtaccc
     3541 gaactccact gtcggtctgc tgttcgccga catcggccat ccccgggccc cgctggagga
     3601 gcccaacgag cagggacgcc cggctgacat ggagctgggt tacacgaccg tggagaagtg
     3661 gttcgactac tacctcaacg gcaccctccc gaaacccgat gacggggtta ccgcgatgag
     3721 catggtctgc ccgtacagcg agccttccgg cgggccttac acgtccgata cctgggccga
     3781 cttcgccccc ggtgagatgc ggctcagcga cccggccaag agggtgatcg ccgccgacgg
     3841 tggcgacgac aacgtggcca gcaacttcat gaccctgatc ccggcctgca cccagcagca
     3901 ggaaaccgag gaaccgggaa tcgcccagta cgacttccct gtttcgagcg gggcgggata
     3961 cacgctggcc ggttccgcga ccataatcgc cgacgtttcg gtggccaacg gtggcgagtc
     4021 gcagatcgcc ggccggctgc ttgagatcac cccggacggc aaccagcgac taatcgcccg
     4081 cggcgtctac cgaccggaga gctcgggcac ccaggtcatc cagctgaacg ccaacgtgta
     4141 ccacttcgct ccgggcacga agatgcggat ccagctgctg cctcgggacg gcagcaccgg
     4201 gttgccgcag ctctcctctt tccgcccgtc gaacgatc
//
LOCUS       DAOQMF010000051         4238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10214_length_4238_cov_2.849151, whole genome shotgun sequence.
ACCESSION   DAOQMF010000051 DAOQMF010000000
VERSION     DAOQMF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10214_length_4238_cov_2.849151"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLV77_01445"
     CDS             complement(<1..524)
                     /locus_tag="PLV77_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14542.1"
                     /translation="MAATLVAGGGAVLSGRAAADVWDIRKDRGNLEVKRRNSHRSGEF
                     VLDRRGVAEARPVRVRRSRYFNPAQVTRKYGIPILRPSWILLDLARELPDDEFGHAFK
                     EADRLGLLNEEELRACLELGSGLEGIRKFQNLVARRHPDLKDVRTLLETLFLELCARY
                     GIETPRVNRRKGRY"
     gene            complement(948..1508)
                     /gene="thpR"
                     /locus_tag="PLV77_01450"
     CDS             complement(948..1508)
                     /gene="thpR"
                     /locus_tag="PLV77_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNC14543.1"
                     /translation="MSGSSRQNRKRRVFVALDPPVELRSEAEAWARHIACSAKGLRVV
                     PARNSHITLAFLGERDEFEIEEVAGALLETAVPATGLALGAPAWLPKRRPRSLVLDIH
                     DDRGELKELQAGMAGALKSALGWKEDRPYRPHLTCARTGRGFDPSHLRVPVSPSIAFS
                     GQSVTLYASHLGPEGAEYEVIERVDQ"
     gene            complement(1519..2646)
                     /locus_tag="PLV77_01455"
     CDS             complement(1519..2646)
                     /locus_tag="PLV77_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNC14544.1"
                     /translation="MSTQSGPITVYGATGYTGKLVAAELASREVPFIVAGRNREKLEA
                     LADRLDGPGGKPESHAVATTDPRGLKELFARSGAVIACAGPFSLHGEPVLAAAVEAGT
                     HYLDTTGEQPFVRLAFDTYGPKALGKGSAVIPAMGFDYVPGDMLAALTAAALGAGETV
                     DKVRLGYHAPFQPSQGTMRSGIEMIKGGDFEWRERALRPASQAVGRPDFDFGGELGLQ
                     KMMHYPSAEHITVPTHLSPRSVETGLTVASFAPNAASQAVMPAFTRALGLAMRTPLRS
                     LMSKAISAMPEGADDQGRTASRFEIGCEVTSARRMARGRLTGTDVYGLTAALIVDGAI
                     RASRGEVRGSGALAPAAAFDPATFLSGLDRFGFNWEVTPPR"
     gene            2707..3600
                     /locus_tag="PLV77_01460"
     CDS             2707..3600
                     /locus_tag="PLV77_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNC14545.1"
                     /translation="MSFETILYSVEGPVATITLNRPGRLNTIVPPMPDEVEQAVGEAT
                     RDPEVKVIVLRGAGRSFCAGYDFGGGFEHWDEYLTTDGEWDPGKDFALATATELSPTQ
                     KFMSIWRSPKPVIAQVHGYCVGGGSDFALCADLIVASEDAVIGTPYSRMWGAYLSGMW
                     IYRLGLARTKYHALTGRPLNGKQAAEVELINQAVPFEELEATVAGLAADLASIPASQL
                     AAMKLVVNQAYENMGLASTQTLGPILDGLMRNTPEALEFIDKAESEGVGRVIEDRDGG
                     FGDYSQAPPELQPDPRHMIEP"
     gene            3600..>4238
                     /locus_tag="PLV77_01465"
     CDS             3600..>4238
                     /locus_tag="PLV77_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HNC14546.1"
                     /translation="MPASRARRNPDEHYGASNLELFFDLVFVFAITQVSHLLLENLTW
                     RGALEAAMVLLVVWWAWQYTTWATNELDTDRMPVRFVLLVIMMACLLMAVAIPEAFGD
                     KGLLFALSYVAIQIGRQAFLAFSAEPHTIARARGLHILIWFCFAAPFWIVGALVDGDA
                     RIAFWLVALTIDYCGPFVTYYVPWLKKVSPDAWKIGSGHFAERFQLFTIIALG"
BASE COUNT          688 a         1380 c         1445 g          725 t
ORIGIN      
        1 aagtatcggc ccttgcgacg gttcactctt ggagtctcga tgccgtatct ggcgcagagt
       61 tcgaggaata gggtctcgag aagagtccgg acatccttaa ggtccgggtg tcgccgagcg
      121 acgagattct ggaactttct gattccttcg agtccggaac ccaactcaag gcaagctctc
      181 aactcttctt cattgagcaa gcccaggcga tccgcttcct tgaaggcatg tccgaactca
      241 tcgtcgggga gttcccgggc tagatccagc aggatccagg aaggtcgcag gatcgggatt
      301 ccgtatttgc gggtgacctg agcgggattg aagtatcgac tcctgcgaac cctcaccggt
      361 ctcgcctcgg caacacctcg acgatccaga acgaactcgc cagatctgtg gctgttgcgc
      421 cgtttaacct caaggttccc gcggtctttt cgaatgtccc aaacatcggc agcagcccgg
      481 ccggagagga ctgcaccgcc cccggccaca agagtggcgg ccatgcaggc agccagtcga
      541 ccctcggcgc ggcggccaac cgcaaagacg gccctgcccg ccggcagaag gcgattctgg
      601 tcttgacgat gcttgatcat cgccttggaa aggctcgctc cgagcatctg cgttcgggtc
      661 acaagcccga attgcttccc cgcgatcctc tggagtgagg cttcgatttc cgctcggtca
      721 gacatccgtc gagcctgcca cgaaaaagct caaaagtgtg caaactgagc aacgattcac
      781 acacatgccg gtctcagatg ccggtctcag ccaaccgtcg ccgagacgga agcgtaggcc
      841 tgaccgatta ctccgggttg cgtttaggct gccggggtgc cccaaaggtc acccggaagg
      901 tgggtagggc gtcaggctgg ccggtccggc ccgggccggg tcggcttcta ctgatcgacc
      961 cgttcgatca cttcgtattc ggcgccctcg ggcccaagat gggaggcgta gagcgtcacc
     1021 gactgaccgc tgaaggcgat cgaggggctg accggcaccc gcagatgaga gggatcgaag
     1081 ccgcggccgg tcctggcgca ggtgaggtgc gggcggtagg gccggtcctc cttccagccc
     1141 agggccgact tcagggcccc ggccattccg gcctgaagct ccttcagttc gccccggtca
     1201 tcgtggatgt ccaggaccag gctgcggggc cgccgcttcg gcaaccaggc cggggcaccg
     1261 agcgccaggc cggtcgccgg gacggccgtc tcgagcaggg cacccgcgac ctcctcgatc
     1321 tcgaattcgt cacgctcgcc gagaaaggcg agggtgatgt gggaattgcg ggccggcacc
     1381 accctgaggc ccttggcgct gcaggcgatg tgacgggccc aggcctccgc ttcggagcgc
     1441 aactcgaccg gcgggtcaag ggccacgaag acccggcgct tgcggttctg tcgactgctc
     1501 ccgctcaccc gcctgaggtt agcggggcgg ggttacctcc cagttgaatc cgaagcggtc
     1561 gagcccggaa aggaaggtgg ccggatcgaa ggcggcggcc ggggccaggg cgccggagcc
     1621 cctgacctcg ccgcgggatg cccggatcgc gccgtcgacg atcagcgccg cggtgagccc
     1681 gtagacatcg gtcccggtca ggcggccgcg tgccatgcgc cgggcggaag tgacttcgca
     1741 gccgatctcg aaccggctgg cggtacggcc ctggtcgtct gcgccctccg gcatcgccga
     1801 gatcgccttc gacatgagtg agcgcagcgg tgtcctcatg gccaggccga gcgccctggt
     1861 gaaagccggc atcaccgcct ggctggcggc attgggcgcg aacgaggcga cggtcaggcc
     1921 ggtttcgacg ctacgggggg aaaggtgggt cggcaccgtg atgtgctcgg ccgaggggta
     1981 atgcatcatc ttctggaggc cgagctcgcc accgaaatcg aagtcgggcc ggcccacagc
     2041 ctgggaggcg gggcgtagcg cccgctcgcg ccactcgaag tcgccgccct tgatcatttc
     2101 gattcccgac ctcatcgttc cctggctcgg ctggaaggga gcgtggtaac cgagccgcac
     2161 cttgtccacg gtctcgccgg ctccgagcgc ggccgcggtc agggcggcaa gcatgtcccc
     2221 cggcacatag tcgaaaccca tcgccggaat caccgccgaa cctttcccga gggctttcgg
     2281 cccgtaggtg tcgaaggcaa gccggacgaa gggctgctcg ccggtggtgt ccagatagtg
     2341 ggtgccggcc tcgactgcgg cggccaggac cggctcgccg tggagcgaga acgggccggc
     2401 gcaggcgatc acggcgccgc tccgcgcgaa gagctccttc aggccccgcg ggtcggtggt
     2461 cgccacggca tggctctcgg gcttgccgcc ggggccgtcg aggcggtcgg ccagggcctc
     2521 cagcttctcg cggttgcggc cggcgacgat gaacggaacc tcgcgcgagg ccagctcggc
     2581 ggcgaccagc tttccggtgt agccggtggc tccgtagacg gtgatcggtc cggattgggt
     2641 gctcatgccg gggattctcg caggcagggt cgggttgccg cggggaacgg cggtagggtg
     2701 ccacgggtga gtttcgaaac gattctctac tcggtcgagg ggccggtcgc gacgatcacg
     2761 ctgaaccgtc ccgggcggct caacacgatc gtgccgccga tgcccgatga agtcgaacag
     2821 gcggtggggg aggcgacccg cgacccggag gtcaaggtga tcgtgctgcg cggggccggt
     2881 cgttcttttt gcgccggcta cgacttcggc ggcggcttcg agcactggga cgagtacctg
     2941 acgaccgatg gcgaatggga tccgggcaag gacttcgccc tggccaccgc gacggagctc
     3001 tcaccgaccc agaagttcat gagcatctgg cgcagcccga agccggtgat cgcccaggtt
     3061 cacggatact gcgtcggtgg cggcagcgat ttcgcgctct gcgcggatct gatcgtggcc
     3121 agcgaggacg cggtgatcgg caccccctac agccggatgt ggggcgccta cctttccggc
     3181 atgtggattt accggctcgg cctcgcccgg accaagtacc acgccctgac cggccggccg
     3241 ctgaacggaa agcaggcggc cgaggtcgag ctgatcaatc aggcggtccc cttcgaggag
     3301 cttgaagcga cggtggccgg gctggccgcc gatctcgctt cgattccggc ctcgcagctg
     3361 gcggcgatga agctggtcgt gaaccaggcc tacgagaaca tgggtctcgc ctcgacccag
     3421 accctcgggc cgattctcga cgggctgatg cggaacacgc cggaagcgct ggagttcatc
     3481 gacaaggcgg aatccgaagg ggtggggcgg gtgatcgagg atcgcgacgg tggcttcggc
     3541 gactactcgc aggcgccgcc ggagctccag cccgatcccc gccacatgat cgagccctga
     3601 tgccggcaag ccgcgcccgc cgcaacccgg acgagcatta cggggcatcg aaccttgagc
     3661 tcttcttcga tctcgtcttc gtcttcgcga tcacccaggt ttcgcatctg ctgctggaaa
     3721 acctgacctg gcggggtgcc ctcgaggcgg cgatggtgct gctcgtggtc tggtgggcct
     3781 ggcagtacac gacctgggcc accaacgagc ttgacaccga ccggatgccg gtccggttcg
     3841 ttctgctggt gatcatgatg gcctgcctgc tgatggcggt cgccatcccg gaggccttcg
     3901 gcgacaaggg cctgctcttc gccctctcct atgtggcgat ccagatcggc cggcaggcat
     3961 tccttgcctt cagcgccgaa ccgcacacga tcgcccgggc ccggggcctt cacatcctga
     4021 tctggttctg ctttgccgcc ccgttctgga tcgtcggcgc gctggtcgat ggcgacgccc
     4081 ggatcgcgtt ctggctggtc gccctgacga tcgactactg cggaccgttc gtgacctact
     4141 acgtcccctg gctcaagaaa gtgagccccg acgcctggaa gatcggcagc ggacacttcg
     4201 ccgagcggtt ccagctgttc acgatcatcg ccctgggg
//
LOCUS       DAOQMF010000052         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10277_length_4220_cov_3.911885, whole genome shotgun sequence.
ACCESSION   DAOQMF010000052 DAOQMF010000000
VERSION     DAOQMF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10277_length_4220_cov_3.911885"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLV77_01470"
     CDS             <1..386
                     /locus_tag="PLV77_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14547.1"
                     /translation="LFVYLVLALIGDIPHMNAWQVLVGLVGLWLFAPYVERRAGTPLF
                     LTGFVIVAGATGFLVGAVDEASGPYAISFFLPVLATAGVHIALAPRSKILCLIPVPFA
                     MTFVEIPTIAMTIIWVALEMLLTAA"
     gene            complement(489..1316)
                     /locus_tag="PLV77_01475"
     CDS             complement(489..1316)
                     /locus_tag="PLV77_01475"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNC14548.1"
                     /translation="MDLFQSIILGILQGLTEFLPISSSGHLLIFPALVGWDDPGAAFT
                     AVIQLGTEAAVLIYFRKDLWNIARDWTESIFKPEMRGELNPRMGWYLIIATIPISILG
                     LAFQDQIETAARNLWLVGSMLIIFGLVLGRADRVGTHQREVGELSFKDGVIIGLAQSL
                     ALIPGVSRSGATISAGLFLGLGREAAARFAFLLAIPAVVLSGFYELYKLLSGKESFDE
                     PMINIAVATLVAFVVGYAVIAWFLRYLTSHSMSVFVWYRVIVGTGVLILTAAGLISV"
     gene            complement(1394..2164)
                     /locus_tag="PLV77_01480"
     CDS             complement(1394..2164)
                     /locus_tag="PLV77_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC14549.1"
                     /translation="MKAESAIVFGGASGLGEATARALVEAGHKVVVADLNEEKGIELI
                     SEIGGTFVKADVTDEESVREAIAEAAGPGAGGASGGLRICVNCAGVGAAGRIVQKGEP
                     APLDGYKWAININLIGTINVLSQAAAAMVGNEPDGDGQRGVCINTASIAAYDGQIGQT
                     AYAASKGGVVGLTLPAARDLSRDGVRVMTIAPGLFDTPLLALLPEPARKALGETIPFP
                     PRLGVPEEFGALALHIVDNVMLNGEVIRLDGALRMAPK"
     gene            2205..3353
                     /locus_tag="PLV77_01485"
     CDS             2205..3353
                     /locus_tag="PLV77_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC14550.1"
                     /translation="MKRAIFEPEHQDFRETVGRFVTDEIMPNFEQWEEDGIVPRELFQ
                     KAGEKNMLAMSVSEEYGGLGLKDFRFNQIVGEEGYYQGATGAVLGVTLHNDVCLPYFE
                     EYCTDEQKERWLPGIVDGSLITAVAMTEPGIGSDLASMSTKAIRNDDHYVVDGSKTFI
                     TNGINADLIITAVKTDPKEKHRGMSIIIIERGMDGFDRGRNLEKVGMHSQDTAELFFN
                     EVKVPVENLLGNEGDGFKYLVSNLAQERMSIAVAAVATAQAALNWTLEYVKERYAFGS
                     PIGTFQNSRFQMAEIKTEVDLATVFIDRCVEALNEGTLTVEDAAEAKWWTTEMADRVI
                     DKCVQLHGGYGYMLEYPIARAYADNRVTRIYGGTTEIMKEIVGRSMGL"
     gene            complement(3489..>4220)
                     /locus_tag="PLV77_01490"
     CDS             complement(3489..>4220)
                     /locus_tag="PLV77_01490"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC14551.1"
                     /translation="VTLTNSHKGMHMKCRGKKGKALVRCQRFNFLHEAMMHSIDFHAA
                     RIAPNVAFRNVAPGESFTFEFVAGTPGVYMYHCATGPMLHHLGMGMYGMIVVDPATPR
                     PPAQEVFLVQSEFYGKVKGGYLHSSYEAMQSGTPAMVVFNGRSYRYALNPIQVGVGEP
                     VRIFFLDAGPSLFSAFHVVGTIFDSYEHDGIPDEAIHDVSTQVIAPGGAGVFELSFPE
                     AGSYPFVSHSVIDMDRGAMGMFEAS"
BASE COUNT          772 a         1382 c         1345 g          721 t
ORIGIN      
        1 ttttgttcgt ctatctggtc ctggccctga tcggcgacat cccccacatg aacgcctggc
       61 aggtcctggt cggcctggtc ggcctctggc tgttcgcccc atacgtcgag cgaagggccg
      121 gcacccccct cttcctgacc ggtttcgtga tcgtcgccgg agccaccggt ttcctggtcg
      181 gcgcggtcga cgaggcctcg ggcccctacg cgatcagctt cttccttccg gtcctcgcca
      241 ccgccggcgt ccacatcgcc ctggctcccc gatcgaagat cctctgcctg atcccggtcc
      301 ccttcgcgat gaccttcgtc gagatcccca cgatcgccat gacgatcatc tgggtcgccc
      361 tcgaaatgct cctcaccgcc gcctgaccct cctccgttca attcactcgg acatagccct
      421 aatgacttga acccaggcaa gatgcaggcg tctggaggcc agcggcggcc tcgactagca
      481 atttcgggtc agaccgagat cagaccggcg gcggtgagga tgaggacgcc ggtgccgacg
      541 atgacccggt accagacgaa gactgacatc gagtggctgg tcaggtacct gaggaaccag
      601 gcgatcaccg cgtagccgac cacgaaggcg accagggtgg cgaccgcgat gttgatcatc
      661 ggctcgtcga agctttcctt tcccgagagc agtttgtata gctcgtagaa gccggagagg
      721 acgacggccg ggatcgccag gaggaaggcg aaacgagcgg ccgcctcgcg accgagcccg
      781 aggaagagac cggcgctgat cgtggcgccg gaacgggaaa cgccggggat cagggccagc
      841 gactgggcga ggccgatgat cacgccgtcc ttgaaggaga gttcgccgac ctcccgctga
      901 tgggtgccaa cccggtcggc ccggccgagc acgaggccga agatgatcag catcgagccg
      961 accagccaga ggtttcgggc ggcggtctcg atctggtcct ggaaggcgag gccgaggatc
     1021 gagatcggga tcgtggcgat gatcaggtac cagcccatgc gggggttgag ctcccccctc
     1081 atctcgggtt tgaagatcga ctcggtccag tccctggcga tgttccagag gtccttgcgg
     1141 aaatagatca gcaccgcggc ttcggttccc agctggatca ccgcggtgaa ggcggcgccc
     1201 gggtcatccc agccgaccag cgccgggaag atcagcaggt gcccgctcga ggagatcggc
     1261 aggaactcgg tcaacccctg gaggatgccg agaatgatgc tctggaacag gtccacgact
     1321 cggagaggct atcggccgga ggcctgcccg gaccgggccg gctccgtccg ttcagctttc
     1381 gttcagatcc gccctacttc ggggccatgc gaagagcacc gtcgagccgg attacttcgc
     1441 cgttcagcat cacgttgtcg acgatgtgga gggccagcgc gccgaactcc tccggcaccc
     1501 cgaggcgggg cgggaagggg atcgtctcac cgagggcctt gcgggccggt tccggcaaaa
     1561 gcgcgagcag cggggtgtcg aagaggccgg gcgcgatcgt catcacccgc actccgtccc
     1621 gcgaaaggtc acgggcagcc ggcagggtca ggccgaccac tccgcccttt gaggcggcgt
     1681 aggcggtctg gccgatctgg ccgtcgtagg cggcgatcga tgcggtgttg atgcagacgc
     1741 cacgctggcc gtcaccgtcc ggctcgttgc cgaccatcgc cgcggcggcc tggctcagca
     1801 cgttgatggt gccgatcagg ttgatgttga tcgcccactt gtaaccgtcg agcggggccg
     1861 gctcgccctt ctggacgatc cgcccggccg cgccgacgcc ggcgcagttg acgcagatcc
     1921 tgaggccacc gctggctccg ccggccccgg ggcccgcggc ttccgcgatc gcttcgcgca
     1981 ccgactcctc gtcggtcacg tcggccttga cgaaggtgcc gccgatctcg ctgatcagct
     2041 cgatcccctt ctcctcgttc aggtcggcga ccaccacctt gtgacctgct tcgaccaggg
     2101 ccctcgcagt cgcctcgccg agtccggagg caccaccaaa gacgatcgcg ctttcagcct
     2161 tcatctgtcc tgctcctcgc tgttgttgcc atagagttgg gcgcatgaaa cgagcgatct
     2221 tcgaacccga acatcaggac ttccgtgaga ctgtcggccg cttcgtcacc gacgagatca
     2281 tgccgaactt cgagcagtgg gaggaggacg gcatcgtccc tcgcgagctc ttccagaagg
     2341 cgggcgagaa gaacatgctg gcgatgtcgg tttccgagga gtacggcggg ctcggactga
     2401 aggacttccg cttcaaccag atcgtcggcg aggagggcta ttaccagggc gcgaccggcg
     2461 cggtgctcgg cgtcaccctg cacaacgatg tctgccttcc ctacttcgag gagtactgca
     2521 ccgacgagca gaaggaacgc tggctgcccg gcatcgtcga cggcagcctg atcaccgcgg
     2581 tcgcgatgac cgagccgggg atcggctccg accttgcgag catgtccacc aaggcgatcc
     2641 ggaacgacga tcactatgtg gttgacggct cgaagacctt catcaccaac gggatcaacg
     2701 ccgacctgat catcaccgcg gtcaagaccg accccaagga aaagcaccgc gggatgtcga
     2761 tcatcatcat cgagcgcggc atggatggct tcgaccgcgg ccgcaacctg gagaaggtcg
     2821 gcatgcacag ccaggacacg gccgagctct tcttcaacga ggtcaaggtg ccggtcgaga
     2881 acctgctcgg caacgaaggc gacggcttca agtacctggt cagcaacctc gcccaggagc
     2941 ggatgtcaat cgcggtcgcc gcggttgcaa ccgcccaggc cgcgctcaac tggaccctcg
     3001 aatacgtcaa ggagcgctac gccttcggct cgccgatcgg caccttccag aactcgcgct
     3061 tccagatggc ggagatcaag accgaggtcg acctggccac cgtcttcatc gaccgctgcg
     3121 tcgaggcgct gaacgaaggc acgctcacgg tcgaggatgc ggccgaggcc aagtggtgga
     3181 ccaccgagat ggccgaccgg gtaatcgaca agtgcgtcca gctccacggc ggctacggct
     3241 acatgctcga gtacccgatc gcccgcgcct acgccgacaa ccgcgtgacc cgcatctacg
     3301 gcggcacgac cgagatcatg aaggagatcg tcggccgctc gatgggcctc taggcgcgtt
     3361 cgaagcgcca caattcaccg accttgaagt tgccggcggg tagcgctggg aacttcgagg
     3421 tctctgcccg gcagagttat acgccccggc ggctccttgg ccacgtcccg ccgccggggc
     3481 gtcctccctc aactcgcttc aaacattccc atcgccccac ggtccatatc gattaccgag
     3541 tgggatacga agggatagct gcccgcttcg ggaaacgaca gttcaaacac cccagcgcca
     3601 ccgggggcaa tgacctgggt cgacacgtcg tgaatcgcct cgtcgggtat gccgtcatgt
     3661 tcatacgagt cgaagatcgt gccgaccaca tggaaggcac tgaagagcga tggtccggca
     3721 tccaggaaga agatcctgac gggctcgccg acgccgacct gaatcggatt cagtgcgtag
     3781 cggtagcttc ggccgttgaa gaccaccatg gccggagttc cgctctgcat ggcttcgtat
     3841 gagctgtgca gatagccacc cttgaccttg ccgtagaact cgctctggac gaggaagacc
     3901 tcctgggccg ggggccgggg tgtggccgga tcgaccacga tcatcccgta catccccatg
     3961 cccaggtgat gcagcatcgg tccggtggcg cagtggtaca tgtagacgcc cggggtgccg
     4021 gcgacgaact cgaaggtgaa cgactcgccc ggggcgacgt tgcggaaggc gacattcggc
     4081 gcgatgcgtg ccgcgtggaa atcgatggag tgcatcatcg cctcatgcag gaagttgaag
     4141 cgctggcaac gcaccagggc cttgcccttc ttcccccggc atttcatatg catgcccttg
     4201 tggctgttgg tgagagtcac
//
LOCUS       DAOQMF010000053         4217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10285_length_4217_cov_3.101634, whole genome shotgun sequence.
ACCESSION   DAOQMF010000053 DAOQMF010000000
VERSION     DAOQMF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10285_length_4217_cov_3.101634"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="pstC"
                     /locus_tag="PLV77_01495"
     CDS             <1..966
                     /gene="pstC"
                     /locus_tag="PLV77_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNC14552.1"
                     /translation="QEISLNAGTVSNSETPPSGRAHRRFGDRAFSGLATAAGLAILVV
                     LAGVAAFLVSEAWPAITAPVADLPDGKSLLHYIWPLVFGTVLSSLIAILIAIPMAIAV
                     ALFTSHLAPKPIAMSVSYLTDLLAAIPSIIYGIWGIAVLGPASVPVTQWLSDHFGFIP
                     FFAGPASTTGRTMLVAGVVLALMILPIISAVAREVFQQTPKGLQESALALGATRWEMI
                     RMVVLPFGRSAVVAGSMLGLGRALGETMAVTIILSVSGVITFDLISSENPSTIAANIA
                     LQFPESSGIDVNVLIATGLVLFAITLAVNMAARAVIGRREAAGGR"
     gene            977..2011
                     /gene="pstA"
                     /locus_tag="PLV77_01500"
     CDS             977..2011
                     /gene="pstA"
                     /locus_tag="PLV77_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC14553.1"
                     /translation="MPLGEGSGVLPRRAGLLVFGISAVASALLCLVLGFSPFVWLIFT
                     GLFSLVTITVWSLAVEGRRRALDRVMTVVIVEIFVLALVPLISLVYTVVSEGLARFDV
                     QFLTEDMRGVVGEGGGAFHAAMGTFIITAFATVAAVPVGVLAAIYLVEYGKGRLKRSI
                     TFFVDVMMGIPSIVAGLFAFALFALFFGPGIRLGIMGSVALMVLMIPIVIRSAEEMLR
                     VVPNSLREASYALGVPKWLTVLRVVLPTALAGIVTGVMLAVARVIGETAPLLVTTGSI
                     DTTNLDPFDGRMANLPVYVYEQYKFPGVPPEAFIERAWAGALLLILIVMVLFLAARLI
                     SRRYGVEIRR"
     gene            2122..2901
                     /gene="pstB"
                     /locus_tag="PLV77_01505"
     CDS             2122..2901
                     /gene="pstB"
                     /locus_tag="PLV77_01505"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC14554.1"
                     /translation="MAKRIDAEDLNIYYGSFLAVKDCTVSIHPNKVTALIGSSGCGKS
                     TFLRSLNRMHELIDGARVEGRVLLDGMDIYSPSMDPVLVRQQIGMVFQQPNPFPTMSI
                     YENTAAGLRLNRRRLKKTEIDGIVERSLRGAHLWDEVKDRLEKPASGLSGGQQQRLCI
                     ARATAIEPEVLLMDEPCSALDPIATLAIEDLINELKKRYTIVIVTHNMQQAARASDYT
                     GFFNIEKSGDPGVLVEIDETEKIFSNPSLQETEDYVSGRFG"
     gene            2981..3889
                     /locus_tag="PLV77_01510"
     CDS             2981..3889
                     /locus_tag="PLV77_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14555.1"
                     /translation="MLCAAIDIGSNTTRILVAEPDEGKLKKVMEQRAYTRIGQARLDD
                     GTIPAEKVAEVAEVVDTQVKLARELGAGTFRAVATAALRDAPNGKEAAAEIEAVAGIP
                     VHVVSENEEGRLAFLGATKALGYQVEGRVAVVDVGGGSTEIIIGTADGGVEQVRSWPI
                     GSGQLAEELISSDPPSASEIRRVRDRVDEVFAEVQITQPEQAVAVGGSATSLRKIVGA
                     RLEYETLERSIRVLSGDEALEVGRQFELDPIRVRMLATGVLILEKVSELLGQDLQIGK
                     GGIREGIVLDMLNGGIDVSEPALTPG"
     gene            3918..>4217
                     /locus_tag="PLV77_01515"
     CDS             3918..>4217
                     /locus_tag="PLV77_01515"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNC14556.1"
                     /translation="MSTESDPRFRLAASQVLEERATQISVLAKGVLDVTETGAADELW
                     LASRRLRAALEVFRSCFGRAQYREGRAEARRISRAAGARRDIDVVIAAYESVGEET"
BASE COUNT          677 a         1408 c         1367 g          765 t
ORIGIN      
        1 caggagattt cattgaacgc gggcaccgtc tcaaacagcg aaacaccacc ttcaggcagg
       61 gctcaccgcc ggttcggtga ccgtgccttt tccggtcttg ccactgcggc cggcctggcg
      121 atcctggtcg tgctggccgg ggtcgcggcc tttctggtca gcgaggcctg gcccgcgatc
      181 accgctccgg tcgccgacct tcccgacgga aagtccctcc tccactacat ctggccactc
      241 gtcttcggca ccgttctttc ctcgctcatc gcgatcctga tcgcgatccc catggcgatc
      301 gcggtggcgc tcttcaccag tcacctcgcc ccgaagccga tcgcgatgtc ggtctcctac
      361 ctgaccgacc tgctggccgc gatccccagc atcatctacg gcatctgggg gatcgccgtg
      421 ctcgggccgg cgtcggtgcc ggtcacccag tggctttccg accacttcgg cttcattccc
      481 ttcttcgccg gccccgcctc gaccaccgga cgcaccatgc tggtcgccgg ggtggtgctg
      541 gccctgatga tcctgccgat catcagtgcg gtcgcccgcg aggtcttcca gcagaccccg
      601 aaagggctcc aggaaagcgc cctcgccctc ggcgcgaccc gctgggagat gatccgcatg
      661 gtcgtgctgc ccttcggccg ctcggccgtg gtcgccggct cgatgctcgg tctcggccgg
      721 gcgctcggcg agacgatggc cgtgacgatc atcctctcgg tctccggggt gatcaccttc
      781 gacctgatca gctccgagaa cccttcgacg atcgccgcca acatcgcgct ccagttcccg
      841 gagtcatccg ggatcgacgt caacgtcctg atcgccaccg gcctcgtgct attcgcgatc
      901 accctggcgg tcaacatggc ggctcgggcc gtgatcggca gaagggaggc cgccggtggc
      961 cggtagtgcc gtatcgatgc ccctgggtga aggctccggg gtcctgcccc gccgggccgg
     1021 tttgctcgtc ttcgggatct cggccgtcgc ctcggcgctg ctctgcctgg ttctcggctt
     1081 cagcccgttc gtctggctga tcttcaccgg gctcttcagc ctggtcacga tcaccgtctg
     1141 gtcgctggcg gtcgaaggcc gtcgccgggc actcgaccgg gtcatgaccg tggtcatcgt
     1201 cgagatcttc gtgctcgccc tggtgccgct gatttcactc gtttacacgg tggtcagcga
     1261 agggctggcc cgcttcgacg tccagtttct gaccgaggac atgcggggtg tggtcggtga
     1321 aggcggcggc gcctttcacg ccgcgatggg caccttcatc atcactgcct tcgccaccgt
     1381 cgcggccgtg ccggttggcg tgctggccgc gatctacctg gtcgagtacg gcaaggggcg
     1441 cctcaagcgt tcaatcacct ttttcgtcga cgtgatgatg ggcatcccct cgatcgtcgc
     1501 cggcctcttc gccttcgccc tgttcgcgct gttcttcggc cccggcatcc ggctcggaat
     1561 catgggctcg gtcgccctga tggtgctgat gatcccgatc gtgatccgct cggcggaaga
     1621 gatgctgcgg gtcgttccga actcgctgcg ggaagcttcc tacgccctcg gcgtgcccaa
     1681 gtggctgacc gtcctgcggg tcgtgctgcc gaccgccctg gccgggatcg tcaccggcgt
     1741 catgctggcg gtggcccgcg tgatcggcga gaccgcaccg ctgctggtca ccaccggatc
     1801 gatcgacacg acgaaccttg acccgttcga cggccggatg gcgaacctgc cggtctatgt
     1861 gtacgagcag tacaagtttc ccggcgttcc gcccgaggcc ttcatcgaaa gggcctgggc
     1921 cggggcgctc cttttgatcc tgatcgtgat ggtgctgttc cttgcggccc ggctcatcag
     1981 tcgacgctat ggagtggaga tccgccgatg aaccagaccg agactatgaa ggagaaagag
     2041 atggttgacg cccaggccgg accggccgac cccatcatgg accccccggc cagcccgagt
     2101 gccaccgcga ccgtggacgc cctggcgaaa cggatcgacg ccgaggacct gaacatctac
     2161 tacgggagct tcctcgcggt aaaggactgc acggtcagca tccacccgaa caaggtgacc
     2221 gcgctgatcg gctcttctgg ttgcggcaaa tcgaccttcc tcaggtcgct gaaccgcatg
     2281 catgagctga ttgacggcgc ccgggtcgaa ggccgggtcc tgctcgacgg gatggacatc
     2341 tactcgccgt cgatggatcc ggttctggtc cgccagcaga tcggcatggt cttccagcag
     2401 ccgaacccgt ttccgaccat gtcgatctac gagaacacgg cggcgggcct gagactgaac
     2461 cgccgcaggc tgaagaagac cgagatcgac gggatcgtcg aacgctcgct tcgcggcgcc
     2521 cacctctggg acgaagtcaa ggaccggctc gaaaagccgg ccagcgggct ctccggcggc
     2581 cagcagcagc gcctctgcat cgcccgcgcg acggccattg aaccggaagt cctgctcatg
     2641 gacgagccct gttcggccct ggatccgatc gctaccctgg cgatcgagga cctgatcaac
     2701 gaactgaaga agcgctacac gatcgtgatc gtcacccaca acatgcagca ggcggcccgg
     2761 gccagcgatt acaccgggtt cttcaacatc gagaaaagcg gtgacccggg cgtcctggtc
     2821 gagatcgacg aaaccgagaa gatcttctcg aacccgtcgt tgcaggagac cgaggattac
     2881 gtcagtggcc gtttcggttg agcttccggt cccgccggag ggccgaaggc tccgaaccgg
     2941 cccgtccgct tcgcctgcca gaatcgtttg ggtaaagacg atgctctgcg cagcgatcga
     3001 cataggttcg aacaccaccc ggatcctggt cgccgagccc gatgaaggca agctcaagaa
     3061 ggtgatggag cagcgggcct acaccaggat cggccaggcc cggctggatg acggcacgat
     3121 tccggccgaa aaggtcgccg aagtcgccga agtggtcgac acccaggtga aacttgcgcg
     3181 cgaactcggc gccgggactt tccgggcggt cgccacggcg gcactgaggg acgctccgaa
     3241 cgggaaggaa gccgcggccg aaatcgaggc ggtggcgggc attccggttc acgtggtcag
     3301 cgagaacgag gaaggccggc tcgccttcct cggggcgacc aaggcgctcg gctatcaggt
     3361 cgaaggccgg gtggccgtgg tcgacgtcgg tggcggttcg accgagatca tcatcggcac
     3421 ggccgacggc ggggtggaac aggtccgctc ctggccgatc ggctcgggcc agctggccga
     3481 ggaattgatc agctcggacc cgccctcggc ttccgagatc cgccgggtgc gggaccgggt
     3541 cgacgaagtc ttcgccgagg tccagatcac ccagcccgag caggccgttg cggttggcgg
     3601 ttcggccact tcactgcgca agatcgtcgg cgcccggctc gaatacgaga ctctggaacg
     3661 cagcatccgg gtcctcagcg gtgacgaagc gctcgaagtc ggccgccagt tcgaactcga
     3721 tccgattcgc gtccgcatgc tggcgaccgg ggtcctgatt ctcgagaagg tctccgaact
     3781 gctcggccag gacctgcaga tcggcaaggg cgggatccgc gaagggatcg tgctcgacat
     3841 gctgaacggc ggaatcgacg tttcagaacc cgcattgaca cccggctgag tcggcctgag
     3901 gtctagggtt ccggttgatg agcaccgaat ccgatccccg cttccgcctt gccgcgtcgc
     3961 aggtgctcga agagcgggcg acccagatct cggtccttgc caagggtgtg ctcgacgtga
     4021 ccgagaccgg agcggccgac gaactctggc tcgcttcccg tcggcttcgc gccgcacttg
     4081 aggtctttcg ctcctgtttc ggccgggccc agtatcgcga gggaagggcg gaagccaggc
     4141 ggatttcgcg ggccgccggg gcgaggcgcg acatcgacgt ggtgatcgcc gcctacgaaa
     4201 gcgtcggtga ggaaacc
//
LOCUS       DAOQMF010000054         4165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10499_length_4165_cov_3.832360, whole genome shotgun sequence.
ACCESSION   DAOQMF010000054 DAOQMF010000000
VERSION     DAOQMF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10499_length_4165_cov_3.832360"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2892)
                     /locus_tag="PLV77_01520"
     CDS             complement(<1..2892)
                     /locus_tag="PLV77_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HNC14557.1"
                     /translation="MTEFAPSSATRDGLYDSTNEHDACGVAMVARLDNEPRHEVVSQG
                     LLALENLEHRGAAGADASTGDGAGILIQLPDLYYREEAGVDLPPAGQYGVAMCFLPRE
                     NEERREELKTLITETTEAEGQVVLGWRDVPVDPGHVGNTANDSRPHVAQLFIGAGESH
                     RSDQDAFERKLYVIRRVCEIAAGEELYIASCSSRTIVYKGMLTSYQVPGFYPELQDER
                     VRSAMALVHSRFSTNTFPSWDLAHPFRLIAHNGEINTLRGNINWMRARESQLASELFG
                     DDLKKVTPVVRPDGSDSATFDNVLELLMLAGRSLPHAAMMMIPESYQGRTDLSDELKG
                     FYAFHSCLMEPWDGPAAVCFTDGRVIGATLDRNGLRPGRWVETNDGMVVLGSEIGLLD
                     IAPEDIKRLGRLQPGKLFLVDLEQKRIIEDEEVKREVATQQPYGEWFENASVHFDDLP
                     TAYVTMTGIQPTPRRQQAFGYTQEDLRVLITPMAATGAEPIGSMGNDVALPVLSDNRP
                     PLYSYFKQLFAQVTNPPIDPIREEIVMSLSAGVGAERNLLDESEQHAHQLCLDQPILM
                     NQELETIRHVNHDIFRADTIDITWDVAEGTAGIESRLDAICEEAEEAIEKGFNILILS
                     DRRTGPRRVPIPALLAVGAVHHHLVRAGTRLRAGLILESGEPREVHHMATLIGYGCSA
                     INPYLMLDSVDDLVVNGLVPDMQSPEKAQRNVVKAIGKGLLKTISKMGISTTQSYGGA
                     QIFEAVGIEKEVIDKYFTGTASRIGGIGLDVIAQETMDRHASAFPGAGDDLLPVGGVY
                     AWRRDGEFHQWNPETVSLVQHAVRGEGGSAQEKYDEFAQQVNEIALKRATLRGLFKLK
                     AKDSGPVPIEEVEPAKEIVKRFATGAMSLGSISRESHENLAIAMNRLGGKSNTGEGGE
                     DPVRFTPEENGDSRRSAIKQVASGRFGVTFHYLANADQLQIKMAQGAKPG"
     gene            complement(3047..3910)
                     /locus_tag="PLV77_01525"
     CDS             complement(3047..3910)
                     /locus_tag="PLV77_01525"
                     /inference="COORDINATES: protein motif:HMM:NF005878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC14558.1"
                     /translation="MEHSLEGAVVVVTGGAAGIGRATATKMAESGANVAIGDIHAEKA
                     AETAEEITSRVSAGGSGSGGGAIGRPLDVTDRDGFEIFLDGVEAEFGPIDCLVNNAGV
                     MLLGPVDQESPAATKAMIDVNLLGVINGTQAAMDRMKPRARGRIVNIASQAGKAGMAG
                     GATYCATKFGVIGFSEAVRAELKGTGVSISWVLPGLVNTELSAGLPDIPLMDLLSPDD
                     IADAVLKALTRGGSEIWVPARNQWLDAPARLLPRPMREKVFAMTGADKVLAEADPDTR
                     QDYEDRHSGVS"
     gene            3970..>4165
                     /locus_tag="PLV77_01530"
     CDS             3970..>4165
                     /locus_tag="PLV77_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNC14559.1"
                     /translation="MSPVLAAVDSVAGVGLVTAFIAGFVSFLSPCVLPIVPGYLSAVS
                     GVSVNELDDAGWKKVLTPSLI"
BASE COUNT          636 a         1354 c         1390 g          785 t
ORIGIN      
        1 gccgggcttg gcgccctggg ccatcttgat ctggagctgg tcggcgtttg cgaggtagtg
       61 gaaggtgacc ccgaaccggc cggaagcgac ctgcttgatc gcggagcggc gcgagtcgcc
      121 gttttcttcc ggggtgaacc ggaccgggtc ctccccgccc tcgccggtgt tcgacttgcc
      181 gccgagtcgg ttcatggcga tcgccaggtt ctcgtggctt tcgcgggaga tcgagccgag
      241 cgacatggcg ccggtcgcga accgcttcac gatttcctta gcgggttcga cttcctcgat
      301 cggaaccgga cccgaatcct tcgccttcag cttgaagagc ccgcgcaggg tggccctctt
      361 cagggcgatc tcgttgacct gctgggcaaa ctcgtcgtat ttctcctggg cggagccacc
      421 ttcgccgcgg accgcgtgct ggaccagtga caccgtctcc gggttccact ggtggaactc
      481 gccgtcacga cgccacgcgt acacaccgcc gaccggcagc aggtcgtcgc cggctccggg
      541 gaacgccgag gcgtgacggt ccattgtctc ctgggcgatc acgtcgagac cgatgccgcc
      601 gatcctcgag gccgtgccgg tgaagtactt gtcgatcact tccttctcga tgccgaccgc
      661 ttcgaagatc tgggcgccac cgtaggactg ggtggtggaa atgcccatct tcgagatcgt
      721 cttcagaagg cccttgccga tcgccttgac tacattgcgc tgggccttct ccggcgactg
      781 catgtccggc acgagaccgt ttacgaccag gtcgtcgacc gagtcgagca tcaggtaggg
      841 gttgatcgcc gagcagccgt agccgatcag ggtcgccatg tgatggacct cgcgcggctc
      901 gcccgactcg aggatcaggc ccgcccgcag gcgcgtgccg gcccggacca ggtgatggtg
      961 aaccgcgccg accgcgagca gggccgggat cgggacgcgg cgcgggccgg tccggcggtc
     1021 ggaaaggatc aggatgttga agcccttctc gatcgcttcc tcggcctctt cgcagatcgc
     1081 gtcaaggcgg gactcgatgc cggccgtgcc ttcggcgacg tcccaggtga tgtcgatcgt
     1141 gtccgcgcgg aagatgtcgt ggttgacgtg gcggatcgtc tcgagttcct ggttcatcag
     1201 gatcggctgg tcgagacaga gctgatgggc gtgctgctcg ctttcgtcga gcaggttgcg
     1261 ttcggcgccg accccggccg agaggctcat cacgatttcc tcgcggatcg gatcgatcgg
     1321 cgggttggtg acctgggcga agagctgctt gaagtagctg taaagcgggg gacgattgtc
     1381 ggagaggacc ggcagcgcga catcgttgcc catcgagccg atcggttcgg caccggtcgc
     1441 ggccatcggg gtgatcagga cccgcaggtc ctcctgggtg tagccgaagg cctgctggcg
     1501 acgcggggtc ggctggatcc cggtcatcgt cacatacgcg gtcggcaggt cgtcgaaatg
     1561 gaccgaggcg ttctcgaacc attcgccgta gggctgctgg gtggcgactt cccgcttgac
     1621 ttcctcgtcc tcgatgatcc gcttctgctc gagatcgacg aggaagagct tgccgggctg
     1681 caggcggccg aggcgcttga tgtcctcggg ggcgatgtcg agcaggccga tctcggagcc
     1741 gagcacgacc atgccgtcat tggtttcgac ccagcggccg ggcctcagac cgttgcggtc
     1801 gagcgtggcg ccgatcacgc ggccgtcggt gaagcagacc gccgccggtc cgtcccacgg
     1861 ctccatcagg caggagtgga acgcgtaaaa gcccttcagc tcgtcactca ggtccgtccg
     1921 gccctggtag gactcgggga tcatcatcat cgccgcatgc ggcagcgagc gaccggccag
     1981 catcagcagt tcgagcacgt tgtcgaaggt cgccgagtcg gagccgtccg gccgcacgac
     2041 cggagtcacc ttcttcaggt cgtcaccgaa cagctcggag gcgagctgcg attcccgggc
     2101 ccgcatccag ttgatgttgc cccgcagggt gttgatctcg ccgttgtggg cgatcaggcg
     2161 gaagggatgg gccaggtccc agctggggaa ggtgttggtg gaaaagcgcg agtgaaccag
     2221 ggccatggcc gagcggaccc gctcgtcctg gagctccggg taaaagcccg gcacctggta
     2281 gctggtcagc attcccttgt agacgatggt gcgggaggaa caggaggcga tgtaaagctc
     2341 ttcgccggcg gcgatttcgc agacccgccg gatcacgtag agcttgcgct cgaaggcgtc
     2401 ctgatcgctc ctgtgtgact cgcccgcccc gatgaagagc tgcgcgacat ggggccggga
     2461 atcgtttgcc gtgttgccga cgtggccggg atcaaccgga acgtcccgcc agccgagcac
     2521 gacctggccc tcggcttcgg tggtctcggt gatcagggtc ttgagttctt cccggcgctc
     2581 ttcgttttcg cgcggcagga agcacatcgc gaccccgtac tggccggctg gcggcaggtc
     2641 cacgccggct tcctcgcggt agtagaggtc gggcagctgg atcaggatgc cggcgccgtc
     2701 accggtcgag gcatcggccc cggcggctcc acggtgctcg aggttctcga gggccagcag
     2761 gccctggctg accacttcat gacggggctc gttgtccagg cgcgcgacca tggcgacgcc
     2821 acaggcgtcg tgttcatttg tagagtcgta gaggccgtcg cgggtcgcgg acgacggggc
     2881 aaattcagtc ataagcgcgc ttttcaggcc gcggatgcgg cgtttggttg tcggagacac
     2941 gtattcgcag cggtcggaac cgctgtccga ggtgggccag cccaccgggg cagtttaccg
     3001 aaggtcaggc ccgctcatct atccggcggg ccaaaccggc cgggcttcag gagactcccg
     3061 aatgccggtc ctcgtagtcc tggcgggtat cgggatcagc ttcggccagg accttgtcgg
     3121 cgccggtcat cgcgaaaacc ttctcgcgca tcggccgcgg cagcagcctc gccggggcgt
     3181 ccagccactg gttgcgggcc ggaacccaga tctcgctgcc accccgggtc agggccttca
     3241 acaccgcgtc tgcgatgtca tccggggaaa gaaggtccat caggggaatg tctggcaaac
     3301 cggcggagag ctcggtgttg accaggccgg gcagcaccca ggaaatggaa accccggttc
     3361 ccttcagctc ggcccgcacc gcctcggaga agccgatcac gccgaacttg gtcgcgcagt
     3421 aggtggcgcc accggccatt cctgccttgc cggcctgcga ggcgatgttg acgatccggc
     3481 cccgggcccg tggcttcatc cgatccattg cagcctgggt gccgttgatc accccgagca
     3541 ggttcacgtc gatcatcgcc ttcgtggcag ccggactttc ctgatcgacc ggcccgagca
     3601 gcatcacccc ggcgttgttg acgaggcagt cgatcggccc gaactcggcc tcgactccat
     3661 ccagaaagat ctcgaagccg tcgcggtcag tgacgtcaag cggccggccg atcgcacccc
     3721 cgccgctccc gctcccgccg gccgaaaccc ttgaggtgat ctcttccgct gtttcggcgg
     3781 ccttctctgc gtggatgtct ccgatcgcca cattcgcgcc tgattcggcc atcttcgtcg
     3841 cggtggcccg gccgattccg gcggcaccac cagttaccac gacaactgca ccctccaggc
     3901 tgtgttccat gggtgccaaa ctactctcta atccccttgc catttgcaga actgaattcc
     3961 gcgggcctcg tgagcccggt acttgccgcc gttgactccg tcgccggggt cgggctggtc
     4021 accgcgttca tcgccggctt cgtttccttc ctctccccct gcgtgctgcc gatcgttccc
     4081 ggctaccttt ctgcggtctc cggggtttcg gtcaacgagc tcgatgacgc cggctggaag
     4141 aaggtgctca ccccgagcct catct
//
LOCUS       DAOQMF010000055         4159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10523_length_4159_cov_3.783138, whole genome shotgun sequence.
ACCESSION   DAOQMF010000055 DAOQMF010000000
VERSION     DAOQMF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10523_length_4159_cov_3.783138"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /gene="rsmH"
                     /locus_tag="PLV77_01535"
     CDS             complement(<1..953)
                     /gene="rsmH"
                     /locus_tag="PLV77_01535"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNC14560.1"
                     /translation="MTAAARLAPIGAKVSDSRQSHNPFDRPEQGPRGHLPVLAGELVD
                     LIGPQLGETVIDCTFGAGGHARLVAERIGAEGTLIAIDRDPVAQAHFQAFETEAACRT
                     RFVAAEFSQALKYLQNEGIRASALYMDLGMSSLQVDAAERGFSYSHDAPLDMRMDPRQ
                     ELTAEVIVNTWSEQQLAQMLRELGEERYAGGISRQIVARRPLTSTSDLVEAIKAGMPA
                     AARFGSGHPARRSFQALRIAVNGELEAVDAALPIAWDSLEIGGRMAVISFHSLEDRRI
                     KRFFNDLATGCTCPPELPVCVCGHEPEAEVLTRRPISAGEE"
     gene            complement(950..1387)
                     /gene="mraZ"
                     /locus_tag="PLV77_01540"
     CDS             complement(950..1387)
                     /gene="mraZ"
                     /locus_tag="PLV77_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNC14561.1"
                     /translation="MAFRGQHEHSLDSKDRLTVPRKFRAALDEGYVLAKGPDACLWLY
                     STKDFDAMEERYVAPHSPFGADARNLRRMINASADEGNLDAAGRIRIPDNLKELAGIE
                     GACTVVGAGDYIEIWNEGTWAKLSQDLLSQFGDVAENLAGSTE"
     gene            1600..2490
                     /locus_tag="PLV77_01545"
     CDS             1600..2490
                     /locus_tag="PLV77_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14562.1"
                     /translation="MTEISDANQLRGAKFKELMSKAKTWIIIVVCSVIAAAAGMIVGP
                     AIAGGAFVLVFLIGIVITFMIADSRAATAFYEAYAQARGLTHQSGGILGGSTPLLRKG
                     DRTRVDQQFNGMLSEGIEGTLALYTYTVTSTDSKGNETSTDYPFTVIMVNMPGTVQHL
                     TDLRVQRQSGLKLFEKFEDAFRGDFGRVTLESEAMRDRYEIFVRKEQDPVWVRRLFSP
                     SFIVWLTDSPPKKFAFELENGWLCAYVPKHRDDTEGFDEMIRLGTHVAGRLEEEVGQS
                     SPAEAHHDSPEVKIEVEEEK"
     gene            2487..3401
                     /locus_tag="PLV77_01550"
     CDS             2487..3401
                     /locus_tag="PLV77_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14563.1"
                     /translation="MSEEAKPGGTFEGASAEGEVAVTHANQLRSAKFREMTRKKSTWA
                     WIIGLSVLAGLIFAGLGFPAMGGAAFVIVFLGSLLVVFMIADSRAEDAFYDYYCETHG
                     LTRSTSPEIGELTPLLRMGDKSNTDEMFSGELSPGMDGNLVLYTYTEVHRDSDGDETE
                     TDYPFTIIHVEMPEIVQHMTDLQVHRAGFKLLDGIQDKFLGNMQRVTLESEAFAKRFE
                     VFVNDSQDPIWTRRLFSPSFIVWLTESPPDSFAFELENGHLVAYVSRHMDSTESLENV
                     TRVGTYVANRLLQEVAESSPKAARETGS"
     gene            3398..>4159
                     /locus_tag="PLV77_01555"
     CDS             3398..>4159
                     /locus_tag="PLV77_01555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC14564.1"
                     /translation="MNPAKGVRPQAILALASVASVALNMWIGRGITFSTDELVWFMLS
                     PDLDLSGAFHPYVGHLILTSKALYKLIFETIGPDYRVIRLAAAMVLVGVVWILYRLLR
                     RYCGEWPALAISLVVLFFSGDPAHVFHGNGITVLGSVGLGLFALLMLSEPATRRSVLA
                     CLALCLGVATYSQTLPFLVGTTVFLIASKRIRDLWVPAIPFLGYFAWWIWARDLPGSS
                     ESSLEPERIFMLPVWGFRATGAVAESLIHLPDGVAS"
BASE COUNT          708 a         1385 c         1302 g          764 t
ORIGIN      
        1 tcttcctccc cggcggaaat cggccggcgg gtgagcacct cggcctccgg ctcatgcccg
       61 cagacgcaga ccggcagctc cggcgggcag gtgcagccgg tcgcgagatc gttgaagaag
      121 cgcttgatcc ggcggtcttc gagcgagtgg aacgagatga cggccatgcg accgccaatc
      181 tcgaggctgt cccaggcgat cggcagggcc gcatcgactg cttcgagttc accgttgacc
      241 gcgatccgaa gcgcctggaa gctgcggcgg gccggatggc cggaaccgaa acgggccgcg
      301 gccggcatcc cggccttgat cgcctcaacc aggtccgaag tgctggtcag cggccgccgg
      361 gcgacgatct ggcggctgat cccgccggcg tatcgctcct cgccgagttc acgcagcatc
      421 tgggcgagct gctgctcgga ccaagtgttg acgatcactt cggcggtcaa ttcctgtcgc
      481 ggatccatcc gcatgtcgag gggcgcgtca tgtgagtagg agaagccacg ctcggcggcg
      541 tccacctgaa gtgaagacat cccaagatcc atgtacagcg ccgaagcgcg aataccttca
      601 ttttgcaggt atttcagagc ctggctgaac tcggcggcga cgaatctcgt ccggcaggcg
      661 gcctcggtct cgaacgcctg gaagtgagcc tgggcgaccg ggtcacggtc gatcgcgatc
      721 agggtacctt cggctccgat ccgctcggcg accaggcggg cgtgaccgcc ggccccgaat
      781 gtgcagtcga tcaccgtctc gccgagctgc gggccgatca ggtcgaccag ctccccggcc
      841 agtaccggca ggtgccccct cggcccctgt tcgggccggt cgaaagggtt gtggctctgg
      901 cgcgagtcgc tcaccttggc tccgatcggg gcgagccgcg cggcggctgt cactccgtgc
      961 tcccggcgag gttctcggcc acgtcgccga actggctgag caggtcctgg ctcagtttgg
     1021 cccaggtccc ttcgttccag atctcgatgt agtcaccggc gccgaccacg gtgcaggcgc
     1081 cctcgattcc ggccagctcc ttgaggttgt ccggaatgcg gatgcggccg gccgcatcga
     1141 gattgccctc gtctgcgctg gcgttgatca tccggcgaag gttgcgcgcg tcggccccga
     1201 acgggctgtg gggagcgacg tagcgctctt ccatcgcgtc gaagtctttg gtcgagtaga
     1261 gccagaggca ggcatccggg cctttggcga gaacgtatcc ctcgtcgaga gcggcacgga
     1321 acttgcgggg caccgtgagg cggtccttgg aatcgaggct gtgttcgtgc tggcctctga
     1381 acgccaccgc tcctcgtcct ttctttgcct cgccgtgggt tgaccgggaa agagttgggc
     1441 cggggtggtg agagcgggag gagctccatc accccggccc ttggcttcaa ctcgatggca
     1501 atgtaaccca ctttgcccca ctttgcaaca caacccccca ctttcttcca aaagttgcaa
     1561 cggcgattgc accgcccggt catgaaataa cctcccgata tgaccgagat ctccgatgcg
     1621 aatcagctgc gcggcgccaa gttcaaggag ttgatgagca aggcgaagac ctggatcatc
     1681 atcgtcgtct gcagcgtgat cgccgctgcc gccgggatga tcgtcggccc ggcgatcgcc
     1741 ggcggcgcct tcgttctcgt cttcctgatc gggatcgtga tcactttcat gatcgccgac
     1801 agccgggctg ccaccgcgtt ctacgaggcc tacgcccagg cacgcggact gacccatcag
     1861 tccggcggca tcctcggcgg ctccaccccg cttctgcgga aaggtgaccg gacccgggtc
     1921 gaccagcagt tcaacggcat gctctccgag gggatcgagg gaaccctcgc cctctacacc
     1981 tacacggtta ccagcaccga ctcgaagggc aacgagacat cgaccgacta ccccttcacc
     2041 gtgatcatgg tcaacatgcc gggcaccgtt cagcacctca ccgacctcag ggtgcagagg
     2101 caatcgggtc tgaagctgtt cgagaagttc gaggacgcct tccgcggcga cttcggccgg
     2161 gtcaccctgg aaagcgaggc gatgcgggac cgctacgaga tcttcgtcag gaaggaacag
     2221 gatccggtct gggtccggcg tctgttctcg cccagcttca tcgtctggct gaccgacagc
     2281 ccgccgaaga agttcgcctt cgaactggag aacggctggc tctgcgccta tgtgcccaag
     2341 catcgcgatg acaccgaagg cttcgacgag atgatcaggc tcggtaccca tgtggcgggc
     2401 cgcctcgaag aggaagtcgg ccagagctca cctgccgaag cgcatcacga ctcgcccgaa
     2461 gtcaagatcg aagtggaaga agagaagtga gcgaggaagc gaagcctggc ggcacctttg
     2521 aaggtgcgag cgccgaaggc gaggtggcgg tcacccacgc caaccagctc cgctcggcca
     2581 agttcaggga gatgacccgg aagaagtcga cctgggcctg gatcatcggg ctttcggtcc
     2641 tcgccggact gatcttcgcc gggctcggct tcccggcgat ggggggcgcc gccttcgtca
     2701 tcgtcttcct cggctccctg ctggtggtct tcatgatcgc cgacagccgg gccgaagacg
     2761 ccttctacga ttactactgc gaaacgcacg gcctgacccg gagcacctcg cccgagatcg
     2821 gcgaactgac cccgctgctc cgcatgggtg acaagtcaaa cacggacgag atgttctccg
     2881 gcgagctttc gcccgggatg gacggcaacc tcgtgctcta tacctacacc gaggtccatc
     2941 gcgattcgga cggtgacgaa acggaaaccg actacccctt cacgatcatc cacgtcgaga
     3001 tgccggagat cgtccagcac atgaccgacc ttcaggtcca ccgggcgggc ttcaagctcc
     3061 tcgacgggat ccaggacaag ttcctcggca acatgcagcg ggtgaccctc gaaagcgaag
     3121 cctttgccaa gcggttcgag gtgttcgtca acgacagcca ggacccgatc tggacccggc
     3181 gcctcttctc ccccagcttc atcgtctggc tgaccgaaag cccgcccgac agtttcgcct
     3241 tcgaactcga gaacggccat ctggtcgcct acgtctcgag gcacatggac agcacggaaa
     3301 gcttggagaa cgtgacccgg gtcggcacct acgtggccaa ccggttgctc caggaagtcg
     3361 ccgagtcctc gccgaaggcc gcgcgcgaaa ccgggtcgtg aacccggcaa agggggtccg
     3421 gccccaggcc atcctggcgc tggcctcggt ggccagcgtc gccctcaaca tgtggatcgg
     3481 ccgggggatc accttcagca ccgacgagct ggtctggttc atgctcagtc ccgatctcga
     3541 cctttccggc gccttccacc cctacgtcgg ccacctgatc ctcacctcga aagccctcta
     3601 caaactgatc ttcgagacga tcgggccgga ctaccgggtg atccgcctgg ccgcggccat
     3661 ggttctggtc ggggtcgtct ggattcttta ccggctgctc cgccgctact gcggcgagtg
     3721 gccggccctc gcgatcagcc tggtcgtcct ttttttcagc ggcgaccctg cccatgtctt
     3781 ccacggcaac ggaatcaccg tgctcggttc tgtcggcttg ggcctcttcg ccctgctgat
     3841 gctgagcgaa ccggcgaccc ggcggtcggt gctggcctgc ctcgcccttt gtctaggtgt
     3901 cgccacctat tcgcagacgc tgcccttcct ggtcggaacc accgttttcc tgattgcctc
     3961 gaaacggatc cgggaccttt gggttccggc gatcccgttc ctcggatatt tcgcctggtg
     4021 gatctgggcc cgggacttgc ccggttcctc ggagagctca ctggaaccgg aacggatctt
     4081 catgctgccg gtctggggct tccgggccac cggcgccgta gccgagagcc tgattcacct
     4141 gccagacggg gtcgcctcc
//
LOCUS       DAOQMF010000056         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10663_length_4131_cov_4.655790, whole genome shotgun sequence.
ACCESSION   DAOQMF010000056 DAOQMF010000000
VERSION     DAOQMF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10663_length_4131_cov_4.655790"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..635)
                     /locus_tag="PLV77_01560"
     CDS             complement(153..635)
                     /locus_tag="PLV77_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14565.1"
                     /translation="MTSKIKKILASLLALAALALGGAAIASATNGGSDDDHGAQEKAE
                     VNDHDSDANENESTSEGADDNEAEDGQEAADPGEAVSSTVAAKAEAAAVARTGGTVQE
                     VTAETNDAPEKGDDQDPGEKAAPAGTAYEVDLTKGSQELKVSLDRQFNVLQVQTGQDD
                     "
     gene            795..1460
                     /locus_tag="PLV77_01565"
     CDS             795..1460
                     /locus_tag="PLV77_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC14566.1"
                     /translation="MNLNVLVVEDNPELRRLLNQGLDEEGFEVDLARNGREALMLADP
                     QKHDLLVIDIGLPDSDGRDVCQALKSKGIEAPVLFLTAKDSGPDRVSGFSAGGDDYLT
                     KPFVFAELVARLRVLGRRLTRGDRSTSGDLRLDPVRHVLTCGDRETELTPTEFRLTGA
                     MIARSGEVIRRREMIAAAWPPGAIVHDNTVDVYVRRLRRKLDELESESRIHTVHGVGY
                     RLE"
     gene            1457..2803
                     /locus_tag="PLV77_01570"
     CDS             1457..2803
                     /locus_tag="PLV77_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNC14567.1"
                     /translation="MKGTIRQRLTFSVVCVTAVMLALLVVGFNLSLRASLDGDVDRLL
                     HARAQATLDNIDLENGKLEVSEGSDDGAEDALVWIYDDRKPLESPQVASQLDRVAASL
                     AAEGSGERDDSDSDTRLFARPVVQNGRVVGTVVTGVSLEPYERTADRAAFASVVLALI
                     MLVLITITTRLVVGRALKPVSEMTEEAAKWSEHDLDRRFNEGEPTDELTRLAATFDTM
                     LDRMAYMVRHERNFSAELSHELRTPLSAISAEAEIALTKDRDAAEYRESLERIAERST
                     ELTKILETLLDVARSEGSGGSDEAADLLDSVDSAVAAAGSLTDRYGVVVELDRPEGRI
                     RAQVAGDTFQRMISPIIENAITYARATVSIAIRSRGRTAEVVIRDDGPGFSPRDLEAA
                     FEPGMRGIAARNEAAPPGTGLGLALARRLARANGGDVSIPVSGDGGTVILTVPIAS"
     gene            complement(2816..3610)
                     /locus_tag="PLV77_01575"
     CDS             complement(2816..3610)
                     /locus_tag="PLV77_01575"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14568.1"
                     /translation="MLNKVPEVTLYFWIIKVLCTTVGETAADYMNVDLGFGLTNTTYA
                     IGALLVVALIFQFRSRRYVPGIYWTAVVLISVVGTLITDNLVENMNVALQTTTIGFSV
                     ALAITFVVWYAFERTLSIHSITTTRREAFYWLAILFTFALGTSAGDQIAEQYGLGYFV
                     SILIFAAAIGLVALAHFRFRLGAVTAFWVAYILTRPLGASIGDWMSQPTANGGLGLGT
                     TVTSFIFLAAILGLVIYLSVTKVDQTPPEVVEAEMEETEEAEAVSV"
     gene            complement(3716..3789)
                     /locus_tag="PLV77_01580"
     tRNA            complement(3716..3789)
                     /locus_tag="PLV77_01580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3753..3755),aa:Thr,seq:tgt)
     gene            complement(3884..>4131)
                     /locus_tag="PLV77_01585"
     CDS             complement(3884..>4131)
                     /locus_tag="PLV77_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNC14569.1"
                     /translation="GPTALDPVNQAISSEELSSLVSCLQSTLSEMESSVLGYYLEDRS
                     YEDVAALLGCETKTVDNALQRVKRKVSKHLESRDIAV"
BASE COUNT          750 a         1245 c         1382 g          754 t
ORIGIN      
        1 cccgacgtcg agaaggcagt cagcatcgtc tgcctgctgg tcgactgagc ggctgcgtcg
       61 ggatcttcag caacgtcgac cggcacgggt aaacgcggca agagaagccc gggccggagt
      121 cgacccgggt tcctcctccg cccttgacac gttcagtcat cctgcccggt ctgaacctgg
      181 agcacgttga actgcctgtc gagcgagacc ttcaactcct gagagccctt ggtcaggtct
      241 acttcatagg cggtaccggc cggagcggct ttctcgcccg ggtcctgatc gtcgcccttc
      301 tcgggcgcgt cattggtctc ggcggtcact tcctggaccg ttccgccggt cctggcaacc
      361 gcggcggctt cagccttcgc ggcgaccgtg cttgaaacgg cttcaccggg gtcggctgct
      421 tcctgaccgt cttcggcttc gttgtcgtca gcgccttcag aggtggactc gttctcgttg
      481 gcgtcagagt cgtgatcgtt gacttcagcc ttctcctgcg cgccgtgatc gtcgtcggag
      541 ccgccgttgg ttgcagaagc gatcgcggca ccgccgaggg ccagagccgc aagcgccagg
      601 agtgacgcga gtattttctt gatcttggat gtcatgtgtt cttccttctc atcggggccg
      661 aggggcatcg gcgcttttcc aatgaggcga gtatgtgcgc ggaaccctga atttctcctg
      721 accgggagcg gattttcagg agtcgttcag gtttgccacc gatcattgga ccgtatgacc
      781 gaagcggcga tcacatgaac ctgaacgtcc tggttgtcga ggacaacccc gagctccggc
      841 ggctgctcaa ccaggggctc gacgaggagg gattcgaggt cgatctggcg cgcaacggtc
      901 gcgaagccct gatgcttgcc gatccgcaaa agcacgacct gctggtgatt gacatcgggt
      961 tgccggattc cgatggccgc gatgtctgcc aggcgctcaa gtcgaagggg atcgaggcac
     1021 ccgtgctgtt cctgaccgca aaggattccg gaccggaccg ggtctcgggc ttcagcgccg
     1081 gtggagacga ctacctgacc aagccgttcg tgttcgccga actcgtggcc cggctgcggg
     1141 tgctcggcag gcgtttgact cggggcgatc ggtccacttc cggggatctc aggctggatc
     1201 cggtccggca cgtactgacc tgcggggacc gtgagaccga actgactccg accgagtttc
     1261 gcctgaccgg ggccatgatc gcccgatcgg gagaagtgat ccgacgccgc gagatgatcg
     1321 cggccgcctg gccaccgggg gcgatcgtgc acgacaacac ggtcgatgtc tacgtgcgga
     1381 ggcttcgcag gaaactcgac gaactcgaga gcgagtcgag aatccacacc gttcacggag
     1441 ttggataccg gctcgaatga aagggacgat ccgtcagcga ctgactttct cggtggtctg
     1501 cgtgaccgcg gtgatgctgg ccctgctcgt ggtgggcttc aacctctcgc tgcgtgccag
     1561 tctcgatggc gacgtggacc ggctccttca cgccagggcc caggcgacgc tcgacaacat
     1621 cgatctggag aacgggaaac tcgaagtcag cgagggatcg gacgacggcg ccgaagacgc
     1681 gctggtctgg atctacgacg accgaaagcc gcttgagtct ccgcaggttg ccagccagct
     1741 ggatcgggtg gcggcctcac tcgccgcgga gggaagcggg gagcgggatg actcggacag
     1801 cgacacaagg ctcttcgcgc ggccggtggt gcagaacggc cgggtagtgg gcacggtggt
     1861 taccggggtc tcgctcgagc cttacgagcg gaccgcggat cgggccgcct tcgcttcggt
     1921 ggtgctcgcc ttgatcatgc tcgtgctgat cacgatcacg accaggctcg tggtcggcag
     1981 ggcactcaag cccgtctcgg agatgaccga ggaagccgcg aaatggagcg agcacgatct
     2041 ggaccgaagg ttcaacgagg gcgagcccac cgacgagctg accaggctgg cggccacttt
     2101 cgacacgatg ctggaccgga tggcctacat ggtccggcac gagaggaact tttcggccga
     2161 actctcgcat gaactcagga ctcctctttc cgcgatatcg gccgaggcgg agatcgcgct
     2221 cacaaaggat cgggacgcgg ctgaataccg ggaatcactg gaacggatcg ccgagcgatc
     2281 gaccgagctc acgaagattc tcgagacctt gctcgacgtc gcgagatcgg aaggcagcgg
     2341 cggatccgat gaagcggccg atctccttga ctcggtcgat tccgcagtgg ccgcggcggg
     2401 ttcgctgacc gatcgatacg gcgtcgtggt cgagctggac cggcccgaag gccggatccg
     2461 ggctcaggtg gccggcgaca cgttccagcg gatgatctcg ccgatcatcg agaacgcgat
     2521 cacctacgcc agggccaccg tgtcgatcgc aatccggtcg aggggaagaa cggccgaagt
     2581 cgtgatccgg gatgacggac ccgggttttc gccccgggat ctggaggcgg cgttcgagcc
     2641 cgggatgcgg ggaattgcgg cacgaaacga agcggcgccg cccggtaccg gtctgggcct
     2701 cgcccttgcc cgtcgcctgg cccgggccaa cggtggagac gtctcgatcc ccgtgtcggg
     2761 cgacggagga acggtcatcc tgaccgttcc gatcgcgtcc tgagttggcg cctgactaaa
     2821 cgctgaccgc ctccgcctcc tccgtctcct ccatttcggc ctcgacgacc tccggtggag
     2881 tctggtcaac ctttgtgacc gagaggtaga tcacgagccc gaggatcgcg gccaggaaga
     2941 tgaagctggt cacggtggtt ccaaggccca gacccccgtt cgcggtcggt tgggacatcc
     3001 agtcaccgat tgaagctccg agcggtctgg tcaggatgta ggcgacccag aaggctgtaa
     3061 cggcccccag cctgaacctg aagtgcgcca gcgcgacgag cccgatcgcc gcggcaaaaa
     3121 tcaggatgga aacgaaataa ccgagcccgt actgctcggc gatctggtcg ccggccgagg
     3181 ttcccagcgc gaaggtgaac agaattgcga gccagtagaa ggcctctctg cgggtggtcg
     3241 taatcgagtg gatcgagagg gtccgctcaa aggcgtacca cacgacaaag gtgatcgcca
     3301 acgccaccga gaacccgatc gtcgtggttt ggagcgcgac attcatgttc tccacgaggt
     3361 tgtcggtgat caacgtgcca accacgctga tcaggaccac ggcagtccag tagatccccg
     3421 gcacgtagcg tctggatctg aactggaaga tcagggctac gacgagcaag gcgccgatcg
     3481 cataggtggt attggtcaac ccgaagccaa ggtcgacgtt catgtaatcg gccgcggttt
     3541 ctccgaccgt ggtgcagaga accttgatga tccagaagta gagggttacc tccggaacct
     3601 tgttgagcat ctggcgggcc agagaccggt ccgccccggt tgtcgggagt ctttgagcaa
     3661 ggctgttcat gccggaagaa tccactcccg aacctgaacg actcctgaca gcgagagccc
     3721 ggctccgggc tcgaaccgga aacctcctgt ttacaagaca ggtgctctag ccaattgagc
     3781 tagccgggca ggaggcgtga ttctgacagc cccggcaggc tttggccccg gccgggtgcg
     3841 gtccggcgcg gggcgatggc ctgtcaggcc gaatgccgct tgttcagacg gcgatgtcgc
     3901 gtgattcgag gtgcttgctg accttgcgct tgacccgctg gagggcgttg tcgacggtct
     3961 tggtctcgca accgagcagg gcggcgacgt cctcgtagga gcggtcctcg aggtagtagc
     4021 cgagcacgct gctttccatt tccgagaggg tcgactggag gcaggaaacg agggaggaga
     4081 gttcttcgga agaaatcgcc tggttgaccg ggtcgagcgc ggtcggcccc g
//
LOCUS       DAOQMF010000057         4121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10713_length_4121_cov_3.636990, whole genome shotgun sequence.
ACCESSION   DAOQMF010000057 DAOQMF010000000
VERSION     DAOQMF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10713_length_4121_cov_3.636990"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLV77_01590"
     CDS             complement(<1..510)
                     /locus_tag="PLV77_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNC14570.1"
                     /translation="MSTEQQTEAPAEGDGGGVAAGNDTLSVTDNRTGRTYELPIEDDT
                     IKALDLRQIKVHEDDFGMMAFDPAFTNTASCRSSITYIDGDAGILEHRGIPIDQLCEH
                     STFLEVAYLLVFGELPTRPQLEAWVHDVTHHTFVHEDIKKFLTGFRYDAHPMGMLLAS
                     TGALSTFYPD"
     gene            complement(566..1477)
                     /locus_tag="PLV77_01595"
     CDS             complement(566..1477)
                     /locus_tag="PLV77_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC14571.1"
                     /translation="MSLTVVGSIAFDAVATPFGERERMLGGSAVHFSLAASFFTDVRV
                     VGPVGDDFTETELAVLENRGVITDDIERVSGETFFWKGHYEYDLNTAHTDDTRLNVFG
                     DFEPKLSDTSKAADLLFLANIQPDLQRQVRAQCPDAGFVGLDTMNLWIDTAKESLEAA
                     ISEVDCLLINDAEIRQLTGEPNLARAAAAVMEMGPKAVIAKQGEYGAALFTPGGFFAL
                     PGFPLEDVRDPTGAGDSFAGGFLGYLDGEAGGIDQQTLRTAMGYGTVLASYNVEEFGT
                     ERVSRLSRDEIESRLEALRSMTSFSLT"
     gene            1516..2463
                     /locus_tag="PLV77_01600"
     CDS             1516..2463
                     /locus_tag="PLV77_01600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HNC14572.1"
                     /translation="MKAIQIQEFGGPEVMNLVELPDPVPAEGEVLVEINRAGVNFSDT
                     HITNDQYVARQQLPLIPGIEFVGVAEGGRRVAAVVPNGSYAEKIAVPESSLVEIPDEI
                     DDEQAAGIMIQGVTADGVLTISGNLKPGETVVVGAAAGGTGSLAVQLARSMGAGKVIG
                     LASGEEKQRLVLDLGADAAVDSRSERLAEEVTAAAGGQVDVVLEMAGGNSFDQLIETL
                     RPFGRMVVVGIATREQNELRTGRLLKNSLSVTGFWLMHLLARPDLARDSIDRVFGATA
                     RGDLKAVVGGTYGLSEVRRVHQDIAGRKTVGKLLLDPTR"
     gene            2502..4019
                     /locus_tag="PLV77_01605"
     CDS             2502..4019
                     /locus_tag="PLV77_01605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC14573.1"
                     /translation="MGLSPEIQQAIDEIGFDKPSPIQAEAIPELLAGHDVIGQAQTGT
                     GKTAAFGLPMLQYLDPANDEVQAIVLTPTRELCIQVTQALRSYAEHLDIEIVAVFGGA
                     PIRSQQAQLRSGAHVVVATVGRMKDLISRRSLVLTAARFVVLDEADEMLDLGFIEDVE
                     RILRMCPSGRQTALFSATMPPPIKRLADTFMYDPVTISITPKTLTVDAIEQAFVEVPG
                     LAKVDKLIEVVKEEDPEQAILFCRTKIGASRLERDLKNRGLDVKALHGDLSQGVRDGV
                     MLAFKNHRCKLLVATDIAARGLDVDHVTHVINYDIPNSSETYVHRIGRTGRAGRTGRA
                     ITFVTPDQRRDLEQIKKDVKASISEWEKPEDRIEHARPPRRKRRGEQPAPLDDQPAVA
                     VEEVEEFDEVVEVPLEGPGGDQPETASDDIPDAREGAVKLFVNRGSRSDITEEDLRWA
                     LREGAVLSDDQISDVRVLERFSFVEVDGDVAEKTVEFLDGTKLKGSGIRVEIARS"
BASE COUNT          759 a         1270 c         1400 g          692 t
ORIGIN      
        1 atcagggtag aaggtcgaaa gcgcaccggt cgaggcgagc agcatgccca tcgggtgggc
       61 gtcgtagcgg aagccggtga ggaacttctt gatgtcctcg tgaacgaagg tgtgatgggt
      121 gacgtcatgg acccaggctt cgagctgcgg gcgtgtggga agctcgccga agaccagcag
      181 gtaagccact tcgaggaagg tcgaatgctc acaaagctgg tcgatcggga tgccgcggtg
      241 ctcgaggatt ccggcatcgc cgtcgatgta ggtgatcgag gagcggcagg aggccgtatt
      301 ggtgaaggcc ggatcgaagg ccatcatccc gaagtcgtct tcgtggacct tgatctggcg
      361 cagatccagc gccttgatcg tgtcgtcctc gatcggaagc tcgtaggtgc gaccggtccg
      421 gttgtcggtg accgagaggg tgtcgtttcc ggcggctact ccgcccccat cgccctcggc
      481 gggcgcttcg gtttgctgtt cggtcgacat gcttctcctc ggatcctgtt ctctgatctt
      541 tcttcggacc agtatctcag gtcggttaag tcagcgagaa actggtcatc gaccgcagcg
      601 cttcgaggcg cgactcgatc tcgtcgcggc tcaggcggct gactctttcg gtgccgaact
      661 cctcgacgtt gtaggaggcg agcacggtgc cgtagcccat cgcggtgcgc agagtctgct
      721 ggtcgatgcc gccggcctcg ccgtcgaggt aaccgaggaa gccgccggcg aaggaatcac
      781 cggcgccggt cgggtcgcgc acgtcctcca ggggaaaccc gggcagggca aagaagccgc
      841 cgggcgtgaa cagggcggcg ccgtactccc cctgcttggc gatcacggcc ttcgggccca
      901 tctccatcac cgcggcagcg gcccgggcga ggttcggctc tccggtcagc tggcggatct
      961 cggcgtcgtt gatcagcagg cagtcaacct cggatatcgc cgcttccagc gactccttcg
     1021 cggtgtcgat ccagaggttc atcgtgtcga gcccgacgaa accggcatcc ggacactggg
     1081 cccggacctg gcgctgcagg tccggctgga tgttggccag aaagagcagg tcggccgcct
     1141 tcgaggtgtc cgagagcttc ggctcgaaat cgccgaagac gttgaggcgg gtgtcatcgg
     1201 tgtgggccgt gttcaggtcg tactcgtagt gacccttcca gaagaaggtc tcgccgctga
     1261 cccgttcgat gtcatcggtg atcacgccgc ggttttcgag cactgccagt tcggtctcgg
     1321 tgaagtcatc cccgaccggc ccgaccacgc ggacatcggt gaagaaactc gcagcaagcg
     1381 agaagtggac tgcggagccg ccgagcatgc gctcgcgctc gccaaatggt gtggcgaccg
     1441 catcaaaggc gatcgacccg acaacggtca gagacatggg cgggatcgta tccgccaccg
     1501 tgaaaagatg gctttgtgaa agcgatccag attcaggagt tcggcgggcc ggaggtgatg
     1561 aacctggtcg aactgcccga tccggtgccg gccgaagggg aagtcctggt cgaaatcaac
     1621 cgggcgggag tcaacttctc cgacactcac atcaccaacg accagtacgt ggcgaggcaa
     1681 cagctgccgc tgatcccggg aatcgagttc gtgggcgtgg ccgagggtgg ccgtcgggtt
     1741 gcggcggtgg tcccgaacgg cagctacgcc gagaagatcg cggtgccgga aagttctctg
     1801 gtggagatcc ccgatgaaat cgatgacgag caggccgccg gaatcatgat ccagggagtc
     1861 accgccgacg gggtactgac catctccggc aacctgaagc cgggcgagac agtggtggtc
     1921 ggtgcggccg ccggcgggac cggcagcctc gcggtccagc tggcccgctc gatgggcgcc
     1981 ggaaaagtga tcgggctggc ctcgggggag gagaaacagc ggctggttct agacctcggc
     2041 gcggacgccg ccgtcgactc gcgcagcgag cgcctggccg aggaggtgac cgcggcagcc
     2101 ggcggccagg tcgatgtggt gctggagatg gcgggcggca attccttcga ccagttgatc
     2161 gaaaccctgc gcccgtttgg ccgcatggtg gtggtcggaa tcgccacccg ggagcagaat
     2221 gagctcagga ctggcagact gctgaagaac agcctcagtg ttaccggctt ctggctgatg
     2281 cacctgctgg cccgccctga tctcgcccgc gactcgatcg accgggtttt tggcgctacc
     2341 gcccggggag atctgaaggc agtggtcggc ggaacctacg ggctttccga agtccgccgg
     2401 gtccatcagg acattgccgg acggaagaca gtcggcaagc tgctgctgga cccaaccaga
     2461 tgacagaaca agaacagaca gaagagaaac ttttcaagga cttgggcctc tcgcccgaga
     2521 tccagcaggc gatcgacgag atcggctttg acaagccgag cccgatccag gccgaagcca
     2581 tcccggagct cctcgcgggg cacgacgtga tcggccaggc ccagaccggt accggcaaga
     2641 ccgcggcctt cgggctgccc atgctccagt acctcgaccc ggccaacgac gaggtgcagg
     2701 caatcgtcct cactccgacc cgtgagctct gcatccaggt gacccaggcg ctccgttcct
     2761 acgccgagca tctcgacatc gagatcgtcg cggtcttcgg cggggcgccg atccgcagcc
     2821 agcaggctca gctccgctcc ggggcgcatg tggtcgtagc gaccgtgggg cggatgaagg
     2881 acctgatttc ccgccggtcc ctggtcctca ccgcggcccg cttcgtggtg cttgacgagg
     2941 ccgacgagat gctcgacctc ggtttcatcg aggacgtcga gcggatcctc cggatgtgcc
     3001 cgagcggccg tcagaccgcc ctattctcgg ccaccatgcc gccgccgatc aagcggctgg
     3061 ccgacacctt catgtacgac ccggtgacca tctcgatcac cccgaagacc ctcaccgtcg
     3121 acgcaatcga gcaggccttc gtcgaggtgc cggggctggc caaggtcgac aagctgattg
     3181 aggttgtcaa agaagaggat cccgagcagg cgatcctctt ctgccgcacc aagatcgggg
     3241 cttcgcggct ggagcgtgac ctgaagaacc gcggcctgga cgtcaaggcg ctgcacggcg
     3301 acctcagcca gggagtccgc gacggagtga tgctggcctt caagaaccat cgctgcaagc
     3361 ttctggtcgc gaccgacatc gcggcccggg ggctggatgt cgaccacgtc acgcacgtga
     3421 tcaactacga catcccgaac agttccgaga cctacgttca ccggatcggg cgtaccggcc
     3481 gggcggggcg taccggccgg gcgatcacct tcgtcacccc ggaccagcga cgggacctgg
     3541 agcagatcaa gaaggacgtg aaggcgtcga tctccgaatg ggagaagccc gaggaccgga
     3601 tcgagcacgc ccggccgccc cgccgcaagc gtcgcggcga gcagccggct ccgcttgacg
     3661 accaaccggc agtggcggtc gaggaggtcg aggaattcga cgaggtggtc gaggttccgc
     3721 tcgagggtcc aggcggcgac cagccggaaa ccgcttccga tgacatcccg gacgcgaggg
     3781 agggtgcggt caagctcttc gtcaaccggg gttcccgctc ggatatcacc gaggaagacc
     3841 tgcgctgggc cctgagggaa ggcgctgtcc tcagtgatga ccagatctcc gacgtgcggg
     3901 tgctggaacg cttttccttc gtcgaggtgg acggcgacgt ggccgagaag acggtcgagt
     3961 tcctcgacgg caccaagctc aagggttcag gcatccgggt cgagatcgcc cgcagttagg
     4021 accacctaca gcggctcacg gagattcgct ggtcaggcga tcgattactt cgaaaagccg
     4081 ttcgaaattg attggcttgg ccagccgggc atcggccgaa t
//
LOCUS       DAOQMF010000058         4112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10756_length_4112_cov_3.684003, whole genome shotgun sequence.
ACCESSION   DAOQMF010000058 DAOQMF010000000
VERSION     DAOQMF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10756_length_4112_cov_3.684003"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLV77_01610"
     CDS             complement(<1..308)
                     /locus_tag="PLV77_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14574.1"
                     /translation="MARVEDTLHDREIRQAGAIGEAGTLRDEEITVSDFTTELAERIP
                     GVEFELEPEQEMLTVNMGPHHPATHGVLRLVVDLEGEVVKDLKPVIGYVHTGIEKSCE
                     T"
     gene            complement(308..967)
                     /locus_tag="PLV77_01615"
     CDS             complement(308..967)
                     /locus_tag="PLV77_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNC14575.1"
                     /translation="MPDAPGLELILAEINREHPGAVLDTSYDREQAALIIDPSKVIEV
                     LTWLKQTPGQEYTFLSSVHGADHFPLTPRFAVHYELLNRERYERLRVKAVIPDPGAAP
                     TEVSVVAPGSSDAGAVDSDSGERGSSMEMPETEGRSTVLDGETLPVVPSVVELFPTAD
                     FQEREVHDFFGIVFEGHPDLRRILMPEDYVGWPQRRDFPVGGEPVIFTRDEVTNPGWW
                     Q"
     gene            complement(960..1574)
                     /locus_tag="PLV77_01620"
     CDS             complement(960..1574)
                     /locus_tag="PLV77_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNC14576.1"
                     /translation="MLRGDLTGEDLDKYVEERVVTTTLEKVLNQARAHSVFPATFGLA
                     CCAIEMMSTVSPRYDIARFGAEAFRASPRQADLLILSGRVSIKMAPVVRRIYDQMLEP
                     KWAISMGACSSSGGMFQNYAVVQGADKFMPVDVHVPGCPPRPEALLYGFTKLQQKIMG
                     NPDPGWRSRYNAHGTEEWARGGPASRPSEEALEAYAKAREVLGA"
     gene            complement(1661..2041)
                     /locus_tag="PLV77_01625"
     CDS             complement(1661..2041)
                     /locus_tag="PLV77_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNC14577.1"
                     /translation="MLHQYLPVLVFGALGIIVGGAFATLNLLLGPKKVKGDESSAAVR
                     QLDPYECGLPSEISKTFRFGVSFYLVAMLFILFDIEVVFLYPVGAILKSTDSVFVLAE
                     VILFVALLFVALIYVWRKGALDWR"
     gene            2194..2673
                     /locus_tag="PLV77_01630"
     CDS             2194..2673
                     /locus_tag="PLV77_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14578.1"
                     /translation="MSAAYTAAGLAVIALNLLAAVVGAVSWQRNVPSVAFWYLLRAAQ
                     IATGFFVLFACVVYATGYRASDELHYLYVFLPVAVSFLAELMRGASASQELGDRLSPR
                     EPKTSQELGELFAGLDPDRQQTIGLAIIRRETAVMTLASFVIAFLIWRALDTTAGMF"
     gene            complement(2938..3942)
                     /locus_tag="PLV77_01635"
     CDS             complement(2938..3942)
                     /locus_tag="PLV77_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC14579.1"
                     /translation="MDARRRVSPAELNRKLGQVVKSGHGVITGDDLREIGVSRQTIRR
                     RKESGLLIPVLRDVFTLPGCRPDEIGRRKAAVHAGGSSCCLSHRSALAHHGLIYDQLP
                     VHVLRTGGADRRNGSALKRSRDFGFSVLSHQTRNLPPDEITIIEGIRVATPERALLDF
                     AATGRGSDIGKVLTQGERKGNLCWGRLESILENSRGRRGVTTLREEMDLFDPESVNSE
                     SDPEEDFLRMIRRRSLPMPLVNVWLAPYRVDFLWWHLMLIVELDPYGTHRGKRSFHGD
                     RRKSVELETRGFRVIRLTEKDLYAFEERTARDLETIMEAQAALLGVSVWPAGQNAATR
                     "
BASE COUNT          716 a         1317 c         1326 g          753 t
ORIGIN      
        1 gtctcgcagg acttctcgat gccggtgtgg acgtagccga tcaccggctt caggtccttg
       61 accacctcgc cctcgagatc gaccacgagc cgcagcacgc cgtgggtcgc ggggtgatgc
      121 gggcccatgt tgaccgtgag catctcctgc tcgggttcca gttcaaactc gactccgggg
      181 atccgctcgg ccagttcggt cgtgaagtcg gagaccgtga tctcttcgtc gcgcagggtg
      241 ccggcctcgc cgatcgcccc ggcctgccgg atttcacggt cgtgaagtgt gtcttcgacg
      301 cgcgccatca ctgccaccag cccgggttgg tcacttcgtc acgggtgaag atcaccggct
      361 cgccaccgac cggaaagtcc cggcgctgcg gccagccgac gtagtcctcg ggcatcagga
      421 tccggcgcag gtcggggtgg ccttcgaaga cgatgccgaa gaagtcatgg acctcccgct
      481 cctggaaatc ggccgtcggg aagagctcaa ccacggacgg tacgaccggg agagtctctc
      541 catcgaggac cgtcgagcga ccttccgttt ctggcatctc catcgatgag ccccgttcac
      601 cggaatcgga atcgaccgcc cccgcgtcgc tgctcccggg cgcgacaacc gagacctcag
      661 tgggcgcggc ccccgggtcc gggatgacgg ccttcacgcg gaggcgttcg tagcgttcgc
      721 ggttgaggag ttcgtagtgg accgcgaagc gcggggtgag ggggaaatgg tcggcgccgt
      781 ggacggagct gaggaaggtg tactcctggc ccggcgtctg cttgagccag gtcaggacct
      841 cgatcacctt cgaggggtcg atgatcaacg ccgcctgctc ccggtcgtag ctcgtgtcga
      901 gcacggcccc gggatgttcg cggttgatct cggcgaggat gagttccagt ccgggcgcgt
      961 caggcaccga ggacctcccg ggccttcgcg taggcctcga gtgcttcctc cgaaggtcgt
     1021 gaagccgggc cgccgcgggc ccactcctcg gttccgtggg cgttgtagcg actgcgccag
     1081 cccggatcgg gattgcccat gatcttctgc tgaagcttgg tgaacccgta aagcagtgcc
     1141 tcgggccggg gcgggcagcc gggcacgtgg acgtcgaccg gcatgaattt gtcggctccc
     1201 tgaaccaccg cgtaattctg gaacattccg ccggaggagg agcaggcgcc catcgagatc
     1261 gcccacttcg gttccagcat ctggtcgtag atgcggcgga cgaccggcgc catcttgatc
     1321 gagacccggc cggaaaggat cagcaggtca gcctggcggg gcgacgcccg gaaggcttcg
     1381 gcaccgaaac gggcgatgtc atagcgcggt gaaacggtcg acatcatctc gatcgcgcag
     1441 caggcgagac cgaaggtcgc ggggaagacc gaatgggcac gggcctggtt gaggaccttc
     1501 tcgagggtgg tcgtgacgac tctctcctcg acgtacttgt cgaggtcttc gccggtcagg
     1561 tcgccgcgca gcatgtcgcg ggccttgagt tggcgggcgc ggagctcttc cggtgacttg
     1621 ggctgctcga ggggggcgtg aacccgaagc ttctggcgtt ctatctccaa tccagggctc
     1681 ccttccgcca gacgtagatc agtgccacga agagcagggc cacaaagagg atcacttcgg
     1741 ccaggacgaa gaccgagtcc gtcgacttga ggatcgcccc caccgggtag aggaagacga
     1801 cttcgatgtc gaacaggatg aagagcatcg ccaccaggta gaagctgacc ccgaagcgga
     1861 aggtcttcga gatctccgaa ggcaggccgc actcgtaggg atccagctgg cggaccgcgg
     1921 cgctggactc gtcccccttc accttcttcg gccccagcag caggttcagg gtcgcgaaag
     1981 caccaccgac gatgatgccg agggcgccga agacgaggac tgggaggtac tgatggagca
     2041 ttggtgggag gtgggtggtc ggttcgtcag ggaactaagg tgtccgctgt ctatgggtta
     2101 tacacctccg cgccccgact ttgtatcaag tcgttacata gtgatttttc gcataaaaaa
     2161 gcggaatccc gaccatatgg ggcgggatta gcagtgtctg ccgcctatac cgctgcgggt
     2221 ctcgccgtga ttgcgctcaa cctgctcgcc gcggttgtcg gggctgtctc ctggcaaagg
     2281 aacgttccat cggtcgcctt ctggtacctg ctgcgggccg cccagatcgc caccggcttc
     2341 ttcgtcctct tcgcctgcgt cgtctacgcg acgggctacc gggcaagcga cgagctccac
     2401 tacctctacg tgttcctgcc ggtcgcggtc tcgttcctgg ccgagctgat gcgtggcgca
     2461 tccgccagcc aggaacttgg ggaccggctc agcccgcggg aaccgaagac cagccaggag
     2521 ctcggcgagc tcttcgccgg actcgacccg gaccgccagc agacgatcgg cctggcgatc
     2581 atccgccgcg aaaccgcagt gatgactctc gccagtttcg tgatcgcctt cctcatctgg
     2641 cgcgccctgg ataccaccgc cggaatgttc taaaccctcc cctcgaagca tcctgaaacg
     2701 aggcaggcgc cagtggaggc actcgcctcg tgtcggcacc cacgtcgcgg ccacactcac
     2761 ctggtgccgg cgtcctgaca cgaggcaggc gccagttgcg acgctcccgt cgtggcggca
     2821 ctcgcctcgt gtcggcaccc acgccgcggc cacactcacc tcgtgccggc gtcctgacac
     2881 gaggcaggcg ccagtcgcgg ggctcgcgtc gtgtcgggac tcgcctcgtg tcggggctca
     2941 cctcgtggcg gcattctgtc ccgccggcca gaccgagacg ccgagtagcg ctgcctgggc
     3001 ctccatgatg gtctcgaggt ccctcgcggt tctttcctcg aacgcgtaga gatccttctc
     3061 ggtcagcctg atgaccctga agccccgggt ttcgagttcg acactctttc gacgatcgcc
     3121 gtggaatgac ctcttgcccc gatgcgttcc atacggatcg agttcgacga tcagcatcaa
     3181 gtgccaccaa aggaagtcga cccggtatgg ggccagccag acattcacca ggggcatcgg
     3241 aagggaccgc cgccggatca ttcgaagaaa gtcttcttcc gggtcggact cggagttgac
     3301 tgattcggga tcgaaaaggt ccatttcttc ccgcagcgtg gtcactcccc ggcggcctcg
     3361 agagttctca aggatcgact cgagcctgcc ccagcacagg tttcccttgc gctccccttg
     3421 ggtcaggacc tttcctatgt ccgatcccct gccagtcgcg gcaaaatcga gcagtgcacg
     3481 ctccggagtc gcgacccgga ttccctcgat gatcgtgatc tcgtctggcg gaagattgcg
     3541 cgtctgatga ctgaggacgg aaaacccgaa atcccgactt cgcttgaggg ctgaaccatt
     3601 tctgcggtcc gcgccaccgg tccggagcac atggacggga agttggtcgt agatcaggcc
     3661 gtgatgggcg agagcgctcc gatgcgaaag gcagcagcta ctcccgcctg cgtgaaccgc
     3721 ggcctttcgg cgcccgattt cgtcaggccg gcagccgggc agggtgaaca catcgcgaag
     3781 gacgggtatc aaaagcccgg actcctttct gcgacgaatg gtctgcctgg agactccgat
     3841 ttccctcagg tcatcaccgg tgatcacccc gtgacctgac ttcacgacct ggccaagctt
     3901 cctgttcagt tcggccgggc tgacccttcg tcgtgcatcc atgggaaaag catgaagcgg
     3961 aaaagctcac acatggctcg acaggagcaa caattcagta acaggaaacc gccgaagccc
     4021 gggcggttgc cgaagcctgg gcggtaccga agcctgggcg gtaccgaagc ctgggcggtt
     4081 gccgaagcct gggcggttgc cgaagcctgt gc
//
LOCUS       DAOQMF010000059         4112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10757_length_4112_cov_3.631255, whole genome shotgun sequence.
ACCESSION   DAOQMF010000059 DAOQMF010000000
VERSION     DAOQMF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10757_length_4112_cov_3.631255"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..483)
                     /locus_tag="PLV77_01640"
     CDS             complement(190..483)
                     /locus_tag="PLV77_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14580.1"
                     /translation="MSEPEAGEGDESTEVEQSPVPARGERFGFDLGNPVERESAAFSW
                     LIVVIIAAVAVGAVGKLVSPLAAVILVLILLGIVSVPIFRGLRHGLGSPDDDE"
     gene            complement(491..1117)
                     /locus_tag="PLV77_01645"
     CDS             complement(491..1117)
                     /locus_tag="PLV77_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HNC14581.1"
                     /translation="MESASIAAHGHVDDHHHGPPEAHQSARIDRTILGILLFILSEVM
                     LFGAFFAAYFFLRVVADPASWPPEGVELPVLIAGINTCILVSSSFTVHYALEAIKHNN
                     HRGLKLGLTTTWLLGATFLFIQINEYIHIGFTPRDGAFGSIFFSLTGLHGAHVFVGLL
                     LLTFANIRAYRGHFGPEQKDHLGVEVPGIYWHFVDIMWIIVFLAVYII"
     gene            complement(1120..2025)
                     /locus_tag="PLV77_01650"
     CDS             complement(1120..2025)
                     /locus_tag="PLV77_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14582.1"
                     /translation="MSKPLLIFLNEVAGGRKLLEAAREKAADASYVVVVAPQNQPSVG
                     QLFDPEERAEAARARVDVTLAVLDEFGIEAIGEVLDPEYDLALGDAIRSHRPGEVLLS
                     CTYDFRFGLTRRDLLSWAEITYGDVTNITHIPVRIEDDSISLNVTHTLVVANRTLTSE
                     DLQERLLDRSGDRPHRYTIIAPAADDVSEATVARNLATVMAKLYHADIDATGQPMSPD
                     PFDAVKNAIEHYRVDEILISTLSGEESHWLKEDLIGRVREITEKPVVHHEAGRPPEAV
                     AAVVAEGEAPEAAGAEDSTAEENEA"
     gene            complement(2022..3935)
                     /locus_tag="PLV77_01655"
     CDS             complement(2022..3935)
                     /locus_tag="PLV77_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC14583.1"
                     /translation="MAAAIRQPGWYRAAIGTVLGAAFGFFLVVGLRLLSGLDAFQTEQ
                     TGYPQVIVPLITTPFGFLFGFGAFDYWFRWAAGKPTIPEDHSGHGATRWQDYFTFNTD
                     HKVIGTQYVVTTLFFFLIGGLLALLMRAELAQPGGQIVAANTFNGLFSTHAVVMIFLF
                     IIPIFAGLANYVLPLMIGAPDMAFPRLNALSFWLLPIAGIIFLGSFLAPGGSFDAGWT
                     GYAPLSTGAPLGQSFFNIGVQFAGASSIATALNFLVTIITMRAPGMNLWRMPLLVWAN
                     LSTSLLVVGATPFVAGAQFMVFFDRILGMNFFNFLEGGDVISYQHIFWFYSHPAVYIM
                     MLPGFGIISEIISTHARKPVFGYRLMALALIGIVVLSYSVWAHHMFVAGMFSWLRVPI
                     MITTMLIAVPTGIKVFSWLGTLFYGKIHTYSTAMLFSLAFIITFTVGGISGVMTAMIP
                     LDIHVSDTYFIVAHIHFVLFGGSVFTIFAGIYHWFPKMTGRMYDERLGRIHFWGTLVG
                     TWMTFVPMHWIGMDGMPRRVADYATQYGNWNLLISVSAFVLGAVQLIFLYNMIVSWRF
                     GPKAGNNPWRANSIEWQVSSPPPVFNFDEIPRVVGGPYEYGTPGARHAIMGGEGEGAE
                     VPETQPSTVTAPS"
BASE COUNT          805 a         1205 c         1436 g          666 t
ORIGIN      
        1 ggcagaaccg ccgaacgcgc ccgcgaactc ggcctcgacc tcgagtccgc actggcctca
       61 cacgccgcct cggagccgct gagggccctt gaggacctga ttgacaccgg acccaccggc
      121 accaacgtca acgacctctt cgtcgcggtc acgggcccga tctgaaccag ccgggctgac
      181 ggagcggcgc tactcgtcgt cgtcggggct tccgaggccg tgcctgaggc cgcggaagat
      241 cggcacgcta acgatgccca ggaggatcag cacgaggatc accgcggcga ggggcgagac
      301 cagcttgccg accgctccca ccgctaccgc ggcgatgatg accacgatca gccagctgaa
      361 tgcggcactc tcccgctcga ccggattgcc gagatcgaaa ccgaaccgct caccccgggc
      421 gggcaccggc gactgctcca cctcggtgga ttcgtcccct tccccggcct cgggctcgct
      481 catggccggg tcagatgatg tagacggcga ggaacacgat gatccacatg atgtcgacga
      541 agtgccagta gatgcctggc acctcgacac cgaggtggtc cttctgttcc ggaccgaagt
      601 ggccgcggta ggcgcgaatg ttggcgaagg tcaagagcag caggccaacg aagacatggg
      661 cgccgtggag cccggtgagg ctgaagaaga tcgaaccgaa ggcaccgtca cgcggcgtga
      721 agccgatgtg gatgtactcg ttgatctgga tgaagaggaa ggtcgcgccg aggagccagg
      781 tcgtggtcag gccgagcttc agaccgcgat ggttgttgtg cttgatcgcc tcgagcgcgt
      841 aatggaccgt gaacgaggag gaaaccagga tgcaggtgtt gatgccggcg atcaggaccg
      901 ggagctcgac gccctccggc ggccaggaag ccggatcggc gacgacccgc aggaagaagt
      961 aggcggcgaa gaaggcgccg aacagcatca cctcggaaag gatgaaaagc aggatgccga
     1021 ggatcgtgcg gtcgatgcgg gcgctctgat gcgcctcggg cgggccgtga tggtggtcgt
     1081 cgacgtgacc gtgggcggcg atgcttgcgc tttccatctc tatgcctcgt tctcttccgc
     1141 ggttgaatct tcagcgccag cggcttcggg agcctcgccc tcggcgacca cggcagcgac
     1201 ggcctccggc gggcggccgg cctcgtggtg gaccacgggc ttctcggtga tctcgcgcac
     1261 ccggccgatc aggtcttcct tcagccagtg ggactcttca ccgctcaggg tcgaaatcag
     1321 gatctcgtcg acccggtaat gctcgatcgc gttcttcacg gcgtcgaacg ggtccgggct
     1381 catcggctgg ccggtggcgt cgatgtcggc gtggtagagc ttggccatga cggtcgccag
     1441 gttccgggca acggtggcct cggaaacatc gtcggcggcc ggagcgatga tcgtgtaccg
     1501 gtggggacgg tcgccggagc ggtcgagcag acgctcctga aggtcctcgg aggtcagggt
     1561 ccggttggcg acgaccaggg tgtgggttac gttcaggctg atcgagtcgt cctcgatgcg
     1621 aaccggaatg tgggtgatgt tggtgacgtc gccgtaggtg atctcggccc aggagagcag
     1681 gtcgcgcctg gtcaggccga aacggaagtc ataggtgcag gagagcagaa cctcgccggg
     1741 gcgatggctg cgaatcgcgt cgccgagggc caggtcgtac tcggggtcga gcacttcgcc
     1801 gattgcctcg ataccaaact cgtcgagcac cgcgagggtc acgtcaaccc gggcccgggc
     1861 ggcttccgcc ctttcctcgg gatcgaagag ctgaccgacc gaaggctggt tctgcggggc
     1921 gacgaccacc acgtaggagg cgtcggccgc cttttcgcgg gccgcctcga gcagcttgcg
     1981 gccgccggcg acctcgttca ggaaaatcag gaggggcttg ctcatgacgg cgcagtcacc
     2041 gtgctgggct gggtttcggg cacttccgcc ccttcgcctt cgccgcccat gatcgcgtga
     2101 cgcgcacccg gcgtgccgta ctcgtaggga ccgccgacga cccgcgggat ctcgtcgaag
     2161 ttgaagaccg gcggcggaga ggagacctgc cactcgatcg agttcgcgcg ccacgggttg
     2221 ttgcccgcct tcgggccgaa ccgccagctg acgatcatgt tgtagaggaa gatcagctgg
     2281 accgcaccga gcacgaaggc cgagacggaa atcagcaggt tccagttgcc gtactgcgtc
     2341 gcgtaatcgg cgacacggcg gggcatgcca tccatcccga tccagtgcat cgggacgaag
     2401 gtcatccagg taccgaccag ggtgccccag aagtggatcc ggccgagtcg ctcgtcatac
     2461 atgcgtccgg tcatcttcgg gaaccagtga tagatgccgg cgaagatggt gaagaccgag
     2521 ccgccgaaga gcacaaaatg gatgtgggcg acgatgaagt aggtgtccga gacgtggatg
     2581 tcgagcggga tcatggcggt catcacgccc gagattccgc cgacggtgaa ggtgatgatg
     2641 aaggccagcg agaacagcat cgccgtcgaa taggtgtgga tcttcccgta gaagagcgtg
     2701 cccagccagg agaagacctt gatgccggtc ggtaccgcga tcaacatcgt ggtgatcatg
     2761 atcggcactc gcagccagct gaacatgccg gcgacgaaca tgtggtgcgc ccagaccgag
     2821 taggagagca cgacgatgcc gatcagggcg agggccatga gccggtagcc gaagaccggc
     2881 tttcgggcat gggtggaaat gatctcggaa atgatcccga agccgggcaa catcatgatg
     2941 tagacggctg ggtgtgagta gaaccagaag atgtgctggt acgagatgac gtcgccgccc
     3001 tcgaggaagt tgaagaagtt catcccgagg atgcggtcga agaagaccat gaactgggca
     3061 ccggcgacga acggggtcgc accgacgacc agcagcgagg tcgagaggtt ggcccagacc
     3121 agcagcggca tgcgccagag gttcatgcca ggcgcccgca tggtgatgat cgtgaccagg
     3181 aagttgagtg ccgtggcgat cgaggaggca cccgcgaact gcacgccgat gttgaagaag
     3241 gactggccta acggggctcc ggtcgagagc ggcgcgtaac cggtccagcc ggcgtcgaag
     3301 gaaccgcccg gggcgaggaa actgccgagg aagatgatgc cggcgatcgg cagcagccag
     3361 aaggaaagcg cgttgaggcg cgggaaggcc atgtccgggg cgccgatcat cagcggcagc
     3421 acgtagttgg cgagcccggc gaagatcgga atgatgaaga ggaagatcat caccaccgcg
     3481 tgggtggaga agagaccgtt gaaggtgttg gcggcgacga tctggccgcc cggctgggcc
     3541 agttcggccc gcatcagcag ggcgagcagg ccaccgatca ggaagaagaa cagggtggtc
     3601 accacgtact gggtgccgat taccttgtgg tcggtgttga aggtgaagta gtcctgccag
     3661 cgggtggcgc cgtggccgga atgatcttcc gggatggtcg gcttgcctgc cgcccagcgg
     3721 aaccagtagt caaaggcacc gaagccgaag aggaaaccga agggcgtggt gatcagcggg
     3781 acgatgacct gcgggtagcc ggtctgctcg gtctggaagg cgtcaaggcc cgaaagaagg
     3841 cgcagtccga ccaccagaaa gaacccgaac gcggcaccga gaacggttcc gatcgctgcg
     3901 cgataccaac cgggttgcct gattgccgct gccatttact tgacctccat catcgggttg
     3961 tggaaccggg ccttcactgt tctttgaccc cgcttccttc aatgcccccg gactgctcct
     4021 gctgctgttt ctgcttctcc agcgcctgat cggtggtcag gagaagatcg tcaggcactt
     4081 cggtggtctg ctgcttgagc caggcctggt ac
//
LOCUS       DAOQMF010000060         4112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_10758_length_4112_cov_3.615479, whole genome shotgun sequence.
ACCESSION   DAOQMF010000060 DAOQMF010000000
VERSION     DAOQMF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10758_length_4112_cov_3.615479"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..576)
                     /locus_tag="PLV77_01660"
     CDS             complement(97..576)
                     /locus_tag="PLV77_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HNC14584.1"
                     /translation="MSRRAEITAAARSILEQEGEEALTMRNLADRLGIKAPSLYKHVS
                     SRREIETLLAAEALNEMRESLAAADGLEEMGRSYRAWAIANPSLYRIATTRPLDRENL
                     PTGLEDAAAAPLLAAVDGDRARARAFWALAHGLVLLELDGRFPGDADLEAAWLAGIS"
     gene            complement(573..1049)
                     /locus_tag="PLV77_01665"
     CDS             complement(573..1049)
                     /locus_tag="PLV77_01665"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNC14585.1"
                     /translation="MPSIHTTELMDAPPDRVWQILADLSSYPDWNPFIIEASGELREG
                     EKLLLRMQPPGGKAMNFRPTVLKADPGQELRWLGRLGFRGIFDGEHYFRLTPEGGGTR
                     LDQGEEFTGLLPRFMGKVLSQTERGFEALNAALRKRAERDVEAPAGLAPEKETAGS"
     gene            complement(1118..1924)
                     /locus_tag="PLV77_01670"
     CDS             complement(1118..1924)
                     /locus_tag="PLV77_01670"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNC14586.1"
                     /translation="MSEVLPGPGSNIALRTLPNLRDLGGYATGDGRVVRRGQLYRSVL
                     LGRLSDEDVARVRELDLQVIFDFRTAAEHRATPDRDVGARTINLDVLADRTGEGPASL
                     LSKMDDPEAIKAALTDGRGAGLMRTAYRELIELPSATRSYSAFFTEVATADSLPALFH
                     CTTGKDRTGWAAAATLLFLGVDEEDVYRDYLLTNEQLLPALKPILSHIEEAGIEFDLL
                     RPVIGVDRSYLETAVDLMKERYGSIDGYMRDGLGLEDGTLLALRRRLLGT"
     gene            complement(1921..3384)
                     /locus_tag="PLV77_01675"
     CDS             complement(1921..3384)
                     /locus_tag="PLV77_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HNC14587.1"
                     /translation="MSNGQDRTPYVDALLAFAQSDPGRFNVPGHQGGIGADPLLHQLV
                     GQTGLSDDIPGLIEGIDVGDPNPFQEAQALAAGAWGAKRTWFLINGASQGNQAMCLAV
                     AHMGGELVVQRNVHSSVIDGMIMAGLKPTFAAPELDPDLGVAHCLTPDDLAEALDRCP
                     DASAAVVVSPTYFGACADISGLAEVAHSRGVPLVADEAWGAHLHFHPDLPPSAIESGA
                     DLVISSTHKIVGSLTQSAMIHLATDRFDQAIIDRCISMTESTSPNSLLCGSLDAARRQ
                     AAIHGERLLGETIEVLVEAREEIRSIPGLDVLDERLLERKSVVGWDPLRLTIDVRGTG
                     STGYHIAELARDLAGVNLELASDTVAVAVFGMGTGTAQRARRLIDGVLTALEAIEAEP
                     VQEKPEFAPPPLWGDPAMTPRDAFLGKQEVVPFSEAEGRIAAEPLATYPPGIPNVLPG
                     ERLTRETLDYITDSVDHGGYVRGAADRELTTLRVIAE"
     gene            3436..4005
                     /locus_tag="PLV77_01680"
     CDS             3436..4005
                     /locus_tag="PLV77_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14588.1"
                     /translation="MNLPSDTTRASRFIPAAFLCALSIALLAGCGGGSSEEDLSREGF
                     IADADAICAQYQPTSATLEQQFNQAIGNGELEDAAQDFEDQAADFSAMLDELEGLTAP
                     TADQTTVTQIIDLGRQRVDIAKEAADAIASGDKDAMISAGKKASILAGQYYQLTDGFG
                     FETCGSSGASGTTGTTGPAGSTGTTGATS"
BASE COUNT          663 a         1368 c         1406 g          675 t
ORIGIN      
        1 ctacacggtc agcgagggcg ggtcgaggct ctgcttcgta aaagaccccg acggctaccg
       61 gatcgaactg atcgaaggcg acccgggtcg cgccgactag gaaatccccg ccagccaggc
      121 ggcttcgagg tcggcgtccc cggggaaccg gccgtccagt tcgagcagca ccaggccgtg
      181 cgccagggcc cagaaggcgc gggcccgtgc ccggtcgccg tcgactgccg cgagcagagg
      241 tgccgcggcg gcgtcctcga gtccggtcgg cagattctcg cggtcaagtg gccgggtggt
      301 ggcgatccgg tagagcgacg ggttggcgat ggcccaggcc cggtaggaac ggcccatctc
      361 ttccaggccg tcggctgcgg cgagtgattc acgcatctcg ttgagggcct cggcggccag
      421 cagcgtttca atctcccgac ggctgctcac gtgcttgtag agcgagggag ccttgatgcc
      481 gagacggtcg gcgaggttgc gcatggtcag ggcctcctcg ccttcctgct cgaggatcga
      541 ccgggccgcg gcggtgatct ctgcgcgccg gctcaactgc cggccgtttc tttctccggc
      601 gcaaggccgg ccggcgcttc gacgtcgcgt tcggcccgct tcctgagcgc cgcgttcaaa
      661 gcttcgaatc cccgctcggt ctggctgaga accttgccca tgaagcgggg gaggaggccg
      721 gtgaattcct caccctgatc aagcctggtc ccaccgccct ccggggtcag gcgaaagtag
      781 tgctcgccgt cgaagatgcc ccggaacccg agccggccca gccagcgcag ctcctggccg
      841 ggatcggcct tgagcaccgt cggcctgaag ttcatcgcct tgcccccggg aggctgcatc
      901 ctgagcaaga gcttctcgcc ttcccggagt tcgcccgagg cttcgatgat gaacgggttc
      961 cagtccgggt aggaggaaag gtcggccagg atctgccaga cgcggtcggg cggggcatcc
     1021 atcaattcgg ttgtgtggat cgaaggcatg ggtcgaggct aacacagtta gccaacgatg
     1081 gcaaacactg ttagccgtac tacccccgaa gcaggcctca ggtaccgagc aggcggcgcc
     1141 gcagggcgag cagagtgcca tcttcgaggc cgagcccgtc gcgcatgtag ccgtcgatcg
     1201 atccgtagcg ctccttcatc aagtcgaccg cggtctccag gtacgagcgg tcgaccccga
     1261 tcactggccg gagcaggtcg aactcgatcc cggcttcctc gatgtggctg aggatcggct
     1321 tgagggcggg cagcagctgc tcgttggtca gcaggtaatc gcggtagacg tcctcttcgt
     1381 cgaccccgag gaagagcagg gtggcggccg ccgcccagcc ggtgcggtcc ttgccggtgg
     1441 tgcagtgaaa gagggccggc agcgaatcgg cggtcgcgac ttcggtgaag aaagcgctgt
     1501 aggaccttgt tgccgaggga agttcgatca gttcccggta ggcggtgcgc atcaggccgg
     1561 cgccacgccc gtcggtcagc gccgccttga ttgcctccgg gtcatccatc ttcgagagca
     1621 gactggccgg cccttcgccg gtccggtcgg cgagcacgtc gaggttgatc gtcctggcgc
     1681 cgacgtcccg gtccggcgtc gcccggtgct cggccgcggt ccggaaatcg aagatcacct
     1741 gcaggtccag ctcgcggacc cgcgccacgt cttcgtcact gagccggccc aacagcaccg
     1801 accggtaaag ctgcccccgc ctgacgaccc gcccatcgcc agtcgcgtaa ccgccgaggt
     1861 cgcgcaggtt gggcagggtc cgcagcgcga tgttcgaacc cggcccgggc aggacctcgc
     1921 tcattcagcg ataacccgga gcgtggtcag ctcgcggtcc gccgcacctc gcacgtagcc
     1981 cccgtggtcg accgagtcag tgatgtagtc gagggtctcg cgggtcagcc gctcgccggg
     2041 caggacgttg gggatcccgg gcggataggt ggcaagcggt tccgcggcga tccggccctc
     2101 tgcttccgag aagggcacga cttcctgctt gccgaggaac gcgtcccggg gagtcatcgc
     2161 gggatcgccc cagagcgggg gcggcgcgaa ctccggcttc tcctggaccg gttcggcctc
     2221 gatcgcctcg agggcggtga ggacaccgtc gatcaggcgg cgggcccgct gggcggtgcc
     2281 cgttcccatg ccgaatacgg cgacggcgac cgtgtccgag gccagttcga gattgactcc
     2341 ggccaggtcc cgggccagct cggcgatgtg gtacccggtg cttccggttc cccggacgtc
     2401 gatggtcagc cggagcggat cccagccgac cacgctcttg cgctcaagca gcctttcgtc
     2461 gagcacgtcg aggccgggga tcgagcggat ctcttcccgc gcctcgacca gcacttcgat
     2521 cgtctccccg agcaaccgct ccccatgtat ggcggcctgc cgtcgggcgg catcgagcga
     2581 accgcaaagc agcgagttcg ggctggttga ctcggtcatc gaaatgcagc ggtcgatgat
     2641 cgcctggtca aaacggtcgg tggcgaggtg gatcatcgcc gactgggtca ggctgccaac
     2701 gatcttgtgg gtggaggaaa tgaccaggtc ggcgccgctc tcgatcgcgc tgggcggcag
     2761 gtcagggtga aagtggaggt gggcccccca ggcttcgtcg gcaaccaggg gaacgccgcg
     2821 gctgtgggcg acctccgcca gtccggagat gtcggcgcag gccccgaagt aggtgggtga
     2881 gacgaccacc gccgcactcg catccgggca gcggtccagc gcctcggcca ggtcgtcggg
     2941 agtcaggcaa tgcgcgacgc cgaggtccgg gtcaagctcc ggggcggcga aggttggctt
     3001 caacccggcc atgatcatgc cgtcgatgac cgaggagtgg acgttccgct ggaccaccag
     3061 ctccccgccc atgtgggcga ccgccaggca catcgcctgg ttgccctgcg aggccccgtt
     3121 gatcagaaac caggtccgct tcgcgcccca tgctccggcg gccagcgcct gggcctcctg
     3181 gaacggattc ggatcaccaa cgtcgatgcc ctcgatcagt ccggggatgt catcggaaag
     3241 gccggtttgc ccgaccagct ggtgcagcag gggatccgca ccgatcccgc cctgatgacc
     3301 tggcacgttg aacctgcccg gatcactctg ggcaaaggcg agcagcgcgt cgacataagg
     3361 agttctgtcc tgaccgttag acatggcaac gagtctattg acggccgcgc cgggtccgga
     3421 gataggctgc cgatggtgaa tctcccctct gacacaaccc gcgcttcgcg gttcatcccg
     3481 gccgcgtttc tctgcgctct ttcgatcgcc ttgctggccg gctgtggcgg gggctccagt
     3541 gaagaagacc tctcgcggga gggcttcatc gctgacgctg acgcgatctg cgcccagtac
     3601 cagccaactt cggccacact cgagcaacag ttcaaccagg cgatcgggaa cggcgagctc
     3661 gaggatgccg cccaggactt tgaggaccag gccgcggatt tctccgccat gctcgatgaa
     3721 ctcgagggcc tgaccgcccc gactgccgat cagaccacgg tgacccagat catcgacctc
     3781 ggccggcagc gcgtcgacat cgcgaaggag gcagccgatg cgattgcctc gggtgacaag
     3841 gacgcgatga tctccgcggg caagaaggct tcgattctgg ccggccagta ctaccagctg
     3901 accgacggct tcggcttcga aacctgtggc agctccgggg cctcgggcac gaccggcacc
     3961 accggaccgg cgggcagcac cggcaccacc ggcgccactt cctgaaccgg cagggaagcg
     4021 gccccgcccg ggatcacccg taggggtacc accagtcctt gacttcgatc tcggtgtcga
     4081 tgtgggcatg cttggcttcg cggaaggcgt ct
//
LOCUS       DAOQMF010000061         4061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_11001_length_4061_cov_3.533699, whole genome shotgun sequence.
ACCESSION   DAOQMF010000061 DAOQMF010000000
VERSION     DAOQMF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11001_length_4061_cov_3.533699"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLV77_01685"
     CDS             <1..678
                     /locus_tag="PLV77_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNC14589.1"
                     /translation="GSTLEAWVKPDDAGDMAILEHGGAGALFIRGGKVVFRHVGSEIE
                     SPLGAAPVGQTTQVVGVWDGVNMILYVNGVEVASKEAPGQPSGSSTLYLGLANVGLAS
                     PWLRGSLDEVSVYSHALEAGQVDQHFRADPPPPAEDNPSSHDPDQPAMDDHVVDDPDQ
                     PVGDDPDQSSTDDPVGSVEAESGGSSPKSGVKSNSKARRSALKKCQRMSNRSKRNRCL
                     RHVRNRF"
     gene            755..1036
                     /locus_tag="PLV77_01690"
     CDS             755..1036
                     /locus_tag="PLV77_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14590.1"
                     /translation="MKLYVCWGTFQVPGAREHPCKIAYDALVQADFEPEVIKAHSVGF
                     LPRAVQTGARKKVEDATGSKWVPALETDEGEWISGSREIVAWAEQHARV"
     gene            complement(1040..1387)
                     /locus_tag="PLV77_01695"
     CDS             complement(1040..1387)
                     /locus_tag="PLV77_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14591.1"
                     /translation="MSAVVLGLLFIFILIMVTRPALLVGVSADDLAHSLANTTSPSGG
                     SNCRKEADGNWTCNFAPPDGSWRRYEVSVDWLGCWDATLPGADTSTSGTSSSPYDKSG
                     CIELGDVIRIESD"
     gene            complement(1427..2989)
                     /locus_tag="PLV77_01700"
     CDS             complement(1427..2989)
                     /locus_tag="PLV77_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14592.1"
                     /translation="MTTSHRLRSWGPPLAFIAVISAVLLAIFPKGFPNYDTIYYLLWG
                     REIADGLSPDYGAPLAPTPHPLYDLLGALFSPLGDGSITVAVVLAYVSLGLLAWLVYR
                     LGSEWFDRWIGVLAAFLVMTSAPVLSNGLRAYIDIPYMVLCLAALLIETKKPKAGWPV
                     LVLLALAGLLRPEAWLFSGFYFLWLALDFHVSPAEGADTSAPSRRAGISLGRLRIQLR
                     ESGIDRNLILMACLTAAGPVIWVLFDLITTSNPLYSFTGTRETVETLERDTGPLDVII
                     YGPRRLGEVMQWPGMVGAFFGVILTLWAMPKRAAIGVISAFLALVAFALMGASGLAII
                     PRYTMLAAAILFIFTAAAVLGWRLLEPGHRLRRIWQVAAAITVALWLIWLPNQYDLLS
                     TVDTDLSDQALVERDLEALVDDGAFNPPSDLPGETSVEDCLPISVPNHRAVPRLAFWL
                     DIRPSDVVSIAAADEQPDSGFFLAPANEFTVENFILDPGEPGRAVTTPPPGFEEVARN
                     RSWVLYTSCLAG"
     gene            3073..>4061
                     /gene="dnaX"
                     /locus_tag="PLV77_01705"
     CDS             3073..>4061
                     /gene="dnaX"
                     /locus_tag="PLV77_01705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNC14593.1"
                     /translation="MSESTSLYRRHRPHSFDQVVGQQHVVRTLSNAITQDKVHHAYLF
                     VGSRGTGKTSMAKILAASLNCENGGPTLTPCGTCESCRSIAAGNSVDVIEMDAASNRS
                     VDDIRELRDRVAFAPTAGRWKVYILDEAHMLTKEAWNAFLKTLEEPPPNTVFILATTE
                     AHKVMPTIADRCQRFDFQRPSIEQLSEVLTRVAAAEQITIGAPAISMISRSASGSFRD
                     ALGTLDQLVSYSGNEIALDDVLEVLGAADADLLFGVVDKVIEADPAGVLTAVEQMARS
                     GRDPARFARDLLGHLRVLLVPQTTGQIPEAFAVTAADPDRLVRQAKAIGPANL"
BASE COUNT          788 a         1303 c         1327 g          643 t
ORIGIN      
        1 ggctccacac tcgaggcctg ggtcaagccg gacgatgccg gggacatggc gattctcgaa
       61 catggcggcg ccggggcact gtttatccga ggcggcaagg tggttttccg tcacgtagga
      121 agcgagatcg agtcgcctct cggtgcggca ccagtcgggc aaacgactca ggtggtcgga
      181 gtctgggacg gcgtcaacat gatcctttac gtcaacgggg tggaagtcgc ctctaaggaa
      241 gcccccggtc agccttccgg ctcctcgact ctctatctgg gattggccaa cgtcgggctc
      301 gcctcaccgt ggctgcgcgg ctcactcgac gaggtctctg tttacagcca tgcccttgaa
      361 gctggtcagg tcgaccagca cttcagggca gatcctccac ccccagcgga ggacaacccg
      421 tcgagccacg accccgacca acctgccatg gatgaccacg tagtggacga ccccgaccaa
      481 ccggtcggcg acgaccctga ccagtcgtcc acagatgacc cggtcggtag cgtggaagcc
      541 gagagcggcg gatcgtcacc gaagtccggc gtcaagtcga actccaaggc cagaaggtcc
      601 gcattgaaga agtgccaacg aatgagcaac cgctcgaagc gcaaccgctg cctgcgccac
      661 gttcgtaacc ggttctagag ccacgctaaa ccgcactttt tccacaaatg atgtgggaaa
      721 acgacagctt cacggcgccg attagtctgg tcccatgaaa ctctacgtct gctggggaac
      781 ttttcaggtg ccgggagcgc gggagcatcc ctgcaagatc gcctatgacg cactggttca
      841 ggctgacttc gagccggagg tcatcaaggc ccacagcgtc ggtttcctgc cccgcgccgt
      901 tcagaccggc gcccggaaaa aggtagagga cgccacggga agcaagtggg tgccggcgtt
      961 ggaaacagac gagggagaat ggatctcggg atccagagag atagtcgcct gggccgagca
     1021 gcacgcccgg gtctgacctt cagtcgctct cgatcctgat cacgtcgccc agttcgatgc
     1081 agccggactt gtcgtaaggg gagctggagg ttccggaggt gcttgtgtcg gcgcccggca
     1141 aggtggcgtc ccagcagccc agccagtcga cgctcacctc gtaacgtctc caggatccat
     1201 ccggaggggc gaagttgcag gtccagtttc catcagcttc cttgcggcaa ttactgccgc
     1261 cgctcggcga ggtggtgttg gccagcgagt gggccaggtc atcagctgaa acgccgacca
     1321 gaagtgcagg ccgagtgacc atgatcagaa tgaagatgaa caggagcccc aggacgacgg
     1381 cgctcaaggc cccgaggatc tttacgccct tccaccacac ggtggctcag cccgccaggc
     1441 agctggtgta gagcacccag gaccggttgc gggcgacctc ctcgaaaccg gggggaggcg
     1501 tggtgacggc acggcccggc tcgcccgggt cgaggatgaa gttttcgacc gtgaactcgt
     1561 tggccggggc gaggaagaag ccggaatctg gttgttcgtc cgcggcggcg atcgacacga
     1621 catcagatgg ccgaatgtcc agccagaagg cgagacgggg cacggcccga tggttcggga
     1681 ccgagatcgg caggcagtcc tcaaccgagg tctccccagg gaggtcgctc gggggattga
     1741 aggcaccgtc atcgacaagg gcctcgaggt ccctttcgac cagggcctga tcgctcaggt
     1801 cggtgtcgac ggtgctgagc aggtcgtact ggttgggcag ccagatcagc cagagcgcga
     1861 ccgtgattgc ggctgccacc tgccagattc gccgcagcct gtgaccgggc tcgagcagcc
     1921 gccagcctag taccgcggca gccgtgaata tgaacaggat cgccgcggcc agcatcgtgt
     1981 agcgggggat gatcgccagg cccgaagcac ccatcagggc gaaggcgacc agggccagga
     2041 aagccgagat caccccgata gcggcccgct tcggcatcgc ccaaagggtc aggattactc
     2101 cgaagaaggc acctaccatg cccggccact gcatcacttc gccaagccgg cgtggtccgt
     2161 agatgatcac gtcgagtggt ccggtgtctc gctccagcgt ctcgaccgtc tcgcgggtgc
     2221 cggtgaagga gtagaggggg ttcgaggttg tgatcaggtc gaagagaacc cagatcaccg
     2281 gtccggccgc cgtcaggcag gccatcagga tcaggttcct gtcaatccct gactccctca
     2341 gctggattct caggcgtccg aggctgatcc cggcgcgtcg agacggagct gaggtgtcgg
     2401 cgccctccgc aggcgacacg tggaaatcga gggcgagcca gaggaagtag aagccggaga
     2461 agagccaggc ctccggtctg agcaggccgg ccagggcaag caacacgagt acgggccagc
     2521 cagccttcgg cttcttcgtt tcgatcagca gggcggcaag gcagagcacc atgtacggga
     2581 tgtcgatgta tgcccggaga ccattgctca ggaccggtgc gctcgtcatc accagaaacg
     2641 cggcgaggac cccgatccag cgatcgaacc attccgagcc cagccggtag accagccagg
     2701 ccagcaggcc gaggcttacg taggcgagga cgaccgcgac ggtgatcgag ccgtcgccca
     2761 gcggtgagaa aagcgccccg agaaggtcgt agagcgggtg cggggttggg gcgagcgggg
     2821 cgccgtaatc cgggctgagg ccatcggcga tctcccggcc ccagagcagg taatagatcg
     2881 tgtcgtagtt cgggaagccc ttgggaaaga tcgccagcag gaccgccgag atgacggcga
     2941 tgaaggcaag cggcggcccc caggaccgga gtcggtgaga cgtcgtcatc gggccgtaaa
     3001 gcctgtcaca tgaggcgggt acactgggct ggtgccgccc cgggagactg ttccgttgcg
     3061 gcggaccagc tcatgagcga atcgacctcc ctctaccggc gacaccggcc ccactccttc
     3121 gaccaggtgg tcggacagca acatgtggtc cggaccctgt ccaatgcgat cacccaggac
     3181 aaggtccatc acgcctacct gttcgtcggc tcacggggta ccggcaagac atcgatggcg
     3241 aagatcctcg ccgcctcgct gaactgcgag aacggcggtc cgaccctcac cccctgcggc
     3301 acctgcgaat cctgccgctc gatcgcggcc ggcaattcgg tcgatgtgat cgagatggat
     3361 gcagcctcca accgctcggt cgacgacatc cgcgaactgc gggaccgggt cgccttcgcc
     3421 ccgaccgccg gccgctggaa ggtctacatc ctcgacgagg cgcacatgct gaccaaggag
     3481 gcctggaacg ccttcctcaa gactctcgag gaaccgccgc cgaacacggt cttcatcctc
     3541 gccacgaccg aggcccacaa ggtgatgccg accatcgccg accgttgcca gcgcttcgat
     3601 ttccagcgac cctcgatcga acagctctcc gaggtgttga cccgcgtcgc ggccgccgag
     3661 cagatcacga tcggcgctcc ggcgatctca atgatcagcc gctccgcttc gggaagtttc
     3721 cgcgacgcgc tcggcactct ggaccagctc gtctcctaca gcgggaacga gatcgcgctc
     3781 gacgacgtgc tcgaggtgct gggggcggcc gacgccgacc tgctcttcgg tgtggttgac
     3841 aaggtgatcg aggccgaccc ggccggcgtc ctgaccgcgg tcgagcagat ggcccggtcc
     3901 ggtcgcgacc cggcccgctt tgcccgcgac ctcctgggcc accttcgggt gcttctggtc
     3961 ccccagacaa ccggccagat ccccgaggca ttcgcggtga ctgccgccga cccggaccgt
     4021 ctggtccgcc aggcgaaagc gatcggacca gccaacctga t
//
LOCUS       DAOQMF010000062         4037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_11097_length_4037_cov_3.699146, whole genome shotgun sequence.
ACCESSION   DAOQMF010000062 DAOQMF010000000
VERSION     DAOQMF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11097_length_4037_cov_3.699146"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLV77_01710"
     CDS             <1..788
                     /locus_tag="PLV77_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNC14594.1"
                     /translation="DLQALLSTLGTLGVQLPGGSGIIDTILGQVTDLTCGFSPMDVTF
                     TTGETPQGSGAPFSEGPLGPGALTAEWSQLGPFAGKTKILGLIDACQAIKMYAPTLLE
                     SALGSSGIDLASLGLGDIDLASLLDNLDNLNLGPSSLTLTRTLDESPDPPAAEPVLKM
                     SVKGPNRKVKAGSKAEFTVKVKNSGQGDATGVRVCLKSSGPVTVQGSRCRTLGKVMAG
                     ATQTRHFKVKAKKGKKKSAKISFSLKSSNGDDASASTRLLIRH"
     gene            complement(730..969)
                     /locus_tag="PLV77_01715"
     CDS             complement(730..969)
                     /locus_tag="PLV77_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14595.1"
                     /translation="MIARQARHALAADVHLRPALLELGATCPTPSLVALESELASLGA
                     DGLVTAWLERSGNWLQLSDGSAAGCSPRRHRHLTT"
     gene            complement(969..1301)
                     /locus_tag="PLV77_01720"
     CDS             complement(969..1301)
                     /locus_tag="PLV77_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNC14596.1"
                     /translation="MNQNSLHPGFTPNVVAIVGNQRPDSRTLRLASGIADGLAAQLGD
                     TPAVAIDLVNLPLENGGFSPGLNDLIRDSRVVIAASPTVMGSYSGLLKIFLDGLPEAA
                     LLDKTGIP"
     gene            1524..2441
                     /locus_tag="PLV77_01725"
     CDS             1524..2441
                     /locus_tag="PLV77_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14597.1"
                     /translation="MVGEPPRPKQPPGRIGTLARLFHPKALNRKHPHVSDRLRTFMAT
                     LTFLELVLIFVLLIVASTVLLGLTFWWLAPSQLYNSYWDALGSAFFTLIGMPPGYLDP
                     AELSGRFKVAVGAAGLFSLLLPALFLGAIVFRLFVRLRIFVLKERPTLTRKKVRGTEG
                     WYLAIRLYSSTAILIVDIDFKVYLRMKSSNGLLLSNEEIRVTNATWPIADTHVPYTLY
                     LPVDDGDLDANEHLISLQGHDLETCESAIIHIRGASPQLASEVNETHTLKVPLDLSPA
                     DFGGVQVLYDGRGPDWVGWDEFEADRPLQ"
     gene            2438..3007
                     /locus_tag="PLV77_01730"
     CDS             2438..3007
                     /locus_tag="PLV77_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNC14598.1"
                     /translation="MTDWVFGYGSLVTDLVTSSDENGFEWFWANLGNHRRDWGAGMEN
                     AAADADWKYYVDSRGTRPDIVVAFLDAYPHSGSSINGVVFAVDESRLREIDRREVRYS
                     RVDVTDLVTTAGWGDGRVWTYRGSEKSREVALRGRREDRAFVSRDYAAHCRLGFSVGG
                     EQALHQFQLSTDPLDLPLRELTMFLSDPG"
     gene            3072..>4037
                     /locus_tag="PLV77_01735"
     CDS             3072..>4037
                     /locus_tag="PLV77_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC14599.1"
                     /translation="MKSSVPFRAAVAAVVLLAAVALAIDGCGKDEETETTRTLRIYVS
                     QPLKGDRGGEDMLRAVEIANEDEGGTIGPVRIEIVGMNDSDESGNWVASLVRRNARRA
                     AADDSAVAYIGDFDSGATEVGMPILNRAGMLQVSSSATAVKLTKPLPELGEQIRPTGI
                     RTFGRVVPNDNVQAAALALFMDQESVNEVFVVDDGDTYGEGLRFRFEKVARHTGIEIT
                     GGATVTRSIQIGDVAKRVIGSGADALLFAGSNLDLARDLFETVHREDNFIKLFGGDGL
                     SLSSFLGSLGDTGLDTYITAPQLPAGNYARSGESFLRGFQERYGRP"
BASE COUNT          732 a         1270 c         1271 g          764 t
ORIGIN      
        1 tcgacctcca ggccctgctc tccacgctcg gcacgctggg agtccagctg ccggggggtt
       61 cgggaatcat cgacacgatc cttggccagg tgaccgacct gacctgtggg ttctcgccga
      121 tggacgtcac cttcaccacc ggtgagaccc cgcagggttc cggcgccccg ttcagcgagg
      181 ggccgctcgg gcccggtgcg ctgaccgccg agtggtcgca gctcgggccc ttcgcaggca
      241 agaccaagat cctcggtctg atcgacgcct gccaggcgat caagatgtac gcgcccacgc
      301 tgctcgagag tgcccttggc agctccggca tcgatctcgc cagccttggg ctcggtgaca
      361 tcgacctggc ttccctgctc gacaacctcg acaacctgaa tctcggcccc agctctctca
      421 ccctgacccg gacactcgac gagagcccgg acccgcccgc ggcggagccg gtgctgaaga
      481 tgagcgtcaa gggaccgaac cgcaaggtca aggccggcag caaggcggag ttcaccgtca
      541 aggtcaaaaa ctcgggtcaa ggtgacgcga ccggcgtccg ggtctgcctg aagtcttccg
      601 ggccggtgac cgtccaggga agccgctgcc ggaccctcgg caaggtgatg gccggtgcca
      661 cccagacccg tcatttcaag gtgaaggcga agaagggcaa gaagaagtcc gccaagatca
      721 gcttctcgct caagtcgtca aatggcgatg acgcctcggc gagcacccgg ctgctgatcc
      781 gtcactgagc tgcagccagt ttccgctccg ttccagccag gcggtgacca gaccgtcggc
      841 tcccagtgag gcgagctccg actcgagcgc gaccagcgac ggggtcgggc aggtcgcgcc
      901 aagttccagc agggccggtc gcaggtggac gtcggccgcc agggcgtgac gggcctggcg
      961 cgcgatcatc aggggatccc ggtcttgtcg agcagcgccg cctcgggcag gccgtcgaga
     1021 aagatcttga gcaggcccga gtagctgccc atcacggtcg ggctggccgc gatcacgacc
     1081 cgggagtccc ggatcaggtc gttcagtccc gggctgaagc cgccgttttc cagcggcagg
     1141 ttgaccaggt cgatcgcgac cgccggggtg tcgccgagct gagccgccag cccgtcggca
     1201 atgccggagg ccagcctgag ggtgcggctg tccgggcgct ggttgccaac gatggcgacg
     1261 acgttggggg tgaatccggg atggagggag ttctggttca tcggatcgtc ctgtcttgac
     1321 cgggggaagg agggtcgggg gccggacctt tcggcccggc ccccttgcct tgcgtccagc
     1381 cgggccgtct agcccaccgc ccagcggagt cgacttagac atttatgagt ttgtctagtc
     1441 aatagatcaa gatactacac gatcgactag aagacccgca atgggcagga gtctgtcgct
     1501 caattcccga ataggatcgg acaatggttg gcgaaccgcc aaggcccaag cagccgccag
     1561 gacgaattgg gaccctggcc cgccttttcc atccgaaggc cctgaatcgc aagcatcctc
     1621 atgtttccga ccgcttgagg acattcatgg cgactctgac ttttctggag ctcgttctga
     1681 tcttcgttct gttgatcgtc gcatcgacgg tccttctggg actcaccttc tggtggcttg
     1741 ctccaagcca gctctacaac agttattggg acgcgcttgg atcagccttt ttcaccctga
     1801 tcggcatgcc acccggctat ctggaccctg cagaattgag tggaagattc aaggtcgccg
     1861 taggggcggc tggactcttc tcgttgcttt tgccggccct tttcctcgga gccattgtct
     1921 tccgcctttt cgtcaggctg cgcatcttcg tcctcaagga aagaccaaca ctcacccgaa
     1981 agaaagtgag aggcaccgag ggctggtatc tggcgatccg tctttactcg tcgacagcga
     2041 tcctgatcgt cgatattgat ttcaaggtct acttgcggat gaagagctca aacggattac
     2101 tgctctcgaa cgaagagatc cgggtgacca acgccacctg gcccatcgcc gacactcatg
     2161 tcccctacac cctttacctt cccgtggacg acggcgatct tgatgccaac gagcacctca
     2221 tttcactgca gggtcacgat ctagagacct gcgaaagcgc gatcatccac atccgaggcg
     2281 cgtccccaca gcttgcgtcg gaagtgaatg aaacgcacac gctgaaggtt ccgttggatc
     2341 tctctccggc ggattttggc ggggtccagg ttctctacga cggccgtggc ccggattggg
     2401 tgggctggga cgaattcgaa gcagaccgac cgttgcaatg accgactggg tcttcggcta
     2461 cggatcgctc gtcaccgacc tggtcacaag ttcggacgag aatggctttg agtggttctg
     2521 ggcaaatctt ggcaaccacc ggcgggattg gggggccggc atggagaatg ctgctgccga
     2581 tgccgattgg aagtactacg tcgactctcg aggcacacgc cctgacatcg tggtcgcttt
     2641 tctcgacgct tacccccact ccggttccag catcaacgga gtcgtatttg ccgtagacga
     2701 gagtcgtctc cgggaaattg atcggcgcga agtgaggtac tcccgcgtcg acgtgactga
     2761 tctggtcacg acggccggtt ggggagacgg tcgggtctgg acctaccggg ggagcgagaa
     2821 atctcgcgaa gtcgcactcc gaggaaggcg agaagaccgg gccttcgtaa gcagggatta
     2881 tgcagcgcat tgccggctcg gattctccgt gggaggtgag caggcacttc accagtttca
     2941 attgagcacc gatcccctcg atctaccact ccgcgaactc acgatgtttc tttcggatcc
     3001 tggttgactc ttgcgcgtca gcccggttcg gtttcaacgt gagggccgac gaactggaat
     3061 agtccggcat cttgaagtca agtgtcccgt tccgggccgc ggtcgcggcc gtagttctgc
     3121 tcgccgcggt tgcgctggcc atcgacggct gcggcaagga cgaagagacc gagacgaccc
     3181 gcaccctgcg gatctatgta agccagccgc tgaagggtga ccgcggcggc gaggacatgc
     3241 tgcgggcggt cgagattgcg aatgaggacg agggcgggac gatcggcccg gtccggatcg
     3301 agatcgtcgg catgaacgat tccgacgaga gcggcaactg ggtcgcttcg ctggtccgcc
     3361 gcaacgcgcg gcgtgctgcc gccgacgact ccgccgtcgc ctacatcggt gatttcgatt
     3421 ccggcgcaac cgaggtcggg atgccgatcc tcaaccgcgc cgggatgctc caggtcagct
     3481 ccagcgccac cgcggtcaag ctgaccaagc ccctgcccga gctcggcgaa cagattcggc
     3541 cgaccggaat cagaaccttc ggccgggtgg ttcccaatga caacgtccag gccgcggccc
     3601 tggccctgtt catggaccag gagagcgtca acgaggtttt cgtggtcgat gacggcgaca
     3661 cctacggcga gggactgcgc ttccgcttcg agaaggtcgc ccggcacacc gggatcgaga
     3721 tcaccggcgg cgcgaccgtt acccgttcga tccagattgg cgacgtcgcg aaacgagtca
     3781 tcgggtccgg ggccgacgcc ctgctcttcg ccgggtcgaa ccttgatctg gcccgtgacc
     3841 tcttcgagac ggtgcaccgc gaggacaact tcatcaagct cttcggtggt gacggtctct
     3901 cgctttcctc cttcctcgga tcgctcggcg acaccgggct cgatacctac atcacggcgc
     3961 ctcagctccc ggccggcaat tacgcgcgga gcggtgaatc cttcctgcgt ggtttccagg
     4021 aacggtatgg acgaccg
//
LOCUS       DAOQMF010000063         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_11271_length_3997_cov_3.146880, whole genome shotgun sequence.
ACCESSION   DAOQMF010000063 DAOQMF010000000
VERSION     DAOQMF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11271_length_3997_cov_3.146880"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLV77_01740"
     CDS             complement(<1..234)
                     /locus_tag="PLV77_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC14600.1"
                     /translation="MAYVSFKAGLLAVLLAFAGLFTLTSTTRAEVVDVHLTARPATVK
                     IDDGVKLRAWTFNGTVPGPVIRATEGDTVRVTLT"
     gene            complement(401..1183)
                     /locus_tag="PLV77_01745"
     CDS             complement(401..1183)
                     /locus_tag="PLV77_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNC14601.1"
                     /translation="MNFTLEIWRQFDGADESEAGRFETFKVEGINPDASFLEMLDQLN
                     ERLSHEKKRTIAFDSDCREGICGACSLVIDGTPHGPQQVTSCQTYMRDFIDGQTIKVE
                     PFRANTFPVIRDLAVDRGALDRVIQAGGYISTTTGPQPEPNSMPVSPEVQERAMDAAI
                     CIGCGACVAACPNGAAMLFTGAKVTHLNILPQGQPERETRVIGMVEQMDQEGFGGCTN
                     YGECSRVCPQEISIDVIGMLNREYRKAKLSRPVKTDRAMTTQ"
     gene            complement(1180..3087)
                     /locus_tag="PLV77_01750"
     CDS             complement(1180..3087)
                     /locus_tag="PLV77_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNC14602.1"
                     /translation="MTLFDSNIPEGPLADKWQNWLDAHTLVGPRNRAGKKVIVIGTGL
                     AGSSCAASLGAQGFKVKSFCFQDTPRRAHSIAAQGGINAAKDYANEGDSAHRLFYDTI
                     KGGDFRSREANVMRLAQLSENIIDQAVAQGVPFNREYSGALATRSFGGVLVQRTFYTR
                     GQTGQQLLLGAYSALEHQVAAGNVEVFNRHEMQDVVVIDGEARGVIVRNLVTGEIERH
                     AADAVVLASGGYTNVYYLSTNAMGSNVTATWRAHKRGAYFANPCYTQIHPTCIPQSGE
                     YQSKLTLMSESLRNDGRIWVPRKAGDDRPPAGIPEAERYYYLEERYPAFGNLAPRDIA
                     SRAAKVVCDDGLGVGGTGRGVFLDFRDAIADQGKEAIERKYGNLFEMYTRITGDDPYE
                     TPMMIYPAPHYAMGGLWVDYELQTTIPGLFAIGEANFSDHGANRLGASALMQCLADGY
                     FISPHTVTNHLAKNHEPVSTSDSAFDEVENDVRGHVQELMDVGGSTSAQAFHREMGHV
                     MLDKCGMSRTAEGLDEALAEVRAIKEEFWRDLRVDPGDEEFNQSLEYANRVSDFFELG
                     ELMIQDARRREESCGGHFREEHQTDDGEALRDDEHFAKVTAWEYGGDNPVPHDEALNF
                     EDIKLATRSYK"
     gene            complement(3084..3812)
                     /locus_tag="PLV77_01755"
     CDS             complement(3084..3812)
                     /locus_tag="PLV77_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNC14603.1"
                     /translation="MIEFWRSSIGKKNIVAVTGAILIGYLILHMLGNLNSAFGPGANE
                     ARVDWYAGWLRDFGEPLLPHAFILWAVRVILLSALVIHITGIVQLTKRNKEARPVRYP
                     AKRLGRSVEATAMLGTGALILAFIIFHILQFTTLSIHVTPVAEGDVYANLYYAFQKWY
                     FVAIYLGALLFIGMHLRHGVWSFFQTLGLDSPSRNAKLRHGSTALVVILVVGFGSIPV
                     LFFTDVLDPPRTEAVLTTLAGVPL"
BASE COUNT          663 a         1225 c         1397 g          712 t
ORIGIN      
        1 ggtgagagtc acccggacgg tgtctccctc ggtggcgcgg atgaccgggc ccggcaccgt
       61 cccgttgaaa gtccaggccc gcaacttcac tccgtcgtcg atcttcaccg tggccgggcg
      121 ggccgtcagg tgaacgtcga ccacctcggc gcgggtggtg gaggtaaggg tgaacaggcc
      181 ggcgaaggcc aacaggaccg caaggagacc agctttaaac gagacgtaag ccatggagcg
      241 accgtatacc ggtcacctga cttggacaac cgtaagaact acggaaccgg gttgcgggaa
      301 actacgcact cccgcgcccg gcggggcgcg ccgtcagcga cagacagcgt tgaggttatt
      361 cgctccatgt gcggataact tcaatgcagt tggtgaggcg ttactgggtg gtcattgccc
      421 ggtcggtctt gaccgggcgg ctcagcttgg ccttgcggta ctcgcggttc agcatcccga
      481 ttacgtcgat cgagatctcc tgggggcaga cccgggagca ctcgccgtaa ttggtgcagc
      541 cgccgaagcc ctcctggtcc atctgctcga ccatgccgat cacgcgggtt tcgcgctccg
      601 gctggccctg gggcaggatg ttcaggtggg tgaccttggc cccggtgaag agcatcgccg
      661 cgccgttcgg gcaggccgct acgcaggcgc cgcaaccgat gcagatcgcc gcgtccatcg
      721 cccgctcctg gacttccggt gaaaccggca tcgagttcgg ttccggctgg ggaccggtgg
      781 tggtcgagat gtagccgccg gcctggatca cccggtcgag cgcgccgcgg tcaaccgcga
      841 ggtcgcggat caccgggaag gtgttggccc ggaacggctc gaccttgatc gtctggccgt
      901 cgatgaagtc gcgcatgtag gtctggcagg aagtgacctg ctgagggccg tgcggagtgc
      961 cgtcgatgac cagcgagcag gcgccgcaga tgccctcgcg gcagtcggag tcgaaggcga
     1021 tggtgcgctt cttctcgtga ctgaggcgct cgttgagctg atcgagcatc tcgaggaagg
     1081 acgcatcggg gttgatgccc tcgaccttga aggtctcgaa gcggcccgcc tcgctttcat
     1141 cggcaccgtc gaactgacgc cagatctcaa gggtgaagtt cacttgtagc tcctcgtcgc
     1201 cagcttgatg tcctcgaagt tcagagcctc gtcatgggga accggattgt cgccaccgta
     1261 ctcccaggcg gtgaccttgg cgaaatgctc gtcgtcgcgg agcgcctcgc cgtcatcggt
     1321 ctggtgctcc tcgcggaagt ggccgccgca ggattcctcg cgccggcggg catcctggat
     1381 catcagctcg ccgagttcga agaagtccga gacccggttc gcgtactcga gcgactggtt
     1441 gaactcctcg tcacccgggt cgacccgcag gtcccgccag aactcttcct tgatcgcccg
     1501 cacttcggcc agggcctcgt cgaggccttc ggcggtacgg gacatgccgc acttgtcgag
     1561 catcacgtgg cccatctcgc ggtggaaggc ctgggcggag gtgctgccac cgacgtccat
     1621 cagctcctga acgtgacccc ggacgtcgtt ctcgacctcg tcgaaggcgg aatcggaagt
     1681 gctgaccggc tcgtggttct tggcgaggtg gttggtcacc gtgtgggggg agatgaagta
     1741 gccgtcggcc aggcactgca tcagggccga ggcgccgagg cggttggcgc cgtggtcgga
     1801 gaagttcgcc tcgccgatcg cgaacaaacc ggggatcgtg gtctggagtt cgtaatcgac
     1861 ccagagcccg cccatcgcgt aatgcggggc cgggtagatc atcatcggcg tctcgtacgg
     1921 gtcatcgccg gtgatccgcg tgtacatctc gaagaggttg ccgtacttgc gctcgatcgc
     1981 ttccttgccc tgatcggcga tcgcgtcgcg gaaatcgagg aagacgccgc ggccggtgcc
     2041 gccgaccccg aggccgtcat cacagaccac cttggcggcg cgggaggcga tgtcacgcgg
     2101 ggcgaggttg ccgaaggccg ggtagcgctc ttccaggtag tagtaccgct cggcctcggg
     2161 gatcccggcc ggcggacggt cgtcaccggc cttgcgggga acccagatcc ggccgtcgtt
     2221 gcggagcgac tcgctcatca gggtcagctt cgactggtac tcgccggact gcgggatgca
     2281 ggtcgggtgg atctgcgtgt aacaggggtt ggcgaagtat gcgccgcgct tgtgggcccg
     2341 ccaggtggcg gtcacgttcg agcccatcgc gttggtcgag aggtagtaga cgttggtgta
     2401 gccgcccgag gcgagcacta ccgcgtcggc cgcgtggcgc tcgatctcac cggtgaccag
     2461 gttgcgcacg atcacgccac gggcttcgcc gtcgatgacc accacgtcct gcatctcgtg
     2521 ccggttgaag acctcgacgt tgccggccgc gacctggtgc tcgagggccg agtaggcgcc
     2581 gagcagaagc tgctggccgg tctggccgcg ggtgtagaag gtgcgctgga ccagcacccc
     2641 gccgaaggaa cgggtggcga gcgcgccgct gtattcgcgg ttgaagggca caccctgggc
     2701 gaccgcctgg tcgatgatgt tctccgagag ctgggcgagc cgcatcacgt tcgcttcgcg
     2761 ggagcggaag tcgccgccct tgatcgtgtc gtagaagagc cggtgggcgc tgtcgccctc
     2821 gttggcgtaa tccttggctg cgttgatccc gccctgggcg gcgatcgagt gggcgcggcg
     2881 cggcgtgtcc tggaagcaga agctcttgac cttgaaaccc tgggcgccga gactggccgc
     2941 gcaggaggag ccggccaggc cggtaccgat cacgattacc ttcttgccgg cccggttgcg
     3001 gggaccgacc agggtgtggg cgtcgagcca gttctgccac ttgtcggcca gcgggccttc
     3061 cgggatgttc gaatcaaaga gggtcacagg ggaactcccg caagcgtggt gagcaccgct
     3121 tcggtcctcg gcggatcgag cacgtcggtg aagaagagga ccgggatcga accgaagccg
     3181 accacgagga tcacgaccag ggcggtcgag ccgtgccgga gcttcgcgtt gcggctcggc
     3241 gaatcaaggc cgagggtctg gaagaagctc cagacgccgt gccgcagatg catgccgata
     3301 aagagcagcg cccccaggta gatcgccacg aaataccact tctggaaggc gtagtagagg
     3361 ttcgcgtaga cgtcaccttc cgcgaccggg gtcacgtgaa tcgaaagcgt cgtgaactgg
     3421 aggatgtgga agatgatgaa ggcgaggatc agggcgccgg tcccgagcat cgcggtcgcc
     3481 tcgacgcttc ggccgagccg tttggccgga taccggaccg gccgcgcttc cttgttgcgt
     3541 ttggtcagct gaacgatgcc ggtgatgtgg atgaccaggg cgctcagcag gatgacccgg
     3601 accgcccaga ggatgaaggc atggggcagc agcggttcgc cgaagtccct gagccagccg
     3661 gcgtaccagt caacccgggc ctcgttggcg cccggcccga aggccgagtt gaggttgccc
     3721 agcatgtgga ggatcaggta accgatcaga atcgcgccgg tcacggcgac gatgttcttc
     3781 ttgccgatgc tggatcgcca gaactcgatc atctggtgac tgatggccgg acggacttga
     3841 tcacgggaag ccgattctat tgcccggcgc taacgggcgg cttcccgcaa ctggctttgg
     3901 ggagcctaat tccagcccgc tggcggggaa gctactcgcg ggaggtgatg cggtccagct
     3961 cgacctcgcc accttcgtcg gtgtcctcca tcaccac
//
LOCUS       DAOQMF010000064         3977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_11371_length_3977_cov_3.611678, whole genome shotgun sequence.
ACCESSION   DAOQMF010000064 DAOQMF010000000
VERSION     DAOQMF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11371_length_3977_cov_3.611678"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLV77_01760"
     CDS             complement(<1..741)
                     /locus_tag="PLV77_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14604.1"
                     /translation="MLEPIALAPQHDGPQTVGEVSPAAHRLGVRPGMGPGEAVDICPE
                     LALITPDPTRASQIWEAVLGRIESTGAEVESVRDGEAFFDATGVELLHGGLEGVLDAV
                     SEKLGPDFLLAAAPTRLAALALAGRQPAGKSSGHGRVLPPEELAAFLDPLPLATLLDR
                     LPGPAGEVRRMLESMRRLGLGTLGDLVALPADAVADRFGPAGLAARDLALGLEGPIRP
                     RTPFEQITEWLELPDAASGIHLEGGLTIL"
     gene            977..2176
                     /locus_tag="PLV77_01765"
     CDS             977..2176
                     /locus_tag="PLV77_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC14605.1"
                     /translation="MTQPRLSRRQVMKGAAGAAMSVSLAQMIAACGLPGSRDTGWTAG
                     TDWNAWWRDQQKNGSFNFASWPLYIDTDGGKHPSLDKFTKETGIDVTYSPVIQDNASF
                     FSQISPVLQNGQSIGYDLIVISNGWQLTQLMQNKWLVPLDHSRMPNFRKYAGTVATDL
                     AYDPHLTYCAVWQAGVTAIAYDEKQVDGPIDSLDALFDPKYKGKVGMLSDPDELGTAG
                     LLALGINPQTSTYADWQKSADLLTRQRDDGIVRQYYDNSYIKALEDGDLAISQAYSGD
                     VFQAQNSGYPNLKFVIPKQGGVIWRDNMVIPYHAENPVDAIEWINFAYNPEIAALIAD
                     WVNYICPVPAAKPIIANQLDDPTVANSPLVFPSEAELSRLKEYPVTETLKTHEQWVGL
                     FDPIIQS"
     gene            2205..3053
                     /locus_tag="PLV77_01770"
     CDS             2205..3053
                     /locus_tag="PLV77_01770"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14606.1"
                     /translation="MKSRIGPYLQSAPAMLWLALFFIVPLLAIVSISLMTGNSIDGYT
                     LTWNFSVFPDALDQYSAQLGRSFLYGGISTLLALIAMYPVAYWIAFHGGRRKSIFLFL
                     LLLPFLVSFIIRILTWQFILSDEGIVLGTLKDLGILGENAHILSTPVAVITGLTYDAL
                     PWMALPIYVALEKIDRDLVEAAGDLYASGYQQFRRIILPLSAPGIYAGILLVGIANIG
                     DYLSAQILGGPSTTMIGNIIQTQYVQNGDYPVASALSLMLMLALLLCVFVYARVFGTR
                     TIQEYA"
     gene            3053..3904
                     /locus_tag="PLV77_01775"
     CDS             3053..3904
                     /locus_tag="PLV77_01775"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14607.1"
                     /translation="MAGKGISSADSARPVRRFPWTRFVLPSYVFVVMAYTLVPIAVMI
                     LYTFNKAPNQRLTFSWHGFTTEWYSKLFEVQDLTQALWHSLEVAVISSIIATALGTPA
                     AIALARRRFRGRGAIDLVVVTNLTAPTVVVGASLLGFFIALSVPRGLVTILIAHVAFN
                     IAIVIIVVQARVSGFDESLEEAAADLGAPPWITFWKVTMPLILPGIFSAFLLSFALSI
                     DDYIITSFVAGQTLTFPLWVAGAVKVGIPPQVFVMGTLIFVSGVLIALASLILQRWGD
                     HGETVRT"
BASE COUNT          693 a         1359 c         1219 g          706 t
ORIGIN      
        1 aagaatggtc agcccgcctt cgaggtggat gccgctggcc gcgtcgggca gctccagcca
       61 ttcggtgatc tgctcgaagg gggtgcgagg gcgaatcggc ccttccaggc cgagggccag
      121 gtcacgggcg gccagcccgg cggggccgaa acggtcggcg accgcgtcag ccggcagggc
      181 gacgagatcc cccagggttc ccagccccag ccggcgcatg gattcgagca tccggcgaac
      241 ctcgccggcc ggcccgggca ggcggtcgag cagggtcgcg agagggagcg ggtcgagaaa
      301 ggcggcaagc tcctccggcg gcaggacccg gccatgaccg gaactcttgc cggccggttg
      361 cctgccggcc agggcgaggg cggccagacg ggtcggagcg gcggcgagga ggaagtccgg
      421 cccgagcttt tcgctgaccg catcgagtac gccttcgagc ccgccgtgga gcagctcgac
      481 accggtggcg tcgaagaagg cttcgccatc ccggaccgat tcgacctccg caccggtcga
      541 ttcgattcgc cccaggacgg cttcccagat ctggctggcc cgggtcggat cgggagtgat
      601 caaggcaagt tccggacaga tgtcaactgc ctcgccggga cccatgccgg gccttacccc
      661 cagccggtgt gcggccgggg aaacctcgcc gaccgtctgc gggccatcat gctgcggggc
      721 cagggcgatc ggctccagca agcggttttg acccttttcg agggctgccc ggagagccag
      781 aaggggaagc aggacacaaa cgaacatata cgaacatatg ttcgcatatc cggccgggcc
      841 aggtcaagca gatcgtcata cgaaagggga ctttccggac ttttcggccg accggggcac
      901 gaaagcggat cgctatcgtg aatccgtggg caaggagcag agatacatcc atcgtgatcc
      961 ggcctggatc agggggatga ctcagcccag gctttcccgc cggcaggtca tgaagggcgc
     1021 ggccggcgcc gcgatgagcg tcagcctcgc ccagatgatt gccgcctgcg ggctgcccgg
     1081 cagccgcgac accggctgga cggccggtac cgactggaac gcctggtggc gggaccagca
     1141 gaagaacggc tccttcaact tcgccagctg gccgctttac atcgacaccg acggcggcaa
     1201 gcatccttcg ctcgacaaat tcacgaagga aacgggaatc gacgtcacct acagcccggt
     1261 aatccaggac aacgcttcct tcttctccca gatcagcccg gtcctgcaga acggccagtc
     1321 gatcggctac gacctgatcg tgatctcgaa cggctggcag ctgacccagc tgatgcagaa
     1381 caagtggctg gtcccgctcg accattcgcg gatgccgaac ttcaggaagt acgccgggac
     1441 ggtcgccacc gacctggcct acgacccgca cctgacctac tgcgcggtct ggcaggccgg
     1501 ggtaacggcg atcgcctacg acgagaagca ggtcgacggt ccgatcgatt ccctcgacgc
     1561 cctcttcgac ccgaagtaca agggcaaggt cgggatgctc agcgacccgg acgagctcgg
     1621 tactgccggc ctgctcgctc tcggcataaa tccgcagacc tcgacctacg cggactggca
     1681 gaagtccgcc gacctgctga ccagacagcg cgacgacggg atcgtccgcc agtactacga
     1741 caacagctac atcaaggccc tcgaagatgg cgacctggcg atcagccagg cctattcggg
     1801 ggacgtcttc caggcccaga actccggcta tcccaacctc aagttcgtga tcccgaagca
     1861 gggcggggtg atctggcggg acaacatggt gatcccgtac cacgccgaaa acccggtcga
     1921 cgcgatcgag tggatcaact tcgcctacaa cccggagatc gcagccctga tcgcggactg
     1981 ggtcaattac atctgcccgg tgccggccgc caagccgatc atcgccaatc agctcgacga
     2041 tccgaccgtg gccaacagcc cgctcgtctt tccatccgaa gccgagcttt cccgactcaa
     2101 ggaatacccg gtgaccgaga ctctcaagac ccatgagcaa tgggttggac tcttcgaccc
     2161 gatcatccag tcatgagcgc cccggccagc aagcggaggt cacagtgaag tcccggatcg
     2221 ggccgtacct gcagtcggca ccggccatgc tctggctggc gctcttcttc atcgtgccgc
     2281 tgctggcgat cgtctcgatt tccctgatga ccggcaattc gatcgacggc tacaccctga
     2341 cctggaactt ctcggtcttt cccgacgcgc tcgaccagta ctcggcccag ctcggccgct
     2401 ccttcctcta cggcgggatc tcgaccctgc tggccctgat cgcgatgtat ccggtcgcct
     2461 actggatcgc ctttcacggg ggcaggcgca agtcgatctt cctcttcctt ctgctcctgc
     2521 ccttcctggt cagtttcatc atccgcatcc tgacctggca gttcatcctc tccgacgagg
     2581 ggatcgtgct cggcaccctg aaggacctcg ggatccttgg cgagaacgcc cacatccttt
     2641 cgactccggt cgcggtgatc accggactca cctatgacgc gctgccctgg atggcgctgc
     2701 cgatctacgt tgccctggag aagatcgacc gtgacctggt cgaagcggcc ggtgacctct
     2761 acgcctccgg ctaccagcag ttccgacgga tcatccttcc gctctcggcc ccgggcatct
     2821 acgccggcat cctgctggtc gggatcgcca acatcggcga ttacctgagc gcccagatcc
     2881 tcggcggtcc gagcaccacg atgatcggca acatcatcca gacccagtac gtgcagaacg
     2941 gtgactaccc ggtcgcctcg gccttgtcgc tgatgctgat gctggcgctc ttgctttgcg
     3001 tcttcgtcta cgcccgggtc ttcggcaccc ggaccatcca ggagtacgcc tgatggccgg
     3061 caagggcatc tcctccgccg acagcgcccg cccggtccgc cgcttcccct ggacccgctt
     3121 cgtcctgcct tcctatgtct tcgtggtcat ggcctacacg ctggtcccga tcgcggtgat
     3181 gatcctctac accttcaaca aggcgccgaa ccagcggctc accttctcct ggcacggctt
     3241 cacgaccgag tggtactcga agctcttcga ggttcaggac ctgacccagg ctctctggca
     3301 ctcactcgaa gtggcggtga tcagttcgat catcgccacc gcgctcggca ccccggccgc
     3361 gatcgccctg gcccgccgca ggttccgggg tcgcggggcg atcgacctgg tggtggtcac
     3421 caacctgacc gccccgaccg tggtggtcgg agcgtcgctg ctcggcttct tcatcgccct
     3481 ttcggtgccg cgcgggctgg tcacgatcct gatcgcccat gtcgccttca acatcgcgat
     3541 cgtgatcatc gtcgtccagg ccagggtcag cggtttcgac gaatccctgg aggaggccgc
     3601 ggccgacctc ggggcaccac cctggatcac cttctggaag gtgaccatgc cgctgatcct
     3661 gcccggcatc ttctctgcct tcctgctctc cttcgcgctc tcgatcgacg actacatcat
     3721 caccagcttc gtcgccggtc agaccctgac tttccccctg tgggtggccg gcgcggtcaa
     3781 ggtcggcatc ccgccacagg tattcgtgat gggcaccctg atcttcgtca gcggggtcct
     3841 gattgccttg gccagcctga ttctgcaacg ctggggtgac catggagaga cggtgaggac
     3901 atgagcagac agggtgcgca atgcgactgg tgcggggtgg agctgaccga ggagatgggc
     3961 taccggctgc tgtggcc
//
LOCUS       DAOQMF010000065         3961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_11465_length_3961_cov_3.923195, whole genome shotgun sequence.
ACCESSION   DAOQMF010000065 DAOQMF010000000
VERSION     DAOQMF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11465_length_3961_cov_3.923195"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLV77_01780"
     CDS             complement(<1..391)
                     /locus_tag="PLV77_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNC14608.1"
                     /translation="MNDLATTLCFPALGGEVLIQAEGPGSDLAVEEARELVLDLHRRL
                     TRFEPSSELCQLNRSSDRAVKASPLLLRFADEVHRAGSNSGGLVDATLLDQVEAAGYR
                     DSINPDDPAGPGPSSPELEPPARRWSWS"
     gene            complement(388..744)
                     /locus_tag="PLV77_01785"
     CDS             complement(388..744)
                     /locus_tag="PLV77_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14609.1"
                     /translation="MNELTPKEMAEAKKLARRVRVGNIRRRIAYGATLMVAVFSGAIL
                     FRSLEQQTGTAATNVSAVTRSDESDDSEGLSALVTQVSSAFGDDGEEGSPSTTTGTVT
                     GSTTTSSAPPLVTSQS"
     gene            complement(759..1166)
                     /locus_tag="PLV77_01790"
     CDS             complement(759..1166)
                     /locus_tag="PLV77_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14610.1"
                     /translation="MSETHDDQKPDQNDPATTELTPEKGWSATTRRLADNPVGRWVAV
                     GAAVVVALFSGMLIGRATGQDPAEAGPASFGRPDGDGPPAGGQVPFHGDDDFGGQSFG
                     DDDSYEQPEGGDDEHSESSSGGSSPSPLETGQS"
     gene            1269..1943
                     /locus_tag="PLV77_01795"
     CDS             1269..1943
                     /locus_tag="PLV77_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC14611.1"
                     /translation="MRILLVEDEKAIADFIARGLEAEGHAVTWVADGIDGEALARTGR
                     FDLILLDLMLPGKDGLEVLASLRREDGSTPVILLTAKGEVADRVAGLDAGANDYVTKP
                     FAFEELVARIRAQLRSPAQGSATELRTGDIELNLLRRRVKRGDVEVLLSAREFDLLAY
                     MMRHPGQVLSREQLLNGVWGYDYDPGTNIVGVYIAYLRRKLAMEGRPDPIETLRSVGY
                     RLRDGR"
     gene            1946..3418
                     /locus_tag="PLV77_01800"
     CDS             1946..3418
                     /locus_tag="PLV77_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC14612.1"
                     /translation="MPHPGLRGKLAISIALVLLIALGVTYFAVYRGTGSELKDRTESE
                     LDKGVSQIAARLKVGGADTPAEHTARAHQITSAEGFGPNARLVSISIEGGGTASNQPE
                     LVGPGLEDHHSDGEQKHAGHESGDDSEDEPAEKKQDADRILGSAPGYSTIEVEDAGKV
                     RILTREVELPGGVKATIRVGQPLAPAENALEGLSDTFVIVGIATLLLGALAGWLLAST
                     ATRPMRRMAQVAEGVDAGDLSARMPVEGTRDEARRLAESFNGMLDRLEDAFDRQRAFV
                     ADASHDLRTPLTVVRGQAEVLARNPNPSREEVTRVYELVSQATARMELMVDDLLLLAR
                     AESSEGLRLEPLELEPLISAEVEGMAFENNRDLVAGVVTGRRVNIDREQVARLLSNLI
                     SNAIAHTEEGGRIEVSATDHGSKVVLAVDDDGPGVPESDRERVFDRFARLDESRSSSL
                     GGSGLGLAIVRAIAEAHGGEARCVDSPLGGARFEIGFPAA"
     gene            3456..>3961
                     /locus_tag="PLV77_01805"
     CDS             3456..>3961
                     /locus_tag="PLV77_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNC14613.1"
                     /translation="MRFSADQPLNVQPATVRDYRELARRRLPRQLFDYIDGGAYEEVT
                     ARANETDLAKVLLRQRVMRDVSVREQATEVLGQKLSMPVILGPVGLAGMFATRAEVQA
                     ARAAEAAGVPFIESTVSICSIEEVAEATSVPPWFQLYVMRDRGYAEKLMARAEAVGAP
                     VLALTIDLA"
BASE COUNT          642 a         1260 c         1385 g          674 t
ORIGIN      
        1 ccgaccagct ccaacgccgg gcggggggct cgagctcggg actcgaaggt ccgggtccgg
       61 ccgggtcgtc gggattgatc gagtccctgt atcccgcggc ttcaacctgg tcgaggagtg
      121 tggcgtcaac caggccgccg ctgtttgatc ccgcccggtg gacttcgtct gcgaatcgga
      181 ggaggagtgg gcttgccttc acggcccggt cgctgctgcg gttgagctgg cagagctcgc
      241 tggatggttc gaagcgggtc agccggcggt gcagatccag gaccagctcc cgggcctctt
      301 cgaccgcgag gtcggaaccc gggccttccg cctggatcag gacttcgccg ccgagggcgg
      361 ggaaacagag ggttgtggcc aggtcattca tgactggctg gtgaccagcg ggggagcgct
      421 tgaagtggtc gtggagccgg tcaccgtgcc cgtcgtggtc gaaggtgaac cttcttcacc
      481 gtcatcgccg aatgccgatg aaacctgcgt gacgagcgcg ctcaggccct cggagtcatc
      541 gctctcgtcg ctgcgggtca cggccgaaac gttggtcgcg gcggttcccg tctgctgttc
      601 cagcgatcgg aagaggatgg cgccgctgaa caccgcgacc atcagcgtcg ctccgtaggc
      661 gatccggcgg cggatgttcc cgacccgaac ccggcgggcc agcttcttcg cctcggccat
      721 ttcttttggg gtcagttcat tcatgctcat gtacctcctc aggactgtcc ggtctccagc
      781 ggcgagggcg aggagccgcc ggaggagctt tcactgtgtt cgtcatcgcc cccctctggc
      841 tgttcgtagc tgtcgtcgtc gccaaagctc tggccgccga agtcgtcgtc gccgtggaag
      901 ggcacctggc caccagccgg aggtccgtcg ccgtccggcc ggccgaaaga tgcgggtccg
      961 gcctcggccg ggtcctgccc ggtggcccgc ccgatcaaca tcccgctgaa gagcgcaacg
     1021 acgacggcgg cacccaccgc gacccagcgg cccaccgggt tgtcggcgag cctgcgggtt
     1081 gtcgccgacc accccttttc cggggtcagt tccgtggttg cgggatcgtt ctgatcgggc
     1141 ttctggtcgt catgcgtttc gctcatgtcc gaaaggttga tccagccggc ttggaggatc
     1201 cgtaacgaaa catgagagtt ctcttagaaa cgcgatcccg gccggattcg cagttacgat
     1261 cggcaccgat gcgcatcctc ctggtcgaag acgaaaaggc gatagccgac ttcatcgccc
     1321 gtggccttga agcggagggt catgcggtga cctgggtcgc ggacgggatc gacggggaag
     1381 ccctcgccag aaccggacgc ttcgacctga tcctgctcga cctgatgctg cccggcaagg
     1441 acggcctcga ggtgctcgct tcgctgcgcc gggaggacgg gtccacgccg gtgatcctgc
     1501 tcaccgcgaa gggggaggtc gccgaccggg tggccggact cgacgccggc gcgaacgact
     1561 acgtgacgaa gccattcgcc ttcgaggaac tggtcgcgag gatcagggcc cagctgagaa
     1621 gcccggccca gggcagcgcg accgagctca ggaccggtga catcgaactg aatctgctgc
     1681 gccggcgggt caagcgcggc gatgtcgagg tgttgctctc ggcccgtgaa ttcgacctgc
     1741 tcgcctacat gatgcggcat cccgggcagg tgctttcccg ggagcagttg ctgaacggag
     1801 tctggggcta tgactacgac ccggggacca acatcgtcgg ggtttacatc gcatacctgc
     1861 ggcgcaagct ggcgatggaa ggccggccgg acccgatcga aacccttcga tcggtcggct
     1921 accggctgcg tgacggccgg tgaacatgcc ccatcccgga ctgagaggca agctcgcgat
     1981 ctcgatcgcc ctggtcctgc tgattgccct gggggtcacc tacttcgcgg tctatcgagg
     2041 caccgggtcc gagctgaagg accgcaccga gtcggagctg gacaagggag tcagccagat
     2101 cgccgcgagg ctgaaggtcg gtggcgcgga cacaccggca gaacacactg ccagggccca
     2161 tcagatcacc tcggcggagg gcttcggccc gaacgcccgg ctggtttcga tctcgatcga
     2221 aggcggtggc actgccagca accagccgga gctggtcggg ccgggtctcg aagatcacca
     2281 ttccgacggc gagcagaaac acgccggcca cgaaagcggt gacgacagcg aagacgagcc
     2341 cgccgagaaa aagcaggatg cggaccggat cctggggtcg gctccgggct actcgacgat
     2401 cgaggttgaa gacgccggca aggtccggat cctgacccgg gaggtcgaac tccccggggg
     2461 cgtcaaggcg acgatccggg tcgggcaacc gctggctccg gccgagaacg cgctcgaagg
     2521 cctcagcgat accttcgtca tcgtcggcat cgcgaccctg ctgctcggcg cgctggccgg
     2581 ctggcttctg gccagtaccg ccacccgccc gatgcggcgg atggcccagg tcgccgaagg
     2641 agtcgacgcg ggagatctct ccgcgaggat gccggtcgaa gggacccggg acgaagcccg
     2701 ccggctggcc gaatcattca acgggatgct cgaccgtctc gaggatgcct tcgaccgtca
     2761 acgggctttc gtcgccgacg cttcgcatga cctgcggacc cccctgaccg tggtgcgggg
     2821 ccaggcggag gtgcttgccc ggaacccgaa cccctcccgg gaagaggtca cccgggttta
     2881 cgagctcgtt tcccaggcga ccgcacggat ggagttgatg gtcgatgacc tgctgctgct
     2941 ggccagggcc gaatcctccg aaggcctccg gctcgagccg ctggaactgg agccgctgat
     3001 ttcggccgag gtggaaggca tggcctttga aaacaatcgc gacctggtgg ccggagtcgt
     3061 caccgggcgc cgggtgaaca tcgaccgcga acaggtcgcc cggctgctct cgaacctgat
     3121 ctcgaacgca atcgcccaca cggaagaagg gggcaggatc gaggtgtcgg ccaccgacca
     3181 tggatcgaag gtggttctgg ccgtggacga cgacggaccc ggcgtgcccg aaagtgaccg
     3241 ggagcgggtc ttcgaccgtt ttgcgcggct ggatgagtcg cgcagcagtt cgctgggcgg
     3301 ttccgggctc ggtctggcca tcgtccgggc gatcgccgag gcccatggag gtgaagcgag
     3361 gtgtgttgac tcgccactgg ggggtgcccg cttcgagatc gggtttccag ccgcctgagc
     3421 cgggggcttc ggccttcagc cggtagtttg ccgccatgcg cttctccgct gatcagcccc
     3481 tgaacgtcca gccggccaca gtgcgtgatt accgggaact cgcccgccgg cgtttgccgc
     3541 ggcagctctt cgattacatc gacggtggcg cctatgaaga ggtgactgca cgggcgaacg
     3601 aaaccgacct cgcaaaggtt cttctccgtc agcgggtgat gcgggacgta tcggtccggg
     3661 agcaggcgac cgaggtcctc ggccagaagc tttcgatgcc ggtgattctc gggccggtcg
     3721 gcctggccgg gatgttcgcg accagggccg aggtgcaggc ggcccgcgcg gcggaagcgg
     3781 ccggggttcc gttcattgag tcgaccgttt cgatctgctc gatcgaagag gtggccgaag
     3841 cgacctccgt gccgccctgg ttccagcttt atgtgatgcg ggaccgcggc tacgccgaga
     3901 agctgatggc gagggccgag gcggtcggcg ctccggttct ggccctgacc attgacctgg
     3961 c
//
LOCUS       DAOQMF010000066         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_11479_length_3958_cov_3.736100, whole genome shotgun sequence.
ACCESSION   DAOQMF010000066 DAOQMF010000000
VERSION     DAOQMF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11479_length_3958_cov_3.736100"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PLV77_01810"
     CDS             <1..1128
                     /locus_tag="PLV77_01810"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNC14614.1"
                     /translation="GVNALDGLLVADFSRVLAGPLVSMTLADFGADVIKVEPPGGDET
                     RAWSPPVDAQGRATFFLAVNRNKRSVELNLKDPEDLELAKELVGRADVLVENFRPGTM
                     DRLGLGYEEMAAANPGLVYCSISGFGSKEGAALPGYDPLVQAMSGMMSVTGPPEGDPS
                     KVGVALVDVIAGLNATIGVLIALQERAKSGQGQKVESNLLSSALAALENQASAWLNTG
                     VLPVRLGNVHPSIEPFTTFDASDGPLMICVGNDRQFKALCEVLGEPELSSDPRYVDNF
                     DRVEHRVELEAELNELFSRRTVEEWVTALHEARVPAGPVNTIDRGFAVAEALGLDPID
                     EIDGVRTPASPIRLGRTPAETRLPPPAENAHSEEIRDWLRS"
     gene            1137..1835
                     /locus_tag="PLV77_01815"
     CDS             1137..1835
                     /locus_tag="PLV77_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14615.1"
                     /translation="MEENEEKAPRPVAVWVGGTLAYAVLVLALGLILTGINPDSSVFP
                     PLLTALAGLFVATAINVRPASSGDADPDWNRFQVHVIVVAITSMVLSIGLVMCALAFP
                     GELIFQLAFWGAALAILIGAAFLLAVVWERLSTEGSPPSPASEPEPKPDPDPEPEPEP
                     EPEPAPEAEPAPESGKVKAAKKELRRAPQTDLVAATLKKSKKTKKPAKKSPKDPKASK
                     APKKPKKKDSKKKK"
     gene            1856..2845
                     /gene="rlmN"
                     /locus_tag="PLV77_01820"
     CDS             1856..2845
                     /gene="rlmN"
                     /locus_tag="PLV77_01820"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNC14616.1"
                     /translation="MDLTLLEETMAGLEQPAYRTGQVWEWLARGAGSFDEMTNLPASL
                     RQELEGRLPISTLEVDASMKSVDGTEKALFLTADGRPVEAVLMRYRDGRRSLCLSSQS
                     GCPLTCTFCATGTMKFGRNLTESEILDQALHFRRIEPVNHAVFMGMGEPMMNLDNVLA
                     VCERLPEVGIANSHTTVSTVGWQPGIERMTTEGPAVRLALSLHAPNDKLRSEIMPVND
                     RYPMEDVVAACREWRHTRKRKVFIEYLMLDGVNDSVELAHELADLLLPKNDFKVNLIP
                     YNPSGTGYQGSPREAIDRFRDTLMKRGIHATVRLTRGRDIDAACGQLAAKAAA"
     gene            complement(2955..3446)
                     /locus_tag="PLV77_01825"
     CDS             complement(2955..3446)
                     /locus_tag="PLV77_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNC14617.1"
                     /translation="MTEILNVLVEVPKGSRNKYEYDEELGAIKLDRFLFSSMVYPVDY
                     GMVPNTMGADGDPLDAMVCVSEPTFPGCIIPAIPLGLFRMTDDGELDDKILCVPADDP
                     NWNFIESLDQLSSQLKDEISHFFAVYKQPEGKHVEVDGWYPLESALEVLELSRERFAS
                     GTA"
     gene            complement(3518..>3958)
                     /locus_tag="PLV77_01830"
     CDS             complement(3518..>3958)
                     /locus_tag="PLV77_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNC14618.1"
                     /translation="VVFKRPPHTGVGMKSHKPPRNQMVVRIDPVPGARMAFVAKAAGI
                     EDFEEADLDVLFEKEPGAEPEPYERLLDDAIHGRYDLFTKFSMVEETWRIVQPLLDDP
                     PPVVPYEVGSWGPEEADRLVKGICDWDEPWRPTEDPRHHMLLGG"
BASE COUNT          719 a         1290 c         1296 g          653 t
ORIGIN      
        1 ggcgtgaatg ctctggacgg actcttggtg gcggacttca gccgggtgct ggccggtccg
       61 ctcgtttcga tgaccctggc cgatttcggt gccgacgtga tcaaggtcga gccgcccgga
      121 ggggatgaga caagggcctg gtcgccgccg gtcgacgccc agggcagggc gaccttcttt
      181 ctcgccgtca accgaaacaa gcggtcggtc gagctcaacc tcaaggaccc cgaggatctg
      241 gaactggcaa aggaactggt cgggcgggcc gacgtgctgg tcgagaactt ccggcccggc
      301 acgatggacc ggctcggtct cggttacgag gaaatggccg cggccaaccc cgggctcgtc
      361 tactgctcga tcagcggctt cggctcgaag gagggggccg ccctgcccgg ctacgacccg
      421 ctggtccagg cgatgagcgg gatgatgtcg gtgaccggtc ctcccgaagg tgacccgagc
      481 aaggtcgggg ttgctctggt tgacgtgatc gcgggcctca acgcgacgat cggggtgctc
      541 atcgcactgc aggaaagggc gaagtcgggg cagggccaga aagtggaaag caacctgctg
      601 agttcggccc tggcggcgct cgagaatcag gcgtcggcct ggctcaacac cggggtgctg
      661 ccggtccggc tgggcaacgt ccatccgagc atcgagccgt tcaccacttt cgacgcgagc
      721 gacggtccgc tgatgatctg cgtcggcaac gaccgccagt tcaaggcgct ttgcgaggtg
      781 ctcggcgagc ctgagctgtc atcggacccg cgctatgtcg acaacttcga ccgggtcgag
      841 caccgggtcg agctcgaggc cgaactgaac gaactgttct cccgccggac cgttgaagag
      901 tgggtgaccg ccctgcatga ggcaagggtc ccggccggtc cggtaaacac gatcgaccgt
      961 ggcttcgcgg tggccgaggc actcgggctc gacccgatcg acgagatcga cggggttcgc
     1021 acacccgcct caccgatccg gctggggcgc accccggccg aaacgcgact cccgcccccg
     1081 gccgagaacg ctcactcgga ggaaatccgc gactggcttc gaagttgatg gcagggatgg
     1141 aggaaaacga ggagaaagca ccccggccgg ttgccgtgtg ggtcggcggc accctggcgt
     1201 acgccgtcct ggtactcgca ctgggcctga tcctgaccgg catcaacccg gattcatcgg
     1261 tcttcccgcc gctcctgact gccctggccg gtctgttcgt ggccacggcg atcaacgtgc
     1321 gacccgcttc ctcgggtgac gcggatccgg actggaaccg cttccaggtc cacgtgatcg
     1381 tggtcgcgat caccagcatg gtcctcagca tcgggctggt catgtgtgcc ctcgccttcc
     1441 cgggcgagct gatcttccag ctggccttct ggggcgcggc gctggcgatc ctgatcggcg
     1501 cggcattcct gcttgccgtc gtctgggagc ggttgagcac ggaaggatct ccaccatccc
     1561 cggcctcgga accagaacca aagcccgacc ccgacccgga accggaaccc gaaccggaac
     1621 ccgagcccgc acctgaagca gagccggcgc cggagtccgg caaggtgaaa gccgcgaaga
     1681 aggaactgcg aagggcacca cagaccgatc tcgtcgccgc aacgctcaag aagtcaaaga
     1741 agacgaagaa gccggcaaag aagagcccga aggatccaaa ggcttcgaag gcaccgaaga
     1801 agccgaagaa gaaggactca aagaagaaga agtgagctcc tgccacactc ttggtgtgga
     1861 tctgacgctt cttgaagaaa cgatggcggg cctggagcag ccggcctacc ggaccggtca
     1921 ggtctgggag tggctggccc gcggggccgg ttcattcgac gagatgacga acctgcccgc
     1981 ctcgctgcgg caggaactcg aaggcaggct gccgatctcg accctggaag tcgacgcctc
     2041 gatgaagtcg gtcgatggca ccgagaaggc gctctttctg accgccgacg gccgcccggt
     2101 cgaggcggta ctgatgcgct accgcgacgg ccgccgttca ctctgcctct ccagccagtc
     2161 gggctgcccg ctcacttgca ccttctgcgc caccggcacg atgaaattcg gccgcaacct
     2221 gaccgagtcg gagatcctcg accaggccct tcacttccgg cggatcgagc cggtcaacca
     2281 cgccgtcttc atgggcatgg gcgagccgat gatgaacctc gacaacgtgc tggccgtctg
     2341 cgaaaggctg ccggaagtcg ggatcgccaa ctcccacacc acggtttcaa ccgtcggctg
     2401 gcagcccggg atcgaacgca tgacgacgga aggcccggcg gtccgcctcg ccctctcgct
     2461 tcacgccccg aacgacaagc tccgctccga aatcatgcct gtcaacgacc gctacccgat
     2521 ggaggacgtg gtcgccgcct gccgcgagtg gcgccacacc cgcaagcgca aggtctttat
     2581 cgagtacctg atgctcgacg gggtaaacga ctcggtcgag ctcgcccacg aactggccga
     2641 cctgctcctt ccgaagaacg acttcaaggt caacctgatc ccctacaacc cgtcgggaac
     2701 cggctaccag ggctcacccc gcgaagcgat cgaccgcttc cgcgacaccc tgatgaaacg
     2761 cggaatccac gccaccgtcc gcctcacccg aggccgcgac atagacgccg cctgcggcca
     2821 actggcggcc aaggccgcag cctgaccgga ccaggttctt cccgccccga gcggagtccc
     2881 ttccttccat ccgtgaatgt gagaggaagg gacgggtttc tcgtggaggg cgggtcggac
     2941 cccgcccgac ttacttatgc ggttccggag gcgaagcgtt cgcggctgag ttcgaggact
     3001 tcgagggcgg actccagggg gtaccagccg tcgacttcca cgtgcttgcc ttcgggctgc
     3061 ttgtagaccg cgaagaagtg ggagatttca tccttgagct ggctcgagag ctggtcgagc
     3121 gactcgatga agttccagtt cgggtcgtcg gccgggacgc agaggatctt gtcgtcgagc
     3181 tcgccgtcgt cggtcatgcg gaaaaggccg agcgggatcg ccgggatgat gcagccgggg
     3241 aaggtcggtt cggaaacgca aaccatggcg tcgagcgggt cgccgtcggc gcccatcgtg
     3301 ttcggcacca tgccgtaatc gacggggtag accatcgagg agaagaggaa acggtcgagc
     3361 ttgatcgcgc ccagctcttc gtcgtattcg tacttgtttc gggagccctt cgggacttcc
     3421 accagaacgt tcaggatctc agtcatatcg ccgcgaatcc tatcttttaa ggagtcaggc
     3481 ggggtccgac ccgccctcca tggcacctcc ggtggcttta gccgcccaag agcatgtgat
     3541 gcctgggatc ctcggtcggg cgccagggtt catcccagtc gcagatgccc ttgaccaggc
     3601 gatcggcctc ctccgggccc cagctgccga cctcgtaggg gacgaccggg ggcggatcat
     3661 cgagcagcgg ctgcacgatc cgccaggtct cctcgaccat ggagaacttg gtgaagaggt
     3721 cgtagcggcc gtggatcgcg tcatcgagca ggcgctcgta gggctccggt tcggctcccg
     3781 gctccttctc gaagagcacg tcgaggtccg cttcctcgaa gtcctcgata ccggcggcct
     3841 tggcgacgaa ggccatgcgc gccccgggca ccgggtcgat ccggaccacc atctggttgc
     3901 gcggcggctt gtgggacttc attccgaccc cggtgtgggg cggcctcttg aagaccac
//
LOCUS       DAOQMF010000067         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_11759_length_3901_cov_3.241810, whole genome shotgun sequence.
ACCESSION   DAOQMF010000067 DAOQMF010000000
VERSION     DAOQMF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11759_length_3901_cov_3.241810"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PLV77_01835"
     CDS             complement(<1..1308)
                     /locus_tag="PLV77_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNC14619.1"
                     /translation="MAFSAAEASASPWIHAHRGGPLENGKAAQAENSSSGFAASIERG
                     FVLEADVKLTSDKVPVIIHDDTLDRTTTCTGPVSAKTLDEIESECALDVKGIGDAATN
                     LRAGYPGPTPRIPTLAAFLQVLKRTGAKANIEIKNLPTDNDWDPTFEFAEIVANTVKN
                     SGVPSSQLIIQSFLIDNLTTFKSIDPDPQTSYLTINLVNPIAISAARDNGIDWVSPEW
                     PIDQAFVSDAHHAGLQVVPWTVDDAAGVREATRLGVDALITNDPLMARTQVRKVAPPL
                     AAIPKAPNLKSCNATFARDTRRPALAMLGRKDARGGPRVFAMQFKQEARHIKTYASFR
                     KKIECMIRKWVVPYKAKGRPNLVAFNEDVGLMTLGTGSRGAAARAAFAKPSSVTSCTG
                     EAPPCRAIYSLMQVTAAYAGPDGEYTSRYSIPNPFTRGFVAAAD"
     gene            1593..1787
                     /locus_tag="PLV77_01840"
     CDS             1593..1787
                     /locus_tag="PLV77_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14620.1"
                     /translation="MSRSGKKVPSCEDCYFRQKMLCALDLEGPCGTFRESRPEGLVPP
                     RQPMLLLRPPRWADRQIGAS"
     gene            1887..2678
                     /locus_tag="PLV77_01845"
     CDS             1887..2678
                     /locus_tag="PLV77_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC14621.1"
                     /translation="MKLTVLGKSPAWQDADGACSGYLVTEGDTTLLLDCGNGVFGKLR
                     KFVDYVDVDAIVISHHHVDHIIDLIPYGFALKFAPRQQPVPVDRWPGTGNPARPKLFV
                     PPGATGFFNRVSDSWGTGNLLEDAFDTAEYDPEGRLEIGPISISFTEVPHYITTYAMD
                     LTGPDGSRITYGADCCPNPALVELASGTDLLIAESTLPRPERTGVRGHLTPAEAGEHA
                     KLAGAGELILTHISDELDPGWAVEQASSVYEGPVQVAYEGLQVEV"
     gene            2712..3179
                     /locus_tag="PLV77_01850"
     CDS             2712..3179
                     /locus_tag="PLV77_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNC14622.1"
                     /translation="MSTRDPFANFARMRREMDELLGGTWGRATYISRRSSGFSPNVDV
                     YYSGDPQRAVVKCDLAGIDLGSVGIEVSGRELTIVGERPVQETEGRVYQQVEIPTGPF
                     RYVVELQVDVDSERATATYDDGVLRVELPLKEEAEVTRRVPVDRSRKGRVIDG"
     gene            3172..>3901
                     /locus_tag="PLV77_01855"
     CDS             3172..>3901
                     /locus_tag="PLV77_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNC14623.1"
                     /translation="MAEPGESVVPVLEVVSGSDEEVEEALRGEQELPEAMPVLPLRDT
                     VAYPDTLAPLGVGQERSIKLVDDVLAGNRMLVMVASRDPEEDTPSPDQLHGVGVVGVV
                     TRMLKVPDGTIRVLVQGSQRVRLGDFVTEKPYLVARITEMPDVIEETPELEALTRNVQ
                     KTFSDIIEQIPYLPEELQLAVTNVEDPSALAHLIAGALRIPIEEKQELLELVDVEARL
                     RRLSEILARELEVINLGTRIQSQVQ"
BASE COUNT          667 a         1242 c         1308 g          684 t
ORIGIN      
        1 gtcggcggcg gcgacgaaac cgcgagtgaa cggattcggg atcgagtagc gcgaggtgta
       61 ttcgccgtcc ggtcccgcgt aggcggcggt gacctgcatc agcgaataga tcgcccggca
      121 gggcggcgcc tcaccggtgc aggaagtgac cgaagagggt ttggcgaaag cggcgcgggc
      181 cgctgctccc cgtgaaccgg tgccaagggt catcagaccg acgtcctcgt tgaaggcgac
      241 cagattcggc cggcccttcg ccttgtacgg cacgacccac ttcctgatca tgcattcgat
      301 cttcttgcgg aaactcgcgt aggtcttgat gtgccgggct tcctgcttga actgcatcgc
      361 gaacacgcgg gggccgcccc tggcgtcctt gcggcccagc atcgccaggg caggccggcg
      421 ggtgtcacgg gcgaaggtcg cgttgcatga cttgaggttc ggggccttcg ggatcgcggc
      481 cagcgggggt gcgaccttcc tgacctgggt ccgggccatc aggggatcgt tggtgatcaa
      541 cgcgtcgact ccgagcctgg tcgcttccct gaccccggcc gcgtcgtcaa ccgtccaggg
      601 aacgacctga aggccggcgt ggtgggcgtc ggaaacgaaa gcctggtcaa tcggccactc
      661 cggtgaaacc cagtcgatcc cgttgtccct ggcggcgctg atcgcgatcg ggttgaccag
      721 attgatcgtc agatatgaag tctggggatc ggggtcgatc gacttgaacg tggtcaggtt
      781 gtcgatcaaa aaggactgga tgatcaactg cgaggagggg accccggagt tcttcaccgt
      841 gttggcgacg atctccgcga actcgaaggt ggggtcccag tcgttgtcgg tggggaggtt
      901 cttgatctcg atgttggcct tggctcccgt tctcttcaaa acctggagaa atgccgccag
      961 ggtggggatg cggggagtcg gtcccgggta gccggctcgc aggttcgttg cggcgtcgcc
     1021 gatccccttc acatcgagcg cgcattcgga ctcgatttcg tcaagggtct tggccgaaac
     1081 ggggccggta caggtcgtgg tgcggtcgag ggtgtcgtcg tggatgatca ccggcacctt
     1141 gtccgaggtc agtttcacgt cggcttcgag gacgaagccc cgttcaatcg acgcggcgaa
     1201 gccactgctc gagttctcgg cctgggccgc cttgccgttt tcaagcgggc cgccgcggtg
     1261 agcgtggatc cacggtgaag ccgatgcctc ggccgcgctg aaagccatcg cgacaagcgc
     1321 catgacgaga atcgagaacc tgaacctcac ttgaaccccc tctgtctcca gtcgccccgc
     1381 gggcgcctct ccgtccgtga ggataaccgc ggcggcgtcc gggcgggcgg atcgcccggt
     1441 gcatcattct gtccggaccc tggttcgccc cctcgggccg ggtcggcccg ccgcaaaatg
     1501 cggggcttgt tgcagacctg tgaaacaaga aagctgcgat ttgcggaatc gctgccgacg
     1561 cgatccggcg gggcttgctt ggatggcccg cggtgtcccg ctcaggcaaa aaggtcccct
     1621 cttgcgaaga ctgctacttc cgccagaaga tgctctgcgc actggatctc gaagggccct
     1681 gcggcacctt ccgcgagagc cggccggaag ggctggttcc gccgcgccag ccgatgctgc
     1741 tgctgcgccc gccccgttgg gcagaccggc aaatcggcgc cagctaacca ggcggacggc
     1801 gacgaacatt cacggatggt tcaccgggct ccgctttcgg ttgtccggag tagcatccgc
     1861 ttgatgaacg gaatcgggtt cctgtcatga agctgaccgt gctgggcaag tcgccggcgt
     1921 ggcaggacgc ggacggggca tgcagcggct acctggtcac cgagggcgat acgacgctgc
     1981 ttctcgactg tggcaacggg gtcttcggca agctgaggaa gttcgtcgat tacgtggatg
     2041 tcgatgcgat cgtcatttcg caccatcacg tggaccacat catcgatctg atcccctacg
     2101 gcttcgcctt gaagttcgct ccccggcagc aaccggtccc ggttgaccgg tggcccggga
     2161 ccgggaaccc ggcccggccg aaactgttcg ttccccccgg ggcgaccggt ttcttcaacc
     2221 gggtttcgga cagctggggt accggcaacc tgctcgagga cgcgttcgac acggccgaat
     2281 acgaccccga aggcaggctc gagatcggcc cgatatcgat cagcttcacc gaagtccctc
     2341 actacataac cacctacgcg atggacctga ccggcccgga tggttcccgc atcacctacg
     2401 gcgccgactg ctgccccaac ccggccctgg tcgagctggc atccggcacc gacctgctga
     2461 tcgccgagtc cacgctgccc cgtcccgagc ggaccggagt ccgcgggcat ctgaccccgg
     2521 ccgaagcggg tgaacatgcg aagctcgccg gggccgggga actcatcctc acccacatct
     2581 cggacgagct cgatcccggc tgggccgtcg aacaggcgag ttccgtctac gaagggcctg
     2641 ttcaggttgc ctacgagggt cttcaagtcg aagtttgagc cggaagtgct gcgttactaa
     2701 gcttcgaggt catgtcaacg cgcgacccat ttgccaactt tgcccggatg cgccgggaga
     2761 tggacgaact gctcgggggg acctggggcc gggccaccta cataagccgg cggtcaagcg
     2821 gcttttcccc caacgtcgac gtgtattaca gcggcgatcc ccagcgggcg gtggtcaagt
     2881 gcgacctggc cgggattgat ctgggctcgg tcgggatcga ggtcagcgga cgcgaactga
     2941 cgatcgtcgg cgagcggccg gtccaggaaa ccgaaggccg ggtgtatcaa caggtcgaga
     3001 taccgaccgg gcccttccgc tacgtggtcg aactccaggt cgacgtcgat tcggagcggg
     3061 ccaccgccac ctatgacgac ggggtgttgc gggttgaact gccactcaag gaagaggccg
     3121 aggtgacccg gcgggttccg gtcgaccgtt cccgcaaggg gcgcgtgatc gatggctgaa
     3181 ccgggcgaaa gcgtggtgcc ggtgctcgaa gtcgtcagcg gcagcgacga ggaagtggaa
     3241 gaggcgcttc gcggcgagca ggaactgccc gaggcgatgc cggtcctgcc cctgcgggac
     3301 acggttgcct atccggacac cctcgcgccg ctcggagtcg ggcaggaaag gtcgatcaag
     3361 ctggtcgacg atgtcctggc cggcaaccgg atgctggtga tggtcgcatc ccgcgatccc
     3421 gaagaggaca cgcccagtcc cgaccagctt cacggggtcg gggtggtcgg agtcgttacc
     3481 cggatgctca aggttccgga cgggacgatc cgggtcctgg tccagggcag ccagcgggtc
     3541 cggctgggtg acttcgtgac cgagaagccg tatctcgtcg cccggatcac cgaaatgccc
     3601 gatgtcatcg aggagacccc cgaactcgag gcattgaccc gcaacgtcca gaagaccttc
     3661 tccgacatca tcgagcagat cccctacctg cccgaggagc tgcagctcgc ggtcaccaac
     3721 gtcgaggacc cctccgcgct cgcccacctc atcgccggcg ccctgcgcat cccgatcgag
     3781 gagaagcagg agctgctcga gctcgtcgac gtcgaggccc gcctgcggcg cctctccgag
     3841 atcctcgccc gcgagctcga ggtcatcaac ctcggcaccc ggatccagtc gcaggtccag
     3901 g
//
LOCUS       DAOQMF010000068         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12053_length_3851_cov_3.481296, whole genome shotgun sequence.
ACCESSION   DAOQMF010000068 DAOQMF010000000
VERSION     DAOQMF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12053_length_3851_cov_3.481296"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLV77_01860"
     CDS             <1..437
                     /locus_tag="PLV77_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14624.1"
                     /translation="IIQWASLKWGIDTDTMRAAAVVESWWDMDVRGDNGDSFGLYQVR
                     RPYHCWGKCKIARYFTAFNADYYGAIIRSYYDGKQTWLNTVSGNGKKYRAGDLEGSMG
                     AWYSGRWWGPPERPIEPYLEKIARQKADRTWRHADFIAYRQP"
     gene            complement(499..2556)
                     /locus_tag="PLV77_01865"
     CDS             complement(499..2556)
                     /locus_tag="PLV77_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC14625.1"
                     /translation="MGALATFAFACLLMASPSLASAGWTSPATVSGPGTSVSQTTVAG
                     GSDGTSWVVWKRDVGGFNLIQGTRVQIDGTQGPIVTLSPVGQNANNPVIASRADGSAV
                     VGWTNESGTSDFIQSVSIAADGTVAAVTDRSVNGPAGQDASDLAIAVGADGTAGLAWL
                     RFDGTEPVVQAVRVASDGSSGTVHNLSAASAAPGPPDVGAAPPTTAGDPYFYRVMWPQ
                     GSAATSQVANADVMANDTVTTTVLLYTNASGADCADPFDIQIAYGSDGAMNAFWVCYK
                     KTIDFNTFNTFYNWSVQWLRTAKGVPLSTGGAASATNASPTIYLSPYPISGVVAAESF
                     TGQPAIAWIHDLDGGGQRVETWRIMINVLNGVPLPVGQGWANTTTEATAVGSPAIAVN
                     SNNTGIAGGVELGALPGQNTTNFTRFSNSSYDPVTPSGGFTYSEDPGYVLTDGGKTLA
                     AFTGITGPVGSAMVMTFEDPGISVDPDSFNFGHADIGTKRSVSISIRSTGQTTNTVTG
                     ITLGGANADRYAMSGADDCIREMTPNTNCRFTVTFTPGSTASQTAQISVTSDGGNEVA
                     NLTGTGLNRTRNSITAKPGHAARRKGKVVRIRVKASNLGGVSSNNTRICVNLRKRALK
                     LAGNRCRNLGTLGVGATRNLNYRIRVTWRAQRGVKLPVTFVLRSNNTVVRQAVVQLRR
                     KGR"
     gene            complement(2696..>3851)
                     /locus_tag="PLV77_01870"
     CDS             complement(2696..>3851)
                     /locus_tag="PLV77_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC14626.1"
                     /translation="PVGALRARYQRGSMNAVAEQERTIVIVEDDPNIADLLDMYLRDA
                     GFRVLQGPTGGRGLELIEQHRPAMVVLDIGLPDIDGFEVCRRVRARSSVPVLFLTARD
                     GEMDRIQGLELGADDYVTKPFSPREIVARVKAILRRGQPDERDRNDVVHVGDEYEVDT
                     TRREVRKGAEAVALATREFDLLAHLARNQGIGFTVSDVTGITYDDMKGRIMSELRKVF
                     RPEFLNRIDEVIVFHKLSREEVREIIELMLARVRNQVAEHELMLELDNGAKDFLADKG
                     WDPSMGARPLRRAIQRYIEDPLADEVLKAGGGDSVAGSLVTVTRDESGDDDDKPLKLK
                     ITKPRKPRKKKEDGQEKVGVGAGSTDSPSSEPPAVKPDVADGPAAESAAD"
BASE COUNT          623 a         1243 c         1300 g          685 t
ORIGIN      
        1 agatcatcca gtgggcctcc ctcaagtggg gcattgatac cgacacgatg cgcgccgccg
       61 cggtggtcga atcctggtgg gacatggacg tgcgcggcga caacggagat tcattcggcc
      121 tctatcaggt gcgccggccc taccactgct ggggcaaatg caagatcgcc cggtacttca
      181 ccgccttcaa cgccgactac tacggcgcga taatccgttc ctactacgac ggcaagcaga
      241 cctggctgaa caccgtttcc ggcaacggca agaagtaccg ggccggggat ctcgagggca
      301 gcatgggcgc ctggtactcg ggacgctggt ggggcccgcc ggaacgcccg atcgagccat
      361 acctcgagaa gatcgcccgg cagaaggccg accggacctg gcgccacgcg gacttcatcg
      421 cctaccggca gccctagggc ctaccggaaa tgaacctgcc acccgcggca ggttccgaat
      481 ccgccaggga gtttttgcct agcggccctt gcggcgaagc tgaaccacgg cctgccgcac
      541 cacggtgttg ttcgagcgca ggacgaaggt gacgggaagc ttgacgccac gctgggcgcg
      601 ccaggtcacc cggatccggt aattgagatt tctggtcgcc ccgaccccga gcgttcccag
      661 gttgcggcag cggttgccgg ccagcttcag agcccgcttg cggaggttca cgcaaatcct
      721 cgtgttgttc gacgataccc cgcccaggtt cgaggccttg accctgatcc ggacgacctt
      781 gcccttgcgt cgggcggcgt ggccgggctt tgccgtgatc gagttccggg tccggttgag
      841 gccggtgccg gtcaggttgg cgacctcgtt tccgccgtcc gaggtaaccg aaatctgtgc
      901 ggtctggctc gcggtcgaac cgggcgtgaa agtcaccgtg aagcggcaat tggtgttggg
      961 ggtcatttcc ctgatgcagt cgtcggctcc gctcatcgcg taacgatcgg cgttggctcc
     1021 gcccagggtg atcccggtga ccgtgttggt ggtctgcccc gtgctgcgga tcgagatcga
     1081 gacgctccgc ttcgtaccga tgtcggcatg gccgaagttg aagctgtccg gatccacgct
     1141 gatcccgggg tcttcgaagg tcatcaccat ggccgacccg accggaccgg tgattccggt
     1201 gaaggcggcc agtgtcttgc cgccgtcggt cagtacgtaa cccggatctt cgctgtaggt
     1261 gaagccaccg gagggcgtca ccgggtcata ggaactgttc gagaaccggg tgaaattggt
     1321 ggtgttctgc cccggcagcg ctcccagctc gacgccaccc gcgatgccgg tgttattcga
     1381 gttgacggcg atcgccgggg agccgaccgc ggtcgcttcg gtggtcgtgt tggcccagcc
     1441 ctggccgacc ggaagcggaa ccccgttcag cacgttgatc atgatccgcc aggtctcgac
     1501 ccgctggccg ccgccgtcaa ggtcgtggat ccaggcgatg gccggctgcc cggtgaagga
     1561 ctcggccgcg acgacgccac tgatcgggta cgggctcaga tagatggtcg gtgaggcgtt
     1621 ggtggctgaa gccgcccctc ccgtgctgag tggcaccccc ttggccgtac gcagccactg
     1681 gaccgaccag ttgtagaagg tgttgaaggt gttgaaatcg atcgtcttct tgtaacagac
     1741 ccagaaggcg ttcatggctc cgtcggagcc gtaggcgatc tgaatgtcga agggatcggc
     1801 acagtccgcc ccgctcgcat tcgtgtacag gaggacggtg gtggtgaccg tgtcattggc
     1861 catgacgtcg gcgttggcga cctggctggt cgccgccgag ccctggggcc acatgacccg
     1921 gtagaagtat gggtcgcccg ccgtggtcgg aggggcggcg ccgacatcgg gcggaccggg
     1981 cgcagccgag gcggcggaca ggttgtgaac cgtcccggaa ctgccgtcgg acgcgaccct
     2041 gacggcctgg acgaccggct cggtgccgtc aaagcggagc caggcaagac cggcggtgcc
     2101 gtcggcgccg acggcgatcg cgaggtcgct ggcatcctgg ccggccggcc cgttgaccga
     2161 ccggtcggtg accgccgcga cggtgccgtc ggcggcgatc gaaaccgact ggatgaaatc
     2221 ggaagtgccg ctctcgttgg tccatccgac caccgccgag ccgtcagccc ggctggcgat
     2281 aacgggattg ttggcgttct ggccgaccgg agagagggtg acgatcggac cctgggtgcc
     2341 atcgatctgg actcgggtgc cctggatcag gttgaaaccg cccacgtcac gcttccagac
     2401 gacccaggaa gtgccgtcgc tgccaccggc caccgtggtc tggctgacgc tggtcccggg
     2461 cccggaaacc gtggccggcg aagtccagcc ggcgctcgcg agcgaaggcg aagccatcag
     2521 caggcaggca aaggcgaaag tcgccagcgc gcccataatc gatccggttt ttgcacggcc
     2581 ataccgcaag cgaaccctcc gtcccagttg ctgtcgggtc agtctagctg taggaacacc
     2641 ggaaacaatc aagtgacgcg gtcggtgagg tgagaagcgg cggaagcgac gctgctcagt
     2701 cggcagccga ctcggcggcg ggaccgtcgg ccacgtcagg cttgaccgcc ggaggctcgc
     2761 tcgaggggga atcggtggaa ccggccccga caccgacctt ctcctgaccg tcttccttct
     2821 tcttgcgagg cttccgcggc ttggtgatct tcagcttgag cggcttgtcg tcatcgtcgc
     2881 ccgactcgtc gcgggttacc gtgaccagtg acccggccac cgaatcgccg ccgcccgcct
     2941 tcagcacctc gtcggccagc ggatcctcga tgtagcgctg gatcgcccgg cggagcggtc
     3001 gggcgcccat cgacgggtcc cagcccttgt cggcgaggaa gtccttggcg ccgttgtcga
     3061 gctcgagcat cagctcgtgc tcggccacct ggttcctgac ccttgccagc atcagctcga
     3121 tgatctcgcg cacttcctcg cgactcagct tgtggaagac gatcacttcg tcgatccggt
     3181 tgaggaactc cggccggaag accttccgga gctcgctcat gatgcgaccc ttcatgtcgt
     3241 cgtaggtgat gccggtgacg tcggagaccg tgaagccgat gccctggttg cgggcgaggt
     3301 gcgcgagcag gtcgaactcg cgggttgcga gagcgactgc ctcggcacct ttgcgtactt
     3361 cgcgacgggt ggtgtcgacc tcgtactcgt caccgacgtg caccacgtcg ttgcgatcgc
     3421 gctcgtcggg ctgaccgcgg cgcaggattg ctttcacgcg agcgacgatc tcgcgcgggc
     3481 tgaacggttt ggtgacgtag tcgtcggcgc ccagttccag cccctgaatg cggtccatct
     3541 caccgtcgcg ggcggtgagg aacaacactg gcaccgaact gcgcgctcga accctgcggc
     3601 agacctcgaa cccgtcgatg tcgggcagac cgatgtccag caccaccatc gccggtcggt
     3661 gctgctcgat gagctccagc ccgcggccac ctgtaggacc ttgcagcacg cggaacccgg
     3721 cgtcgcgcag gtacatgtcc agcaggtcgg cgatgttggg gtcgtcctcg acgatcacga
     3781 tcgtgcgttc ctgctcggcc acggcgttca tgctgccacg ctggtaccgt gcccgcagcg
     3841 caccgacggg g
//
LOCUS       DAOQMF010000069         3831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12157_length_3831_cov_3.022511, whole genome shotgun sequence.
ACCESSION   DAOQMF010000069 DAOQMF010000000
VERSION     DAOQMF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12157_length_3831_cov_3.022511"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLV77_01875"
     CDS             complement(<1..300)
                     /locus_tag="PLV77_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14627.1"
                     /translation="MTGPHPRLTALLVTGILLFPVAAGAKYQKTQPVGWGPRTSKAAA
                     KLVKRNGYEPRPINYRDNHRVPGEKLLRAWRKRSDMPYKRFVDGRFKGTTDEIIQW"
     gene            complement(297..848)
                     /locus_tag="PLV77_01880"
     CDS             complement(297..848)
                     /locus_tag="PLV77_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC14628.1"
                     /translation="MPEQSRKPRVSQEARRAEVIDAALIEFGRCGYAGTSTSMIANRA
                     GIRQPYIYALFENKRALFLACHEVLNDRIRETFRNAALPEDSPYERVRKMGLAYLGLL
                     HDDDRVRCHLQVFAAAGSDDLKEPIREGFNRLFEDVLEMSEASRPEVARFFATGMLLN
                     AMAALDEPFEMIRYLEVTPEDEL"
     gene            complement(962..2782)
                     /locus_tag="PLV77_01885"
     CDS             complement(962..2782)
                     /locus_tag="PLV77_01885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNC14629.1"
                     /translation="MSDSDTALAEVTWNLDDLLEGDHADPEAAVTALLDEAEKASEKF
                     AAEYEGKVSELDGPGLIVAMTKLADISDRAGRALNYAHLRFAADTADPANGALLQMGS
                     ERATRIQTQLLFFDLEWAALDDEVAEALLATPGLEFCEHHLRNERKYRDHLLSAPEEK
                     IVTELSVTGAGAWSRLFDELTSAIRVNPPGGEEPVSLDIALSGLHNPDREVRKATAEA
                     VTESLEPGLRTRAYVFNTLLQNKATMDRLREYPHWLATRNLSNEASDESVQALVQAVK
                     GRYDLARSWYRTKAKLLEVDRLADYDRMAAIATDDVTIEWEEGRQIVQSAFDSLAPQA
                     GEVVGRFFEDRWIDAPPAPSKRGGAFSASTVPSVHPYVMLNYTDRRRDVLTLAHELGH
                     GLHQALAAKQGIFHQDTPLTVAETASVFAEELVFGRLLAAEKDPASRLGLLSESVEGQ
                     IATIFRQIAMNQFEDAVHTARREEGELSVERFGELWAATQEDLLGDSVEVTDGYRSWW
                     SYVPHFIGSPGYVYAYAYGQLLALSVYRLYEEQGEAIVPGYLEMLTAGGSRSPEQLGA
                     LVGVDLSDPSFWDNGLDLVAGQIDAAQDAAEEVLRARSQG"
     gene            complement(2779..3120)
                     /locus_tag="PLV77_01890"
     CDS             complement(2779..3120)
                     /locus_tag="PLV77_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14630.1"
                     /translation="MSTSEDLSPQAPGGAREIRFLNIIAGVAILDFLLLIPLVWASRW
                     FADKHDIVSVLGPIHGFFFVVLIGLCAYGSLEKWWGWWFPILTLVTGGAIGSLIGDLV
                     VRRQLREKAAA"
     gene            complement(3227..3604)
                     /locus_tag="PLV77_01895"
     CDS             complement(3227..3604)
                     /locus_tag="PLV77_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14631.1"
                     /translation="MFWTVLLFLHVVAMAFFLGGQIMLAFTVVPVMRAEPAQPERIKQ
                     IAQRFGVGSLIALGVLLITGMMMASHFQLWDYGPFQVKMTLVIATIVAVLIHTLRGNS
                     HVLMGITFLLTLATVYAGVNLTH"
BASE COUNT          652 a         1200 c         1285 g          694 t
ORIGIN      
        1 ccactggatg atctcgtcgg tcgtgccctt gaagcggccg tcgacgaaac gcttgtacgg
       61 catgtcgctg cgcttgcgcc aggcgcggag cagcttttcg cctggtaccc ggtgattgtc
      121 ccggtagttg atcgggcgcg gctcgtagcc gttgcgcttg accagcttcg cggctgcctt
      181 gctggttcgc ggaccccacc cgaccggctg ggtcttctgg tatttcgctc cggccgcgac
      241 cgggaagagc aggatcccgg tgaccagcag ggccgtgagt ctggggtgag ggccggtcaa
      301 agttcgtcct ccggggtgac ttccagatag cggatcatct cgaacggttc gtccagggca
      361 gccatcgcgt tgagcagcat gccggtcgcg aagaagcggg ccacttcggg ccggctcgcc
      421 tcgctcatct cgagcacgtc ttcaaagagc cggttgaacc cttcccggat cggttccttc
      481 aggtcgtcgc tgccggccgc ggcaaagacc tggaggtggc agcggacccg gtcgtcatcg
      541 tgaagcaggc cgagataggc gagtcccatc ttccggactc gttcataggg actgtcctcg
      601 ggcagggccg cgttccggaa agtttcccgg atccggtcgt tgaggacttc gtgacaggcg
      661 agaaagaggg cccgcttgtt ctcgaacagg gcgtagatgt acggctggcg gattccggcc
      721 cggttggcaa tcatcgaggt tgaagtgccg gcgtagccgc agcgcccgaa ctcgatcagg
      781 gccgcgtcga tgacctcggc tcgccgagct tcctgtgaaa ccctcggttt tctcgattgc
      841 tctggcatcc ctggcccttc ggtcaaccgg gtaaccgtcc tttacccgga tccaatgttt
      901 tcgcaccctt cggccggtcc gccggaaaga gcgccggtgc aaaagacggg tcagatgtcg
      961 atcaaccctg gcttcgggcc cgaagcactt cttctgctgc gtcctgcgct gcgtcgatct
     1021 gtcctgccac cagatccaat ccgttgtccc agaacgaggg gtcactgaga tcaaccccga
     1081 ccagcgcccc gagttgctct ggggaacggg agccgccggc ggtcagcatc tccagataac
     1141 cgggcacgat cgcttcgccc tgttcctcgt agaggcggta gaccgagagc gcgagcagct
     1201 ggccgtaggc gtaggcgtag acgtaaccgg gggacccgat gaagtggggc acatacgacc
     1261 accaggagcg gtagccgtcg gtcacttcga cggaatctcc gagcagatcc tcctgggtcg
     1321 cggcccagag ctcgccgaac cgctcaaccg aaagctcgcc ttcctcgcgg cgggcggtgt
     1381 ggaccgcgtc ctcgaactgg ttcatggcga tctgccggaa gatcgtcgcg atctggcctt
     1441 cgaccgattc ggaaagcagg ccgagccggg aagccggatc cttctcggcc gccagcaacc
     1501 ggccgaagac cagttcctcg gcgaagaccg aagcagtttc ggccacggtc agcggcgtgt
     1561 cctggtggaa gatgccctgc tttgccgcca gcgcctggtg aaggccgtgg ccgagctcgt
     1621 gggccagcgt gagcacgtcc cggcgccggt cggtgtagtt cagcatcacg tagggatgaa
     1681 ccgaaggcac cgtcgaagcc gagaaggcgc cgcctcgctt gctcggggcc ggcggcgcgt
     1741 caatccagcg gtcctcgaag aagcgtccca cgacctcacc ggcctgcggg gcaagcgaat
     1801 cgaaggcgct ctggacgatc tggcggcctt cctcccactc gatcgtcacg tcatcggtgg
     1861 cgatcgccgc catgcggtcg tagtcggcga gacgatcgac ttccagcagt ttcgccttgg
     1921 tccggtacca ggaacgggcg aggtcgtagc ggcccttcac cgcctggacc agggcctgga
     1981 ccgattcgtc ggaagcttcg ttgctgaggt tgcgtgtggc cagccaatgc ggatactcac
     2041 gcagccggtc catggtcgcc ttgttctgaa gcagggtgtt gaagacgtag gcccgcgtcc
     2101 ttagcccggg ttccagcgac tcggtcaccg cctcggccgt cgccttgcga acttcccggt
     2161 cggggttgtg aagccccgaa agggcgatgt cgaggctgac cggttcctct ccgccgggcg
     2221 ggttgacccg gatcgccgag gtgagctcgt cgaagagccg ggaccaggct ccggcgcccg
     2281 tgaccgagag ttcggtcacg atcttttctt ccggggcgct cagcagatgg tcgcggtact
     2341 tgcgctcgtt tctcaggtgg tgctcgcaga actcgagacc cggtgtggcc agcagcgctt
     2401 cggccacctc gtcatcgagg gcggcccact cgaggtcgaa gaagagcagc tgggtctgga
     2461 tccgggtcgc ccgctccgag cccatctgga gcagggcccc gttggccgga tcggcggtgt
     2521 cggccgcgaa gcgcaggtgg gcgtagttga gcgcccggcc ggcccggtcg gagatgtcgg
     2581 ccagtttggt catggcgacg atcaggccgg gaccgtccag ctcggagacc ttgccttcgt
     2641 actcggctgc gaacttctcc gaagccttct ccgcttcgtc gagcagcgcc gtgaccgctg
     2701 cctccgggtc ggcgtggtcg ccttcgagca ggtcgtcgag gttccaggtg acttcggcca
     2761 gggcggtgtc ggaatcgctc atgctgccgc cttctccctg agctggcgcc gaacgaccag
     2821 gtcaccgatc agggagccga tcgctccgcc ggtgaccagg gtcaggatcg ggaaccacca
     2881 gccccaccac ttctcgagtg acccgtaggc gcagagcccg atcaggacca cgaagaagaa
     2941 gccgtggatc ggcccgagca ccgagacgat gtcgtgcttg tcggcgaacc agcgcgaggc
     3001 ccagaccagc gggatcagca gcaggaagtc gaggatcgcg accccggcga tgatgttgag
     3061 gaagcggatt tcgcgggcac cgccaggtgc ctgaggggaa aggtcttcag aggtgctcat
     3121 ggacccgtga agctatcaac ccgtcgatcc cgcccggttg tcaccgagaa ggcaaaagtg
     3181 gccacatagc gctcactttg gccttctcga tccggtccgg gggaaatcag tgggtcaggt
     3241 tcactccggc gtagaccgtc gcgagggtca gcaggaaggt gattcccatc agcacgtggc
     3301 tgttaccgcg cagggtgtgg atcaggaccg cgacgatggt ggcgatgacc agggtcatct
     3361 tgacctggaa aggcccgtag tcccagagct ggaaatggga ggccatcatc atgccggtga
     3421 tcagcagcac gccgagcgcg atcagggaac cgaccccgaa gcgctgcgcg atctgcttga
     3481 tccgctcggg ctgggccggt tcggcccgca tcaccggcac gacggtgaag gccagcatga
     3541 tctgcccgcc gaggaagaaa gccatggcga ctacgtggag gaacaacagg acggtccaga
     3601 acatggtccg aaggatattg atgcgtctcg ccggagaacc tgcggatttg ccgcagatgt
     3661 tgccttgagc tgagtttcgg cttgtcccgg ggggcgaaag cgtcctctcc cgtccttact
     3721 ctcatcaggt ggccaggatc cgttcacaac atgtctgtca gagctgcggt cacgtggcga
     3781 tggcctggac cggccaatgc ccggattgcc gggaatggaa cacgctggtc g
//
LOCUS       DAOQMF010000070         3822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12209_length_3822_cov_4.089727, whole genome shotgun sequence.
ACCESSION   DAOQMF010000070 DAOQMF010000000
VERSION     DAOQMF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12209_length_3822_cov_4.089727"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLV77_01900"
     CDS             <1..632
                     /locus_tag="PLV77_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNC14632.1"
                     /translation="APPGAARQSNRAALRMPAWIHRHFIPFGAKRKHQMARYSLRHYG
                     VRSWRLRNPRQIVEHVAVGNSVAGVYRTFAANRPDPEFHELPNVCAHFVVSGRGRVVQ
                     LVPLGIRCRHTVGLNHVSFGIEHTGFTDGDVLGNPRQMRASIRLTKWLRCRYGIGIRD
                     VIGHNESLGSRFHRELVPAMKNQTHGDFRHASMRRYRKALHDAGRCPRA"
     gene            739..2184
                     /locus_tag="PLV77_01905"
     CDS             739..2184
                     /locus_tag="PLV77_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNC14633.1"
                     /translation="MSTIAMTSLELARWQFGIVTLYHFIFVPLTIGLAFFTAWYQTRW
                     YRTKDEKWLRATRFWGKLMLISFALGVATGIVQEFQFGMNWSAYSRFVGDIFGAPLAM
                     EGLAAFFVESTFLGLWIFGWGRLSEKVHLACMWAVSLSTMLSAFFILAANSWMQHPVG
                     YELNQATNKVELKSIWDVLTNSTALYAMTHTMLAAALTGGMVIAGISAWHLLRKNEVE
                     VFSSSMKIALPSLAVAGLLTLVVGHFNGILMSEQQPMKMAAADAVFETEDGVGLSLFA
                     TGKFEGNPEETNRNIKLPNALSFIMTGKPDSEVKGVNNLNEEYRQRFGDPASPEYRGP
                     EFKTMDYAPIVWVAYWSWRVMLGCGFLLALFGVIGWWFNRKGKLPDSRRFLKLAMPAA
                     SLPFIAAAAGWIFTEMGRQPWVVFGLLRTQDANSPTVSATEVWISLVGMTLLYGVLAV
                     FAGKIFFKTAGKGPAEIEEGEEDKPYLGMAY"
     gene            2203..3306
                     /gene="cydB"
                     /locus_tag="PLV77_01910"
     CDS             2203..3306
                     /gene="cydB"
                     /locus_tag="PLV77_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNC14634.1"
                     /translation="MEDTTTLQFVWFVLISVLWIGYLVLEGFDFGIGMLMKLIGGSRE
                     EKRAILHTIGPVWDGNEVWLLVAGGATFAAFPEWYATLFSGFYIALLAILVALIVRNV
                     GFEMWGKRDTDAWRTGWEWCIAIGSLLPALLWGVAWANIIGGVPIEPVTTHGVESIEY
                     TGTFFDLLSVYTLLGGVASLAIFFAHGALFLEIRTEGDIREKARAVAAKATPVAALLA
                     AVFMAWTLIRQDSLEILSLIAAVVAVGSFGFAAINAKSQPMRAFTGTAVGIALYFIAQ
                     FIDLFPNAMVSSIDSAFDMSLNMASSTNYTLTVMTVVAGLLVPVVLLYQGWTYWVFRH
                     RLSAEGFGDVKSPMDLLDQKKEAGSGPESDQRA"
     gene            3327..>3822
                     /locus_tag="PLV77_01915"
     CDS             3327..>3822
                     /locus_tag="PLV77_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNC14635.1"
                     /translation="MATPSRAPGASRQARETQRRLARSSRVARNHLIVTVGLGFLQAV
                     LIIAQATLLAKVIAGVFMNGDSFAEVAPWLAWLAAISVGRGLAAAGFESAGRFGAARV
                     MSELREKLARHLLMNRPGALQAEQSGELVSSAIEGVAALENYFARYLPQMVLAGIVPV
                     AVLAW"
BASE COUNT          627 a         1220 c         1240 g          735 t
ORIGIN      
        1 ccgcgccacc gggcgcagcc cggcagtcga accgggccgc cctcaggatg ccggcctgga
       61 ttcaccggca cttcattccc ttcggcgcga agcgcaagca ccagatggcc cgctactcgc
      121 tgcgtcacta cggggtgcga tcctggcgcc tccggaatcc gcgccagatc gtcgaacacg
      181 ttgctgtggg caattcggtc gccggggtgt accggacttt tgccgcgaac cggcccgacc
      241 ccgaattcca cgagctgccc aacgtctgcg cccacttcgt ggtctcgggc cggggccgcg
      301 tggtccagct ggtcccgctc ggcatccgct gccgccacac ggtcggcctg aaccacgtct
      361 ccttcgggat cgagcacacc ggcttcaccg acggtgacgt gcttggcaac ccgcgacaga
      421 tgagagcctc gatccggctg accaaatggc tccgctgccg atacggcatc ggcatccggg
      481 acgtgatcgg gcacaacgag agtctcggtt cgaggttcca ccgtgaactg gtgccggcga
      541 tgaagaacca gacccacggc gacttccggc acgcgtcgat gcgccgctac cgcaaggcgc
      601 tccacgatgc cggtcgatgc ccccgggctt agccggggct aagccaaacc tccgtacttc
      661 cccgcagtaa ctgcggtgat tttccgcagg aatttcgacc tcctgcctag ctaatctggg
      721 atccatgaag gtggacgaat gagcaccatt gcaatgacca gtctcgagtt ggcccgctgg
      781 caattcggaa tagttacgct ctatcacttc atttttgtgc cgctcacgat cgggctggcc
      841 tttttcacgg cctggtacca gacccgctgg taccgcacga aggatgagaa gtggctgagg
      901 gcgacccgtt tctggggcaa gctgatgttg atctcgttcg cgcttggcgt cgcgacaggc
      961 atcgtgcagg agttccagtt cggcatgaac tggtctgcct actcgcgctt cgtgggcgac
     1021 atcttcgggg cgccactggc gatggagggc ctcgccgcct tcttcgtcga gtcgactttc
     1081 ctcggactct ggatcttcgg ctgggggagg ctctccgaga aggtccatct cgcctgcatg
     1141 tgggcggtct cgctttcgac catgctctcg gctttcttca tcctggccgc aaactcctgg
     1201 atgcagcacc cggtgggtta cgagctgaat caggccacca acaaggtcga gttgaaatcg
     1261 atctgggacg tgctgaccaa cagcaccgcg ctctacgcga tgacccacac catgctggcc
     1321 gcggccctga ccggtggcat ggtgatcgcc ggcatttccg cctggcacct gctccgcaag
     1381 aacgaggtcg aggtcttctc gagctcgatg aagatcgccc tgccttcgct cgcggttgcc
     1441 ggcctgctga ccctggtggt cgggcacttc aacggcatcc tgatgagcga gcagcagccg
     1501 atgaagatgg ccgccgccga tgccgtcttc gagaccgagg acggggtcgg cctgtcgctc
     1561 tttgccaccg gcaagttcga gggcaacccg gaagagacga accgcaacat caagttgccc
     1621 aacgccctct cgttcatcat gaccggaaag cccgactccg aggtcaaggg cgtgaacaac
     1681 ctgaacgagg agtacaggca gcggttcggt gatccggcca gccccgagta ccgcggtccc
     1741 gagttcaaga cgatggacta cgcgccgatc gtctgggtgg cctactggtc ctggcgcgtg
     1801 atgctgggct gcggcttcct gctggccctg ttcggcgtga tcggctggtg gttcaaccgc
     1861 aagggcaagc tgcccgactc gcgtcgtttc ctgaagctgg cgatgccggc cgcgtcgctg
     1921 ccgttcatcg ccgcggccgc cggctggatc ttcaccgaga tgggccggca accctgggtg
     1981 gtgttcggac tgctcagaac ccaggacgca aattcaccga cggtgagcgc taccgaggtc
     2041 tggatctcgc tggtcgggat gaccctgctc tacggcgtgc tggcggtgtt cgccggaaag
     2101 atcttcttca agacggccgg caaaggcccg gccgagattg aggaaggcga ggaagacaag
     2161 ccctacctgg gaatggccta ctaggggagc cggaggaaaa ccatggaaga cacaacaaca
     2221 cttcagttcg tctggttcgt cctgatctcg gtcctctgga tcgggtacct ggtcctcgag
     2281 ggtttcgact tcgggatcgg catgctgatg aagctgatcg gcggcagccg ggaagagaag
     2341 cgggcgatcc tgcatacgat cggcccggtc tgggacggca acgaggtctg gctgctggtc
     2401 gccggcgggg ccaccttcgc cgccttcccg gagtggtacg caaccctttt ctccggcttc
     2461 tacatcgcct tgctcgcgat tctggtcgcc ctgatcgtcc gcaacgtcgg tttcgagatg
     2521 tggggcaagc gggacacgga cgcctggcgc accggctggg aatggtgcat cgcgattggc
     2581 agcctgttgc cggccctgct ctggggcgtc gcctgggcca acatcatcgg cggcgtgccg
     2641 atcgaaccgg tgacgaccca cggtgtggag tcaatcgaat acacgggcac tttcttcgat
     2701 ctgctctccg tatacacgtt gctcggcggg gtcgcttccc tggcgatctt cttcgcccac
     2761 ggggccctgt tccttgagat caggaccgaa ggtgacatcc gggagaaggc gagggcggtg
     2821 gcggcgaagg caactccggt cgcggcgctg ctggccgcgg tcttcatggc ctggaccctg
     2881 atccgccagg actcgctcga gatcctgtcg ctgatcgccg cggtcgtcgc ggtcggcagt
     2941 ttcggtttcg cggcgatcaa cgcgaagtcg caaccgatgc gtgccttcac cggaaccgcg
     3001 gtcggcattg ccctctattt catcgcccag ttcatcgacc tgttcccgaa cgcgatggtt
     3061 tcaagtatcg attcggcctt cgacatgagc ctgaacatgg ccagctcaac caactacacg
     3121 ctgaccgtca tgacggtcgt cgcagggttg ctggtcccgg tggttctgct ctaccagggc
     3181 tggacctact gggtcttccg gcaccgtctt tcggccgaag ggttcggtga cgtgaagtca
     3241 ccgatggact tgcttgacca gaagaaggag gccggaagtg gcccggagtc ggaccagagg
     3301 gcgtagccgg ggccgagggg agagtattgg ccacgccctc tagggcaccg ggagcgagcc
     3361 gtcaggctcg tgaaacacag cgccggctgg cccggtccag ccgtgtagct cgcaatcacc
     3421 tgatcgtgac ggtcggcctg gggtttctcc aggccgtcct gatcatcgcc caggcgaccc
     3481 tgctggccaa ggtgatcgcc ggggtcttca tgaacgggga ctccttcgcc gaggtcgccc
     3541 cgtggctggc ctggctggcc gcgatctcgg tcggccgggg tctcgccgcg gccggatttg
     3601 aaagtgcggg ccgtttcggc gcggcccggg tcatgtccga gctccgggag aagctggccc
     3661 gccacctgct gatgaaccgg ccgggtgccc tccaggccga acagagcggt gaactcgtca
     3721 gctcggcgat cgaaggggtc gccgcgctgg agaactactt cgcccgctac ctgccgcaga
     3781 tggtgctggc cgggatcgtg cccgtagccg tgctggcctg gg
//
LOCUS       DAOQMF010000071         3806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12293_length_3806_cov_3.019728, whole genome shotgun sequence.
ACCESSION   DAOQMF010000071 DAOQMF010000000
VERSION     DAOQMF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12293_length_3806_cov_3.019728"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..537)
                     /locus_tag="PLV77_01920"
     CDS             complement(40..537)
                     /locus_tag="PLV77_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14636.1"
                     /translation="MIAAVPLSEWSDFFVASAGASAALAGLVFVAISINVARILQLDG
                     VPELGLVTLLLLIGVLVVSMFGLIPDQSEHAFGVELLVQSAFWTLVIAFLIRRSAAGL
                     GEGGGRLASRVILPVFGTLPYLAGAVLLITGSDIAMYWVFAGMLGAILAAVMNAWILL
                     VEILR"
     gene            complement(534..2204)
                     /locus_tag="PLV77_01925"
     CDS             complement(534..2204)
                     /locus_tag="PLV77_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14637.1"
                     /translation="MSLGRGFYRLLGSLVCAIAFFAGVGNASAELIDSEDLGDGVQRL
                     TYRIGPIEVTSGQNRIVYKMVTAEERPPVDGYITAFRPNLVNPDGSIPKSSSIMFHHG
                     VWINLSRRDATSGGMSERFIGTGEEKTEVVFPPGFGYFHKGTDVWLLNHMIHNLTPAE
                     HTLYITYQIDFVPLTSPKAEGMRGVRPIWMDVMNPNPYPVFDTYKGSGGKDGKIVWPY
                     EAKEDPYKDGIKRNLWTVDRDGVLVWSVGHVHTGGLATDLYLNRDGAKYAGPKCPKPR
                     ILRKKLKKNAPRSLRSARSKAAVKYRKCLARMPDVKGDRVHLFKSNAHYYEPAGPVSW
                     DMAMRNTRKDWLVQLKAGDTLEVTTTYETKRASWYENMGIAISYMADGTGGKDPYRTR
                     MDYTGPINHRHYSENNDHGGKLPVVGPDPTGLENGPFASDPFVISGYSYGQGDYRLPG
                     AAGRPPVVEKGDTFTFSLSDGDVGNKIWHSLTSCAAPCNKSTGIAYPIADGDFRFDSG
                     QMGVGGAPTVNRTTWTTPANLPVGTHTFFCRIHPLMRGAIRVVPKGSG"
     gene            complement(2230..3357)
                     /locus_tag="PLV77_01930"
     CDS             complement(2230..3357)
                     /locus_tag="PLV77_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC14638.1"
                     /translation="MSRRVLILGAGFGGLELGTMLSEELGDQVEVTLIDRNESFSFGY
                     SKLDIMFRGASRDSVSLPYSAVAKTGVTLLREEITAIDPETRTVTTDAGTHQGDALVI
                     ALGADYDLDSTPGLAERGHEFYSFAGAEAAAGPIADFDSGKAVIAVCGAPFKCPPAPS
                     ECALMLDDELTRRGVREQCEITLTMPLGTPVPPSPETSAALKAEFGQRGITFLGDMRP
                     KRVEPDRIIYQDGTELPFDLLLGVPKHRAPEVVIDSGITEDEYVPVDSATLKTRFEGV
                     YAVGDVATAGVPKAGVFAEGAAKVVARELVAEIRGEASPEPYDGRGSCYIEFGEDRIA
                     RVDIDFLSGPEKTGVFNAPSLDLRKEKAEFGSSRKSRWFNR"
     gene            complement(3389..>3806)
                     /locus_tag="PLV77_01935"
     CDS             complement(3389..>3806)
                     /locus_tag="PLV77_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC14639.1"
                     /translation="GSAQLWVRDQDEALDFWTNKVGFEVKVDVTMEELGGFRWLTVGP
                     VGDKGTSLVLMAIPPEPVMDADTRSQVEELMSKGFAGTVFLQTDNCQGAYEEMKARGV
                     EFTSTPEETPYGIDCGFTDPSGNSARLGEIKQEFVG"
BASE COUNT          646 a         1232 c         1235 g          693 t
ORIGIN      
        1 cgaggagccg gtccctttcg gggccggctc gtccgtgctc tagcggagga tctcgacgag
       61 caggatccag gcgttcatca ccgccgccag gatcgctccg agcatgccgg cgaaaaccca
      121 gtacatcgcg atgtcactgc ccgtgatcag caggaccgcg ccggccagat agggcagggt
      181 cccgaagacc ggcaggatga cccgggaggc caggcgaccg cctccttcac cgagaccggc
      241 cgcacttcgc ctgatcagga aggcgatgac gagcgtccag aatgccgact ggaccagcag
      301 ctcaaccccg aaggcatgtt ccgactggtc gggaatcagc ccgaacatcg agacgaccag
      361 caccccgatc aggagcagca gggtgaccag gccgagttcc ggtaccccgt caagctgcag
      421 gattcgcgcg acgttgatcg agatcgccac gaaaacgagg ccggccaggg ccgccgaggc
      481 cccggccgag gcgacgaaga agtcgctcca ttccgaaagc ggaacggcgg cgatcaaccg
      541 ctgcccttgg gcaccacccg gatcgcaccg cgcatcagcg ggtggatgcg acagaagaag
      601 gtgtgtgtgc cgaccggaag attggccggt gtggtccagg tcgtccggtt gaccgttggc
      661 gctccgccga cgcccatctg gccggagtcg aagcggaagt ccccgtcggc gatcggatag
      721 gcgatccccg tcgacttgtt gcagggtgcg gcgcaggaag tcagcgagtg ccagatcttg
      781 ttgccgacgt caccgtccga gagcgagaag gtgaaggtgt cacccttctc caccaccggc
      841 ggcctgccgg ccgcgccggg caggcggtag tcgccctggc cgtagctgta gccgctgatc
      901 acgaaggggt cgctcgcgaa cggcccgttt tcgagcccgg tcggatccgg cccgaccacc
      961 ggcagcttgc cgccgtggtc gttgttctcg gagtaatgcc tgtggttgat cggcccggtg
     1021 tagtccatcc gggtcctgta cgggtccttg ccgccggtgc cgtcagccat gtaggagatc
     1081 gcgatgccca tgttctcgta ccaggaggca cgcttcgttt cgtaggtcgt ggtgacttcc
     1141 agcgtgtcgc cggccttcaa ctggaccagc cagtccttgc gcgtgttgcg catcgccatg
     1201 tcccaggaaa caggtccggc cggctcgtag tagtgggcat tcgacttgaa gagatggacc
     1261 cggtcacctt tgacatcggg catcctggcc aggcacttgc ggtatttcac cgcggccttg
     1321 ctccgcgcgc tccgcagcga gcggggggcg ttcttcttca gcttcttcct gaggatccgc
     1381 ggcttcgggc acttcgggcc cgcgtacttc gcgccgtcgc ggttcagata aaggtcggtc
     1441 gcgaggccgc cggtgtgaac gtggccgacc gaccagacca gcacgccgtc ccggtccacg
     1501 gtccagaggt tgcgcttgat cccgtccttg tagggatcct ccttggcttc gtaaggccag
     1561 acgatcttgc cgtccttgcc acccgatccc ttgtaagtgt cgaagaccgg atagggattc
     1621 gggttcatca cgtccatcca gatcgggcgc accccgcgca tcccctcggc ctttggcgag
     1681 gtcagcggga cgaaatcgat ctggtaggtg atgtagagag tgtgttcggc cggcgtcagg
     1741 ttgtggatca tgtggttgag gagccagacg tcggtcccct tgtggaagta accgaagccg
     1801 ggcgggaaga cgacctcggt cttctcctcg ccggtgccga tgaaccgctc ggacattccg
     1861 cccgaagtgg cgtcgcgacg ggagaggttg atccagaccc cgtggtggaa catgatgctg
     1921 ctcgacttcg ggatggaccc gtccgggttg acaaggttcg gccggaaggc cgtgatgtac
     1981 ccgtcaaccg gcggccgctc ctcggcggtg accatcttgt agacgatccg gttctggccg
     2041 gaagtgacct cgatcggacc gatccggtag gtcaggcgct ggacgccgtc acccagatct
     2101 tccgaatcga tcagctccgc cgaggcgttg ccgaccccgg cgaagaacgc aattgcgcag
     2161 acgaggctgc ccagcagtct gtaaaaccct ctccccaagg acatggggaa agagtagcgc
     2221 cagtatcagt tatcggttga accagcgtga tttgcggctc gaaccgaact ctgccttctc
     2281 cttgcgaagg tcgagtgacg gtgcgttgaa gaccccggtc ttttccggcc cggagaggaa
     2341 atcgatgtca acgcgggcga tccggtcctc gccgaattcg atgtagcagg agcccctgcc
     2401 gtcgtagggt tccgggctgg cctcgccccg gatctccgcc acgagttcgc gggcgactac
     2461 cttcgccgcc ccctcggcga acactcccgc cttgggcacg ccggccgtgg cgacgtcccc
     2521 gaccgcatag accccttcga agcgggtctt cagggtggcg gagtcgaccg gcacgtattc
     2581 gtcttcggtg atgccggaat cgatcaccac ttccggggcc cggtgcttcg gcacgccgag
     2641 cagcaggtcg aagggaagct cggtcccgtc ctggtagatg atcctgtccg gttcgacccg
     2701 cttgggccgc atgtcaccga ggaaggtgat gccccgctgc ccgaactccg ccttgagggc
     2761 ggcggaggtt tccggcgagg gtggcaccgg tgtgccgagc ggcatggtca gggtgatctc
     2821 gcattgctcg cgaaccccgc gccgggtcag ttcgtcgtcc agcatcaggg cgcattcgct
     2881 cggggccggc gggcacttga aaggagcgcc acagaccgcg atcaccgcct tgccggaatc
     2941 gaagtcggcg atcgggccgg ccgcggcttc ggccccggcg aaggaataga actcgtgtcc
     3001 ccgttcggcc aggcccgggg ttgaatcgag gtcgtaatcg gcgcccagag cgatgaccag
     3061 ggcatctccc tgatgggttc cggcgtcggt tgtgaccgtg cgggtttccg ggtcgatcgc
     3121 cgtgatctct tccctgagga gggtgacccc ggttttggcc accgccgaat agggcaggct
     3181 gaccgagtcc cggctggccc cgcggaacat gatgtcgagc ttcgagtagc cgaaagagaa
     3241 cgactcgttt cgatcgatca gggtgacctc gacctgatcc cccagctctt cggagagcat
     3301 cgtgcccagc tcgagtccgc caaagcccgc tcccagaatc agtacccgtc tgctcacggg
     3361 gccagcatac tgccacccgg caaggccgtc agccgacgaa ctcctgcttg atctcgccga
     3421 gccgggcact gttcccggaa ggatcggtaa atccgcaatc gattccgtac ggcgtttcct
     3481 ctggcgtcga ggtgaactcg actccccggg ccttcatctc ttcgtaggcg ccctggcaat
     3541 tgtcggtctg gagaaagacc gtcccggcga acccctttga catcaactcc tcgacctggg
     3601 acctggtgtc cgcatccatc accggttcag gcgggatcgc catcaggacg agactggtgc
     3661 ctttgtcacc gaccggtccg acggtgagcc accggaagcc gccgagctct tccatcgtca
     3721 cgtcgacctt gacctcgaat ccgaccttgt tggtccagaa gtcgagcgct tcgtcctggt
     3781 ccctgaccca gagctgggcc gaaccg
//
LOCUS       DAOQMF010000072         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12535_length_3764_cov_3.077919, whole genome shotgun sequence.
ACCESSION   DAOQMF010000072 DAOQMF010000000
VERSION     DAOQMF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12535_length_3764_cov_3.077919"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLV77_01940"
     CDS             <1..184
                     /locus_tag="PLV77_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14640.1"
                     /translation="TGWIEPVAKVNPVTALLNGARSLMAGDPTGILLAYGIAFGMIAL
                     LALWALRGLRRAEVAG"
     gene            269..1408
                     /locus_tag="PLV77_01945"
     CDS             269..1408
                     /locus_tag="PLV77_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14641.1"
                     /translation="MSKLEIRRVQSKKDLKKFVKVPFLVHRDHPEWVPPLIMDRMDFL
                     NRDKNPYFDHAEVELWIAERDGEPVGRVSAQIDRNWDEYQGGNVGQFGFFETIDDQEV
                     ASALLDAGCEWLAGQGREKVYGPMDFTTNDEIGIQISGFDIQPSLLENCHQPYFQERV
                     EGAGFSKAMDLLMWHLEMGKLAKGLEFHPAIMESAQQSLDEHGITIRNMRKSDLANEM
                     GRFHEVYNEAWGDNWGFVPITREEVEFHAKTLKMVIDEDWAMIAETADGETVGAALTL
                     PDFNQVLAKMNGRIFPFGWYHFLTGRRKIDSVRILALGVKKAYQHTGVAAALYVKHIQ
                     TTDPNGVMKGEAGWILETNEPMNRALEGMGGEVTKKFRIYEKTLA"
     gene            1674..1985
                     /locus_tag="PLV77_01950"
     CDS             1674..1985
                     /locus_tag="PLV77_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14642.1"
                     /translation="MAEEVDGELISEEDVPAPQSGGEVRALPREYESGGALPLLKNEV
                     TTAAIAVAGGALAGAATVAAVRAVSGGSRRKARKLGRRARPANVVASRSFLVDVHVLG
                     R"
     gene            1996..3012
                     /locus_tag="PLV77_01955"
     CDS             1996..3012
                     /locus_tag="PLV77_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14643.1"
                     /translation="MIAERVVRPVAPLRMPRLVGGDLVFRLRGPWLERLLILSDEAMI
                     VRVREAARGEFVFAAEPVDPALLEGPGSPCLKEAGEPALEEGISRLRFSLGVDDDLAG
                     FRNAFREDPLLGRALNGRLQYRPMRRPVAWEALLWAITEQLIEYSRAARIQRQLIRRF
                     GVRLSPGVALGADDGGNRLPRSPRGRKQPSLMTVPTPAAIAGAAPAALEACGLSPRRS
                     IAMVKVAREVASGRVDPADPAGDKRLLAIPEIGPWTIACLALRGRGEPDALLAGDLSQ
                     VKLVGYLEGLGRLAEVEEVEEFYAPYAPWRGLAGEYIAMAYGSAVHGPGNRARIRQQL
                     RPAA"
     gene            complement(3095..>3764)
                     /locus_tag="PLV77_01960"
     CDS             complement(3095..>3764)
                     /locus_tag="PLV77_01960"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC14644.1"
                     /translation="IWLKKSKVKSVRDLKGKTVSTAGIPYQDAYLKTILSRAGLSEND
                     VKKVGVGQGLLPSIVSGRAQATLGPFWNIEGIELKKEGLKPTVSPVDKLGVPTYSELV
                     LVAKGSRIDEDPDPIRLFITALGRGTGFAVSHPNQATSTVLEANDALKPEVTAAQMKA
                     TLPLLGAPGGAKDAVPFGHQSTVKWQAFINWMVQQGVLANSQRAVDALTNELLPSKRI
                     DVDQ"
BASE COUNT          615 a         1236 c         1257 g          656 t
ORIGIN      
        1 gaccggctgg atcgagcccg tcgcgaaggt caatccggtg accgccctcc tcaacggcgc
       61 ccgcagcctg atggccggcg acccgaccgg gattctgctc gcctacggga tcgccttcgg
      121 aatgatcgcc ctgctggcgc tctgggctct gcgcggactg cgccgggccg aggttgccgg
      181 ctgagtccgt cgcgcggcgg caccgacctc ccggccgcca ggggcggcgc aaccggatcc
      241 cggggtccgg tccgctagtt tcccggcaat gtcgaagctt gagattcgcc gggttcagag
      301 caagaaggac ctgaagaagt tcgtcaaggt ccccttcctc gtccaccgcg accaccccga
      361 atgggtgccg ccgctgatca tggaccggat ggacttcctc aaccgtgaca agaacccgta
      421 tttcgatcac gccgaggtcg agctgtggat cgccgagcgc gacggtgagc cggtcgggcg
      481 ggtcagcgcc cagatcgatc gcaactggga tgaataccag ggcgggaacg tcgggcagtt
      541 cggcttcttc gagacgatcg acgaccagga ggtcgcttcg gccctgcttg acgcgggctg
      601 cgagtggctg gccggccagg gtcgggagaa ggtctacggg ccgatggact tcaccaccaa
      661 cgacgagatc ggcatccaga tttccggctt cgatatccag ccgtcgctgc tcgaaaactg
      721 ccatcagccc tatttccagg aaagggtcga gggtgcgggt ttcagcaagg cgatggacct
      781 gctgatgtgg catctcgaaa tgggcaagct cgccaaggga ctcgagttcc acccggcgat
      841 catggaatcc gcgcagcaga gtctcgacga gcacgggatc acgatccgca acatgcgcaa
      901 gtccgacctc gccaacgaga tgggacgctt ccacgaggtc tacaacgagg cctggggcga
      961 caactggggc ttcgtcccga tcacccgcga ggaagtcgag tttcacgcca agacgctcaa
     1021 gatggtgatc gacgaggact gggcaatgat cgccgaaacc gccgacgggg agaccgtcgg
     1081 cgccgctctc accctgcccg acttcaacca ggtcctggcg aagatgaacg gccggatctt
     1141 cccgttcggc tggtaccact tcctgaccgg tcgcaggaag atcgactcgg tccgcatcct
     1201 cgccctcggg gtcaagaagg cctatcagca caccggcgtc gccgctgccc tctacgtgaa
     1261 acacatccag accaccgatc ccaacggcgt gatgaagggc gaggcgggct ggatcctcga
     1321 aacgaacgag ccgatgaacc gggccctcga aggcatgggc ggcgaagtga ccaagaagtt
     1381 caggatctac gagaagacct tggcctagcc gcagacgccg cggagcgggt agccctccca
     1441 aagatggcgc taccgtcgcg ctcgtcccca cctgctcgct aatcggtgca gtaatcggga
     1501 agccactttg ggagggccac ccgccccacg gctacacgag tctcgcctca ggttcgcgag
     1561 atcccagttt gcgccacata ccggcacaaa gtgggcactc ggcggggttt gaatcgtcgg
     1621 tatttgcgga cttatgtcaa gtgaccgtcg atggcgactg tagattggcg gtcgtggctg
     1681 aagaggttga tggtgaactg atttccgagg aggacgttcc ggcgccgcag agcggtggcg
     1741 aagttcgcgc acttcctcgc gaatacgagt ctggcggggc tcttccactg ttgaagaacg
     1801 aggtaacgac cgcggccatc gccgtggccg gcggtgcact tgccggtgcc gctaccgtgg
     1861 cggccgtgcg ggccgtcagc ggcggctcca ggcgcaaggc ccgcaagctg ggccgccggg
     1921 cgcgcccggc caacgtggtc gccagtcgct cttttctggt tgacgtccac gtcctcggac
     1981 gataggggtg gtgcggtgat cgccgagcgc gtggtcaggc ccgttgcacc gctacgcatg
     2041 ccccgcctgg ttggcggtga cctggtcttc aggctgcgtg gcccctggct tgagcggctg
     2101 ctgatccttt ccgatgaagc gatgatcgtc cgggtccgcg aggcagcccg tggcgagttc
     2161 gtcttcgcgg ccgaaccggt cgatccggct ctgctcgaag gtccgggctc gccctgcctg
     2221 aaggaggcgg gggaaccggc gctcgaggag gggatctcca ggttgcgctt cagcctcgga
     2281 gttgacgacg acctggccgg gttccgcaac gcatttcgcg aggatccgct gctcggccgg
     2341 gccctgaacg gtcgtctcca gtaccggccg atgcggcggc cggtcgcctg ggaggccctg
     2401 ctttgggcaa tcaccgagca actgatcgag tacagccggg cggcccggat ccagcgccag
     2461 ctgatccgcc gcttcggggt tcgcctctcg cccggcgtgg cgctcggcgc cgatgacggc
     2521 ggaaaccgac ttccccgctc gcctcgcggc cggaaacagc cgagcctcat gaccgtgccc
     2581 acccccgcgg cgatcgccgg tgccgctccg gccgctctcg aggcctgcgg gctcagtcca
     2641 cggcgttcga tcgccatggt caaggttgcc cgcgaggtcg cctcgggccg ggtcgacccg
     2701 gcggacccag ccggggacaa gcgcctgctg gcgatcccgg agatcggccc ctggacgatc
     2761 gcctgcctgg ccctgcgtgg ccgcggtgag cccgacgcgc tgctggccgg cgacctcagc
     2821 caggtcaaac tggtcggtta cctcgaaggc ctgggccggc tggccgaggt cgaagaggtt
     2881 gaagagttct atgccccgta cgcaccctgg cggggcctgg ccggcgagta catcgccatg
     2941 gcctacggct cggcggttca cggcccgggc aaccgggccc ggatccgcca gcagctgcga
     3001 cccgccgcat aacggagtcc gttcatcaag tcgttgatgt cctgaacgga cgcacgggtt
     3061 tcattggcat tccggcgcag accggaatgc gtacttactg atccacgtct atccgcttgc
     3121 tgggcagaag ctcgttggtc agggcgtcga ccgcgcgctg cgagttggcc agcactccct
     3181 gctggaccat ccagttgatg aatgcctgcc acttcacggt gctctggtga ccgaacggaa
     3241 ccgcgtcctt cgcgcctccg ggcgcgccca gcagcggcag ggtcgccttc atctgggcgg
     3301 ccgtgacctc gggcttcagg gcgtcattgg cttcgagcac ggtgctggtt gcctggttgg
     3361 gatgggagac cgcaaaaccg gttccccggc cgagtgccgt gatgaagagg cggatcgggt
     3421 ccgggtcttc gtcgatccgg ctgcccttgg cgaccaggac cagctcgcta taggtcggga
     3481 cgccgagctt gtcgaccggg ctcacggtcg gcttgaggcc ttccttcttc agttcgattc
     3541 cctcgatgtt ccagaagggg ccgagcgtcg cctgggcccg gccgctgacg atcgacggca
     3601 gaaggccctg gccgaccccg accttcttca cgtcgttctc gctcagtccc gcccgcgaga
     3661 ggatcgtctt caggtaggcg tcctggtagg gaatcccggc ggttgaaaca gtcttgccct
     3721 tgaggtccct gacactcttc accttcgact tcttcagcca gatc
//
LOCUS       DAOQMF010000073         3724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12755_length_3724_cov_3.185337, whole genome shotgun sequence.
ACCESSION   DAOQMF010000073 DAOQMF010000000
VERSION     DAOQMF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12755_length_3724_cov_3.185337"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLV77_01965"
     CDS             <1..518
                     /locus_tag="PLV77_01965"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNC14645.1"
                     /translation="YVMPVHGDFQRIRLHSELAESVGVPGENIFRNHNGQALLLGPDG
                     AELTEETAGGVIYVDGVELSDPNDATLRDRRTISADGIVIVVATIAEDDGEVVSDPEV
                     IARGVPGLDEDAEFLDDLHDLIEDSLDRAAEAKVRDHDLVQQDLHDAVAGFIHKRLKR
                     RPMVVPVVVEV"
     gene            complement(1089..2612)
                     /locus_tag="PLV77_01970"
     CDS             complement(1089..2612)
                     /locus_tag="PLV77_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC14646.1"
                     /translation="MARAEGTLLDRMRRSRKWPPHWPIQWKLAAVSAGITFVILVAFG
                     LAVGQITTNQLRDNYAADTEAKANELAAEIRRRNLVAYLVTPGYIGDASGLSNLLASV
                     NGAADVTTLANKTRFQLPNTHDLGDPSTTSGISTYDGWQVATAVVYQSEDIPTPVALV
                     RFGRPMERLDASVGRIWISILAGALGATLLAALGGVILSRRAMRPISTLTSAAGQIAR
                     TRDPDVTLAEPEGDDEVAELTRTFNDMLHELSIARVEREQSLKRQREFVADASHELRT
                     PLTSVLANLELLDTSLRRPGSGNGDRDFEIESVESALRSSERMTRLVADLQLLAKADS
                     ERYRDLVPCDLSEIAANVAAELRPLSDNHTIALDTPEAAVVEGNGDDLHRVILNLIDN
                     AIRHTPAGSTITVTTRLDRDIAEIRVEDDGPGVPKEMWPTIFDRFVRHDGRGDRKKGK
                     GTGLGLAIVRVIARSHGGSARVGTSPEGGASFVVRIPAIKPAEKTPETASEKPQGNR"
     gene            complement(2614..3306)
                     /locus_tag="PLV77_01975"
     CDS             complement(2614..3306)
                     /locus_tag="PLV77_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC14647.1"
                     /translation="MVDEEQTGGRILVIEDDEEIVDVLRRTLRAEGYEVRATEDGGGG
                     LATASEFMPDLVVLDLGLPDMDGLEVCTRLREGSSELPILMLTARDGADNRVTGLDSG
                     ADDYLEKPYNREELLARIRALLRRHPPRGSAMLTVGDLKLNPDSQEAMRGDREVELTK
                     REFELLEYLMRNQRLVISRERLLEEVWGYDPLDETNTIDVFISNLRRKLEEGGEPRIL
                     HTKRGAGYVIKA"
     gene            3372..>3724
                     /locus_tag="PLV77_01980"
     CDS             3372..>3724
                     /locus_tag="PLV77_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNC14648.1"
                     /translation="MFVRSVRRGSDRRPSRGASARGAKAEKPAREPLRQHHLDLIGLG
                     CVLLGIYLVFVLYFGWNGGRLGSGISDGLKYLFGLGAYLAPVALFAAGAIAIFRPAIP
                     KTRPLRTGAVLLAAGL"
BASE COUNT          604 a         1173 c         1253 g          694 t
ORIGIN      
        1 agtacgtgat gccggttcac ggtgacttcc agcggatccg cctccactcg gagctggccg
       61 aatcggttgg cgtgccgggc gagaacatct tccgcaacca caacggccag gccttgctgc
      121 tcggcccgga cggcgcagag ctgaccgagg aaaccgccgg cggcgtgatc tacgtcgacg
      181 gcgtcgaact ttccgacccc aacgacgcca ccctgcgcga ccgccgcacg atctccgccg
      241 acggcatcgt gattgtggtc gccacgatcg ccgaggacga cggcgaggtc gtttccgacc
      301 cggaagtgat cgcccgcggc gtccccgggc tcgacgagga cgcggagttc ctcgacgacc
      361 tccacgacct gatcgaggat tcactcgacc gcgctgcgga agcgaaggta agggaccacg
      421 acctggtgca gcaggatctc cacgacgccg tcgccggctt catccacaag cgcctcaagc
      481 gccgcccgat ggtggtaccg gtagtagtcg aagtctgagg cccgcacggc gcctggagcg
      541 cggcgccggg aggtgccgca gaaaaagccc cgtcacgggg ctgaacgtgc ggcaccttcc
      601 tgctctctat ggagccgaat gaagcgtggc ctgaacccgc cgcttaaaac agagagaccg
      661 ctgggcctgg gggagggagt ggctcaacgg tctgctctga agaagcgagg ttgctggatg
      721 tctgggggag aatgcccaac aacctcttgt gtctcgtcca aaagcaacga tacggacgcc
      781 cagtaagaaa tccctaaacc cgaaggaagg atcttcttaa ggttcgtgga cctcgaccag
      841 agtccgatca tccatccttt tttcgtgatc ggacgggttg aactgggagg gcgggtcgga
      901 cccccgcccg acttgcagtc aagccgttaa acaacaagac cgccgggcct gggggaggga
      961 ggtggctcgg cggtctgtgt tgaagaagcg aagccgccgg ggcagtctgg gggagatccc
     1021 cggcggcttc atgtgtctcg tccgcgaaga aagatacgga gaggtcataa ataggcccta
     1081 aagcttcgtt aacggtttcc ttgaggtttt tcggaggctg tttctggggt tttttcggcc
     1141 ggtttgatgg ccggaatcct gaccacgaat gaggctccgc cctcgggtga agtgccgacc
     1201 cgggccgaac cgccgtggct tcgggcgatc accctgacga ttgccagtcc gagtccggtg
     1261 cctttcccct tcttgcggtc gccgcgtccg tcatgccgta cgaagcggtc gaatatcgtc
     1321 ggccacattt ccttcgggac gcccggaccg tcgtcctcga cccggatctc ggcgatatcg
     1381 cggtcgagcc tggtcgtcac cgtgatcgtg ctcccggcgg gggtatgccg gatcgcgttg
     1441 tcgatcaggt tgaggatgac ccggtggagg tcgtcaccgt tgccttcgac caccgccgcc
     1501 tccggcgtgt cgagcgcgat cgtgtggtta tcgctcagcg gccttagctc ggctgccacg
     1561 ttggcggcga tctccgaaag gtcgcagggc accaggtccc ggtatctctc cgagtcggcc
     1621 ttggcgagca gctggaggtc ggctacgagc cgggtcattc gctctgatga gcgcagcgcc
     1681 gattcgaccg attcgatctc gaaatcccgg tcgccgttgc ctgaacccgg ccggcggagc
     1741 gaagtgtcga gcagctccag gttggcgagc acgctggtca ggggagtgcg aagctcgtgt
     1801 gaggcgtcgg cgacgaattc gcgttgccgc ttcagcgatt gctcgcgttc gacccgggcg
     1861 atcgaaagct cgtgaagcat gtcgttgaag gtccgggtga gctcggccac ctcgtcgtcg
     1921 ccttccggct cggccagggt cacgtcgggg tcgcgggtgc gggcgatctg gccggccgcg
     1981 gaggtcagcg tggagatcgg gcgcatcgcc cggcgcgaga ggatcacgcc gccgagcgcc
     2041 gccagcagcg tggcacccag ggcaccggcc aggatcgata tccagatcct cccgaccgac
     2101 gcgtcgaggc gctccatcgg ccggccgaaa cggaccaggg cgaccggggt cggaatgtcc
     2161 tcggactgat agacgacggc ggtggcaacc tgccagccgt cgtaggtgga gatccccgaa
     2221 gtggtcgaag ggtcaccgag atcatgcgtg ttcggcagct ggaagcgggt cttgtttgcc
     2281 agcgtcgtca cgtcggccgc tccgttcacc gaggcgagca ggttgctgag cccgctggca
     2341 tcgccgatgt agccgggggt gaccaggtag gcgaccaggt tccggcgccg gatctcggcg
     2401 gccagttcat tcgccttggc ttcggtgtcg gcggcgtagt tgtcgcgcag ctggttggtg
     2461 gtgatctgcc cgaccgcgag gccgaaggcg accaggatga caaaggtgat tccggccgag
     2521 acggcagcca gtttccattg gatcggccaa tggggcggcc acttgcgcga ccgtcgcatt
     2581 cgatccagga gggtgccttc ggcccgggcc atctcaggcc ttgattacgt agccggctcc
     2641 gcgcttggtg tgaaggatgc ggggttcccc gccttcctcg agcttgcgac ggaggttgga
     2701 gatgaagacg tcaatcgtgt tcgtctcatc gagcgggtcg tatccccaga cctcttccag
     2761 cagacgttct cgcgagatga ccaggcgctg gttgcgcatc aggtactcga gcagttcgaa
     2821 ctcgcgtttg gtcagctcga cttcccggtc accgcgcatc gcttcctggg aatccgggtt
     2881 cagcttcagg tcgccgaccg tgagcatcgc cgagccacgg ggcggatgtc gcctcagcag
     2941 tgcccggatg cgggccagca gctcttcccg gttgtagggc ttctcgagat agtcgtcggc
     3001 gccgctgtct aacccggtga cccggttgtc ggcgccgtcc cgggcggtga gcatcaggat
     3061 cggcagctcg ctgctgcctt ccctgagccg ggtgcagacc tcgagcccgt ccatgtccgg
     3121 caggccaagg tccagcacga ccaggtccgg catgaattcg gaagcggtcg caaggccgcc
     3181 gccaccgtcc tcggtcgccc ggacttcgta tccctcggct ctcagcgtgc gcctgaggac
     3241 atcgacgatt tcctcgtcgt cctcgatgac gagtatgcgc ccgccggtct gctcctcatc
     3301 caccatggcc gggattctaa gcgaggtcga ttacaggaag ggtaaacgcg cgtttcgttg
     3361 aaaggaaagc catgtttgta cgttcagtcc ggcgcggttc agaccggcgc ccgtcgcgtg
     3421 gcgcctcggc gcgcggagcc aaagccgaga agcccgctcg tgagcccctg cgccagcatc
     3481 acctggacct gatcggactg ggctgtgtgc tgctcgggat ctacctcgtt ttcgttctct
     3541 acttcggctg gaacggcgga cggctcggtt cgggaatcag cgacgggctg aagtacctct
     3601 tcggtctcgg cgcgtacctg gctccggtcg cgctctttgc cgccggagcg atcgcgatct
     3661 tccgcccggc catcccgaag acccgtccgc tcaggaccgg ggcagtgctg cttgctgcgg
     3721 gcct
//
LOCUS       DAOQMF010000074         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12900_length_3697_cov_4.177924, whole genome shotgun sequence.
ACCESSION   DAOQMF010000074 DAOQMF010000000
VERSION     DAOQMF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12900_length_3697_cov_4.177924"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLV77_01985"
     CDS             complement(<1..228)
                     /locus_tag="PLV77_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14649.1"
                     /translation="MKKLSPIAFVPRRETVQSASLAPVALYLGAYLLVCFFSSDPLVL
                     VFATAGAALAGYGCGAGRAVNFSLKLGAFLAL"
     gene            complement(237..1148)
                     /locus_tag="PLV77_01990"
     CDS             complement(237..1148)
                     /locus_tag="PLV77_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HNC14650.1"
                     /translation="MAVALFFGLLLAGCGLGPGEDTGDASLLVTRDYGSRVLFDNPGL
                     AVNESSNAMRLLDGNAELETRYGGEYVQSVDGLSGDRKDGRSFDWFFAVNGIVAERGS
                     AQFPVTGGDRVWWDYRDWTDAMEVGAVVGSYPAPFSTGYDNRDWGVEIDCLGEETACR
                     MVENQLEGDGVTLKDRGENMRIVVGALPDLLEIPEMKRLNRGPSASGVFAGFGVGPVL
                     EKDGKPAPTLAVEGLRVDGRVGRKYGPDAGLVAALRRADDPPVWLVTGGTDQAVRMAA
                     AALNPGDLARRYAAVVNDGQVSSLPLP"
     gene            complement(1215..1940)
                     /locus_tag="PLV77_01995"
     CDS             complement(1215..1940)
                     /locus_tag="PLV77_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14651.1"
                     /translation="MTNRITSPALGLLLGLTLLLGLAAQPAAAGNRVAANLRVVTWKG
                     QIIFDGKVKTGTAKIKPNDDCLGGHAGPARTVTGPTALGLLYQASLRYKALQPLKISD
                     GDFGFGICGIADVSAKNKEWWVLRRNNKDSMSGAETTRVKAGDTILLYLARTYEDTAP
                     DSLVLNAPTKARKGARVKVRVFSFTGAGKRTPVEGARVFGAKGALTDAQGYTYLTING
                     RTNLVARQGSLIPSNRVSVSIRK"
     regulatory      complement(1988..2171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2226..2951
                     /locus_tag="PLV77_02000"
     CDS             2226..2951
                     /locus_tag="PLV77_02000"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNC14652.1"
                     /translation="MDFLSHVIDRLPVAVGPSWLDPESLIRTFGMLGVLAIVYMESGL
                     MVGFFLPGDSLLFTAGLLSANDVLPDIWVLLVTIPIAAIAGDQTGYWIGRKFGPPLFN
                     RPDSRFFKREYVDQTAAFFEKHGPRAIILARFVPIVRAFVPVMAGTSHMHYRTFLVYD
                     IVGGILWGAGVTTLGYFLGQIEFVKNNIEFILIGVVAVSVIPVIFEVRKARREAASHQ
                     KPEPFENPIDEEGEPVKHAESEV"
     gene            3014..>3697
                     /locus_tag="PLV77_02005"
     CDS             3014..>3697
                     /locus_tag="PLV77_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14653.1"
                     /translation="MREIKRCLAVVLALGAVFSVPAASAHQIPAGCLGPKPTVTFSAD
                     TLSQTAVPLRDGDQLTLAAHVDNKGESACNLSGLSIMVRPPSADEVSSDHFLLIDNVP
                     LGAGYDAEIINHGPYVVDLADDVFESQLELSWQATIHNGQADETVTGTGPGTKLTVTR
                     PQVELKVIPDVQSGPAPLTVTTVYLLTNTSPVPTAGTPAPGLVPTGSNGPRDVLADAG
                     CGPLNYLSGD"
BASE COUNT          638 a         1262 c         1146 g          651 t
ORIGIN      
        1 cagagcgagg aaggcaccga gcttcagcga gaagttcacc gcccggccgg ctccgcatcc
       61 gtaaccggcg agcgccgcgc cggccgtggc gaaaaccagt accagggggt cggaagaaaa
      121 gaagcagacc agcaggtagg cgccgaggta gagcgcaacc ggcgcgagcg aggccgactg
      181 aacggtttcc cgccggggca cgaaggcgat cggggagagc ttcttcatgg ccggcctcag
      241 ggtaagggca gggacgagac ctgtccgtcg tttacaaccg ccgcgtacct gcgggcaagg
      301 tcgcccgggt tgagcgccgc ggccgccatc cgaaccgcct ggtccgttcc gccggtgacc
      361 agccagacgg gcggatcgtc ggctcggcgc aatgcggcca cgaggccggc atcggggccg
      421 tacttccttc ccacgcggcc atcgaccctt agtccttcga ctgcaagggt cggagccggt
      481 ttgccatctt tctcgagaac gggtccgacg ccgaaaccgg cgaagactcc gctggcggag
      541 ggtccccggt tgagtctttt catttccggg atctcgagca gatcgggaag agcgccgacc
      601 acaatccgca tgttctctcc cctgtctttc agcgtgacac cgtccccttc gagctggttc
      661 tcgaccatcc ggcaggccgt ttcctcaccg aggcagtcga tctcgacgcc ccagtcccgg
      721 ttgtcgtagc cggtcgaaaa gggggccggg taggaaccga ccacagcccc gacctccatc
      781 gcgtcggtcc agtcgcggta atcccaccag acgcggtcgc cgccggtgac cgggaactgg
      841 gccgagccgc gttcggcaac gatgccgtta acggcgaaga accagtcgaa actgcggccg
      901 tccttccggt cgccggagag cccgtcgacc gactgaacgt actcgccgcc gtagcgggtc
      961 tcgagttcgg cgttgccgtc aaggagccgc attgcgttgc tcgactcgtt caccgccaga
     1021 ccgggattgt cgaacagcac ccgggacccg tagtcgcggg tgaccagcag gctggcgtca
     1081 cccgtgtcct cgcctggccc gagcccgcag ccggccagga gcagaccaaa aaacagtgca
     1141 accgccatgc cgacggcccg ggcaggaaag cccccggacc gtcggctttc ggttgcggac
     1201 ttcactcgtt ccgcttactt ccggatcgaa accgagacgc ggttcgacgg gatcagactg
     1261 ccctggcggg cgaccagatt cgtcctgccg ttgatggtca ggtaggtgta tccctgcgcg
     1321 tcggtcaacg ctccctttgc gccgaagacc ctggccccct cgaccggggt ccgcttgccg
     1381 gcaccggtga aggagaagac ccggaccttg accctggccc ctttccttgc cttggtggga
     1441 gcgttcagca ccagtgagtc cggggccgtg tcctcgtagg tcctggcgag gtagagcagg
     1501 atcgtgtcgc cggccttgac cctggtggtc tcggcaccgg acatcgagtc cttgttgttc
     1561 cggcggagaa cccaccactc cttgtttttg gccgagacat cagcgatacc gcagatgccg
     1621 aagccgaagt caccgtccga gatcttcagc ggctgaagcg ccttgtagcg gagcgatgcc
     1681 tggtagagca ggccaagcgc ggtcggaccg gtcacggtgc gagccgggcc ggcatggccg
     1741 ccgaggcaat cgtcgttcgg cttgatcttc gcggtgccgg tcttcacctt gccgtcgaag
     1801 atgatctgac ccttccaggt aactacccgg aggttggccg cgaccctgtt tccagccgcg
     1861 gcaggctgcg cagccaggcc aagcagcagg gtcagcccga gcagcaaccc gagggcaggt
     1921 gaggtaattc gattggtcat gcgttccttt tgtccggcgg ccctacgcga gaccgctttt
     1981 tcggtgcgcc atgtggcgca aagccggcag gtcttcggac ttggacgttt gctgggaggc
     2041 agccttccca gaccaatggt ccagtggctt tgtgccttca aggacgtccc taccgctgcg
     2101 cgtcagttcc ggactcggac cggattccct gaccccgctg aaggggtgcg accgacttcg
     2161 caggcgatga tagagactcc tgacgcggcg tgacctgtca agatgccgga ttccgcccct
     2221 cgcccatgga ctttctttct catgtcatag acaggttgcc ggttgccgtc gggccgagct
     2281 ggctcgaccc ggaatcgctg atccgcacct tcggcatgct cggcgtgctg gcgatcgtct
     2341 acatggaatc cgggctgatg gtcggcttct tcttgcccgg cgattccctg ctgttcacgg
     2401 cgggcctgct ctccgccaac gacgtgctcc cggacatctg ggtgctcctg gtcaccattc
     2461 cgatcgccgc gatcgccggt gaccagaccg gctactggat cggccgcaag ttcggaccgc
     2521 cgctcttcaa ccggcccgac agccgcttct tcaagcgcga gtacgtcgac cagaccgcgg
     2581 cattcttcga aaagcacggc ccgcgggcga tcatcctcgc ccggttcgtc ccgatcgtcc
     2641 gggccttcgt gccggtgatg gccggcacct cccacatgca ttaccgcacc ttcctggtct
     2701 acgacatcgt cggtggcatc ctctggggcg ccggcgtgac caccctgggc tatttcctcg
     2761 gccagatcga gttcgtcaag aacaacattg aattcatcct gatcggcgtg gtcgccgttt
     2821 cggtgatccc ggtcatcttc gaagtccgca aggccaggcg cgaagccgca tcccaccaga
     2881 agccggaacc gttcgagaac ccgatcgatg aagaaggcga accggtgaag cacgccgagt
     2941 ccgaagtctg aaccccgttt tcagccggcc gcggtcaggg tcggtactct tcaggcagaa
     3001 ggcaaggggt tcgatgaggg agataaagcg atgtctggcg gtcgttctgg cactcggcgc
     3061 agtgttttcc gtccctgcgg caagcgccca ccagattccc gcaggctgtc tcggccccaa
     3121 gccaaccgtc accttctcgg ctgacaccct cagccagacg gcagtgccac tcagggatgg
     3181 cgatcagctc accctcgcgg cccacgtcga caacaaaggt gagtcagcct gcaacctgag
     3241 cggcctctcg atcatggtcc ggccgccaag cgccgacgag gtctcgagcg atcacttcct
     3301 gctgatcgac aacgtgcccc tgggtgcagg ttatgacgcg gagatcatca accacggccc
     3361 ctatgtcgtc gatctcgccg acgacgtctt cgaatcgcag ctcgagctct cctggcaggc
     3421 cacgatccac aatggccagg cggacgaaac cgtgaccggc accggacccg gcaccaagct
     3481 caccgtgacc cggccccagg tcgagctcaa ggtgatcccc gacgtccagt ccggaccggc
     3541 cccgctcacc gtcaccaccg tctacctcct gaccaacacg agcccggtcc ccacggccgg
     3601 aacgccggcg ccgggacttg tgcccaccgg atcgaacgga ccacgagatg tcctggccga
     3661 cgccggttgc ggacccctca actacctttc cggcgac
//
LOCUS       DAOQMF010000075         3695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12911_length_3695_cov_3.577198, whole genome shotgun sequence.
ACCESSION   DAOQMF010000075 DAOQMF010000000
VERSION     DAOQMF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12911_length_3695_cov_3.577198"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLV77_02010"
     CDS             complement(<1..840)
                     /locus_tag="PLV77_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC14654.1"
                     /translation="MSDLLARLARSITGHWKTALLIAIASVIGIGILSGTAGEAPPEN
                     FDVPGTESQQAYDLFTEHSPALAGVDATVVFNATEGSVDQPARKQAIEESLKEIKGQP
                     DVLTVSNPVSEKAGAVSKDGKVAYVDVRYDLDFGDVTKVQSERLEEATDPVKSEGVEV
                     AMRGIVIDAGQQEQAPVGELIGVIIAIFLLTLLFRSISAMIATLLGALIGVMVGQMLL
                     VILAAPLGLPDFAATIAMMLGLGAGIDYSLLILGRYREQAAAGDSTRDAAAKSAATSG
                     SAVL"
     gene            935..1570
                     /locus_tag="PLV77_02015"
     CDS             935..1570
                     /locus_tag="PLV77_02015"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC14655.1"
                     /translation="MFTVAANSLRERKKAKTRETIVSEALRLFAEDGYSQTTIARIAE
                     QADVSPRTVATYFPAKEDIVFYLSDRTKERLIRGIEDRPEGVDTMTALRQWVLEEREL
                     FEEHHELMGCQQRVIENEESLRAHEQALMRDFETVLADGLAVDLGMEPTDLEPRLAAA
                     AAMAVFDLLGDDRKHRAEGELPPVEEQLAKLDQALTFIAGGVAALRDRQPG"
     gene            complement(1630..1815)
                     /locus_tag="PLV77_02020"
     CDS             complement(1630..1815)
                     /locus_tag="PLV77_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14656.1"
                     /translation="MNWRSSGKGRWISVYANSGHAYMIVAGLRFDTSMTPGNGPGWSK
                     SLRSTPGSFTVRHPAGF"
     regulatory      complement(2014..2159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2248..2955
                     /gene="rpiA"
                     /locus_tag="PLV77_02025"
     CDS             2248..2955
                     /gene="rpiA"
                     /locus_tag="PLV77_02025"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HNC14657.1"
                     /translation="MKPARHREVTFEPFEASSISNYEAKFKAAEEVAEMAQSGQVIGA
                     GSGSTAFLAVRAIASRVRHGELHDIRLIPTSIESELTITGLGLVQADLAEVAPDWLFD
                     GADEVDPDGNLIKGRGGALFREKLLFSACQLRRVLIDPSKRVDRLGTSFAVPVEVVPR
                     AASVATASLKSLGATEIVIRTGSGKDGPVLTEQGNLLVDCRFETIEDSLEDEINLITG
                     VLDSGLFQNYAPEIIEA"
     gene            complement(2989..>3695)
                     /locus_tag="PLV77_02030"
     CDS             complement(2989..>3695)
                     /locus_tag="PLV77_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14658.1"
                     /translation="TFWKSTAFTSTFEPVVYLLAFGLGLGSTLVADVDGIDYVQFVGT
                     GLVATAVVFSSALPAMFGTFVKERFQRTYDAILAAPVDVEELVTAEMLWIGLRAAVFG
                     CFPLIVTMFFGLEPRVTMLLVPIFAFVTALAFSAFGITMAALVSKIDQFNYVTTLVIT
                     PLFLVAGTFFPIDQLPQGFQIAAQFNPLYQLVQLVRDACFGMEAVDALRFLGLLTLAF
                     VLWRIAINRLTKRLIL"
BASE COUNT          744 a         1118 c         1244 g          589 t
ORIGIN      
        1 gagcaccgcc gagccggagg tggcagccga ttttgccgcg gcgtcccggg ttgaatcccc
       61 agccgccgcc tgttcgcggt agcggccgag gatcaagagc gaatagtcaa tgccggcgcc
      121 gaggccgagc atcattgcga tggtcgcggc aaaatcgggc aatccgagcg gggcggccag
      181 gatgaccagc agcatctgcc cgaccatgac cccgatcagg gcgccgagca gggtcgcgat
      241 catcgccgag atcgaacgga agagcagggt aagcaggaag atcgcgatga tcacgccgat
      301 cagctcgccg accggggcct gttcctgctg gccggcgtcg atcacgatgc cgcgcatcgc
      361 gacctcgacg ccttcgctct tgaccgggtc ggtggcttcc tcgaggcgct ccgactggac
      421 cttggtcaca tcgccgaagt caaggtcgta tcgcacgtcg acataggcga ccttgccgtc
      481 cttcgagacc gcgccggcct tctcggagac ggggttgctg accgtgagca cgtccggttg
      541 ccccttgatc tccttcagcg attcctcgat cgcctgcttt ctcgccggct ggtcaacgct
      601 gccttcggtc gcattgaaga cgaccgtggc gtcgaccccg gccagggccg ggctgtgctc
      661 ggtgaagaga tcgtatgcct gctggctttc ggtcccgggc acgtcgaagt tctcgggcgg
      721 cgcctcgccg gccgtgccgg agaggatgcc gatcccgatc accgaagcga tggcgatgag
      781 gagggcggtc ttccagtgac cggtaatcga acgggcaagg cgggcgagca gatcagacat
      841 tgagctccag aggtgagggg agggagagag cgggcaaatt atcacaacaa ctgcaaaact
      901 gcagtctatg cagaatcgct gctaaccttg gccggtgttc accgtcgccg caaacagcct
      961 gcgcgagcgc aagaaggcca agaccaggga gacgatcgtc tccgaggcgc tccggctgtt
     1021 tgccgaggat ggctacagcc agaccacgat tgcccggatc gccgagcagg ccgacgtctc
     1081 tccccgcacc gtcgcgacct atttcccggc caaggaagac atcgtcttct acctcagcga
     1141 ccggaccaag gaacggctga tccggggcat cgaagaccgt ccggagggag tcgacacgat
     1201 gaccgcgttg cgccagtggg tgctggaaga gcgtgagctc tttgaagaac atcacgaact
     1261 tatgggttgc cagcagcggg tgatcgaaaa cgaggaatcg ctgcgggccc acgaacaggc
     1321 tttgatgcgg gacttcgaga cggtgctggc cgacggtctc gcggttgacc tgggcatgga
     1381 gccgaccgac ctggaaccgc gcctcgccgc cgccgcggcc atggccgtct tcgacctgct
     1441 gggcgacgat cgaaagcacc gggccgaagg cgagctgcct ccggtggagg agcagctggc
     1501 aaagctcgac caggccctga ccttcattgc cggcggggtg gcggccctgc gcgaccggca
     1561 accgggctga aaacaaaacc gccccggcga ggggagccgg ggcggtcgtt acttccggga
     1621 gggatggtcc tagaaaccgg cgggatgccg cacggtgaag gagccggggg tggagcgcag
     1681 gctcttgctc cagccgggac cgttgcccgg ggtcatcgaa gtgtcaaacc gcagcccggc
     1741 cacgatcatg taggcatggc cggagttcgc gtagaccgag atccagcggc ccttgcccga
     1801 actgcgccag ttcatgaaac cggtcgaggc gagggggtag gcaacgaacc ggccgaaaga
     1861 ccgccgctgg aagaggccgc cttgcaggag gccgcggcgc cggcaggcgt gccaagagcg
     1921 aagatgagga agcaggcgag cagaccggta agcccgatcg cgacgctgcg aaccatggat
     1981 ctctgaatat gaggggttga ggggatgatg ccgatctcct ttttcagtgc ctgcggagtt
     2041 agctgacgga ctagcgcttt aggagtcgcg ctctcactcg gtcgagtggg ttcgccccaa
     2101 ctacctgggt cccccgcttc ccgggccttg cggcaacggg aattcggcaa atcggttttt
     2161 cagaatggtg aagtgtacag actttactaa tactttgcta acaaaccagc gcaaagccag
     2221 aagtttctat gccctaggct cttgatcgtg aaacctgccc gacaccgaga agtcacgttc
     2281 gaacccttcg aagcatcctc gatttccaat tacgaagcca aattcaaggc ggccgaagaa
     2341 gttgccgaaa tggcccagtc cggtcaggtg atcggggccg gctccggctc gaccgccttt
     2401 ctggcggtgc gggcgatcgc ctcgcgggtg cgccacgggg aacttcacga catccggctg
     2461 atcccgacct cgatcgagtc ggagctgacg atcaccggcc ttggcctggt tcaggccgat
     2521 ctggccgagg tggcgcccga ctggctgttc gacggggccg acgaggttga tccggacggc
     2581 aatctgatca agggtcgcgg cggcgcgctc tttcgggaaa agctgctctt ttcggcatgt
     2641 caactgcgcc gggtgctgat cgacccgtca aagcgggtgg accggctggg caccagcttc
     2701 gcggtgccgg tcgaagtcgt gccgcgggcc gcatccgtcg cgaccgcctc gctcaagtcg
     2761 ctcggagcca ccgagatcgt gatccggacc ggatccggca aggacggccc ggtcctgacc
     2821 gaacagggca atctcctggt cgactgccgg ttcgaaacga tcgaagacag tctcgaggat
     2881 gagatcaacc tgatcaccgg cgtcctcgac tccggcctct tccagaatta cgctcccgaa
     2941 ataatcgaag cctgagcccc tggccggcga agaccggccc gactgcagtc aaaggatgag
     3001 gcgtttggtg aggcggttga tcgcgattcg ccagaggacg aaggcgaggg tcagcaggcc
     3061 gaggaagcgg agggcgtcga ccgcctccat gccgaagcag gcatcgcgca ccaactgaac
     3121 gagctggtag agcgggttga actgggccgc gatctgaaaa ccctggggca actggtcgat
     3181 cgggaagaag gtgccggcaa ccagaaagag cggcgtgatg accagggtcg tgacgtagtt
     3241 gaactggtcg atcttcgaaa ccagggcggc catggttatc ccgaaggcgg aaaaggcgag
     3301 cgccgtgacg aaggcaaaaa tcgggaccag cagcatggtc acccggggct cgagcccgaa
     3361 gaacatcgtc acgatcagcg ggaagcagcc aaagaccgct gcccgcaggc cgatccagag
     3421 catctccgcg gtgaccagtt cctcgacgtc gaccggcgcc gcgaggatcg cgtcataggt
     3481 tcgctggaag cgctccttga cgaaggtgcc gaacatggcc ggcagggctg aagagaagac
     3541 gaccgcggtc gcgaccaggc cggtgccgac gaactggacg tagtcgatcc cgtcgacgtc
     3601 ggcaaccagg gtcgaaccga gaccaaggcc aaaggccagc aggtagacga ccggctcaaa
     3661 ggtgctggtg aaggcggtcg acttccagaa agtcc
//
LOCUS       DAOQMF010000076         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12974_length_3684_cov_4.296776, whole genome shotgun sequence.
ACCESSION   DAOQMF010000076 DAOQMF010000000
VERSION     DAOQMF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12974_length_3684_cov_4.296776"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLV77_02035"
     CDS             <1..526
                     /locus_tag="PLV77_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNC14659.1"
                     /translation="EVIVRQMLKKVEVETKGSTELLPGQLEDRQVLKAINKQVKADGG
                     TAAKAQEKILGITKASLATKSFLSAASFQETTKVLTDAALEGKRDRLVGLKENVIIGK
                     LIPAATGLKHYRSMTIEPTEPFAPAEEAVLLEEDLLVENGDGNGAGGADGFGESFADE
                     LDEISQEIDASTEK"
     gene            972..1904
                     /locus_tag="PLV77_02040"
     CDS             972..1904
                     /locus_tag="PLV77_02040"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC14660.1"
                     /translation="MFQLTKAGVSKDAVVARARSGWLTAIHDGVYATSPRQLTVWGRA
                     MAAVLAAGPDACLSHQSAAWLWKLDSARPVIHVVKRGGMNRELKPFDGYGKVIVHSTR
                     RLDPCEVTEVNGIPVTSFIRTTIDLAGGMTREALDDYLADADRENLLRLKELERELDR
                     WTGKKGITRLRRTLGDWHPSTEEEMLIFQKEFVGALVRAGAPRPRVDPLLDGCLIDLL
                     WEEYMLMFELDGHAFHRDPAMQTKDARRDRKHTLMGYQVNRFTRQEFRDNRAEVIGQA
                     LQLLRSRGWRGPDPQPLHQGVYRSLEAPGPSDHI"
     gene            complement(1895..3289)
                     /locus_tag="PLV77_02045"
     CDS             complement(1895..3289)
                     /locus_tag="PLV77_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNC14661.1"
                     /translation="MNEATGTAPGRERASRIAIVGGGASGTLVAANLLRMAEVPLEIE
                     LIDESGDSGPGIPYRTPNPEHRLNVPACRMSAWADDPDHYLNWRRSRDPEAAPEEYTA
                     RSIYGEYLQELLETAATKAPPFATLGRLTDRVAGISFADGRPVIRFPDRQLEVDQVVI
                     ATGPVPGGDPVPVPEALKDRGLYLPSAWDTEAVETAGKDEEVLIIGTGLTMVDVALTL
                     GAAPDGPRIHAISRSGMFPKTHLDRLTRLSTPQIPPEGPVSKRELLGAFAEQLSLASA
                     RGDDWRDAIDSMRLVTGDAWRRMSVEDRRWLLENVNRPWEVHRYRMAPSVGAAFHRLI
                     DSGRVEIQQARVGAIEESGSRVRVELVGPKRSESREYDRVISACGACTNIRRDAPEPV
                     RGLIDSGHLRPDDLLLGVEVDRDGAARDARGDRSATISVLGSLRRGVDWESIGVTELK
                     TQSRDIALRLLDQM"
     gene            3369..>3684
                     /locus_tag="PLV77_02050"
     CDS             3369..>3684
                     /locus_tag="PLV77_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNC14662.1"
                     /translation="MEASGVETGRQAEMNGAGPSGGGGPRVCVIGAGSSGITAMRSLQ
                     EKGIDFVCFEKGSWIGGNWKYDNDNGQSAAYKSLHINTSRQMMEYAAYPMPDELPDYP
                     DHF"
BASE COUNT          677 a         1146 c         1214 g          647 t
ORIGIN      
        1 cgaggtgatc gttcgccaga tgctgaagaa ggtcgaagtc gaaaccaagg gctcgaccga
       61 actcctgccc ggccagctcg aggaccgtca ggttctcaag gcgatcaaca agcaggtcaa
      121 ggccgacggc ggcaccgcgg cgaaggccca ggagaagatc ctcggcatca ccaaggcctc
      181 gctggcgacc aagtcgttcc tctcggcggc ctccttccag gagaccacca aggtcctgac
      241 cgacgccgcg ctggaaggca agcgcgaccg tctggtcggc ctcaaggaga acgtcatcat
      301 cggcaagctg atcccggctg cgaccggtct caagcactac cgctcgatga ccatcgagcc
      361 gaccgagccc ttcgctccgg cggaggaagc ggtcctgctc gaggaggatc tcctcgtcga
      421 gaacggcgat ggcaacggag cgggcggagc cgacggcttc ggcgaatcct tcgccgacga
      481 gctcgacgag atctcacagg agatcgacgc cagcaccgag aagtaatcac ggccgccggg
      541 aagccggcag ccaggagtca cgaagagggg cgccggccgg cgcccctttt cgttgcacga
      601 agcgcccgtt tcccattgcc cgagaggtgt cgatggattt cgtctatatg aacgaaatcc
      661 atcgacacct caacgccacg agcctcgccc gcgcgaggtg ggtatcggaa gtgtctatat
      721 gaacgaatct cataggcacc tggtggggcg ggtctggttg tgagtcgtgg aactgaattg
      781 gtcggaagtc gaggacaggg aggaggtcag tacttcgtga gccttcggtt gcacaatcgt
      841 tacctgggta acccactgtt gaggcaattc acggcactct gggggggatg gcgttagccg
      901 agccggccac atctcgtgag accaacttcc tcattgcccg ctattgcggt tctcaacatg
      961 gtgttgttgc catgttccaa ttgactaagg cgggcgtgtc aaaggacgca gtcgtcgcca
     1021 gggctcgttc cgggtggctg actgcgatcc acgatggggt gtacgcgacc agtccccgcc
     1081 agctgaccgt ctgggggagg gcgatggcgg cggttctggc ggctggaccg gacgcctgtc
     1141 tttctcacca gtcggcagcc tggctttgga agctcgattc tgcgcggccc gtcattcatg
     1201 tggtgaagcg aggtggaatg aaccgggaac tcaagccctt tgacggctac ggcaaggtga
     1261 tcgtgcacag cacgcggcga ctcgacccgt gtgaagtaac cgaggtgaac ggaatcccgg
     1321 tgacgagctt catccggacc acgatcgacc tggccggggg aatgacccgc gaggctcttg
     1381 acgactacct ggctgatgcc gatcgcgaga acttgctccg tttgaaggaa ctcgaacggg
     1441 agttggaccg ctggacgggc aagaagggaa tcactcggct acgcaggacc ctgggtgact
     1501 ggcatccctc cacggaggag gagatgctga tcttccagaa ggaattcgtg ggggcactgg
     1561 ttcgtgctgg tgcgccgcga ccccgggttg acccgttgct cgacgggtgt ctgatcgacc
     1621 ttctctggga ggagtacatg ctgatgttcg agctcgatgg gcatgccttc caccgggatc
     1681 cggccatgca gaccaaagat gccaggaggg accgcaaaca cacgctgatg ggttaccagg
     1741 tgaaccggtt cacgcgtcag gaatttcggg acaaccgggc cgaagtgatc gggcaggcgc
     1801 ttcagttgct gagaagccgc ggttggcgcg gcccggatcc gcagcccctt caccagggtg
     1861 tctaccgcag cctcgaagca cccggcccct cagatcacat ctgatcgagc agtcgcaggg
     1921 cgatgtcacg ggactgggtc ttgagttcgg tcaccccgat cgattcccag tcgacgccac
     1981 ggcgaagcga gcccagcacg ctgatcgtcg ccgagcgatc gccacgggca tcccgggccg
     2041 ccccgtcgcg gtctacttcg acgccgagca ggaggtcgtc gggacgcaga tggcccgaat
     2101 cgatcagccc cctgaccggc tccggggcat cccggcggat gttcgtgcag gctccgcagg
     2161 ccgagatcac ccggtcgtat tccctggact cgcttcgctt tgggccgacc agttcgaccc
     2221 tgacccgcga cccggattct tcgatcgcac cgaccctcgc ctgctggatc tccacccttc
     2281 ccgagtcgat cagccggtgg aacgcggccc ctacgctcgg agccatccgg tagcggtgga
     2341 cctcccacgg acggttcacg ttttccagca gccaccttcg atcttcaacg gacatccgtc
     2401 gccaggcgtc tccggtgacc agtcgcattg aatcgatcgc gtcgcgccag tcgtcgcccc
     2461 gggccgaggc cagcgacagt tgctcggcaa acgcccccag caattcgcgc ttcgacaccg
     2521 gcccctcggg cggtatctgc ggggtggaaa ggcgggtcag gcgatccagg tgggtcttgg
     2581 ggaacatgcc gctgcgggag atggcgtgaa tgcgaggtcc gtcgggtgcg gcgccgagag
     2641 tgagcgcgac gtcgaccatg gtcagcccgg tgccgatgat cagtacctct tcgtccttgc
     2701 cggctgtttc gaccgcctcg gtgtcccagg cactgggtag atagagcccc ctgtctttca
     2761 gcgcttcggg gaccggaacc gggtcgcccc cgggcaccgg cccggttgcg atgaccactt
     2821 gatccacctc gagctgccga tcggggaacc gaatgacggg ccttccgtca gcgaaggaga
     2881 tgccggcaac ccggtcggtc agccggccga gtgtggcgaa cggaggggct ttcgtcgcgg
     2941 cagtttcgag cagttcctgc aggtactcgc cgtatatgga tcgggccgtg tattcctccg
     3001 gggccgcctc gggatcgcgg gaccggcgcc agttcaggta atggtccgga tcgtctgccc
     3061 acgcgctcat ccggcaggcg ggcacgttga gacgatgctc ggggttgggg gtccggtagg
     3121 ggatgccagg accggaatcg ccggattcgt caatcagttc gatctccagc ggcacctcgg
     3181 ccattcgaag caggttcgcg gccaccagag ttccggaagc gccgcccccg acgatggcga
     3241 tccgggacgc ccgctcccgg ccgggtgcgg tcccggttgc ttcgttcatc cggactttcc
     3301 catcagtcag gagcctagtg agggcaaact gacacgaagc catgtcaagt attggctaca
     3361 ctgcccgaat ggaagcttca ggagtggaga cgggcagaca ggcggagatg aacggagccg
     3421 gcccgtccgg cgggggcggg ccgcgggtct gcgtgatcgg cgccgggtcc tcgggcatca
     3481 ccgcgatgcg aagccttcag gagaagggga ttgatttcgt ctgcttcgag aagggatcct
     3541 ggatcggcgg caactggaag tacgacaatg acaacggcca gtctgccgcc tacaagtcgc
     3601 tccacatcaa cacctcccgc cagatgatgg agtacgcggc ctacccgatg ccggatgaac
     3661 tcccggatta cccggaccat ttcc
//
LOCUS       DAOQMF010000077         3681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_12994_length_3681_cov_4.127965, whole genome shotgun sequence.
ACCESSION   DAOQMF010000077 DAOQMF010000000
VERSION     DAOQMF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12994_length_3681_cov_4.127965"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="PLV77_02055"
     CDS             <1..1296
                     /locus_tag="PLV77_02055"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNC14663.1"
                     /translation="PYRAGYTPAQRREIERKLVSGELLGVASTDALELGIDIGGLDAA
                     ICVTFPGTVAGLKQMWGRAGRRERGLAVYIAGPDGLDQYFCRHPDEFLERPVEAAILD
                     HLSPEIRDQHLIAAAYELPLTSEDEEFFGSGLEDAGQALVLRGELRKAGKGLIPRRAG
                     FAASDVSLRSSSAGDVAVIDRDSGEVIGTVETGRAFSTIHPGAVYMHLGASWEVERLD
                     PYEGEAIVHRFDEEWYTRPKTETEIFINRVLEEKEVCGVKLSFGEISVTDQVMAYQRI
                     LSADGEAFDLVDLDLPEQEFATRSLWYEVPEGFIGGMALNEMLGALHATEHGQIAVLP
                     LIAMCDRWDIGGLSTNLHMQTGTPTIFIYDGHPGGIGLTKRAFELFPRLVGDAHRLIA
                     ECPCDSGCPSCVQSPKCGNLNEPLFKAGSISLLEAMASA"
     gene            1339..2136
                     /locus_tag="PLV77_02060"
     CDS             1339..2136
                     /locus_tag="PLV77_02060"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNC14664.1"
                     /translation="MRTIEGSSGRIVVHEWIPVGEPTYVVLLAHGYAEHALRHAGLAE
                     KFEADGAAVYAPDHRGHGRSEGEPALVDEAQPMIDDLAAVYDLAREEHPGLPILLFGH
                     SMGGLLATLFIQEAERPVDAVVLSSPLLGGNPALVALLDLEEIPEIPIDPTVISRDEN
                     EQQAYLDDPMIYHGPFRRETLEGLVAGLDRARSGDGFGELPLLWIHGEGDMLVPYDLT
                     AETMEILSGPGTEAKSYPEARHELFHETNREDVYADLFDFVNRNLET"
     gene            complement(2236..2308)
                     /locus_tag="PLV77_02065"
     tRNA            complement(2236..2308)
                     /locus_tag="PLV77_02065"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2273..2275),aa:Phe,seq:gaa)
     gene            complement(2460..3353)
                     /locus_tag="PLV77_02070"
     CDS             complement(2460..3353)
                     /locus_tag="PLV77_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14665.1"
                     /translation="MPKNTGLDFPPVTVDLDVVQIQGEIALTKDGSGHFDEATGAMNM
                     DLSLSLTLGVDDLEALSQQVGIPLGTGALGCKISPLEVSFSTAKGWPHAGSSFTDKAT
                     PADGALAGAWRYKPAITAVEGDQSVCNIIGGFLKDVGGIWLGNSTTPLADMPAATDPM
                     PAKANCTELGKTGTFPNCVDPVCPEGQLGTYPDCHDPAPFAVSKVALSPTKANIKKNK
                     TVKLTLKVTNSGEVSGSATVSLKSSNKQVTLPKSIKVTVPAGKTASKTITVKAAKKAK
                     GKATITATASGKKGTATLTVK"
     gene            3463..>3681
                     /locus_tag="PLV77_02075"
     CDS             3463..>3681
                     /locus_tag="PLV77_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNC14666.1"
                     /translation="MSSSLSSGRVSPKATAGAAALTLGALGVVFGDIGTSPLYAFQTV
                     FQADHHAVKPNDTDVFGVISLVFWSITMI"
BASE COUNT          607 a         1200 c         1225 g          649 t
ORIGIN      
        1 ccctaccggg cgggctacac cccggcccag cggcgtgaaa tcgagcggaa gctcgtttcc
       61 ggcgagctgc tcggggtcgc ctcgaccgac gcgctggagc tcggcatcga catcggcggc
      121 ctcgacgccg cgatctgcgt cacctttccc ggcaccgtgg ccggcctgaa gcagatgtgg
      181 ggccgggccg gacgccgtga acgcgggctc gctgtctaca tcgccggccc ggacggcctg
      241 gaccagtact tctgccgcca cccggacgag ttcctcgagc ggccggtcga ggcggcgatc
      301 ctcgatcacc tctcacccga gatccgtgac cagcacctga tcgccgccgc ctacgaactg
      361 ccgctcacct ccgaggacga ggagttcttc ggctccggcc tggaggacgc cggccaggcc
      421 ctggtcctca ggggcgagtt gcgcaaggcc ggcaaggggc tgattccgag gcgggccggt
      481 ttcgccgcat ccgacgtttc gctccgctcc tcctcggccg gggacgtcgc ggtgatcgac
      541 cgtgattccg gcgaggtgat cggcacggtg gagaccggtc gcgccttcag caccatccat
      601 cccggcgcgg tctacatgca cctcggcgct tcctgggagg tggagcggct cgatccgtac
      661 gagggcgagg cgatcgtcca ccgtttcgat gaggagtggt acacccggcc gaagaccgaa
      721 accgagatct tcatcaaccg ggtgctcgag gagaaggaag tctgcggggt gaagctgagc
      781 ttcggcgaga tctcggtcac cgaccaggta atggcctacc agcgcatcct cagcgctgac
      841 ggcgaggcgt ttgacctggt tgatctcgac ctgcccgagc aggagttcgc gacccggagc
      901 ctctggtacg aggtacctga gggtttcatc ggcggcatgg ccctgaacga gatgctcggc
      961 gccctccacg cgaccgagca cggccagatc gcggtgctgc cgctgatcgc gatgtgtgac
     1021 cgctgggaca tcggcggtct ttcgaccaac ctccacatgc agaccggcac cccgaccatc
     1081 ttcatctatg acggccaccc cggcggcatc gggctgacca agcgcgcctt cgagctcttc
     1141 ccccgcctgg tcggcgatgc ccaccgcctg atcgcggagt gcccctgcga ttccggttgc
     1201 ccctcttgcg ttcagagccc caaatgcggg aacctgaacg agccactgtt caaggcgggc
     1261 tcgatcagcc tgctggaggc gatggcttcg gcctgattcg gccacccaga aggcaccagc
     1321 aaagtcaaag gagagcggat gcgcaccatc gagggaagca gcggcaggat cgtcgtccac
     1381 gagtggattc cggtcgggga gccgacatat gtggtgctgc tcgcccatgg ttacgccgaa
     1441 cacgcgctca ggcatgccgg cctggccgag aagttcgagg cggacggggc ggcggtctac
     1501 gcgccggatc atcgcggcca cgggcgctcg gagggcgagc cggccctggt cgatgaagcc
     1561 cagcccatga tcgacgacct cgccgcggtc tatgacctgg ctcgcgagga acaccccggc
     1621 ctgccgatcc tgctcttcgg ccattcgatg gggggtctgc tcgccaccct gttcatccag
     1681 gaggcggaac gcccggttga tgcggtcgtc ctttcgagcc cgctgctggg cggcaatccc
     1741 gcactggtcg ccctgctcga cctcgaggag atcccggaga tcccgatcga cccgacggtg
     1801 atcagccgtg acgagaacga gcagcaggcc tatctcgacg acccgatgat ctaccacggc
     1861 cccttccgtc gcgaaacgct ggaaggcctg gtcgccgggc tggaccgggc ccggagcggt
     1921 gacggtttcg gcgagttgcc gctgctctgg attcacggcg agggggacat gctcgtcccc
     1981 tacgacctga ccgccgaaac gatggagatc ctgagcggcc cgggcaccga agcgaagtca
     2041 tacccggaag cccgccacga gctattccac gaaaccaacc gcgaagacgt ctacgccgac
     2101 ctcttcgact tcgtcaaccg aaacctcgag acctagcagg gcagagcccg acccaacacc
     2161 attcatttct tccagtgggt cgggcgggtt gctcgtggag ggcgggtcgg accccgcccg
     2221 actactcgtg acatatggcg agggccggga tcgaaccggc gacaccacga ttttcagtcg
     2281 tgtgctctac caactgagct acctcgccaa ggaaggacag gctatcggga atcgccggtt
     2341 cagcgacgct tggcgcgggg ccgaaacggg tccgggaaac ccgggcaaag gaaagggccc
     2401 cggatcactc cggggccctc gcttcactcg gtccggcctg gccggaagaa gtgtcctcgc
     2461 tacttgactg tcagggtggc ggtgcccttc ttgccactgg cggtggcggt gatggtcgcc
     2521 ttgcccttgg ccttcttggc agccttgacc gtgatggtct tgctggccgt cttgccggcc
     2581 ggaacggtca ccttgatcga cttcggcagg gtgacctgct tgttgctcga cttcagtgaa
     2641 accgtcgcgc taccggaaac ctctccggag ttggtgacct tgagggtcag cttgacggtc
     2701 ttgttcttct tgatattggc cttggtcggc gaaagcgcga ccttgctcac cgcgaaggga
     2761 gccggatcgt ggcagtcggg gtaggtcccc agctggcctt cggggcagac cgggtcgaca
     2821 cagttcggga aggtacccgt cttgccgagc tcggtgcagt tggcctttgc cggcatcggg
     2881 tccgtcgcgg caggcatgtc ggcgagcggc gtggtgctgt tgccgagcca gatgccaccg
     2941 acgtccttga ggaagccacc gatgatgttg cagacggact ggtcgccttc aacagcggtg
     3001 atcgccggct tgtagcgcca ggcacctgcc agggcgccgt cggccggagt tgccttgtcg
     3061 gtgaagctgc tgcccgcatg cggccagccc ttcgcggtcg agaaggagac ttccagcggc
     3121 gagatcttgc agccgagcgc gccggtgccc agcgggatgc cgacctgctg cgagagagct
     3181 tcgaggtcgt caacgccgag ggtcagcgag aggctgagat ccatgttcat cgcaccggtg
     3241 gcctcgtcga aatgaccgga gccgtccttg gtcagggcga tttcaccctg gatctggacg
     3301 acgtcgaggt cgacggtgac cggcgggaag tccaggccgg tgttcttcgg cacggtgaag
     3361 aaaccggcaa catttcgcaa acaatcggtt tctggattgg cggttgcggg tagagggggc
     3421 gatcgagccg gaaaccggcg cgaggcgtag gctccccggc tgatgtcttc gtccctttcc
     3481 tcaggcaggg tttcaccgaa agccacggcc ggtgcggccg ccctcacgct gggggcgctc
     3541 ggcgttgtct tcggggacat cggcaccagc cccctttacg ccttccagac ggttttccag
     3601 gctgaccacc acgcggtcaa accgaacgac accgatgtct tcggcgtgat ctcgctggtc
     3661 ttctggtcga tcacgatgat c
//
LOCUS       DAOQMF010000078         3667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13080_length_3667_cov_3.286822, whole genome shotgun sequence.
ACCESSION   DAOQMF010000078 DAOQMF010000000
VERSION     DAOQMF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13080_length_3667_cov_3.286822"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="PLV77_02080"
     CDS             <1..1588
                     /locus_tag="PLV77_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC14667.1"
                     /translation="GSAPDSTPPLESFNPATGELVGSVETITPKQVQGIVDEVAEIQP
                     AWAELRPSDRATYLKRAATVLLDRLDDIAALLSREQGKPISECYTMELIPSIDALRWC
                     AKEGPGILKDEKISYPQPFFKTKRSKFRYQPIGVVGVIAPWNYPWTIPMQEIAIALMC
                     GNGVVLKPASLTPLLGEAIQSVFEDAGLPKGLIRNVHGGGRIGDALTKSTAGKIFFTG
                     SVPVGYKVGEECAKLMKGCVLELGGKDPMIVLEDANVDFAISGAVWGGFANAGQTCAG
                     IERAYVRREVSEKFIEGVVAGASALKVGDPSEVDTEIGPMVSKDQYELVCELVDDAVK
                     SGATLRCGGPVEVEGFPDGNFIAPVVLTDVTHEMRIMKEEIFGPVLPIITVESDQEAL
                     ELANDSEFGLGSSVWTKDRQRGERIADRIEAGMVWINDHMFTHGACQCTWGGVKDSGL
                     GHSHSKFGFYECVEIKLVTYEPGLTRNFWWHPYDETLTQAMKSSASLLYGKGGQRIEA
                     LKSGAGPLLQVGKRITKRGS"
     gene            1621..2037
                     /locus_tag="PLV77_02085"
     CDS             1621..2037
                     /locus_tag="PLV77_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14668.1"
                     /translation="MLGGGWLLFGRGGEESDSSKSGATRQQPAPARESASEPAEGPKL
                     PPSHCPADSSNCVVASGRVIYVEANDPDGDGDAHFVTVSEQSVTMSGITIFDVRADLR
                     PDPLPGVGDLVGGAGPVFPGHAGQKQIQVEEFHVAK"
     gene            2024..2698
                     /locus_tag="PLV77_02090"
     CDS             2024..2698
                     /locus_tag="PLV77_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC14669.1"
                     /translation="MSQSEIAIRLRDLGRDYGDRPALAGVDLDLPAGASLAVLGPNGS
                     GKSTLLRILATLLRPSRGEVTVLDTPLPGEAWKLRGRIGYLGHEPLLYEDLTGRENLE
                     FQARLYGLEKQEAGRRIEADLEMVGMSRRADYRVSDYSAGMKQRIAICRAVLHRPSLL
                     LLDEPDSNLDPEGREAARSLIGRRDQLAWQGETAVTRVAVSHEPEKARAEADQVLELA
                     IGGVAP"
     gene            2695..3375
                     /locus_tag="PLV77_02095"
     CDS             2695..3375
                     /locus_tag="PLV77_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNC14670.1"
                     /translation="MTPFGQAFAAIVAKDLRTELRTMRSLPAMVLFAVTTFVIFRFGL
                     DRTALSGSLASGVIWTTLLFAAVLGINRIFLPEREEGGFDAFRLAPVDRPALFAAKAA
                     VLFLYLAVLEVIVVPVFAVFFLQEWSGLAPMIVVLLLTNLGFAALGTLISSMAVNSRA
                     RDLLVPLLLLPLVVPLMIAATGATTHLLDAGGPSYDKFGTWLLVLGLYDGIFCLVGYA
                     VFDFLLED"
     gene            3443..>3667
                     /locus_tag="PLV77_02100"
     CDS             3443..>3667
                     /locus_tag="PLV77_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14671.1"
                     /translation="MLLAGFGLVFFWVPMDAIEGFRQKIFYLHVPLAIVALCGFMAGG
                     VEGFRHLRSNDRRHDANSYVFIHISVIFGVA"
BASE COUNT          579 a         1209 c         1227 g          652 t
ORIGIN      
        1 cggatccgcg cccgattcga ctccgccgct ggagtccttc aatccagcca ccggcgaact
       61 cgtcggcagc gtcgagacca tcaccccgaa acaggtccag ggcatcgtcg acgaggtcgc
      121 cgagatccag ccagcctggg ccgagctccg accctctgat cgggccacct atctgaagcg
      181 tgccgcgacg gtcttgctgg accgcctcga cgatatcgcc gcattgctgt cccgggagca
      241 gggaaaaccg atctccgagt gctacacgat ggagctgatc ccttcgatcg acgctctgcg
      301 ctggtgcgcg aaggaaggcc cgggcatcct caaggacgag aagatctcct acccgcagcc
      361 cttcttcaag accaagcgtt cgaagttccg ctaccagccg atcggcgtgg tcggggtgat
      421 cgccccctgg aactacccct ggacgatccc gatgcaggag atcgcgatcg ccctgatgtg
      481 cggcaacggc gtggtgctga agccggccag cctgaccccg ctgctcggcg aggcgattca
      541 gtccgtcttc gaggacgccg ggctgcccaa gggcctgatc cgcaacgtcc acggcggcgg
      601 ccggatcggt gacgccctga ccaagtccac cgcgggcaag atcttcttca ccggctcggt
      661 cccggtcggc tacaaggtcg gcgaggaatg cgcgaagctg atgaagggct gcgtgctcga
      721 gctcggtggc aaggatccga tgatcgtgct tgaggatgcc aacgtcgact tcgcgatctc
      781 cggcgcggtc tggggcggtt tcgccaacgc cgggcagacc tgcgccggaa tcgagcgggc
      841 ctacgtgcgg cgcgaggtca gcgagaagtt catcgagggc gtggtcgccg gggcctcggc
      901 gctgaaggtc ggtgacccct ccgaggtgga caccgagatc ggcccgatgg tcagcaagga
      961 ccagtacgag ctggtctgcg agctggtcga cgacgcggtg aagagcggcg ccacgctgcg
     1021 ctgcggcgga ccggtcgagg tcgaaggttt cccggacggc aacttcatcg ccccggtggt
     1081 cctgaccgat gtcacgcacg agatgcggat catgaaggag gagatcttcg gcccggtgct
     1141 gccgatcatc accgtcgagt ccgatcagga agcgctcgaa ctggccaacg attcggagtt
     1201 cgggctgggc tcctcggtct ggaccaagga ccgccagcgc ggcgagcgga tcgccgaccg
     1261 gatcgaggcc ggcatggtct ggatcaacga ccacatgttc acccacggcg cctgccagtg
     1321 cacctggggc ggagtcaagg attccggcct cggccattcg cattcgaagt tcggcttcta
     1381 cgagtgcgtg gagatcaagc tggtcaccta cgagccgggc ctgacccgga acttctggtg
     1441 gcatccctat gacgagaccc tgacccaggc gatgaagtcc tcggccagcc tgctctacgg
     1501 caagggcggc cagcggatcg aagcactcaa gtccggtgcc gggcccctgc tgcaggtcgg
     1561 caagcggatc accaaacgag gctcctgaag ctcttcgcct tcatcgtcct ggtcgccgtc
     1621 gtgctcggcg gcggctggct gctgttcggt cgcggcgggg aggaatccga ctcttcgaaa
     1681 tcaggtgcga cccgtcagca gccggcccct gcccgggaat ccgcgagcga accggccgaa
     1741 ggaccgaagc tgccgccttc gcattgcccg gccgattcct cgaactgcgt ggtcgccagc
     1801 ggaagggtga tctatgtcga ggcgaatgac cccgacggtg acggcgacgc ccacttcgtc
     1861 acggtctccg aacagagcgt gaccatgtcc ggaatcacga tcttcgacgt ccgggctgac
     1921 ctcaggcccg accccctgcc cggggtcggt gacctggttg gcggagccgg cccggtcttt
     1981 cccggccacg ccggccagaa gcagatacag gtagaagagt tccatgtcgc aaagtgaaat
     2041 tgcgatccgg ctgcgggatc ttggccggga ctacggggac cgccccgccc tggccggggt
     2101 cgatctcgat ctgccggccg gcgcctcgct ggccgtgctc ggaccgaacg gttccggcaa
     2161 gagcaccctg ctccgcatcc tggccaccct gctgcggccg agccgggggg aagttacggt
     2221 gcttgacacg ccgctgccgg gcgaggcctg gaagctgcgc gggcggatcg gctatctcgg
     2281 tcacgagccg ctgctttacg aagacctgac cggccgcgag aacctcgagt tccaggcccg
     2341 cctctacggg ctggagaagc aggaagccgg gcggcggatc gaggcggacc tggagatggt
     2401 cgggatgagc cgccgggcgg attaccgggt cagcgattac tcggccggga tgaagcagcg
     2461 gatcgcgatc tgccgggccg tgctccacag gccctcgctg ctgctgctcg acgagcccga
     2521 ctcgaacctg gaccccgagg gccgggaggc ggcaaggtcg ctgatcggtc gccgcgacca
     2581 gctcgcctgg cagggcgaga ccgcggtcac ccgggtggcg gtctcccacg agccggagaa
     2641 ggcccgggcg gaggccgacc aggtgctgga actggcgatc ggcggggtgg ccccatgact
     2701 cccttcggcc aggccttcgc cgcgatcgtc gccaaggacc tgcggaccga gctgcggacc
     2761 atgcgctcgc tgccggcgat ggtcctcttc gcggtcacca ccttcgtgat cttccgtttc
     2821 ggcctcgacc ggaccgccct ttcgggcagc ctcgcctccg gggtcatctg gacgaccctg
     2881 ctcttcgccg cggtgctcgg gatcaaccgg atcttcctgc cggagcggga agagggcggc
     2941 ttcgacgcat tccggcttgc cccggtggac cgcccggcgc tcttcgccgc caaggctgcc
     3001 gtgctcttcc tctacctggc ggtgctggaa gtgatcgtgg ttccggtctt cgcggtcttc
     3061 ttccttcagg aatggagcgg gcttgccccg atgatcgtcg tgctcctgct caccaacctc
     3121 ggttttgccg cgctcgggac cctgatctcg tcgatggcgg tcaattcgag ggcccgcgac
     3181 ctgctcgtgc cgttgcttct cctgcctctg gtggtgccgc tgatgatcgc cgcgaccgga
     3241 gccaccaccc acttgctgga cgcgggcggg ccgagctatg acaagttcgg aacatggctg
     3301 ctggtcctcg gtctatacga tgggatcttc tgccttgtcg gttatgccgt tttcgacttc
     3361 ctcctcgagg actgaatcga ccccgggcgg aacggatatg tcaggacgcg gtctaggaca
     3421 actttcgata gccacggcgc tcgtgctgct cgccggcttc gggctggtct ttttctgggt
     3481 gccgatggac gcgatcgaag gtttccgcca gaagatcttc tacctgcatg tgccgctggc
     3541 gatcgtcgcg ctctgcggct tcatggccgg cggagtcgag gggttccgtc atctgcgatc
     3601 gaacgaccgg cggcatgacg ccaactccta tgtcttcatt catatttccg tgatcttcgg
     3661 tgtcgcg
//
LOCUS       DAOQMF010000079         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13235_length_3641_cov_3.801450, whole genome shotgun sequence.
ACCESSION   DAOQMF010000079 DAOQMF010000000
VERSION     DAOQMF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_3641_cov_3.801450"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..2001)
                     /gene="uvrB"
                     /locus_tag="PLV77_02105"
     CDS             complement(4..2001)
                     /gene="uvrB"
                     /locus_tag="PLV77_02105"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNC14672.1"
                     /translation="MAEFKLNPAFKPLADQPAAIEQLTEGVEAGEKIQTLLGATGTGK
                     TFTMAGVIAETQRPALVMAHNKTLAAQLCNEFREFFPDNVVEYFVSYYDYYQPEAYVP
                     AQDLYIEKDSSINDEIDRLRHAATASLLARRDVIIVASVSCIYGIGSPALYQQQMQLF
                     KVGEWIDRDKVLRKLVRLQYERNDTVLSRGSFRVKGEVLEIMPSYAESAFRIMLFGDE
                     IEAIHHFDPLTGEVLDEVKHVSIWPATHYVTEEDSVERTLEAIRTELDQRLEELESEG
                     KELEAHRLRQRTLYDLEMIKETGFTSGIENYSRLFEGRAAGTPPHTLIDYFPEDFITF
                     VDESHQTIPQIGGMYLGDRSRKQTLVDFGFRLPSAIDNRPLKFDEFMDRMNQLVLVSA
                     TPGEFERTESTRVVEQIVRPTGIVDPEIDVRPTANQIDDLMNEIRATTDRGERTLVTT
                     LTKKMAEDLTNYLIEHGVKVRYLHSDIDTLERIQIIRELRLGEYDVLVGVNLLREGLD
                     MPEVSLVAILDADKEGFLRGETSLIQTIGRAARNTAGRVLMYGDKETEAMRSAISETD
                     RRRAIQVAYNERHGVTPTTIVKGVAEEGNLLAIEDRAPARRRRRSEDEFEGPDELEKS
                     IVQMEEEMHAAAEDLRFEQAARLRDEIRELRRDLDAMKAGA"
     gene            2035..2412
                     /locus_tag="PLV77_02110"
     CDS             2035..2412
                     /locus_tag="PLV77_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14673.1"
                     /translation="MRTLRRALGLAFIGVGILHFARPKFFLSIMPSWIPLHREAVAVS
                     GAAEIVGGVAMLPDRTASLAAPWLVALLVAVFPANVNMAIDQQQIEQAKASGIPLWAL
                     WARLPLQPLLMYLVWKSTRPQDG"
     gene            complement(2417..3355)
                     /locus_tag="PLV77_02115"
     CDS             complement(2417..3355)
                     /locus_tag="PLV77_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14674.1"
                     /translation="MTPDEPTDEMPGGPEDPTEVNSPGDEPTEVNPPGNEPTEVNPRG
                     DQASQVIPPGSRPAAAPDPGEQAGSRGPASRGQRGIGVLIGSIVASLVLVGAYALAGG
                     PVAPDGPADPCDARPWTDPQNLEEAAQQFALSAADGAACELGVSREELTRSIADDQAR
                     QDFMDEHGFSESDLEDALRSGLNRAVDDAENAGAISGITPALLRAAIKVMPMDTMIGL
                     LENADSIFNGDFGSMGGLGDALNAITGGFDGLGNSGDPGNSNDPLGSLGDALEGALPE
                     GTTDDLNQIIPEEERQKLEDQAREKLKQGLEDFLGP"
     gene            complement(3352..>3641)
                     /locus_tag="PLV77_02120"
     CDS             complement(3352..>3641)
                     /locus_tag="PLV77_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14675.1"
                     /translation="IVLDSPIDPSAKIDLGDKLANEVEAQGDRVPDLSPAFEPMPTDP
                     EDHARYEEILTGIESQLKDAATKAFSLSFLISALFGLLALIPIGMTRRLDL"
BASE COUNT          548 a         1178 c         1216 g          699 t
ORIGIN      
        1 ggctcaggcc ccggccttca tcgcgtcgag atcccggcgc agttcccgga tctcgtcacg
       61 cagacgggcg gcctgctcga agcggagatc ctcggcggcg gcatgcattt cctcttccat
      121 ctggacgatc gacttctcca gctcgtccgg gccctcgaac tcatcctcgc tgcgccgccg
      181 gcggcgggcc ggggccctgt cctcgatcgc cagcaggttg ccttcctcgg ccacgccctt
      241 gacgatcgtg gtcggggtca cgccatggcg ttcgttgtaa gcgacctgga tcgcccggcg
      301 gcggtcggtc tcggagatcg cggagcgcat cgcctcggtc tccttgtcgc cgtacatcag
      361 cacccggccg gcggtgttcc gggcggcccg gccgatcgtc tggatcaggc tcgtctcgcc
      421 gcgcaggaac ccttccttgt cggcgtcgag aatcgcgacc agcgagacct ccggcatgtc
      481 gaggccctca cgcagcaggt tgaccccgac cagcacgtcg tactccccca gcctgagttc
      541 gcggatgatc tggattcgct cgagagtgtc gatgtccgag tggagatagc gaaccttgac
      601 tccgtgctcg atcaggtagt tggtcaggtc ctcggccatc ttcttggtca gggtggtgac
      661 cagggtgcgt tcgccgcggt cggtcgtcgc gcggatctcg ttcatcaggt cgtcgatctg
      721 gttggcggtc ggccgcacgt cgatctcggg atcgacgatg ccggtcggcc ggacgatctg
      781 ctcgaccacc cgggtggatt cggtccgctc gaactcgccc ggggtcgccg aaaccagcac
      841 cagctggttc atcctgtcca tgaactcgtc gaacttgagc gggcggttgt cgatcgccga
      901 gggcagccgg aagccgaagt cgaccagggt ctgtttgcgg gagcggtcac cgaggtacat
      961 gccgccgatc tgggggatgg tctggtggga ctcgtcgacg aaggtgatga agtcctcggg
     1021 gaagtagtcg atcagggtat gcggcggggt gccggccgcg cgaccctcga acagccgcga
     1081 gtagttctcg atgccggagg tgaagccggt ttccttgatc atctcgaggt cgtagagggt
     1141 gcgctggcga aggcggtggg cctcgagttc cttgccctcg gattcgagct cctcgagacg
     1201 ctggtcaagc tcggtccgga ttgcctccag ggtgcgttcg accgagtcct cctcggtcac
     1261 gtagtgggtg gccggccaga tcgagacatg cttgacctcg tcgaggacct cgccggtgag
     1321 gggatcgaaa tggtggatcg cctcgatctc gtcgccgaag agcatgatcc ggaaggccga
     1381 ctcggcgtag gagggcatga tctccagcac ctcgcccttg acccggaagg agccgcggct
     1441 cagcaccgtg tcgtttcgtt cgtactgcag ccggaccagc ttgcgcagga ccttgtcgcg
     1501 gtcgatccac tcccccacct tgaacagctg catctgctgc tggtagagag ccggcgagcc
     1561 gatgccgtag atgcatgaga ccgaagcgac gatgatcacg tcccgccggg ccagcagcga
     1621 ggcggtcgcg gcgtgacgca ggcggtcgat ctcgtcgttt atcgaggagt ccttctcgat
     1681 gtagagatcc tgggccggga cataggcctc cggctggtag tagtcgtagt aggagacgaa
     1741 atactcgacc acgttgtcgg gaaagaactc gcggaactcg ttgcagagct gggcggcgag
     1801 ggtcttgttg tgagccatta ccagggccgg gcgctgggtc tcggcgatca ccccggccat
     1861 ggtgaaggtc ttgccggtcc cggtcgcgcc gagcagagtc tggatcttct cgccagcctc
     1921 aaccccttcg gtcaattgct cgatcgccgc aggctggtcg gccagcggct tgaaggctgg
     1981 attgagcttg aactcggcca cccgcccacc ataacgaccc gttaggttcg acccatgcga
     2041 actcttcgcc gcgccctggg cctcgctttc atcggagtcg gaatccttca ctttgcccgg
     2101 ccgaagttct tcctttcgat aatgccttcg tggatcccgc tccacaggga ggcggtcgcg
     2161 gtcagcgggg cggccgagat cgtcgggggt gtggcgatgc ttcccgaccg gaccgccagc
     2221 ctggcggcac cctggctcgt cgccctgctg gtggcggtct ttccggccaa tgtcaacatg
     2281 gcgatcgacc agcaacagat cgaacaggcg aaagcaagcg gcatccccct ctgggcgctc
     2341 tgggcccgac tgccgctcca gccgctgctc atgtacctgg tctggaagtc aacccgcccg
     2401 caggacggat gacggctcac ggcccgagga agtcctcgag gccctgcttg agcttctccc
     2461 gggcctgatc ctcgagcttc tggcgctctt cttccgggat gatctggttg aggtcgtcag
     2521 tggtgccctc cggcagggcg ccttcgaggg cgtccccgag gctgccgagc ggatcgtttg
     2581 aattgccggg gtcgccggag ttgccgaggc cgtcgaaacc gccggtgatc gcgttcagcg
     2641 catcaccgag tccgcccatc gaaccgaaat ccccgttgaa gatgctgtcc gcgttttcga
     2701 gcagtccgat catcgtgtcc atcggcatca ccttgatcgc ggcccgcagg agcgccgggg
     2761 tgatgccgct gatcgcgccg gcgttctcgg cgtcatcgac cgcccggttg aggccggaac
     2821 ggagcgcgtc ttcaaggtcg gattcgctga acccgtgctc gtccatgaag tcctgccggg
     2881 cctggtcgtc ggcgatcgag cgggtgagtt cttcccggct gactccgagt tcacaggctg
     2941 cgccgtcggc ggccgacagt gcgaattgct gggcggcttc ctcgagattc tggggatcgg
     3001 tccaggggcg ggcatcgcat ggatcagccg ggccgtccgg ggctaccggc ccaccggcca
     3061 gcgcgtaggc cccgaccaga accagcgaag cgacgatcga tccgatcagc accccgattc
     3121 cccgctggcc ccgactggcc ggaccgcgcg acccggcctg ttcacccgga tctggtgccg
     3181 ccgccggcct gctgccgggc ggaatcacct gtgacgcttg gtcgccgcgc gggttcacct
     3241 cggtcggctc gttccccggc gggttcacct cggtcggctc gtcgcccggc gaattcacct
     3301 cggtcggatc ctccgggccg cccggcattt cgtctgtcgg ttcgtccggg gtcaaaggtc
     3361 cagtctccgg gtcatgccga tcgggatcag ggccagcagc ccgaacagcg ccgagatcag
     3421 aaacgaaagc gagaaagcct tggtcgcagc atccttcagc tgggattcga tcccggtgag
     3481 aatctcctcg taccgggcgt ggtcctcggg atcggtcggc atcggttcga aggcaggaga
     3541 gagatccggc actcggtcgc cctgggcttc aacctcgttg gcgagcttgt ccccgagatc
     3601 gatcttggcc gagggatcga tcggggaatc gagcacgatc g
//
LOCUS       DAOQMF010000080         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13263_length_3637_cov_3.131491, whole genome shotgun sequence.
ACCESSION   DAOQMF010000080 DAOQMF010000000
VERSION     DAOQMF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13263_length_3637_cov_3.131491"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="PLV77_02125"
     CDS             <1..927
                     /locus_tag="PLV77_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNC14676.1"
                     /translation="NNKILPADVPGPLDPNISNTSGSGTIAVETSGSTATVAEADFNM
                     PIVWIPDPTAGGAPIPMKFGPKGDLTGTWDAGTGQLSLTGTLQVDVIWGFDEGGAKIC
                     RFTAPNTTWTTAPNAISPGVAFNGVGGLSGNGAISAFWDELPAGESINGGDCGKPNAV
                     VRDPGAVWLSSGIAEVPAPPTCQELGKDGLWPDCVDIDWPDPDPVVEITKVTVTKGTV
                     TAGQKAKIKVNVTNSGDLAATPITIKLASSSKQVKVPGQVKLNVPAGKTATASITVTA
                     TKNARGKATITASTSGKSGKAVVTVKKPRKRK"
     gene            complement(1026..1487)
                     /locus_tag="PLV77_02130"
     CDS             complement(1026..1487)
                     /locus_tag="PLV77_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC14677.1"
                     /translation="MIEIRPVAPGEFPLFEPLFVLYQEFYEVKDIDRGRNREFFSRFL
                     VGEDGVGSADGWLLGAFDGEEAVGFGCFYRHKSSLSATDVVLMNDLFVTEAARGGGTG
                     RALIEAGVELARESGASHLTWATAPDNHTAQRLYDSTGAHKSTWLEYEIEA"
     gene            1691..2989
                     /gene="clpX"
                     /locus_tag="PLV77_02135"
     CDS             1691..2989
                     /gene="clpX"
                     /locus_tag="PLV77_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNC14678.1"
                     /translation="MAGPTDTNEQLLCSFCGKSQRQVKKLIAGPGVYICDECIDLCNE
                     IIDEESVSAAAPAGLDLDRLPRPQEINSVLDEYVVGQETAKRALSVAVYNHYKRVRMA
                     EEGDGDVELQKSNIMLLGPTGCGKTLLAQTLARILNVPFAIADATALTEAGYVGEDVE
                     NILLKLIQAADYDVAKAETGIIYIDEIDKITRKADNPSLTRDVSGEGVQQALLKILEG
                     TTASVPPQGGRKHPHQEFLTIDTTNILFICGGSFSELEKVIERRVDHKGIGFGAAITE
                     QAHDDPGELFEQVLPEDLVEYGLIPEFIGRLPVVAALHQLDQTDLVRILTEPKHALVR
                     QFQRFFEFDDIELVFADDSLEAIADKALARETGARGLRSIIEDALLDVQFELPSREDV
                     SKCVVTRESIEKGQSPMLVTEPSPVADPHDEDLFGEEQTA"
     gene            3015..>3637
                     /locus_tag="PLV77_02140"
     CDS             3015..>3637
                     /locus_tag="PLV77_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC14679.1"
                     /translation="MGAPPLDRDSSDLQVTWGGHATVLIEIDGVRLVTDPVLRSRVVH
                     LRRQVPDPGTAFIEGIDAVLISHLHFDHLDIPTLKRFDRGVKVIVPGGAGPMIRKHGF
                     REVIELMEGEATEVGPVTVLAVPADHDGRRHPFGMEIDAIGFVVEGSRRILFAGDTDL
                     HPRLGELADSLEVALLPIWGWGHRLGPGHMDPRGAAQAAATLRPKNHR"
BASE COUNT          689 a         1205 c         1178 g          565 t
ORIGIN      
        1 aacaacaaga tcctcccggc tgacgtgccc gggccgctgg atcccaacat cagcaacacc
       61 tcgggcagcg gcacgatcgc cgtcgaaacc agtggctcga ccgccacggt cgccgaggcg
      121 gatttcaaca tgccgatcgt ctggatcccg gacccgaccg cgggcggcgc tccgatcccg
      181 atgaagttcg gtcccaaagg ggacctgacc ggcacctggg acgccgggac gggtcagctc
      241 tcccttacgg gcacactcca ggttgacgtg atctggggat tcgacgaggg cggcgcgaag
      301 atctgccggt tcaccgcgcc gaacacgacc tggaccaccg ccccgaacgc gatcagcccg
      361 ggagtcgcct tcaacggggt cggcggactt tccggcaacg gtgcgatcag cgccttctgg
      421 gacgaattgc cggccggcga gtcgatcaac ggtggcgact gcggcaagcc caacgccgtc
      481 gtgcgtgacc cgggtgcggt ctggctttcg agcggcatcg ccgaagtccc ggctcccccg
      541 acctgccagg aactgggcaa ggacgggctc tggcctgact gcgtcgacat cgattggccc
      601 gatcccgatc cggtggtcga gatcaccaag gtgaccgtga ccaagggaac tgtcacagcc
      661 ggccagaagg caaagatcaa ggtcaatgtc accaactccg gggatctcgc cgcgaccccg
      721 atcaccatca agctcgcttc gagctcgaag caggtcaagg tgcccggtca ggtcaagctg
      781 aacgtcccgg ccggcaagac cgcgaccgcg tcgatcaccg tgaccgcgac caaaaatgcc
      841 cggggcaagg cgacgatcac cgccagtacc tcagggaagt ccggcaaggc cgtggtgacc
      901 gtaaagaagc cgaggaagcg caagtaacgc gagcgcttca cccgacgaaa gcgcggagtg
      961 agttgccttt cttcacgagc tacggagaag gcgactcgct ccgcgtcttt tgcgttcggc
     1021 cgggctcagg cttcgatctc gtattccagc caggtggact tgtgggctcc ggtgctgtcg
     1081 tagagacgct gggcggtgtg gttgtccggg gcagtggccc aggtcaggtg tgaggcgccg
     1141 gactcgcggg cgagctcgac cccggcctcg atcagggccc ggccggtccc cccgccgcgc
     1201 gcggcctcgg tcacgaaaag gtcgttcatc agcacgacgt cggtagccga aagcgagctc
     1261 ttgtgacggt aaaagcagcc gaagccgacc gcttcctcgc cgtcgaaggc accgagcagc
     1321 cagccatcgg cggaaccgac gccgtcttcg cccacgagga agcgggagaa gaactcccgg
     1381 ttcctgcccc ggtcgatgtc cttgacctcg tagaactcct gatagagcac gaagagcggt
     1441 tcgaagagcg gaaattcccc aggagcaact ggtcggatct cgatcatggg atcagcgtac
     1501 cggcatgatc agagcccttc cggcttccgg cgccggccgg agaagatgca ggtgttcttg
     1561 ccggggaagg gagcagggta tggtcggagt agaaggtgtc caacgtcgcc tgcgaccgca
     1621 gttgagaggc cgcggcggcg cttcttctag agtgggacca agcgagcccg ctcacgaccc
     1681 cggagggtag atggcaggtc cgaccgatac caacgagcaa cttctctgca gcttctgcgg
     1741 caaatcgcag cggcaggtga agaagctgat cgccggtccg ggtgtttaca tctgcgacga
     1801 gtgcatcgac ctctgcaacg agatcatcga cgaggagtcg gtctctgctg ctgccccggc
     1861 cgggctcgac ctcgaccgcc tgccccgccc gcaggagatc aattccgttc tcgacgagta
     1921 cgtggtcggc caggaaaccg ccaagcgggc cctctcggtg gccgtctaca accactacaa
     1981 acgggtccgc atggccgagg agggcgacgg cgacgtcgag ctgcagaagt caaacatcat
     2041 gctgctcggc ccgaccggct gcggcaagac cctgctcgcc cagacgctcg cccggatcct
     2101 caacgtgcca ttcgcgatcg ccgatgccac cgccctgacc gaggccggat acgtcggcga
     2161 ggacgtcgag aacatcctgc taaagctcat tcaggcggcc gactacgacg tggccaaggc
     2221 cgagaccggg atcatctaca tcgacgagat cgacaagatc acccgcaagg ccgacaaccc
     2281 gtcgctgacc cgtgacgtat ccggcgaagg cgtgcagcag gcgctgctga agatcctcga
     2341 aggaaccacc gcctcggtac cgccgcaggg tggccgcaag catccgcatc aggaattcct
     2401 gaccatcgac acgaccaaca tcctcttcat ctgcggcggc tcgttctcgg aactggagaa
     2461 ggtgatcgag cgacgggtcg accacaaggg aatcggcttc ggcgcggcga tcaccgagca
     2521 ggcccacgat gatcccggcg agctcttcga acaggtgctg cccgaggacc tggtcgagta
     2581 cggcctgatc cccgagttca tcggccggct gccggtggtg gcggcgcttc atcagctcga
     2641 ccagaccgac ctggtccgga tcctcaccga gcccaagcat gctctggtcc gccagttcca
     2701 gcgcttcttc gagttcgatg acatcgagct ggtcttcgcc gacgattcac tcgaagcgat
     2761 cgccgacaag gctctggccc gcgagaccgg cgcccggggg ctgcgctcga tcatcgagga
     2821 cgccctgctc gacgtccagt tcgaactgcc ctcccgcgag gacgtgagca agtgcgtggt
     2881 gacccgcgaa tcgatcgaga agggccagtc gccgatgctg gtgaccgaac cgagcccggt
     2941 cgcggacccg cacgacgagg acctgttcgg cgaagagcag accgcctgac tcttcgccgc
     3001 gggatactcc ctgcgtggga gcgccgcccc ttgaccggga ttcatccgat ctccaggtga
     3061 cctggggcgg acacgccacg gtcctgatcg agatcgacgg ggtgcgcctc gtcaccgacc
     3121 cggtgctccg cagccgggtg gtccacctgc gccggcaggt gcccgacccc ggaacggcct
     3181 tcatcgaagg catcgacgcg gtcctgatct cacatcttca tttcgaccat ctcgacatcc
     3241 cgaccctgaa gcgcttcgac cgcggggtga aggtaatcgt tccgggcggc gccgggccga
     3301 tgatcagaaa gcacggcttc cgcgaagtga tcgagctcat ggaaggggaa gcgaccgagg
     3361 tcggcccggt tacggtcctc gcagtgcccg ccgaccacga cggtcgccgc catcccttcg
     3421 ggatggagat cgatgcgatc ggcttcgtgg tcgaaggttc gcggcggatc ctgttcgccg
     3481 gtgacaccga cctgcatccg cggctcggtg aactggccga cagcctggaa gtggccctgc
     3541 tgccgatctg gggctggggt cacaggttgg gtcccggcca catggacccg cgcggggcag
     3601 cgcaggcggc cgcgaccctg aggccaaaaa atcaccg
//
LOCUS       DAOQMF010000081         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13339_length_3624_cov_3.874194, whole genome shotgun sequence.
ACCESSION   DAOQMF010000081 DAOQMF010000000
VERSION     DAOQMF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13339_length_3624_cov_3.874194"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /gene="sucD"
                     /locus_tag="PLV77_02145"
     CDS             complement(<1..750)
                     /gene="sucD"
                     /locus_tag="PLV77_02145"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC14680.1"
                     /translation="MSIIIDNDTKLVVSGLTGREGSFHGLRNKAYGTRLVAGVTPGKG
                     GQDVEGTPVFNTIEESVSEAGANTAMIFVPAKFAAGSINEAIDSGVDTVIAITEGIPV
                     MDMLPTYWKAREKGVRLIGPNCPGVLSPGKANVGIIPAQFFDQGRIGVVSKSGTLTYQ
                     IGNELKQAGEGNSTIVGIGGDPIIGSDFIDILTLFDADPETDLIVMVGEIGGDAEERA
                     AEFIAAEISKPVVAYIAGFTAPPGKQMGHAGA"
     gene            complement(760..1920)
                     /gene="sucC"
                     /locus_tag="PLV77_02150"
     CDS             complement(760..1920)
                     /gene="sucC"
                     /locus_tag="PLV77_02150"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNC14681.1"
                     /translation="MDLLEYQGKELFARHGLPVPKGVHAKTADAAVDASEALGYPVAV
                     KAQVLIGGRGKAGGIKIAKDGTEAHEYAGEIIGMDIVGPHGEGPFKVHELWVEAGSEI
                     ESEFYASVILDRSAKKLMVMLSRMGGMDVEAIAASDPEALVKHHVDPLGGLGEDEAKQ
                     IAAQAKIPAEVADQVAAVLVQLVEVARAEDATLIEVNPLIVNADGKVVLLDAKVTIDG
                     NAMFRHPELEELPESADQDPQEKMAKERGLTYVSLDGNIGILGNGAGLCMSTLDVVAQ
                     AGGRPANFLDAGGGSKAEAIIDALEVITSNPEVTAILFNIFGGITRCDEVARGIIEAT
                     NKIGLTLPLVVRLDGTNSEEGLALLAEAGLPNLHHEATMLGAAKKVVELAGN"
     gene            2029..2715
                     /locus_tag="PLV77_02155"
     CDS             2029..2715
                     /locus_tag="PLV77_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC14682.1"
                     /translation="MPLPSLKNIEWVTTDCYGDLIDWEKGISEACLKEAEKDGFSFDP
                     GPFMERFFEVQAEIMAGSYELYAEVLRRTIIKVAGEIGWEVEPSRAQFLPDSVAYWMP
                     FREANAAMDRLAKKYNIGIISNIDDKMLGISRRHLRTDLDLVVTAQQVRSYKPDDTHF
                     KECARRIGGKKGWLHIGSNYDTDVAPLLKMKVPVIWVNRHGEKLAGRKKPSLEVKTFR
                     DAVKKLGAGG"
     gene            2721..>3624
                     /locus_tag="PLV77_02160"
     CDS             2721..>3624
                     /locus_tag="PLV77_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC14683.1"
                     /translation="MPALTRFRGRRYGFALAALLGAALALLLVAPLDRSLAARPNFVV
                     IQTDDQNAATVRARLRGKVGGFKPVMPHAVRQILNRGTEFRNYYASSPVCSPSRASLL
                     SGQYPWNNGVESNSGPNGGWNGWVNNPVYRQNLVTTLHRSGYRTAHFGKFTNGYFDTT
                     HGSVERKVPPGWTDWFTTSYEVGTHFYGYRLNVNGRATRPIGSLHYRQYGPGIDPRKC
                     SLRKLEDFWPLGDCNYSGDVFTAAAVREIRRENRRPFYIQIDYQAPHGDVAAPRGPQP
                     ASREIGTARRTPLPRPPSFNEADFS"
BASE COUNT          603 a         1180 c         1196 g          645 t
ORIGIN      
        1 cgctccggcg tgccccatct gcttgccggg aggggcggtg aagccggcga tgtaagccac
       61 gacgggcttg ctgatctcgg ccgcgatgaa ctccgctgcc cgctcctcgg cgtcgccgcc
      121 gatctcgccg accatcacga tcaggtcggt ctcggggtcg gcgtcgaaga gggtgaggat
      181 gtcgatgaag tcggagccga tgatcgggtc accgccgatg ccgacgatgg tcgagttgcc
      241 ctcgcccgcc tgcttcagct cgttgccgat ctggtaggtc agggtgcccg acttggagac
      301 cacgccgatc cggccctggt cgaagaactg ggccgggatg atgccgacgt tggccttgcc
      361 tggcgaaagc acgccggggc agttcggccc gatcaggcgg acgcccttct cgcgggcctt
      421 ccagtaggtc ggcagcatgt ccatcaccgg gatgccctcg gtgatcgcga tcaccgtgtc
      481 aaccccggag tcgatcgcct cgttgatcga accggcggcg aacttcgccg ggacgaagat
      541 catcgcggtg ttggcgccgg cctcgctgac cgattcctcg atcgtgttga aaacgggggt
      601 gccttcgacg tcctggccgc ccttgccggg ggtgacaccg gcgaccagcc gggtgccgta
      661 ggccttgttg cggaggccgt ggaagctgcc ctcgcggccg gtcaggccgg agacgaccag
      721 tttcgtgtcg ttgtcaatga tgatgctcat tgctgatcct cagttcccgg ccagctcgac
      781 gacctttttg gccgcgccga gcatggtggc ttcgtggtgc aggttgggga ggccggcttc
      841 ggccagcagg gccaggcctt cctcggagtt ggtgccgtcg aggcggacca ccaggggcag
      901 ggtgagcccg atcttgttgg tggcctcgat gatgccgcgg gcgacctcgt cgcagcgggt
      961 gatcccgccg aagatgttga agaggatcgc cgtgacctcg gggttcgagg tgatcacctc
     1021 gagtgcgtcg atgatcgcct cggccttcga tccgccgccg gcatcaagga agttggcggg
     1081 gcggccgccg gcctgggcga ccacgtccag cgtgctcatg cagagcccgg cgccgtttcc
     1141 gaggatgccg atgttcccgt ccagcgacac ataggtgagg ccgcgttcct tggccatctt
     1201 ctcctgcgga tcctggtcgg cggactcggg aagctcctcg agttccgggt ggcggaacat
     1261 cgcgttgccg tcgatcgtca ccttggcgtc gagcagcacg accttgccgt cggcgttgac
     1321 gatcagcggg ttgacctcga tcagggtcgc gtcctcggcc cgggcgacct cgaccagctg
     1381 gaccagcacc gcagcgacct gatccgcgac ctcggccggg atcttcgcct gggcggcgat
     1441 ctgctttgcc tcgtcttcac ccaggccgcc aagcgggtcg acatggtgtt tgaccagagc
     1501 ctccggatcg gaggcggcga tcgcctcgac gtccatgccg cccatgcggg aaagcatgac
     1561 catcagcttc ttggccgagc ggtcgaggat gaccgacgcg tagaactcgg attcgatctc
     1621 tgaaccggcc tcgacccaga gttcatggac cttgaacggg ccttcgccgt gggggccgac
     1681 gatgtccatg ccgatgatct caccggcgta ctcgtgggct tcggttccgt ccttggcgat
     1741 cttgatgccg ccggccttgc cgcggccgcc gatcagtacc tgggccttga cggcgaccgg
     1801 gtagccgagg gcttcggaag cgtcaacggc agcgtcggcg gttttcgcgt gcacgccctt
     1861 ggggacggga agcccgtggc gggcgaacag ttcttttccc tgatattcga ggagatccat
     1921 gacctttgag gagttgggag ttggagggct ccggaaggca tggccgaagg tactttgggc
     1981 cacgtcgaag ccgacgatgg actctattcg acataatgcc tttcccgcat gccgctgcca
     2041 tcactgaaga acatcgagtg ggtaaccact gactgctacg gagacctgat cgactgggag
     2101 aagggcatct ccgaggcctg tctgaaggaa gccgaaaagg acggattcag cttcgatccc
     2161 ggcccgttca tggagcggtt cttcgaggtg caggctgaga tcatggccgg ctcctacgag
     2221 ctctacgccg aggtgctgag gcgcacgatc atcaaggttg ccggggagat cggctgggaa
     2281 gtcgagcctt cacgggcgca gtttctgccc gacagtgtcg cctactggat gccgttccgc
     2341 gaggccaacg cggcgatgga ccggctggcc aagaagtaca acatcggcat catcagcaac
     2401 atcgacgaca agatgctcgg catctcgcgg cgtcacctga ggaccgacct cgacctggtc
     2461 gttaccgcgc agcaggttcg cagctacaag cccgatgaca cccacttcaa ggaatgtgcc
     2521 cgccggatcg gcggcaagaa gggctggctc cacatcggtt cgaactacga caccgacgtg
     2581 gcccccctgc tgaagatgaa ggtcccggtg atctgggtca accggcacgg cgaaaagctg
     2641 gccggacgca agaagccctc gctggaggtc aagaccttcc gcgacgccgt caagaagctg
     2701 ggcgccggag gctgaccaac ttgcccgccc tcacccgatt ccgggggagg cggtacgggt
     2761 tcgctctggc ggccctgctg ggtgctgcgc tggcgctgct gctggtcgcc ccgctcgacc
     2821 gctcgcttgc ggccaggccg aacttcgtgg tgatccagac cgatgaccag aacgcggcca
     2881 cggtccgggc caggttgcgc ggcaaggtcg gcggcttcaa gccggtcatg ccccatgcgg
     2941 tccggcagat cctcaaccgc ggcaccgagt tccgcaacta ctacgcgagc tcgccggtct
     3001 gcagcccttc ccgggcctcg ctgctgagcg gccagtaccc gtggaacaac ggggtcgagt
     3061 ccaactcggg cccgaacggc ggctggaacg gctgggtgaa caacccggtc taccgccaga
     3121 acctggtcac caccctgcat cgctccggtt accgcaccgc ccacttcggc aagttcacca
     3181 acggctactt cgacacgacc cacggcagcg tcgaaaggaa ggtcccgccg ggctggaccg
     3241 actggttcac gacctcatat gaagtcggga cgcatttcta cggctaccgg ctgaacgtca
     3301 acggtcgcgc cacccggccg atcggaagcc ttcattaccg gcaatacggc ccggggatcg
     3361 acccccggaa atgctcgctc cggaaactcg aggatttctg gccgcttggc gactgcaact
     3421 acagcgggga cgtcttcacc gccgccgcgg tgcgggagat caggcgtgag aaccgccggc
     3481 ccttctacat ccagattgat taccaggcac cccacggtga cgttgccgcg ccccgtggac
     3541 cccaaccggc cagccgcgag atcggcaccg cccggcggac cccgttgccg cgtccgccct
     3601 cattcaacga ggctgatttc agcg
//
LOCUS       DAOQMF010000082         3613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13405_length_3613_cov_4.206858, whole genome shotgun sequence.
ACCESSION   DAOQMF010000082 DAOQMF010000000
VERSION     DAOQMF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13405_length_3613_cov_4.206858"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLV77_02165"
     CDS             complement(<1..458)
                     /locus_tag="PLV77_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNC14684.1"
                     /translation="MKVALCGLGEIGQIHLRAIRESGHELVGVLELNRRLAVESVGDE
                     VPIHTEVMEMVELTEPDVVDICLPHHLHVPIAVAALEAGADVLLEKPMAIDLAGCDEI
                     IAAAEAFDHRVGISHNQIHFAPHRRLRELIDAGRLGQIRAIYERLWMGGK"
     gene            complement(455..1240)
                     /locus_tag="PLV77_02170"
     CDS             complement(455..1240)
                     /locus_tag="PLV77_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNC14685.1"
                     /translation="MTREISFIAALDYEERPAAEVLESLLEAGYDSVEWTMAHAEELL
                     APASALACQMDLVTGGEKAAEETIRGIEAAAAAGIGVVNVLTGPNLWEEGAVQRDDEA
                     AWKVALEALEWIVARGEAAGVAIGFEPCWGTLAHDAQTAQRVLDSVGVSVTFDPSHFV
                     MTGDPIPELIERWGERIVHFHLKDAFGRPGIDGEDFIFCMLGEGKVPWPETFAALDRV
                     GYEGPLSVEFEAYAYYEQVLGSDPAAAARLARGQVAALLGEGS"
     gene            complement(1237..2583)
                     /locus_tag="PLV77_02175"
     CDS             complement(1237..2583)
                     /locus_tag="PLV77_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC14686.1"
                     /translation="MNHDTPERDPNGAAQQLKNESDDHVIKCMGDVDMGLVKGEGVRV
                     WDADGNEYLDAISAEWVLNLGFRHPEIIEAMEAQFGKIDYVSPVFEDEARTAYAKKLA
                     ELAPGALSKVLYGLSGASAVEGAMHLAMRTTGGTDFVCLDAAFHGRTFATIGLTYVNP
                     GMVEGANKGLDRYLPRQMRVPNYNCYRCPLNLEPGTCDMACAEAVDWALDKGHLNGPA
                     GVIVEPYQANGGMVPAPDGYLERVHEIAKSHEVPLIVDEIQGALCRCGPMYASQRAGI
                     EPEMIILGKALGGGLPLSATITTPEYSELLPWEYGFTQAGNPMACAAGLAMVEIMVRD
                     DLPANSERIGTMMIEELEKIQEDSKLIGDVRGQGLMIGVELVRDRETREPAFEETDQL
                     MAACLEHGLMIGKTGPVFGANGNVAKFKPAVNVTEEDAMEMIDLFKRALGDVEATL"
     gene            complement(2607..>3613)
                     /locus_tag="PLV77_02180"
     CDS             complement(2607..>3613)
                     /locus_tag="PLV77_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNC14687.1"
                     /translation="FPEDHPLDAEHTGSNGTLVGNYMTKNADVILAVGTRFAEQTSSS
                     FMDGMSFSIPPTEIIHLDIDPTEIGKNYPTAVGAVCDAKAGLNDLFDALVDHNGTEGI
                     SRPAYREEIAKLRADWKEMLTGRWTGKLSLSQVLHKSREVLPREAIAIASAGHPQIQA
                     FQEFLSYEPRTWLSPGGYSTMGYTLPAAIGAKLAKPDVPVVGFAGDGDLQQTIQELAV
                     AAETGAAVIIACLNNTGWISIRDFQRGMFGEDRGFETEFRSRKGDQLMPVDFCAIAEA
                     FNCGSEKVTETGDIGPAFERAMASEGPCLIEFELSSEPADSEGVNIGHWDLPKPAYLP
                     "
BASE COUNT          565 a         1197 c         1181 g          670 t
ORIGIN      
        1 tactttccgc ccatccacag gcgctcgtag atcgccctga tctgccccag ccggcccgcg
       61 tcgatcagct cacgcagacg ccggtgaggc gcgaagtgga tctggttgtg ggagatcccg
      121 acccggtggt caaaggcttc ggccgcggcg atgatctcgt cacagccggc gagatcaatc
      181 gccatcggct tctcgagcag cacgtcggcg ccggcttcga gcgcggccac ggcgatcggc
      241 acgtgaaggt ggtgaggcag gcagatgtcg accacatccg gctcggtcag ttcgaccatc
      301 tccatgacct cggtgtggat cggcacctcg tcaccgacgc tttcgaccgc gaggcggcgg
      361 ttcagttcca gcacaccgac cagttcgtga ccgctttcgc ggatcgccct gaggtggatc
      421 tggccgatct cgccgagtcc gcagagcgcg accttcacga gccctccccg agcagggccg
      481 ccacctggcc gcgggcgagc ctggccgcgg cagccgggtc gctgcccagc acctgctcgt
      541 aataagcgta ggcctcgaac tcgaccgaga gcgggccttc gtatccgact cggtcgagcg
      601 ccgcgaaagt ctccggccag ggaaccttgc cctcaccgag catgcagaag atgaagtcct
      661 cgccgtcgat gccgggccgg ccgaaggcgt ccttgaggtg gaagtggacg atccgctcgc
      721 cccagcgttc gatcagctcc gggatcgggt cgccggtcat cacgaaatgg ctggggtcga
      781 aggtgaccga aacgccgacc gaatccagca cccgctgggc ggtctgcgcg tcgtgggcca
      841 gggtgcccca gcagggttcg aatccgatcg caactcccgc cgcctcgccc cgggcgacga
      901 tccactccag cgcttcgagc gcaaccttcc aggctgcttc atcgtcgcgc tgaaccgcgc
      961 cctcctccca cagattcggg ccggtcagca cgttgacgac cccgatcccg gcggcggccg
     1021 ccgcttcgat gccgcggatg gtttcctcgg ccgctttttc gcctccggtg accaggtcca
     1081 tctgacaggc gagggccgag gccggcgcga gcagttcctc cgcgtgggcc atggtccact
     1141 cgaccgagtc atatccggcc tcgagcagcg actcgaggac ttccgcggcg gggcgctctt
     1201 cgtagtcgag cgccgcgatg aagctgatct ccctcgtcac agagtcgcct cgacatcacc
     1261 gagggcccgc ttgaagaggt cgatcatctc catcgcgtct tcctcggtca cgttgacggc
     1321 cggcttgaac ttggcgacgt tgccgttcgc gccgaagacc gggccggtct tgccgatcat
     1381 cagtccatgc tcgaggcagg cggccatcag ctggtcggtt tcctcgaagg ccggttccct
     1441 ggtctcccgg tcgcggacca gctcgacccc gatcatcagg ccctggccgc gaacatcgcc
     1501 gatcagcttc gagtcctcct ggatcttctc cagttcctcg atcatcatcg tgccgatccg
     1561 ctcggaattg gcaggcaggt catcccggac catgatctcg accattgcca ggcccgccgc
     1621 gcaggccatc gggttgccgg cctgggtgaa gccgtattcc cagggcagca gttccgagta
     1681 ctcgggggtg gtgatcgtgg ccgagagcgg caggccgcca ccaagcgcct tgccgaggat
     1741 gatcatctcc ggctcgatcc cggcccgctg gctggcgtac atcggcccgc agcggcaaag
     1801 agcgccctgg atctcgtcga cgatcagcgg gacctcatgt gacttggcga tctcgtggac
     1861 ccgctcgagg tagccgtccg gggcgggaac catgccgccg tttgcctggt agggctcgac
     1921 gatcaccccg gccggaccgt tcaggtggcc cttgtcgagg gcccagtcga ccgcttccgc
     1981 gcaggccatg tcacaggttc cgggttccag gttgagcggg cagcggtagc agttgtaatt
     2041 cggcacccgc atctgtcgcg gcaggtagcg gtcgaggccc ttgttggccc cctcgaccat
     2101 gccggggttc acgtaggtca ggccgatcgt ggcaaaggtc cggccgtgga aggcagcgtc
     2161 gaggcagacg aaatcagtcc cacccgtcgt ccgcatcgca agatgcatcg ctccttccac
     2221 ggccgatgcg ccggagagac cgtagagcac cttcgagagt gctcctggcg caagttcagc
     2281 cagtttcttc gcataggcgg tccgggcttc atcctcgaag accggcgata catagtcgat
     2341 cttgccgaac tgggcctcca tcgcttcgat gatctcgggg tggcggaagc cgagattgag
     2401 aacccattcg gccgagatcg cgtccaggta ttcgttgccg tcggcatccc agacccggac
     2461 gccttcgccc ttgaccaggc ccatgtccac atcgcccatg cacttgatga catggtcatc
     2521 ggactcgttc ttcagctgtt gcgcggcccc gttcgggtct ctttcggggg tgtcgtgatt
     2581 caccttgttg cctccttggg tgggggtcag gggaggtagg ccggcttcgg cagatcccag
     2641 tggccgatgt tcacgccttc actgtcggcc ggttcggatg agagttcgaa ctcgatcagg
     2701 cagggccctt ccgaggccat cgcccgctcg aaggcgggac cgatatcgcc ggtctcggtc
     2761 accttctccg agccgcagtt gaaggcttcg gcaatcgcgc agaagtcgac cggcatcagc
     2821 tggtcgccct tgcgcgagcg gaactcggtc tcgaagccgc ggtcctcgcc gaacatgccg
     2881 cgctggaagt cccggatcga gatccagccg gtgttgttga ggcaggcgat gatcacggcc
     2941 gcgccggtct cggcggcaac tgccagctcc tggatcgtct gctggaggtc accgtcaccg
     3001 gcgaagccga ccaccggcac atccggtttg gccagcttgg ctccgatcgc ggccggcagg
     3061 gtgtagccca tggtcgagta accgcccggg ctgagccagg tccgcggttc ataggagagg
     3121 aactcctgga aggcctggat ctgggggtgg ccggccgagg cgatcgcgat cgcttcccgc
     3181 ggcaatacct cccgtgactt gtgcaggacc tggctgagcg agagcttgcc ggtccagcgg
     3241 ccggtcagca tttccttcca gtcggcccgc agcttcgcga tctcttcccg gtaggcgggc
     3301 cggctgattc cttcggttcc gttgtgatcg accagcgcgt cgaagagatc gttgagaccg
     3361 gcctttgcgt cacagaccgc gccgaccgcg gtcgggtagt tcttgccgat ctcggtcggg
     3421 tcgatgtcga ggtggatgat ctcggtcggc ggaatcgaga aggacatccc gtccatgaag
     3481 gaggaggagg tctgctcggc aaagcgggtg ccgaccgcga ggatcacgtc cgcgttcttg
     3541 gtcatgtagt tgccgaccag ggtgccgttg gagccggtgt gttcggcgtc gagcggatgg
     3601 tcctcgggga aga
//
LOCUS       DAOQMF010000083         3602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13459_length_3602_cov_3.765436, whole genome shotgun sequence.
ACCESSION   DAOQMF010000083 DAOQMF010000000
VERSION     DAOQMF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13459_length_3602_cov_3.765436"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLV77_02185"
     CDS             complement(<1..404)
                     /locus_tag="PLV77_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNC14688.1"
                     /translation="MSQEQMNQKLKDKRVAILVANEGVEEIELKGPLQALRDAGAEVD
                     LVAPEEGQVQAFNHLDKGSPFTVDRTVSEVSSDEYAALVLPGGVANPDNLRTHEDAVA
                     FAGSFFEAGKPVGAICHAPWTLVEAGVLEGRTL"
     gene            536..1129
                     /locus_tag="PLV77_02190"
     CDS             536..1129
                     /locus_tag="PLV77_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNC14689.1"
                     /translation="MKALTINCTLKASPATSNTEALATVVADEIQQAGVETDLVRAVD
                     HNILPGVSNDEGEGDGWPAVLDRVRAAQILIIATPTWLGQPSSVAKRVLERMDALISE
                     TDDEGVPFAYGRVAGVVVTGNEDGAHHVISEISGSLIDIGYTIPGQAWTYWNRGPGPG
                     DDYLSNEAGHDWSHETGKTAARNLLTAARSLEGAWSG"
     gene            complement(1134..2051)
                     /locus_tag="PLV77_02195"
     CDS             complement(1134..2051)
                     /locus_tag="PLV77_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNC14690.1"
                     /translation="MPRPLWKGTISFGLVSVPVEMWSAIRDRNIRFREIHVEDGSPVR
                     HVRYCAEEGKPVDPSEVGRAYELDDGRLVRITDEELEGLEPEKTQTIEIESFAPRAQV
                     EPLRFDRPYNLIPGNQTVGTIRAYRLLVEAMAESDLLALGKFVMRGREYLAAIRVREN
                     VLTLSTMHFPAEIRSTEDVPGPAKKADKEAVKDAVAVIEERSVEWDPGKYPNEHRERL
                     MAVIRKKDKGRKIKAPAPVAEERPTGAPDLMAALKKTLAESRSKRRKPRDLARLSKEE
                     LYEMASERGLKGRSSMNKQQLLKALRANR"
     gene            complement(2051..2830)
                     /locus_tag="PLV77_02200"
     CDS             complement(2051..2830)
                     /locus_tag="PLV77_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNC14691.1"
                     /translation="MALRSIWNGTISFGLVKVPIKLYSATEPKGIAFREIHVKDNSLL
                     QHRRVCKAEGREVDRSEIAKGYEVRPDQYVVLESDEVKAAAGERPKTIEIEEFVDVEA
                     IDPFYFNKSYFMGVRDVDEPYALLVAALRDSGKAGIGRFTFHNREYLVALHAEEDRLV
                     LHTLRYAEEIIEPGDLELPDGGKKPTRKEVELARRLVEGLTEDFDPGEYQDEYRASVM
                     DLIERKAEGKKPKKKRARKREETGDLSKALEKSLAAQGSRS"
     gene            2923..>3602
                     /locus_tag="PLV77_02205"
     CDS             2923..>3602
                     /locus_tag="PLV77_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HNC14692.1"
                     /translation="MKARLGDPGKGVAWNFEVKWDGYRAIAFCSGGLKLQGRRLNEIG
                     ADFPEIKPLGSDSRARGLILDGELVVVDEDGRPDFQLMQSRRERQLEAHFMIFDLLWA
                     DGTDLRPMPYLERRARLEALGLTGPTWSVPERLAGELNEVLEATAALGLEGVVAKDPD
                     SPYVSGRRTNYWLKVKHVKRQEFVIGGWLPGKGHRSGSLGSLLVGYYDGNSELRLAGR
                     VGTGMGDEL"
BASE COUNT          590 a         1204 c         1108 g          700 t
ORIGIN      
        1 agcgttcggc cttccaggac gccggcctcg accagggtcc agggggcatg gcagatcgct
       61 ccgaccggct tgcccgcctc gaagaaggaa ccggcaaagg cgaccgcgtc ttcgtgggtt
      121 ctcaggttgt ccgggtttgc cacgccaccc gggagcacga gggctgcgta ctcgtcactc
      181 gaaacctcgg aaacggtccg gtccacggtg aagggacttc ccttgtcgag gtgattgaag
      241 gcctggacct gaccctcctc gggtgcgacc aggtcaactt ccgctccggc gtccctcagc
      301 gcctgcagcg gccccttcag ttcgatttcc tcgacgccct cgttggccac gaggatcgcc
      361 acacgcttgt ccttcagctt ctgattcatc tgttcctggc tcatcgtttc tcctcttgtg
      421 ggttcaatta ccggttaccc gttcggcccc cggtcaaccc tggatccgcg tccggacccg
      481 ccctgattct ggtggcggca cgttttggcc ttcccggcag gggtaccggc cgggaatgaa
      541 agcgttgacc atcaactgca ccctgaaggc cagtccggcc acctcaaaca ccgaagccct
      601 ggcaaccgtg gtcgccgacg aaatccagca ggccggggtc gagaccgacc tggtccgggc
      661 ggtggaccac aacatccttc ccggagtcag caacgatgaa ggcgaaggtg acggctggcc
      721 tgcagtgctc gaccgggtcc gcgcggcgca gatcctgatc atcgcgacgc cgacctggct
      781 cggtcagcct tcgagcgtcg ccaagcgggt gctggaacga atggacgcgc tgatcagcga
      841 gaccgacgat gaaggcgttc cgtttgccta cggccgggtc gccggcgtgg tggtgaccgg
      901 gaatgaagac ggcgcgcatc atgtgatcag cgagatctcc ggcagcctga tcgacatcgg
      961 ctacacgatt cccggtcagg catggaccta ctggaaccgg ggtcccggac cgggtgatga
     1021 ctacctttcg aatgaagccg ggcacgactg gtcccacgag accgggaaga ctgcagccag
     1081 gaacctgctc accgccgccc gttccctcga aggagcctgg tcgggctgac cttctagcgg
     1141 ttggcgcgga gcgccttgag cagctgctgc ttgttcatcg aggagcggcc cttgaggcct
     1201 cgttcggagg ccatctcgta aagctcctcc tttgagagcc gggcgaggtc gcgcggcttc
     1261 ctgcgcttgc tgcgggattc cgccagggtc ttcttcaggg cggccatcag atccggggca
     1321 ccggtcggcc gctcttcggc gacgggagcg ggagccttga tcttccggcc cttgtccttc
     1381 ttcctgatca ctgccatcag ccgctcacga tgttcgttcg gatacttgcc gggatcccat
     1441 tcgacggagc gttcctcgat caccgcgacg gcgtccttga ccgcctcctt gtccgctttc
     1501 ttcgccggtc ccggcacgtc ctccgtagat cgaatctcgg ccgggaaatg catcgtcgag
     1561 agcgtgagaa cattttcccg gacccggatc gcggccaggt actcccgtcc ccgcatcacg
     1621 aacttgccca acgcaagcag gtcgctttct gccatcgcct cgaccaggag acggtacgca
     1681 cggatcgtgc ccacggtctg gttgccggga atcaggttgt acggccggtc aaagcggagc
     1741 ggttcgacct gggcgcgcgg ggcgaaggat tcgatctcga tggtctgtgt cttctccggt
     1801 tcgagtccct ccagttcctc gtcggtgatc cggaccaggc ggccgtcatc gagttcgtag
     1861 gcacgaccga cttccgaggg atcgaccggt ttgccttctt cggcgcagta gcgcacgtgc
     1921 ctgaccggcg aaccgtcttc cacgtggatt tcacggaacc tgatgttgcg gtcccggatc
     1981 gccgaccaca tctcgaccgg cacgctgacc aggccgaacg aaatcgtccc cttccagagt
     2041 ggtcgcggca tcaggaacga ctcccctgcg cggcgaggct cttttcgagc gccttggaaa
     2101 ggtccccggt ctcctcacgc ttgcgggccc gcttcttctt cggcttcttg ccctcggcct
     2161 tccgctcgat cagatccatc acggacgcgc ggtactcgtc ctggtactcg ccggggtcga
     2221 agtcctcggt cagtccctcg accagccggc gcgccagctc gacttccttg cgggtcggct
     2281 tcttgccgcc gtccggcagt tcgaggtcac ccggctcgat gatctcctcg gcgtacctca
     2341 gtgtgtggag caccagcctg tcctcctcgg cgtgaagggc gaccaggtac tcgcggttgt
     2401 ggaaggtaaa ccggccgatc cctgccttgc cgctgtcccg gagtgccgcg acaagcagcg
     2461 cgtaaggctc gtccacatcc cgcacaccca tgaagtagga cttgttgaag tagaagggat
     2521 cgatcgcctc gacatcgacg aactcctcga tctcgatcgt cttcggccgc tcgccggccg
     2581 ccgcctttac ctcatcactc tccagcacga cgtactggtc ggggcgcacc tcatagccct
     2641 tggcgatctc gcttcggtca acttccctgc cctccgcctt gcagacccgg cggtgctgca
     2701 gcagcgaatt gtccttcacg tgaatctcgc gaaaggcgat tcccttcggt tcggtcgcag
     2761 agtacagttt gatcgggacc ttgaccagcc cgaaggagat ggttccgttc cagattgacc
     2821 gcaacgccat gcggtgagcg tacccgcgaa ccgcttcatc cacaccatcg cgggtattcg
     2881 gtagacatga tgcaagcgat gttgcccggt ccaatcgaac ccatgaaagc ccggctcggc
     2941 gatcccggca agggggtcgc ctggaacttt gaagtcaagt gggacggtta ccgggccatc
     3001 gccttctgtt ccggaggcct gaaactccag ggccggaggc tcaacgagat cggcgccgac
     3061 tttcccgaaa tcaagccgct cggttccgac tcccgcgccc gcggcctgat cctcgacggc
     3121 gagctggtgg tggttgacga ggatggccgg ccagacttcc agttgatgca gtcccgccgc
     3181 gagcgtcaac tcgaagcgca cttcatgatc ttcgacctgc tctgggccga cggcaccgac
     3241 ctcaggccga tgccctacct cgaacgccgc gcgcggctgg aggctctcgg tctaacgggc
     3301 ccgacctggt cggtcccgga acggctcgcc ggagagctga acgaggtgct cgaagccacg
     3361 gccgcactcg ggctcgaagg ggttgtcgcc aaggatcccg acagccccta tgtgtcgggc
     3421 cgacggacga actactggct caaggtcaag cacgtcaaac ggcaggaatt cgtgattggc
     3481 ggctggctgc ccggcaaggg tcaccgttcc ggttcgctgg gttcgctcct ggtcggctac
     3541 tacgacggca actccgaact ccggctggcc ggcagggtcg gcaccggaat gggtgacgaa
     3601 ct
//
LOCUS       DAOQMF010000084         3587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13571_length_3587_cov_3.358437, whole genome shotgun sequence.
ACCESSION   DAOQMF010000084 DAOQMF010000000
VERSION     DAOQMF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13571_length_3587_cov_3.358437"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLV77_02210"
     CDS             <1..538
                     /locus_tag="PLV77_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14693.1"
                     /translation="RIAPLAAMAAGPGRAIRNERGKLNQKTREIRAAADRGIQGRRGS
                     AQRVLDQCLLPAASRTNRLIEAAGTRNGARPGALQARVLGRLGFAGRKLETERVALRA
                     HDPQRTLERGYARVEDRKGDPVVDSSGARRAGQVRIRFADDAVDAVVGDTRRRRSRQN
                     EVEQEFEQIRMEGLDEGE"
     gene            531..863
                     /gene="xseB"
                     /locus_tag="PLV77_02215"
     CDS             531..863
                     /gene="xseB"
                     /locus_tag="PLV77_02215"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNC14694.1"
                     /translation="MSEETPEAPEHSYESATARLDEIIQRLDSGDAQLRETLELCEEA
                     KGLIEYCAGELAAVDQGLKELKLEDLASSLTGPGNGGSEPPVPEPAGPPEPEHEVPPP
                     PDQPDVPF"
     gene            complement(842..>3587)
                     /locus_tag="PLV77_02220"
     CDS             complement(842..>3587)
                     /locus_tag="PLV77_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC14695.1"
                     /translation="NYGTAYDRAKDRRGGLDFEDLQLKTLDLLLRREAIADSYREQFA
                     EIMVDEFQDTNRLQMDLIDKLRGEETTLFTVGDEMQAIYGFRYADVELYRQRRAGISF
                     RLPADAERRAAGERDRVTFEVTTLPLSANFRSQAPVIGAVNELGNQFESLIEGIRTDD
                     EPGGETGGTRHEFEHLRVGWSPPEPGSGPAPPPEPKVEILLTKAKAWQQVDLGPLSPA
                     PTGDQYMTESRGQHEAEALALARHIRKAVDDGIPPGDIVVLLRTKTRMWLFEAALKQA
                     GLRPYVVGGTGFWETREGIDMRALLATVANPLDDEALAGALAGPACGLSPSALWLLGS
                     RRDHDQPLWPLVSNLADKPDPEISEEDNRRAGEFVATIRRLRAKLAGLPLSAVVQTAV
                     SETGYDLVNLMRDPSGSGLANIRRVAEIASEFEATEGRDLRGLIEWIDDSARLDSEQA
                     VATEDEESDVVRLMTIHKSKGLEFPMVCVADLGLASKADSENVIWISPRNDDPGKFEV
                     GLRLPEPGGPGLDLFAWQTLKDQARTDKTDEELRILHVAMTRAESRLVLSGVTDFEKI
                     PDPTEANPFATRLTQAYGVEDFPGTIPVPAPDFLVAPAETPAISIEVILNRPDDAGDL
                     AEVTSPESLNRADASTGEPPIGRPVSPGFPEVPLSYSALASYRECPARFFATRVLKLD
                     EPERRHGDAKQPLDPEMEIRKEIDATSFGVAVHELLELLPARRWIRPADAEIDSRLEA
                     NGVPGADAGDLAREMLDGFLASELAGEIKGRRAEVETGLLVEVGGVMVRGFADLLVRD
                     PVKPLILDYKTNQLEETSIQELMSKYDLQRDLYALAVSKAAGVEELETAFVFLRAPDQ
                     PVRDHYDSKRLSEAENSIAGLIDDIVAGRYLGGPDAGRQPCGDCWACETLGVQKGTSG
                     "
BASE COUNT          593 a         1200 c         1179 g          615 t
ORIGIN      
        1 ccggattgcc ccgctcgcgg cgatggcggc cggtccgggg cgggcgatcc gcaacgagcg
       61 cggaaagctg aaccagaaga cgagggaaat ccgggccgcc gccgaccgcg ggattcaagg
      121 ccgtcgaggc tcggcccagc gggtgcttga tcagtgcctg ctcccggcgg cttcccggac
      181 caaccgcctg atcgaggccg ctggtacccg gaacggggcc cggcccggcg cgctccaggc
      241 cagggtcctg ggccggctcg gattcgccgg gaggaagctc gagaccgagc gggtcgcgct
      301 ccgggcccac gatccacaaa ggaccctcga gcggggttat gcccgggtcg aggaccgcaa
      361 gggagatccg gtagtcgatt ccagcggagc ccgtcgggcc ggccaggtca ggattcgttt
      421 cgccgacgac gcggtcgatg ccgtggtcgg cgacacgaga cgccgccggt cgcgccagaa
      481 tgaagtcgag caggagttcg agcagatcag gatggaagga ctggacgaag gtgagtgaag
      541 aaacgcccga agcgcccgag cacagctacg agtcggccac cgcgcggctc gacgagatca
      601 tccagcgcct cgactcgggc gacgcccagc tccgcgaaac cctcgaactc tgtgaggaag
      661 ccaaaggcct gatcgagtac tgcgccggag aactcgccgc cgtcgaccag ggcctcaagg
      721 aactcaagct agaggacctc gccagctccc tgaccggccc cggaaatggc ggctcggagc
      781 caccggtccc cgaaccggcc gggccgcccg agccggagca cgaggttccg ccacccccgg
      841 atcagcccga cgtccccttc tgaactccga gggtttcgca ggcccagcaa tcgccgcagg
      901 gttgtcttcc cgcgtccggt ccgcccaggt accggccggc cacgatgtcg tcgatgagac
      961 cggcgatcga attctcggct tccgagagac gctttgagtc gtagtggtcg cgcaccggct
     1021 gatccggcgc tcgcagaaag acgaacgcgg tttccagttc ttcgacaccg gcggccttcg
     1081 acactgccaa cgcgtaaagg tcccgctgga ggtcgtactt cgacatcaac tcctggatcg
     1141 aggtctcctc gagctggttt gtcttgtagt cgaggatcag gggtttgacc gggtcgcgga
     1201 cgaggagatc ggcaaagccg cggaccatca cgcccccgac ctcgaccagc agaccggttt
     1261 caacctcggc ccttctgccc ttgatttccc cggcgagttc ggaagcaaga aacccgtcga
     1321 gcatctcccg ggccaggtcg ccggcatctg ctcccggaac cccgttcgcc tccagtctcg
     1381 agtcgatttc ggcatcggcc ggcctgatcc agcggcgtgc gggcagcagt tccagcagct
     1441 cgtgcaccgc caccccgaat gaggtggcat cgatctcctt gcggatctcc atttccggat
     1501 ccagcggttg cttcgcgtcg ccgtgccggc gttccggttc gtccagcttc agcaccctgg
     1561 tggcgaagaa ccgggcggga cactcccggt aggaggcaag ggcggagtag gagagcggga
     1621 cttccggaaa accgggagat accggccggc caattggcgg ctcgccggta gacgcatctg
     1681 cccggttcag gctttccgga ctggtcactt cggccagatc cccggcatcg tccggccggt
     1741 tcagaatcac ctcgatcgag atcgcggggg tttcagccgg tgcgaccagg aaatccgggg
     1801 ccggcacagg gatggtgccc gggaagtctt cgaccccgta ggcctgggtc aggcgggtcg
     1861 caaaagggtt ggcttcggtc gggtcaggga tcttctcgaa gtcggtgacg ccgctcaaca
     1921 cgagccggct ctcggctctg gtcatggcga cgtggaggat ccgcagttct tcgtcggtct
     1981 tgtctgtcct ggcttgatcc ttaagcgttt gccaggcgaa cagatcaagc ccgggtcctc
     2041 ccggttcggg cagtcgcagt ccgacctcga acttgcccgg atcgtcgttc cgtggcgaga
     2101 tccagatcac gttttcggaa tcggccttcg aggccagacc gaggtcggcg acgcaaacca
     2161 tcgggaactc aagacccttc gacttgtgga tcgtcatcag gcgtaccacg tccgactctt
     2221 cgtcttcggt tgccaccgcc tgctccgagt ccagtcgcgc gctgtcgtcg atccactcga
     2281 tcagcccccg cagatccctg ccctctgtcg cttcgaactc gctggcgatc tcggccaccc
     2341 gccggatgtt ggccagtccg gaaccagacg gatcccgcat cagattgacg aggtcgtagc
     2401 cggtttccga aaccgcggtc tggaccacgg ctgaaagcgg caagcccgcc agcttcgcgc
     2461 gaaggcgccg gatggtcgcc acgaactcgc cggcccgccg gttgtcttcc tcggagatct
     2521 ccggatccgg cttgtccgcc aggttcgaga ccagcggcca gagcggctga tcgtgatcgc
     2581 gacggctgcc gagcaaccag agtgcactcg ggctgagccc gcaggccgga ccggccaaag
     2641 cgccggccag cgcctcgtca tcgagcgggt tcgcgacggt cgccagcaac gccctcatgt
     2701 caatgccttc gcgggtctcc cagaaacccg tgcccccgac cacgtagggc ctcagtccgg
     2761 cctgcttgag cgcggcttcg aagagccaca ttctcgtctt ggtcctgagc agaacgacga
     2821 tgtcgccggg cggtatcccg tcgtctacgg ccttgcgaat gtgacgggcc agggccagcg
     2881 cttcggcttc gtgctgcccg cggctctcgg tcatgtactg gtctccggtc ggcgccggtg
     2941 agagcggtcc gagatcaacc tgctgccagg ccttggcttt ggtcagaagg atctcgacct
     3001 tcggttcggg gggcggggcc ggcccggagc ccggctccgg cggtgaccag ccgaccctga
     3061 gatgctcgaa ctcgtggcgg gtgccaccgg tctctccgcc cggttcatcg tcggtcctga
     3121 tgccctcgat tagtgattcg aactggttgc cgagctcgtt gaccgcaccg atcaccgggg
     3181 cctgggagcg gaagtttgcg ctcagcggga gagtcgtgac ctcgaaagtg acccggtccc
     3241 gctcaccggc cgcgcggcgt tcggcatcgg cgggaagccg gaacgagatg ccggcacgcc
     3301 gctgccggta gagctcgacg tcggcatagc ggaatccgta aatcgcctgc atctcgtcgc
     3361 cgaccgtgaa cagggtcgtc tcttcgccac ggagcttgtc gatcagatcc atctgcaggc
     3421 ggttcgtgtc ctggaactcg tcgaccatga tctcggcgaa ctgctcgcgg taggaatccg
     3481 cgatcgcctc gcgtctcagg agcagatcga gagtcttgag ctgtaggtcc tcgaaatcga
     3541 gccccccgcg ccggtccttg gcccggtcgt aggcggtgcc gtagttg
//
LOCUS       DAOQMF010000085         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13628_length_3578_cov_4.180244, whole genome shotgun sequence.
ACCESSION   DAOQMF010000085 DAOQMF010000000
VERSION     DAOQMF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13628_length_3578_cov_4.180244"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLV77_02225"
     CDS             <1..651
                     /locus_tag="PLV77_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14696.1"
                     /translation="QLKKSAVNSAKVRDRSLLAKDFRKGQIPHGATGAQGAPGMIGPT
                     GAAGATGASGIPGAVGATGPTGAAGVTGENGDPGQPGGAGPTGPTGVTGPSTTAVSSG
                     VVVNLGLGPDYFPISGASSPQGAPDTVASLSPGVPVTASNLDVKLTAAPFAGFPRIVS
                     LLDDGIAVLSCVIASGTTCHADTSALIDPGSELVMVSSVLAFPSITTARFGFTLGP"
     gene            702..1502
                     /locus_tag="PLV77_02230"
     CDS             702..1502
                     /locus_tag="PLV77_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNC14697.1"
                     /translation="MGNRGKGLKGFKSKLRRPNFAETIAVIALFVALGGASYAAIKIP
                     KNSVGTKQLKKNAVTSAKVKNRSLLAADFKAGQLPAGATGATGLVGPRGATGEQGIPG
                     FPGTPGTPGINGIDGLPGSPGPAGATGATGATGSTGATGTGGFSAMTGRTSLGSSTQF
                     FAVSGSSVAGATATGLGMITPAVPVTLKNLMVVTDTAPGTGITRTFVIQEDGINVISC
                     TLGGIATACFNTSGGGVINAGSTLTMFDTVTGGSAAPSEARWGFTIGS"
     gene            1529..2494
                     /locus_tag="PLV77_02235"
     CDS             1529..2494
                     /locus_tag="PLV77_02235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNC14698.1"
                     /translation="MSERGKVVILRELLPAGRQLLEEKFQVTSLGLDADRSQMKAAAA
                     GADGLVTDLTFAVDDELLGAAGPQLKVVANFGVGYDNVDLAACAARGVTVTNTPGALT
                     NATAELALALAMAAARDLPAAEKDLREGAWQGWDPRRWRGFELSGASIGVVGAGRIGG
                     RFMELVAGFGGERLYTSRQPKPDLENRLGAERVELDELLARSDLISLHLPGSRESRHL
                     IDERALRLMKRTAVLVNTGRGTLVDSAALIRALKEDRIGAAGLDVYEGEPDFDPGFLT
                     APRVALAPHIGSATYRSRDEMSRLVAANVIAVLEGREPPNPVPGV"
     gene            complement(2543..3181)
                     /locus_tag="PLV77_02240"
     CDS             complement(2543..3181)
                     /locus_tag="PLV77_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNC14699.1"
                     /translation="MGSSERTERLAASPDLFENRLLNFFSRVHPVVPLLIYGPIIALL
                     VWLGVDRGLSALATVGLFVGGILIWTLSEYWLHRLLFHWTPKFRGGDKINFIIHGVHH
                     DHPNDKMRLVMPPAASIPLALIFYAAFMLIFGSPYGFVLFAGFLTGYLGYDYTHYYLH
                     HFVPKSSIGKKVREHHMRHHFQDHHYGYGVSTPFWDHVFRTVPRSRKADRGS"
     gene            3239..>3578
                     /locus_tag="PLV77_02245"
     CDS             3239..>3578
                     /locus_tag="PLV77_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNC14700.1"
                     /translation="MSETATATQDFKVADLSLADYGRKEIRLAEHEMPGLMATRREFA
                     DSKPLAGARITGSLHMTIQTAVLIETLVELGADVRWASCNIFSTQDHAAAAIAVGPNG
                     TPEDPQGVPVY"
BASE COUNT          641 a         1143 c         1221 g          573 t
ORIGIN      
        1 caactgaaga agagcgcggt caactcggcc aaggtccggg accgctcgct gctcgccaag
       61 gatttcagga agggccagat tccccacggg gccaccggcg cccagggtgc tccgggcatg
      121 atcggcccga ccggcgccgc aggggccacc ggcgccagcg gaatccccgg tgcggtcggt
      181 gctaccggcc cgaccggtgc ggccggggtc actggcgaga acggcgatcc ggggcaaccc
      241 gggggtgccg gaccaaccgg tccgaccggg gtgaccgggc ccagcacgac cgcggtcagt
      301 tccggggtcg tggtcaatct cgggctcggc cccgactact tcccgatcag cggggcttcc
      361 tcgcctcagg gagcgcccga caccgtggcc tcactctcgc ccggggttcc cgtcaccgct
      421 tccaacctcg acgtgaaact gaccgccgcc cccttcgcgg gatttcctcg catcgtcagc
      481 ctcctggatg atggtatcgc ggtgctcagt tgcgtcatag cctcgggtac cacttgccac
      541 gcggatactt cagcgctgat cgatcccgga agcgagctgg tgatggtcag ctcggtcctg
      601 gcgttcccga gcatcaccac cgcgcgcttc ggcttcacgc tcgggccatg atccgatcga
      661 aggctctggc aatttccggg ctctgcgggt agggtcggca catggggaac cgggggaagg
      721 gcctgaaggg attcaagtcg aagcttcgtc ggccgaactt cgccgaaacg atcgcggtca
      781 ttgcgttgtt cgttgcgctg ggcggcgcct cgtatgcggc gatcaagatc ccgaagaaca
      841 gcgtcggcac caaacagctg aagaagaacg cggtgaccag cgccaaggtc aagaaccgtt
      901 cgctgctcgc ggccgatttc aaagccggcc agctgccggc cggagcgacc ggtgcgaccg
      961 gactggtcgg ccccaggggg gcgaccggag aacagggaat tccgggattc cccgggacgc
     1021 cggggacgcc cgggataaac ggcatcgacg gtttgcccgg ttcgccaggc cccgcgggag
     1081 cgacgggagc gaccggagcc accggttcca ccggtgcgac cggtactggc ggattctcgg
     1141 cgatgaccgg acgtacctcg cttggcagct cgactcagtt cttcgcggtt tcggggtcca
     1201 gtgtcgccgg cgccaccgca accggcctgg ggatgattac gccggcagtt ccggtcacgc
     1261 tcaagaacct gatggttgtc accgacaccg cgcccggcac cggaatcacc cggactttcg
     1321 tgattcagga agacggaatc aacgtgatca gttgcaccct gggcggcatt gccactgcct
     1381 gcttcaacac ttccggtggc ggcgtcatca acgcgggaag caccctgacg atgtttgaca
     1441 cggtgaccgg cggctcggcg gctccttccg aagcccgctg gggattcacg atcggttcct
     1501 gaggcgccgg ccgtgtagcg tctggcctgt gagcgaacgg gggaaagtcg tgattctgcg
     1561 cgagctgctg ccggccgggc gccagttgct cgaggagaaa tttcaggtga cttcgctggg
     1621 tctcgacgcc gaccggtcac agatgaaagc cgcggctgcc ggagccgatg gccttgtcac
     1681 cgacctgacc ttcgcggtcg atgacgagct gctgggtgcc gccggcccgc aactcaaagt
     1741 ggtggcgaat ttcggggtgg gctacgacaa cgtcgacctt gccgcctgtg ccgcgcgcgg
     1801 agtcacggtg accaacacgc ccggtgccct gaccaacgcc acggcggagc ttgcactcgc
     1861 gctggcgatg gctgcggccc gcgacctgcc ggcagccgag aaggacctgc gggaaggggc
     1921 ctggcagggg tgggacccga ggcgatggcg cggcttcgaa ctcagcgggg cgagcatcgg
     1981 agtggtcggt gccggccgga tcggtggccg cttcatggaa ctcgttgccg gtttcggcgg
     2041 ggagcggctc tacacctcgc gccagcccaa gcccgacctt gaaaaccggc tgggagccga
     2101 aagagttgaa ctcgacgagc tgctcgcccg ctcggacctg atcagccttc acctgccggg
     2161 cagccgggaa tcccgccacc tgatcgacga acgcgcgctg aggttgatga aacggaccgc
     2221 ggtgctggtc aacaccggtc gcggcaccct ggttgattcc gcggccctga tcagggcgct
     2281 gaaggaagac cggatcggcg cggcggggct cgatgtgtac gagggcgagc ctgatttcga
     2341 tcccgggttc ctgaccgcgc cccgggttgc gctggctccc cacatcggct cggcaaccta
     2401 ccgctcgcgg gacgaaatgt cccgactggt tgccgccaac gtgatcgccg tcctcgaggg
     2461 ccgggaaccg ccaaacccgg taccgggcgt ttgatcattt cggcctgctg aagaccagcg
     2521 accgttcgcc cgggaaggcc ggtcaggatc cgcgatcggc cttgcgcgaa cgcggaacgg
     2581 tgcggaagac gtggtcccag aacggggtcg agacgccgta cccgtagtga tggtcctgga
     2641 agtgatggcg catgtgatgt tcacgcacct tcttgccgat cgatgacttc ggtacgaagt
     2701 gatgaaggta gtagtgcgtg tagtcgtagc cgaggtagcc ggtcaggaaa ccggcgaaaa
     2761 ggacgaaacc gtacggggag ccgaagatca gcatgaacgc cgcgtaaaag atcagggcca
     2821 gcgggatgct ggcggccggg ggcatgacca gccgcatctt gtcgttcgga tggtcgtggt
     2881 gaaccccgtg aatgatgaag ttgatcttgt cgccgcccct gaacttcggc gtccagtgga
     2941 aaagcaggcg gtggagccag tactcggaaa gggtccagat caggattccg ccgacgaaaa
     3001 ggccgaccgt ggcgagggcc gagaggccgc gatcgacgcc gagccagacc agcagcgcga
     3061 tgatcggccc gtaaatcagc aggggcacga ccgggtggac ccgggagaag aaattgagca
     3121 gccggttctc gaagaggtca ggggaagcgg cgagcctctc ggtgcgttcg gacgatccca
     3181 ttgcctgtgg aatggtagcg tgattggtca ccctaactct caggagaaac ccgcagtgat
     3241 gagcgaaacc gccaccgcaa cacaggactt caaggtcgcc gatctttccc tcgccgatta
     3301 cggacgcaag gaaatccgcc tcgccgagca tgaaatgccc ggtctgatgg cgacccgccg
     3361 cgagtttgcc gactcgaagc cgctggcggg agcccggatc accggctcgc tccacatgac
     3421 catccagacc gccgtcctga tcgaaaccct ggtcgaactc ggcgccgatg tgcgctgggc
     3481 ttcctgcaac atcttctcga cccaggatca cgcggccgcg gcgatcgcgg tcggcccgaa
     3541 cggcaccccc gaggacccgc aaggcgtccc ggtctacg
//
LOCUS       DAOQMF010000086         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13629_length_3578_cov_4.046551, whole genome shotgun sequence.
ACCESSION   DAOQMF010000086 DAOQMF010000000
VERSION     DAOQMF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13629_length_3578_cov_4.046551"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLV77_02250"
     CDS             <1..996
                     /locus_tag="PLV77_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC14701.1"
                     /translation="GLCHSDLSVITGQRPRVMPMALGHEAAGEVVATGPGDHGFVEGD
                     HVVLAFVPACGECRFCAQERPALCVPGGEANVEGTLLGGHIRLFEESGTEVHHHLGVS
                     ALSDHAVVSARSAVKVPDDLPFDIAALFGCAVLTGAGAVLNTGAAALPARSVAVFGLG
                     GVGLAALLAAKSQNPETLVAVDTVPAKLELARELGADLTIQAGPDLVDSIREATGGGV
                     DLAIETVGNENVLADAYAATGRGGTTVTAGLPHPSRKLEIPAISLVAEERTLKGSYLG
                     SCVPRRDIPAFIRMYRDGELPVEKLLSHRLTPDQINEGFDRLARGEAVRQIVIFD"
     gene            complement(1060..2445)
                     /locus_tag="PLV77_02255"
     CDS             complement(1060..2445)
                     /locus_tag="PLV77_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC14702.1"
                     /translation="MNVVRRGSLAFLATVAALGIFMSGSASAKLNVMTVQGTPAPGTP
                     AKYNKFKVVKQGPAKAKKILLLEPGTSAGATNFRPLGNALVGQLKGWQVWSIERRENL
                     LEDHSMLEKYLKGDATNEEMLDYYLGWILDGSVQPHFEGKTTAETQYAKKWGLSIVVH
                     DLRKVVNMARKGGRKVVLGGHSLGGSIVTAYATWNFNGTPGAKGLSGLVFIDGAGARE
                     AADLPTAGEAQANLDSLNAESASPFITLVPPLPWAAGVFNAMGSTAALQDPDSRSLME
                     DFPLLPDSLKPPVSATNLAQYAYAIDADTGPENLQLVQSHVGDLADSGDPRGWVNGQL
                     GTAQRAAQVFTEIDGMDGTSWYHPLRLSIDAGAINNGIATPAQQVYGIKTTMGKRVHL
                     PMYSFDTSLGDGRVKRATDALAAQSKVPKSWVRTVVRKETYAHIDPLSATPSKNDFIK
                     TLVPFLKKQVK"
     gene            complement(2565..3014)
                     /locus_tag="PLV77_02260"
     CDS             complement(2565..3014)
                     /locus_tag="PLV77_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14703.1"
                     /translation="MSSIEDAIQQLETSASKLKQLSVEESRAIREAVRVATREATAKV
                     KAEYKDKKDKARKEARDAEKSIKDAQSRIQKALGSEKGGSTGGKRAKRGEREAQFVAY
                     VKENPDSKLADIARGIGVQNSAANGLAKKAVAAGKVKKSAQKTYKAA"
     gene            3211..>3578
                     /locus_tag="PLV77_02265"
     CDS             3211..>3578
                     /locus_tag="PLV77_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC14704.1"
                     /translation="MPPSRFRSSRNPIPHERAFWAMAAIAVTFPAISAVPSPLYSLYQ
                     DMWGFSDAVLTEVFAVYVVALLCSLLVFGSLSDHLGRRPVLLGAIAFEALSLLLFLLS
                     GGVAMLALARVIQGLATGVAL"
BASE COUNT          530 a         1197 c         1118 g          733 t
ORIGIN      
        1 ggactctgcc attccgacct ttcggtgatc accggccagc ggccgcgggt gatgccgatg
       61 gcgctcggcc acgaggcggc cggtgaagtc gtcgcgaccg gcccgggcga ccacggcttc
      121 gtcgagggcg accacgtggt gctcgccttc gtcccggcct gcggcgagtg ccgcttctgc
      181 gcgcaggagc ggcccgcgct ctgcgttccc ggcggtgaag ccaacgtcga gggaaccctg
      241 ctgggtggtc acatccgcct gttcgaggag agcggcaccg aggtccacca tcacctcggc
      301 gtttccgccc tttccgacca cgccgtggtc tcggcccgct cggcggtcaa ggttccggat
      361 gaccttccct ttgacatcgc ggcccttttc ggctgcgcgg tcctgaccgg tgccggcgcg
      421 gtgctcaaca cgggtgccgc cgctttgccg gcccgatcgg tcgccgtctt cggactgggc
      481 ggggtcggcc tggcggcgct gcttgccgcg aagtcacaaa acccggaaac cctggtcgcg
      541 gttgacaccg tgccggccaa gctcgagctg gcccgcgagc tgggagcgga cctgaccatt
      601 caggccgggc ccgatctggt cgattcgatc cgcgaagcga ccggcggcgg cgtcgacctc
      661 gcgatcgaaa cggtcggcaa cgagaacgtg ctggccgatg cctacgcggc aaccggccgc
      721 ggcggcacca cggtcaccgc cggcctgccc caccccagcc gcaagctgga gatcccggcg
      781 atctcactgg tcgccgagga gcggaccctg aaaggctcgt acctgggctc ctgcgtgccc
      841 cgccgggaca tccccgcgtt cattcgcatg taccgggacg gtgagttgcc ggtcgagaag
      901 ctgctcagcc accggctcac accggatcag atcaacgagg gcttcgaccg cctcgcccgg
      961 ggcgaggcgg tccgccagat cgtcatcttc gactgaggct gcaactggat tgaagttatt
     1021 cgctctatgt gcggataact tcaatccaga tggcggcccc tacttgacct gcttcttcag
     1081 gaacgggacc agcgtcttga tgaagtcgtt cttcgacggg gtggcgctga gcgggtcgat
     1141 gtgggcgtag gtctctttcc ggaccaccgt ccgcacccag ctcttcggca cctttgactg
     1201 ggccgcaagc gcatcggtcg cccgcttgac ccggccgtcg ccgagcgagg tgtcaaacga
     1261 gtacatcggc aggtgaaccc gcttgcccat ggtcgtcttg attccgtaga cctgctgggc
     1321 cggggtggcg atcccgttgt tgatcgcacc ggcgtcgatg ctcagccgca gcgggtgata
     1381 ccaggaggtg ccgtccatgc cgtcgatctc ggtgaagacc tgggcggccc gctgggcggt
     1441 gccgagctgg ccgttgaccc agccccgggg gtcaccggaa tccgcgaggt caccgacgtg
     1501 gctctggacc agctgcagat tctccgggcc ggtgtcggcg tcgatcgcgt atgcgtactg
     1561 ggccaggttg gtcgccgaaa cgggcggctt cagcgaatcc gggagcagcg ggaaatcctc
     1621 catcagcgag cgcgaatccg gatcctgaag cgccgcggtc gaacccatcg cgttgaagac
     1681 ccctgccgcc cagggcagcg gcggcaccag cgtgatgaag ggcgaggcgc tttcggcgtt
     1741 caagctgtcg aggttggcct gcgcttcccc ggccgtgggc aggtccgcgg cctcgcgggc
     1801 cccggcaccg tcgatgaaga ccaggccgga aagtcccttg gcgcccggcg ttccgttgaa
     1861 attccaggtc gcgtaggcgg tgacgatcga accgcccagc gagtggccgc caagcacgac
     1921 cttgcggccg cctttgcggg ccatgttcac gaccttgcga agatcgtgaa cgactatgct
     1981 cagaccccac ttcttcgcgt actgggtttc cgcggtggtc ttgccctcga aatgaggctg
     2041 aaccgagccg tccaggatcc agccgaggta gtagtcgagc atctcctcgt tggtcgcatc
     2101 acccttgagg tacttctcca gcatcgagtg gtcttcgagc aggttctcgc gacgctcgat
     2161 cgaccagacc tgccagccct tgagttgacc gaccagggcg ttaccgaggg ggcggaagtt
     2221 ggtggcaccg gccgaagtgc cgggctcgag gaggaggatc ttctttgcct tggccggccc
     2281 ctgctttacg accttgaact tgttgtactt cgccggtgtg cccggcgcgg gcgtgccctg
     2341 aacggtcatt acgttcagct tggccgaagc gcttcccgac atgaaaatgc ctagcgccgc
     2401 cactgttgcc agaaaggcca gcgatcccct gcgaacaacg ttcatttatc tctcctcttg
     2461 ctcgtcacgg ccctcccggg agccgcatta ccggtcgatt ctaagggctg aaacacgaaa
     2521 aagggcccgc cggagcgggc ccttcaaaac atctctgaaa gtgatcaggc ggccttgtag
     2581 gtcttctggg cgctcttctt caccttgccg gcggcgactg ccttcttggc cagcccgttg
     2641 gcggcgctgt tctgcacacc gattccccgc gcgatgtcgg cgagcttcga atcggggttc
     2701 tccttcacgt atgcgacgaa ctgggcttcc cgctcgccgc gcttggcacg cttgccgccg
     2761 gtcgacccgc ccttttccga accaagagct ttctggatcc tggactgggc gtccttgatt
     2821 gacttctcgg cgtccctggc ttccttgcgg gccttgtcct tcttgtcctt gtactcggcc
     2881 tttaccttcg cggttgcttc cctggttgca accctgaccg cttcgcgaat cgcacggctt
     2941 tcttcaaccg aaagctgctt gagcttcgaa gctgaagttt caagttgttg aattgcgtct
     3001 tcaatgctgg acatagtctc tcctgttggc gattgtcatg tgcctgccag acatcgtcat
     3061 gagaccggca ttcatgacta taaccaaacg acgggtttca aatgtcaatt ccggtccgta
     3121 acgaaatgtc gtcttccgga tgtcaggcaa gtgcgcggtt gctcaagagg tgccgttggt
     3181 accttgcctg tctccccctg actcgccctg atgcctcctt cacgcttccg ttcatcccgc
     3241 aatccgatcc cccacgaacg cgccttctgg gcgatggcgg cgatcgccgt gaccttcccc
     3301 gcgatctcgg cggtgccttc cccgctctac tccctctacc aggacatgtg gggcttctcc
     3361 gacgcggtcc tgaccgaggt cttcgccgta tacgtggtcg cgctgctctg ctccctgctc
     3421 gtcttcgggt cactctccga tcacctcggg aggcgcccgg tgctgctcgg agcgattgcc
     3481 ttcgaggccc tttccctcct gctgttcctg ctttccggcg gagtcgcgat gctggccctg
     3541 gccagggtaa tccagggtct cgccaccggg gtggcgct
//
LOCUS       DAOQMF010000087         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13630_length_3578_cov_4.025263, whole genome shotgun sequence.
ACCESSION   DAOQMF010000087 DAOQMF010000000
VERSION     DAOQMF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13630_length_3578_cov_4.025263"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="PLV77_02270"
     CDS             complement(<1..1479)
                     /locus_tag="PLV77_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC14705.1"
                     /translation="MEASALNHAARRGLLARKSPLLKLQGDEKLVAMVRAGNSGAFDT
                     IVDRYQARLLGFCRQMIGSTEDAEDVLQEVFVNAYKAMMADNREINLRPWLYRIARNR
                     SLNHLRKPRAVAQESMDMVPMVDAASTAEKVHNREEFRQLLQDVTKLPETQRAALLLR
                     EIDALSYEEIAEAMDTTVPSVKSLLVRARISLAEASQARQLTCGEVRVELAEAAEGLA
                     KASGPVRRHVRDCEECAAFRKQLRTDNKALAAMMPIGVLAGLKATIAAKLGIGGSSAG
                     AGAGAAGTSAGAGAAGAAGAAGTGAAISAGTAASGIGAVTSAVGAKALAGVVTAAVIT
                     AGAVEVKEKVSTSPVDTASPTTSKTIADSNPLQPVHVATPVNHSAPADVELDKASAEA
                     PVTEPVDTTVPSQETDTTAVPVDHPAAETSAMPTEPEIPANPATSEGGDGLEGHTTYP
                     PDEIVNEGPATVPSEGSTDTSGQIPGTAPSTPVVTDPATPVVP"
     gene            1657..1741
                     /locus_tag="PLV77_02275"
     tRNA            1657..1741
                     /locus_tag="PLV77_02275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1690..1692,aa:Leu,seq:taa)
     gene            1795..3201
                     /locus_tag="PLV77_02280"
     CDS             1795..3201
                     /locus_tag="PLV77_02280"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC14706.1"
                     /translation="MTKERPHLGWVRFITILAGFITVLAILSTWVDRQVFDTQEWGDT
                     SLKLLQNPEIQKQVATYAVDELYASVDVQGELEKNLNDINKNLGDTLAGPLAGAARQG
                     ADSLALRALSNDKVQSAWKSANVAAHTTLINILEDKGTYTSTAGGQVELKLQPLIIEI
                     ADQVGLGDQARDKVPDSVGNIEIVDSDQLAQVQTVAKLIHGLALVTALLALVLIVLAV
                     YLSPGYRWLTLLWLAVALVLAALIILIIRSVAGNVLIPDLADPDIQPAAQAAWDIITE
                     LLKSVAWTVIWFAVALLVVAWVISPVKAAGKVREFLAVPFGQYPIPVFSLLGLAAFIF
                     LIMGAGDGREFIVRLAVVLMAGVGFWLFRRQVMLEYPDADFASLREFGERTRDRAKGA
                     WADRPKNISMPKIGGKDGDAAGQSGGEAETAVLPAQPATGEGSRLDQLEKLGKLKEAG
                     VLDDEEFAAEKKRILGGE"
     gene            complement(3229..>3578)
                     /locus_tag="PLV77_02285"
     CDS             complement(3229..>3578)
                     /locus_tag="PLV77_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14707.1"
                     /translation="LEGVELPAVDRAVAVAGTPSSLAAIDLELEPYDPKAVEGHRLPL
                     RTVQWWLSRLASMPLEDRKQVPGLHPDRAQAIVAGVVILIEIMRNFDLLEIEVSEHDI
                     LYGCAITAAASAA"
BASE COUNT          576 a         1126 c         1245 g          631 t
ORIGIN      
        1 cggcaccacc ggggtggccg gatcggtcac caccggggtc gaaggcgcgg tacccgggat
       61 ctgccccgag gtgtcggtcg aaccttcgct cggcacggtc gccgggcctt cgttcacgat
      121 ctcgtcgggc gggtaggtgg tgtgcccctc gagaccgtcg ccaccttcag aggttgcggg
      181 gttggccggg atctccggtt cggtcggcat ggccgaggtc tcggcggccg gatggtcgac
      241 cgggacggcc gttgtatcgg tttcctggct ggggaccgtg gtatcaaccg gctcggtcac
      301 cggcgcctcc gccgaagcct tgtcgagctc gacgtcggcc ggcgcggaat ggttcaccgg
      361 agtggcgacg tgaaccggct ggagcggatt gctgtcagcg atcgtcttcg aggtcgtcgg
      421 tgaggcagtg tcaaccgggc tggtcgaaac cttttccttg acttccacgg cgccggccgt
      481 gatgacggcg gcggtgacga caccggccag ggccttggcg ccgaccgcgc tggttacggc
      541 accgattccg gaggcggcgg tgccggcgct gatcgcggca ccggtaccgg cggcaccagc
      601 ggcaccggca gcaccggcgc cagcggaagt accggctgca ccggcacccg caccggcgga
      661 ggatccaccg attccgagct tggcggcgat cgtggccttg aggccggcca gcacgccgat
      721 cggcatcatc gcggcgagag ccttgttgtc agttcgaagc tgcttgcgga aggcggcgca
      781 ttcctcgcag tcgcgcacgt gccggcggac cgggccggaa gccttggcga ggccttcggc
      841 ggcctcggcc agctcgaccc ggacttcacc acaggtcagc tgacgggcct ggctcgcttc
      901 ggccagggag atgcgggccc ggaccagaag cgacttgacc gagggaacgg tcgtgtccat
      961 cgcttcggcg atctcctcgt aggagagggc gtcgatctcg cgcagaagca gggcggcgcg
     1021 ctgggtctcg gggagcttgg tcacgtcctg caagagctga cggaactcct cgcggttgtg
     1081 gaccttctcc gcggtcgagg cggcgtccac catcgggacc atgtccatcg actcctgggc
     1141 gacggcgcgc ggcttgcgca gatggttcaa agagcggtta cgggcgatcc ggtacagcca
     1201 gggccgcagg ttgatctccc ggttgtcggc catcatcgcc ttgtaggcgt tgacgaagac
     1261 ttcctgaagg acatcctctg cgtcctcggt cgagccgatc atctggcggc agaagccgag
     1321 cagacgggcc tggtaccggt cgacgatcgt gtcgaaggct cccgagttgc cggcccgcac
     1381 catggcgacc agcttttcgt cgccttgaag cttcagcagg ggcgacttcc tggcgagaag
     1441 acctcgtcgt gccgcgtgat ttagtgctga tgcttccaag ggactccttt tgactgggga
     1501 ccttgcggtc cgggtttcct cctgtgctta cggaaacacc gtacccagcc aaatgtagcg
     1561 gtctctaaac gggaatattc cggtttctaa agattgggcc tgcctcccat tctacccctg
     1621 ttccccgccc acaaccctaa gttctaggct taaggggccc gggtggcgga atggtagacg
     1681 cagcggcctt aaaagccgca gtcccgcaag gggcgtgcgg gttcgagtcc cgctccgggt
     1741 acttggctag gcttgcggca tattggcgcg tcagatcaga aggggagtag tgctttgacc
     1801 aaagagcggc cgcatctcgg gtgggtcagg ttcatcacga tcctggccgg tttcattacc
     1861 gtgctggcga tcctttcgac ctgggtcgac cgccaggtct tcgacaccca ggagtggggc
     1921 gacacctcgc tgaagctgct gcagaacccg gagatccaga agcaggtcgc gacctacgcg
     1981 gtcgatgagc tctacgccag cgtcgacgtc cagggtgagc tggaaaagaa cctcaatgac
     2041 atcaacaaga atcttggcga caccctggcc ggaccgctgg ccggagcggc caggcagggc
     2101 gccgacagcc tcgccctgag agcgctgagc aacgacaagg tgcagtcggc ctggaagtcg
     2161 gccaatgtcg cggcgcatac gaccctgatc aacatcctcg aggacaaggg cacctacacc
     2221 agcaccgccg gtgggcaggt cgaactcaaa cttcagccgc tgatcatcga gatcgccgac
     2281 caggtcggcc tcggtgacca ggcgagggac aaagtccccg attcggtcgg caacatcgag
     2341 atcgtcgatt cggaccagct ggcccaggtc cagaccgtgg cgaagctgat ccacggcctg
     2401 gccctggtca ccgcgctgct tgcgctggtg ctgatcgtgc tggccgtcta cctctcgccg
     2461 ggataccgct ggctgaccct gctctggctg gccgtcgcgc tggtccttgc ggccctgatc
     2521 atcctgatca tccgttcggt ggcgggcaac gtgctgatcc ccgacctggc cgacccggat
     2581 atccagccgg ccgcacaggc cgcctgggac atcatcaccg agttgctgaa gtcggtggcc
     2641 tggaccgtga tctggttcgc ggtggccctg ctggtcgtcg cctgggtcat ttccccggtc
     2701 aaggcggccg ggaaggtccg tgagtttctg gcggtgccct tcggccagta tccgattccg
     2761 gtcttcagcc tgctcggtct ggctgccttc atcttcctga tcatgggtgc gggtgacggc
     2821 cgggagttca tcgtccggct ggccgtggtg ctgatggccg gagtcggttt ctggctcttc
     2881 cggcgtcagg tgatgctgga gtaccccgat gcggacttcg cttccctgcg cgaattcgga
     2941 gagcggaccc gtgaccgggc gaagggagcc tgggccgacc ggccgaagaa catctcgatg
     3001 ccgaagatcg gcggaaagga cggggatgcc gccggtcaga gcggtggtga ggccgagacc
     3061 gcggtcctgc ccgcccagcc tgccacgggg gaaggcagcc ggctcgacca gctcgaaaag
     3121 ctcggcaagc tcaaggaagc cggtgtgctc gatgacgagg agttcgcggc cgagaagaag
     3181 cggatcctcg gcggggagtg agcggcggcg tcgggccgcc gaccgggttc aggcggccga
     3241 ggcggctgcg gtgatcgcgc agccgtagag gatgtcgtgc tcggagacct cgatctccag
     3301 caggtcgaag ttgcgcatga tctcgatcag gatcaccacc ccggcgacga tcgcctgggc
     3361 acggtccggg tgaagccccg gcacctgctt gcgatcctcc agcggcatcg aggcgaggcg
     3421 ggaaagccac cactggacgg ttctgagcgg aagccggtga ccttcgaccg ctttcgggtc
     3481 gtaaggctcg agctcgagat cgatcgcggc cagggacgag ggagtgccgg cgacggcgac
     3541 ggcgcggtcg accgccggca actcgactcc ttcaagcg
//
LOCUS       DAOQMF010000088         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13671_length_3571_cov_4.265358, whole genome shotgun sequence.
ACCESSION   DAOQMF010000088 DAOQMF010000000
VERSION     DAOQMF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13671_length_3571_cov_4.265358"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..647)
                     /locus_tag="PLV77_02290"
     CDS             complement(195..647)
                     /locus_tag="PLV77_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14708.1"
                     /translation="MKTVSTKPGAVQIDLLRNAIAFVSTNWDEDLAAALDGREGMSKL
                     RALASWSLQTNNYDDAIRRLWIEALAGTAPEATEEIAAAKEQAQSEYQFVLAVTKEAG
                     QDKQLADALFLAFRGYYLSSIEDPEHWPAERAQEAVLKVLDMLDKRAR"
     gene            complement(644..1860)
                     /locus_tag="PLV77_02295"
     CDS             complement(join(644..1572,1572..1860))
                     /locus_tag="PLV77_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612098.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNC14709.1"
                     /translation="MSRSKRYPREFRERSVALVLEHQGEHRSQWACIQSVAAKSGMTP
                     ETLRRWVRQAEVDSGGRAGVSTDERKRIRELERENRELRRANEILKAASAFFGAGARP
                     ASTEEMTAFIEEHREKFGIEPICQIMDFAPSTYYAALKRRENPSARDLRDQELIPEIR
                     RVHRENLGVYGARKTWLQLKREGIMVPRCQVERLMREEGLQGVVRGRKAPRTTFPGGQ
                     AKRALDLVDRKFEAQAPNRLWVADFTYVFTWEKLCFAAFVIDVFSRRIVGWRVADSMR
                     TELVLDAFEMALWSRGHEDLPVGEGLVVHSDAGSQYTSFAFTRRLIDAGVDASIGSVG
                     DAYDNALAESTIGLYKTELINKDGPWRTRSDVELATLTWVDWFNNRRLHGACGDLPPI
                     EFEQQHQAASITQ"
     misc_feature    complement(1466..1582)
                     /locus_tag="PLV77_02295"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1920..>3571
                     /gene="asnB"
                     /locus_tag="PLV77_02300"
     CDS             1920..>3571
                     /gene="asnB"
                     /locus_tag="PLV77_02300"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNC14710.1"
                     /translation="MRETLHHRGPDGEGLFITEDRKVGLAHRRLSIVDLEGGAQPMFG
                     ARGEALVFNGEIYNYPRLRLELEADGVEFSTTCDTEVILHLYAAHGRDCVDHLEGMFA
                     FAIWDPNAEELFFARDRIGEKPLYWTCVDGRFIFGSEIKAILAHPSVKPEVDEDSIGP
                     YLTNLVTSSPQTLYRNIQKVPPGVSGICDSGGVRTHQYASVAGSRTFADHSPDLAAKT
                     VRDLLDRSIHDRLMSDVPVGVLLSGGLDSTTIVAMLRDQAAGLATFSVGFNGDVAIDE
                     RDEARRVARHFGTDHHEVEIDGDDARRFLTGLVHHQDEPLADPVCLPLNFVCQLAAEN
                     GIKVVMAGEGADELFWGYPGYSRVLAHETALRTLLASPTLIRKSIAALMPRRSKPMLR
                     ELMEGIARGRPLPLHMPLGLTRRQRNEMLDRDQPAYSGWAPNEAENIAGESAIETLLW
                     DTQEYEFQLRLPELLLMRIDRFSMANSVEARVPFLAPDLVDYVYSLPLQQKWREGTTK
                     VVMRKGIGDLVPDWVLNRPKRGFGAPVEEWFAGDLRTVFEQL"
BASE COUNT          673 a         1095 c         1043 g          760 t
ORIGIN      
        1 cctcgaaaac gaaggggtgc cgatcatcac ggttgaggcc gtggtcgacg ggatcgtcga
       61 tctgatggcc tccgatgaaa gcggcgtctg ccacttcgtc caggccggca tggaccccca
      121 ggagttccgc ttccgcaaca tccccggccc gagggccgcc gaggcctgag ccggcctccg
      181 cttcagccgc gtgactaccg ggcgcgtttg tccagcatgt ccaggacttt gagcaccgcc
      241 tcctgcgctc tctcggcagg ccagtgttcc gggtcttcta tcgatgagag gtaatacccc
      301 ctgaacgcaa ggaaaagcgc atcggccaat tgcttgtctt gtcccgcttc ttttgtcacc
      361 gctaggacga attggtactc gctttgcgcc tgttccttcg cagcggcgat ttcctcggtt
      421 gcttccgggg cggtgccggc gagagcctct atccatagcc ggcggatcgc gtcgtcgtag
      481 ttgttggtct gaagcgacca gcttgcgaga gctctgagct tggacatgcc ttcgcgtcca
      541 tcgagagccg cggcaagatc ttcatcccag ttggttgaca cgaacgcaat cgcgtttctc
      601 agcaggtcga tttgaaccgc cccgggtttt gtggagacgg ttttcattgg gtgattgatg
      661 ctgcttggtg ttgctgttcg aactctatgg gtggcaggtc tccgcaggct ccatggagcc
      721 tgcggttgtt gaaccagtca acccaggtca gggttgccag ttcaacgtct gagcgggtcc
      781 gccagggtcc gtccttgttg atcagctcgg ttttgtagag accgatggtg gattccgcca
      841 gcgcgttgtc gtaggcgtcc ccgaccgatc cgattgaagc gtcgacgccg gcatcgatga
      901 gccggcgagt gaacgcaaac gatgtgtatt ggctaccggc gtcggagtga accacgagtc
      961 cctcgccgac cggaaggtct tcgtggcctc tggaccagag cgccatctcg aacgcatcga
     1021 gaaccagttc ggttcgcatt gagtcagcga ccctccagcc aacgatccgg cgggaaaaca
     1081 cgtcgatcac gaacgccgca aagcaaagct tctcccacgt gaacacgtag gtgaaatcag
     1141 cgacccagag ccggttcggt gcctgggctt cgaacttccg gtcaaccaga tccagggccc
     1201 gtttagcctg ccctccagga aaggttgttc tcggggcttt ccggcccctg accacgcctt
     1261 gaagaccttc ctcgcgcatc aggcgttcca cctggcagcg cggcaccatg atgccctccc
     1321 gtttcagctg aagccaggtt ttccttgccc cgtagacacc aaggttttcc cggtggaccc
     1381 tccggatctc cgggatcaac tcctggtcac gaagatctct cgctgacggg ttctctcttc
     1441 gtttgagtgc cgcatagtag gtggacgggg cgaaatccat gatctggcag atcggctcga
     1501 tcccgaactt ctcgcgatgc tcctcgatga acgcggtcat ctcttcggtg gatgcgggtc
     1561 gagctcccgc gcgaaaaaag ccgaagcggc cttcaggatc tcgtttgctc tcctgagctc
     1621 ccggttttcc ctttcgagtt cacgaatgcg ttttcgttca tcggtgctga ccccggcccg
     1681 gccaccggaa tcaacctccg cctgcctgac ccaacgcctc aacgtctccg gggtcatccc
     1741 cgacttcgcc gcgaccgact gaatacacgc ccactgcgag cgatgctcgc cctggtgctc
     1801 gagcaccagg gcaacagaac gctcccggaa ctcccgcgga tacctcttcg acctagacat
     1861 ttgaccgatc ctctcaaagc aattggcctc caacaaaccc ggggcggttc atagtgagga
     1921 tgagggagac ccttcaccat cgtgggcccg atggtgaagg tcttttcatc actgaggacc
     1981 gcaaagtggg gcttgcccac cggaggctct caatcgtaga cctcgaaggc ggcgcacaac
     2041 cgatgttcgg ggcccggggt gaagcactcg tgttcaacgg cgagatctac aactatccgc
     2101 gtttgcgact cgagttggaa gccgacgggg tggagttctc cacaacatgc gacaccgagg
     2161 tgatccttca cctatacgcg gcacatggtc gtgactgtgt cgaccacctg gaaggcatgt
     2221 tcgctttcgc gatctgggac ccaaacgcgg aagagctctt tttcgcgcgc gaccggatcg
     2281 gagaaaagcc gctttactgg acctgtgtgg atggccggtt catcttcggc tccgaaatca
     2341 aagccatcct ggcgcatcca tccgtcaagc cagaggttga cgaagactcg atcggtccgt
     2401 atctgacgaa tctcgtcaca tcaagtcccc aaacccttta caggaacatc cagaaggttc
     2461 cgcccggagt gtccgggatt tgcgattcgg gtggcgtacg cacccatcag tatgcctcgg
     2521 ttgccggcag ccggaccttt gcagatcact cccctgacct ggccgcgaaa acggtccggg
     2581 atctactcga ccgctcaatc catgatcgcc tgatgagtga cgtcccggtc ggagtcctcc
     2641 tgagcggagg tctcgattcg acgacgatcg tggccatgtt gagagaccag gcggccggac
     2701 tggccacatt ctcggtcggt ttcaacggtg acgttgcgat cgatgagcga gacgaggccc
     2761 gccgggtggc ccgccatttc ggaaccgatc atcacgaggt cgaaattgac ggcgatgatg
     2821 cccggcgctt tctgaccggt ctggtccacc atcaggatga acctctcgcc gatccggtct
     2881 gcttgccgct gaactttgtc tgccagcttg ccgctgagaa tggaatcaag gtcgtgatgg
     2941 ccggcgaagg cgcagatgaa cttttttggg gataccccgg atactcgaga gtcctggctc
     3001 acgaaacggc attgcgaacc ctgcttgcct cgccgaccct gattcgaaag tcaatcgcag
     3061 ctctgatgcc acgccgttcc aagcccatgc tccgtgaact tatggaggga atcgccaggg
     3121 gacggccgct tcctcttcac atgccattgg gtttgacccg aagacagcgc aacgaaatgc
     3181 tggaccgcga tcagcctgcg tattccggct gggcgccgaa cgaagcggag aacatcgctg
     3241 gcgagagcgc gatcgaaacg cttctctggg atacccagga atacgagttc caactccgcc
     3301 tgccagaact tcttctgatg cggatcgatc gcttttccat ggccaacagc gttgaggctc
     3361 gggtgccgtt cctggctcct gatcttgtcg attacgttta ctccctgccg cttcagcaga
     3421 agtggcgaga aggcacgacg aaggtcgtga tgcgaaaggg catcggggat ctggtaccgg
     3481 attgggtgct gaaccgcccc aagcgtggct tcggggcccc ggtggaggaa tggttcgccg
     3541 gcgacctccg aacggttttc gagcaactga t
//
LOCUS       DAOQMF010000089         3555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13767_length_3555_cov_4.221714, whole genome shotgun sequence.
ACCESSION   DAOQMF010000089 DAOQMF010000000
VERSION     DAOQMF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13767_length_3555_cov_4.221714"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLV77_02305"
     CDS             complement(<1..213)
                     /locus_tag="PLV77_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14711.1"
                     /translation="MNHSSATLRRPVLLAIALVTALTMLAAWSASSRAAVICVPDAGV
                     SPDCQTSFATIQPALDSAKNSGGNDEI"
     gene            complement(238..1527)
                     /locus_tag="PLV77_02310"
     CDS             complement(238..1527)
                     /locus_tag="PLV77_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14712.1"
                     /translation="MDGATLKDSAVRLYSSNVNDAVDVYGSGNVVDSTLTAYRGVYQM
                     NSTGTVEIKRSALIPIDGGAVTEGGTMNIRDSVIINLNGGRGVFVDSYRGPAHVLIDG
                     STILGAPGSTGAEVHARRDQDDNPGTTSFKVNNSIIYGQDFAIVYHEYFAEDTLTVDV
                     DSSAYDSSRVVEQPGAGTPQFTTKDPVELNGVDPMFADPDHGDYSPDAGSPLIDAGQA
                     ALPPVGSLAYDRHPRACDGNDDGTIRRDIGAWEYRSDPSDDCTYSKATINGPRGAATA
                     RSRSFTFKASKPGSTYLCSLDGAAFAACTTPYTASGLAVGNHSLKVKAKDSYGNVQTP
                     PTEKSFKVEAADKTAPKVIGVKAPKQTRAATVKVSFKSNEKGSTFKCRLGKGKWKSCK
                     SPWKTPKLRKGRNSISIQATDRAGNRSKVKTVAVKRK"
     gene            complement(1533..2048)
                     /locus_tag="PLV77_02315"
     CDS             complement(1533..2048)
                     /locus_tag="PLV77_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14713.1"
                     /translation="MKRALIFMAIGLASIAAFGATQAQANTYCAELPADASCDQNFTG
                     TGTSIQAAVEAAADNPGPDSVKVGPGNFVMPSPVSLSSPGDANKLTLTGSGPSTVLSG
                     DDPQLPDINFSGGTGSSINDLTVSISDDASNEYKVGLALGAGATGSRLTVTFASHLVN
                     GDPSPGKKLLA"
     gene            2120..>3555
                     /locus_tag="PLV77_02320"
     CDS             2120..>3555
                     /locus_tag="PLV77_02320"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNC14714.1"
                     /translation="MDDAAKKKLEETTRWEPGETEPRIFAEWLEGGYFHPEATGTADQ
                     NYSVAIPPPNVTGVLHMGHALNGSMQDALVRMNRMSGRNTLWILGMDHAGIATQTVVE
                     KRLKSEGISRHEIGREAFTERVWEWKEEFGGSIREQYKRLGASVDYERERFTLDDGYA
                     RAVRKVFTSLYEKGYIYRDHYMVNWDPGSQSAISDLEVVNEEVTDHLYSVDYPVEGSD
                     RVLTVATVRPETMMADTAVAVNPKDERYSDLIGQHCVLPLVGRRLPIIADDHVDVEFG
                     TGALKITPGHDVNDFEIGRRHGLEEISVIGEDGRMTAEAGERFEGMTVAEAQVEVVKA
                     LKEEGALSGEEEYVHSVPFSERSGERIEPLISLQWFCQMDELARPAIEAVEDGRVNIA
                     PGQWKRVYLDWMREIRPWCVSRQLWWGHRIPVWYRGNETYVGETEPEGEGWVQEEDVL
                     DTWFSSAIWPFATLGWPDETDELKARSP"
BASE COUNT          607 a         1065 c         1215 g          668 t
ORIGIN      
        1 gatttcatcg ttgccgccgc tgttcttggc tgagtcgagg gctggctgga tggttgcgaa
       61 gctcgtctgg caatccgggc tgacccctgc gtccgggacg cagatcaccg cggccctgga
      121 gctggcgctc caggcggcca gcatggtgag cgcagtgacc agggcgattg cgaggagaac
      181 gggacgacga agagtggctg aagaatggtt cattggaatg gagataggaa cctgtgttta
      241 tttgcgcttg acggcgactg tctttacctt cgacctgttg cctgcccggt ctgtggcctg
      301 aatgctgatc gagttcctgc ccttgcggag cttcggggtc ttccagggtg acttgcagct
      361 tttccacttg cccttgccga ggcggcactt gaaggtcgat cccttctcgt tcgacttgaa
      421 gctgaccttg accgtggcgg ccctggtctg tttcggggcc ttgaccccga tcaccttcgg
      481 ggcggtcttg tcggccgcct cgaccttgaa cgacttttcg gtcggaggcg tctggacgtt
      541 gccgtaagag tctttcgcct tgaccttcag gctgtgattg ccgaccgcca gtcccgaagc
      601 cgtgtacgga gtggtgcagg ccgcgaatgc ggcgccgtcc agcgagcaga gataggtcga
      661 gccgggctta gacgccttga aggtgaagga ccggctcctt gcggtcgcgg ccccccgggg
      721 gccgttgatg gttgctttcg agtaggtgca gtcgtcgctc gggtcggacc ggtactccca
      781 ggccccgatg tcccggcgga tggttccgtc gtcgtttcca tcgcaggcgc gggggtggcg
      841 gtcgtaggca agcgaaccaa caggaggaag ggcggcctgg ccggcgtcga tcagcggcga
      901 cccggcgtca ggcgagtagt cgccgtggtc ggggtcggcg aacatcgggt cgaccccgtt
      961 cagttcgacc ggatccttgg tcgtgaactg gggcgtgccg gcgccgggtt gttcgaccac
     1021 cctggaggag tcgtaggccg aggaatccac gtcgacggtc agggtgtcct cggcgaagta
     1081 ctcgtgatag acgatggcga agtcctgccc gtagatgatc gagttgttga ccttgaaact
     1141 ggtggttccg gggttatcgt cttgatcccg tcgggcgtgg acctccgctc cggtcgagcc
     1201 gggtgcgccc aggatcgtcg aaccgtcgat cagcacgtga gccgggcctc tgtagctgtc
     1261 cacgaagacg ccgcgcccgc cgtttagatt gatgatcacc gagtcacgaa tgttcatcgt
     1321 gccgccttcg gtcacggcgc caccgtcgat cgggatcagg gccgatctct tgatctccac
     1381 cgtgccggtt gaattcatct ggtagacccc gcggtaggcg gtcagcgtcg aatccaccac
     1441 gttgccggac ccatacacgt caactgcatc gttgacgttg ctgctgtaga gcctgacggc
     1501 cgagtccttg agggtggccc cgtccaccag cgtcacgcca gtagtttttt gccagggctc
     1561 gggtctccgt tgacgagatg cgaggcaaag gtgacggtca gcctgcttcc ggtcgcgccg
     1621 gcacccagcg caagaccgac cttgtattcg ttgcttgcgt cgtcggaaat gctgaccgtg
     1681 aggtcgttga tgctcgagcc ggtgccgccg ctgaaattga tgtcgggaag ctgcgggtcg
     1741 tcgccggaaa ggaccgtgct gggtccggaa ccggtcaggg tcagcttgtt ggcgtccccg
     1801 gggctcgaga gggagaccgg acttggcatc acgaagttgc cggggccgac cttgaccgag
     1861 tccggccccg ggttgtcggc ggcggcctcg accgcggcct ggatcgaggt gcccgttccg
     1921 gtgaagttct ggtcgcagct cgcgtcagca ggaagctcgg cgcagtaggt gtttgcctgc
     1981 gcctgggttg ccccgaatgc cgcgattgac gcaaggccga tcgccatgaa gatgagtgcc
     2041 cgtttcatca aaccttcccg gtaagacagt gaagtaccga acaccctaac ccggtggccc
     2101 gtcaataaga tcggattcct tggacgacgc ggcaaaaaag aagctggaag agaccacccg
     2161 ctgggagccc ggcgagaccg agccgcggat ctttgccgag tggcttgaag gcggctactt
     2221 ccatcccgag gcgaccggca ccgccgacca gaactactcg gtcgcgatcc cgccgccgaa
     2281 cgtgaccggc gtgctccaca tgggtcacgc cctcaacggc tcgatgcagg acgccctggt
     2341 ccggatgaac cggatgagcg gcaggaacac gctctggatc ctcggcatgg accatgcggg
     2401 catcgcgacc cagacggtgg tcgagaagcg gctcaagtcg gaggggatca gtcgccacga
     2461 gatcggccgc gaagccttca ccgagcgggt ctgggagtgg aaggaagagt tcggcggttc
     2521 gatccgcgag cagtacaagc ggctgggtgc ctcggtcgat tacgagcgcg aacgcttcac
     2581 gctcgatgac ggatacgccc gtgcggtccg caaggtcttc acctcgcttt acgagaaggg
     2641 ctacatctac cgcgaccatt acatggtcaa ctgggatccc ggttcgcagt cggcgatctc
     2701 cgatctcgaa gtcgtaaacg aggaagttac ggatcacctc tattcggtcg attacccggt
     2761 cgagggaagc gaccgggtgc tgaccgtcgc caccgtccgt ccggagacga tgatggccga
     2821 caccgcggtc gccgtgaatc cgaaggacga gcgctacagc gacctgatcg gtcagcactg
     2881 cgttctgccc ctggtcggac gccggctgcc gatcattgcc gacgaccacg ttgacgtcga
     2941 gttcggcacc ggcgcgctca agatcacccc cggccacgac gtcaacgact tcgagatcgg
     3001 ccgccgtcac gggctggaag agatctcggt gatcggcgag gacggccgca tgaccgccga
     3061 agccggcgaa cggttcgaag gcatgaccgt ggccgaagcc caggtcgagg tggtcaaggc
     3121 gctgaaggaa gaaggggccc tgagcgggga ggaggagtac gtccattcgg ttcccttctc
     3181 cgagcgctcc ggcgagcgga tcgagccgct gatctcactt cagtggttct gccagatgga
     3241 cgagctggcc aggccggcga tcgaagcggt cgaggacggc cgcgtgaaca tcgcccccgg
     3301 gcagtggaag cgggtctacc tggactggat gcgggagatc cgcccctggt gcgtttcccg
     3361 ccagctctgg tggggtcacc ggatcccggt ctggtaccgc ggcaacgaga cctatgtggg
     3421 cgagaccgag ccggaaggcg agggctgggt ccaggaagag gacgtgctcg acacctggtt
     3481 ctcctcggcg atctggccct tcgcgactct cggctggccg gacgaaaccg acgagctgaa
     3541 ggccagatcg ccgag
//
LOCUS       DAOQMF010000090         3534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13898_length_3534_cov_3.881288, whole genome shotgun sequence.
ACCESSION   DAOQMF010000090 DAOQMF010000000
VERSION     DAOQMF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13898_length_3534_cov_3.881288"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="PLV77_02325"
     CDS             complement(<1..1785)
                     /locus_tag="PLV77_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNC14715.1"
                     /translation="MEGLNEPETRRHPGKLFARILVVVLIDAAALLLIEAVLPGFDMH
                     GRFAALPTALGVGLVNALIWPVLSRFTLKLSVLTLGLWGLFLNAVLIGFALMAMPWVR
                     IEGPFEAIVISFGMAILTSLFSSLFAIDEDSTWYYNVVRTQLRRRGQVIETDEPGIVF
                     LEIDGLAHDILRRAMTNGNAPAMAAWVRDGSHKLEGWETDWSSQTGACQAGILHGNNH
                     DMPAFRWWEKETGRALVTNHPKDAAEMEARQSDGNGLLADDGASRANILSGDAPHSML
                     TMSTVMKRRGKIGQDYAAYFARPYGVIKTFIHTIAEIVRERRDNRRQERTGIEPKIER
                     SRVYAIMRAWATVIQLDLQISGVVGDILAGRPSIYTTFLAYDEVAHHSGIERPESLST
                     LAKVDREIDRIRRVVAEAPRPYHLVVLSDHGQSQGATFLQRYGVTLEEVVEKATGKEA
                     IGSGEEGEQNPRAYITASAEEASHEDGAIGSAATALARRDPNRDDLGLEGGPDEVAGG
                     EGKHEIPELSVMASGCLGLVTFPREPGRLTRERIDELYPGLLDTLVSHPGIGFVMVDS
                     EHGGVALGAEGLNYFEINDVEGVDPLAAF"
     gene            complement(1844..>3534)
                     /locus_tag="PLV77_02330"
     CDS             complement(1844..>3534)
                     /locus_tag="PLV77_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14716.1"
                     /translation="LGTGSFSGDLSYDNTTAGNTLDIIGSGQNPGQTVLNMVDTAGNN
                     EGFHFTSVPGSTIRNLTYRIPIAADGSSDVGLNLNGVTADGIVVDGPTASQVRAIACS
                     TCNLTGSTIDLPIENGTIGVDQTGGPAYVGDSVIKADYGVRHSSPGLTTTVERSRITA
                     HTGASTDGGNLEIFNTLILLDDQPNAVGINLYNPNEGTYAIGATLDHLTIISGGGQHS
                     IGIRAIADSESSGEGESVDATLTNSVIHGFNGTGEHTLDLLADKGRTVTFHSSYSAYD
                     NARTSNVTGSEGGTVDYELSPGHLDLEGGVSFVDPQSGDYTPSLLGGLLDGGDPIGPL
                     TGITDINGNPRACHGTDEGIIRQDIGAFEARTVVSSEPDDCTYPVTSILAKPPASTTA
                     KTASFTLGTDKNPGTFLCSLDGGPESDCGYTFETPTLSVGDHSLSVRAKDQFGNIDQS
                     PETYSWSVKDSGPGPCETNCVDKTAPKVIGVKAPKQTRAATVKVRFKSNEKGSTFKCR
                     LNKGKWKSCKSPWKTPRLRKGKNTVSIRAIDKAGNRSKVVTRVIRKVSKAKKRR"
BASE COUNT          549 a         1080 c         1214 g          691 t
ORIGIN      
        1 gaacgcggcc agggggtcga ccccttcgac gtcgttgatc tcgaagtagt tcaggccctc
       61 ggcgccgagc gcgacgccgc cgtgttcgga atcgaccatc acgaagccga tcccgggatg
      121 tgacaccagg gtgtcgagca ggccggggta gagctcatcg atccgttcgc gggtgagccg
      181 gcccggttcg cggggaaagg taaccagccc gaggcaaccg gaagccatca ccgacagttc
      241 cgggatctcg tgctttcctt cacctccggc gacttcatcc ggtccgcctt cgagcccgag
      301 gtcgtcgcgg ttcgggtccc gccttgccag cgcggtggcg gcgctgccga tcgcaccgtc
      361 ctcgtggctc gcctcctcgg cactggccgt gatgtaggcg cgggggttct gttcgccttc
      421 ctcgccggag ccgatcgcct ccttgccggt cgccttctcg accacttcct cgagcgtcac
      481 gccgtaacgc tgaaggaagg tagccccctg ggactggccg tggtccgaga ggacgaccag
      541 gtggtagggc cgcggggcct cggcgacgac ccggcggatc cggtcgatct cacggtcgac
      601 cttggccagg gtggagaggg attcgggacg ctcgatcccg gagtggtggg ctacctcgtc
      661 gtaggcgagg aaggtcgtgt aaatggaggg gcgaccggcg aggatgtccc cgaccacgcc
      721 cgagatctga aggtcgagct ggatcaccgt ggcccaggcc cgcatgatcg cgtagaccct
      781 cgagcgttcg atcttcggtt cgatcccggt tctttcctgg cgccggttgt cccgccgctc
      841 gcggacgatc tcggcgatcg tgtggatgaa ggtcttgatc accccgtagg gacgggcgaa
      901 ataggcggca tagtcctggc cgatcttgcc ccggcgtttc atcaccgtgc tcatggtcag
      961 catcgaatgc ggggcatcac cggagaggat gttggcccgg ctggcgccgt catcggccaa
     1021 caggccgttt ccgtccgact gccttgcctc catctcggcc gcatccttcg gatggttggt
     1081 cacgagggcc cggccggttt ccttctccca ccagcggaag gcgggcatgt cgtggttgtt
     1141 gccgtggagg atcccggcct ggcaggcgcc ggtctgggag gaccagtcgg tttcccagcc
     1201 ttccagcttg tggctgccat cccgcaccca ggccgccatc gccggggcgt tgccgttggt
     1261 catcgcgcgc cggagaatgt cgtgtgcgag gccgtcgatc tcgaggaaga cgatgccggg
     1321 ctcatcggtc tcgatcacct ggccgcggcg cctgagctgg gtgcggacca cgttgtagta
     1381 ccaggtgctg tcctcgtcga tcgcgaaaag cgaggagaag agcgaggtca ggatcgccat
     1441 cccgaaggag atcacgattg cttcgaacgg tccctcgatc cgcacccagg gcatcgccat
     1501 cagggcgaac ccgatcagca ccgcgttcag gaaaagtccc cagaggccga gggtcaggac
     1561 gctgagcttg agggtgaagc gggaaaggac cggccagatc aaggcgttga cgaggccgac
     1621 cccgagtgcg gtgggcaggg cggcaaaacg gccgtgcatg tcgaagcccg gcagaaccgc
     1681 ctcgatcagc aaaagcgcgg ccgcgtcgat caggacgacg accaggattc gggcgaacag
     1741 cttgccgggg tgccggcgcg tttccggctc gttcagtccc tccattgccg gaactctcgc
     1801 aaaccgggcg gtccccggca atcaccccga ccggatgaac ccctcagcgc ctctttttcg
     1861 ccttcgaaac cttcctgatc acccgcgtca cgaccttcga ccggttgccg gccttgtcaa
     1921 tcgcccggat cgacaccgtg ttctttccct tgcggagcct gggggtcttc cagggtgact
     1981 tgcagctctt ccacttgccc ttgttcaggc ggcacttgaa ggtcgatccc ttctcgttcg
     2041 acttgaagcg gaccttgacc gtggcggccc tggtctgctt cggggccttg accccgatca
     2101 ccttcggggc ggtcttgtcg acgcagttgg tctcgcaagg tcccggaccg gagtccttga
     2161 ccgaccagga gtaggtctcg ggcgactggt cgatgtttcc gaactggtcc tttgcgcgaa
     2221 cggacaggct gtgatcgccg acgctgaggg tcggggtctc gaaggtgtag ccgcagtcgc
     2281 tttccgggcc gccgtccagt gagcagagga aggtgcccgg gttcttgtcg gtgcccaggg
     2341 tgaaggaggc cgtcttcgcg gtggtgcttg cgggaggctt ggcaaggatc gaagtcaccg
     2401 ggtaggtgca gtcgtcgggc tcggagctga caaccgtccg ggcctcgaag gcgccaatgt
     2461 cctgacggat gatgccttcg tcggtcccgt ggcaggcacg agggttgccg ttgatgtcgg
     2521 tgatgccggt aagggggccg atcgggtcgc cgccatcgag gaggccgccg agcagggagg
     2581 gagtgtaatc gccactctgc ggatcgacga aggaaacgcc gccttcgagg tcgaggtggc
     2641 ccggcgaaag ctcgtagtcg accgtgccgc cctccgatcc ggtcacgttc gatgtcctgg
     2701 cgttgtcgta ggccgagtaa ctcgaatgga aagtgaccgt ccgtccttta tccgccaaaa
     2761 gatcaagcgt gtgctcgccg gttccgttga agccgtggat caccgagttg gtcagggtgg
     2821 catccacact ttcgccttcg ccgctcgact cgctgtcggc gatcgcgcgg atgccgatgc
     2881 tgtgctggcc gcccccgctg atgatcgtca ggtggtcgag ggtggcgccg atcgcatagg
     2941 tcccttcgtt ggggttgtag aggttgatcc cgaccgcgtt cggctgatcg tcgagaagga
     3001 tcagggtatt gaagatctcg aggttgccac cgtcggttga agccccggtg tgcgccgtga
     3061 ttcggcttct ttcgacggtt gtggttagac ccggggaact gtgcctgacc ccatagtcgg
     3121 ctttgatcac ggagtcgccg acatacgccg gtcctccggt ctggtcgacg ccgatagtgc
     3181 cgttctcgat cgggaggtcg atggtcgagc cggtcaggtt gcaggtgctg catgcgatcg
     3241 cccggacctg ggaggcggtc gggccatcga cgacgattcc gtcggcggtc acgccgttga
     3301 ggttgagacc gacatccgag ctgccatcgg cagcgatggg aattctgtaa gtcaggttcc
     3361 tgatcgtcga gccggggacc gaagtgaagt ggaagccttc gttgttgccg gccgtgtcaa
     3421 ccatgttcag gacggtctgg ccggggttct gcccggagcc gatgatgtcg agcgtgttgc
     3481 cggcagtggt gttgtcgtag gagaggtctc cggaaaagct gccggtgccc agtc
//
LOCUS       DAOQMF010000091         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_13988_length_3518_cov_3.636154, whole genome shotgun sequence.
ACCESSION   DAOQMF010000091 DAOQMF010000000
VERSION     DAOQMF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13988_length_3518_cov_3.636154"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLV77_02335"
     CDS             <1..354
                     /locus_tag="PLV77_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14717.1"
                     /translation="TDVIHGWNVPELTGKAQAVPGKTNEVYFRADDPGTYTSRSSVFS
                     GQGYDTMEIEVQVVTTDEYEAATIALKKDIQDAQDKVEAEFKASKAKAVVATAEGKKM
                     KAEINKDDIESADSQ"
     gene            351..2054
                     /locus_tag="PLV77_02340"
     CDS             351..2054
                     /locus_tag="PLV77_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC14718.1"
                     /translation="MSRTTSYPQTMPPTRPEISSDGPGSRPSRWAELASSADHKDIGR
                     LLIATSFGSIFLAALELLLMRVQLAVPENVFLKGVTFDRLLSVYGETAIFLVALPLII
                     GLLYYVVPLQIGSRTTAIPRVGQIGLWLFIFGGLLLYGSFLFTPPEAGINPLPPFSDA
                     TFTPNNGVDVWIGSTGLITLGLLMIAIDLLATLHNLRAPGMAWRRAPLFTWAGAIVTW
                     LFVVIAPIFLAAISMLLIDRLYDGGFFAGDAGGSPILWQHLSYIFFTGVYMAVSITFL
                     AAIGEIIQTFTGRELPRKAVVWSFVVIAIVGTLAWTQNMLTAPIGSGWKYYGMLMSLA
                     LIVPFGLIFYNLISTLGSSDFHMRAPLRFALGALSLASIGLAAEIAQSTIAVATQLSE
                     TYDAWAATHFTLIGFVLFGGFAAVTYWYPKMTGHDLNEDRAAKSFGLMFVGTLIALLP
                     LFAAGVSGQVTDSYRYFSYEDLGVYNAISAVGTLLLFVGVVLAVGNLVKSRSEEPAAT
                     PDPYRGDSLEWLALSPPPAHNFDVLPDVRSTRPMRDIREAVIRRDVEDAEAESAAQPV
                     A"
     gene            2060..3004
                     /locus_tag="PLV77_02345"
     CDS             2060..3004
                     /locus_tag="PLV77_02345"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNC14719.1"
                     /translation="MNSGADAHSVRTRQAAQPAQSGLQLDLIPMVRDYISLTKPRIIS
                     LLLLTTVATMFVADPSGPALSTILWTMLGGYLAAGGAGAINHYIDRNRDARNNRTKDR
                     PLADGRIKPINGLIFGIVLGVISFFQLWLTVNLLAAGLAMVGLFGYVFLYTLWLKPLT
                     PQNIVIGGSAGAVPPLVGWAAASGSLTWEAMWPFLIIFFWTPPHFWALSLMIKDDYAK
                     TGVPMLPVVKGEEATRQSILIYTIFMVGFTVGPYCTGLVGPFYLAASVVLGLIYIGFS
                     FMLWRRPSPKITLQSYLYSLAYLALLFISMAVAAVASA"
     gene            3213..3497
                     /locus_tag="PLV77_02350"
     CDS             3213..3497
                     /locus_tag="PLV77_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14720.1"
                     /translation="MSQITKPTETIQLPAPSFAPAVFALGVLGLIAGTFANDFMFPAW
                     FYAFVGFFFCLFSLRSTIKKGRRAFYALPREQGDARAELPVESFTAPTAD"
BASE COUNT          550 a         1289 c          991 g          688 t
ORIGIN      
        1 accgacgtga tccacggctg gaacgttccc gagctgaccg ggaaggccca ggccgttccg
       61 ggcaagacca acgaggtcta cttccgcgcc gatgacccgg gcacctacac gagccgctcc
      121 tcggttttct ccgggcaggg ctacgacacg atggaaatcg aagtccaggt cgtgaccacc
      181 gatgaatacg aggccgcgac catcgccctg aagaaggaca tccaggacgc ccaggacaag
      241 gtcgaagccg aattcaaggc cagcaaggcg aaggcggtcg tggccaccgc cgagggcaag
      301 aagatgaagg ccgaaatcaa caaagacgac atcgaaagcg ctgactccca atgagccgca
      361 caaccagcta cccccagacg atgccgccga cccggcccga gatctcctct gacggtcccg
      421 gctcacgccc ttcgcgctgg gccgaactcg cttccagcgc cgatcacaag gacattggcc
      481 ggctgctgat cgcaacctcc ttcggttcga tcttcctcgc cgcgctcgaa ctgcttttga
      541 tgcgggttca gctggcggtc ccggagaacg tcttcctcaa gggagtcacc ttcgaccgtc
      601 tgctctccgt ttacggcgag accgcgatct tcctggtcgc cctgccgctg atcatcggcc
      661 tgctctacta cgtggttccg cttcagatcg gcagccgcac caccgcgatc ccgcgggtcg
      721 gccagatcgg tctttggctg ttcatcttcg gtggcctcct gctttacggc tcgttcctct
      781 tcaccccgcc ggaagccggc atcaacccgc ttcccccctt ctccgacgcc actttcaccc
      841 ccaacaacgg cgttgacgtc tggatcggct cgaccgggct gatcaccctc ggcctgctga
      901 tgatcgcgat cgacctgctc gccaccctgc acaacctgcg cgcccccggc atggcctggc
      961 gccgggcgcc gctcttcacc tgggccggcg cgatcgtgac ctggcttttc gtggtgatcg
     1021 ccccgatctt cctcgcggcc atcagcatgc tgctgatcga ccgcctctat gacggtggct
     1081 tcttcgccgg tgacgccggc ggctcgccga tcctctggca gcacctcagc tacatcttct
     1141 tcaccggcgt ctacatggcc gtctcgatca ccttcctcgc ggcgatcggg gagatcatcc
     1201 agaccttcac cggccgggag ctgcctcgca aagccgtcgt ctggtccttc gtggtcatcg
     1261 cgatcgtcgg caccctggcc tggacccaga acatgctgac cgcaccgatt ggcagcggct
     1321 ggaagtacta cgggatgttg atgtcgctgg ccctgatcgt tcccttcggc ctgatcttct
     1381 acaacctgat ctcgaccctc ggctcgagcg acttccacat gcgggcaccg ctgcgcttcg
     1441 cgctcggcgc gctttcgctg gcttcgatcg ggcttgccgc cgagatcgcc cagtcgacca
     1501 tcgccgtcgc gacccagctt tcggagacct acgacgcctg ggccgccacg cacttcacgc
     1561 tgatcgggtt cgtgctcttc ggcggcttcg ccgcggtcac ctactggtac ccgaagatga
     1621 ccggccacga tctgaacgag gaccgtgccg ccaagtcctt cggcctcatg ttcgtcggca
     1681 ccctgatcgc attgctgccg ctgttcgcgg ccggggtctc gggccaggtc accgactcgt
     1741 accgctactt cagctacgag gatctcgggg tctacaacgc gatctccgcc gtcggcacgc
     1801 tgctcctctt cgttggtgtc gtcctcgcgg tcggcaacct ggtcaagagc cgcagcgaag
     1861 agcctgccgc cacgcctgac ccgtaccggg gcgattcact cgagtggctg gcgctctcgc
     1921 cgcccccggc ccacaacttc gacgtcctgc cggacgttcg cagcacccgc ccgatgcgtg
     1981 acatccgcga ggcggtaatc cgccgcgatg tcgaggacgc agaggccgag agtgccgccc
     2041 agccggtagc ctgaatgtca tgaactccgg tgccgacgcg cactcagtgc gtactcggca
     2101 ggccgcgcag cccgcgcagt ccggcctgca gcttgatctg atcccgatgg ttcgggatta
     2161 catctcgctg accaaaccgc ggatcatcag cctgctgctg ttgaccacgg tggcgacgat
     2221 gtttgtggcc gacccctccg gccccgccct ctccaccatc ctctggacca tgctcggcgg
     2281 ttatctggcg gccggtggcg ccggagcgat caaccactac atcgaccgca accgcgacgc
     2341 gcgcaacaac cggaccaagg accggcccct ggccgacggt cggatcaagc cgatcaacgg
     2401 gctgatcttc ggcatcgtcc tcggggtcat ttccttcttc cagctctggc tcacggtcaa
     2461 cctgctcgca gccggcctgg cgatggtcgg tctgttcggg tacgtctttc tctacacgct
     2521 ctggctgaag ccgctgaccc cgcagaacat cgtgatcggt ggcagcgccg gggccgttcc
     2581 gccgctggtc ggctgggccg ccgcctcggg ttccctgacc tgggaggcga tgtggccctt
     2641 cctgatcatc ttcttctgga ccccgcccca tttctgggca ctctcgctga tgatcaagga
     2701 cgattatgcg aagaccggcg tgccgatgct gccggtggtc aagggcgagg aagcgacccg
     2761 ccagtcgatc ctgatctaca cgatcttcat ggtcggattc acggtcggtc cctactgcac
     2821 ggggctggtt ggccccttct acctggccgc ctcggtggtt ctcggcctga tctacatcgg
     2881 cttctcgttc atgctctggc gccggccgag cccgaagatc acgctccagt cctacctcta
     2941 ctcgctggcc tacctcgccc tgcttttcat cagcatggcc gtggcagccg tggcttccgc
     3001 gtaacctgac cgcaggcgcg ccggtcttcc ccggcgggac tccgcttcag gcgaccggaa
     3061 cgccccccga attgatagaa ataggtccca tggatcgcaa cacttccaag aaaaacttca
     3121 ccacgggcat gctgctcggc gcgacttccg cgtccatctt cgccctcact ttcgtctttg
     3181 cggtcctcta catcgcgaac tagggacaac tcatgagcca gatcaccaag ccgaccgaaa
     3241 cgatccagct tcccgctccc tccttcgccc cggcagtctt cgccctcggc gtgctcggcc
     3301 tgatcgccgg caccttcgcc aacgacttca tgttcccggc ctggttctac gccttcgtcg
     3361 gcttcttctt ctgcctcttc tcgctgcgca gcacgatcaa gaagggccgc cgcgccttct
     3421 acgccctgcc ccgcgagcag ggtgacgccc gggccgagct cccggtcgag agcttcaccg
     3481 cccccacggc tgactgaccc ctccgggcaa tactcgcc
//
LOCUS       DAOQMF010000092         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_14032_length_3513_cov_3.596588, whole genome shotgun sequence.
ACCESSION   DAOQMF010000092 DAOQMF010000000
VERSION     DAOQMF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14032_length_3513_cov_3.596588"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLV77_02355"
     CDS             <1..834
                     /locus_tag="PLV77_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNC14721.1"
                     /translation="GTGGKTASESPAPTGPVKLKVADYSVNFGGRFSLRGKTGAARKG
                     HVRIQSRVNGRWHNLRKAGTDGMGRYRLKLRARHNLVIRAVAGDGRVSKTRRVTVIGH
                     IRLDRSERYVKLGRKLRVSGVVVPRGVRTVKVRVRGEGVMRTRSRANGRFAFRWTPRR
                     NGDFSYRVWARKSRKSTGDSSRPHRFSALRPGEASYYGPGLYGNGVACGGTLTPTTRG
                     VANKYLPCGTRVTLQYGNRTVVVRVIDRGPYGAGRDWDLTEQTKKDLGFGGVGTVWTN
                     K"
     gene            950..1297
                     /locus_tag="PLV77_02360"
     CDS             950..1297
                     /locus_tag="PLV77_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14722.1"
                     /translation="MSSAESKNLEAISKAIDHHNATCPFPAAEVRMNPFEVERLGWEE
                     IRGLPIVPDPNIGTGRFHIVCSRDMEGDELEEVEAIAEELVTVTPGKRDAPAEPGQEP
                     GEPGIPDNPGRWN"
     gene            1382..2935
                     /locus_tag="PLV77_02365"
     CDS             1382..2935
                     /locus_tag="PLV77_02365"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNC14723.1"
                     /translation="MLLSAAIWTSSAEAGRIAYVADYDANVVSQLDTETNAFLSTSFS
                     GMTDTYMLQVSPDGKYLWIPRFTPNKVSVVDLTDGQALPDIDVDQNPVGVVFSPDSSM
                     AYVLSYDASTVQVFDTATRQQIGDDIPVGAAPYYESLSPDGSRLYVANFDDDTISVIN
                     TADRQVVGDPVPVCADLYSMAISPDGTRLFIGCAGSGDMVMQLSTDTMEATSFSATLP
                     EDPEYLTISPDGATGWAVIRTLDEVVSFSTATGAAIGDPIPVGHEPYHLAITPDGSKA
                     FTSNYEGTQGITPINIATRVAGTLIPDTGGPWQMAITPDQPPSALIAAPISIVAGQPA
                     KFDAVNSIDPDGTISRYDWNFGDGKSKPDGKVTESHVFTKAGQFSVSLILTDNEGCST
                     GFVTAGYTAYCNGGAGASTTLNVRVKAPSNNFSFGRIKLNRKRGTASLQVRLPGPGVV
                     MLAGKNVRGARKTVKKAGKSALSVRITGKAKRALSGRGVVKVKAKVTYRPKYGKPRTK
                     TRTVTLRRN"
     gene            complement(2943..>3513)
                     /locus_tag="PLV77_02370"
     CDS             complement(2943..>3513)
                     /locus_tag="PLV77_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNC14724.1"
                     /translation="IFVGSFSLIFILLGMGATRIGSLLSDNQELLEKISGALIIALGV
                     LFVASFFVTRLNREWHVDALMQRAGKGGPLVAGAAFAFAWTPCIGPTLGAILSAASTT
                     DSTAKGALLLAVYSAGLAIPFLLTALAFSKMTSAFAVIKRHYGVVIMVGGLVLIAMGI
                     LIWTGELSRINIEIQQWSQETGLDFWNSV"
BASE COUNT          680 a         1129 c         1163 g          541 t
ORIGIN      
        1 ggcaccggcg gcaagacggc cagcgagtcg cccgccccga ccggcccggt caaactgaaa
       61 gtggctgact acagcgtcaa cttcggcggc aggttcagcc tgcgcggcaa gacgggcgcc
      121 gcccgcaagg gtcacgtccg gatccagtcg cgggtcaacg gacgctggca caacctgcgc
      181 aaggccggaa ccgacggcat gggccgctac cggttgaagc tgcgtgcccg ccacaacctg
      241 gtgatccgtg ccgtggcggg tgatggccgg gtttcgaaga cccgtcgcgt cacggtcatc
      301 ggccacatca ggctcgaccg ctccgagcgc tacgtgaagc tcggccggaa gctccgggtc
      361 agcggcgtgg tcgtgccacg cggagttcgc acggtcaagg ttcgcgttcg cggtgaaggt
      421 gtgatgagga cgaggagccg ggccaacggt cgtttcgcct tccgctggac tccgcgccga
      481 aacggcgact tcagctaccg ggtctgggcg agaaagagcc gcaagtcaac cggtgactcc
      541 agccggccgc accggttcag tgccctgcgt ccgggcgaag cctcctatta cggtcctggg
      601 ctttacggca acggggtcgc ctgtggcggc acgctcaccc cgaccacgcg tggcgtcgcc
      661 aacaagtacc tgccctgcgg caccagggtc acgcttcagt acggcaaccg caccgtggtc
      721 gtccgggtaa tcgaccgggg gccgtacggt gccggccggg actgggacct gaccgagcag
      781 accaagaagg acctcgggtt cggcggcgta ggcaccgtct ggacgaataa atagtcggcg
      841 ccggcttcgc cgggcctccc ggtgaaaccc gtccgttcac ggaatcaatg aatggatgaa
      901 cggactccgg cttcggttgg gccgggctca cttcggcata atccggagca tgagctcggc
      961 cgaatcgaag aacctcgagg cgatctcgaa ggcgattgac catcacaacg ccacctgccc
     1021 ctttccggcg gccgaggtgc ggatgaatcc cttcgaggtc gagcggctgg gctgggagga
     1081 gatccgcggc ctgccaatcg tccccgaccc caacatcggc accggacgct tccacatcgt
     1141 ctgctcccgc gacatggaag gcgacgaact cgaagaggtc gaggcgatcg cggaggagct
     1201 ggtgacggtc acgcccggga agagggatgc gccggccgaa ccgggccagg agccggggga
     1261 acccgggatc ccggacaacc cggggcgctg gaactgaaca gctccccttg atccccggcg
     1321 gagcgcggac tagtctgctc tcatgagatc ccgggggagg gtatgggttt tgctggccgg
     1381 aatgctgctc tcggcagcga tctggacctc gtcggctgaa gcggggcgga tcgcctacgt
     1441 cgccgattac gacgcgaacg tcgtgtcgca gctggacacg gaaacgaacg ccttcctgtc
     1501 cacttccttt agcggaatga ccgataccta catgctccag gtgtctcccg acgggaagta
     1561 cctgtggatc ccgaggttca ccccgaacaa ggtttcggtg gtcgacctga ccgacggcca
     1621 ggcgctgccg gacatcgatg tcgatcagaa cccggtcggg gtggtctttt cccccgacag
     1681 ttcgatggcc tacgtgctca gttacgacgc cagcacggtc caggtcttcg acacggccac
     1741 ccgccagcag atcggcgatg acattccggt cggggccgcc ccctactacg agtcactctc
     1801 tccggacgga agccgtctct acgtcgcgaa cttcgacgac gacacgatct cggtcatcaa
     1861 caccgccgac cgccaggtgg tcggcgatcc ggtcccggtc tgcgcggacc tgtactcgat
     1921 ggccatttca ccggacggga cccggctctt catcggctgt gccggtagcg gagacatggt
     1981 gatgcagctt tcgaccgaca ccatggaagc gacgagcttc tcggcgaccc tgcccgagga
     2041 tcccgagtac ctgaccatca gtcccgacgg ggccaccggc tgggcggtga tcaggacgct
     2101 cgacgaggtc gtttccttca gtaccgcgac cggggccgcg atcggcgatc cgatcccggt
     2161 cggccatgag ccgtatcacc tggcgatcac cccggacggg tcgaaggcat tcacttcgaa
     2221 ctacgagggc acccagggca tcactccgat caacatcgcg acccgggttg ccggaacgct
     2281 gatccccgac accggcggtc cctggcagat ggcgatcacc cccgatcagc cgccgtcggc
     2341 gttgatcgcg gcgccgatca gcatcgtggc cggacagccg gcgaagttcg atgccgtcaa
     2401 ctcgatcgac ccggacggca cgatcagccg gtacgactgg aacttcggtg acggcaagag
     2461 caagcccgac ggcaaggtga ccgaaagtca cgtattcacg aaggcgggac agttttccgt
     2521 gtcgctgatc ctgaccgaca acgagggctg ctccaccggg ttcgtgaccg ccgggtacac
     2581 cgcttactgc aacggcgggg ccggggcatc gacgacgctc aatgtcaggg tgaaagcgcc
     2641 atccaacaac ttcagcttcg gcaggatcaa actgaaccgg aagcggggaa cggcatccct
     2701 tcaggtcagg ctccccggcc ccggtgtcgt gatgctggcc ggaaagaacg ttcgtggtgc
     2761 ccgcaagacg gtgaagaagg caggcaagtc tgccctgagc gttcggatca cgggcaaggc
     2821 gaagcgggcg ctaagcggca ggggcgtcgt caaggtcaag gccaaggtga cctacaggcc
     2881 gaagtacggc aagccgcgca ccaaaaccag aacggtgacc ctgcgccgga actgatccgg
     2941 cctcagaccg agttccagaa atcgagaccg gtttcctggg accactgctg gatctcgatg
     3001 ttgatgcggg agagctcgcc ggtccagatc aggattccca tcgcgatcag gaccagaccg
     3061 ccgaccatga tcacgacccc gtaatgccgt ttgatcacag cgaaggcgct ggtcatcttc
     3121 gagaaggcaa gggcggtgag caggaagggg atcgccaggc cggccgaata gacggccagc
     3181 agcagggcgc ccttggcggt tgagtcggtg gtcgaggcgg cggagagaat cgcgcccagg
     3241 gtgggtccga tgcacggggt ccaggcgaag gcgaaggcgg caccggcaac cagcggcccg
     3301 ccctttccgg cccgctgcat cagtgcgtcg acatgccact ccctgttcag gcgggtaacg
     3361 aagaaggacg cgacgaagag gacgccgagc gcgatgatca gcgccccgga aatcttctcc
     3421 agaagctcct ggttgtcgct gaggagcgaa ccgatcctgg tcgcacccat cccgagcagg
     3481 atgaagatca gcgagaagct gccaacgaag atg
//
LOCUS       DAOQMF010000093         3502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_14120_length_3502_cov_3.664346, whole genome shotgun sequence.
ACCESSION   DAOQMF010000093 DAOQMF010000000
VERSION     DAOQMF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14120_length_3502_cov_3.664346"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..831
                     /locus_tag="PLV77_02375"
     CDS             226..831
                     /locus_tag="PLV77_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNC14725.1"
                     /translation="MPATENEIKEKYLERAIRELNQLNRDLQGCDACLTTKVMPVLGS
                     GHPQADVMLVKFAPTTAEVEEGVAFYGRAGNALMKSFKRLNIDPTVIYGTVCAKCPVE
                     DPTRADPECVARLADEMGIVQPRIVVVMGEPALETINSLALPLARPLEPREGEIQAFS
                     PACDALFTPDIDGALDEETAKRRFWRAFRTLGRWHDDLPPY"
     gene            983..1378
                     /locus_tag="PLV77_02380"
     CDS             983..1378
                     /locus_tag="PLV77_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14726.1"
                     /translation="MGTVEQTVIGPREGHANGKALAAMILGVFAIANVVVLALFMALS
                     AETLDDSGAWWMLLPFFGLTAGLIAAFLGSLGWIDVRRGITDRRLFEAQLGAILGGVA
                     AGLILLAITVLLVMALFLAFSFAGEGGVD"
     gene            complement(1399..2124)
                     /locus_tag="PLV77_02385"
     CDS             complement(1399..2124)
                     /locus_tag="PLV77_02385"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNC14727.1"
                     /translation="MPDREKISRYAKATAKAGKGGELADLLLEAAEVNRASEGCLQYE
                     VHQSVFDPDTIWVTEAWVDQDAVKASLEDEENKTLIDRARPLIEDMEVIELLPLGGIE
                     ARLDDPRAATPKPGYKLVNVAEVNDGAKDHGLGEIQEARFAIGDLEASQVGVAWFRMH
                     PNQRQPFGHVHDRAEEVYFVVSGSGRVKLDDDIVELREGDLIRVAPEIIRCFEAGPDG
                     MVYYAAGQHMSGDGDVKPGWWSD"
     gene            complement(2169..2774)
                     /locus_tag="PLV77_02390"
     CDS             complement(2169..2774)
                     /locus_tag="PLV77_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNC14728.1"
                     /translation="MSAPPLDPAPSPSPPSDERPGMDVTRPRTPAGMIGWSIFASRWI
                     QAPLYLGLIVAQAVYVVKFWKELVHLITDFSSFDENAVMLTVLGLVDVVMIANLLIMV
                     IIGGYETFVSRIKVENHPDQPEWLSHVNANVLKVKLAMAIVGISSIHLLKTFIEYGTE
                     ETGLTSTEVMWQTIIHFTFVLSAIGLAYIDRYLSPTKDDGL"
     gene            complement(2839..>3502)
                     /locus_tag="PLV77_02395"
     CDS             complement(2839..>3502)
                     /locus_tag="PLV77_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC14729.1"
                     /translation="KGLETLAEFAKGADGVELGVAVIALDRQSPAEIAADVERLGLDP
                     SRLWIGVGAGFTKKPLTFMTEQIGELREKLPGIRVIMAAMGPKMCALAGSEYDGAFFN
                     WMTPEFAAGARTKVEAGAADAGHEAPPVFGYVRTAVGPDAAERLAKEESFYRDLHDGY
                     RNHFARLGEPEGTVGVAAADSAEAQEKLAAYEALDVIVVRGLASAKVEPMVELARAAA
                     PA"
BASE COUNT          580 a         1191 c         1134 g          597 t
ORIGIN      
        1 cgtttacgtt caggcgctga agttcattca gaccaccggg cggcccaagg cggccaagcg
       61 tcaggtcgat gacccgcagg ccgtcagagg cggccggcgc aaggcccgtg gctcgaagca
      121 ggcgcgtggg ccaaaacgtg cagcaggcaa gagctgatgc acggacccgg tgacagccgg
      181 gatccggcag gagccccgcc gggggcgtct aataaggcat cagcgatgcc tgcgaccgaa
      241 aacgagatca aagagaagta tctggaacgc gcgatccggg agctgaatca gctgaaccgg
      301 gatctgcagg gctgtgacgc ctgtctgacc acgaaggtga tgccggtgct cggctccggt
      361 cacccgcagg ctgacgtgat gctggtcaag ttcgccccga ccaccgccga ggtggaggag
      421 ggcgtggcgt tctacggccg ggccggcaac gccctgatga agtccttcaa acgactcaac
      481 atcgacccga ccgttatcta cggcacggtc tgcgccaagt gcccggtcga ggatccgacc
      541 cgggccgacc cggaatgcgt ggcccgcctg gccgacgaga tgggcatcgt ccagccccgc
      601 atcgtcgtcg tgatgggtga gcccgccctg gagacgatca acagtctcgc cctgccgctc
      661 gcccgtcccc tcgagccccg cgaaggcgag atccaggcct tcagccccgc ctgcgacgcc
      721 ctctttaccc cggacatcga cggtgccctc gacgaagaaa ccgccaagcg ccgcttctgg
      781 cgagccttcc gcaccctcgg ccgctggcac gacgacctcc ccccgtacta acccgcccgg
      841 caccagcacc tgccggaaac cgccccgcct tcaccgcccc gccggcccgc tccggccccg
      901 cgagattcca ctttgcgcca cataccggca caaaccgggc actcggtgac ttctttaggg
      961 gatgtcggac taaaattccg gcatgggaac cgtcgaacaa acggtgatcg gtccccgcga
     1021 gggccatgcg aacggcaagg cgctcgcggc gatgatcctc ggcgtcttcg cgatcgccaa
     1081 cgtggtcgtc ctggccctct tcatggccct gagcgccgag acgcttgacg acagcggcgc
     1141 ctggtggatg ctgctcccgt tcttcggcct caccgccggc ctgatcgcgg cttttctcgg
     1201 atcgctcggc tggatcgacg tccgccgtgg catcaccgac cgccgactct tcgaagccca
     1261 gctcggcgcg atcctcggtg gcgtcgcggc cggactgatc ctgctcgcga tcaccgttct
     1321 cctggtgatg gccctcttcc tcgccttcag cttcgccggc gagggcgggg ttgactgatc
     1381 accccggccg gggcaggttc agtcgctcca ccagccgggc ttgacgtcgc cgtcgcctga
     1441 catgtgctgg ccggcggcgt agtagaccat cccgtcgggt ccggcttcga agcagcggat
     1501 gatttccgga gcgacccgga tcaggtcgcc ttcccggagc tcgacgatgt catcgtccag
     1561 cttgacccgg cccgagccgg aaaccacgaa gtagacctcc tcggcccggt cgtggacgtg
     1621 gccgaagggc tggcgctggt tcggatgcat gcggaaccag gccacgccca cctggcttgc
     1681 ttccaggtca ccgatcgcga agcgggcctc ctggatctcg ccgagaccgt ggtcttttgc
     1741 cccgtcgttt acttccgcga cgttgaccag cttgtatccc ggcttcgggg tggcggcacg
     1801 ggggtcgtcc agcctggcct cgattccgcc gagcggcagc agctcgatca cttccatgtc
     1861 ctcgatcagg gggcgcgccc ggtcgatcag ggtcttgttt tcctcatcct ccagcgaggc
     1921 cttcaccgca tcctggtcaa cccaggcctc ggtcacccag atcgtgtccg ggtcgaagac
     1981 ggactggtgc acctcgtact ggaggcagcc ttcggaagcg cggttgacct cggctgcctc
     2041 aagcagcagg tcggcgagtt cacctccctt gccggctttc gcggttgcct tggcgtagcg
     2101 ggagatcttc tctcggtcgg gcatgtccgg actctacccg cgaatcgcgg cgaccaagcc
     2161 gcgaccgctc agagcccgtc gtccttggtc ggcgagaggt aacggtcgat gtaggcgaga
     2221 ccgatcgccg aaaggacgaa ggtgaaatgg atgatggtct gccacatcac ctcggtggag
     2281 gtcaggcccg tctcttcggt tccgtactcg atgaaggtct tgagcaggtg aatcgaggag
     2341 atgccgacga ttgccatcgc cagcttgacc ttgagcacgt tggcattcac atgcgaaagc
     2401 cactccggct ggtcggggtg gttctcgacc ttgatccggg aaacgaaagt ctcgtagccg
     2461 ccgatgatca ccatgatcag caggttggcg atcatcacca cgtcaaccag accgagaacc
     2521 gtcagcatga cggcgttttc gtcgaagctg ctgaagtcgg tgatcaggtg gaccagttcc
     2581 ttccagaact tgaccacgta gaccgcctgg gcgacgatca ggccgaggta gagcggggcc
     2641 tggatccagc ggctggcgaa aatcgaccat ccgatcatcc ccgccggagt gcgggggcgg
     2701 gtcacgtcca tgccgggacg ttcatcggag ggaggggagg gactgggcgc agggtcgagc
     2761 ggaggtgcgg acatcgacgc aaatccaacc agagcgaccg gcggaaaacc gctctttatc
     2821 gggatttgcg ggggtttatc aggcgggtgc cgcggccctg gcgagctcga ccatcggctc
     2881 gaccttcgcc gaggcgagac cgcgcaccac gatcacgtcg agcgcctcgt aggcggccag
     2941 cttctcctgg gcttcggccg aatcggcggc cgccaccccg accgttccct ccggctcgcc
     3001 gaggcgggcg aagtggttcc ggtagccgtc gtgaaggtcg cggtaaaagg actcttcctt
     3061 tgccaggcgc tccgccgcat cggggccgac cgcggtgcgg acataaccga agacgggcgg
     3121 ggcctcgtgc ccggcatcgg cggcgccggc ctcgaccttg gtccgggcgc cggcggcgaa
     3181 ctccggggtc atccagttga agaaggcgcc gtcgtactcg gagccggcca gggcacacat
     3241 cttcgggccc atcgcggcca tgatcacccg gatcccgggc agcttctccc ggagctctcc
     3301 gatctgctcg gtcatgaaag tcagcggctt cttcgtgaat ccggctccga ccccgatcca
     3361 gaggcgggag gggtccagcc ccagccgttc gacgtcggcc gcgatctcgg ccgggctctg
     3421 ccggtcgagg gcgatcacgg cgaccccgag ctcgaccccg tcggcaccct tggcgaactc
     3481 ggccagcgtt tcaagcccct tg
//
LOCUS       DAOQMF010000094         3481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_14264_length_3481_cov_3.605663, whole genome shotgun sequence.
ACCESSION   DAOQMF010000094 DAOQMF010000000
VERSION     DAOQMF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14264_length_3481_cov_3.605663"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLV77_02400"
     CDS             <1..367
                     /locus_tag="PLV77_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC14730.1"
                     /translation="LGRVRAQLRRRELDRAPAPEQTRISAGPVTIDLARHVVTVNGKT
                     VSLTRSEFQLLRLLAARPGEVFSRAKIMEELWQTEFDGDERACDVHISNLRQKVETDP
                     QRPELVLTVRGIGYKFADS"
     gene            495..1730
                     /locus_tag="PLV77_02405"
     CDS             495..1730
                     /locus_tag="PLV77_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC14731.1"
                     /translation="MATLVTELDLPGIDPADPELKGDRWHVAANGLLDSGKWLAQGPL
                     SLVVIDREAGEFFLRSRSAEFPGRLIADLFGIKDGPLRDQVDANIINRSGDDHRRLRS
                     LVNPALTPRAANRWRPVMREILEGLWEELETKEFDFVSRFSRPYPSQTIARVMGAPAV
                     DASRLHDWSEWIQRQFDPIALSDPETVETVQDKVAEFYAWVDPLIEERRGTPADDLIT
                     TLIRTEEEGEKLSDQELRNLVLNILVGGVDTTQSQLSQAIRLLAAAPDQWGLLREDPE
                     KMVPQAVSEVLRFEPVTAFTARQLVEEVEYRDVTFPAGTLLVVCSFTGNRDPEAFGDP
                     RDFDIAAERGKTRPLTFGAGIHYCVGSNIARAELEEGLRFLAEKVETIELNGLTELQA
                     VSGIYGIDRMDVRIQPVSG"
     gene            1740..3140
                     /gene="wecB"
                     /locus_tag="PLV77_02410"
     CDS             1740..3140
                     /gene="wecB"
                     /locus_tag="PLV77_02410"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNC14732.1"
                     /translation="MTEQKGTDKPVPVKILVVVGTRPEAIKLVPMILALKENEMFIPQ
                     IVSTGQHQEMVKEVFDLAGIEIDFNLWVGGARNELNDRVREVMARLDDFVRVEYGADG
                     TVKQDEAAAGEYPMAIMVHGDTTSAFAAALAAFHLRIPVIHVEAGLRTQSNLTPFPEE
                     LNRQLITCIAAFNLAPTSHNLENLVRENVPVSQIFITGNTGIDSLQWAAGLEVEFSDP
                     IVAEICSSDRRVVTITAHRRENWGAGLDGIVAGVRLLAIEHPNTYFVVPQHPNPAVRQ
                     QLANLGDLPNVCLTDALSYPEFARLMARSYLIITDSGGIQEEAPSLGKPVLVCRESTE
                     RGEGVEAGTLRLVGTDPARIQAEADLLLKSEAAYQAMSEAENPYGDGRASERIVAALT
                     NLYVGGKMPEPFRSTYSRNAVIKASGYTLPGEASGRLDPALVPESERHHEEAVVDPVG
                     NLWAEFGTITEKWFRT"
     gene            3125..>3481
                     /locus_tag="PLV77_02415"
     CDS             3125..>3481
                     /locus_tag="PLV77_02415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC14733.1"
                     /translation="MVPHLIFAEWYPFLGLDPFFEVLFLITFLIVVAMFFWTVVLFFR
                     GIKSDRHLARQEEVNPELFEWIFLVPALNEEVTIADSVSRLEAIEVARKRIVVINDGS
                     DDRTAEILAERTDPDLY"
BASE COUNT          618 a         1163 c         1124 g          576 t
ORIGIN      
        1 gctcggccgg gtccgggccc agctccggcg ccgcgagctt gatcgcgccc ccgcccccga
       61 gcagacccgg atctccgccg gaccggtcac gatcgacctg gcccgccacg tggtgaccgt
      121 caacggcaag acggtcagcc tgacccgttc cgagttccag ctgctccgcc tgctcgccgc
      181 ccgtcccggt gaagtgttca gccgggccaa gatcatggaa gagctctggc agaccgaatt
      241 cgacggagac gaacgcgcct gcgacgttca catctccaac ctccgccaga aggtcgaaac
      301 cgacccccag cgccccgaac tggtcctcac agtccgcgga atcggctaca agttcgccga
      361 ttcctgaccg cagtcgagcc ttcggccttc ctgtaaccct ggcgccggtt ggcgtgacct
      421 gcttcgagca gcggccccgc cgacccggga catccataag cggatgggtc ggctacaggg
      481 gatagccttc gggaatggcg accctggtca ccgaactgga cctgcccggg attgatccgg
      541 ctgatcccga attgaagggg gaccggtggc atgttgcggc gaacggactg ctggactcgg
      601 gcaagtggct ggcgcagggg ccgctttcgc tggtggtcat tgaccgggag gcgggggagt
      661 tctttctgcg ttcccggtcg gccgaattcc ccggccggct catcgccgac ctgttcggga
      721 tcaaggacgg gccactcagg gaccaggttg acgccaacat catcaaccgg agcggtgacg
      781 accaccggcg gctgaggagc ctggtcaatc ctgccctgac cccgcgggcg gccaaccggt
      841 ggcgaccggt catgcgggag atcctcgaag ggctctggga ggaactggaa accaaggagt
      901 tcgacttcgt ctcgcgtttc agccggccct atccctccca gaccatcgcc cgggtgatgg
      961 gcgcaccggc ggtcgatgcc tcccgacttc acgactggtc cgaatggatc cagcgtcagt
     1021 tcgacccgat cgccctctcc gacccggaga ccgtcgagac ggttcaggac aaggtcgccg
     1081 agttctacgc ctgggtcgat ccgctgatcg aggagcgtcg cggcaccccg gccgatgacc
     1141 tgatcaccac cctgatcagg accgaggagg aaggggagaa gctctccgat caggaactcc
     1201 ggaacctggt cctgaacatt ctggtcggcg gggtcgacac cacccagagc cagctttccc
     1261 aggcgatccg gctgctggcc gcagcacccg atcagtgggg gctgctccgc gaggacccgg
     1321 aaaagatggt cccgcaggcg gtcagcgagg tactgcgttt cgaaccggtc acggccttca
     1381 ccgcccgtca gctggttgag gaagtcgagt accgggacgt gaccttcccg gccggcaccc
     1441 tgctggtggt ctgttccttc accgggaacc gggacccgga ggccttcggg gatccgcggg
     1501 actttgacat cgccgcggag cgcggcaaga cccggccgct caccttcggc gccggcattc
     1561 actactgcgt cggctcgaac atcgcccggg ccgaactcga ggagggcctg cggttcctcg
     1621 cggagaaggt cgaaacgatc gaactgaacg gcctgaccga gctgcaggcg gtcagcggga
     1681 tttatggcat agaccgcatg gatgtgcgga ttcagcctgt atccgggtag gcttcgcgaa
     1741 tgaccgagca gaagggaacc gacaagccgg ttccggtgaa gatcctcgtg gtcgtcggca
     1801 cccggccgga ggcgatcaag ctggttccga tgatcctcgc tctcaaggag aacgagatgt
     1861 tcatcccgca gatcgtttcc accggtcagc accaggaaat ggtcaaggaa gtcttcgatc
     1921 tggccggaat cgagatcgac ttcaacctct gggtcggcgg ggcgcgcaac gaactgaatg
     1981 accgggtccg ggaggtgatg gcccggctgg acgatttcgt ccgggtcgag tacggggccg
     2041 acggcacggt caaacaggac gaggccgcag ccggtgaata cccgatggcg atcatggtcc
     2101 acggcgacac gacctcggcc ttcgctgccg cgctggccgc cttccacctg aggatcccgg
     2161 tgatccacgt cgaagcgggc ctcaggaccc agtcgaacct gaccccgttc ccggaggaac
     2221 tgaaccggca gctgatcacc tgcatcgctg ccttcaacct cgcaccgacc tcccacaacc
     2281 tcgagaacct ggtccgggaa aacgtgccgg tcagccagat cttcatcacc ggcaacaccg
     2341 gaatcgactc gctgcagtgg gcggccgggc tcgaggtcga gttctccgat ccgatcgtgg
     2401 ccgaaatctg ctccagtgac cggcgcgtcg tcaccatcac ggcccaccgg cgcgagaact
     2461 ggggcgccgg gctcgacggt atcgtcgccg gtgtccggct gctcgcgatc gagcatccga
     2521 acacctactt cgtcgtcccg cagcatccga atccggcggt tcggcagcag ttggcgaacc
     2581 ttggcgacct gcccaacgtc tgcctgaccg acgcgctttc ctaccccgag ttcgcccggc
     2641 tgatggcccg ctcctatctg atcatcaccg actcgggcgg gattcaggag gaggcaccat
     2701 cgctcggcaa gcctgtgctg gtctgccgcg aatcgaccga acgcggggag ggcgtcgagg
     2761 ccggcacact tcgcctggtc gggaccgacc cggcaaggat ccaggccgaa gccgacctgc
     2821 tgctgaagag cgaggctgcc taccaggcga tgagcgaagc ggagaacccg tacggcgacg
     2881 gacgggcctc ggagcggatc gtcgcggcgc ttaccaatct ctacgtcggt ggaaagatgc
     2941 cggaaccttt ccgctccacc tacagccgca acgccgtgat caaggcgtcg ggctacaccc
     3001 tgccgggtga ggccagcggc cggctcgacc ccgcgctggt gccggagtcc gagcgtcatc
     3061 acgaggaggc ggtcgtggac ccggtcggca atctctgggc cgagttcgga acgatcacag
     3121 agaagtggtt ccgcacctga tctttgccga gtggtacccc ttcctggggc tggacccgtt
     3181 cttcgaggtc ctcttcctga tcaccttcct gatcgtggtc gcgatgttct tctggacggt
     3241 cgtcctgttc ttccggggga tcaagtccga tcgccacctg gcccggcagg aggaagtcaa
     3301 cccggagctg ttcgagtgga tcttcctggt cccggccctg aacgaggagg tgacgatcgc
     3361 cgacagcgtc agccgtctgg aagcgatcga ggtggcccgc aagcggatcg tcgtgatcaa
     3421 cgacggctcc gacgaccgca ccgcggagat cctcgccgaa cgcaccgacc cggacctcta
     3481 c
//
LOCUS       DAOQMF010000095         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_14365_length_3465_cov_3.654545, whole genome shotgun sequence.
ACCESSION   DAOQMF010000095 DAOQMF010000000
VERSION     DAOQMF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14365_length_3465_cov_3.654545"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLV77_02420"
     CDS             <1..915
                     /locus_tag="PLV77_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNC14734.1"
                     /translation="VDVVQRAMDGGAVGVVGVLADYFDSKYYFNEEYRKLQMDIPGMW
                     VTRSEGDAIRAQLSVSPDAEAKLVMNGEREAAPARNVIGFLPGQSRTTLLISSHHDAV
                     WDGAVEDGSGTSEVLALARYFGALKKKYRPMSLMFATMDSHFTGYQGHAAFLKKYVEN
                     APEAERPRVNFAIEHIARHGYVDENGKLKMTNLPEIAGILENVGEKPLNVIRKVIPKY
                     GLDRTVVLPATFFGVSLPTDASGAYQEAVPVVSLISGPIYLYDRQDTLDKVYKPHLRP
                     MALAYKEMIARMSRMDPEDLPGDPPYKP"
     gene            893..2161
                     /locus_tag="PLV77_02425"
     CDS             893..2161
                     /locus_tag="PLV77_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNC14735.1"
                     /translation="MTRLTSRSRITRSRIRVFCFGLAAALLLVLVPGGAAAKPARLPK
                     APAGKNFYSPPAKLIKGKPGTAIWIRKTTGLARPPLAGRVLTIAYRSTSIRDRATVMS
                     GTIALPKGEPPKGGWPIVTFGHVTTGSADDCAPSRVTAASTELERMVRSNEFVGYLLG
                     HGVAVARPDFEGIGTPGRHPYMIGRSLGRSMIDIIRAGRQLDIGLGRRWAAAGHSEGG
                     QAALFTAEIGLRRAPGLSFRGVSAFAPASHVSDFVELARSFDTPSPLTDGFSGLGALI
                     LEGAAIAEPSLWQEYRDGGLSAEALELMPDTQRLCLADLSGTGSFGSMPPGALEGPDG
                     PQVLPEVYAVLDRNDPRTLSLPGVPVRLDQGSKDLVVWQSQTAEVADVLESKGAEVNY
                     EVWPGADHTDITDLDYAASASADWLIDLLR"
     gene            complement(2353..2700)
                     /locus_tag="PLV77_02430"
     CDS             complement(2353..2700)
                     /locus_tag="PLV77_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC14736.1"
                     /translation="MGQHFLKPLFDRVVIKELEPDRMRNSGLVVPPGTSEPPPQHGIV
                     LAVGQGIDWWEAAGVRMPVRPGDHVVFPAQAGNWVEVDEERLLVCRVTELLGVLENAR
                     DPETPELGGEVLD"
     gene            2789..3364
                     /locus_tag="PLV77_02435"
     CDS             2789..3364
                     /locus_tag="PLV77_02435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC14737.1"
                     /translation="MDDDLTRGDGEDEGLSRRPDPEDLDPTRIKLRPVAVDHVGDLRW
                     IHERPEVSRWWDEPGEDFPWDEPDSTRLTIFVDGQIGGMIQYWEEDEPKYRHAGIDLF
                     IDPALHNKGIGTTAVREVARYLFEARGHHRLEIDPAAENLAAIRVYEKVGFKPVGLTQ
                     RAERDADGGGWHDSLLMDMLSTELLPAEQED"
BASE COUNT          610 a         1160 c         1137 g          558 t
ORIGIN      
        1 gtcgacgtgg tccagcgggc catggacggt ggcgcggtgg gcgtggtcgg ggtgctggcc
       61 gattacttcg actcgaagta ctacttcaac gaggaatacc gcaagctgca gatggacatc
      121 ccgggcatgt gggtgaccag gtcggaaggg gatgcgatcc gcgcccagct ttcggtttct
      181 cccgacgccg aggcaaagct cgtcatgaac ggcgagcgcg aggcggcccc ggcccgcaac
      241 gtgatcggct tcctgcccgg gcagagcagg acgaccctgc tgatctcttc gcatcacgac
      301 gcggtttggg acggcgcggt tgaagacggc tcgggcacct cggaggtact cgcgctggcc
      361 cgctacttcg gggcgctgaa gaagaagtac cggcccatgt ccctcatgtt cgcgaccatg
      421 gattcccatt tcaccggcta tcagggccac gccgcgttcc tcaagaagta cgtcgagaac
      481 gcgccggaag cggaacggcc gagggtcaat ttcgcgatcg agcacattgc ccgccacgga
      541 tacgtggacg agaacggcaa gctgaagatg accaacctgc cggagatcgc cggcatcctc
      601 gaaaacgtcg gcgagaagcc tctgaacgtg atcaggaagg tgatccccaa gtacgggctt
      661 gaccgcacgg tggtcctgcc ggcaaccttc ttcggggtgt cgctgccgac cgacgcttcc
      721 ggcgcctacc aggaggcggt cccggtggtc agcctgatca gcggaccgat ctacctctat
      781 gacaggcagg acacactgga caaggtctac aagccgcatc tgcgcccgat ggctctcgcc
      841 tacaaggaga tgattgcccg catgagccgt atggacccgg aggatctgcc cggtgacccg
      901 ccttacaagc cgtagccgga tcacccgcag ccgtatccgc gttttttgct tcggcctggc
      961 cgcagccctg ctgctggtcc tggtcccggg cggtgcggca gcgaagcccg ccaggctgcc
     1021 caaggccccg gccggaaaga acttctacag cccgcccgcc aagctgatca agggcaagcc
     1081 gggaacggcg atctggattc gcaagaccac cggcctggcc cggccgccgc tggccgggcg
     1141 ggtgctgacc atcgcctatc gatccacatc gatccgggac agggccacag tgatgtcagg
     1201 cacgatcgcc ctgccgaaag gcgagccacc gaagggcggc tggccaatcg tcacctttgg
     1261 ccacgtcacg accggttccg ccgatgactg cgccccgagc cgggtcacgg ccgccagcac
     1321 ggaactcgaa cggatggtcc gcagcaacga attcgtcggc tacctgctcg ggcacggagt
     1381 ggcggtggcc cggccggact tcgagggcat cggaactccc ggacggcatc cctacatgat
     1441 cggccgctcg ctgggccgat cgatgattga catcatccgg gccggtcgcc agctggacat
     1501 cggcctgggc aggcgctggg ccgcggccgg ccattccgag ggtgggcagg cagcgctgtt
     1561 cacggcggag atcggcctcc ggcgcgcgcc cgggctttcc ttccgcggtg tctccgcctt
     1621 cgcaccggcc agccacgtca gcgacttcgt cgagctggcc cgctccttcg acaccccctc
     1681 gcccctgacc gacggcttct ccggcctcgg ggcactgatc ctcgaaggcg ccgcgatcgc
     1741 cgagccgtcg ttgtggcagg agtaccgcga tggcggcctc agcgccgaag cgctggagct
     1801 gatgcccgac acccagcgac tttgcctggc tgacctcagc ggaaccggtt cgttcggttc
     1861 gatgccgccc ggtgcgctcg agggaccgga cgggccgcag gtgctgcccg aggtttacgc
     1921 ggtcctcgat cgcaatgacc cccgtaccct gagcctgccc ggggttccgg tccggctcga
     1981 ccagggatca aaggacctgg tcgtctggca gtcgcaaacc gcggaggtcg cggacgttct
     2041 tgagtcaaag ggggcggagg tcaattacga agtctggccc ggtgccgatc acaccgacat
     2101 caccgatctc gactatgcgg cgtccgcctc tgccgactgg ctgatcgacc tccttcgttg
     2161 aggcctcaac caatcgcgaa cttctttgtt tgcgggaccg aaactcccga acccttatcc
     2221 ggttataaga gcggttgtct gacctctgga aagacagccg ggctccaaac gggagggaac
     2281 caacaagcag ggaggcgctg agcccgatcc gggggggatc gggctcaagc gtttctctgc
     2341 tctcccgata attcagtcga ggacttcgcc gccgagctcc ggggtttccg gatccctggc
     2401 gttctccaga acacccagca gctcggtgac ccggcagacc agcagccgtt cttcgtcaac
     2461 ctcgacccag ttgccggcct gggccggaaa gacgacatgg tcaccggggc ggaccggcat
     2521 cctcaccccg gccgcttccc accagtcgat gccctgaccg accgccagca cgatcccgtg
     2581 ctggggtggc ggctcggagg tgcccggcgg aacgaccagc ccggagttcc gcatccggtc
     2641 cggctcgagt tccttgatca ccacccggtc gaagagcggc ttcagaaaat gctgacccat
     2701 gacccaagcc tacgggctgc cggaaattcg ggttgcgaag cgggcaggcc cggttcccag
     2761 aggccaatac cggtagcctg cggccatcat ggatgacgac cttacccgcg gggacgggga
     2821 ggacgaaggg ctctcgcgcc gcccggaccc ggaggacctc gacccgacga gaatcaagct
     2881 caggccggtt gccgtggacc atgtcggtga cttgcgctgg attcacgaac gccccgaagt
     2941 ctccaggtgg tgggacgagc cgggcgagga ctttccctgg gatgaacccg attcgacccg
     3001 gttgacgatt ttcgttgacg gccagatcgg cgggatgatc cagtactggg aagaggacga
     3061 gcccaagtac cggcacgccg gcatcgacct cttcatcgat ccggcgctgc acaacaaggg
     3121 catcggcacc accgcggtcc gggaggttgc caggtacctg ttcgaggccc ggggccacca
     3181 ccggctcgag atcgacccgg cggcggagaa cctcgcggcg atcagggtgt acgagaaggt
     3241 cggcttcaag ccggtcggcc tgacccagcg ggccgagcgg gatgccgacg gcgggggctg
     3301 gcacgacagc ctgctgatgg acatgctctc gaccgagctg ctgccggccg aacaggaaga
     3361 ctgaccgccg gcgcctttgg cgctacccgg ccaggaagag gagaagggtg ctctcctgcc
     3421 ggctgcccgg cacggttacg gtcaccctca ccctgacctt ctcgt
//
LOCUS       DAOQMF010000096         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_14674_length_3425_cov_3.039466, whole genome shotgun sequence.
ACCESSION   DAOQMF010000096 DAOQMF010000000
VERSION     DAOQMF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14674_length_3425_cov_3.039466"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLV77_02440"
     CDS             <1..1044
                     /locus_tag="PLV77_02440"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC14738.1"
                     /translation="DVYVPKADSAKPRPVFVWIHGGGWFTGDKAGGTMEYKAKTLVDN
                     GFIFVSVNYRLSPDLAGPKALSKGRLIFPAHYLDAARALGWVNKHIGQYGGNPKKMVI
                     GGDSAGGQISSLLATKPAFLKARGMSTRQIKGVLALDAVGYDVARMMTPAYRRINSGF
                     QRMMFNAFGTPAEEKRNHRWAAASPINFAGRTDPPIFFVVPTTSPDRWVDAHVMARKL
                     GQRLGLVSHRVKTEHAGVVPLLGNPSGDMGVTAPLMRFAHAAVNPVRSTPTVRGTRIV
                     RAGRDGYGTFRLKFSSKPAARALICQVDRGVQSVCPVAMKSPPGLHRLRVWAYGNTGR
                     AAGLTTVKLRVIR"
     gene            1041..1904
                     /locus_tag="PLV77_02445"
     CDS             1041..1904
                     /locus_tag="PLV77_02445"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC14739.1"
                     /translation="MSRSIRVLLLVAVAGLFALSGIATQASARTYEPSFAAVSTDKGP
                     VFREGCLIYTKRATSPPCRYGEVGSSRKVTVLGDSHALQWTPPLINLARERGWELTML
                     LHANCTAALVNIDPYCNRWRHNALKRIRREKPTLVFVASNTAPSTFVMKNGRRLGRAA
                     SEPLFRQGMYETLLKLRKYGAQVTVMRDLPMSKAFLPSVCVSENVDQPGRCTFPARRP
                     LSQAYDFAAAKRLKAVQIIDPLPKVCPGGKCRAVHGNVLKYRDRGHISATYARLLTNW
                     LNGQLQNPFKA"
     gene            1981..3183
                     /locus_tag="PLV77_02450"
     CDS             1981..3183
                     /locus_tag="PLV77_02450"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC14740.1"
                     /translation="MAGLMLVAVPAAAEPARVITDINYNADEVGPAQSTSNSLDLYLP
                     ATDDGFEGLRPVVVWVHGGAWMTGDKSNRMTDKVNLFNSLGYIVASLNYRLSPDISEG
                     SFDGTWDPTRVRSPDHIADVAEGIAWVSNNVRHYGGDPDRLVLAGHSAGAHLVSLAGT
                     SPAWIKGRRVSPKQIIGVVSLDTDTFDVAASADPANPANSSQQLALIWNAFGTPDEEA
                     VEARWERSSPLAFADPADPPFLLVTQGEKVLRMAANDEMATALGQDPAQSVVGVPLDH
                     EGINEALGSITDGTVETARVSQFVRKVAEGARPAGVRMVRRPARTIVVKRRGALRPGR
                     QVRFGFVGTGRASGFQCRIDRQPFTRCRSPRDYRLKPGKHVFRVRALYPSGRPGNERT
                     VTFRIVRR"
BASE COUNT          586 a         1157 c         1098 g          584 t
ORIGIN      
        1 gacgtctacg tcccgaaggc cgattcggcc aaaccgcggc cggtcttcgt ctggattcac
       61 ggtggcggct ggttcaccgg tgacaaggcc ggtggaacca tggaatacaa ggcgaagacc
      121 ctggttgaca acggattcat cttcgtttcg gtcaactacc ggctttcccc tgatctggcc
      181 ggcccgaagg cgctctcgaa gggccgtctg atcttcccgg ctcattacct ggacgctgcc
      241 cgcgcgctgg gctgggtcaa caaacacatc ggccagtacg gcggaaatcc gaagaagatg
      301 gtgatcggcg gtgattcggc cggcggacag atctcgagcc tgctggcgac caagcccgcg
      361 ttcctcaagg cgcgggggat gtcgacccgt cagatcaagg gcgtgctcgc gctcgatgcg
      421 gtcggctacg acgttgcgcg gatgatgacg ccggcctacc ggaggatcaa ctcggggttc
      481 cagcggatga tgttcaacgc tttcggaact cccgcggagg aaaagaggaa tcaccgttgg
      541 gcggccgctt cgccgatcaa cttcgccggc cggactgatc cgccgatctt tttcgtggtc
      601 ccgaccacca gcccggaccg ctgggtggat gcccacgtga tggccagaaa gctgggccag
      661 cgactgggcc tcgtctccca ccgggtcaag accgaacatg ccggcgtcgt gccgctgctc
      721 ggcaatccgt ccggtgacat gggagtcacc gcaccgctga tgcggttcgc ccacgcggcg
      781 gtcaacccgg tccgctccac cccgaccgtc cgcggtacgc ggatcgtgag ggccggacgg
      841 gacggatacg gaaccttccg gctgaagttc agctcgaagc cggccgcccg ggccctcata
      901 tgccaggtcg accgcggcgt ccagtcggta tgtccggtcg cgatgaagtc gccgccgggt
      961 cttcaccggt tgcgggtctg ggcctatggc aacaccggtc gcgctgccgg tctgacgacg
     1021 gtcaagctgc gggtgatccg gtgagccgtt caatccgtgt tctgctgctt gtggccgtgg
     1081 ccggcctttt cgcgctttcc gggatcgcca cgcaggcttc ggcccgcacc tacgaaccct
     1141 cgtttgccgc ggtaagtacc gacaagggcc cggtcttccg cgagggttgt ctgatctaca
     1201 caaagcgggc cacctcgccg ccctgccgtt acggcgaggt cggttccagc cgcaaggtga
     1261 cggtcctcgg cgactcgcat gccctgcagt ggactccacc gctgatcaac ctcgccaggg
     1321 aacggggctg ggagctgacc atgctgcttc atgccaactg cactgccgca ttggtcaata
     1381 tcgatcccta ctgcaaccgc tggcgtcaca acgcactgaa gcggatcagg cgggagaagc
     1441 cgaccctggt tttcgtcgcg agcaacaccg cccccagcac cttcgtgatg aagaacggca
     1501 ggcggctggg ccgggcagcc agcgaacccc ttttccggca ggggatgtac gagaccctgc
     1561 tgaagctgcg caagtacgga gcgcaggtga cggtgatgcg tgacctgccc atgtcaaagg
     1621 ccttcctgcc ctcggtctgc gtttcagaga acgtcgacca gcccggacga tgcaccttcc
     1681 cggcccgccg gcccctttcc caggcctacg atttcgccgc tgcgaaacgg ctcaaggcgg
     1741 tgcagatcat cgatccgctg cccaaggtct gccccggagg caagtgccgt gccgtccacg
     1801 ggaacgtcct caagtaccgc gaccgcggcc acatcagcgc cacctacgcc cgcctgctga
     1861 ccaactggct gaacggtcag cttcagaatc cgttcaaggc ctgaggccgc tccgttggcc
     1921 ccggagccgg gttttccggg tatgaccggt cggatcacag ccgcaatctg ttcgctcgcg
     1981 atggcgggtc tgatgctggt cgcggttccg gccgcagccg aaccggcaag ggtaatcacc
     2041 gacatcaact acaacgccga cgaggtgggg ccggcgcaat cgacctcgaa cagtctcgat
     2101 ctctacctgc cggccaccga cgacggcttc gaaggtctgc gtccggtcgt ggtctgggtt
     2161 cacggcgggg cctggatgac cggggacaag tcaaaccgga tgaccgacaa ggtgaacctg
     2221 ttcaactcgc tcggctacat cgtggccagc ctcaactacc ggctctcgcc cgacatatcg
     2281 gagggcagct ttgacggaac ctgggacccg accagggtcc gctcgccgga tcacatcgcc
     2341 gacgttgccg aagggatcgc ctgggtgtcg aacaacgtcc gccactacgg cggcgacccc
     2401 gaccggctgg tcctggccgg gcattcggcg ggagcccatc tggtttccct ggccgggacg
     2461 agcccggcct ggatcaaggg ccgccgggtc tcaccgaagc agatcatcgg ggtcgtttca
     2521 ctggacaccg acaccttcga cgtggccgct tcggcggacc ccgccaatcc ggccaattca
     2581 agccagcagc tcgccttgat ctggaatgcc ttcggcacac cggatgaaga agcggtcgag
     2641 gcacgctggg aacgctcctc gccactcgcc ttcgccgatc ccgctgaccc gcccttcctg
     2701 ctggtaaccc agggggaaaa ggtcctgcga atggccgcca acgacgagat ggcgaccgcc
     2761 ctcggccagg accccgccca atcggtggtc ggggtgccgc tcgaccacga ggggatcaac
     2821 gaggcgctgg gttcgatcac cgacgggacc gtcgaaaccg cccgggtgag ccagtttgtc
     2881 cggaaggtcg cggaaggcgc caggccggcc ggggtcagga tggtccggcg cccggccagg
     2941 acgatcgtgg tcaagcgccg cggggcccta aggcccgggc gccaggtcag gttcggcttc
     3001 gtcggcaccg gccgggccag cggcttccag tgccggatcg accgccagcc gtttactcgc
     3061 tgccgcagcc ctagggacta ccgcctgaaa cccggcaaac acgtcttccg ggtgcgagcg
     3121 ctctacccga gcggaaggcc gggcaacgag cggacagtga ccttccgcat cgtgcgccga
     3181 taggcggagc ccctcccaac cgtcattttc ttcgagtggg aggggcgggt tcgaattgaa
     3241 gcgccggcga agaccggcgc gactgcagtt aagttcagaa gccgtattgc tccttgagct
     3301 ccggatcctt ctcggccagc atctcggcca ggccgaccat caccttgcac tgcttccagg
     3361 tggcgtcgtc ctgcatcttg ccgtcgatca tgtgaacacc gcggccgtcc gggatcgcct
     3421 cgatc
//
LOCUS       DAOQMF010000097         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_14838_length_3404_cov_3.676620, whole genome shotgun sequence.
ACCESSION   DAOQMF010000097 DAOQMF010000000
VERSION     DAOQMF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14838_length_3404_cov_3.676620"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLV77_02455"
     CDS             complement(<1..183)
                     /locus_tag="PLV77_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14741.1"
                     /translation="MPEPREKSANKASAGLPATLVLTLSVTAGIFLLLLAPVYLLLPT
                     TPMPEPLPPQHQNAETL"
     gene            238..1515
                     /locus_tag="PLV77_02460"
     CDS             238..1515
                     /locus_tag="PLV77_02460"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC14742.1"
                     /translation="MRDSTPRPGDRRLEESEREAGQVLPPPPGHPRFPLLDPMRAIAA
                     ISVMLVHVAIFSGGFGPWYKWVLAHLEIGVPFFFLLSGFLLYRPMLAARVSGLPAQRI
                     RDYARNRFFRIFPLFWFILIVTAIVPGMYGAFTGNWWVYFGLLQNLPVYSPDGECVTN
                     PFRCGIPPSWTLGIEVMFYFLLPFFAAGMTMIQNRFRSTPSGGFFSRWVKIEFAALLL
                     FSAISFVIQSSSFNGGVDQWLFFSPIGRAWWFALGMVLAVISVRTAETGRDVALSRWG
                     REHGGWCWLAAIGLYLFGTYVLFSHGPTLAAPVGDLNEYLLQYFFFGVISFLALMPAV
                     FGQIGSGVATRVLAHPVPVRLGLISYGIFLWHYPVLVALNDLDATSWATSAQFPVMAI
                     ATFVLTVALSLITYYLLERPLMRWSRSRSKKRA"
     gene            complement(1554..2336)
                     /locus_tag="PLV77_02465"
     CDS             complement(1554..2336)
                     /locus_tag="PLV77_02465"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HNC14743.1"
                     /translation="MKGRILVMGGHEFDRLDGNEAIVEHVISLTGMKAPKICLLPTAS
                     GDPEDQISRFRRSFGSRGCEVSDISLFRLGANPIDVSAHLMKQDAIYVGGGSLVNLVA
                     VWRPHGIAGLIEECLERGVMVVGQSAGAMCWFEAGITSSSGRPEPAEGLGVLKGSLCV
                     HYHRDPERRTRYLAEVGKSMSAGYGADDQTGLLFTDGELTEAFTAREGAGVWKVTPAA
                     KGGVEEPVEAVDIRPKSKPDTPGDPVQDFRNLSKWRQSATIR"
     gene            complement(2371..3141)
                     /locus_tag="PLV77_02470"
     CDS             complement(2371..3141)
                     /locus_tag="PLV77_02470"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HNC14744.1"
                     /translation="MGTRRQIVAFGGGGFSMESGNPLLDDFVVQATGLKKPRVCFMPT
                     ASGDADHYLVRFYNAFRGRGEASHISLFRRERGAESIRDHLLSQDLIYVGGGSVISML
                     GVWRAHGIDRILREAWERGIVLCGLSAGSLCWFEECVTTYQKETRVTIGLGLLPWSNQ
                     VHFELGSTAHKAFEEKLKEGLRPGYAATDGAAFHFIDTELAQVVASRPEARAYRIELK
                     GDHPKTTQLATRYLGSPDQVPLVEESPIPWASTPIEAA"
BASE COUNT          518 a         1186 c         1048 g          652 t
ORIGIN      
        1 cagggtctcg gcattctggt gctgcggcgg cagcggctcc ggcatcgggg tggtcggcag
       61 caggagatag accggcgcaa gcagaagcag gaagatcccg gccgtgaccg agagggtcag
      121 gaccagggtc gcgggcagcc cggctgacgc cttgttggcg gacttttccc tcggctcggg
      181 caccgggcga tcctacctgc gtcaaccggt tctccccggt ctatagtccc tgcccgaatg
      241 cgagattcga ccccgcggcc gggcgacaga aggcttgagg agtcggagcg cgaggccggg
      301 caggttctgc cgcccccgcc cggccacccc cgtttcccgc tgctcgaccc gatgcgcgcg
      361 atcgccgcga tctcggtgat gctggtgcat gtcgccatct tcagcggagg tttcggcccc
      421 tggtacaagt gggtgctcgc ccacctggaa atcggcgttc ccttcttctt cctgctctcg
      481 ggcttcctcc tctaccgccc gatgctggcc gcccgggtct ccgggcttcc ggcccagcga
      541 atccgcgatt acgcccgcaa ccgcttcttc cggatctttc ccctcttctg gttcatcctg
      601 atcgtgaccg cgatcgtgcc cgggatgtac ggcgccttca ccggcaactg gtgggtctac
      661 ttcgggctgc tgcagaacct tcccgtgtat tcccccgacg gcgaatgcgt gaccaacccc
      721 ttccgctgcg gcatcccgcc ttcctggacg ctcgggatcg aagtcatgtt ctacttcctg
      781 ctgcccttct tcgcggccgg catgacgatg atccagaacc gcttccgcag cactccctcg
      841 ggcgggttct tttcccgctg ggtgaagatc gagttcgcgg cgctgctcct cttctcggca
      901 atctcgttcg tgatccagtc ctccagcttc aacggcgggg tcgaccagtg gctcttcttc
      961 tccccgatcg gccgggcctg gtggttcgcc cttggcatgg tcctcgccgt gatctcggtg
     1021 cgaacggcgg aaaccggccg ggacgtggcg ctctcgcgct gggggcgcga acacggtggc
     1081 tggtgctggc tcgccgcgat cggcctctac ctcttcggca cctatgtcct gttcagtcac
     1141 gggccgaccc tggccgcccc ggtcggggac ctgaacgagt atctcctcca gtacttcttc
     1201 ttcggcgtga tctcgttcct cgccttgatg ccggccgtct tcggccagat cggcagcggg
     1261 gtggcgacca gggttctcgc ccatcccgtc ccggtgcgcc tcgggctgat ctcctacggg
     1321 atcttcctct ggcattaccc ggtgctggtc gcactcaacg atctcgacgc gaccagctgg
     1381 gccacctccg cgcagttccc ggtcatggcg atcgcgacct tcgtcctgac cgtcgcgctc
     1441 tccctgatca cctactacct gctcgagcgc ccgctcatgc gctggtcccg cagccggtca
     1501 aagaagcgcg cctgaccgac ggacacccgg aacgtggcgc cgtcttcacg ggttcatctg
     1561 atggttgccg actggcgcca cttacttaga ttgcggaagt cttggaccgg gtcgccgggg
     1621 gtgtccggct tcgacttcgg ccggatgtcg accgcttcga ccggctcttc gaccccgccc
     1681 ttcgcggccg gggtcacctt ccacaccccg gctccctccc gggctgtgaa ggcctcggtc
     1741 agttcaccgt cagtgaagag caggccggtc tggtcgtcgg caccgtaacc ggccgacatc
     1801 gacttgccga cctcggccag gtagcgggtc cgccgttccg gatcccggtg gtaatgaacg
     1861 cagaggctgc ccttcagcac accgagaccc tccgccggct cgggccgccc cgaggacgag
     1921 gtgatgccgg cctcgaacca gcacatcgcg ccggcactct ggcccaccac catcacgccg
     1981 cgctcgaggc attcttcgat cagcccggcg atcccatgtg gcctccagac cgcgaccagg
     2041 ttgaccaggc tgcccccgcc gacgtagatc gcgtcctgtt tcatcaggtg cgcggaaaca
     2101 tcgatcggat tcgcaccgag ccggaacagc gagatgtcac tgacctcgca accgcgactg
     2161 ccgaaggacc ggcgaaagcg ggagatctgg tcttccgggt cccccgatgc ggtcggcagc
     2221 aggcagatct ttggcgcttt catcccggtc agcgagatga cgtgctcgac gatcgcctcg
     2281 ttgccatcga gacggtcgaa ctcgtgacca cccatgacca ggatccggcc cttcatcgca
     2341 gaacccgctt gcggtttcgg ttgcctcgca tcaggccgct tcgatcgggg ttgacgccca
     2401 gggaatcggt gactcctcga ccagcggcac ctggtcgggt gagccgaggt acctcgtcgc
     2461 gagctgggtt gtcttggggt gatcgccctt gagttcgatc cggtaggccc gggcctcggg
     2521 ccgggaagcc acgacctgcg cgagttcggt gtcgatgaag tgaaaggcgg caccatcggt
     2581 cgccgcgtac cccggacgca ggccttcctt cagcttttcc tcgaacgcct tgtgggcagt
     2641 cgagcccagt tcgaagtgaa cctggttcga ccagggcagc agaccgaggc cgatcgtcac
     2701 cctggtttcc ttctggtagg tggtcacgca ttcctcgaac cagcagagcg agccggctga
     2761 aagtccgcag aggacgatgc cgcgctccca ggcctcgcgc agaatccggt ctatgccgtg
     2821 ggcgcgccag accccgagca tgctgatcac gctgccaccg ccgacgtaga tcaggtcctg
     2881 ggagagcagg tggtcacgga tcgattccgc cccgcgctcc cgccggaaga gagagatgtg
     2941 cgaggcctcg ccgcggcccc gaaaggcgtt gtagaagcgg accaggtaat ggtcggcgtc
     3001 gccggaagcg gtcggcatga agcagacccg gggcttcttc aggccggtcg cctgcaccac
     3061 gaagtcatca agcaggggat tgccggattc catcgagaac ccaccaccac caaacgccac
     3121 gatctgtctc cgcgttccca tggagaaata cttcccgctc cccaccagtt cccatttggc
     3181 caccgggcca gactctgaac acagtcgttt gtccaaccgc ggatgtagcc gtcccatcca
     3241 ttccttgttt ctctgggacg gcggggttca ctgggagggc gggtccgacc ccgcccgact
     3301 tgcaggtact cgtggggtcc ccctgtttga aaaccgctcg atttgggcaa tgaaagcgac
     3361 atcaggcgcg cgcaaaccgg gcgcaaaagg gaaccccgtc actc
//
LOCUS       DAOQMF010000098         3402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_14859_length_3402_cov_3.700030, whole genome shotgun sequence.
ACCESSION   DAOQMF010000098 DAOQMF010000000
VERSION     DAOQMF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14859_length_3402_cov_3.700030"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLV77_02475"
     CDS             complement(<1..683)
                     /locus_tag="PLV77_02475"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HNC14745.1"
                     /translation="MKIGVPRESAEGENRVALVPESVKSLLKKEGVEIVIESGAGDVA
                     GHPDSQYEEAGAKVGSHADALAADLVLHVAPLSSAQIGELKQGQYLISHLSPLTSAET
                     NNALASAGVTALAMELIPRTTRAQAMDALSSQATAAGYAATLIAARESGSFWGMLTTA
                     AGTIRPARVLVLGAGVAGLQAIATAKRLGAIVTGFDIRRAAWEQIASLGGRPLELDAR
                     EAARRSERE"
     gene            complement(771..1820)
                     /locus_tag="PLV77_02480"
     CDS             complement(771..1820)
                     /locus_tag="PLV77_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNC14746.1"
                     /translation="MSLKLGLYLGYWGIGPRGKDAIEAVQFAESIGYESVWVAESYGS
                     DCVSVLAWLAAQTEKINLGAAIMQVPARPPAAAAMAGATIDYLSNGRFMFGFGPSGPQ
                     VSEGWYGVPYSKPWGRTKEYVEVVREIVAREEKLDHQGEHYQLPLPEGQGKPLKLNFK
                     PLRNDIPIFIGAIGRRSVEIAAEVADGWIPIFFSPEKFEEAWGEHIEKGLAAGGRTRD
                     DFEISPSVQVAIDGDLEAARNMVRMALVLYLGGMGSKKTNFYVDLSHRFGFGEVADEV
                     QELFQAGDKEGAFKALPDEFVNAVSLIGTEAEVADRLTAFREKGIDRLIMTPVHPELD
                     QMRHTVERLAALTQD"
     gene            1890..>3402
                     /locus_tag="PLV77_02485"
     CDS             1890..>3402
                     /locus_tag="PLV77_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC14747.1"
                     /translation="MTEAATQVLPADDPGRGLSGSEAAERLRSLGPAQPHSSRSTSSI
                     VAGNVFTLFNLIIGIFFVLILSLGLIADAIFGMIAIVNTWIGIRQEMKAKETLDRLAI
                     LVAPRAEVIRDGQPLELLADEVVPGDLVRISPGDQLVADGRMTSSRGLTVDESMLTGE
                     SDAIGKSPGDEVFSGSFVISGSGFCEVTAVREESYAGKIAGEAKAFRHPPSPLQEEVN
                     QVILVCTWVLLPLAALLIASLIVRQVGLVDAAQTATAGLITLIPEGLVLLMSVTFAVA
                     AVKMARRDTLVQQMSATESLASVDTICVDKTGTLTDGDLTVVEVIPAKVTDRKLAARE
                     LGRYAASAGDRNLTLEAIGEEFPAEAETVVGEVAFSSEWKWSALSFDEGGARYSLVLG
                     APDLMIGAGALTLDEPLAARLEEETGAGRRVVALCRTTSPLPASGLDGHPAGLEPIAL
                     VVLRENLRPGVEETLELMRSEDVALKLISGDAKATVTAVAAAVGIPTDAATAVT"
BASE COUNT          551 a         1146 c         1144 g          561 t
ORIGIN      
        1 tcctcgcgct cgctcctgcg agcggcctcc cgcgcgtcga gctcgagcgg gcgaccgccc
       61 agggaggcga tctgttccca ggcggcgcgg cggatgtcga aaccggtgac gatcgccccg
      121 agacgcttgg ccgtggcgat cgcctgcagc ccggcgacgc cggccccgag caccaggacg
      181 cgggccggac ggatcgtgcc ggcagcggtg gtgagcatgc cccagaagct gcccgactcg
      241 cgcgcggcga tcagagtcgc ggcataaccg gccgcggtcg cctgagagga aagtgcgtcc
      301 atcgcctggg cgcgggtggt gcgcggaatc agttccatcg ccagggcggt gaccccggcc
      361 gaggcgaggg cgttgttggt ctccgccgag gtcagcggcg agagatggct gatcaggtac
      421 tggccctgct tcaactcgcc gatctgggcg ctcgaaagcg gagcgacgtg aagcacgaga
      481 tcggccgcga gcgcgtcggc atgcgaaccg accttcgctc ccgcttcctc gtactggctg
      541 tctggatgcc cggcgacatc gccggctccg gactcgatga cgatctcgac gccttccttt
      601 ttcaggagcg atttgaccga ctcgggaacc agggcaaccc ggttctcgcc ttcagcagat
      661 tctctgggta caccgatctt catggacaag gccgactcag attatgggaa ttagccgcag
      721 aggggattcc cgaccggcgg acagccgccg gccggatcag atcctcttgg tcagtcctgc
      781 gtcagggcgg ccagccgctc gacggtgtgc cgcatctgat cgagctccgg gtggaccggg
      841 gtcatgatca gccggtcgat ccccttttcg cggaaggcgg tcagccggtc ggccacttcg
      901 gcctcggtcc cgatcagcga gaccgcgttg acgaactcgt cgggcagcgc cttgaacgcg
      961 ccttccttgt cgccggcctg gaagagctcc tgaacctcgt cggcgacttc accgaagccg
     1021 aaacggtggg agaggtcaac gtagaagttg gtcttcttcg atcccatgcc gccgaggtag
     1081 aggaccagag ccatgcgaac catgttccgg gccgcctcga ggtcaccgtc gatcgcgacc
     1141 tggacggaag gcgagatttc gaagtcatcc cgcgtgcgcc cgccggcggc gagccccttt
     1201 tcgatgtgct cgccccaggc ttcctcgaac ttctccggac tgaagaagat cgggatccag
     1261 ccgtcggcga cctcggccgc gatttcgacc gaacggcggc cgatcgcacc gatgaagatc
     1321 gggatgtcgt tgcgcagcgg cttgaagttg agcttcaagg gcttgccctg gccttcgggc
     1381 aggggcagct ggtagtgctc gccctgatgg tcgagcttct cctcacgggc gacgatctcg
     1441 cggaccacct cgacgtattc cttggtccgg ccccagggct tggagtaggg aacgccgtac
     1501 cagccctcgg aaacctgcgg gcccgagggg ccgaagccga acatgaagcg gccgttcgag
     1561 aggtagtcga tcgtggcgcc ggccatcgcg gcggcagccg gcgggcgggc cgggacctgc
     1621 atgatcgccg cgccgagatt gatcttctcg gtctgggcgg caagccaggc gaggacgctg
     1681 acgcagtccg atccatacga ctcggccacc cagaccgatt cgtagccgat gctttcggcg
     1741 aactgcaccg cctcgatcgc atcttttccg cggggcccga tgccccaata accgagatac
     1801 agcccaagct tcagtgacat gcgggcatcc tacccgaaag tacaaattct gtttttgttc
     1861 caaccgggca aaccggtaac aatcattccg tgactgaagc ggccacccag gtactcccag
     1921 ccgatgatcc gggccgcggc ctgtccggtt ccgaagcggc cgagcgcctg cgcagcctcg
     1981 ggcccgccca gccccactcc tcgcggtcga cctccagcat cgtcgccggc aacgtcttca
     2041 cgctcttcaa cctgatcatc gggatcttct tcgtcctgat cctctcgctc gggctgatcg
     2101 ccgatgcgat cttcggaatg atcgcgatcg tcaacacctg gatcggcatt cgccaggaga
     2161 tgaaggcgaa ggagaccctg gaccggctgg cgatcctggt cgccccgcgg gccgaggtga
     2221 tccgcgatgg ccagccgctg gagctgctgg ccgacgaggt ggtgcccggc gacctggtcc
     2281 ggatttctcc cggggaccag ctggtcgccg acggccggat gaccagctcc cgcggcctga
     2341 ccgtcgacga atcgatgctg accggcgagt ccgatgcgat cggcaagtcc ccgggcgacg
     2401 aggtcttctc cggcagcttc gtgatctccg gttcgggctt ttgcgaagtg accgcggttc
     2461 gggaggagag ctatgccggg aagatcgccg gcgaggcgaa ggccttccgc cacccgcctt
     2521 caccgctcca ggaagaggtc aaccaggtga tcctggtctg cacatgggtc ctgttgccgc
     2581 tggccgcgct cctgatcgcg tcgctgatag tccgccaggt cgggctggtc gacgccgccc
     2641 agaccgcgac cgccggcctg atcaccctga tccccgaggg cctggtcctg ctgatgagcg
     2701 tgaccttcgc cgtcgcggcc gtgaagatgg cccggcggga cactctggtc cagcagatga
     2761 gcgcgaccga atcacttgct tcggttgaca cgatctgcgt cgacaagacg ggcaccctga
     2821 ccgacggcga cctgaccgtg gtcgaggtga ttccggccaa ggtcaccgac cgcaagctcg
     2881 ccgcccgcga gctcggccgc tacgccgcca gtgccggtga ccgcaacctg accctcgagg
     2941 cgatcggcga ggagttcccg gccgaggccg aaacggtggt cggcgaggtg gccttctcat
     3001 ccgagtggaa gtggagcgcc ctgagcttcg acgaaggggg tgcccgctac agcctggtgc
     3061 tcggggcgcc ggacctgatg atcggggccg gcgcgctgac cctggatgaa ccgctcgccg
     3121 cccgcctgga agaagagacc ggtgccggcc gtcgggtcgt ggccctctgc cggacgacct
     3181 ccccgctgcc ggccagcggc ctcgacggac acccggccgg gctcgagccg attgccctgg
     3241 tggtcctgcg cgagaacctc aggccggggg tcgaggaaac cctggagctg atgcgaagcg
     3301 aagacgtggc cctcaagctg atctcggggg acgccaaagc gacggtcacc gcggtcgccg
     3361 cagcggtcgg gatcccgacc gacgcggcga ccgcggtgac cg
//
LOCUS       DAOQMF010000099         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15045_length_3377_cov_3.331126, whole genome shotgun sequence.
ACCESSION   DAOQMF010000099 DAOQMF010000000
VERSION     DAOQMF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15045_length_3377_cov_3.331126"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLV77_02490"
     CDS             <1..334
                     /locus_tag="PLV77_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNC14748.1"
                     /translation="DEVIGSETKVKVVKNKVAPPFKETLFDILYGEGTSREGEIIDLG
                     VQHKFVDKSGAWYAYGGEKIGQGKDNAREFLKSNPHIAREIENKIRVAVGLPEMAVIP
                     PASSAEAA"
     gene            363..923
                     /locus_tag="PLV77_02495"
     CDS             363..923
                     /locus_tag="PLV77_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNC14749.1"
                     /translation="MGTGLHERALRCLAQREHSRAELARKLSSLGSDEEVSAELDRLV
                     ELGLLSDARFADAYVRAKAARFGTARLTSELSRRGVGAEQIGLAIADNCTESEFERAS
                     DVHRRKFSGPGGVGRNFDRGRVAALQELGLQQRAARVDIGQQPAIPIPQLIVEDQLHG
                     RTPVKQLLVELLGLWPVALDRVTLLD"
     gene            complement(1208..2128)
                     /locus_tag="PLV77_02500"
     CDS             complement(1208..2128)
                     /locus_tag="PLV77_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14750.1"
                     /translation="MEANTQTEQKSLLATLPPGKRARLHRLLFEFGPGNGTMMLLPID
                     QGIEHGPRDFFPNPASKDPEYQFKLAAEAGYSALACQIGMAEKYYPDYAGEVPLILKV
                     NGKTDIPSSAHAFSACNATVEDGVRLGADAIGYTLYVGSPRQDEDLAQLRQVRQDCDR
                     FGMPLVVWSYPRGEAIDAKGGAGSFYAIDYAARMAMEMGADVVKLNYPKIDPEKDKDA
                     QPPYNEMDVTAEEAMRQCVESAGRSLVVLSGGSKTDDETVLGHCKSVMNAGGSGVIFG
                     RNVWQREWSEALEIIAQIKETLLANVKRTP"
     gene            2242..3039
                     /locus_tag="PLV77_02505"
     CDS             2242..3039
                     /locus_tag="PLV77_02505"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC14751.1"
                     /translation="MSETELNVVCKSCGAEVSPYVTECPYCGARLRKRAPDLELGGDG
                     GLEPKQSRREKRREEKARQKAARRSGPRPARAASLGVRPWATISLILIPAVALIVRIA
                     LGNALEDFGGVIVPVQSEWWRILTAPFAYLSVGYLFAVGLALALFGPGIERRFGTVPT
                     FLLLVACGALGVLAASAVADQREVLTVIAGGNGIALGAIGAWFMVARSESRATGDGFE
                     AAGAIVSAAVILLLPVVVPTADFWSGLAGGIVGVLAGFTATRFGSPR"
     gene            3057..>3377
                     /locus_tag="PLV77_02510"
     CDS             3057..>3377
                     /locus_tag="PLV77_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14752.1"
                     /translation="MPEEQISDEQLAAAVERLSDPERFREAEQLVSRVAPGLPAVLVT
                     ALGSGGWFGESHLSETLKAATIPDEDQRLTAIRALLTEETQMGMMVGVAVGWALRQEL
                     ENGDT"
BASE COUNT          567 a         1102 c         1125 g          583 t
ORIGIN      
        1 cgacgaggtg atcggctccg aaaccaaggt caaggtcgtc aagaacaagg tcgcaccgcc
       61 gttcaaggag accttgttcg acatcctcta cggcgaaggc acctcccgcg agggcgaaat
      121 catcgacctg ggcgtgcagc acaagttcgt cgacaagtcc ggcgcctggt acgcctatgg
      181 tggcgaaaag atcggtcagg gcaaggacaa cgcccgtgag ttcctcaaga gcaaccccca
      241 catcgcccgc gagatcgaaa acaagatccg cgtcgccgtc gggctgcccg agatggccgt
      301 gattccgcct gcctcctcag ccgaagctgc ctgaacctcg ccggcgagga aagcggtcgt
      361 ccatgggtac cggtctgcat gagcgagcct tgcgttgtct ggctcaacgg gaacactccc
      421 gcgcagaact tgcacgcaag ctgagcagcc tcggcagcga cgaggaagtg agtgccgaac
      481 tcgaccggct ggtcgaactc ggcctcctct ccgacgcccg cttcgccgac gcctacgtcc
      541 gcgccaaggc agcccgcttc ggcaccgcgc ggctcaccag tgaactcagc cggcggggcg
      601 tgggcgctga gcagatcggc ctggccatcg cagataactg caccgagagc gaattcgaac
      661 gcgcctccga cgtccatcgc cgaaaattct ccggcccggg aggtgttggg cggaacttcg
      721 atcgtggtcg cgtagccgcc ctccaggagc tcggcctgca gcagcgtgcg gcccgagtag
      781 acataggcca gcagccggcc ataccgatcc ctcagctcat cgtcgaagac cagcttcacg
      841 gtcgaacgcc ggtcaagcag ctcctcgttg aactgcttgg cctctggccc gtagcactcg
      901 atcgggtaac cctccttgac tgattccggg gtgtcgatcc cgatgtagcg aacgtattgc
      961 tggtggccgt ccatccagac cagggcagtg tcgccatcca ccacccggtc gacatcgacc
     1021 ctgagaacag aagacccgga gccttgaagc cccgggtcct tgttgccgtc gtcccggttc
     1081 agcagccagt aaccgaaggc cgcgaggatg acgacgaaga gaatcagata cccccggctg
     1141 tggtccggga agaggttccc gaactggcca gggggtgtcg gaatgttccc ctgtttcaaa
     1201 cgacccctta cggagtgcgc ttgacgttgg cgagcagcgt ctccttgatc tgggcgatga
     1261 tctccagggc ctcgctccat tcgcgctgcc agacgttgcg gccgaagatc acgccggaac
     1321 cgccggcatt catcaccgac ttgcagtgac cgagcacggt ctcgtcgtcg gtcttggatc
     1381 cgccggagag cacgaccagc gaacggccgg ccgactcgac gcactggcgc atcgcctctt
     1441 cggcggtcac gtccatctcg ttgtacggag gctgcgcgtc cttgtccttc tccgggtcga
     1501 tcttcgggta gttgagcttg accacgtcgg cgcccatctc catcgccatg cgggccgcgt
     1561 aatcgatcgc gtagaaggaa ccggcgccgc ccttggcgtc gatcgcctca ccgcgcgggt
     1621 aggaccagac gaccagcggc atgccgaagc ggtcacagtc ctggcggacc tggcgcagct
     1681 gggcgaggtc ctcgtcctgc cgcggcgagc cgacgtagag cgtgtagccg atcgcgtcgg
     1741 cgccgagccg gacgccgtcc tcgacggtcg cgttgcaggc ggagaaggcg tgggcggagg
     1801 agggaatgtc ggtcttgccg ttgaccttga ggatcagcgg tacctcgccg gcgtagtccg
     1861 ggtagtactt ctcggccatc ccgatctggc aggcgagggc ggagtaaccg gcctcggcgg
     1921 ccagcttgaa ctggtactcg ggatccttcg aggcggggtt cgggaagaag tccctgggcc
     1981 cgtgctcgat gccctggtcg atcggcagca gcatcatggt tccgttaccc gggccgaact
     2041 cgaaaaggag ccggtgaagc cgggcgcgct tgcccggagg cagcgtcgcg agcagtgact
     2101 tctgttcggt ctgggtattg gcttccattg gcttctcggt cctcctgggg gattggaaaa
     2161 agtctcgaca ggcagtatgt caatgccgga gccgatcagg caggcaggtt ttggcccgca
     2221 ggttctatcc tcggtgatgc gatgagtgag accgaactca acgtggtctg caagtcatgt
     2281 ggggcggaag tcagccccta cgtcaccgaa tgcccgtatt gcggcgcccg gctgcgcaaa
     2341 cgcgcccctg atctggagct gggaggagac ggcggactcg agcccaaaca gtcgcgccgc
     2401 gagaagcgcc gggaagagaa ggcccggcag aaggcagccc gacggtccgg gccccggccg
     2461 gcccgggcgg cttccctcgg cgtccggccc tgggccacca tttccctgat cctgatcccc
     2521 gcggtggcct tgatcgtgcg gatcgcgctg ggcaacgcgc tcgaggactt cggcggggtg
     2581 atcgtgccgg ttcagagcga gtggtggcgg atcctgaccg ccccgttcgc ctatctgagc
     2641 gttggctacc tcttcgcggt cggtctcgcc ctggctctgt tcggcccggg catcgaacgc
     2701 cgcttcggga ccgtgccgac ctttctgctc cttgtggcct gcggcgcgct cggagtgctc
     2761 gccgcgtcgg cggtggccga tcagagagag gtgctgaccg tgattgccgg cggcaacggc
     2821 atcgcgctgg gcgcgatcgg cgcctggttc atggtcgccc gatccgaatc ccgcgccacc
     2881 ggggacggtt tcgaggcggc cggagcgatc gtctccgccg cggtgatcct gctgctgccg
     2941 gtcgtcgtgc ctaccgccga cttctggagc ggcctggccg gcggaatcgt cggtgtcctg
     3001 gccggtttca ccgcgacccg tttcggctca ccccgctgac cctctaggct gacccggtgc
     3061 ccgaggagca gatctctgac gagcaactcg ccgccgcggt agagcgactg agcgacccgg
     3121 aacggtttcg cgaggccgaa cagctggtct cccgggtcgc cccgggattg cccgctgtac
     3181 tcgtcacggc cctcgggagc ggcggctggt tcggtgaatc acatctctcc gagaccctga
     3241 aggcggccac gatcccggat gaagaccagc ggctgaccgc gatcagggca ctgctcaccg
     3301 aggaaaccca gatgggaatg atggtcgggg tagcggtcgg ctgggccctg aggcaggaac
     3361 tggaaaacgg tgacacc
//
LOCUS       DAOQMF010000100         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15110_length_3371_cov_3.030760, whole genome shotgun sequence.
ACCESSION   DAOQMF010000100 DAOQMF010000000
VERSION     DAOQMF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15110_length_3371_cov_3.030760"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1302)
                     /locus_tag="PLV77_02515"
     CDS             complement(58..1302)
                     /locus_tag="PLV77_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC14753.1"
                     /translation="MAPALTLPLRNSVYRRLFSAQIVGLLGTGISTVALGLLAFSMGG
                     GNAGQILGVVLALKVATYVLVSPAIAAFAAGVSRKKLLVTMDVVRALAIGSIAFVGEI
                     WQVYVLIVIVSAASAGFTPAFQALIPDIFTDVDTYTEALSLSRLAYELEALVSPLIAG
                     LLLAVVGYTELFTLNSLAFLTSAGLVLTTAVPAVRDDALAARKLSQVSQGIRQFLRTP
                     RLQGLFLLDWAAAAAGAMVLINTVIYVKGQLGFGDSDVAWALGAFGAGSIVIALLLPT
                     LLRRFTDRSLMLLGGLILAASMFLFVAVGSLTTLMACWALAGIGTSAVLTPSGRLIQR
                     SGDSAGRPALFAAQFSLSHFCWLFTYLIAGFAGEALGLSEVALILGAIAAGASVLAAM
                     VWRPEPPARQASSTGSPQRLSR"
     gene            complement(1311..2537)
                     /locus_tag="PLV77_02520"
     CDS             complement(1311..2537)
                     /locus_tag="PLV77_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNC14754.1"
                     /translation="MANGTPMKLAAPHGEADQNWLRARLEELERIDRPSASAGERAAA
                     EWLVSRFSELGAEARIETEKAHGTYWWPIGIGTALGAAGGIAATRGRRLAGALLGAAG
                     AAAIATDFPPRPRYLRRFLPKRDTFNVVCELGDPEAERTVVFVSHHDAANAGLVFHPG
                     IPDLVSKTGAFQHLDTSPMLMAPVIGGPLLSAIGGLTGSRKLAAAGLLLSAGSTASMV
                     DIGLRKVVPGANDNGTAVIALLELARRFLADPPEGLRLILLSAGSEESFSEGMKAFGE
                     RHFPNLPVDSTFFINMDTIGSPYLTVLRGEGFLKMFEYPPEALILADRAAEEEGIELM
                     PNLRIHNGTDGLEALAAGYPVVSICSCTEHKQPANYHWPNDVSENVDFDTVESGIRLA
                     EAMARRLSRDWVSLPG"
     gene            2599..>3371
                     /locus_tag="PLV77_02525"
     CDS             2599..>3371
                     /locus_tag="PLV77_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNC14755.1"
                     /translation="MFGGANSITLFRVAGIRIAVDWSWFIFLFIVIVWLSGFYGDILD
                     EPGSTQSYGLALASAIGFFGSILLHELGHAFEAKRSGIGIRTIRLWLFGGVAEMDQES
                     ESPGTEFRVAIAGPVVTLLIAVILCVIGIQAAGFDNFRDALEMRITAETNGPITMVAW
                     LAAVNILVLGFNLLPAFPMDGGRIVRSIAWKITGKRSAATRFAAGLGRIFGFIFIGLG
                     LLWIFNGNLFSGLWLAMIGFLVNSSARAASRSTVGDKLEG"
BASE COUNT          649 a         1058 c         1117 g          547 t
ORIGIN      
        1 cgcgccgcgc ggccggcagc gagccggtat gtgcgtcgag gaccagcgcg accgcgatta
       61 ccgggacaac cgctggggcg accccgtaga cgaggcctga cgcgcaggcg gctcgggccg
      121 ccacaccatc gctgccagga cactcgcgcc ggcggcgatc gcgccgagaa tcagggcaac
      181 ctcggaaagc cccagagctt ctcctgcgaa gccggcgatc aagtacgtaa agagccagca
      241 gaagtgtgaa agcgagaact gggcggcgaa gagggctggc cgaccggccg agtcgccgct
      301 tctctggatc agacggcctg aaggagtcag gaccgcggaa gttccgattc cagcgagtgc
      361 ccagcaggcc atcagtgtcg tcagcgatcc aactgcgaca aacaggaaca tcgaagccgc
      421 caggatcagg ccaccaagga gcatcagcga ccgatcggtg aagcgacgaa gcagagtcgg
      481 caggagcagg gcaataacga tcgatcccgc accaaacgcc cccagagccc aggcgacgtc
      541 ggagtcaccg aatccaagct gtcccttgac gtagatcacc gtgttgatga ggaccatggc
      601 gccggcggcc gcagcggccc agtcgaggag aaagagaccc tggagccgtg gggtacgcag
      661 aaactggcga atgccctggg aaacctgaga caactttctg gcggccaggg cgtcatcccg
      721 gacagccggg acagcggtgg tcaacacgag tcccgcggag gtcaggaagg ccagggaatt
      781 cagggtgaac agctccgtgt agccgaccac agccagaagc aacccggcaa tcaaagggct
      841 gacgagagct tccagctcgt aggccaggcg actgagggat agtgcctcgg tataggtgtc
      901 cacgtcggtg aagatgtcgg ggatcaaggc ctggaatgcc ggggtgaacc ccgccgaggc
      961 cgcactgacg atcactatca ggacgtagac ctgccagatc tcaccgacga aagcgatcga
     1021 accgatggcc agcgcccgga ccacgtccat cgtgacaagc aacttctttc gtgagacacc
     1081 ggcggcgaat gcggctattg ccggggaaac gagaacatag gtggcaactt tcagggcgag
     1141 taccactcca agaatctgac ctgcgttgcc tcctcccatg ctgaatgcca gcaggcccag
     1201 agcgaccgtc gatattccgg ttccaagcag cccgacgatc tgcgcggaaa acagacggcg
     1261 atagacactg ttccggagag gaagtgtcag cgcgggcgcc acggaagcgt tcagcccggg
     1321 aggctgaccc agtcccggga gaggcggcgg gccatagctt cggcaaggcg gatgccggac
     1381 tcgacggtgt cgaagtcgac gttctctgaa acgtcattcg gccagtggta attggccggc
     1441 tgcttgtgct cggtacacga acagatcgag accaccgggt aaccggccgc tagtgcctcc
     1501 agaccatcgg tcccgttgtg gatcctcagg ttcggcatca actcgatgcc ttcctcttcg
     1561 gcggcccgat cggccaggat cagggcttca ggcgggtact cgaacatttt caggaaaccc
     1621 tccccgcgca gcaccgtcaa gtacggggag ccgatcgtgt ccatgttgat gaaaaaggtt
     1681 gagtcgaccg gcaggttcgg aaagtgtcgt tccccgaagg ctttcattcc ctcgctgaat
     1741 gactcctcgg agcccgccga gagaaggatc aggcggagac cctcgggagg gtctgcgaga
     1801 aaccggcggg cgagttcgag cagggcgatc acggccgtgc cgttgtcgtt ggcccccgga
     1861 acgaccttcc tcaggccgat gtcgaccatc gaggcggtcg atccggccga aagcaggagc
     1921 ccggccgctg ccagcttccg gctcccggtc agaccgccga tcgcgctgag cagcgggcca
     1981 ccgatcaccg gggccatcag catcggtgaa gtgtcgaggt gctggaaggc gccggtcttc
     2041 gagactagat cgggaatgcc cgggtggaaa accaggccgg cgttggccgc gtcatggtgg
     2101 gagacgaaaa cgacggtgcg ttccgcttcg gggtcgccca gttcacagac cacgttgaag
     2161 gtgtcgcgct tcggcaggaa ccttctgagg tagcggggcc ggggcgggaa gtcggtggcg
     2221 atcgccgcgg caccggccgc cccgagcagg gcaccggcga ggcgcctgcc acgggtcgcc
     2281 gcaatgccgc ccgcggctcc gagcgccgtc ccgataccga tcggccacca gtaggttccg
     2341 tgggccttct cggtctcgat ccgggcttcc gctccgagtt cagagaaccg gctgaccagc
     2401 cactcggcgg cagcgcgctc ccccgccgaa gccgaaggac gatcgattcg ctcgagctcc
     2461 tcgagtcggg cacggagcca gttctggtcc gcttcgccgt gcggcgcggc aagtttcatc
     2521 ggtgtcccgt ttgccatgga ggagcaagat agccgttgct tggcccgccc cgttcacgcg
     2581 ggtccatacg atgtgggggt gttcggtggc gcaaactcga taactctctt ccgggtagcc
     2641 ggaatccgga tcgcggtcga ctggtcgtgg ttcatcttcc ttttcatcgt gatcgtctgg
     2701 ctctcgggct tctacggcga catcctggac gagcccggga gcacccagtc ctacggactg
     2761 gccctggcca gcgcgatcgg tttcttcggc tcgatcctgc tccacgaact cggtcacgca
     2821 ttcgaggcga agcgcagcgg gatcggcatc cggacgatcc gcctctggct gttcggcggg
     2881 gtcgcagaaa tggaccagga gtccgagtcg ccgggaacgg aattcagagt ggcgatcgcc
     2941 ggaccggtgg tgaccttgct gatcgccgtg atcctctgcg tgatcgggat tcaggcggcc
     3001 gggttcgaca acttccgtga cgccctcgag atgcggatca ccgcggagac caacggcccg
     3061 atcaccatgg tcgcctggct ggcagcggtc aacatcctcg ttctcggttt caacctgctc
     3121 cccgccttcc caatggacgg tgggcggatc gtccgctcga tcgcctggaa gatcaccggc
     3181 aagcgcagcg ccgcgacccg gttcgctgcc ggtcttggtc gcatcttcgg gttcatcttc
     3241 atcgggctgg gcctgctctg gatcttcaac ggaaacctgt tcagcggcct ctggctggcg
     3301 atgatcggtt tcctcgtcaa cagctcggcc cgggcggcca gccgcagcac ggtcggagac
     3361 aagctcgaag g
//
LOCUS       DAOQMF010000101         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15194_length_3358_cov_3.036633, whole genome shotgun sequence.
ACCESSION   DAOQMF010000101 DAOQMF010000000
VERSION     DAOQMF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15194_length_3358_cov_3.036633"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..780)
                     /gene="heR"
                     /locus_tag="PLV77_02530"
     CDS             complement(37..780)
                     /gene="heR"
                     /locus_tag="PLV77_02530"
                     /inference="COORDINATES: protein motif:HMM:NF037099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HNC14756.1"
                     /translation="MPKLRRLNVFAAIFFAAQVAVLLVVAEPVTLPVTGQFLTGPPGG
                     GAYGSTELFRLRIDLLVALFLGLAAFDHFVVATWLRGFYEKNIGRGRNPARWYEYSVS
                     ASVMIVLIAMLSGVTDVVALLAIFGVNASMILFGLAMERANAGRTEVDWSPFIYGCVA
                     GAVPWIAIVIQFILSGSNSDGVPGFVYGIFVTLFLLFNCFALNMWLTYRARGRWRDPL
                     FAEKVYIWLSITAKTALAWQVYSGALAGS"
     gene            complement(810..3146)
                     /locus_tag="PLV77_02535"
     CDS             complement(810..3146)
                     /locus_tag="PLV77_02535"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNC14757.1"
                     /translation="MTGLVNPFVGTEASAPDFGTGGGAGNTFPGATMPFGMVAFGPDT
                     IPSLTNFTAGYTYSDDQIRGFSLTHFSGAGCALLQDVPILPVARSLSGSPVVAGSSDI
                     DPSIVPTFSHRHEEARPGFYSVVLNPGKADQIRSELTATTRTAMGRFTFSKANTGTLT
                     LNAGGSTMANYLAAVKVDPKAREVTGTSESGRFCWEPSKYKVYFAARFDRPFKGYGTW
                     KGAALSPGSKQASDESPDAFNYKPVAGGPPFIPGNPSTTAQAGAFVSFNTSGSRPVGM
                     RVGISFVSVAQARKNLSESAGLTFGEVRDRAGQIWRQEMGKVRVSGGRKEDRRTFATA
                     LYHSLLEPSVQSDVNGLYRGQDLKVHRVAPGHAHYSDISGWDVYRSQIQLLAMIEPRR
                     AADIAASLLRMEKQGGCLPRWPYATQNSNVMNGDPSSPIIATIHALGVRGFDARDALR
                     AMVKGADHPCHSDNADYTEREALDDYLDLGWISQERNVTSGQHSVSERSSPWGTASTT
                     MEYALADFTVSRLARALGRDQLAARFLKRSGKWRNLVNPASQRIEPRFADGSFLPGAN
                     ASTEDGFVEGSSAQYGWFVPQNLAGRFATLGGRKAALQKLDHFFTELNAGPGSPYAFL
                     GNEPTLQTPWIYNWLGRPDKAQSIVRRAQIGLYGAGPGGLPGNDDGGTMSAWFVLSAL
                     GLSPVIPGTDVMPLGSPLFPRASLNLQGHRLQVRAAGADRKRPYVKSLRISGGKWAKP
                     WIHVRRLLRGGRMNWKLSSSPSAWGRGKTLAPPSFGDR"
BASE COUNT          592 a         1077 c         1152 g          537 t
ORIGIN      
        1 ggaaggactc cgcgaaaagt tctggccggg ctggtctcag gaaccggcca gagctccgct
       61 gtagacctgc caggcgagcg ccgtcttggc ggtgatgctc agccagatgt agaccttctc
      121 ggcgaacagg gggtcacgcc acctgccccg ggcacggtag gtcaaccaca tgttgagcgc
      181 gaagcagttg aacagaagaa acagggtgac gaagatcccg tagacgaaac ccggcacccc
      241 gtccgaattg gacccggaga ggatgaactg gatcacgatc gcgatccagg gcacggcgcc
      301 tgccacgcag ccatagatga atggtgacca atcaacttcg gtccgtcccg cattcgccct
      361 ttccatggcc aggccgaaaa ggatcatcga ggcgttgacg ccaaagatcg ccagcagggc
      421 tacgacatcg gtaacgccgg agagcatcgc gatcaggacg atcatcaccg aggcgcttac
      481 cgagtattcg taccagcggg ccgggtttct ccccctgccg atgttcttct cgtagaagcc
      541 gcgcagccag gtggcgacca cgaaatggtc gaaggcggcg aggccgagaa agagcgcgac
      601 cagcagatcg atccggagcc tgaacagttc ggtgctcccg taagcgccgc ctccgggagg
      661 cccggtcaaa aactggccgg tcacgggcag ggtgaccggt tctgcgacta ccagaagaac
      721 cgcgacctgc gccgcgaaaa agattgcggc aaagacattg agtctcctga gcttcggcat
      781 ggccgaaccc tacaaacggg cgcccccggt cagcggtcgc cgaaggaggg cggagccagg
      841 gtcttgcccc ggccccaggc cgacgggctg gaggaaagct tccagttcat gcggccgccc
      901 cggagcaatc ggcggacgtg aatccacggc ttcgcccact tgccgccgct gatcctgagg
      961 gacttcacgt acggccgctt gcggtctgcc cccgccgccc tgacctgcag tcggtggccc
     1021 tggaggttca ggctggcccg gggaaagagc gggctgccga gcggcatcac gtcggtgccc
     1081 gggatcaccg gtgaaagtcc gagggcggaa aggacgaacc aggcactcat cgtcccgccg
     1141 tcgtcgttgc cgggcaggcc gcccgggccc gccccgtaaa gcccgatctg ggcccggcgc
     1201 acgatcgatt gcgccttgtc gggccggccg agccagttgt agatccacgg tgtctgcagg
     1261 gtcggctcat tgccgaggaa ggcgtagggc gatcccggcc cggcgttcag ctccgtgaag
     1321 aagtgatcga gcttctgcag ggcagccttg cggccaccga gggttgcgaa ccggccggcc
     1381 aggttctggg gcacgaacca gccgtactgg gcggagcttc cttcgacgaa tccgtcctcg
     1441 gtcgaggcgt tggcgccggg caggaagctg ccgtcggcga agcgtggctc gatcctttga
     1501 ctggccgggt tgaccaggtt gcgccacttg ccggaacgct tgaggaacct tgctgccagc
     1561 tggtcgcggc cgagtgccct ggccagccgc gagaccgtga agtcggccag ggcgtactcc
     1621 atcgtggtcg aggcggtgcc ccagggggag gatcgctcgg agaccgagtg ctggccgctg
     1681 gtcacgttgc gctcctggga gatccagccg aggtcgaggt aatcgtccag cgcctcccgc
     1741 tcggtgtagt cggcgttgtc ggagtggcag ggatggtcgg ctcccttgac catcgccctc
     1801 agcgcgtccc gggcgtcaaa accccggacc ccgagggcgt ggatcgtcgc gatgatcggc
     1861 gaggaagggt ccccgttcat cacgtttgag ttctgggtcg cgtagggcca gcgcggcagg
     1921 cagccgccct gcttctccat ccggagcagt gacgccgcga tgtccgcggc ccgccggggc
     1981 tcgatcatcg cgagcagctg gatctggctg cggtagacat cccagccgga aatgtccgag
     2041 taatgggcgt gacccggggc gacccggtgg accttgaggt cctggccgcg gtagaggccg
     2101 ttgacatccg actgcacgct cggttccagc agggagtggt agagcgccgt cgcgaaggtg
     2161 cggcggtctt ccttccggcc cccggaaaca cgcaccttcc ccatctcctg ccgccagatc
     2221 tggccggccc ggtcgcgaac ctcgccgaaa gtgaggccgg cgctttcgct caggttcttc
     2281 cgcgcctggg cgacgctgac gaaggagatc ccgacccgca tcccgaccgg gcgcgaaccc
     2341 gaggtgttga agctgacgaa ggccccggcc tgggcggtgg tgctcgggtt gccgggaatg
     2401 aacggtggcc cgcccgcgac cggcttgtag ttgaaggcat cgggcgattc gtcgctggcc
     2461 tgcttcgacc cgggagaaag ggccgcgccc ttccaggtgc cgtacccctt gaacggacgg
     2521 tcgaacctgg ccgcgaaata gaccttgtat ttcgacggct cccagcagaa ccgaccgctc
     2581 tccgaggttc cggtcacctc acgggctttc ggatcgacct tcaccgcggc gaggtagttg
     2641 gccatcgtgc tgccacccgc gttcagggtc agggtcccag tgttcgcctt cgagaaggtg
     2701 aagcggccca tcgcggtgcg ggtggttgcg gtcagttccg agcggatctg gtcggccttg
     2761 ccggggttga ggaccacgct gtagaaaccg ggccgggcct cttcgtggcg atggctgaag
     2821 gtcgggacga tcgacgggtc gatgtcggag gaaccggcga cgacgggtga accggacagg
     2881 ctcctggcca cgggcaggat cggcacgtcc tgcagcaggg cgcagccggc accgctgaag
     2941 tgggtcagcg agaacccgcg gatctgatcg tccgaatacg tgtatccggc ggtgaagttg
     3001 gtcaggctgg ggatcgtgtc gggaccgaag gcgaccatcc cgaagggcat ggttgcgccc
     3061 gggaatgtgt tgcccgcacc accgccggtg ccgaagtctg gagccgaggc ttccgtgccg
     3121 acgaaggggt tgaccagacc ggtcaactca ccggccctcg ctccccccgg agcacacaaa
     3181 accactgttg ccgcgaccag cgcgacccat cccctgcaat tcacccaatg caaattacac
     3241 gccatacccc ctttcgaggg agcgcccggg tcgccgggaa agttagcctc ggcccgatgg
     3301 ccaagaagaa gaaagccggc cgggtcagtg ccggactgat tctctaccgc gtcggacc
//
LOCUS       DAOQMF010000102         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15206_length_3356_cov_4.178734, whole genome shotgun sequence.
ACCESSION   DAOQMF010000102 DAOQMF010000000
VERSION     DAOQMF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15206_length_3356_cov_4.178734"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLV77_02540"
     CDS             <1..298
                     /locus_tag="PLV77_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC14758.1"
                     /translation="PDIQLVIYRVSQEAIGNAVRHSGASRVDAVLARNGDRFELKVTD
                     DGSGFSFDQATSGLGLGGMRERALLVGGELDVESRTGHGTTIRLSVPVREGNPG"
     gene            295..924
                     /locus_tag="PLV77_02545"
     CDS             295..924
                     /locus_tag="PLV77_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC14759.1"
                     /translation="MRVVIADDHGIVRSGIRLLLERQGDLEVIAEAADGIEARDTVVR
                     ERPDLAILDVKMPGLTGLQATREIKAQTPETNVLILSMHDDERYLFEALKAGASGYVL
                     KSQADTDLLDAIRSVERGEPFLSPGAQQTLIRDLLERGEPAGSDLTPREEEIVKLVAE
                     ANTTREIAGMLHLSEKTVENHRANAMKKLGMRDRVELVRYAIRQGLIEP"
     gene            921..1610
                     /locus_tag="PLV77_02550"
     CDS             921..1610
                     /locus_tag="PLV77_02550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC14760.1"
                     /translation="MSPDQVAERVALGPGVEVLIRPIRPTDKHLLADGFAALSPESKF
                     QRFFAPVVRLSESDLVYLTEIDHHHHEALVAIDPDEGDLIGVTRYIRTSPYRDREAEV
                     AIVISDQWRGKGLGKALLSRLATRAREEGVTHFLAVILAENEGSIDLFENFAPKKTHV
                     AAGDPGQVEVRIDLPWSGEFSDSVLGYALRSAARGSHRFRTWRRMRRRMRRRPGREKP
                     AGKVREFGKTD"
     gene            1630..2109
                     /locus_tag="PLV77_02555"
     CDS             1630..2109
                     /locus_tag="PLV77_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC14761.1"
                     /translation="MLFSTKAEYGVRLMVELGRQPNTGPVSLNAIAEAERLPLSYLEH
                     LVAKLRQAGLVTSTRGAHGGYSLAYPAEEITMVEVVEALEGQIAPMECFHETPEGKVL
                     CSHEADSDRACATKLLWTRVQGGVTKALAGTTLAELVEFSRPAEERSKSKTAVGSAA"
     gene            2143..2907
                     /gene="sufC"
                     /locus_tag="PLV77_02560"
     CDS             2143..2907
                     /gene="sufC"
                     /locus_tag="PLV77_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNC14762.1"
                     /translation="MAELEIKNLHVSVEEKEILNGLDLTVEKGRVHALMGPNGSGKST
                     LANVIMGHPALEVTEGSIVFDGEDITDADPDERAQLGLFMAFQYPVSIPGVAVSKYLR
                     MILNAQREARGEQPLKIKEFAAQAREAMDLANIPQDFSSRYLNDGFSGGEKKRMELLQ
                     LALLKPQIAILDETDSGLDIDALRTVAEGINKFAGPDMGALVITHYQRLLHMVEPDVV
                     HVMYEGRIVKEGGKELVDQLEAEGYGWIKEEVEAAA"
     gene            2907..>3356
                     /locus_tag="PLV77_02565"
     CDS             2907..>3356
                     /locus_tag="PLV77_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC14763.1"
                     /translation="MAADSATPAATPTPVRSVDEIRAQFPILTREVKGKPLTYLDNGA
                     TVQKPLAVIEAMDRYYREHNANVHRGVHTLSEEATAAFEATREKVARFINAPSARNIV
                     YTSGTTASINLVAYSWGRANLGPGDEVLITEMEHHANIVPWQIVQEQL"
BASE COUNT          630 a         1143 c         1075 g          508 t
ORIGIN      
        1 cccggacatc cagctggtca tctaccgggt ctcccaggaa gcgatcggca acgcggtccg
       61 ccacagcggg gccagccggg tcgatgccgt ccttgcccgc aacggggacc gcttcgagct
      121 gaaggtcacc gatgacggca gcggtttcag cttcgaccag gccacctcgg ggctcggcct
      181 cggcggcatg cgtgagcggg cgcttctggt cggcggggaa ctcgatgtcg aatcgcgcac
      241 cggccacggg accacgatcc ggttgtcggt cccggtccgg gaaggaaacc ccggatgagg
      301 gtcgtgatcg ccgacgacca cgggatcgtc cgttccggaa tccggctgct gctcgagcgc
      361 cagggcgatc tcgaggtgat cgccgaagcc gccgacggga tcgaggcccg cgacacggtt
      421 gtccgcgaaa ggcccgacct ggccatcctc gacgtgaaga tgcccggtct gaccggactg
      481 caggcgaccc gcgagatcaa ggcgcagacc ccggagacca atgtcctgat cctctcgatg
      541 cacgatgacg agcgctacct gttcgaggcg ctgaaggccg gcgcctccgg ttacgtgctc
      601 aagtcccagg ccgacaccga cctgcttgac gcgattcggt cggtcgagcg cggcgagccc
      661 ttcctctccc ccggcgccca gcagaccctg attcgcgatc tgctcgaacg gggcgaaccg
      721 gccgggtccg atctcacacc gcgggaggaa gagatcgtga agctcgtggc cgaggccaac
      781 acgacgaggg aaatcgccgg gatgctccac ctttccgaga agaccgttga aaaccaccgg
      841 gccaacgcga tgaagaagct cggcatgcgg gaccgggtcg aactggtccg gtacgcgatc
      901 cggcaggggt tgatcgaacc atgagccccg atcaggttgc cgagcgggtg gcgctcggcc
      961 ccggggtcga ggtgctgatc cggccgatcc gcccgaccga caagcacctg ctggccgacg
     1021 gctttgccgc actcagcccg gaatcgaagt tccagcggtt cttcgcgccg gtcgttcgcc
     1081 tgagtgaatc ggacctcgtg tacctgaccg agatcgacca tcaccaccac gaggcgctgg
     1141 tggcgatcga ccccgacgaa ggagacctga tcggcgtgac ccgctacatc cgcacctccc
     1201 cctaccggga ccgcgaggcc gaggtggcga tcgtgatttc ggaccagtgg cgcggcaaag
     1261 gtctcggcaa ggcgttgctt tcccggctcg ccaccagggc ccgggaggag ggcgtgaccc
     1321 acttcctggc tgtgatcctc gccgaaaacg agggctcgat cgacctcttc gagaacttcg
     1381 ccccgaagaa gacccatgtt gcggctggtg atcccggcca ggtcgaggtc cgcatcgacc
     1441 tcccctggag tggcgaattc tcggattcgg tgctcggcta tgccttgcgc tcggccgccc
     1501 gcggcagcca ccgcttccga acctggcggc gcatgagacg ccggatgagg cgtcggccgg
     1561 gccgggaaaa gcccgctggc aaggtgcgag aattcggcaa aaccgactag tgctctagga
     1621 tttatatcga tgctcttctc gaccaaagcc gagtacggag ttcgactgat ggtcgagctc
     1681 ggtcgccagc ccaacaccgg ccccgtttcg cttaacgcga tcgccgaagc cgagcgcctg
     1741 cccctttcct atctggagca cctggtggcg aagcttcgcc aggccggtct ggtcacttcg
     1801 acccgcggcg cccacggcgg ctacagcctc gcctacccgg ctgaggaaat caccatggtc
     1861 gaggtggtcg aggccctcga gggccagatc gccccgatgg agtgcttcca cgagaccccg
     1921 gaaggcaagg tcctctgctc gcatgaagca gacagcgacc gggcctgcgc gacgaagctg
     1981 ctctggaccc gggtccaggg tggcgtgacc aaggccctgg ccggcaccac gctggccgag
     2041 ctggtcgagt tctcgcgccc ggccgaagag cgcagcaaga gtaagaccgc ggtcggcagc
     2101 gcagcctagc cgccgcccca gacttttcga acggagcaac cagtggcaga acttgaaatc
     2161 aagaaccttc acgtcagcgt cgaggaaaag gaaatcctca atggcctcga cctgaccgtc
     2221 gaaaagggcc gggtccacgc gttgatgggc ccgaacggtt ccggcaagtc gaccctcgcc
     2281 aacgtgatca tgggccaccc cgccctcgag gtcaccgagg gttcgatcgt cttcgacggc
     2341 gaggacatca ccgacgccga cccggacgag cgtgcccagc tcggcctctt catggccttc
     2401 cagtacccgg tttcgattcc cggcgtggcg gtcagcaagt acctgcggat gatcctcaac
     2461 gcccagcgtg aggcccgcgg cgagcagccg ctcaagatca aggagtttgc cgcccaggcc
     2521 cgggaagcga tggatctcgc caacatcccc caggatttct ccagccgtta cctcaatgac
     2581 ggcttctccg gcggcgagaa gaagcgcatg gagctgctcc agctggcgct gctgaagccc
     2641 cagatcgcga tcctcgacga gaccgactcc gggctcgaca ttgacgcgct ccgtaccgtc
     2701 gccgagggca tcaacaagtt cgccggcccc gacatgggcg ccctggtcat cacccactac
     2761 cagcgcctgc tccacatggt cgagccggat gtggttcacg tgatgtacga aggccgcatc
     2821 gtcaaggaag gcggcaagga actcgttgac cagctcgagg ccgagggcta cggctggatc
     2881 aaggaggagg tcgaggcggc cgcctgatgg cagccgactc cgccaccccc gccgccacac
     2941 cgaccccggt ccggtcggtc gacgaaatcc gcgcgcagtt ccccatcctg acccgcgagg
     3001 tcaaggggaa gccgctcacc tatctggaca acggcgccac cgtccaaaag ccgctcgccg
     3061 tgatcgaggc gatggaccgc tactaccgcg agcacaacgc gaacgtgcat cgcggcgtcc
     3121 acacgttgag cgaagaagcg acggccgcgt tcgaggccac gcgtgagaag gtggcccgct
     3181 ttatcaacgc gcccagcgca cgcaacatcg tctacaccag tggcacgacg gcgagcatca
     3241 acctggtcgc ctatagctgg ggccgcgcca acctgggccc gggcgacgaa gtgctcatca
     3301 cggagatgga gcaccacgcc aacatcgtgc cctggcagat cgtgcaggaa cagctt
//
LOCUS       DAOQMF010000103         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15263_length_3349_cov_3.125987, whole genome shotgun sequence.
ACCESSION   DAOQMF010000103 DAOQMF010000000
VERSION     DAOQMF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15263_length_3349_cov_3.125987"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLV77_02570"
     CDS             complement(<1..431)
                     /locus_tag="PLV77_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC14764.1"
                     /translation="MGEFFQTYFDFSIMGDNFNAVLEGFWWTIKLSLIGGAFALAWGL
                     ILSVIRQLPGKALAPVRWLAIAYIDCLRGIPMLLTLLLIYGGLGTLQSTGTIPEWLGI
                     PEFFGWPSSFWYGVMALTLTYGAYMAEVYRAGIESIPRGQM"
     gene            complement(513..1373)
                     /locus_tag="PLV77_02575"
     CDS             complement(513..1373)
                     /locus_tag="PLV77_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC14765.1"
                     /translation="MKKSRLIALLSALMILAAMVVVVGCGSSDDDSSSSDNGSTAASG
                     DFSSELINEGTLTVGTDTPYPPFEFGDAPDYNGFDVNLVDEIANRLGLETKWVDTSFD
                     TIFTDVAQGKFDMVASASTITPDRQKTVNFSDPYYSAQQALLVPEGSDISSVEDLDGK
                     TVGAQNGTTGKDFAENETNASRVQGYPNGPAAIEAVKNGQVEATIIDQPVAQDAIDKG
                     QTGFEVATTIPTGELYGFAFAKDTPELLAAVNKTLAEMKDDGSLDKIYQKYFSINAPE
                     EVVNGTTTNP"
     gene            1509..2477
                     /locus_tag="PLV77_02580"
     CDS             1509..2477
                     /locus_tag="PLV77_02580"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNC14766.1"
                     /translation="MTPYYDLLNKPCGGTFDDIVDSIGHTPLVDLKTLSPKEDVRIFA
                     KLESANPTGSVKDRVARSMMQKAEEEGAIAPGQTILEPTSGNTGISLAMICRRKGYPL
                     KVVMPDNVTAERSQLLEMYGAEIVYSEGAKGSNGAVELALEMAEDPQYYMPYQYGNEA
                     NPNAHYDGTAVEILEELDEVTAFVAGLGTGGTLMGNGRRLKEANPETRIVAAEPMQGE
                     LVQGLRSLDDGFIPPIIDLSLLDRKIMVSNMDAIIWTKKLLSQEGIFAGVSSGAVAAI
                     AVRIANEIDSGNIVFLIPDGGWKYLSSGVYTKSIEELGDIDATNWW"
     gene            2488..2886
                     /locus_tag="PLV77_02585"
     CDS             2488..2886
                     /locus_tag="PLV77_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNC14767.1"
                     /translation="MKVPQSLLDEMVEHSKADAPDECCGLIGGAGDEAITLHRMENTA
                     HSPLRYEMDSKEQLKVYKQILEAGHDVVGIYHSHTRTAAYPSQTDINLASGWPDAVYF
                     IVSLENPDEPYVRGFNIREGQVEDVDIQAV"
     gene            2864..3142
                     /locus_tag="PLV77_02590"
     CDS             2864..3142
                     /locus_tag="PLV77_02590"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNC14768.1"
                     /translation="MSIFRRSDSTRSGKTPEPVKVAWANHQAEAELIAGILREEGIPS
                     LIKRNKGFDVPDFLAAGARDIFVPASAADRAREILDDLKSDEDRLPGS"
     gene            complement(3239..3337)
                     /locus_tag="PLV77_02595"
     CDS             complement(3239..3337)
                     /locus_tag="PLV77_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC14769.1"
                     /translation="MNDEDVRVLDGLETGVSDTDTVMILPAMAGGE"
BASE COUNT          643 a         1072 c         1030 g          604 t
ORIGIN      
        1 tccatctggc cgcgcgggat cgattcgatg ccggcgcggt agacctccgc catgtaggcg
       61 ccgtaggtga gggtcagcgc catgacgccg taccagaacg agctgggcca gccgaagaac
      121 tcggggattc ccagccactc cgggatcgtg ccggttgatt gcagggtgcc gagaccgccg
      181 taaatcaaaa gcagcgtaag cagcatcggg atgccgcgga ggcagtcgat gtaagcgatc
      241 gcgagccacc tgaccggggc cagagccttg cccggcagct ggcgaatgac ggagaggatc
      301 aggccccacg cgagggcgaa ggctccgccg atcagggaaa gcttgatcgt ccaccagaaa
      361 ccttcgagga cagcgttgaa gttgtccccc atgatggaga agtcgaaata tgtctgaaaa
      421 aactcaccca tctgatactc ccccgtcact tgttcgagaa ccggacagaa ggggcgtctc
      481 ggggacgccc cttctgtcga ttcccttcgg aactacgggt tggtcgtggt tccgttcact
      541 acttcttcgg gcgcgttgat gctgaagtac ttctggtaga tcttgtccag agagccatca
      601 tccttcatct ccgcgagcgt cttgttgacc gctgccagca gctccggcgt gtccttcgcg
      661 aaggcgaagc cgtagagctc accggtcggg atggtcgttg ccacctcgaa cccggtctga
      721 cccttgtcga tcgcgtcctg cgcaaccggc tggtcgatga tggtcgcttc gacctggccg
      781 ttcttcacgg cttcgatcgc ggccggaccg ttcgggtaac cctgaacacg gctggcgttg
      841 gtctcgttct cggcgaagtc cttgccggtg gtgccgttct gggcgccgac agtcttgccg
      901 tcgagatcct caaccgaact gatgtccgaa ccctcgggca cgagcagcgc ctgctgggcc
      961 gagtagtacg ggtccgagaa gttcaccgtc ttctgccggt cgggcgtgat cgtggaagcc
     1021 gaagcaacca tgtcgaactt gccctgggca acatcggtga agatcgtgtc gaacgaggtg
     1081 tcgacccact tggtttcgag gccgagacga ttggcgatct cgtcaacgag attcacgtcg
     1141 aagccgttgt agtcaggcgc atcgccgaac tcgaagggcg ggtacggggt gtcggttccg
     1201 acagtcagcg tgccttcgtt gatcagttcg gacgaaaagt cgccactggc agcggtgctg
     1261 ccattgtcac tgctcgagct gtcatcgtcg ctgctaccgc agccaaccac gacaaccatc
     1321 gccgcgagga tcatcagcgc cgaaagcagc gcgatcaatc gggatttctt catgccaggt
     1381 tcctccagga gggaattgtt tgctggacgc gcaacccggc cagtatttca cgatttccgg
     1441 gaagatcttc cgaccggcgt ccaagtgact acactttatg aagtaccccc gtccagcggg
     1501 cccacaagat gactccttac tacgaccttc tcaacaagcc ctgcgggggc accttcgacg
     1561 acatcgtcga ctcgatcggt cacaccccgc tggtcgatct gaagacgctt tcgccgaagg
     1621 aagacgtgcg gatcttcgcc aagctcgagt ccgccaatcc gaccggctcg gtcaaggacc
     1681 gggtcgcccg gtcgatgatg cagaaggccg aggaggaggg cgcgatcgcc cccggccaga
     1741 ccattctgga accgacctcc ggcaacaccg gcatctcgct ggcgatgatc tgccgccgca
     1801 agggctaccc gctcaaggtg gtcatgcccg acaacgtcac cgccgagcgc agccagctgc
     1861 tcgagatgta cggcgccgaa atcgtctact cggaaggcgc caagggatcc aacggcgcgg
     1921 tcgaactggc cctggagatg gccgaggacc cgcagtacta catgccttac cagtacggca
     1981 acgaggccaa tccgaacgcg cattacgacg gcaccgcggt cgagatcctc gaggagctcg
     2041 acgaggtgac cgccttcgtg gccgggctcg gcacgggcgg caccctgatg ggcaacggcc
     2101 gtcgcttgaa ggaagcgaac ccggagacca ggatcgtcgc ggccgagccg atgcagggcg
     2161 agctggtcca gggcctccgc tccctcgatg acggcttcat cccgccgatc atcgaccttt
     2221 ccctgctcga ccggaagatc atggtctcga acatggacgc gatcatctgg acgaagaagc
     2281 tgctttccca ggaaggaatc ttcgccgggg tttcttccgg tgcggtcgcc gcgatcgcgg
     2341 tacggatcgc caacgagatc gactcgggca acatcgtctt cctgatcccc gacggcggct
     2401 ggaagtacct ctcctccggc gtctacacga agtcgatcga ggaactcggc gacatcgacg
     2461 cgaccaactg gtggtaggcc cgctccgatg aaagttcccc agtccctgct cgacgagatg
     2521 gtcgagcaca gcaaggctga tgctccggac gaatgctgcg gcctgatcgg cggcgccggc
     2581 gacgaggcga tcactctcca ccggatggag aacaccgccc actccccgct ccgctacgag
     2641 atggattcga aggagcagct caaggtctac aagcagatcc tcgaagccgg ccacgacgtg
     2701 gtcgggatct accactccca cacccggacc gcggcgtacc cctcgcagac tgacatcaac
     2761 ctcgccagcg gatggcccga cgccgtctac ttcatcgtct cgctggaaaa cccggacgag
     2821 ccgtatgtcc ggggcttcaa catccgcgag gggcaggtcg aggatgtcga tattcaggcg
     2881 gtctgattcc acgcgttcgg gcaagacgcc cgagccggtc aaggtcgcct gggccaacca
     2941 tcaggccgaa gccgaactga tcgcgggaat cctccgggag gagggcatcc cctcgctgat
     3001 caaacgcaac aagggattcg acgttccgga cttcctcgcc gccggcgccc gcgacatctt
     3061 cgtccccgcc tccgccgcgg accgcgccag ggagatcctc gacgacctca agtcagacga
     3121 agaccgcctg cccggttctt gaagattctc gatcggagcc ccgcccatcc attcttgtgt
     3181 gcgagtgggt ggggcgggtt ttactgggag ggcgggtcgg accccgcccg acttgcagtt
     3241 attcgccgcc ggccatggcc gggaggatca tcacggtgtc ggtgtcgctg accccggttt
     3301 cgaggccgtc aagcacccgg acgtcttcgt cgttcaggta gacgttgac
//
LOCUS       DAOQMF010000104         3346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15289_length_3346_cov_3.144333, whole genome shotgun sequence.
ACCESSION   DAOQMF010000104 DAOQMF010000000
VERSION     DAOQMF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15289_length_3346_cov_3.144333"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..690
                     /locus_tag="PLV77_02600"
     CDS             106..690
                     /locus_tag="PLV77_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14770.1"
                     /translation="MAEQEPRRPARRKARGSKRVADEAAGVPSANGNGRPAGQSGAEA
                     MKAGYAKAEVKNQAAREALQPLVEGERPTVVTVGAIFSAVVAVLLWGSTLYALFTGAE
                     AGGREVNVFQWAFFALVISAMAWGMWKAKYWAVLGFQMLLVLLILAGVLGMVTAPTLV
                     QIIGTLILTAGLSVLFWFMVKAMARIQMPDRPGS"
     gene            767..1768
                     /locus_tag="PLV77_02605"
     CDS             767..1768
                     /locus_tag="PLV77_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNC14771.1"
                     /translation="MKNSLAGFRSIIAVLALFLVLGFALAPAASADTYTPQQRKEYFD
                     SSKYANDLAFVAQAGRRWIVKRTQPTIGKVRACRKAHFPIGKADPGKDPAADYTVPVA
                     VPANYQPTAPIVAAPDPGPDGQAVTLLPDNSTLEPTKPVRKITRKACAGMKKLAIAMD
                     MDETAMSSFRYGSPQPNYDTISMYRNEILGSQTALKPMLNLFKLARKRGMATFVITAR
                     YEPISADPLVGALMGSADLCSSSLAWTGACGLDINTFNFTKVTWANLKDAGYTGLTDL
                     YMRPPDSESKGEVKNSERAEISLRRGYRIIAMFGDQNSDLEKGFYERGFKYQSPEGD"
     gene            1788..3158
                     /gene="lpdA"
                     /locus_tag="PLV77_02610"
     CDS             1788..3158
                     /gene="lpdA"
                     /locus_tag="PLV77_02610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNC14772.1"
                     /translation="MADESFDVIVIGAGPGGYVAAIRAAQLGKTTAVVESDKAGGRCL
                     NYACIPAKTMLHTAEVLDEARNSADIGVKVQGAELDWDALGQRRTDVSASLSGGVKGL
                     FDKNKIRFIEGEGSLTADGDVEVGGTVYGAGKVILATGSVAQAIPGVDFSDRVLDTWG
                     AWSLAEQPKKIAVVGAGASGSEIASAYIRYGTEVILVEMLDQILPVEDKDVVRVVERT
                     FKKQGIEISTGTPVENVEAGKDSVRFTYGENSAEVDYLVIAGGRRPDVDALGLDAAGV
                     KLDENGRVAVDGFQKTSNDKVYAIGDLVRGSALAHKAMEEGVVAAESAAGAETHPVDL
                     DLVPGATFCHPQVASVGLTEARAKEQGLDVKTGKLKLGGVGAGTVYDDRDGMVKLVVD
                     KEYGEIVGAHIVGNRACDMIAELVAVMALEGGIQELQRIMHPHPTISEAVLDAARAVD
                     KWATHA"
BASE COUNT          590 a         1084 c         1123 g          549 t
ORIGIN      
        1 gtcgcatctg cgtggtccgg gcaatccacg atgcggccga cccgaccgcg gtcgcggcca
       61 gcctgcgccg ctctttcgcc tcggtcggca tggaagtcct gcccggtggc tgagcaggag
      121 ccgcgacggc cggcccgccg caaggccaga gggtcgaagc gggtcgcgga cgaggcggcc
      181 ggggtgcctt cggccaacgg caacggccgg cccgccgggc aatccggggc cgaggccatg
      241 aaggcgggct atgccaaggc ggaagtcaaa aaccaggcgg cccgcgaggc cctccagccg
      301 ctggtcgagg gcgagcggcc gaccgtggtc acagtcgggg cgatcttctc cgcggtggtc
      361 gcggtgctgc tctggggctc gaccctgtac gcgcttttca ccggggccga agccggaggc
      421 cgtgaagtga acgtctttca gtgggccttt ttcgcgctgg tcatctcggc catggcgtgg
      481 gggatgtgga aggcgaagta ctgggcggtg ctcggcttcc agatgctgct cgtcctgctg
      541 atcctggccg gcgttctcgg catggtcacc gcgccgaccc tggtgcagat catcggcacc
      601 ctcatcctga ccgccggact ctcggtgctc ttctggttca tggtcaaggc gatggcccgg
      661 atccagatgc cagaccggcc cgggtcctga tccggaagcc tctgaccgca gcccgcttca
      721 ccagtcgtaa accgcaacat tcggcttgcc aagtagtttt catggcatga agaattcact
      781 tgccggattc cggtcaatca ttgccgtcct cgccctcttc ctcgtgctcg gcttcgccct
      841 ggcccccgct gcctcggcgg acacctacac gccgcagcag cgcaaggagt atttcgactc
      901 cagcaagtac gcgaatgacc tcgccttcgt ggcccaggcc ggtcgccgct ggatagtgaa
      961 acggacccaa ccgacgatcg gcaaggtgcg cgcctgccgg aaggctcatt tcccgatcgg
     1021 gaaggcggac ccggggaagg atccggctgc cgattacacc gtgccggtcg cggttccggc
     1081 gaactatcag ccgactgccc ccatcgtcgc tgcgccggac cccggtccgg acgggcaggc
     1141 ggtgacgctg ttgccggaca attccaccct cgaaccgacc aagccggtga gaaaaatcac
     1201 ccgcaaggcc tgcgccggga tgaagaaact ggcgatcgcg atggacatgg acgagaccgc
     1261 gatgtccagc ttccggtacg gctcgccgca gccgaactac gacacgatct cgatgtaccg
     1321 caacgagatc ctcggctccc agacggctct gaagccgatg ctcaacctgt tcaaactggc
     1381 ccggaagcgc ggcatggcga ccttcgtgat caccgcccgc tacgagccga tttcggccga
     1441 tccgctggtc ggtgcgctga tgggttcggc cgacctctgc agcagctcac tggcctggac
     1501 cggcgcctgc ggcctggaca tcaacacctt caacttcacc aaggtgacct gggcgaacct
     1561 gaaggacgcg ggctacaccg gcctgaccga cctctacatg cgcccgccgg attcggaaag
     1621 caagggcgag gtcaagaact cggaacgggc cgaaatctcg ctgcgtcgcg gttaccggat
     1681 catcgccatg ttcggcgacc agaacagtga cctggagaag ggattctacg agcgcggctt
     1741 caagtaccag agccccgaag gcgactagaa gatagtctcg cctgaccatg gctgatgaat
     1801 cgtttgacgt aatcgttatc ggtgcgggac cgggcggcta tgtggccgcg atccgcgccg
     1861 cccagctcgg caagaccacc gcggtggtcg aatcggacaa ggccggcgga cgctgtctga
     1921 actacgcgtg catcccggcc aagacgatgc tccacaccgc cgaggttctc gatgaagcca
     1981 ggaactcggc ggacatcggg gtcaaggtgc agggagccga actcgactgg gacgcgctcg
     2041 gccagcgccg gaccgatgtc tcggccagcc tgtccggcgg cgtcaaggga ctcttcgaca
     2101 agaacaagat caggttcatc gaaggcgaag gcagcctgac cgccgacggt gacgtcgagg
     2161 tcggtggcac cgtgtacggg gccggcaagg tgatcctcgc caccggttcg gtagcgcagg
     2221 cgattcccgg ggtcgatttc tccgaccggg tgctggacac ctggggtgcc tggtctctgg
     2281 ccgaacagcc gaagaagatc gccgtggtcg gcgccggtgc ctccggctcc gagatcgctt
     2341 ccgcctacat ccgctacggg accgaagtga tcctggtcga gatgctcgat cagatcctgc
     2401 cggtcgagga caaggatgtg gtccgggtgg tcgaacgcac cttcaagaag cagggcatcg
     2461 agatctccac cggtacgccg gtcgagaacg ttgaggccgg caaggactcg gtcaggttca
     2521 cctacggcga gaactcggcc gaggtcgatt acctggtgat cgccgggggc cgccggccgg
     2581 acgtggacgc gcttggtctt gacgcggccg gggtgaagct ggacgagaac ggacgggtcg
     2641 ccgtcgacgg gttccagaag acttccaacg acaaggtcta cgcgatcggt gacctggtcc
     2701 gcgggtcggc cctcgcccac aaggcgatgg aggagggcgt ggtcgcggcc gagtccgccg
     2761 ccggcgccga aacccatccg gtcgatctcg acctggtccc gggcgccacc ttctgccacc
     2821 cgcaggtcgc cagcgtcggg ctgaccgagg cccgggcaaa ggagcagggc ctcgatgtga
     2881 agaccggcaa gctcaagctc ggcggagtcg gggccggcac cgtctacgac gaccgcgacg
     2941 gcatggtcaa gctggtcgtg gacaaggagt acggcgagat cgtcggggcc cacatagtcg
     3001 gcaaccgggc ctgcgacatg atcgccgagc tggtcgccgt gatggccctc gaaggcggca
     3061 tccaggagct ccagcgcatc atgcaccccc acccaacgat ctccgaagcc gtcctcgacg
     3121 ccgccagggc cgtagacaag tgggccaccc acgcttgagc aggagtcggg cggggtccga
     3181 cccgccctcc atatggcacc gccccgccca ctcgaatcat ctgatggttg ggtggggtcc
     3241 ggtttgagaa aggttcgggc ccttgatgga cgagttcttt tttgatctcg ggtcgcctta
     3301 tgcgtggttg agtgcggagc ggattgactc gctgttcgag tcggcg
//
LOCUS       DAOQMF010000105         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15370_length_3336_cov_3.703749, whole genome shotgun sequence.
ACCESSION   DAOQMF010000105 DAOQMF010000000
VERSION     DAOQMF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15370_length_3336_cov_3.703749"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLV77_02615"
     CDS             complement(<1..227)
                     /locus_tag="PLV77_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNC14773.1"
                     /translation="MLPTFVIALREGVEASLIVGIVAAFLVQQGRRDMLRHVLVGVGI
                     AVLICTGIGIGLQLLNEELPQRQQEMLETVIG"
     gene            complement(324..989)
                     /locus_tag="PLV77_02620"
     CDS             complement(324..989)
                     /locus_tag="PLV77_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNC14774.1"
                     /translation="MTDTEETLHYCAYLSYSWDVEAGRGLAPAVEPLVAEIEAAGVEI
                     RGTYATTAFRAECDILFWLLADSTLKLDAAARAIRTSGLAPALTPYWNAIGVHQEAEF
                     NRAHVPAFVAGEPAGDFICLYPFVRSLEWYLLEDEERRELLAEHGMLARDYKDVRANT
                     VSTFGLSDYEWLLAFEASEPVRIVQLMRELRAARARLHTREELPFINGIRRPLAEIVK
                     GLE"
     gene            complement(982..2019)
                     /locus_tag="PLV77_02625"
     CDS             complement(982..2019)
                     /locus_tag="PLV77_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC14775.1"
                     /translation="MKRAVVVGAGIAGLTAAWQLSRSGHEVTVLDRTGRTGGRILPAE
                     FSGRLVDTGPDALGPDPALDELLEQIGMAGQVEIPDPARAFRLTAAGELEPYGVGGGA
                     MFGLFGGIFSLPARLEELLREAGQKFRLGVTVDSVRQEDRSVLVEAEGSKEAFDLAVV
                     ATGARAAGRLLDGETGERLAAIRGVSVSLVHLAFPEKAIGREMNGTGYLADPAEGRLV
                     TGCSWSSAKWKRLAGDPAILRASIREAGQGEVIGLPDREVVEKVMAELVPVMEIEGDP
                     VETMVTRHPEALVVRDEEHESAVAAARGLLAATPRIGLVGADLDGPGISRSIAGVRET
                     VRAIVEGTRND"
     gene            complement(2016..2945)
                     /gene="hemH"
                     /locus_tag="PLV77_02630"
     CDS             complement(2016..2945)
                     /gene="hemH"
                     /locus_tag="PLV77_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNC14776.1"
                     /translation="MSDETAKTGVLVMAYGTATGPDDVEAYYTHIRHGRPPEPEALKE
                     LQERYEMIGGSPLLGITQAQAEGIAAALERSNPGEFTTAMGQKHSAPFIEDAVRELLD
                     RGVERIVGIVLAPHFSTMSIAQYRSRAEEAAAEAGVPVTVIESWHEEPGYLEFLNQAI
                     TDAYEALPAEARESVELAVTAHSLPVAILERGDIYAEELERTRENLAGRPEFTGTTLA
                     WQSAGRTDAEWIGPDICEIMPGLKEAGRTAVVVCAAGFVSDHLEVKFDIDYEAKQAAE
                     EAGLALTRTAMPNDDPGFCATLAGIVAREAGKS"
     gene            complement(2942..>3336)
                     /locus_tag="PLV77_02635"
     CDS             complement(2942..>3336)
                     /locus_tag="PLV77_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNC14777.1"
                     /translation="YREAVLPVSQRLFAEIEDLGVPRIHFAVNAGHLMEAMAEAGSDA
                     MGVDWRTPLSEARRRLGEGVALQGNLDPTVPLAGPEVLERETRNLLADAPERGHVFNL
                     GHGVLPSTDPGMLTELVGLVHEITEKRN"
BASE COUNT          525 a         1188 c         1073 g          550 t
ORIGIN      
        1 ccgatcaccg tttccagcat ctcctgctgg cgctgcggca gttcctcgtt gaggagctgc
       61 aggccgatcc cgatgccggt gcagatgagc accgcgatac ccactccgac cagcacgtgg
      121 cgcagcatgt cacggcggcc ctgctgcacc agaaaggcag ccacgatccc gacgatcaga
      181 gaggcctcga cgccttcacg gagtgcgatg acaaaggttg gcagcatgaa aaccaacccg
      241 tttatacagg attacttcgg tatacctaac tccggctcgg gtcgagccct cggggagagc
      301 ccgcgcggag gtctcgcgac cccttattca agtcctttga cgatttccgc cagaggtctt
      361 cttatcccgt tgatgaaagg cagttcctcg cgggtgtgga ggcgcgccct ggccgctcgc
      421 agctcccgca tgagctggac gatccggacc ggttcactcg cctcgaaggc gagcagccac
      481 tcgtagtcgc tgagcccgaa ggtgctgacc gtgttggccc gcacgtcctt gtaatcgcgg
      541 gccagcatgc cgtgctcggc cagcaactcc cgccgctctt cgtcctcgag caggtaccac
      601 tcgagcgagc gcacgaaggg atagaggcag atgaaatcgc cggccggctc gccggccacg
      661 aaggcgggga cgtgagcccg gttgaactcg gcttcctgat ggacgccgat cgcgttccag
      721 tacggggtca gggcgggagc gagcccggag gtccggatcg cccgggctgc cgcgtcgagc
      781 ttcagggtcg agtcggccag cagccagaag aggatgtcgc attcggcgcg gaacgcggtc
      841 gtcgcgtagg tgccgcggat ctccaccccg gcggcctcga tttcagccac gagcggttcc
      901 accgccgggg ccagcccccg gccggcctcg acatcccagg aataggaaag gtaggcacag
      961 tagtggaggg tttcttcggt atcagtcatt tcgtgtccct tcgacgatcg ccctgacagt
     1021 ctctcgcacc ccggcgatcg agcgcgatat gcccgggccg tcgaggtcgg cgccgaccag
     1081 cccgatccgc ggggtcgcgg ccagaaggcc acgcgccgcc gccaccgccg actcatgttc
     1141 ctcgtcgcgg acgaccaggg cctccgggtg gcgggtgacc atcgtctcga ccggatcgcc
     1201 ttcgatctcc atcaccggaa ccagctccgc catcaccttc tccacaacct cccggtccgg
     1261 cagaccgatc acctcgccct ggccggcttc acggatcgat gcccgcagga tcgccgggtc
     1321 gccggccagc cgcttccact tggccgaaga ccaggaacag ccggtgacca gccggccttc
     1381 ggcgggatcg gcgaggtagc cggtgccgtt catctcgcgg ccgatcgcct tctccgggaa
     1441 ggcaaggtga acgagactga ccgacacccc ccggatcgcg gccagccgtt cgcccgtttc
     1501 cccgtcgagc aggcgcccgg cggcgcgggc accggttgcg accacggcca ggtcaaatgc
     1561 ctccttagat ccttccgctt caacgagcac cgaccggtcc tcttgccgga ccgaatcgac
     1621 cgtaactccg aggcggaact tttgcccggc ttccctcagc aactcctcca gcctggccgg
     1681 cagggaaaag atcccgccga agagaccgaa catcgccccg ccgccgaccc cgtagggctc
     1741 cagctcaccc gccgcggtca gcctgaaggc ccgggccggg tcggggatct cgacctgacc
     1801 ggccatgccg atctgttcga gcagttcgtc cagcgccgga tcgggaccga gagcgtccgg
     1861 gcccgtgtca accagccgcc ccgagaattc agccgggagg atccggcccc cggtccggcc
     1921 ggtccggtcc agcaccgtca cttcgtggcc ggatcggctg agttgccagg ccgcggtcag
     1981 cccggcgatg ccggctccga cgacgaccgc ccgcttcaag acttgccggc ctcccttgcg
     2041 acgatcccgg ccagcgtggc acagaagccc ggatcgtcat tcggcatggc ggtgcgggtc
     2101 agggccagtc ccgcttcttc ggccgcctgc tttgcctcgt aatcgatgtc gaacttgacc
     2161 tcgaggtggt cggaaacgaa cccggcggca cagaccacca ccgccgtccg ccccgcttcc
     2221 ttcaggccgg gcatgatctc gcagatgtcc gggccgatcc actcggcgtc ggtgcgtccg
     2281 gcactctgcc aggccagggt cgtgccggtg aactccggcc tcccggcgag gttctcgcgg
     2341 gtcctctcca gctcctcggc gtagatgtcg cctcgctcga gaatcgccac cggaaggctg
     2401 tgggcggtca ccgccagctc gaccgattca cgggcttccg cgggcagcgc ctcgtaggcg
     2461 tcggtgatcg cctggttcag gaactcgagg tagcccggtt cctcgtgcca cgattcgatc
     2521 acggtgaccg gcaccccggc ttccgccgcg gcttcctcgg cccggctgcg gtactgggcg
     2581 atgctcatgg tcgagaagtg cggagccagg acgatgccga cgatccgctc cactccccgg
     2641 tcgagcagct cccgcacggc atcctcgatg aagggggccg aatgcttctg gcccatggcg
     2701 gtcgtgaact cgcccgggtt cgaccgctcg agggcggcgg cgatgccttc ggcctgggcc
     2761 tgggtgatgc ccaggagcgg tgatccgcca atcatctcgt agcgctcctg aagctccttc
     2821 agggcctcgg gctcaggcgg gcggccgtgg cggatgtgcg tgtaataggc ctcgacgtca
     2881 tccggcccgg tcgcggttcc ataggccatg accaggactc cggtcttcgc tgtttcgtcg
     2941 ctcaattcct cttctccgtg atctcgtgaa caaggccgac cagctcggtc agcatgcccg
     3001 ggtcggtcga aggcaggact ccgtgaccca gattgaagac atgaccccgc tcgggagcgt
     3061 cggccagcag gttgcgggtc tcgcgctcga gcacttccgg cccggccagg ggcacggttg
     3121 gatcgaggtt gccctgcagg gcgacgccct caccgagccg gcggcgagcc tcggagagcg
     3181 gggtccgcca gtccaccccc atcgcgtcgg agccggcctc ggccatcgcc tccatcaggt
     3241 ggccggcgtt gaccgcgaag tgaatccgcg gcacccccag atcctcgatc tcggcgaaga
     3301 gcctttgcga aaccggcagg acggcctccc ggtaag
//
LOCUS       DAOQMF010000106         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15449_length_3325_cov_3.057187, whole genome shotgun sequence.
ACCESSION   DAOQMF010000106 DAOQMF010000000
VERSION     DAOQMF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15449_length_3325_cov_3.057187"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLV77_02640"
     CDS             complement(<1..531)
                     /locus_tag="PLV77_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14778.1"
                     /translation="MFVPSRCPLCRGAIDPDLLVCGECMRELNRSPVLRGEPPEGVDR
                     IVSCADHDGAPRDLLAAFKFRHLTTLGGLIAGYMADAAGQPGEQSLLVPVPPARLRTW
                     FRGFDPVAMLTARVAGLLDIPEPPVPVLARRGSGRQRGRARAGRLSDPPDIRPTEAAG
                     RQVGGRTVLLVDDVMTT"
     gene            complement(608..1624)
                     /locus_tag="PLV77_02645"
     CDS             complement(608..1624)
                     /locus_tag="PLV77_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNC14779.1"
                     /translation="MKAMVLAAGLGTRLRPVTRAVPKPMVPVLNRPVMEHIIRLLARH
                     GFTEVIANIHWYPDVIRDHFGDGSGFGIELSYSFEEELLGTAGGVRNASAFFDDDFLV
                     ISGDAMTDIDLSAMREFHRSHGGMATLATRRVDDTSEFGVVITDDEGRIQGFQEKPDP
                     DEALSDLANCGIYMFNREIFDYFPGPGESKLMAPGHPDDFADWAYDVFPAMLDAGIAF
                     YAHETDAYWNDIGTIEELVQSNFDGLFGDVDLGLPEKQPLPEVWVAGEVEGLDGAEID
                     GPVLIGDGARIGEGAQLVGPLVLGEGCSIGAGAGVREAVLLSRSSLEKGAFLAGGLLG
                     PTRD"
     gene            complement(1624..2610)
                     /locus_tag="PLV77_02650"
     CDS             complement(1624..2610)
                     /locus_tag="PLV77_02650"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNC14780.1"
                     /translation="MRPSMLEDVLDIPNHIRDALWRVDSAQLKARPSAGFVVCGMGGS
                     AIGAELARGVLDNRLTGPMVVSRSYGLPKWVTADWAVLASSYSGDTEETISSFKDAGE
                     AGTHRWVAGTGGELGARARDAGIGVVGLPGFFQPRMTVGYMTVVATYIAHLAGVAPDI
                     RRELEQAADFLDGCKADMEPLARALASQIGDTPVVVHGASLTTAPARRWANQFNENAK
                     QLAFSAEIPEANHNLMEAWSKGSGGLGAVFLTDSGQSARERRRMKLTAESIEGTGAQV
                     FEVETLGETRSERLFWSVMFGDLVSVEVASLRGVDPLPVDEIQNFKRRLGNT"
     gene            complement(2591..>3325)
                     /locus_tag="PLV77_02655"
     CDS             complement(2591..>3325)
                     /locus_tag="PLV77_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNC14781.1"
                     /translation="LGTAGAIRFALDHLDERFFALNGDVLADLDLTALWNQHEETGAQ
                     ATLGLYEVEDSSSYGLVDIDSDGQVLKFSEKDPTRTGTGLINAGTYVLEREAIAAIEP
                     GREVSIETEFYPSITGKGLYARPLEGYWMDFGTRQRYFDASWDILEGRIHTEVEKHSD
                     GVFIAAGAEVDPTATIGPRAVIGAGAKVASGARVTGSVVLEGSEIGADAVIENSIIGP
                     GVNVAARGTVGNEVLGKNEIGETVNA"
BASE COUNT          585 a         1103 c         1071 g          566 t
ORIGIN      
        1 ggttgtcatc acgtcatcga ccagcaggac cgtccggccg ccgacctggc gaccggcagc
       61 ttcggtcggc ctgatgtcgg gcgggtcgga gagccggccg gcgcgggcac ggccgcgctg
      121 gcgaccggaa ccccggcggg ccagcaccgg aaccgggggt tcgggaatgt cgagcaggcc
      181 ggcaacccgt gcggtcagca tcgcgaccgg atcaaagccg cgaaaccagg tccggagcct
      241 ggccggaggc acggggacga ggagcgactg ctcgcccggc tgcccggcgg catcggccat
      301 gtacccggcg atcaatccgc ccagggtcgt gagatgcctg aacttgaagg ccgccagcag
      361 gtccctggga gccccgtcat ggtcggcgca ggagacgatc cggtcaaccc cctccggtgg
      421 ctcaccccgc agcaccggtg agcggttcag ctcgcgcatg cattcgccgc agaccagcaa
      481 atcggggtcg atcgcgcccc ggcagagcgg gcaacgcgaa ggaacgaaca tcccggcaat
      541 ccggtcaagg cccatccccc aacaatcccc gaaaaaacct cacaagtggc tcaacaactg
      601 caacgattca gtcacgagtg ggacccaaca ggccgccggc caggaaggct cccttttcca
      661 gggatgagcg gctgagcagg actgcttccc gcaccccggc ccccgccccg atcgagcaac
      721 cctcgccaag gaccagaggc ccgaccagct gggcgccctc gccgatccgg gccccgtccc
      781 cgatcaggac cggcccgtcg atctcggcgc catcgagtcc ctcgacttcg cccgcgaccc
      841 agacctccgg caggggctgc ttttcgggca gccccaggtc aacgtcgcca aagagtccgt
      901 cgaagttgct ctgcacgagc tcttcgatcg tgccgatgtc attccagtac gcgtcggtct
      961 cgtgcgcgta gaaggcgatc ccggcgtcca gcatcgccgg gaagacgtca taggcccagt
     1021 cggcaaaatc gtcgggatgg cccggggcca ttagcttgct ttcaccggga ccgggaaagt
     1081 agtcgaagat ctcgcggttg aacatgtaga tgccgcagtt cgcgaggtcg gagagagcct
     1141 cgtcggggtc cggcttctcc tggaagccct ggattcgccc ttcatcgtcg gtgatcacga
     1201 cgccaaactc ggaagtgtca tcgacccgtc gggtcgcgag agtcgccatg ccgccgtgtg
     1261 accggtggaa ctcacgcatc gccgaaaggt cgatgtcggt catcgcatca ccggagatga
     1321 ccaggaagtc atcgtcgaag aaggccgagg cgttgcgtac gccgccggcg gtgccgagca
     1381 gttcttcctc gaagctgtaa ctgagttcga tgccgaaacc cgagccgtcg ccgaagtggt
     1441 cccggatcac gtccgggtac cagtggatgt tggcgatgac ttcggtgaag ccgtgacggg
     1501 cgaggagccg gatgatgtgc tccatgaccg gccggttcag gaccgggacc atcggcttgg
     1561 gcactgctcg ggtgactggc ctgagcctcg tgcccagtcc ggcggccagc accattgctt
     1621 tcatcaggta ttgcccaggc gccgtttgaa gttctggatt tcgtcgaccg gcaaaggatc
     1681 gactccgcgc agggaggcaa cctcgaccga gaccaggtca ccgaacatca ccgaccagaa
     1741 caggcgttcc gatcgtgtct cgccaagggt ctccacctcg aagacctggg cgcccgtccc
     1801 ctcgatcgat tcggcggtga gcttcatccg ccgccgttcg cgggccgact ggcccgaatc
     1861 ggtgaggaat acggctccca gccctccgga ccccttcgac cacgcttcca tcaggttgtg
     1921 gtttgcctcg gggatctcgg ccgagaatgc gagctgtttg gcgttttcgt tgaactggtt
     1981 ggcccagcgc ctggccgggg ccgttgtcag gcttgctcca tgcacgacaa cgggagtgtc
     2041 ccctatttgg gacgcaagtg ccctcgccag cggttccatg tccgccttgc agccgtcgag
     2101 gaaatcggcc gcctgctcga gttcgcgccg gatgtcgggg gccacgccgg ccagatgggc
     2161 gatgtaggtg gcgacgacgg tcatgtatcc gaccgtcatc cggggctgga agaagccggg
     2221 caggccgacc acgccgatgc cggcgtcacg ggcccgggcg cccagttcgc cgccggtgcc
     2281 tgcaacccag cgatgggtgc ccgcttcacc ggcgtccttg aacgaggaga tcgtttcttc
     2341 cgtgtcaccc gagtaactcg aagcgagcac ggcccagtcg gcggtcaccc atttgggcag
     2401 accgtaagag cgcgaaacga ccatcgggcc ggtcaggcga ttgtcgagga ccccgcgggc
     2461 aagctcggcg ccaatcgccg atccgcccat cccgcagacc acgaagccgg ctgagggtcg
     2521 agccttgagc tgtgccgaat caacccgcca gagggcatca cgaatgtggt tggggatgtc
     2581 aagtacgtcc tcaagcattg acggtctcac cgatttcatt cttgccaagg acttcatttc
     2641 ctaccgtacc ccttgccgca acatttacac caggtccgat gatcgagttt tcgatcactg
     2701 cgtcggcacc gatttcgctt ccttcgagga cgaccgagcc ggtcacacgg gcaccggaag
     2761 ccactttggc gcctgctcct ataaccgccc tggggccgat agttgcggtt ggatcgacct
     2821 cggcgccggc ggcgatgaag accccgtcgc tgtgcttttc aacctcggtg tggatgcggc
     2881 cttcgaggat gtcccagctg gcgtcgaagt agcgctgcct ggtcccgaag tccatccagt
     2941 agccctccag cggccgggcg tagagaccct tgccggtgat cgaagggtag aactcggtct
     3001 cgatcgagac ctcccggccc ggttcgatcg cggcgatcgc ctcccgctcc aggacatagg
     3061 tgccggcgtt gatcagcccg gtgccggttc gcgtgggatc cttctcgctg aacttcagca
     3121 cctggccgtc ggagtcgatg tcaacgaggc cgtagctgct cgaatcctcg acctcgtaga
     3181 gccccagcgt cgcctgggcg ccggtctctt cgtgctggtt ccagagggcg gtcaggtcga
     3241 gatcggcaag cacgtcgccg ttgagggcga agaaacgctc gtcaaggtgg tcgagagcga
     3301 aacggatcgc ccccgcggtg ccgag
//
LOCUS       DAOQMF010000107         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15459_length_3324_cov_3.645151, whole genome shotgun sequence.
ACCESSION   DAOQMF010000107 DAOQMF010000000
VERSION     DAOQMF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15459_length_3324_cov_3.645151"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLV77_02660"
     CDS             complement(<1..253)
                     /locus_tag="PLV77_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC14782.1"
                     /translation="MADATVAEEEYLQTIFWLMEAGLPITGANIAKAMQLSPPTVHEM
                     IKRLERDGYVTRSADKILHLTEDGRAEAEQITRRHRMIER"
     gene            complement(278..1087)
                     /locus_tag="PLV77_02665"
     CDS             complement(278..1087)
                     /locus_tag="PLV77_02665"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNC14783.1"
                     /translation="MSLRCVYTDLDGTLLGAGASLFTDEEGNFSMAQARALEAASRAG
                     VEVVIMSGRRRAQVHEDARIIGQNSFIFDAGAAFSIDQETTLLLGDFELEEGQTPVQR
                     MSERGVPDLLLENFAGRFEYHDPWHLDREMSHLFRGDIDTAEANALLEENGHGDLRLL
                     DNGAIHRRMEGIEQAHCYHLVPKAVSKVNAVAAHARARGYSPEETIAVGDSLEDLEVA
                     PAVGKFFIVANGPEKDPGVREAMSRYPNAVVTEASNGAGFYEAVVSSLMGA"
     gene            complement(1136..2038)
                     /locus_tag="PLV77_02670"
     CDS             complement(1136..2038)
                     /locus_tag="PLV77_02670"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HNC14784.1"
                     /translation="MLDTFHHGDFAAADISRKLRETVSVVIPARNTAGTIAATVAELQ
                     VLAEDGLVGQILVVDADSADGTAAVAREAGAEVVSENELVEGFGPCRGKGDAMWRSLA
                     AVTGEIVVFIDGDIADFGRHYVTGVLGPLTDPGKHFVKGRYQRPFRFGENEEPAGGGR
                     VTELAARPLLARLAPELLAFDQPLAGEVAARRELLDQVPFACGYGVEIAMLIEVWGRV
                     GIRGMAQVELGTKRNAHQSLAALSGMSSEVIAAMADTLDRLGRKDTGRMKMVEGSGPE
                     VVTRPAFATVPQVSHRSGRKPAAP"
     gene            2046..2993
                     /gene="folP"
                     /locus_tag="PLV77_02675"
     CDS             2046..2993
                     /gene="folP"
                     /locus_tag="PLV77_02675"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNC14785.1"
                     /translation="MASLPGTLRTAAGEINVTEPVIVGVVNATPDSFSDRGDRSTAGS
                     IRQVEQHLEDGAGIIEIGGESNVTNRPAVTPEEEIERILPVVVAAAAAGAIISVDTYK
                     PAVAAAALESGAAIINDISGGGNLELMGVAAEAGAGVVLMHTVVEPKTQRWDEAAYPE
                     GITAHQVRFFEERLESLAEIGIRRDQVVVDPGPDFGKTPAQTVDSLRGLPELCGLGCP
                     VMLAVSRKDFIGAINHRRPSERAPGTFAALAFGLDCGGRLLRVHDVAGTRDFLQVWRA
                     LRGESEVDPALRIADEIRREPPRDAAGSPNPAATSPEDV"
     gene            2986..>3324
                     /locus_tag="PLV77_02680"
     CDS             2986..>3324
                     /locus_tag="PLV77_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC14786.1"
                     /translation="MSESLLSGRWSIAIDPARRDGRIVESASEPAAEARLEPLPASLH
                     PGLAEALNRGGIEQLYSHQAEAFEEAAEGNVILTTATASGKSLAFNLPPLNAIAADPK
                     ARALYLYPTKA"
BASE COUNT          524 a         1160 c         1102 g          538 t
ORIGIN      
        1 agcgttcgat catccggtgg cggcgggtga tctgctccgc ctcggcccgg ccgtcctcgg
       61 tcaggtgaag gatcttgtcg gcgctgcggg tcacgtagcc gtcccgctca agccgcttga
      121 tcatctcgtg gacggtgggc ggtgagagct gcatcgcctt ggcgatgttg gcgccggtga
      181 tcggcaatcc ggcctccatc agccagaaga tcgtctgcag atattcctct tcggcaaccg
      241 ttgcgtcagc catggcttga tcctagacct ttggccttca cgcacccatg agggatgaca
      301 caacggcctc gtagaagccg gcgccgttgc tcgcctcggt gaccaccgca ttcggatagc
      361 ggctcattgc ttcccgcacc ccggggtcct tctccgggcc gttggcgacg atgaagaact
      421 tcccgaccgc cggggcaact tcaaggtcct cgagcgaatc gccgacggcg attgtttcct
      481 ccggggagta accccgggca cgggcatggg cggcgactgc gttcaccttg gaaaccgcct
      541 tcggaaccag gtgatagcag tgggcctgtt cgatgccttc catccggcgg tggatcgcgc
      601 cgttgtcaag cagcctgagg tcaccgtgac cgttctcctc gagcagtgcg ttggcctcgg
      661 ccgtgtcgat atccccgcgg aagagatgtg acatctcccg gtcgaggtgc cagggatcgt
      721 ggtactcgaa gcgtccggca aagttttcga gcagcaaatc ggggaccccg cgctcgctca
      781 tccgctggac cggagtctgg ccctcctcga gctcgaagtc gccgagcagc agggtcgtct
      841 cctggtcgat cgagaaggcc gcgcccgcgt cgaagatgaa cgagttctgg ccgatgatcc
      901 gggcgtcttc atggacctgg gcccggcggc ggccggacat gatcacgacc tccaccccgg
      961 ccctcgaagc ggcctcgagc gcccgggcct gggccatcga gaagttgccc tcctcgtcgg
     1021 tgaagagcga ggcaccggcc ccgagcaggg tgccgtcgag gtctgtgtaa acgcagcgga
     1081 gactcatcgc gcctcacgct atccgctgag gcaccgaagt ggagttcaga caggactacg
     1141 gtgccgcagg tttgcgaccg ctgcggtgcg aaacctgcgg caccgtcgcg aaggcgggtc
     1201 gggtcaccac ttccgggccg cttccttcga ccatcttcat gcgccccgtg tctttccggc
     1261 cgagccggtc cagggtgtcg gccatcgcgg cgatcacctc ggaggacatg ccggaaaggg
     1321 cggccagcga ctggtgcgcg ttccgcttgg tgccgagctc gacctgggcc atgccacgga
     1381 tgccgacccg gccccagact tcgatcagca tcgcgatctc gactccgtag ccgcaggcga
     1441 acgggacctg atcgagcagc tcgcgacggg ccgccacctc gccggccagg ggctggtcga
     1501 aggcgagcag ctccggtgcg agccgggcca gcaggggccg ggcggcgagc tcggtgaccc
     1561 ggccaccgcc ggccggctcc tcgttttcgc cgaaccggaa cgggcgctgg taccggccct
     1621 tcacgaaatg cttgccggga tcggtcagcg ggccgagaac gccggtcacg tagtggcggc
     1681 cgaagtcggc aatgtccccg tcgatgaaga cgacgatctc cccggtcacc gcggcgagcg
     1741 accgccacat cgcgtcaccc ttgccccggc agggcccgaa accctcgacc agttcgtttt
     1801 ccgaaaccac ctcggctccg gcttcccggg cgaccgcggc ggtgccgtcg gcggagtcgg
     1861 catcgaccac gaggatctgc ccgaccagcc cgtcctcggc cagcacctga agctcggcca
     1921 cggttgccgc gatcgtcccc gccgtgttcc gggcagggat caccacactg accgtttccc
     1981 gcagcttgcg gctgatgtcg gcggcggcga agtccccgtg gtggaaggtg tctagcatcg
     2041 tggcgatggc ttctctcccg ggaacactac gcaccgccgc aggcgagatc aacgtgaccg
     2101 agccggtcat cgtcggcgtg gtcaacgcca ccccggactc gttctccgat cgcggtgacc
     2161 gctcgacggc cgggtcgatc cgccaggtcg agcagcacct tgaagacggg gccgggatca
     2221 tcgagatcgg cggcgaatcg aacgtgacca accggcccgc ggtgaccccg gaggaggaga
     2281 tcgaacgaat cctgccggtg gtggttgccg ccgccgcggc gggggcgatc atctcggttg
     2341 acacctacaa gccggcggtc gcggccgctg ccctggaatc gggcgcggcg atcatcaacg
     2401 acatcagcgg cggcggcaac ctggaactga tgggggtcgc ggcggaagcc ggggcgggtg
     2461 tggtcctgat gcacaccgtg gttgagccga aaacccagcg ctgggatgaa gcggcctacc
     2521 cggagggaat caccgcccac caggtccgct tcttcgagga aaggctggaa agcctggccg
     2581 aaatcggcat ccggcgtgat caggtggtgg tcgatccggg ccccgatttc ggcaagaccc
     2641 cggcccagac cgtcgattca ctgcgcggcc tgcccgaact ctgcggcctc ggctgtccgg
     2701 tgatgctggc cgtctcccgc aaggacttca tcggcgcgat caatcatcgt cgcccctccg
     2761 agcgggcccc gggcaccttc gccgccctcg ccttcggcct cgattgcggc ggccgcctgc
     2821 tccgggttca cgacgtggcc ggtacccgcg acttcctcca ggtctggcgg gcgctgcgcg
     2881 gtgaaagcga agttgatccg gccctcagga tcgccgacga aatccgccgc gaaccgcccc
     2941 gagacgcggc cggctccccg aatccggccg ccacctcgcc ggaagatgtc tgagtccctg
     3001 ctttccgggc gctggtcgat cgcgatcgac ccggcccgcc gggatggccg gatcgtcgag
     3061 agcgcctccg aaccggccgc cgaagcccgg ctggaaccgc tgccggcttc gcttcatccc
     3121 ggcctcgccg aagccctgaa ccggggcggc atcgaacagc tttacagcca tcaggccgag
     3181 gccttcgagg aggcggccga gggcaacgtg atcctgacca ccgcgaccgc ttccggcaag
     3241 tcgctcgcct tcaacctgcc tccgctcaac gcgatcgcag ccgaccccaa ggcgcgggcg
     3301 ctctacctct acccgaccaa ggcg
//
LOCUS       DAOQMF010000108         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15525_length_3315_cov_3.832515, whole genome shotgun sequence.
ACCESSION   DAOQMF010000108 DAOQMF010000000
VERSION     DAOQMF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15525_length_3315_cov_3.832515"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(479..1738)
                     /locus_tag="PLV77_02685"
     CDS             complement(479..1738)
                     /locus_tag="PLV77_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC14787.1"
                     /translation="MGKVVNIEESQKRRQSAKRQKRSRTALVLGGGGFTGGVYEIGTL
                     RALDLLAVNSTVNDFDMYIGTSAGSFIGSMLAAGVTPEEMMRVLTDEKGALLERISQD
                     TVLRPNFSEYAERIVTLPLHVAGMVKSMVPRFRDLSVIDIAYGLAEHLPSGMYSNAGI
                     GAYVREALATKGIVDSFDALEHELYLTGTDLDSGERVVFGDKEGEWSGIPVSKAVECS
                     TALPLVYEPVEVNGRTLVDGGIYSTTNVDVAVERGAEFIVVVNPLVPYVNDRNVGLPT
                     VSGSRVRRIADMGLPAIANQAFRLLLHQRLAIAVESWKERYPGVDILLFEPDPDDELM
                     FGTSIMDYSRRLDIATHGFETVTAHLAEDYAYYTEVSEKHGIEISATRLRRVVRKVKE
                     QESSEASVWRRIFEQTTGALLRQTGTS"
     gene            1810..3150
                     /locus_tag="PLV77_02690"
     CDS             1810..3150
                     /locus_tag="PLV77_02690"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNC14788.1"
                     /translation="MKHSLPLLAAVLSLALAAPSAAASPDPLPNPDRHFATPFMHRGE
                     KGASGPADASASAGRACQVMRMLARRGNGAVGLRVFNLRSRKNVCALNSGARRSLASN
                     NKLFTISTALDRLGQDQRLQTRVMAGGKTSRNGVLDGNLYLKGGGDPSLGTARFVNRW
                     FGGHGSEIEKLARKVRQAGIRKVTGRLFFDDSVFDQVRGVADSGYATSPWIGPLSGIA
                     FNEGYTSPSLSRFSNDPARLAARTFVRAMRARGVKIRPRFSPRDTPVKLLKRVVAREV
                     SPDMGWMARATLLDSNNFFAETLLKVLGASFHGLGTTANGVKVVRRTMASLKVDVWPI
                     DGSGLTYGNRSTAHDVVKLLFRARSEPWGKALVAGMPTAGVDGTLEDRMRGSAAQGNC
                     HAKTGTLTGVSALSGYCFNSSGRRFAFSILMNDVTNLDTAHRAQDRIAALIARL"
BASE COUNT          575 a         1103 c         1059 g          578 t
ORIGIN      
        1 gcgccatcgc gacccggcca cccggcgcca cggtcagatg cgcggccaga acccggtcgg
       61 gatgctcaaa gggatcggtg agcactacca cccgttcccc ggtaaccgga ttatcgaacg
      121 cctggccaac ccgagtcatg tccagaacct atccgcaacg gaccccacgg cgagagccac
      181 gctgcccgtt cgggtgaagt tcagctcgag atcgcattca tcggcgctat acgccgacaa
      241 accgcatctc gcgaaaagaa ttcaccactt gcctcagcgg gtcgtgccca aggcgctcct
      301 gcgggtggat gccagggcct tcagccgtgc tctgccgggg aggcatcgcc cgggttcgag
      361 tagccgtggg gtcgggcagg cgtctccggg acgctcaaac agagggcggc tgaatgccgc
      421 ccgactgttg tcccggtgat gcctgcccga tcccgcggcg tgatccagga gacccgggct
      481 aggaagtgcc ggtctggcgg aggagagcgc cggtggtctg ctcgaagatg cggcgccaga
      541 ccgaagcctc ggagctttcc tgctccttga ccttgcgaac cacgcggcgc aggcgggtgg
      601 cggagatctc gatgccgtgc ttctcgctga cctcggtgta gtaggcgtaa tcctcggcga
      661 ggtgagcggt cacggtctcg aagccgtggg tggcgatgtc aagacggcgc gagtaatcca
      721 tgatcgaggt gccgaacatc agctcgtcgt ccggatcggg ctcgaagagc aggatgtcaa
      781 ccccggggta gcgttccttc caggattcga ccgcgatcgc cagacgctgg tgaaggagca
      841 gacggaaggc ctggttggcg atcgccggca ggcccatgtc agcgatccgc cggacccggc
      901 tgccggaaac ggtcggcaga ccgacgttgc ggtcgttcac atagggcacc agcggattga
      961 ccacgacgat gaactcggca ccacgctcga ctgccacgtc gacgttggtg gtcgagtaga
     1021 tgccaccgtc gaccagggtg cggccgttta cctcgaccgg ttcgtagacg agcggcaggg
     1081 cggtcgaaca ttcgacagcc ttggaaacgg ggatgccgga ccattcgcct tccttgtcac
     1141 cgaatacgac cctttcacct gaatcgaggt cggtgccggt caggtaaagc tcgtgctcga
     1201 gcgcgtcgaa ggagtcgacg atgcccttgg tcgcgagcgc ctcgcgcaca taggcgccga
     1261 tgcccgcgtt ggagtacatg ccggaaggca ggtgctcggc caggccgtag gcaatgtcga
     1321 tcaccgacag gtcacggaag cgcgggacca tgctcttgac catgccggcg acatgcagcg
     1381 gcagggtgac gatccgctcc gcgtattcgc tgaaattcgg gcgaaggacg gtgtcctggc
     1441 tgatccgttc gagcagggcg cccttttcat cggtcaggac ccgcatcatt tcctccgggg
     1501 tgaccccggc cgcgagcatg ctgccgatga acgagccggc gcttgtgccg atgtacatgt
     1561 cgaagtcatt gaccgtcgaa ttaacggcaa ggagatcaag cgcccggagg gttccgatct
     1621 cgtatacgcc gccggtgaaa ccaccgccac cgaggaccaa cgcagtgcgg gaacgcttct
     1681 gccttttggc gctctggcgc cgtttctggg attcctcgat gtttacgacc ttgcccatga
     1741 gggtccaatt atgacagcaa tcggtgtcac cttccgctac cttcggcccc aagtttgttg
     1801 caatatccga tgaaacactc tcttccgctc ctcgctgcgg tgctttccct ggccctcgca
     1861 gctccttccg cggccgcttc ccccgacccg ctgccgaatc cggaccggca tttcgccaca
     1921 ccgttcatgc accggggtga aaagggcgcg tccggcccgg ccgatgcgag cgccagcgcg
     1981 ggccgggcct gccaggtgat gagaatgctg gccaggcgag gcaatggtgc ggtcggcctg
     2041 cgggtgttca accttcgcag caggaagaac gtctgcgccc tgaactcggg ggcccgccgt
     2101 tcgctggcct cgaacaacaa gctcttcacg atctccaccg ccctcgaccg gctcggccag
     2161 gatcagcggc ttcaaacccg ggtgatggcc ggcggcaaga caagccggaa cggggttctc
     2221 gacggcaacc tctacctgaa gggcggcggc gaccccagcc tcggaaccgc caggttcgtg
     2281 aaccgctggt tcggtggtca cggatcggaa atcgagaagc tggcccggaa ggtccgccag
     2341 gcggggatcc gcaaggtcac cggccggctc ttcttcgacg attcggtctt cgaccaggtc
     2401 cgtggcgtcg ccgattccgg ttatgcgacc agcccctgga tcgggccgct ctccggaatc
     2461 gccttcaacg agggctacac gagcccttcg ctttcccggt tcagcaacga cccggccagg
     2521 ctggcggccc ggaccttcgt tcgggcgatg cgggcccgcg gagtgaagat ccgcccgcga
     2581 ttctcacccc gggacacacc ggtgaagctg ctcaagcggg tagtcgcccg cgaggtttcc
     2641 cccgacatgg gctggatggc ccgggccacc ctgctggact cgaacaactt cttcgcggaa
     2701 accctgctca aggtgctcgg ggcctccttc cacggcctgg gaacgaccgc caacggggtc
     2761 aaggtggtgc gccggaccat ggcttcgctg aaggtggatg tctggccgat cgacggctcg
     2821 ggcctcacct acgggaaccg gtccaccgct cacgacgtgg tcaagctgct cttccgcgcc
     2881 cgcagcgaac cctggggcaa ggccctcgtc gccggcatgc cgaccgccgg ggtggacggc
     2941 accctcgagg accggatgcg cggcagcgct gcccagggca actgccatgc gaagaccggc
     3001 accctgaccg gcgtctcggc cctctccgga tactgcttca acagctccgg ccggcgattc
     3061 gccttctcga tcctgatgaa cgacgtgacc aacctggaca ccgcccaccg ggcccaggac
     3121 cgcatcgccg ccctgatcgc ccggctttag atgacaacag tcgggccggt cttcgccgga
     3181 tgaccccaga aacccgccct actacttact ttctgagcca ttgctcgact tcggcgtagt
     3241 cgccggtggc cacgagatac ctgatcttgc cggtcctgag cgtgaggatg ccgatcttct
     3301 tgtttccttc gaccc
//
LOCUS       DAOQMF010000109         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15552_length_3312_cov_3.415413, whole genome shotgun sequence.
ACCESSION   DAOQMF010000109 DAOQMF010000000
VERSION     DAOQMF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15552_length_3312_cov_3.415413"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PLV77_02695"
     CDS             <1..1069
                     /locus_tag="PLV77_02695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC14789.1"
                     /translation="KSTDEKIDAVREGYESTLSWRVTRPLRSISDAARRLRGRETEIP
                     WAEVMVEEKVEPDWTSWLDTFFGEELERIDAACAETPPEEAWQLFRGLGDDLWAVLLT
                     REYSLYPNIRALLPGAPEPGLQWMWNGATGLELLGQSKNFYARVRELNERHGRAPLDQ
                     ARILDFGCGWGRLTRFFARDAMPDRLFGCDPTPEILEVCAETGVPGELRQTGFLPERL
                     PFENLDLAFSFSVFTHISEAAHEACLKAIHAALAPGGLAVITVRPPAYLDHDPLMEAA
                     RAELSDDFASREMKEPRFIFVPHPVEDGHPQYDEGEMTYGDTVISLPYIEENWTGLFE
                     IVETGFLTGDMYQVPIALKKR"
     gene            complement(1090..1983)
                     /locus_tag="PLV77_02700"
     CDS             complement(1090..1983)
                     /locus_tag="PLV77_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14790.1"
                     /translation="MAEQTATAAASPEEAMVSSPDSPELREVTGPTALGGGWRRSLDL
                     LYLISINDFKKTYFDTVLGYVWSLFRPLILFAVLLFVFTQIFRIGDDVPHYPVMLLFN
                     IVVFTFFQEATIAAVTSVVAQEGVVRKTQFPRVVIPLAVVLTALFNFGVNLVVVLVFL
                     LASGVPVEWTWLFFPLLVLVLTVITTAVCLLVSSLFVRFRDVGIIWGVLATALFYATP
                     VLYPIEVVPERFHDIVLINPLTPIFIELRHLVIDNSAPTAVSAAGGWLGLLPAVAVTI
                     ALCLLAVKVFSREAPRIAEEL"
     gene            complement(1976..>3312)
                     /locus_tag="PLV77_02705"
     CDS             complement(1976..>3312)
                     /locus_tag="PLV77_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC14791.1"
                     /translation="GPGDRVPPVTPDSMTTDPLAETKTRDITENPVVISVEGLKKSFK
                     IPLHRVESFKERAVHPLRKTEYRELKALDGIDFEVRQGEFFGIVGRNGSGKSTLLKIL
                     ASIYRADAGKVRMAGRLAPFIELGVGFNHDLTAHDNVIMNGVMMGLSRQEAASRLDRV
                     VEFAELEDFKELKLKNYSSGMLVRLAFSLMIQADADIMLIDEVLAVGDASFQQKCADV
                     FNQMRDAGKTIVLVTHDMAAVESFCDRAMLIEGGHVIEAGEPRDVARAYLKLNFDAET
                     PRQETLGFLEDGEVEDDFIPGVDIQAKLIDAWLDDGAGERVTNVEIGDPLRLNLEIEA
                     LRDLERPLFTFQINDAKHTQVTGFNQTLEVEGPGDVIHEGQKVTISGEIVNRLTPGRY
                     LLTCWVTRNRETSERVVQGIKLFEFVIFGMKRTPGLVEAEASLNAKVAGSNG"
BASE COUNT          607 a         1070 c         1085 g          550 t
ORIGIN      
        1 gaagagcact gacgagaaga tcgacgcggt ccgggagggc tacgagtcaa ccctgagctg
       61 gcgggtgacc cgtccgctcc ggtcgatcag cgatgccgcc cggcggctgc gcggccgcga
      121 aacggagatt ccctgggccg aggtgatggt cgaggagaag gtcgaaccag actggacctc
      181 ctggctcgac accttcttcg gcgaggaact tgaaaggatc gacgctgcct gcgccgagac
      241 cccgccggag gaggcgtggc aactgttccg cgggctcggc gatgacctct gggccgtcct
      301 gctgacccgc gagtacagcc tctacccgaa cataagggcg ctcctacccg gcgccccgga
      361 acccggcctt cagtggatgt ggaacggcgc gaccggcctc gaactgctgg gccagagcaa
      421 gaacttctac gccagggtcc gagagctgaa cgagcggcac ggccgggccc cgctcgacca
      481 ggcccggatc ctcgacttcg gctgtggttg gggccggctg acccgcttct tcgcccggga
      541 cgcaatgccg gaccggctct tcggctgcga ccccaccccc gagatcctcg aagtctgcgc
      601 cgagaccggc gtgcccggcg aactccggca gaccggtttc ctgcccgagc ggcttccgtt
      661 cgagaacctc gacctggcct tcagcttctc ggtcttcacc cacatttccg aagcggctca
      721 cgaggcctgc ctcaaggcga tccacgccgc cctggcgccc ggtggcctcg ccgtgatcac
      781 ggtgcgtccg ccggcctacc tggaccacga cccgctaatg gaagcggccc gggcggagct
      841 ttcggatgat ttcgccagcc gggaaatgaa ggagccgagg ttcatcttcg tgccccaccc
      901 ggtcgaggat ggtcaccctc aatacgacga gggcgagatg acctacggcg acacggtgat
      961 cagcctcccc tacatcgaag agaactggac cggccttttc gagatcgtcg aaaccggatt
     1021 cctcaccggc gacatgtacc aggtcccgat cgccctgaag aagaggtagg ccgggcgaag
     1081 ctcgcagacc tagagttctt cggcgatgcg gggagcttcg cggctgaaga ctttgaccgc
     1141 gagcaggcag agggcgatgg tgacggcgac cgcggggagc aggccgagcc atccgccggc
     1201 ggcgctgacc gcggtcgggg cggagttgtc gatcaccagg tgccgcagct cgatgaagat
     1261 cggggtgagc gggttgatca gcacgatgtc gtggaagcgc tccggcacca cctcgatcgg
     1321 gtagaggacc ggggtcgcat aaaagagcgc cgtggccagc acgccccaga tgatcccgac
     1381 gtcccggaat ctgacgaaaa gcgaggagac cagcaggcag accgcggtcg tgatcaccgt
     1441 gaggacgaga accaggagcg ggaagaacag ccaggtccac tcgaccggaa ccccggaggc
     1501 gagcaggaag accaggacca cgaccaggtt cacgccgaag ttgaacagcg cggtcagcac
     1561 caccgcgagc gggatcacca cccgcgggaa ctgggtcttt cgcaccaccc cttcctgggc
     1621 cacgactgag gtcaccgcgg cgatcgtcgc ctcctggaag aaggtgaaga cgacgatgtt
     1681 gaagagcagc atcaccgggt aatgcggcac gtcatcgccg atccggaaga tctgggtgaa
     1741 gacgaagagc agcaccgcga agaggatcag cggccgaaac agcgaccaga cgtatcccag
     1801 caccgtgtcg aagtaggtct tcttgaagtc gttgatcgag atcaggtaga gcaaatccag
     1861 cgagcggcgc cagccgccac cgagcgcggt ggggccggtc acttcccgca gttcgggtga
     1921 atccggactg gagaccatcg cctcttcggg ggatgccgcc gcagtcgccg tttgctcagc
     1981 cattgctgcc cgccaccttc gcgttgaggc tggcttccgc ctcgaccagt ccgggggtgc
     2041 gcttcatccc gaagatcacg aactcgaaga gcttgatccc ctggaccacg cgctccgaag
     2101 tctcccggtt gcgggtgacc cagcaggtca gcaggtagcg gccgggggtg agccggttga
     2161 cgatctcgcc gctgatcgtc accttctggc cctcgtggat cacgtcgccg gggccctcca
     2221 cctcgagggt ctggttgaag ccggtgacct gggtgtgctt ggcgtcgttg atctggaagg
     2281 tgaaaagcgg ccgttcgagg tcacggaggg cctcgatctc caggttgagc cgcagcggat
     2341 caccgatctc gacgttggtc acccgctcgc cggcgccgtc gtcgagccag gcgtcgatca
     2401 gcttcgcctg gatgtcgacc ccggggatga agtcatcctc gacctcgcca tcctcaagga
     2461 agccgagggt ttcctgccgc ggcgtttcgg cgtcgaagtt cagcttcagg taggcccggg
     2521 ccacatcgcg gggttcgccg gcctcgatca cgtggccgcc ctcgatcaac atcgcccggt
     2581 cgcagaagga ttcgaccgcc gccatgtcat gggtgaccag gacgatcgtc ttgccggcgt
     2641 cgcgcatctg gttgaagaca tcggcgcatt tctgctggaa ggaggcgtca ccgaccgcca
     2701 gcacctcgtc gatcagcatg atgtcggcgt cggcctggat catcagcgaa aaggcgagcc
     2761 ggaccagcat cccggaggag tagttcttga gcttgagctc cttgaaatcc tcgagctcgg
     2821 cgaactcgac cacccggtcg aggcggcttg cggcctcctg ccggctgagg cccatcatca
     2881 cgccgttcat gatcacgttg tcgtgggcgg tgagatcgtg gttgaaaccg accccgagct
     2941 cgatgaaggg ggcgagccgg ccggccatcc ggacctttcc cgcgtcagcc cggtagatcg
     3001 aggcgaggat cttcagcagg gtcgacttgc ccgacccgtt gcgaccgacg atcccgaaga
     3061 actctccctg cctgacttcg aagtcgatcc cgtcgagcgc cttcagctcc cggtactcgg
     3121 tctttctgag cggatggacg gcccgctcct tgaatgactc aacccggtgc agggggattt
     3181 tgaagctctt cttcaggccc tcgaccgaga tcaccaccgg gttttcggtg atgtccctgg
     3241 tcttggtctc ggcgagcgga tccgtggtca tggaatcggg agtcaccgga ggaacccgat
     3301 cacccggtcc at
//
LOCUS       DAOQMF010000110         3297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15658_length_3297_cov_3.182912, whole genome shotgun sequence.
ACCESSION   DAOQMF010000110 DAOQMF010000000
VERSION     DAOQMF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15658_length_3297_cov_3.182912"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLV77_02710"
     CDS             <1..742
                     /locus_tag="PLV77_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC14792.1"
                     /translation="GSIPARIFRDSDRIGINFERGDVVAVKGRVEQFRGQLSAEIEDA
                     KKIDPSEIDPADFLPTAYRDREELEGFLEHLAREVHHPGYRAVVEDLLFTGETAEAFR
                     LAPCTRAGHHAYIGGLSEHTVAVGTLVTETCVLHPKLNSDLLMAAALIHDIGKTGEFT
                     YGAEIGLSERGRNLGHLQIGAELVGGAADRAGLDQAERDALLSTVMCHHGTDPLRLRQ
                     FPSAEAIALYRLNALDAQVKTALEHGLT"
     gene            complement(777..1454)
                     /locus_tag="PLV77_02715"
     CDS             complement(777..1454)
                     /locus_tag="PLV77_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNC14793.1"
                     /translation="MKTEIRHNPGFAIARCTLAPGEQMRAESGAMAATSAGVEIEAKV
                     QGGLMKGLKRSVLGGESLFMTTLTAPQSGGWVDVAANLPGDAMVMSTGEELFLTRGTY
                     LASDASVDIDTKWGGFKNIGGGEGGFLIRATGGGEVVVSCYGAIDRIELGAGEQIVVD
                     SGHLVAFEPSVQYKTRKASSGLMNTLKSGEGFVMEFTGPGVIHTQTRNPDFLVSWLTE
                     VLPFTRA"
     gene            complement(1522..2871)
                     /locus_tag="PLV77_02720"
     CDS             complement(1522..2871)
                     /locus_tag="PLV77_02720"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNC14794.1"
                     /translation="MHDIECPHCGKAFTIDEAGYADILNQVRDEAFEKAIHDRLELAE
                     REKLAAVELARTKAASEHKDAATEKDLEIERLREELKASSELARAKATGELKDESAKK
                     DAEIERLKAELDKADVAGKLALKEALGEVEKERDDLKRSLESKDTEQKLLETSLKEKY
                     EIQIKDRDGEIERLKDMKAKLSTKMIGETLEQHCEIEFNKIRAAAFPNAVFGKDNDAS
                     AGTKGDFIFREADPEGTEFASIMFEMKNELDDGVNKKKNEDFFRKLDDDRKKKGCEYA
                     VLVSLLEPENELYNAGIVDVSHLYPRMYVIRPQFFIPIITLIRNEALKTLKLRSELAQ
                     VRAQNVDITNFEEEMENFKSGFQRNFGIASDQFDKAIEQIDKSIANLGKTKDWLLKSA
                     NQLRLANDKAQDLSIKKLTRGNETMKAKFEEARSPDLEEIKRLEIAESDSGPDSRAA"
     gene            3045..>3297
                     /locus_tag="PLV77_02725"
     CDS             3045..>3297
                     /locus_tag="PLV77_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14795.1"
                     /translation="MRELRPGTVIAVIALFIAIGGTATAASGLINGSKIKPGTVTARQ
                     IKNQTITTSKLAPATVASLRGARGPVGATGPAGENGQPGT"
BASE COUNT          548 a         1082 c         1010 g          657 t
ORIGIN      
        1 cggctcgatt cccgcccgga tcttccgcga ctccgaccgc atcgggatca acttcgaacg
       61 tggtgacgtg gtcgcggtga agggccgggt cgagcagttc cgcgggcagc tctcggccga
      121 aatcgaggac gcgaaaaaga tcgacccgtc ggagatcgac ccggccgact ttctgccaac
      181 cgcctaccgc gaccgggaag aactcgaagg ctttctcgaa cacctcgccc gggaagtcca
      241 tcaccccggc taccgggcgg tggtcgagga cctgttgttc accggcgaaa ccgccgaagc
      301 cttccggctc gccccctgca cccgggccgg ccaccacgcc tacatcggcg ggctttcaga
      361 gcacaccgtc gcggtcggca ccctggtcac cgaaacctgc gtgctccatc cgaaactcaa
      421 ctccgacctg ctgatggccg cggccctcat ccacgacatc ggcaagaccg gcgagttcac
      481 ctacggggcc gagatcggcc tgagcgagcg cggacgcaac ctcggccacc tccagatcgg
      541 ggcggagctg gtcggcggag cagccgaccg ggccggtctc gatcaggccg agcgggacgc
      601 cctgctttcg accgtgatgt gtcaccacgg caccgatccg ctgcggttgc gccagttccc
      661 ctcggccgag gcgatcgccc tctaccggct gaacgcgctc gacgcccagg tcaagaccgc
      721 actcgaacac ggcctgacct gagcgaaaag cgggaacctc cagcggccgg tcgcggctag
      781 gcccgggtga agggcaggac ttcggtcagc cagctgacca ggaagtccgg gttcctggtc
      841 tgggtgtgga tgacgccggg accggtgaac tccatgacga aaccctcgcc gctcttcagg
      901 gtgttcatca ggcccgagga tgccttgcgc gtcttgtact gaacgctcgg ctcgaaggcg
      961 accagatgac cggagtcgac cacgatctgc tcgcccgcgc cgagttcgat ccggtcgatc
     1021 gcgccgtagc aggagacgac cacttcgcca ccgccggtcg cgcggatcag gaagccgccc
     1081 tcgccgccgc cgatgttctt gaagccgccc cacttggtgt cgatgtcaac gctggcgtcc
     1141 gaggccagat aagtaccgcg ggtgaggaag agttcctccc cggttgacat gaccatcgcg
     1201 tcgccgggca ggttcgccgc gacgtcgacc cagcccccgg actgcggcgc ggtcagggtg
     1261 gtcatgaaaa gcgactcgcc cccgagcacc gaacgtttca gccccttcat cagtccgccc
     1321 tgcaccttgg cttcgatctc gactccggcc gaggtggcgg ccatcgcccc cgactcggct
     1381 ctcatctgct cgccgggggc gagcgtgcat cgagcaattg caaaaccggg gttgtgacga
     1441 atctcagttt tcatgcgccc accctagccg tcaccgcgaa ggcaaacgcc ggacaacagt
     1501 tccgggggag ttccggagaa ctcaggcagc ccgggagtcg ggcccggaat cggactcggc
     1561 gatttccagc cgcttgatct cttcgagatc cggcgagcgg gcttcctcga acttggcctt
     1621 catcgtttcg ttgccccgcg tgagtttctt gatcgacagg tcctgggcct tgtcgttggc
     1681 aagacgcaac tggttggcag acttgagcag ccaatccttg gtcttgccga gatttgcaat
     1741 ggacttgtcg atttgttcga tcgccttgtc gaactgatcc gaggcgatcc cgaagtttcg
     1801 ctggaagccg ctcttgaagt tctccatctc ctcctcgaag ttggtgatgt cgacgttctg
     1861 ggcccgcacc tgggccagct ctgatctgag cttcagggtc ttgagggctt cattccggat
     1921 cagcgtgatg atcgggatga agaactgtgg ccggatcaca tacatacggg gatagagatg
     1981 cgaaacgtcg acgatgccgg cgttgtagag ctcgttttcc ggttcaagca gcgagaccag
     2041 gacggcgtac tcgcagccct tcttcttccg gtcgtcatcc agcttcctga agaagtcctc
     2101 gttcttcttc ttgtttacgc cgtcgtcgag ctcgttcttc atctcgaaca tgatcgaggc
     2161 gaactcggtt ccttccgggt cggcctcgcg gaagatgaag tcgcccttgg tgccggcgct
     2221 ggcgtcgttg tccttgccga acacggcatt cgggaaagcg gcggcgcgga tcttgttgaa
     2281 ctcgatctcg cagtgctgct ccagggtctc gccgatcatc ttggtcgaga gcttcgcctt
     2341 catgtccttc aggcgttcga tctcgccgtc gcggtccttg atctggatct cgtacttctc
     2401 cttgagtgaa gtctcgagaa gcttctgctc ggtgtctttg gactcaagcg accgcttgag
     2461 gtcgtcgcgt tccttctcga cttcgcccag ggcttccttg agggcgagct tcccggcgac
     2521 gtccgccttg tccagctctg ccttcaggcg ttcgatctcg gcgtccttct tcgcgctttc
     2581 gtctttgagc tctccggtcg ccttggccct ggccagttcg gaactcgcct tcagctcttc
     2641 ccgcaggcgc tcgatctcca ggtccttctc ggtggcagcg tccttgtgct cgctggccgc
     2701 cttggtccgg gccagctcga ccgccgccag cttctcccgt tcggcgagtt ccagccggtc
     2761 atggatcgcc ttttcgaagg cttcgtcacg aacctggttg aggatgtccg cgtaaccggc
     2821 ctcgtcgatg gtgaaggctt tcccgcaatg cgggcattcg atgtcatgca tttccaaggc
     2881 tcctttcggt ttcgccgacg gcccttcagc ttaggcggtg gtcaggatgc tcagactgag
     2941 tcgccttcgg attgcgggtt ctgacatgct ccacgcttga agaccgccgg cacggccgca
     3001 ttcggctcca gggcaggcag ggaaagaacc agcaaggagc aagaatgagg gaattacgac
     3061 cgggcacggt cattgccgtg atcgcgctgt tcatcgcgat cgggggcacc gcgacggcag
     3121 cgtcgggact gatcaacgga agcaagatca agccgggaac cgtcaccgcc aggcagatca
     3181 agaaccagac gatcaccacc tccaagctcg cgccggcgac ggtcgcttcg ctgcgaggcg
     3241 cccgtggccc ggtcggagca accgggccgg cgggggaaaa cggtcagcca ggcaccc
//
LOCUS       DAOQMF010000111         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15666_length_3296_cov_2.296822, whole genome shotgun sequence.
ACCESSION   DAOQMF010000111 DAOQMF010000000
VERSION     DAOQMF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15666_length_3296_cov_2.296822"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PLV77_02730"
     CDS             <1..791
                     /locus_tag="PLV77_02730"
                     /inference="COORDINATES: protein motif:HMM:NF026213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HNC14796.1"
                     /translation="VEFPDSLAKHWSVRSYYGTVSHNVKSTYTRYLGWYDGNPANLEP
                     LVPVESGKRYVEFMGGADNVVSKARESFEKGEYRWVAEVLGHVVFADPANQEARELQA
                     DAFEQLGYQTESSTWRNAYLMGAQELRHGTPSFPIGGTASEDSIRAMTLPMIFNFFAI
                     KIHGVDAADLELDVNLDITDTGESAVLRLSNGVLSHSMTDRDEDAPVLEMSRELLNKL
                     VGNEITLEEAVEAGEVESGPGLEVFRKFGEHLDEFDLWFPIIEP"
     gene            918..1793
                     /locus_tag="PLV77_02735"
     CDS             918..1793
                     /locus_tag="PLV77_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14797.1"
                     /translation="MPETVSGESSANVSAFETQEPTHRVLLVADEVFRGSDLADELAA
                     HGTDRGSIGVFVISPAMTHSLIDQELGNVDQTVIEASDRLETILAELRKVGISARGEV
                     GDADPMVAIGDGVREFEPDQIMVVTHVDSEREYAEKNLWDRLGTDFHAPVTQLKVPHP
                     AGGGGIPAVESVEHFSAHQRTEEEIQTETRNFPPLKKMDIAGILIGFIGTVALGMVAV
                     AVGTEDGGGNLSGSAAIVLLIAMGAFLINVAHIVGLLFFESVRYSGIWQKFMARLSIG
                     ITTIGLAVSLVLWLA"
     gene            1818..2918
                     /locus_tag="PLV77_02740"
     CDS             1818..2918
                     /locus_tag="PLV77_02740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC14798.1"
                     /translation="MTKRVAVIGAGVVGLAAAWALERRGITCLVFEQGAPGGAQSGGE
                     SRIFRHAHNDPRQFELAVRSRQGWREWEAEFGLDLISPDGSMVIGEAAVARLGKYDGE
                     PGVRIRELGPAQLREVLPILAPYDGPALIDDAAGAIHTRLTIASLITSVGLNLVPERI
                     ESVRPRPEGGVEVVSTFGDRVFDSVVVAAGAGTADLAATAGLKVPSQPQAHMRLTLPL
                     AGGIKAPRLACLQDSSGFFGEAAAYGSPVQGNREYAVGLALSTPVDLAADADPLSGLA
                     DRTADYVAEAMPGLDRGGAVSFQRWVTELPWAADGLAIWQRGEAFFIAGHNLFKLAPA
                     LGSALADCVENGEVGRHFRPEDKLGEAVLEAR"
     gene            complement(2984..>3296)
                     /locus_tag="PLV77_02745"
     CDS             complement(2984..>3296)
                     /locus_tag="PLV77_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC14799.1"
                     /translation="EAGHNRQPVSLDQTPGTSEGDQSSPAEWLGFEDSDFETAENRIM
                     LRAAMDELSGEEQMVIQLRFREELTQSEIAERIGHSQMHVSRMLRRILDRMGDRLPAA
                     A"
BASE COUNT          585 a         1036 c         1094 g          581 t
ORIGIN      
        1 aggtcgagtt cccggattcg ctggccaaac actggtcggt ccgcagctat tacggcacgg
       61 tcagccacaa cgtcaagtcg acctacaccc ggtacctcgg ctggtatgac ggcaacccgg
      121 ccaacctcga gccgctggtt ccggtggaat ccgggaagcg ctacgtcgag ttcatgggcg
      181 gggccgacaa tgtggtcagc aaggcccggg aatcattcga aaagggcgag taccgctggg
      241 tcgcggaagt gctcggacac gtcgtcttcg ccgatcctgc caatcaggag gcccgcgaac
      301 tgcaggcgga cgccttcgag cagcttggct accagaccga atcatcgacc tggcgaaacg
      361 cctatctgat gggtgcccag gaactccggc acggcacccc gagcttcccg atcggtggca
      421 cggccagcga ggactcgatc cgggcgatga cgctgccgat gatcttcaac ttcttcgcga
      481 tcaagattca cggcgtcgac gcggccgacc tcgaactgga cgtgaacctg gacatcaccg
      541 acaccgggga atccgcggtg ctgaggctct cgaacggcgt gctcagccat tcgatgaccg
      601 accgggatga ggatgcgccg gtgctcgaga tgtcccggga gctcctcaac aagctggtcg
      661 gaaacgaaat cacgctcgag gaagcggtcg aagcgggcga agtcgagtcg ggtcccggtc
      721 tcgaggtctt ccgcaagttc ggcgagcatc tggacgagtt cgacctctgg ttcccgatca
      781 tcgagccgta aacggaatcg aaggcgagac gaaaaatccc gccttcgccg ctgctttccc
      841 gaggccgctc agcccggtgc tccgggaaat tccgtaaaaa gcggttccgg ttgcctgaag
      901 ccgggtaggt tgaaaatatg cctgagaccg ttagtggtga atcatccgcg aacgtttcgg
      961 cgttcgaaac ccaggaacca actcaccggg tgctgctggt tgccgatgag gtcttccgcg
     1021 gaagtgacct ggccgacgag ctggcagccc acggaaccga tcggggctcg atcggggtct
     1081 tcgttatctc acccgcgatg acccattccc tgatcgacca ggaactcgga aacgtcgacc
     1141 agacggtgat cgaagcgtcg gaccgccttg agacgatcct ggccgaactg cgcaaggtcg
     1201 gcatttcggc ccgtggcgag gtcggtgacg ccgacccgat ggtggcgatc ggagacggag
     1261 tacgggaatt cgagcccgac cagatcatgg tggtcactca cgtcgacagt gagcgggagt
     1321 atgccgagaa gaatctctgg gaccggctcg ggaccgactt tcatgctccg gtcacccagc
     1381 tgaaggttcc ccacccggcc ggaggaggcg ggatccccgc ggtcgagtcg gttgagcatt
     1441 tctcggccca tcagagaacc gaagaagaga tccagaccga aacccgcaac ttcccgccgc
     1501 tgaagaagat ggacatcgcc gggatcctga tcgggttcat cgggaccgtc gccctcggga
     1561 tggtggcggt cgccgtcggc accgaggacg gcggtggcaa cctctcgggt tccgcggcaa
     1621 tcgtgctgct gattgcgatg ggtgcgttcc tcataaacgt ggctcacatc gtcggactgc
     1681 tgttcttcga aagcgtcaga tatagcggca tctggcagaa attcatggcc aggctctcaa
     1741 tcgggatcac gacgattggc cttgcagtct cactcgttct gtggctcgcc tgaccccgat
     1801 caagcaaaat cagggccatg acgaagcggg tcgcggtaat cggagccggg gtggttggtc
     1861 tggccgccgc ctgggcgctg gagcgccggg gcatcacctg cctcgtcttc gagcagggtg
     1921 ctccgggcgg ggcccagtcc ggtggtgaat cgcggatctt ccgccatgcc cacaacgacc
     1981 cgaggcagtt cgagctcgcg gtcagatcgc gccagggctg gcgtgaatgg gaggccgaat
     2041 tcggcctcga tctgatctcg cccgacggat ccatggtgat cggcgaggcg gcggtcgccc
     2101 ggcttggcaa gtacgacggg gagcccggcg tgcggatccg cgaactcggg ccggctcagc
     2161 tccgggaagt gctgccgatc ctcgcccctt acgacggacc ggccctgatc gacgatgcgg
     2221 ccggggcgat ccacacccgg ctgacgatcg ccagcctgat cacctcggtc gggctcaacc
     2281 tggtcccgga gcggatcgaa tcggtccggc cccgcccgga aggcggggtc gaggtcgtga
     2341 gcaccttcgg cgaccgggtc tttgactccg tcgtggtcgc tgccggcgct ggtacggccg
     2401 acctggctgc gacggccggc ctgaaggttc cttcccagcc gcaggcccac atgcgcttga
     2461 ccctgccgct cgcgggcgga atcaaggctc cccgcctggc ctgccttcag gactcttcgg
     2521 ggttcttcgg cgaggcggcc gcctacggat caccggttca gggaaaccgt gaatacgcgg
     2581 tagggctcgc cctcagcacc ccggttgacc tggccgctga cgctgacccc ctctccgggc
     2641 tggccgatcg caccgcggac tacgtggcgg aagccatgcc gggcctcgac cgcggcgggg
     2701 ccgtttcatt tcagcgatgg gtaaccgagc tgccctgggc agcggacggt ctcgcgatct
     2761 ggcagcgggg cgaggcgttc ttcatcgctg gccacaatct gttcaagctc gctcccgccc
     2821 tgggctccgc ccttgccgat tgtgtggaaa acggcgaggt ggggcggcat ttccggccgg
     2881 aagacaaact cggtgaagcg gtactcgaag ctcgctgatt aaacaaaccg accggccagg
     2941 gggcgggccg gtcggagtgt ttcctttggg ggagtcggga gggtcaggct gcggccggaa
     3001 gccgatcgcc cattcggtcg aggatccgcc gcagcatccg ggacacatgc atctgggagt
     3061 ggccgattcg ctcggcgatc tcggactggg tcagctcctc ccggaagcga agctgaatca
     3121 ccatctgctc ttcgccgctc agttcgtcca tcgcggcccg gagcatgatg cggttctcgg
     3181 ccgtctcgaa atccgaatct tcgaagccaa gccattccgc cggtgaactc tgatcaccct
     3241 ccgaggtgcc gggggtctgg tcgagagaaa ccggttggcg gttgtggccg gcctcg
//
LOCUS       DAOQMF010000112         3269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_15889_length_3269_cov_3.154636, whole genome shotgun sequence.
ACCESSION   DAOQMF010000112 DAOQMF010000000
VERSION     DAOQMF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15889_length_3269_cov_3.154636"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PLV77_02750"
     CDS             <1..607
                     /locus_tag="PLV77_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14800.1"
                     /translation="TEAFASASGNLLDSYFASILTVIALLASGFTVSAVLRPHSEESR
                     GRVGLLLAAPLGKVRWVVNHLLIAFAATAVIIALVGLGLGIGFAVATGDSSQIGRLTG
                     AAVAQTPAMWLTGSLVVLLYGLSSRLSPIAWGLLATFVLIWTLASFGDIPDWIVDLSP
                     FSHTPAVPAVPLDLTPLLVMTALSAALTAGGLALWRRRDLL"
     gene            615..1646
                     /locus_tag="PLV77_02755"
     CDS             615..1646
                     /locus_tag="PLV77_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HNC14801.1"
                     /translation="MTGDSRLRESIRTLYPGYFALVMATGIISNGFYLLDFKVLSAVM
                     IVIAVGAFLVLATATIARALLFPRELAADLVDPRFVFSFFTLVAGVNVLGIQLFMRGK
                     LTPAIILWIFSIVVWVPLSYLSFAVVTFRDSRQGGGVMNGGWLIAIVGAESIAALGGK
                     LSTQIGTGAELVFVIAHAFWGIGMILYGIFATLFANRIFFFRVTPEEMNPLFWVVMGA
                     AAITVNAGSAMIIAQSEIGYLADLEPFVEGASLVLWAWATWWIPLLVIFGIWRHVVRK
                     VPLTYSPMYWSLVFPLGMYTVATWQFATADNFSFMKEIPSYTIWIALTAWLVTATGLV
                     RSIFRRPTT"
     gene            1719..1792
                     /locus_tag="PLV77_02760"
     tRNA            1719..1792
                     /locus_tag="PLV77_02760"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1753..1755,aa:Met,seq:cat)
     gene            complement(1880..2581)
                     /locus_tag="PLV77_02765"
     CDS             complement(1880..2581)
                     /locus_tag="PLV77_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14802.1"
                     /translation="MLGRKIAVTICCLIAYAGALSVQGATADVAVPVNISPANNAVLI
                     ADDPSPWVTLPDVALITLKYSCDASKASSYFVEITGGDSTPGLLGTTLSSYNDGVCEA
                     TNSDGYEPGTYTWRPFLITGLGDAGQAYGSKWTFTVEERPSDPDPDPILDTHMNLSQA
                     KFYARAQTKKRFYRAPFRVSCWRKSSSSFGCFSFYRQNWRKRFRAKWVFAHVVDDNQL
                     WYTTSFKGKSRYSGW"
     gene            complement(2640..>3269)
                     /locus_tag="PLV77_02770"
     CDS             complement(2640..>3269)
                     /locus_tag="PLV77_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14803.1"
                     /translation="RRERLGGPESASAWIEADHQNGLTYAARIVGVGKYAVIAEAKVF
                     PTQPDRVEARTDFLGRSHGERSRRGGEIVDADASPSPGGLTTGDVRKMPTDALLSMAR
                     ARLLKIAEVPSPVLDCFPELSVPRREGERPHRRDDRAVANVAATYAELVESGHRKPLR
                     RTAELHGYAIKGVEKVIAAARERDILTPTTQGKAGGELTNLGRQLAQSQ"
BASE COUNT          520 a         1064 c          976 g          709 t
ORIGIN      
        1 caccgaagca ttcgcatcgg cgagcgggaa cctcctcgat tcctatttcg cctcgatcct
       61 caccgtgatc gccctgctgg cttcgggatt cacggtcagt gccgtgctgc gaccgcattc
      121 ggaagagagt cgcggccggg taggcctcct gctcgccgcc ccgctcggca aggtccgctg
      181 ggtcgtcaac cacctgttga tcgccttcgc ggccaccgcg gtcatcatcg ccctggtcgg
      241 cctgggtctc gggatcggct tcgccgtagc gaccggtgat tcgagccaga tcggccgcct
      301 gaccggagcg gcggtcgcgc agacgcccgc gatgtggctg accggcagcc tcgtcgtgct
      361 gctctacggg ctttcctccc ggctcagccc catcgcctgg ggcctcctcg ccaccttcgt
      421 cctgatctgg accctggcct ctttcgggga catccccgac tggatcgtcg acctctcgcc
      481 cttctcgcac acgccggcgg tcccggcggt gccgctcgac ctcactcccc tcctggtgat
      541 gacggccctg tcggcggcac tcacggctgg cgggctcgca ctctggcgcc gcagggatct
      601 actctgagaa gtcgatgacc ggcgattcaa gactgcgcga atcgatccga accctctacc
      661 cgggctactt cgcgctggtg atggcgaccg gcatcatctc gaacggtttc tacctgctgg
      721 atttcaaggt gctttcggcg gtgatgatcg tcattgccgt aggggccttc ctggtgctgg
      781 ccacggcgac gatcgcgcgg gccctgctct tcccccgcga actcgcggcc gacctggtcg
      841 acccgcggtt cgtcttttcc ttcttcaccc tggtcgccgg ggtgaacgtg ctcggcatcc
      901 agctgttcat gcgcggcaag ctgaccccgg cgatcatcct ctggatcttt tcgatcgtgg
      961 tctgggttcc cttgagctat ctcagcttcg cggtggtgac cttccgcgac tcccggcagg
     1021 gcgggggcgt gatgaacggt ggctggctga tcgcgatagt cggggcggaa tcgatcgccg
     1081 ccctcggcgg caagctcagc acccagatcg ggaccggcgc cgagctcgtc ttcgtgatcg
     1141 cccatgcctt ctggggaatc ggcatgatcc tttacggaat cttcgccacc ctgttcgcca
     1201 accggatctt cttcttccgg gtgaccccgg aggagatgaa cccgctcttc tgggtcgtga
     1261 tgggcgccgc cgcgatcacg gtgaacgccg gttcggcgat gatcatcgcc cagtcggaga
     1321 tcggatatct ggccgatctc gagccgttcg tggagggcgc ttccctggtg ctctgggcct
     1381 gggcgacctg gtggattccc ctgctggtga tcttcgggat ctggcggcat gtcgtgagaa
     1441 aggtgccgct cacctacagc ccgatgtact ggagcctggt cttccctctg ggcatgtaca
     1501 cggtcgccac ctggcagttc gcgacggccg acaatttctc cttcatgaag gagattccgt
     1561 cgtacacgat ctggatcgcc ctgaccgcct ggctggtcac cgcgaccggt ctcgtccgct
     1621 cgatcttccg ccgccccacg acctgatcgc cgccgccccg gggcgaggtc cccgacaggc
     1681 ccagccagcc cctgatcatc tagactcacc ggtcccctgc cggggtagct cagctggtta
     1741 gagcagcgga atcataatcc gcgtgtcggg ggttcgagtc cctcccccgg tactagagct
     1801 ggagccaaat cactctcgca ttcggcccga aaatccgcaa ggtggcaacg aaagtggcaa
     1861 cgttctagtc cggcgatgcc taccagcccg agtagcggct cttacctttg aagcttgttg
     1921 tgtaccagag ctggttgtca tcgactacgt gtgcgaagac ccacttcgca cggaaccttt
     1981 tccgccagtt ctggcggtag aagctgaaac agccgaagct ggaggaggac tttcgccaac
     2041 aactgactcg aaacggtgct cggtagaagc gcttcttcgt ctgcgccctc gcgtagaact
     2101 tcgcttgcga gaggttcatg tgcgtgtcca agatcgggtc tggatcgggg tcgcttggcc
     2161 gttcctccac agtaaaggtc cacttggagc catatgcttg gcccgcgtcc ccgaggccgg
     2221 tgatcagaaa tgggcgccac gtataagttc ctggctcgta cccatctgaa ttggtggctt
     2281 cacacacccc gtcgttatac gaggacagcg tagtgccaag aagccccgga gttgagtccc
     2341 cgcctgttat ctccacgaag taagagctgg ccttgcttgc gtcgcatgaa tatttgagcg
     2401 taatgagcgc aacgtcgggc agcgtgaccc acggactagg gtcatcagcg ataagcacgg
     2461 cgttgttcgc tgggctgatg ttcacgggaa ccgccacgtc tgcggtcgcg ccttggacgc
     2521 taagcgcgcc cgcataggcg atcagacagc aaatcgtgac tgcgatcttc ctccccagca
     2581 tggtcgtcaa atcttatcca aggcttccaa ttatcagatg gggcgaagtc gcatcgtcct
     2641 tactgggatt gcgcgagttg cctgccgagg ttggtcagtt cgccgccggc ttttccctgt
     2701 gtggtcggag tgaggatgtc tcgctcccta gcggcagcga tgaccttttc gactcccttt
     2761 attgcgtagc catgaagttc cgccgttctc ctgagcggct ttctgtgtcc agattccacg
     2821 agttccgcat acgttgcggc gacgtttgcc accgctcgat catctcgtct gtgaggccgt
     2881 tccccttccc gtcttgggac cgatagctcg gggaagcagt caagcacggg tgacggcacc
     2941 tcagcgatct tcaacagtcg tgctctggcc attgagagga gagcgtcggt gggcatcttt
     3001 cgcacgtcgc cagtagtcaa gccccctggt gacggcgagg catccgcatc aacgatctcg
     3061 ccaccccgtc gagatcgctc gccgtgagat cttccgagaa agtcggttct agcctcgacc
     3121 cgatcaggct gagttgggaa gaccttcgct tcggctatga ccgcgtattt accgactccg
     3181 acgatacgag ccgcataggt cagaccgttc tgatggtcag cttcgatcca agccgaagcc
     3241 gactccgggc cgccgagccg ttcccggcg
//
LOCUS       DAOQMF010000113         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16023_length_3251_cov_3.919587, whole genome shotgun sequence.
ACCESSION   DAOQMF010000113 DAOQMF010000000
VERSION     DAOQMF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16023_length_3251_cov_3.919587"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLV77_02775"
     CDS             <1..578
                     /locus_tag="PLV77_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC14804.1"
                     /translation="DEYRRTSIAEQAIEVAAVLRELKVEDATLVGLGFGAVVALEIAL
                     ADPELAARSFLIEPPVFGALKEATEGMSADVDAIRAAAGEGGEEAAYELFLTGRLHTL
                     GVGAERFADHADRGPAAARSFLIEIPAVPAWSLDPVRLAGLESEVTVVTLPSTPDLLG
                     RASQAVADRVPAAATIHSSRDGIAAVAALLA"
     gene            complement(575..1300)
                     /locus_tag="PLV77_02780"
     CDS             complement(575..1300)
                     /locus_tag="PLV77_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14805.1"
                     /translation="MGQYATASLTIDLPHVPLEAGSGPENPPCPACGEPLFPWVGMPV
                     GSGIAHRCEACGLGVLSHGEKFSFPPPVGMEGATDRGKGPDSFDPGTSVDALRELDSE
                     RDEDGSYLFENRASLVCWVTGGAWVGLGTDRRYRFTPAAVTDLIASRDQVVTKVRWRP
                     LRGIAVTWQSGLNMFTFGQNTVLGSLGKVFGVPADRPWKRGLDWFISVAVAIPAIIVA
                     VPLELIGILFRRGASARAEVQVL"
     gene            1299..1472
                     /locus_tag="PLV77_02785"
     CDS             1299..1472
                     /locus_tag="PLV77_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14806.1"
                     /translation="MSSSDSDPGRIESLIVAVSPLLDLVLAAGERISRIAEPVDYEYY
                     PVRDKDEEERSGE"
     gene            1509..2438
                     /locus_tag="PLV77_02790"
     CDS             1509..2438
                     /locus_tag="PLV77_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14807.1"
                     /translation="MNSQSEILEREKRWSLIAGIASVGAVALFFIGQIVGRSGVGSPE
                     GTADFLAALSGHHSTAMLGAVIQGIATVLLAVPLAYLFFAALGRSDRMRRGLIAVVIA
                     GPLFVGIGSIISTSSLLSAADQWKGDQTPAVVKCIDEAQGDSADEVTEEQKDTCRDDA
                     ATDLRNDQSLTNLAVGLSLSGALGFLIALMYTSLNAMRVGLLTRFWGSLGMAVGVLLI
                     FPVLQYVALAWFIFLGFLIVGWLPGGRPEAWSAGVAVPWPVPGEIRQDQDETVIEGSA
                     EEVDPSAGDLEAGEPEGSDEGPQGELRKRKKRN"
     gene            2467..>3251
                     /locus_tag="PLV77_02795"
     CDS             2467..>3251
                     /locus_tag="PLV77_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HNC14808.1"
                     /translation="MASQSLTVAEFIELPIVKAGLPELVAGSEGLDAEVRWVHPLDVP
                     DVSDLLRGGEMILTTGVSIGEDATAQRRFVRDLEAEGAVGIAVELGFAWSRELPKALV
                     DEADRRHIPMVAFRRGIRFVEVSEVVNGSLLDSGHALARRGEELHRSLDRLVLEGEGA
                     ESVLAEVSRRIGNPVVLEDARGELVAVGSATRPESEVVDTWSGLKWSDRNHAEAEGAL
                     AAQVMVRGRNWGRVIALQTDGEFDRFTPIALERAAVAVGLGLM"
BASE COUNT          524 a         1082 c         1112 g          533 t
ORIGIN      
        1 cggatgaata ccggcggacc tcgatcgctg aacaggcgat cgaagtcgct gcggtcctgc
       61 gcgaactgaa ggtcgaggat gccaccctgg tcgggctcgg tttcggcgcg gtcgtggccc
      121 tggagattgc cctcgccgac cccgaactgg cagctcgctc cttcctgatc gagccgcccg
      181 tcttcggcgc cctgaaggaa gcgaccgagg ggatgtcagc agacgtcgac gcgatccggg
      241 cggccgccgg agagggcggg gaggaagccg cctacgaact ctttctgacc ggccggcttc
      301 acaccctcgg ggtcggggcc gaacgcttcg ccgaccacgc cgaccggggt ccggccgcgg
      361 cccgttcatt cttgatcgag attccggcgg ttcccgcctg gtccctggat ccggtccggc
      421 tggccggact cgaatcggag gtgaccgtcg ttaccctgcc gtcaaccccg gacctgctcg
      481 gccgggcctc gcaggctgtc gccgaccgtg tgccggcagc ggcgaccatt cattccagcc
      541 gggacggcat cgccgccgtt gcggccttgc tcgcctagag gacctggact tcggcccggg
      601 ccgaggcgcc gcggcgaaag aggatcccga tcagctcgag cgggacggcg acgatgatcg
      661 ccgggatcgc aaccgcgacc gagatgaacc agtcgagccc gcgcttccag ggccggtcgg
      721 ccggaacgcc gaagaccttg cccagcgagc cgagcacggt gttctggccg aaggtgaaca
      781 tgttcagtcc cgactgccag gtgaccgcga tgccccgcag cggccgccag cgcaccttgg
      841 tcacgacctg gtcgcggctc gcaatcaggt cggtcaccgc agccggagtg aaccggtagc
      901 gacgatcggt cccgagtccg acccacgccc ctccggtaac ccagcaaacc agcgaggccc
      961 ggttctcaaa gaggtaggaa ccatcctcgt cccgttccga atccagctca cgaagcgcgt
     1021 cgaccgaggt tccgggatca aacgaatcgg gccccttgcc acgatccgtg gctccctcca
     1081 ttccgaccgg cggcggaaag ctgaacttct caccgtggct gaggacaccg agcccgcagg
     1141 cctcgcagcg gtgggcgatc ccggagccga ccggcatgcc gacccagggg aagagcggct
     1201 cgccgcaggc cgggcaggga ggattttccg gaccggatcc ggcttcgagg ggaacgtggg
     1261 gcagatcgat cgtcaaactc gctgtagcgt attgccccat gagttcatcc gactccgacc
     1321 ccggcaggat cgaatcgctg atcgtcgccg tatcgccgct gctggatctg gtcctcgcgg
     1381 ccggtgagcg aatctcgcgg atcgccgaac cggtcgatta cgagtactac ccggtccggg
     1441 ataaggacga agaggaacgc tccggggagt gaccggcctg taggcttgcc cggccttcgg
     1501 gcccacccat gaattcacag agcgagatac tcgagcgcga gaagcgctgg tccctgatcg
     1561 cgggcatcgc ttcggtcgga gccgtcgccc tcttcttcat cgggcagatc gtcggccggt
     1621 ccggagtcgg atcgcccgag gggacggccg atttcctggc cgccctgtcc ggtcatcact
     1681 cgaccgcgat gctgggcgcg gtcatccagg gcatcgccac cgtcctgctc gcggtcccgc
     1741 tcgcctacct gttcttcgcg gcgctgggcc gatccgaccg gatgcgccgc ggcttgatcg
     1801 cggtcgtgat cgccggcccc ctcttcgtcg gcatcggctc gatcatttcg acctcttcgc
     1861 tgctcagcgc ggctgaccaa tggaagggcg accagactcc ggccgtcgtc aagtgcatcg
     1921 acgaggctca gggagattcc gccgacgagg tcaccgaaga acagaaggac acctgccgcg
     1981 acgatgcggc gaccgacctg cgcaatgacc agagcctgac caacctcgcg gtcgggcttt
     2041 cactctccgg cgcgctcggc ttcctgatcg cactcatgta cacctcgctc aacgcgatgc
     2101 gggtcggcct cctgaccagg ttctggggct cactcgggat ggcggttggc gtcctgctga
     2161 tcttcccggt gcttcagtac gtggcccttg cctggttcat cttccttggc ttcctgatcg
     2221 tcgggtggtt gcccggcggc aggccggagg cgtggtcggc cggcgtcgcc gttccctggc
     2281 cagtgcccgg tgaaatcagg caggaccagg atgagacggt gatcgagggt tccgccgaag
     2341 aggtcgatcc ctcggccgga gatctcgaag cgggcgagcc cgaagggtcc gatgaagggc
     2401 cgcagggcga gcttcgcaag cgcaagaaac ggaactgaag ccgcttcctt ctgagacgat
     2461 ggcggcatgg cctcgcagtc gctgaccgtc gccgaattca tcgagctccc gatcgtcaag
     2521 gccgggctac ccgaactcgt ggccgggagc gaaggtctgg acgcggaagt gcgatgggtc
     2581 catccgctcg atgtcccgga tgtgtccgat ctgcttcgcg gaggggagat gatcctcacc
     2641 accggagtct cgatcggaga ggacgcaacc gcgcagcggc gtttcgtccg cgacctcgaa
     2701 gccgaggggg cggtaggcat cgcggtcgaa ctcgggttcg cctggagccg ggagttgccc
     2761 aaggcgctgg ttgacgaggc cgaccggcgc cacatcccga tggtcgcctt tcgccgcggc
     2821 atccggttcg ttgaggtttc cgaagtcgtc aacggcagcc tgctcgactc cggccatgcc
     2881 ctggcccgtc gcggcgaaga actccaccgc agcctcgacc ggctggtgct ggaaggcgaa
     2941 ggggccgaat cggttctggc cgaggtgtcg aggaggatcg ggaacccggt cgtgctggag
     3001 gacgcccggg gcgagctggt cgcagtcggt tcggcaaccc gcccggagag tgaagtggtt
     3061 gacacctggt ccggactgaa atggtcggac cgcaatcacg ccgaggcgga gggagccctg
     3121 gccgcgcagg tcatggtccg cggccgcaac tggggccggg tgatcgcgct ccagaccgac
     3181 ggggaatttg accgcttcac gccgatcgcc ctcgaaagag cggcggtggc ggtcggtctg
     3241 ggcctgatga g
//
LOCUS       DAOQMF010000114         3240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16122_length_3240_cov_4.984929, whole genome shotgun sequence.
ACCESSION   DAOQMF010000114 DAOQMF010000000
VERSION     DAOQMF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16122_length_3240_cov_4.984929"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLV77_02800"
     CDS             complement(<1..706)
                     /locus_tag="PLV77_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14809.1"
                     /translation="MRSVRQAILLLFCLLAFAAAPAGGASANPAWKPHVNLARRWAGT
                     RSGEISFAVVTGGHRYMFHGLRMDPMASTVKVMLMTSYLRSRPVRRRNLHPGERRLIR
                     RMIRHSDDFAATEIRNRIGHSGLPHLARTVGIHAFIYSAIWGECRSNPRSQAGFMRRL
                     PDLLPRRHRRFALRQLNRITPSQRWGIGRVRPHGWRLYFKGGWGSGSGAVDHQIALLR
                     RGRHRIGLSIQTMNNPS"
     gene            complement(779..1033)
                     /gene="rpmA"
                     /locus_tag="PLV77_02805"
     CDS             complement(779..1033)
                     /gene="rpmA"
                     /locus_tag="PLV77_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNC14810.1"
                     /translation="MAHKKGLGSSRNGRDSNPKMLGVKVFAGETVSGGEIIVRQRGTK
                     FWPGEGTGLGRDHTIFATRPGTVEFKPGRRGRTISVAPAE"
     gene            complement(1023..1565)
                     /gene="rplU"
                     /locus_tag="PLV77_02810"
     CDS             complement(1023..1565)
                     /gene="rplU"
                     /locus_tag="PLV77_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNC14811.1"
                     /translation="MYAVVESGGKQYRVEKGTVLVVDRLKAEQGDKVALKPVMFKDKE
                     VIVDAKGLEKIKIEATVTEHLRGDKIKVFKYKPKKGYSRRAGHRAELTKLEVTSLGGG
                     AKKAAPKKAAADKAEEKPKAEAKAETAEKKAPAKKPAAKKPAAKAETKTETKAAAKKP
                     AAKKPAAKKPAAKKEDTDGA"
     gene            complement(1639..>3240)
                     /locus_tag="PLV77_02815"
     CDS             complement(1639..>3240)
                     /locus_tag="PLV77_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNC14812.1"
                     /translation="VGVSRRLADKERDRLRRMVSRTYDGPGGLIVRTAAHGAKKADFT
                     REIAYLHRLDAVLEKRREGAKAPSLVFQEQDLPVRVLRDVFLNEFDKAIIDDQKQFDR
                     VTSFFQRTAPELVEGVEHYKGKKPLFEKWEIERAIQSTLGRRIDLPSGGYLIIDYTEA
                     LTVIDVNSGSFTGRGKGGLEETITKVNTEAASEVVRQLRLRDIGGIIVIDFIDMAKGK
                     NRDKVLGTLREALDADKSKSYLVEISPLGLVEMTRQNITDGVREILTVQCPTCEGEGV
                     VLSAETVALEGLRKMKEIAADHAGDEAFLVRVNPKVAALLNDSDSGLLELEEETGKQF
                     HFEGGEALAIDNFELIESGEKSSIEERALPFRPGEEVFVKIEEPHMYNADDAIARIDS
                     YIISITGAANAVGERKMVRIQSVERASAVAELLPEEAQPKETKKAAPRKKNGSGQNAG
                     RSENGKASASGRGPKQETGNRGNDRQGRSGQGSKNGSDPKPKAESAVKKDAEGEKEDK
                     PVKSGPFGLRRGRRRRGKKKEDAGS"
BASE COUNT          458 a         1071 c         1033 g          678 t
ORIGIN      
        1 gggacgggtt gttcatggtc tggatcgaga gtccgatccg gtgcctgccg cggcggagca
       61 gggcaatctg atggtcgacc gcgccgctgc cgctgcccca gccgcccttg aagtagagcc
      121 gccatccgtg cggcctcacc cgaccgattc cccagcgctg ggacggagtg atccggttga
      181 gctgccgcag ggcaaaccgg cggtgacgcc gcggcaaaag gtccggaagg cgcctcatga
      241 aaccggcctg gctgcgcggg tttgaccggc actcgcccca tatcgccgag tagatgaaag
      301 cgtggatgcc gaccgtccgg gcgaggtgcg gcagtcccga gtgaccgatc cggttgcgga
      361 tctcggtcgc cgcgaagtca tcggagtgcc ggatcatccg cctgatcagc cgacgctcac
      421 cggggtgaag gttccggcgg cgaaccggcc ggcttctcag gtatgaggtc atcagcatca
      481 ccttgaccgt gctggccatc ggatccatgc gcagtccgtg gaacatgtag cggtggccgc
      541 cggtgaccac ggcgaaggag atctcgccgc tccgggtgcc ggcccatcgc cgggcgaggt
      601 tcacatgcgg cttccaggcc ggattagcac ttgcgccccc ggccggcgcg gcggcgaagg
      661 cgagcaggca aaagaggagc aggatcgcct ggcgaacgga acgcatccat tcacggtacc
      721 ccggaaaatg caaaagaaaa ggcccgcgga gcgggccttt ccgaatcctg tttcccggtt
      781 actcggccgg agcgaccgag atcgtcctgc cgcggcggcc gggcttgaac tcgaccgtgc
      841 cgggccgggt ggcgaaaatg gtgtggtcac ggccgaggcc ggtgccctcg ccgggccaga
      901 acttcgtgcc gcgctggcgg acgatgatct cgccgccgga aacggtttct ccggcgaaga
      961 ccttcacccc gagcatcttc gggttcgagt cacgaccgtt ccgtgaggat ccgagtcctt
     1021 tcttatgcgc catcggtgtc ctccttcttg gcggccggct tcttggccgc aggctttttg
     1081 gcggccggct tcttcgcggc agccttggtt tcagtcttgg tttcggcctt ggccgccggc
     1141 ttcttcgcgg ccggcttctt tgccggggcc ttcttctcgg cggtttccgc cttggcttcg
     1201 gccttcggct tctcctccgc cttgtcggca gcagccttct tcggggcggc cttcttcgcg
     1261 ccgccaccca gggaagtcac ctcgagcttc gtcaactcgg cccggtgacc ggcccggcgg
     1321 ctgtagccct tcttcggctt gtacttgaag accttgatct tgtcgccgcg cagatgctcg
     1381 gtgacggtcg cctcgatctt gatcttctcg aggcctttcg cgtcaacgat gacctccttg
     1441 tccttgaaca tgaccggctt cagggcgacc ttgtcaccct gctcggcctt caggcgatca
     1501 acgacgagca ccgtgccctt ttcgacccgg tactgcttgc caccactttc aactaccgcg
     1561 tacatgtctt gaactcaaat ccttgtctgg tcttcctgaa gctgagtctt ctcagcctga
     1621 aatcggcggg accgaggatc aactaccggc gtcttccttc ttcttgccac gccgacgtcg
     1681 tccgcgcctc aggccgaacg ggccggattt taccggtttg tcctcttttt caccttctgc
     1741 gtcctttttc accgctgatt cggctttggg tttcgggtcg gaaccgttct tcgagccctg
     1801 accggacctg ccctggcgat cgttaccgcg attcccggtc tcctgtttcg ggccgcgacc
     1861 cgacgcggaa gccttgccgt tttcgctcct gccggcattc tggcccgacc cgttcttctt
     1921 tcgaggggcg gccttcttcg tctccttggg ctgggcttcc tccggcagca gctcggccac
     1981 cgcggaggcc cgctcgaccg actggatgcg gaccatcttg cgctcaccga ccgcattcgc
     2041 ggcaccggtg atgctgatga tgtaggagtc gatccgggcg atcgcgtcat ccgcgttgta
     2101 catgtgcggc tcttcgatct tgacgaacac ttcctcgccc ggccggaagg gcagggccct
     2161 ttcctcgatc gaagacttct cgccggactc gatcagttcg aagttgtcga tcgccagggc
     2221 ctcgccgccc tcgaagtgga actgcttgcc ggtttcttcc tcgagctcga gcaggccgga
     2281 gtcggaatcg ttgagcagcg cggcgacctt cgggttgacc cggaccagga aggcctcgtc
     2341 accggcgtgg tcggccgcga tctccttcat cttgcggaga ccttccaggg cgaccgtctc
     2401 ggccgagagc accacgccct cgccctcgca ggtcgggcac tggacggtga ggatctcgcg
     2461 caccccgtcg gtgatgttct ggcgggtcat ctcgaccagg ccgagcggcg aaatctcgac
     2521 caggtagctc ttcgacttgt cggcgtcgag cgcttcccgc agggtgccga gcaccttgtc
     2581 gcggttcttg cccttcgcca tgtcgatgaa gtcgatgacg atgatgccgc cgatgtcgcg
     2641 cagcctgagc tgccggacga cttcggaggc cgcctcggtg ttgaccttgg tgatcgtctc
     2701 ctcgaggccg ccctttccgc ggccggtgaa ggaaccggag ttcacgtcga tcacggtcag
     2761 cgcctcggtg tagtcgatga tcaggtagcc gccggagggc aggtcgatac gacggcccag
     2821 ggtcgactgg atcgcccttt cgatctccca tttctcgaag agcggcttct tgcccttgta
     2881 atgctcgacc ccttcgacca gctccggcgc ggtgcgctgg aagaagctgg ttacccggtc
     2941 gaactgtttc tggtcatcga tgatcgcctt gtcgaactcg ttgaggaaga cgtcgcggag
     3001 cacgcggacc ggcaggtcct gctcctggaa gacgagggat ggcgccttgg cgccctcgcg
     3061 gcgcttctcg agcacggcgt cgaggcgatg caggtaggcg atctcgcggg tgaagtcggc
     3121 ctttttggcg ccgtgggccg cggtgcggac gatcaggccg cccgggccgt cataggttcg
     3181 gctgaccatg cggcgcaggc ggtcgcgttc cttgtcggcg agccggcggc tcacccccac
//
LOCUS       DAOQMF010000115         3240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16125_length_3240_cov_4.548509, whole genome shotgun sequence.
ACCESSION   DAOQMF010000115 DAOQMF010000000
VERSION     DAOQMF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16125_length_3240_cov_4.548509"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="map"
                     /locus_tag="PLV77_02820"
     CDS             complement(<1..747)
                     /gene="map"
                     /locus_tag="PLV77_02820"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNC14813.1"
                     /translation="MIIRKTDDQIEKMAAAGAVHARCMKMLTAKVRPGVTTAELDEVA
                     EKFIRSQGGVPTFKGFRGFPGSICASPNSMVVHGIPGPYKLQKGDILSIDIGVTLDGW
                     VADGASTIPVGPIDETTQKLLTVTKESLDLAAAQMVPGNRVGDIGHAVQSHVEANGFS
                     IIRSLVGHGVGQNMHEEPQIANYGTPGRGPEIEEGMVFAIEPMVNVGGPDVYMDDDGW
                     SVFSDDGSMAAHFEYTVAATKDGPRILTPWD"
     gene            complement(758..1432)
                     /locus_tag="PLV77_02825"
     CDS             complement(758..1432)
                     /locus_tag="PLV77_02825"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNC14814.1"
                     /translation="MAELNLILLGPPGSGKGTQGERLQEDLEYPYYATGDILRAAVKE
                     GTEVGNAAKEYMDRGDLVPDEVIIGIIAERLESGEADHGFILDGFPRTVVQAEALDAE
                     LHKLGRQLTAVILIDVEDDEIVNRLSGRRVCQEKGHVYHVDNSPPEQEGVCDVDGSPL
                     YIRDDDKPEVVRHRLEQYQEKTAPLVDYYKKQGLLNKVEGAEDPDRVSEKIRTLVSTL
                     SLEEGS"
     gene            complement(1443..2711)
                     /gene="secY"
                     /locus_tag="PLV77_02830"
     CDS             complement(1443..2711)
                     /gene="secY"
                     /locus_tag="PLV77_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNC14815.1"
                     /translation="MIATIAKAFSVPEIRRKLLFAAAMLALYRLGAYIPAPGIDVDAV
                     KELESQLTGSNILGFLNLFSGGSLSRLSLFALGIMPYITASIILQLLTVVVPSLERLQ
                     KEGEVGQQKITQYTRYLTVGLAAMQSFAYVFLFNSLSAGGNSVVSNMNLANVLLIVIS
                     LTAGTVLLMWIGELITQRGIGNGISLLIFASIVSGVLPGIQTWWNNPDQVFVVLLPFI
                     CLAVIVAIVFVQEGQRRIPVQYAKRVVGRKMTSGGSTYLPLRVNMAGVIPVIFAASIM
                     ALPPTIGQLINTPAALDFAAFLSPSSWAYVVGEVFFIIIFTYFYTAVTFNPVDQADNL
                     KKYGGFIPGVRPGRPTAEYLDRVLSRLTFPGALYLGAVAAFPTILFNNVGGGFFFGGT
                     SILIVVGVALDTVKQLEAQLMMRNYEGFLR"
     gene            complement(2711..3178)
                     /gene="rplO"
                     /locus_tag="PLV77_02835"
     CDS             complement(2711..3178)
                     /gene="rplO"
                     /locus_tag="PLV77_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNC14816.1"
                     /translation="MGLHNLSPKPGSRRPKKRIGRGPGSGTGKTSGRGHGGQGQRSGN
                     KRKVNLEGGQTPIHMRMRKLRGPHMKKSMPFENFRTATQPVNLGDLEDRFDAGAEVTP
                     ETLKAAGLATRKDPVKILARGEISKSLTVKAHGFSAAAKEKIEAAGGSCELID"
BASE COUNT          559 a          990 c         1101 g          590 t
ORIGIN      
        1 gtcccagggg gtgaggattc gggggccgtc cttggtggcg gcgaccgtgt attcgaaatg
       61 ggcggccatc gagccgtcgt cggagaagac cgaccagccg tcatcgtcca tgtagacgtc
      121 ggggccaccg acgttgacca tcggttcgat cgcgaagacc attccctcct cgatctcggg
      181 gccgcggccg ggagtgccgt aattggcgat ctgcggctct tcgtgcatgt tctgccccac
      241 gccgtgaccg accagggaac ggatgatcga gaagccgttc gcctcgacgt ggctctggac
      301 cgcgtggccg atgtccccca cccggttgcc gggcaccatc tgcgcggcgg cgaggtcgag
      361 cgactccttg gtcacggtga gcagcttctg cgtggtctcg tcgatcgggc cgaccgggat
      421 ggtcgaggcg ccgtcggcga cccagccgtc gagggtaacc ccgatgtcga tcgagaggat
      481 gtcgcccttc tgaagcttgt agggcccggg gatgccgtga acgaccatcg agttggggga
      541 ggcgcagatc gaaccgggga aaccgcggaa acccttgaag gtgggcaccc cgccctggga
      601 gcgaatgaac ttctcggcca cctcgtcgag ttcggccgtg gtcacgccgg gccgcacctt
      661 cgcggtcagc atcttcatgc accgcgcgtg gacggcaccc gcggccgcca tcttctcgat
      721 ctgatcgtcc gtcttgcgga tgatcatggg ggtgagatca gctgccttct tcgaggctga
      781 gcgttgaaac cagggtgcgg atcttctcgc tgacccggtc gggatcttcg gcgccctcga
      841 ccttgttcag cagaccctgc ttcttgtagt aatcgaccag cggcgcggtc ttttcctggt
      901 actgctcaag ccggtgccgg accacttccg gcttgtcgtc atcacggatg tagagcgggg
      961 agccgtcgac gtcacagacg ccttcctgct cgggcgggct gttgtccacg tggtagacat
     1021 ggcccttctc ctggcagacg cggcggccgg aaaggcggtt gacgatctca tcgtcctcga
     1081 cgtcgatcag gatcaccgcc gtgagctggc ggcccagctt gtgaagttcg gcgtcaagcg
     1141 cctcggcctg cacgaccgtg cgggggaagc cgtcgaggat gaaaccgtga tcggcctcgc
     1201 cggactcgag ccgctcggcg atgatgccga tgatcacctc gtccggaacc aggtcacccc
     1261 ggtccatgta ctccttcgcg gcgttgccga cctcggtgcc ctccttgacc gcggcccgga
     1321 ggatgtcccc ggtcgcgtag tacgggtact cgagatcttc ctgcagacgc tcgccctggg
     1381 tgcctttacc gctcccgggc gggccgagca ggatcaggtt gagttcggcc acgggtaacc
     1441 cgctacctga ggaagccctc gtagttgcgc atcatcagct gcgcctcgag ctgcttgacc
     1501 gtgtcgaggg ccacgcccac gacgatcagg atcgaggtgc caccgaagaa gaacccgccg
     1561 cccacgttgt tgaagaggat ggtcgggaaa gccgcaacgg cgccgaggta gagggcgcca
     1621 gggaaggtca ggcgggagag cacgcggtcc aggtattcgg cggtcggccg gcccgggcgc
     1681 acgccgggaa tgaacccgcc gtacttcttg aggttgtcgg cctggtccac cggattgaag
     1741 gtgacggccg tgtagaagta ggtgaagatg atgatgaaga acacttcacc gaccacgtag
     1801 gcccagctcg acggactgag gaaggcggcg aagtcaagcg cggcgggagt gttgatcagc
     1861 tgaccgatgg tcggcggcag ggccatgatc gaggcggcga agatgaccgg gatgacgccg
     1921 gccatgttga cccggagcgg caggtaggtc gaaccgcccg aggtcatctt gcgaccgacc
     1981 acgcgcttgg cgtactgcac cgggatccgg cgctgacctt cctggacgaa gacgatcgcg
     2041 acgatcacgg ccaggcagat gaacggcagc agcacgacga agacctgatc ggggttgttc
     2101 caccaggtct ggatgccggg caggacgccg gagacgatcg aggcgaagat caggagtgaa
     2161 atgccgttgc cgatcccgcg ctgggtgatc agctcgccga tccacatcag caggacggtt
     2221 cccgcggtca gcgagatcac gatcagcaga acgttcgcga ggttcatgtt cgaaacgacg
     2281 ctgttgccgc cggccgagag cgagttgaag aggaagacgt aggcgaagga ctgcatcgca
     2341 gccaggccga cggtcaggta acgcgtgtac tgcgtgatct tctgctggcc gacttcgcct
     2401 tccttctgga gacgctccag cgaaggcacg accacggtca ggagctgcag gatgatcgag
     2461 gccgtgatgt agggcatgat gcccagcgcg aaaagcgaaa gcctcgagag cgagccgccc
     2521 gaaaacaggt tcaggaagcc gaggatgttc gatccggtca gctggctttc gagttccttg
     2581 acggcatcga cgtcgatgcc gggggccgga atgtaggcac cgagccggta cagggccagc
     2641 atggccgctg cgaagagaag cttccggcga atctccggga cggagaatgc tttcgcaatc
     2701 gttgcgatca tcagtcgatc agctcgcagc ttccgccggc ggcttcaatc ttctccttgg
     2761 ccgcggccga aaagccgtgg gccttgacgg tcaggctctt tgaaatctcg ccgcgggcga
     2821 ggatcttgac cggatccttg cgggtcgcca gaccggcagc cttgagcgtt tccggggtga
     2881 cttccgcacc ggcgtcgaag cgatcttcga gatcgccaag gttgaccggc tgggtcgcgg
     2941 tccggaaatt ttcgaacggc atcgacttct tcatgtgcgg gccgcgcagc ttgcgcatcc
     3001 gcatgtgaat cggggtctgg ccaccttcga ggttgacctt ccgcttgttg cccgagcgct
     3061 ggccctggcc gccgtgtccg cggccggagg tcttgccggt gcccgaaccg ggtccgcggc
     3121 cgatccgctt cttcggacgg cgcgagccgg gcttggggct cagattgtga aggccgattt
     3181 cttcttcgcg gctttcctgc ttgtcagcca tcaggcctcc tccacctcga ccaggtggga
//
LOCUS       DAOQMF010000116         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16290_length_3222_cov_3.115567, whole genome shotgun sequence.
ACCESSION   DAOQMF010000116 DAOQMF010000000
VERSION     DAOQMF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16290_length_3222_cov_3.115567"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..927
                     /locus_tag="PLV77_02840"
     CDS             166..927
                     /locus_tag="PLV77_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC14817.1"
                     /translation="MSPVEIAILLVWSFTVSFAGGMVGLVLGNLRLPVVVWLGTSTAA
                     AAGANVAISGLAALTATLVHIRNGRLDPRLFLWMAPSSFLGAVAGGVLAGVLPERILL
                     GAISLVVLYGAVEVWRYRRPKGDGEGNRPDRRRLLTEAVLIGFGVGLLGGFVGLILGS
                     LRLPAMLKYMGMSPQKAVGTNSAVGVIVGIGGLLGHLPSGVDWNILLIGAAAAVPGAA
                     FGSKLTGKLPEATLVRAMAFVLLVTGLAMAGQAII"
     gene            934..2595
                     /locus_tag="PLV77_02845"
     CDS             934..2595
                     /locus_tag="PLV77_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14818.1"
                     /translation="MDSTGREQLKRHLPEIILGLLMLAQASFLLVLTAPIAFVSDEWQ
                     FLFFRQGFSPGTTLDPYYEHLVAVPNYLYSTAVGIFGLGTNRPMQIAALLTFLGLNAL
                     LFVYLRRRAGGWAALIGTALILFLGAAFEDLLFAFQINYFGAIACGLGALLALDRDDR
                     RGDLIASLLLVAGVLTSSMIFPFIAAAALEWALNPRDRRRRLFVPGAAIGIWILWWIG
                     WGHQADSQAGLSGLSGLPGYLWKAVPAGFTSLAGLATDDGTMPDQPHLIWGRLLCLAA
                     LAAAGWRIYRLGRVPRELWVAIAAAATFFVLSGLNASDFRLPTSSRYQLPSALFIVLI
                     AANLLRGVKIPGRALVASAILAALAVFGGARLMEEQATERWQPTSRSARAALGAIDLA
                     GPAVIPGYQIDLRTNPEQDRLIPIDLYRETTASHGSPGMDASEIEGSAPQYRLQADLA
                     LIGATGIHLSGLPVRPSGPCQRVRGSNNLFGPVPLQSGGYLIMNRGKDQLAVALSRFS
                     DLPGVSAGAILPETMAGLDLPQDNSTRPWQAVFFGEGPVRICPTG"
     gene            2621..>3222
                     /locus_tag="PLV77_02850"
     CDS             2621..>3222
                     /locus_tag="PLV77_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14819.1"
                     /translation="MRNPGQSRFLPWVLLGLLMLGSGAILLSVTSGINFVTDEWNLLF
                     LRSGWTPDSFLEPFHEHVIIAPTLIYKVLQGTLGMDSNRPFQLAALIMFLAIGPLLFI
                     WMRRRIGDWAALIGVALILFLGAAFEDLLWAFQIGYFGSLAFGLAALIALDRDDRRGD
                     VAAAILLVLSLSFSSLGLPFVAGAAAEWLTNPRERGKRWLV"
BASE COUNT          423 a         1102 c         1088 g          609 t
ORIGIN      
        1 ggagcgagga tgtgatcctg cccgaccacc tcgtccagcg ataccggtcg catccgggcc
       61 gccaggggcg cgtccggccc gacctgcgga atgccggaac cgccgggatc cacttcacct
      121 gaatcaaaca attggtcggc caccgcatga gattattccc ggttggtgag tccggttgaa
      181 atcgccatcc tgctggtctg gtcattcacc gtctccttcg ccggtggaat ggtcgggctg
      241 gtgctgggca acctgaggct gccggtcgtg gtctggctcg gcacttcgac cgcggccgcc
      301 gccggagcga acgtggcgat ctccgggctg gccgcgctga ccgcaaccct ggtccacatc
      361 agaaacgggc ggctggaccc ccgcctcttc ctctggatgg caccgagctc gttcctcggc
      421 gcggtggcgg gcggtgtcct cgccggggtg ctgccggaaa ggatcctgct cggggcgatc
      481 agcctggtcg tgctttacgg cgcggtcgag gtctggcgat accgccggcc gaaaggcgat
      541 ggggagggga accgccccga ccggcgccgg ctcctgaccg aagcggtgct gatcggtttc
      601 ggggtcgggc tgctgggcgg attcgtcggt ctgatcctag ggtcgctgcg gctgccggcg
      661 atgctcaagt acatgggcat gagcccgcag aaggcggtcg gcaccaactc cgcggtcgga
      721 gtgatcgtcg ggatcggcgg cctgctcggg caccttcctt ccggggttga ctggaacatc
      781 ctgctgatcg gcgcggccgc ggcggtcccg ggtgccgcgt tcggttcgaa gctgaccgga
      841 aagctgcccg aggcgaccct ggtccgggcg atggcattcg tcctgctggt aacgggactc
      901 gcgatggccg ggcaggcgat catctgaccg tcgatggatt cgacggggag agagcaactg
      961 aaaagacacc tgcccgagat cattctcggt ctgctgatgc tggcccaggc gagctttctc
     1021 ctggtgctga ccgcaccgat cgccttcgtc agtgacgagt ggcagttcct cttcttccgc
     1081 caggggttca gccccggcac cacgctcgat ccctactacg aacacctggt cgcggttccg
     1141 aactacctct actcgaccgc ggtcgggatc ttcgggctgg gcaccaaccg gccgatgcag
     1201 atcgcggccc tgcttacctt cctcggcctg aacgcactgc tcttcgtcta cctgcgccgc
     1261 cgggcaggcg ggtgggcggc cctgatcggc actgccctga tcctcttcct cggggctgcc
     1321 ttcgaggatc tgctcttcgc cttccagatc aactacttcg gagcgatcgc ctgcgggctc
     1381 ggcgccctcc tggcgctgga ccgggatgac cggcggggcg atctgatcgc ctcgctgctg
     1441 ctggtcgccg gcgttctgac ctccagcatg atctttccgt tcatcgccgc cgccgcgctc
     1501 gaatgggccc tcaacccgag ggaccggcgc cggcggctct tcgttccggg cgcggcgatc
     1561 ggcatctgga tcctgtggtg gatcggatgg ggccatcagg ccgactcgca ggccgggctc
     1621 tccggactga gtggtctgcc cggctacctc tggaaggcgg tcccggccgg tttcacctca
     1681 ctggccgggc tggcgaccga tgacggcacc atgcccgacc agcctcacct gatctggggg
     1741 cgcctgcttt gcctggccgc cctggccgcg gcgggctggc ggatctaccg gctggggcgg
     1801 gtgccacggg agctctgggt ggcgatcgcg gccgccgcta ccttcttcgt gctctccggc
     1861 ctcaacgcca gtgatttccg cttgccgacc tccagccggt accagcttcc ctcggcgctt
     1921 ttcatcgtcc tgatcgcggc caacctgctg cgcggggtga agatccccgg acgcgccctt
     1981 gtcgcctcgg cgatcctggc tgcgctggcg gtcttcgggg gagccaggtt gatggaggaa
     2041 caggcgacgg aacgctggca accgacctcg cgctcggccc gggccgcgct gggtgcgatc
     2101 gacctggccg gtccggccgt gatcccggga taccagatcg acctgcggac gaatcccgaa
     2161 caggaccgcc tgatcccgat cgatctctac cgggagacga ccgccagcca cggatcaccg
     2221 gggatggatg ccagcgagat cgaaggctcc gccccgcagt accggcttca ggccgacctc
     2281 gccctgatcg gggcgaccgg aatccatctc agcggcctgc cggtccgtcc ctccggcccc
     2341 tgtcagaggg tgcgcgggtc gaacaacctc ttcgggccgg tccccctcca gtcgggcggc
     2401 tacctgatca tgaaccgggg caaggaccag ctggcggttg ccttgagccg gttttccgac
     2461 ctgcccgggg tgagtgccgg cgcgatcctc ccggagacga tggccgggct ggacctgccg
     2521 caggacaatt cgacgaggcc ctggcaggcg gtcttcttcg gcgagggccc ggtccggatc
     2581 tgcccgaccg ggtgagcctt ccgggagtag gttgaccggc atgcggaacc caggccagtc
     2641 ccgttttctg ccgtgggttc tgctcggcct gctgatgctc ggttcgggag cgatcctgct
     2701 ctcggtcacc tcggggatca atttcgtcac cgacgaatgg aacctgctct tcctccggtc
     2761 cggctggacc ccggacagct tcctggagcc cttccacgag cacgtgatca tcgcccccac
     2821 cctgatctac aaggttctcc aagggaccct ggggatggat tcgaaccggc ccttccagct
     2881 tgctgcgctg atcatgttcc tggcgatcgg cccgttgctc ttcatctgga tgcggagacg
     2941 gatcggggac tgggcggcgc tgatcggggt cgccctgatc ctctttctgg gggcggcctt
     3001 cgaggacctg ctctgggcct tccagatcgg ctacttcggg tcgctggcct tcggcctggc
     3061 cgcgctgatc gcactcgatc gcgacgaccg ccggggggat gtcgcggcgg cgatcctgct
     3121 ggtcctttcc ctcagcttct cctcgctcgg cctgcccttc gtggccggcg cggccgccga
     3181 gtggctgacc aatccccgtg agcgggggaa gcgctggctg gt
//
LOCUS       DAOQMF010000117         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16342_length_3216_cov_4.186650, whole genome shotgun sequence.
ACCESSION   DAOQMF010000117 DAOQMF010000000
VERSION     DAOQMF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16342_length_3216_cov_4.186650"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLV77_02855"
     CDS             complement(<1..741)
                     /locus_tag="PLV77_02855"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNC14820.1"
                     /translation="MSVKVAINGFGRIGRNVFRAAHDSGADLDIVAINDLVDTETLAH
                     LLKYDSILGRLDAEVSAGEGSITVDGKEIKVLAERDPADLPWGDLGIDVVLESTGFFT
                     KRDDAAKHLSAGAKKVIISAPATDPDVTVALGVNFDDYDPENHNIISNASCTTNCLAP
                     FAKAINDAVGIEQGLMTTIHAYTSDQRLQDMPHKDLRRARAAAQNLIPTTTGAAKAVG
                     LVLPDLAGKLNGFAVRAPVITGSVVDLTF"
     gene            complement(1048..1410)
                     /locus_tag="PLV77_02860"
     CDS             complement(1048..1410)
                     /locus_tag="PLV77_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14821.1"
                     /translation="MIHHIVLEVSDLPRASSFYDALLSPLGWRRHVNETNAVGYGISR
                     PILFITERTGVRAEGTMVSLGAPGIAAVKAAWEGGTNSGGSNVAQPGETSAHGSGSYS
                     AFLRDPDGHDIEITVSQD"
     gene            complement(1524..2294)
                     /locus_tag="PLV77_02865"
     CDS             complement(1524..2294)
                     /locus_tag="PLV77_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14822.1"
                     /translation="MKPISLALHEFRFAQKVFWRNPASVFFTALLPVLFLLIFATIFG
                     GDQIEELGGVKVTTYYIPAIVTLAVISATMVNLSMNVTIAREQGLLKRGRGTPLPPWV
                     FIAGRVGNAIVISAVMLVVVTLIGKLLYDAPIPWERIPAVLVTLVVGATSFCCLGLAM
                     TVIIPSREAASAISNAITLPLYFLSGIFIPENEIPEGVLSFANHFPVRDFFEAFFSAY
                     SPKTVGAGFEWGELAVVAIWGLAGFLIALRYFRWTPRQ"
     gene            complement(2291..>3216)
                     /locus_tag="PLV77_02870"
     CDS             complement(2291..>3216)
                     /locus_tag="PLV77_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC14823.1"
                     /translation="LAVSSAAISIKGLRKTYDDLEAVRGVDLEVFPEEIVAVLGPNGA
                     GKTTTVEILEGYRDRDGGEVSVLGVDPAKPTPAWRDRIGIVLQSTTLEPALTVRESLQ
                     QYAGYYRNPREVDEVIGLVGLEEQAGTRARKLSGGQQRRLDVGIALVGDPDLLFLDEP
                     TTGFDPSARRQAWEMVAGLRGLGKTVLLTTHYMEEAQFLADRVAIMSEGRIVALDTPE
                     NLGDRRRMPTTISFSVPAGTDLAGIAGVGQTQSSSEDDTGLRTVSIGTHEPVADLNRI
                     TGWAIESDLEIQHLEVRRPSLEDVYLELTDA"
BASE COUNT          563 a          976 c         1120 g          557 t
ORIGIN      
        1 gaaggtcagg tcgacgaccg aaccggtgat caccggagca cgaaccgcga agccgttcag
       61 cttgccggcc aggtcgggca ggaccagtcc gacggccttg gccgcaccgg tggtggtcgg
      121 gatcaggttc tgcgcggcag cacgggcccg gcgcaggtcc ttgtggggca tgtcctgaag
      181 ccgctggtcc gaggtgtagg cgtgaatcgt ggtcatcagg ccctgctcga tgccgaccgc
      241 gtcattgatc gccttggcga agggggccag gcagttggtg gtgcaggatg cgttcgagat
      301 gatgttgtgg ttctcggggt cgtaatcgtc gaagttgact ccgagcgcca cggtcacgtc
      361 gggatcggtc gccggcgccg agatgatgac cttcttcgcg cctgccgaca gatgcttggc
      421 ggcgtcgtcg cgcttggtga agaaaccggt cgactcgaga accacgtcga ttccaaggtc
      481 gccccagggc aggtcggccg ggtcacgctc ggccaggacc ttgatctcct tgccgtcgac
      541 ggtgatcgag ccctcgccgg cgctcacctc ggcgtcgagg cgcccgagga tcgagtcgta
      601 cttgagcagg tgcgcgaggg tctcggtgtc gaccaggtcg ttgatcgcga cgatgtccag
      661 atcagcaccg gaatcgtgcg ctgcccggaa aacgttccgg ccgatccggc caaaaccgtt
      721 gattgccacc ttgacgctca ttcgctaccg cctcctggga aaactgggtt ggtgaaactc
      781 cgattacacc ttggattcta cggccccgcc acccccggtg ccctgcggtc aaacaccgaa
      841 atcaggggtg ggtcggattt ccgtccctgc tgccggttat ccgacccact gccggtgaac
      901 agcggcattg acgggggaat gtggggtcac ccgtagacct gtggcggctc cgagtccggt
      961 tcaggcccga gtttcgggag gtgcctatgg atttcgtcta tatgaacgaa atccataggc
     1021 acctgccccg gatcgaggcc gggacctcta gtcctgggag acggtgatct ctatgtcgtg
     1081 accgtcaggg tcgcggagga aggcggagta ggagcccgat ccgtgggcgc tggtttcgcc
     1141 gggctgggcg acgttcgagc cgccggagtt ggttccgcct tcccaggcgg ccttgacggc
     1201 cgcgatgccg ggggcgccga ggctgaccat cgtgccttcg gcccggactc cggttcgttc
     1261 ggtgatgaag aggatcggac gggaaatgcc gtatccgacc gcgttcgttt cgttcacgtg
     1321 gcgtcgccac ccgagtggcg agagcagcgc gtcatagaac gaggaggcgc gagggagatc
     1381 ggaaacttcg aggacgatgt ggtgaatcac tcacggcagg ctaatggaga tgcaacaaaa
     1441 cgggaacctg accgttggcg agtggtcgag ggcggccggg cggaacggtg aaatccggtc
     1501 cctggatttg cggcgccggg cgactactgc cggggggtcc agcggaagta gcgcaaggcg
     1561 atgaggaatc cggccaggcc ccagatggcg accaccgcga gttcgcccca ctcgaaaccc
     1621 gccccgacgg tcttcgggct gtaggcggag aagaaggcct cgaagaaatc ccgcaccggg
     1681 aagtggttgg cgaatgagag gacaccttcg gggatctcgt tttcggggat gaagattccg
     1741 gaaaggaaat agagcggaag ggtgatcgca ttggagatcg ccgaagcggc ttcgcgcgag
     1801 ggaatgatca cggtcatcgc caggccgagg cagcagaacg aagtcgcgcc gacgaccagg
     1861 gtcacgagca cggccggaat ccgttcccag gggatcggcg cgtcatagag cagcttcccg
     1921 atcagcgtga ccacgaccag catcacggcc gagatcacga tcgcgttgcc gacccggccg
     1981 gcgatgaaga cccagggcgg cagcggggtt ccgcggccac gcttgagcag accctgctcc
     2041 cgggcgatcg tgacgttcat cgacaggttg accatggtcg ccgagatcac ggcgagggtg
     2101 acgatcgccg ggatgtaata ggtggtcacc ttgactcccc ccagttcctc gatctggtcg
     2161 ccgccaaaga tcgtggcgaa gatcagcaga aagagcaccg gcaggagcgc cgtgaagaag
     2221 accgaagcgg ggtttcgcca gaagaccttc tgggcaaagc ggaactcgtg cagtgccagg
     2281 gagatcggct tcatgcgtcg gtcagctcca ggtagacatc ctcgaggctg ggccggcgga
     2341 cttcgagatg ctggatctcg aggtcgcttt caattgccca gccggtaatc cggttgaggt
     2401 cggcgaccgg ctcgtgagtg ccgatactga ccgtgcggag gccggtgtcg tcttcgctgc
     2461 tcgactgggt ctggccgact ccggcgatgc ccgccaggtc cgttccggcc ggcaccgaga
     2521 aggagatcgt ggtcggcatg cgacggcggt caccgaggtt ctccggggtg tcgagggcga
     2581 cgatccgccc ttcgctcatg atcgccaccc ggtcggcgag gaactgggcc tcctccatgt
     2641 aatgggtggt cagcagcacg gtcttgccga ggccgcggag cccggccacc atctcccagg
     2701 cctgccggcg ggccgacggg tcgaagccgg tggtcggctc gtccagaaag agcaggtcgg
     2761 ggtcgccgac cagggcgatg ccgacgtcga gccggcgctg ctggccgccg gacagtttgc
     2821 gggcacgcgt cccggcctgt tcctcgagac cgaccagtcc gatcacctcg tcgacctcgc
     2881 ggggattgcg gtagtagccg gcatattgct gaagcgattc cctgaccgtc agggccggct
     2941 ccagcgtggt gctctgcagc acgatcccga tccggtcccg ccaggcgggg gttggtttgg
     3001 cgggatccac gccgaggaca ctgacttcgc ccccgtcgcg gtcacggtag ccctcgagga
     3061 tctcgaccgt ggtcgtcttc ccggccccgt tcgggccaag cacggccacg atctcctcgg
     3121 ggaagacctc gagatcgact ccccggaccg cctcaagatc gtcgtaggtc ttgcgcaggc
     3181 ccttgatgct tattgccgca ctggacacgg ccaaca
//
LOCUS       DAOQMF010000118         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16476_length_3201_cov_3.171647, whole genome shotgun sequence.
ACCESSION   DAOQMF010000118 DAOQMF010000000
VERSION     DAOQMF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16476_length_3201_cov_3.171647"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLV77_02875"
     CDS             <1..1049
                     /locus_tag="PLV77_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNC14824.1"
                     /translation="DTAGTEGPGTFDQAISRKLVATGKDGKPFLLPSPFPGGQAAVID
                     LTKPAAVRWWRNRIWNLLDLGADGFMNDFGEQVEAGMRFHDGTSAALMHNRYPALQAK
                     VTRAAVDAWERRHPKRHIFFFQRAGFTGSLSSVKWESAQFPGDETVDWGSDTGLPSIV
                     PDMLNRALTGAVGFTTDIGGYSQFRTEQPFIPPTGEELFTRWAQAAVFTPFFRVHNSG
                     LSGARMPWDFSTDAQSTWKKMVVLHNRSRPLIRRLWKRFLKTGMPMIRPLYLAGGIGP
                     KNPHNDDEWMVGHDLLVAPVLEEGAVTREVLLPPGCWRRSGTGPPVEGARTVEVGAAL
                     DELPWFTRCGTNPL"
     gene            complement(1087..1170)
                     /locus_tag="PLV77_02880"
     tRNA            complement(1087..1170)
                     /locus_tag="PLV77_02880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1134..1136),aa:Leu,seq:cag)
     gene            1309..1381
                     /locus_tag="PLV77_02885"
     tRNA            1309..1381
                     /locus_tag="PLV77_02885"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1343..1345,aa:Glu,seq:ctc)
     gene            1469..2860
                     /locus_tag="PLV77_02890"
     CDS             1469..2860
                     /locus_tag="PLV77_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC14825.1"
                     /translation="MTRRDLGKAALAGAAVAGLGGAAGLAGCAREGGGNGSGASKQEV
                     IDGLPETGIPRPVDYIRTDWSHDPLAFCSYSCLAPTPYGSEIRRMLAEPVGRIAFAGE
                     ATARIPATVHGAIQAGRDAGKYAAGQLRAGARVAVVGAGAAGLSCAGELERLGLKPVV
                     LEASDRVGGRVREADLDGSPVELGASWIHGVDGNPLTELAGEAGVGLHPFRYQLDFPV
                     PGQQALGAGGEHRYWQAVNSFGPRSVRHPDVPLEAILPRRRGPGLEWAIQDEVAQEYG
                     ADPDQLAALATWEGNWLPGGDALLDGPYSQVIGSLSGDAGIRFGFEVRKISQRPGEVR
                     LTGAEDNDEVAADAAVVTVPIGVLKAERIEFDPPLPARTRTAIDGLGAGLLDKVWLAF
                     EEPFWDENAEGFSWIDPGRPGRWGAWVNAVPVTGEPFLLTLSGGRDAHRLSRQSDLEI
                     ISGTTAALNSMFG"
     gene            complement(2857..>3201)
                     /locus_tag="PLV77_02895"
     CDS             complement(2857..>3201)
                     /locus_tag="PLV77_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14826.1"
                     /translation="PSNRIFKVRKVTSRGDGSALIKVAVPGAGVVAADDAGKSDLVQR
                     AWQKSSKARTVSLVVKAGSKLRKALVASGKPRQVRVRISFTAKGSPKATPTTSTVRSI
                     SFLISPPQTLAN"
BASE COUNT          531 a         1020 c         1118 g          532 t
ORIGIN      
        1 atgacaccgc gggcacggaa ggccccggca ccttcgacca ggcgatttcc cgcaagctgg
       61 ttgcgaccgg caaggacggg aagccgttcc ttttgccctc gcccttcccg ggcggtcagg
      121 cggcggtgat tgacctgacc aaaccggccg cggtgaggtg gtggcggaac cggatctgga
      181 atctgttgga cctcggcgcc gacggtttca tgaatgactt cggcgaacag gtcgaagccg
      241 gcatgagatt tcacgacggg accagtgccg cgctgatgca caaccgctac ccggccctgc
      301 aggcaaaagt cacacgagcg gccgttgacg cctgggagcg ccggcatccg aaacggcaca
      361 tcttcttctt ccagcgggct ggattcaccg gctcgctcag ttcggtgaag tgggaaagcg
      421 cccagttccc gggtgacgag acggttgact ggggcagcga cacggggctg ccttcgatag
      481 ttccggacat gctgaaccgg gcgctaaccg gggcggtcgg tttcacgacc gacatcggcg
      541 gctactccca gttccggacc gagcaaccct tcatcccgcc aaccggagaa gaactgttca
      601 cgaggtgggc ccaggccgct gtcttcaccc cgttcttccg ggttcacaat tccggtctga
      661 gcggggcgcg gatgccgtgg gacttctcga ccgacgctca gtcaacctgg aagaagatgg
      721 tggtcctgca taaccggagc cggccgttga tcagaaggct ctggaagcgg ttcctgaaaa
      781 ccggcatgcc gatgatcagg cccctctatc tggccggcgg gatcgggccg aagaatccgc
      841 acaacgatga cgaatggatg gtcggtcacg accttctcgt cgcgcccgtc ctcgaagaag
      901 gcgcggtgac ccgggaagtg ctgctgccac ccggttgctg gcgccggtca ggtaccggcc
      961 cgccggtcga gggcgccagg acggttgagg tgggcgccgc ccttgacgag cttccctggt
     1021 tcacccgttg tggcaccaat ccgctctagg ccggaagccg gcccggcaag cgggccttcc
     1081 ggggaatgcg gctgagagga gtcgaacctc cacgttcttg cgaacacagg cacctgaagc
     1141 ctgcgcgtct accagttccg ccacagccgc gcggaggcgg aactctaccg gcttgacccg
     1201 ctgcggcgca ctccggctgt ggccgggcgg gcttgatctc cgcaaggccc cggcgtgacc
     1261 ccgccgaccg tccgttgagc atccgaattg ttagaatgct cgctgcgcgc cgctatcgtc
     1321 caggggtcta ggacgccgga ttctcagtcc ggaaaccggg gttcgaatcc ccgtagcggt
     1381 acctccgaaa gcccgcccgc cggcgggttt tcccgttttc agaccggatc ggcatgacat
     1441 cggcgacaat cggagcgtgg gattcgagtt gaccagacgg gacctgggca aggcggccct
     1501 cgcaggcgcc gctgtggccg ggctcggagg agcggccgga ctggccggtt gtgcccggga
     1561 aggcggtggc aacggatccg gagcctcgaa gcaggaggtg attgacggtc ttccggaaac
     1621 cgggatcccc aggccggtcg actacatccg gaccgactgg tcgcatgacc cgctggcctt
     1681 ctgctcctac tcctgcctcg ccccgactcc ctacggttcg gagatcagaa ggatgctggc
     1741 cgagccggtc ggcaggatcg cctttgccgg ggaagccacg gcgaggattc cggccacggt
     1801 tcacggggca atccaggcgg gaagggacgc aggaaagtac gcggccgggc agctcagggc
     1861 cggggcgcgg gtggcggtgg tcggggccgg ggcggccggc ctctcctgcg ccggtgaact
     1921 cgaacgcctc ggcctgaagc cggtggtgct tgaagcttcg gaccgggtcg gaggaagggt
     1981 cagggaggcc gacctcgacg gttcgccggt tgaacttggt gcctcgtgga tccacggcgt
     2041 ggacggaaac ccgctgaccg aactcgccgg ggaggccggt gtcgggcttc acccgttcag
     2101 gtaccagctc gatttcccgg tgccggggca gcaggcgctc ggagcggggg gcgagcatcg
     2161 ctactggcag gcagtcaatt ccttcggtcc ccgctcggtc cgccatccgg acgttccgct
     2221 cgaggcaatc cttccgcggc ggcgcggacc cgggctcgag tgggcgatcc aggacgaggt
     2281 cgcccaggaa tacggggccg acccggatca gctggcggcg ctcgcgacct gggaaggcaa
     2341 ctggctgccc ggaggtgacg ccttgctgga cgggccctac tcgcaggtga tcggaagcct
     2401 gtccggtgac gccgggatcc gattcgggtt cgaggtgcgg aagatcagcc agcggccggg
     2461 ggaggtccgg ctgaccgggg cagaggacaa cgatgaagtt gcggccgacg cggccgtggt
     2521 cacggttccg atcggggtac tgaaggcgga aaggatcgag ttcgacccgc cactgccggc
     2581 ccggacccgt accgcgatcg acggactcgg ggcgggtttg ctcgacaagg tctggctggc
     2641 cttcgaggaa ccgttctggg atgaaaacgc cgaaggtttc agctggatcg acccggggag
     2701 accgggacgc tggggtgcct gggtgaacgc ggtgccagtc accggagagc cgttcctgct
     2761 caccctctcc ggcggcaggg acgcccaccg gctttcccgt cagagcgatc tcgaaatcat
     2821 cagcggcacg acggccgcgc tcaactcgat gttcggctaa ttcgcgagcg tctgcggggg
     2881 cgagatcagg aacgagatcg aacgcacggt cgaagtcgtc ggggtggcct tcgggctgcc
     2941 cttcgccgtg aagctgattc tcacccggac ctgcctcggc ttgccgctcg cgaccagagc
     3001 cttgcggagc ttgcttccgg ctttcacgac cagcgaaaca gtgcgggctt tggagctctt
     3061 ttgccatgcc cgttgaacca ggtcggactt tccggcgtca tcggcagcca ccactcctgc
     3121 tccggggacg gcgaccttga tcagcgccga accgtcgccg cgactggtca ccttgcgaac
     3181 cttgaagatc ctgttcgacg g
//
LOCUS       DAOQMF010000119         3169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16749_length_3169_cov_3.336545, whole genome shotgun sequence.
ACCESSION   DAOQMF010000119 DAOQMF010000000
VERSION     DAOQMF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16749_length_3169_cov_3.336545"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLV77_02900"
     CDS             <1..432
                     /locus_tag="PLV77_02900"
                     /inference="COORDINATES: protein motif:HMM:NF036805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC14827.1"
                     /translation="DTPGHSSDHVCFMATPIPGGACFSGDLILGRGFTYVPPDGGSLA
                     SYMDSLKLLQTWQPALICPGHGPWITDPAAKISEYLEHRLMRENGLVEAMAAGERSRE
                     ALLDLVWSDVPEQLKSAARMVMEAHLQKLKAEGRLPDGLVK"
     gene            457..2406
                     /locus_tag="PLV77_02905"
     CDS             457..2406
                     /locus_tag="PLV77_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC14828.1"
                     /translation="MKESAPVELPKNLQLKELLRTIVGFGIPFLLITYLAFASGGYEI
                     VPRSQVGIIAWWSVLLGILAGILPLFRPTRASLAILAILCALLLWTACGLLWTESTEH
                     TIIELTRVATLLGVVVLLVVTQDREGLRNAVVAVAAAVVLVALVALVDRLLPNLLPFG
                     SDYTFPEGYPKARLNYPLEYWNGLGIFIAIGVGPVLWLVAAAKSTAVRCLAAGAVPLL
                     ALAGYMTASRGEIVEVAAVLAVLAVLFPERTRLLLNLIGPAIGSALLIFLINDRPAVR
                     DLDPGQARLDQGEQMALLVLAVVSAVALAQFAAFFFANRNEIKLGRPSRNAARNAGIA
                     AFAVVLVVAIGAFASGFAGDKWDEFKKPSELATVDRLSNVNSGERYLVWKSAVEASES
                     AKVTGIGPGTFEFWWARDGQGSQFVRDAHSIYLENLAELGPLGLILVLAMILGPIGAA
                     VLFSAREKDDRKRSLLAVSAAGMAAFAISAGIDWAWELTVLPVMFFTLAVAVAASTNP
                     IEFPERFRGSPWEVRIPMVIGSAAAMVLIWIPMKSAILIDGSQSAVRNGDLELALDKA
                     DKASDFEPWSTTALIQRAQVLRLLGRDEEAIGLAREAADKEPDNWTNWFVLSQLASDL
                     SPQQSRAARAKAKSLNPKSPIFTVE"
     gene            complement(2509..>3169)
                     /locus_tag="PLV77_02910"
     CDS             complement(2509..>3169)
                     /locus_tag="PLV77_02910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC14829.1"
                     /translation="SELEEKDTRSGAVPYELISHAVQEEIAIVEPRTEDLTPGVLRIG
                     FLGRNIPKKGIDLIIRAIGRNPERHWELAIAGPPGTDEFRDEMARLVRELEVGDRIRW
                     VGFLPERSDLFRACDVLAMPSAYEGFGMVAAEALCNGVPVIVPNRTGVAEIVKEFEAG
                     IVIPEAGVEELENALEVMEDDSTARSRFSENGLRAANSRLSYAAFAAATSKLYKSLIS
                     G"
BASE COUNT          599 a          911 c          954 g          705 t
ORIGIN      
        1 gacactcccg gccactcttc cgatcacgtt tgcttcatgg ccaccccgat ccccggcgga
       61 gcctgcttca gcggggatct gatcctcggc cgaggattca cgtatgtccc ccccgacggc
      121 ggttccctgg cttcgtacat ggattctcta aagcttctgc agacttggca accagctctg
      181 atctgtcccg gacacggacc ctggattacc gatccggcgg cgaaaatatc cgaatacctt
      241 gagcatcggt taatgagaga gaacggactt gtcgaggcaa tggccgctgg cgaaaggtca
      301 agagaagcgc tgctagatct cgtctggtcg gatgtgcctg aacaattgaa gtcagcagcg
      361 agaatggtta tggaggcgca tcttcagaaa ttgaaagcgg agggccgact gcccgatggc
      421 ctcgtgaaat agccagcgac ggacgagcta cctcagatga aagaatccgc gcctgtggaa
      481 ttgcccaaga accttcagct caaagaactg cttcgaacaa tcgtaggctt cgggattcct
      541 ttcctgttga taacctacct ggctttcgct tctggcggct acgaaattgt gccgcgaagt
      601 caggtcggga tcatagcttg gtggtcagtg ttgcttggga ttctggcggg gattctgccc
      661 ctatttcgcc cgacccgggc gagtctggcc attctggcaa ttctgtgtgc cctcctgctg
      721 tggaccgcct gcggtctgct ctggactgaa agtaccgaac acaccatcat tgaactgacc
      781 cgggttgcca cattgctcgg agttgtggtt ctactggtag ttacccagga tcgtgaggga
      841 ctgagaaacg cagtagtagc tgttgcggca gccgtggttc tggttgcttt ggtcgccttg
      901 gtcgatcggc tgctgccgaa cctgctcccg ttcggctccg actacacctt cccagaggga
      961 tacccgaagg cccggctcaa ctatccgctc gaatactgga acggattggg gattttcatt
     1021 gccattggcg tcggacccgt gctctggctc gttgcggcag caaagtcgac ggcggtgcga
     1081 tgtctggccg caggtgccgt tccgctgctg gccctcgccg gttatatgac cgcatcgcgt
     1141 ggcgaaatag tcgaggttgc agcggttctc gccgttctgg cggttctctt tcccgaacga
     1201 acaagactgc tcttgaacct gatcggcccg gcaatcggtt cggccttgct catctttctg
     1261 atcaacgaca ggccggcggt ccgtgatctc gacccggggc aggcccgcct agatcagggc
     1321 gagcagatgg cgttgctggt cctggcggtg gtttcagcgg tcgcgttggc tcagtttgcc
     1381 gcatttttct ttgccaacag gaacgagatc aaactaggcc gaccaagtcg gaacgctgca
     1441 cgtaacgcgg ggatcgccgc ctttgcggtg gttctggtcg tcgcgatcgg agcattcgcc
     1501 agcgggttcg cgggagacaa gtgggatgag ttcaagaagc cctccgagct ggcgacggtc
     1561 gaccggttgt ccaacgtcaa cagcggtgag cgctacctgg tctggaagtc agcggttgaa
     1621 gcaagcgaat cggcaaaggt gaccgggatt gggcccggga cctttgagtt ctggtgggcg
     1681 cgggacgggc agggctcgca gttcgttcgc gatgcccaca gtatttacct tgagaacctg
     1741 gccgaacttg gacctctcgg actgatcctg gttttggcga tgatcctggg accgatcgga
     1801 gccgcggttc ttttctctgc cagggagaaa gatgaccgaa aaaggtcatt gctcgccgtg
     1861 tccgcggcgg gcatggcggc ctttgcgatt tcagctggaa tcgactgggc ttgggaattg
     1921 accgttctgc cggtcatgtt cttcaccttg gccgtggcgg tcgctgcgtc aactaacccg
     1981 attgagttcc cggagagatt ccgcggatcc ccttgggagg tccggatacc gatggtcatt
     2041 gggtcagccg cggcgatggt actcatctgg ataccgatga agtcggccat cctgatagac
     2101 ggcagccagt cagcggtccg caacggggat ctcgagttgg cgctggacaa agctgacaag
     2161 gcatcggact tcgaaccttg gtcgaccacg gcgctgatcc agcgcgccca ggttctgcgg
     2221 ctcttaggcc gcgacgaaga ggcgatcggt ttggcccgag aagcggcgga taaggagcca
     2281 gacaactgga ccaactggtt cgtactttct cagctcgcgt ccgacctatc tccacagcaa
     2341 tctcgggcgg cccgggcaaa ggccaaatct ctaaatccga agtcgcccat cttcactgtc
     2401 gagtgagcga ctgtgggttt tgcgcgtggt tcagggccgg gtacggtgat cggtatggtc
     2461 ccgactgcct taacggcctg aacgggatat ccgtgtgagg ctcgtagctc aaccagaaat
     2521 caagctcttg tagagctttg atgtggctgc ggcaaaggct gcgtaagaaa gccgggagtt
     2581 ggcggccctg agaccgttct cggaaaacct gcttcgagcc gttgaatcgt cctccatgac
     2641 ttccagcgcg ttctcgagct cttcgacccc agcttccggt atcacaatcc cggcctcgaa
     2701 ttccttgact atctcggcaa ccccggtgcg gtttggcact atgaccggaa ccccattgca
     2761 gagagcctct gcggcaacca tcccgaaacc ttcgtacgcc gaaggcatgg ccagcacgtc
     2821 gcatgcgcga aagagatcag accgctccgg gaggaatccg acccacctga tccggtctcc
     2881 gacttctaat tcccttacca gccgagccat ctcatcgcga aactcgtcgg taccgggggg
     2941 gccggcaatc gcaagttccc aatgtcgctc cgggttcctg ccaatcgctc ggatgatgag
     3001 atcgatgccc ttctttggga tgtttcggcc gaggaaacct atcctcagaa caccaggggt
     3061 gaggtcttcc gttcgtggct cgacaattgc gatctcttcc tgcaccgcat gggagatcaa
     3121 ttcgtacggc accgcaccgg agcgtgtgtc tttctcttcg agttccgac
//
LOCUS       DAOQMF010000120         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16768_length_3167_cov_3.337082, whole genome shotgun sequence.
ACCESSION   DAOQMF010000120 DAOQMF010000000
VERSION     DAOQMF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16768_length_3167_cov_3.337082"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLV77_02915"
     CDS             complement(<1..528)
                     /locus_tag="PLV77_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNC14830.1"
                     /translation="MSTSLVILREQAGHETGSHPENSRRFPGVLNRLGEDSQRHGLPI
                     LESREATVEDLLRAHTESHVENVRVAAENGPTWLDGDTVASPLSYEVARRSAGAALVA
                     VDAVNGKAAEGTATTSSFAIIRPPGHHATADRAMGFCLFSNAAIAARYAREALGIGRV
                     AVLDWDVHHGNGTQDI"
     gene            complement(525..1406)
                     /locus_tag="PLV77_02920"
     CDS             complement(525..1406)
                     /locus_tag="PLV77_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC14831.1"
                     /translation="MSDSGEIAEGEELAVGAPDPADQAPALHSRENVRSVRVKAPTRG
                     NRKLEKFLDAVNRDDEVKAWWYMAQTHAERLGMSDHSWVHMQIVLNIALRLTRLLDKG
                     GVHGAMVTDHGMSARDTEVVVAGGALLHDVGMSIHRADHEEYSLFLAREALPRLLDGI
                     YEEPEKSIVMAEVLHAIIGHRRRGQPYTVEAGIVRVADALDMAEGRTRVPLESGQEGI
                     HSISAAAIDRVNISAGENRPVRLEIELNNSAGIFQVDDLLATKIRDTPLAGHIEVVAE
                     VKGESEKRLLSGFRLGA"
     gene            complement(1403..2215)
                     /locus_tag="PLV77_02925"
     CDS             complement(1403..2215)
                     /locus_tag="PLV77_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14832.1"
                     /translation="MSSGSTRRIVERSHKVTAGIDGLDRISALIMSEAEFARDQGAGR
                     VEVIAVPGLRGSRLIRLLDRVADAVGAGPIRIPTRRDWTAATFLGVTVPAGDSLPDPV
                     AVAVIGETAIGFAVGRPGEIPEWNGSRPVGASTMTRKARFQDPPRPGQIEAAVIGASR
                     GIASMFPPPTERVLVVSSLAPVVERLCGPRIGAAEARRGLDAILGQTSDDISAWFGAE
                     APLARHLPGIIVGHAALAEGLGVRVEPALCDLAASRYWLAEREEPVAGRESS"
     gene            complement(2348..2743)
                     /locus_tag="PLV77_02930"
     CDS             complement(2348..2743)
                     /locus_tag="PLV77_02930"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HNC14833.1"
                     /translation="MTSETPVGVAFQIGDLVQQSRAEAAERIVERQQAEVQRKLEARQ
                     QQEKFGDLPGSVTMETLEAIADCESGGDPKIVSSSGLYFGKYQFSPGTWESVGGKGLP
                     SDAPEAEQDYRAALLYERSGPGQWPVCGQ"
     gene            2918..>3167
                     /locus_tag="PLV77_02935"
     CDS             2918..>3167
                     /locus_tag="PLV77_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14834.1"
                     /translation="MPRSGWQLLRDGFPDRPEMGPALSRTLLEQVAAGQRPATVRLSR
                     PGRVVAFGRRDVVSPGYADAVAAARAGGFPGMERLSGGR"
BASE COUNT          500 a         1076 c         1067 g          524 t
ORIGIN      
        1 gatgtcctgg gtgccgttgc cgtggtggac gtcccaatcg agcaccgcga cccggccaat
       61 tccgagggcc tcgcgggcat agcgggcggc gatcgcggcg ttgctgaaga gacagaagcc
      121 catcgcccgg tcggcggttg cgtgatggcc gggcggacgg atgatcgcga aggaactggt
      181 ggtcgcggtc ccttcggccg ccttgccgtt cacggcatcc acggcgacca gcgcggctcc
      241 ggccgaacgc cgggccacct cgtagctcag gggcgacgcg accgtgtcac cgtccagcca
      301 ggtcggaccg ttctcggccg cgacccgcac gttctccaca tgagactcgg tgtgggccct
      361 cagcaggtcc tcgaccgtgg cttcccggga ttcgaggatc ggcaggccgt gccgctggct
      421 gtcttcgccg agacggttca gaacccccgg gaaacggcgt gagttttccg gatggctccc
      481 ggtttcatgc ccggcctgct cgcgcagaat gaccagactg gtgctcatgc gccgagccgg
      541 aaaccggaaa ggagccgctt ctcgctttcc cccttcactt cggccacgac ctcgatgtgg
      601 ccggccaggg gcgtgtcgcg aatcttggtc gcgagcaggt cgtcgacctg gaatatcccg
      661 gccgagttgt tgagttcgat ctcgagccgg accgggcggt tctccccggc cgaaatgttg
      721 acccggtcga tcgccgcggc ggaaatcgag tgaatccctt cctggccgga ttcgagcggc
      781 acccgggtcc ggccctcggc catgtcgagc gcatcggcca cccggacgat tccggcttcc
      841 accgtgtatg gctgcccgcg ccgccggtga ccgatgatcg cgtgaagcac ctcggccatg
      901 acgatcgact tctcgggttc ctcatagatc ccgtcgagca gccgcggcag tgcctcgcga
      961 gccagaaaaa ggctgtattc ctcgtggtcg gcccggtgga tcgacatgcc gacgtcatgg
     1021 agcagggctc cgcccgccac caccacttcg gtgtcacggg cgctcatccc gtggtcggtg
     1081 accatcgctc catgcacccc gcccttgtcg agcagccggg tcaggcgcag ggcgatgttg
     1141 agcactatct gcatgtggac ccaggagtgg tcggacatgc cgagccgctc ggcatgggtc
     1201 tgggccatgt accaccaggc cttgacctcg tcatcccggt tgacggcgtc gaggaacttc
     1261 tccagcttgc ggttgccgcg ggtcggcgcc ttgacccgga ccgacctgac gttctcgcgg
     1321 ctgtgcaacg ccggggcctg atcagccgga tcgggtgcgc cgaccgccag ctcctcccct
     1381 tctgcgatct cccccgaatc gctcaagacg attcccggcc agcgaccggt tcctcacgct
     1441 cggccagcca gtagcggctt gccgcgaggt cgcaaagggc aggctcgacc cggactccga
     1501 gcccttcggc cagtgccgcg tgaccgacga tgatgcctgg cagatgccgg gccagggggg
     1561 cttcggcgcc gaaccaggcg gaaatgtcat cgcttgtctg gccgagaatc gcgtccagtc
     1621 cccggcgggc ctccgcggcg ccgatccgcg ggccgcaaag tcgctcgacc acgggggcga
     1681 ggctcgagac gaccagcacc cgctcggtcg ggggcgggaa catcgaggcg atcccgcggg
     1741 aggcgccgat cacggccgct tcgatctggc cgggccgggg cggatcctga aaccgggcct
     1801 tgcgggtcat ggtcgaggcg ccgaccggcc gtgagccgtt ccattccggg atctctcccg
     1861 ggcggccgac cgcgaagccg attgccgtct ccccgatcac ggcgacggcg accgggtccg
     1921 gcaacgagtc cccggcggga accgtcacac cgaggaaggt cgccgctgtc cagtcccggc
     1981 gggtggggat ccggatcggc ccggctccga cggcgtcggc aacccggtcg agcaaacgaa
     2041 tcaggcggga accgcggaga ccgggaaccg cgatcacctc gaccctcccc gccccctggt
     2101 cccgggcgaa ctccgcctcg gacatgatca gggccgagat ccggtcgaga ccgtcgattc
     2161 cggccgtgac cttgtggctg cgctcgacga tcctccgggt tgaaccggaa gacacttccg
     2221 acagcgaaaa gcgggtactg cggagcgaga tttcaattgc cccaaggatc aagaccgggc
     2281 catcctagag gcaatgtgcc cgaaagccga attaatcctg taaacgccgg tattggacgg
     2341 aaagccgcta ctggccgcag accggccact ggccgggccc ggagcgctcg tacaggagcg
     2401 cggcacggta atcctgctcg gcctccggag catcagacgg gagacccttg ccaccgaccg
     2461 attcccaggt gccgggactg aactggtact tgccgaagta gaggccgctg gaggaaacga
     2521 tcttcggatc accgcccgac tcgcaatcgg cgatcgcttc gagagtctcc atggtgaccg
     2581 accccgggag gtccccgaat ttttcctgct gctgcctggc ttcgagtttc cgctgtacct
     2641 cggcctgctg cctttcgacg atccgttcag ccgcttcggc cctggactgc tggaccaggt
     2701 caccgatctg gaaggcgacg ccgaccggcg tttccgaggt caccgcggca agggcgatgt
     2761 cggcaccgga agtcccggag ccgtccttcg accggtcgaa ggcgaggttc ccggcgataa
     2821 tcagggcagc gatcagcgcc agaatgagga ttgaatttcg cgtcgtaagg atgatttctc
     2881 ccttttgtta gcaaacactc atatcagaag cgaccggatg ccgcgttccg gatggcagct
     2941 gttgcgcgac ggtttcccgg atcggccgga aatggggccg gccctttccc ggaccctgct
     3001 ggagcaggtg gcagccggcc agcgcccggc cacggtcagg ttgagccggc ccgggcgggt
     3061 ggtcgccttc ggccggcggg acgtggtctc ccccggatat gcggatgccg tcgccgccgc
     3121 cagggcgggc gggttccccg gcatggaaag gctgtccggt ggccggg
//
LOCUS       DAOQMF010000121         3165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16792_length_3165_cov_3.125080, whole genome shotgun sequence.
ACCESSION   DAOQMF010000121 DAOQMF010000000
VERSION     DAOQMF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16792_length_3165_cov_3.125080"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLV77_02940"
     CDS             <1..747
                     /locus_tag="PLV77_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC14835.1"
                     /translation="GTTRLHRGDERIRHFMGTSTFAEYTVMPEIALAKINPEAPLDHA
                     CLFACGLSTGLGAAMFTAKVEPGTTCVVFGAGMVGLGAVAGARLMGAERIICVDLSDD
                     RLELARGQGATDLHKADENTVQWILDETGGMGADYTFEATGLVGVMQQAVEAARMAWG
                     LCTIAGVAGKGETLDIIPRWLITGRRVAGSSFGGVKGRDQVPQLVDRYLAGEIDVEPF
                     LSHRISLDEVNKGFDLMEAQDGIRSVIEFT"
     gene            827..1576
                     /locus_tag="PLV77_02945"
     CDS             827..1576
                     /locus_tag="PLV77_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC14836.1"
                     /translation="MIIQRVEQSEWLSNSYLVCDETGGSGVLVDGNGVIEPLLERVDR
                     EGITLTHILLTHEHWDHVVDLREVADRYGVPIVASQKTADLVDFKVDQIVEDGDETVS
                     GGLTIRWIATPGHSDGHMALLINGTDVITADVIFKGTVGGTVAPGETGFPELKSSIMD
                     RLMTLPPETRIHPGHREPSTVGEEWENNPFIRVWRGLDEEGDDPCEVNNFGPATLILW
                     APDYDGTNKAWIRLPDGEDKITGGSQILSRG"
     gene            complement(1586..2776)
                     /locus_tag="PLV77_02950"
     CDS             complement(1586..2776)
                     /locus_tag="PLV77_02950"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNC14837.1"
                     /translation="MSQVDPSRTSTSGRILAFGAALLATVVGSMSLAAQSPAAIQEGI
                     VEVNGYAWTVNPVAVDLNDIAPTNNGSSRYTVLEILEEGQNGQYRISQIPRVTINLPG
                     KLTGPGCSGDQIRGNSNQCPTFRSTPDATIMSYKAGSQTRQVEYRDYNPQIYIEKADD
                     MSVDLSPPSAKIDSGGQVRFTTDVSGDIGAVTYEWDFGDGTKKTTSKGSISHTFKGSN
                     RTYTVVVSVTSTGNPRSADDLSTITVGKVKKKPADQNPNQKNPDNSDNGGGGETTEST
                     PYVPGYSGGSTGGTGGGSPGASPNTSPGQPKREKQQPANESSGQTVTGQLIDPASIAT
                     ATPSTGDSTAATEQASPDDGSGGGGIPDGAKAALGIGALLGLGGLAEAGAFTGAFRRI
                     RFRL"
     gene            complement(2773..>3165)
                     /locus_tag="PLV77_02955"
     CDS             complement(2773..>3165)
                     /locus_tag="PLV77_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14838.1"
                     /translation="RGTGLVGFRYCHNTINFTAASPVIEIDGDANSRLIFTVHGTDGT
                     AFPEQPAVMVKLIPSRAQSHTVVDNMNGYSTVTWEKIPGYVPAEGTGIFAGFYPAFSP
                     DYEGQPQTERPDRFGYFSITYTFPNTDH"
BASE COUNT          508 a         1026 c         1061 g          570 t
ORIGIN      
        1 ggcaccacgc gcctgcaccg cggcgatgag cggatccgcc acttcatggg cacctcgacc
       61 tttgccgagt acacggtgat gccggagatc gccctggcca agatcaatcc cgaagctccg
      121 cttgaccatg cctgcctttt cgcctgcggc ctttcaaccg ggctgggcgc ggcgatgttc
      181 acggccaagg tcgaaccggg cacgacctgc gtcgtcttcg gagccggcat ggtcggcctc
      241 ggcgcggtcg ccggggcgcg cctgatgggg gcagagcgga tcatctgtgt cgacctctcg
      301 gatgaccggc tcgaactggc ccggggccag ggcgcgaccg acctccacaa ggcagacgaa
      361 aacacggtcc agtggatcct cgacgaaacc ggcggaatgg gcgccgacta caccttcgaa
      421 gcgaccggcc tggtaggggt gatgcagcag gcggtcgaag cggcccgcat ggcatggggg
      481 ctctgcacga tcgccggcgt ggccggcaag ggcgagactc tcgacatcat tccccgctgg
      541 ctgatcaccg gtcgccgggt cgccggctct tcgttcggcg gcgtcaaggg ccgcgaccag
      601 gtgccgcaac tggtcgatcg ctacctcgcc ggcgagatcg acgtcgaacc gttcctctcc
      661 caccggatca gcctcgacga ggtcaacaag ggctttgacc tgatggaagc ccaggacggc
      721 atccgcagcg tgatcgagtt cacctgatgc cccgcccgca gccgatgccg cgctcccggc
      781 cgaaaaccag tcactcttcc ttccatccca cccgaaagga ccgcgcatga tcatccagcg
      841 agtcgaacag tccgaatggc tctccaactc ctacctcgtc tgcgacgaga ccggcggttc
      901 gggggttctg gtcgacggca acggagtgat cgagcctctg ctggagcggg tcgaccggga
      961 agggatcacc ctcacccaca tcctgctcac ccatgaacac tgggaccacg tggtcgacct
     1021 gcgcgaggtc gccgaccgtt acggggtgcc gatcgtggcc agccagaaga ccgccgacct
     1081 ggtcgacttc aaggttgacc agatcgtcga ggacggggac gaaaccgtct cgggcggact
     1141 gacgatcagg tggatcgcca ccccgggaca ttcggacggc cacatggccc tgctgatcaa
     1201 tggcaccgac gtgatcaccg ccgacgtgat cttcaagggc acggtcggcg gcacggttgc
     1261 cccgggcgag accgggtttc cggagctcaa gagctcgatc atggaccggc tgatgaccct
     1321 gccgcccgag acccgcatcc atcccggcca ccgcgaacca tcgacggtcg gcgaggaatg
     1381 ggaaaacaac cccttcatcc gggtctggcg cggtctcgac gaagagggcg acgatccctg
     1441 cgaggtcaac aacttcggcc cggcgaccct gatcctctgg gcgcccgact acgacggcac
     1501 caacaaggcc tggatccgcc tgccggacgg cgaagacaag atcaccggcg gttcgcagat
     1561 cctcagccgc ggctgagacg acgagctaga gccggaagcg gatacggcgg aaggccccgg
     1621 tgaaggcgcc ggcttcggcc agcccgccga ggccgagcag ggcgccgatc ccgagcgcag
     1681 ccttggcgcc gtccgggatg cccccgccgc ccgagccatc gtccgggctc gcttgctcgg
     1741 tcgccgcggt cgagtcaccg gtcgaagggg tcgcggtggc gatgctggcc ggatcgatca
     1801 actgcccggt cacggtctgg ccgctgcttt cgttcgccgg ctgctgcttt tcccgcttcg
     1861 gctgaccggg gctggtgttc ggcgaagccc cggggctgcc gccgccggtt ccgcccgtgc
     1921 tgccgcccga atacccggga acgtatgggg tcgattcggt ggtctcccct ccgcccccgt
     1981 tgtcgctgtt gtccgggttc ttctggttcg gattctggtc ggccggcttc ttcttcactt
     2041 tgccgacggt gatcgtggac aggtcatcgg cgctgcgggg gttgccggtc gaagtgacgc
     2101 tgaccacgac cgtgtaagtc cggttgctgc ccttgaaggt gtgggagatc gagcctttcg
     2161 aagtcgtctt tttggtcccg tcgccgaagt cccactcata ggtgacggcg ccaatgtccc
     2221 cgctgacatc ggtcgtgaac ctgacctggc cgccgctgtc gatcttggcg ctgggcggcg
     2281 aaaggtcgac cgacatgtcg tccgctttct cgatgtagat ctgcgggttg tagtcccggt
     2341 actcgacctg cctggtctgc gagccggcct tgtagctcat gatggttgcg tcaggggtgg
     2401 accggaaggt cgggcactga ttcgagttgc cgcggatctg gtcgccggag cagccgggtc
     2461 cggtcagctt tccggggaga ttgatcgtga cacgggggat ctgactgatc cggtactggc
     2521 cgttttgccc ttcctcgagg atctcgagga ccgtgtacct gctgctcccg ttgttggtcg
     2581 gtgcgatgtc attcaggtcg accgcgaccg ggttcacggt ccaggcgtat ccgttcactt
     2641 cgacgatccc ctcctggatc gcggccgggg attgagcagc cagggacatc gagccgacga
     2701 ctgtcgccag cagggccgcc ccgaaggcga ggatccgccc cgaagtcgat gtccggctcg
     2761 gatcgacctg gctcaatggt ccgtgttcgg gaaggtgtag gtgatcgaga agtaaccgaa
     2821 ccggtcgggg cgttcggtct ggggctggcc ttcgtagtcc ggactgaagg ccggatagaa
     2881 gccggcgaaa atgcccgtgc cctcggccgg cacgtagccg gggatcttct cccaggtcac
     2941 ggtgctgtaa ccgttcatgt tgtcgaccac cgtgtgtgac tgggcccggc tcgggatcaa
     3001 cttgaccatg acggccggct gctcggggaa ggcggtcccg tcggttccgt ggacggtgaa
     3061 gatcagtcgg gagttggcgt caccatcgat ttcgatcacc ggactggcgg cggtgaagtt
     3121 gatcgtgttg tggcagtagc ggaaaccgac caggccggtt cccct
//
LOCUS       DAOQMF010000122         3164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16799_length_3164_cov_3.669990, whole genome shotgun sequence.
ACCESSION   DAOQMF010000122 DAOQMF010000000
VERSION     DAOQMF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16799_length_3164_cov_3.669990"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLV77_02960"
     CDS             complement(<1..785)
                     /locus_tag="PLV77_02960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC14839.1"
                     /translation="MDSGFEARLARRREEVRRRQIVRRRVVLGSMLGVLLAAAVFMLF
                     LRSDDSGESGTQQTAGNNTAGQQGAGTSETSQTSVGTEASRGGAQPDDSWGPHTGPVP
                     ILMYHVIGPAAGSEDYPGLFLSTPDFQDQVDWLADNGYTAVTLVQVQNAWYDGGTLPE
                     RPVVLSFDDGYLGQYLFAMPILEKQGWGGQLNLKSEGSDLSSKQVKKMYRAGWEIASH
                     SITHPDLTTLDPATLEHELVGSKEELEKDLGFEIVNFCYPAGQ"
     gene            complement(789..2180)
                     /locus_tag="PLV77_02965"
     CDS             complement(789..2180)
                     /locus_tag="PLV77_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC14840.1"
                     /translation="MTNTPINSPNGSGKPPWYRQRGKVISLIAVVLVAAGAIALVAWN
                     ELKRPDDVSNPDVPFVKTKPKQVVPKDRTINWSTFRLNPQRTGYLPVNGIHPPFKRVW
                     KYGDQPLLEFPPIVVDGRLYFVDNDGNARALRADNGREIWHRKIADLNASTPTYARGR
                     LYIVNLEPGHVLSMNAKNGRIIWEKPLPCRSESSPAVIRQRVYFGCENGVLYALKARN
                     GKEVWETSVDGAIKGAPAYEDGTLFVGDYGGVVSAVRAKTGEIKWQSDSLGGSFGRAG
                     SFYSTPAVAYGRVYLGSNDGRVYSFDKNTGELAWTYSTGDWVYSGPSVAQVPGTPPTV
                     YIGSFDGNVYALNARSGEARWTFPMGGQVIGSLSVIGRTVYAATFDGTTTYGISAKSG
                     RKVFEFHTGAYMPGISDGEKLYLVGYSSIHAMKPITLKQLRAERRAKEARKAARQKAR
                     KQAAQNQAGPSGQ"
     gene            2214..>3164
                     /locus_tag="PLV77_02970"
     CDS             2214..>3164
                     /locus_tag="PLV77_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14841.1"
                     /translation="MTPGIDPDFNRLATLIRTHPAIAPIREAATTPLYLVGGAIRDAL
                     ADFRVDDIDLVIEGDPVPLAAGFDPAAKVNDRFGTVNLLVDGYPVDIATARTETYSRP
                     GALPDVRPGTLEDDLRRRDFTINAMAIGIDEGSEMIDQFGGIADLESGVLRVLHDASF
                     EDDPTRALRAARYAARLDFDLEPKTAELMMKVNLGSISRDRVESELRLIALEDNALEA
                     LRLARLWGLVDFPEERLDLAEQAIELIEIEPWRDEAPREALILEVVFGNLSGAEELLG
                     EPGTPWQACRLTRGRTALELVVARALGAVWLDRWQREWRWV"
BASE COUNT          510 a         1009 c         1082 g          563 t
ORIGIN      
        1 tactggccgg ccgggtaaca gaagttgacg atctcgaagc ccaggtcctt ctcgagttcc
       61 tccttcgagc cgaccagttc gtgttcgagg gtggcgggat cgagggtggt gagatcgggg
      121 tgggtgatcg agtgggaggc gatctcccag ccggcccggt acatcttctt cacctgcttc
      181 gaggagaggt ccgagccctc cgacttgagg ttgagctggc cgccccagcc ctgcttctcg
      241 aggatcggca tcgcgaacag gtactggccg aggtagccgt cgtcgaagga aaggacgacc
      301 ggcctctccg gcagggtgcc gccgtcatac caggcgttct ggacctggac gagggtgacc
      361 gcggtgtagc cgttgtcggc cagccagtcg acctgatcct ggaaatcggg tgtggagaga
      421 aagagaccgg ggtagtcctc ggatccggct gcggggccga tcacgtggta catcaggatc
      481 ggcaccgggc cggtgtgcgg cccccagctg tcgtcgggtt gggcgccgcc ccgtgatgct
      541 tcggttccaa cgctggtctg cgacgtctcg gaagtcccgg cgccttgctg tccggcggtg
      601 ttgttgccgg cggtctgttg agtgccgctc tcgcccgagt cgtcggaacg caggaagagc
      661 atgaaaaccg ccgccgcgag cagcaccccc agcatcgacc cgagcacgac gcgacggcgg
      721 acgatctgtc ggcgacggac ctcctcccgc cggcgggcca gccgggcctc gaaaccggag
      781 tccaccggct actgcccgct tggcccggcc tgattctggg ctgcctgctt gcgggccttc
      841 tggcgggcgg ccttgcgggc ttccttggcg cggcgttcgg cccgcagctg cttgagcgtg
      901 atcggcttca tcgcatgaat gctcgagtag ccgaccaggt agagcttctc gccatccgag
      961 atccccggca tgtaggcgcc ggtgtggaac tcgaagacct tgcgtccgga cttggcgctg
     1021 atcccgtagg tggtggtgcc atcaaaggtc gcggcgtaga ccgttcggcc gattacgctg
     1081 agcgaaccga tcacctggcc gcccatcggg aaggtccagc gtgcctcgcc ggaccgggcg
     1141 ttgagggcgt agacgttgcc gtcgaaggag ccgatgtaga cggttggcgg agtgcccgga
     1201 acctgggcga ccgacgggcc cgagtagacc cagtcgccgg tcgagtaggt ccaggcaagt
     1261 tcgccggtgt tcttgtcaaa gctgtagacc cggccgtcgt tgctgccgag atatacgcgg
     1321 ccgtaggcga ccgccggtgt cgaatagaag gaaccggccc ggccgaagct gccgccgagg
     1381 ctgtccgact gccacttgat ttcaccggtc tttgcccgca ccgccgaaac gacgcctccg
     1441 tagtcgccca cgaagagggt gccgtcctca taggccggtg ctcccttgat cgccccgtcg
     1501 accgaggttt cccacacttc cttgccgttt cgggccttga gcgcatacag caccccgttc
     1561 tcgcaaccga agtacacccg ctggcgaatc accgcgggcg aggattcgga acggcagggc
     1621 aaaggcttct cccagatgat ccggccgttc ttcgcgttca tcgaaagcac gtggccgggc
     1681 tcgagattca cgatgtaaag gcggcccctg gcataggtcg gggtcgaggc attcaggtcg
     1741 gcgatctttc gatgccagat ctcccgtccg ttgtcggccc gcaaggctct cgcgttaccg
     1801 tcgttgtcga cgaagtaaag ccggccatcg accacgatcg gcgggaactc gagcagcggc
     1861 tggtcgccgt acttccagac ccgcttgaag ggcggatgga tgccgttcac cggcagatag
     1921 ccggtgcgct gggggttgag gcggaaagtg gaccagttga tcgtcctgtc cttcggcacg
     1981 acctgcttcg gcttggtctt gacgaacggg acgtcgggat tgctgacgtc atcgggccgc
     2041 ttcagctcat tccaggcgac aagggcgatc gcgccggcgg ccaccaggac caccgcgatc
     2101 agcgaaatga ccttgccgcg ctgccggtac caggggggct tgcccgaccc gtttggagag
     2161 ttgattggtg tgttggtcat ccgaagaagg gatcatggta cgggagtgac ccgatgaccc
     2221 ccggaattga ccctgacttc aaccgactcg ccaccctgat ccggacccac ccggcgatcg
     2281 cgccgatccg tgaagcggcg accacgccgt tgtacctggt ggggggtgcg attcgtgacg
     2341 ccctcgccga tttccgggtc gatgacatcg atctggtcat cgaaggggac ccggtgccgc
     2401 tggccgccgg gtttgacccc gcggcgaagg tcaacgaccg tttcggcacc gtcaacctgc
     2461 tggtcgacgg gtatccggtt gacatcgcca ccgcccgaac cgagacctac tcgcgtcccg
     2521 gagcgcttcc ggacgtccgc ccgggcaccc tcgaggacga cctgcgccgc cgcgacttca
     2581 cgatcaacgc gatggcgatc ggcatcgacg agggctcgga gatgatcgat cagttcggcg
     2641 gcatcgccga cctggagtca ggggtgctca gggtgctcca cgacgcatcc ttcgaggatg
     2701 acccgacccg ggcgctccgg gcggcccgct acgccgcccg cctggacttc gacctcgaac
     2761 cgaagacggc cgagctgatg atgaaggtga acctcggttc gatctcacgg gaccgggtcg
     2821 aaagtgaact gcgcctgatc gccctcgagg acaatgccct ggaagcgctg cggctggccc
     2881 ggctctgggg acttgtcgac tttccggagg agcggctcga cctcgcagaa caggcgatcg
     2941 agttgatcga gatcgaaccc tggcgggatg aagccccgcg ggaggcgctg atcctcgagg
     3001 tcgttttcgg caacctgagc ggggccgagg agctccttgg ggagcccgga actccctggc
     3061 aggcctgccg gctcacccgg gggcggaccg cgctggaact tgtcgtggcc cgcgcgctgg
     3121 gcgccgtctg gctggaccgt tggcagcgcg aatggcgctg ggtg
//
LOCUS       DAOQMF010000123         3152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16914_length_3152_cov_3.331611, whole genome shotgun sequence.
ACCESSION   DAOQMF010000123 DAOQMF010000000
VERSION     DAOQMF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16914_length_3152_cov_3.331611"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLV77_02975"
     CDS             <1..967
                     /locus_tag="PLV77_02975"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14842.1"
                     /translation="AGIYASPAQIRRCELRSGDEVSGPVRPARRGERRPSLVRVEKVN
                     GEPPVDERSPRFESLTPVAPHRHMPLSGDADDILARSVDLLAPLAFGQRVLVEAQPRS
                     GRTSFLRSLAKAIARGAGDEAEIIVLLVDERPEEVTAWSREVPEVEIAAAPADLGAHE
                     QVRTAERILAKVKRQAEAGNDVVIIVDSLTRLGNAYGEAGAVKPFFGAGRELEEEGAG
                     SVTVIATALWGSPGDQDVMDAVHTTENATIRLDASLASAGVEPSLDVTACAVSGQESV
                     LDQEQVDAVRRLRAELKDLEPVPAANRLAELIKSSPSNEELLKNL"
     gene            complement(1058..1552)
                     /locus_tag="PLV77_02980"
     CDS             complement(1058..1552)
                     /locus_tag="PLV77_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14843.1"
                     /translation="MPRVLKASTVESKKTRRGNYESGTDYVLEYGNLRFSFNERDFSQ
                     RVEQAAVRLGFVTPGLTFAELNDLLHLAVSGEIEEPSSELGIHVTHHWEDLTGPGNQS
                     FVHWLRRLVFRGAWLDQRVKEGELDIAFDEGRQTFGYIQPDRGPEMIELAKEPSWRRV
                     AFRR"
     gene            1789..2130
                     /locus_tag="PLV77_02985"
     CDS             1789..2130
                     /locus_tag="PLV77_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNC14844.1"
                     /translation="MAGDPVSRLLGLALLDRHRAPEGLLIPRCRSVHTYGMRFPLDLL
                     FLGPDCQPLIAVREVGPGHRVRVPEAAAVLELPSDWDLEKWEGATGAGSGGEPGTAEP
                     DGPGDPGQGRG"
     gene            2130..2840
                     /locus_tag="PLV77_02990"
     CDS             2130..2840
                     /locus_tag="PLV77_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC14845.1"
                     /translation="MLPVREGIARIVVCEDDQPTLELLCEHLTADRFSVLAAPSAADA
                     MRLCLYNDPDLMLLDLGLPDAAGLDLLREIRNPDRTVNRVDPGIGVIIVSGRSTERDR
                     VRGLSEGADDYVQKPFSYGELLARINSVLRRRRRSHSLVQVGEIEINDVEHTVRVGGR
                     YLRLSMKEYALLKILANEPSRVFSKEELLQEVWGYRSSGHTRTLEAHCSRLRKKLDPK
                     HGRYVVNCWGVGYRLVGP"
     gene            2852..>3152
                     /locus_tag="PLV77_02995"
     CDS             2852..>3152
                     /locus_tag="PLV77_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14846.1"
                     /translation="MSIGLADLIPLLAALFVLAGREAWLAHRRVLVNRALHEARRPLQ
                     AIALSLPAGPGRMTPVLPVWQAIQALGEVDRQINGGAPDRPRPEPKRCWPNWSSTA"
BASE COUNT          479 a         1036 c         1119 g          518 t
ORIGIN      
        1 ggccgggatc tacgcctcgc ctgcccagat ccgccgttgc gaactccgct ccggcgacga
       61 agtcagcggt ccggtccgcc cggcccgccg tggcgagcgc cggccttcac tggtccgggt
      121 cgagaaggtc aacggcgagc cgccggtgga cgagcgctcc ccccgcttcg agagtctgac
      181 ccccgttgct ccgcatcggc acatgccgtt gagcggcgac gccgatgaca tcctcgcccg
      241 ctcggttgac ctgctcgctc cgctggcctt cggccagcgg gtgctggtcg aggcccagcc
      301 gcgttccggc cggaccagct tcctgcggtc gctggccaag gcgatcgccc gcggtgccgg
      361 tgacgaagcc gagatcatcg tgctcctggt cgacgagcgg ccggaagagg tgaccgcctg
      421 gtcccgcgag gtgccggagg tcgagatcgc cgccgcgccc gccgacctcg gtgcccacga
      481 gcaggtccgg accgccgagc ggattctggc caaggtcaag cgccaggccg aggccggcaa
      541 cgacgtcgtg atcatcgtcg actcgctgac ccggctcggc aacgcctacg gcgaggccgg
      601 agcggtcaag cccttcttcg gggccggtcg cgaactggaa gaggaaggtg ccggctcggt
      661 caccgtgatc gccaccgccc tctggggctc gcccggcgac caggacgtga tggacgcggt
      721 ccacacgacc gagaacgcaa ccatccgtct cgatgcctcg ctggcttcgg ccggggtgga
      781 gccgtcactg gacgtcaccg cctgcgccgt ttccggtcag gagtcggtac tggaccagga
      841 acaggtggac gcggtgcgac gcctccgggc tgaactgaag gacctggaac cggtcccggc
      901 cgccaatcgc ctggccgagc tgatcaagag ctccccaagc aacgaagaac tgctgaagaa
      961 cctgtagcta gagcccctcc caaccatcgt tttgtgcgaa tgggaggggc gggtccagaa
     1021 tggagggcgg gtcggacccc gcccgacttg aagttctcta tcggcggaag gcgacgcggc
     1081 gccagctcgg ctctttggcc agctcgatca tctcggggcc gcggtcgggc tgaatgtagc
     1141 cgaaggtctg gcggccctcg tcgaaggcga tgtccagttc gccttccttg acccgctggt
     1201 cgagccaggc gccgcggaag acgagccggc gcagccagtg gacgaaactc tggttgccgg
     1261 gtccggtcag gtcctcccag tgatgggtga cgtggatgcc caactccgag ctcggctcct
     1321 cgatctcgcc ggaaacggcc agatggagca ggtcattgag ctcggcgaag gtcaggccgg
     1381 gagtcacgaa accgagccgc actgcggcct gttcgactct ctggctgaag tcccgttcgt
     1441 tgaacgagaa cctgagattg ccgtactcga gcacatagtc agtgcccgac tcgtagtttc
     1501 ctctgcgggt ctttttactt tccacggttg aagccttgag cacccgcggc atggtccttg
     1561 ggtgcccggg catcatgcct tgaggctgcc tttagggact tggcaccgcc ggacctgaac
     1621 gggcggttgc accccgaaag actcgttgca gagccgatcc ggttctgcgg gggagagttt
     1681 cctgtgcccg tgccttagag gggcatggcg attggtgcgg atatcacgcg gccggcgagg
     1741 cgtttccggc gacttgagcg gcggagggtt cacggtttcg aggtgatggt ggccggtgac
     1801 ccggtgtcgc ggcttctggg cctggcgctg ctggaccggc accgggcgcc ggaaggtctg
     1861 ctgattcccc ggtgccgttc ggtccacacc tacggcatgc ggtttccgct ggacctgctc
     1921 tttctcgggc cggactgcca gccgctgatc gcggtgcggg aggtcgggcc gggccaccgg
     1981 gtgcgggtgc cggaagccgc ggcggtgctg gaacttccct cggactggga cctggagaag
     2041 tgggaggggg caaccggcgc gggatctggc ggagagccgg gtaccgccga gccggacggg
     2101 cccggcgacc cggggcaggg gcggggctga tgcttccggt gcgggaaggg atcgcccgga
     2161 tcgtcgtctg cgaggacgac cagccgaccc tcgaactgct ctgcgaacac ctgaccgccg
     2221 accgtttctc ggtcctggct gcgccgagcg ccgccgatgc gatgcggctc tgcctctaca
     2281 acgacccgga cctgatgctg ctcgaccttg gtttgcccga cgcggccggg ctcgacctgc
     2341 tgcgcgagat ccgcaatccg gaccggaccg tgaaccgggt cgatccggga atcggggtga
     2401 tcatcgtcag cggtcgcagc accgagcgcg accgggtgcg cggcctcagc gaaggggcgg
     2461 acgattacgt ccagaagccc ttcagctacg gcgaactgct ggcccggatc aattcggtgc
     2521 tgcggcgccg gcggcgcagc cattccctcg ttcaggtcgg ggaaatcgag atcaacgacg
     2581 tcgagcacac ggtccgggtg ggtggccgct acctgcgact ttcgatgaag gagtacgcgc
     2641 tgctgaagat cctcgccaac gaaccgagcc gggtcttctc caaggaggaa ctgctgcagg
     2701 aggtctgggg ataccgctcc tccgggcaca cccggaccct cgaagcgcac tgcagccggc
     2761 tgcggaagaa gctcgatccg aagcacggcc gttacgtggt caactgctgg ggagtcggct
     2821 accggctcgt gggtccgtga ggggtgctga agtgagtatt ggacttgccg acctgatccc
     2881 cctgctggcc gcgctctttg tacttgccgg gcgggaagcc tggctggccc atcgccgcgt
     2941 cctcgtcaat cgggcgctcc atgaagcccg gcggccgctc caggcgatcg cgctctccct
     3001 gccggcgggg ccgggccgga tgaccccggt gctgccggtc tggcaggcaa tccaggcgct
     3061 gggcgaggtc gatcggcaga tcaacggcgg tgcacccgac cggccgaggc ccgagccgaa
     3121 gcgctgctgg ccgaactgga gctcgacggc gg
//
LOCUS       DAOQMF010000124         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16983_length_3144_cov_3.848818, whole genome shotgun sequence.
ACCESSION   DAOQMF010000124 DAOQMF010000000
VERSION     DAOQMF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16983_length_3144_cov_3.848818"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLV77_03000"
     CDS             <1..833
                     /locus_tag="PLV77_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14847.1"
                     /translation="NQFWSPNSQDVLLQERTRTIPATTLSEHVQQKGLTPNRIAIAHI
                     ERPAGKKLKVVSSEVGNWAVAPTQYFPSVGSNTSVTVNGKAGGTADLVYTGNLQTGSW
                     SSEYHDYTKDGKTFVNGTYFVSRTIEGVWTLKADVTVTGQHTGRLNMDMTIQGGDPLP
                     IMTGGITAVYDGKKAPALPKLGACYKKLPQKSRLAAQVKSKRAGKATKVTVTVTANVY
                     GDRRPVQNAKVKLAGRIVRTDRSGKAKLKVRGRGTRKVLVTAGDTFIKTSKVAHLKQR
                     "
     gene            935..2095
                     /locus_tag="PLV77_03005"
     CDS             935..2095
                     /locus_tag="PLV77_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC14848.1"
                     /translation="MPVGYLITLFLLGTCVLLLLAPVRKRPLGLVSFWGGLLVNEQAH
                     WFAVLLAGSTVLAFVEGDISGVARAIAGLAVLAEAGLIVLFLRSFRSRGTLREALAAF
                     DSDSPAALAGRRSYLAALAAPPAMGRSRVRLKSDIPYGPVPGRANRLDLYLPRHGKVT
                     GPTMIYMHGGRFRSGNKRREAQAIKCQLARRGWSVISANYRLNPEGSFPDYLIDLKRV
                     IAWARGEGQAFGIDPERIFLAGGSAGAHIAAMAGLTAGKPELQPGFEQVNTAVSGVIG
                     IYGYYGGLESPGGRPPAVPSSPRRHVQPGAPPFLLIHGDHDSVVGRGGAAALVKELQG
                     AGNQASLAVLPGAEHTFDLLRSVRTENTIDSIVDFAAWAERSENRDLSQSRR"
     gene            2107..2865
                     /locus_tag="PLV77_03010"
     CDS             2107..2865
                     /locus_tag="PLV77_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14849.1"
                     /translation="MGAKGAKGNLPGGLRTGLVGLGLLALAITCLLTSPAPDRSNAET
                     RANAEPQGGILPCGSHVVLTAVYRNRDGRIHFEGVADPKFAGRQARIYDRDNHLVAAT
                     TVGEDGIFWANANSKDEGYTWLTRFIARVGGSESRWKRIGQAVGLRDRQPFELEPTAR
                     NHGKTLERTRVRVKVAGGHSNLLVVGLQVGCSRFDVIELASTYTDSNGVSDLNLPRPP
                     ADRPYWIFRVSTEDDWKISPPIVVKSVPESGPTG"
     gene            2938..>3144
                     /locus_tag="PLV77_03015"
     CDS             2938..>3144
                     /locus_tag="PLV77_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14850.1"
                     /translation="MTQEQLEAYHEYTRKHGVNRGLYLFARIVLQPFFLIYLRLSRQG
                     RGNAQIKGATIVAANHRSFLDPFVI"
BASE COUNT          551 a         1053 c         1015 g          525 t
ORIGIN      
        1 gcaaccagtt ctggagcccg aacagccagg acgtcctgct gcaggagcgc acccggacaa
       61 tcccggcgac cacgctctcg gagcatgttc agcagaaggg cctgactccg aaccggatcg
      121 cgatcgccca catcgagcgt ccggccggca agaagctgaa ggtcgtttcc tcggaggtcg
      181 gcaactgggc ggtcgctccc acccagtact tcccctcggt cgggtcgaac acctcggtca
      241 ccgtgaacgg caaggccggc ggcaccgccg acctggtcta caccgggaat ctccagaccg
      301 gaagctggtc ctccgagtac cacgactaca ccaaggacgg caagaccttc gtcaacggga
      361 cctacttcgt cagccgcacc atcgaagggg tctggaccct caaggccgat gtcacggtga
      421 ccggccagca cacgggccgg ctcaacatgg acatgacgat ccagggcggc gacccgctgc
      481 cgatcatgac cggcggcatc actgctgtct atgacggcaa gaaggctccg gccctgccca
      541 agctgggcgc ctgctacaag aaactcccgc agaagagcag gctcgccgcc caagtcaagt
      601 cgaagcgcgc cggcaaggcg accaaggtga ccgtaaccgt caccgccaac gtctacggcg
      661 acaggcgacc ggtccagaac gcgaaggtga agctcgccgg ccggatcgtc cgcaccgacc
      721 gctcgggcaa ggcgaagctc aaggtccgcg gccgcggcac gcggaaagtg ttggtcaccg
      781 ccggggacac cttcatcaag acctcgaagg tcgcgcacct gaagcagcgc tgaccaggca
      841 atcctcgtcc cttccgggtc ctgtcgggca acgggccggc cgattatggc cggcccgttt
      901 ttcgtgggag gaagccggca actagcttgt gggaatgccc gtcggctacc tgatcacgct
      961 ttttctgctc ggaacctgcg tgctccttct gctggctccg gtgcggaagc gaccactcgg
     1021 cctggtctcc ttctggggcg ggctcctggt caacgagcag gcgcactggt tcgccgtgct
     1081 gcttgccggt tcgaccgtgc tggccttcgt cgagggcgac atttccggtg tcgcccgggc
     1141 gatcgcgggg cttgccgtac tggccgaggc cggactgatc gtcctctttc tcaggtcgtt
     1201 tcggtcccgc gggaccttgc gggaagcgct ggccgctttc gattcagatt cacccgccgc
     1261 acttgcaggc cggcgttcat acctggcggc gcttgccgct ccgccggcga tgggccgaag
     1321 tcgggttcgg ctgaaatccg acattcccta cggaccggtc ccgggccggg ccaatcggct
     1381 cgatctgtac ctgccccggc acggcaaggt gaccggcccg accatgatct atatgcacgg
     1441 gggcaggttc cggtcgggta acaagcgaag ggaggcccag gcgatcaagt gccagctggc
     1501 ccgccgcggc tggtcggtga tcagcgccaa ctaccggttg aatcccgaag gatcgtttcc
     1561 cgactacctg atcgatctca aacgcgtgat cgcctgggcc cgcggggagg ggcaggcatt
     1621 cggcatagat ccggagcgga tctttctggc tggcggctcg gccggggctc acatcgcggc
     1681 gatggccggg ctgaccgcgg gcaagccgga gctgcaaccg ggattcgagc aggtgaacac
     1741 ggccgtctcc ggggtgatcg ggatctacgg ctactacggc ggccttgaat cacccggcgg
     1801 ccgccccccg gctgtcccct ccagcccccg gcgccacgtg cagccgggcg cacccccgtt
     1861 tctgctcatc cacggagacc acgacagcgt ggtcgggcgg ggcggagccg cggccctcgt
     1921 caaggaactc cagggcgccg gcaaccaggc gtcactggcc gtcctgcccg gggccgaaca
     1981 caccttcgac ctgctgcgct cggttcgcac cgaaaacacg attgactcga tcgtcgattt
     2041 tgccgcctgg gcagaacgtt cggaaaatcg tgatctttca caatcccggc gctaagatgg
     2101 gctgttgtgg gggcgaaggg agcaaagggg aatcttcccg gcgggctgcg gaccggcctg
     2161 gtcggtctgg ggctgcttgc gctcgcgatt acctgtctcc tgacttcgcc tgcgcccgat
     2221 agatcgaacg ccgagactcg ggccaacgct gagccgcaag ggggcatcct tccctgcggc
     2281 agccacgtgg tccttaccgc ggtctatcgc aacagggacg ggcggatcca tttcgaaggg
     2341 gtcgccgatc cgaaattcgc cggcaggcag gcccggatct atgaccggga caaccatctg
     2401 gtcgcagcga cgaccgtggg cgaggacgga atcttctggg cgaacgccaa ttcaaaggac
     2461 gagggttaca cctggctcac ccggttcatc gccagggtgg gtggcagcga gtcccgctgg
     2521 aaacggatcg gccaggcggt cggcctcagg gaccggcagc cctttgaact ggagccgacg
     2581 gcacgcaacc acggcaagac cctggagcgc accagggtca gggtcaaggt ggccggcggc
     2641 cacagcaatc tcctggtggt cggtctgcag gtcggctgtt cccgattcga cgtgatcgaa
     2701 ctcgcttcga cctacacgga ctcgaacggg gtgtccgacc tgaacctgcc ccgtcccccg
     2761 gcggatcgtc cctactggat cttccgagtc agcaccgaag acgactggaa gatctcgccc
     2821 ccgatcgtcg tcaaatcggt gccggaatcc ggcccgaccg gctgattcgc ccgggcccgc
     2881 ccgaaaacgg cgaggacccg ttgtaaccgg gtccgggggt ttgtgtagga ctcttttgtg
     2941 actcaggagc aactcgaggc ctaccacgag tacacgcgca agcacggggt gaaccgcggt
     3001 ctttacctct tcgcgcgcat cgttctgcag ccgtttttcc taatctacct gcggctttcg
     3061 cgtcagggca ggggtaacgc gcagatcaag ggcgcgacca tcgtcgccgc caatcaccgc
     3121 agcttcctcg acccgttcgt gatc
//
LOCUS       DAOQMF010000125         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16985_length_3144_cov_2.660408, whole genome shotgun sequence.
ACCESSION   DAOQMF010000125 DAOQMF010000000
VERSION     DAOQMF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16985_length_3144_cov_2.660408"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLV77_03020"
     CDS             complement(<1..376)
                     /locus_tag="PLV77_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC14851.1"
                     /translation="MNTNPSMNGAAGRILIADDEPSVRDSVGYALAQEGFEVTAAEDG
                     TDAEERLAGEFDFDLLILDIMMPGKSGLDICREVRSRSTVPIIMLTAKDAEVDKVVGL
                     EVGADDYVTKPFSLRELLGRVRA"
     gene            complement(373..2130)
                     /locus_tag="PLV77_03025"
     CDS             complement(373..2130)
                     /locus_tag="PLV77_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC14852.1"
                     /translation="MSGSPASSPRSSRGRRSRFPRRRHIGMRVWLAAGFTLVCFLTAG
                     ILYTFITDKSQDVLNERSTELAVGRTVRLADRLSEPDVDPKAELEAANGEGYTAWYFD
                     SDGDLVAPENPAATFPASKPYQEALDEALVGSRSSRDLKGGEIAIVGTTVGNEGEQVG
                     VVVGRYSRPVVIRSAIDTLRSESLRAALIAILIGTLVGVGIASVITGRLKRIAAKADD
                     LASGNFDVRLPVRGRDEIGDLARSLDSMRAALKESFGVLTADRDKLAAIFDGLGDAVM
                     VIDDDGSVRFHNSAANALLEQGGRAPEPMMSSVRRAAVEGFAAHPALRMGDRAYALQA
                     RSLPDEGAVLVVVRDRTDEMRKEFAERDFVSNAAHELRNPLAGISGAIEVLQSGAKDD
                     PPARDHFLNRLSADAERMSRLTQSLLTLARVEALGTGEVEVVDIGSAVKDVEAAVEVP
                     VGLELRTDVEPDLAAKGDPTLLRQVLIGLVTNACKNTPAPGSVTIRGRRVGDAEMLLE
                     VIDTGTGIPQAEIERVFERFYRRSETRRQEGFGLGLAIARRMADVMGGEMGANSVEGE
                     GSTFWVRLPLIDQSKTPIA"
     gene            2217..2933
                     /locus_tag="PLV77_03030"
     CDS             2217..2933
                     /locus_tag="PLV77_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC14853.1"
                     /translation="MRKLVFIPAWNEENSIEAVIADAREHVPDADVMVVDDGSTDRTA
                     ALARRAGALVASLPFNQGVGAAHQTAYIFALRHGYEICGQLDGDGQHPASEVTRLIAA
                     VEAGADLAVGSRFADDINLNIPEYKSTFARRIGQRLFSLLVSTSTRQTFTDTTSGMRA
                     LNERAMQLFADRYSSEFAEVESLQQAVRAGLVVREVPVHMLAREHGRSFIGPLTSAYY
                     IFKTLIVLLIGQLRPKQVEQ"
     gene            2930..>3144
                     /locus_tag="PLV77_03035"
     CDS             2930..>3144
                     /locus_tag="PLV77_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HNC14854.1"
                     /translation="MIWALAADTEPVVRLTPQARIIGAVLAIAFMPLILDLIRRDKLL
                     ERYSIVWFLLGIGMLLGLVVFQLGQPWL"
BASE COUNT          498 a         1071 c         1035 g          540 t
ORIGIN      
        1 gggcccggac ccggccgagc agctcccgca gcgagaaagg cttggtcaca tagtcatcgg
       61 ccccgacctc gaggccaacc accttgtcga cctcggcgtc cttggcggtg agcatgatga
      121 tcggcaccgt ggaacgggaa cggacctcgc ggcagatgtc gaggccggac ttgcccggca
      181 tcatgatgtc gaggatcagc aggtcgaagt cgaactcgcc ggccaggcgc tcttcggcat
      241 cggtgccatc ctcggcggcg gtcacctcga acccctcctg ggccagcgcg tagccgaccg
      301 agtcgcgaac cgaaggttcg tcgtcggcga tcaggatgcg cccggcggcg ccgttcatgg
      361 atggattggt gttcatgcga ttggtgtctt cgactggtct atcagaggaa ggcgaaccca
      421 aaaggtactg ccctcgccct ctacggaatt ggctcccatc tctccgccca tcacatcggc
      481 catcctgcgg gcgatcgcga gacccagtcc gaagccctcc tggcgccttg tttcggagcg
      541 ccggtagaag cgttcgaaga ccctttcgat ctccgcctgg gggatgccgg taccggtgtc
      601 aatcacctcg agcagcatct cggcgtcccc gacccggcga ccgcggatgg tcacagaacc
      661 gggggccgga gtgttcttgc aggcgttggt gaccagcccg atcagcacct gcctgagcag
      721 ggtcgggtca cccttggcag ccagatccgg ctcgacgtcg gttcgcagtt cgagaccgac
      781 cgggacctcg accgccgctt cgacgtcctt taccgcggag ccgatgtcaa ccacctcgac
      841 ctcgccggtg ccgagcgcct cgacccgcgc aagggtgagc agcgactggg tcagcctgga
      901 catccgctcg gcgtcggcgg agaggcggtt caggaagtga tcccgggccg gcgggtcatc
      961 cttggccccg gactgaagca cctcgatcgc ccccgagatg ccggccagcg gattgcggag
     1021 ctcatgggcc gcgttgctga cgaagtcgcg ttcggcgaac tccttgcgca tctcgtcggt
     1081 acggtcgcgg accacgacca gcaccgcccc ttcgtcggga agcgagcggg cctggagcgc
     1141 gtaggcgcgg tcacccatgc ggagcgccgg gtgcgcggcg aaaccctcga ccgcggcccg
     1201 tctgaccgag gacatcatcg gttcgggggc ccggccaccc tgttcgagca gggcgttcgc
     1261 ggccgagttg tggaaccgga ccgagccgtc gtcgtcgatc accatcaccg cgtcgccgag
     1321 cccgtcgaaa atggcggcca gcttgtcgcg atcggcagtc agcacgccga agctttcctt
     1381 cagggcggcc cgcatcgaat cgagcgaacg ggcgaggtcg ccgatctcgt cacggccgcg
     1441 gaccggcagg cggacgtcga agttgccgga agcgaggtca tcggccttgg ccgcgatgcg
     1501 cttgaggcgg ccggtgatca ccgaggcgat cccgactccg accagggtgc cgatcaggat
     1561 cgcgatcaat gccgcgcgca ggctttccga gcgcagggtg tcgatcgccg agcggatcac
     1621 aaccggccgc gagtaacggc cgaccacgac cccgacctgc tcgccttcgt tgccgaccgt
     1681 cgttccgacg atcgcgattt cgccgccctt caggtcccgg gaggagcgcg aaccgaccag
     1741 cgcctcgtcg agagcctcct ggtagggctt gctcgcgggg aaggtggcag ccgggttctc
     1801 gggggcaacc aggtcgccgt cggaatcgaa ataccaggcg gtgtaaccct cgccgttggc
     1861 ggcttcgagt tcggccttgg ggtcgacgtc gggctcgctg agccggtcgg ccagacggac
     1921 cgtgcggccg accgcgagtt cggtcgagcg ttcgttcagc acgtcctggc tcttgtcggt
     1981 gatgaaggtg tagagaatcc cggcggtcag gaaacagacc agcgtgaatc cggccgccag
     2041 ccagacccgc atgccgatgt ggcggcggcg cgggaagcgt gatcgcctgc ctcggctgga
     2101 tctagggctg ctggcggggc tgccgctcat gagcggaccc tagaggttgt agccaaactc
     2161 tgttcccggt gtggagactc tcggccacaa cctctatttc atgccagaat cgccgcgtga
     2221 ggaaactcgt cttcattccg gcctggaacg aggaaaactc gatcgaagcc gtgatagccg
     2281 acgcccggga gcatgttccg gacgccgacg tgatggtcgt cgacgacggt tcgaccgacc
     2341 gcaccgctgc cctggcccgt cgggccggtg ccctggtcgc ctcgcttccc ttcaatcagg
     2401 gggttggcgc cgcccatcag acggcctaca ttttcgccct gcggcacggc tacgaaatct
     2461 gcggccagct cgacggcgac ggccagcatc ccgcctcgga ggtcacccgg ctgatcgccg
     2521 cggtcgaggc cggagccgac ctcgcggtgg gttcccgctt cgccgatgac atcaacctga
     2581 acattcccga atacaagtcg accttcgccc ggcggatcgg ccagcggctg ttcagcctgc
     2641 tggtctcgac ctcgacccgg cagaccttca ccgacacgac cagcggcatg cgggccctga
     2701 acgaacgggc gatgcagctc tttgccgacc ggtactcctc cgagttcgcc gaggtcgagt
     2761 cgcttcagca ggcggtgcgc gccggcctgg tggtgcgcga agtcccggtc cacatgctgg
     2821 cccgcgagca cgggcgctcc tttatcggtc cgctcacctc cgcctattac atcttcaaga
     2881 ccctgatcgt gctgctgatc ggccagctcc gaccgaagca ggtggagcag tgatctgggc
     2941 cctcgcggcc gataccgagc cggtcgttcg gctgaccccc caggcgcgca tcatcggtgc
     3001 ggtcctggcg atcgccttca tgcccctgat cctcgacctg atccgtcgcg acaagcttct
     3061 cgagcgctac tcgatcgtct ggtttctgct cggaatcggc atgctgctcg gtctggtcgt
     3121 gttccagctc ggccagccct ggct
//
LOCUS       DAOQMF010000126         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16992_length_3143_cov_3.719883, whole genome shotgun sequence.
ACCESSION   DAOQMF010000126 DAOQMF010000000
VERSION     DAOQMF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16992_length_3143_cov_3.719883"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLV77_03040"
     CDS             <1..212
                     /locus_tag="PLV77_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14855.1"
                     /translation="EPLVAEARRRYRPNLVIAGGPDGSSTPPLMQDRTSLEGQPAAYV
                     CQSFTCQAPVNDPAELGKELDALDQ"
     gene            196..693
                     /locus_tag="PLV77_03045"
     CDS             196..693
                     /locus_tag="PLV77_03045"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNC14856.1"
                     /translation="MPSTSEIATHYFECVSRQDIPGMVACWKPGSRATFVGMDELNAP
                     EDYDQWFGNLFGCFPDFKLEAQSIVAEGEQASVHWRATGTFNGEGIFEGFRPNGASID
                     IEGVDLVTLEDGKIVHITAILNGLDMARQLGAVPAKGSIADKAMAAAFNTKTATIRKL
                     KSLRN"
     gene            complement(705..2057)
                     /locus_tag="PLV77_03050"
     CDS             complement(705..2057)
                     /locus_tag="PLV77_03050"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNC14857.1"
                     /translation="MAVQTNTPEAVFQAVRENDVRFVRLWFTDVLGNLKSFSINSSEL
                     ERAFEQGIGFDGASLTGFNPVEESDMVARPDASTFSLIPWRPGGTDAVARMFCDIQVP
                     GGLAYEGDPRWVLKRALERAADLGFDDFVVAPELEFFYFRSARPEGGVPEVLDEGGYF
                     DLTTLDAGSDVRRETVLALEQMGVHVASTHHEAGPSQHELDLKPAGALATADDVMTSK
                     IVVKEYAVQQGWHATFMPKPIGGANGSGMHLHQSLTRDGANAFYNGEKPGALSPTGKA
                     FIAGQLRHAREIAPLYAQWVNSYKRLVPGFEAPVYLSWSRRNRSSLIRVPQQNGEEPD
                     GAKVEIRCPDPACNPYLCLAGLLQAGLEGIEKGYELPEPMEENLYHLGPDDLAQRGIE
                     QLPATLGEAIELAADSELVLRTLGEHTFRRFIELKRQEWDSYRVQVTPFEVESFLPVL
                     "
     gene            2271..2837
                     /locus_tag="PLV77_03055"
     CDS             2271..2837
                     /locus_tag="PLV77_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNC14858.1"
                     /translation="MTVKTEVSRFHIHDELTAPDESAPILKSIQVGGRQVNKLVGVLA
                     GSPAVLRAYTRMRAELQQGELPQATRQRIALAVAEHRGDAYSVVQYAKSARNAGLGLD
                     EVSRARSFTSSDPKEQALLTYLKAALEADGDPPIYLHEEAREAGWDDEEILEALAHVA
                     MGEFQSVMAAAAALPKDQATPRVLPDAA"
BASE COUNT          593 a         1000 c         1020 g          530 t
ORIGIN      
        1 cggagccgct ggtggctgag gcccgccggc gctaccgtcc gaacctggtg atcgccggcg
       61 gaccggatgg atcatcgacc ccgccgctga tgcaggaccg gacttcactc gaaggccagc
      121 cagccgcata tgtttgtcag agcttcacct gtcaggcgcc agtcaacgac cccgcggagc
      181 ttggaaagga actcgatgcc ctcgaccagt gaaatcgcca cccactactt cgaatgtgtg
      241 agccgccagg acattcccgg catggtcgcc tgctggaagc cgggcagccg cgccaccttc
      301 gtcgggatgg acgaactcaa cgcccccgag gactacgacc agtggttcgg caacctgttc
      361 ggctgcttcc ctgacttcaa actggaggcg cagagcatcg tcgccgaggg cgagcaggcc
      421 tcggtccact ggcgggcgac cggcaccttc aacggagaag gcattttcga aggctttcga
      481 ccgaacgggg cgagcatcga catcgagggg gttgatctcg tcaccctgga ggacgggaag
      541 atcgtccaca tcacggccat tctcaacggc ctggacatgg cccgccagct cggcgcggta
      601 ccagcaaagg gttccatcgc cgacaaggca atggccgccg ccttcaacac gaagaccgcc
      661 acgatcagga agctgaagag cctgaggaac tgaccggagc cgggctaaag gaccggcagg
      721 aagctttcga cctcgaaggg ggttacctgg acgcggtagg aatcccattc ctggcgcttc
      781 agttcgataa agcggcggaa ggtgtgttcg cccagggtgc gcaggaccag ttccgagtcg
      841 gcggccagtt cgatcgcttc gccgagtgtg gccggcagct gttcgatgcc gcgctgggca
      901 aggtcgtcgg ggccgaggtg gtagaggttc tcctccatcg gctcgggcag ttcgtagccc
      961 ttttcgatcc cttcgagacc ggcctgaagc agcccggcga ggcaaaggta ggggttgcag
     1021 gcagggtcgg ggcagcggat ctcgaccttc gctccgtccg gctcctcgcc gttctgctgg
     1081 ggaacccgga tcagcgagga acggttgcgg cgcgaccagg aaaggtagac cggggcctcg
     1141 aagccgggca ccagccgctt gtacgagttg acccactgcg cgtagagcgg cgcgatctcg
     1201 cgggcgtgcc ggagctgacc ggcgatgaac gccttgccgg tcggtgaaag ggcgccgggc
     1261 ttctccccgt tgtagaaggc gttggcaccg tccctggtca gggactgatg aaggtgcatc
     1321 ccggaaccgt tggcgccacc gatcggcttc ggcatgaagg tcgcgtgcca gccctgctgg
     1381 accgcgtact ccttgaccac gatcttcgag gtcatcacgt catccgcagt ggccagcgcc
     1441 ccggccggct tgaggtcgag ttcgtgctgg gaaggcccgg cctcgtggtg ggtcgaagca
     1501 acgtggaccc ccatctgctc cagcgccagg accgtctcgc gccgcacgtc ggagccggcg
     1561 tcaagggtgg tcaggtcgaa gtagccgccc tcatcgagaa cctctggcac accgccttcc
     1621 gggcgggcgg accggaaata gaagaactcc agttcggggg cgaccacaaa gtcgtcgaaa
     1681 cctaggtccg cggcccgctc cagggcccgc ttcagaaccc agcgggggtc accttcgtag
     1741 gcgagaccgc ccggtacctg gatgtcgcag aacatgcggg cgaccgcgtc ggtcccgccg
     1801 ggtcgccagg gaatgaggct gaaggtcgaa gcgtcgggcc gggcgaccat gtccgattcc
     1861 tcgaccggat tgaaacctgt cagcgaggcc ccgtcgaagc cgatcccctg ctcgaaggcc
     1921 cgttcgagtt cgctcgaatt gatcgagaag ctcttcaggt tgcccagcac gtcggtgaac
     1981 cagaggcgga caaaacggac gtcgttttcc ctgactgctt gaaagacggc ttccggggta
     2041 tttgtctgta ctgccatcgg actccttgga ggggctaaat aagcggcttc gagccggaat
     2101 cgaggtgctt caggcggaaa cgatacgcga ggggcttgcg taacgccgcc ggagtgggta
     2161 taacttactt gcggtaagta actagctaaa agtaaagtaa tggctcgtta aacggacggt
     2221 aaaggccggg gacgagccgt aactcggaga acactgggaa gcgtgacatc gtgaccgtaa
     2281 agacagaagt cagccgattc catatccacg acgaactgac cgctcccgac gagagcgcgc
     2341 caattctcaa gagcatccag gtgggcggcc gccaggtgaa caagctggtc ggcgtgctgg
     2401 ccggctcccc cgccgtgctt cgcgcctaca cccgcatgcg tgcggaactc cagcagggcg
     2461 agcttcccca ggccacccgg cagcggatcg ctctcgcggt ggccgaacac aggggtgatg
     2521 cctacagcgt cgtccagtac gcgaagtctg cccgcaacgc cggcctcggc ctcgatgaag
     2581 tgtcgcgggc ccgcagcttc acttcctcgg acccgaagga gcaggcgctg ctcacctacc
     2641 tcaaggccgc gctcgaagca gacggcgacc cgccgatcta ccttcacgag gaagcccgcg
     2701 aggccggttg ggatgacgaa gagattctcg aagccctggc tcacgtggca atgggtgagt
     2761 tccaaagcgt gatggctgca gccgcagccc tcccgaaaga tcaggcaact ccgcgggttc
     2821 tgcccgacgc cgcctgatca cctccataaa aggaccctcc cccggggtcc gatcgaggcg
     2881 gcccctctct ctcccctggc cgacctctcc ccctaaaaaa aaggacggcc ccgatcgggg
     2941 ccgtcctttc tttcgtacct ctcgaaactg tgtcgaagcg ctagcggctc tcgacccgga
     3001 cgccgtatgt cagggcgccg ttgaaccggc aaccgtatga cgccaggctg ttgacgtcga
     3061 tctgcggctg cgaattcctc atcgtgactt tctgggtgca ggtcgtgcga tcgcggctgg
     3121 cgacccagct gttgctcgcg ttg
//
LOCUS       DAOQMF010000127         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_16995_length_3143_cov_3.145402, whole genome shotgun sequence.
ACCESSION   DAOQMF010000127 DAOQMF010000000
VERSION     DAOQMF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16995_length_3143_cov_3.145402"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..993)
                     /locus_tag="PLV77_03060"
     CDS             complement(154..993)
                     /locus_tag="PLV77_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNC14859.1"
                     /translation="MPEPLRRFGAACWEVLLSFDRSAGTRQAAQLSFFVLLSFPALIL
                     LAVWILSNIFDSPDVRQDLIREIINNLPIEEIQGKREIEDMLDELTKGAGGLGIFSIV
                     ILLYSVSSAIGGLRHAVETANEKGDSGPPFPQNKLLDIGITLVTLPAALIFVALALSR
                     DLARVVDDNVFLSWSADHLGGPLGIFAAALIFYTWIFWVLNPGPTSWTSAAAGSLVTS
                     ALIWLVYSGLRIWFGISGGGSAIYGALAGFLGLLLFLNLASIAVVMGAHVAAIWRVHR
                     EAG"
     gene            complement(1037..1465)
                     /locus_tag="PLV77_03065"
     CDS             complement(1037..1465)
                     /locus_tag="PLV77_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14860.1"
                     /translation="MPETSGSQPPGGSAAWASWVAFAGIMLMLIGVFSVIAGLAAVTD
                     DGYIANASLHEVFLVSFKALGVFWILLGILKVLAGWALIQGREWARIVTVILVFLSAI
                     SHLLDLTSQPYLSILMIALDLVILYAVTVRWEAAKIGMGD"
     gene            complement(1531..2490)
                     /locus_tag="PLV77_03070"
     CDS             complement(1531..2490)
                     /locus_tag="PLV77_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNC14861.1"
                     /translation="MEAALDPAGPPPAEGMVWIPGGTFTMGSERHYPEEKPCGPVSVD
                     GFWMDETAVTNRQFAQFVSDTGYVTTAEKSPDPAMYPGADPAMLVPGSITFAKPSGPV
                     DLSNPMQWWAWTPGAQWRHPWGPASNLDGKEDHPVTQVTWEDAEAYCRWAGKELPTEV
                     QWERAGRGGIEGAEFAWGDEIAPGGEEKMNRWIGEFPWKFRPRPGGARKPGTVAVRTF
                     EPNPFGLYEITGNAWEWTKSWYADSHPEPVSRCCAPENPLGPGVEASRDPASGIPRKV
                     LKGGSFLCAENYCSRYRPSSRIPESIESSTVHITFRCVVPDAG"
     gene            complement(2514..>3143)
                     /locus_tag="PLV77_03075"
     CDS             complement(2514..>3143)
                     /locus_tag="PLV77_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC14862.1"
                     /translation="DPAVRYDYEGLGGDLDLVIEANPAENGMVLGGHSMGCHTVARRA
                     LMDPRGVRALILIGPVFTGEADDDSRWDRRADALANGGPEAFAEIVAENSPNEEVREV
                     TYRLARDRARLHRHPEAVAMALREVPRSLPFEGVNRLAALDLPVLVVASHDEFDPGHP
                     RQVAELWAETIPGAELAGEEPGQSPLAWQGGLLSREIESFLGRHGLAAG"
BASE COUNT          571 a         1044 c         1026 g          502 t
ORIGIN      
        1 gtggacgcca tcggcgttca ccgtcaccac tcccggcacg atccagccgt cgatcgcttg
       61 cccgatccag tcagagaaga tcaggatgaa gaagagcgcc agtcgcggcg agatcagtgc
      121 gaagagaact acgaagcaac ccatgcctgc aatctacccg gcctcacggt ggacccgcca
      181 gatcgcggcc acgtgagccc ccatcacgac cgcgatcgag gccaggttga ggaagagcag
      241 gaggccgagg aacccggcca gcgccccgta gatcgcgctg ccgccgcccg agatcccgaa
      301 ccagatccgc aggcccgagt acacgagcca gatcagggcc gaagtgacca gtgaacccgc
      361 cgccgcgctc gtccagctcg tcgggcccgg gttcagcacc cagaagatcc aggtgtagaa
      421 gatcagggca gcggcgaata tgcccaacgg tccgccgagg tggtcggcgc tccacgaaag
      481 gaagacgttg tcatcgacga ccctggcgag gtcgcgggaa agggccagcg cgacgaagat
      541 cagcgccgcc ggaagggtga ccagggtgat gccgatgtca agcagcttgt tctgggggaa
      601 gggcgggccc gaatcgccct tctcgttggc ggtctcgacc gcgtgacgaa ggccgccaat
      661 cgccgagctg accgagtaga gcaggatcac tatggagaag attccgagcc cgccggcccc
      721 cttggtcagc tcatcgagca tgtcctcgat ttctcttttg ccctggatct cctcgatcgg
      781 gaggttgttg atgatctccc ggatcaggtc ctggcgtacg tccggcgagt cgaagatgtt
      841 gctgaggatc cagaccgcga gcaggatcag ggccgggaag ctcagcagca cgaagaacga
      901 cagctgggcc gcctgccggg tgcctgccga acggtcgaat gacagcagca cttcccagca
      961 ggcggccccg aaccgccgca gcggctcggg caccttcgaa gcccaggcct tcagttttga
     1021 agcccgggtg ggcgaatcag tcccccatgc cgatcttcgc cgcttcccag cggacggtca
     1081 cggcgtaaag gatgaccagg tcaagagcga tcatcaggat gctcaggtag ggctgcgagg
     1141 tcaggtcgag caggtggctg atcgccgaga ggaagaccag gatcacggtc acgatccggg
     1201 cccactcgcg gccctggatc aatgcccagc cggcaaggac cttgaggatt ccgagcagga
     1261 tccaaaacac cccgagcgcc ttgaagctga ccaggaaaac ctcatggagg gaagcgttgg
     1321 cgatgtatcc gtcatcggtc acggctgcca ggccggcgat gacgctgaac acgccgatca
     1381 gcatcagcat gatgccggcg aatgcgaccc aggaagccca ggcggcggat cctcccgggg
     1441 gctgtgaacc actggtttca ggcattgaac tcctccatcg gtttcatgtt tggcaggccg
     1501 ccgggggacg aacgggcggc tccatgaacc ctatccggcg tcggggacaa cgcagcggaa
     1561 ggtgatgtgc acggttgagc tctcgatgct ttccgggatt cgcgaggacg gccggtagcg
     1621 cgagcagtag ttctcggcgc agaggaacga cccgcctttc agcaccttgc ggggaatgcc
     1681 gctggccgga tcacggctcg cttcgactcc cgggccgagc gggttctcgg gggcacaaca
     1741 gcgactcacc ggttccggat gcgaatccgc gtaccagctc ttcgtccact cccaggcgtt
     1801 tccggtgatc tcgtaaagcc cgaagggatt cggttcgaag gtcctgaccg ccaccgtccc
     1861 gggcttgcgg gcaccacctg gccggggccg gaacttccac gggaactcgc cgatccagcg
     1921 gttcatcttc tcttcaccgc caggggcgat ctcatcaccc caggcaaact cggccccttc
     1981 gattccgcca cggccggccc gttcccactg aacttcggtt ggaagttcct tgccggccca
     2041 gcggcagtag gcctcggcgt cctcccaggt gacctgggtg accgggtggt cctccttgcc
     2101 gtccagattg ctggcgggac cccacgggtg ccgccactgg gcgccgggag tccaggccca
     2161 ccactgcatc gggttcgaga ggtcgaccgg gccggaaggc ttggcgaagg taatcgagcc
     2221 aggcaccagc atcgccggat cggcgcccgg atacatggcc gggtccggtg acttttccgc
     2281 cgtagtcacg tagccggtgt cggacacgaa ctgcgcgaac tggcggttgg tcaccgcggt
     2341 ctcgtccatc cagaaaccgt ccaccgaaac cggcccgcag ggcttctcct ccggatagtg
     2401 cctttcgctg cccatcgtga aggttccccc gggtatccag accatgcctt cggccggcgg
     2461 cggcccggct ggatcaagtg cggcttccat ggagaaaagg ctacccgccg cgctcagccg
     2521 gcggccagcc cgtggcggcc gagaaagctt tcgatttccc gcgaaagcag gcctccctgc
     2581 caggccagtg gtgactgacc tggttcctcg ccggcaagct cggccccggg gatcgtttcc
     2641 gcccacagtt ccgcaacctg gcggggatgg ccgggatcga actcgtcgtg gcttgcgacc
     2701 accagcaccg gcagatcaag ggcggcaagc cgattgacac cttcgaaggg aagcgagcgc
     2761 ggcacctccc gcagggccat ggcgaccgct tccgggtgac ggtggagcct ggcccggtcg
     2821 cgggcgagac ggtaggtcac ttcgcgaact tcctcgttcg ggctgttctc ggccacgatc
     2881 tccgcaaatg cttccggccc gccgttcgca agggcgtcgg cgcgacggtc ccatcgcgag
     2941 tcgtcgtcgg cctcgccggt gaacaccggt ccgatcagga tcagcgcccg caccccccgg
     3001 gggtccatga gggcccgtct ggcgacggtg tggcagccca tcgaatggcc tccgaggacc
     3061 atcccgttct ccgccgggtt cgcctcgatc accaggtcga ggtctccgcc caggccctcg
     3121 tagtcgtagc ggacggcggg atc
//
LOCUS       DAOQMF010000128         3140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17019_length_3140_cov_4.249919, whole genome shotgun sequence.
ACCESSION   DAOQMF010000128 DAOQMF010000000
VERSION     DAOQMF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17019_length_3140_cov_4.249919"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLV77_03080"
     CDS             <1..558
                     /locus_tag="PLV77_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNC14863.1"
                     /translation="IAGACFGTVALDSIIDGSAVAPTNVVIGLPSSGIHSNGYTLARS
                     ALDGIGMDDNRLGRPLGEVLLEPTEIYVKAVLDLLASPVRVHGLAHITSGGLDNLLRL
                     KAEVTYEITDPLAPQPVFELIAERAGVPDEEMYEVFNMGCGFCVVVPAEDESAALEVL
                     RGHYPEARRIGDVAAGDRRVVRRTR"
     gene            808..2316
                     /locus_tag="PLV77_03085"
     CDS             808..2316
                     /locus_tag="PLV77_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC14864.1"
                     /translation="MNHPPASSQMIGTVLSDRYRLEAKLGSGGMSTVYLARDEVLDRP
                     VAVKLMHREMTEQPDQLERFNQEARAVAKLSNPNVVAVIDAGEDQGRPYIVLEYVQGE
                     TLKQRIARVGALDATEALAYGLEVAQGLGVAHERNMVHRDVKPQNVLIDSTGRAKLTD
                     FGIARELNDEGVTATGRVIGTTDYVAPEQAMGKEVDPRSDIYSLGIVLYEMLTGDVPF
                     EADNQIGVAMKHVNEELPDVQVIRPDISAASARVVDRATAKNPDDRYQTIEEMIDDLQ
                     AALEVEAIRAGGTTGEATSVLDAVAPPRRQIPTSKTSPWPAVLMLLVALVIAAGTAYF
                     ISRGDTGGGGGGGAGGGGSNETQIALVGASDYDPQGDEEEHSEEIDYAIDDDRSASAW
                     TTETYETDSFGDKEGVGMYVDAGEPVEASEMEIRTPTPGWTLEVYGSNDPIPDDVSGW
                     TRLAQVPDVAEQQQIDLITAGTRYQYYLLWITDPAETDSGFGVAISDIRLYG"
     gene            complement(2353..>3140)
                     /locus_tag="PLV77_03090"
     CDS             complement(2353..>3140)
                     /locus_tag="PLV77_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC14865.1"
                     /translation="DVVRDPGVVHLTGVSRGSGGTRSGRWSSDNYGFKVIRHETRQLP
                     DSQITVVNGIPTTTVERALRDFAAEATPAEMTKALTQGEKERAICWGTLRELVADSAG
                     HKGNTMLSTEIEGWNPAFVDTGSDPEIDFLRMIRKREMPWPAVNVKLGHNVPDFLWWH
                     LRLAVELDPYGTHSGAGSHRRDHRKGIELETMGLRVVRFTGEDLYQHEDRTGDELWIV
                     IRQQSRLLRCPIFLPPGSPLAEVPQIQPLDGAKRAAPPVEAPD"
BASE COUNT          548 a         1032 c         1009 g          551 t
ORIGIN      
        1 atcgccggcg cctgcttcgg cacggtcgcc ctcgactcga tcatcgacgg atcggcggtc
       61 gccccgacca atgtggtgat cggcctgccc tcctccggga ttcattcgaa cggctacacc
      121 ctggcccgtt ccgccctgga cgggatcggc atggatgaca accggctcgg ccgcccgctc
      181 ggcgaggtcc tgctcgagcc gacagagatt tacgtcaagg cggtgctgga cctgctcgcc
      241 tccccggtcc gggtccacgg cctcgcccac atcacttccg gcggtctcga caacctgctc
      301 cggctgaagg ccgaggtcac ctatgagatc accgatccgc ttgctcccca gccggtgttc
      361 gagctgatcg ccgagcgggc cggcgtgccg gacgaggaga tgtacgaggt cttcaacatg
      421 ggctgcggtt tctgcgtggt ggttcccgcc gaagatgaat ctgccgccct cgaagtgctg
      481 cggggtcatt acccggaagc ccgccggatc ggtgacgtcg cggccggcga ccgtagggtc
      541 gtccggcgta cacgttgacc cttgaccggc aggtcatccg tcgcgctgac tgatcccccg
      601 gcgccggcac ttcgcaaatc cgctctgtca gcccttcgcc gggttttgat tggcagaaac
      661 caacacttcg gacctcctgc gagagggtca tactggaccg tgaagcgaat tgagcaattt
      721 ttcccgaatt tgtcagtccc gctgcggaaa ccggtgcctg cggttagcct ccggtgccta
      781 tgagcttgag cgggcaaaat cagtgaagtg aaccacccgc ctgccagctc acagatgatc
      841 gggacggtcc tttcggatcg gtaccggctt gaggccaagc tcggcagtgg cggcatgtcc
      901 acggtctacc tcgcccgcga cgaggtgctg gaccgcccgg tggcggtgaa gctgatgcac
      961 cgggagatga ccgagcagcc ggatcagctc gagcgtttca accaggaagc gcgggcggtg
     1021 gcgaagcttt ccaacccgaa cgtggtcgcc gtgatcgacg cgggcgagga ccagggccgg
     1081 ccctacatcg tgctcgagta cgtccagggg gaaaccctga aacagcggat cgcccgggtc
     1141 ggtgccctcg acgccaccga ggcgctggcc tacgggctgg aggttgccca gggcctcggc
     1201 gtcgcccacg agcgcaacat ggtccaccgc gacgtcaagc cgcagaatgt cctgatcgat
     1261 tcgaccggcc gggccaagct gaccgacttc ggcatcgccc gggaactgaa tgacgagggc
     1321 gtcaccgcga ccggtcgggt gatcggcacc accgattacg tggcccccga gcaggcgatg
     1381 ggcaaggaag tggaccctcg ctccgacatt tactcactcg gcatcgtgct ctacgagatg
     1441 ctgaccggcg acgtgccgtt cgaggccgat aaccagatcg gcgtcgcgat gaagcacgta
     1501 aacgaggagt tgccggacgt gcaggtgatc cggccggaca tctcggcggc ctcggcccgg
     1561 gtggttgacc gggcgaccgc caagaacccg gatgaccgct accagacgat cgaggagatg
     1621 atcgatgacc ttcaggccgc gcttgaagtg gaagcgatcc gggccggcgg caccaccggt
     1681 gaggcaacct cggtgctcga tgcggtcgcc cctccccgcc ggcagatccc gacctcaaag
     1741 acctcgccct ggccggccgt cctgatgctg ctggtcgccc tcgtgatcgc ggccggcacc
     1801 gcttacttca tctcccgcgg tgacaccggc ggcggcggtg gcggcggggc cggaggcggc
     1861 gggtcgaacg aaacccagat cgccctggtc ggggccagtg actacgaccc ccagggggac
     1921 gaggaagagc attccgagga gatcgactac gcgatcgacg atgaccgttc ggccagcgcc
     1981 tggaccaccg agacctatga aaccgacagc ttcggggaca aggaaggggt cgggatgtac
     2041 gtcgacgcgg gcgaaccggt cgaggcctcg gagatggaga tccggacccc gaccccgggc
     2101 tggaccctgg aggtctacgg gtcaaacgat ccgatcccgg acgacgtttc cggctggacc
     2161 cggctcgccc aggtccccga cgtcgccgaa cagcagcaga tcgacctgat caccgccggc
     2221 accaggtacc agtactacct cctctggatc accgaccccg ccgaaaccga ctcaggcttc
     2281 ggcgtcgcca tcagcgacat ccgcctctac ggctgagcca tcagaagtcg ggcggggtcc
     2341 gacccgccct cctcagtcgg gtgcctctac tggaggtgcc gcacgtttcg ccccatcaag
     2401 gggctggatt tgcggcacct ctgcgagggg agaccccggt ggaaggaaga tcgggcatcg
     2461 aaggagccgg gactgctgtc gaatgacaat ccagagttcg tcaccggtcc tgtcttcatg
     2521 ctggtagagg tcttcgcccg tgaagcggac tactcgcagg cccatggttt cgagttcaat
     2581 ccccttgcga tggtcgcgac ggtgagatcc cgctccgctg tgcgttccgt acgggtccag
     2641 ttcgaccgcc agccgtagat gccaccagag gaagtccggg acgttgtggc ccagcttcac
     2701 gttgaccgcg ggccagggca tttcccgctt gcggatcatt cgaaggaagt cgatctccgg
     2761 atcggagccc gtatcgacaa aggctggatt ccatccctca atctcggtcg acagcatcgt
     2821 gttccccttg tgtccggccg aatctgccac cagctcgcga agggtgcccc agcagattgc
     2881 ccgttccttc tctccctggg tcaatgcctt tgtcatttcg gccggagttg cttcggccgc
     2941 gaaatcgcgt agtgctcgct ccaccgtggt cgtcggtatt ccattcacga cggtgatctg
     3001 gctgtctgga agttgacggg tttcgtgccg tatgaccttg aagccgtagt tgtcgctgga
     3061 ccatctgccg gatcgggtgc cgccagaccc tctcgaaacg ccggtgaggt gaactacccc
     3121 gggatcgcga acaacatcgt
//
LOCUS       DAOQMF010000129         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17162_length_3127_cov_3.171549, whole genome shotgun sequence.
ACCESSION   DAOQMF010000129 DAOQMF010000000
VERSION     DAOQMF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17162_length_3127_cov_3.171549"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /gene="argF"
                     /locus_tag="PLV77_03095"
     CDS             <1..719
                     /gene="argF"
                     /locus_tag="PLV77_03095"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNC14866.1"
                     /translation="ISFEVGTVELGGHPVVLRGSEMQLSRGESVEDTARVLSRFVSAI
                     VIRSGSHENVVALAENASVPVINALTPEFHPCQALADLQTLKQRFGTLDGLKVAYVGD
                     GNNVARSLAIAGSLTGVEVVVAAPAGYQLETEVPAKLTDDPIEAVHRADAIYGDVWVS
                     MGDEASAGQRRADLAPYRIDGHLLSHARQETIVMHCLPAHPGEEITADVLYGDRSAVW
                     DQAENRLHAQKALLEYLLAP"
     gene            934..1005
                     /locus_tag="PLV77_03100"
     tRNA            934..1005
                     /locus_tag="PLV77_03100"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Glu,seq:ttc)
     gene            1084..1878
                     /locus_tag="PLV77_03105"
     CDS             1084..1878
                     /locus_tag="PLV77_03105"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNC14867.1"
                     /translation="MIPEISLGPITLQTFGLAFAAAFIVSGWIVHLRLEEIGKPPDWA
                     YEMVFAALIGGLIGARAYFIFDNYADVKDDLLGNIFSGSGLTWYGGAIGGAIGVILWA
                     WWRKFLNLWLLDTAAPALAAGYAVGRIGCQLSGDGDYGKPWDGPWAMSYPDGTVPTPD
                     GVTVHPTPIYETLTMGLGAYILWRLRDRFRLGILFAIYLIYAGTERLLVEFLRRNTDV
                     FLGLTQAQLESVAMILGGIIWILVVRRRYGSLARPADQMSAADVPA"
     gene            1949..2021
                     /locus_tag="PLV77_03110"
     tRNA            1949..2021
                     /locus_tag="PLV77_03110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1982..1984,aa:Val,seq:tac)
     gene            2075..2350
                     /locus_tag="PLV77_03115"
     CDS             2075..2350
                     /locus_tag="PLV77_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNC14868.1"
                     /translation="MSTGVQKLPGGVVHKLPADLRSGLIKHEVALAAWKDITPLARNE
                     FICWVEDAKKQETRERRIRRTREELEEGMRRPCCWPGCDHRERNGKA"
     gene            2404..>3127
                     /locus_tag="PLV77_03120"
     CDS             2404..>3127
                     /locus_tag="PLV77_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNC14869.1"
                     /translation="MEDGEQERAGRSRRVWVSEICWFLTIFFMLWAIVRTGGLERGFP
                     LVQLLAYTPYVLLLSLIALLIVVLCRRWLAAGFLLIACLALALAVLPREIGGPETVPG
                     GQPVRVMTINLGVGYAHADEIAELARVRDVDLVAVQELTPAEASGLDRAGFATDYPHR
                     ILQPGTEASGGGIYSRWPLVDRGSLTAEFHQPVADVRIPASIPIEFVSVHPMAPTTPA
                     RTSQWASEYKSLPEASSARLPLV"
BASE COUNT          476 a         1088 c         1017 g          546 t
ORIGIN      
        1 ggatctcgtt tgaggtgggg acggtggaac tcggcggcca cccggtcgtc ctgcgcggca
       61 gtgaaatgca gctttcccgc ggcgagtcgg tggaagacac cgcccgggtc ctttcccgtt
      121 tcgtctccgc aatcgtgatc cgctcgggca gccacgagaa cgtggtcgcg ctggccgaga
      181 acgcgagcgt tccggtgatc aacgccctca ctcccgagtt tcatccctgc caggcgctgg
      241 ccgacctgca gaccctgaag cagcgcttcg gcaccctgga tggcctcaag gtcgcctacg
      301 tgggggacgg gaacaacgtc gcccgctcac tcgcgatcgc cggctcgctg accggagtgg
      361 aggtggttgt cgccgctccc gccggctatc agctggagac cgaggttccc gcgaagctga
      421 ccgatgatcc gatcgaggcg gttcaccgcg ccgatgcgat ctacggtgac gtctgggtca
      481 gcatgggcga cgaagcgagc gccgggcagc ggcgggccga cctcgctccg taccggatcg
      541 acggccacct gctttcgcac gccaggcagg agacgatcgt catgcactgc ctgccggccc
      601 atcccggcga ggagatcacc gccgacgtgc tttacgggga tcgcagcgcg gtctgggacc
      661 aggcggagaa ccgcctccac gcccagaagg ccctgctcga gtacctgctc gccccctgac
      721 acccgctcgc cccctgacac ccgctcaccc ctgacacccg ctcacccctg acacccgctc
      781 gcccctgaca cctgctcgcc ccctggcctc gggatctgcc cgaatcgaga gggctaaatg
      841 gagcgctata tggccacttt tgccttctcg cgcggccggg gaaccggtcg gcaagccgcc
      901 actcgcgttc aactgagaga atgtccgccg cgcggccccg tggtgtaacg gttagcacgc
      961 caggttttca acctggaagc gcgggttcga ctcccgccgg ggctactgct cgccctttgc
     1021 cagggccggc gaagcggtcc ggccggaatc gcaggcctga cccgcaccgg tagggtccgg
     1081 ggcgtgattc cggagatctc gctcggcccg ataactctgc agaccttcgg tctcgctttc
     1141 gcggccgcct tcatcgtttc cggatggatc gtccacctgc gactggagga gatcggcaaa
     1201 ccgccggact gggcatacga gatggtcttc gccgcgctga tcggcggttt gatcggggcc
     1261 cgggcctact tcatcttcga caactacgcc gacgtcaagg acgacctgct cggcaacatc
     1321 ttcagtggtt ccggcctgac ctggtacggc ggcgcgatcg gtggcgcgat cggggtgatc
     1381 ctctgggcct ggtggcggaa gttcctcaac ctgtggctgc tggacaccgc cgcgccggct
     1441 ctcgcagcgg gttatgcggt cggccggatc ggctgccagc tctccggtga cggggattac
     1501 gggaagccct gggacggacc gtgggcgatg agctacccgg acggcaccgt cccgaccccc
     1561 gacggtgtca ccgtccaccc gaccccgatc tacgagaccc tgaccatggg gctgggcgcg
     1621 tacatcctct ggcgcctcag ggaccggttc cggctgggga tccttttcgc gatctacctg
     1681 atctacgccg gaaccgaacg tctcctggtc gagttcctgc ggcggaacac cgacgtcttt
     1741 ctcgggctca cccaggccca gctcgagagc gtggcgatga tcctcggcgg catcatctgg
     1801 atcctggtcg tcaggcgccg ctacggcagc ctcgcccgcc cggctgacca gatgagcgct
     1861 gccgacgtcc ccgcctgacc gtaccgcgaa cgaccgggca taatcccctt tccggttcgc
     1921 gcaccttcct tcaggtccgc ggaccccggg gcggttagct cagttggtag agcgcctgct
     1981 ttacacgcag gatgccgggg gttcaagtcc ctcaccgccc atctcctgcc cgggctgttc
     2041 gaggtctcat tgacccatca cctacgatgg aggcatgagc accggcgttc agaagttgcc
     2101 cggcggggtg gtgcacaagc tgcccgccga cctgcgtagc gggttgatca aacatgaagt
     2161 ggcgcttgcg gcgtggaagg acatcacgcc gctggcccgc aacgagttca tctgctgggt
     2221 cgaggatgcg aagaagcagg aaacccggga acggcggatc cgccggacca gggaagagct
     2281 cgaggaaggc atgcgccggc cctgctgctg gcccggctgc gaccatcgcg agcgcaacgg
     2341 caaggcttag ccccgaatcc cggatcgggc tgaaccatcc gcgatgtcgg gataattccc
     2401 ggcttggaag acggcgagca agagcgagcg ggccggagcc ggcgggtctg ggtctcggag
     2461 atctgctggt ttttgaccat cttcttcatg ctctgggcca tcgtccgtac cggtggtctg
     2521 gaacgcggct tcccgctggt tcagcttctc gcctacacgc cgtacgttct gctgctctcg
     2581 ctgatcgccc tcctgatcgt cgtgctctgc cgacgatggc tggcggccgg gttcctgctg
     2641 atcgcctgcc tggctctcgc gctggccgtc ctgccgcggg agatcggcgg gccggagacg
     2701 gtcccgggcg gtcaaccggt gcgggtgatg acgatcaacc tcggggtcgg gtacgcccac
     2761 gccgatgaaa tcgccgagct cgcccgggtc cgggacgtcg acctcgtcgc ggttcaggaa
     2821 ctgaccccgg cagaagcctc cgggctcgac cgggccggct tcgcgaccga ctatccccac
     2881 cggatccttc agccggggac cgaagcgtcc ggcggaggca tctactcccg ctggccgctg
     2941 gtcgaccggg gaagtctgac cgccgagttc caccagccgg tggccgacgt gcggattccc
     3001 gcctctatcc cgatcgaatt cgtctcggtt cacccgatgg ccccgaccac gccggcccgg
     3061 acctcgcagt gggcctccga gtacaagtca ttgccggaag cctccagcgc caggctgccc
     3121 ctggttc
//
LOCUS       DAOQMF010000130         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17201_length_3123_cov_3.177314, whole genome shotgun sequence.
ACCESSION   DAOQMF010000130 DAOQMF010000000
VERSION     DAOQMF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17201_length_3123_cov_3.177314"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PLV77_03125"
     CDS             complement(<1..1410)
                     /locus_tag="PLV77_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14870.1"
                     /translation="MGGLAALVAFFAVAVLAGTPARAATNEELSSCSEIKQTIEQPDG
                     SIVGMGDCRYGVSPAPRIVLVGLNPDGSDRTAFADGGAALISVEENEFGLKIIGRPDG
                     SFLVLSQKRIWSFDGDGDPVESFGNHGVLNFSGADQMNLASAFDFALDSGGNLLVAGR
                     RPSTATPVVGKYDQTGQLVSGYGNSGVSAELDLNFDAAIAVDNSNRVVMVGEDGATRL
                     LADGSLDLSFGPENDGYADLPAGLPYSIGEVFGLTVHPDDSITAYLDRTPGMYYYPAY
                     AVSLDSDGSPAAEDALVALGDHVSAVAPIEGGVAFVGFPGRGFQHWQFEVGTTNWPDI
                     RSVTFSPGDSLAHGVTALSDGSLLVSGNAQGPDCSDSCVFRNHLALAKIDPGTGDLVE
                     SFGSGGERLIPPNGCSRGQAGKAGDWNICRVRPPGINGSVRFAGAGSKHPSLVLKASL
                     GDPPEGLVGTRQRLTVDLPR"
     gene            complement(1477..2310)
                     /locus_tag="PLV77_03130"
     CDS             complement(1477..2310)
                     /locus_tag="PLV77_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNC14871.1"
                     /translation="MRGGSARKLRVALVAVAAASAAFLFAAPATANNTWVSISDFQWS
                     DKNPHIDLGESVTWLWAGPDTPHSVTGQAPRDSAFDPITTTDASQWDSDAGRNEPNHW
                     PGDEYTITFDHPGTYLFACKLHSVVRGEVTVSNLPGNPDSSRGPRPKINFDFDSPVVD
                     DYFFTADGESPAPPVVGPRGRGIGFRFSVNEATTASADYYQLVTRGKGSRKHLVRVYR
                     GYGEWPAHVGINRVGFARPTANFKPAPGRYLAWFRAEDRVQNSTGDIPLRFTISPGKG
                     G"
     gene            complement(2313..>3123)
                     /locus_tag="PLV77_03135"
     CDS             complement(2313..>3123)
                     /locus_tag="PLV77_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNC14872.1"
                     /translation="FAAAVVASAFTAPASANNTRVTIADFHWSNKNPEVDLGESVLWT
                     WTGPDTQHSVTGQPAGNSGAESGLPGIGTVATDDASQWDSDPDTNYPIHYPGSEYKVT
                     FDHPGKYLFVCKVHASVRGVVTVSDTPGNPDSDFGPAPVINFDTEGPLIDTYFFTSNG
                     TDPTMPFIGHKGKGIGFSFGTNEGGSASADYYRLVKRGKGKKARYVKFFQGYNEWPTH
                     IGMNNVRFAARSSTFKPVPGKYVAYFRAEDGKSNSSDDITLNFTVFPKKKR"
BASE COUNT          557 a          978 c         1028 g          560 t
ORIGIN      
        1 ccttggcaga tcgacggtga gcctttgccg ggtaccgacc aatccttccg gcgggtcgcc
       61 gaggcttgcc ttgagtacca gcgacggatg tttacttccg gctccggcga acctgaccga
      121 accgttgatc ccgggcggtc gcacccgaca gatgttccag tcacccgcct ttccggcctg
      181 cccccggctg catccgttcg gcggaatcag gcgttcaccg ccggaaccga agctctcgac
      241 gagatcgccg gtccccgggt cgatcttggc cagggccagg tgattccgga agacacagga
      301 atcggagcag tccgggcctt gcgcgttgcc agacaccagc agcgatccgt cgctcagcgc
      361 ggtgaccccg tgcgcaagcg aatcaccggg cgagaaggtc accgaacgga tgtccggcca
      421 gttggttgtt ccgacctcga actgccagtg ctggaacccg cgcccgggga aacccacgaa
      481 cgcgacaccg ccttcaatcg gggcgaccgc cgacacatgg tcgccgagcg ccaccaatgc
      541 atcttcagcg gctggagatc cgtcgctgtc cagcgaaacg gcgtaggcag ggtagtaata
      601 catgcccggt gtccggtcga ggtaggcggt gatcgagtca tccggatgga cggtcaggcc
      661 gaagacctcg ccgatgctgt aggggagccc ggcggggagg tccgcgtatc catcattttc
      721 gggaccgaac gaaaggtcga ggctcccgtc ggcaagcagg cgggtcgccc cgtcctcccc
      781 gaccatgacc acgcgattcg agttgtcaac cgcgatcgcc gcgtcaaaat tcagatcgag
      841 ttcagcgctc acgccagagt tcccgtagcc agagacaagt tgaccggttt ggtcgtactt
      901 gccgaccacc ggcgtggctg ttgaaggacg cctgcccgca accagcaggt tcccgccgga
      961 gtccaacgcg aaatcgaagg cggaggcgag gttcatctgg tcggcgccgc tgaaattcag
     1021 gactccgtgg ttaccgaagc tctcgaccgg gtcaccgtcc ccgtcaaatg accagatccg
     1081 tttctggctg agaaccagaa aacttccgtc cggacggcca atgatcttca ggccgaattc
     1141 gttctcctcg acggagatca atgcagcccc gccgtcggca aaagccgtgc ggtccgaccc
     1201 gtccggattg agcccgacca gaacgatccg gggcgcaggt gaaaccccgt agcggcagtc
     1261 ccccatgccc acgatcgaac cgtccggctg ctcgatcgtc tgcttgattt ccgagcagga
     1321 ggaaagttct tcgttcgtcg cagcccgcgc cggagttccg gccaggaccg ccacggcgaa
     1381 gaacgcgacc agcgccgcca atccccccaa ggatgaagtt ttcagtcccg gcgttcctgc
     1441 cattttcccg actctccttg acttgagcgt atcgcctcag ccgcccttgc cgggcgaaat
     1501 cgtgaaccgg agcgggatgt ctccggttga gttctggacg cggtcctcgg cgcggaacca
     1561 ggcgaggtag cggcccgggg caggtttgaa attcgcggtg ggtctggcga aaccgacccg
     1621 gttgattccg acgtgtgcgg gccactcgcc gtagccccgg tacaccctca cgaggtgctt
     1681 cctgctgccc ttgccgcggg tgaccagctg gtaatagtcg gccgaggcgg tggtcgcctc
     1741 gttcaccgag aaacggaaac cgatgccgcg gcccctcgga ccgaccaccg gcggggccgg
     1801 gctctcgccg tcggcggtga agaagtagtc atcgaccacg ggtgagtcaa agtcgaagtt
     1861 gatcttcggc ctcggtcccc ggctggaatc cgggttgccc ggcaggttcg agacagtgac
     1921 ctcgcccctg accaccgaat ggagtttgca ggcgaacagg taggtgccgg gatggtcgaa
     1981 ggtgatcgtg tactcgtccc cgggccagtg gttcggctcg ttcctgccgg cgtccgaatc
     2041 ccactggctg gcgtcggtgg tggtgatcgg gtcgaaggcc gaatccctgg gcgcctggcc
     2101 ggtgaccgaa tgcggggtgt cgggcccggc ccagagccag gtcaccgatt cgccgaggtc
     2161 gatgtgcggg ttcttgtccg accactggaa gtcggagatc gaaacccagg tgttgttcgc
     2221 cgtggccggg gcggcaaaca ggaaggcagc cgaagccgcc gcgaccgcga ccagcgcgac
     2281 gcgaagtttc ctggcggaac ctccgcgcac tcctagcgct tcttcttcgg gaagacggtg
     2341 aagttcaggg tgatgtcgtc agaggagttc gacttgccgt cttcggcccg aaaataggcg
     2401 acgtacttgc ccggcaccgg tttgaaggtg ctcgagcggg cggcgaacct gacgttgttc
     2461 atgccgatgt gggtcggcca ttcgttgtag ccctggaaga acttgacgta cctggccttc
     2521 ttgcccttgc cgcgcttgac caaccggtag tagtcggctg aagcggagcc accctcgttg
     2581 gtgccgaagg agaatccgat gcccttgccc ttgtgaccga tgaacggcat ggtcgggtcg
     2641 gtgccattcg aggtgaagaa ataggtgtcg atcagcggac cctcggtgtc gaaattgatc
     2701 accggggcgg gcccgaagtc cgaatccgga ttgcccggcg tgtccgagac cgtgaccaca
     2761 ccgcggaccg aggcgtgaac cttgcagacg aagagatact tgcccgggtg atcgaaggtg
     2821 accttgtact cactccccgg atagtggatc gggtagttcg tgtctggatc cgaatcccac
     2881 tgggaggcat cgtcggtcgc cacggtgccg atgccgggca gcccgctttc ggcgccggaa
     2941 tttccggcgg gctgaccggt gaccgaatgc tgggtgtcgg ggccggtcca ggtccagagc
     3001 acggactcgc cgagatcgac ctcggggttc ttgttcgacc agtggaagtc ggcgatcgtg
     3061 acccgggtgt tgttcgcgga cgccggggcg gtaaaagcgg aagcgacgac cgcggccgca
     3121 aat
//
LOCUS       DAOQMF010000131         3119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17238_length_3119_cov_4.531332, whole genome shotgun sequence.
ACCESSION   DAOQMF010000131 DAOQMF010000000
VERSION     DAOQMF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17238_length_3119_cov_4.531332"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLV77_03140"
     CDS             complement(<1..466)
                     /locus_tag="PLV77_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14873.1"
                     /translation="MVAVFAAGLVMLAAATPARAAWGTPILGNAPAGVATDSSGNLYV
                     AEAYGNRFVKYSATGTQLMAVGTTGSGQAQFNSPNGIAVESGGAIFVADTGNNRIQRF
                     YPNGSYQTEWGTFGTALGQFKQPAAVAVDGGGNVYIADTDNNRVLVYNGPWSD"
     gene            complement(709..1062)
                     /gene="rplQ"
                     /locus_tag="PLV77_03145"
     CDS             complement(709..1062)
                     /gene="rplQ"
                     /locus_tag="PLV77_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNC14874.1"
                     /translation="MRHAKKRNKLGRDSAHRKALLSNLSKEIIEHERIKTSQAKAKAA
                     KPEIEKLITLGKRGDLHARRQALSVLRQDKFAVHKLFEEIAPRYAERNGGYTRIVKIG
                     PRKSDSTEMVFLELV"
     gene            complement(1092..>1660)
                     /locus_tag="PLV77_03150"
     CDS             complement(1092..>1660)
                     /locus_tag="PLV77_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNC14875.1"
                     /translation="RLEMYVTIGRGRGYRPAEENKDADQPIGVIPIDSIFSPIRRASY
                     TVEAARVGQRTDFDKLTLELETDGSIEPNTALREASEILIKSLAIFSDPDRVEELTAR
                     EPVVAEGIVADNGAPVATGGDNDILIDELELSVRSYNCLKRAGIETIGQLLAKSESEL
                     AAIPNFGSKSIEEVIENLEGRGHHLRQD"
     assembly_gap    1661..1690
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1691..2064)
                     /locus_tag="PLV77_03155"
     CDS             complement(<1691..2064)
                     /locus_tag="PLV77_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNC14876.1"
                     /translation="MSTDFQVPTISSEQVEENKGVFTIEPLDKGFGYTFGSSLRRVLL
                     SSLGGAAITSVRIEGVSHEFSNIAGVKEDVTDIVLNLKDLVVRMHTDADAVEAPIVAT
                     GPGNVTAADIDLPAGVEILNPDA"
     gene            complement(2114..2506)
                     /gene="rpsK"
                     /locus_tag="PLV77_03160"
     CDS             complement(2114..2506)
                     /gene="rpsK"
                     /locus_tag="PLV77_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNC14877.1"
                     /translation="MAAKKPAKSRRRVRKNIAVGQAHIKTSFNNTIVTLTDPEGNVIA
                     WESAGSAGFKGSRKSTPFAAQVTADAAAKKGMEHGLQKVEVFAKGAGSGKDTAVRSLQ
                     AAGLEVTSVKDVTPQAHNGCRPRKRRRV"
     gene            complement(2524..2901)
                     /gene="rpsM"
                     /locus_tag="PLV77_03165"
     CDS             complement(2524..2901)
                     /gene="rpsM"
                     /locus_tag="PLV77_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNC14878.1"
                     /translation="MARIAGVNIPLNKRAEIGLTYVYGIGQSTAQEILDQVGIDRNTQ
                     VKDLTEDEVIKLRDAVEQHEVEGDLRRERSQNVKRLMEIGSYRGLRHRRGLPVRGQRT
                     KTNARGRKGPRRMSVAGKKKPAK"
     gene            complement(2905..3018)
                     /gene="rpmJ"
                     /locus_tag="PLV77_03170"
     CDS             complement(2905..3018)
                     /gene="rpmJ"
                     /locus_tag="PLV77_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNC14879.1"
                     /translation="MKVRASVKPMCEKCKIIRRRGRVLVICENPRHKQRQG"
BASE COUNT          502 a          926 c         1035 g          626 t
ORIGIN      
        1 cgtcgctcca cggcccgttg tagacgagga cacggttgtt gtcggtgtcg gcgatgtaga
       61 cgttgccgcc accgtcgacc gccaccgcgg caggctgctt gaactggccg agcgcagtgc
      121 cgaaggtgcc ccattcggtc tggtagctgc cgttcgggta gaagcgctgg atccggttgt
      181 tgccggtgtc ggcgacgaag atcgccccgc cggactcgac cgcgatgccg ttcggcgagt
      241 tgaactgggc ctggccggaa ccggtggttc cgaccgccat cagctgggtg ccggtcgcgc
      301 tgtacttgac gaagcggttg ccgtaggcct cggccacgta aaggttgccg gaactgtcgg
      361 tcgccacacc cgccggagcg ttgccgagaa tcggcgttcc ccaggccgcc cgggccggag
      421 tggcagcagc gagcatgacc agtcctgccg cgaaaacggc gaccatcgcg gcgaaaattg
      481 ccgcccggtt tgtgtgatgc cccctgttgg aaaacaactg cctgatccag ttcctttgaa
      541 ttcctaagta gcccagtata ggcgcagtat cagaaacacc aaataaccgg aaaagaagcg
      601 ctttcaaacc ggttccacgg gcccgatgga ggttggagca agcgaaaggg gagccgatgg
      661 ctcccctctt gcggcgactt gcctgttcag aacccgtctg accggagatc agacaagttc
      721 gaggaagacc atttcggtcg aatcggactt gcggggaccg atcttgacga tccgggtgta
      781 gccgccgttc cgctcggcgt agcggggggc gatctcctcg aacagcttgt ggacggcgaa
      841 cttgtcctgg cgcaggaccg aaagggcctg gcgccgggcg tgaaggtcac cgcgcttgcc
      901 gagggtgatc agcttctcga tctccggctt ggcagccttg gccttggcct ggctggtctt
      961 gatccgctcg tgctcgatga tctccttgga gagattgctg agcagcgcct tccggtgggc
     1021 ggaatcccgt cccagcttgt tccttttctt tgcgtggcgc atcgtctctc cttaagggtt
     1081 actcggtaat gctagtcctg gcgcaggtga tgcccgcggc cttcgaggtt ctcgatgacc
     1141 tcttcaatcg acttcgaacc gaagttcggg atcgcggcaa gctcggattc ggacttggcc
     1201 agcagctggc cgatcgtctc gatgccggcc cgcttgaggc agttgtaaga acggacgctg
     1261 agctcgagtt cgtcgatcag gatgtcgttg tcgccgccgg tggcaaccgg ggcgccgttg
     1321 tcggcgacga ttccttcggc aacgaccggc tcgcgggcag tcagctcttc gacccggtcc
     1381 gggtcactga agatcgcgag gctcttgatc aggatctcgg aagcttcgcg gagcgccgtg
     1441 ttcggttcga tcgaaccgtc ggtctccagc tcgagggtga gcttgtcgaa gtcggtgcgc
     1501 tgaccgacac gggcagcctc gacggtgtag gaggcgcggc ggatcggcga gaagatcgag
     1561 tcgatcggga tcacgccgat cggctgatcg gcatccttgt tctcctcggc cgggcggtaa
     1621 ccgcggccgc ggccgatggt cacgtacatc tcgaggcggg nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn gcgtcagggt tgaggatctc gacgccggcc ggcaggtcga tgtcggcggc
     1741 ggtgacgttg cccggacccg ttgccacaat tggcgcctcg acggcgtcgg cgtcggtgtg
     1801 catccggacc acgaggtcct tgaggttcag gacgatgtcg gtgacgtctt ccttcacgcc
     1861 ggcgatgttc gaaaactcat gcgaaacgcc ctcgatccgg acgctggtga tcgcggctcc
     1921 gcccagcgaa gagagcagca cgcgacgcag gctcgaaccg aaggtgtagc cgaagccctt
     1981 gtcgaggggc tcgatggtga aaacaccctt gttctcttca acctgctcgg acgagatggt
     2041 cggtacctgg aaatcagtgc tcattggatc cttttgttgc gggggagggg aaggcttgcg
     2101 gggcgcccgg aaatcagacg cgacggcgct tgcgggggcg gcagccgttg tgggcctggg
     2161 gcgtcacgtc cttgacgctg gtcacctcaa gaccggcggc ctgcagcgag cggaccgcgg
     2221 tgtccttgcc ggaaccggcg cccttggcga agacttcgac cttctgaagg ccgtgctcca
     2281 tgcccttctt ggcggcggcg tcggcggtca cctgggcggc gaagggggtc gacttgcgcg
     2341 aacccttgaa gccggcgcta ccggccgatt cccaggcgat gacgttgccc tcgggatcgg
     2401 tcagggtgac gatcgtgttg ttgaaggagg tcttgatgtg cgcctggccg accgcaatgt
     2461 tcttgcggac gcgacgacgg gacttggccg gtttcttggc tgccatgaaa gttctccagc
     2521 tccttactta gcgggcttct tcttgcccgc aacgctcatg cggcgagggc ccttgcggcc
     2581 ccgggcattg gtcttggtgc gctggccccg aaccggcagg ccgcggcggt ggcgcaggcc
     2641 ccggtaggag ccgatctcca tcaggcgctt gacgttctgc gagcgctcgc ggcgaaggtc
     2701 accttcgacc tcgtgctgct cgaccgcgtc acggagcttg atgacctcgt cctcggtcag
     2761 gtccttgacc tgggtgttgc ggtcgatgcc gacctggtcg agaatctcct gggcggtcga
     2821 ctgaccgatc ccgtaaacgt aggtgaggcc gatttcggcc cgcttgttga ggggaatgtt
     2881 taccccggcg atgcgcgcca tctactaacc ctgcctctgc ttgtgtcgcg ggttctcaca
     2941 gatcaccagg acgcgtccgc gccggcgaat aatcttgcac ttctcacaca tcggttttac
     3001 cgaggctcgg accttcatcg tcctaacgtt ccttttggag agatgtcccg agaatcccag
     3061 taaacggact ccgggcgtga aaaaagggcc aattttggcc gcttgggaac aatagcagg
//
LOCUS       DAOQMF010000132         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17328_length_3110_cov_3.554828, whole genome shotgun sequence.
ACCESSION   DAOQMF010000132 DAOQMF010000000
VERSION     DAOQMF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17328_length_3110_cov_3.554828"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PLV77_03175"
     CDS             <1..1213
                     /locus_tag="PLV77_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14880.1"
                     /translation="SGYPRALTVFKGQLYFAAGTPASGQELFRYDGTTVEQFTDIKPG
                     AGDAWAGFGKDTILDGYAYFLADDGVHGMELWRTDGENTELWLDATPGAEGSYFFSLK
                     AAGGRLYFATRTDYGLNTRAWRSDGVTNDVLGNFEIWQTTQVGQRVYLLSGHSLMVVT
                     GPDRQPEYKSLPFSAEVDNLTAIGNSLYFTATDRVHGSEPWRIKDGKLELLGSEAYDD
                     QGSIGWYPGQTGPFVEFDGEAFITMSDSRYGSELWKVQDGKAVLFKDILPGRDSGLPG
                     LYTHFGDEVYFIADDGVHGHELWHLFEDDEVGIVVPAQSPKIRNQATSISLGCPASEQ
                     AGPCRGSLKISRKGRTLGRGSFRIGAGHTGKPVVKLGPRVAAGPVSLEVTVRDDAGNS
                     KTVSRKLRLSR"
     gene            1210..2562
                     /locus_tag="PLV77_03180"
     CDS             1210..2562
                     /locus_tag="PLV77_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14881.1"
                     /translation="MRPLARIAAVAVALISAGLIAGTAQAAVRAELAADVDPGPSSSR
                     PSKLTAVGDSVYFYADGGPDRQGLWRTNGRLTQLVRPVGDPDLRIYWTEKVAFQGRLY
                     LMGSETGNNYRLWRTVPGGGRIEPVPGWPKELKAGSKPTVANGLLFFRGGDGLHKNEL
                     WKYDGERASLVKDINPGPAGSFPGYITGLGNQVIFNATDGVHGGELWRSDGTASGTRM
                     IGEINPGAKGTNMAWLTRVGDSVYLTADTPGHWLEPWRTDGEQLGLVAAEHEGPEWDN
                     ITSFAGLGGKVYFTANNELWESDDAWPEAEVHPIADDPDFPTTAKNAPVAAGNRIFFA
                     SFAGDWTGTMQSWPSFYDGEKIVTLTDPEEEFDYPWGYPQFFKEFGGRVFFVGGDTLH
                     GTELRVVSGRHYRLFADILEGDSSSRPRGFARSKHFMYFVADDGIHGREIWRLRRTAG
                     "
     gene            2715..>3110
                     /locus_tag="PLV77_03185"
     CDS             2715..>3110
                     /locus_tag="PLV77_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14882.1"
                     /translation="MYPEIHRLRFVLAAASAITLLVISGMLMLGVTNADSAMVSHRFT
                     DINPGASSANPYSFTRVGGDLYFTASDGQHGSEIWRLSDGRTEMVADVNPGKHGSEPG
                     YLTAFQGQLYFRAYSEATGVEMWRTDGETT"
BASE COUNT          574 a         1023 c         1026 g          487 t
ORIGIN      
        1 gagcggatat ccacgcgcac tcacggtctt caagggccag ctctatttcg cggccggcac
       61 ccccgcttcc gggcaggagc ttttccgtta cgacggcacc accgtcgagc agttcaccga
      121 catcaagccc ggcgcgggag atgcctgggc cgggttcggc aaggacacga tcctcgacgg
      181 ttacgcgtac ttcctggccg acgacggcgt tcacggcatg gaactctggc ggaccgacgg
      241 cgagaacacc gagctctggc tggacgcgac gccgggagcc gagggttcgt acttcttcag
      301 cctgaaggcg gcgggaggcc ggctctactt cgcgacccgg accgactacg gactgaatac
      361 ccgcgcgtgg cgctcggacg gcgtgaccaa tgatgtcctg ggcaatttcg aaatctggca
      421 gacgacccag gttggccaaa gggtctacct cctctcgggc cacagcctga tggtggtgac
      481 gggacccgat cgtcagcccg agtacaagag ccttcctttc agcgccgagg tggacaacct
      541 gaccgcgatc ggcaacagcc tctacttcac ggccaccgac cgcgtccacg ggtccgagcc
      601 ctggcggatc aaggacggca agctcgagct cctgggatcg gaggcgtatg acgaccaggg
      661 ttcgatcggc tggtacccgg ggcagacggg gccgttcgtc gagttcgacg gcgaggcgtt
      721 catcaccatg agcgactccc ggtacgggag tgaactctgg aaggtgcagg acggcaaggc
      781 ggtgctgttc aaggacatcc tgcccggccg ggattccgga ttgcccggcc tctataccca
      841 cttcggggac gaggtctatt tcatcgccga cgacggagta cacgggcatg agctctggca
      901 tcttttcgag gatgacgagg tcggcatcgt ggtcccggcg cagtcgccga agatccgcaa
      961 ccaagccacg agcatcagcc tcggttgccc ggcttcggag caggccgggc cctgccgggg
     1021 cagcctgaag atctcgcgca agggcaggac cctcggcagg ggaagcttcc ggatcggggc
     1081 gggtcacacc gggaagccgg tcgtgaaact cgggccccgg gtcgcggccg gtccggtttc
     1141 gcttgaggtc accgtgcgcg acgacgccgg gaattcgaaa acggtcagcc gcaagctgag
     1201 gctctcccga tgaggccgct ggcacggatt gcggccgtgg ccgtggccct gatctcggcc
     1261 ggtctgatcg cgggaacggc ccaggccgcg gtccgggccg aactcgcagc cgacgtcgac
     1321 ccgggcccga gcagctcccg gccgagcaaa ctgaccgcag tcggtgattc cgtttatttc
     1381 tacgccgacg gcggcccgga ccggcagggc ctctggcgca ccaatggcag gctgacccag
     1441 ctggtccggc cggtcggcga tcccgacctg cggatctact ggaccgagaa ggtcgccttc
     1501 cagggccggc tttacctgat gggatcggaa accgggaata actaccggct ctggcggacc
     1561 gtcccgggag gaggaaggat cgaaccggtt ccgggctggc cgaaagagtt gaaggcaggg
     1621 tctaagccga ccgtcgccaa cggcctgctc ttcttcaggg gcggtgacgg gctgcacaag
     1681 aacgaactct ggaagtacga cggcgaaagg gccagcctgg tcaaggacat caatccgggc
     1741 cccgccggtt cctttcccgg atacatcaca ggcctcggca accaggtgat cttcaacgcc
     1801 accgacgggg tccacggcgg cgagctctgg cgttccgacg gaaccgcttc agggacccgg
     1861 atgatcgggg aaatcaatcc gggcgccaag ggcaccaaca tggcctggct gaccagggtg
     1921 ggggattcgg tctatctcac cgcggacacg cccggccact ggctcgagcc ctggcgcacc
     1981 gacggagagc agctcggact cgtcgcagcc gaacacgaag gcccggaatg ggacaacatc
     2041 acctcgtttg cgggcctggg cggcaaggtc tacttcacgg cgaacaatga actctgggaa
     2101 agcgacgacg cctggccgga ggctgaggtt cacccgatcg cggacgaccc ggacttcccg
     2161 accacggcca agaacgcccc ggtcgcggcc gggaaccgga tcttcttcgc ctctttcgcc
     2221 ggggactgga ccggaaccat gcagagctgg cccagcttct acgacggcga gaagatcgtc
     2281 accctgaccg accccgaaga agaattcgac tacccctggg gctacccgca gttcttcaag
     2341 gagttcgggg gaagggtctt cttcgttggc ggagacaccc tccacggcac cgaactgcgg
     2401 gtcgtcagcg gccgtcacta ccggctgttc gcggacatcc tcgaagggga cagcagcagc
     2461 cggccgcgcg ggttcgcccg gtcgaagcac ttcatgtact tcgttgccga tgacggcatc
     2521 cacggccgcg agatctggcg cctgcgccgg accgcgggct aaagtccccg gcgcccggcc
     2581 cggcgggtcg ctcgcctgcc ccggaaacgg ggcttcgctg aagttttccc gggcccgcgt
     2641 aagaaaccgg ccggttcctc gttacacgga tgaaagggct tttcgaagcc gcaatcacca
     2701 agagaggaat cacgatgtac ccagaaattc accggcttcg attcgtactg gcggccgctt
     2761 cggcgatcac cctgctggtc atctctggca tgctgatgct gggtgtcacc aacgccgact
     2821 cggcgatggt ctcgcaccgc ttcaccgaca tcaatcccgg ggccagcagc gccaacccgt
     2881 acagcttcac ccgggtcggc ggcgacctct acttcacggc cagcgacggc cagcacggca
     2941 gcgagatctg gcgtctgagc gatggcagga ccgagatggt cgccgatgtc aaccccggca
     3001 agcacggctc cgaaccgggc tacctgaccg cgttccaggg ccagctctac ttccgggcct
     3061 actccgaagc gaccggggtc gagatgtggc gaaccgacgg cgagacgacc
//
LOCUS       DAOQMF010000133         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17410_length_3102_cov_3.559567, whole genome shotgun sequence.
ACCESSION   DAOQMF010000133 DAOQMF010000000
VERSION     DAOQMF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17410_length_3102_cov_3.559567"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLV77_03190"
     CDS             complement(<1..519)
                     /locus_tag="PLV77_03190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC14883.1"
                     /translation="MAGSLSSFARKGMLKPKYARLFWRYLWRRLLTGSGHRWETDGPV
                     FFGRGLHLQTGRRARIRFGRWVWIGDGTKIRCHEGEVEIGPKTVFGQECTISAYRRVR
                     IGAECVIADRTMFIDFDHGVVDVEKPIRDQGIYMEDVIIGSNVWIGYGAAILRGVRVG
                     DNSVIGTNSVVTR"
     gene            569..1534
                     /gene="meaB"
                     /locus_tag="PLV77_03195"
     CDS             569..1534
                     /gene="meaB"
                     /locus_tag="PLV77_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNC14884.1"
                     /translation="MNDAPITMSPLAERLLDGDKRALARAITLIESDDPAGWELVREV
                     YPKTGKARIIGFTGPPGVGKSTLIGALTKELRKEDRQVAVLSIDPSSPFTQGALLGDR
                     IRLTEHFLDQGVFIRSMASRGALGGLSEAALQAALVMDASGKDDVLVETVGVGQGEID
                     IVDHADTIVLALMPGSGDSIQALKAGVMEIPDIIVVNKSDHPMTDTMIREVRGALSLA
                     HTPGGWRVPILRTEAARGEGIEELAAKVAEHRAFIENEGTLAERRARNLRSEVLGLAT
                     ARLRRRLEAAIEDDPRVVELLNRVVSRELDPASAAAELLEGEIDE"
     gene            1527..1928
                     /locus_tag="PLV77_03200"
     CDS             1527..1928
                     /locus_tag="PLV77_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14885.1"
                     /translation="MSEKSSPQPSLEDIKAWEGYRLDDMTGQGVARVEGLFVDGESGQ
                     PTWVLVKLGRFGKVVPISIRECAAAAGRVWTPYDREVIRNAPAVDPALPLNREQEQQV
                     LEYYGIPETVGRGAELKDRPAGSTTANPITT"
     gene            2289..2933
                     /locus_tag="PLV77_03205"
     CDS             2289..2933
                     /locus_tag="PLV77_03205"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC14886.1"
                     /translation="MTIHGRRIEIIRALRELQNPRIPDEGRRDLAARDAILDSTVELV
                     LSEGWEALTIEGIANRARVSKQTIYRWWKSKGAVLMDAVLANGFKWPAIPDTGDFEAD
                     LKGGLRSLVGEFSDASFDSLLRALLIGAQEDGKLSAELDRQVRSWITAEFAFLIESAR
                     QEGRIEAHADPELIIELLYGAVFTRWLLRTGSLDFRFADAVAEMTARALTPGRS"
BASE COUNT          560 a          975 c         1038 g          529 t
ORIGIN      
        1 cctggtgact accgagttcg ttccgatcac cgagttgtcg ccgaccctga ccccgcgcag
       61 gatcgcggcg ccgtagccga tccacacgtt cgaaccgatg atcacgtcct ccatgtagat
      121 gccctggtcg cggatcggct tttcgacgtc gacgaccccg tgatcgaagt cgatgaacat
      181 ggtccggtcg gcgatcacgc attcggcccc gattcggacc cggcgatagg ccgagatcgt
      241 gcattcctgg ccgaagacgg ttttcggacc gatctcgacc tcgccttcgt gacaacggat
      301 cttggtgccg tcaccgatcc agacccaacg gccgaacctg atccgggccc tgcgtccggt
      361 ctggagatga agcccccggc cgaagaagac cgggccgtcg gtttcccagc ggtgccccga
      421 cccggtcagg agccggcgcc agaggtaacg ccagaagagc cgggcgtact tgggtttcag
      481 catgcccttg cgggcgaagg atgagagtga gccagccaaa ccggccgtga ctctaaccgg
      541 agccgcaggt tctagactgg ccgggccgat gaatgatgcc ccgatcacca tgagccccct
      601 ggccgaacgc ctgctcgatg gcgacaagcg ggccctcgcc cgggcgatca cgctgatcga
      661 atccgatgac ccggccgggt gggaactggt ccgcgaggtc tacccgaaga ccggaaaggc
      721 caggatcatc gggttcaccg gtccgcccgg ggtcggcaag agcactttga tcggcgccct
      781 caccaaggag ctccgcaagg aggaccgcca ggttgcggtc ctttcgatcg acccgtcgag
      841 cccgttcacc cagggtgccc tgctcggtga ccgaatccgc ctgacggagc acttcctcga
      901 ccagggtgtg ttcatccggt cgatggcatc ccgcggggcg ctcggcgggc tttccgaggc
      961 cgcgcttcag gctgccctgg tcatggacgc ttccggcaag gacgacgtcc tggtcgagac
     1021 ggtcggggtg gggcaggggg aaatcgacat cgtcgaccat gccgacacga tcgtcctcgc
     1081 cctgatgccc ggctccggtg attcgattca ggcgctgaag gccggtgtca tggagatccc
     1141 ggacatcatc gtggtcaaca aatccgacca cccgatgacc gacacgatga ttcgcgaggt
     1201 gcgcggcgcg ctctcgctcg cccacacacc gggtggctgg cgggtgccga ttctccgcac
     1261 cgaggcggct cgcggcgagg gaatcgaaga acttgccgcc aaagtggcgg aacaccgggc
     1321 cttcatcgag aatgaaggga cgctggccga aaggcgggcc cgcaatctgc gctcggaggt
     1381 gctggggctg gccacggcca ggttgcgtcg gcggctcgaa gcggcaatcg aagatgatcc
     1441 ccgtgtggtg gagttgttga accgagtggt cagccgggaa ctggacccgg cgtcggccgc
     1501 ggccgaactg ctggaaggag aaatcgatga gtgagaagtc ttccccgcaa ccgagtctcg
     1561 aggatatcaa ggcctgggag ggttaccggc tggatgacat gaccggccag ggcgtggccc
     1621 gggtcgaagg tctgttcgtc gacggcgagt ccggccagcc gacctgggtg ctggtcaaac
     1681 tgggccgctt cggcaaggtg gtcccgatct cgattcgcga atgcgcggct gccgccggcc
     1741 gggtctggac tccctacgac cgcgaagtca ttcgcaacgc gcccgcggtc gaccccgccc
     1801 tcccgctcaa tcgtgaacag gagcagcagg tactcgagta ctacggaatc ccggaaacgg
     1861 tgggccgcgg agccgaactc aaggaccgcc ccgccggctc aaccaccgca aacccgatca
     1921 ccacctgacc cgaaccccaa gcaatcgagg ccaggcaggc ccggaagaca accgaggtca
     1981 tcaggcccgg aaagcaatcg aggccaggca ggtcgggaaa ccaaccgagg ccccaggcag
     2041 gcccggaaat cccgtggcga gacgcaacgt tgcgtctcgt tgagatttga cgagttgtca
     2101 aatcctcggg ctgccgaacc ccgatcacca cgttttgtca agtccccgcg agacgccacg
     2161 ttgcgtctcg ccagtgtttt tttcgatgtg ttgcgggccc gaccggttgg gtccggtccg
     2221 accgggtccc gtcgggtcgg gttggtctga cgggggagag ttttgcgttt cggttactga
     2281 agtccgggat gacaatccac ggtcggagaa tcgagatcat cagggcactt cgcgaacttc
     2341 agaacccgcg gatccccgac gaaggccgac gcgaccttgc tgcccgtgac gcgattctcg
     2401 actccacggt ggagctcgtt ctttccgaag gctgggaggc tctgacgatc gaaggcatcg
     2461 ccaaccgggc acgggtatcc aaacaaacca tctaccggtg gtggaagtca aagggcgccg
     2521 tgctgatgga tgccgtactc gcgaatggct tcaagtggcc ggcgattccg gatacgggtg
     2581 atttcgaagc cgacctcaaa ggaggcctgc gtagcctggt cggtgagttc tccgacgcca
     2641 gcttcgattc cctgctgcgt gccttgttga tcggcgccca ggaggacggg aagctctccg
     2701 ctgaacttga ccggcaggtt cggtcctgga tcaccgctga atttgcgttc ttgatcgagt
     2761 cggcccgcca ggaggggcga atcgaagctc acgcggaccc ggagctgata atcgaattgc
     2821 tctacggggc cgtgttcacg cgatggctgc tgcggaccgg cagcctcgac ttcaggttcg
     2881 cggacgcggt ggcggagatg accgcccggg ctttgacacc cgggcggtca tgaaccaaac
     2941 ggatctggtc gactcaggac ctggtcgact caggccgcgg tcggctcagg ccctggtcgg
     3001 gtcaggacct ggtcgggtta gtggccgtgg tcgtggccgg cttcgccggt gctgaggctg
     3061 gcggccctgg agatcagggc ggatgaaccg gggcccggcg tg
//
LOCUS       DAOQMF010000134         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17476_length_3096_cov_2.851694, whole genome shotgun sequence.
ACCESSION   DAOQMF010000134 DAOQMF010000000
VERSION     DAOQMF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17476_length_3096_cov_2.851694"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLV77_03210"
     CDS             <1..224
                     /locus_tag="PLV77_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14887.1"
                     /translation="ELFATLSASARVDSPFVAEDTASIPRGAGWCEPELVIEVRFTEW
                     TREGRLRNPVFVGFRPDKSPREVVREEPA"
     gene            221..1177
                     /gene="ligD"
                     /locus_tag="PLV77_03215"
     CDS             221..1177
                     /gene="ligD"
                     /locus_tag="PLV77_03215"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNC14888.1"
                     /translation="MTFGWKRSERLTSAVTGGPGKASIEIDGRELRLTNLDRVLYPET
                     GFTKGDLIDYYAAVAPLLLPHLRDRPVTMRRFPDGVESEGFWEKHCPDHRPGWVKTAS
                     IESSSSSKGRVDYCLVNDVATLVWMANLGCIELHVSLARARRRKTPDFMVFDLDPGPP
                     ADILDCAETALLIHDTLAGQDLVSLAKVSGSKGIQVYVPLNGRASYDRTGGFAHSLAR
                     AFEQELPDQIVSRMTKSLRKGKILIDWSQNSEHKTTVSVYSMRARSSPSVSMPVGWER
                     LEQALDRSDDSLLRFGPAEVLRRISGTDPFGAILELKQKLPG"
     gene            complement(1238..1660)
                     /locus_tag="PLV77_03220"
     CDS             complement(1238..1660)
                     /locus_tag="PLV77_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC14889.1"
                     /translation="MSKTAREVMTGGAECIGESESITRAATRMAELDVGSMPICGEDD
                     RLKGMLTDRDIVVKVIAEGKDPDEVTAGEFGQGKPVTVGADDSVEVAIETMKEHAVRR
                     LPVIDGHELIGIVSQGDLACEVSEKAAGDLVEAISSAP"
     gene            1842..2057
                     /locus_tag="PLV77_03225"
     CDS             1842..2057
                     /locus_tag="PLV77_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14890.1"
                     /translation="MVKVGIAIGLVFLVAALSVFGSPFFAVLLLVPAGILAFAFLGGA
                     VVAEETGPEDSGKEPGPPKTSEAGQQN"
     gene            2130..2840
                     /locus_tag="PLV77_03230"
     CDS             2130..2840
                     /locus_tag="PLV77_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14891.1"
                     /translation="MIEAIGWGAVAASSLVLGVLLAFARPWSDRLVGSVLAFGAGALI
                     SAVSFSLAGEGLDIGSPPSVGAGLAVGALAYYLASRFIDRPRPGERADSSASGRDLAL
                     GSFLDGIPEQLVLGIGIVSGSGISLALLMAIFVSNLPEGIGSAADMERAGASRAYVLR
                     LFILIAIACAIAAAVGYLLADFISNELQACLDGFAAGALLVMMIDSMIPEAREKAGNV
                     AGLLTVVGFAAATALSGA"
     gene            complement(2843..>3096)
                     /locus_tag="PLV77_03235"
     CDS             complement(2843..>3096)
                     /locus_tag="PLV77_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14892.1"
                     /translation="WGKNRAVGRGHYYVTSPPIPGEKNPEGAARIVVWKPVKCVPDFG
                     NREGKTIHIYKHARMLRPVSGGGRKWIDISAFAGAATCR"
BASE COUNT          478 a         1031 c          985 g          602 t
ORIGIN      
        1 ccgaactctt cgcgaccctt tctgcgtcgg cccgcgtcga ctccccgttc gtcgcggaag
       61 acactgcctc gataccccgg ggcgccggct ggtgtgaacc ggaactggtg atcgaggtca
      121 ggttcaccga gtggacccgc gaaggacgac tgcggaatcc ggtcttcgtc gggttcaggc
      181 cggacaagtc gccacgggaa gttgtccggg aggagccggc atgaccttcg gctggaagcg
      241 cagcgaacga ctcacctcgg cggtcaccgg gggcccgggc aaggcatcga tcgagatcga
      301 cggcagggaa ctcaggctga cgaatctcga ccgggtgctc tacccggaga ccggtttcac
      361 aaagggcgac ctgatcgact actacgccgc cgtggccccc ttgctcctcc cccatctccg
      421 cgaccgtccc gtcaccatgc gtaggttccc cgacggggtc gaaagcgaag gcttctggga
      481 gaagcattgt cccgaccatc gccccggctg ggtcaagact gcctcgatcg agagcagctc
      541 atcctcgaag gggcgggttg actactgcct ggtcaacgac gtcgccaccc tcgtctggat
      601 ggcgaacctg ggatgcatcg aactccatgt ttcactggcc cgggccaggc gccggaagac
      661 gccggacttc atggtcttcg acctcgaccc gggcccgccg gctgacatcc tcgattgtgc
      721 cgagactgcc ttgctgatcc acgacacgct cgccggccag gatctcgtct cgctggcgaa
      781 ggtctcgggc tcaaagggaa tccaggttta cgttccgctc aacgggcggg ccagctacga
      841 ccggaccgga ggtttcgctc attcactggc ccgggccttc gaacaggaat tgccggacca
      901 gatcgtttcg cggatgacga aaagcctgcg caagggaaag atcctgatcg actggagcca
      961 gaactccgag cacaagacca ccgtttcggt gtactccatg cgggccagga gcagcccgtc
     1021 ggtttcgatg cccgtcggct gggagcgact cgagcaggcg ctcgaccgct cggacgactc
     1081 gctgctccgc ttcgggcccg cggaagtgct tcgcaggata agcggcaccg atccgttcgg
     1141 cgccatcctc gagctaaagc agaagctgcc cggctgaccc gggagaggga gtcaaggatt
     1201 ggggcgccgc ccttgccacg gcgacgcccc gggcgggtca gggtgcggag gaaatcgctt
     1261 ccacgagatc cccggccgcc ttctcggaga cctcacaggc gaggtcaccc tgactcacga
     1321 ttccgatcag ttcatgtccg tcgatcaccg ggagccgcct taccgcgtgc tccttcatgg
     1381 tctcgatcgc gacttcaacc gagtcgtcgg ctccgacggt gacgggctta ccctgaccaa
     1441 actcgccggc ggtcacctcg tcgggatcct ttccttccgc gatcaccttc acgacgatgt
     1501 cgcggtcggt cagcattccc ttcaggcggt cgtcttcgcc gcaaatcggc atcgatccca
     1561 catcaagttc ggccatccgc gtggcggcgc gagtgatgct ctccgactcg ccgatgcatt
     1621 cggcaccgcc ggtcattact tcccgtgctg tcttgctcat ctcctgcctc ctgtctgggt
     1681 ggtttgcccc tccgccgacc gctgtggcgg agttcaacta gaggcgtacc cccagccgga
     1741 gcgtccaaac tcaaagattg ggagatccgg tgtcgggtac ctgccgaagc gttcccgaaa
     1801 gttggccggt cgcgatttcg cggcgggccg gaaaggcgtc gatggtcaaa gttggcatcg
     1861 ctattggact cgttttcctc gtcgcggctc tctccgtatt cggatcgccg ttcttcgcag
     1921 tcctgcttct ggtcccggcc ggcattctcg cctttgcctt cctgggcgga gcggtagtgg
     1981 ccgaggagac cggtcccgaa gattccggga aagagccggg tccgccgaag acctccgagg
     2041 caggccagca gaactgaccc cgggcccggg ttcggtatcc gccgcacccg tctagagtcc
     2101 ccgccgatgg ccgtggcgga gactttccga tgatcgaggc gatcggctgg ggtgccgttg
     2161 ccgcgtcttc tctggttctc ggcgttctgc tcgcgttcgc gcggccctgg tccgaccgcc
     2221 tggtcgggtc cgtgcttgcc tttggcgctg gtgcgctgat cagcgcggtc agtttcagcc
     2281 tggccggcga gggcctggac atcggcagcc cgccatcggt cggcgccggt ctcgcggttg
     2341 gagcgcttgc ctactacctt gcgagccgtt tcatcgaccg gccccggccc ggcgaaaggg
     2401 ccgattcgtc agccagcggc cgggaccttg ccctcgggtc cttcctcgac gggattccgg
     2461 aacagctggt gctgggtatc ggcatcgttt ccggatccgg gatcagcctg gccctgctga
     2521 tggcgatctt cgtttcaaac ctgccggagg gaatcggttc cgccgccgac atggagcggg
     2581 cgggtgcctc gcgggcgtac gtactccggc tgttcatcct gatcgcgatt gcctgcgcga
     2641 tcgctgcggc ggtcggttac ctgctcgccg atttcatctc gaacgaactc caggcctgcc
     2701 tggatggttt cgccgccggc gccctgctgg tgatgatgat cgactcgatg attcccgaag
     2761 caagagaaaa ggccggcaat gttgccggcc ttctcacggt tgtcggtttt gccgcagcga
     2821 ctgcgctgag tggcgcctga acctacctgc aggtggccgc gccggcgaag gcggagatgt
     2881 cgatccactt gcgccctccc ccggaaacgg gtcgcagcat cctcgcgtgc ttatagatgt
     2941 gaatcgtctt gccctcgcga ttgccgaaat ccgggacgca cttgaccggc ttccagacga
     3001 cgatccgcgc cgcgccttcc gggttctttt caccggggat cggcggcgag gtcacgtagt
     3061 agtggccgcg gccgaccgcg cggttcttgc cccagc
//
LOCUS       DAOQMF010000135         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17658_length_3079_cov_4.121032, whole genome shotgun sequence.
ACCESSION   DAOQMF010000135 DAOQMF010000000
VERSION     DAOQMF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17658_length_3079_cov_4.121032"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLV77_03240"
     CDS             complement(<1..596)
                     /locus_tag="PLV77_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC14893.1"
                     /translation="MAASPPPRRQQTRAKDLERYASLFARRTRVMRSSAMRDMMEITA
                     RPEVISLAGGLPDTSTFPPEAFAAEMHRIEKTAVAEVLQYGPTEGSWLVREQIVEVMA
                     AEGTRVDALDITVTTGGQQGIDLITKTLIDPGDVIICDAPTYPGAIPTFCSYEADVRQ
                     IEMDADGMRVDLLEEMIAKLEAEGRKPKFIYSIPTFQNP"
     gene            668..1042
                     /locus_tag="PLV77_03245"
     CDS             668..1042
                     /locus_tag="PLV77_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14894.1"
                     /translation="MIAGTTGLEGSARGRMAILAGIIGAVLGYLGTSILDGAPVIGAL
                     VGAIAAVLSTAVVSDVIAGAQRRGGTAALAVITVFAALVIAIICLLFPFFAIIPAAFL
                     VFLAWRRRQQADRKHAGLRVLR"
     gene            1058..2689
                     /locus_tag="PLV77_03250"
     CDS             1058..2689
                     /locus_tag="PLV77_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNC14895.1"
                     /translation="MGKKLVLAYVDSLRTDMLKQTIEESRAPTFRRLLKRGTLIEDCV
                     SSFPSVTPVASAEMVTGAQADRHWISGMNWFHRAEQRYVEYGSSLEATRAFGLFRSLH
                     DLVYNMNLSHLSPSVETVFETLDDADIRTASTPFLIYRGRHRHEVNIAGITGKAIEAG
                     LLKFDYGTWGPRELFYGDLYTSMETKCKPSSIPGKRDMYSACCGAELISRDAFDFMLL
                     AFPDNDNYSHRHGPLASVRSIERADYCMAEVIRAAGGVEPFLQDHALILLADHAHTAV
                     EHRYDIIEWMSRKWKVLPPSSEEPAEAELAVSPTSRAAHIYLLAEQDNPIRHRRVREH
                     LVGLEGTELVSWTERDGQPVVRSTRPGHLEPGNVAVVERDGRRLDFFPLSSGANGDGQ
                     GEVPQKQPRDGAKRAAPSPGEGRETVTDRRGRQWSISGNIEALGASVEEGRIETPEYP
                     DGLGRLWSALVAPHGGDIIVSAAPGYECVDWGGAAHVPGGSHGSLRKEDSEGPLLFVG
                     CGPDSPDPETAARHRSRPQWGLQDVAPIIREFFGT"
     gene            complement(2733..>3079)
                     /locus_tag="PLV77_03255"
     CDS             complement(2733..>3079)
                     /locus_tag="PLV77_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HNC14896.1"
                     /translation="VVVDFHAEVTSEKVAMGWYLDGRVAAVFGTHTHVPTADGRVLPG
                     GTAFISDVGMTGARDSVLGVKKEQAIEKMRTDMPIRFESATDDVWVMGAAVEIDENGL
                     ATGFKQILEPSS"
BASE COUNT          493 a         1073 c          988 g          525 t
ORIGIN      
        1 ggattctgaa aagtgggaat cgagtagatg aatttcggct tgcggccctc ggcctcgagt
       61 ttcgcgatca tctcctcgag caggtcgacc cgcatcccgt cggcgtccat ttcaatctgc
      121 cggacatcgg cttcgtagga gcagaaggtc gggatcgccc cggggtaggt cggggcgtcg
      181 cagatgatca cgtcgccggg gtcgatcagg gtcttggtga tcaggtcgat cccctgctgg
      241 cccccggtgg tgacggtgat gtccagcgcg tcgacccggg ttccctctgc cgccatcacc
      301 tcgacgatct gctcgcggac cagccaggat ccttcggtcg gcccgtactg gagcacctcg
      361 gcgaccgcgg tcttctcgat ccggtgcatt tcggcggcga aggcctccgg ggggaaggtg
      421 gaagtgtccg gcaggccgcc ggcgagggaa atcacctcgg gacgggcggt gatctccatc
      481 atgtcccgca tcgccgaaga gcgcatcacc cgggttcgcc tggcgaaaag agaggcgtac
      541 cgctcaaggt ctttggcccg ggtctgctga cgccggggag gtggtgaggc cgccatcctt
      601 ctattctgcc ggtatcgaca ctttctcgac aatctgcctc gccgccggaa tcggcattgc
      661 gaccggaatg atcgccggca caaccggcct ggaaggttcc gcccggggcc gcatggcgat
      721 cctcgccggg atcatcggcg cggtcctcgg ctacctcggt acctcgattc tcgacggcgc
      781 cccggtcatc ggtgccctgg tcggcgcgat tgcggcggtg ctgagcaccg ctgtcgtttc
      841 cgacgtgatc gccggggccc agcggcgggg cggcacggca gccctggcag tgatcaccgt
      901 cttcgcggcc ctggtgatcg cgatcatctg cctgctcttc ccgttcttcg ccatcatccc
      961 ggcagcgttc ctcgtcttcc tcgcctggcg ccgacgccag caggccgacc gcaagcacgc
     1021 cggtctccgc gtcctccgct agccgcccgg ttccgccatg ggaaaaaagc tggtcctcgc
     1081 ctatgtcgat tcgcttcgaa ccgacatgct caaacagacc atcgaggaga gccgcgcgcc
     1141 caccttcagg cgcctgctga agcgcggcac cctgatcgag gactgcgttt catccttccc
     1201 ctcggtaacc cccgtggctt cggccgagat ggtcaccggg gcccaggcgg accgacactg
     1261 gatcagcggc atgaactggt ttcaccgggc cgagcagcga tacgtggagt acgggtcatc
     1321 gctcgaggcg acccgggcct tcggcctgtt ccgctcgctg catgacctcg tctacaacat
     1381 gaacctgagc catctcagcc cctcggtcga aacggttttc gaaaccctcg atgacgccga
     1441 catccggacc gcgtccacgc cgtttctgat ctaccgcggc cgccatcgcc acgaggtgaa
     1501 catcgccggg atcaccggca aggcgatcga ggccgggctg ctcaagttcg attacggcac
     1561 ctgggggccg cgggaactct tctacggcga cctctacacc tcgatggaaa cgaagtgcaa
     1621 gccgagctcg atccccggca agcgtgacat gtactcggcc tgctgcgggg ccgagctgat
     1681 ctcccgggac gccttcgact tcatgctgct ggccttcccc gacaacgaca actactccca
     1741 ccggcacggc ccgctggcct cggtccgctc gatcgaaaga gccgactact gcatggccga
     1801 ggtgatccgc gcggccggcg gggtcgagcc tttcctccag gaccacgccc tgatcctgct
     1861 ggccgaccat gcccacaccg cggtcgagca ccgttacgac atcatcgagt ggatgagccg
     1921 caagtggaag gttctgccgc cttcctccga ggagccggcc gaggccgagc tggcggtcag
     1981 tccgacttcc cgcgccgccc acatatatct gctggccgag caggacaacc cgatccggca
     2041 ccggcgggtt cgcgagcatc tggtcggcct cgaagggacc gaactggtca gctggaccga
     2101 aagggacggc cagccggtcg ttcgctcgac ccgccccggc cacctcgaac cgggcaacgt
     2161 cgcggtcgtg gaaagggacg gtcgccggct cgacttcttc cccctgagtt cgggggcaaa
     2221 tggcgacggg cagggagagg tgccgcaaaa acagccccgt gacggggcaa aacgtgcggc
     2281 accttccccg ggtgagggcc gtgaaacggt gaccgaccgg cgcgggcgac agtggtcgat
     2341 cagcggcaac atcgaggctc tcggggcctc ggtcgaggag ggccggatcg agacgcccga
     2401 atacccggac gggctcggcc ggctctggtc agccctcgtc gccccccatg gcggagacat
     2461 catcgtctcc gccgcgcccg gctacgaatg cgtcgactgg ggcggagccg cccacgtgcc
     2521 cggcggcagc cacggttcgc tccgcaagga ggattccgaa ggcccgctgc tcttcgtcgg
     2581 ctgcggcccc gactcaccgg acccggaaac cgccgcccgc caccggtccc gcccccaatg
     2641 gggcctccag gatgtggccc cgataatcag ggaattcttc ggcacctgag aggagccggg
     2701 tccggccccc tcccggagac atccgccctc cgctaggacg agggttcgag gatttgcttg
     2761 aacccggttg cgaggccgtt ttcgtcgatc tcgaccgcgg cgcccattac ccagacatca
     2821 tcggtggcgg attcgaagcg gatcggcatg tcggttcgca tcttctcgat cgcctgttcc
     2881 ttcttcactc cgaggaccga atcgcgggcg ccggtcatgc cgacgtccga gatgaaggcc
     2941 gtgcctccgg gaagcacccg cccgtcggcg gtcggcacgt gggtgtgggt gccgaacacg
     3001 gcggcgaccc ggccgtccag gtaccagccc atcgccacct tttcgctggt cacctcggcg
     3061 tggaagtcaa cgaccaccg
//
LOCUS       DAOQMF010000136         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17660_length_3079_cov_3.763558, whole genome shotgun sequence.
ACCESSION   DAOQMF010000136 DAOQMF010000000
VERSION     DAOQMF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17660_length_3079_cov_3.763558"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..939)
                     /gene="rapZ"
                     /locus_tag="PLV77_03260"
     CDS             complement(25..939)
                     /gene="rapZ"
                     /locus_tag="PLV77_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNC14897.1"
                     /translation="MAAAEPGEDRSEDEGQREDPVEMVVITGLSGAGKSEAIAAFEDA
                     GYFCVDNLPPQMIGALGELFRLPGSGVTKAAVVSDARGGEYFEQLNQVLDDLEGDGIK
                     PKLIFLEAHEEILTDRYKETRRRHPSAPEGRITEGIEREREILAPLRARADVVIDTSG
                     ESASTLRRRISEDLIDTGSSSLMVVNLLTFGFKNGPPRDADITLDVRFLPNPHYVDDL
                     RPLTGLDQEVRDYVEAGTMAGEFYGRLLPLLEFLIPAYVAEGKSHLTIAIGCTGGRHR
                     SITVAERIWREFSTRDDVKVRVTHRDIA"
     gene            complement(945..2843)
                     /gene="uvrC"
                     /locus_tag="PLV77_03265"
     CDS             complement(945..2843)
                     /gene="uvrC"
                     /locus_tag="PLV77_03265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNC14898.1"
                     /translation="MAESTGKQQSKEPDREARIVAERRSLPDVPGVYLFRDRDDEVIY
                     VGKAVSIRKRVASHFSGGNAGMTRLIERIEFLATESESEALIAEQSFIKRYRPRFNIK
                     LRDDKSYPYVAVSLDEDYPRVYFTRERHRPGRAYFGPFSSARRVRDTLDLLGKLFQYR
                     TCEGKEPGRRSGSPCLDYFIKRCGAPCVDYISQADYRANIDRVIDFLSGRYDQIERDL
                     DTRMKEAAAAQDFEAAAVFRDRLASVQSLFERQRISGSTVGTADLIGVATEGTDANAQ
                     VFQVRDGILAERQSFYLENPAERGIDEVTAEFIEQYYGASPAVPKRVVLGPYMSEKLA
                     EVETFLAGRRANRATEVRIAERGDTRRLREMAERNAKLALDQDRLQSEHRQQRRIEAL
                     SQLGDALGMDELPVRIEGFDISNLGGQHTVASMVVFEGGRPKKSDYRRFRVRGEEGRP
                     GPDDFASMEEVLGRRIAQYRQQADLSPHDRDRDESFATLPSVIMIDGGKGQLSAGVRA
                     LREFIDLGVTVVSLAKREEEVFTPGSSLPLDLKADSEANLLLQRVRDEAHRFAITHHR
                     GRRDRAMTASVLDGIRGVGPNRKRLLIRHFGSPDRLLEASREEIEAVPGLPGKVAREI
                     HDQLNRIG"
     gene            2891..>3079
                     /locus_tag="PLV77_03270"
     CDS             2891..>3079
                     /locus_tag="PLV77_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14899.1"
                     /translation="MAAETLTPPELKTIDIAIDGEIGTMTLNRPETFNAMSPDLIEDL
                     ATAFAWLADQAPLRGLIIT"
BASE COUNT          490 a         1035 c         1010 g          544 t
ORIGIN      
        1 caacaaccct acggggttgg ggaatcaggc aatgtcccgg tgcgtgaccc ggaccttcac
       61 gtcgtcgcgg gtcgagaact cgcgccagat ccgttccgcc acggtgatcg accggtgccg
      121 gccccccgtg cagccgatcg cgatcgtgag gtggctcttg ccctccgcga cataggccgg
      181 gatcaggaac tcgagcaggg gcagcagccg gccgtagaac tcgccggcca tggtgccggc
      241 ttcaacgtag tcacggactt cctggtcgag accggtcagc ggcctcagat cgtccacgta
      301 gtgggggttc ggcagaaagc ggacgtcaag ggtgatgtcg gcatcccgtg gcggcccgtt
      361 cttgaagccg aaagtgagca ggttgaccac catcagcgaa ctggaccccg tgtcaatcag
      421 atcctccgag atccggcgac gcagggtcga ggccgattcg ccggaagtgt cgatcaccac
      481 atcggcccgg gcccggagcg gggccaggat ctccctttcg cgttcgatcc cttcggtgat
      541 ccggccttcc ggggccgagg gatggcggcg gcgggtttcc ttgtaccggt cggtgaggat
      601 ctcctcgtgg gcctcgagga agatcagctt cggcttgatc ccgtcgcctt ccaggtcgtc
      661 gaggacctgg ttcagctgct cgaagtactc gccgccgcgg gcatccgaga ccacggcggc
      721 cttcgtcact ccgctgcccg gcaggcggaa gagctcgccg agcgcgccaa tcatctgggg
      781 cggcaggttg tccacgcaga agtagcccgc gtcctcgaag gcggcgatcg cctccgactt
      841 gcctgccccg gagagaccgg tgatcaccac catctcgacc ggatcctctc tttgaccctc
      901 gtcttcgctt cggtcctcgc ccggctctgc ggccgccatg ggcactatcc gatcctgttc
      961 agttggtcgt gaatctcgcg ggccacctta ccgggcagcc cgggcaccgc ttcgatctct
     1021 tcgcggctgg cctcgagcag gcggtcgggc gagccgaaat gcctgatgag caatcgcttg
     1081 cggttcggtc cgaccccgcg gatcccgtcc aggaccgagg ccgtcatggc acggtcgcga
     1141 cggccccggt gatgggtgat cgcgaagcgg tgggcttcgt cccggacccg ttgaagcagc
     1201 aggttcgctt ccgagtcggc cttgaggtcg agcggcaggg atgaacccgg ggtgaagacc
     1261 tcctcctcgc gcttggcgag gctgaccacg gtgacgccga gatcgatgaa ctcccggagg
     1321 gcccggaccc cggccgagag ctgacccttg ccgccgtcga tcatgatcac gctcggcagc
     1381 gtggcgaagc tttcgtcgcg gtcccggtca tgcggagaaa ggtccgcctg ctgccggtac
     1441 tgcgcgattc gccggcccag cacctcctcc atcgaggcga agtcgtccgg gcccggacgg
     1501 ccctcctccc cccgcacccg gaaccggcgg tagtcggatt tcttcgggcg gcccccctcg
     1561 aagaccacca tcgaggcgac cgtgtgctga ccgccgaggt tcgagatgtc gaagccctcg
     1621 atcctgaccg gcagctcatc catgcccagg gcatcgccga gctggctcag ggcctcgatc
     1681 cttcgctgct gccggtgctc gctctgaagc cggtcctggt ccagggccag cttcgcgttc
     1741 cgctccgcca tctcgcgaag ccggcgggta tcgccccgtt cggcgatcct cacctcggtt
     1801 gcccggttcg cccgccgccc ggccaggaag gtctcgacct cggccagctt ctccgacatg
     1861 taggggccga gcacgacccg cttcggcacc gccggcgagg ccccgtagta ctgctcgatg
     1921 aactcggcgg tgacctcgtc gatccctcgc tcggccggat tctcgaggta gaaggactgg
     1981 cgctcggcga ggatcccgtc gcggacctga aagacctggg cgttggcatc ggtcccctcg
     2041 gtcgcgacgc cgatcagatc agcggtgcca accgtggagc cggagatccg ctgtcgttcg
     2101 aacagcgact ggaccgaggc cagccggtcg cgaaagactg ccgcagcttc gaaatcctgg
     2161 gcggccgccg cctccttcat gcgggtgtcg aggtcccgct cgatctggtc gtaccggccc
     2221 gaaagaaagt cgatcacccg gtcgatgttg gcccggtagt cggcctggct tatgtagtcg
     2281 acgcagggag cgccacagcg cttgatgaag tagtcgaggc aggggctgcc tgacctgcgg
     2341 cccggttctt tgccttcgca ggtccggtac tggaagagct tgccgagcag gtcgagcgtg
     2401 tcccgcaccc ggcgggccga ggaaaacgga ccgaagtagg cccggcctgg ccggtgccgc
     2461 tcccgggtga agtagacccg cgggtaatcc tcgtcgaggc tgaccgcgac atagggatag
     2521 gacttgtcgt cccgcaactt gatgttgaag cggggccggt agcgcttgat gaagctctgt
     2581 tccgcaatca gggcctcgga ttccgattcg gtggcgagaa actcgattcg ctcgatcagc
     2641 ctggtcatcc cggcgttgcc gccgctgaaa tgcgaggcga cgcgcttgcg gatcgagact
     2701 gccttgccga cgtagatcac ttcgtcgtcg cggtcgcgga acaggtaaac ccccggcaca
     2761 tccggcagcg agcggcgctc ggcaacgatc cgcgcctcgc ggtccggttc ctttgattgc
     2821 tgtttgcctg tggattcggc cacaagcgaa gggtaagacc caagggggtt gggatacgct
     2881 ggcgccacct atggccgctg aaacgctcac tcccccggaa ctgaaaacca tcgacatcgc
     2941 gatcgacggc gagatcggga cgatgacctt gaaccgtccg gagaccttca acgctatgag
     3001 tcccgacctg atcgaggacc tggccacggc tttcgcctgg cttgccgacc aggccccgct
     3061 gcgcggactg atcatcacc
//
LOCUS       DAOQMF010000137         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17691_length_3075_cov_3.474172, whole genome shotgun sequence.
ACCESSION   DAOQMF010000137 DAOQMF010000000
VERSION     DAOQMF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17691_length_3075_cov_3.474172"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..641
                     /locus_tag="PLV77_03275"
     CDS             258..641
                     /locus_tag="PLV77_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC14900.1"
                     /translation="MSLSECRIAAVAAVSDLDRSRAFFEGTLGLSAVDSGAGGDEVVY
                     SCGEGTGLMVYPSPENAGKGTATLGCWAVDDLESEMKILKDRGIEFERYEGFDQDENG
                     ILDMGDGRVAWFTDPDGNIFAVAEG"
     gene            699..1286
                     /locus_tag="PLV77_03280"
     CDS             699..1286
                     /locus_tag="PLV77_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14901.1"
                     /translation="MKSWQPPLLLVVLIVPAALGFVLAGPGLGLSIAGLSLVGLVVVA
                     MLVIPRDPIGRTPDPELARRLLVVTTFPIEDAVSIQRVADEVRIGFDENDAEIRLLTP
                     ATNTFLRRWATDLERARDNAQRDLVVSVASLTLAGVDASASVGDEDLVQAVEDELVTF
                     DATEVFLLTRGGSGSAAAEQLRERLRPRFHHVDLR"
     gene            1297..2031
                     /locus_tag="PLV77_03285"
     CDS             1297..2031
                     /locus_tag="PLV77_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC14902.1"
                     /translation="MSAVIEVEGLIKNFGSTRALDGLDLKVEPGQVHGFLGPNGSGKS
                     TTIRILLGLISASGGSARLFGEDPWKNAVSLHRRLAYVPGDVNLWPNLSGGETIDMLG
                     SLRGGLDPGRRKELVERFELDLTKKASTYSKGNRQKVALIAALATDAELFIFDEPTSG
                     LDPLKEAVFQECVKEITGQGHTILLSSHILGQLEHLADTFTIIKDGRTVETGTLAHLR
                     EIAASAGRDPYSLSLEDLFLSIYANP"
     gene            2028..>3075
                     /locus_tag="PLV77_03290"
     CDS             2028..>3075
                     /locus_tag="PLV77_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14903.1"
                     /translation="MSGFAGTADAIRFALKRDRLILPIWVAVLIGWIAMEVISYKNLY
                     GTTAQLDALYRSVAGNPAMVAISGPTGGIRTLGGAITWDSLPTISVVSGVFAMFSVVR
                     HTRAEEEDGRTELLLAYGSGRLAPLAAALTVTLAALALASAGYVAVFAAEGLPVGPAT
                     LLSLSFFGFGAVIAGTTAVVCQVTSKARAARGLVGIVIGAAWLLRAIGDTGGGTLTWF
                     SPLGWAEQTRPFWEDRWWPLLLPLAAFAVTLALSGWLLVRRDIGGGLIQPRPGPARAH
                     KSLLHPLGFAFRIQRGSIIGWAVMLFAYGFAVGVIGDNIEQLLKTSSAFTEAFASASG
                     NLLDSYFASILTVIA"
BASE COUNT          480 a         1022 c         1008 g          565 t
ORIGIN      
        1 aactgcgctc ggtcatgacc gccaggactg cctgagcgcc gatcgcttcc gcctcgtttc
       61 gcagggcatc cccgagttca cgggtggccg ggttgcagat gaccaggact tcctcgtttg
      121 gccgcacccc catgcattcg tggacgacgg ttctgactgc cttgtcaaga tcgctcatag
      181 ggggaaacta gtggtcaggg agcctcggcg aggctagtgt gggtacaaag gcaaatcacc
      241 agtcccagga ggtagcgatg agcctgagtg aatgcaggat cgccgcggta gccgctgtgt
      301 cggatcttga ccggtcccgg gccttcttcg aaggcacgct cggcctgtcg gccgtggatt
      361 cgggcgccgg cggtgacgag gtcgtctaca gctgcggcga gggaaccggc ctgatggtct
      421 atccctcacc cgagaatgcc ggcaaaggca cggcaaccct cggttgctgg gcggttgacg
      481 atctcgaatc cgagatgaag atcctgaagg atcgcggcat cgagttcgaa cgctacgagg
      541 gcttcgatca ggacgagaac ggaatcctcg acatgggcga cggaagggtc gcctggttca
      601 ccgatccgga cggcaacatc ttcgctgtcg ccgaaggctg atcgggctgc gccgcgcccg
      661 gcccggatgg tttcgggccg gacgggtact ttccggccat gaagtcctgg cagccgccac
      721 ttctcctcgt cgtcctgatc gtgcccgccg cgctgggctt cgtgctggcg gggcccggcc
      781 tcggcctgtc catcgccggc ctctccctgg ttggcctggt cgtcgtggcg atgctcgtca
      841 tcccccggga tccgatcggc cggactcccg accccgaact cgcccggcga ctgctggtag
      901 tgaccacctt cccgatcgaa gacgcggttt cgatccagcg ggtggccgat gaagtccgga
      961 tcggatttga cgaaaacgac gccgaaatcc gcctgctgac cccggcgacc aacaccttcc
     1021 tgcgccgctg ggccaccgac ctcgagcggg cccgcgacaa cgcccagcgg gacctggtcg
     1081 tgtcggtcgc ctcgctcacg ctcgccggcg tcgacgcctc cgcctcggtc ggtgacgagg
     1141 atctcgttca ggccgtggaa gatgaactgg tcaccttcga cgcgaccgaa gtctttctgc
     1201 tgacccgggg cggttccggg tcagctgcgg ccgagcagct ccgcgagcgg ctgcgacccc
     1261 gcttccatca cgtcgacctg cggtagtttc cgggccatga gcgcggtcat tgaggtcgag
     1321 ggcctgatca agaacttcgg ttcgacgagg gcgctggacg gccttgacct caaggttgaa
     1381 ccgggtcagg tccacggatt cctcggaccg aacggctccg gcaagtccac cacgatccgg
     1441 atcctgctcg gcctgatcag cgcctccggc ggaagcgcaa ggctcttcgg cgaggacccc
     1501 tggaagaacg ccgtttccct gcaccggcgg ctcgcatacg tgcccggtga cgtgaacctt
     1561 tggccgaacc tgtcaggtgg cgaaacgatc gacatgctgg gctcgctgcg tggcgggctc
     1621 gaccccggga ggcgcaagga actggtcgaa cgcttcgagc tggatctgac caagaaggcc
     1681 tccacatatt cgaaggggaa ccggcagaaa gtggccctga tcgcggcgct cgccaccgat
     1741 gccgagctct tcatcttcga cgagccgacc tccggcctcg atccgctcaa ggaggcggtc
     1801 ttccaggaat gcgtgaagga gatcaccggc cagggccaca cgatcctcct ttccagccac
     1861 atcctcggtc agctggaaca cctggccgac actttcacga tcatcaagga tggccgcacg
     1921 gtcgagaccg gaacgctcgc gcacctgcgg gaaatcgccg ccagcgccgg ccgggatccg
     1981 tactcgctca gcctggaaga tctcttcctc agcatctatg cgaacccatg agcgggttcg
     2041 cgggaactgc tgatgcgatt cgcttcgcgc tgaagcggga ccgtctgatc ctgccgatct
     2101 gggtcgccgt gctgatcggc tggatcgcga tggaggtgat cagctacaag aacctttacg
     2161 ggacgacggc ccagctcgac gcgctttaca ggagcgttgc cggaaatccg gcgatggtcg
     2221 cgatttccgg tccgaccggc ggcattcgca cgctgggcgg ggcgatcacc tgggacagcc
     2281 ttccgacgat ctcggtggtc agtggcgtct tcgcgatgtt cagcgtggtt cgccacacca
     2341 gggccgagga ggaggacggc cggaccgagc tcctgctggc ctacggttcg gggcgtcttg
     2401 ccccgctcgc cgcggccctg accgtgacgc ttgccgccct ggcgctggcc tcggccggct
     2461 atgtcgcggt ctttgcggcg gagggtctcc cggtcgggcc ggcgaccctc ctgtcgcttt
     2521 ccttcttcgg cttcggggcc gtgatcgccg ggaccaccgc cgtggtctgc caggtgacga
     2581 gcaaggcaag ggcggcgcgg ggactggtcg ggatcgtgat cggtgcggcc tggcttctgc
     2641 gggcgatcgg ggacaccggc ggcggcacac tgacctggtt ttcgccgctc ggctgggctg
     2701 aacagaccag accgttctgg gaggaccgct ggtggccgct gcttttgccc ctcgccgcct
     2761 tcgcggtgac cctcgccctg tccggctggc tgctggtccg ccgggacatc ggtggcggat
     2821 tgatccagcc ccgtccggga ccggcccgcg cccacaaatc cctcctccac ccgcttggct
     2881 tcgccttccg cattcagcga ggatcgatca tcggctgggc ggtgatgctg ttcgcctacg
     2941 ggttcgcggt cggcgtgatc ggcgacaaca tcgagcagct gctcaaaacg agctccgcct
     3001 tcaccgaagc attcgcatcg gcgagcggaa acctcctcga ttcctatttc gcttcgatcc
     3061 tcaccgtgat cgccc
//
LOCUS       DAOQMF010000138         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17716_length_3073_cov_3.645129, whole genome shotgun sequence.
ACCESSION   DAOQMF010000138 DAOQMF010000000
VERSION     DAOQMF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17716_length_3073_cov_3.645129"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLV77_03295"
     CDS             <1..1080
                     /locus_tag="PLV77_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14904.1"
                     /translation="LAATVSLAFASSAGAAGESVTATTSISGMGGKLFKEKQVAARLN
                     IHAEVTTPDTSPQVLPMKNVKVTFPAGMAIRPNNSKTPVCSDSKLSNTSNLSNPSGVV
                     ASCSRSVVGSGTAAIYLAKVNLPTALITDPILIAFNAGYTGSGQPRIKIYGYSKTTNV
                     GILMNGTLKGSVLSMAVPVLSNDSAVKYFDLNFPGGRLDREDIGVHVHGLDPNYVQAR
                     CASSPLKTNAVFELGERTYPAGTDVGPTTRVASPETMQSCVGLAGHAKLKAKVVKKPR
                     AVRNGGRGFFRVYVRNYGTATAKGVYLFAPSVKKKVGWIAPGVARTIGIRARVRGRKG
                     HSRTIRFTVKGNGVSASAFARVRVK"
     gene            complement(1147..1962)
                     /locus_tag="PLV77_03300"
     CDS             complement(1147..1962)
                     /locus_tag="PLV77_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNC14905.1"
                     /translation="MKIFSWNVNGIRAVIRKGDFGSFVEKYDPDILCMQETKAEQGQA
                     EIDLPQYEEYWNSAGKKGYSGTAIFTRQKPIAVLNDFAAEISDEYDLHNDSYGDPRAE
                     GRVITAEYDHFFLVTTYVPNAKRDLSRLKLREKQWDPALLHHMKMLDAKKPVLVCGDM
                     NVAHTEDDLANPKANAGEHGFTDEERQGFQNFLDAGFLDTFRLFAEGNGHYSWWSYMS
                     KARERNAGWRIDYFLASERLRQAITGAEILGDVMGSDHCPVSVELDEKKLAKL"
     gene            2000..>3073
                     /locus_tag="PLV77_03305"
     CDS             2000..>3073
                     /locus_tag="PLV77_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14906.1"
                     /translation="MWQGRGWQGLATVIAIAISILVPATANAGEGGFTTGRLGPYQIR
                     FSDSPFGFSISRGGRYLLAGLGHSFERNGVRYATLGYTPQPAKELEPPDLSAAPDEEP
                     STQTSYVATRANGMWFGPDVYEARPRTDSPGGRRMRLTIWLRKDGTLGIRAGMMAGKA
                     GSVYLGFRSPQSEAFHGFGGRREGTDLRGSDIKSWVLDYRYPDPTTAYYSPVPGFISS
                     RGYGLLLKGDRISRWRMASDRPTAWRVSLPGGSMEMTLTSGGQARAMAGISRLTGRHR
                     LPPAWATGTVLSRAIGIAADSGGKYQSRVESDLEHLASADFPISAYAFEGWQALPGSF
                     VKATIRTLKEEGIHPVLYLRSFVS"
BASE COUNT          542 a          988 c          997 g          546 t
ORIGIN      
        1 ctggccgcca cggtctcgct cgccttcgct tccagcgccg gcgccgcggg tgaatcggtc
       61 accgcaacca ccagcatctc cggcatgggg ggcaagcttt tcaaggagaa gcaggttgca
      121 gccaggctca acattcatgc ggaggtgacg acaccggaca cctcgccgca ggtcctgccg
      181 atgaagaacg tgaaggtcac gttccccgca ggaatggcaa tccgcccgaa caattcgaag
      241 actcccgtct gctcggacag caagctcagc aacacttcga acctttcgaa tccttcgggc
      301 gtcgtcgcct cctgcagcag gtcggtcgtc ggcagcggta ccgcggcgat ctacctcgcc
      361 aaggtcaacc tgccgacagc gctcatcacc gaccccatcc tgattgcttt caacgccggt
      421 tacacgggca gtggtcagcc caggatcaag atctacggct actcgaagac caccaacgtc
      481 ggcattctta tgaacggcac cctgaagggg agcgtcctca gcatggccgt tccggtgctg
      541 agcaatgact cggccgtcaa gtactttgat ctgaacttcc ccggtggcag acttgatcgc
      601 gaagacatcg gcgtccacgt tcacggtctc gacccgaact atgtccaggc caggtgtgcc
      661 agcagcccgc tgaagaccaa cgccgtcttc gagctcggcg agcgcaccta cccggctggc
      721 accgacgtcg gtccgaccac ccgggtcgct tcccccgaga ccatgcagag ctgcgtcggt
      781 ctggccggtc acgccaagct caaggcgaaa gttgtgaaga agccgcgcgc ggtccggaac
      841 ggcggcaggg ggttcttccg cgtctatgta aggaactacg gaacggccac ggcaaagggc
      901 gtttacctgt ttgcgccctc ggtcaagaag aaggtcggct ggatcgctcc cggcgtcgcc
      961 aggacgatcg gcatccgggc cagggtgcgc ggccggaaag gtcactcccg gaccatcagg
     1021 ttcacggtca agggaaacgg cgtgtccgcc tccgcctttg cgagggtccg ggtgaaatag
     1081 cgccacgcac gcgtatcggg aagggcggcg gcctaggcgg ccgcccttct tcacggtcgg
     1141 gctgccctac agcttcgcca gtttcttttc atcgagctcg acgctgaccg ggcagtggtc
     1201 ggaacccatg acatcaccca ggatctccgc cccggtgatt gcctgcctga ggcgttcgga
     1261 ggcaaggaag taatcgatcc gccagccggc gttccgctcc cgggccttcg acatgtacga
     1321 ccaccaggag taatggccgt tgccttcggc gaagaggcgg aaggtgtcga ggaagccggc
     1381 gtcgagaaag ttctggaacc cctgccgctc ctcgtccgtg aagccgtgct cgccggcatt
     1441 ggccttcggg ttggccaggt catcctcggt gtgagcgacg ttcatgtcgc cgcagaccag
     1501 gaccggcttt ttcgcgtcca gcatcttcat gtgatggagc agggccggat cccactgctt
     1561 ctcgcgcagc ttcagccggg agaggtcccg cttggcgttc ggcacatagg tcgtaaccag
     1621 gaagaagtgg tcgtattcgg cggtgattac ccggccctcg gcccgcgggt cgccgtatga
     1681 atcgttgtgc aggtcgtact cgtccgagat ctcggcggcg aaatcgttca ggaccgcgat
     1741 cggcttctgc cgggtgaaga tcgcggttcc ggagtagccc ttcttcccgg ccgagttcca
     1801 gtactcctcg tattgcggga ggtcgatctc ggcctggccc tgctccgcct tggtctcctg
     1861 catgcagagg atgtcggggt cgtacttctc gacgaaggag ccgaagtcac ccttgcggat
     1921 gacggcccga atgccattga cgttccagga gaagatcttc atcggcccgc cagactaccc
     1981 ggcgggtagg attcggggca tgtggcaggg gagaggctgg cagggcctgg caacggtgat
     2041 cgcgatagcg atttcgattc tggttccggc gacggcaaat gccggcgagg gcggtttcac
     2101 gacggggcgc ctgggcccgt accagatccg gttctccgac tcgccattcg gcttcagcat
     2161 aagcaggggc ggccggtacc tgctggccgg gctgggccac agtttcgagc gaaacggggt
     2221 gcgctacgca accctgggat acaccccgca accggcgaag gagctcgaac cgccggacct
     2281 gtcggcggcg cccgacgaag agccgtccac gcagacctcg tacgtggcga ccagggcaaa
     2341 tggaatgtgg ttcggcccgg atgtttacga ggcgcggcca cggaccgact cgcccggcgg
     2401 ccggcggatg cggctgacga tctggctgag aaaggacgga accctgggaa tccgggcggg
     2461 gatgatggcg ggcaaggcag gctccgtcta cctcgggttc cgctcgccgc aaagcgaggc
     2521 cttccacggt ttcggcggcc gccgggaagg aaccgacctg cgtggttccg acatcaagag
     2581 ctgggtgctc gactaccgct atccggaccc gaccaccgcc tactactctc cggttcccgg
     2641 attcatctca tcgcgtggct atggcctgct gctgaaaggc gaccggatca gccgctggcg
     2701 gatggccagc gaccggccaa cagcctggcg ggtcagcctg cccggcgggt cgatggaaat
     2761 gaccctgacc tcgggcggtc aggcccgggc gatggccgga atctcccggc tgaccggaag
     2821 gcaccgcctg ccgcccgcct gggcgaccgg gaccgttctt tcccgggcga tcgggatcgc
     2881 cgcggactcc ggtggcaaat accagtcgcg ggtcgagtca gatcttgaac atctggccag
     2941 cgcggatttc ccgatctcgg cctacgcctt cgaaggctgg caggcgctgc ccgggagctt
     3001 cgtcaaagcg acgatccgga ccctgaagga agaaggcatc cacccggtgc tttatctgcg
     3061 ttcgttcgtt tcg
//
LOCUS       DAOQMF010000139         3064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17809_length_3064_cov_3.175141, whole genome shotgun sequence.
ACCESSION   DAOQMF010000139 DAOQMF010000000
VERSION     DAOQMF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17809_length_3064_cov_3.175141"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLV77_03310"
     CDS             complement(<1..875)
                     /locus_tag="PLV77_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC14907.1"
                     /translation="MAITAKLRLQNMIDGEFVDPVDGDSEEVINPSTGEVIAESPLSG
                     VEDVNRAVAAAKRAFENGWATTTPRERSDRLLALADAIIENGDELTDLESADAGKPRQ
                     AFIDEELDTTADHIRYFATAARNLEGKAAMEYLEGHTSFIRREPVGPVAQITPWNYPL
                     MMAAWKLGPALATGNTLVLKPAESTPVSTLHTLARICAEVFPPGVTNFIGGHGDPAGS
                     TLVTHPDIKMVSLTGSIGTGKWIAKAAADSLKRVHLELGGKAPVIVFDDADLDAVYET
                     IAGTALFNAGQDCTAA"
     gene            complement(880..2001)
                     /gene="ald"
                     /locus_tag="PLV77_03315"
     CDS             complement(880..2001)
                     /gene="ald"
                     /locus_tag="PLV77_03315"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNC14908.1"
                     /translation="MKVGVIKEIKQDEYRVAITPAGVREFVEHGHEVIIEDGAGEGSA
                     IPNTSYEAQGARILPDASSVFEAAEMILHVKELQASEIEMLKPGHLLFTYLHLAPDPE
                     QTAGLMKSGSTCVAYETVEDAEGRLPLLAPMSEIAGRIATQAGAFMLEKPLGGRGVLL
                     GGVPGVAAANVVVIGGGVVGTNAAFIAMGMAADVFILDRSLDRLRELDLHYAREASTV
                     YSTTLAVEELLPKADLVIGAVLVHGARAPWVIKREQLKLMKPGAVLVDVAIDQGGCFE
                     TSRPTTHRDPTYEVDGIIHYCVANMPGAVPITSTQALTNATLPYALALADNGIQAAVQ
                     RDPGLRPGVNVAAGKVTHEGVAEGTGQQYVPVEEALGWV"
     gene            complement(2073..>3064)
                     /locus_tag="PLV77_03320"
     CDS             complement(2073..>3064)
                     /locus_tag="PLV77_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC14909.1"
                     /translation="LKLARNYHRMRGNGQKAKHIAREVAYHGTTLGALSATGITNLRS
                     QFEPLAPGGCHVPNTNIYRWPEDREPIWAADAIEERILFEGPETVSAVILEPVQNAGG
                     CFVPPDGYFQRVREICDKYDVLLISDEVICAWGRLGHYFGCERFGYQPDIITSAKALT
                     SSYVPMGAMIASDKVAEPFMEGTASFAHGFTFAGHPVGAAVALANIEIFEREDLCGHV
                     LAKEGEFRGMLESLRDIPIVGDVRGAGYFQAIELVKDQETKQSFNDEESEKLLRGFLS
                     GALYERGLICRADDRGDPVIQFSPPLICDTEQFEEIHDVLRPVLTEAASMMRN"
BASE COUNT          470 a          973 c         1074 g          547 t
ORIGIN      
        1 gccgcggtgc agtcctgacc ggcgttgaag agcgcggtgc cggcgatcgt ctcgtagacc
       61 gcgtcgaggt cggcgtcgtc gaagacgatc accggggcct tgccgccgag ttcgaggtga
      121 acccgtttga gcgagtcggc cgcggccttc gcgatccact tgccggtgcc gatcgatccg
      181 gtcagcgaga ccatcttgat gtcgggatgg gtaaccaggg tcgaaccggc cggatcaccg
      241 tggccgccga tgaagttggt cacaccgggc gggaagacct cggcgcagat ccgggccagc
      301 gtatgcaggg tcgagaccgg cgtcgactcg gccggcttca ggaccagtgt gttgccggtc
      361 gcgagcgccg ggccgagctt ccaggcggcc atcatcagcg ggtagttcca gggggtgatc
      421 tgggcgaccg gaccgaccgg ttcgcggcgg atgaacgagg tgtgaccttc caggtactcc
      481 atcgcggcct tgccttcgag gttgcgggcg gcggtcgcga aatagcgaat gtggtcggcg
      541 gtggtgtcga gctcctcgtc gatgaaggcc tggcgcggct tgccggcatc ggccgattcc
      601 agatcggtca gctcgtcgcc gttctcgatg atcgcatcgg caagggcaag caggcggtcg
      661 gagcgctccc gcggggtggt ggtcgcccag ccgttctcga aggcgcgttt ggcggccgcc
      721 accgcccggt ttacgtcctc gaccccggag aggggtgact cggcgatcac ttcaccggtc
      781 gaggggttga ttacctcctc cgagtcgccg tcaaccgggt cgacgaactc gccgtcgatc
      841 atgttctgaa gccggagctt cgcggttatc gccatgggat cagacccagc cgagtgcttc
      901 ctcgaccggt acgtactgct ggccggtgcc ttcggcgacg ccttcgtggg tgaccttgcc
      961 ggccgccacg ttgaccccgg gccggaggcc cggatcccgc tggaccgccg cctggatgcc
     1021 gttgtcggcg agggccaggg cgtagggcag ggtcgcgttg gtgagggcct gggtcgaggt
     1081 gatcggcacc gcgcccggca tgttcgccac gcagtagtgg atgatgccgt cgacctcgta
     1141 ggtcgggtca cggtgggtgg tcggccggga agtctcgaag cagccaccct ggtcgatcgc
     1201 aacgtcaacc agaaccgcgc ccggcttcat cagcttcagc tgttcgcgct tgatcaccca
     1261 gggagcgcgg gcgccgtgga ccagaacagc gccgatcacg aggtcggcct tgggcaggag
     1321 ctcttcaacc gcgagggtgg tcgagtaaac ggtcgaggct tcgcgggcgt agtgcaggtc
     1381 aagttcacgc aggcggtcga gcgaacgatc gaggatgaag acgtccgccg ccatgcccat
     1441 cgcgatgaaa gcggcattgg tgccgaccac gccgccaccg atgaccacga cgttggccgc
     1501 cgcgactccg ggcacgccgc cgagcagcac gccccggccc cccagcggct tctccagcat
     1561 gaaggcgccg gcctgggttg cgatccggcc ggcgatctcg ctcatcggcg ccagcagcgg
     1621 cagccggccc tcggcgtctt cgaccgtctc atacgcgacg caggtcgagc cggacttcat
     1681 caggccggcg gtctgctcgg ggtcgggggc gaggtgcagg taggtgaaga gcagatgccc
     1741 gggcttcagc atctcgatct cggatgcctg cagttccttc acatgcagga tcatctcggc
     1801 cgcctcgaag acggaactgg cgtcgggcag gatccgcgca ccctgcgcct cgtatgaggt
     1861 attcgggatg gcgctgcctt cgccggcgcc gtcctcgatg attacctcgt ggccgtgctc
     1921 gacgaattca cgcaccccgg ccggcgtgat cgccacccgg tactcgtcct gcttgatctc
     1981 cttgatgacg ccgaccttca tctcttacct cgtcctctct ggtggtccgc tggggtgact
     2041 tttccgccgg gcggggtgaa tgggtgaatc gatcagttcc tcatcatgga cgcggcctcg
     2101 gtgaggaccg gcctgaggac atcatgaatt tcctcgaact gctcggtgtc gcagatgagc
     2161 ggcggcgaga actggatcac ggggtctccc cggtcgtcgg cccggcagat caggccgcgt
     2221 tcgtagagcg cccctgaaag gaagccgcgc aacagcttct ctgactcttc gtcgttgaag
     2281 ctctgcttcg tttcctggtc cttgaccagc tcgatcgcct ggaagtaacc ggcgccgcgc
     2341 acgtcaccga cgatcgggat gtcccggagc gattccagca tgccgcggaa ttcgccctcc
     2401 tttgccagca cgtggccgca gaggtcctcg cgttcgaaga tctcgatgtt ggccagggcg
     2461 accgccgcgc cgaccgggtg gccggcgaag gtgaagccgt gcgcgaagga ggcggtgccc
     2521 tccatgaacg gctcggcgac cttgtcggag gcgatcatcg cgcccatcgg cacgtaggag
     2581 gaggtcaggg ccttggccga ggtgatgatg tccggctggt aaccgaaacg ctcgcagccg
     2641 aagtagtggc cgagccggcc ccaggcgcag atcacttcgt cggagatcag cagcacgtcg
     2701 tacttgtcgc agatttcgcg gacccgctgg aagtaaccgt cgggcggcac gaagcagccg
     2761 ccggcgttct ggaccggttc gaggatcacc gcggagacgg tctcggggcc ctcgaagagg
     2821 atccgctcct cgatcgcatc ggctgcccag atcggctccc gatcctcggg ccagcggtag
     2881 atgttcgtgt tcggcacgtg gcatccgccc ggagccagcg gctcgaactg ggaacgaagg
     2941 ttggtgatgc cggtcgccga aagcgcgccg agggtggtgc cgtggtaggc cacctcgcgg
     3001 gcgatgtgct tggccttctg gccgttgcct cgcatgcggt ggtagttgcg ggccagcttg
     3061 agag
//
LOCUS       DAOQMF010000140         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_17862_length_3058_cov_3.454878, whole genome shotgun sequence.
ACCESSION   DAOQMF010000140 DAOQMF010000000
VERSION     DAOQMF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17862_length_3058_cov_3.454878"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLV77_03325"
     CDS             complement(<1..412)
                     /locus_tag="PLV77_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNC14910.1"
                     /translation="MPEAAANITVSLPDGTPLELDPGATGADAALAIGEGLARAALAL
                     KVGDEVRDLSAPVSDGEEISIITSRDPEGLELIRHDAAHVMATAVQELYPGTKVTIGP
                     AIENGFYYDFEFPEGVKITDAELPAIEEAMRRHIK"
     gene            complement(446..1486)
                     /locus_tag="PLV77_03330"
     CDS             complement(446..1486)
                     /locus_tag="PLV77_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC14911.1"
                     /translation="MKSQGVMGVVQKLVGLAIGLLFAMPDSLMRKIFGDPPAAVAALR
                     PDAWAMGRLIGLAEDKRSEWNPEEAREETEFLAGAVSRPVTGVITQDWLLEAGDHSLP
                     ARLFTPGANPLAGPMLVHFHGGGWVQGSINSHHGACAWLAREAGVRVLSVDYRLAPEH
                     PFPAQADDALAAWRSVMSDPARFGADPEKVGVIGDSAGAQMCAVLCLDLKAAGEPQPA
                     CQVLIYPVTDCTGSFPSRESFARGFYLTRARMDWFEDCFVPEGEAENPRVSPLFADDL
                     SGLAPALVSVSLADPLRDEGVAFAGRLIEAGVPVTLDTMPMLHAWFNLTSSRSSRQGH
                     RILAGRITDLLG"
     gene            1550..2962
                     /locus_tag="PLV77_03335"
     CDS             1550..2962
                     /locus_tag="PLV77_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14912.1"
                     /translation="MTGRKCALWLAAAISVLTFATAATANGATRERPCNGAVELCSKT
                     LDQVVLPGTHNSMSNEEYGWILPNQHYAIPTQLEYGVRAFLIDTHYGKPSAGSGPSNV
                     ADWNNGTDGSPKGVAGTYLCHQNCSWGASDLTAELGKVTDFLASHPREVLIFMVEDGI
                     YPQDFATAVNDSALSDYIYTGPTDSYPTLGEMIASNQRVVVFSENHTGNDSDYAEVPW
                     YHRGYNGPMQETPYNFRKDESNVDLTTQQAMAQLTDSANLNSSCRPNRGGTTGQLFLM
                     NHWVNGLIDAGTTGNTFTPDPAVAQVLNQPDVLVARAQACEARRGKLPNIVAVDDFGD
                     GDLTDAVRQLNGVTAKPFFEVAKPKGVTVKSGARASFRLQIRNWGSAESALTRVCASV
                     PGKLAIKPKCQTVFVTQANPGKGTATVKVSTRRIYQGRFKGSKSGPVRFTITGGGDSV
                     TTSSTLKVNPLKPKKPKRRN"
BASE COUNT          581 a         1011 c          988 g          478 t
ORIGIN      
        1 ccttgatgtg gcggcgcatc gcttcttcga tcgccggcag ttcggcgtcg gtgatcttca
       61 cgccctcggg gaactcgaag tcgtagtaga agccgttctc gatcgccgga ccgatcgtca
      121 ccttggtgcc cgggtagagc tcctggaccg cggtcgccat cacgtgcgcg gcatcgtggc
      181 ggatcaactc gagaccctcg gggtcacggc tggtgatgat cgagatctcc tcgccgtcac
      241 tgaccggggc gctgagatca cgaacctcgt ccccgacctt gagtgccagc gcggcccggg
      301 ccaggccttc gccgatcgcc agggccgcgt cggcaccggt cgcgcccggg tcgagctcga
      361 gcggggtgcc gtcaggcagg gatacggtga tgtttgcggc ggcttccggc atcggtcaat
      421 cgtattgaag gcggggacct cgggatcagc cgagcagatc ggtgatccgc ccggccagaa
      481 tccggtggcc ctggcgggag gaacggctgg aggtgaggtt gaaccaggcg tgaagcatcg
      541 gcatcgtgtc gagggtgacc gggaccccgg cctcgatcaa cctcccggcg aaggcgactc
      601 cttcatcccg cagcggatcg gccagcgaca cggaaaccag cgccggggca agaccggaaa
      661 ggtcatccgc aaagagtggc gagacgcgcg ggttttccgc ttctccttcg ggcacgaagc
      721 agtcctcgaa ccagtccatc cgggccctgg tcaggtagaa gcccctggca aacgattccc
      781 gggaggggaa cgacccggtg cagtcggtga ccgggtagat caggacctgg caggcgggct
      841 gcggctcccc cgccgctttc aggtcgaggc agagcaccgc gcacatctgg gcaccggcgc
      901 tgtcgccgat cacccccacc ttctccgggt cggccccgaa gcgggccggg tcgctcatca
      961 cggatcgcca ggcggccagc gcgtcatcgg cctgggccgg gaaagggtgc tccggcgcca
     1021 gcctgtaatc aaccgaaaga acccggaccc cggcttcgcg ggcaagccag gcacaggctc
     1081 cgtgatggga gttgatcgag ccctggaccc aaccgccccc gtgaaagtga accagcatcg
     1141 gcccggccag cggattggcc ccgggagtaa agaggcgggc gggcagtgag tgatccccgg
     1201 cctccagcag ccaatcctgg gtgatcacgc cggtgaccgg acggctgacc gcgccggcca
     1261 ggaactcggt ttcctctcgc gcttcttcgg gattccattc gctgcgcttg tcctcggcca
     1321 ggccgatcag ccttcccatc gcccaggcgt caggcctcag cgcggcgacc gcggccggcg
     1381 ggtcaccgaa gattttccgc atcagtgaat ccggcatcgc gaagagcagg ccgatcgcga
     1441 gtccgaccag cttctgaacg acccccatca ccccttgaga cttcacggag cgcatcgtat
     1501 tggccgcaac tgcgacttca cttcgcggtt agttgagatg atgaattcaa tgaccgggag
     1561 aaagtgtgcg ctgtggctcg ccgcagcgat cagtgtcctg accttcgcta ccgccgccac
     1621 ggcgaacggc gcaacgcgag agcgaccgtg caacggcgcg gtcgagctct gctcgaagac
     1681 gctcgaccag gtggtcctgc cgggcaccca caactcgatg tcaaacgagg aatacggctg
     1741 gatcctgccc aaccagcatt acgcgattcc gacccagctc gagtacggcg tgcgggcgtt
     1801 cctgatcgac acgcattacg gcaagccttc cgcaggatct ggcccctcga atgtcgcgga
     1861 ttggaacaac gggaccgacg gtagcccgaa gggcgtcgcc ggcacctacc tctgccacca
     1921 gaactgcagc tggggagcga gcgacctgac tgccgagctc ggcaaggtca ccgactttct
     1981 ggcttcgcat ccccgcgaag tactgatctt catggtcgag gacgggatct acccccagga
     2041 cttcgccacc gcggtgaatg acagcgccct gagtgactac atctacaccg gtccaaccga
     2101 ctcgtacccc acgctggggg agatgatcgc gagcaaccag agggtggtcg tgttttcgga
     2161 aaaccacacg ggcaatgatt ccgactatgc ggaggttccc tggtaccacc gcggttacaa
     2221 cggaccgatg caggaaaccc cgtacaactt tcgcaaggac gaatccaacg tcgacctgac
     2281 tactcagcag gcgatggcgc agctgaccga ttcggccaac ctgaattcga gttgccggcc
     2341 gaaccggggc ggcaccaccg ggcagctctt cctgatgaac cactgggtca acggactgat
     2401 cgacgccggc acgaccggca ataccttcac tcccgacccc gcggtcgcac aggtcctcaa
     2461 ccagcccgat gtgctcgtcg cccgggcgca ggcatgtgag gcgcgtcggg gcaagctgcc
     2521 gaacatcgtt gccgttgatg acttcggtga tggtgacctg accgacgcgg tccggcagct
     2581 gaacggcgtg accgcgaagc cgttcttcga ggtcgcaaag ccaaagggcg tcaccgtcaa
     2641 gtccggcgcc agggcgagct tcaggctcca gatcaggaac tggggcagcg cggaatcggc
     2701 gttgaccagg gtctgcgcga gcgtccccgg caagctggcg atcaagccga aatgccagac
     2761 ggtcttcgtg acccaggcca accccggcaa gggaacggca accgtgaagg tcagcacccg
     2821 caggatctac cagggccggt tcaagggcag caagtccggc ccggtcaggt tcacgatcac
     2881 cggcggtggt gacagcgtca cgacctcgtc caccctcaag gtgaacccgc tcaagccgaa
     2941 gaagccgaag cgccggaact gagccgggct cccgctgaaa cacgaaaccc ggctggaggg
     3001 ccgggtttcc gagtgggcac aactgggctc gaaccagtga cctccaccgt gtcgaggt
//
LOCUS       DAOQMF010000141         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18063_length_3041_cov_3.531145, whole genome shotgun sequence.
ACCESSION   DAOQMF010000141 DAOQMF010000000
VERSION     DAOQMF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18063_length_3041_cov_3.531145"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLV77_03340"
     CDS             <1..320
                     /locus_tag="PLV77_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC14913.1"
                     /translation="AQAKIGLDPEGRVSFRVGDASKLPFGEDSFDLVTQVNMPVFFGE
                     IARVLRPGGGLIIATSLGEQTPFSTPERLIRRKLASHGFGELNSGAAGEGTWVSAKLE
                     GSG"
     gene            347..1210
                     /locus_tag="PLV77_03345"
     CDS             347..1210
                     /locus_tag="PLV77_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNC14914.1"
                     /translation="MNPSSGGGRSKQLLPDVERRLDALRIPFRVERTRSLEHGVDLAF
                     EAVDNGEIPVVMSGDGLIGAVGGALAGVETKVGLIPAGRGNDLARGLGIPTDPVQAVD
                     CLAAGHTRAIDVGDANGKRFLGIASVGFDSDANRIANEARLLKGQLVYVYAAVRALLA
                     WKPARFSLVENGVQSRLTGYNVVVANNGFYGGGMNVAPDARIDDGQLDVITVGEAGKI
                     RFLLNFPKVFKGTHVEEVESVSFHRTSVIEIGASRPFTIYADGDPLTELPATIRVIPS
                     ALQVIVPEPAS"
     gene            1207..2559
                     /locus_tag="PLV77_03350"
     CDS             1207..2559
                     /locus_tag="PLV77_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNC14915.1"
                     /translation="MIKAKASVARMVGRLSRATGKGGGTTLPGRVLLKLDANAIDKLG
                     ARLTDGTTLISATNGKTTTASMLATVLAGAGRHPVHNRAGSNMTWGVATALTEQRGCE
                     GLFEVDEAWLPEVADRLGPRAILLGNLFRDQLDRYGETEALADAWAGLAARRAGRTSF
                     ILNSDDPLIADISNSSGPDTIYFGIEDEAQALTELQHAFDAKLCRNCGHELTYDRAFV
                     GHLGHYHCEGCGMRRPRPEISATGITLKGMSGLTATINSPEGSVELDLPLPGLYNLYN
                     ALGAIATARQIGVPVSESVRSLAGMKAVFGRVERIRIGEVDLSILLVKNPAGANEVIR
                     TLSLEPAPIDLWIALNDRIADGRDVSWIWDADFEMLADRVGRVTCTGTRASEMALRLK
                     YAGWPTDRTEVIEDPEAAIDRALAQSGEQLFALPTYTALLDLRKLLADRGDTEKYWEG
                     "
     gene            2556..>3041
                     /locus_tag="PLV77_03355"
     CDS             2556..>3041
                     /locus_tag="PLV77_03355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC14916.1"
                     /translation="MILSDENSPEAAIWQLVESCGYTADLPVWSGLAEGASSVLDLGC
                     GIGRVARHLAREGLPVTGVDLDPLLVDDLKRLSAPDPVHALAGDVTGLDGLDLGRERF
                     EVILAPQQLLHILGGRESRQRMIEGVRERLEPDGTAAFAISEWIPEGSREVEVLPDVR
                     EI"
BASE COUNT          524 a         1046 c         1005 g          466 t
ORIGIN      
        1 aggcccaggc caagatcggg ctcgacccgg agggcagggt ctccttccgg gtgggcgacg
       61 cttcgaaact gcccttcggc gaggattcgt tcgacctggt gacccaggtg aacatgccgg
      121 tcttcttcgg cgagattgcc cgggtcctgc gccccggcgg cggcctgatc atcgccacca
      181 gccttggcga acagaccccg ttcagcaccc cggagcggct gattcgcagg aaactggcgt
      241 cccacggctt cggcgagctg aactccggcg cggcgggtga aggaacatgg gtctcggcaa
      301 aactcgaagg atccggctga acttgaaaca ggcgctgaca ttgatcatga acccgagctc
      361 cggcggcgga cgctcgaaac agctcctgcc ggacgtggaa cggcggcttg atgcgctgag
      421 gatccccttc cgggtcgagc gcacccgttc gcttgaacac ggggtcgatc tcgccttcga
      481 ggcggtcgac aatggcgaga tcccggtggt gatgagtggc gacgggctga tcggcgcggt
      541 cgggggtgcc ctggccgggg tcgaaacgaa ggtcgggctg attccggccg gtcgcggcaa
      601 cgacctggcc cgcgggctcg gcatccccac agatccggtt caggccgtcg actgcctggc
      661 cgccggccac acccgcgcga tcgacgtcgg cgatgccaac ggcaagcgct tcctcggcat
      721 cgcctcggtc gggttcgact ccgatgcgaa ccggatcgcc aacgaggcaa ggctcctgaa
      781 gggccagctc gtctatgtct atgcggcggt aagggcgctg ctggcctgga agccggcccg
      841 cttcagcctg gtcgagaacg gcgtccagag ccgcctgacc ggctacaacg tggtcgtcgc
      901 caacaacggc ttttacggcg gcggcatgaa cgtggcccct gacgcccgga tcgacgacgg
      961 ccagctcgat gtgatcacgg tcggcgaagc cggcaagatc cgcttcctgc tcaatttccc
     1021 gaaggtgttc aagggaaccc atgtcgaaga ggtcgagtcg gtctcgttcc accgcaccag
     1081 cgtgatcgag atcggtgcca gccgcccgtt cacgatctac gcggacggtg atccgctgac
     1141 cgaactgccg gcgacgatcc gggtcatccc ctcggcgctt caggtgatcg tgccggagcc
     1201 ggcctcttga tcaaggcaaa agccagcgtc gcccgaatgg tcggccggct gagccgcgcc
     1261 acgggcaagg gcggcggcac caccctgccc ggccgggtcc tgctgaagct ggacgccaac
     1321 gcgatcgaca agctgggcgc ccgcctgacc gacggcacga ccctgatcag cgcgaccaac
     1381 ggcaagacga ccaccgcctc gatgctggcc acggtccttg ccggcgccgg ccggcatccg
     1441 gtccacaacc gggccggctc gaacatgacc tggggtgtgg ccaccgccct gaccgagcag
     1501 cgcggctgcg aaggactctt cgaggttgac gaggcctggc taccggaggt cgccgaccgt
     1561 ctcggacccc gggcgatcct gctcggcaac ctgttccgcg atcaactcga ccgctacggg
     1621 gagaccgagg cgctcgccga cgcctgggcc gggctggccg cccgacgggc cggccggacc
     1681 tcgttcatcc tcaactccga cgacccgttg atcgccgaca tcagcaactc aagcggcccg
     1741 gacacgatct atttcgggat cgaggatgaa gcccaggccc tgaccgagct ccagcacgcc
     1801 tttgacgcca agctctgccg caactgtggc cacgagctga cctatgaccg ggccttcgtc
     1861 ggccatctcg gccactacca ctgcgaaggc tgcggcatga ggcggccccg gccggaaata
     1921 tcggcgaccg gcatcaccct gaagggcatg tcgggcctca ccgccacgat caattccccc
     1981 gaaggcagcg tcgagctcga tctgcccctc cccggcctct acaacctcta taacgcgctc
     2041 ggggcaatcg ccacggcccg ccagatcgga gtgccggtct ccgaatcggt ccggagcctg
     2101 gccggcatga aagcggtctt cggccgggtc gagcggatca ggatcgggga agtcgacctg
     2161 tcgattctgc tggtcaagaa cccggccggc gccaacgagg tgatccggac cctctcactg
     2221 gaacccgcgc cgatcgatct ctggattgcg ctgaacgacc ggatcgccga cggccgcgac
     2281 gtctcctgga tctgggacgc cgacttcgag atgctcgccg accgggtagg cagggtcaca
     2341 tgcaccggaa ccagggcttc ggaaatggcg ctccggctca agtacgccgg ctggcccacc
     2401 gaccgcaccg aggtcattga agatcccgaa gccgcgatcg accgggccct cgcgcaaagc
     2461 ggagagcagc tcttcgccct gccgacctat acggcgctgc tcgatctgcg aaaactgctg
     2521 gccgatcgcg gcgacaccga gaagtactgg gaaggttgat cctcagcgac gagaactcac
     2581 ccgaagctgc gatctggcag ctggtcgagt cctgcggata cacggccgac cttccggtct
     2641 ggagcgggct tgcggaaggt gcatcatccg tccttgacct cggctgcggc atcggcaggg
     2701 tcgcccgcca cctcgcccgg gaagggctgc cggtgaccgg ggtcgatctc gatccgctgc
     2761 tggtcgacga cctcaaacga ctctccgccc cggacccggt ccacgcactg gccggggacg
     2821 tgaccgggct ggacgggctg gacctcggcc gggaacgctt cgaggtcatc cttgcgccgc
     2881 agcaactgct ccacatcctc ggcgggagag agtcccgtca gaggatgatc gagggcgtca
     2941 gggagcgcct cgaaccggac ggcacggccg ccttcgcgat ctccgagtgg attcccgagg
     3001 gctcgcgcga ggtcgaagtg ctgccggacg tccgcgagat c
//
LOCUS       DAOQMF010000142         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18080_length_3039_cov_2.995979, whole genome shotgun sequence.
ACCESSION   DAOQMF010000142 DAOQMF010000000
VERSION     DAOQMF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18080_length_3039_cov_2.995979"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLV77_03360"
     CDS             complement(<1..567)
                     /locus_tag="PLV77_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC14917.1"
                     /translation="MAVVTATSIGIHLGGDPLFSEVSFKLMRGDRMTLSGRNGAGKST
                     LLRILAGEAENDSGSLSFQKGARIALHDQRPPRDQGLSLEEYVYSGRSDVIATERELE
                     RLEAAMSAGEHDDETMRAYAAAQQQLELAGGYRWRDEVLTVLRGIGFGEEEFPRPLSS
                     FSGGELTRASLARALGARADLLLLDEPTN"
     gene            706..2685
                     /locus_tag="PLV77_03365"
     CDS             706..2685
                     /locus_tag="PLV77_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNC14918.1"
                     /translation="MSNGGFDPHPHSGPQYGQTWEARIQSPEAVHGVSPYPPIADYGF
                     LSDGEVSALVAPSGNIEWLCLPRFDSPSIFGSILDRSAGNFRLGPADINVPSHRRYLP
                     GTNVLETSWGTGDGWIIVRDVLLIGPWHHERDRSGTHRRAPTDYDADHVLLRMVRCVH
                     GEVQLTLDCKPVFDYGRVPAQWEYTGDAYHEGLATAPGIDLQLRLNTDMNLGFEGPRT
                     TARTMLKEGDVRFVALSWSEHQAPQHYDEAYDRLVWTAHHWQNWLARGTFPDHPWRGY
                     LERSALTLKGLTYAPTGALVAAATTSLPETPGGERNWDYRFSWIRDSTFTLWGLYTLG
                     FDWEANDFFYFIADVAAGEDDLQIMYGVAGEKELREEILEHLHGYEGASPVRIGNGAY
                     DQRQHDVWGAVLDSFYLHTKSRDGMPEEIWPILKRQVNAALEHWRETDHGIWEVRGKP
                     QHFTSSKVMCWVAVDRGARLARLREEHDLADQWRVAADEIRADICEHAVDERGVFTQH
                     YETDALDASCLLLPLVRFLPPTDERIRNTVLAIADELTEDGLVLRYRVEETDDGLSGE
                     EGSFTICSFWLVSALVEIGELDRARELCERLLGFASSLQLYAEEIDPRSGRHLGNFPQ
                     AFTHLALINAVMHVIRAEQDTTAPRDKTTSPRANP"
     gene            2779..>3039
                     /locus_tag="PLV77_03370"
     CDS             2779..>3039
                     /locus_tag="PLV77_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14919.1"
                     /translation="MKTRLVALLAILVTGLFVAVGPAPAQSAVPGIKQTVAYKRLKSY
                     VGFLQSKRNVPVKTTRRKTYKTTLSNRRNNANTKVLSLYSRTL"
BASE COUNT          533 a         1043 c          941 g          522 t
ORIGIN      
        1 gttggtcggc tcgtccagca gcagcaggtc ggcacgggca ccaagggccc gggcgaggga
       61 ggcgcgggtc agctctccgc cggagaagct ggaaaggggc cggggaaatt cctcttcgcc
      121 gaagccgatt ccccgcagca cggtcagcac ttcgtcgcgc cagcggtagc cgccggccag
      181 ttcgagctgc tgctgggccg cggcgtaggc gcgcatcgtc tcgtcgtcat gctcgccggc
      241 agacatggca gcttcgaggc gttccagttc ccgctcggtc gcgatcacgt cggagcggcc
      301 cgagtagacg tattcctcaa gggaaagccc ctggtcgcgc ggtggacgct ggtcgtgaag
      361 tgcaatccgg gcgcccttct gaaaactgag agatcccgag tcgttttcag cttcgccggc
      421 cagaatccgc agcagggtcg acttcccggc cccgttgcgg cccgaaagcg tcatgcggtc
      481 accgcgcatg agcttgaagg agacctcgga gaagagcgga tcgcccccga gatggatccc
      541 gattgatgtt gcggtgacta ctgccatgga gcgaaaccat aggtagtcgg cgccggcaac
      601 gcccggcctc gtttgaagac ccgcccgccg atcgggcggg tttccgcttg ccacccggtg
      661 aagaccgggt ggcttgctac ggaaaatgtg acagcatttt ccgcaatgag caatggcggc
      721 tttgaccccc acccccactc gggaccccag tacgggcaga cctgggaagc ccggattcag
      781 tctccggagg ccgttcacgg ggtctcgcca tacccgccga tcgccgacta cggcttcctt
      841 tcggacggcg aagtttcggc cctggtcgcg cccagcggca acatcgagtg gctttgcctc
      901 ccccgcttcg actcgcccag catcttcggt tcgattctcg atcgcagcgc cggaaacttc
      961 cggcttggcc cggctgacat caacgtgccg agccaccgcc gctacctgcc cggcaccaat
     1021 gtgctggaaa ccagctgggg cacgggtgac ggctggatca tcgttcgcga cgtcctgctg
     1081 atcgggccct ggcaccacga gcgggaccgc tccggaaccc accgccgcgc cccgaccgac
     1141 tatgacgctg accacgttct gctgagaatg gtgcgctgcg tccacggcga agtccagctg
     1201 accctcgact gcaagccggt cttcgactat ggccgggtcc ccgcccagtg ggagtacacg
     1261 ggtgacgcct accacgaggg tctggccacc gcgccgggga tcgacctcca gctgcgcctc
     1321 aacaccgaca tgaacctcgg cttcgagggt ccgcgcacca ctgcccggac catgctcaag
     1381 gagggtgacg tccggttcgt cgccctgtcc tggagcgaac accaggcccc gcagcattac
     1441 gacgaggcct acgaccggct ggtctggacc gcccatcact ggcagaactg gctggcccgg
     1501 ggcaccttcc ccgaccatcc ctggcgcggc tacctggagc gttcggccct gaccctgaag
     1561 gggctgacct atgccccgac cggagccctg gtcgcggccg ccaccacctc gttgcccgaa
     1621 acccccggcg gtgaacgcaa ctgggattac cggttctcct ggatccgcga ttcaaccttc
     1681 accctctggg gcctttacac gctcggcttc gactgggagg caaacgactt cttctacttc
     1741 atcgccgatg tcgcggcggg cgaggacgac ctccagatca tgtacggggt cgccggtgaa
     1801 aaggagctca gggaggagat cctcgaacac cttcacggtt acgagggcgc ctcaccggtc
     1861 cggatcggaa acggtgccta cgaccagcgc cagcatgatg tctggggcgc ggttctggat
     1921 tccttctatc tgcacacgaa gtcccgcgac gggatgccgg aggagatctg gccgatcctc
     1981 aagcgtcagg tcaacgccgc gctcgagcat tggcgtgaaa ccgatcacgg aatctgggag
     2041 gttcgcggca agccgcagca cttcacttcg tcaaaggtga tgtgctgggt agcggttgac
     2101 cgcggtgccc ggctggcccg gttgcgggag gagcatgacc tcgccgacca gtggcgggtc
     2161 gcggcggatg agatccgcgc tgacatctgc gagcacgcgg tcgacgaacg gggtgtgttc
     2221 acccagcact acgaaaccga tgccctcgat gcttcctgcc tgcttttgcc tctggtccgc
     2281 ttcctgccgc ccaccgacga acggatccgc aacacggtcc tggcgatcgc cgacgaactc
     2341 accgaggacg gactggtgct gcgctaccgg gtggaagaaa ccgatgacgg gctgagcggc
     2401 gaggaaggga gcttcacgat ctgctccttc tggctggtca gcgccctggt cgagatcggc
     2461 gaactcgacc gggcccgcga gctctgcgag cgcctgctgg ggttcgcttc ctcgctgcag
     2521 ctctacgccg aggagatcga cccccgctcg ggccgtcacc tcggcaattt cccgcaggcc
     2581 ttcacccacc tcgccctgat caatgcggtc atgcatgtga tccgggccga gcaggacacc
     2641 accgcccccc gggacaagac cacttccccc cgcgccaacc cctgagtccg gcgaagacgg
     2701 cggccgccgg tgccgatgtt ttcattcctt ttcggatttt cgcaactgat gcctgcggca
     2761 ggtgttagtt tcgacaccat gaaaactcgc ctcgtcgcac tcctcgcaat cctcgtcacc
     2821 ggactgttcg tagcggtcgg acccgcgccg gcccagagcg ccgtccccgg catcaagcag
     2881 accgtcgcct acaaacggct gaagagctat gtcggctttc tccagtcaaa gcgcaacgtc
     2941 ccggtcaaga caactcgacg caagacctac aagaccacgc tttcgaaccg gcgcaacaac
     3001 gcgaacacga aggtgctgag tctctactcg cggaccctg
//
LOCUS       DAOQMF010000143         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18269_length_3023_cov_3.737197, whole genome shotgun sequence.
ACCESSION   DAOQMF010000143 DAOQMF010000000
VERSION     DAOQMF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18269_length_3023_cov_3.737197"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLV77_03375"
     CDS             <1..195
                     /locus_tag="PLV77_03375"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNC14920.1"
                     /translation="SQFTLYGDTRKGNRPSWIEAAGGEEAEPLYDLVCEKLDAKKGIF
                     GADMKVELVNDGPVTLLVET"
     gene            377..814
                     /gene="rimP"
                     /locus_tag="PLV77_03380"
     CDS             377..814
                     /gene="rimP"
                     /locus_tag="PLV77_03380"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNC14921.1"
                     /translation="MNPTPEDIEKRLEAIDPEIEFLALESAGGDALRLFIDRPGGVTL
                     DICQQVTSGLQDVLVDHSLEVSSPGPERPLTKPGHFDRFSGRRAKVVTVEPLDGRRNF
                     TGTIASAGESGVVIESDGQNFDIPYASIERSHLVPEIPQGAAK"
     gene            811..2517
                     /gene="nusA"
                     /locus_tag="PLV77_03385"
     CDS             811..2517
                     /gene="nusA"
                     /locus_tag="PLV77_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNC14922.1"
                     /translation="MTQEIVDAVRALEQEKGIAADTLMDALADALLSAYRKSPGSAKY
                     AEVDLDHETGDFRVFELILPAELEEKLLEEAAAEQERTINPETGEYNEPEEPELDPEM
                     LDPYRDQIERNDVTPDDFGRIAAQTAKQVIYQRIREAERQMMYEEYRDRVGELITGLI
                     QQSDKRYTLVQLRERVEALLPKSEQVYSERYDHGMRVKAVITDVSESTKGPSIVVSRR
                     SPELIKGLFELEVPEIADKLVEIVGVAREPGYRSKIAVVSHADGVDPVGACVGPRGSR
                     VRMVVSELRGEKIDIIPYNEEPARFVAKALSPARVREVLVDDEDMQATVVVPDDQLSL
                     AIGRDGQNARLAARLTGWRVDIKSESEFAREESEHDYEGEEEFDGRCMAVLSSGRRCP
                     NAAVSGSTYCQLPQHQALSRFSTSHVGVLASLSDEDVSVLADPDADEAAVNAIVTAAE
                     ADFVEPTDEDAGEEQVAEIEEAEAETTANEMLVEEAEADLESDDEAAAEVVAEEEAGE
                     ELAAAEEAEAEAELEEAEEAEAEEAEEAEASPEPVDEAEEADQEVAAADEAAEATEGE
                     AE"
     gene            2514..>3023
                     /locus_tag="PLV77_03390"
     CDS             2514..>3023
                     /locus_tag="PLV77_03390"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNC14923.1"
                     /translation="MSKRRVHEIAKEAGVASKDLLAALQAAGIEVKAAASNVEEADAK
                     KVLASLGGKSGEKKPAAKSKAPAKPKAEAKPKEAAKPKAEAKPAAASKAEAKPAAEKP
                     AVKAGSTPKAEAKPKPAARPSQASSKPVRTDAAPSGTPAAPPAASGSPASSGAGGGSA
                     AAGKKRRRVV"
BASE COUNT          633 a          974 c          988 g          428 t
ORIGIN      
        1 agccagttca ccctctacgg cgacacccgc aagggcaacc gcccgagctg gattgaagcc
       61 gcgggcgggg aggaagccga accgctttac gacctcgtct gcgaaaagct cgacgcgaag
      121 aagggcatct tcggtgccga catgaaggtc gaactcgtga acgacggccc ggtcaccctt
      181 ctggtcgaaa cctgacccgg agaacaccgc gagatccgag tttttcgtca ctgtgacgaa
      241 aaagtcggag cccgctgttc ctggttgctg cccgacttga tattcgggct atacttgttg
      301 gtgcttttcc cctctagctg tgtcgaaaag tgggcctcgc ccgctttttt ttggtcctga
      361 acccggaaat acagacttga atcccacccc cgaagacatc gagaaaaggc tcgaagcgat
      421 cgatcccgag atcgagtttc tggcgctgga aagcgccgga ggtgacgccc tgcgcctttt
      481 catcgatcgg ccgggcgggg tgaccctgga catctgccag caggtgacct ccggacttca
      541 ggacgtcctg gtcgaccact cgctcgaggt ttcctcaccg ggccccgaac ggccactgac
      601 caagccaggc catttcgacc gtttctcggg tcgccgggcg aaggtggtca ccgtcgagcc
      661 gctcgacgga cggagaaact tcaccgggac catcgccagt gccggggaaa gcggagtcgt
      721 catcgaatcc gacggccaga atttcgatat tccttacgcg agcattgagc gctcgcacct
      781 cgtgccagaa atcccacaag gagcagccaa gtgacccaag agatcgtcga cgcagttcga
      841 gccctcgaac aggaaaaggg aatcgccgcc gacaccctga tggacgcgct tgccgacgcc
      901 ctgctctccg cctaccgcaa gagcccgggt tcggccaagt acgccgaggt tgatctcgat
      961 cacgagaccg gagacttccg ggtcttcgag ctgatcctgc ctgccgagct cgaagagaag
     1021 ctgctcgaag aagccgccgc cgagcaggag cgcacgatca atccggagac cggcgagtac
     1081 aacgagccgg aagagcccga actcgacccg gagatgctgg atccctaccg cgaccagatc
     1141 gaacgcaatg acgtcacccc ggacgacttc ggccggatcg ccgcccagac cgccaagcag
     1201 gtgatctacc agcggatccg cgaagccgag cgccagatga tgtacgagga gtaccgcgac
     1261 cgggtcggcg agctgatcac cggtctgatc cagcagtcgg acaagcgcta caccctggtc
     1321 cagctgcgcg agcgggtcga ggcgctgctg cccaagtccg agcaggtcta cagcgagcgc
     1381 tacgaccacg gcatgcgggt caaggccgtg atcaccgacg tctccgagtc caccaagggc
     1441 ccgagcatcg tcgtttcgcg ccgcagcccc gagctgatca agggactctt cgaactggaa
     1501 gtgcccgaga tcgccgacaa gctggtcgag atcgtcgggg ttgcccgcga gcccggctac
     1561 cgctcgaaga tcgcggtggt ctcgcatgcc gacggagtcg acccggtcgg cgcctgcgtc
     1621 ggcccccgcg gttctcgcgt gcgcatggtc gtttccgagc tgcgcggcga gaagatcgac
     1681 atcattccct acaacgagga accggcccgc ttcgtcgcca aggcgctttc gccggcccgg
     1741 gtccgcgagg tcctggtcga cgacgaggac atgcaggcga ccgtcgtcgt gccggacgac
     1801 cagctttccc tggcgatcgg ccgcgacggc cagaacgcca ggctagccgc ccgcctgacc
     1861 ggctggcggg ttgacatcaa gtccgaaagc gagttcgccc gggaagagag cgagcacgac
     1921 tacgagggcg aagaggaatt cgacggccgc tgcatggccg tgctttccag cggccgccgt
     1981 tgcccgaacg ccgccgtctc cggctcgacc tactgccagc tgccgcagca tcaggcgctc
     2041 tcgcgcttct cgaccagcca cgtcggtgtg ctggcgagtc tcagtgacga ggacgtcagc
     2101 gtcctcgccg accccgatgc cgacgaagcc gccgtgaatg cgatcgtcac ggccgccgag
     2161 gctgatttcg tcgaaccgac cgacgaggac gccggcgaag agcaggtggc ggagatcgag
     2221 gaagcagaag ccgagacgac cgcgaacgag atgctggtcg aagaggccga ggctgatctc
     2281 gaatccgatg acgaggcggc cgccgaggtc gtagccgagg aggaagccgg cgaggagctg
     2341 gctgccgccg aggaagccga agccgaagca gaactggaag aggctgaaga agccgaagct
     2401 gaagaagctg aagaagccga agcaagtccg gagccggtag acgaggctga agaagccgat
     2461 caggaagtcg ccgcggccga tgaggcagct gaagcgacgg aaggcgaggc cgagtgagca
     2521 agaggcgcgt ccacgaaatt gccaaggaag ccggagtagc cagcaaggat ctgctggcgg
     2581 cacttcaggc tgccggaatt gaagtaaagg cggctgcctc gaacgtggag gaagctgacg
     2641 cgaagaaggt cctggccagc ctcggcggca agtccggcga gaagaagccg gccgcgaagt
     2701 cgaaggctcc cgccaagccg aaggccgagg cgaagccaaa ggaagccgcc aaaccaaaag
     2761 ctgaagcgaa accggccgcc gcgtcgaagg ctgaagccaa accggccgca gagaagcccg
     2821 ccgtcaaagc cgggtcgaca ccgaaggccg aggcgaagcc gaagccggcc gccaggcctt
     2881 cccaggcttc gagcaagccg gttcgcaccg acgctgctcc ctccggaact ccggccgctc
     2941 cgcctgccgc atccggttca ccggcttcgt ccggtgccgg tggaggctcc gctgccgccg
     3001 gcaagaagcg ccgccgcgtg gtg
//
LOCUS       DAOQMF010000144         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18330_length_3018_cov_3.241309, whole genome shotgun sequence.
ACCESSION   DAOQMF010000144 DAOQMF010000000
VERSION     DAOQMF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_3018_cov_3.241309"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLV77_03395"
     CDS             complement(<1..871)
                     /locus_tag="PLV77_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNC14924.1"
                     /translation="MSEEEAAAGEPSPDDAGRIAELREVLNHHIVLYYEKDDPEISDE
                     EYDRLFAELQELEGRNPELITGDSPTQRVGAPASTSFAPVRHAVPMLSLANARTEEEL
                     TAWENRIRNHLLRRDVDPGEITFVTEPKIDGLAISLTYENGRLTKGATRGDGQVGEDV
                     TLNIETIRTVPKQIDDAPPLIEVRGEVYLPIADFAELNQKRVDAGLPAFANPRNSAAG
                     SIRQNDPKAAAERPLAVWSYGIGAMDGIRFASHSEEIEWLRDHGFHVNPDIAVHDSIG
                     EVLDRCHWWLDRRE"
     gene            complement(868..1677)
                     /gene="surE"
                     /locus_tag="PLV77_03400"
     CDS             complement(868..1677)
                     /gene="surE"
                     /locus_tag="PLV77_03400"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC14925.1"
                     /translation="MRILLTNDDGIGAKGLRTLRRELRQIEGVTLDVIAPDSNRSASA
                     RSITTRTPLSVEEIHFDDGDLGHATDGTPVDCVRFAQVGLLGEKPDLIVSGINHGLNL
                     GDDVTYSGTVAAALEGILLGLPAVAVSQQSEKGEMGYMPQRHYEFDTVAKFTAGLVRE
                     MLDHPLPEDTLLNINAPGGEVQGVDVTKLGKRLYRDELKKVERDETNGRTSYRIYGYE
                     PGLDDEPGTDIGAIARGRLTVTPLSLDWTHHDEIEGLRGRDFERLLGAATS"
     gene            complement(1729..2532)
                     /gene="truA"
                     /locus_tag="PLV77_03405"
     CDS             complement(1729..2532)
                     /gene="truA"
                     /locus_tag="PLV77_03405"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNC14926.1"
                     /translation="MNWRLDIEYDGSAFRGWAKQDGGVDTVQARLEEALRIALRTDRP
                     VELTVAGRTDAGVHALGQVASFSFDGEMPPAIVRSLNGLTPRGIAVRAVTPVSGFDAR
                     KDAVSRTYCYRVLTRRPDSPFAVNRAWWVSRPIDRDALDSCAGALIGRHDFTAFTPTE
                     TYHKRFERIIHSAAWTDENGLVDPATGFSAGGDTIQFWVTGDSFMQNMVRILVGTMLE
                     VADGSRSLDNFVQLLEGAERPDAGVTAPPQGLYFVRVDYPDPPPLPVAR"
     gene            complement(2529..>3018)
                     /locus_tag="PLV77_03410"
     CDS             complement(2529..>3018)
                     /locus_tag="PLV77_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14927.1"
                     /translation="PTGPTGSTDGPVINPPNGNNGGNNGNNGNNGNNGNNGNNGNNGN
                     NGNNGKGKTKQAVIKPVKVWKEGGGSVVHPGEAVNMKVSIKNSGDLRATNVYTYMDKK
                     ALSGRPSVDVKRGFNTRVITPGWTVTGTVTIFFGRRAHGKVAITAQAWGNYGKKILYI
                     RR"
BASE COUNT          440 a          976 c         1028 g          574 t
ORIGIN      
        1 cctcgcggcg atccagccac cagtggcagc ggtccagcac ctccccgatc gagtcgtgga
       61 cggcgatgtc ggggttgacg tggaagccgt ggtcccgcaa ccactcgatc tcttcggagt
      121 ggctggcgaa cctgatcccg tccatcgccc cgatcccgta tgaccagacc gcgagcggac
      181 gctcggccgc ggccttcggg tcgttctgcc ggatcgatcc ggccgcggag ttgcgcgggt
      241 tggcgaaggc gggcaggccg gcgtcaaccc gcttctggtt gagctcggcg aaatcggcga
      301 tcggcaggta gacctcgccc cgaacctcga tcaggggcgg ggcgtcgtca atctgcttcg
      361 gcaccgtcct gatcgtctcg atgttgaggg tgacgtcctc cccgacctga ccgtcaccgc
      421 gggtcgcgcc cttggtcagt ctcccgttct cgtaggtgag cgagatcgcc aggccgtcga
      481 tcttcggttc ggtgacgaag gtgatctcgc ccgggtccac gtcacggcgc agcaggtggt
      541 tccggatcct gttctcccag gcggtcagct cttcttcggt tctggcgttg gccagagaaa
      601 gcatcgggac cgcatgcctg accggggcga agctggtcga agccggggcg ccgacccgct
      661 gcgtcggcga gtcaccggtg atcagttccg ggttccggcc ctcgagttcc tggagttcgg
      721 cgaaaagccg gtcgtattcc tcgtcggaga tctccgggtc gtccttctcg taatagagga
      781 cgatgtgatg gttcaggact tcacgcagtt cggcgatccg accggcgtcg tcgggtgacg
      841 gttccccggc cgctgcctct tcctcgctca agaggtggcc gctcccagca gccgttcgaa
      901 atcgcggccg cgcaggcctt cgatctcgtc gtgatgggtc cagtccaggc tgagcggggt
      961 gaccgtgagc cggccgcggg caatcgcgcc gatgtcggtg ccgggctcgt cgtcgaggcc
     1021 cggctcgtag ccgtagatcc ggtaggaggt acggccgttg gtctcgtccc gctccacctt
     1081 cttcagctcg tcgcggtaga gccgcttgcc cagtttggtc acgtcgacgc cctgcacctc
     1141 gcccccgggc gcgttgatgt tgagcagggt gtcttcgggc agcggatggt cgagcatctc
     1201 gcggaccagc ccggcagtga acttcgcgac cgtgtcgaac tcgtaatggc gctgcggcat
     1261 gtagcccatc tcccccttct ccgactgctg cgaaacagcg accgccggca gaccgagcag
     1321 gatcccctcc agggcggcag cgacggtgcc cgaataggtg acgtcatcac cgaggttgag
     1381 cccgtgattg atgccggaga cgatcaggtc gggcttttcg ccgagcaacc cgacctgggc
     1441 gaagcgcacg cagtcgaccg gggtgccgtc agtcgcgtgg ccgaggtcgc cgtcgtcgaa
     1501 gtggatttcc tcgaccgaaa gcggggtgcg cgtagtgatc gagcgggccg aggcgctgcg
     1561 gttcgagtcg ggagcgatca cgtccagggt gacgccttcg atctgccgca gttcgcggcg
     1621 cagcgtccgc aggcccttgg caccgatccc gtcgtcatta gtcagaagaa tcctcacagg
     1681 gtgaggatat gaaacccggg aggcggccag ccgacggcgc gcctcgcgtc accgggcaac
     1741 gggcagcggc ggcgggtccg ggtaatcgac tctcacgaag tagaggccct gcggcggggc
     1801 ggtgaccccg gcatccggtc gttcggcgcc ctccagcagc tgaacgaagt tgtccaggct
     1861 gcggctcccg tcggccacct ccagcatggt gccgaccagg atccggacca tgttctgcat
     1921 gaaggagtcg ccggtgaccc agaactggat tgtgtctccg cccgcggaga agccggtggc
     1981 cgggtcaacc agtccgttct cgtccgtcca tgctgcggaa tggatgatcc gctcgaaccg
     2041 cttgtggtag gtctcggtcg gagtgaaggc ggtgaagtcg tgccggccga tcagcgcccc
     2101 ggcgcaggag tcgagcgcgt cacggtcgat cggccgtgag acccaccagg cccggttcac
     2161 ggcgaacggg ctgtcgggac gccgggtcag gacgcggtaa caataggtcc gcgagacggc
     2221 gtccttgcgc gcatcgaagc cggagaccgg ggtcaccgcc cggaccgcga tcccccgcgg
     2281 ggtgagtccg ttcaggctgc gcacgatcgc cggcggcatc tcgccgtcaa agctgaaact
     2341 ggcgacctga ccgagggcgt ggaccccggc gtcggttcgt ccggcgacgg tcagctcaac
     2401 cggccggtcg gttcgcaggg ctatccgcag cgcttcttca agccgcgcct gcacggtgtc
     2461 gacgccacca tcctgcttcg cccagccccg gaaggcggag ccgtcgtact cgatgtcgag
     2521 acgccagttc agcggcggat gtagaggatc ttcttgccgt agttgcccca ggcctgggcg
     2581 gtgatcgcca ccttgccgtg agcgcgcctg ccgaagaaga tcgtgacggt tccggtgacg
     2641 gtccagcccg gagtgatgac cctggtgttg aaacctcgct tgacgtcgac gctgggccgg
     2701 ccggagaggg ccttcttgtc catgtaggtg tagacgttgg tggccctcag gtcaccgctg
     2761 ttcttgatcg aaaccttcat gttgacggcc tcgccgggat ggacgacgct gccgccgcct
     2821 tccttccaga ccttgaccgg cttgatcacg gcctgcttgg tcttgccctt gccgttgttg
     2881 ccgttattgc cgttgttccc gttgttgccg ttattgccgt tgttcccgtt attaccgttg
     2941 ttgccgttat tgccaccgtt attgccgttc ggcgggttga tcacgggacc atcggtggaa
     3001 ccggtcggcc cggtcggc
//
LOCUS       DAOQMF010000145         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18352_length_3016_cov_3.834515, whole genome shotgun sequence.
ACCESSION   DAOQMF010000145 DAOQMF010000000
VERSION     DAOQMF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18352_length_3016_cov_3.834515"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLV77_03415"
     CDS             complement(<1..437)
                     /locus_tag="PLV77_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC14928.1"
                     /translation="MNEQVRADEASFVMQTYKRAPVEFVRGEGALLFDREGRQYIDFL
                     GGISVASVGHCHPAVVEAIRQQAGELLHVSNLFYTAPMVELAKRLSESSLGGKVFFCN
                     SGTEANECAIKIARKHARSRGVESPEIVSFESDFHGRSYGALSA"
     gene            complement(434..1306)
                     /gene="argB"
                     /locus_tag="PLV77_03420"
     CDS             complement(434..1306)
                     /gene="argB"
                     /locus_tag="PLV77_03420"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNC14929.1"
                     /translation="MDPKDIANPIIDDDSVNVLLEALPYIREFHGKTVVIKYGGAAMR
                     EESLRQAFADDVVLLKYVGLNPVIIHGGGPEITKYMNKMGIEVRFQHGLRVSDAETVE
                     IAKMVLLGKLNADLVARLGRAGSPAVGLSGEDGGLFDVAPVANAEEVGFVGDIERVDI
                     DVLNHISEDYIPVIASVGSDRDGNSYNINADEAAGKVAAALGAFKVVFLTDIVGWLSD
                     PEDESSRISETTVAEVEDALESISGGMRPKLTACVDAIRGGAQNAYIIDGRRPHSLLL
                     ELFTDAGIGTMVTP"
     gene            complement(1330..2517)
                     /gene="argJ"
                     /locus_tag="PLV77_03425"
     CDS             complement(1330..2517)
                     /gene="argJ"
                     /locus_tag="PLV77_03425"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNC14930.1"
                     /translation="MTGYPSLSVPPEGDFFRSRWVSPPAGIEFNEDPVLPDGFRAGGV
                     ACGLKGGGVTDLGAVVCDSGEVSSAILLTANAAAAAPIRVCRESLWQGEVRAAIVNSG
                     NANAATGEQGILDALSMRNSLATELGLAPQTVGVAETGVIGVPMDMEAVISGIGGLAE
                     SLSAEGAVSFSEAIMTTDKWPKRVSLSVDGITLSAQAKGAGMIEPGFATMLCFVQTDA
                     VVPEPEAALRQAVSGSFERITVDGQMSTNDTVLLQASGSSGRELPAGLLDAVLLQLAL
                     EIVSDGEGASRVGRVEVTGAADQAEAELVARKIANSPLVKTALLGRDPNWGRIAQAAG
                     QALAGHDIGELGPEVIEAGDIAGGGREVELALRLDRGDGTAHVFTSDLTHEYIVINAE
                     YTT"
     gene            complement(2514..>3016)
                     /locus_tag="PLV77_03430"
     CDS             complement(2514..>3016)
                     /locus_tag="PLV77_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HNC14931.1"
                     /translation="KSGVSGAGRGGGESTAFVTVTENVNAYKPAGHRHRPEIVEKLGL
                     IAAAGGNPEAAPDRLTFVPHLVPVDQGELVSCYVTPRRPVAQAELDALYTSAYADEPF
                     IRLRDVPPGMRDVRETNFCDIFPLVTDDRILVESAIDNLWKGASSQAIQNLNLMLGLD
                     ETEGIG"
BASE COUNT          477 a         1020 c          993 g          526 t
ORIGIN      
        1 gctgaaaggg caccgtaact gcggccatgg aagtcgcttt caaacgagac gatttccggg
       61 ctttcgaccc cgcggctccg ggcgtgcttc cgggcgatct tgatcgcgca ttcgttggct
      121 tcggtgccgg agttgcagaa gaagaccttg ccaccaagcg agctctccga gagccgtttg
      181 gccagctcga ccatcggggc ggtgtagaag aggttcgaga cgtgaaggag ttctccggcc
      241 tgctgccgga tcgcctccac gaccgccggg tggcagtggc cgaccgaggc gaccgagatg
      301 cccccgagga agtcgatgta ctgacggcct tcccggtcga agagcagcgc gccttcgccc
      361 cggacgaact cgaccggggc ccgtttgtag gtctgcatta caaaactggc ctcgtcggcc
      421 cggacctgct cgttcacggg gtcaccatgg tgccgatgcc ggcgtcggtg aagagttcga
      481 gcagcagcga atggggacgg cggccgtcga tgatgtaggc gttctgggcg ccgccgcgga
      541 tcgcgtccac gcaggcggtc agcttgggtc gcatgccgcc ggagatcgac tccagggcat
      601 cctcgacttc ggccacggtg gtctcggaga tccgcgaact ctcatcctcc ggatcgctca
      661 gccagccgac gatgtcggtc aggaaaacga ccttgaaggc gccgagcgcc gccgccacct
      721 tgccggcggc ctcatcggca ttgatgttgt acgagttgcc atcccggtcg gatccgaccg
      781 aggcgatcac cgggatgtaa tcctcgctga tgtggttgag cacgtcgatg tcaactcgct
      841 cgatgtcacc gacgaatccg acttcctcgg cgttggctac cggcgcgaca tcgaagaggc
      901 cgccgtcttc accggaaagt cccacggcag gcgagccggc ccggccgagt cgggccacga
      961 gatcggcgtt gagcttgccg aggagcacca tcttcgcgat ctcgaccgtt tccgcgtcag
     1021 agaccctaag gccgtgctgg aagcggacct cgatgcccat cttgttcatg tatttggtga
     1081 tctcgggccc gcctccgtgg atgatcacgg gattgaggcc gacgtacttg agcaggacca
     1141 cgtcatcggc aaacgcctgt cgcagcgact cttcgcgcat cgcggccccg ccgtacttga
     1201 tcaccacggt cttgccgtgg aactcgcgaa tgtaaggcag ggcctcgagc agcacgttca
     1261 cggagtcgtc atcgatgatc gggtttgcga tgtccttcgg atccattgct ctttccttgt
     1321 ctgcctgtgc tacgtcgtgt attccgcgtt gatcacgatg tactcgtggg tcaggtcgct
     1381 ggtgaaaacg tgggccgtac cgtcgccgcg atccagccgc agggccagtt ccacttcgcg
     1441 gccgcctccg gcgatgtccc cggcctcgat cacctcgggg ccaagctcgc cgatgtcatg
     1501 cccggccagg gcctggccgg cggcctgggc gatccgcccc cagttcggat cgcgaccgag
     1561 cagggcggtc ttgaccagag gcgagttggc gatcttgcgc gcgaccagct cggcctcggc
     1621 ctgatcggcc gccccggtca cctcgacccg accgacccgg ctggcgcctt cgccatcgga
     1681 gacgatttcc agcgcgagct gaagcagcac cgcgtcgagc agacccgctg gaagctcccg
     1741 gccggagctg ccgctggcct gaagcagcac cgtgtcattc gtgctcatct ggccatcgac
     1801 cgtgatccgt tcgaacgagc cgctcacggc ctgcctgagc gccgcctccg gttcgggaac
     1861 cacggcgtcg gtctggacga agcagagcat ggtggcgaag ccgggttcga tcatcccggc
     1921 gcccttcgcc tgggcggaga gggtgattcc gtcaacgctc agcgaaaccc gcttcggcca
     1981 cttgtcggtg gtcatgatcg cctccgagaa actgaccgcc ccttcggccg agagcgattc
     2041 ggcgaggccg ccgatgccgg agatcaccgc ttccatgtcc atcggcacgc cgatcacccc
     2101 ggtctcggcg acccccacgg tctgcggggc cagccccagc tcggtggcca gcgagttgcg
     2161 catcgaaagc gcgtcgagaa tcccctgctc gccggtggcg gcgttggcgt tgccggaatt
     2221 gacgatcgcc gcccggacct cgccttgcca gaggctttcc cggcagaccc ggatcggcgc
     2281 cgcggccgcc gcattggcgg tcagcaggat cgccgagctg acctccccgg agtcgcagac
     2341 gacggctccc aggtcggtca ccccgccgcc cttcaggccg caggcgaccc ctccagcccg
     2401 gaagccgtca ggcagcaccg ggtcttcgtt gaactcgatc ccggccggcg ggctcaccca
     2461 gcgggagcgg aagaaatccc cctccggcgg aacgctgagg ctcgggtagc cggtcatccg
     2521 atcccctcgg tttcgtcgag accgagcatc aggttcaggt tctggatcgc ctgcgaggag
     2581 gcgcccttcc agaggttgtc gatcgccgat tcgaccagga tccggtcatc ggtgaccagc
     2641 gggaagatgt cgcagaaatt ggtctcgcgc acgtcccgca tgcccggcgg cacgtcccgc
     2701 aaacggatga acggctcatc ggcgtaagcc gaggtgtaga gcgcgtcgag ttcggcctgg
     2761 gcaaccgggc ggcgcggggt cacgtaacag ctgaccagtt ctccctggtc gaccgggacc
     2821 aggtgcggca cgaaggtcag gcggtcgggc gccgcttcag ggttgccgcc ggcagccgcg
     2881 atcaggccga gcttctcgac gatctcgggc cggtgccggt ggccggccgg cttgtaggcg
     2941 ttgacgttct cggtcaccgt cacgaaggcg gtgctctctc ccccgccccg gccggccccg
     3001 gaaacgccgg acttgg
//
LOCUS       DAOQMF010000146         2994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18587_length_2994_cov_4.284791, whole genome shotgun sequence.
ACCESSION   DAOQMF010000146 DAOQMF010000000
VERSION     DAOQMF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18587_length_2994_cov_4.284791"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLV77_03435"
     CDS             <1..377
                     /locus_tag="PLV77_03435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC14932.1"
                     /translation="LGLENARAIKARSEDFAKGEGREAFDLVTARAVAPLAALAELAS
                     PLLREGGHLLAWKGEPEPDSEQVIAANRDRLAMEIEGVREVMPYEGSRDRRLYLLVKA
                     GPTPDGLPRRPGMARKRPLGGG"
     gene            442..1242
                     /locus_tag="PLV77_03440"
     CDS             442..1242
                     /locus_tag="PLV77_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC14933.1"
                     /translation="MRIPSCPDLFGDRGIQLIYSVANQKGGVGKTTTAVNLGAAYAQL
                     GKRVLVVDLDQQCNATVAFGLEKQDSPTTYEVLSGEVTLSEAAKPAGPENVWMVPASR
                     DLAGAVVELPRLERPNHRLQEQVGSVDEDFDVVFIDCPPALGPITVNSLVASDRVIVP
                     VQAEYLALEGLVEFLDTLSTVRREMNPTLELAGVLVTMHDERTRLAQDVERELRDHFS
                     ETVFRTVIPRTVRVAEAPSYGIPVTDYSPSSRGSEAYLALAEEVMARG"
     gene            1235..2083
                     /locus_tag="PLV77_03445"
     CDS             1235..2083
                     /locus_tag="PLV77_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNC14934.1"
                     /translation="MAERRGMGRGLGAILPESATGPDMRELPVKSIDRNPEQPRTNFS
                     EEALEALAESIRASGVVQPLIVRPAGRGKYEIIAGERRWRAAQMAGVEKVPVVIRDSD
                     EADRLEVALIENMVRENLNPVEEAKACAALVEDLGLSKEELGKRVGRSRPQISNLIRL
                     LDLPDDVLEMLEGGELSEGHGRAILQVPDHADRRKLAKQARKEGWSVRQTEAGARALA
                     AAPKKGGRKQGLSAEESEAVAEATERLETKLDRPVKIRPKGKGLVVEIELEDLAQAAE
                     IARRLK"
     gene            2171..2244
                     /locus_tag="PLV77_03450"
     tRNA            2171..2244
                     /locus_tag="PLV77_03450"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2205..2207,aa:Ile,seq:gat)
     gene            2403..2891
                     /locus_tag="PLV77_03455"
     CDS             2403..2891
                     /locus_tag="PLV77_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNC14935.1"
                     /translation="MREVQIYGVSFDMVGKQPIVLLKTIDDNRYLPIWIGHPEAAAIL
                     MKLQGADTPRPMTHDLIVDMLDQVETKVERISVNELRDNTFFATITLSVSGTEIEIDS
                     RPSDALALAVRLQAPIFVAEEVIEESSIEFDQGIEDNEQVVEKFKDFLDDVSPEDFLQ
                     DS"
BASE COUNT          569 a          956 c          972 g          497 t
ORIGIN      
        1 ctctgggact ggaaaatgcc cgggcgatca aggcccggtc ggaagatttc gcgaagggcg
       61 aggggcgcga ggcgttcgat ctcgtcacgg cgcgggcggt tgctccgctt gccgccctgg
      121 ccgaactcgc ctctcccctg cttcgcgagg gagggcacct tctcgcctgg aagggtgagc
      181 cggaacccga ctcggaacag gtcatcgccg ccaaccggga tcgcctcgcg atggagatcg
      241 agggggtccg ggaagtaatg ccctacgagg gatcacgcga ccggcgcctc tacctgctgg
      301 tcaaggcagg gccaaccccg gacggcctcc ccaggcgtcc gggcatggcc agaaagcgac
      361 cgttgggcgg cggttgacca gtgaccggtc gcgtccggca aacaaataag ctggcctcta
      421 catgaggcat tcttcgtcca cgtgcggatt ccttcctgtc cggacctctt cggtgaccgg
      481 ggcattcagt tgatctattc ggtcgccaac cagaagggag gagtcggcaa gaccaccact
      541 gccgtcaacc tcggggcggc ctacgctcag ctgggcaagc gggtgctggt cgtcgatctc
      601 gaccagcaat gcaacgcgac ggtcgccttc ggcctcgaga agcaggattc gccgactacc
      661 tacgaggtgc tgagcggcga ggtcaccctc tccgaggcgg ccaagccggc cggtcccgag
      721 aacgtctgga tggtcccggc cagccgtgac ctggccggag cggtggttga actgccccgg
      781 ctcgaacgac cgaatcaccg gctgcaggag caggtcggat cggtcgacga ggatttcgac
      841 gtggtcttca tcgactgccc gcccgcgctc ggaccgatca ccgtcaactc gctggtcgcc
      901 tccgaccggg tgatcgtgcc ggtccaggcc gagtacctgg cgctcgaagg cctggtcgag
      961 tttctcgaca ccctttcgac cgtgcggcgg gagatgaacc cgaccctgga gctggctggc
     1021 gtcctcgtca ccatgcatga tgagcggacc agacttgccc aggatgtcga gcgtgagctt
     1081 cgcgatcact tctccgagac tgtgttccgg acggtgatac cgaggaccgt gcgcgtcgcg
     1141 gaggccccga gttacggcat cccggtgacc gactattccc cttcatcaag gggttccgag
     1201 gcctatcttg ccctggccga agaggtgatg gcacgtggct gagcggcgcg gaatggggcg
     1261 gggcctcggg gcgatcctgc cggaaagcgc caccgggcct gacatgcgcg agctgccggt
     1321 aaagtcgatc gaccgcaatc cggagcagcc ccggaccaac ttttccgagg aggctctcga
     1381 ggccctcgcc gaatcgatcc gggccagtgg agtggtccaa ccactcatcg tgcggccggc
     1441 tgggcgcgga aagtacgaga tcatcgccgg cgagcgacgc tggcgggccg cacagatggc
     1501 cggggtcgaa aaggtcccgg tggtcatccg tgattccgat gaagccgacc ggctcgaggt
     1561 cgccctgatc gagaacatgg tccgggaaaa cctgaatccg gtggaagagg caaaggcctg
     1621 cgccgccctg gtcgaggatc tcggtctcag caaggaagaa ctcggcaagc gggttggccg
     1681 cagccgcccc cagatctcca acctgatccg gctgctcgac ctgcccgacg acgtgctcga
     1741 aatgctcgaa gggggcgaac tttccgaagg ccatggacgc gcgatcctcc aggttcccga
     1801 ccacgccgat cgccgcaagc tggccaaaca ggccaggaag gaaggctggt cggttcgtca
     1861 gaccgaggcc ggggctcgcg ctctcgctgc ggcgccgaag aagggcggcc gcaagcaggg
     1921 tctgtcggcc gaggagagtg aagccgtcgc cgaagcaacg gagcggctgg aaaccaaact
     1981 tgatcgcccg gtgaaaatca gaccgaaggg gaaggggctg gtggtcgaaa tcgaactcga
     2041 ggacctcgcc caggcggctg agatcgccag gcgtctcaag taagtcgagg ctccgggctc
     2101 gtcccggcgc ggtgtctcct caccacacga cacccgggcc cgacccccat tagaatcctc
     2161 ggtccctcga gggcgattag ctcagttggt tagagcgcat ctctgataag gatgaggtcc
     2221 ctggttcgaa tccaggatcg cccataggct tgaccgcagt ccggcgggtc cgcgcccgcc
     2281 ctccaatctc aaccggcctt cccgctcaca caaaatgacg gatgggagga cctccgttcg
     2341 agccgacacg gcggaacggc tgatgaggtg accagggctc gtcctaccta taatcgggat
     2401 ccatgcgtga ggttcagatc tatggcgtca gcttcgacat ggtcggcaag cagccgatcg
     2461 ttttgctgaa gacgatcgac gacaatcgct acctgccgat ctggatcggg caccctgaag
     2521 cggccgcgat cctgatgaag ctccagggtg ccgacacgcc gcggccgatg acccacgacc
     2581 tgatcgtgga catgctcgac caggttgaaa ccaaggtgga acggatctcg gtcaacgagc
     2641 ttcgcgacaa caccttcttc gccacgatca cgctctcggt ctcaggcacc gagattgaaa
     2701 tcgattcacg cccctccgac gcgctggccc tggcggtcag gctccaggca ccgatctttg
     2761 tggccgagga agtgatcgag gaatcctcga tcgagttcga ccagggcatc gaagacaatg
     2821 agcaggtggt cgagaagttc aaggacttcc tcgacgacgt ctcccccgaa gacttcctcc
     2881 aggactctta actgcagtcg gcgagcttcg ccccgcctcc aggttcaacc cgcccctccc
     2941 actcgcaacc acaatggttg ggaggggctc tactgggtgg ccaagtcgag gatt
//
LOCUS       DAOQMF010000147         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18612_length_2992_cov_3.564522, whole genome shotgun sequence.
ACCESSION   DAOQMF010000147 DAOQMF010000000
VERSION     DAOQMF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18612_length_2992_cov_3.564522"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PLV77_03460"
     CDS             complement(<1..820)
                     /locus_tag="PLV77_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HNC14936.1"
                     /translation="MFVHKAAVVGGGTMGGQIAQAIAAADIPVVVKDVDQKFVDHAIE
                     EVRNVTAGQLASKVKKEKITQEQADARLEEVLANVTGTVTYDEFGDVDFVIEAVPEKM
                     AIKQAVFAELDAVTPGHAILSSNTSALSISEIGEATLRPDKVVGFHFFYPASVMPLVE
                     VVIGDDTSSETAQTSSNFAQAIGKSPITCGEVPGFVVNRILNSAVGEIWRAQEERGLS
                     IAKIDEGVGAANVSPMGPFILIDVLGLDTVYHVAEHLNDSYGERFYVHQGMKQLV"
     gene            1123..2424
                     /locus_tag="PLV77_03465"
     CDS             1123..2424
                     /locus_tag="PLV77_03465"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC14937.1"
                     /translation="MSQQSFADLGVSNAVVNSLRDRGIEAPFPIQRQVIEDILEGDDV
                     LVQSPTGSGKTLAFGVPLVDLVESTDPRPTALILAPTRELASQIVDEMEAICHARALR
                     IAAVYGGVGLQQQAKRAAKSHIIVACPGRLEDQLQRGAFDLENVRYLVLDEADRMLDM
                     GFKPAVDRIVRQIDDSRQTLFFSATLEGAAGKLAAAYTIDPKTHVNRTPKEKQGTIEH
                     RFIHVSHEAKVETLVGELESPDRGRTLVFVRTKHGADRLVKKLSKRSRQVRAVAMHGN
                     KSQNQRQRALADFEDGRVDTLVATDVAARGIDVADVTHVINFDIPEDQDTFVHRVGRT
                     GRAGADGVGISFFMSDQAKEMGSIAKSLGLHQEFEASGAGFHAESHTRQKRSGGNGSR
                     NFRLSPRRKRVGSCRPSPGGVTVGGRSSAGRSLVVRSVAGG"
     gene            complement(2679..2762)
                     /locus_tag="PLV77_03470"
     tRNA            complement(2679..2762)
                     /locus_tag="PLV77_03470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2727..2729),aa:Leu,seq:caa)
BASE COUNT          532 a          974 c          928 g          558 t
ORIGIN      
        1 cgaccagctg cttcatgccc tggtggacgt agaaacgctc gccgtaggag tcgttcaggt
       61 gctcggcgac gtggtagacg gtgtccagac cgaggacgtc gatcaggatg aacgggccca
      121 tcggggaaac gttcgcggcg ccgacgcctt cgtcgatctt ggcgatcgag aggccgcgct
      181 cttcctgggc gcgccagatc tcgccgaccg cggagttgag gatgcggttg acgacgaatc
      241 cggggacctc gccgcaggtg atcggggact tgccgattgc ctgggcgaaa ttcgacgaag
      301 tctgagcggt ctcggacgag gtgtcgtctc cgatcacgac ctcgaccagc ggcatgaccg
      361 aggccgggta gaagaagtgg aagccgacga ccttgtcggg acgcagcgtg gcctcgccga
      421 tctcggagat cgagagggcc gaggtgttgg aagaaaggat cgcgtggccg ggggtgaccg
      481 cgtcaagctc ggcaaagacg gcctgcttga tcgccatctt ctccggcacg gcctcgatca
      541 cgaagtcgac gtcaccgaac tcgtcgtagg tgacggtgcc ggtgacgttg gccaggacct
      601 cttcgaggcg ggcatcggcc tgctcctggg tgatcttttc cttcttgacc ttgctggcaa
      661 gctggccagc ggtgacgttc ctgacttcct cgatcgcgtg atcgacgaac ttctggtcga
      721 catccttgac tacgaccggg atgtcagctg cggcgatcgc ctgggcgatc tgaccgccca
      781 tggtgccacc gccgacgacg gctgccttgt gtacgaacat gaggtttctt ggtcctccgg
      841 gggcttggtg agttgagagc cttgcgggtg tctgacccga ctgcgcgcga gaagttgact
      901 cgcgagtcaa taaggctatc gggactgtgg tcgacacgcc gccagcaccg cgtaaatccg
      961 tcctttgcag aggtttcctt tatccggcct gttatcgttt gccgcggtcg cagtccctga
     1021 cggacgcgat cgaggttctg tttccttcct gtttcgcgcg gcttaccggc gtcggggttg
     1081 ttaccggatc tcccccttac ccctcacagg aagttgaaaa gcatgtccca gcagtccttt
     1141 gccgatctcg gcgtgtcaaa tgccgtggtc aattcgctcc gcgatcgcgg aatcgaagcc
     1201 ccgttcccca tccagcgtca ggtcatcgag gacatcctcg aaggcgatga cgtgctggtc
     1261 cagtccccca ccggttccgg caagacgctt gccttcggtg tgccgctggt cgacctggtc
     1321 gaatccaccg accctcgccc gaccgcgctg atcctcgccc cgacccgcga gctggccagc
     1381 cagatcgttg atgaaatgga agcgatctgc cacgcccgcg cactccggat cgccgctgtc
     1441 tacggcgggg tcggcctgca gcagcaggcc aagcgggccg cgaagtccca catcatcgtc
     1501 gcctgcccgg gccggctgga agaccagctc caacgcggcg cattcgacct tgaaaacgtg
     1561 cgctacctgg tcctcgacga agccgaccgc atgctcgaca tgggcttcaa gccggcggtc
     1621 gaccggatcg tccgccagat cgacgacagc cgccagaccc tcttcttctc ggccacgctc
     1681 gaaggtgctg ccggcaagct ggccgcggcc tacacgatcg accccaagac ccatgtcaac
     1741 cggacgccga aggagaagca gggaacgatc gagcaccgtt tcatccacgt ttcgcatgaa
     1801 gccaaggtag aaaccctggt gggcgaactc gaaagcccgg accgtggccg taccctggtc
     1861 ttcgtccgga ccaagcatgg tgccgaccgg ctggtcaaga agctcagcaa gcgcagccgg
     1921 caggttcgcg cggtcgccat gcacggcaac aagtcgcaga accagcggca gcgcgccttg
     1981 gccgacttcg aggatggccg ggttgacacc ctggtcgcca ccgacgtcgc cgcccgtggc
     2041 atcgacgtcg ccgacgtgac ccacgtaatc aacttcgaca tccccgagga tcaggacacc
     2101 tttgtccacc gggtcggccg caccggccgg gccggcgccg acggggtcgg gatcagcttc
     2161 ttcatgtccg accaggcgaa ggagatggga agcatcgcga agtcgctcgg gctccatcag
     2221 gaattcgaag ccagcggcgc cgggttccac gccgaaagcc acacccgcca gaagcgctcc
     2281 ggcggcaacg gctcccgaaa cttcaggttg agcccgcggc gaaagcgcgt cggatcttgt
     2341 cgcccgtccc cgggtggagt gaccgtcggc ggacgatcct cggcaggacg ctctttggtc
     2401 gtacgatcgg tggcgggcgg ctgagacacc tcgcggttcg tgggctcgtg ctcttgcccc
     2461 gcaacggaac cacctagcaa gaacgccgca gaagcggctg tggcggcaag aaaatcgcgg
     2521 cgatccgtct gtgccgggaa aagtgtccgc ggccgttcga tcggctggct catggctccc
     2581 tccaacattc catgccggat cgaacaatca tagatacgcg aacaccgacc gtttggcgtc
     2641 gttcgcggag cgccgatagc gaggcgcgat tcgctgagta ccagaggagg gacttgaacc
     2701 cacacgtcct taacggacac tggattttga atccagcgcg tctgccattc cgccactctg
     2761 gctcggtcag tgatttccaa ccgctcccga tttttggttc cgggcgacaa ttttgcaagc
     2821 ccccgcccat atttccgaga gaagctttac gccgcaacat ccgtgtccgc tccgtattgc
     2881 cgccgtccgg tccgtgttac gcccgtcctg tctgcgttac gcccgccccc gtttcgcaca
     2941 ccgacattta cccgtggccc ggattttcga ctcgcgatat catgtcgaac tt
//
LOCUS       DAOQMF010000148         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18656_length_2988_cov_3.413911, whole genome shotgun sequence.
ACCESSION   DAOQMF010000148 DAOQMF010000000
VERSION     DAOQMF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18656_length_2988_cov_3.413911"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLV77_03475"
     CDS             <1..1082
                     /locus_tag="PLV77_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNC14938.1"
                     /translation="LDRPDASQAWPKASSGVVIKDNRIDQVAGDGIVTRGTDGALIEG
                     NVVSHGNLAGRPLLDPEGPMCNAGIWAFRANNTVIRGNEVFGMEHNGCDGTGFDVDYR
                     QDGTVIEGNYSHDNEGGFVLLCTDTATHRADVRFNLSVDDGTMINHGPCGIADGILGD
                     LSGIRMFNNTVVGDSPTTSIQLAVSDEMYQPGRFLFRNNLVYARQPAGQIACGNDCTH
                     NAFFNLPPSGTHAVTADPQLIAGYRLGPKSPLRGAGMPITGGATTDYFGNPVPATPSI
                     GFDQTPASRPAQPTPACRKARKARDRTIERAADLNRKLRKLRSKHAPRRQTRSVASRL
                     GKARGEVKRLTRVARRACRPKGGAS"
     gene            complement(1079..2650)
                     /locus_tag="PLV77_03480"
     CDS             complement(1079..2650)
                     /locus_tag="PLV77_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC14939.1"
                     /translation="MKWITAAGVVLVCWLGLSGSAFGASLVRVTPEGYLGSDPSFVTA
                     PPGDSRVFLAERGDAGTHQARIRIIKDGVPVTEPFLTIGNVDLASERGLLSIAFAPDY
                     ASSGLFYAFYVAHGADGFGGNTGDIRIVEYKVSAGDPDQADPASARLVFSIPHSAGNH
                     NGGWMAFGPDDDLYFSIGDNATSSNAQALANYYGKIMRINPADPDGAGPATYSVPTDN
                     PFTSQAGAKKEIYALGLRNPYRASFTPDGRLVIGDVGNGTWEEVDVGDLKGANLGWPT
                     CEGFCTGLTPSFVAPIYAYNHDNGVDGQGGGCAVIGGYVVRDETLGGLTGRYLYGDLC
                     DQALRTLDLDAGGGDPQPAGFSIPGDGGNLRSFGEDAKGCVYVLSNVAVYRVAPDGST
                     APACPGTIEPPPTVTYNSYIPHQQVIGKRLIVAAKCSIACTGSATAKVRITRNRFRKE
                     PVIFNLKTPTLNLAANTRTSLVFRVPVKRVTAMKKAARNGSRVTAWVKVSLTGEDGSG
                     GSGSSSIRLVRPKRR"
     gene            complement(2661..>2988)
                     /locus_tag="PLV77_03485"
     CDS             complement(2661..>2988)
                     /locus_tag="PLV77_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14940.1"
                     /translation="QVIGKRITVAAKCSIACAASATAKVRISRNRFRRNQTVFNLKAG
                     TRNLIAGTRSSLFFRLPSARVATMKKAAGNGSRVTAWVKVSMTGEDGSGGSGSGQIRL
                     TRPVSR"
BASE COUNT          514 a         1009 c         1004 g          461 t
ORIGIN      
        1 acctcgaccg gccggacgcc agccaggcct ggccgaaagc ttcctccgga gtggtgatca
       61 aggacaaccg gatcgatcag gttgccggtg acggaatcgt caccagggga accgacggcg
      121 ccctgatcga aggcaacgtc gtaagccacg gcaacctggc cggccggccc ctgctcgacc
      181 cggaaggccc gatgtgcaac gcgggcatct gggccttccg ggccaacaac accgtgatcc
      241 ggggcaacga ggtcttcggc atggaacaca acggctgcga cggcaccggc ttcgatgtgg
      301 actaccggca ggacggcaca gtgatcgagg gcaactacag ccacgacaac gagggcggct
      361 tcgtcctgct ctgtaccgac accgcgactc accgggccga cgtgcggttc aacctgagcg
      421 ttgacgacgg cacgatgatc aaccacggtc cctgcgggat cgccgacggc atcctcggcg
      481 acctttccgg catccggatg ttcaacaaca ccgtggtcgg cgacagcccg accacctcga
      541 tccagctggc ggtatcggac gagatgtacc agccgggcag attcctcttc cggaacaacc
      601 tggtctacgc ccgtcaaccg gccgggcaga tcgcctgcgg aaacgattgc acccacaacg
      661 ccttcttcaa ccttccgccc agcggcacgc acgcggtcac cgccgacccg cagctgatcg
      721 ccggctaccg gctcggtccc aagtcaccgc tgcgaggagc cggcatgccg atcaccgggg
      781 gagccaccac cgactacttc ggcaacccgg tcccggcaac accgtcgatc ggttttgatc
      841 agaccccggc cagccggccc gctcagccga cgccggcctg ccgcaaggcc cgcaaggccc
      901 gggatcggac gatcgaaaga gcagcggacc tcaaccggaa actgaggaag ctccgttcga
      961 agcacgcgcc ccgcaggcag accagatcgg tcgcaagccg gctcggcaag gcccggggcg
     1021 aggtgaagcg actgaccagg gtcgcccgtc gtgcctgccg gcccaaaggc ggcgccagct
     1081 aacggcgttt ggggcggacc aggcggatcg aactcgagcc gctgccgccc gagccgtcct
     1141 cgccggtcag gctgaccttc acccaggcgg tcacccggct gccgttgcgg gcggccttct
     1201 tcattgccgt gacccgcttc accggcaccc ggaaaacgag gctggtgcgg gtgttggcgg
     1261 caaggttcag ggttggcgtc ttcaggttga agatcaccgg ctccttgcgg aaccggttgc
     1321 gggtgatccg gaccttcgcg gtggccgacc cggtacaggc gatcgagcac ttcgcggcca
     1381 caatgaggcg cttgccgatc acctgctggt gcgggatgta cgagttgtag gtcacggtgg
     1441 gaggcggctc gatcgttccg gggcaggccg gggccgtgga accgtcgggt gcgacccggt
     1501 agaccgcgac gttgctcagc acgtagacgc agcccttcgc gtcctccccg aaggagcgga
     1561 ggttgcctcc gtcacccggg atgctgaatc cggccggctg cggatcgcca ccgccggcat
     1621 cgaggtcgag ggtccggagc gcctggtcgc aaaggtctcc gtagaggtag cggccggtca
     1681 gccctccaag cgtctcgtcc cggacgacat aaccgccgat caccgcgcag ccaccgccct
     1741 ggccgtcgac gccgttgtcg tggttgtagg catagatcgg cgcgacgaag gagggggtga
     1801 gcccggtgca gaagccctcg caggtcggcc agcccaggtt ggcgcccttc aggtcgccga
     1861 cgtcgacctc ctcccaggtt ccgttgccga catcaccgat caccagccgg ccgtcgggtg
     1921 tgaacgaggc ccggtagggg ttgcggaggc cgagcgcgta gatctccttt tttgctccgg
     1981 cctgtgaggt gaagggattg tcggtcggta cggagtaggt ggccggcccg gccccgtcgg
     2041 gatcggccgg gttgatccgc atgatcttgc cgtagtagtt ggccagggcc tgggcgttcg
     2101 agctggtggc gttgtcaccg atcgagaagt agaggtcgtc gtccgggccg aaggccatcc
     2161 agccgccgtt gtggttgccg gccgagtgcg ggatgctgaa cacgagccgg gccgaggcgg
     2221 ggtcggcctg gtccggatca ccggccgaga ccttgtactc gacgatcctg atgtcgcccg
     2281 tgttgccgcc gaagccatcc gccccgtggg cgacatagaa ggcgtagaag agaccgctgg
     2341 atgcgtaatc gggggcgaag gcgatcgaga gcagcccccg ttcactggcc aggtcgacgt
     2401 tgccgatcgt gaggaacggc tcggtcaccg gcaccccgtc cttgatgatc cggatccggg
     2461 cctggtgggt gcccgcatcg ccacgctcgg ccaggaagac ccgcgagtca ccgggaggcg
     2521 cggtcacgaa actcggatcg gagccgagat atccctcggg tgtgacccgg accaggctcg
     2581 ccccgaaggc cgaaccgctg agccccagcc agcaaaccag cacaaccccc gccgcagtga
     2641 tccacttcat ggttacgaaa ctaccgcgaa acgggccggg tcaaacggat ctgtccgcta
     2701 cccgatcctc cggagccgtc ctcgccggtc atcgagacct tcacccaggc ggtgacgcgg
     2761 ctcccgttgc cggccgcctt cttcatcgtg gcgacccgag ccgacggcag ccggaaaaag
     2821 aggctgctgc gggttccggc aatcaggttc cgggttccgg ccttcaggtt gaagaccgtc
     2881 tggttcctcc tgaaccggtt ccggctgatc cggaccttcg cggtggccga ggcggcgcag
     2941 gcaatcgagc acttcgcggc gaccgtgatg cgtttgccga tcacctgg
//
LOCUS       DAOQMF010000149         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18847_length_2971_cov_3.334019, whole genome shotgun sequence.
ACCESSION   DAOQMF010000149 DAOQMF010000000
VERSION     DAOQMF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18847_length_2971_cov_3.334019"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1656
                     /locus_tag="PLV77_03490"
     CDS             52..1656
                     /locus_tag="PLV77_03490"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNC14941.1"
                     /translation="MSENENGTETGQNLLPGTAPLTALTKQLHERRGKARLGGGLEKI
                     EKQHAAGKLTARERIDLLVDEGSFNEIGIHGRPHYSQPAMQSKEAPADGVVTGWGDVD
                     GRPVAIVAYDFTVMAGSMGQTGEIKVTRIREMALQKRMPLVWLLDSAGARIQEAAGSL
                     FAGSGHLFREEVQMSGVVPMVAAMMGPCAAGTAYIPGLSDFVPMVVGQGAMALAGPHL
                     VKAVTGEEIEMEDLGGAKVHCRKSGVGDLEVKDDPECIESIKKYLSYFPSNCEQKPPV
                     TETADPEDRGSEKLLEIIPDSPRTPYNMYEVIEEIVDDGDWFDIKPKFAKSLITCLAR
                     MGGQPVGIVANQPKVLGGVLDVDSADKAARFINLCDAFNIPLLFLQDVPGFLVGSKVE
                     QQGIIRHGAKMLYAVSRATVPKITVLVRKAYGAGYYVMCGRAYEPDLIVAWPGAEISV
                     MGAEGAVNIIGRSAIEAADDPEATREAMLNAVRQQIDPYIAAGNAMIDDIIDPRETRQ
                     TVIRGLRMAKDKHVERPWKKHGVMPV"
     gene            1653..1988
                     /locus_tag="PLV77_03495"
     CDS             1653..1988
                     /locus_tag="PLV77_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14942.1"
                     /translation="MSEVIEVTIPHEQVQLNGVIRFDGSVVEIFGFNDVHSVRMHIQQ
                     IEFAEVKFDKGLLAQPRIMFKGKWSNLGYNQAIDPPDQIKPELENFVNAINAAIGNDA
                     ANYPNGETQ"
     gene            1985..>2971
                     /locus_tag="PLV77_03500"
     CDS             1985..>2971
                     /locus_tag="PLV77_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNC14943.1"
                     /translation="MSKFEDPVIISNSISGVIANRDQCPAIPYTPEEYAAEARRSVDE
                     GASQIHIHARTPDGTPSYEIEDFQAITEAIKAECPEVIINYSTGAIGVPIEKRIAYLE
                     ACRPDVAALNMSSMNYAKYSKRRKDFVFKAVFENSFDTIVEFITAMNDLGIKPEHECF
                     DAGHVANLDPLYDMELIKPPSQISLVMGVTGGIRPTPRNVAVMADQVPGGPEGPNNWQ
                     VIGISRDQWKLLASSLVLGGNVRAGLEDNLYLPDGDVGHDGTTRLLGGPAFGDHGRFN
                     PGDGIGMGYEDLKAIEAYNFLQSIVDGMQRAPSFADALHVAEVQTALIRSWES"
BASE COUNT          582 a          953 c          979 g          457 t
ORIGIN      
        1 aggcgtcaag gccttcttcg aaaagcgcga gccggaatgg aagggacgct gatgagcgag
       61 aacgaaaacg ggaccgagac cgggcagaac ctgctgcccg gcacggcgcc gctgaccgcg
      121 ctgaccaagc agctccacga gcggcggggc aaggcccggc tcggcggcgg cctcgagaag
      181 atcgagaagc agcatgcggc aggcaagctg accgcccgcg agcgaatcga cctgctggtc
      241 gacgagggca gcttcaacga gatcggcatc cacggacgcc cgcactactc gcagccggca
      301 atgcagtcca aggaggcgcc agccgacgga gtggtcaccg gctggggtga cgtcgatggc
      361 cggccggtcg cgatcgttgc ctatgacttc acggtgatgg ccggctcgat gggccagacc
      421 ggcgagatca aggtcaccag gattcgcgag atggcactgc agaagcggat gcctctggtc
      481 tggctgctcg actcggccgg tgcccggatc caggaagcag cgggatcgct cttcgccggt
      541 tccggccacc tcttccgcga ggaggtccag atgtccggcg tggtgccgat ggttgccgcg
      601 atgatgggtc cctgcgccgc cggcacggcc tacatccccg gtctttcgga cttcgtgccg
      661 atggtggtcg ggcagggcgc gatggccctg gccggtccgc accttgtcaa ggccgtcacc
      721 ggcgaggaga tcgagatgga ggacctcggc ggagccaagg ttcactgccg caagtccggg
      781 gtcggagacc tcgaggtgaa ggacgacccg gaatgcatcg agtcgatcaa gaagtacctg
      841 agctacttcc cgagcaactg cgagcagaag cccccggtta ccgaaacggc tgatccggag
      901 gaccgcggct cggagaagct cctggagatc atccccgatt cgcctcgcac cccgtacaac
      961 atgtacgagg tgatcgagga gatcgtcgat gacggtgact ggttcgacat caagccgaag
     1021 ttcgccaagt cgctgatcac ctgcctcgcc cggatgggcg gccagccggt cggcatcgtc
     1081 gccaaccagc ccaaggtact gggcggcgtg ctcgacgtcg attccgccga caaggcggcc
     1141 cgcttcatca acctctgcga cgccttcaac atcccgctgc tcttcctgca ggacgtgccc
     1201 ggcttcctgg tcggctcgaa ggtcgagcag caggggatca tccgccacgg ggccaagatg
     1261 ctctacgcgg tcagccgcgc caccgtgccg aagatcaccg tgctggtccg caaggcttac
     1321 ggcgccggct actacgtgat gtgcggccgg gcctacgagc ccgacctgat cgtcgcctgg
     1381 cccggagccg agatctcggt gatgggggcc gagggcgcgg tcaacatcat tggccgctcg
     1441 gcgatcgagg cggccgatga tccggaggcg acccgcgagg cgatgctgaa cgcggtccgc
     1501 cagcagatcg acccctacat cgcggccggc aacgcgatga tcgacgacat catcgacccg
     1561 cgcgaaacgc ggcagaccgt gatccgcggt ctccgcatgg caaaggacaa gcatgtcgag
     1621 cgtccctgga agaagcacgg ggtaatgccg gtatgagcga ggtgatcgag gtcacgattc
     1681 cccacgagca ggttcagctg aacggcgtga tccgcttcga cggctcggtg gtcgagatct
     1741 tcggcttcaa cgacgtccac tcggtccgga tgcacatcca gcagatcgag tttgccgagg
     1801 tgaaattcga caagggcctg ctggcccagc cgcgcatcat gttcaagggc aagtggtcga
     1861 acctcggcta caaccaggcg atcgatccac ccgaccagat caagcccgaa ctcgagaact
     1921 tcgtcaacgc gatcaacgcc gcgatcggaa acgacgccgc gaactacccg aatggagaaa
     1981 cgcagtgagt aagttcgaag acccggtgat catcagcaac tcgatctccg gggtgatcgc
     2041 caaccgggac cagtgcccgg cgattccgta cacccccgag gagtacgcgg ccgaggcgcg
     2101 ccgctcggtt gacgagggtg ccagccagat ccacatccac gcccgcaccc cggacggcac
     2161 gccctcgtac gagatcgagg acttccaggc gatcaccgag gcgatcaagg ccgagtgccc
     2221 ggaggtgatc atcaactact cgaccggcgc gatcggcgtg ccgatcgaga agcggatcgc
     2281 ctacctcgag gcctgccgcc ccgatgtcgc cgcgctcaac atgagctcga tgaactacgc
     2341 caagtactcg aagcgccgga aggacttcgt cttcaaggcg gtcttcgaga actccttcga
     2401 cacgatcgtc gagttcatca ccgcgatgaa cgacctcggc atcaagccgg agcacgagtg
     2461 ctttgacgcc ggtcacgtcg ccaacctcga tccgctctat gacatggaac tgatcaagcc
     2521 gccctcccag atctccctgg tcatgggtgt gaccggcggc atccggccga ccccgcgcaa
     2581 cgtggcggtc atggccgacc aggtgccggg cggccccgag ggcccgaaca actggcaggt
     2641 gatcggcatt tcccgcgacc agtggaagct gctcgcgagc tcgctggtgc tcggcggcaa
     2701 cgtccgtgcc gggctcgagg acaacctcta cctgcccgac ggcgatgtag gccatgacgg
     2761 gacgacgcgg ctgctaggtg ggccggcgtt tggcgaccat ggccggttta acccaggcga
     2821 cgggatcggg atggggtacg aggatctcaa ggcaatcgag gcgtacaact tcttgcagtc
     2881 tatcgtggac ggcatgcagc gggcgccgtc atttgccgat gcgctccacg tggcggaggt
     2941 gcagacggca ttgatacggt cgtgggaaag c
//
LOCUS       DAOQMF010000150         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18938_length_2963_cov_3.676066, whole genome shotgun sequence.
ACCESSION   DAOQMF010000150 DAOQMF010000000
VERSION     DAOQMF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18938_length_2963_cov_3.676066"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLV77_03505"
     CDS             <1..710
                     /locus_tag="PLV77_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNC14944.1"
                     /translation="SQALMLFLAVATLVLPAVFVLVHGGGLPHVEEEITDFGSDVNTI
                     SIVASAILLFTYAAGLLFSLRTHKSFFNPSGEEHGSPLGWSVRKAVGMLAFAGLAVGV
                     MAHILVGSLEEVSVSLGLSEFFVGIIIVAIVGNAAESWVSILYATKNKMDLSVQIAIG
                     SATQLALVVMPTLVIVSFFLGPNPMALVFNGYEMASLVIAVMAAYYVVQEGESNWFEG
                     LQLLALYTVLGVVFYFA"
     gene            complement(712..1047)
                     /locus_tag="PLV77_03510"
     CDS             complement(712..1047)
                     /locus_tag="PLV77_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNC14945.1"
                     /translation="MSFGSVYPHYVAKAEKKGRSKDEVDEIIRWLTGYDQEQLESQIE
                     KGVDIETFYGEAPELNPSRELITGVVCGVRVEEIEEPLMQEIRYTDKLIDELARGKSM
                     GKILRAPAG"
     gene            1157..1879
                     /locus_tag="PLV77_03515"
     CDS             1157..1879
                     /locus_tag="PLV77_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNC14946.1"
                     /translation="MRGLLATLLVSVVALTALSGCGTANSSNEPELPALPPIQHDLGP
                     VAYRELIGDTFELLNEYWNEQANRLGETMPPPRALVSFWTRAQDKGCGGEKLGWQNAQ
                     YCWQNRTIAWDGNWVYGVLYRRLGDAAVAFLLGHEYGHMVQDELGVSSEFRHTIEAEL
                     NADCLSGAWLGAVNDQVITFDRSDYESLYGGMLEVADAYGVPWTDPSAHGTAAQRGRA
                     LLFGARHGARACINQLGPGFTA"
     gene            complement(1883..2458)
                     /locus_tag="PLV77_03520"
     CDS             complement(1883..2458)
                     /locus_tag="PLV77_03520"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNC14947.1"
                     /translation="MDTAFLEELIEHKVAPEPLIAIQGLLNTHDYERDEELIGDAASA
                     RVWLAGADLIEEEATVSDEEVRDLIELRKILRRILESRTKGDEVDPGLYRDLQLLANK
                     YPAGYDVSDRGEVTLCLKPVETAGELIGQLVGIVGIAQENDWWERLKICADDDCRWAF
                     YDASKNRGGTWCRMEVCGNRNKNRRYRSKTN"
     gene            2561..2722
                     /locus_tag="PLV77_03525"
     CDS             2561..2722
                     /locus_tag="PLV77_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14948.1"
                     /translation="MFPFATLFARRSRPRTDLRTSVLDEEYEWVFGRKPSNELRQHRE
                     RPGERQDAE"
     gene            complement(2741..>2963)
                     /locus_tag="PLV77_03530"
     CDS             complement(2741..>2963)
                     /locus_tag="PLV77_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14949.1"
                     /translation="TFQVNHFSINHRARLGGYQVSGARWHGPCHHGLCTSGQWVNPNK
                     IQGHWWHENSPAHKVGYVVYWVNGMSGPG"
BASE COUNT          485 a          971 c          885 g          622 t
ORIGIN      
        1 catcccaggc cctgatgctc tttctcgcgg tggcaaccct ggttctgccg gcggtcttcg
       61 tgctggtcca cgggggcggc ctcccccacg tcgaggagga gatcaccgac ttcggctcgg
      121 acgtcaacac gatctcgatc gtcgcttcgg cgattctgct cttcacctac gccgccgggc
      181 tgctcttctc gctcaggacc cacaaaagct tcttcaaccc gtccggcgaa gaacacggtt
      241 cgccgctcgg ctggagcgtc cgcaaggcgg tcggcatgct cgccttcgcc ggtctcgcgg
      301 tcggcgtgat ggcccacatc ctggtcgggt cgctcgagga ggtttcggtt tcgctcgggc
      361 tgtccgaatt ctttgtcggc atcatcatcg tcgcgatcgt cggcaacgcc gcggagagct
      421 gggtgtcgat cctctacgcg acgaagaaca agatggacct ttcggtccag atcgcgatcg
      481 gttcggcgac ccagctcgcc ctggtcgtga tgccgaccct ggtgatcgtc tccttctttc
      541 tcggcccgaa cccgatggcc cttgtcttca acggctacga gatggccagc ctggtcatcg
      601 cggtcatggc cgcctactac gtggttcagg aaggcgaatc gaactggttc gaaggcctcc
      661 agctgctcgc cctctacacc gttctcggcg tcgtcttcta cttcgcctga gctacccagc
      721 gggagcgcgg agaatcttcc ccatcgactt gccccgggcg agttcgtcaa tcagcttgtc
      781 ggtgtagcgg atctcctgca tcagaggttc ttcgatttcc tcgacccgta ccccgcagac
      841 caccccggtg atcagctccc gtgaaggatt gagctcgggt gcctctccgt agaacgtttc
      901 gatgtcgacc cccttctcga tctgcgactc gagttgctcc tggtcgtaac ccgtcagcca
      961 gcggatgatc tcgtcgactt cgtccttgct cctccccttc ttctccgcct tcgccacata
     1021 atgcggatag acgctgccga aactcatcct gtaaatgcgg tgaccggtca agactctcct
     1081 cgctggtttc gaggatccta gtcacggaga tacaaaatga tccgcgctcg gggagccatg
     1141 tgcgacgatc ggagagatga gggggctcct cgccacactg ctggtttcgg tggttgcctt
     1201 gaccgctctg tcgggctgcg gtaccgcgaa ctcctcgaat gagccggaac tgcccgcctt
     1261 gccgccgatc cagcatgacc tcgggccggt tgcctaccgt gagttgatcg gtgacacttt
     1321 cgaattgctc aacgagtact ggaacgagca ggcaaacagg cttggcgaga cgatgccgcc
     1381 tccgcgggcg ctggtttcgt tctggacccg ggcccaggac aagggctgcg gcggcgagaa
     1441 gctcggctgg cagaacgccc agtactgctg gcagaaccgg accatcgcct gggacggaaa
     1501 ctgggtctac ggggttctgt accggcgcct cggcgatgcc gcggtggcat tcctgctcgg
     1561 ccacgagtac gggcacatgg tccaggacga gctcggagtt tccagcgagt tccgccacac
     1621 gatcgaggcg gaactgaatg ccgattgttt gtccggggcc tggctcggtg cggtgaacga
     1681 ccaggtgatt accttcgacc gttccgacta cgaatccctc tacggcggaa tgctcgaggt
     1741 cgccgacgcc tacggggttc cgtggaccga cccatccgcc cacggtaccg ctgcccagcg
     1801 cggacgggcc ttgctgttcg gagcccgcca cggagcccgg gcctgcatca accagctcgg
     1861 ccccggcttc accgcctgaa cttcagttcg tcttgctgcg gtagcggcgg ttcttgttgc
     1921 ggttgccgca gacttccatc cggcaccagg tcccgccgcg gttctttgag gcgtcgtaga
     1981 aggcccaccg gcaatcgtcg tcggcgcaga tcttcaggcg ctcccaccaa tcgttctcct
     2041 gggctatgcc gactatgccg accagctggc cgatcagctc acccgcggtt tcgaccggtt
     2101 tcaggcaaag ggtgacttca ccccggtcgc tcacgtcata gccggccgga tacttgttgg
     2161 cgagaagctg cagatcgcgg tagagacccg gatcgacctc gtctcccttc gtccgtgact
     2221 cgaggatgcg ccgcaggatc ttgcggagtt cgatcagatc ccgaacctcc tcgtcgctga
     2281 ccgtcgcttc ctcctcgatc agatccgcgc cggccagcca tacccgtgcg gatgcggcgt
     2341 caccgatcag ttcctcatcc cgttcgtagt catgcgtgtt gagcagaccc tggatcgcga
     2401 tcagcggttc cggcgcgacc ttgtgttcga tcagctcttc gaggaaggcg gtgtccatgg
     2461 atccatcgta ctcctgcgtg accggtaaat caacttgaca ggtcactcta accggtgtaa
     2521 cgtttttacc agttagcaag aattcaactg aaaggaagcc atgttcccct tcgcgacact
     2581 cttcgccagg cgcagcaggc cgcgcaccga cctccggacc tcggttctcg acgaggaata
     2641 cgaatgggtc ttcggccgca agccttcgaa cgagctgcgg cagcaccgcg agcggccggg
     2701 cgagcggcag gacgccgagt agcccggacg gactgtcctg ctatccggga cctgacatcc
     2761 cgttcaccca gtagacgacg tagccgacct tgtgcgcggg tgagttttcg tgccaccagt
     2821 ggccctggat cttgttcggg ttgacccact ggccgctcgt gcagagcccg tggtgacagg
     2881 ggccatgcca gcgggcgcct gatacctggt atcccccgag cctggcccgg tgatttatcg
     2941 agaagtgatt gacctggaag gtg
//
LOCUS       DAOQMF010000151         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_18973_length_2960_cov_3.218244, whole genome shotgun sequence.
ACCESSION   DAOQMF010000151 DAOQMF010000000
VERSION     DAOQMF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18973_length_2960_cov_3.218244"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1939)
                     /locus_tag="PLV77_03535"
     CDS             complement(173..1939)
                     /locus_tag="PLV77_03535"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNC14950.1"
                     /translation="MFSKILIANRGEIAIRVARACKEMGITSVAVYSEADRDAPHVKA
                     CDEAFLIGPAVPAESYLSIEKIIQAAKDSGAEAVHPGYGFLAENADFARALEDAGIVF
                     IGPPASAIDAMGSKTMAREVMEKAGVPIVPGATEPAKDLDEARAQAEAAGYPVACKAV
                     GGGGGKGFRVAMTPDDLQEAFEGSGREGMKFFNDDRVYIERYLEDPRHVEVQVLADKH
                     GNVIHLAERDCSIQRRHQKLIEEAPGPHVDEEMRERIGKIATDAARAVGYFSAGTIEG
                     MQHGDEYFFLEMNTRVQVEHCVTEEITGVDIVREQIKIAYGEELSLKQEDVKIDGWAI
                     ECRINAEKAHMNFAPAPGPIEVYDEPSGPGVRVDSGVKAGSEVTPMYDPMVAKLIVWD
                     KDREAATKRMLRALDEYEIAPLSTLIPFHKTILRTEQWARGETCKDLTEDKKWLKTTA
                     PEDTSIPEPADGEAEKVARDYLVEVSGKRFDVKVIGEATGFAAAGAAPGAGGPKPKRE
                     KRAGGGGGGGGPVLASPLQGSIFKLEVAEGAEVAEGDLICVIEAMKMENEITAHKAGK
                     VTKVSISVGDAVASGDPLVTIE"
     gene            2208..>2960
                     /locus_tag="PLV77_03540"
     CDS             2208..>2960
                     /locus_tag="PLV77_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14951.1"
                     /translation="MNSVSGESPSSAIELDRLWQFMSRRRHVAGRDELIRHGFSPSRI
                     KNWLRGRRLTKLFHGIYAYGRDIETRESAWRAALLVAGPGSALTSRSALELLGAARLL
                     TPIPPVIHVATSRDRAGRHRGLSPALRNTWVYVVRRRFDPGDLRERGGLAVVRVALAL
                     LDFAVSAPDTDVKFAFLELCRLRLFGEKDVQFCFRRSVGKRGALKVRPLLGLWVPELN
                     RIKSVLEGLFLLSWVPRRWKLPEVNVKIFGREV"
BASE COUNT          465 a          957 c          954 g          584 t
ORIGIN      
        1 cgaaagtgcg aatggcggcg ccgattccgg caactccggc cagaactgcg gcgagggcag
       61 cgctgacgct gccggggtcc agataggcaa aaatgtgcat tcgtgatcct ctcgtgggcg
      121 ggatcgaccg ttgcctgact ttcctgagca ggccccgacc gaccgcgaaa tgttactcaa
      181 tcgtgaccag agggtccccg gacgcgacgg catcgccaac ggaaatgctg accttggtga
      241 cctttccggc cttgtgagcg gtgatctcgt tttccatctt catcgcttcg atcacgcaga
      301 tcaggtcgcc ttcagccacc tcggcgcctt cggcgacttc gagcttgaag atcgatccct
      361 ggagcggtga ggcaagcacg gggccgcctc cgccgcctcc gccaccggcg cgcttctcgc
      421 gcttcggctt gggtcctccg gctccgggcg cggcgccggc ggcggcaaag ccggtcgctt
      481 cgccgatcac cttgacgtca aagcgcttgc cggaaacctc gaccaggtag tcgcgggcaa
      541 ccttttcggc ctcgccgtca gccggctcgg ggatcgaagt gtcctcgggg gccgtggtct
      601 tcagccactt cttgtcttcg gtcaggtcct tgcaggtctc gccacgagcc cactgttcgg
      661 tccggaggat cgtcttgtgg aacgggatca gggtcgagag cggcgcgatc tcgtactcgt
      721 cgagcgcgcg cagcatccgc ttggtcgcgg cctcgcggtc cttgtcccag acgatcagct
      781 tggcgaccat cgggtcgtac atcggggtga cctcggaacc ggccttgacg ccggaatcga
      841 cccggacgcc cggaccggaa ggctcgtcgt agacctcgat cgggcccgga gcgggggcga
      901 agttcatgtg ggccttctcc gcgttgatgc ggcattcgat cgcccagccg tcgatcttca
      961 cgtcttcctg cttgaggctc agttcctcgc cgtaggcgat cttgatctgc tcccggacga
     1021 tgtcgacccc ggtgatctcc tcggtcacgc agtgctcgac ctggacccgg gtattcatct
     1081 cgaggaagaa gtactcgtcg ccgtgctgca tgccttcgat cgtgccggcc gagaagtaac
     1141 cgacggcgcg ggccgcgtcg gtcgcgatct tgccgatccg ctcgcgcatc tcttcgtcca
     1201 catgcgggcc gggggcctct tcgatcagct tctggtgccg gcgctggatc gagcagtcgc
     1261 gctcggcgag gtggatcacg ttgccgtgct tgtcggccag cacctggact tcgacgtggc
     1321 gcgggtcctc caggtagcgc tcgatgtaca cgcggtcgtc gttgaagaac ttcattcctt
     1381 cgcggccgga gccctcgaag gcctcctgca gatcgtccgg ggtcatggcg acgcggaagc
     1441 ccttgccgcc gccgccaccg accgccttgc aggcgaccgg gtagccggcc gcttcggcct
     1501 gggcgcgggc ctcatcgagg tccttggcgg gctccgtcgc gccgggcacg atcggcacgc
     1561 cggccttctc catcacttcg cgggccatcg tcttcgaacc catcgcgtcg atcgccgagg
     1621 cgggcgggcc gatgaaaact atcccggcat cttccagtgc ccgggcgaag tccgcgttct
     1681 cggccaggaa gccgtagccg gggtgaaccg cctcggcccc ggagtccttc gcggcctgga
     1741 tgatcttctc gatcgagagg taactctcgg ccggaaccgc cggtccgatc aggaaagctt
     1801 cgtcacaggc tttgacgtgc ggagcatccc gatccgcctc cgaataaacg gcgacggagg
     1861 taatccccat ctccttgcaa gcccgggcga cacggatagc gatttcgcca cgattggcga
     1921 tcaggatctt cgaaaacata gcccgggcag tctatttatc cgccccggtc ccggctttgc
     1981 ccccgccccc tcccagccag cccgcgcaga gccatccgat ccaagccagc cccagaatcg
     2041 agaaggcaaa agtggccata tagcgctcca tttagccttc tcggcggtgg cggctcgaac
     2101 cggcgagttc aaggcaggct tcgcgggcgt ttcaggctcc cgggacgttg cacaattgtc
     2161 acccttatag agccacgtgt gagcatttct cgggcaggct tgggccgatg aattcggtct
     2221 ccggggaaag tccatccagc gccatcgagc ttgaccggct ttggcagttc atgtcccggc
     2281 gccgtcatgt tgccgggagg gatgaactca ttcgtcatgg attcagtccg tccaggatca
     2341 agaattggct cagaggcagg cggttgacca aactcttcca cggcatctat gcctacgggc
     2401 gggacataga gaccagagag tcggcctggc gggcggctct cctggttgcg ggtcccggtt
     2461 ctgccctgac ctcacggagt gccctggagc tcctgggggc ggcgcgcctc ttgactccga
     2521 tcccgccagt gatccacgtg gctacatcca gagaccgcgc tggcaggcac cgagggctgt
     2581 cgcccgctct caggaacacc tgggtctatg tggtccgccg acggttcgac ccgggtgacc
     2641 tgcgcgagag aggcggcctt gcggtcgtgc gagtagcttt ggccctgctc gactttgccg
     2701 taagtgctcc tgacacagac gtgaaattcg ctttcctgga gttgtgccga ctccggttgt
     2761 ttggcgaaaa ggacgtgcag ttctgcttcc gccggtcagt gggaaaacga ggcgcgctaa
     2821 aggtgaggcc gttgctcggg ttgtgggtgc cggaactgaa ccggatcaag tccgtactcg
     2881 aggggctgtt tctcctgtcg tgggtgccgc gacggtggaa gctgccggag gtgaacgtga
     2941 agatcttcgg gcgagaggtc
//
LOCUS       DAOQMF010000152         2955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19025_length_2955_cov_3.195517, whole genome shotgun sequence.
ACCESSION   DAOQMF010000152 DAOQMF010000000
VERSION     DAOQMF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19025_length_2955_cov_3.195517"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /gene="nhaA"
                     /locus_tag="PLV77_03545"
     CDS             complement(<1..1105)
                     /gene="nhaA"
                     /locus_tag="PLV77_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNC14952.1"
                     /translation="MKPLHDPDTTESRRDLAAQVAKPMRQFLATESGSAGLLLLAGVA
                     ALIWSNAPFSDSYFSFWSTELSVKVGDLSIAHDLRYWIDEALMAMFFFVIGMEVRREM
                     AMGELTDRKRVLIAVMGGIGGMIFPALIYLAINSGSGHEHGWGMVIATDTAFVLGALA
                     IVGPKFPTQLRVFLLTLAIVDDIVAITVIGLFYSEALDYTAIAICVAALFLIWLYPRI
                     GVWRSGIYFATVGVAVIAAMESGFHATLAGMIAGLLVAAYPPQPKKVEKAARRVRAFR
                     QSPLPEVARSANLSVQQALSPNERFQTVFHPFTSYVVVPLFALANAGVDLGGGVLGDA
                     LGSTLTWGVVAGLVLGKTLGITTGVVTAIRTRIG"
     gene            complement(1232..>2955)
                     /gene="purL"
                     /locus_tag="PLV77_03550"
     CDS             complement(1232..>2955)
                     /gene="purL"
                     /locus_tag="PLV77_03550"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNC14953.1"
                     /translation="FEPPYEQNPLVNAMCVGLARTDEMVRAAAAGAGNKVVLFGSLTG
                     RDGIGGASVLASAELDEGDDAKRPTVQIGDPFEESKLLECCQELLAKDLLVSLQDLGA
                     AGLTSSAGEMASAGGTGIDIDVGLVPLREADMEPFEIMVSESQERMLAVVEPGKVEQV
                     LALCTKWQTASAVIGEVTDSGTFRILKDGETVGEIRVEDLVDGCPLYDLDPGDPGEWT
                     YPNRETIPADASASDIALALLASPSIASKRWAFEQYDSIVGSRTAHRAGAADAAVLNI
                     PESGRAIAVAIDGNGRRVACDPYQGTIEATLECAANLAVVGAEPLGVTNCLNFGNPEK
                     PTVAWQLGRSIDGLAEACRALEAPVVGGNVSLYNETPQGPIYPTPVVGMVGELPDPSR
                     VAGEPWREGDAIALVGPFDPSLAGSELEKQRGQLGKGLPEFEIAPVRQAIELVREAVR
                     SGSIRTATDVSDGGIATALAELAIGSGVGCDAHLTALLAKRDCSAEDALFGEGPGGFI
                     LAGEEAGLEKLGGRGVEIEVIGEVRGNEVIVTASDRRVEILVSEAEAAFESLEERIES
                     DQAGITH"
BASE COUNT          497 a          991 c         1004 g          463 t
ORIGIN      
        1 cgccgatcct ggtcctgatt gccgtcacca caccggtcgt gatgccgagc gtcttgccca
       61 gcacgagacc ggccaccacg ccccaggtca gggtcgaacc gagcgcatcg ccgagcaccc
      121 cgccgccgag gtccaccccg gcgttggcca gcgcgaagag cggcaccacc acgtaactcg
      181 tgaaggggtg aaagacggtc tggaaccgtt cgtttggcga gagcgcctgc tgaacgctga
      241 ggttggccga gcgggccacc tcgggcaggg gcgactggcg gaaggcccgg acccgccggg
      301 cggccttctc gaccttcttc ggctggggcg ggtaagcggc gaccagcagc ccggcgatca
      361 tcccggccag ggtcgcgtga aagccggatt ccatggctgc gatcaccgcc accccgacgg
      421 tcgcgaagta gatccccgat cgccagaccc cgatccgcgg gtacagccag atcagaaaga
      481 gcgcggcgac gcagatcgcg atcgcggtgt agtccagcgc ctccgaatag aagaggccga
      541 tcacggtgat cgcgacgatg tcatcgacga tcgccagggt gagcaggaag acccgcagct
      601 gggtcgggaa cttcggaccg acgatcgcca gcgccccgag cacgaaggcg gtgtcggtgg
      661 cgatcaccat gccccagccg tgctcgtggc cggaaccgga gttgatcgcc aggtagatca
      721 gggcggggaa gatcatcccg ccgatgccgc ccatcacggc gatcagcacc cgcttgcggt
      781 cggtcaactc gcccatcgcc atttcccgtc ggacctccat gccgatcacg aagaagaaca
      841 tcgccatcag cgcctcgtcg atccagtacc tcaggtcgtg tgcgatcgag aggtcgccga
      901 ccttgaccga gagttccgtg ctccagaagc tgaagtacga gtcgctgaaa ggtgcgttcg
      961 accagatcag cgcggcgacg ccggccagca gcagcagccc ggcgctgccg gattcggtcg
     1021 ccaggaactg gcgcatcggc ttcgcgacct gggccgcaag atcgcgcctg ctctcggttg
     1081 tatcgggatc gtgaaggggc ttcaccaggt ccctgttgaa cctaccgggt cagcatgttg
     1141 gtctcaccag cggtcggagc ccgttcatcc gtcatttctt tctgtgaacc ggcgggtttt
     1201 acttggaggc ggagcgcagc tcaccgacta cttaatgcgt gatgcccgcc tgatctgact
     1261 cgatgcgctc ctcgagggat tcaaaggcgg cttcggcttc gctgacgagg atttccacgc
     1321 ggcggtcgga ggcggtgacg atcacctcgt tgccccggac ctcaccgatc acctcgatct
     1381 cgacgccgcg gccaccaagc ttctccagcc cggcttcctc gccggcgagg atgaagcccc
     1441 cggggccctc gccgaagagg gcatcctccg ccgaacagtc gcgcttggcg agcaatgcgg
     1501 tcaggtgggc gtcgcagccg actccggagc cgatcgccaa ttcggccagg gcggtggcga
     1561 taccgccgtc ggaaacatcg gtggcggtgc ggatcgaacc ggaacggacc gcttcccgga
     1621 ccagttcgat cgcctgcctt accggggcga tctcgaactc cggcaggccc ttgccgagct
     1681 ggccgcgctg cttctcgagc tcggaaccgg ccagcgaggg gtcgaacggg ccgaccaggg
     1741 cgatcgcatc accctcgcgc cagggctcac cggccacccg ggacggatcg ggcagctcgc
     1801 cgaccatgcc gaccaccggg gtcgggtaga tcgggccctg cggagtctcg ttgtagagcg
     1861 agacgttgcc gccgaccacg ggcgcctcga gcgcgcggca ggcctcggcc aggccgtcga
     1921 tcgagcgacc gagctgccag gcgacggtcg gcttctccgg attgccgaag ttgaggcagt
     1981 tggtgacgcc gagcggctcg gccccgacca cggcgaggtt ggccgcgcat tcgagcgtgg
     2041 cttcgatcgt gccctgatag ggatcgcagg cgacccggcg gccgttgccg tcgatcgcga
     2101 ccgcgatcgc ccggccggac tcgggaatgt tgaggacggc agcgtcggcg gcaccggccc
     2161 ggtgggcggt gcgcgaaccg acgatcgagt cgtactgctc gaaggcccag cgcttggagg
     2221 cgatcgaagg cgaggcgagc agggccagcg cgatgtcact ggccgaggca tcggcaggga
     2281 tggtttcgcg gttgggatag gtccactcgc ccgggtcccc tgggtcgagg tcatagagcg
     2341 ggcagccgtc gaccaggtcc tcgacccgga tctcgcccac cgtttcgcca tctttcagga
     2401 tgcggaaggt gccggaatcg gtgacctcgc cgatcaccgc ggaggcggtc tgccacttgg
     2461 tgcagagcgc gagcacctgc tcgaccttgc ccggctcgac cacggccagc atccgttcct
     2521 gtgattcgga aaccatgatc tcgaagggct ccatgtcggc ttcccggagc ggaaccagtc
     2581 cgacgtcgat atcgatcccg gtccctccgg cggaagccat ctcgccggcc gaggaggtga
     2641 gcccggcggc accgaggtcc tgaagggaaa cgagcaggtc tttggccagt agttcctggc
     2701 agcactcgag cagcttcgac tcctcgaagg ggtcgccgat ctggacggtc gggcgctttg
     2761 cgtcatcgcc ttcatcgagt tcggccgagg ccagcaccga ggcgccgccg atgccgtcgc
     2821 gaccggtcag cgaaccgaag agcacgacct tgttgccggc cccggccgcg gccgcccgga
     2881 ccatctcgtc ggtgcgggca aggccgacac acatcgcgtt gaccagcggg ttctgttcgt
     2941 agggtggctc gaagt
//
LOCUS       DAOQMF010000153         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19088_length_2950_cov_3.563040, whole genome shotgun sequence.
ACCESSION   DAOQMF010000153 DAOQMF010000000
VERSION     DAOQMF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19088_length_2950_cov_3.563040"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /gene="leuS"
                     /locus_tag="PLV77_03555"
     CDS             complement(<1..1421)
                     /gene="leuS"
                     /locus_tag="PLV77_03555"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNC14954.1"
                     /translation="MSRYEPREIEGKWQQVWADEGTWEVPNPGQEGFDPEKPKSYVLE
                     MLPYPSGEPHVGHLKCYSLGDAIAHFRRRNGFEVIHPMGFDSFGLPAENNAIKTGEPP
                     LVATERSIESFRKQFADWGVSIDWSREVAPCTPEYYRWTQWIFLQLFERGLAYRTQAP
                     VQWCPKDATVLANEQVVDGRCERCGTQVETRNLRQWFFKTTEYAQQLLDDFDELEAWP
                     PHVITMQKNWIGRSEGAEVTFRCESANLDFPVFTTRPDTLFGATFFVMAPEHPELDRL
                     IEGTEVEDQVREYVNSVARESVAERGDEGRPKTGVPLGRTVTNPVNGEEIPMFVADYV
                     LMDYGTGAIMAVPGHDQRDYEFAKVFDLPVRRVIEGEPEEGGDDRGLPYAGDGPMTGS
                     GRFDGMNNRAAYDEIVEWLKLEGRGDASVNFRLRDWLVSRQRYWGAPIPIVYCDECGM
                     VPVPEEQLPVRLPAPDQYAARPMR"
     gene            complement(1445..2437)
                     /locus_tag="PLV77_03560"
     CDS             complement(1445..2437)
                     /locus_tag="PLV77_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC14955.1"
                     /translation="MTDLSLNGVHHISCITGDAPGNVEFYAGLLGMRLVKKTVNQDDP
                     SVYHLFYGDEKGSPGFDLTFFEYPGSREGRAGAGMIHRILWRVGSPDSLDYWAARLGQ
                     AGVTTVRSSDEGASFYGIGPTGEGDTLLFQDPEGLTHEILVYDGPDAPLIPGHVDIPA
                     EHAIQGFHGARAFSGNPEVTRQVLGQVLGFTDEGEDHWHLAGEAGDGFEARNGYYFLD
                     EPPADRAIPGAGTVHHIAWASPMDQHDEWREKVAATGLYITPVIDRFYFKALYFREPG
                     GILFEIATIGPGFATDEDPEHLGERLSLPPDFESVREQVEPNLRPIPDITQWRP"
     gene            2489..2935
                     /locus_tag="PLV77_03565"
     CDS             2489..2935
                     /locus_tag="PLV77_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC14956.1"
                     /translation="MGILEASRVVEIDAPKSECYAIIADLPATPEWQESMISLDVIET
                     NSEGFASLVEIKSDGKVKEVTSLIAFEYQPEEGMSWEQKKGDMKWLKGKWILEELEEG
                     RTRATYWLKADTGRMLGLLIKGPVQDKLKEWLTKDAAEGLKARAES"
BASE COUNT          509 a          955 c          973 g          513 t
ORIGIN      
        1 cgcatcggcc ttgccgcata ttggtccggc gcgggcaacc ggaccggcaa ctgctcttcg
       61 ggcaccggga ccatgccgca ttcatcgcag tagacgatcg ggatcggcgc gccccagtac
      121 cgctgccgcg acacgagcca gtcacgcagg cggaagttga ccgaggcgtc gccccggcct
      181 tcgagcttca gccactcgac gatctcgtcg taggccgccc ggttgttcat gccgtcgaaa
      241 cggccggaac cggtcatcgg accgtcgccc gcgtagggca gaccccggtc gtctcccccc
      301 tcctccggtt cgccctcgat cacccttctc accggcaggt cgaagacctt cgcgaactcg
      361 tagtcgcgct ggtcgtggcc cggcaccgcc atgatcgcgc cggtgccgta gtccatcagc
      421 acgtagtcgg cgacgaacat cgggatttct tcgccgttga caggattggt caccgtgcgg
      481 ccgagcggca ccccggtctt gggtcggccc tcgtcaccgc gctcggcgac cgactcgcgg
      541 gcgaccgagt tcacgtactc ccggacctgg tcttcgacct cggtgccttc gatcaaccgg
      601 tcgagctcgg gatgttcggg tgccatcacg aagaaggtcg cgccgaagag cgtgtcgggc
      661 cgggtcgtga agaccgggaa gtcgaggttg gccgactcgc agcggaaggt cacctcggcg
      721 ccttcggagc ggccgatcca gttcttctgc atggtgatca cgtgcggcgg ccaggcctcg
      781 agctcgtcga agtcgtcgag cagctgctgg gcgtattcgg tggtcttgaa gaaccactgc
      841 ctcaggttgc gggtctcgac ctgggttccg caacgctcgc agcggccgtc gaccacctgc
      901 tcgttggcca gcaccgtcgc gtccttcggg caccactgga ccggtgcctg ggtccggtag
      961 gcgagcccgc gctcgaacag ctgcaggaag atccactggg tccagcggta gtactcgggg
     1021 gtgcagggcg cgacctcccg ggaccagtcg atcgagaccc cccagtcggc gaactgcttg
     1081 cggaacgact cgatcgagcg ctcggtcgcg accagcggcg gctcgccggt cttgatcgcg
     1141 ttgttctcgg ccggcagacc gaacgagtcg aagcccatcg gatgaattac ctcgaaaccg
     1201 ttccggcgcc ggaaatgggc gatcgcatca cccagcgagt agcacttgag gtgcccgacg
     1261 tggggttcac ccgaggggta gggcagcatc tcgagcacgt agctcttggg cttctcgggg
     1321 tcgaagcctt cctgaccggg attcggcacc tcccaggtgc cctcgtccgc ccagacctgc
     1381 tgccatttgc cctcgatttc gcgaggttcg taccgactca tcggcgcgga gtctatttct
     1441 caggctaggg tcgccactgc gtgatgtcgg ggatcggcct gaggttcggc tcgacctgct
     1501 cgcggaccga ttcgaaatcg gggggcagcg agagccgctc gccgagatgc tccgggtcct
     1561 cgtcggtggc gaaaccgggg ccgatcgtgg cgatttcgaa gaggatgccg cccggctcgc
     1621 gaaagtagag cgccttgaag tagaagcggt cgatcaccgg ggtgatgtag agcccggtcg
     1681 cggcgacctt ctcccgccac tcgtcgtgct ggtccatcgg cgaggcccag gcgatgtgat
     1741 gcaccgttcc ggcgcccggg atcgcccggt ccgccggcgg ctcgtcgagg aagtagtagc
     1801 cgttgcgggc ctcgaaaccg tcacccgcct caccggccag atgccagtga tcctcgcctt
     1861 catcggtgaa gccaagcacc tgtccgagca cctgcctggt cacttcgggg ttgccggaga
     1921 aggcgcgggc tccgtggaag ccctggatcg catgctcggc cgggatgtct acgtggcccg
     1981 ggatcagcgg cgcatccggt ccgtcgtaga cgaggatctc gtgggtcagc ccttccgggt
     2041 cctgaaacaa cagggtgtcg ccctcaccgg tcggaccgat cccgtagaag gaggcgcctt
     2101 cgtcggaact gcggaccgtc gtgaccccgg cctgcccgag cctcgcggcc cagtaatcga
     2161 gtgaatcagg ggagccgacc cgccagagaa tgcggtgaat catcccggct cccgcccgcc
     2221 cctcgcgcga tccggggtat tcgaaaaagg tcaggtcgaa accgggcgag cccttctcgt
     2281 caccgtagaa gaggtggtag acggaggggt cgtcctgatt gaccgtcttc ttgaccagcc
     2341 gcatgccgag caggccggcg tagaactcga cgtttcccgg tgcgtcgccg gtgatgcagc
     2401 tgatgtgatg aacgccgttc aggctgagat cggtcatgaa gccaagcgta cttggctggc
     2461 atccccggtc cgggatagca tcccgaacat gggaatcctt gaagcttcac gcgtcgtgga
     2521 gatcgacgcc cccaagtccg agtgctacgc gatcattgcc gaccttcccg ccactcccga
     2581 gtggcaggaa tcgatgattt cgcttgacgt catcgagacg aattccgagg gcttcgcctc
     2641 gctcgtggaa atcaagtccg acggcaaggt caaggaagtc acttcgctga tcgccttcga
     2701 ataccagccc gaggaaggca tgagctggga acagaagaag ggcgacatga aatggctgaa
     2761 gggcaagtgg atcctggagg agctcgagga aggcaggacc cgcgccacct actggctcaa
     2821 ggccgacacc ggtcgcatgc tgggcctgct gatcaagggt ccggtccagg acaagctgaa
     2881 ggaatggctg accaaggacg cagccgaagg cctcaaggcc cgcgccgaga gctagacagc
     2941 cgcgatcagg
//
LOCUS       DAOQMF010000154         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19090_length_2950_cov_3.042832, whole genome shotgun sequence.
ACCESSION   DAOQMF010000154 DAOQMF010000000
VERSION     DAOQMF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19090_length_2950_cov_3.042832"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLV77_03570"
     CDS             <1..962
                     /locus_tag="PLV77_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HNC14957.1"
                     /translation="GQDTERGTFSQRHLVLHDEKTGIKYAPIQHLKDATAPFELHNSP
                     LSEIACLGFEYGYASSNPDSLVIWEAQFGDFANSAQVIIDSFIASGEAKWGMTSRLTM
                     LLPHGHEGAGPEHSSARMERFLQLAAEGSMRVANPSTAAQYFHLLRRQALIKKARPLV
                     VFTPKGLLRQTAATATLEQLTGEHFHFILDDPKATERREKVERLVLCSGRIYHDIDAA
                     ASREEAEKVAVARVELLYPFPREQLAELFKAYPNLKEVVWVQEEPKNMGAWATMWRRL
                     PPIMPEGVNLEYIGRPERSSPSEGYSVAHIREQERIVLTALTP"
     gene            1551..>2950
                     /locus_tag="PLV77_03575"
     CDS             1551..>2950
                     /locus_tag="PLV77_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14958.1"
                     /translation="MPVSVSLEGQPYSASWPDSASFTARLKVEAGSQGADFGFQLDPT
                     GWGLEGVGFGSVFRDGQAVLDGPGELTSNPIGSGGPSGLPGYCSRGGQVPNFDYRLKL
                     LPDTSTVVEIPVSLNASPLEGMNSDLRIKYYASSPTGISPTIDAPVQVGGKTGVLVRF
                     DTGSSGQGPLTANNREPIRLSGSTAPSLPNTEILIKATPFAASPPAPPVRSISVTTDA
                     EGNFSREVVFDRSAFWRLTAHPMVDSSHDDEPSCGPTLVTTVDTYGDLPAEAGLSASA
                     PVSWPDQKDFEYRLSLTAGDHGADFNFEFQYSDWGLSGVSGTPFSIGEASLEGPGSLT
                     TVSTSVADPLPTACWRGGFGSVFNTYNLKLDPNQETVVTVPATLLAPRLAGMKPEIGA
                     VLSAGDGEPQELTAPVTITGEEGIRIIARVAGATGSVAALAPGLPFRLVGRTVPAVKN
                     RLIRLREAEQTTALQQ"
BASE COUNT          496 a         1015 c          915 g          524 t
ORIGIN      
        1 ccggccagga caccgaacgg ggcaccttct ctcagcggca cctggtgctg cacgacgaga
       61 agaccggcat caagtacgcg ccgatccagc acctgaagga tgcgaccgcg ccgttcgagc
      121 ttcacaacag ccccctctcg gagatcgcct gcctcggatt cgagtacggc tacgcctctt
      181 cgaaccccga ctcgctggtc atctgggaag cccagttcgg tgacttcgcc aactcggccc
      241 aggtcatcat cgacagcttc atcgcctcgg gcgaagcgaa gtggggcatg acctcacgcc
      301 tcacgatgct gctgccgcac ggccacgagg gagccggccc ggagcactcg agcgcccgca
      361 tggaacggtt cctccagctg gctgccgaag gatcgatgcg ggtcgccaat ccgtccaccg
      421 cggcgcagta cttccacctg ctcaggcgcc aggcgctgat caagaaggcc aggccactgg
      481 tcgtcttcac cccgaagggc ctgctccgcc agaccgcggc caccgccacg ctcgagcagc
      541 tgaccggcga gcacttccac ttcatcctcg acgaccccaa agccaccgaa cgccgggaaa
      601 aggtcgaaag gctggtgctc tgctcgggcc ggatctacca cgacatcgac gcggccgctt
      661 cccgcgagga agccgaaaag gtggccgtgg ccagggtcga gttgctctac cccttccccc
      721 gcgagcagct ggccgaactg ttcaaggcct acccgaacct gaaggaagtg gtctgggtcc
      781 aggaagaacc gaagaacatg ggcgcctggg cgacgatgtg gaggcgactg ccgccgatca
      841 tgcccgaggg cgtgaacctc gagtacatcg gccggccgga acgctccagc ccgtcggagg
      901 gctactcggt ggcccacatc cgcgagcagg aacgcatagt cctgaccgct ctcactccct
      961 gagccacctc ggccggggcc gcctcggtga cgatttgact ctccggggcg gcgggtttcc
     1021 gccgcattcc ggctaagacc ggactgcgta ttcgttcctc gttgacgcac atcgccaata
     1081 ggtgtataaa ctgcgttgca ggtccgcagt cgcagtaggg agaaaaggga aggagcagga
     1141 tcggaccagc gccccggagg ggttgccacc agtcgaaatt ttccaagccc gcttccggcg
     1201 gtttaccccc ggaagcgttc gcgtcggcat ccctccctgg ctaccgacgc gcaggcgtgg
     1261 tgagggtcgc gcggaccccc tgcctcccgc gaccctcgcc cgccactttt taggtggccg
     1321 ggccggtcta cgccggccct ttctttttga cccgccggcc cactcacatt cactgacggt
     1381 tgggccggct ccgctggttg gcaccactga ccagaccctg agctttgtcg ccagataccg
     1441 gcttcccgtt tatgctcttt tttgtcatga ggactttcag ggggtttttt gttttccttg
     1501 ccgctcttct cgcggccggt gtccttccgg ttgccgccca ggcgggaacg gtgcctgttt
     1561 ctgtttcgct tgagggtcag ccctactcgg ccagttggcc ggattcggct tccttcactg
     1621 cccggttgaa ggttgaagcc ggttcgcagg gagctgactt cgggtttcag ctcgacccga
     1681 ccggctgggg ccttgaaggc gtcggcttcg gaagcgtgtt ccgggacggg caggcggttc
     1741 tcgacggacc gggtgaactg acctcgaatc cgatcggctc cggtgggccc tccgggttgc
     1801 ccggatactg cagccggggc ggccaggtgc ccaacttcga ctaccgactg aaacttcttc
     1861 ccgatacctc gacggtggtc gagatcccgg tcagcctcaa tgcttcgccg ctggaaggca
     1921 tgaactcgga cctgcggatc aagtactacg ccagctcgcc gaccggcatt tccccgacga
     1981 tcgacgcgcc ggttcaggtc gggggcaaga ccggagtgct ggtcaggttt gacaccggga
     2041 gcagcggcca gggtccgctc acggcgaata accgggagcc gatccggctc agcggatcca
     2101 ccgccccgtc cctgccgaac accgaaatcc tgatcaaggc gacgcccttc gcggcatcgc
     2161 cgccagctcc gccggtccgc tcgatcagcg tcaccaccga cgcggagggc aacttcagca
     2221 gggaggtcgt cttcgaccgc agcgcgttct ggcgcctcac cgcccacccg atggttgact
     2281 cctcccacga cgatgaaccg agttgcggcc cgaccctcgt gaccacggtt gacacctacg
     2341 gtgacctgcc tgccgaggcc ggcctgagcg cttctgcccc ggtttcgtgg cccgaccaga
     2401 aggacttcga gtaccggcta tctctcactg ccggtgatca cggggctgat ttcaacttcg
     2461 agtttcaata ttcggactgg ggcctgagcg gggtaagcgg aactccgttt tcgatcgggg
     2521 aggcctcgct cgagggcccc ggttccctga ccaccgtttc gacatccgtc gccgatcctt
     2581 tgccgaccgc ctgctggcgg ggtggcttcg gcagcgtgtt caacacgtac aacctcaaac
     2641 tcgacccgaa ccaggagacc gtggtcaccg ttccggccac cctgctggct ccccgcctgg
     2701 ccggcatgaa gccggagatc ggggccgtgc tttcggccgg ggacggtgag ccgcaggaac
     2761 tgaccgcgcc ggtcacgatc accggcgagg aagggatccg cataatcgcc agggttgccg
     2821 gagcgaccgg gtccgtcgcc gcgctcgccc ccgggcttcc atttcgactg gtcggccgaa
     2881 ccgtgccggc cgtgaagaac cggctgatcc ggctgcgtga agcagaacag acaacggctc
     2941 tccaacagga
//
LOCUS       DAOQMF010000155         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19167_length_2944_cov_3.843544, whole genome shotgun sequence.
ACCESSION   DAOQMF010000155 DAOQMF010000000
VERSION     DAOQMF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19167_length_2944_cov_3.843544"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="PLV77_03580"
     CDS             <1..1621
                     /locus_tag="PLV77_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14959.1"
                     /translation="VAASLVFGVMMVSSPALALTPPPVDQASVVFSNGKRLIRIKADA
                     TDREVLTLKNSFVSSIDGLHDTALAGSPDGKSVLFRREDWSDRPTGPQGLMSLMVDGG
                     KPKLLVKQGSLSWIQAATFSADGSLIYFTKTRYAGDWESYTLSVNSIRPDGTGLETIW
                     SAPPVDLDDDEYSIGGITPAPDGRLLLTFDYDTSDSHLDLLDPDTGEVTPLKAGAAWG
                     SYSPDGSRIAFINMKQGYGALSVMNADGTGETRIAPKVPGFIDRGPDWSADGSRIIFQ
                     SVRDGWVASYGEEVWAVEPDGSCLTRLTNGAPESTAPVWLGTAGDSFEPGGCGDRAPN
                     AIVETSIPKRVRGLDPPRFWMGPEWEGMLYDDLIRIMNGVSFGYSDCTEFDYTQCSGY
                     REVSTDAVCSYPFFPENYAGMRRVRGTVIVRQNGDYRNEATVYTGGLSVDIQINHAKS
                     FADFVEAARLIQPVSGPRLHRLEGPVFTLGDVRTAHLVLRTKRQEGSFASLRRLGISP
                     REARGYLRFARDLDRIGKYGTTRCRGFSNLP"
     gene            1696..2295
                     /locus_tag="PLV77_03585"
     CDS             1696..2295
                     /locus_tag="PLV77_03585"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNC14960.1"
                     /translation="MAYEVPDLPYAYDALEPHIDEATMKVHHDKHHQAYVDKANAALE
                     GTEWADKPVEEVIANLSSLPADKQGPVRNNAGGHYNHSLFWEMLSPDGGGAPSGDLAA
                     AIDSAFGSFDDFKEQFKAGGIGQFGSGWVWLVKDGDGVAIVSTANQDSPLTDGKTPLL
                     GADVWEHAYYLKYQNKRPDYLDAFWNVVNWDYVAERFAS"
     gene            2467..>2944
                     /locus_tag="PLV77_03590"
     CDS             2467..>2944
                     /locus_tag="PLV77_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14961.1"
                     /translation="MIGLPTRTAVLPIPPKQLFFLALLAFAGLGMLISGCGSSESGDE
                     VARGAPSASDFPSAEGKTIEELLNDQTPSDKVVSPAAVVFEPGENRYPFGVFNVDRSQ
                     VQDADVAIYVAKSPQAPVEGPMPAEVVSLETKPAYRSKGAGGPGEANSFYLSHVKFD"
BASE COUNT          525 a          996 c          919 g          504 t
ORIGIN      
        1 cgtcgcggcg tcgctggttt tcggagtgat gatggtcagc tcgcctgccc tggcgctgac
       61 ccctccgccg gtcgaccagg cgtcggtggt cttctcgaac ggcaagcggc tgatccggat
      121 caaggccgac gccaccgacc gcgaagtcct gaccctgaag aactccttcg tcagcagcat
      181 cgacgggctt cacgacaccg ccctggccgg gtcgcccgac ggcaagtcgg tgctcttccg
      241 gcgggaagac tggtctgaca ggccaaccgg cccgcagggc ctgatgtcac tgatggttga
      301 cggcggcaag ccgaagctgc tggtcaaaca gggctccctg agctggattc aggccgcaac
      361 gttttcggcg gacggttcgc tgatctactt cacgaagacc aggtacgccg gggactggga
      421 gagctacacc ctgagcgtca actcgatccg tcccgacgga accggcctgg aaaccatctg
      481 gtccgcgccc ccggtcgatc ttgatgacga cgagtactcg atcggcggaa tcacgcccgc
      541 cccggacggc cggttgctcc tcaccttcga ttacgacacc agcgacagcc acctcgacct
      601 gcttgatccg gacaccggcg aggtgacgcc gctcaaggcc ggagccgcct ggggctcgta
      661 ttcgcccgac ggcagccgga tcgccttcat caatatgaag cagggctatg gcgcgctcag
      721 tgtgatgaac gctgacggca ccggcgagac gcggatcgcc ccgaaggtgc cgggcttcat
      781 cgatcgcggg cccgactggt cggccgacgg cagccggatc atctttcaaa gcgtgcgaga
      841 tggctgggtc gccagctacg gggaggaggt ctgggcggtc gaaccggacg gcagctgcct
      901 gacccggctg accaacggtg cgccggagag caccgcgccg gtctggctcg gcaccgcagg
      961 ggactccttc gaacctggcg gctgcgggga ccgggccccg aatgcgatcg tcgaaacgag
     1021 catccccaaa cgggtgcgcg ggctggaccc gccccggttc tggatgggcc cggaatggga
     1081 agggatgctc tacgacgacc tgatcaggat catgaacggc gtctccttcg gttactcgga
     1141 ctgcaccgag ttcgactaca cacaatgttc cggctaccgc gaagtcagca ccgacgcggt
     1201 ctgcagctat ccgttcttcc ccgagaacta cgccgggatg cgccgggtcc gcggaacggt
     1261 gatcgtcagg cagaacgggg actaccgcaa cgaggcaacc gtctacaccg gcgggctttc
     1321 ggttgacatc cagatcaatc acgcgaagtc cttcgccgat ttcgtcgagg cggcaaggtt
     1381 gatccagccg gtcagcggcc cgcggctcca ccgcctggag ggacccgtct tcaccctcgg
     1441 cgacgtccga acggcgcacc tggtcctccg cacgaaacgg caggagggtt cattcgcctc
     1501 gctgagaagg ctgggaatca gcccgcggga ggcccggggc tacctccgct tcgcccgaga
     1561 cctcgaccgg atcggcaaat acggcacaac ccgctgccgg ggattcagca acctgccgtg
     1621 aggcggagcg cgtcggaccg ttagggttct ggtacctgtc acgaaacctc aagaaaccag
     1681 tcaaggagtc ccctgatggc atacgaagtt cccgatcttc cctacgcata cgacgccctc
     1741 gagccgcaca tcgacgaggc gaccatgaag gtgcatcacg acaagcacca tcaggcgtat
     1801 gtcgacaagg ccaacgccgc cctcgaaggc accgaatggg ccgacaagcc ggtcgaggaa
     1861 gtcatcgcca atctttcctc gctgccggcc gacaagcagg gcccggtccg caacaacgcc
     1921 ggtggtcact acaaccactc gctcttctgg gagatgctct cgcccgacgg cggcggcgcc
     1981 ccgagcggcg accttgccgc ggcgatcgac tccgccttcg gttcctttga cgatttcaag
     2041 gagcagttca aggccggcgg catcggccag ttcggctccg gctgggtctg gctggtcaag
     2101 gacggcgacg gcgtcgccat cgtctcgacc gccaaccagg attctccgct gaccgacggc
     2161 aagaccccgc tgctcggcgc cgacgtctgg gagcacgcct actacctcaa gtaccagaac
     2221 aagcgcccgg attacctgga cgccttctgg aacgtagtca actgggacta cgtagcggag
     2281 cgcttcgcct cctagttatc ccaaccagag ccggcccatc cactcctgga tgtcgtgggc
     2341 cggcgggttt cactggcggc ccggcttagt ccgggccgct ttctttttgt gattctgtgg
     2401 gtgggttcac gttcttccca cccgcccaag cggggcgaag ctcgccgact acttatgatc
     2461 cttccgatga tcggccttcc cacccggacc gctgtcctgc ccatcccccc gaagcagctc
     2521 ttcttcctcg cgctgcttgc cttcgccggc ctcggcatgt tgatctccgg ctgcggcagc
     2581 agtgaatccg gagacgaggt tgcccgcggc gctccttcgg cttccgactt tccttcggcc
     2641 gaaggaaaga cgatcgaaga gctgctgaat gaccagaccc ccagcgacaa ggtcgtctct
     2701 ccggccgcgg tcgtgttcga accgggcgag aaccggtatc cgttcggtgt cttcaacgtg
     2761 gaccggagcc aggtccagga tgccgacgtg gcgatctatg tcgccaagtc tccgcaggcg
     2821 ccggtggaag gcccgatgcc ggccgaggtc gtttcactgg aaaccaagcc tgcctaccgc
     2881 tcgaagggtg ccggcggccc tggcgaggcc aacagcttct acctctcgca tgtgaagttc
     2941 gacc
//
LOCUS       DAOQMF010000156         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19209_length_2940_cov_3.805546, whole genome shotgun sequence.
ACCESSION   DAOQMF010000156 DAOQMF010000000
VERSION     DAOQMF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19209_length_2940_cov_3.805546"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="PLV77_03595"
     CDS             complement(<1..1527)
                     /locus_tag="PLV77_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNC14962.1"
                     /translation="MTEEQSEPEEREEPPAEEPVVEEPPAEEPVAGTPEPDPGEYADP
                     PTEELFAFEARVEEGEVPDLDPVFRAALLESSESALGEQPPSSEPPAGTGESAAAPEA
                     DQQPTTGEEQNTGEQPLTGEEPYTGEEPVTGTSEFEPPTDTGITAEQTAVLQATARSE
                     VHDAIEYSRRTGNAWDPPRPPTGGDFDKPAKRPRYWWRFLLATLLIVFSFATATQASV
                     MYFVNSIVEDLHKPNQKPLPPGILNADDGPQTIAIIGSDVRTGGGAASGDPGRSDTTL
                     LVRLDPDTNQIAMLSIPRDLYVDIPGYGYDKFNAAFSYGGTELTLKTLKSITGLDINH
                     YINVDFQGFADVIDAIHCVYVDVDRHYFNDNSQGGEEYATIDIEAGYQKLCGSDALDF
                     ARYRHTDSDLVRAARQQDLMGEVRNRLSFGELLSQRDELLKAFVDNTTTDIGETGDSK
                     DVFSLLKLLLDSRGAAVTEVTFPSTFTMRDGVSYVEATPDEIQAAVDKFLGFEDQPGP
                     V"
     gene            complement(1532..2680)
                     /locus_tag="PLV77_03600"
     CDS             complement(1532..2680)
                     /locus_tag="PLV77_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC14963.1"
                     /translation="MDELLALAEQLIGYDTSSEDGVHEAAGFIKGWLDSRGVETEQGV
                     CRGLPVITASHGDPADPTVILHGHIDVVPAGAGQFEARVEGDRLIGRGTYDMKAALAA
                     MMLTLPTEAEPLPGLRVVLGIVSDEESEEAENRGSNFLVREPDAAGPDRNGGTAATGG
                     MGLSGDFAITGEPTDMHVGVAAKGVLGLRLAVAGRAAHGSTPWLGENAVLLAVDMFRG
                     VESLPFARESSELFDRPSINLGRIWGGDALNKVPDSCAIDVDIRYLPHQDPAQIRDQI
                     AGLGPARMETIMELPPVSGGGTDSPWIQALTAAASRHHEGLSLSVGRDGASDAVSFIR
                     AGIPAVEFGPAGAGHHGPDEWVSIPSLIAYRSALKDFLKRAAKSAGRG"
BASE COUNT          485 a          954 c          973 g          528 t
ORIGIN      
        1 gaccggtccg ggctgatcct cgaaaccgag gaacttgtca accgcagcct gaatttcgtc
       61 cggggtggct tcgacatagg agacgccgtc ccgcatcgtg aacgtcgagg ggaaggtcac
      121 ctctgtcacg gcggcgcccc gtgaatcgag caggagcttc agcagcgaaa agacatcctt
      181 gctgtcaccg gtctcgccga tgtccgttgt cgtgttgtcg acgaaggcct tgagcagctc
      241 gtcgcgctgg ctcagcagct caccgaagct gagccggttg cgcacctcgc ccatcaggtc
      301 ctgctggcgg gccgcccgga ccaggtcgga gtcggtgtgc cggtaacggg cgaagtcgag
      361 cgcatcggag ccacaaagct tctggtagcc ggcctcgatg tcgatcgtcg cgtactcctc
      421 gccgccctgg ctgttgtcgt tgaagtaatg gcggtcaacg tcgacgtaga cgcaatggat
      481 cgcgtcgatc acgtcggcga atccctggaa gtcgacattg atgtagtggt tgatgtccag
      541 accggtgatc gacttgagcg tcttcagcgt cagctccgta ccgccgtagc tgaaggcggc
      601 attgaacttg tcgtacccgt atccggggat gtcgacatag aggtcgcggg ggatcgaaag
      661 catcgcgatc tgattggtgt cggggtcgag ccggaccagc agcgtcgtat cggaacggcc
      721 ggggtcgccg ctggccgcgc cgccgccggt gcggacgtcg gaaccgatga tcgcgatcgt
      781 ctggggcccg tcgtcagcgt tcagaatgcc gggcggaagt ggtttctgat ttggcttgtg
      841 gagatcctcg acgatgctgt tgacgaaata catcaccgac gcctgcgttg ccgtggcgaa
      901 ggaaaagacg atcaggaggg tcgccaggag gaagcgccac cagtagcggg ggcgcttggc
      961 cggcttgtcg aagtcacctc ccgtcggcgg ccggggcgga tcccaggcat tcccggtgcg
     1021 acgggagtat tcgatcgcgt catggacctc gctgcgagcc gtcgcctgga ggaccgcggt
     1081 ctgctcggcg gtgatcccgg tgtcggtggg cggttcgaac tcgctggtgc cggtcaccgg
     1141 ttcctcgccc gtgtacggct cttcgccggt cagcggctgc tcaccggtgt tttgctcctc
     1201 gccggtggtc ggttgctgat cggcctccgg ggcggctgcc gattccccgg tgccggccgg
     1261 cggttcggat gaaggcggct gttcgccgag cgcgctctcc gagctttcga gcagggccgc
     1321 ccggaagacc ggatcgagat cgggcacctc gccctcttcg acccgcgcct cgaaggcgaa
     1381 gagctcctcg gtcggagggt cggcatattc gcccgggtcc ggctcgggag tcccggcaac
     1441 cggctcttcg gctggcggct cttcgacaac cggttcttcc gctggcggct cttcccgctc
     1501 ttcgggttcg ctctgctcct cggtcatcgg atcaccccct cccggctgat ttcgcggcgc
     1561 gcttcagaaa gtccttcagg gccgaccggt aggcgatcag cgaggggatt gaaacccatt
     1621 cgtcggggcc gtggtgacca gctcccgccg ggccgaattc gacggccgga atcccggcgc
     1681 ggatgaacga aacggcatcg gaggctccat cccggccgac cgaaagggac aggccctcgt
     1741 gatgccttga ggccgctgcg gtcagagcct ggatccaggg cgagtcggta ccgccgccgc
     1801 tgaccggcgg cagttccatg atcgtctcca tccgggcagg gccgagcccc gcgatctgat
     1861 cgcgaatctg tgccgggtcc tggtgtggca ggtagcggat gtctacgtct atcgcgcaag
     1921 aatcgggaac cttgttgagc gcgtcgccgc cccaaatccg gccaagattt atcgaggggc
     1981 ggtcgaagag ctcggagctt tctctcgcaa agggtagtga ttcgaccccc cggaacatgt
     2041 ccacggcaag caaaacggcg ttttcgccca gccagggcgt cgaaccatgg gccgcccggc
     2101 cggccacggc caggcggaga ccgaggactc ccttggccgc cactccgaca tgcatgtcgg
     2161 tcggttcgcc ggtgatcgcg aagtccccgc tcaggcccat gccgccagtg gccgccgtcc
     2221 cgccgtttcg gtccgggccg gcggcatccg gctcacggac gagaaagttg ctgccacggt
     2281 tctcggcttc ttccgattcc tcgtccgaga cgatgccaag cacgacccgg agccccggca
     2341 acggctcggc ctcggtcggc agggtcagca tcatcgcggc gagcgcggcc ttcatgtcat
     2401 aggtgccgcg gccgatcagg cgatccccct cgacccgggc ttcaaactgc ccggctccgg
     2461 ccggaaccac atcaatgtga ccgtgaagga tcacggtcgg gtcggccgga tcgccatgcg
     2521 atgcagtgat caccggcagc ccgcgacaga ccccctgctc ggtctccacg ccccggctgt
     2581 cgagccagcc cttgatgaaa ccggcagcct cgtgcacccc gtcttccgaa gaggtgtcat
     2641 aaccgatcaa ctgttcggcc agggcgagca gttcgtccat cgaggaatca taagtcggcg
     2701 ggcttcaccc cgcctccccc gtcaacccgc catcccacag aagcaaccaa agtgtcgtgc
     2761 ctcctaatcg acataaggtg ccgcaaattc agccccgtga tggtgctgaa tatgcggcac
     2821 ctccgggcct gaaagtgcgg cacctccggg tctgaacggg caggcgagga gcgaagggtg
     2881 cggggccccg ggaaatcgag gagggagtta aggccacata acgcacccaa ctccgacttc
//
LOCUS       DAOQMF010000157         2936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19251_length_2936_cov_4.057619, whole genome shotgun sequence.
ACCESSION   DAOQMF010000157 DAOQMF010000000
VERSION     DAOQMF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19251_length_2936_cov_4.057619"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="arsM"
                     /locus_tag="PLV77_03605"
     CDS             <1..571
                     /gene="arsM"
                     /locus_tag="PLV77_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNC14964.1"
                     /translation="SASLGCGNPTALATLEPGEVVLDLGSGGGIDVLLSARRVGPTGK
                     AFGLDMTDEMLELARSNQKEAGVENVEFLKGEIEAVPLPDDSVDVVISNCVINLSGDK
                     ARVLREAARVLRPGGSFAVSDVIADPDMTEETRADMREWTGCIAGALTREEFESNLAA
                     AGFEDIQIEATHRVHDQAESAIIRATLPA"
     gene            568..942
                     /locus_tag="PLV77_03610"
     CDS             568..942
                     /locus_tag="PLV77_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14965.1"
                     /translation="MSTDEIQLNDPQTLYERWEGAQWNPFTVPLEWDIEQWAGMGDRD
                     RGLVYWVLSSLMVAEERITTKFSGLVGAHGSEEEASFLATQQVDEARHIGYGVWFLRE
                     SVRESPDVAPEAIRSMLRTLLP"
     gene            943..1092
                     /locus_tag="PLV77_03615"
     CDS             943..1092
                     /locus_tag="PLV77_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14966.1"
                     /translation="MAESLSPSPGDDGPDLDALGVSGEDIRNFALGGLTRRLEIIGVP
                     LETVF"
     gene            1174..2346
                     /gene="chrA"
                     /locus_tag="PLV77_03620"
     CDS             1174..2346
                     /gene="chrA"
                     /locus_tag="PLV77_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNC14967.1"
                     /translation="MPVDQDSRAAIFWRFLKFGLLAWGGPAAQIEMIRHECVEQEKWI
                     SAETFKKQLAVYQILPGPEAHELCVYLGRVRGGKLGGFLAGLGFMLPGFILMLGLSVL
                     YVKASLAGHLDQVFYGLTAAVGALVARAVVRLSKNFLTDIPLVIIAALAFGLTLGISA
                     SFVLVLLGAGIAYELWTNRHGWTRRTNSFGFSPLVLVLAATGAITLSLTAEIFFEGLK
                     AGLLTFGGAFTVIPYLQESAVNNHHWLTDSQFVDGLAMSGVLPAPLIIFSTFVGYIAG
                     GMTGALVMTLGIFLPAFIFPIFFHRQLVAVAENARLHPFLLGVAAGVIGLIAAVTVSI
                     LQTSVVDVWTGLIALGAFLALQKWHSKLTVVYVMAAAGLIGVPLQGNRRLSSDRDP"
     gene            complement(2329..>2936)
                     /locus_tag="PLV77_03625"
     CDS             complement(2329..>2936)
                     /locus_tag="PLV77_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNC14968.1"
                     /translation="IGLIILTVILTWDRGYRATAAKRAELEGSLTGLVDEINRKDPGP
                     IRVRGTAVYLNARTNTAPLAFRSALERDGIVHDHVVVLSLVTARQPYVEDKDRIVIDD
                     LGYDYDGISHITATVGFMDVPDVPYLLELANPQVPDGPLDFSKAYYYLSNVLIRPYGD
                     TRLHRLLKHLYITMARNASSPVDHFRLPAERTVIESSGISI"
BASE COUNT          489 a          919 c          972 g          556 t
ORIGIN      
        1 gagcgcgtcc ctcggctgcg gcaacccgac cgccctcgcc accctcgagc cgggcgaggt
       61 cgtcctcgac ctcgggtccg gtggcggcat cgacgtcctg ctctcggccc ggcgggtcgg
      121 cccgaccggc aaggccttcg gcctcgacat gaccgacgag atgctcgaac tcgctcgcag
      181 caaccagaag gaagccggtg tcgagaacgt cgaattcctg aagggggaga tcgaagcggt
      241 acccctcccc gacgactcgg ttgacgtggt gatctccaac tgcgtgatca acctttccgg
      301 cgacaaagcc agggtcctga gggaggcggc gcgggtcctt cgaccgggcg ggagttttgc
      361 cgtctcggac gtgatcgccg atccggacat gaccgaagag acacgggcgg acatgaggga
      421 atggaccggc tgcatagccg gcgccctgac ccgcgaagag ttcgaatcga accttgcggc
      481 agccggcttc gaggacatcc agatcgaggc gacacaccgc gtccacgacc aggccgaatc
      541 ggcgatcatc cgagctaccc tgcccgcatg agcaccgacg agatccagct gaacgatccc
      601 cagaccctct acgaaaggtg ggaaggggcg cagtggaatc cgttcacggt accgctggag
      661 tgggacatcg agcagtgggc cggaatgggg gacagggatc gaggactggt ttattgggtg
      721 ctctcgtcac tgatggtcgc cgaggagaga atcacgacca agttctccgg tctggtcggg
      781 gcccacggat cggaagagga agcgagcttt ctcgcgaccc agcaggtcga cgaggcccgc
      841 cacatcggct acggagtctg gttccttcgc gagtcggtcc gggagtcacc ggatgtggcg
      901 cctgaggcca tccggtcgat gcttcggacg ctgctgcctt aggtggccga atcgctctcg
      961 ccgtctcccg gagatgatgg cccggacctc gacgctctcg gtgtcagtgg cgaggacatc
     1021 cggaacttcg ccctgggcgg cctgaccagg cgcctggaga taatcggcgt tccactcgag
     1081 acggtcttct agaactgcgg gtggtcaccg gcgtctgcgc gctttctctc cgggaagtaa
     1141 cccggatgcg gccggaactt ggtaaaagct gccatgcccg ttgaccagga ttcccgagca
     1201 gccatcttct ggcgcttcct caagttcggt ttgctcgcct ggggaggacc ggcggcccag
     1261 atcgaaatga tcaggcacga gtgcgtcgag caggagaagt ggatctccgc ggagaccttc
     1321 aagaagcagc tcgctgtcta ccagatactt cccggaccgg aagcccatga gctctgtgtc
     1381 tacctgggcc gggtgcgggg cgggaagctc ggcggattcc tcgccggtct cggcttcatg
     1441 ctgcccggct tcatcctcat gttggggctc tcggtcctct acgtcaaggc cagcctggcc
     1501 gggcatctgg accaggtctt ctacggactg acggcagcgg tcggcgccct ggtggcccgc
     1561 gccgtggtcc ggctctcgaa gaacttcctc accgacattc cgttggtcat catcgctgcg
     1621 ctcgccttcg gactgaccct gggcattagc gcaagcttcg tgctcgtcct gctcggcgcc
     1681 ggcatcgcgt acgagctgtg gaccaaccgg catggctgga cccggcggac caattcgttc
     1741 gggttcagtc ccctggtgct cgtgctggcg gcgaccgggg cgatcaccct ttccctgacg
     1801 gccgagatct tcttcgaagg cctgaaggcc gggctgctga ccttcggcgg ggccttcacg
     1861 gtcattccct acctccagga aagcgccgtc aacaaccatc actggctgac tgacagccag
     1921 tttgtcgacg gactggcgat gagtggtgtc ctgccggcac cgttgatcat tttctccacc
     1981 ttcgtcggat atatcgccgg cggaatgacc ggcgccctgg tcatgaccct gggcatcttc
     2041 ctgcccgcct tcatcttccc gatcttcttt caccgccagc tcgtcgcggt cgccgaaaat
     2101 gcccggcttc atccgttcct gctgggagtc gctgcggggg ttatcgggct gatcgccgcg
     2161 gtcacggtct cgatcctcca gaccagcgtc gtcgatgtct ggaccgggct gatcgccctc
     2221 ggggctttcc tcgccctcca gaagtggcac tcgaagctga cagtcgtcta tgtaatggcc
     2281 gccgccgggc tgatcggagt cccgctccaa ggcaaccgtc gtctgagctc agatcgagat
     2341 ccctgaactt tcgatcacgg ttctttcggc ggggaggcgg aagtggtcta ccgggcttga
     2401 ggcgtttcgg gccatcgtta tgtagaggtg cttgaggagg cggtggagcc tggtgtcacc
     2461 gtacgggcgg atcagaacgt tggagaggta gtagtaggcc ttgctgaaat cgagcgggcc
     2521 gtcggggacc tgggggttgg cgagctccag cagataggga acgtcgggaa catccatgaa
     2581 accgaccgtg gcggtgatgt gggagatccc gtcgtagtcg tagccgaggt catcgatcac
     2641 gatccggtcc ttgtcctcga catagggctg gcgggcggtg accagggaga gcaccaccac
     2701 gtggtcgtgg acgatcccgt cgcgctcgag ggccgagcgg aaggccagcg gagcggtgtt
     2761 ggtccgggcg ttcaggtaga cggcggtgcc ccggacccgg atcggccccg gatccttgcg
     2821 gttgatctcg tccaccagtc cggtgagcga accttccagc tcggcccgct ttgccgcggt
     2881 cgcccggtaa ccgcgatccc aggtcaggat cacggtcagg atgatcaggc cgatcg
//
LOCUS       DAOQMF010000158         2936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19254_length_2936_cov_3.706699, whole genome shotgun sequence.
ACCESSION   DAOQMF010000158 DAOQMF010000000
VERSION     DAOQMF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19254_length_2936_cov_3.706699"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PLV77_03630"
     CDS             <1..1638
                     /locus_tag="PLV77_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC14969.1"
                     /translation="EAERVIGYLGFFTASYLTMRTPKQREWFARGIAAGLAVIVLLAL
                     GDRLLPGGEPGVVPGIDRLSFPLGYWNANGVCAATALVLFTWFAAVTRSKGWRAGSVA
                     MAMLSGTALYLTYSRGGLLLAVIAAILLCLLTPRRLRVLATLAVVVAGTVPILLTVNL
                     YPEISGDSFGDPSLGESLIVILVALGASLLAAAVLEGLFGLGRRQPEVTERAREISRD
                     RRVLIGIIGAGATLLIVFSFAFGQNVWDQFTDDDVPVPTDARSRFTELSGSWRYEFNR
                     VALDTFSDNPVLGTGAGTYPIEWSRRREVPVITQDAHSFYLENLAELGIPGGLISIAL
                     VISLLTLAAWAFRRRLGREAPVILALTAGLLFSLAFDWFWKLGVTAALLMCLAAWIAS
                     AEAVDPERPQTGAGTGFKVAGLLAAWVSIVILAVPALADHYVQASADSVRAGNVDRAY
                     DQAKAASRLEPFSADPHMQLGTIAEGRGQYALALAEYNKAIELEPENWHALVLRFGLN
                     YHAGKLQAARADYARLREINPLYFSEYSFREIQDMAS"
     gene            1772..>2936
                     /locus_tag="PLV77_03635"
     CDS             1772..>2936
                     /locus_tag="PLV77_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14970.1"
                     /translation="MLLAALLISFLGLTTFVSKADAARSFQTGIFDGSVYDHGDDEAF
                     DRTADSGATLIRSNLYWTYMVANGDSADRPGSEGQPFNATDPGSPYYNWGVYDRMVRK
                     SAARGLQMVFSIVSPPRWARISSCADSGVCSPRPADYADFATAAARRYDGTFDPGDGN
                     GVLPRVRFWQAANEPNFYLFYKPLLKPNGAPAAPYEYRKILNAFYGAIHAVDNSNVVI
                     AAGLAPNGKKGKSISPLDFTRRALCMTGSYKHPRPIAGCKFKVKADAWAVHPYTTGGP
                     THVPANGNDMSVAALPRLQKLLKAATRAGRLSGAGGHTQLWVTEFSWDSKAPDPGGVP
                     WALQTRWVAQAMYLMYKANVSTMLWFGLRDQPKGANWSYTFQSGLYLRGATIAK"
BASE COUNT          470 a         1043 c          889 g          534 t
ORIGIN      
        1 gaagccgaga gagtcattgg ctatctcggt ttctttacag ccagttacct caccatgaga
       61 accccgaaac agcgcgaatg gttcgcccgg ggcatcgcgg ccggcctggc ggtgatcgtc
      121 ctgctggcgc tcggcgaccg gttgctgccc ggaggggagc ccggagtcgt tcccggaatc
      181 gaccgtctca gcttcccgct cggctactgg aacgcgaacg gcgtctgcgc cgccacggcc
      241 cttgtcctct tcacctggtt tgccgcggtg accaggtcga aagggtggcg ggccggatcc
      301 gtggccatgg ccatgctctc cggaaccgcg ctctatctca cctactcccg gggcggcctg
      361 ctgctcgccg tgatcgcggc catcctgctt tgcctgctca ccccgcgccg gctccgggtc
      421 ctggccaccc tggcggtcgt ggtcgccgga acggtcccga tcctgctcac ggtcaatctc
      481 tatccggaga tctccggcga cagcttcggt gacccctccc tcggagagag cctgatcgtg
      541 atccttgtcg ccctcggggc ctcactgctc gcggcagcag tcctcgaagg cctgttcggg
      601 ctcggccgcc gtcagccgga ggtgacagag cgagcccgcg agatttcccg ggatcgaagg
      661 gtgctcatcg gcatcatcgg ggccggagcc accctgctga tcgtcttttc gttcgcgttc
      721 ggccagaacg tctgggatca gttcaccgac gatgacgttc ccgtcccgac cgatgcccgc
      781 tcacgcttca cggaactttc gggatcctgg cgatacgagt tcaaccgggt tgccctggac
      841 accttctccg acaacccggt tctcggaacc ggtgccggca cctacccgat cgaatggtcc
      901 cgccgccgcg aggtgccggt gatcacccag gacgcccact ccttctatct agagaacctg
      961 gccgagctcg gcatcccggg cgggctgatc tcgatcgccc tggtcatttc actgctgacc
     1021 ctggcggcct gggcttttcg ccggcgtctg ggaagggaag ctccggtgat cctcgccctg
     1081 actgccggac tgctcttctc cctggccttc gactggttct ggaaactcgg ggtcaccgcg
     1141 gccctgctga tgtgcctcgc cgcctggatc gcctcggccg aggcggtgga cccggagcgc
     1201 ccgcagaccg gtgccggtac cgggttcaag gtcgccggcc tgctcgccgc gtgggtctcg
     1261 atcgtgatcc tggccgtccc ggcactggcc gaccactacg ttcaggcaag cgccgattcg
     1321 gttcgggccg gcaacgtcga ccgggcatac gaccaggcga aagccgcctc aaggctggaa
     1381 cccttctcgg ccgaccccca catgcagctg ggcacgatcg ccgaagggcg gggccagtat
     1441 gccctggcgc ttgccgagta caacaaggcg atcgaacttg agccggagaa ctggcatgcc
     1501 ctggtcctgc ggttcggact caactaccac gcagggaagc tgcaggcggc ccgggccgac
     1561 tacgcgcgcc tgcgagaaat caatccgctc tacttctccg agtactcatt cagggaaatc
     1621 caggacatgg cctcctgacc cccaactcgc cggttcgcta ctttctgcct ttggcgatgt
     1681 tcaagtacac aagcgccacc cgagacttcg cgccctcgag cgaagtttga ccctccgaaa
     1741 ggatcccttg cacgcaatct cgaatcgcgg aatgctgctc gcagcactgc tgatcagctt
     1801 tctcggcctg accacctttg tctcaaaggc cgacgcagcg cgctctttcc agaccgggat
     1861 ctttgatggc agcgtgtatg accacggtga cgacgaggcc ttcgatcgca ccgccgacag
     1921 cggcgcaacg ctgatcagga gcaaccttta ctggacctac atggtcgcca acggcgattc
     1981 ggccgatcgc ccgggatccg agggccagcc tttcaacgcg accgaccccg gcagccccta
     2041 ctacaactgg ggggtctatg accgcatggt tcgcaaatct gccgcccgcg ggctccagat
     2101 ggtcttctcg atcgtcagcc ctccgcgctg ggcacggatt tcgtcctgtg ccgattccgg
     2161 tgtctgctcg ccccggcccg ccgattacgc ggatttcgcg accgcggctg ccaggcgcta
     2221 cgacggaacc ttcgaccccg gtgacggcaa cggtgtcctg ccccgcgtga ggttctggca
     2281 ggccgccaac gagcccaact tctacctctt ctacaagccg ctgctgaagc ccaacggggc
     2341 accggccgct ccctacgagt accgcaagat cctcaacgcc ttctacggcg cgatccacgc
     2401 ggttgacaac agcaacgtcg tgatcgctgc cgggctcgcc ccgaacggca agaagggcaa
     2461 gtcgatctcg ccgctcgatt tcacccgtcg cgccctctgc atgaccggct cctacaagca
     2521 tccccgcccg atcgccggct gcaagttcaa ggtgaaggcc gacgcctggg cggttcaccc
     2581 gtacacgacc ggcggtccga cccacgttcc cgccaacggc aacgacatgt cggttgccgc
     2641 cctgccccgc ctgcagaagc tgctcaaggc ggcgaccagg gccggcaggc tctccggcgc
     2701 gggcggccac acgcagctgt gggtcaccga gttcagctgg gattcaaagg cgccggaccc
     2761 gggcggagtg ccgtgggccc tgcagacccg ctgggtggcg caggccatgt acctgatgta
     2821 caaggcgaac gtttccacca tgctctggtt cgggctccgc gatcagccca agggtgcgaa
     2881 ctggtcctac acgttccagt cgggccttta cctgcgcggc gcgacgatcg ccaagg
//
LOCUS       DAOQMF010000159         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19332_length_2930_cov_2.933217, whole genome shotgun sequence.
ACCESSION   DAOQMF010000159 DAOQMF010000000
VERSION     DAOQMF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19332_length_2930_cov_2.933217"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLV77_03640"
     CDS             complement(<1..1316)
                     /locus_tag="PLV77_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14971.1"
                     /translation="MLTLLAVANGARAEILKTEVLPGGVQRTTYRIGPVDVTPGQNRI
                     AYRPIEGASRPAVDGWITRIKPDLVYEDGSIPDSSKVMFHHGVWINLSTNEQFYATGE
                     EKTILDLPEGFGYRYKTDEGWLLNDMIHNLTPQAMKLYFEYTLDFIPDTAAEAAGIVR
                     ARPIWMDVQNGSIYPVFDVWRDSGGDDGEFTYPEDATNPYPPGNQKNVKSIPANGMLI
                     ATTGHVHTGGLSTDLYLRREGATYQGPNCPERISYEDELKPLQQKNDSLVARINKLKQ
                     KNAKMTRTQQKIRRSMKNRQSRSKAKSLIKDIKRRKKSNSKTIAMVNRLRAKNKIQLD
                     KVKATDKAERERDAACRGTQPSVDDGNRVHLFKSTADYFDPRGPISWDMAMVSTDNDW
                     RVRVKAGDKLELQTTYETKRASWPESMGINIVYWVRDSAYAAAANAP"
     gene            complement(1500..>2930)
                     /locus_tag="PLV77_03645"
     CDS             complement(1500..>2930)
                     /locus_tag="PLV77_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNC14972.1"
                     /translation="IDPGSFVEYGRYAIAAQRARFDTDDLISRTPGDGLIAGTATVNG
                     ELFGENSRCAVLSYDYTVLAGTQGAVGHLKKDRLFELIEKMKLPTIFFAEGGGGRPGD
                     TDFPVVSALQVRAFALWAGLSGLVPRVAIVKGRCFAGNAVIAGCADLIVATKDSSLGM
                     GGPAMIAGGGLGEVAPDEVGPVSFQEPNGVLDLVVADEAEATAAAKKLIGYFQGRSPW
                     TDGGESDQAPLREMVPERERRAFDVRPVIETIADPDSVTLLREKFAPELVTALARIEG
                     RPVGVIANDSRHMAGTITSDSADKAARLLQLCESFGLPVISLVDTPGFMVGPAHEQTA
                     LVRHTSRLLVAGAALTVPLISVILRRAYGLGAQAMVGGSLHEPLLTVAWPSAHLGPMG
                     LEGAVRLAMRKDLEAIADEEEREKRVRELTALAEQNARAMNAAQLFEIDDVIDPAETR
                     SVIASTLAAAPERVSERAPHRFVDTW"
BASE COUNT          452 a          952 c          987 g          539 t
ORIGIN      
        1 ggagcgttgg ccgcggcggc gtaggcggaa tcacggaccc agtacacaat gttgatgccc
       61 atcgactcgg gccaggaggc ccgcttcgtc tcgtaggtcg tctgcaactc cagcttgtcc
      121 ccggccttga cccggacccg ccagtcgttg tcggtgctga ccatcgccat gtcccaggag
      181 atcggaccgc gcgggtcgaa atagtccgcg gttgacttga acaggtggac gcggttgccg
      241 tcgtcgaccg acggctgggt accgcggcag gcggcgtcgc gctcgcgctc ggccttgtcc
      301 gtggccttga ccttgtcgag ctggatcttg ttcttcgccc ggaggcggtt gaccatcgcg
      361 atggtcttcg agttggactt cttgcgccgt ttgatgtcct tgatcaggct cttcgctttc
      421 gacctgctct gccggttctt catcgagcgc cggatcttct gctgggtccg cgtcatcttc
      481 gcgttcttct gcttgagctt gttgatccgc gccaccaagc tgtcgttctt ctgctgaagc
      541 ggcttcagct cgtcctcgta ggagatccgt tccgggcagt tcggcccctg gtaggtggcg
      601 ccctcgcggc gcaggtacag gtcggtcgag agtccgccgg tgtggacgtg gcccgtggtc
      661 gcgatcagca tgccgtttgc cgggatcgac ttgacgttct tctggttgcc gggcgggtag
      721 gggttggtcg cgtcctcggg ataagtgaac tcgccatcgt ccccgccgct gtcacgccag
      781 acgtcgaaga cggggtagat gctgccgttc tggacgtcca tccagatcgg acgggcccgc
      841 acgatgccgg cggcttcagc cgcagtgtcc gggatgaagt cgagcgtgta ctcgaagtag
      901 agcttcatcg cctgcggggt gaggttgtgg atcatgtcgt tcagcaacca gccctcgtcc
      961 gtcttgtagc ggtaaccgaa gccctcgggc aggtcgagga tggtcttttc ttcgccggtc
     1021 gcgtagaact gctcgttggt cgagaggttg atccagaccc cgtgatggaa catcaccttg
     1081 ctcgagtcgg gaatcgagcc gtcctcgtag acgaggtccg gcttgatccg ggtgatccag
     1141 ccgtcgacgg ctggccggga ggcaccttcg atcgggcgat aggcaatccg gttctgaccc
     1201 ggtgtcacgt cgaccgggcc gatccgatag gtggtcctct gaaccccgcc gggcagcacc
     1261 tcggtcttga ggatctcggc gcgggcgcca ttggcgaccg cgagcaaagt caacactgct
     1321 gcaacgaaaa ctcatgccgc gatgaaccag cggcccggca cagcagacga ttcggctttc
     1381 ccgatttcca tcaaccctct cccttgactg cgtaaaaaga caacttcaag ttaccccata
     1441 accgcagagg gacctccggg ttgcgggtct ggccgaaaga gaccggcccg ccgccggctt
     1501 caccaggtgt caacgaagcg gtgcggcgca cgctctgaga cccgttcggg cgcggcggcc
     1561 agagtcgagg cgatgaccga tctggtctcg gccggatcga tcacgtcatc gatctcgaag
     1621 agctgggccg cgttcatggc ccgcgcgttt tgctcggcca gggcggtcag ctcgcgaacc
     1681 cgcttttccc gctcctcctc gtcggcgatc gcctcgaggt ccttgcgcat cgccaggcgt
     1741 acggcaccct cgagacccat cggcccgagg tgggcgctcg gccaggcgac ggtgagcagg
     1801 ggttcgtgga ggctgccgcc gaccatcgcc tgcgcgccca gcccgtaggc ccggcgcagg
     1861 atcaccgaga tcagcggaac cgtcagggcg gcgcctgcaa ccagcaggcg ggaggtgtgc
     1921 cggaccaggg cggtctgctc gtgggccggc ccgaccatga agccgggagt gtcaaccagg
     1981 gagatgacgg gcaggccgaa gctctcgcaa agctgaagca agcgggccgc cttgtcggcc
     2041 gagtcgctgg tgatcgtgcc ggccatgtgg cgggagtcgt tggcgatcac gccgaccggc
     2101 cggccctcga tccgggccag cgcggtgacc agttccggag cgaacttctc gcgcagcagg
     2161 gtgaccgaat ccggatcggc aatggtctcg atcaccgggc gcacgtcgaa agcccgtcgc
     2221 tcgcgttctg ggaccatctc gcggagcgga gcctggtcgc tttccccgcc gtcggtccag
     2281 gggctgcggc cctggaagta accgatcagc ttcttcgccg ccgcggtcgc ctccgcttca
     2341 tcggcgacga cgaggtcaag cacaccgttg ggctcctgga acgaaaccgg accgacctca
     2401 tccggggcga cctcgcccag acccccgccg gcgatcatcg ccgggccgcc catgccgagc
     2461 gaggagtctt tggtggcgac gatcaggtcg gcgcagccgg cgatcaccgc attgccggcg
     2521 aagcagcggc ccttgacgat cgccaccctc gggaccaggc ccgagagccc ggcccaaagt
     2581 gcgaaggccc tcacctgcag ggccgagacg accgggaaat cggtgtcgcc ggggcggccg
     2641 ccaccaccct cggcgaagaa gatcgtcggc agcttcatct tctcgatcag ctcgaagagc
     2701 cggtccttct tgaggtggcc gaccgctccc tgggtgccgg ccagcaccgt gtagtcgtag
     2761 gagagtacgg cgcatcgaga gttctcgccg aagagttcgc cgttgaccgt ggcggtgccg
     2821 gcgatcagtc cgtcgccggg cgtgcgactg atcaggtcat cggtgtcgaa gcgggcccgc
     2881 tgggcggcga tcgcgtaccg gccgtactcg acgaagctgc cggggtcgat
//
LOCUS       DAOQMF010000160         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19397_length_2924_cov_2.881143, whole genome shotgun sequence.
ACCESSION   DAOQMF010000160 DAOQMF010000000
VERSION     DAOQMF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19397_length_2924_cov_2.881143"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLV77_03650"
     CDS             complement(<1..387)
                     /locus_tag="PLV77_03650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC14973.1"
                     /translation="MESSKTNSLAVLIAARNEADVIAATIGSLREAFPGAVLWVADDA
                     SDDGTADAAMAAGAKVVSRGKPHGKGGNMTACAEAMLSDPGRDGEGPPDCVLLCDGDL
                     GASAGHLAPLVDAVEAGECDLAVAMFA"
     gene            455..667
                     /locus_tag="PLV77_03655"
     CDS             455..667
                     /locus_tag="PLV77_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNC14974.1"
                     /translation="MANIEAHITGNVWKIQVKVGDEVSEEDEVIILESMKMEIPVESE
                     DDGVVKEILCEEGQSVTEGDVLIVLE"
     gene            692..1513
                     /locus_tag="PLV77_03660"
     CDS             692..1513
                     /locus_tag="PLV77_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC14975.1"
                     /translation="MADVTSIETGTEKVRLDFPAEAVARLKISNPPKRNALDHEILDA
                     ITATVPRLDQGIEVRCLVITGEDPVFSAGYDIGGIPAESFETDAEALVAHPFTAALDA
                     IEDFPWPTVAALNGHCLGGALEMAVTCDLRIAAPHAKLGMPPAKLGLIYGHTGLIRFI
                     RTIGLPRTRELFFTGDMLPAGRADEIGLVNRVTAGDEDFAVEAVEFAAGIAANAPLSM
                     KGNKQAIGTITENPVLSEDQVRELVELRESCFTSEDFLEGITAFGEKRKPNWTGR"
     gene            complement(1578..2780)
                     /locus_tag="PLV77_03665"
     CDS             complement(1578..2780)
                     /locus_tag="PLV77_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNC14976.1"
                     /translation="MASALRKSPLRAAIGIALLLAGVAALLLLSSPGGDSGPSSGTTT
                     NSAGAGPFVKSKSASSPFPPLTRNLSAGLTNHQMAGQRIVAGFEGTTVPPVIRRRVKA
                     GLIGGVILFADNLGSDARIRHLTRALQRIRRPDGLRRYPLMIMVDQEGGLVKRLPGAP
                     NASAEQMGRRGPAFSRRQGALAGLNLKTSGFNVDLAPVLDVARPGGVIADTDRGFGST
                     VKKVKATAIPFARALQQTRVAATAKHFPGLGAARQNTDFAVQKIRLSRAALRRVDMAP
                     YEPFIAARGKLIMVGSAIYPALGRRPAMFEPKIVRGELRQRLGFQGVTITDAMGTVAV
                     NDFGGTKRAAISAAHAGMDILLYGDWQSAGRAEQAIAVRLRTGKLPRGQFTRAVNRIL
                     QLRSSLAD"
BASE COUNT          507 a          968 c          993 g          456 t
ORIGIN      
        1 ggcgaacatg gcgaccgcga ggtcgcattc gcccgcctcg accgcgtcga ccagcggggc
       61 gaggtggccg gccgaagcgc ccaggtcccc atcgcagagc aggacgcaat ccggcgggcc
      121 ctcgccatcc cggccggggt cgctgagcat cgcctcggcg caggcggtca tgttgccgcc
      181 cttgccgtgg ggcttgccgc ggcttacgac tttcgctccg gcggccatcg ccgcgtcggc
      241 ggtgccgtca tcggaggcgt cgtcggccac ccagagcacg gcgccgggaa aggcttcgcg
      301 gagagagccg atcgtggcgg cgatcacgtc ggcctcgttt cgtgccgcga tcaggaccgc
      361 cagtgagttg gtcttcgaac tctccaccgt gggcggactc tagaggttga ggccgcgacc
      421 tctgtggatg gccgtggcga taaagttccg gctcatggcg aacatcgaag ctcacatcac
      481 cgggaacgtc tggaagatcc aggtcaaggt cggggacgaa gtctccgaag aagacgaagt
      541 gatcatcctc gagtcgatga agatggagat cccggtcgag tccgaggacg acggcgtggt
      601 caaggagatc ctctgtgaag agggccagtc cgtgaccgaa ggcgacgtcc tgatcgtcct
      661 cgagtagccg tcccccggcg agccgacgcc gatggctgac gtgacctcga tcgaaaccgg
      721 cacggagaag gtccggcttg acttccccgc ggaagcggtc gcccgcctga agatttcgaa
      781 cccgcccaag cggaacgcgc tcgaccacga gattctcgac gcgatcacgg ccacggtgcc
      841 ccggctcgat caaggcatcg aggtccgttg cctggtcatc accggcgagg acccggtctt
      901 ctcggcgggg tatgacatcg gcggcatccc ggccgagagc ttcgaaaccg acgccgaggc
      961 tctcgtcgcc cacccattca ccgccgccct cgacgcgatc gaggactttc cctggccgac
     1021 cgtcgccgcc ctgaacggtc actgcctcgg aggagcgctg gagatggcgg tcacctgtga
     1081 cctgaggatc gccgccccgc acgcgaagct cggcatgccg ccggccaagc tcggtctgat
     1141 ctacggccac accggcctga tccgcttcat ccgcaccatc ggcctgcccc gcacccgcga
     1201 gcttttcttc accggtgaca tgctgcccgc cggccgggcc gacgaaatcg gtctggtcaa
     1261 ccgggttacc gccggggacg aggatttcgc ggtcgaggcg gtcgaattcg cggccggcat
     1321 cgccgccaac gccccccttt cgatgaaggg caacaagcag gcgatcggaa cgatcaccga
     1381 aaacccggtc ctctccgaag atcaggtgag agaactggtc gaactccgcg agtcctgctt
     1441 tacctccgaa gacttcctcg agggcatcac cgccttcggt gagaagcgca agccgaactg
     1501 gaccggccgc tagccgcgac caggacccac ggttttccac aatatttgtg ggaaaccgtg
     1561 gacttcgaga cgaggcttca gtcagcgagg ctggagcgaa gctgaaggat ccggtttacc
     1621 gcgcgggtga actggccgcg gggcagtttg ccggtgcgca ggcgaactgc gatcgcctgt
     1681 tcggccctgc cggctgattg ccagtcgccg tagaggagga tgtccatgcc ggcgtgggcg
     1741 gcgctgatcg cggcacgctt ggttccgccg aagtcgttga ccgcgaccgt gcccatcgca
     1801 tcggtgatcg tcacgccctg aaagccgagg cgctggcgga gctcgccgcg gacgatcttc
     1861 ggttcgaaca tcgccggccg gcggcccagc gccgggtaga tcgcagagcc aaccatgatc
     1921 agcttgcccc gggcggcgat gaacggctcg taaggggcca tgtccacccg gcgaagtgcc
     1981 gccctcgaga gccggatctt ctggaccgcg aagtcggtgt tctggcgggc cgcacccaga
     2041 cccgggaaat gcttggcggt ggcggcgacc ctggtctgct gaagagcccg ggcgaagggg
     2101 atcgcggtcg ccttcacctt cttcacggtt gagccgaaac cgcggtcggt gtcggcgatg
     2161 acccctccgg gacgggccac gtccagcacc ggagcgaggt cgacgttgaa gcccgacgtc
     2221 ttgagattga ggccggcaag agctccctgg cgacgggaga aggcggggcc ccgcctgccc
     2281 atctgctcgg ccgaggcatt cggggccccc ggcagccgtt tgacgagccc gccttcctgg
     2341 tcgaccatga tcatcagcgg gtagcggcgc aggccgtcgg gccgccggat ccgctgaagc
     2401 gccctggtca gatgccgaat ccgcgcgtcc gagccgaggt tgtcggcgaa gaggatcact
     2461 cccccgataa gtccggcctt gacccggcgg cggatgaccg gagggaccgt ggtgccctcg
     2521 aacccggcca cgatccgctg gccggccatc tgatggttgg tcagcccggc ggaaaggttg
     2581 cgggtcaagg gcgggaaggg tgagctggcg ctcttcgact tcacaaaggg cccggccccg
     2641 gcggaattcg tcgtggtgcc cgaagaaggg cccgagtcac cgcccggcga ggaaagcagc
     2701 aggagagccg ccacaccggc cagcaaaagc gcgatgccga tcgccgcacg caggggtgat
     2761 tttctcaggg cggaagccat acatcgatga tacggatcgg ccccgaacgg cccgcctgcc
     2821 aaagcagaag agcgggtccg gatcaggcct gttcgatgac gtcgagggtc ttttcggtca
     2881 actcgaccgt cattcccccg gcttcaagca ggccggccag ggtt
//
LOCUS       DAOQMF010000161         2922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19412_length_2922_cov_2.936170, whole genome shotgun sequence.
ACCESSION   DAOQMF010000161 DAOQMF010000000
VERSION     DAOQMF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19412_length_2922_cov_2.936170"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLV77_03670"
     CDS             complement(<1..829)
                     /locus_tag="PLV77_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC14977.1"
                     /translation="MSATKRIVIAGVSTRWGSELARTLQRRPGVGEIIGIDSAPPTSD
                     LGRTEFVEADIRNPVISRILPSLEPDVVVHCGIVWYPEKGRPSRALHDINVIGTLLLL
                     AACEKTSGLQALIARGSAAIYGSAPGAPAFFTEDMARRLPLVTRFQRDIGELEGYFDN
                     FARRRPEITCCMLRFGVEVGPGLDTPFNRYLNLPVVPTRMGFDPRLQLLHVDDATGAL
                     AAATLNPVRGAVNVAPDGAISLSRLLRLCGKSAVGVPPVLVGVINRRIGNYLGSADLY
                     "
     gene            complement(881..1192)
                     /locus_tag="PLV77_03675"
     CDS             complement(881..1192)
                     /locus_tag="PLV77_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14978.1"
                     /translation="MAESGSEKSGGRGVPDGLKEAIEGAFAATGKTRAQAQDLTEKTR
                     NRAQGLVDEVTRRGSDARGTLDGLRLVSREEMRQLEERIDEISSRLAELERKSKAQPE
                     G"
     gene            1235..1936
                     /locus_tag="PLV77_03680"
     CDS             1235..1936
                     /locus_tag="PLV77_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14979.1"
                     /translation="MPVKMNPIEPAAGDAILVGDPRRAFALAQELTGQPKMSHQARGF
                     WGYKGLTDDGLRLTIQSTGVGGPSAVPVIGDLASQGVSRMIRLGTCLAIEPGLAAGTL
                     LLVSRAVVADGTSRVLGAGQDFVRPDQRLLGALGGLGREATISSHDLVRRYEPEGTGR
                     SDPGPALARDLQTAATLAMCRRLEIPAAALLIVAEDTAGNRLDEEAMWDLFRSAGKAV
                     LSRLARLEPNPQVEV"
     gene            complement(1938..2279)
                     /locus_tag="PLV77_03685"
     CDS             complement(1938..2279)
                     /locus_tag="PLV77_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14980.1"
                     /translation="MSAENDANEGIRDRVQSKGEKAAADLAQALIGSPAFGQAVSAAV
                     AARDRAAEAQRAAMGAMGVSSKDEEERLERRVRVLSDRLEETEDRLDAALDQIRELRA
                     ELKSSGSRKKT"
     gene            2419..>2922
                     /locus_tag="PLV77_03690"
     CDS             2419..>2922
                     /locus_tag="PLV77_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14981.1"
                     /translation="MTTRRDAPAATVAVLFFTLIFGSLTWFAASSSEAATTPVPCAPG
                     SASCFAAAEGKAAPAATDVPTSPATTPVQTPPDTGGGDQTGSDGGAVSTPGTGGVVPG
                     ETGGSGDGNGVTDPDGSTGEITPGGSEPDFVQQDPGLTGGAGPDSSVDTDPMTSDVLT
                     VPNFVIDQ"
BASE COUNT          469 a          998 c          979 g          476 t
ORIGIN      
        1 ggtagagatc ggcgctgccg aggtagttgc cgatccggcg gttgatcacg ccgaccaata
       61 ccggcggcac gccgaccgcg ctctttccgc aaagccgcag caggcggctg agcgaaatcg
      121 ccccgtcggg ggcgacgttg accgctcccc gcaccggatt cagggtcgcc gccgccagcg
      181 ccccggtcgc atcgtcgacg tgaagcagct gcagccgggg gtcgaaaccc atgcgcgtgg
      241 gcaccaccgg caggttcagg taacggttga agggggtgtc gaggccgggg ccaacctcga
      301 ccccgaagcg gagcatgcag caggtgatct cggggcgcct tctggcgaaa ttgtcgaaat
      361 agccctcgag ctcgccgatg tcgcgctgga agcgggtgac caggggcagg cggcgggcca
      421 tgtcctcggt gaagaaggcc ggggcgccgg gggcggaacc gtagatcgcg gctgaacccc
      481 gggcgatcag ggcctggaga ccggaggtct tttcgcaggc ggcgagcagc agcagggtgc
      541 cgatcacgtt gatgtcatgg agtgccctgg agggccggcc cttttccggg taccagacga
      601 tcccgcaatg aaccaccacg tccggttcca gcgagggaag gatgcgggag atgaccgggt
      661 tgcggatgtc cgcctcgacg aactcggtcc ggccgagatc ggaggtgggc ggggctgaat
      721 cgatgccgat gatctcgccg accccgggcc ggcgctgcag cgtccgcgcc agttccgacc
      781 cccagcgggt tgaaactccg gcgatgacta tccgttttgt cgcactcaat ttgaactccg
      841 gaccgggatc tggccaaaaa cggggccaaa tcgactaact ctaaccctca ggttgagcct
      901 ttgacttacg ctccaattcg gctagccgac tggaaatctc gtcgattctc tcctccagct
      961 ggcgcatctc ctcgcggctg accaggcgaa gcccgtcgag cgtcccccgg gcatcggatc
     1021 cgcgcctggt cacctcgtcg accagcccct gggcccggtt tcgcgtcttc tcggtgagat
     1081 cctgggcctg ggcccgggtc ttgccggtcg cggcgaacgc accctcgatc gcttctttga
     1141 gaccgtcggg aacgccgcgg ccgcctgact tctccgatcc gctttcagcc atgcggctag
     1201 tttacgacga aagaccgccc cgccggagaa tccgatgccc gtaaagatga acccgatcga
     1261 gcccgccgcc ggtgacgcga tcctggtcgg cgacccccgt cgcgccttcg ccctcgccca
     1321 ggagttgacc ggacagccga agatgagcca tcaggcccgc gggttctggg gctacaaagg
     1381 cctgaccgat gacggcctcc ggctgaccat ccagtcaacc ggcgtcggcg gcccgagcgc
     1441 cgttccggtg atcggcgacc tcgcttccca gggggtgagc cggatgatca ggctcggcac
     1501 ctgccttgcg atcgagcccg gactggccgc cggaacgctg ctcctggtga gtcgagccgt
     1561 ggttgccgac gggacttccc gggtgctcgg cgccggccag gacttcgtgc ggcccgatca
     1621 gcgtctgctc ggtgcgctcg gcggcctcgg gcgcgaagcg acgatctcga gccacgacct
     1681 ggtgcggcgt tacgaaccgg agggaaccgg ccggtccgat ccgggaccgg cgctcgccag
     1741 ggacctgcag accgccgcca cgcttgcgat gtgccgccgg ctggaaatcc cggccgcggc
     1801 gctgctgatc gtggcagagg acacggcggg gaaccggctc gacgaggaag cgatgtggga
     1861 tctgttccgg agcgccggca aggcggtgct cagccgattg gcaaggctag aacctaaccc
     1921 tcaagttgag gtttaggtca ggtctttttc cggcttcctg acgatttcag ctccgctcgc
     1981 agctcccgaa tctggtcgag tgccgcgtcg agccggtctt cggtctcttc gagacggtca
     2041 gaaaggaccc ggacccgccg ttcgagccgc tcttcctcgt ctttcgaaga gacgcccatg
     2101 gcacccatcg ccgcccgctg tgcttcggcg gcgcgatccc tcgcggccac cgcagcggag
     2161 accgcctgac cgaatgcggg acttccgatc agcgcctggg cgaggtcggc cgcggccttc
     2221 tcgcccttcg actggacccg gtccctgatt ccctcgttgg catcgttttc agctgacatg
     2281 acacgatctt aaagggcgct tcgcaggaag tgtcgaggta ccggcgaaag ccgttgcgat
     2341 acggttcctc tgccgcgtca cgggagggcg tcggccgtgt ctgttttagt tgggaagcca
     2401 aaggtggacc agggttcaat gactacacgc cgcgatgcgc cggccgccac agtagcggtc
     2461 ctctttttca cgctcatttt cggctccctg acttggtttg cggcgtcctc ttccgaagca
     2521 gccacgactc cggttccctg cgctccggga tcggccagct gcttcgcggc ggccgagggc
     2581 aaggcggcac cggccgccac cgacgttccg acctcccccg cgaccacacc ggtccagacc
     2641 ccgccggaca ccggcggcgg tgatcagacg ggcagcgacg gcggcgccgt gagcacaccc
     2701 ggcaccggcg gagtggtgcc cggcgagacc ggcggcagcg gcgacggcaa cggggtgacc
     2761 gaccccgacg gaagcaccgg cgagatcacc cccggcggca gcgaacccga cttcgtccag
     2821 caggaccccg gcctgaccgg cggcgccggg cccgacagca gcgttgacac cgacccgatg
     2881 acctcggatg tcctcacggt tcccaacttc gtgatcgacc ag
//
LOCUS       DAOQMF010000162         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19437_length_2920_cov_3.532286, whole genome shotgun sequence.
ACCESSION   DAOQMF010000162 DAOQMF010000000
VERSION     DAOQMF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19437_length_2920_cov_3.532286"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..465)
                     /locus_tag="PLV77_03695"
     CDS             complement(64..465)
                     /locus_tag="PLV77_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC14982.1"
                     /translation="MSDSATTVNNLGVAMFTVASQDDAIDFYTGKLGWELRGDQAFGE
                     DGSMRWVEVAPPGSGARLALNPPMRDEPGGSTIGVESVDVLGEHRHLREIGGIDVDEE
                     PMRTPGAPLMFGLRDPDGNNIYVVESPEVRD"
     gene            complement(599..670)
                     /locus_tag="PLV77_03700"
     tRNA            complement(599..670)
                     /locus_tag="PLV77_03700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(636..638),aa:Val,seq:cac)
     gene            759..>1116
                     /locus_tag="PLV77_03705"
     CDS             759..>1116
                     /locus_tag="PLV77_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14983.1"
                     /translation="MRGRLKELWGKPGFANVVALVALFIALGGVSYAAVQIPKNSVGT
                     KQLKKNAVNSAKVRNRSLLAADFKKGQLPRGATGAAGVTGSTGATGPAGTTGLTGAAG
                     PTGSAGEAGTTGATGTA"
     assembly_gap    1117..1139
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1140..1529
                     /locus_tag="PLV77_03710"
     CDS             <1140..1529
                     /locus_tag="PLV77_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC14984.1"
                     /translation="TAALGAVASLGSTTQLFSPLGLSTPVSSSSALQVATRAPGVNVK
                     LDNLSVLFDTPTGGDGSVFRTISIGTVDQAGTVTGILGCSVNGGGKICSNSNEVSVPS
                     GSLLVIRSAVSGTPPATMVHVGYTLGP"
     gene            1557..2423
                     /locus_tag="PLV77_03715"
     CDS             1557..2423
                     /locus_tag="PLV77_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNC14985.1"
                     /translation="MRSRDRRRSLAVRRTVALLVVLIALGIVAWAALNVSRLWKTDTH
                     GAELTETSIESKAVPGEHPVSVVVPDGADDGERGMLVFLHGRSGDDGSYTDDEEFFEA
                     LAGQGGDAPIVVFPDGGESSYWHDRDTGNWGSYVTDEVIPQMAGKFGADPDRVAIGGI
                     SMGGFGALDLAIQNPGMFCAVGGHSPALFLSGGESAPGAFDDAEDFDDNDVLATAAAD
                     PDAFSSQPLFLDAGKSDPFVPGVTELANSLEAAGVDATVKLNWPGGHDEDYWNAHWDE
                     YLAFYADALADC"
     gene            2456..2720
                     /gene="ffs"
                     /locus_tag="PLV77_03720"
     ncRNA           2456..2720
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLV77_03720"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            2756..>2920
                     /locus_tag="PLV77_03725"
     CDS             2756..>2920
                     /locus_tag="PLV77_03725"
                     /inference="COORDINATES: protein motif:HMM:NF022522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HNC14986.1"
                     /translation="MSERTWRVYLAGEIHSDWRDRVEAACEGLPVVFTAPVTDHPASD
                     AAGTSTLGDEE"
BASE COUNT          493 a          903 c          975 g          526 t
ORIGIN      
        1 atccctgaac tgaaagtacc gcccggacgg ttgcggcttt ctaccggact ttcgtccgga
       61 acctcagtcg cgaacctcgg gtgattcgac cacgtagatg ttgtttccgt cggggtcacg
      121 caggccgaac atcaacgggg cgccgggggt ccgcatcggt tcctcgtcca cgtcgattcc
      181 gccgatttcc ctcaggtggc gatgctcgcc gagcacgtca accgactcga ccccgatcgt
      241 gctgccgccg ggttcatccc gcatcggggg gttcagggcg agccgggctc cggagccggg
      301 tggtgcgacc tcgacccagc gcatgctgcc gtcctcgccg aaggcctggt cgcccctgag
      361 ttcccaaccg agcttcccgg tgtagaagtc aatcgcgtca tcctggctcg caacggtgaa
      421 catcgcgact ccgaggttgt tgactgtggt ggctgaatct gacatggatc ctccggtcgg
      481 tcgtggtctt atcgatccta tgcccggatc cgtaccggcg aatcaggcgc cccgctcggg
      541 gtcggggaaa tccgcggtcc cgttgcttcc aaacgacccg tcgggcttgc tgaccggatg
      601 ggcacggctg gtttcgaacc agcgacctct cgcgtgtgaa gcgagcgctc tcccgctgag
      661 ctacgcgccc gggaacgggc attgtagttg gacggcctga gagacgggta gcggctcaga
      721 ggtggaaata cccgggccag ccggataggt tcttcccaat gcgggggaga ctcaaggaac
      781 tctgggggaa gccgggcttc gcgaatgtcg tcgccctggt cgcgttgttc atcgccctgg
      841 gcggcgtttc gtacgcggcc gttcagatcc cgaagaacag cgtcgggacg aagcaactga
      901 agaagaacgc ggtcaactcg gccaaggtca ggaaccgttc cttgctcgcg gccgatttca
      961 agaagggaca gctgccaagg ggcgcgaccg gcgcggccgg ggtcactgga tcgactggtg
     1021 ccaccggtcc agctggtacg acagggttga ccggcgccgc cggtccgacc ggatcggcag
     1081 gcgaggcggg aaccaccggc gcgaccggaa cggcagnnnn nnnnnnnnnn nnnnnnnnna
     1141 ccgccgctct cggtgcagtc gcaagcctcg gatcgaccac ccagctcttc agcccgcttg
     1201 gattatcgac tccggtgagc tcatcgtcgg cgctccaggt ggcgacgcgt gctcctggcg
     1261 tcaatgtgaa actagacaac ctctcggtgc tgttcgacac tccaaccggc ggcgacggct
     1321 cagtcttccg cacgatttcc atcggcacgg tcgatcaggc gggaacggta acggggattc
     1381 tcggttgttc ggtcaacgga ggcgggaaaa tttgttccaa ctcgaacgag gtgagcgttc
     1441 cgtccggttc acttctcgtc atccgaagtg ccgtttcggg gactccgcca gcgacaatgg
     1501 tccacgtggg gtacaccctc ggcccttgaa tcggtcgtag ggttcggccc ttggccgtga
     1561 gaagcagaga cagaaggcga tcgctggccg tgcgccgaac ggtcgcactg cttgtcgtcc
     1621 tgatcgcctt gggcatcgtc gcgtgggcgg cgttgaacgt gagccgcttg tggaagaccg
     1681 acacccacgg ggccgagctg accgagacct cgatcgagtc gaaggcggta cctggcgaac
     1741 acccggtttc ggtggtcgtg ccggatggtg ccgatgacgg cgagcggggg atgctcgtgt
     1801 tcctccacgg tcgcagcggt gacgacggga gctacacgga tgacgaggag ttcttcgagg
     1861 cgctggcagg ccagggcggg gacgcaccga tcgtggtctt tcccgacgga ggcgagtcca
     1921 gctactggca tgaccgggac accggaaact ggggcagcta cgtcactgac gaggtgatcc
     1981 cgcagatggc agggaagttc ggggccgacc cggaccgggt tgcgatcggt ggaatctcga
     2041 tgggcggttt cggtgcgctc gacctggcga tccagaaccc ggggatgttc tgcgctgtcg
     2101 gcggtcactc gccggcccta tttctgagcg gcggggagtc ggctcccggc gccttcgatg
     2161 acgccgagga cttcgacgac aacgacgtgc tcgctaccgc cgcagccgac ccggatgctt
     2221 tctcgtcgca acccctcttc ctcgacgccg gcaagagcga ccccttcgta cccggcgtca
     2281 ccgaactggc gaactcgctc gaagcggccg gggtcgacgc caccgtgaag ctgaactggc
     2341 ccggcggcca cgatgaggat tactggaacg cccactggga cgagtacctc gctttctacg
     2401 ccgacgccct cgctgactgc tgaaccctgc gcggggcgct aggattccaa ccggaccgtg
     2461 ctaggcgggg agtcagcggt gccctgtcac ttgcaatccg ctttagcaag gccgaatgcc
     2521 gttctgaggg tctggccatc ggggtcagta caccgaggga ggtgttgacg gtcaggtccc
     2581 gcgcgacgca agcccgtgaa tcaggtcagg tccggaagga agcagcctta agcggaaact
     2641 acgggtgccg cgggtgagcc taacccgagc cgaactcgac ggacacgccc gatgacttcg
     2701 tttcgaaggc ggttgcacgg tccattttct ttcccttgac gggtaggttg ggcacatgtc
     2761 tgaacgaacg tggcgcgtct accttgccgg cgaaattcac tccgactggc gggatcgggt
     2821 cgaggccgct tgcgaggggc taccggtcgt tttcaccgcc ccggtgaccg atcaccctgc
     2881 ttcggatgcg gccggaacct cgaccctcgg agacgaggag
//
LOCUS       DAOQMF010000163         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19466_length_2918_cov_3.066364, whole genome shotgun sequence.
ACCESSION   DAOQMF010000163 DAOQMF010000000
VERSION     DAOQMF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19466_length_2918_cov_3.066364"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLV77_03730"
     CDS             <1..741
                     /locus_tag="PLV77_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNC14987.1"
                     /translation="GSQVVTAEDSVCRITASKGNLDPVAEGLISDVSVVSRLARKVIP
                     DSKVDWAAFEEDYDRIRDSISRVVPGFEDFNRKLEQEGTFILPHGPRDSRTFPTPSGK
                     ARFATAEVPVLEVPAGRLLLNTVRAHDQHNTAILGLNDRYRGIRKGRFVIFVSPEDLA
                     ELEIEDGQTVDVFSERTGEEDRVLRGYRAVAYPTKRGCAAMYFPEANELVHRSAVDRR
                     SNCPAYKDVIIRIEPGTNRVAGGRPVAN"
     gene            822..1217
                     /locus_tag="PLV77_03735"
     CDS             822..1217
                     /locus_tag="PLV77_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNC14988.1"
                     /translation="MSANEFKVAEAAALLGVSDDTIRRWAADGRLTLREGKGGVKVVD
                     GRELARLASESTLEAEGGATFSVRNRMHGIVTAVKTDAVMAQVDIQAGPFRLVSLISS
                     EAVEQLGIKVGKAVFATAKATNVGVEIAS"
     gene            1207..1989
                     /gene="modA"
                     /locus_tag="PLV77_03740"
     CDS             1207..1989
                     /gene="modA"
                     /locus_tag="PLV77_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC14989.1"
                     /translation="MRRKLAGRSLLAILATLAVAVALPACGSSDDSGSGDKEDLTVFA
                     ASSLTEAFTNLGKQYEAAHEGAVVKFNFGSSSDLAAQIDQGAPADVFASADEPNMQKV
                     VDSDMADGEPQDFASNVLEIAVPRDNPGKVTGLDDFAEKDLKLAVCDPEAPCGNAATE
                     VFGNAGVDASIDSYEPDVKSVLTKVELGDVDAGLVYDSDVLAAKDRIDSIAIPEQDQF
                     INVYPIVVVKGAGNAQGGQDFIDLVLGDDGQNELAKWGFRAP"
     gene            1968..2825
                     /locus_tag="PLV77_03745"
     CDS             1968..2825
                     /locus_tag="PLV77_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC14990.1"
                     /translation="MGIPGALRNVGGSSKDLAARQTRPPAAIALPAVFAVLFLILPLA
                     AVVIRLPWGSVGEILTRSDTVEALALSLGTASVATVLCVIFGVPLAWWLDWLGRSPRS
                     LVYRLTRTLVLVPLVLPPVVGGVALLMLLGRKGVIGGPINDLTGITLPFTTPAVVIAQ
                     AFVALPFLVLTVEGALRSIDRSLDEVALDLGATPTRSFLLVILPIVRPAVFSGAVLCF
                     ARALGEFGATITFAGNLPGVTRTMPIATYTTMQTDPDAALTLSLILLAVAVVILIAMR
                     DRWTRGAFG"
BASE COUNT          492 a          948 c          965 g          513 t
ORIGIN      
        1 ggcagccagg tcgttacggc tgaggattcg gtctgccgga tcaccgccag caagggcaac
       61 ctcgatccgg tcgccgaagg gctgatttcg gatgtgtcgg tggtttcccg gctggcccgc
      121 aaggtgatcc ccgattcgaa ggtcgactgg gccgcctttg aagaggacta tgaccggatc
      181 cgcgactcga tcagccgggt ggttccgggg ttcgaggatt tcaaccggaa gcttgaacag
      241 gaagggacct tcatcctgcc ccatggccct cgtgacagcc ggaccttccc gaccccctcc
      301 gggaaggcgc gcttcgcgac cgctgaagtg ccggtactgg aggtgcccgc cggccgcctg
      361 ctgctgaaca cggtccgggc ccatgaccag cacaacaccg cgatcctcgg tctcaacgac
      421 cgttaccgcg gaatccgcaa ggggcgcttc gtgatcttcg tcagtccgga ggacctggcc
      481 gagctggaga tcgaggacgg ccagacggtc gacgtcttca gcgaacggac cggggaggag
      541 gatcgggtcc tgcgcggcta ccgggcggtc gcctacccga cgaagcgggg ttgcgcggcg
      601 atgtactttc ccgaagccaa cgaactggtc caccgctccg cggtcgaccg gaggagcaac
      661 tgcccggctt acaaggacgt gatcatccgg atcgaacctg gcacgaaccg ggttgccggt
      721 ggtcgcccgg tcgcaaacta ggaacaaaac ctcactacaa gccgcgaata cggcttatat
      781 ttgggtattt ggccgcaatc aggtgttgat agtgtggccg catgtcagcc aacgaattca
      841 aggtcgccga ggcggctgcc ctgctcggag taagcgatga cacgatccgc cgctgggccg
      901 cagacggccg gttgaccctc cgggagggga agggcggggt caaggtcgtc gatggccgcg
      961 aacttgcccg gctggcctcg gaatcaacgc tggaggccga aggcggtgcg acgttttcgg
     1021 ttcgcaaccg catgcacggg atcgtgaccg cggtcaagac cgacgcagta atggctcagg
     1081 tcgacattca ggccggtccc ttccggctcg tttcgctgat cagcagcgaa gcagtcgagc
     1141 agctcggcat caaggtcggc aaggccgtgt tcgcaactgc aaaagcaacc aacgtgggag
     1201 tggaaattgc gtcgtaagct ggccggcaga agccttctgg cgatcctcgc gacgctcgcc
     1261 gttgcggtag cgcttccggc ctgcggcagt tcggatgatt cgggttccgg tgacaaggag
     1321 gatctgaccg tcttcgcggc atcctcgctg accgaagcct tcaccaacct cggcaagcag
     1381 tacgaagccg cgcatgaggg cgcggtggtc aagttcaact tcgggtcgag ctcggacctc
     1441 gccgcccaga tcgaccaggg cgcgccggcc gacgtcttcg cctcggccga cgagccgaac
     1501 atgcagaagg tcgtggattc ggacatggcc gacggcgagc cgcaggactt tgccagcaac
     1561 gtcctcgaga tcgcggtccc cagggacaat cccggcaagg tgaccggtct cgatgacttc
     1621 gccgaaaagg acctgaaact ggcggtctgc gaccccgaag ctccctgcgg aaacgccgcc
     1681 acggaggtct tcgggaacgc cggagtcgac gcttcgattg acagttacga gcccgacgtg
     1741 aaatcggtgc tgaccaaggt agaactcggt gatgtcgatg ccgggctcgt ctatgactcg
     1801 gatgtcctcg cggccaaaga caggatcgac tcgatcgcga ttcccgagca ggaccagttc
     1861 ataaacgtct acccgatcgt cgtggtcaag ggtgccggca acgcccaggg cggccaggac
     1921 ttcatcgacc tcgtgctcgg cgacgacggt cagaacgaac tggcgaagtg gggattccgg
     1981 gcgccctgag aaacgtcggc gggtcctcga aggacctggc ggcccgccag accaggccgc
     2041 cggccgcgat cgcgctgccg gcggttttcg cggttctctt tttgatcctg cccctggcgg
     2101 cggtggtcat caggttgccc tggggttcgg tcggggagat cctcacccga agcgacaccg
     2161 tcgaggcgct ggccctttcg ctcggcacgg cgtcggtcgc gaccgttctc tgcgtgatct
     2221 tcggagtacc cctggcctgg tggctcgact ggctcggccg ctcaccccgc agcctggttt
     2281 atcgccttac caggactctc gtcctggtcc cgctggtgct gcccccggtg gtcggcgggg
     2341 tggccttgct gatgctgctc gggcgaaagg gggtgatcgg cggcccgatc aacgacctga
     2401 ccggcatcac cctgcccttc acgactcccg cggtcgtgat cgcccaggca ttcgtggccc
     2461 tgcccttcct ggtgctcacg gtggaagggg cgctgcggtc gatcgatcgc agcctcgatg
     2521 aagtggcgct ggacctcggg gcgacaccga cccggtcctt cttgctggtc atcctgccga
     2581 tcgttcgacc ggccgtcttt tccggggccg tgctctgctt cgccagggcc ctcggcgagt
     2641 tcggcgcgac gatcaccttc gccggaaatc tccccggtgt cacccggacc atgccgatcg
     2701 cgacctacac caccatgcag accgaccccg acgcggccct caccctttcg ctgattctgc
     2761 tcgccgtcgc ggtggtgatc ctcatcgcga tgcgcgaccg ctggaccaga ggcgccttcg
     2821 gctgatcggc cggtccgccg cgggttgaag ccgcctggtc ggggctagcc gaccgagagg
     2881 gcggccgcgc cagccagcac cacggcgatg ccggcgat
//
LOCUS       DAOQMF010000164         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19473_length_2917_cov_4.809923, whole genome shotgun sequence.
ACCESSION   DAOQMF010000164 DAOQMF010000000
VERSION     DAOQMF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19473_length_2917_cov_4.809923"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1608)
                     /locus_tag="PLV77_03750"
     CDS             complement(97..1608)
                     /locus_tag="PLV77_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC14991.1"
                     /translation="MEPTAATESIAVMNPATGEQVGSVPVDSAGDVAAAVARIRGNQP
                     AWEALGFKGRRRWLNELRDWLLDNSDRVADTLKSETGKVRAEATIETPYLADQINFYG
                     SKAEKFLGEQKVRPNTMLAATRKLRLQYRPYQVVGLISPWNFPLVLSLGDAIPALMAG
                     AAVVMKPSEITPLGLNEIVDAWRNEIGAPDVLHVVNGGAETATALVDHSDFVGFTGSD
                     VTGKKVMARAAETLTPVSLELGGKDPMVVLEGGNMDRAVNGATWGSMLNSGQMCISVE
                     RIYVEDGAYDEFVEKLTDSVGELKQGIDGETAACDVGAMTMLSQLEKVEKQVDDAVEA
                     GARVLTGGKRGEGPGQWFEPTVLVDCTPDMSVMEDETFGPVVTVTRVSDEEEAIRLAN
                     DSRYGLCASVFGPRGRAEKVARRIEAGAVNVNDIVFNMLAPGLPMGGWKESGIGYRNG
                     EYGIRKYCRSEAIVSARTLLRSEPLWYPYTPERHALVSKVTKFIGGRGLRRFR"
     gene            complement(1732..>2917)
                     /locus_tag="PLV77_03755"
     CDS             complement(1732..>2917)
                     /locus_tag="PLV77_03755"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC14992.1"
                     /translation="LFAALAGFVLFLTAGDSAGAAPKLGYTRIATAREPTALVSPPGY
                     PRMLFVTERAGFVRVIANGRKLKQPLLDLTSKVGSSNIERGLIGLAFAPDFRTSGRFY
                     VDYTAKNGNSIVAGYRTVPGQPTRLARGGGRLVIQIPRVNNKGNHNGGHLAFRGNLLY
                     IAVGDGFDPGDAPDHARNLNSLRGKILRIDPRPDQGTGKPYRIPAGNPFVGLPGRDEI
                     FAYGLRNPFTFSFFNSGGEAMMSIADVGQLRYEELNVLPFAEARGANFGWKGFEGFEP
                     YNCGESLCPNGADPVVATGLKWPQLVYSHDEGCAIVGGLKISDPKLTAVRGRIIYGDF
                     CSGRIRTALPHSPTIVDDRPIGFSLPARHGVTLLNGFGVDGLKRLYAFSHLGGIYRLI
                     QR"
BASE COUNT          460 a          935 c         1007 g          515 t
ORIGIN      
        1 tgagtgcgct atgcggcaat aagtcgtatc tcgatatccc cggaaccggg tcggcgtagc
       61 cgcgggggcg gcgttaccag cggggtttcg gagggactat ctgaagcggc gaaggccgcg
      121 gccgccgatg aacttggtga ctttgctgac cagggcgtgg cgctccgggg tgtacgggta
      181 ccagagcggt tccgagcgga gcagggtgcg ggcggaaacg atcgcctcgg aacggcagta
      241 cttgcggatc ccgtactcgc cgttgcggta gccgatcccc gactccttcc agccgcccat
      301 cggcaggccc ggcgccagca tgttgaagac gatgtcgttg acgttgaccg cgccggcttc
      361 gatccgccgg gccaccttct cggcccggcc gcgggggccg aagaccgagg cgcagagtcc
      421 gtagcggctg tcgttggcga ggcgaatcgc ctcttcctcg tcgctcaccc gggtcacggt
      481 gacgacggga ccgaaagtct cgtcctccat cacgctcata tcgggggtgc agtcgaccag
      541 cacggtcggc tcgaaccact gacccggacc ttcgccgcgc ttgccgccgg tcagtacccg
      601 ggctccggcc tcgaccgcgt cgtcaacctg cttctcgacc ttctcaagct gggacaacat
      661 ggtcatcgcg ccgacgtcgc aggcggcggt ctcgccgtcg atcccctgct tcagctcgcc
      721 gacgctgtcg gtcagcttct cgacgaactc gtcgtaagcg ccgtcctcga cgtagatccg
      781 ctcgaccgag atgcacatct ggcccgagtt gagcatgctg ccccaggtcg caccgttgac
      841 ggcgcggtcc atgttgccgc cttcgagaac aaccatcggg tccttgccgc cgagttcgag
      901 gctgaccgga gtcagggtct cggccgcgcg ggccatcacc ttcttgccgg tcacgtcgga
      961 gccggtgaag ccgacaaagt cggagtggtc gaccagggcg gtcgcggttt cggcgccacc
     1021 attgacgacg tgaagcacgt cgggggcgcc gatctcgttg cgccaggcgt cgacgatctc
     1081 gttcaggccg agcggagtga tctcggaagg cttcatgacc acggcagcgc cggccatcag
     1141 cgccgggatc gcgtccccga gcgagagcac gagcgggaag ttccaggggc tgatcagacc
     1201 gaccacctgg taagggcggt actggagcct gagcttgcgg gtcgcggcca gcatcgtgtt
     1261 cggcctgacc ttctgctcgc cgaggaactt ctcggccttg gacccgtaga agttgatctg
     1321 gtcggccagg tagggggtct cgatcgtcgc ttcggcccgc acctttccgg tttcggactt
     1381 gagcgtgtcg gcgacccggt cggaattgtc gagcagccag tcgcgaagtt cgttcagcca
     1441 gcggcggcgg cccttgaagc caagcgcctc ccaggcaggc tggttgccgc ggatgcgggc
     1501 gaccgccgcg gcgacgtcgc cggccgagtc gaccgggacc gacccgacct gctcgcccgt
     1561 ggcggggttc atcactgcga ttgactcggt tgctgccgtc ggttccacct gctgctcccg
     1621 tctctcgact ctcttcgcct ccgggccggt ttgcccttgg ggactgtcgt tacaggttag
     1681 cgggggccgg aaggaattca ccgaatcgat ccgctttgcc ccggggcggg gtcagcgctg
     1741 gatcagccgg taaatgccgc cgaggtgact gaaggcgtaa aggcgcttga ggccgtcgac
     1801 gccgaagccg ttcagcagcg tgactccatg cctggccggc agcgaaaagc cgatcggcct
     1861 gtcatcgacg atggtcgggc tgtgtggcag ggccgttcgg atccggccgc tgcagaagtc
     1921 gccgtagatg atccttcccc tgaccgccgt cagtttcggg tcgctgatct tcaggccgcc
     1981 gacgatcgca cagccctcgt cgtgggagta gacgagctga ggccatttca ggccggtggc
     2041 aaccaccggg tcggcgccgt tcgggcaaag tgactcgccg cagttgtagg gctcgaagcc
     2101 ctcgaaaccc ttccagccga aattggcccc ccgcgcttcg gcgaagggga ggacgttcag
     2161 ctcctcgtag cgaagctggc cgacgtcggc gatcgacatc atcgcctcgc cgccggagtt
     2221 gaagaagctg aaggtgaagg ggttgcgcag cccgtaggcg aagatctcgt cgcgtccggg
     2281 aaggccgacg aagggattgc cggccgggat ccggtacggc tttccggtcc cctgatccgg
     2341 ccgggggtcg atccgcagga tcttgccgcg caggctgttc aggttccggg cgtgatccgg
     2401 ggcgtcacct gggtcgaagc cgtcaccgac cgcgatgtag agaagattgc cgcgaaaggc
     2461 gaggtggcct ccgttgtggt tgcccttgtt gttgacccgc gggatctgga tcacaagccg
     2521 gccgccgccc cgcgcaagcc gggtcggctg gccgggaacg gtccggtacc cggccacgat
     2581 cgagttcccg ttcttcgccg tgtaatcgac gtagaagcgg ccgctggtcc gaaaatccgg
     2641 ggcgaaagcg agtccgatca gacccctttc gatgttgctc gaaccgacct tcgaagtgag
     2701 gtcgagcagg ggctgcttca gcttcctgcc gttggcgatt acgcgaacga aaccggcccg
     2761 ctcggtgacg aagagcatcc gcgggtagcc gggcggggaa acgagggcgg tcggttccct
     2821 ggcggtcgcg atcctggtgt aaccgagttt cggtgcggcg ccggccgaat cgcccgcggt
     2881 caggaagaga acgaaaccgg ccagggccgc gaacagg
//
LOCUS       DAOQMF010000165         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19485_length_2916_cov_5.093324, whole genome shotgun sequence.
ACCESSION   DAOQMF010000165 DAOQMF010000000
VERSION     DAOQMF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19485_length_2916_cov_5.093324"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLV77_03760"
     CDS             complement(<1..408)
                     /locus_tag="PLV77_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HNC14993.1"
                     /translation="MTITESSAQTTIPHDYSKRAMVFGGRSSQDLAAKIAGKLSLAVG
                     DVTLKTFSDGEIYGRYEESIRGADVFLVQSTCANVETDMTPNDALMELMIMADAAQGA
                     SAHRIIAVMPWFGYARQDKKSAPREPITSRVVAR"
     gene            complement(405..1808)
                     /gene="glmU"
                     /locus_tag="PLV77_03765"
     CDS             complement(405..1808)
                     /gene="glmU"
                     /locus_tag="PLV77_03765"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNC14994.1"
                     /translation="MTGAPVTALIMAAGQGTRMRAALPKVLHPVCGRPMIAWPVLAAR
                     EAGADRVAVIVSPERDLSRALPDGVETVEQVESNGTGGAVRSALEIVRESPRVLVLSG
                     DVPLISAEVITGLLASQDESGASATVMTAILDDPGSYGRIVRGGDGTIRKIVEAKAEG
                     DATREELAIREINSGTYVFDGESLASALGELSSDNAQGEYYLTDVIAVMRSRGKLVTA
                     HVSKDPAINLGVNNRADLAVVEAEARQRILRRHMLDGVTIVDPATTWIDAGVTIGQDS
                     RIEPGTSLRGATEIAEEAVIGPHSTIIDSGIGKGCQVIQSYLNLCELAEGCSVGPFAY
                     LRPGAKLEDGAKAGTFVEIKNSTIGKNSKVPHLSYIGDTEIGENSNLGAGTITANYDG
                     AEKHRTKIGSDVKIGVDTMLVAPVEVGDGAYTGAGAVIRDNIPPGALSVSENDQRNVD
                     DYAERLADRKAEGESSQ"
     gene            complement(2015..2572)
                     /locus_tag="PLV77_03770"
     CDS             complement(2015..2572)
                     /locus_tag="PLV77_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNC14995.1"
                     /translation="MEYNATPEKEVKRASGIFTGAGILMILVGCFAIVAPHIVGQAFA
                     LLVGISFVIASVFIFVWAFSAEGAGTVIARIAWALVALIVGLIIVTHTDLSAKAFTVI
                     LIAYFLAAGVVRLGAAFAQRGVPGAGWLGLNGALSLLIGLIFVASWPNSAEWAIGLLI
                     GIDLIFSGWTLILLSQEAKSQALGS"
     gene            2617..2688
                     /locus_tag="PLV77_03775"
     tRNA            2617..2688
                     /locus_tag="PLV77_03775"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2650..2652,aa:Gln,seq:ttg)
BASE COUNT          514 a          925 c          949 g          528 t
ORIGIN      
        1 gcgggcaacc acgcgggaag tgatcggctc gcggggggcc gacttcttgt cctggcgggc
       61 gtacccgaac cagggcatga cggcgatgat ccggtgggcg gaggcgccct gggcggcgtc
      121 ggccatgatc atcagttcca tcagggcgtc gttcggcgtc atgtccgtct cgacgttggc
      181 gcaggtcgac tggacaagga agacgtcagc gccgcgaatc gattcctcgt agcggccgta
      241 gatctcgccg tcggaaaagg tcttcagggt gacgtcaccg accgcaagcg aaagcttgcc
      301 ggcgatcttc gcggccaggt cctgggagct ccggccacca aacaccatcg cccgcttcga
      361 atagtcgtgc gggatggtcg tctgggctga actctcggta atggtcactg actgctttcc
      421 ccttctgcct tgcggtcggc gagccgctcg gcgtaatcat caacgtttct ctgatcgttc
      481 tcgctcaccg agagggcccc gggaggaatg ttgtcgcgga tcaccgcgcc ggcgccagtg
      541 tacgcgccat cgccgacttc caccggcgcc accagcatcg tgtcgacgcc gatcttcacg
      601 tcggagccga tcttcgtgcg gtgcttctcg gcgccgtcgt agttggcggt gatcgtgccg
      661 gccccgagat tcgagttttc accgatctcg gtgtcaccga tgtaggacag atgcgggacc
      721 ttcgagttct tgccgatcgt cgagttcttg atctcgacga aggtgcctgc cttggctccg
      781 tcctcgagct tcgccccggg gcgcaggtag gcgaagggac cgaccgagca accctccgcc
      841 agttcgcaga ggttcagata ggactgaatc acctggcaac ccttgccgat ccccgaatcg
      901 atgatcgtcg aatgcgggcc gatcactgcc tcttcggcga tctcggtcgc gccgcgcagg
      961 gaggtgccag gctcgatccg ggagtcctgc ccgatcgtca ccccggcatc gatccaggtc
     1021 gtggcggggt cgacgatcgt tacgccgtca agcatgtgcc ggcggaggat ccgctgacgg
     1081 gcctcggcct cgaccacggc caggtcggcc cggttgttga ccccgaggtt gatcgccgga
     1141 tccttcgaga catgggccgt gaccagtttc ccgcgggagc gcatcaccgc gatcacgtcg
     1201 gtcaggtagt actcgccctg ggcgttgtcg ctggagagct caccgagggc cgaagcaagc
     1261 gattcgccgt cgaagacata ggtcccggag ttgatctcgc ggatcgccag ttcctcgcgg
     1321 gtagcgtccc cctcggcctt cgcttcgacg atcttgcgga tcgtgccgtc gccaccgcgg
     1381 acgatccggc cgtaggagcc ggggtcgtcg aggatcgcgg tcatcaccgt ggcggaggct
     1441 ccgctttcat cctgggaggc gagcagcccg gtaatcacct cggccgaaat caggggtaca
     1501 tcgccggaga gcaccagcac ccgcggggac tcgcgaacga tctcgagggc ggacctgacc
     1561 gcgccgccgg ttccgttcga ttcgacctgt tcgaccgttt caaccccgtc cggcagggcc
     1621 cgggaaagat cccgctcggg ggaaacgatc accgccaccc ggtcggcccc ggcttcgcgc
     1681 gcggcgagca ccggccaggc gatcatcggc cggccacaga cgggatgaag caccttgggc
     1741 agggcagccc gcatgcgggt gccctgaccg gccgccatga tcagcgctgt tacgggggcc
     1801 ccggtcacga gggacattct aagtcggcga gcttcggcat gccgacttga gtcggcatgc
     1861 ctcgccccgc ctccaagtta aacccgccgg cccatcaaac gaattgatgg ttgggccggc
     1921 tcagctagag gttcttccgg cccgattatc tgggggtgat ctgggttgaa gttattcgtt
     1981 ccatgtgcga ataacttcaa tgcagatggg agcgttagct gccgagggcc tgggacttgg
     2041 cttcctggga gaggaggatc agggtccagc cggagaagat caggtcgatg ccgatcagga
     2101 ggccgatcgc ccactcggcc gaattcggcc aggaggcgac gaagatcagg ccgatcagga
     2161 gcgaaagtgc tccgttcaag ccaagccagc ccgccccggg cacgccccgc tgggcgaaag
     2221 cggctccgag ccggacgacg ccggccgcga ggaagtaggc gatcaggatc acggtgaagg
     2281 cctttgcgct caggtccgta tgggtgacga ttatcaaccc gactatcaat gcgaccaggg
     2341 cccaggcgat cctcgcgatc acggttccgg ctccctcggc cgagaaagcc cagacgaaga
     2401 tgaacaccga cgcaatgacg aaagagatcc cgaccagcag cgcaaatgcc tgaccgacga
     2461 tgtggggcgc gacgatcgcg aaacaaccga ccaggatcat caggatcccg gccccggtga
     2521 aaatcccgga tgcgcgtttg acttccttct ccggtgttgc gttgtattcc atggccaaag
     2581 cctatcctcg ccagcggagg tagagtccgg cgcgattccg ggatggtgta atcggtagca
     2641 caccggtttt tggtaccggc agtggaggtt caagtcctcc tcccggagtt tggagtacct
     2701 ctatgtttgg gaatcgcggg acgcggttat gggtgcaggg tcagtctcgt tttcaggcaa
     2761 tagggacatc acccggagga atcgatgcga aagctcgcag tcgcagttgc ggtagccgtc
     2821 accaccttct tcgcccaggg cattcaggcc caggccgcgg tcaccgacgc cttcgacggc
     2881 gcgatcacct gcaagaccca gccggcgatc ccctct
//
LOCUS       DAOQMF010000166         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19507_length_2914_cov_3.916055, whole genome shotgun sequence.
ACCESSION   DAOQMF010000166 DAOQMF010000000
VERSION     DAOQMF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19507_length_2914_cov_3.916055"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..2016)
                     /locus_tag="PLV77_03780"
     CDS             complement(268..2016)
                     /locus_tag="PLV77_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNC14996.1"
                     /translation="MDARDGMDLKYSVLLPPGDGPFPAIMNYSGYDPGSIGGTAYQAG
                     NTTMDPELDAKLLKAGYAVVGVNMRGTGCSDGNFSLFANEWGTDGYDAVEWTADQPWS
                     NGKVGMANWSYAGLSQILTAVNNPPHLKAIAPGMAVTDQWRDVASPGGVTNWLFPYGW
                     GLFIQQRWNAAKTSAEVEGDTKCIDNIAAHNAGFNAVSPYREGVERPHVDNKPGRTNH
                     VWRQVHKIKAAVISMVAWQDEATGPRAGYYQNRLDPKRSYLVGTNGPHDIYMADRWQA
                     MLIPFFDRYVKGKKNGFEKKPHVRLWFDTKTPDGSSTKSGNLRKSTPGTVLTQKSLPI
                     KVKPLRLSLRSSGSLTTNKPRKPEPASSYDFPTVSPNVNPDYAEPGDTDWQEDPADPN
                     GTVAFTTAPLQRTLTFQGPASVDLWVSATTPDADIQATITEVRPDGKEVYVNRGWLRL
                     SERALDKKLSTPTLPFLKRTKGAVQMLKDSKPVYGRVEIMQFAHIFRKGTSIRVLLDT
                     PSTTGGYGFEGPRDDTKIKVFHGPNRNSKLVLGLMRVGGIKTPKPACDTVLRQPCREN
                     TFSIPTGKGPDGSTAR"
     gene            complement(2404..>2914)
                     /locus_tag="PLV77_03785"
     CDS             complement(2404..>2914)
                     /locus_tag="PLV77_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNC14997.1"
                     /translation="WAYAGLPPTPPRSYHSILELGMLGTGIPSAIGSKIGCPDRDVVC
                     VTGDGAAGFNIMEMQSAARDSIPVTVIVFAEGSWTMEEPNELALFNRTFGTEQGETRW
                     DLVAEGLGCHGEFVDQLGDLGEALVRCRDSGRPSVVCLKTNRDANRVIPPAMAARWME
                     VQAGPIPGV"
BASE COUNT          513 a          884 c          904 g          613 t
ORIGIN      
        1 catttgaccg atcctctcaa agcaattggc ctccaacaaa cccggggcgg ttcaattcgc
       61 gacgggacgt acttggtgac aagcgaagtt gagccaccga gttttttcgc gacagctcga
      121 acagtaactc cacgggttcc ctcgtcacga acgagttgaa gggtcgcttc tgcaatcgcc
      181 gacaggcgct cttgaacgtt gatttcaggt ggcactaacg gcagcctacg cggtcgtttc
      241 tccgcgtagg ctgccgacag gccgctctta cctggcagta gaaccgtcag gccccttgcc
      301 cgtgggaatc gagaatgtgt tctcacggca aggctgccgc agcacggtgt cacaggcagg
      361 cttcggtgtc ttgatgccgc cgaccctcat cagtccaagc acgagcttgg agttccggtt
      421 cggtccgtgg aacaccttga tcttggtgtc gtcccgcggg ccttcgaatc cgtatccacc
      481 ggtggtgctc ggcgtgtcaa ggagaaccct gatgctcgtg cccttacgga agatgtgggc
      541 gaactgcatg atctccactc gtccgtagac gggcttagag tccttcagca tctgaaccgc
      601 gcccttggtc ctcttcagga acggaagggt gggggtcgag agctttttgt cgagtgccct
      661 ttcggagagg cgcagccaac cgcggttgac gtagacctcc ttgccgtcag gccggacctc
      721 ggtgatcgtg gcctgaatgt cggcgtccgg agtcgttgcc gacacccaga ggtcaacgct
      781 ggccgggccc tggaacgtca gagtccgctg gagcggagcg gtggtgaaag cgaccgttcc
      841 gttcgggtca gcaggatcct cttgccaatc cgtgtcgccg ggttcggcgt agtcaggatt
      901 gacgttcggg ctcaccgtcg ggaagtcata cgacgaggcc ggttccggct tcctcggctt
      961 gttcgtcgtc aggcttccac ttgatcgaag tgacagccta agcggcttga ccttgatcgg
     1021 caggctcttc tgggtcagga cagttccggg ggtcgacttt cggaggttgc cactcttggt
     1081 agaagatcca tccggggtct tcgtgtcaaa ccacaggcgg acgtgcggct tcttctcgaa
     1141 gccgttcttc ttgcccttta cgtagcgatc gaagaacgga atcagcatcg cctgccagcg
     1201 gtctgccatg tagatgtcgt gcgggccgtt ggtgccgacg aggtaggacc tcttcggatc
     1261 gaggcggttc tggtagtagc ccgctcgagg tccggtcgct tcgtcctgcc aggcaaccat
     1321 cgagatgacc gcggccttga tcttgtggac ctgacgccag acgtgatttg tccgaccggg
     1381 tttgttgtcg acatgtggac gctcgacacc ctctcgatag gggctgacgg cgttgaaacc
     1441 ggcattgtgg gcggcgatgt tgtcaatgca cttggtgtcg ccttcaactt ctgcgctggt
     1501 cttggcggcg ttccaccttt gctggatgaa gagaccccag ccgtagggga atagccagtt
     1561 ggtcacaccg cccgggctgg cgacgtcgcg ccactgatcg gtcacggcca tgccgggagc
     1621 gatcgccttc aggtgcggag gattgttcac cgcagtcaag atctgactta gccccgcata
     1681 cgaccaattc gccattccga ctttgccgtt cgaccacggc tgatcggccg tccactcgac
     1741 ggcgtcgtag ccatccgtgc cccattcgtt cgcgaacaag ctgaagttgc cgtccgagca
     1801 tcctgttccg cgcatattca cgccgaccac cgcgtaccca gctttgagca gcttcgcgtc
     1861 aagttcaggg tccatggttg tattgccagc ttgataggcg gtgccaccga tgcttccggg
     1921 gtcatagccc gagtaattca tgatcgccgg gaacggtccg tcgccgggtg gaagaaggac
     1981 gctgtatttc aggtccatac cgtctcgggc atccatgtat ccggtcaagc gctccgccca
     2041 gaccccattg acgggcaggt aattaccggg cttgggaagg tcggcccgtt gactgtcagg
     2101 caacgggttt gccgcttggg ccagcgagct gaataccagc agtccgacga ctgccaaagc
     2161 aattgcgact gaccaagccg aagtccgctt caggaccagg agtccagctt cagtttgcac
     2221 cacggtaacg cactcctcct cctagacaac aatgttgtct aaccatacac aacgctgttg
     2281 cctgaaaggt agtttgatcc tgtacctggt cttggccgtc cacttgaact gaggtattcc
     2341 gcccgcagca ggctggtgcg acgcccagta tccggagggc gcgcttcggg atatgggccg
     2401 ggttcagacc cccgggatcg ggcccgcctg gacctccatc cagcgggcgg ccatcgccgg
     2461 ggggatgacc cggttcgcat cccggttcgt cttcaggcag acaaccgaag gccggccgga
     2521 atcacggcac cggaccaggg cctcgcccag gtctccgagt tgatccacga attcgccgtg
     2581 gcatccgagc ccctcggcga ccagatccca tcgggtctcg ccctgctcgg taccgaaggt
     2641 ccggttgaag agggcgagtt cgtttggttc ctccatggtc caggagccct cggcgaagac
     2701 gatcacggtg accgggatcg agtcgcgcgc ggctgactgc atttccatga tgttgaaccc
     2761 ggcggcgccg tccccggtca cgcagacgac atcccggtcg ggacagccga tcttcgatcc
     2821 gatcgcggaa ggaatgccgg ttccgagcat gcccagctcg aggatgctgt ggtacgaacg
     2881 gggcggggtc ggcggcaggc cggcgtaggc ccag
//
LOCUS       DAOQMF010000167         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19513_length_2914_cov_2.896817, whole genome shotgun sequence.
ACCESSION   DAOQMF010000167 DAOQMF010000000
VERSION     DAOQMF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19513_length_2914_cov_2.896817"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1711)
                     /gene="purH"
                     /locus_tag="PLV77_03790"
     CDS             complement(125..1711)
                     /gene="purH"
                     /locus_tag="PLV77_03790"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNC14998.1"
                     /translation="MDPTNGPVKVRRALISVSDKTGLADFAKGLADLGVEILSTGGTA
                     RALRDAGLTVIDVSEFTGQREIMDGRVKTLHPKIYAGLLARRSDPAHMEVLTEEEIPP
                     IDLVCVNLYPFEQTIAREDVGPQEAIENIDIGGPTMIRAAAKNHEDVVVLVKPECYDA
                     VIEEMNQTEGEVSASTRHWLANEAFAQTARYDAAISAWFSESYEAFPEHRVLAFEKQL
                     DLSYGENPHQRAALYSQVGLDSNVLTGMSKHHGKALSFNNVLDLDACRKLLADLEGPA
                     CVIVKHNNPCGVALGDSGLDAYERARDCDPVSAFGGVIAINQVIDVPLAEALHKNFVE
                     VLVAPGYEPEAMEILQQKENIRIFTDEAPPFPDSREKDIKRVRGGILIQDRDGEVEQR
                     EMMEVVTKAQPSEQAWIDMLFAWTVCRHVKSNAIVFAKDGVTLGIGAGQMSRVDSSRI
                     AIEKCRDAHGDEADDLLAGCAMASDAFFPFADGPMVGIEAGATSVIQPGGSKRDGEVI
                     EACDETETAMVFTRHRHFRH"
     gene            complement(1765..2403)
                     /gene="purN"
                     /locus_tag="PLV77_03795"
     CDS             complement(1765..2403)
                     /gene="purN"
                     /locus_tag="PLV77_03795"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNC14999.1"
                     /translation="MTPSSPFRVVVLASGGGTNLQAVLDQLHGRGEVEVVGVGSDKPA
                     AYALERATAAKVPTAVFPRDEYEDREARDAAMADWIESTGAELVALAGYMQLVSASFV
                     ERFRGQIVNVHPALLPSFPGLDAIGQALEQGVKVTGVTVHFVDEGTDTGPVIIQRAVE
                     VPANWDREELEVAIHAIEHEIYPEAIGMIARGEVKIADDEPRKVIVTQTRGK"
     gene            2516..>2914
                     /locus_tag="PLV77_03800"
     CDS             2516..>2914
                     /locus_tag="PLV77_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15000.1"
                     /translation="MILSLLLVAAVCPSPAEAIQYFGKKPGKWEAEIEIRNGFIRSMH
                     LKLPSNCRDQERPPGLDLEINRDSKLRLRRYGGFYRESVTRWSKSVLKGRVIGNRVQG
                     SLWFADLGDYVQCWTGTGEKPSWVRFAARAV"
BASE COUNT          472 a          953 c          963 g          526 t
ORIGIN      
        1 aacctcgcgg tagacggccc aaccgttccc ggatgtagtg ggccggcggg ccagcccgat
       61 gggtcggtcg aagcagaagg tgtcaaagct ataggagcgc gggcaaagcc ccgcgcgact
      121 gcagttaatg ccggaagtgg cggtgccgcg tgaagaccat cgcggtctcc gtttcgtcgc
      181 aggcctcgat cacttcgccg tcgcgctttg agccgccggg ctgaatgacc gaggtcgccc
      241 cggcttcgat tccgaccatc gggccgtcgg cgaaggggaa gaaggcgtcg gaggccatcg
      301 cgcaaccggc cagcaggtcg tcggcctcgt caccatgggc gtcgcggcac ttctcgatcg
      361 cgatccggct ggagtcgacc cggctcatct gaccggctcc gatccccagg gtcaccccgt
      421 ccttggcgaa gacgatcgcg ttcgacttga catggcgaca gacggtccag gcgaagagca
      481 tgtcgatcca ggcctgctcg gaaggctgcg ccttggtgac cacttccatc atctcccgct
      541 gttcgacctc cccgtcgcgg tcctggatca ggatgccgcc gcggacccgc ttgatgtcct
      601 tctcgcgcga gtcggggaag ggcggggcct cgtcggtgaa gatgcggatg ttctccttct
      661 gctggaggat ctccatcgct tccggctcgt atccgggggc gacaagcacc tcgacgaagt
      721 tcttgtgaag ggcctcggcc agcggcacgt cgatcacctg attgattgcg atcaccccgc
      781 cgaaggcgga gaccgggtcg cagtcgcggg cccgttcgta ggcgtcgagc ccggagtcac
      841 ccagggcgac cccgcagggg ttgttgtgct tgacgatcac gcaggccggg ccttcgaggt
      901 cggccagcag cttgcggcaa gcgtcgaggt cgagcacgtt gttgaaggag agcgccttgc
      961 cgtggtgctt cgacatcccg gtcagcacgt tgctgtcaag gccgacctgc gagtagagcg
     1021 cggcccgctg gtgcgggttc tcgccgtagg aaaggtcgag ctgcttctcg aaggccagca
     1081 cgcggtgctc ggggaaggct tcgtaggact cggaaaacca ggccgagatc gcggcgtcgt
     1141 aacgggcggt ctgggcgaag gcttcattgg cgagccagtg ccgggtcgag gcggagacct
     1201 cgccttcggt ctggttcatc tcctcgatca ccgcgtcgta acactcgggc ttgaccagca
     1261 ccaccacgtc ctcgtggttc ttggccgcgg cgcggatcat ggtcgggcca ccgatgtcga
     1321 tgttttcaat cgcctcctgc gggccgacgt cctcacgcgc gatcgtctgc tcgaaggggt
     1381 agaggttgac gcagaccagg tcgatcggcg ggatctcctc ctcggtcagg acctccatgt
     1441 gggccgggtc cgagcggcgg gcgagcagtc cggcgtagat cttcgggtgg agagtcttga
     1501 cccggccgtc catgatttcg cgctggccgg tgaactcgga aacgtcgatc acggtcagtc
     1561 cggcgtcgcg cagggcccgg gcggtgccgc cggtggagag gatctcgacc ccgagatcgg
     1621 ccaacccctt ggcgaagtcg gcaagcccgg tcttgtccga gaccgagatc agggcgcgcc
     1681 tgacctttac cggtccgttg gtcggatcca tgttgtccat atcaaccctc ttgttcgttt
     1741 cgaagcgccc ggggacggcc ggcgtcactt cccacgtgtt tgcgtgacga tcaccttgcg
     1801 aggttcgtca tcggcgatct taacttcgcc ccgggcaatc atgccgatcg cctcggggta
     1861 gatctcatgc tcgatcgcgt ggatcgccac ttcgagttcc tcacggtccc aatttgcggg
     1921 aacttccacc gcccgctgga tgatcaccgg cccggtgtcg gttccttcgt cgacgaagtg
     1981 gacggtcacc ccggtcacct tcacgccctg ctcgagcgcc tgcccgatcg cgtcaagtcc
     2041 ggggaacgag ggaagcagag ccgggtggac gttgacgatc tgcccccgga accgctcgac
     2101 gaaggacgcc gagaccagct gcatgtatcc ggccagggca accagttcgg ccccggtcga
     2161 ctcgatccag tcggccatcg ccgcatcccg ggcctcccgg tcctcgtact cgtcccgggg
     2221 aaagaccgcg gtcggcacct tcgccgcggt cgcccgttcc agcgcatagg ccgccggctt
     2281 gtcggaacca accccgacca cctcgacctc gccccgcccg tgcagctggt caagaacagc
     2341 ctgaaggttg gtccccccac cggaggccag caccacaacg cgaaacggag aagacggggt
     2401 cacggcgcca tcttccctga tctcgcccga tgccacccgt taggttgaag ccatgggatt
     2461 cacgcaggga acccacagaa acacgaggcg gacggtttgg ctggccgttg cgggattgat
     2521 tctgtccctt ctgctggtcg ccgcggtttg tccttccccg gcggaagcca tccagtactt
     2581 cggcaagaag cccggcaagt gggaagccga gatcgagatc cggaacggtt tcatccggtc
     2641 catgcacctg aagcttccgt cgaactgccg cgaccaggag cgcccgccgg gcctggacct
     2701 ggagatcaac cgggattcga agctccggct ccgccggtac ggcggcttct accgcgagtc
     2761 ggtgacgcgc tggagcaagt cggtcctgaa gggccgggtg atcggcaaca gggtccaggg
     2821 atccctctgg tttgcggacc tcggtgatta cgtccaatgc tggaccggaa ccggggagaa
     2881 gccctcctgg gtcaggttcg ccgcccgggc cgtc
//
LOCUS       DAOQMF010000168         2899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19681_length_2899_cov_2.404712, whole genome shotgun sequence.
ACCESSION   DAOQMF010000168 DAOQMF010000000
VERSION     DAOQMF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19681_length_2899_cov_2.404712"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1627
                     /gene="murD"
                     /locus_tag="PLV77_03805"
     CDS             260..1627
                     /gene="murD"
                     /locus_tag="PLV77_03805"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNC15001.1"
                     /translation="MEYVRSSKARPPLPQGPFLVVGLARSGVAAARALKAHGEAVFGV
                     DSGEPEGLELLRESGIDFETSVPGTDRLDGVATVIKSPGVPESAEVIDEASRRGLPVI
                     GELELGWRMFEAPVVAITGTNGKTTTTELTAHLFRSAGRPVSAAGNVGHPVCEVAMEA
                     AAGREVGTVVCECSSFQLEDSVEFSPEVAVFLNLGPDHLDRHGDMGSYLEAKLRIFAN
                     QVEGDVAVLNADDPQVAVSETTAAKIRFSTAGAEGAELTLDGETILVDGEPLIEVSEM
                     QIIGRHNVANAMAGAASAIALGLSTDEVAEGLKSFPPVPHRYEPIAEIEEVVFINDSK
                     ATNVDATLAAIDSSDRTLHLILGGSTKGEPFDELVGPVAAHCRGIYLIGVTANEIGKA
                     LEPAGVEIVRYCEDLEAAVSEAAGAATPGEAVLLSPACASFDQFKNYEDRGDSFRRFV
                     EDLDG"
     gene            1620..>2899
                     /locus_tag="PLV77_03810"
     CDS             1620..>2899
                     /locus_tag="PLV77_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNC15002.1"
                     /translation="MAERRRKKTSRKSKPSTRGSALRPAKVGKQGFSTEYSLMLTATM
                     ILLAFGVVMVFSASSTSQILSDGSLAGSTFYLKKTLIAVTIGLICMWLVMRGSLSRFR
                     EMTPKFMIGTLVALVLVLFMGAAINGTRGWFIFGPVQIQPAEFVKLGLILYGANLLAD
                     RPDRLYSVRDMGPYLGMTGVALLLVLAQPDMGSAMVAVFAVFVTLFAAGARPRDLGLL
                     AGGVGGIAFLFALAAPYRRDRLLTFLNPDGDVTGNGFQIIQAKIAIGSGGFDGVGIGN
                     GVQKAFYLPEAHTDMISAVIGEEFGFLGMFALILVYGMLGYAGFQIARKAKDDYGRIL
                     AGGLTGLILIQACINLYAVMGMAPLTGVPLPLVSYGNNSLIVSLISIGLILNVGRGGR
                     AATLSKPVPRRGPRPNARLRLIEGEGQRKPTQPA"
BASE COUNT          507 a          940 c          931 g          521 t
ORIGIN      
        1 cacatacccg cctcccgaga acgagggttc aaacccggcc ctggaggtgt cgcgggactc
       61 ggggccacct tcaaatcccc cgtcacctgc ccggttcaac ccaagtgggt cggattctcg
      121 gcagtcgggc cggaaatccg acccacctga actcatggtg taaccgggcc tgtcccgggc
      181 cgatttcgtg gccgatgaac cgggcgaaag aattcggctt tggggcaggt gatcggcttc
      241 ccgtggggaa gactcctgag tggagtatgt ccgctcatcg aaggcccgcc cgccgcttcc
      301 ccaagggcct ttcctcgtag tcgggctggc acgctccggg gttgccgccg ccagggcgct
      361 gaaagcccac ggtgaagccg ttttcggggt tgactccggc gagcccgaag gccttgagct
      421 gctgcgtgag tcaggcatcg actttgagac ctccgtcccg gggaccgatc ggctcgacgg
      481 ggttgccaca gtgatcaaga gcccgggcgt gcccgagtcg gccgaagtga tcgacgaagc
      541 gtcccggcgc gggctgccgg tgatcggtga actcgagctc ggctggcgca tgttcgaagc
      601 accggtcgtc gcaatcaccg ggaccaacgg caagacgacc acgaccgagt tgaccgccca
      661 ccttttccgc tcggccggcc ggccggtgtc tgccgccggc aacgtcgggc atccggtctg
      721 cgaggtagcg atggaagcgg cggctggacg ggaggtcggc accgtggtct gcgaatgctc
      781 cagcttccag ctcgaggatt cggtcgagtt ctcgcccgag gtcgcggtgt tcctcaatct
      841 gggtccggat catctcgacc gccacggcga catgggctca taccttgaag cgaagctcag
      901 gatcttcgcc aatcaggtcg aaggcgatgt cgccgtattg aacgccgatg acccccaggt
      961 tgccgtcagc gagacgaccg cagcgaagat ccgcttctcg acggccggtg ctgaaggagc
     1021 ggaactgacc ctggacggtg aaaccatcct ggttgatggc gaaccgctga tcgaggtcag
     1081 cgagatgcag ataatcggcc gccacaatgt cgccaacgcg atggccggtg ccgcctccgc
     1141 aatcgcgctc gggctcagca cggacgaggt cgccgaggga ctgaagagct ttccgccggt
     1201 gccgcatcgc tacgagccga tcgccgagat cgaagaggtt gtattcatca acgattccaa
     1261 ggcgaccaac gtcgacgcga ccctggccgc catcgattcc tccgaccgaa cccttcacct
     1321 gatccttggc ggcagcacca agggtgagcc cttcgacgaa ctggtcggac cggtcgccgc
     1381 acactgccgt ggcatctacc tgatcggcgt gaccgccaac gagatcggca aggccctgga
     1441 gccggccgga gtcgaaatcg tccgctactg cgaggatctc gaggcggcgg tttcggaagc
     1501 cgccggtgcg gcgactccgg gcgaggcggt gttgctttcg ccggcctgcg cgagcttcga
     1561 ccagttcaaa aactatgagg accggggcga ctcattccgc cgcttcgtcg aggatctcga
     1621 tggctgaacg gcgccgcaag aagacctcac gaaagagcaa gccttcgacc cgaggcagtg
     1681 cgctccggcc ggccaaggtg ggcaagcagg ggttctcgac cgagtacagc ctgatgctga
     1741 ccgcgacgat gatcctgctc gccttcggcg tggtcatggt cttctcggcc agctcgacca
     1801 gccagatcct cagcgacggc agcctcgcgg gcagcacctt ctacctcaag aagaccctga
     1861 tcgcggtgac gatcggcctg atctgcatgt ggctggtgat gcgcggcagc ctcagtcgct
     1921 ttcgtgaaat gaccccgaag ttcatgatcg gcaccctggt cgccttggtg cttgtccttt
     1981 tcatgggggc ggcgatcaac ggcacccgcg gctggttcat cttcggtccg gtccagatcc
     2041 agcccgccga attcgtgaag ctggggctga tcctctacgg agccaacctg ctggccgacc
     2101 ggcccgaccg tctctactcg gtgcgcgaca tgggtccgta cctcgggatg accggtgtcg
     2161 cgttgctgct ggtcctggcc cagccggaca tgggctcggc catggtcgcc gtcttcgcgg
     2221 tcttcgtcac cctcttcgcg gccggggcgc ggccccgtga cctcgggctg ctggccggcg
     2281 gggtgggagg gatcgccttc ctcttcgcgc tggcggcgcc ataccgccgt gaccgcctgc
     2341 tgacttttct caaccctgat ggtgatgtga ccggtaacgg attccagatc atccaggcaa
     2401 agatcgcaat cggctccgga ggcttcgacg gtgtcgggat cggaaacgga gtccagaaag
     2461 ccttctacct tccggaagcc cacaccgaca tgatttcggc agtgatcggc gaggaattcg
     2521 gtttcctcgg catgttcgcc ctgatcctgg tctacggaat gctcggttac gcgggattcc
     2581 agattgcccg caaggcgaaa gacgactacg gacggatcct cgccggcggc ctgaccggac
     2641 tgattctgat ccaggcctgc atcaaccttt acgcagtgat gggaatggcc ccgctcaccg
     2701 gcgtcccgct gcccctcgtc tcttacggca acaacagcct gatcgtcagc ctgatctcga
     2761 tcggcctgat cctcaacgtg ggtcggggcg gccgggccgc gaccctaagc aagcccgttc
     2821 cgcggagggg tccgcgaccg aatgcgagac tgcgcctgat cgagggtgaa ggccagagga
     2881 aaccaacaca accagcaag
//
LOCUS       DAOQMF010000169         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19710_length_2896_cov_4.351989, whole genome shotgun sequence.
ACCESSION   DAOQMF010000169 DAOQMF010000000
VERSION     DAOQMF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19710_length_2896_cov_4.351989"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLV77_03815"
     CDS             <1..1028
                     /locus_tag="PLV77_03815"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC15003.1"
                     /translation="GYYRYDKGFSPVFEGEEDFEGELFHAQHWPEGLDPRGRRFVVIG
                     SGATAITVVPALVEAGADVTLLQRSPTYVLSIPTTGGIGGWIRSRFSPRRAAFLLRWF
                     GILMEIVNFNLARRYPNFFRRLFISGARKQLPPDIERRHLNPRYDPWTQRVCFAPDGD
                     YFAALSGDSARIETGNIERISSDRIHLREGGEIEADVIVKATGLELLLFGDIAMTVDG
                     EPVNPAERLVYKGAMLSGIPNFVFVVGYTNASWTLKSELIARFASRFIGEVDRAGAAS
                     GTPVAPEIPMKTVPLLNLDSGYILRARDRLPLAGDTSPWVLHMNYFSDRRELLGADMH
                     DGALRYS"
     gene            1123..1644
                     /locus_tag="PLV77_03820"
     CDS             1123..1644
                     /locus_tag="PLV77_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC15004.1"
                     /translation="MKVGHRIRWSHPTGRRIRIGLAALALVAVGGAGTAIAISATSDP
                     PAPKYNSLARVVNPIGGKGRALNLARVVIQPGAKIPLHYHQGTQVGYIASGVLTYHVQ
                     SGQAKLMSGAGDAPKLVRLIKAGQTAKVKPGQWLIEQPSDHHRAANNGTKPVVILLSN
                     LLLKDAAPSRLIP"
     gene            complement(1647..2039)
                     /gene="mscL"
                     /locus_tag="PLV77_03825"
     CDS             complement(1647..2039)
                     /gene="mscL"
                     /locus_tag="PLV77_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNC15005.1"
                     /translation="MIQGYREFITRGNVIDLAVAVVIGAAFTALVNSVVADLLTPIIA
                     AIIGEPDFSALSFTIRGSEFTYGNFINALIAFLSITAVIYFFVVLPLNHLKEAEEETT
                     RECPECLSEIPNKATRCAHCTAEVTPTA"
     gene            complement(2098..2610)
                     /locus_tag="PLV77_03830"
     CDS             complement(2098..2610)
                     /locus_tag="PLV77_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15006.1"
                     /translation="MINRPPENRPGETRLLDGGFRAGLALFTCLFAFWFMFAADDAGA
                     AAYKGKTKGGSSITFALKGNRVTGIRTVVPTLCLETTGGYTSRAGSELFQPVAAGRIG
                     RTVKSKALQPAAMNVGAKATKNYEVTVNRRGRTVSGKLKLNFSFLIPDLYGARIYICS
                     GVTTFSATPR"
     gene            complement(2637..>2896)
                     /locus_tag="PLV77_03835"
     CDS             complement(2637..>2896)
                     /locus_tag="PLV77_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15007.1"
                     /translation="TAAVTASGANAPTVKVCATVSGKARNVIKPAACRTLRNVTRGST
                     VRTSLVIRTAKKARGSYPVRVAASGPGLTPVNSNNKVQVTK"
BASE COUNT          481 a          945 c          948 g          522 t
ORIGIN      
        1 ccggttatta ccggtacgac aagggtttct cgccggtctt cgagggcgaa gaggactttg
       61 aaggagagct cttccacgcc cagcactggc ccgagggact cgacccccgg ggccgccggt
      121 tcgtggtgat cggaagtggc gcgaccgcga tcaccgtcgt gccggcgctg gtcgaagccg
      181 gagccgatgt gaccctgctc cagcgctctc ccacttacgt gctttcgatc ccgaccaccg
      241 gcggcatcgg gggctggatc cgtagccgtt tctcgccgcg gcgggcggcc ttcctgctcc
      301 gctggttcgg gatcctgatg gagatcgtca acttcaatct cgcccgccgt tatccgaact
      361 tcttccgccg gcttttcatc agcggcgccc gcaagcagct gccgcccgac atcgagcggc
      421 gccatctcaa tccccgctac gacccctgga cccagcgagt ctgtttcgcc cccgacggcg
      481 attacttcgc cgccctttcc ggcgatagtg cccggatcga aaccgggaac atcgagcgga
      541 tcagctccga ccggatccac ctccgcgagg gcggcgagat cgaggccgac gtgatcgtca
      601 aggcgaccgg gctggaactc ctgctgttcg gcgatatcgc catgactgtc gacggcgaac
      661 ccgtcaatcc cgccgaacgg ctggtctaca agggagcgat gctctccggg attcccaatt
      721 tcgtcttcgt ggtcggttac acgaacgcct cctggaccct gaagtcggag ctgatcgccc
      781 ggttcgccag ccgtttcatc ggcgaggtcg atcgcgcagg cgccgcaagc ggcacgccgg
      841 tcgctccgga gatcccgatg aagaccgtgc ctttgctcaa cctcgattcc ggttacatac
      901 tgcgggcccg ggaccgcctt ccgctggccg gagacacctc cccctgggtg cttcacatga
      961 actacttctc ggaccggcgt gaacttctcg gtgccgacat gcatgacggc gcactcaggt
     1021 actcctgagt cgttcgcagc tttttccctg caaattcacg aataattcgc aggaaatagg
     1081 attggggctc cgtaacttca aggcatggga gcgaatcacc caatgaaggt tggacacagg
     1141 atccgctgga gtcacccgac cggtcgcagg atcaggatcg gcctggccgc gttggccctg
     1201 gttgccgttg gcggtgccgg caccgcgatc gcgatctcgg ccacctcgga tccgcccgcc
     1261 ccgaagtaca actcgctggc ccgggtcgtg aacccgatcg gcggcaaggg ccgggccctc
     1321 aacctcgccc gggtggtgat ccagcccggc gcaaagattc cgctccacta tcaccaggga
     1381 acccaggtcg gctacatcgc ttccggtgtc ctcacctacc acgtccagtc gggccaggcg
     1441 aagctgatgt caggcgccgg agacgcgccg aagctggtcc ggctgatcaa ggccggccag
     1501 accgcgaagg tcaagcccgg ccagtggctg atcgaacagc cctccgacca ccaccgggcc
     1561 gccaacaacg gcacaaagcc cgtggtgatc ctgctctcca acctcctcct caaggacgcc
     1621 gccccctcca ggctgatccc ctgaattcag gcggtcgggg ttacctcggc tgtgcagtgg
     1681 gcgcagcggg ttgccttgtt cgggatttcc gagaggcatt ccgggcattc ccgggtcgtt
     1741 tcctcttccg cttccttgag gtgattgagc ggcaggacca cgaagaagta gatgaccgcg
     1801 gtgatcgaga ggaaggcgat cagggcattg atgaagttgc cgtaggtgaa ctcggaaccc
     1861 ctgatcgtga agctgagcgc cgagaagtcc ggctcgccga tgatcgcggc gatgatcggg
     1921 gtcagcaggt ccgcgacgac cgagttgacc agggcagtga acgccgcgcc gatgaccacg
     1981 gcaacggcca ggtcgatcac gttgccgcga gtgatgaact cgcggtagcc ctgaatcatc
     2041 ttcacgggcc gaaactaccg ggcggccccg acgcaaagcc ggggccgccc ggccggttca
     2101 gcggggagtg gcgctgaagg ttgtgactcc ggagcagatg tagatccggg ccccgtagag
     2161 gtcggggatc aggaaggaga agttgagctt caacttgccc gagacggtgc ggccgcgcct
     2221 gttcaccgtg acctcgtagt tcttggtcgc cttggcgcca acgttcatcg cggccggctg
     2281 gagcgccttg ctcttcaccg tccggccgat ccgccccgcc gcgaccggct gaaacagctc
     2341 gctgccggcc cgggaggtgt atccgcccgt ggtctccagg cagagggtcg ggaccacggt
     2401 gcggatgccg gtcacccggt tgcccttgag ggcgaaggtg atcgagctgc cgcccttggt
     2461 cttgcccttg taagccgcgg cgccggcatc gtcggccgcg aacatgaacc agaaggcgaa
     2521 cagacaggtg aagagggcca ggccggcacg gaaaccgccg tcgagcaggc gggtttcacc
     2581 ggggcggttt tccggcgggc gattgatcat ttcgggggtg ctgtggtttc gggtgttcac
     2641 ttggtcacct ggaccttgtt gttggagttg actggggtga ggccggggcc ggaagcggca
     2701 acccggaccg ggtagctgcc acgggccttc ttcgcggtgc ggatgaccag ggaagtcctg
     2761 acggtgctgc ccctggtgac gttgcggagc gtcctgcagg cggccggctt gatcacgttg
     2821 cgggccttgc cgctgaccgt ggcgcagacc ttgacggtcg gcgcgttcgc cccgctggcg
     2881 gtaaccgccg cggtca
//
LOCUS       DAOQMF010000170         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19713_length_2896_cov_3.832453, whole genome shotgun sequence.
ACCESSION   DAOQMF010000170 DAOQMF010000000
VERSION     DAOQMF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19713_length_2896_cov_3.832453"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2344)
                     /gene="uvrA"
                     /locus_tag="PLV77_03840"
     CDS             complement(<1..2344)
                     /gene="uvrA"
                     /locus_tag="PLV77_03840"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNC15008.1"
                     /translation="MSRSERILITGAREHNLKGIDLDLPRDALIVVTGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGLMEKPDVDSIEGLSPAISIDQKTTSRNPRSTVG
                     TVTEIYDYLRLLWARIGHPHCPNCGKPITGQTQEQITDQLMTMEEGKRFMVMAPVVRG
                     RKGEYGKLFDQFRADGYSRVEVDGEMRMLDQEIVLDKKYKHDISVVVDRLVMKPDLRR
                     RLSESVEAAASLAEGLVEIEVIDGERQTFSESFACLECGTSMPELEPRIFSFNAPHGA
                     CPACTGLGFKKVIDPELVVPDPTLSVAEGALQPWRSGYSGYWRRLNKSVAETYGIDIN
                     TPWQELTQKERDVFLYGTGTERHTIRTKSRAGRQRSYQVKFEGLVNNLERRFQESDSE
                     AVREKIEDYMAEQPCPSCHGARLRPESLAVTVGGLSIRDFTDMSATAAREWIGNLDLT
                     ATERAIARLIVREVEERLSFLVNVGIGYLSLARSARTLSGGEAQRIRLATQIGSSLVG
                     VMYVLDEPSIGLHQRDNEKLIATLERLRDLGNTVIVVEHDEGTMRAADYLVDLGPGAG
                     EHGGSVVAAGTPVEVSANPDSLTGAYLVGSEQIAVPESRRSWEKALTVKGARQHNLTG
                     VDVEFPLGVLTCVTGVSGSGKSTLVNETLFPAVANRLHKAKLRPGPHDRIDGMEEIDK
                     IINIDQSPIGRTPRSNPATYTGVFDQIRQLFAATQESRARGYKPGRFSFNVKGGRCEV
                     CKGDGQIKIEMHFLPDVYVPCEQCHGHRYNRETLEVRFKGK"
     gene            complement(2417..2890)
                     /locus_tag="PLV77_03845"
     CDS             complement(2417..2890)
                     /locus_tag="PLV77_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15009.1"
                     /translation="MEREHLVRTDFPRAHGSEGFDIDSVGAHLAAVAAHVAALEARIT
                     ALQVECDTLRTQLADARDHEDGPSGLPDPAGPDPLQKLGAEVEQAPVETMPADPEPDP
                     EAGAAPGEEEAAARMLASKLAMGGTSRDTIVMQVAAQHEIDSPGALVDDVLARLA"
BASE COUNT          450 a          947 c          964 g          535 t
ORIGIN      
        1 acttgccctt gaaccggacc tccagggttt cccggttgta acggtggccg tggcattgct
       61 cgcagggcac gtacacatcg ggcaggaagt gcatctcgat cttgatctga ccgtcgccct
      121 tgcagacctc gcagcggccg cccttgacgt tgaagctgaa ccggcccggc ttgtagccgc
      181 gggcccggga ttcctgggtg gccgcgaaaa gctgccggat ctggtcgaag accccggtgt
      241 aggtggccgg gttcgagcgc ggcgtgcggc cgatcggcga ctggtcgatg ttgatgatct
      301 tgtcgatctc ctccatcccg tcgatgcggt catgcggccc gggcctcagc tttgccttgt
      361 ggaggcggtt ggccacggcc gggaacagcg tctcgttgac cagggtcgat ttgccggaac
      421 cagaaacgcc ggtcacgcag gtcaggaccc cgagcgggaa ctcgacgtcc accccggtca
      481 ggttgtgctg gcgggctccc ttcacggtca gggccttttc ccagctgcgc cggctctccg
      541 gcaccgcgat ctgctcggag ccgaccaggt aggcgccggt cagtgaatcg gggttggcgg
      601 aaacctcgac cggcgtgccg gccgcgacca cgctgccacc gtgttcgccg gccccgggcc
      661 cgaggtcgac caggtagtcg gccgcccgca tcgtgccttc gtcgtgctcg accacgatca
      721 ccgtgttgcc gaggtcacgg agcctttcca gggtcgcgat cagcttttcg ttgtcacgtt
      781 gatgaaggcc gatcgagggt tcgtccagca cgtacatgac gccgaccagg ctggagccga
      841 tctgggtcgc cagccggatc cgctgggcct ccccgccgga aagggtccgg gcggaccggg
      901 ccagagagag gtagccgatc cccacgttga ccaggaagga aagccgctct tcgacctccc
      961 gcacgatcag ccgggcgatc gcccgttcgg tggcggtgag atccaggttc ccgatccatt
     1021 cgcgggccgc ggtggccgac atgtccgtga agtcccggat cgagagaccc ccgaccgtga
     1081 ccgcgaggct ttccggcctg agccgggcac cgtgacagct cgggcagggc tgctctgcca
     1141 tgtagtcctc gatcttctcc cgaaccgctt cggagtccga ttcctggaag cggcgttcga
     1201 ggttgttgac caggccttcg aacttgacct ggtagctgcg ctggcgtccg gcccggctct
     1261 tggtccggat cgtgtggcgt tctgtaccgg tgccgtagag gaacacgtcc cgttccttct
     1321 gggtcagttc ctgccagggc gtgttgatgt caatgccgta ggtctcggcg accgacttgt
     1381 tgaggcggcg ccagtatccg gagtagccgg aacgccaggg ctgcagcgcg ccttcggcca
     1441 ccgacagggt cgggtcgggc acgaccagtt ccgggtcgat caccttcttg aagccgagcc
     1501 cggtgcaggc cggacaggcg ccgtggggcg cgttgaagga gaagatgcgc ggctccagtt
     1561 ccggcatcga agtgccgcat tcgaggcagg caaaactctc cgagaaggtc tggcgctcgc
     1621 cgtcgatcac ctcgatctct accaggcctt cggccagtga cgccgctgct tcgaccgact
     1681 cggagaggcg gcggcgaagg tccggcttca tgaccagccg gtcgacgacc accgagatgt
     1741 cgtgtttgta cttcttgtcg aggacgatct cctggtccag catccgcatc tcgccgtcga
     1801 cctcgacccg cgagtatccg tcggcgcgga attgatcgaa gagcttgccg tactcgccct
     1861 tcctgccgcg aaccaccggc gccatgacca tgaaccgctt gccctcctcc atggtcatca
     1921 gctggtcggt gatctgctcc tgggtctgcc cggtgatcgg cttgccgcag ttcgggcaat
     1981 gcgggtgacc gatccgggcc cagaggaggc gcaggtagtc gtagatctcg gtcaccgtgc
     2041 cgaccgtcga acggggattc cgcgaggtcg tcttctggtc aatcgagatc gccggagaaa
     2101 gcccttcgat cgaatcgaca tcgggctttt ccatcagtcc gaggaactgc ctggcgtagg
     2161 cggaaagcga ctcgacatag cggcgctggc cctccgcata aatcgtgtcg aaggcaaggc
     2221 tggacttgcc ggaacccgac agtccggtca ccacgatcag ggcatcgcga ggcaggtcga
     2281 gatcgatgcc tttgaggttg tgctcacggg cgccggtaat caggatgcgt tcagaacggc
     2341 tcacgccccc caatattagg gaaccgaaca cacgttcgtt tgcacccgaa ccccttccat
     2401 gccgcgcagg gcgggttcag gcgagtcggg cgagcacgtc gtcgacgaga gcgccgggac
     2461 tgtcgatctc gtgctgcgcg gcgacctgca tcacgatcgt gtcgcggctg gtaccgccca
     2521 tcgcgagttt cgaggcgagc atccgggccg ccgcctcttc ctcgccgggt gcggctcccg
     2581 cctcgggatc cggttcgggg tcggccggca tcgtctcgac gggcgcctgc tcgacctcgg
     2641 cacctagctt ctgcagcggg tcaggcccgg ccggatcggg aaggccgctc ggaccgtcct
     2701 cgtgatcgcg cgcatcggca agctgggttc gcagggtgtc gcattccacc tgcagggcgg
     2761 tgatccgggc ttccagcgcg gcaacgtgcg ccgccaccgc ggccaggtgg gcgccgaccg
     2821 agtcgatgtc gaaaccttcg gacccgtggg cccgcggaaa atccgttcga accaggtgct
     2881 ctcgctccat tcagaa
//
LOCUS       DAOQMF010000171         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19754_length_2893_cov_3.129669, whole genome shotgun sequence.
ACCESSION   DAOQMF010000171 DAOQMF010000000
VERSION     DAOQMF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19754_length_2893_cov_3.129669"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /gene="gatA"
                     /locus_tag="PLV77_03850"
     CDS             <1..1377
                     /gene="gatA"
                     /locus_tag="PLV77_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNC15010.1"
                     /translation="VLAFWLDRAGRDRLNGYLWQADRDSARQGLGEGGPLDGVPLAIK
                     DIFCTEGVPTTAASRILGGYVPPYTATSVRKAMAAGMPMLGKTNMDEFAMGSSNENSA
                     FGACLNPWDTERVPGGSSGGSAAVVAGGLAPWSLGTDTGGSIRQPAGFCGIVGLKPTY
                     GAISRFGMIAFASSLDQCGPLTRNVTDAALLLNVLQGRDEMDSTSVGIEGGIKAPDAE
                     RLDGMTFGVPRALMGEGIEAGVLARFEDTLIRIAELGGEIVDIELPHAGHAISAYYVL
                     APAEASTNLSRYDGVRYGLRASNEDLLSMYEVTREDGFGPEVKRRIMLGTYALSSGYY
                     DAYYGRAQRVRTRIVEDFEKAFGEVDFVVTPTSPTVAFKLGEKTSDPWTMYLSDVFTV
                     PISLAGVPAISIPAGLAEPDGGGSELPVGFQIAAPAFAEQKLLEASYALEQAIGFDGS
                     AALEGGVS"
     gene            1374..2810
                     /gene="gatB"
                     /locus_tag="PLV77_03855"
     CDS             1374..2810
                     /gene="gatB"
                     /locus_tag="PLV77_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNC15011.1"
                     /translation="MSTEYEAVIGLEIHVQLSTRTKMFCGCELSFGDDPNVHTCPVCL
                     ALPGVLPVPNAQAIEYALQIGAALGCELSPVSTFDRKNYFYPDNPKAYQITQAARPFA
                     VNGRLGEVRIHHAHLEEDAAKTIHTGGGGRIKGSDASEVDFNRGGTPLVEIVTEPDLR
                     SGAQVREWGQLLRATIKQLGVSDVNMEEGSLRCDANVSIRPVGSTEFGTKTEMKNMNS
                     FRFIERGIEAEVARQKEVVASGGQVVQETLHFDPATGELHSLRSKEEAHDYRYLPEPD
                     LVPVVPTPAMLEEAASSLPELPAARAARYEGIGVSEQAALLLSFELEWAGYFEGVLAA
                     DQSIEPRVAANWVTGEFAAELRKQDDQALADSKVGPESLATLIGLVQAKKISHGAGKT
                     VLEEMVASGTDPVTVVAEKGLEQIADSGELEKIVIQAIEDNPKPAEQVREGNEKAIGA
                     LVGAVMKATQGRADGGEVNRLLRQHLGL"
BASE COUNT          510 a          960 c          946 g          477 t
ORIGIN      
        1 gtactcgcct tctggctcga ccgggccggt cgcgaccggc tgaacggata cctctggcag
       61 gccgatcgtg attctgcccg ccagggactg ggcgaggggg gtcctctcga cggggtgccg
      121 ctcgcaatca aggacatctt ttgcaccgaa ggcgtgccga ccacggccgc ctcgaggatt
      181 ctcggcggct acgtgccgcc atacacggcc acctcggtga ggaaggccat ggcggcgggg
      241 atgccgatgc tcggcaagac caacatggac gagttcgcga tgggctcctc gaacgagaat
      301 tcggccttcg gtgcctgcct caacccctgg gacaccgaaa gggtgccggg aggatcttcg
      361 ggcgggtcgg cggcggtcgt cgccggcggg ctcgccccct ggtcgctcgg caccgacacc
      421 ggaggttcga ttcgccagcc ggccggattc tgcgggatcg tcggccttaa gcccacctac
      481 ggcgcgattt cccgcttcgg gatgatcgcc ttcgcctctt ctctcgatca atgcgggccg
      541 ctgaccagga acgtcaccga cgcggccctg ctcctgaacg tgcttcaggg gcgtgacgaa
      601 atggattcga cctcggtcgg catcgaaggc gggatcaagg cgccggacgc cgaacggctt
      661 gacggcatga ccttcggcgt gccccgggcc ctgatgggcg agggaatcga agccggtgtc
      721 ctggcccggt tcgaggacac gctgatccgg atcgccgaac tcggcggcga gatcgtcgac
      781 atcgaactgc ctcacgccgg gcacgcgatc tccgcctatt acgtgctcgc cccggccgaa
      841 gcctcgacca acctctcacg ctatgacggg gtccgttacg gcctcagggc cagcaacgaa
      901 gacctgctct cgatgtacga ggtgacccgc gaagacggct tcgggcccga ggtcaagcgg
      961 cgcatcatgc tcgggaccta cgccctttcc tcgggctact acgacgccta ttacggccgg
     1021 gcccagcggg ttcgcacccg catcgtcgaa gacttcgaga aggccttcgg tgaggtcgac
     1081 ttcgtggtca cgccgacctc gccgaccgtt gccttcaaac ttggcgagaa gacctccgac
     1141 ccctggacga tgtacctgag tgacgtgttc acggtgccga tctcgctggc cggggtgccg
     1201 gccatctcga tcccggccgg actggccgaa ccggatgggg gaggctccga actgccggtc
     1261 ggcttccaga tcgccgcccc cgccttcgcc gagcagaagc tgctcgaggc ctcctacgcg
     1321 ctggaacagg cgattggctt cgatggttcc gccgccctcg aagggggtgt ctcgtgagca
     1381 ccgaatacga agcggtgatc gggctggaga ttcacgtcca gctttcgacc agaaccaaga
     1441 tgttctgcgg ctgcgaactc tccttcggtg acgatccgaa cgtccacacc tgccccgttt
     1501 gcctggccct gcccggcgtg ctgccggtgc ccaacgccca ggcgatcgag tacgcgcttc
     1561 agatcggcgc cgctctcggc tgcgaacttt cccccgtctc cacctttgac cggaagaact
     1621 acttctatcc ggacaacccc aaggcgtacc agatcaccca ggcagccagg ccctttgcgg
     1681 tcaacggccg gctcggcgaa gtacggatcc atcacgccca cctcgaggag gatgcggcga
     1741 agacgatcca caccggaggc ggcggccgga tcaagggttc cgacgcctcg gaggtcgatt
     1801 tcaaccgggg cggaaccccg ctggtagaga tcgtcaccga accggacctt cgttccggcg
     1861 cccaggtccg ggaatggggc cagctcctga gggcgacgat caagcaactc ggcgtctccg
     1921 acgtgaacat ggaggaaggc tcgctgcgct gcgacgccaa cgtctcgatc cgcccggtcg
     1981 gcagcaccga gttcggtacc aagacggaga tgaagaacat gaactccttc cgcttcatcg
     2041 aacgcggcat cgaggccgag gtcgcccgcc agaaggaagt cgtcgcgagc ggcggccagg
     2101 tggtccagga aacacttcac ttcgatccgg cgaccgggga gcttcactcg ctccgttcca
     2161 aggaagaagc acacgactac cgctacctgc cggaacccga tctggtcccg gtcgtgccga
     2221 ccccggcgat gctggaggaa gctgcgagct cgcttcccga actgccggcc gcccgggccg
     2281 cccgctacga agggatcggc gtatcggaac aggctgccct gctgctctcg ttcgagctcg
     2341 aatgggccgg gtacttcgag ggcgtgctgg cggccgatca atcgatcgaa ccgcgggttg
     2401 ccgcgaactg ggtgaccggc gagtttgccg ccgagctgcg caaacaggat gaccaggccc
     2461 tggccgactc gaaagtcggg ccggagtccc tggctacgct gatcggcctg gttcaggcga
     2521 agaagatctc ccacggggcc ggcaagacgg tgcttgagga gatggtcgcc tccggaaccg
     2581 atccggtcac cgtggtggct gaaaagggcc tggaacagat cgccgattca ggcgagctgg
     2641 agaagatcgt cattcaggcg atcgaggaca atccgaaacc ggccgaacag gtccgggaag
     2701 gcaacgagaa ggcgatcggc gccctggtcg gagccgtgat gaaggccacc cagggccgcg
     2761 ccgacggagg cgaggtcaac cgcctcctcc gtcagcacct gggcctttag ttcacgcttc
     2821 tcagcggcgg ggcagcacga cccggaaact gcggacggcc ggttttttgc tcttgtgtcc
     2881 ggtctgatcg acc
//
LOCUS       DAOQMF010000172         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19804_length_2889_cov_3.094566, whole genome shotgun sequence.
ACCESSION   DAOQMF010000172 DAOQMF010000000
VERSION     DAOQMF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19804_length_2889_cov_3.094566"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PLV77_03860"
     CDS             <1..731
                     /locus_tag="PLV77_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15012.1"
                     /translation="EVAEAREEFSLSHGAGFVGSVFLIMFSEQVFLNAGPLVVRAVEG
                     VAAAGYIFNVLMIARAPLQLFQAVSTSILPHLTALHHSSDPNSEKEFHHSVRMVLLGI
                     AAFTALTAVVVMIAGPKLMQIAFSDKFTYDRLGLMLVTAGMGLYLAAVTVNQACLAQG
                     QARRSSIRWISCAVFFIIWTILPFISDGNLRVEIGFALTALLLLSLLTFIYFRPKGVE
                     ADVPLEGSSEAIEARLAGLEDTST"
     gene            complement(814..1494)
                     /gene="rpe"
                     /locus_tag="PLV77_03865"
     CDS             complement(814..1494)
                     /gene="rpe"
                     /locus_tag="PLV77_03865"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNC15013.1"
                     /translation="MPEAFQGIRIAPSILSADFGRLGSQIAEIMDAGARVIHFDVMDG
                     QFVPPITIGPLVLSGIADQVHDAGGVVDVHLMIEDPDRQFEDFARAGADSIIFHEEAT
                     PHAHRCCHAIRELGCLAGVAINPGTPVETVSELKGMVDEILVMTVNPGWGGQPFIETS
                     PDKVRRMRSLVGDDVLIEVDGGIGTGTAGTMAAAGATLMVAGSAIFGSDDPAQAYRDI
                     HAAAESGA"
     gene            complement(1547..1681)
                     /locus_tag="PLV77_03870"
     CDS             complement(1547..1681)
                     /locus_tag="PLV77_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15014.1"
                     /translation="MYCLSRYELVSQCPNCGEHQTIARMSTTEDMSCQSCGASMLQPI
                     "
     gene            complement(1935..>2889)
                     /locus_tag="PLV77_03875"
     CDS             complement(1935..>2889)
                     /locus_tag="PLV77_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15015.1"
                     /translation="LTEKLADDSTPGKAAIAHSIPDWLARLWWEELGPDRARALMAAS
                     NRPSERAVRVNTARTTVEEAIERLSGTDLELVPAEGPWPLAPPELLVARGSLAAIEEA
                     AQEGLVVSQARGSAAVVEVLDPLPEERILDLCAGPGIKTGQIAARVGRAGNMVSVEPD
                     EERAHEVAAQLERLGFHHGLVVEVDARHGEIMSGFDRVLVDAPCSDLGALASRPDARW
                     RKSPALIERVAELQGEILDRAAGFLREGGSLVYSTCTISKRENSDQALALAKEHGFTI
                     DDLGGRAPQLADPHDSRFLQLMPDRDHTTGFFIARFISGGD"
BASE COUNT          461 a          967 c          955 g          506 t
ORIGIN      
        1 ccgaggtcgc cgaggcccgg gaggagttct cgctttcaca cggcgccggc ttcgtcggct
       61 cggtctttct gatcatgttc agcgagcagg tctttctcaa cgccggcccg ctggtggtca
      121 gggcggtcga aggagtggcc gcggccggct acatcttcaa cgtcctgatg atcgcccggg
      181 ccccgctgca gctctttcag gccgtttcga cttcgatcct cccccacctg accgcgctgc
      241 accacagctc ggatccgaac tcggagaagg agttccacca ctcggtccgg atggtgctgc
      301 tcggaatcgc cgccttcacc gcgctgaccg cggtcgtggt gatgatcgcc ggcccgaagc
      361 tgatgcagat cgccttctcg gacaagttca cctatgaccg gctgggcctg atgctggtca
      421 ccgccggcat gggcctgtac ctggccgcgg tcacggtcaa ccaggcctgc ctcgcccagg
      481 gccaggcccg gcgttcctcg atccgctgga tctcctgtgc ggtcttcttc atcatctgga
      541 cgatcctccc gttcatcagc gatggcaacc tgcgggtcga gatcggcttc gccctgaccg
      601 ccctgctcct gctcagcctg ctgaccttca tctacttccg gccgaagggc gtcgaagcgg
      661 acgtgcctct cgaaggctca tccgaggcga tcgaggccag actcgccggc ctcgaagaca
      721 cttcgaccta ggtcgcctca atcagtcttg aagttattcg ctccatgtgc gaataacttc
      781 aacgctgatc tgagcggtcg agccccgaag gactcaggca ccggattccg cggcagcgtg
      841 aatgtcgcgg taagcctgag ccgggtcgtc ggaaccgaag atcgccgagc cggcgaccat
      901 cagggtcgcg ccggccgcgg ccatcgttcc ggcggtcccg gtgccgatgc cgccgtcaac
      961 ctcgatcagg acgtcatcac cgaccagcga gcgcattcgc ctgaccttgt ccggcgaggt
     1021 ttcgatgaag ggctggccgc cccagccggg gttcacggtc atgaccagga tctcgtcgac
     1081 cattcccttc agctcggaaa ccgtttcgac cggagtgccg gggttgatcg cgacgccggc
     1141 caggcagccc aactcgcgaa tcgcgtggca gcagcggtgg gcatgcgggg tcgcttcctc
     1201 atggaagatg atcgaatccg ccccggcccg ggcgaagtcc tcgaactgac ggtcgggatc
     1261 ctcgatcatc aggtggacgt caaccacccc gccggcgtca tggacctgat cggcgatgcc
     1321 ggaaagcacc agcgggccga tcgtgatcgg cgggacgaac tggccgtcca tcacgtcgaa
     1381 gtgaatgacc ctggcgccgg cgtccatgat ctcggcgatc tgcgagccca gccggccgaa
     1441 gtcggcggag aggatcgaag gagcgatgcg gatgccctgg aaagcttcgg gcacaggggt
     1501 ttcggagttg cggggctggt tggtcatcct gggatccggt cggggttcag attggctgga
     1561 gcatcgaggc gccgcagctc tggcagctca tgtcctcggt ggtgctcatt ctggcgatcg
     1621 tctgatgttc accgcagttc gggcactggg aaaccagttc gtagcgggaa aggcagtaca
     1681 cacagcggaa ccggccggga agctgagtcg gtcgcaacca gggttccccg cagcccggac
     1741 aggccggacg gcggtcgact gttgtcttct ctgactcggt cgccatgggg cgattatccc
     1801 ggaccggtcg gaaactcggc gcgcctcccc gttccggggg cgtttggcca ccgaaaaggt
     1861 gggtgatcgc ccccggaacg ggctggcacg aacgccaccg gacttccggg ggcgttcggc
     1921 cacggatcca gtggtcaatc gcccccggaa atgaagcggg cgataaagaa gccggtcgtg
     1981 tggtcgcgat ccggcatcag ctggaggaag cgggagtcat gcgggtcggc cagctgcggg
     2041 gcgcgcccac cgagatcatc gatcgtgaag ccatgctcct tcgccagggc aagggcctga
     2101 tcgctgtttt cgcgtttcga gatcgtgcag gtcgagtaga cgagacttcc gccctcgcgg
     2161 agaaagccgg ccgctcgatc gagaatctcg ccctggagct ccgccaccct ttcgatcagg
     2221 gccggggact tccgccagcg ggcatccggc cgggaagcga gcgcgccgag gtcggagcac
     2281 ggtgcgtcga ccagaacccg gtcgaagccg ctcatgatct cgccgtgcct ggcgtccact
     2341 tcgaccacga gcccgtgatg gaagccgagg cgctcgagct gggccgcgac ctcatgggcc
     2401 cgctcttcat ccggctcgac cgacaccatg ttcccggccc gccccacccg ggcggcgatc
     2461 tgaccggtct tgatcccggg gcccgcacag aggtcgagga tgcgttcctc cggcagcgga
     2521 tccagcacct cgaccacggc cgccgagccg cgggcctggg aaacgacgag tccctcctgc
     2581 gcggcttcct cgatcgcggc aaggctgccc cgggcgacca acaattccgg tggcgcgagt
     2641 ggccagggcc cttcggccgg gaccagctcc aggtcggttc cggagagccg ttcgatcgct
     2701 tcctcgaccg tggtccgggc cgtgtttacc cgaaccgccc gttcggaggg ccggttcgag
     2761 gctgccatca gggcgcgggc ccggtcgggt ccgagttcct cccaccagag ccgtgccagc
     2821 cagtccggga tcgagtgggc gatcgccgcc ttcccggggg ttgaatcatc agccagtttc
     2881 tccgtcagg
//
LOCUS       DAOQMF010000173         2884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19859_length_2884_cov_3.102510, whole genome shotgun sequence.
ACCESSION   DAOQMF010000173 DAOQMF010000000
VERSION     DAOQMF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19859_length_2884_cov_3.102510"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLV77_03880"
     CDS             <1..583
                     /locus_tag="PLV77_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC15016.1"
                     /translation="EDFLLERPLMKAIANPDPVVLLIDEIDKTDQEFEAMLLELLSDF
                     QITIPEMGQISATTHPIVLLTSNNSRELTEALKRRCLYLWLDYPARDREIEIIRLHAP
                     EIDEALAGRLVDVVDMVRELDLKKPPSIAEAIDWARTLILMGADDIDEKVFRESLSII
                     IKHRTDLDLVAEKIVPRLGGSGSSNGSSANGAG"
     gene            599..2029
                     /locus_tag="PLV77_03885"
     CDS             599..2029
                     /locus_tag="PLV77_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC15017.1"
                     /translation="MSETPSGMAEQILAFCEELRSEDVAIGTSEIQDAFRALEIVPWT
                     EPVDFRESLATTLAKSPKDREIFDLVFDRHFFRATEAASLEFAPEGGEGRKAALSDEV
                     REELSMEALAEAIRQAIAEGDESAMNELARLAIEAFGRQGESSGVVGVDMQRIRRGLG
                     LSPSSDTSGDGEADGENLDRDQIARFERQLRRELQRQQIQKQGQLPPARPLSDLNRAL
                     PMRGAQDLAAVHKAVAQMKRRLATLGHDPRGAKRGRTVDMRRTMRSSLETGGVPLNLK
                     YRPRRPRKPEIYVLCDVSTSVSSASTFFLSVLHALHDSFRKLRSFVFIERISEVTHVF
                     EDERDFTAIAEKITATGGVADISGYTDYGRVWNEFYADVSADLGPRSTVIVLGDARTN
                     GRPPAEDAFARVADRANRTFWLNPEPELYWNYGDSVMSAYTPFCDGAFECWKTEHLEQ
                     FVDVVASGKVEHFGTTTRKYTSGHRY"
     gene            2033..2323
                     /locus_tag="PLV77_03890"
     CDS             2033..2323
                     /locus_tag="PLV77_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNC15018.1"
                     /translation="MTAGAGGFSRRIRVRLQPRASKNELKGWKEDPGSGEQVLQARVT
                     APPVDGKANQALIALLAKEFKTPKTRIRIIQGESARDKVVELAESDRDGHRP"
     gene            complement(2320..>2884)
                     /locus_tag="PLV77_03895"
     CDS             complement(2320..>2884)
                     /locus_tag="PLV77_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNC15019.1"
                     /translation="LPGDPFGLDADGDGMACADLPCPCSKAAGGGSGSGDGSAADGST
                     GSEPPRRFKGTVVDVADGDTLDVSTPSGTVETVRVIGIDTPEVYYGTECGGPAASGAM
                     KRLATGQQVTVTSDPTQDRRDHYGRLLAYIDRGSQDLGYTLVKRGLASAYPYDGPFQR
                     YPRYARANNHARTNNIGSWSHCDISPG"
BASE COUNT          502 a          953 c          976 g          453 t
ORIGIN      
        1 cgaggatttc ctgctcgagc ggccgctgat gaaggcgatc gccaacccgg acccggtggt
       61 cctgctgatc gacgagatcg acaagaccga ccaggagttc gaggcgatgc tgctcgagct
      121 gctttccgat ttccagatca cgattccgga gatggggcag atcagcgcca cgacccaccc
      181 gatcgtgctg ctgacttcga acaattcgcg cgagctgacc gaggccctga aacggcgctg
      241 cctctacctc tggctcgatt atccggcccg ggatcgggag atcgagatca tccggcttca
      301 cgcgccggaa atcgacgagg ccctggccgg ccggctggtt gacgtggtcg acatggtgcg
      361 cgagctggat ctgaagaagc cgccttcgat tgccgaggcg atcgactggg cccggaccct
      421 gatcctgatg ggcgccgacg acatcgacga gaaggtcttt cgcgagtcgc tttcgatcat
      481 catcaaacac cgcaccgacc tcgatctggt cgccgagaag atcgtgccac ggctcggcgg
      541 ttccggatcg tccaacggca gctccgccaa cggcgccggc taggacggga gccatccgat
      601 gagcgagacc ccttcgggaa tggcggagca gatcctcgcc ttctgcgagg agcttcgttc
      661 ggaggatgtc gcgatcggca cctcggaaat ccaggacgcc ttccgggcgc tggagatcgt
      721 gccctggacc gagccggtcg acttccggga atcgctggcc accacgctgg ccaagtcgcc
      781 gaaggaccgc gagattttcg acctcgtctt cgaccggcat ttcttccggg cgactgaagc
      841 cgcctcgctc gagttcgcgc cggagggtgg cgagggccgc aaggccgcgc tctccgacga
      901 ggtccgcgag gaactgagca tggaggccct ggccgaggcg attcgccagg cgatcgccga
      961 gggcgacgaa tcggcgatga acgaactggc ccggctggcg atcgaggcct tcggccgcca
     1021 gggtgaaagc tccggggtgg tcggggtcga catgcagcgg atccgccgcg ggctcggcct
     1081 ctccccctca tccgacacaa gcggtgacgg cgaagccgat ggcgagaacc ttgaccggga
     1141 ccagatcgca cgcttcgaac gccagctccg gcgcgaactc cagcgccagc agatccagaa
     1201 gcagggccag ctgcccccgg cccggccgct ctcggacctg aaccgggccc tgccgatgcg
     1261 cggcgcccag gacctcgcgg ccgtgcacaa ggcggtcgcc cagatgaagc gccgcctggc
     1321 cacgctcggc cacgacccgc gcggggcgaa gcgcggccgg acggttgaca tgcggcgaac
     1381 gatgcgctcc tcgcttgaga ccggcggggt tccgctcaat ctgaagtacc gcccgcgccg
     1441 gccgcgcaag ccggagatct acgtgctctg cgacgtctcg acttcggtca gctcggcctc
     1501 gaccttcttt ctttcggtcc ttcacgcgct ccacgattcg ttccgcaagc tgcgcagctt
     1561 cgtcttcatc gaacggatct cggaggtgac gcacgtattc gaggacgaac gggatttcac
     1621 cgcgatcgcc gagaagatca ccgcgaccgg cggggtcgct gacatttccg gttacaccga
     1681 ttacgggcgg gtgtggaacg agttctacgc cgatgtttcg gccgacctcg ggccccgctc
     1741 gacggtgatc gtgctcggcg acgccaggac caacggccgt ccgccggccg aggacgcctt
     1801 cgcccgggtc gccgaccgcg ccaaccgcac cttctggctc aacccggaac cggagctcta
     1861 ctggaactac ggagattcgg tgatgagcgc ctacaccccg ttctgcgacg gcgccttcga
     1921 atgctggaag accgaacacc tcgagcagtt cgtggatgtg gtcgcctccg gcaaggtgga
     1981 gcacttcggc accaccaccc gcaagtacac ctccggccac cgctactgaa ccgtgacggc
     2041 cggagccgga ggattcagcc ggcgaatccg cgtccgcctt cagcccaggg cctcgaagaa
     2101 cgagctgaag ggctggaaag aggaccccgg ttcaggcgag caggtccttc aggcccgggt
     2161 aaccgccccg ccggttgacg gcaaggccaa ccaagccctg atcgccctcc tcgccaagga
     2221 gttcaagacc ccgaaaacaa ggatccggat catccagggc gaatcggccc gcgacaaggt
     2281 ggtcgagctg gccgaatcag accgggatgg ccaccggccc taacccggtg agatgtcgca
     2341 gtgggaccag ctgccgatgt tgttggtgcg ggcgtggttg ttggctcggg cgtagcgcgg
     2401 gtagcgctgg aagggaccgt cgtagggata ggcggaggcg aggcctcgtt tgacgagggt
     2461 gtagccgagg tcctggctgc ccctgtcgat gtaggcgagg aggcggccgt aatggtcgcg
     2521 gcgatcctgg gtggggtcgg aggtgaccgt gacctgctgg ccggtggcaa ggcgtttcat
     2581 ggcaccggag gcggccggtc cgccgcattc ggtgccgtaa tagacctcgg gagtgtcgat
     2641 gccgatcacc cggacggtct cgacggtgcc ggaaggggtg ctcacgtcca gcgtgtcgcc
     2701 gtcggccacg tcaaccaccg tgcccttgaa acggcggggc ggttcgctgc cggtcgaacc
     2761 gtctgcggcg gaaccgtcac ccgaaccgga gcccccgccg gccgctttcg aacaggggca
     2821 gggcagatcg gcgcaggcca tcccgtcacc gtcggcatcg aggccgaacg ggtcaccggg
     2881 caag
//
LOCUS       DAOQMF010000174         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19888_length_2882_cov_3.121684, whole genome shotgun sequence.
ACCESSION   DAOQMF010000174 DAOQMF010000000
VERSION     DAOQMF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19888_length_2882_cov_3.121684"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLV77_03900"
     CDS             <1..198
                     /locus_tag="PLV77_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15020.1"
                     /translation="EQRERIWRLYMTASFLAFERGDVGVNQILGVIPEHEQVALYRPS
                     PGLALARPSFVREEVSAGQVG"
     gene            complement(178..>2882)
                     /locus_tag="PLV77_03905"
     CDS             complement(178..>2882)
                     /locus_tag="PLV77_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC15021.1"
                     /translation="MTPERRQPKDKTQPTQEVLLNDLRRSRSVGEAAEVTARAAVEQF
                     GAVGAVVSDRQGPIAARPTRLDPLTIAELTAIPEGVGVGSRGSVLEGMSVACFSHPEM
                     DGIRLAAAWAPESSARVDDLEAGINSFAVWLRQFADPAAERVKRSYTGPGPTATDYEQ
                     SLKRMDAEGFEHLIDSVPAILYTAEMGESGQWLYVSPQIEKILGYTPEEWLSDTTIWY
                     RSIHPDDREHALAFEDERLIGLDLHPPAEYRLKARDGRYVWIYERARLVRNEQGVPQW
                     HGVMQDISALKAAETAIELKAEQQVLVARLGEMAIRGEDPDSLLRYAVDWVGQLDGVI
                     EASIWEQEDYKELHLHHRSANLGWSNTLPYDPEQYPDSHLTQGDVVLIPSWEDDPRLE
                     RFVPFLPESVRSSMIAPIMGAVDQFGMIIVHSDRISAFSEQDGNFLRAAANVLGNSIE
                     RSRTDLSLQHRINHDPLTDLPNRQLFTRRLAEAIDEAGSREGIVALLFLDIDHFKLIN
                     DGIGHHAGDELLREIAPRLSASVRRGDTVARFGGDEFGIVLASIKSVDEAQEVARRML
                     ESISEPIPVAGTERFVTASIGVATYQPGREEPKSAEDLIRDADAAMYLAKDSGRARVA
                     SFGKPIRDRVLRRLDVERQLHGAIENEELQVVYQPIISLRTGKLRAFEALTRWDHPEE
                     GILKPELFIPIAEESDLISRIDSWVMAEAVRQAGVWNEGLDPDDRITVSVNASSRQIH
                     KSGLAAEITDLLVRCDMRPENLAIEITENVLLTGSAVIDDVLREIHELGVRLALDDFG
                     TGYSSLSYLSEFPLDAIKIDRAFVEGLGRGDPSTSAIADAIAQIGRALSLLVVAEAVS
                     TETQLQMVRDLGCSMAQGFLISPPLSVEQATDFVKGSGLPDLTR"
BASE COUNT          466 a          930 c          970 g          516 t
ORIGIN      
        1 gaacaacggg aacggatctg gcggctctac atgaccgcca gcttcctcgc cttcgaacgc
       61 ggagacgtcg gcgtcaacca gattctcggg gtgattccgg agcatgagca ggttgccctg
      121 taccggccct cgccgggact ggcgctggcc cggccctcct tcgtgcggga agaggtctca
      181 gcgggtcagg tcgggtaggc cggagccttt gacgaagtcg gtcgcctgct cgacactgag
      241 cggcggcgag atcagaaagc cctgcgccat cgagcagccg aggtcccgga ccatctgaag
      301 ctgggtttcg gtcgagactg cctccgccac gaccagcagc gaaagcgccc ggccgatctg
      361 ggcgatcgcg tcggcgatcg cagaggtgct ggggtcaccg cggccgagac cctcgacgaa
      421 ggcccggtcg atcttgatcg cgtcaagcgg aaactcgctc aggtagctga gtgacgagta
      481 gccggtgcca aaatcgtcga gcgcgagccg gacgccaagc tcgtgaattt cccgcagcac
      541 gtcgtcgatc acggcacttc cggtcagcag gacgttctcg gtgatctcga tcgccaggtt
      601 ttcgggccgc atgtcacatc tgaccagcag atcggtgatc tcggccgcga gaccggattt
      661 gtggatctgg cgcgatgaag cgttgaccga aacggtgatc cggtcgtcgg gatcgaggcc
      721 ctcgttccag acccccgcct gcctgaccgc ctcggccatc acccagctgt cgatccggga
      781 aatcaggtcg ctctcctcgg cgatcgggat gaagagctcc ggcttgagga tgccctcctc
      841 gggatgatcc cagcgggtca gcgcctcgaa ggcccgcagc ttgccggtgc gcaagctgat
      901 gatcggctgg tagacgacct gcagctcctc gttttcgatc gcgccgtgaa gctggcgctc
      961 gacgtccagc cgacgcagca cccggtcgcg aatcggtttg ccgaaggacg cgacccgggc
     1021 ccgtccggaa tccttggcca ggtacatcgc cgcatcggca tcgcggatca ggtcctcggc
     1081 cgatttcggc tcttcccggc ccggctgata ggtggcgacc ccgatgctgg cggtgacgaa
     1141 tcgctcggtc ccggcgaccg ggatcggctc cgaaatgctc tccagcatgc gacgggccac
     1201 ttcctgcgcc tcgtcgaccg acttgatcga ggcgaggacg atcccgaact cgtcgccacc
     1261 gaaccgggcc accgtgtccc cgcggcggac gctggccgaa agccgcggcg cgatctcgcg
     1321 cagcagttcg tcaccggcgt gatgcccgat cccgtcgttg atcagcttga agtggtcgat
     1381 gtcgaggaac agcagggcga cgatgccttc ccgcgagccc gcctcgtcga tcgcctcggc
     1441 cagccggcgg gtgaacagtt gccggttggg caggtcggtc agtggatcgt ggttgatccg
     1501 gtgctgaagc gaaaggtcgg tccggctgcg ttcgatcgag ttgccgagca cgttggcggc
     1561 ggcccggagg aagttgccgt cctgttcgct gaaggcgctg atccggtcgg agtggacgat
     1621 gatcatgccg aactggtcga ccgcgcccat gatcggggcg atcatcgaac tgcgaaccga
     1681 ttcgggcagg aagggaacga aacgctcaag ccgcggatcg tcctcccagg aggggatcag
     1741 gaccacgtcg ccctgggtga gatggctgtc cggatactgc tccgggtcat atggcagcgt
     1801 gttcgaccag ccgagattgg cggagcggtg gtggaggtgc agctccttgt aatcctcctg
     1861 ttcccagatc gatgcctcga tcacgccgtc aagctgaccg acccagtcga ccgcgtagcg
     1921 gagcagggaa tccgggtctt cgccgcggat cgccatctcg cccagccgcg cgaccagaac
     1981 ctgctgttcg gccttgagtt cgatcgcggt ctcggccgcc ttgagtgccg agatgtcctg
     2041 catcacccca tgccactgcg gcactccctg ctcgttgcgg accagacgag cgcgctcgta
     2101 gatccacacg taacggccgt cgcgggcctt caggcggtat tcggccgggg gatggagatc
     2161 gagtccgatc agcctctcgt cctcgaacgc gagcgcatgc tcgcggtcgt ccgggtggat
     2221 cgaccggtac cagatggtgg tgtccgaaag ccattcttcc ggggtgtagc ccaggatctt
     2281 ctcgatctgc gggcttacat aaagccactg cccggattcg cccatctcgg cggtgtagag
     2341 gatggcgggc accgagtcga tcaggtgctc gaacccttcc gcgtccattc gcttgaggct
     2401 ctgctcgtaa tcggtagcgg tcgggccggg gccggtgtag gagcgcttga cccgttcggc
     2461 ggccggatcg gcgaactgcc tgagccagac cgcgaaggag ttgattcccg cctcgagatc
     2521 gtcgacccgg gctgaactct cgggcgccca tgcggcggcc agtcgtatgc cgtccatttc
     2581 gggatgggag aagcaggcaa cggacatgcc ttcgaggacg cttccgcggg aaccgacgcc
     2641 gactccttcg ggaatcgccg taagttcggc gatggtcagc gggtcgagcc tggtcggccg
     2701 ggcggcgatc gggccctggc ggtcactgac caccgcgccg accgctccga actgttcgac
     2761 cgccgcccgg gccgtcacct cggcggcctc gccgaccgac cgtgaccggc gcaggtcatt
     2821 gaggagtacc tcctgcgtgg gctgcgtctt gtctttgggc tggcgccgtt ccggagtcat
     2881 gc
//
LOCUS       DAOQMF010000175         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19946_length_2877_cov_3.490432, whole genome shotgun sequence.
ACCESSION   DAOQMF010000175 DAOQMF010000000
VERSION     DAOQMF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19946_length_2877_cov_3.490432"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="PLV77_03910"
     CDS             <1..1747
                     /locus_tag="PLV77_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15022.1"
                     /translation="KVDALFGNPDVKLMRNNGKQDSSLITRAPTLRDIRNRGEEAYID
                     LPGDPLGETCVYAKDFDRMRREGRAPVAVYAHIAREKGHSGLALQYWFYWYFNQFNDL
                     HESDWEGMQITFDANTPAGALKQEPHEMIVFQHAGGERANWDDAKVEKEGSHPVVYPA
                     AGSHATFYQSAIYPQNGSHGSGVGCDNTSEPLTELRPVPVLLPERPTDRGEFAWLSFD
                     GRWGQKEKSFNNGPTGPQTKTQWDEPFTWMEAQRWSSPRMPGGGLVGPEAVNAFCGVI
                     ESVTGVMNLQQSDPLAAYLIAAAFILIVFFVFGYSKWRPAGADRLEQKRAYGQIMNAS
                     FRLYWRHLSAFAVLGAIAIPIVGGTQALGGWLGDMSGGDGLLQTLSDLVNSFGRPAAQ
                     ALISAFLIVFIRDLVQGQTATVRTALDGTRERFWRVVIAKMLAIAGVTLLALTVIGIP
                     FAVRFLVNWTFVQQEVIFTDLSIRDSFRASSDLVRGHWWRTVRTMVPLLLLLTVLGPL
                     LGLFLIFTPLPLLLVNLIGSVVFAIAIPFTTAGTTLLYFDLRARQQEHGVVPRRSWAV
                     WRPATFGRPVGQPTA"
     gene            1852..2535
                     /locus_tag="PLV77_03915"
     CDS             1852..2535
                     /locus_tag="PLV77_03915"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC15023.1"
                     /translation="MFDSDFDPRDRGRFEGRCGDPGFGPAAGDGQPGFGPETDSGRRR
                     YRHGPGRGGPHGFGGGHHGRGPGRPGRGRRARRGDIRQAILLLLEEEPRNGYGLMQEV
                     EERSGGAWRPSPGSVYPALSQLEDEGLVEMAESEGRKDFSLTGAGREWVEEHREEIGS
                     PWDTEGRGTPRELGSLRKSTQALAMAARQLAHTGTPAQLEEARKLVDEARRKLYGILA
                     QSEEEQEQS"
     gene            complement(2555..>2877)
                     /locus_tag="PLV77_03920"
     CDS             complement(2555..>2877)
                     /locus_tag="PLV77_03920"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HNC15024.1"
                     /translation="ATGLAFLLGAVNPKNLILTMAAATAIGQVDAGSADQLTSVLVYA
                     LIASIGAAVPVGIYFLMGDRADSMLARLKESMARNNGVIMAVICVVIAAKLIGDGITT
                     LSLF"
BASE COUNT          496 a          914 c          973 g          494 t
ORIGIN      
        1 gaaggttgac gccctcttcg gaaaccccga tgtgaagctg atgcggaaca acggcaagca
       61 ggattccagc ctgatcacca gggcaccgac cctgagagac atcaggaacc ggggcgagga
      121 ggcctacatc gacctgcccg gcgacccgct gggagagacc tgcgtgtatg cgaaggactt
      181 cgaccggatg cggcgtgaag gccgggcacc ggtcgcggtc tacgcccaca tcgcccgcga
      241 aaagggacat tccggcctgg cactccagta ctggttctac tggtacttca accagttcaa
      301 cgatctgcat gaaagcgact gggagggcat gcagatcacc tttgatgcga acacaccggc
      361 cggggcgctg aagcaggagc ctcacgagat gatcgtcttc cagcacgccg ggggagagcg
      421 ggcgaactgg gatgacgcca aggtcgaaaa ggaaggcagc cacccggtcg tctacccggc
      481 ggccggttcc catgcgacct tctaccagtc ggcgatctat ccccagaacg gctcgcatgg
      541 atcgggagtg ggctgcgaca acacctcgga gccgctgacc gagctcaggc cggtcccggt
      601 gctcttgccg gagcggccca ccgaccgcgg tgaattcgcg tggctttcct ttgacgggcg
      661 ctggggccag aaggagaaga gcttcaacaa cggcccgacc ggcccgcaga ccaagaccca
      721 gtgggatgag cccttcacct ggatggaagc gcagcgctgg tcgagccccc ggatgcccgg
      781 cggagggctg gtcggtccgg aagcggtgaa cgccttctgc ggggtgatcg agagcgtgac
      841 cggcgtgatg aatctgcagc agtccgatcc gctcgcggcc tacctgatag ccgcggcttt
      901 catcctgatc gtcttcttcg tgttcgggta ctcgaaatgg cgtcccgcgg gtgccgatcg
      961 ccttgagcaa aagcgcgcct acggacagat catgaacgcg agcttcaggc tgtactggcg
     1021 ccacctgtcg gcctttgcgg tgctgggggc gatcgcgatc ccgatcgttg gcggcaccca
     1081 ggccctcggc gggtggctgg gggacatgtc cggcggggac gggttgctgc agacgctctc
     1141 cgacctggtc aacagtttcg gccggccggc ggcccaggcg ctgatctcgg cctttctgat
     1201 cgtgttcatc cgtgacctgg ttcaggggca gaccgcgacc gtgaggacgg cgctcgacgg
     1261 cacccgggaa cggttctggc gcgtcgtgat cgcaaagatg ctcgcgattg ccggagtgac
     1321 cctgcttgct ctgaccgtga tcggaattcc cttcgcggtc cgcttcctcg tcaactggac
     1381 cttcgtccag caggaggtga tcttcaccga cctgtcgatc cgtgactcct tccgggccag
     1441 cagcgatctg gtccgcgggc actggtggcg aacggtccgg accatggtcc cgctgctctt
     1501 gctgctgacc gtgctgggac cgctgcttgg tctctttctg atcttcactc ccttgccgct
     1561 gcttctggtc aacctgatcg gatcggtggt ctttgcgatc gcgattccgt tcacgaccgc
     1621 cggcacgacc ctgctctact tcgatctgcg agcccgccag caggaacacg gggtggtgcc
     1681 gcgccgctcc tgggccgtct ggcgaccggc gactttcggc cggccggtcg ggcagcccac
     1741 cgcctgaagc ctgcgccggg gcatccaacc ggcggtaccg cctcttgaca ttttgagtat
     1801 cacgatatat cgttatgtat cgtgagatat tcgaaacgaa aggtttttca gatgtttgat
     1861 tcagatttcg acccgcgcga ccgcgggcga ttcgagggtc gctgcggcga tcccggcttc
     1921 ggccccgcgg ccggtgacgg ccagccgggt ttcggcccgg agacggattc gggccggcgc
     1981 cgctaccggc acgggccggg aaggggcgga ccccacggtt tcggcggcgg ccaccatggc
     2041 cgggggcctg gtcgccccgg acgcggcagg agggcccggc gaggtgacat ccggcaggcg
     2101 atcctgctgt tgctcgaaga ggagccccgc aatggctacg gcctgatgca ggaggtcgaa
     2161 gagcgaagcg gcggtgcctg gcgtccgagc cccggctcgg tctacccggc gctctcccag
     2221 ctcgaggacg aaggtctggt cgagatggcc gaatccgaag gccgcaagga cttctccctg
     2281 accggcgccg gcagagaatg ggtcgaggag catcgcgagg agatcggttc tccctgggat
     2341 accgaaggcc gcgggactcc ccgggaactc ggttccctga ggaagtcgac ccaggcgctg
     2401 gcgatggccg ccaggcaact ggcccatacc ggcacaccgg cccagctcga ggaagcgcga
     2461 aagctcgtcg atgaagcccg gcgaaagctc tacgggatcc tcgcccagag cgaagaggag
     2521 caggagcagt cctgagcttt cgcgggccgg cgatctagaa gagtgaaagc gtggtgattc
     2581 cgtcgccgat cagtttggcg gcgatcacca cgcagatcac ggccatgatc acgccgttgt
     2641 tgcgggccat gctctccttg agcctggcca gcatcgaatc ggcccggtca cccatcagga
     2701 agtagatccc gaccgggacg gccgccccga tgctggcgat caaggcgtag accagtaccg
     2761 aagtcagctg gtcggcgctg ccggcgtcca cctgcccgat cgcggtagcg gcggccatgg
     2821 tcaggatcag gttcttggga ttgaccgcgc cgagcagaaa ggcgaggccg gtcgcac
//
LOCUS       DAOQMF010000176         2876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_19952_length_2876_cov_4.349876, whole genome shotgun sequence.
ACCESSION   DAOQMF010000176 DAOQMF010000000
VERSION     DAOQMF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19952_length_2876_cov_4.349876"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /gene="glsA"
                     /locus_tag="PLV77_03925"
     CDS             complement(<1..1813)
                     /gene="glsA"
                     /locus_tag="PLV77_03925"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HNC15025.1"
                     /translation="MTESVGNNRTASPVQALLERLHERHAGDLSGEVASYIPELAGAD
                     PEWFGISMVTADGSVYEVGDTRREFTIQSISKPLTFALALEAIGEGEVRRHVDVEPSG
                     EAFNSITLQPGTGLPLNPMVNAGAIATASLIEPLGFEKPIDRILHEYSQFAGRHLKVD
                     EQVFTSENETGNRNRAIAYLLKNAGVIDRDVEEVVETYFRQCSTLVTCRDLATMAATL
                     ASTGVNPVTGERVVREDTVRNVLSVMASCGMYDAAGDWLYTVGLPAKSGVAGGIIAVL
                     PGQLGVAVYSPPLDLHGNSVRGVKVCEDLSDELHLHVMSPARRPPPPVRIERTCIDSR
                     SKRLRGPAEQQILAEHGRRVKVLELQGELSFAAGELVTSSALHRAEHHDFTVLDFRAV
                     RAIDLIDVPIFSHLVESCEEAGSEIAFSDVDRHPVFLEELTRHREREGHHPLRTFPDL
                     DLALEWCEQGLIEKYGDLESDPELSLAQHGTTLGMSVEEIEELSSHLEHVSYEAGEMI
                     YGAELVAPDQMLLVTAGKISLLAEDDEGGHRRLATLSPGMTLGEISMIAGTPLAGMPR
                     ADTEVKAWVLTAGDLEVLRARNPGLVVTLLENLLRQVA"
     gene            1886..>2876
                     /locus_tag="PLV77_03930"
     CDS             1886..>2876
                     /locus_tag="PLV77_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNC15026.1"
                     /translation="MLPLADTTVDLARLQFATTSIYHFLFVPLTLGLTPLVAVMQTLW
                     HRTGDERWYRLTRFFGTLMLINFAIGVATGIVQEFQFGMNWSTYSSYVGDVFGAPLAI
                     EGLAAFFLESVFLGLWIFGWDLLPKRVHLAMIWLAAIGTWLSAYFILVANSWMQRPVG
                     YELNGDKAQLNDVGALLSNRFAIHAFEHTLLVGLSTAALFIFGIACWHLVKGRNVETF
                     RKAAALALIVGVPATGINLAVGSGFGIVTTDYQPMKIAGSEALWNTEQPAGFSLFQIG
                     GFSQDDQDPSVVIQIPKLLSFLATGSVNGKVEGMNQLQADYQQKYGPGDYLPPV"
BASE COUNT          468 a          974 c          901 g          533 t
ORIGIN      
        1 gggcgacctg cctcagcagg ttctccagca gggtcacgac cagccccggg ttcctcgccc
       61 gcaacacctc gaggtcgcct gcggtcagaa cccacgcctt gacctcggtg tcggcgcggg
      121 gcatgccggc cagcggcgtc ccggcgatca tcgagatctc gcccagggtc atccccggtg
      181 agagcgtcgc caggcgccgg tgcccgcctt catcgtcctc ggccagcagg ctgatcttcc
      241 ctgccgtgac cagcagcatc tggtcgggtg cgaccagctc cgctccgtag atcatctccc
      301 ccgcctcgta ggaaacgtgc tcgaggtgtg aactcagctc ctcgatctct tcgacgctca
      361 tgccgagggt cgtaccgtgc tgggcgaggc tgagttccgg gtcggattcg aggtcgccgt
      421 acttctcgat cagcccctgc tcgcaccatt cgagggcgag gtcgaggtcc gggaaggtcc
      481 tgagcgggtg gtggccttcg cgttcccgat gccgggtcag ctcttccaga aagaccggat
      541 ggcggtcgac atccgagaag gcgatttcac tgccggcctc ctcgcagctc tcgaccaggt
      601 gactgaagat cggcacgtcg attaggtcga tcgccctgac cgcccggaag tcgagcaccg
      661 tgaagtcgtg gtgttcggcg cggtggaggg ccgaactggt gaccagctcg ccggccgcga
      721 aggaaagctc gccctgcagt tcgaggactt tcacccggcg gccatgctcg gcgaggatct
      781 gctgctcggc cgggccccgc aaacgctttg accgcgagtc gatgcaggtc cgctcgatcc
      841 tgaccggagg cggtggccgc cgggccgggc tcatcacatg caggtgaagc tcgtcggaga
      901 ggtcttcgca gaccttgacc ccgcgaacgc tgttgccgtg caggtcgagc ggcggcgagt
      961 agacggccac cccgagctgg ccgggcagaa ccgcgatgat cccgccggcc acgccggact
     1021 tcgccggcag gccgaccgtg tagagccagt cgccggccgc gtcgtacatg ccgcaggagg
     1081 ccatcacgct gagcacgttg cggaccgtgt cctcccggac cacccgctcg ccggtcaccg
     1141 ggttgacccc ggtcgaagcg agtgtggccg ccatggtcgc caggtcccgg caggtgacca
     1201 gggtggagca ttggcggaag taggtttcga ccacctcttc cacgtcccgg tcgatcaccc
     1261 cggcgttctt cagcagatag gcgatcgccc ggttgcggtt tccggtctcg ttctcggagg
     1321 tgaagacctg ctcgtcgacc ttcaggtggc ggccggcgaa ctgcgagtac tcatggagga
     1381 tccggtcgat cggcttctcg aatccgagcg gctcgatcag cgaggccgtg gcgattgccc
     1441 cggcgttgac catcgggttc agcggcagcc cggtcccggg ctgcagggtg atcgagttga
     1501 aagcctcccc gctcggctcc acgtcgacat gccggcgaac ttccccttcc ccgatcgcct
     1561 ccagcgccag ggcaaaagtc agcggcttgg agatcgactg gatcgtgaac tcccgccgcg
     1621 tgtcccccac ctcgtaaacc gacccgtcgg cagtaaccat cgaaatcccg aaccactccg
     1681 gatccgcccc cgccagctcg gggatgtagc tcgccacctc ccccgaaaga tcccccgcat
     1741 gccgctcatg caaccgctcc agcaacgcct gaacaggcga ggccgtgcgg ttattgccga
     1801 cggattcagt catggatagg tacctgccat cgtattgacc ggctcaaaca acggttctgt
     1861 ccggtttgtg ccgaagtatg caggtgtgct gccgctggct gacacgaccg ttgacctggc
     1921 gcggcttcag ttcgcgacca cctcgatcta ccatttcctc ttcgttccgc tgaccctcgg
     1981 gctgaccccg ctggtggccg tgatgcagac cctctggcac cggaccgggg acgaacgctg
     2041 gtaccggctg acccgcttct tcgggaccct gatgctgatc aacttcgcga tcggggtggc
     2101 gaccggaatc gtccaggaat tccagttcgg gatgaactgg tcgacctact cgagctatgt
     2161 cggtgacgtg ttcggggcgc cgctggcgat cgagggtctg gccgccttct tcctcgagtc
     2221 cgtcttcctt ggcctgtgga tcttcggctg ggacctgctg ccaaaacggg tccacctggc
     2281 gatgatctgg ctggccgcaa tcggaacctg gctctccgcc tacttcatcc tggtcgccaa
     2341 ttcatggatg cagcggccgg tcggatacga actgaacggc gacaaggccc agctcaatga
     2401 cgtcggtgcc ctgctgagca accgcttcgc gattcacgcc ttcgaacaca ccttgctggt
     2461 cggcctctcg accgcggccc tgttcatctt cggcatcgcc tgctggcatt tggtcaaggg
     2521 caggaatgtc gagaccttcc gcaaggcggc tgccctggcc ctgatcgtcg gagtgccggc
     2581 gaccggaatc aatctcgcgg ttggcagcgg cttcggaatc gtcaccaccg attaccagcc
     2641 gatgaagatc gccggctccg aggctctgtg gaacaccgaa caaccagccg gcttctcgct
     2701 cttccagatc ggcggcttca gccaggacga ccaggatccg tcggtggtga tccagattcc
     2761 gaaactgctc tcgttcctgg ccacgggatc ggtgaacggc aaggtcgaag gcatgaatca
     2821 gctccaggcc gattaccagc agaagtacgg ccccggcgac tacctgccac cggtcc
//
LOCUS       DAOQMF010000177         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20223_length_2854_cov_3.809932, whole genome shotgun sequence.
ACCESSION   DAOQMF010000177 DAOQMF010000000
VERSION     DAOQMF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20223_length_2854_cov_3.809932"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..665)
                     /locus_tag="PLV77_03935"
     CDS             complement(192..665)
                     /locus_tag="PLV77_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC15027.1"
                     /translation="MSVFLTIERAEAPAGHEPSRVPLVDADGQVTIDPWWGTIQPQRL
                     HPGIPTWGELEVIAHIEAGGRLIDTRLPEYASASGTIPGSLVLPWETITDHLEVFDPA
                     SVTVLFCNGPQCAATPRAVGRLLDAGVEPESLAYYRGGIQDWVGLGLPTAPVSGG"
     gene            complement(765..1316)
                     /gene="cysC"
                     /locus_tag="PLV77_03940"
     CDS             complement(765..1316)
                     /gene="cysC"
                     /locus_tag="PLV77_03940"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF002059.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNC15028.1"
                     /translation="MSDSKGFTLWFTGLSGAGKTTISEIVARELTDRGSKLEILDGDI
                     VRENLSKGLGFSKEDRDINIRRIAFVADLLSRNGVPVITAAISPYRALRDEARELMGD
                     RFIEVYIEASVEVCAERDVKGLYAKAFSGEIKEFTGVSDPYEAPENPEIALKTAEEEP
                     EESAARLLAYLEERQLIPAVVAS"
     gene            complement(1320..2387)
                     /gene="sat"
                     /locus_tag="PLV77_03945"
     CDS             complement(1320..2387)
                     /gene="sat"
                     /locus_tag="PLV77_03945"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HNC15029.1"
                     /translation="MTELALQLSERQTADFEMIGNGGFAPLTGFQGSADWQRVCDEMR
                     TADGDYWPIPITLATDLECEVGDVVSLTSDEGKALGRITIEEIFERDVEHEAKSVYQT
                     NDQEHPGVAAIREEGARCLAGPIEVDALPDHEEAFQRRYLTPAESKQAFADRGWKRVV
                     AFQTRNPIHRAHEYLTKAALEVSDGLFIHPLIGKTKAGDIPADVRMKCYELLMEDYYP
                     NDRVILGVNPSKMHYAGPREAVLHAIVRRNYGCTHFIVGRDHAGVGDYYGTYDAQKIF
                     EGIDIDELGIEPLMFEHTFWCNECEGLASNKTCPHDAEAHLFLSGTKVREMLGNGEMP
                     PPEFSRPEVAQILIDAYKEDN"
     gene            complement(2481..>2854)
                     /locus_tag="PLV77_03950"
     CDS             complement(2481..>2854)
                     /locus_tag="PLV77_03950"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNC15030.1"
                     /translation="QLWKRDPDACCGIRKVDPMARALASGVDCWVAGIRREDSATRAG
                     SPKFMWDRKFNIWKLNPIADWSERDVWTYLHKHGIPYNPLHDQGYPSIGCTHCTRPVL
                     PGQSSRDGRWSGSGKTECGIN"
BASE COUNT          481 a          891 c          961 g          521 t
ORIGIN      
        1 cagccgcttc ggcgcctacc agacctacga cctgggccag cccgaagtcc cgcccaggcc
       61 ggcgccctga cctgaacccc cgcgggatcc cttcccgccg gcccttcccc atgtccgggc
      121 agccgcggat ggagccaccg ggggcggggc tccaccgcga ctctggtccg ggctctcccg
      181 cccggaagct ttcagccgcc cgaaacgggg gcggtgggga ggccgagccc gacccagtcc
      241 tggatcccgc cgcggtaata ggcgagagat tcgggctcga ccccggcgtc cagcagcctt
      301 cccaccgctc tgggggttgc cgcacactgc ggcccgttgc agaagagcac cgtcacgctt
      361 gcgggatcga acacctccag gtggtcggtg atcgtctccc agggcagaac cagcgagccg
      421 gggatcgtcc cgctcgccga tgcgtattca ggcagccggg tgtcgatcag acgcccgccc
      481 gcctcgatgt gcgcgatgac ttccagctca ccccaggtgg gaatcccggg gtggagccgc
      541 tgcggctgga tcgtgcccca ccaggggtcg atcgtgacct gtccgtcggc atcgaccagc
      601 gggacccggc tgggctcgtg cccggccggc gcctcggcgc gttcgatggt aaggaaaacg
      661 gacatggcca aagtatatca ctacacaact aaacagttgt ttacagaggg aagaaggggc
      721 gggctcgaaa gcccgccccg gcgaagtact tatttggcgt ttgcctacga ggcgacgacc
      781 gccgggatga gctggcgctc ttccaggtag gcgagcagcc tggcagccga ttcctccggc
      841 tcctcttccg cggtcttgag ggcgatctcc gggttttccg gcgcttcgta cgggtcggag
      901 accccggtga attccttgat ctcgccggag aaggccttgg cgtagagacc cttcacgtcg
      961 cgttcggcgc agacttcaac cgacgcctcg atgtagacct cgatgaagcg gtcacccatc
     1021 agctctcgag cctcgtcgcg gagtgcccgg tagggcgaga tcgcggcagt gatcaccggg
     1081 acgccgttgc gggaaagcag gtcggcgacg aaggcgatcc ggcggatgtt gatgtcacga
     1141 tcctccttcg agaagccgag gcccttcgag aggttttcgc gaacgatgtc accatcgaga
     1201 atctcgagct tggagccgcg gtcggtcagc tccctggcga cgatctcgga aatggtggtc
     1261 ttgccggcac cggagaggcc ggtgaaccag agggtgaatc ccttgctgtc tgacattggt
     1321 tagttgtcct ccttgtaagc gtcaatcaag atctgggcga cctcggggcg cgagaactcg
     1381 ggcggcggca tttcgccgtt gccaagcatc tcgcggacct tggtgccgga aaggaaaagg
     1441 tgcgcttcgg cgtcgtgagg gcaggtcttg tttgaagcca ggccctcgca ttcgttgcac
     1501 cagaaggtgt gctcgaacat cagcggctcg atcccgagct cgtcgatgtc gattccttcg
     1561 aagatcttct gggcgtcata ggtgccgtag taatcaccga cgccggcgtg gtcgcggccg
     1621 acgatgaagt gggtgcagcc atagttgcga cgcacgatcg cgtggagcac ggcctcacgg
     1681 gggccggcgt aatgcatctt cgaggggttg accccgagga tcacccggtc gttcgggtag
     1741 tagtcctcca tcagcagttc gtagcacttc atccgcacgt cagccgggat gtcgccggcc
     1801 ttggtcttgc cgatcagcgg gtgaatgaag agaccgtcgg aaacctcgag cgccgccttg
     1861 gtcaggtact cgtgggcgcg gtgaatcggg ttgcgggtct ggaaagccac gacccgcttc
     1921 cagccgcggt cggcgaaggc ctgcttcgac tcggccgggg tcaggtagcg gcgctggaag
     1981 gcttcctcgt gatcgggcag ggcgtcgacc tcgatcgggc cggcgaggca acgggcgccc
     2041 tcctcgcgga tcgcagcgac cccggggtgc tcctggtcgt tggtctggta gaccgacttc
     2101 gcttcatgct cgacgtcgcg ctcgaagatc tcctcgatcg tgatccggcc gagcgccttg
     2161 ccttcgtccg aggtcaggct gaccacgtcg ccaacctcac attcgaggtc ggtggccagg
     2221 gtgatcggga tcggccagta gtcaccgtcg gcggtccgca tttcgtcgca gacacgctgc
     2281 cagtccgccg agccctggaa gccggtcagg ggtgcgaaac ccccgttccc gatcatttcg
     2341 aagtctgcgg tttgacgctc ggacagctgg agggcaagtt cagtcatggt ttggttcata
     2401 aggaggaggg aatgttgaca ggaaaggtga agaatcggac agcctagccg ttatgggcgg
     2461 caaatgccgt ccgacagcgg ctagttgatg ccgcactccg tcttgcccga gccggaccag
     2521 cgcccgtcac gggaagactg gccgggcaaa acgggacggg tgcagtgggt gcagccgatc
     2581 gagggataac cctgatcgtg cagggggttg taggggattc cgtgcttgtg aaggtaggtc
     2641 cagacgtcgc gttcggacca gtcggcgatc gggttcagct tccagatgtt gaacttcctg
     2701 tcccacatga acttcggcga gccggcccgc gttgccgaat cctcgcgccg gatcccggcg
     2761 acccagcaat cgactcctga tgcgagcgcc cgggccatcg gatcaacctt gcggatgccg
     2821 cagcaggcgt ccgggtcgcg cttccagagc tggt
//
LOCUS       DAOQMF010000178         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20410_length_2839_cov_3.344109, whole genome shotgun sequence.
ACCESSION   DAOQMF010000178 DAOQMF010000000
VERSION     DAOQMF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20410_length_2839_cov_3.344109"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLV77_03955"
     CDS             <1..712
                     /locus_tag="PLV77_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC15031.1"
                     /translation="QQLSIRMAKQLGSRVQLNAPVRQIRQEKGLCYVTSDKGTWRARQ
                     VIVAVPPAVSAFIDYDPILPSDRAQLIQRFPQGNAIKVQAIYDTPFWREEGLSGYVNS
                     DEGPVKLMYDNSPHDGSAGVLLGFIEGQEARLAGRMSPAARKAAVLANFKKTIGNAAG
                     SPKAYVEKNWAEEEWTRGCYGGFLPPGVLTGFGPALRKPHGRIHWAGTEHAEIWNGYM
                     DGALRSGEDAARAALARL"
     gene            complement(761..2473)
                     /locus_tag="PLV77_03960"
     CDS             complement(761..2473)
                     /locus_tag="PLV77_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC15032.1"
                     /translation="MSEEIWERKVPPMPEDPDVVVIGSGLAGLTAAAYLAANDQRVLV
                     LEQYDVAGGCSQTFRRKNKWQFDVGLHYIGGVKTGEIARILQGIGLHERIEFPELDPD
                     GFDTFVFPDLEFKVPKGWDNYVGRLVETFPEEEEGLRGVTDVMRNLMDELREVGIPDS
                     NTDLAEYVEKAPTVVSWGMRSVNELFEEHYLSERSRAVLIGQAGDYATPPSRTPVILH
                     AGLVDHYLQEGAFYVRGGGQELAGHLVDLIHSYGGRVRTHAEVEHIATASTEDGGHRV
                     TGVELKGGEVIDTGNVIATGDIKHLFLDLLDSEAVPEGIREQVKAYRMATPLFTVYLG
                     LDFDITERMPATNYWLHSRYDQEAYYDPIAAGEFDTDPPVYISSASAKDPGHEGHAPE
                     GCSTVELMTWATPDPEAWGVDESEDAPKYSRQAAYREAKDRLTEQLIDRAEDQLGEIR
                     SHILWKEAATPMTQTRYTLSTGGSSYGIELATDQFGPLRLEYRTPIEGLVLGGVSSRR
                     GHGIVGAMSGGVESASAVLGRNLRAEVAGGEVFGEPAILTSGGEGWDALEACRKLQVK
                     ALRA"
     gene            2557..>2839
                     /locus_tag="PLV77_03965"
     CDS             2557..>2839
                     /locus_tag="PLV77_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC15033.1"
                     /translation="MALKHAVLAALSTEPGSGYELSKRFDASVANFWPASRQQIYREL
                     DRLEEDGLATARLVRQERRPDKRIFRLTAGGHEAVVEFIRAETGPTVIRD"
BASE COUNT          473 a          940 c          935 g          491 t
ORIGIN      
        1 gcagcagctt tcgatccgga tggcgaaaca gctcggctcc cgggttcagc tcaacgcgcc
       61 ggtccggcag atccgccagg agaagggcct ttgttacgtg accagcgaca agggcacctg
      121 gcgggccagg caggtgatcg tggcagtgcc gccggcggtc agtgccttca tcgattacga
      181 cccgatcctc ccttccgacc gggcccagct gatccagcgc ttcccacagg gcaacgcgat
      241 caaggtgcag gcgatctacg acaccccgtt ctggcgggag gaagggctct ccggatacgt
      301 caactcggat gagggcccgg tcaagctgat gtacgacaac tccccccacg acggctcggc
      361 cggcgtgttg ctcgggttca tcgagggcca ggaagcgagg ctggccggac ggatgagccc
      421 ggccgcgcgc aaggccgccg tgctcgccaa tttcaagaag acgatcggca acgcggccgg
      481 ttcacccaag gcctacgtcg agaagaactg ggccgaggaa gagtggaccc gcggctgcta
      541 cggcggcttt ttgcccccgg gtgtcctgac cggtttcgga ccggcgctgc gcaagcctca
      601 cggtcgcatc cactgggccg ggaccgagca cgccgagatc tggaacggct acatggacgg
      661 cgccctccgt tcaggcgaag acgctgccag ggcggccctg gccagactct gaaacgcacc
      721 tccggcgagg agcgccgggg aaggagcgag gagcgcccgg tcaggcgcgg agggccttga
      781 cctgaagctt gcggcaggct tcgagggcgt cccagccttc gcctcccgaa gtgaggatgg
      841 ccggctcgcc gaagacctcg ccgccggcga cctcggcccg gaggttccgg ccgaggacgg
      901 ccgaggcgga ttccaccccg ccgctcatcg ccccgacgat gccgtgaccg cggcggctcg
      961 agactccgcc aaggaccagg ccctcgatcg gtgttctgta ctcgaggcgg agcggaccga
     1021 actggtcggt ggccagttcg atcccgtagg aggaaccacc ggtggaaagc gtgtacctgg
     1081 tctgggtcat cggcgtcgcc gcttccttcc agaggatgtg cgaacggatc tcgccgagct
     1141 gatcctcggc ccggtcgatc agctgctcgg tcaggcggtc tttcgcctcg cggtaggcgg
     1201 cttgccggct gtacttcggg gcatcttcgc tctcgtcgac tccccaggct tcggggtcgg
     1261 gggtggccca ggtcatcagc tcgacggttg aacatccttc gggcgcatga ccttcatgac
     1321 ccggatcctt ggcgctggcc gaggagatgt aaaccggggg gtcggtgtca aactcgccgg
     1381 ccgcgatcgg gtcgtagtaa gcctcctggt catagcggct gtgcagccag taattggtgg
     1441 ctggcatcct ctccgtgatg tcgaagtcga gccccagata gaccgtgaag aggggggtcg
     1501 ccatgcgata ggccttgacc tgttcgcgga tcccttccgg cacggcctcg gagtcgagca
     1561 ggtcgaggaa gaggtgcttg atgtctccgg tcgcgatcac gttcccggta tcgatcacct
     1621 cgccgccttt cagctcgacc ccggtcacgc ggtggccgcc gtcttcggtc gaagcggtgg
     1681 cgatgtgctc gacttcggcg tgagtgcgaa cccttccccc gtagctgtga atgagatcga
     1741 cgaggtgacc ggccagctcc tgcccgccgc cccggacgta gaaggcaccc tcctgcaggt
     1801 aatggtcgac cagcccggcg tggaggatga ccggagtgcg tgagggagga gtcgcgtagt
     1861 caccggcctg gccgatcaac acggcgcgag atcgctcgct gaggtaatgc tcctcgaaga
     1921 gttcattcac cgagcgcatg ccccagctga ccacggtcgg ggccttctcg acgtattccg
     1981 ccaggtcggt gttgctgtcg gggatgccga cctcacgcag ttcgtccatc aggttccgca
     2041 taacgtcggt gactccccgc agaccctcct cttcctcggg aaaggtctcg accagccggc
     2101 cgacgtagtt gtcccagccc ttcggcacct tgaactcgag gtccgggaag acgaaggtgt
     2161 cgaacccgtc ggggtcaagt tccgggaatt cgatccgttc atgcagcccg atgccctgca
     2221 ggatgcgggc gatctcaccg gtcttcaccc cgccgatgta atggaggccg acgtcaaact
     2281 gccacttgtt cttgcgacgg aaggtctggc tgcagccccc ggccacgtcg tactgctcga
     2341 gcacgagcac ccgttggtcg ttggccgcga gataggccgc agcggtcagg ccggccaggc
     2401 ccgaaccgat gaccacgaca tcgggatcct ccggcatcgg cggcaccttg cgctcccaga
     2461 tctcttcact cattccgtcg tcgacctttc tatactcaaa gatgtgacta ctcaatttgt
     2521 tgagtatagg gcgatacccg atgggagtaa agcccggtgg ctctgaagca tgcggtcctc
     2581 gccgcactca gcaccgagcc cggttccgga tacgagctct cgaagcgctt cgacgcctcg
     2641 gtcgcgaact tctggccggc ctcccggcag cagatctatc gcgagctgga ccgcctcgag
     2701 gaggacggcc tcgccaccgc ccgcctggtc cgccaggagc gccggcccga caagcggatc
     2761 ttccgcctga ccgcgggcgg ccacgaggcc gtcgtcgagt tcatccgcgc cgagaccggc
     2821 ccgacggtga tccgcgacg
//
LOCUS       DAOQMF010000179         2838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20423_length_2838_cov_3.288897, whole genome shotgun sequence.
ACCESSION   DAOQMF010000179 DAOQMF010000000
VERSION     DAOQMF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20423_length_2838_cov_3.288897"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /gene="ligA"
                     /locus_tag="PLV77_03970"
     CDS             <1..1221
                     /gene="ligA"
                     /locus_tag="PLV77_03970"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNC15034.1"
                     /translation="LDRREDLDFEIDGVVVKIDDIGLWNELGNAGREPRWAIAWKFPP
                     TTATTKLLEIEWNVGRTGHLVPWAKLDPVRVGGVTVSNATLHNEEDLERKDVRAGDEV
                     VVLRAGDVIPQVISALPQKRPKGARKPVPPKECPACGTEVIKPEDSVFSICPNRSGCP
                     GQIFQHIKHFVSRGAMDIEGLGEKQVARFLEEGLISDVADIYALRQEQLEALDRMGET
                     SSGNLIAEIEKSREIPFPRVLYALGLIGVGSVTAEALADEFGSIDALHEADPERIEQT
                     EGVGPVIARQIAESLADEGTWALVERLKAAGLKMELAEDERRPEGGPLEGKTVVLTGT
                     LPDLTRDEAGAMVKRAGGKVTGSVSKKTDFVVAGESAGSKLEKAEKLGVTVLDREGLD
                     QLIAGNLPPEPEGD"
     gene            complement(1223..1792)
                     /locus_tag="PLV77_03975"
     CDS             complement(1223..1792)
                     /locus_tag="PLV77_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15035.1"
                     /translation="MGERRRLILAFVLGLVIATAGTATAAKLITGKQIKDGTITSKDL
                     SRALRKQISRSGKAGPTGPAGVTGPAGVTGPTGAVDASGFFTKAESDARYPQIDVVQS
                     SGEYAVSVPSGTMQAIATLLCPDNSLAAKVGYEATGFDQGLVSAQLDASTVDIVINNT
                     GPNPANGTLDVKLNCFQIDPAIVPDPTFP"
     gene            1824..2750
                     /locus_tag="PLV77_03980"
     CDS             1824..2750
                     /locus_tag="PLV77_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15036.1"
                     /translation="MSFSGSARRFLAVAGVAVFTIAVGSASATVPGKNGLLLVSGYTD
                     GGINGSTSRIFTETLTGKSKELLGALDQSYSDPAVSPNGKQIVYSVYPGYRMWLGSFA
                     KPKQAKAITPEESDVLNLETVFAPDGKSIYFSKKYYLDSGVFWHLKKYTFKTKKTTSY
                     KVDTKLDWGFSDVSPNGRYLAYNRGGDEDASKIRLLDTRTGKSHTVNTKGAALGPAFS
                     PDGKSMVYTAQYGDAWELVQSRLDGKNARRLTRTGAINFDPHYSPDGKMIAFTQGVEG
                     NKKIGILTLKTGKIRYLVATGDYAEVEQWLRK"
BASE COUNT          539 a          882 c          953 g          464 t
ORIGIN      
        1 ctggatcgcc gcgaggacct cgacttcgag atcgacggcg tggtcgtcaa gatcgatgac
       61 atcggcctct ggaacgagct cggcaacgcc ggccgcgagc cgcgctgggc gatcgcctgg
      121 aagttcccgc cgaccacggc gaccacgaaa ctgctggaaa tcgagtggaa cgtggggcgg
      181 accggccacc tggtgccctg ggcgaagctc gacccggtcc gggtcggcgg ggtcaccgtc
      241 tccaacgcca cgctccacaa cgaggaggat ctcgaacgca aggacgtgcg ggccggcgac
      301 gaggtggtgg ttctgcgggc cggggacgtg atcccccagg tgatttccgc cctgccacag
      361 aagcggccga agggggcgag gaagccggtc ccgccgaagg agtgcccggc ctgcgggacg
      421 gaagtgatca agccggagga ttcggtcttc tcgatctgcc cgaaccgttc cggctgcccc
      481 ggccagatct tccagcacat caagcacttc gtctcccgcg gagcgatgga catcgagggc
      541 ctcggtgaaa agcaggtcgc ccgtttcctc gaggaaggcc tgatcagcga cgtcgctgac
      601 atctacgccc tgaggcagga gcagctcgag gcgctggacc ggatggggga gacctcctcc
      661 ggcaacctga tcgccgagat cgagaagtcc cgggagatcc ccttcccccg ggtcctctac
      721 gcgctcggtc tgatcggcgt tggatcggtt acggccgaag ccctggccga cgagttcggt
      781 tcgatcgacg cgttgcatga ggccgacccg gagcggatcg agcagaccga gggggtcggt
      841 ccggtcatcg ctaggcagat cgccgagtca ctggccgacg aagggacctg ggctctggtc
      901 gagcgcctca aggcggccgg cctgaagatg gaactggccg aggatgagcg tcgtcccgag
      961 ggcggcccgc tggaaggaaa gaccgtcgtc ctgaccggca ccctgcccga cctgacccgt
     1021 gatgaagccg gggcgatggt caagcgggcc ggtggcaagg tcaccggttc ggtctcgaag
     1081 aagaccgact tcgtggtggc cggagagtca gccgggtcca aactcgagaa ggccgagaag
     1141 ctaggggtga ccgttctcga ccgcgagggt cttgaccagc taatcgccgg gaacctcccc
     1201 ccggagcccg aaggggactg attcagggaa aggtcggatc cggcactatc gccgggtcga
     1261 tctggaagca gttgagcttg acgtcgagag ttccgtttgc cgggttgggg ccggtgttgt
     1321 ttatgacgat gtcgacggtg ctggcgtcga gctgggcgga aaccaggccc tgatcgaagc
     1381 cggtggcctc atagcccacc ttcgccgcga gggaattgtc cgggcagagc agtgtcgcga
     1441 tggcctgcat cgtcccggaa ggaaccgaaa ccgcgtactc cccgctcgac tggaccacat
     1501 cgatctgcgg gtagcgggcg tcgctttccg ctttcgtgaa gaaaccggag gcgtcgaccg
     1561 ccccggtggg cccggttaca cccgccggtc cggtcacgcc ggccggaccg gtcgggccgg
     1621 ccttgccgga tctgctgatc tgcttgcgaa gcgccctgga cagatccttc gaggtgatcg
     1681 tgccgtcctt gatctgcttt ccggtgatca gctttgccgc agtcgccgtc ccggcggtgg
     1741 cgataaccag gccgaggaca aaagcgagta tcagccttct ccgttctccc atgtgtcatt
     1801 cataaccgat taggctgggc ggcatgagtt tttcagggag tgccagaagg tttctggccg
     1861 tggcgggggt cgcggtcttc acgatcgcgg ttggcagcgc ctcggcgacg gtgccgggca
     1921 agaacgggct gctgctcgtt tccggctaca ccgatggtgg catcaacggg agcacgagcc
     1981 gcatcttcac cgagaccctg accggaaagt cgaaggaact gcttggcgcc ctcgaccagt
     2041 cgtattccga tccggcggtc tcaccgaacg gcaagcagat cgtctactcg gtctatcccg
     2101 gctaccggat gtggctcggc tccttcgcca agccgaagca ggcgaaggcg atcaccccag
     2161 aggaaagtga cgtgctgaac ctcgaaaccg tattcgcgcc ggacggcaag tcgatctact
     2221 tctcgaagaa gtactacctc gattcggggg tcttctggca cctgaagaag tacaccttca
     2281 agacgaagaa gacgacgtcc tacaaagtcg acaccaaact ggactggggc ttctccgacg
     2341 tttcgcccaa tggccgttac ctcgcctaca accggggcgg cgacgaggac gcctcgaaga
     2401 tcaggctgct ggacacgcgc accggcaaga gccacacggt caacacgaaa ggcgcggcgc
     2461 tggggccggc gttctccccc gatggcaagt cgatggtcta caccgcgcag tacggggacg
     2521 cctgggagct ggtccagtca cgccttgacg gcaagaacgc caggcgactg acccggaccg
     2581 gggcgatcaa cttcgatcct cactattcgc ccgacggcaa gatgatcgcc ttcacccagg
     2641 gggtcgaagg aaacaagaag atcggcatcc tcaccctcaa aaccggcaag atccggtatc
     2701 tcgtggcgac cggcgactac gccgaagtcg agcaatggct caggaaataa ggagtagggc
     2761 gggatccaac ccgccaaccc gccactaaag ccgggcgatc agggcggcga tgcggtcctg
     2821 ggcccggtgg gcggtgtc
//
LOCUS       DAOQMF010000180         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20530_length_2832_cov_2.751891, whole genome shotgun sequence.
ACCESSION   DAOQMF010000180 DAOQMF010000000
VERSION     DAOQMF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20530_length_2832_cov_2.751891"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLV77_03985"
     CDS             <1..373
                     /locus_tag="PLV77_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC15037.1"
                     /translation="LVTGDPAALSAAIENLIVNAIEHGGPRIVVRALVIGKRLRLEVT
                     DNGKAHRAAGRSGSPAEVIARLRGKDRHGHGLPVVREAAREHAGRFEINLDESGSTAT
                     LTLPCATRAPAPAPGLVGTEA"
     gene            407..1021
                     /locus_tag="PLV77_03990"
     CDS             407..1021
                     /locus_tag="PLV77_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15038.1"
                     /translation="MLLAAAGAGILAVSLVGGYSSSVAESYGELRPVVVLTRGLAPGQ
                     VISPKVAAASFEIRQVPARFVPAEALGDPTRAVGLEPVGPMPAGSYLTGPGLMVPGSD
                     RPKRRPLGHGRHAVEISVAGAGALSGGGKVDVLVTTDNDRGGGRTLVAARRVPLISIG
                     RTGQSEAGPGLTEVTLGLTRGEAIRLVDAQTFARRITVLPPAGP"
     gene            1023..2096
                     /locus_tag="PLV77_03995"
     CDS             1023..2096
                     /locus_tag="PLV77_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNC15039.1"
                     /translation="MDDRGRLAAEIRAGLIERRRAGAGTDQEGIEQAIRHAVEARAVM
                     LSEAAQEDLVERILREASGLGPLEVLLDDGSVEEVMVNGPDLVYVERGGKIEPAGVRF
                     EDEEQLRNTIERILAPLGRRVDELSPMADARLPDGSRVNVVIPPLAVDGPAVSIRRFP
                     GRRPTVDDLVQWGTLSAGQAERLGREVAARRSILISGGTGSGKTTLLNALSGFIDAAE
                     RIVTIEDAAELSLAQPHVVRLESRPPSIEGRGEVTIRDLLRNALRMRPDRILIGEVRG
                     AEALDLLTALNTGHDGALSTVHASSATDALARIEVLALMADVGLPLNVIGAQLRRGID
                     LVVHISRTAEGGRKVTEIEEVGQ"
     gene            2093..>2832
                     /locus_tag="PLV77_04000"
     CDS             2093..>2832
                     /locus_tag="PLV77_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC15040.1"
                     /translation="MTGALPAFLAGIAAVLAVVAVGEALGARTPADEGQGPGRGDRTA
                     GETGGGWAGLRAWLAATVEPLRRARREGYLPTSDERFRLALLFSFAAITGGWWLAGPV
                     VGFGLAVAAPMLAGWLISRGRRRYRRSVEKSLPEVSRAIADCLSAGHSPRGALVAATA
                     SLEGPVRSEFQTMRYELEAGAPTTDAIRNLATRFDSPRVDAFATALISQRLAGGDLAA
                     LLRRFAEGAAERDRVAEDARSATAQARFT"
BASE COUNT          405 a          935 c         1076 g          416 t
ORIGIN      
        1 attggtgacc ggggatccgg ccgcgctctc ggcggcgatc gagaacctga tcgtcaacgc
       61 gatcgagcac ggtgggccgc ggatcgtggt gcgtgccctg gtgatcggca agcggctgcg
      121 gctggaggtc accgacaacg gcaaggccca ccgtgccgcg ggacgctccg gcagcccggc
      181 cgaagtgatc gcgaggctgc gtggcaagga ccggcacggc cacgggttgc cggtggtccg
      241 ggaggccgcc cgtgaacatg ccggccgctt cgagatcaac ctggatgagt ccggttcaac
      301 cgcgaccctg accctgccct gcgcgacccg ggccccggct ccggcaccgg ggctggtcgg
      361 gaccgaggcc tgaaccggga tgggccggcg ccggcgggca acgctgatgc ttctggccgc
      421 cgccggtgcc ggaatcctcg cggtgagcct ggtcggcggg tactcctcct cggtcgccga
      481 gagctacggc gagctgaggc cggtcgtggt cctgacccgc ggtctcgctc ccgggcaggt
      541 gatctcaccg aaagtggcgg cggctagttt cgagatccgg caggtgccgg cacgtttcgt
      601 accggcggaa gcgctgggtg atccgacccg ggctgttggc ctggaaccgg tcgggccgat
      661 gccggccggc agctacctga ccgggcccgg gctgatggtg ccgggcagcg accggccgaa
      721 acgcaggccg ctcggtcacg gccggcatgc ggtcgagata tcggtggcag gagccggggc
      781 cctgagcggg ggcggaaagg ttgacgttct ggtcacgacc gataacgacc ggggtggagg
      841 ccggaccctg gtcgcggcgc gacgggtccc tttgatctcg atcggccgga ccggtcagag
      901 cgaggctggc ccgggactga ccgaggtgac cctggggctg acccggggcg aagcgattcg
      961 cctggttgac gcgcagacct tcgcccggcg aattaccgtg ctgccaccgg cggggccctg
     1021 agatggatga ccgggggagg ctggcggccg agatccgggc cggtctgatt gagcggcgcc
     1081 gggccggtgc cggcacagat caggaaggaa tcgagcaggc gatccggcat gcggtcgaag
     1141 cccgggcggt gatgctctcc gaagcggcgc aggaggatct ggtcgagcgg atcctccgtg
     1201 aggcatccgg cctggggccg ttggaggttc tccttgatga cggcagcgtc gaggaagtca
     1261 tggtcaacgg gccggacctc gtctacgttg aacggggcgg aaagatcgaa ccggccgggg
     1321 tcaggttcga agacgaggaa cagctcagga acacgatcga gcggatcctc gccccgctcg
     1381 gcaggcgggt cgacgagctc agcccgatgg ccgatgcacg gctgccggac ggttcacggg
     1441 tgaacgtcgt gatcccgccg ctggcggtcg acgggccggc ggtttcgatc cgccgctttc
     1501 ccgggcggcg gcccacggtt gacgacctgg ttcagtgggg aaccctgagc gccgggcagg
     1561 cggagcggct gggtcgcgag gtggcggcgc gtcgcagcat cctgatcagc ggcggcaccg
     1621 gttccggcaa gaccacgctg ctgaatgccc tgtcgggatt catcgatgcc gccgaacgga
     1681 tcgtgacgat cgaggacgcg gccgagctca gcctcgcgca accccatgtg gtccggctcg
     1741 aatcccgtcc gccaagcatc gagggccgcg gcgaagtgac gattcgcgac ctgctccgaa
     1801 acgcgctgag aatgcgaccc gaccggatcc tgatcggaga ggtgcgcggc gccgaggccc
     1861 tggacctgct gaccgccttg aacaccggtc acgacggagc gctttcaacc gttcacgcca
     1921 gttcggccac cgatgccctg gcccggatcg aggtcctcgc cctgatggcc gatgtcggat
     1981 tgccgctgaa cgtgatcggc gcccagctcc gccgcgggat cgacctcgtc gtccacattt
     2041 cccgcaccgc cgagggcggt cgcaaggtca cggaaatcga ggaggtcggc cagtgaccgg
     2101 cgccctgccc gccttcctgg ccggaattgc cgcggtgctt gccgtggttg ccgtcggcga
     2161 ggcactgggc gcccggaccc cggccgacga ggggcagggt ccgggaagag gcgaccggac
     2221 cgcgggagaa acgggcggcg gctgggccgg gctcagggct tggctggcgg ccacggtcga
     2281 accgcttcga cgggcccggc gtgagggtta cctgccgact tccgacgaac gcttccggct
     2341 ggccctgctg ttcagcttcg cggcgatcac cggcggttgg tggctggccg gcccggtggt
     2401 cggcttcggc ctggcggttg cggcgccgat gctggccggc tggctgatct cccgggggcg
     2461 ccggcgctac cggcggtcgg ttgagaaatc cctgccggag gtctcccggg cgatcgcgga
     2521 ttgcctcagc gccggtcatt caccgcgcgg cgccctggtc gccgcgaccg cctcgctgga
     2581 gggtccggtt cggagcgagt tccagacgat gaggtacgaa ctcgaggccg gggcgccaac
     2641 cacggacgcg atccgcaatc tcgccacccg gtttgactcg ccgcgggtag atgccttcgc
     2701 caccgccctg atcagccagc gactggccgg cggtgacctg gcggcgctcc ttcgccgctt
     2761 cgccgaggga gccgcggagc gcgaccgggt ggcggaggat gcccgatcgg ctaccgccca
     2821 ggcccgcttc ac
//
LOCUS       DAOQMF010000181         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20568_length_2829_cov_5.011175, whole genome shotgun sequence.
ACCESSION   DAOQMF010000181 DAOQMF010000000
VERSION     DAOQMF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20568_length_2829_cov_5.011175"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..2391)
                     /locus_tag="PLV77_04005"
     CDS             complement(388..2391)
                     /locus_tag="PLV77_04005"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNC15041.1"
                     /translation="MGEIGGALNRSVSRSMALALFGIAALFAGDDEAPASTQNLNSTC
                     GNTKVSTACSDTVYGALHNNGEAKVGPRRLVVNQSGKIFSRGTRLFVAHTPLDIGSET
                     VRIKKTDGKAKVSVAICSTAAADPFTQRVESFFTIPSGKGSIGTTFSRTVSGIKNRRL
                     SVRFAGKSLTDNFKYTMTVDRPGDDEIWMPSQTQLNSVYRPVEGFADLHAHHAGGLAF
                     ASGWYIGGMEQGSIYSGDHQKKLKVNLVGVPLTTLRPAHQGKVIPSSDDNSHQQMNLN
                     ALKAAHDNGLGLMVSPAVNSEWLCSVLAYALEINKRQSCQDMESAKYQILKMKQFDEK
                     YGWYDIVTNPWEARSAAYAGRLPVVLGVEVSDLFPKSDGDWRRQLAELYDMGVRVVYV
                     AHESNSRFAGAAYHHTEDLLLPNELKAWFSKEIKFASMKNGRNAIGLSDLGEDLIREL
                     MRRRMLIDVDHASWKAVDEIFDITSANRYYPIYAGHTRPYELLTDYMKYLVPELNTPP
                     RVLDYIKRSGGMVALRTGPEGMETYSESGVANNCSGSVRSLIQMYRYFVDEDVPVAFG
                     SDFNGFVEEIGSRFGRTGCPASTGEFRELQLTSQTRPSSAGAPPSWQRYLDRGMSDIG
                     TEPAVVFDMQKLGVDTAPLENSAQSFLQMWGRAYRSNRTEVQP"
     gene            complement(2434..>2829)
                     /locus_tag="PLV77_04010"
     CDS             complement(2434..>2829)
                     /locus_tag="PLV77_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15042.1"
                     /translation="FTDKIWVPNAGLSPGRERLLGCLDTWYAYSAGSPFRGGCLMTSI
                     STEFDGRPGPVREAIAKSWGGWTRVLRADLDLAIENGELSPPLAPADTITAIESLATG
                     VNQQTQLFGNRHPEKTASAAARVLLGILS"
BASE COUNT          467 a          877 c          942 g          543 t
ORIGIN      
        1 ttcccggctt attcctcgcc agccacccgc aggagcgccc tgtgcacgac ccgctgacgg
       61 ctggaggccc acgacccact cacggctgga ggtccctggc atccacccgc cgaagtttgg
      121 cccgctgacg gctggaggcc cacgacccgc tcacggttgg tggtgaaccc gttttcgcga
      181 gatgcggttt gtcagtaaat agcgctgaca gatcggatct cgatcgattt cccgacccgg
      241 atccggggct ggtgggtatg gatttcgttc atatagacga aatccatacc cacctcccga
      301 aacctgggcc tggacctggc cctggagacg gtgcgggacg ctgggacgcc aacaacttcg
      361 ccctcaatgc cgccggccgg tttcagctca gggctggact tcggtgcggt tgctccggta
      421 ggcgcggccc cacatctgga ggaaggactg ggccgagttc tcgaggggtg cggtgtcgac
      481 cccgagtttc tgcatgtcga agacgaccgc cggttcggtg ccgatgtccg acattccccg
      541 gtcgaggtag cgctgccagc tgggcggcgc accggcggag gaggggcggg tctgggaggt
      601 caactgcagt tcccggaact cgccggtcga ggccgggcag ccggtccgcc cgaacctgga
      661 tccgatctct tccacgaacc cgttgaagtc actgccaaag gcgaccggca cgtcttcgtc
      721 gacgaagtac cggtacatct ggatcagcga ccggaccgag cccgagcagt tgttggctac
      781 gccgctttcg ctgtaggtct ccatcccttc gggtccggtc cgcagggcga ccatgccgcc
      841 actccgcttg atgtagtcaa ggacccgggg cggggtgttg agctccggca cgaggtactt
      901 catgtagtcg gtaagcagct cgtagggtcg cgtgtggccc gcgtagatcg ggtagtagcg
      961 gttggcgctg gtgatgtcga agatttcgtc taccgccttc caggaggcgt gatcgacgtc
     1021 gatcagcatg cgcctcctca tcagctcgcg gatcaggtcc tcgccgaggt cgctgagccc
     1081 gatcgcgttg cggccgttct tcatcgaagc gaacttgatc tccttcgaga accaggcctt
     1141 cagttcgttg ggaagcagca ggtcctcggt gtggtggtag gcggcgccgg cgaaacggct
     1201 gttcgactcg tgggccacgt agacgacccg gacgcccatg tcatagagct cggcaagctg
     1261 acgccgccag tccccgtcgg acttggggaa gaggtcgctc acctcgactc ccagcaccac
     1321 cggcaggcgg cccgcgtagg ccgccgatcg cgcctcccag ggattggtga cgatgtcata
     1381 ccagccgtat ttctcatcga actgcttcat cttgaggatc tggtacttcg ccgactccat
     1441 gtcctggcag gattgccgct tgttgatctc gagcgcgtag gcgagcaccg aacagagcca
     1501 ctcgctgttg acggcggggg agaccatcaa tccgagcccg ttgtcatggg ccgccttcag
     1561 cgcgttgagg ttcatttgct gatggctgtt gtcatccgag ctcggaatca ccttcccctg
     1621 atgggccggc cgcagcgtgg taagcggaac cccgaccaga ttcaccttca gcttcttctg
     1681 gtgatccccg gaataaatcg acccctgctc catgccgccg atgtaccagc cactggcgaa
     1741 ggcgagtccg ccggcatgat gcgcgtgaag gtcggcgaag ccttcgaccg ggcgatagac
     1801 cgagttgagc tgcgtctggc tgggcatcca gatctcgtca tcgcccgggc ggtccacggt
     1861 catcgtgtac ttgaagttgt cggtgaggct cttgccggcg aaccgcaccg agagccgccg
     1921 gttcttgatg ccgctgacgg tccgggagaa agtggtgccg atgcttccct tgccgctcgg
     1981 gatggtgaag aaactctcga cccgctgcgt gaaggggtcg gcggctgcgg tcgaacagat
     2041 tgcgacgctg accttggcct tgccgtcggt cttcttgatc cgcacggtct cggagccgat
     2101 gtccagcggg gtgtgggcga cgaagagccg ggtgcccctg gagaagatct ttcccgactg
     2161 gttgacgacc aggcgacggg gcccgacctt cgcctcgccg ttgttgtgca gcgcgccgta
     2221 aaccgtgtcc gaacacgcgg tcgagacctt cgtgttgccg caggtcgaat tgagattctg
     2281 ggtacttgcc ggggcttcgt catctcccgc gaaaagtgcc gcgatcccaa aaagtgccag
     2341 cgccatcgag cggctgaccg atcggttgag tgctccccca atttccccca tgagacgaga
     2401 ctaaccgcgg acggtcgggc tgcggggaaa ttctcaggaa aggattccga gcaggacgcg
     2461 ggcggcggcg ctggcggtct tttccgggtg gcggttgccg aagagctggg tttgctggtt
     2521 gaccccggtc gccagcgact cgatcgcggt gatcgtgtca gcgggagcga gcgggggaga
     2581 gagctcgccg ttttcgatcg ccaggtcgag gtcggcccgg aggacccggg tccagccgcc
     2641 ccaggatttc gcgatcgcct cccgtaccgg gccgggccgg ccgtcgaatt cggtcgagat
     2701 cgaggtcatg aggcagccac cgcggaacgg gctgccggcc gagtacgcgt accaggtgtc
     2761 gaggcagcca agcaggcgtt cccggccggg agaaagcccg gcgttcggaa cccagatttt
     2821 gtcggtgaa
//
LOCUS       DAOQMF010000182         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20573_length_2829_cov_4.016943, whole genome shotgun sequence.
ACCESSION   DAOQMF010000182 DAOQMF010000000
VERSION     DAOQMF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20573_length_2829_cov_4.016943"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /gene="aspS"
                     /locus_tag="PLV77_04015"
     CDS             <1..1139
                     /gene="aspS"
                     /locus_tag="PLV77_04015"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNC15043.1"
                     /translation="ERCFQIARCFRDEDLRADRQPEFTQVDIEMSFVTGEDVIEVNER
                     LIRHVLAEMGVEVEIPFERMCYDEAIGRFGSDKPDLRYGYELTDLTEILRGTEFKAFG
                     GVIEGGGQVKGINFGQRELSRAQLDGLIDDAKELGAKGLVWAFREGDGWRAPIAKFLS
                     EGELKNLNEAMGAEEGDLMLVAADQPAMVAAILGGLRTRLAERWDLIPADAGNRFCWI
                     VDWPLFEFNEEEDRWDALHHPFTAPKGDFDPENPGEARAEAYDLVWNGVEIGGGSIRI
                     NQPELQSQIFRALGISDEEAEERFGFLLEALRYGAPPHGGIAYGVDRFAALLAGVESI
                     RDVIAFPKTASGGDPLTGAPAPIDDVQMRDVGIELRKKKLPGQG"
     gene            1186..1443
                     /locus_tag="PLV77_04020"
     CDS             1186..1443
                     /locus_tag="PLV77_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15044.1"
                     /translation="MKSIGDGSGRIQINDPRFDDWETVHQFEDLATGLAWRDVLREHG
                     IPAELTSDWELDRYGRGDIHLQVPPGMWSDAEELVSGLDLE"
     gene            1456..1608
                     /gene="ssrS"
                     /locus_tag="PLV77_04025"
     ncRNA           1456..1608
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLV77_04025"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1682..>2829
                     /locus_tag="PLV77_04030"
     CDS             1682..>2829
                     /locus_tag="PLV77_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNC15045.1"
                     /translation="MTSVLRKAGLTGLLAALALAIALPAAASADTQRFYDNWAVGSGP
                     IGIANDRFGDVYVSSNTPAPFLSRYTAEGEFVASFATGGAILGARDVTVDSAGNVYQV
                     NATNNMVRKFSPDGTLITGWGTSGSGDGQFLDPNALATGPDNRIYVGDFGNDRVQWFT
                     PAGVYLGQFATGPMAGLDVGADGNVFVADSLSDEIRVYDPSGTPLDTVGENGSGAGDI
                     DNVSDVDIGDDGRIYAADTSSGMIRVYAPDGSYQGSLGSPGAGDGELNLPNALTADRA
                     GNVWTTQYGGSPRVSLFAYAPRVIGGTNLDFGYSFIGNPPGVQTVLMQNDNYVLPMYV
                     GTASLTSGVDFGLPAPWFECSGVILLPGQVCSVGVDFHPATPGNKTDTL"
BASE COUNT          531 a          875 c          928 g          495 t
ORIGIN      
        1 tcgagcggtg cttccagatc gcccgctgct tccgggacga ggacctgcgg gccgaccgcc
       61 agccggagtt cacccaggtc gacatcgaga tgagtttcgt caccggcgaa gacgtgatcg
      121 aggtcaacga gcgcctgatc cggcatgtgc tggccgagat gggggtcgaa gtggagatcc
      181 ccttcgagcg catgtgttac gacgaggcaa tcggccgctt cggctccgac aaaccggacc
      241 ttcgctatgg ctacgaactg accgacctga ccgaaatcct ccgcggcacc gaattcaagg
      301 ccttcggcgg cgtgatcgaa ggcggagggc aggtcaaggg catcaacttc ggccagcgcg
      361 agctttcccg ggcccagctc gacggcctga tcgacgacgc caaggagctc ggggccaagg
      421 gcctggtctg ggccttccgt gagggcgacg gctggcgggc cccgatcgcg aagttcctct
      481 ctgaaggcga actgaagaac ctgaacgagg cgatgggtgc cgaagaaggc gacctgatgc
      541 tggtcgccgc cgaccagccg gccatggtcg cggccatcct cggcggcctt cgcacccggc
      601 ttgccgagcg ctgggacctg atcccggccg atgccggcaa ccggttctgc tggatcgtcg
      661 actggccgct tttcgaattc aacgaggaag aagaccgctg ggatgccctg caccatccct
      721 tcaccgcgcc gaagggcgat ttcgatccgg aaaacccggg cgaggccagg gccgaggcct
      781 atgacctggt ctggaacggg gtcgagatcg gcggcggttc gatccgcatc aaccagccgg
      841 aactccagtc acagatcttc agggccctgg gtatctccga tgaggaagcc gaggaacgat
      901 tcggctttct gctcgaggcc ctccgatacg gggcaccgcc tcacggcggc atcgcctacg
      961 gggtcgatcg cttcgcggcg ctgctggccg gggtcgaatc gatccgtgac gtgatcgcct
     1021 tccccaagac cgcttccggc ggcgatccgc tgaccggcgc tccggccccg atcgacgacg
     1081 tccagatgcg cgacgtcggg atcgagcttc gcaagaagaa acttcccggg cagggttagg
     1141 cagcggggtc gcgcgatggg ttttctcgat cgcttcaggg acagggtgaa gtcgatcggg
     1201 gatggctcgg gccggatcca gatcaacgac cctcgtttcg acgactggga gaccgtccac
     1261 cagttcgagg atctggccac gggcctggcc tggcgggatg tcctgcgcga gcacggcatc
     1321 ccggccgagc tgacctccga ctgggagctc gatcgctacg gccggggaga catccacctc
     1381 caggtgccac ccggaatgtg gtccgatgcc gaagaactcg tgtccgggct tgacctggag
     1441 tgataggttg gggttgaggc tcgaacctgt tcgacagccg tctcgtaaat tgacccaaca
     1501 tcaacggcac gggagctgat ttcgatgtcc cccgcgggac gaagataggc acccaccttt
     1561 ttataaaggg atcaatgccg gaggcggcaa cggcaggttg gagcctcttt ttctatcttc
     1621 gcctgaactt gaccgccacc gcgaataggg tggacggcag acatcaagaa gaggagggga
     1681 aatgacctcg gttctacgga aggcagggct gaccggattg ctggccgccc ttgcactggc
     1741 aatcgctttg ccggcagcag cttcggctga cacccagcgc ttctacgaca actgggcagt
     1801 cgggtcgggt ccgatcggga tcgcaaacga ccggttcggg gatgtctacg tttccagcaa
     1861 cactccggcg ccgtttctct cccgatacac ggccgaaggc gagttcgtcg cctcgtttgc
     1921 gacgggcggg gccattctcg gagcaaggga cgtgacagta gattcggccg gaaacgtcta
     1981 ccaggtgaat gccaccaaca acatggtcag gaagttctcc ccggacggaa ccctgatcac
     2041 ggggtgggga accagcggat cgggtgacgg ccagtttctc gatccgaacg ccctggccac
     2101 gggaccggac aacaggatct atgtggggga ctttggcaac gatcgagtcc agtggttcac
     2161 gcccgcaggt gtgtacctcg ggcaattcgc cacgggcccc atggccggcc tcgatgtcgg
     2221 agccgatgga aacgtattcg tggccgattc gctgagcgat gaaatccggg tctacgaccc
     2281 gtccggaact ccgctcgaca ccgttggaga aaacggctcg ggcgcgggtg acatcgacaa
     2341 tgtgagcgac gtcgacatcg gcgacgacgg tcgcatctac gccgccgaca cttccagcgg
     2401 catgatcagg gtctatgcgc cggacgggtc ctatcagggc tcgctcggat ccccaggggc
     2461 tggcgacgga gagttgaacc ttcccaacgc acttaccgcg gaccgtgccg gcaatgtctg
     2521 gacgacgcag tacggcggat cgccccgggt cagcctgttc gcatacgcgc cgcgggtgat
     2581 cggaggtacg aacctcgact tcgggtattc cttcatcggt aaccctcccg gagtccagac
     2641 ggtgctgatg cagaacgaca attacgtcct gccgatgtat gtcgggacgg cgagcctgac
     2701 atccggggtc gacttcggac tcccggcacc ctggttcgag tgctctggcg tgatcttgct
     2761 cccgggccaa gtgtgcagcg tcggggtcga ctttcatccg gccacaccgg gaaacaagac
     2821 cgacacact
//
LOCUS       DAOQMF010000183         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20631_length_2824_cov_3.476345, whole genome shotgun sequence.
ACCESSION   DAOQMF010000183 DAOQMF010000000
VERSION     DAOQMF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20631_length_2824_cov_3.476345"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /gene="trxB"
                     /locus_tag="PLV77_04035"
     CDS             <1..903
                     /gene="trxB"
                     /locus_tag="PLV77_04035"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNC15046.1"
                     /translation="GPAGYTAALYAARAELNPLVFEGFQWGGQLQNTTDVENFPGFPE
                     GIMGPEMMTRFRDQAERFGTRFVTDDVDKVELTDGGIHKVYLGDEVHHAKTVILAMGA
                     EPKRLGIPGEMEMAGRGVSTCGVCDGAFFKDRSTMVIGGGDSAMEDAVFISKFASSLD
                     LVHRRDEFRASKIMQERAESLPNISLKTPWAPEEFVAGEDGALEKVRLKHSESGEVEE
                     IPVDGVFVAIGHIPRSELVVGQVDTDDEGYVKTEAASTKTNLAGVFAIGDLVDHTYRQ
                     AVTAAGSGCKGALDAEWYLRDATF"
     gene            complement(900..1637)
                     /locus_tag="PLV77_04040"
     CDS             complement(900..1637)
                     /locus_tag="PLV77_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC15047.1"
                     /translation="MSPRITLATGLRVLQQIRHDPRTIALLLLVPALLLTLMKYVFES
                     EPRVFQQIGAPLCGLFPFIVMFLVTSITMLRERTTGTLERLMTLPMSKLDLLLGYALA
                     FGLLAAAQALVICLVGFGLLGLNAPHGVLIIVGLAVLNALLGMSLGLLVSAFANTEFQ
                     AVQFMPAFIFPQLLLCGLFVARDQMAPLLEAVSWVMPFTWAYDALARAVSPQPLSGRV
                     AVDAAVVLAVTIGSLIFGAMTLRRRTA"
     gene            complement(1634..2359)
                     /locus_tag="PLV77_04045"
     CDS             complement(1634..2359)
                     /locus_tag="PLV77_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC15048.1"
                     /translation="MTATPAISIRDLRVSRGGNLVLPDISVEIEKGSVTGLLGPSGCG
                     KTTLMRSIVGVQIVAGGTVEVLGRPAGSSDLRNRVGYVTQAPSVYGDLSVGENLDYFA
                     RVVGVSPERVAETIETVGLSGFTGRLVSSLSGGQRARVSLASALLNKPDLLVLDEPTV
                     GLDPLLRAELWKTFHELAESGTTLLVSSHVMDEAGACDHLILIRDGRLLRHTTPEALR
                     RETGENDLGRAFLKVIESDEEAA"
     gene            complement(2356..>2824)
                     /locus_tag="PLV77_04050"
     CDS             complement(2356..>2824)
                     /locus_tag="PLV77_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNC15049.1"
                     /translation="TPTISLATVTYSPTVILNGVKSLSYAANMQVTRLARAAGADEAL
                     FVRPDGVVLELPTSTIFWATDDGRLRTPALEAGVLDSITRRKIVERLEVEQGEFPLED
                     LLAASEAFVASTTREAHPVSRVDDREMPLVDGEQTRAAAAAFAAALAEDLEAQ"
BASE COUNT          504 a          899 c          980 g          441 t
ORIGIN      
        1 gggccggccg ggtacacggc cgcgctctac gcggcccggg ccgagttgaa tccgctcgtc
       61 ttcgaaggtt tccagtgggg cggccagctc cagaacacga ccgacgtcga gaacttcccc
      121 ggttttcccg agggcatcat ggggccggag atgatgaccc gtttccggga tcaggccgaa
      181 cgcttcggca cccgtttcgt caccgatgac gtcgacaagg tcgagctcac cgacggtggc
      241 atccacaagg tctatctcgg tgacgaggtt catcacgcga agacggtcat cctcgcgatg
      301 ggcgccgagc cgaaacgcct cggcattccc ggcgagatgg agatggccgg ccgcggcgtc
      361 tcgacctgcg gggtctgcga cggagctttc ttcaaggacc gctcgaccat ggtgatcggt
      421 ggcggcgact cggcgatgga agacgcggtc ttcatctcca agttcgcatc gagcctcgac
      481 ctggttcatc gccgggatga gttccgtgcc tcgaagatca tgcaggagcg ggccgagagc
      541 ctgcccaaca tttccctgaa gaccccctgg gcgccggagg aattcgtcgc cggggaagac
      601 ggggccctgg agaaggttcg cctcaagcat tccgagagcg gcgaggtcga ggagattccg
      661 gtagacggcg tcttcgtggc gatcggccac atcccgcgtt ccgaactggt cgtcggccag
      721 gtcgacaccg atgacgaggg ttatgtgaag accgaggccg cctcgaccaa gaccaatctg
      781 gccggtgtct ttgcgatcgg cgacctggtt gaccacacct accgccaggc ggtcaccgcc
      841 gccggctccg gctgcaaggg cgccctcgac gccgagtggt acctccgcga cgcgactttc
      901 taggcagtcc gccggcggag ggtcatggcg ccgaaaatca gtgatccaat cgtcaccgcg
      961 agtacgaccg cggcgtcgac ggcgacccgg ccgctgagcg gctgagggga aacggcccgg
     1021 gccagggcgt cataggccca ggtgaagggc atgacccagg agaccgcttc gagcagaggc
     1081 gccatctggt cgcgggccac gaacagtccg cagagcagca gctggggaaa gatgaacgcc
     1141 ggcatgaact ggaccgcctg gaactcggtg ttcgcaaagg cgctgacgag cagcccgagc
     1201 gacatcccga gcagggcatt caggactgcc agcccgacga tgatcaggac gccgtggggg
     1261 gcgttcaggc ccaggaggcc gaatccgacc aggcagatga ccagcgcctg ggccgccgcc
     1321 agcagcccga aggcgagcgc gtagccgagc agcaggtcga gctttgacat cggcagggtc
     1381 atcagccgct caagcgttcc ggtggttcgt tcccgcagca tcgtgatcga ggtcaccagg
     1441 aacatgacga tgaagggaaa caggccgcag agcggagcgc cgatctgctg aaagaccctg
     1501 ggttcgcttt cgaacacgta cttcatcagg gtgaggagaa gggccggaac gagcagcagg
     1561 agggcgatcg tccggggatc gtgccggatc tgctgcagga ccctgaggcc ggtggccagg
     1621 gtgatgcgcg gactcacgcc gcctcctcgt ccgactcgat caccttgagg aaggcgcggc
     1681 cgaggtcgtt ctcgccggtt tcccggcgca gtgcttcggg cgtcgtgtgg cgcagcaggc
     1741 gtccgtcgcg aatcagaatc aggtgatcgc aagcgccggc ctcgtccatg acgtgactgg
     1801 aaaccagcag ggtggtgcca ctttcggcca gttcatggaa ggtcttccag agttcggccc
     1861 ggagcagggg atcgaggccg accgtgggct cgtcgagcac cagcagatcg ggtttgttca
     1921 ggagagcgct ggccagtgaa acccgggccc gttgccctcc cgagagcgag gagaccagcc
     1981 ttccggtgaa cccggagagg ccgaccgttt cgatggtctc cgcgacccgt tccggggaga
     2041 ccccgaccac tctcgcgaag tagtcgagat tctcgcccac ggaaaggtcc ccgtagaccg
     2101 agggcgcctg ggtcacgtaa ccgacccggt tgcgcaggtc actggacccg gccggccggc
     2161 cgagcacttc aaccgtgccc ccggccacga tctggactcc cacgatcgaa cgcatcaggg
     2221 tcgtctttcc acatccgctc gggccgagca gcccggtcac ggatcccttc tcgatctcga
     2281 ccgagatgtc cggcaggacg aggttccccc cgcgcgaaac gcgcaggtcg cggatgctga
     2341 tggccggcgt tgccgtcatt gagcttcgag gtcttcggcc agggcggcgg cgaatgccgc
     2401 ggcggcggcc ctcgtctgct cgccgtcaac gagcggcatt tcccggtcgt cgacccggga
     2461 gaccgggtgg gcctcgcggg tggtcgaggc gacgaaggct tccgaagcag cgagcaggtc
     2521 ttccaggggg aactcgccct gctcgacttc gagccgctcg acgatcttgc ggcgggtgat
     2581 cgagtcgagc acccccgctt ccagggccgg cgtacgcagc cgtccgtcgt cggttgccca
     2641 gaagatcgtc gaagtcggca gctcgagaac gaccccgtcc gggcggacga aaagcgcctc
     2701 gtccgctccg gccgcccggg ccagccgggt cacctgcatg ttggcggcgt aggaaagcga
     2761 cttgaccccg ttgaggatca cggtgggtga ataggtgacg gtggccagcg agatggtcgg
     2821 ggtg
//
LOCUS       DAOQMF010000184         2817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20707_length_2817_cov_3.510500, whole genome shotgun sequence.
ACCESSION   DAOQMF010000184 DAOQMF010000000
VERSION     DAOQMF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20707_length_2817_cov_3.510500"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /locus_tag="PLV77_04055"
     CDS             <1..2140
                     /locus_tag="PLV77_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC15050.1"
                     /translation="GETPVEIFRRLVIGSEGTLAFVASVTFDTVELPVLTTTAWLHFP
                     DIQTAVDPVPELVAAGATAVELMVAPALITAGWNMPGAPEYFRELDPESAALLVEFGG
                     PDEASLDAQEARALEIVDAEKLILPASFGRDEESIEINWKVREGLHGLIGKMRLPGTA
                     LIIEDVCVPPARIAEAAVELRSMLAEHGFLAGVAGHASAGNLHFMLTPDFSKPEDTER
                     YETFMNALVDLIIDKYDGALKAEHGTGINMAPYVIREWGETAVDFMWRAKQLADPENA
                     LNPGSILTRDRQAHLQNLKTTPPVEEIANACVECGFCEPVCPSRHLTTTPRQRIVLRR
                     EMVRQGEDTPVRRALLQEFEYEAIETCAADGSCLLACPVGIDTGKFIKGFRAEQHSPA
                     AEKMALRAARRWDRIEKLARTGLKLGGAVSSVASDGAMRGLTRAARKAMSADLVPEWG
                     EDMPPAAQPKLPQTTSHGAAAVYMPACINRMFGRSKDDPGKSLPEAMVEVSARAGKPV
                     WIPPDIAGTCCSVPWSSKGYRSAHEFKANQTVERIWRWTGGGTLPLVIDASSCGHGLI
                     DFGEGVLSEENAARHAELKIIDSVQWAEEFLLPGLEVTNKAESVTIHPTCSGRHLGLE
                     RSLTRIADALAERVEIPNVATCCGFAGDRGLLHPELTASATAAEAAEVKSEPTAKYIS
                     SNRTCEVGLERATGEPYGSFIYLLEEATRP"
     gene            complement(2170..2406)
                     /locus_tag="PLV77_04060"
     CDS             complement(2170..2406)
                     /locus_tag="PLV77_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15051.1"
                     /translation="MSLDPEYVVRIAKLEQKVSELYRHLGVAEPSGNEPLSPEVQALL
                     QQNNTIEAIKVHRDQTGLDMAAAKAAIDEYLNQG"
     gene            2503..>2817
                     /locus_tag="PLV77_04065"
     CDS             2503..>2817
                     /locus_tag="PLV77_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNC15052.1"
                     /translation="MKKLVLAVTTAFLAICCCAPMAAAAPDRDPIPPPTDYWQDKGFI
                     LMAHQGGEWDFPPNTLFAYKSAMAIGADMLDMDAYVTRDDHIVLTHDLDARSNSNLTD
                     DMF"
BASE COUNT          488 a          986 c          910 g          433 t
ORIGIN      
        1 cggcgagaca ccggtcgaga tcttccgccg tctggtcatc ggttcggaag gcaccctcgc
       61 cttcgtcgcc agcgtcacct tcgacacggt cgagttgccg gtgctgacca ccaccgcctg
      121 gctccacttc cccgacatcc agaccgcggt cgacccggtt cccgagctgg tcgcggcggg
      181 ggccaccgcg gtcgagctga tggtcgcccc ggccctgatc acggccggct ggaacatgcc
      241 cggcgccccc gagtacttcc gcgagctcga tccggaatcg gccgcactgc tggtcgagtt
      301 cggcggaccc gacgaggcca gccttgatgc ccaggaagcc agggcgctgg agatcgtcga
      361 tgcggagaag ctgatcctgc cggccagctt cggccgggac gaggaatcga tcgaaatcaa
      421 ctggaaggtc cgcgagggcc tccacggcct gatcggcaag atgcgcctgc ccggcaccgc
      481 gctgataatc gaggacgtct gcgtgccgcc cgcccggatc gccgaggccg cagtggagct
      541 gcgcagcatg ctcgccgaac acggcttcct cgccggcgtc gccggtcacg cctcggccgg
      601 caacctgcac ttcatgctca ccccggactt ctcgaagccg gaagacacgg agcgctacga
      661 gaccttcatg aacgccctgg tcgatctgat catcgacaag tacgacggag cgctcaaggc
      721 cgagcacggc accgggatca acatggctcc gtacgtgatc agggagtggg gcgaaacggc
      781 ggtcgacttc atgtggcggg ccaagcagct ggccgacccg gagaacgcgc tgaatcccgg
      841 ctcgatcctg acccgggaca ggcaggccca cctgcagaac ctgaagacga ccccgccggt
      901 cgaggagatt gccaacgcct gcgtcgaatg tggtttctgc gagccggtct gcccgagccg
      961 ccacctgacc accaccccgc ggcaacgcat cgttctgcgc cgggagatgg tccgccaggg
     1021 cgaggacacc ccggtccgcc gggccctgct ccaggagttc gagtacgagg cgatcgagac
     1081 ctgcgccgcg gacggttcct gcctgctggc ctgcccggtc gggatcgaca ccggcaagtt
     1141 catcaagggt ttccgggccg agcagcacag tcccgcggcc gagaagatgg ccctgcgggc
     1201 cgcccgtcgc tgggaccgga tcgagaagct ggcccgtacc ggcctcaagc tcgggggtgc
     1261 cgtttccagc gtcgcttcgg acggggcgat gcggggcctg acccgcgccg cacgcaaggc
     1321 gatgagcgcg gacctggttc ccgaatgggg cgaggacatg ccgccggccg cccagccgaa
     1381 gctgccacag acgacctccc atggcgccgc cgccgtgtac atgccggcct gcatcaaccg
     1441 catgttcggc cgctccaagg atgacccggg gaagagcctg cccgaggcaa tggtcgaggt
     1501 ctcggcccgg gccggcaagc cggtctggat cccgccggac atcgccggca cctgctgttc
     1561 ggttccgtgg agctcgaagg gctacaggag cgcccacgag ttcaaggcga accagacggt
     1621 cgagcggatc tggcgctgga ccggcggggg aaccctgccg ctggtgatcg acgcctcgtc
     1681 ctgcggccac ggcctgatcg atttcggcga gggggtgctg agcgaggaga acgccgcccg
     1741 ccacgccgag ctgaagatca tcgactccgt ccaatgggcc gaagagttcc tgctaccggg
     1801 cctcgaagta accaacaagg ccgaatcggt cacgatccat ccgacctgct cgggccgcca
     1861 cctgggtctg gaaaggtccc tgacccggat cgccgacgcc ctcgcggagc gggttgaaat
     1921 cccgaacgtc gccacctgct gcggcttcgc cggtgaccgc ggcctgctcc atccggaact
     1981 gaccgcaagc gccaccgccg ccgaggcggc cgaggtcaag tcggaaccga ccgcgaagta
     2041 catctcctcc aaccggacct gcgaggtcgg cctcgaacgc gcgaccggcg aaccctacgg
     2101 ctccttcatc tacctcctcg aagaagccac ccggccctga ccgggacccc ggggtcaggc
     2161 ccggaggagc tagccctggt tcaggtactc gtcgattgcg gctttggcgg cggccatatc
     2221 gaggccggtc tggtcgcggt ggaccttgat cgcctcgatc gtgttgttct gctggagcag
     2281 agcctgaacc tcgggtgaga gcggctcgtt gccggacggc tcggcaaccc ccagatgccg
     2341 gtagagctca ctcaccttct gctcgagctt ggctatgcgg acgacgtatt caggatccag
     2401 actcatgctc cgaccctaac tccccggtca cctttccgtg acaattttcc ggatgcgggc
     2461 actacactct ggcgtgggag aaacgaaaca ggggaatcga tgatgaagaa gctggtcctg
     2521 gccgtcacaa ccgcttttct ggcgatctgc tgctgcgccc cgatggctgc ggcggcgccg
     2581 gaccgggatc cgatcccgcc gccgaccgac tactggcagg acaagggctt catcctgatg
     2641 gcccatcagg gtggcgagtg ggacttcccg cccaacaccc tgttcgccta caagtccgcg
     2701 atggcgatcg gcgccgacat gctcgacatg gacgcctacg tgacccgcga cgatcacatc
     2761 gtccttactc acgacctcga cgcccgcagc aactcgaacc tcaccgacga catgttc
//
LOCUS       DAOQMF010000185         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20734_length_2815_cov_3.301087, whole genome shotgun sequence.
ACCESSION   DAOQMF010000185 DAOQMF010000000
VERSION     DAOQMF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20734_length_2815_cov_3.301087"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="rplC"
                     /locus_tag="PLV77_04070"
     CDS             complement(<1..325)
                     /gene="rplC"
                     /locus_tag="PLV77_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577044.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNC15053.1"
                     /translation="MTQLFREDGEVVPVTVIEAGPCPVTAIRNEERDGYSAIQLAFDE
                     VKESKLTKAELGHLAKADAAPSRVLVEFRDVELPAAEGEEGEEGDGEVKLGQKVTVAA
                     FEEGQK"
     gene            complement(361..672)
                     /gene="rpsJ"
                     /locus_tag="PLV77_04075"
     CDS             complement(361..672)
                     /gene="rpsJ"
                     /locus_tag="PLV77_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNC15054.1"
                     /translation="MAAQKIRIRLKAYDHDAIDRAAREIVDTAERTGATVSGPVPLPT
                     ERNIYCVIRSPFKDKDSREHFEIRTHKRLIDIKQPTPKTVDSLQRLDSLPAGVDIEIR
                     L"
     gene            complement(978..2153)
                     /gene="tuf"
                     /locus_tag="PLV77_04080"
     CDS             complement(978..2153)
                     /gene="tuf"
                     /locus_tag="PLV77_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNC15055.1"
                     /translation="MSKEKFERDKPHVNVGTIGHVDHGKTTLTAAITNTLSEDKKSFE
                     EIDNAPEEKERGITIATSHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVV
                     SAADGVMPQTREHILLARQVNVPHVVVFLNKVDQVDDEELIELVEEEVRDLLNEYEFD
                     GDNTPIIKGSALKALEGDADFQARIVELGEALDSYIPEPERDLDKPFLMPIEDVFSIT
                     GRGTVATGRIEQGIVNTGDEVEIVGIHDKTETTTITGVEMFRKILDEGRAGDNVGCLL
                     RGTKREEIERGQVLCKPGSITPHTKFRAEVYCLKKEEGGRHTPFFSGYRPQFYFRTTD
                     VTGVANLPEGTEMVMPGDNVEMTIELIQPIAMDQGLRFAIREGGRTVGSGVVSEIIE"
     gene            complement(2302..>2815)
                     /gene="fusA"
                     /locus_tag="PLV77_04085"
     CDS             complement(2302..>2815)
                     /gene="fusA"
                     /locus_tag="PLV77_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932817.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNC15056.1"
                     /translation="GGVIPTEYIPAVQQGIKEALENGVKAGYPMVDVKVELIDGSYHD
                     VDSSEMAFKIAGSMALQEAARKASPTLLEPVMAVEVTTPEDFLGDVIGDLSRRRGKVQ
                     GQEQRGNALAVQAYVPLSEMFGYATDLRSSTQGRAQYTMQFERYEEVPSNIAEEIAES
                     RSGAKAGAEA"
BASE COUNT          431 a          859 c          905 g          620 t
ORIGIN      
        1 ccttctggcc ttcctcgaag gccgcgacgg tgaccttctg gccgagcttg acctcgccgt
       61 cgccttcctc accctcttcg ccttcggcgg cagggagttc gacgtcgcgg aactcgacga
      121 gcacacgcga aggggcggcg tccgccttgg ccaggtgacc gagttcggcc ttggtcagct
      181 tcgactcctt gacttcgtcg aaagcgagct gaatcgccga gtagccgtcg cgctcctcgt
      241 tgcggatcgc ggtaaccgga cagggtccgg cttcaatgac tgttacgggc acgacctcac
      301 cgtcttctct gaacagttgg gtcatgccaa gcttttttcc gagaatcgcc gacatggtgc
      361 ctacagtcgg atctcgatgt cgacgccggc cgggagcgag tccaggcgct gcagcgaatc
      421 gacggtcttg ggggtgggct gcttgatgtc gatcagacgc ttgtgggtgc ggatctcgaa
      481 gtgctcccga gagtccttgt ccttgaacgg cgaacggatc acgcagtaga tgttgcgctc
      541 ggtcggcagc ggcacgggac cggaaacggt cgcgccggtg cgctcggccg tatcgacgat
      601 ctcgcgggca gcgcgatcga tggcgtcgtg atcgtaggcc ttgagcctga tcctgatttt
      661 ctgtgcggca atagcggcca ctttggcttc tcttcttact ttcttctgct ggcgcaaatc
      721 agctgtatcg gctgaggcgc tgatgtcaag gtcccccgcc gcctgcggct cagtccccgc
      781 ccgtgaggcg gatccagtgc cgtggttcga caatccgggg tacttcaaag gtcgtatctg
      841 ttactggtgc tgcgaacttc tttacttcgt tactgcgtta ctgctgtgct gcggtacttc
      901 aggccctgag ggcgcaaaaa cggggcggac ccgaaaacgt ttgctgtctt cgggtccgcc
      961 ccgggcaaat cgtgtcgcta ctcgatgatc tccgagacga cgccggagcc gaccgtgcgg
     1021 ccaccctcgc ggatcgcgaa gcggaggccc tggtccatgg cgatcggctg gatcagctcg
     1081 atcgtcatct cgacgttgtc acccggcatg accatctccg tgccctcggg caggttggcg
     1141 acgccggtca cgtcggtggt ccggaagtag aactgcgggc ggtagcccga gaagaacggg
     1201 gtgtgacgtc cgccttcttc cttcttcagg cagtaaacct cggcccggaa cttggtgtgc
     1261 ggggtgatcg agcccggctt gcagagaacc tggccgcgct cgatttcctc acgcttggtg
     1321 ccacggagca ggcagccgac gttgtcgcca gcgcgaccct cgtcgaggat cttgcggaac
     1381 atctcgacgc cggtgatcgt ggtggtctcg gtcttgtcgt gaataccgac gatctcgacc
     1441 tcgtcaccgg tgttgacgat gccctgctcg atacggccgg tggcgacggt gccgcggccg
     1501 gtgatcgaga agacgtcctc gatcggcatc aggaacggct tgtcgaggtc acgctccggc
     1561 tccgggatgt aggagtccag cgcctcgccc agctcgacga tcctggcctg gaagtcagcg
     1621 tcgccttcga gggccttgag agccgaaccc ttgatgatcg gggtgttgtc accgtcgaac
     1681 tcgtactcgt tgaggaggtc gcggacctct tcctcgacca gctcgatgag ctcctcgtcg
     1741 tcgacctggt cgaccttgtt gaggaacacg acgacgtgcg gcacgttgac ctggcgggca
     1801 agcaggatgt gctcgcgggt ctgcggcatg acaccgtcag cggcagaaac gaccaggatc
     1861 gcgccgtcca tctgggcggc accggtgatc atgttcttga cgtagtcggc gtggccgggg
     1921 cagtcgacgt gggcgtagtg ccggttcggg gtctcgtact cgacgtgaga ggtcgcgatg
     1981 gtgatcccgc gctccttctc ttccggagcg ttgtcgatct cctcgaagct cttcttgtct
     2041 tcggagagag tgttggtgat ggccgcggta agggtggtct tgccgtggtc gacgtgaccg
     2101 atggtgccga cgtttacgtg cggcttatcg cgctcgaact tctccttgga catgctctcc
     2161 tgggaccttt ctcgtctgtt tctcttggat cttggtttct acgtctgatt gaactgctaa
     2221 agcttggtgg ctccggactt tcgcccattt tgggcgaacc tctgaaatat agccatttag
     2281 ccccggcggg gtatttacgg cctaggcttc tgccccggcc tttgcgcctg aacggctctc
     2341 ggcgatctct tcggcgatgt tgctcggcac ctcttcgtag cgctcgaact gcatcgtgta
     2401 ctgggcccgg ccctgggtcg aagaccgcag gtcggtcgcg taaccgaaca tctcgctaag
     2461 cggcacgtaa gcctgaacgg cgagcgcgtt gccgcgctgc tcctggccct ggaccttgcc
     2521 gcgacggcgc gaaaggtcgc cgatcacgtc gccgaggaag tcctcgggag tggtgacctc
     2581 gaccgccatg accggctcga gcagggtcgg gctggccttg cgggcagctt cctgaagcgc
     2641 catcgaaccg gcgatcttga aagccatttc cgaggagtcg acgtcatggt aggagccgtc
     2701 gatcagctcg accttcacgt caaccatcgg gtagccggcc ttgacgccgt tctcgagcgc
     2761 ttccttgatt ccctgctgga cggccgggat gtactcggtc ggaatcacgc cgccc
//
LOCUS       DAOQMF010000186         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_20864_length_2805_cov_3.165091, whole genome shotgun sequence.
ACCESSION   DAOQMF010000186 DAOQMF010000000
VERSION     DAOQMF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20864_length_2805_cov_3.165091"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..1181)
                     /locus_tag="PLV77_04090"
     CDS             complement(315..1181)
                     /locus_tag="PLV77_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15057.1"
                     /translation="MAQIKALLFNDPSCPWGYSASPALRTLEWRYRDQIEWHLAVIGL
                     SDENHPVPYPPETLSDLYFDFRCRYGMPFAIETKIRPFTSATSCKAITAARLLYPGSE
                     WRVLRTLQLLHFNTPLLLDDRTQLGEALGTVPGLDASTVMTAIDSPEVAAAYRTDYDH
                     ARSATGSPTHLQAKTASHSTGERYTAPSVIFEYGDKRAEVGGFQPVEAYDVMIANIEP
                     GLKRMGPAEDPLEALGLYPAGLTTQEVAAIMASGMDKPERVDAERKLVHLLGERKVKR
                     VPLGDDALWIKA"
     gene            1235..1549
                     /locus_tag="PLV77_04095"
     CDS             1235..1549
                     /locus_tag="PLV77_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15058.1"
                     /translation="MEADRNISPPRSAGLLFVAAIWALLLTLVGSPEVVFFTVPVFLL
                     AAPLAFGRYTGEGLIGALRGGRVRTDGRDLPRHLIDAARRAVTPVHFERRLGRAPPVS
                     VN"
     gene            1640..2362
                     /locus_tag="PLV77_04100"
     CDS             1640..2362
                     /locus_tag="PLV77_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
                     /protein_id="HNC15059.1"
                     /translation="MKRLPGILALLILALVPSMAMAHQGNPDYRSEITSVQPEALGEG
                     LTIEIVNFDDHVRLVNKTGKVVLIKGYDGEPYARVEADGKVFVNLNSPAYYLNEDRFA
                     AVEVPKRADAKAAPDWKQIADNGTFEWHDHRSHYMGTGTPSQVTDESKETKVFDYVIP
                     IEVGGEPAKINGTLTWVGTGSKAPVIPFVILGLAIIAAIVFWLMRRRRSEDDDGPREG
                     GSGEAGGSGDDNPDESGKSEAW"
     gene            2359..>2805
                     /locus_tag="PLV77_04105"
     CDS             2359..>2805
                     /locus_tag="PLV77_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNC15060.1"
                     /translation="MRRSLLTSLLFLACLLGLASSASAHAVLEGSSPTRGAELNSSPG
                     HVDFTFNEPVEASLGAVRVFNTSGDQVQTDDLERPGENADAVGASLPADLPDGLYTAT
                     YHVISADSHPVSGGITFTIGKPGKDASAFTQGKTISELLAESEAGKV"
BASE COUNT          490 a          874 c          927 g          514 t
ORIGIN      
        1 agaagttcct gatcgagacc ttgccggtct tctcgatctc gcgcacgacc agcccggtga
       61 tcaggaaccc ggagagaacg tagaagacgt cgaccccgat gaaaccgccc tcggcccagg
      121 ggatcgaggc atggcatgca agcacgaggg ttaccgcgat tgcgcggatt ccctggatat
      181 cgggccggaa gttcgaaccc gcttgttttg ccgtggggga gccgctttct ctggcggcga
      241 cctcggcacc gccaagagcc atcggcgcag gctacaggtt tggggtgacg ccccgctcgc
      301 tcagcgcggg tacctcaagc cttgatccag agggcgtcgt cgcccagcgg aacccgtttc
      361 acttttcgct ctccgagcag gtgaaccagc tttcgttcgg cgtccacgcg ctcgggcttg
      421 tccatcccgg aagccatgat cgctgcgacc tcctgagtgg tcaggccggc cgggtaaagt
      481 ccgagcgcct ccagcggatc ctcggccgga cccatgcgtt tcaggccggg ttcgatgttg
      541 gcgatcatca cgtcgtaggc ctccactggc tggaagccgc ccacttcggc tcgcttgtcg
      601 ccgtattcga agatcaccga gggtgccgta tacctttcgc cggtcgagtg ggaagcggtc
      661 ttggcctgga ggtgggtcgg actcccggtc gcgctgcggg cgtggtcgta atcggtccgg
      721 taggcggcgg ccacttccgg gctgtcgatc gccgtcataa ccgtcgacgc gtctagcccg
      781 gggaccgttc ccagtgcctc gcccagctgg gtccggtcat cgagcaggag gggagtgttg
      841 aagtggagca gctgaagggt gcggaggacc cgccattccg aacccggata gagcagtcgg
      901 gcggcggtga tggccttgca ggacgtggcg ctggtgaaag ggcggatctt tgtctcgatc
      961 gcaaaaggca tgccgtagcg gcagcggaaa tcgaagtaga ggtcggagag agtctccggc
     1021 gggtagggta cgggatggtt ctcgtcagaa aggccaatca ctgcgagatg ccactcgatc
     1081 tggtcgcggt agcgccactc aagagtcctc agggcgggac tggccgagta gccccaggga
     1141 caggacgggt cattgaagag aagtgccttg atctgcgcca tgccgcgaag cctagccact
     1201 ccttttggtc gactcgcggg ttaggattca tgtgatggaa gcggaccgga atatttcgcc
     1261 tccccggagc gccgggttgc tgttcgttgc cgcgatctgg gcgctgctcc tgaccttggt
     1321 cggctccccc gaagtggttt tcttcacggt tcccgtcttc ctgctcgccg caccgctcgc
     1381 cttcggccgg tacacagggg aaggtctgat cggggctctg cgcggaggtc gggtccggac
     1441 cgacggccgc gacctgccgc ggcatctgat cgacgccgcc cggagggcgg tcacgccagt
     1501 gcatttcgag cggcggcttg gccgggcgcc acctgtttcc gtgaactgag ccggccggta
     1561 tcgggccggc tcgcataatc cgacccgacc ccgcggcaag tagccgcacc gactcgcaat
     1621 ctgcggttat ggagtgacca tgaagcgcct cccaggcatc cttgctttgc tcatacttgc
     1681 cctcgttcct tcgatggcga tggcccacca gggcaatccc gactaccggt ccgagatcac
     1741 ctcggtccag ccggaagcgc tgggcgaggg actcacgatc gagatcgtga acttcgatga
     1801 ccatgttcgc ctggtcaaca agaccggaaa ggttgttctg atcaagggat atgacggtga
     1861 gccttacgcc cgggtcgagg ccgacggcaa ggttttcgtg aacctgaatt cgccggccta
     1921 ctacctcaac gaggaccggt tcgcggcggt ggaagtgccg aagcgggccg atgcgaaagc
     1981 cgctcccgac tggaagcaga tcgccgacaa cggcaccttc gagtggcatg accaccgctc
     2041 gcattacatg ggcacgggaa cgccctcgca ggtgaccgac gagtcgaagg agaccaaggt
     2101 cttcgactat gtgatcccga tcgaggtcgg tggcgagccg gcgaagatca acggcaccct
     2161 gacctgggtc ggtaccggat cgaaagcgcc ggtgatcccc ttcgtgatcc tcgggctggc
     2221 gatcatcgcc gcgatcgtct tctggttgat gcggcgacgc cgcagcgaag atgatgacgg
     2281 acctcgcgaa ggcggatccg gggaagcagg tggatcgggc gatgacaacc cggatgaatc
     2341 aggcaagagt gaggcctggt gaggcgcagc ctcctcacaa gtctgctttt cctggcctgc
     2401 ctgctcgggc tggccagttc ggcctctgcc catgccgtgc tcgagggttc atccccgact
     2461 cggggggcgg agctcaactc ctcacccggc cacgtggact tcaccttcaa cgagccggtc
     2521 gaagcgtcgc tgggtgcggt ccgggtcttc aacacatccg gtgaccaggt ccagaccgat
     2581 gacctcgagc gtccgggtga aaacgcggac gcggtcggag ccagcctgcc cgcggacctg
     2641 cccgacgggc tttacaccgc gacctaccat gtgatctcgg ccgattccca cccggtttcg
     2701 ggcggcatca ctttcacgat cggcaaaccc ggcaaggacg catccgcctt cacccagggc
     2761 aagacgattt cggaactcct ggccgaaagc gaggcgggca aggtc
//
LOCUS       DAOQMF010000187         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21038_length_2792_cov_3.027768, whole genome shotgun sequence.
ACCESSION   DAOQMF010000187 DAOQMF010000000
VERSION     DAOQMF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21038_length_2792_cov_3.027768"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLV77_04110"
     CDS             complement(<1..1073)
                     /locus_tag="PLV77_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15061.1"
                     /translation="MKRFNLLLIVAGLAAMLLAMPMSASAGKKPVDKATSGLKDTLKT
                     NVRIISTSQGPVRSSGQLKVKVSVKASKSTPRDIKKNPIKIALHGSAKVIWSPRSNVS
                     LYGETLKLKPNHSRVVKLKLNPTGKSKAGDCGAPTFEVTATYRSGKRKKTAKSSRKLA
                     PQKSLCDVPAGVDLASSDKCDFIAGPGNACLAPFPNDFYTREDEGSTTGKRLDLDPDS
                     TPANSSEGKHIAVNVLNQSDGFSPGPLLSVRIPGMDNQAAFDQSGIVSIKEMSAAYAP
                     AQPVVLIDAATGERQLIWAELDSQATTDADRNLIIRPAKNLTNGHRYIVAIRNMKDSS
                     GDPIQAPAAFRLYRDTDRTGDNR"
     gene            1338..>2792
                     /locus_tag="PLV77_04115"
     CDS             1338..>2792
                     /locus_tag="PLV77_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC15062.1"
                     /translation="MAFGVAAGQAQAKPSIAAQAKKICKKKRGKAKAKCVKKQVKRLK
                     AKARRERLKELNRPGVTIRTTEYGIPRITADSWRGLGYGYGYALANENICSMADIYTT
                     VRGERSKYFGPTGTWMLTGNGIQYTNLESDFSHKLVIAQNTIPKILKMKPPNGPLPQV
                     REVIDGYVKGYNAWLTKTGVNKIQDSTCKGKPWVKKITPLDAYLRFYELATMAGSGAA
                     VDGIANAAPPAETRTGAPSGSGDSNVTAADFKALNKPAAIGSNAVSLGSQATDTGKGM
                     LFGNPHFPWSGSERFFESQLTIPGKINVSGASLLGSPVIQIGHTQNMAWSHTVSTARR
                     FAFFRESLDPSDPTRYMVDGVSTPMKSNTITVDKGNGDTETRTLYSTKHGQISTSIQK
                     TPLFGWDSNYAYALFDANANSLRVVNQFFSFNTAESVAGMKKALETYQGVPWVNTIAA
                     DSKGNAMNADVSVVPNLTDARVAECNTPGLGEVAW"
BASE COUNT          527 a          872 c          895 g          498 t
ORIGIN      
        1 atcctgttgt cgccggtgcg gtcggtgtcg cggtacagcc ggaacgcggc cggagcctgg
       61 atcgggtcac cggaggaatc cttcatgttg cggatcgcga cgatgtaccg gtggccgttc
      121 gtcaggttct tcgcgggccg gatgatcaga ttgcgatccg catccgtggt cgcctgactg
      181 tcgagctcgg cccagatcag ctggcgctcg ccggtcgccg cgtcgatcag cacaaccggc
      241 tgggcggggg cgtaggcggc gctcatctcc ttgatcgaaa cgatcccgga ctggtcgaag
      301 gcggcctggt tgtccatgcc ggggatccgc accgaaagca gcggaccggg gctgaacccg
      361 tcggactgat tcaacacgtt cacggcgatg tgcttgccct cggaggagtt cgccggcgtg
      421 gaatcggggt cgaggtcgag ccgctttccg gtggtcgagc cctcgtcctc cctggtgtag
      481 aagtcgttcg ggaagggggc cagacaggca ttgcccggtc cggcgatgaa gtcacacttg
      541 tcggaactgg cgaggtcaac gccagccggc acgtcgcaga gcgacttctg gggggccagc
      601 ttgcggctgg actttgcggt cttcttgcgc ttgccggaac ggtaggtcgc ggtcacctcg
      661 aaggtcgggg ccccgcaatc gccggccttg gacttgccgg tcgggttcag cttcagtttc
      721 accaccctgg agtggttggg cttgagcttc agcgtctcgc cgtacagcga gacgttcgaa
      781 cggggggacc agatgacctt ggcgctgcca tgaagcgcga tcttgatcgg gttcttcttg
      841 atgtcccgag gcgtcgattt cgaagctttg accgagacct tgaccttgag ctggccgctc
      901 gagcggaccg gtccctggct ggtactgatg atccggacgt tggtcttgag cgtgtccttg
      961 agaccgctgg tcgccttgtc caccggcttc ttcccggccg acgcggacat cggcatcgcc
     1021 agcagcatcg ccgccagtcc cgccacgatc aacagaagat tgaatcgctt cacctgactc
     1081 cccttttccc tctcggtttt ctccccgggt cgaatgaccc tttccgaaga atagcgccga
     1141 tcgggcgaca aaccgggaaa cggaagcggt ttcgggaaag cggccgtacc gtacgcggat
     1201 gtatgcttgg accgctccga agctctgttt tcggggttag agggattgga gaaatgaggg
     1261 gccgcagggg cgtctcgtca aagactggag agaacttgaa gtccatcaga cttttggttg
     1321 cagccattgt catcttgatg gcattcggtg ttgcagcagg acaggcacag gccaagccga
     1381 gcattgcggc gcaggcgaag aagatctgca agaagaagcg cggcaaggcg aaggccaagt
     1441 gcgtgaagaa gcaggtcaag cggctcaagg cgaaggccag gcgggaacgg ctcaaggagc
     1501 tgaaccgccc cggcgtcacg atccggacca ccgagtacgg gattccgcgc atcaccgctg
     1561 acagctggcg cggcctcggt tatggctacg gctacgccct ggccaacgag aacatctgct
     1621 cgatggcgga tatctacacc acggtgcgcg gcgagcgctc gaagtacttc ggcccgaccg
     1681 gcacctggat gctgaccgga aacgggattc agtacacgaa cctcgagtcg gatttctcgc
     1741 acaagctggt catcgcccag aacacgatcc ccaaaatcct gaagatgaag ccgccgaatg
     1801 gtccgctgcc gcaggtcagg gaagtgatcg acggttacgt caagggctac aacgcgtggc
     1861 tgaccaagac cggcgtcaac aagatccagg acagcacctg caagggcaag ccgtgggtga
     1921 agaagatcac cccgctcgac gcctacctgc ggttctacga gctcgccacc atggccggct
     1981 ccggcgccgc ggtcgacggc atcgccaatg cggccccgcc ggccgagacg cgtaccggcg
     2041 ccccgtccgg ttccggtgac tccaacgtca ccgccgccga cttcaaggcg ctcaacaagc
     2101 ccgccgcgat cggctcgaac gccgtttcgc tgggttcgca ggcaaccgat accggcaagg
     2161 gcatgctctt cggcaacccc cacttcccgt ggtccggttc cgagcggttc ttcgaatcgc
     2221 agctgaccat ccccggcaag atcaacgtct ccggcgccag cctgctcggc agcccggtga
     2281 tccagatcgg ccacacgcag aacatggcct ggagccacac ggtctcgacc gcgcggcgct
     2341 tcgccttctt ccgcgagtcg ctggacccga gtgatccgac caggtacatg gtcgacgggg
     2401 tttcgacccc gatgaagtcg aacacgatca cggttgacaa gggcaacggg gacaccgaga
     2461 cccgcaccct ctactcgacc aagcacggac agatctcgac ttcgatccag aagaccccgc
     2521 tgttcggctg ggacagcaac tacgcctacg cgctgttcga cgcgaatgcg aacagccttc
     2581 gggtcgtcaa ccagttcttc agtttcaaca ctgcggaaag cgttgccggc atgaagaagg
     2641 cgctggagac ctaccagggc gtgccgtggg tcaacacgat cgcggccgac tccaagggca
     2701 acgcgatgaa cgccgacgtt tcggtcgtgc cgaacctgac cgacgcgcgg gttgcggaat
     2761 gcaacacgcc tggcctgggt gaagtcgcct gg
//
LOCUS       DAOQMF010000188         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21326_length_2774_cov_3.446855, whole genome shotgun sequence.
ACCESSION   DAOQMF010000188 DAOQMF010000000
VERSION     DAOQMF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21326_length_2774_cov_3.446855"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLV77_04120"
     CDS             complement(<1..686)
                     /locus_tag="PLV77_04120"
                     /inference="COORDINATES: protein motif:HMM:NF036723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HNC15063.1"
                     /translation="MLETAEAPQHETHTVFNQSTPLENVNLYEIDLPLQEAVNREGAG
                     WAEDRIKALGEICGQPEVLLAGRDANEFPPRLKAFDRFGNRIDEVEFHPAWHELMALG
                     IEHDLHALPWREPGIGANVARAALFTQLTQVEAGVGCPISMTFSVIPSLRNQPEVAEE
                     WEPRFTSLEYDGKLKPADQKPGCLAGMAMTEKQGGSDVRANTTRAVPLNGGGPGGEYE
                     ITGHKWFCSAP"
     gene            817..2031
                     /locus_tag="PLV77_04125"
     CDS             817..2031
                     /locus_tag="PLV77_04125"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNC15064.1"
                     /translation="MSKIKVTNPIVELDGDEMTRIIWSFIKEQLILPYLDVDLKYYDL
                     GIESRDDTDDQITVDAANAIKEHGVGVKCATITPDEARVEEFDLKEMYRSPNGTIRNI
                     LGGVIFREPIVISNVPRLVPGWTKPIIIGRHAFGDQYRATDMVVEGEGTLTMTFTPKD
                     EGEPIEMHVHDFPGGGVAMAMYNHDDSIRDFARASFRYGLERGYPVYMSTKNTIMKRY
                     DGRFMILFQEVFDDEFKDEFDQAGLTYEHRLIDDMVASALKWEGGYVWACKNYDGDVQ
                     SDTVAQGFGSLGLMPSVLMTPDGKTVEAEAAHGTVTRHYREHQKGNPTSTNPIASIFA
                     WTRGIAARGRMDETPEVSQFGETLEKVCIETVESGKMTKDLALLVGPDQEWQTTQEFL
                     ASIDENLQKAMA"
     gene            2062..>2774
                     /locus_tag="PLV77_04130"
     CDS             2062..>2774
                     /locus_tag="PLV77_04130"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNC15065.1"
                     /translation="MSSTAKVTVTGAAGQIGYALLFRIASGQMLGPDTKVHLSLLEIP
                     AAVKAAEGTAMELDDCAFPLLEKISISDDPKEAFDGVNVGLLVGARPRGPGMERADLL
                     EANGGIFKPQGEAINAGAADDVKILVVGNPANTNALIAASAAPDVPKERFTAMMRLDH
                     NRAIAQLANKLGRSVSDVKRMTVWGNHSATQYPDIGNALVDGAPAPAQVDDEVWVREE
                     FIPRVAKRGAEVIDARGAS"
BASE COUNT          545 a          914 c          865 g          450 t
ORIGIN      
        1 ggggcggagc agaaccactt gtgaccggtg atctcgtatt cgccgccggg gccgccaccg
       61 ttcagcggaa ccgcgcgagt ggtgtttgcc cggacgtcgg agccaccctg cttctcggtc
      121 atcgccatcc cggccaggca gccaggcttc tggtctgccg gcttcagctt gccgtcgtac
      181 tcgagggagg tgaagcgggg ctcccattcc tcggcgactt caggctgatt tcgaagtgag
      241 gggatcaccg agaaggtcat cgagatcggg cagccgacgc cggcctctac ctgggtcagc
      301 tgggtgaaca gggcagcccg ggccacattg gcgccgattc ccggctctcg ccacggcagg
      361 gcatggaggt cgtgctcgat cccgagcgcc atcaactcgt gccatgccgg gtggaactcg
      421 acctcgtcga tccggttgcc aaaacggtcg aaagccttca gccggggcgg aaactcgttc
      481 gcatcccggc cggccagaag cacctcgggc tggccacaga tttcaccgag ggccttgatc
      541 cggtcctcgg cccagcctgc accctccctg ttgacggcct cctgaagcgg aaggtcgatc
      601 tcgtagaggt tgacgttctc cagcggagtc gactgattga agaccgtatg ggtctcgtgc
      661 tgcggagctt cggcggtttc cagcatggag taatggtctc acagccccgg cgcattgagg
      721 cacggataac tacccattga cgactgcaag gcgtttcgct ctatagcatc gctgcctgca
      781 agagccttac gccaaaatcg aagggaagta ccagtcgtgt cgaagatcaa agtgaccaac
      841 ccgatcgtcg agctggatgg cgacgagatg acccgcatca tctggtcgtt catcaaggag
      901 cagctgatcc ttccctatct cgacgtcgat ctgaagtact acgacctcgg gatcgaaagc
      961 cgcgacgaca ccgatgacca gatcacggtc gacgccgcca acgcgatcaa ggagcacggg
     1021 gtcggggtca agtgcgcgac catcactccg gacgaggccc gggtcgagga gttcgacctg
     1081 aaagagatgt accgctcgcc gaacggcacg atcaggaaca tcctcggcgg cgtgatcttc
     1141 cgcgagccga tcgtgatctc gaacgtgccg cgcctcgtgc ccggctggac caagccgatc
     1201 atcatcgggc gtcacgcctt cggcgaccag taccgcgcca ccgacatggt ggtcgagggc
     1261 gaaggcaccc tgaccatgac cttcaccccg aaggacgagg gcgagccgat cgagatgcat
     1321 gtccatgact tccccggtgg tggcgtcgcg atggcgatgt acaaccacga cgattcgatc
     1381 cgtgactttg cccgggcttc cttccgttac ggcctcgagc gcggctaccc ggtctacatg
     1441 tcgaccaaga acacgatcat gaagcgctac gacggccggt tcatgatcct cttccaggaa
     1501 gtcttcgatg acgagttcaa ggacgagttc gaccaggccg ggctcaccta cgagcaccgc
     1561 ctgatcgacg acatggtcgc ctcggccctg aaatgggagg gcggctacgt atgggcctgc
     1621 aagaactacg acggcgacgt ccagtccgac accgtggccc agggcttcgg ctcactcggc
     1681 ctgatgccct cggtcctgat gaccccggac ggaaagacgg tcgaggcgga agccgcccac
     1741 ggcaccgtga cgcgccacta ccgcgagcac cagaagggca acccgacctc gaccaacccg
     1801 atcgcctcga tcttcgcctg gacccggggg atcgccgccc ggggccgcat ggatgagacc
     1861 cccgaggtca gccagttcgg cgagaccctc gagaaggtct gcatcgagac ggtcgagtcc
     1921 ggcaagatga caaaggacct cgccctgctg gtcggtcccg accaggagtg gcagaccacc
     1981 caggaattcc tggcctcaat cgacgagaac ctccagaagg cgatggccta gggcccgcca
     2041 ccagaaacca aggagaagaa cgtgagttca accgccaagg taaccgtgac cggcgctgcc
     2101 ggccagatcg gttacgccct gcttttccgg atcgccagtg gacagatgct cggtcccgac
     2161 accaaggtcc acctgagcct gctcgagatc ccggccgccg tgaaggccgc cgaaggcacc
     2221 gccatggagc tcgacgactg cgccttcccg ctgctcgaga agatcagcat ctcggacgac
     2281 ccgaaggaag cgttcgacgg cgtcaacgtc ggactgctgg tcggcgcccg tccgcgtgga
     2341 ccgggaatgg aacgggccga tctgctcgag gcaaacggtg gcatcttcaa gccgcagggt
     2401 gaggcgatca acgccggcgc cgccgatgac gtgaagatcc tcgtggtcgg caacccggcc
     2461 aacaccaacg ccctgatcgc tgcctcggcc gctccggacg tgccgaagga gcgcttcacc
     2521 gcgatgatgc gcctcgacca caaccgggcg atcgcccagc tcgccaacaa gctcggccgc
     2581 tccgtttctg acgtcaagcg gatgaccgtg tggggcaacc attcggccac ccagtacccg
     2641 gacatcggca acgccctggt tgatggcgct ccggcccccg cccaggtcga tgatgaagtc
     2701 tgggtccgcg aggagttcat tccgcgggta gccaagcgtg gcgccgaagt aatcgacgcc
     2761 cgcggagcct caag
//
LOCUS       DAOQMF010000189         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21424_length_2767_cov_3.567109, whole genome shotgun sequence.
ACCESSION   DAOQMF010000189 DAOQMF010000000
VERSION     DAOQMF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21424_length_2767_cov_3.567109"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLV77_04135"
     CDS             <1..198
                     /locus_tag="PLV77_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15066.1"
                     /translation="VKGMNYTGVTKPVDAVKVTAGGASAITAYNGKATLKVKPKQSGK
                     LTVKVAKDGFRPASAKLNVRK"
     gene            complement(212..847)
                     /locus_tag="PLV77_04140"
     CDS             complement(212..847)
                     /locus_tag="PLV77_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNC15067.1"
                     /translation="MPEEIRDPNRLVTTLEPAELARIEEEVQEIRACVPAWVWDGRSL
                     PVPIDLIAREIYNLRVRVVSEEAMREAIGEDPDGHYGLSGLLICADGEIWVSKGELDY
                     DWGSRRHRFTVGHELGHFVMHQSREPRIFCREIENGESETGEVDQNDPVPRPVPEAEA
                     NTFSAAMLMPIDQVRKRLSSNPADPVKDLQDYFGVNRKPALRRVETARILG"
     gene            complement(870..1538)
                     /locus_tag="PLV77_04145"
     CDS             complement(870..1538)
                     /locus_tag="PLV77_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15068.1"
                     /translation="MNDFEETPDPVIHAFDEYVAEFRKGHGDPSPFLERFEGSERKSL
                     ETLIESFVATGPESSPDPGDPRFNAVFEKVLDEVSAPAGGLSEHLALLRQKAGKTQAA
                     VVAALAKTFEATPQEQEKIDEYYHRLEWGSLPASGLDGKLYSALAAILGVEPESLKEA
                     AGSLGSRYGASSGPVFARSLDQVDLAEDASIVPGVIGQAVPDSESRSEPSRPDRIDEL
                     FTGG"
     gene            complement(1535..2158)
                     /locus_tag="PLV77_04150"
     CDS             complement(1535..2158)
                     /locus_tag="PLV77_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC15069.1"
                     /translation="MNFKPISEHELARLKDDELISHAVAARDARKQDAFEETLKLFVG
                     RRFGLVEYWVSRKSNANDRDEIVSRAFVSIIKGIGKFRGRSTGQMVEWMRTVTGRRIA
                     DFYRDKEKTPRMVPLGEQPKEDEDWGPTNLGNPDDTGGVEVEMLIEQTLETRNEVQQE
                     VIRLRMKGYSSKEVAEMSSDQKMSFTNVDKIFSRFRQDIEKAMQDDA"
     gene            2431..>2767
                     /locus_tag="PLV77_04155"
     CDS             2431..>2767
                     /locus_tag="PLV77_04155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC15070.1"
                     /translation="MVGGGLTGLWAAVRAKERNPAREVVLIEREQLATGASGRNGGFM
                     SSSLVHGLGNGLARFPDEVPLLEKLGLENLAGIEKVIEEQGIDCDLRKPGAIEVAVTD
                     AQVADLQGGV"
BASE COUNT          495 a          880 c          843 g          549 t
ORIGIN      
        1 gtcaagggca tgaactacac cggagtgacg aagccggtcg acgcggtcaa ggtcacagcc
       61 ggcggcgcct ccgccatcac cgcctataac ggcaaggcaa ccctgaaggt caagccgaag
      121 cagtccggca agctcaccgt caaggtggcg aaagacggat tccggccggc ctccgcaaag
      181 ctgaacgtcc ggaagtagcc gcccggttcg ttcagccgag aatccgcgcc gtttcgaccc
      241 gtcgaagcgc cggcttccgg ttgacgccga aatagtcctg gagatccttg accgggtcgg
      301 ccgggttgga ggaaagtctc ttccgaacct ggtcgatcgg catcaacatc gccgccgaga
      361 aagtgttcgc ctcggcctcc ggcaccggcc tcgggaccgg gtcgttctgg tcgacctcac
      421 cggtttcact ctcgccgttt tcgatctcgc gacaaaagat cctgggctcc cggctctggt
      481 gcatcacgaa gtggccgagt tcgtggccaa ccgtgaatcg gtgccgtcgc gaaccccagt
      541 cgtaatcgag ttcgcccttg ctgacccaga tttcaccgtc agcgcaaatc aacaggccgg
      601 atagcccgta gtgaccgtcg ggatcctcac cgatcgcttc gcgcatcgct tcctcactga
      661 ccacgcgcac ccggaggttg tagatctcgc gggcgatcag gtcgattggc accggcaggc
      721 tgcggccatc ccagacccat gcgggaacgc aggccctgat ttcctgaacc tcttcctcta
      781 tccgggcaag ctcggcaggc tcgagcgtgg ttacgagcct gttcgggtct ctgatctcct
      841 ccggcacgca ggcgattgta gaaggcgggt cagccgccag tgaaaagctc gtcgattcga
      901 tctggtctgg acggttccga ccgggattca gaatccggca cggcctgacc gattacgccg
      961 ggaacgatcg aagcatcctc ggccagatcg acctgatcca gggaacgggc aaaaaccggt
     1021 cctgatgaag cgccatacct ggaaccaagc gaaccggccg cctccttcaa tgactccggt
     1081 tcaacgccga gaatcgcggc cagtgccgaa tagagttttc cgtccaagcc gctggccggc
     1141 agcgaacccc attcgagccg gtggtagtac tcgtcgatct tttcctgctc ttgtggggtg
     1201 gcctcaaagg tttttgccaa agcggcaacc accgcagcct gggtcttgcc tgccttctgt
     1261 ctcagcaggg cgagatgttc ggaaagaccg cctgccggag ccgaaacctc gtcgaggacc
     1321 ttctcgaaga ccgcgttgaa tcgggggtca cccgggtccg ggcttgactc gggcccggtt
     1381 gccacgaagg actcgatcag agtttcgagg gacttccgtt cgctcccttc gaaacgctcg
     1441 aggaaaggcg acggatcgcc gtggcccttg cggaactcgg ccacgtactc gtcgaatgcg
     1501 tggatgaccg ggtccggggt ttcctcgaaa tcgttcatgc gtcgtcctgc attgcctttt
     1561 cgatgtcctg ccgaaaacgg gagaagatct tgtcgacgtt ggtgaaagac attttctggt
     1621 cgctgctcat ttcggcaacc tctttcgagg aatacccttt catccgcaat cggatcactt
     1681 cctgctggac ctcgttccgg gtttcaagag tctgctcaat cagcatttcg acctcgacgc
     1741 cgccggtgtc atcggggttg ccgaggttcg tcggccccca gtcctcatct tccttcggct
     1801 gttcgcccag cgggaccatc ctgggggtct tttccttgtc cctgtagaag tcggcgatcc
     1861 gccgtccggt gacggttcgc atccactcga ccatctggcc ggtactccgg ccccgaaact
     1921 tgccaatgcc cttgatgatg ctgacgaacg cccggctgac gatctcgtcc cggtcgttgg
     1981 cattggactt cctgctgacc cagtactcga caagcccgaa gcggcgcccg acaaagagtt
     2041 tgagggtctc ctcgaaagcg tcctgtttgc gcgcatcccg ggcagcaaca gcgtgggaga
     2101 tgagttcgtc atctttcagt ctcgccagtt catgttcact gatcggcttg aagttcaagc
     2161 ttgtcccgga ggtcgttgcc ttcgcattcc ttctgacatg gccctgaaat ccgctcgcgg
     2221 acgcccggaa ccaagtgcca agataccggc tgggtcggct acagtcagcc catctcccgc
     2281 ggcgcacaga acgcagtctg gcccgaatcg gaaccaggtg aatccgatcg tcgctccttt
     2341 gccgaagccg agcggtcgcc gttctggctt tccgggccgc gacccgaacc gcaccaccct
     2401 ctcgaaggtt cctccgaggc ggatctggtc gtggtcgggg gcgggctgac cgggctctgg
     2461 gcggcggtcc gggcgaagga acgcaacccg gcccgggagg tcgtgctgat cgagcgtgaa
     2521 cagcttgcga ccggggcgag cggacgcaac ggcggcttca tgtcttcctc gctggtccat
     2581 ggcctcggca atggcctcgc ccgcttcccg gatgaggtgc cgctgctcga gaagctgggc
     2641 ctggagaatc tggccgggat cgagaaggta atcgaggagc aggggatcga ctgtgacctg
     2701 aggaagcccg gggcgatcga ggtcgcggtg accgacgccc aggtggccga ccttcaaggc
     2761 ggggtcg
//
LOCUS       DAOQMF010000190         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21491_length_2761_cov_3.901700, whole genome shotgun sequence.
ACCESSION   DAOQMF010000190 DAOQMF010000000
VERSION     DAOQMF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21491_length_2761_cov_3.901700"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="PLV77_04160"
     CDS             complement(<1..1908)
                     /locus_tag="PLV77_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC15071.1"
                     /translation="MLRKSAYLALLLLSFLGLAVPVANAAPKDYSAIARNIIPSGQKG
                     TPPLDTTQAVMYDALTPLFNNVTDSDIKEYFKSEKLGTSTDGPYTTETVPKAGVTIQR
                     DKYGVPHVTATTYDGGIWAAGWIAAEDRGLLLQQARYNARVAAIDAPGLSAIGLVAGL
                     QNFQPSQRTEDEIAKQTDALKAAGSEGKAVLKDIDTYLTGINDYLDANSPATPDWTRN
                     DVYAVNALKDQFLGEGGGDEARRSQFLGGLIKRLGAKKGWKVFNDLRQHTNNESPTSI
                     DGRFAYSPIPSNKSGSVIIDPKSFKPVDVTPNTPEELSDRSSSNPLEPRHQASNTLMI
                     TKGRSATGRPLMVGGPQIGYFAPGLTYEIDMNAPGLKWRGATSAPFPGYLLIGRGQDF
                     ATTLTSASGDVIDQFAETLCGGSDTKYLYKGKCRTMGTFDAGTLNGDPVSFRTTVHGP
                     VVGYATVKGKKVAISSKRSSYGKDVLDLMFNRRLSNGSVKGPQSFFEAASKTPQTFNS
                     FYIDNKNVALYTSGKLPIRDKRVDPGLLTKGTGQYEWKGFLSKKGHPHGMNPKSGMMV
                     NWNNSVARGFGAADDEWGRNGSVQRVGLLTRMLNRNKSKNGKLNMADVVSAMNAGATQ
                     DVRAIFTVPLLA"
     gene            complement(2073..2162)
                     /locus_tag="PLV77_04165"
     tRNA            complement(2073..2162)
                     /locus_tag="PLV77_04165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2126..2128),aa:Ser,seq:gct)
     gene            2231..>2761
                     /locus_tag="PLV77_04170"
     CDS             2231..>2761
                     /locus_tag="PLV77_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC15072.1"
                     /translation="MALKEERFDGGSTGIAWRRLGEGPPLLLINGYAATKDDWDPTFL
                     AGLAEKSQVICPDNRGVGDSARSVQEITIASMATDLVSLLDDLKVGEVDVAGWSMGGF
                     IAQKLVTWIPDRIKSLTLLSTDSGGTRAHLRSPEAEAALTDKSGTPEEQADRLIDLLF
                     PAGFAAQVKATAGEIVA"
BASE COUNT          448 a          838 c          950 g          525 t
ORIGIN      
        1 ggccagcagc ggcacggtga agatggcgcg cacgtcctgg gtcgcaccgg cgttcatcgc
       61 cgaaacgacg tcagccatgt tcagcttgcc gttcttgctc ttgttccggt tcagcatccg
      121 ggtcagcagg ccaacgcgct ggaccgaacc gttgcggccc cactcgtcat cggcggcgcc
      181 gaagccacgg gcgacgctgt tgttccagtt gaccatcatt cccgacttcg gattcatgcc
      241 gtgcgggtgg cccttcttgg agaggaagcc cttccactcg tactggccgg tgcccttggt
      301 caagaggccc gggtcaaccc gcttgtcgcg gatcggcagc ttgccgctgg tgtagagcgc
      361 gacgttcttg ttgtcgatgt agaacgagtt gaaggtctgc ggggtcttcg aggcagcctc
      421 gaagaatgac tgcggaccct tgaccgaacc gttcgacagg cgacggttga acatcaggtc
      481 gagcacgtcc ttgccgtagc tggagcgctt cgaggagatc gcgaccttct tgcccttgac
      541 cgtggcgtag ccgacgaccg gaccgtggac ggttgtcctg aaggaaaccg ggtccccgtt
      601 cagggtgccg gcgtcgaaag tgcccatggt gcggcacttg cccttgtaca ggtacttggt
      661 atcgctgccg ccacagagcg tctcggcgaa ctggtcgatc acgtcgccgc tggccgaggt
      721 cagcgtcgtc gcgaagtcct ggccgcggcc gatcagcagg tagccgggga acggggccga
      781 ggtggcaccg cgccacttca gaccgggcgc gttcatgtcg atctcatagg tgagaccggg
      841 ggcgaagtag ccgatctgcg gtccgccgac catcagagga cggccggtgg ccgaacggcc
      901 cttggtgatc atcagcgtgt tcgaggcctg gtgacggggc tcgagcgggt tcgaactgct
      961 ccggtcgctg agctcctcgg gagtgttcgg ggtcacgtcg accggcttga agctcttcgg
     1021 gtcgatgatg acgctgccgg acttgttcga ggggatcggc gagtaggcga aacggccgtc
     1081 gatcgaggtc gggctctcgt tgttggtgtg ctggcgcagg tcgttgaaga ccttccagcc
     1141 cttcttcgca ccgaggcgct tgatcagacc gccgaggaac tgcgaacggc gggcttcgtc
     1201 gccgccgccc tcaccgagga actggtcctt cagcgcgttg accgcgtaga cgtcgttgcg
     1261 ggtccagtcc ggggtggccg ggctgttcgc atcgaggtaa tcgttgatgc cggtcaggta
     1321 ggtgtcgatg tccttgagga ctgccttgcc ttcggagccg gcggccttga gggcgtcggt
     1381 ctgcttggcg atctcgtctt cggtccgctg gctgggctgg aagttctgca gcccggcaac
     1441 caggccgatc gcgctgaggc ccggcgcgtc gatggcggcg acccgggcgt tgtaacgggc
     1501 ctgctgaagc agcaggccgc ggtcttccgc ggcgatccag ccggccgccc agatgccacc
     1561 gtcataggtg gtggcggtca cgtgcggcac gccgtacttg tcgcgctgaa tcgtgacgcc
     1621 ggccttcggc acggtctccg tggtgtaggg accatcggtc gaggtgccaa gcttctccga
     1681 cttgaagtac tcctttatgt cactgtctgt gacgttgttg aagagcgggg tcagcgcgtc
     1741 gtacatcacg gcctgggtgg tgtccagggg cggagtgccc ttctggccgg aggggatgat
     1801 gttgcgcgcg atcgcggagt aatccttggg agcggcgttc gcaacgggaa cggcgagacc
     1861 gaggaaactc aagagcagta acgccagata ggcgctcttt cgaagcatga agtttctcct
     1921 tggtaccggt ccggagggcg gcattcggcg tcgccactca gaccgggttg tcatgagggt
     1981 tgattggagg ctactccgat cagaacaccc ggattcgcct atgggtgcgg tggagggtcg
     2041 gccggggccg gttcagcgac gccggatctg agcggagagg gagggattcg aaccctcgat
     2101 acggctataa accgcatact cccttagcag gggagcgcct tcagccactc ggccacctct
     2161 ccgggccggg gaattgtatg agttgctgaa agatgcagtc ggccaacccc ttctgaccta
     2221 gacttcgccc atggcgctga aggaggagcg gttcgacgga ggctccacgg ggatcgcctg
     2281 gcggcggctg ggtgaaggac ccccgctcct gctgatcaac ggctatgcgg cgaccaagga
     2341 tgactgggac cccacctttc tggccgggct cgccgaaaaa tcccaggtga tctgccccga
     2401 caaccgcggg gtcggagact cggcccgcag cgttcaggag atcacgatcg cctcgatggc
     2461 gaccgacctg gtttcactcc tcgatgacct gaaagtcggg gaggttgacg tcgccggctg
     2521 gtcgatgggc ggattcatcg cccagaaact ggtcacctgg atacccgacc ggatcaagtc
     2581 cctgaccctg ctctccaccg acagcggtgg cacccgggcc caccttcgca gccccgaggc
     2641 cgaagccgcc ctcaccgaca agtccggcac cccggaggaa caggccgacc ggttgatcga
     2701 cctgctcttc ccggcagggt tcgcggccca ggtgaaagcc acggcgggtg agatcgtcgc
     2761 c
//
LOCUS       DAOQMF010000191         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21706_length_2746_cov_3.408770, whole genome shotgun sequence.
ACCESSION   DAOQMF010000191 DAOQMF010000000
VERSION     DAOQMF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21706_length_2746_cov_3.408770"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLV77_04175"
     CDS             complement(<1..826)
                     /locus_tag="PLV77_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC15073.1"
                     /translation="MPLLEPDVMKISPEASEPALDRAPDWVAGGTPEPLRSRLTALLG
                     EEKLLGRATDLIRYASDASPYRMVPKAVVMAHSAGDVAKVLEFGRREAIPVTFRSGGT
                     SLNGQSQTEGILVDVRKHFTGVEVIDDGRQVRVGPGTVLGHANKVLAPYGRKLGPDPA
                     STDIATVGGVVNNNSGGMRCGVTYDSYQTVTGMTFVLPSGTMINTEDENAEADFAAKE
                     PELVEGLMKIREEILADQVLVDRIRHKFEIKNTTGYRLCAFLDGETPVEIFRRLIIG"
     gene            complement(851..2446)
                     /gene="mdlC"
                     /locus_tag="PLV77_04180"
     CDS             complement(851..2446)
                     /gene="mdlC"
                     /locus_tag="PLV77_04180"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HNC15074.1"
                     /translation="MPTVREATFDLFRKLGMTTIFGNPGSTELPMLADYPDDFEYVLG
                     LQEAAVVGMADGHAQVTGKPTLVNLHTAPGLGNAVGALFNAQANHSPVVVTVGQQVRA
                     QVTMQANLTNRDAIRVGDPFVKYSYEPARAEDVPLAIAHAIHLASLPPMGPVVVSIPM
                     DDWDVEMNAAEAQHVIDRAVVGRSGADPTVVADLAARLKQAENPVFVAGPDIDAAGGW
                     DEAIALAEGQNLPVWASPATGGGRLGFPESHPHWRGVLPPGIAAIAEVLKEHDLILVV
                     GSSVFPYYPYMPGDFLGENSKLVQITSDPDEAARAPMGEAIVADVKLTLAALNGELGG
                     TERPAIEPWPGPTRMEETDPLNPSVVMNTLKDVMPEDAVIVLEAPTSTMALRNQLRIS
                     KPNSYFFGAGGGLGFGLSSAVGIGYAEDRPVICVLGEGSVQYAVTAFWTAAAYDIPVT
                     FLILRNEEYGILKWFAEVEQVSDAPGLDLPALETAKIAEGYGIESTTAKSAEEVAAAL
                     KEAIADSRPRLVEVPVEPGMSLF"
     gene            2530..>2746
                     /locus_tag="PLV77_04185"
     CDS             2530..>2746
                     /locus_tag="PLV77_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15075.1"
                     /translation="MRRTLVISLILPALALPFTGCSDGRKVATPPACLSASATWLTAL
                     SSAPDQVLLEGVTPLSDCLTKGQSAAQH"
BASE COUNT          423 a          912 c          929 g          482 t
ORIGIN      
        1 aaccgatgat caggcggcgg aagatctcta ccggggtctc gccatcgagg aaggcacaga
       61 gccggtaccc ggtggtgttc ttgatttcga acttgtgacg gatccggtca accagcacct
      121 ggtcggcgag gatctcctcg cggatcttca tcaggccttc gaccagttcc ggttccttcg
      181 cggcgaaatc cgcttccgcg ttttcgtctt cggtgttgat catcgtgccc gagggcagga
      241 cgaaggtcat cccggtgacc gtctggtacg agtcgtaggt caccccgcag cgcatgccgc
      301 cggagttgtt gttgaccacg ccgccgaccg tggcgatgtc cgtcgaagcc ggatccgggc
      361 cgagcttgcg accgtagggg gcgagcacct tgttggcgtg gccgagcacg gttcccggtc
      421 cgacccggac ctgccggccg tcgtcgatca cctcgacccc ggtgaagtgc ttgcgcacgt
      481 caaccaggat gccctcggtc tggctctggc cgttgaggct ggtgccgccg gagcggaacg
      541 tgaccgggat cgcttcgcgc cggccgaact cgagcacctt ggccacgtcc ccggcactgt
      601 gggccatgac caccgccttc ggcaccatcc ggtaggggct ggcgtccgag gcgtagcgga
      661 tcaggtcggt cgcccggccg agcagcttct cctcgccgag cagcgcggtg agccgggagc
      721 ggagcggttc gggcgtgccg cccgcaaccc agtcgggagc ccggtcgagt gccggctcgc
      781 tcgcctccgg gctgatcttc atgacatccg gttcgagcag tggcatctca gactccctga
      841 cagttggttt ctagaacagc gacattcccg gctcgaccgg cacttcgacc agccgcggcc
      901 tggaatccgc gattgcttcc ttcagcgccg cggctacttc ctcggcactc ttcgccgtcg
      961 tgctctcgat tccgtacccc tcggcgatct tcgccgtctc cagggccggc aggtcgagcc
     1021 cgggggcatc ggaaacctgc tcgacctcgg cgaaccactt gaggatcccg tactcctcgt
     1081 tgcgaaggat caggaaggtg accgggatgt cataggcggc ggcggtccag aaggcagtca
     1141 ccgcgtactg cacagaacct tcaccgagta cgcagatcac cgggcgatcc tcggcgtaac
     1201 cgatgccgac cgcggaggaa aggccgaagc cgagtccgcc gccggccccg aagaagtaac
     1261 tgttcggctt cgagatccgg agctggttgc ggagcgccat ggtcgaggtc ggagcttcga
     1321 gcacgatcac cgcgtcttcc ggcatcacgt ccttcagcgt gttcatcacg accgacgggt
     1381 tgagcggatc cgtctcttcc atccgggtcg gccccggcca cggttcgatt gccggccgct
     1441 cggtgccgcc gagctcgccg ttcagggcgg ccagggtcag tttcacgtcg gcgacgatcg
     1501 cctcgcccat cggggcgcgg gccgcctcgt ccgggtcgga ggtgatctgg accagcttcg
     1561 agttctcgcc gaggaagtcg ccgggcatgt acgggtagta gggaaagacg gacgagccga
     1621 ccaccaggat caggtcatgt tccttcagca cctcggcgat cgcggcaatg ccgggcggca
     1681 gcaccccgcg ccaatgcggg tgactttccg ggaagccaag ccggccgcca ccggttgcgg
     1741 gggaggccca gaccggcagg ttctggcctt cggccagggc gatcgcttcg tcccagccgc
     1801 cggccgcgtc aatgtcgggt ccggccacga agaccgggtt ctctgcctgc ttgaggcggg
     1861 cggcgaggtc ggcgaccacg gtcgggtcgg cgcccgatcg tccgaccact gcccggtcga
     1921 tcacatgctg tgcctcggcg gcgttcatct cgacatccca gtcgtccatc ggaatcgaaa
     1981 cgacgaccgg tcccatcggc ggaagcgacg cgaggtgaat cgcgtgggca atcgccagcg
     2041 gcacgtcttc ggcgcgggcc ggttcgtagg agtacttgac gaaggggtcc ccgacgcgga
     2101 tcgcgtcgcg gttggtcagg ttcgcctgca tcgtgacctg ggcgcgaacc tgctggccga
     2161 ccgtgaccac caccggcgag tggtttgcct gcgcgttgaa gagcgcgccg accgcgttgc
     2221 cgagacccgg ggcggtgtgg aggttgacca gggtcggctt gccggtgacc tgggcgtgac
     2281 cgtcggccat gcccaccacg gccgcctcct gcaggcccag cacgtactcg aagtcatcgg
     2341 ggtaatcggc cagcatcggc agctcggtcg agcccgggtt accgaagatc gtggtcatcc
     2401 ccagcttgcg gaaaaggtcg aaggtcgctt cgcgaacggt aggcatgggc gcagcctata
     2461 tgtgaacggg tgagtacggg accggcaggc aagggtgggg cgaaggtcct ggcctgtcag
     2521 gattgtgccg tgcgcagaac tctcgtgatc agtctgatcc ttcccgccct cgccctgccc
     2581 ttcaccggct gcagcgacgg tcgcaaggtc gcgacgccgc ccgcctgtct gagcgcctcc
     2641 gcgacgtggt tgacggcact ttcttcggca ccggatcagg tcctgctcga aggggtcacg
     2701 ccgctcagcg actgcctgac caaggggcaa tccgcggccc agcatg
//
LOCUS       DAOQMF010000192         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21722_length_2745_cov_2.326394, whole genome shotgun sequence.
ACCESSION   DAOQMF010000192 DAOQMF010000000
VERSION     DAOQMF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21722_length_2745_cov_2.326394"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /gene="prfA"
                     /locus_tag="PLV77_04190"
     CDS             <1..764
                     /gene="prfA"
                     /locus_tag="PLV77_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNC15076.1"
                     /translation="PNDDKNVIVEIRAGTGGDEAALFAGDLYKMLTRYAAERGYSTEV
                     LSQSAAEQGGFKDVTFEVRGAGAYSVYKYEGGTHRVQRVPETESQGRIHTSTATVAVL
                     PEAEEVEVDIDQNDLQIDVYRSSGPGGQSVNTTDSAVRITHQPTGIVVSMQDEKSQLQ
                     NREKAMRVLRARLYERQLAEQQAAVAAERKAQVGTGERSEKIRTYNFPQGRVTDHRIK
                     LTAHDLDGIMNGNLNQFTDALAAEEKRQRLDAQAV"
     gene            767..1603
                     /gene="prmC"
                     /locus_tag="PLV77_04195"
     CDS             767..1603
                     /gene="prmC"
                     /locus_tag="PLV77_04195"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNC15077.1"
                     /translation="MAASGASATEALAAAIDAFTAAGIETPRLDAEVLLAELAGLDRA
                     ALIADRELILEPAAARAYSEAVRRRLRREPVAYILGRKGFRHIDLAVDRRVLVPRPET
                     EMLVELAREVNPETVLEVGTGSGAIALAVADELAACQVTATDVSDGALEVAIANAGRL
                     GLGERVRFILGSLPEGPAGFDLILANLPYVPDSDGLPPEVGQWEPAGALFGGPDGTRV
                     IAEVLAGLGEAGIGAPVIGLEIGQGQGELVAGMVRDAGWPETEIRLDLAGIDRVVVGR
                     GG"
     gene            1680..2306
                     /locus_tag="PLV77_04200"
     CDS             1680..2306
                     /locus_tag="PLV77_04200"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNC15078.1"
                     /translation="MKVVATSVNAGDAARQALESCISKGGVALFPADGLYGLACDPLN
                     EKAIRRIHEIKGRDDGKPSAVMYLSPLAMRELIRDLGQRVSEAAARLLPGPVTLVVPN
                     PRHRYPLACRDDNSRLGIRVIDGPLSGVRFPLFQTSANLSGEPAPSVFEGVVPEVREM
                     VDLAIDGGRLTGLPSTVVDLTDYDEHGEWEILREGGMSRGDLIAALGR"
     gene            complement(2380..>2745)
                     /locus_tag="PLV77_04205"
     CDS             complement(2380..>2745)
                     /locus_tag="PLV77_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HNC15079.1"
                     /translation="RVNAAGKKIELVRTGPKQFYCFRDLVQTNPASTRSPDSAVYPAC
                     NQNPRIRHVTLGTSVGWSDIYPSTYDRQWINVTGLRGCFAYVLHLDPGNVLFELSKKN
                     NYAQRIVRLPWRGYDRRGC"
BASE COUNT          439 a          899 c          981 g          426 t
ORIGIN      
        1 atccgaacga cgacaagaac gtgatcgtag aaatccgggc tggcaccggt ggcgacgagg
       61 ccgccctgtt tgccggtgat ctctacaaga tgctcacccg ttacgccgcc gagcgcggct
      121 actcgaccga ggtgctttcc cagtcggcgg ccgagcaggg tggcttcaag gacgtgacct
      181 tcgaggtccg gggcgccggc gcctactcgg tttacaagta cgagggcgga acccaccggg
      241 tccagcgcgt gccggagacc gaatcccagg gccggatcca cacctcgacc gcgaccgtgg
      301 ccgtgctccc ggaggccgag gaggtcgagg tggacatcga ccagaacgat cttcagatcg
      361 acgtctaccg ctcgtcgggg ccaggcgggc agtcggtcaa caccaccgat tcagccgtcc
      421 ggatcaccca tcagccgacc ggcatcgtcg tgtcgatgca ggacgagaag tcccagcttc
      481 aaaacaggga gaaggcgatg cgggtgttgc gggcccgtct ttacgagcgg caactggccg
      541 aacagcaggc ggcggtggcc gccgaacgca aggcccaggt cggaaccggg gagcgctccg
      601 agaagatccg cacctacaac ttcccccagg gccgggtgac cgaccaccgg atcaagctga
      661 ccgcccacga ccttgacgga atcatgaacg ggaacctcaa ccagttcacc gacgcgctgg
      721 ccgccgagga aaagcgccag cgcctcgacg cccaggcggt ctgatcgtgg ccgcctccgg
      781 ggcgagcgcc accgaggccc tcgccgcggc gatcgacgcc ttcacggcgg ccgggatcga
      841 gacgccccgg ctggacgcgg aggtcctgct ggcggagctg gccggactcg accgcgccgc
      901 cctgatcgcc gaccgcgaac tgatcctgga accggcggcc gcccgggcct attcggaagc
      961 ggtccgtcgt cggctgaggc gcgagccggt cgcctacatc ctcggccgca agggctttcg
     1021 ccacatcgac ctcgccgtcg atcgccgggt tctcgttccc cggccggaaa ccgagatgct
     1081 ggtcgaactg gcccgggagg tgaacccgga gacggtgctt gaggtcggga ccggctccgg
     1141 ggcgatcgcc ctggccgtcg ccgacgaact tgctgcctgc caggtgaccg cgaccgatgt
     1201 ctccgacggg gcactcgagg tggcgatcgc aaacgcggga cggctcggcc tgggggagcg
     1261 ggtccgcttc atcctcgggt ctttgccgga aggcccggcc gggtttgacc tgatcctcgc
     1321 caacctcccg tatgtgcccg actcggatgg cctgccgccc gaggtcgggc aatgggagcc
     1381 cgcgggtgcg ctgttcggcg ggccggacgg aacccgggtg atcgcggagg tgctcgcggg
     1441 gctgggggaa gccggaatcg gggccccggt gattggcctc gagatcggcc aggggcaggg
     1501 ggagctggtg gccgggatgg tccgcgatgc cggctggccg gaaaccgaaa tccgcctcga
     1561 tctggccggt atcgaccggg tcgtggtcgg gcgtggcggc tgagcctgtt cggccgaccg
     1621 ggctgactgc cgggcccgct ctttcgccgc gggcgatccc ggcctgacat gatcgggttg
     1681 tgaaggtcgt agcgacaagc gtcaacgccg gcgacgccgc ccgccaggca ctcgagtcct
     1741 gcatcagcaa gggcggagtc gccctgtttc cggccgacgg cctctacggg ctggcctgtg
     1801 accccctgaa cgagaaggcg attcgccgca tccacgagat caagggccgc gatgacggca
     1861 agccgtcggc ggtcatgtat ctgtcaccgc tggcaatgcg cgaactgatc cgcgacctcg
     1921 gtcagcgggt ctccgaggcg gccgcgaggc tgctgcccgg gccggtcacc ctggtcgtcc
     1981 cgaatccccg gcaccgctat ccgctggcct gccgcgacga caattcccgg ctcggcatca
     2041 gggtgattga cgggcccctg tccggggtgc gctttccgct cttccagacc agcgccaacc
     2101 tttccggcga gccggctccc tcggtcttcg agggcgttgt gcccgaggtt cgcgaaatgg
     2161 ttgacctggc gatcgacggc ggccggctga ccggcctgcc ctcgaccgtg gtcgacctga
     2221 ccgactatga cgagcacggc gaatgggaga tcctccgcga aggcgggatg agccggggcg
     2281 acctgatcgc cgccctcggt cgttagggat tcatcgaatg cccggtttgt gccggtatgc
     2341 ggcgcaaagt ggggtctcgc ggtggtcgca gcggcagggt cagcagccgc ggcgatcgta
     2401 accgcgccag gggaggcgga cgattcgctg ggcgtagttg ttcttcttgc tcaattcgaa
     2461 gagcacgttg cccggatcga ggtgaagcac gtaggcgaag cagccgcgca ggccggtgac
     2521 gttgatccac tgccggtcgt aggtcgaggg gtagatgtcg gaccagccga ccgaagtgcc
     2581 aagggtgacg tggcggatcc gcgggttctg gttgcaggcg ggatagaccg ctgaatcggg
     2641 agagcgggtc gaggccgggt tggtctgaac caggtcccgg aagcagtaga actgcttcgg
     2701 cccggtacgg accagttcga tcttcttgcc tgccgcgttc acccg
//
LOCUS       DAOQMF010000193         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21782_length_2741_cov_2.653760, whole genome shotgun sequence.
ACCESSION   DAOQMF010000193 DAOQMF010000000
VERSION     DAOQMF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21782_length_2741_cov_2.653760"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1074
                     /locus_tag="PLV77_04210"
     CDS             196..1074
                     /locus_tag="PLV77_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15080.1"
                     /translation="MVEGEVTTTVRVVRLLITGLVLAAVVAGIVRGLTTPDSGGGAVV
                     SGLFLTQVALAAVLILLGGFVEGLGFGLSLGTNWPYTSNIWVLLIQGDPEAAHRIVAT
                     LVGLIGLVLVTLAPTSATINGLILIVLTAALGMGTLHVLSGRLPSLVHGVHGLLAYGV
                     FVSYLMTIYQPGVNFWSYLSDTPTLCVVLFAVLLGGMTTGHRGFGEPIGAFSWPQRAP
                     QVVMIIHLFAALMAVAALGSTMPDYPVAFYLAVAQVVVGFLLFHAVNLKPRTPGVLVA
                     VHQSVVLAITVAIVAA"
     gene            complement(1109..2329)
                     /locus_tag="PLV77_04215"
     CDS             complement(1109..2329)
                     /locus_tag="PLV77_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15081.1"
                     /translation="MNQQGDPVSAEAEPKTQVDESSAAKARKRRRALPTVLIVLATVV
                     GIISVFALWAQRQLMEQDTWSNTSQQLVENGEIQSALSDFIVASIYDNVDVEQSLADR
                     LPPDLAPIAGPVASALRGPTEKIADKALQQPKIQGLFVDASDATHQKFVQLIEDKGEF
                     VSTSGGVVTLDLKSLLTSVTSQLGVGSKAADRLPASAASIEIMKSTELSQVQTGVKAL
                     KTAAWFLTALTLVLFAAAILLGRGRRRESLRSVGVSLVFIGLVVLFARNFASNQIVNS
                     LSGSAANEAAVNSAFDIITSLLRDTGQSILVYGIAVILAAWFAGPARWAVSARRAVTP
                     WLRQPQYAFGALALILIVLFWWGPLVATQRLIPSLVLIALSAAGVEVLRRQVIQEFPD
                     LKTPDVPAGPEATP"
     gene            complement(2419..>2741)
                     /locus_tag="PLV77_04220"
     CDS             complement(2419..>2741)
                     /locus_tag="PLV77_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15082.1"
                     /translation="IVASAAPAQAKAPPRGKYDCTISGNLFGVLTIKSKGRYSHRGSS
                     GTFRAKGGSVRFPDGIVAWRISFKGGSLGGMKGRWHRTSDGVDEIALKNPINGFESIY
                     CDRR"
BASE COUNT          458 a          848 c          905 g          530 t
ORIGIN      
        1 gcccccgcta tggtcttgga acccgctcac cgagcgggtt tcgcggttaa cggggggagc
       61 tcatgctgac cgtcgtcgtc tctggatggg tcgactggct gtcagaaccg ttattgaccg
      121 catccgatgc cgtcgtctca ggctaaaatc cggcagatcg accagtccac tttccccccg
      181 agctcgcaat cctccgtggt ggagggcgag gtgaccacca ctgttcgagt ggtcaggctc
      241 ctgatcaccg ggctcgtatt ggcggccgtg gtcgcgggca tcgtgcgtgg cctcaccacc
      301 ccggattcgg gcgggggggc cgtagtgagc ggcctcttcc tgacccaggt ggcgctggct
      361 gcggtcctta ttctgctcgg aggcttcgtc gagggtttgg gcttcgggct ctcgctgggc
      421 accaattggc cgtataccag caacatctgg gtgctcttga tccagggcga cccggaggcg
      481 gcccaccgga tcgtcgccac actggtcggc ctgatcgggc tcgtgctggt gaccctggcc
      541 ccgacctcgg caacgatcaa cggcctgatc ctgatcgtgt tgacggctgc gctggggatg
      601 gggacgctcc atgtgctctc cggccggctt ccctctctgg tgcatggcgt tcacggcctt
      661 cttgcctacg gagtgttcgt cagttacctg atgaccattt atcagcccgg agtcaacttc
      721 tggtcctacc tgagtgatac gcccacgctc tgtgtggtgc tcttcgctgt gctcctgggt
      781 ggcatgacca ctgggcatcg cggcttcggc gagccgatcg gagccttctc ctggccgcag
      841 cgcgcgccgc aggtggtgat gattatccac ctgtttgccg ccttgatggc ggtcgcggcc
      901 ctggggagca caatgccgga ttacccggtg gcgttctatt tggccgtcgc ccaggtagtc
      961 gtgggtttct tactctttca cgcggtgaat ctcaagccga ggacccccgg cgttctcgtt
     1021 gcagtccacc agtccgtggt actcgccata accgtggcca tcgtggccgc atagcctcga
     1081 tcgccacatc atccttctaa aggccccgct aaggggttgc ttcgggacct gcggggacat
     1141 ctggggtctt gaggtcggga aactcctgga tcacctggcg gcgcagaact tcgactccgg
     1201 cagccgagag ggcgatcagg acgagcgagg gaatcagcct ctgcgtcgcg actagcggtc
     1261 cccaccagaa gagcacgatg aggatcagcg ccagtgctcc gaacgcgtac tgcggctgac
     1321 ggagccacgg ggtcacggcg cgacgggcgg agaccgccca gcgagccggt ccggcgaacc
     1381 aggccgcgag gatgaccgcg atgccgtaga cgaggatcga ctggcccgtg tccctgagca
     1441 gcgaagtgat gatgtcgaaa gccgaattga cggcagcttc gtttgccgcc gacccgctca
     1501 gggaattgac gatctgattc gaagcgaagt tgcgggcgaa caggacgacc agtccgatga
     1561 agacgaggga gacgccgacg ctccggaggc tctcgcgtcg acggccgcgg ccgagaagga
     1621 tcgccgcagc gaagagcacg agagtcagcg ccgtgaggaa ccaggcggcc gtcttcaggg
     1681 ccttgacgcc ggtctgcacc tgggagagct cggtggactt catgatctcg atggaagcgg
     1741 cgctcgcagg gagcctgtcg gccgctttgg aaccgacgcc gagctgagag gtcactgaag
     1801 tgaggaggct cttcaggtca agggtgacga ctccgcctga ggtggagacg aactcgccct
     1861 tgtcttcgat cagctggacg aacttctggt gggtcgcatc gctggcgtcg acaaaaaggc
     1921 cctggatctt tggctgctga agggctttgt cggcgatctt ctcggttggg ccgcgcagag
     1981 cggaggcaac cggcccggcg atcggagcca gatccggagg cagacggtcg gccaggctct
     2041 gctcgacgtc gacattgtcg tagatgctgg cgacgatgaa gtcggaaagc gccgactgga
     2101 tctcaccgtt ctcgaccagt tgctgactgg tgttcgacca ggtgtcctgc tccatcagct
     2161 gacgctgggc ccagagggca aacaccgaaa tgatcccgac cacggtcgcg agaacgatca
     2221 ggacggtggg cagtgcccga cgccgtttcc gggccttcgc cgcggaactt tcatctacct
     2281 gggtcttggg ttcggcctcg gcgctcacgg gatctccttg ctggttcacg cgaagaaact
     2341 agacgactac acggaacttc ggacgataag cccctcggag ccccgcctga tcaccccgat
     2401 caggtgaagc ggggaaggct accggcggtc gcagtagatg ctctcgaatc cgtttatggg
     2461 gttcttcagg gcgatctcgt caacgccgtc cgaggtccgg tgccagcgac ccttcatgcc
     2521 cccgagagat ccgcccttga agctgatcct ccaagccacg atgccgtcgg gaaaacggac
     2581 cgagccgccc ttcgcccgaa aggtaccgct gctgccgcgg tggctgtacc tgcccttgct
     2641 cttgatggtc agcaccccga aaaggtttcc gctgatcgtg cagtcgtact tgccgcgagg
     2701 gggcgccttc gcctgggccg gggcggccga ggcgacgatc g
//
LOCUS       DAOQMF010000194         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21885_length_2734_cov_3.954834, whole genome shotgun sequence.
ACCESSION   DAOQMF010000194 DAOQMF010000000
VERSION     DAOQMF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21885_length_2734_cov_3.954834"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLV77_04225"
     CDS             complement(<1..255)
                     /locus_tag="PLV77_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15083.1"
                     /translation="MKRIKAGWQLTKKSWRVLSDSPGLVRFPLIGGLIALLITIVLIG
                     PGLYLLEDGNSAPGAILAAVGIYLAAFATFYFAVGLAHNGG"
     gene            complement(252..1679)
                     /locus_tag="PLV77_04230"
     CDS             complement(252..1679)
                     /locus_tag="PLV77_04230"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNC15084.1"
                     /translation="MADDSKIPKGRLRRSAKLGTAIGSQGAKYAGTKASGLFRGEDSS
                     QEKLDERHAETAAKMVATLGQMKGAAMKIGQFASFIDIDFIPEEYREIYQEQLAKLRS
                     EAPSMPWEEVVKVLKEEYDGEPIEQLFDSIEHEAFAAASIGQVHRGRLIDGREVAVKI
                     QYPGIAEALESDLKNAGMLVRMARALAPGLDAREVTRELRERVLEELDYEYEAQNQRS
                     FARAYRGHPFIYVPDVITRLSRRRVLVTEFVEGIGFEEILELGDEERSRFGEILFRGS
                     FGSIYHLQHFNADPHPGNYLLMKDGRVAFLDFGMTKKLDAEQIELEQLAVDAAVRNDP
                     EGLRDALHNLGFVKKPSKLDAEHLMNHVMSIGGWYMEDREIEIDGDRVMKVIESTHDP
                     RSEYFDLMRRESIPADELMGRRMEIGVLAVLGRLRAKRNWHRIMREWVYADPPATELG
                     REEWEYFEERGIFQVPGIKKGKLES"
     gene            1718..2407
                     /locus_tag="PLV77_04235"
     CDS             1718..2407
                     /locus_tag="PLV77_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNC15085.1"
                     /translation="MSSDRATLNATTRTDFGSRISKRLRRDGKVPGVVYSDGKEATTF
                     QVESRDARVILGEGHALFDLHIEGSDAVPVVVKEQQHHPVRGDLQHLDLQQVDLNQAI
                     QAEVAVELTGDDVSPGVKQGGVLEHVTREVTVEALPTDIPDSITLDVSEMEINDTLTL
                     EQLVAPEGVTLIADDPAEVTLVTLSPPRVEEEPEVDLEAEPEVVGEGEEGGEEAAEGE
                     EGGDEGSSGEE"
     gene            2465..>2734
                     /locus_tag="PLV77_04240"
     CDS             2465..>2734
                     /locus_tag="PLV77_04240"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNC15086.1"
                     /translation="MVGLGNPGREYERSRHNAGFMVAARLAERWDMPRAKDKFGARIT
                     EGRIRPGGPRVVLMTPLTYMNESGKSAGPARGSKKIPLDRVIAVYD"
BASE COUNT          484 a          913 c          858 g          479 t
ORIGIN      
        1 gccgccgttg tgggcgagcc cgaccgcgaa gtagaaggtg gcaaaggccg caaggtagat
       61 gccaaccgcc gcgagaatcg ccccgggagc gctgtttccg tcctcgagca ggtagagccc
      121 ggggccgatc agcacgatcg tgatcaggag tgcgatcagg ccgccgatca gcgggaaccg
      181 caccaggccg ggcgagtccg aaagaacgcg ccagcttttc ttggtcaact gccaaccagc
      241 tttgatgcgc ttcatgactc caatttaccc ttcttgatcc cggggacctg aaaaatccct
      301 ctctcttcga agtactccca ttcctccctg cccagctcgg tcgccggcgg gtcggcgtac
      361 acccactcgc gcatgatccg gtgccagttg cgcttcgccc gcagccggcc gagcacggcc
      421 agcacaccga tctccatccg gcggcccatc aactcgtcgg ccgggatcga ttcccgtcgc
      481 atcaggtcga agtactcgga gcggggatcg tgggttgact cgatcacctt catcacccgg
      541 tcgccgtcga tctcgatctc gcggtcttcc atgtaccagc caccgatcga catcacgtgg
      601 ttcatcaggt gctcggcatc cagcttcgac ggcttcttga cgaagcccag gttgtggagc
      661 gcgtcgcgaa ggccttccgg atcgtttcga accgccgcgt cgaccgccag ctgttcgagc
      721 tcgatctgct ccgcgtcaag cttcttggtc atgccgaaat cgaggaaggc aacccggccg
      781 tccttcatca acaggtaatt gccggggtga gggtcggcgt tgaagtgctg caggtggtag
      841 atcgagccga agctgccgcg gaagaggatc tcgccgaaac ggctgcgctc ctcgtcaccg
      901 agttcgagga tctcctcgaa gccgatcccc tcgacgaact cggtgaccag cacccggcgc
      961 cgggaaagac gggtgatcac atcggggacg tagatgaacg gatggccacg gtaggcgcgg
     1021 gcgaaggaac gctggttctg ggcttcgtat tcgtagtcga gctcttccag caccctttcg
     1081 cggagttcgc gggtgacttc ccgggcgtcc agccccgggg cgagcgcccg ggccatccgc
     1141 accagcatcc cggcattctt gagatccgac tccagcgcct cggcgatccc cgggtactgg
     1201 atcttgacgg cgacctcgcg tccgtcgatc aggcggccgc ggtggacctg gccgatcgag
     1261 gcggcggcga aagcttcgtg ctcgatcgag tcgaagagct gctcgatcgg ctcgccgtcg
     1321 tattcctcct tcagcacctt gacgacctct tcccagggca tcgaaggcgc ttcggaacgc
     1381 agtttcgcca gctgctcctg gtagatctcg cggtactcct cggggatgaa gtcgatgtcg
     1441 atgaaggagg cgaactggcc gatcttcatc gccgcgccct tcatctgacc gagcgtggcg
     1501 accatcttcg cggcggtttc ggcatgccgt tcgtccagct tctcctggct cgaatcttca
     1561 ccccgaaaca ggcccgaggc ctttgtcccg gcgtacttgg cgccttgcga gccgatcgcc
     1621 gtgcccagct ttgccgagcg acggagcctg cccttgggga tctttgaatc gtccgccatg
     1681 cctcaagcgt agaactttcc ctatcctgtt ctgtccaatg tcttcagatc gagcaacact
     1741 caacgccaca acccgtaccg atttcggctc ccgaatctcc aagcgcctcc gccgtgacgg
     1801 caaggtcccc ggggtcgtct actcggacgg caaggaagcg accacctttc aggtcgaaag
     1861 tcgcgacgcc cgggtgatcc tgggtgaagg ccacgcgctg ttcgatcttc acatcgaagg
     1921 cagcgatgcg gttccggtcg tggtcaagga gcagcagcac cacccggtgc gcggcgacct
     1981 ccagcacctc gacctccaac aggtcgacct caaccaggcg attcaggccg aggtcgcggt
     2041 cgagctgacc ggtgacgacg tcagccccgg cgtcaagcag ggcggcgtgc tcgagcatgt
     2101 cacccgcgaa gtgaccgtcg aggcgctgcc gaccgacatc ccggactcga tcaccctcga
     2161 cgtctccgag atggagatca acgacacgct caccctcgag cagctcgttg cgcccgaagg
     2221 cgtcacgctg atcgccgatg atcccgccga agtcaccctg gtgaccctct cgccgccgcg
     2281 cgtcgaagag gagccggaag tggatctgga agccgagccg gaagtggtcg gcgaaggcga
     2341 agagggtggc gaggaagctg ccgaaggcga agagggcggc gacgaaggct cctccggcga
     2401 ggagtaggcc ctgtctttcc tgcgccgctc cgacccggaa gaagggtccg atgccccgat
     2461 cctcgtggtc gggctcggca acccgggcag ggagtacgag cgcagccgcc acaacgccgg
     2521 tttcatggtc gcggcccggc tggccgagcg ctgggacatg ccgcgggcca aggacaagtt
     2581 cggcgccagg atcaccgagg gccggatccg gcccggcggc ccgcgagtgg tgctgatgac
     2641 cccgctcacc tacatgaacg agtccgggaa gtcggccggc ccggctcgcg ggtcaaagaa
     2701 gatcccgctc gaccgggtta tcgccgtcta cgac
//
LOCUS       DAOQMF010000195         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21903_length_2733_cov_2.927184, whole genome shotgun sequence.
ACCESSION   DAOQMF010000195 DAOQMF010000000
VERSION     DAOQMF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21903_length_2733_cov_2.927184"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLV77_04245"
     CDS             <1..393
                     /locus_tag="PLV77_04245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNC15087.1"
                     /translation="GPGSKEHTKKWSAIVDRADAFVFVTPEYNATPAPALKNAIDYLY
                     HEWSYKPCSFVSYGGVWAGARAVQALKPTLTQLSVMPVPEMVPIPGVSGLLDDQGNLQ
                     GTEIHEKSAGGMLNSLEKWSGALKTLRQ"
     gene            complement(477..>2733)
                     /locus_tag="PLV77_04250"
     CDS             complement(477..>2733)
                     /locus_tag="PLV77_04250"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNC15088.1"
                     /translation="LAFGASRATAAEPAVFEVGAASSSINPDSPQYVGGYGYKQGPTQ
                     TTHDNLEARAFVVGKGKDALAFVSVDLVGWFAAYDGEQAPYGIDATREKIADALNSRG
                     YDIGRESVIVSSTHVHAAPTVVGIWGTLDGEYLKKVSDAAVTAASEAADAARPSEIWT
                     GVGDIRSFIWQNGQGTNHPDGFEYDNALPILWARDPDTGATNALYANVPNHPDQYQVD
                     DNNFEMSADWPGYARRKLDALNGGTAVLAAGTLGRQEPPGSVTAYSEVEAQGEIVANE
                     IQRTMAKSTPLTDDTIAASEQFMETPADNDDLLLGISIFTYPGACIDGIERCTIPRSK
                     APPYMNEVSGEDVIGTYVEAARIGDVAFATNPGEAFPEVNFAIRDGVTGPRQVNVIAQ
                     AGDMLGYYYQRADYTDQQFGSSDFEDYNVGPELGQANANSALAGLNEIGFTATPETVH
                     AAFESSVASKPGIQWYPDEYESTDPTFNILGSAARSQDGTAPVPTSISWDFGDTTTGT
                     TSVGERFDHTFPGPGSYEVTATVSSNGLTRSWTDTITVDPPLTADASVAKRTGQGASL
                     LGGALAGVGKIVAAHWTCQDGKKVSGLKVLCKSPKAGKASVTVVDGAGNTAKATVPVA
                     KAPPASLTIRKISVKPTKIKYGKKGTMTVAVKNPSTTNATGFKVCVKVPARAKKQIKA
                     MPACKSPGNLRAGKSASVSIRIQVTRQASPGMKVKLPVETSANGAKKKTASISILGKP
                     GGAVSSSYAAG"
BASE COUNT          398 a          868 c          941 g          526 t
ORIGIN      
        1 ggcccgggca gcaaggagca cacgaagaag tggagcgcga tcgtcgaccg ggctgacgcc
       61 ttcgtcttcg tcaccccgga atacaacgcg accccggccc cggcgctcaa gaacgcgatt
      121 gactacctct accacgagtg gtcatacaag ccctgctcct ttgtcagcta cggcggagtc
      181 tgggccgggg cccgcgcggt ccaggccctg aagccgacgc tgacccagct gtcggtgatg
      241 ccggtgccgg agatggtgcc gatccccggg gtttccgggc tgctcgatga ccaggggaat
      301 ctccagggca ccgagatcca cgaaaagagc gccggcggca tgctgaattc gctcgaaaag
      361 tggtcgggcg ccctgaagac cctgcggcag taacccgaaa aactcaagcc cgaaaaactc
      421 aaggtgccgc acgttcagcc ccgtcacggg gcagaacgtg cggcaccttt gtcagctcaa
      481 ccggcggcgt agctcgaaga caccgcgccg cccggcttgc ccaggatcga gatcgatgcg
      541 gtcttcttct tggccccgtt ggcgcttgtc tcgaccggca gtttcacctt catgcccggg
      601 ctggcctggc gggtgacctg gatccggatt gaaaccgagg ccgactttcc ggcacgcagg
      661 ttacccggcg acttgcaggc cggcatcgcc ttgatctgtt tcttcgccct cgccggaacc
      721 ttgacgcaga ccttgaaccc ggtcgcgttc gtggtgctgg ggttcttcac ggccacggtc
      781 atggtcccct ttttgccgta cttgattttg gtcggcttga cgctgatctt cctgatcgtc
      841 aggctggccg gcggggcctt ggccacgggg accgttgctt ttgccgtgtt gccggcgcca
      901 tcgaccacgg tcaccgaggc cttgccggcc ttcgggctct tgcagagcac cttgagtccg
      961 ctgaccttct tgccgtcctg gcaggtccag tgggcggcca cgatcttgcc gacaccggcg
     1021 agggcgccgc cgaggaggct ggctccctgg ccggtccgtt tggcgacgct ggcatcggcc
     1081 gtgagtggcg ggtcaaccgt gatcgtgtcg gtccagctcc gggtcaggcc gttgctgctc
     1141 accgttgccg tgacttcgta gctgcccggc cccgggaagg tgtggtcgaa gcgctcgccg
     1201 acgctggtgg tgccggtcgt ggtgtctccg aagtcccagc tgatcgaggt cgggaccggc
     1261 gcggtgccgt cctgtgacct ggcggccgag ccgaggatgt tgaaggtcgg atcggtcgac
     1321 tcgtactcgt cggggtacca ctggatgccc ggcttgctgg ccacgctgct ttcaaacgct
     1381 gcgtggacgg tctccggcgt ggcggtgaac ccgatctcat tgagcccggc gagcgcgctg
     1441 ttggcgttgg cctggcccag ctccggtccg acgttgtaat cctcgaagtc gctcgagccg
     1501 aactgctggt cggtgtagtc ggcgcgctgg tagtagtagc cgagcatgtc gccggcctgg
     1561 gcgatcacgt tgacctggcg ggggccggtc acaccgtcgc ggatcgcgaa attgacctcg
     1621 gggaaggctt caccggggtt ggtcgcgaac gcgacgtcac cgatccgggc ggcctcgaca
     1681 taggtgccga tcacatcttc tccggagacc tcattcatgt agggcggggc cttcgaacgc
     1741 gggatggtgc agcgttcgat cccgtcgatg caggccccgg gataggtgaa gatgctgatc
     1801 ccgagcagca ggtcgtcgtt gtctgcgggg gtttccatga actgctcgct ggccgcgatc
     1861 gtgtcatcgg tcagcggcgt ggacttggcc atggtccgct ggatctcgtt ggcgacgatc
     1921 tcgccctggg cttcgacctc ggaatacgcg gtgacgcttc ccgggggttc ctgacggccc
     1981 agcgtgccgg ccgccagcac cgcggtgccg ccgttcaggg catcgagctt gcgcctggcg
     2041 tagccgggcc agtcggccga catctcgaag ttgttgtcgt ccacctggta ctggtccggg
     2101 tggttcggga cgttggcgta gagggcattc gtcgcgccgg tgtcgggatc acgggcccag
     2161 aggatcggca gggcgttgtc gtactcgaaa ccgtcagggt gattggtgcc ctgaccgttt
     2221 tgccagatga acgaacggat gtcaccgacc ccggtccaga tctcggacgg cctggcggcg
     2281 tcggcggcct ccgaagcggc ggtcaccgcg gcgtcgctta ccttcttcag gtactcgccg
     2341 tcgagggtgc cccagattcc gaccacggtc ggtgccgcgt gaacgtgggt gctggagacg
     2401 atcaccgatt cgcggccgat gtcgtagccg cgcgagttga gggcgtcggc gatcttctcc
     2461 cgggtggcgt cgattccgta gggggcctgt tcaccgtcgt aggcggcgaa ccagccgacc
     2521 aggtcaaccg aaacgaaggc gagcgcatcc ttgcccttgc cgaccacgaa ggcgcgggcc
     2581 tcgaggttgt cgtgggtggt ctgggtcggg ccctgcttgt agccgtaccc accgacgtac
     2641 tgcggcgaat ccgggttgat gctcgacgaa gcggccccga cctcgaatac ggccggctcg
     2701 gctgccgtgg cacgactcgc gccgaaagcc agg
//
LOCUS       DAOQMF010000196         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_21925_length_2731_cov_3.789238, whole genome shotgun sequence.
ACCESSION   DAOQMF010000196 DAOQMF010000000
VERSION     DAOQMF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21925_length_2731_cov_3.789238"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="PLV77_04255"
     CDS             complement(<1..1425)
                     /locus_tag="PLV77_04255"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNC15089.1"
                     /translation="MDTDEQPLIIVSNRGPAQFDLGPEGERVLSRGGGGLVTALSGLV
                     KDRHALWIASAMTSEDVVVTEENDGRPIDVNLNGVDYRVLMVESDPQAYDRFYNVIAN
                     PILWFIQHYLWDLSNAPDIRREEITAWYDGYEVVNEDIARCVIEQIADREDPLVMLHD
                     YHLYTAPAKIRSARPDVLLHHFVHIPWTQPDSWRVLPTPIREAIFNGLLANDIIGFHT
                     QSYCSNFLSCCRRLMGYEVDYEENVVTHPGGRTMVRAYPLSIDSDRLERAVHSERVAE
                     LEKEILARRREHLILRVDRADLSKNVLRGFTAFDTFLTDHPEFREKVTFVAHLQPSRQ
                     DVPEYAEYLERIEALVAVVNHRHGTTDWMPIDLRIYDDFDEAVARYKHCDLLMVNAIF
                     DGMNLVAKEAPVINVRDGVLMLSENTGSHEELKEFALTVNPFDIQDQADVMYRALTMS
                     PEERRERAEGLKRIIHSRTPADWID"
     gene            complement(1491..>2731)
                     /locus_tag="PLV77_04260"
     CDS             complement(1491..>2731)
                     /locus_tag="PLV77_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC15090.1"
                     /translation="RPLPRVGSPFPVGSGAMPKRFSILVLVALLGLAVLAGCGSDSGA
                     DSNGDPGSDQDAKPIATSDADPVLLDSNGSEFNPSKVYAETIDGVVSIRSIFGDIENS
                     PATTSVAGGSGFVLADNGEIVTNAHVISDGNGDDRKAADEVYVEFSSGDVLDAEIVGF
                     DPFADVGLLKVSDDQVEMKPLTLADSDKVAVGQPIAVIGSPFGEDQTLTTGVVSQTGR
                     SVMSLTDFQIEGAIQTDASINPGNSGGPMLDGNGHVIGISQQMKSGSGSSDGVGFGVP
                     ANTIKRSVEMLRDGGEPQYAYIGVSTEPLYPQLAEKLGLDTDKGAIVAEVVEGGPADE
                     AGIRGSDQRLIFQGARYDVGGDVIVGVNGVSIERAEDLGRLVGALKPGDTARIEVIRD
                     GEHKEIDVELENRPTAVTRP"
BASE COUNT          464 a          856 c          890 g          521 t
ORIGIN      
        1 gtcgatccag tcggccggag tccgtgaatg gatgatccgt ttcaggcctt cggcccgttc
       61 ccgcctttcc tccggggaca tggtcagcgc ccggtacatg acatcggcct ggtcctggat
      121 gtcgaaggga ttgaccgtca gggcgaactc cttcagctcc tcatgcgagc cggtgttctc
      181 ggacagcatc aacacgccgt ctctcacgtt gattaccgga gcttccttgg caaccagatt
      241 catgccgtcg aaaatcgcgt tgaccatcag caggtcgcaa tgcttgtagc gggcgacggc
      301 ctcgtcgaag tcgtcgtaga tccgcaggtc gatcggcatc cagtcggtgg ttccgtgacg
      361 gtgattgacg accgcgacca gagcctcgat ccgttcgagg tactccgcgt attcgggcac
      421 gtcctgcctt gacggctgga gatgggcgac gaaggtgacc ttctcacgga actcggggtg
      481 gtcggtcagg aaggtgtcga aggcggtgaa gccgcgcagc acgttcttcg aaagatcggc
      541 ccggtcaacc cgaaggatca ggtgttcccg ccggcgggca aggatttctt tttcgagctc
      601 ggcgacccgt tcggagtgga cagcgcgctc gagccggtcg gaatcgatcg aaagcggata
      661 ggcgcgaacc atcgtccggc cgccgggatg ggtcaccacg ttctcctcgt agtcgacctc
      721 gtagcccatc agccgccggc agcaggaaag aaagttcgaa cagtagctct gggtgtgaaa
      781 gccgatgatg tcgttggcga gcaatccgtt gaagatcgct tcgcggatcg gggtcggcag
      841 tacccgccag gagtcgggct gggtccaggg gatgtggacg aagtgatgga gcaggacgtc
      901 gggccgggcg gaccggatct tggccggcgc cgtgtacagg tggtagtcgt ggagcatgac
      961 gagcgggtcc tcgcgatcgg caatctgctc gatcacgcac ctggcgatgt cctcgttgac
     1021 cacctcgtag ccgtcgtacc aggcggtgat ttcctcacgc cggatgtccg gcgcgttgga
     1081 gaggtcccaa aggtaatgct ggatgaacca gagaatcggg ttggcgatca cgttgtagaa
     1141 gcggtcgtag gcctgcggat cgctttcgac catcagcacc cggtaatcga ccccgttgag
     1201 gttcacgtca atcggccggc cgtcgttctc ctcggtcacc accacgtctt cgctggtcat
     1261 cgcggaagcg atccacagcg cgtgacggtc cttgaccagt ccggaaaggg cggtaaccag
     1321 tccgccaccg ccccgggaaa gaacccgctc gccttccggc ccgagatcga actgggcggg
     1381 gccccggttc gagacgatga tcagtggctg ctcgtccgtg tccacgggaa ccattctatg
     1441 gcgtccggtt ttccccggac gacctgtgaa acccgtccga tcgcgggagg ctaggggcgg
     1501 gttactgcgg tggggcggtt ttccagttcg acgtcgatct ccttgtgctc gccgtcgcgg
     1561 atcacttcga tccgggccgt gtcaccgggc tttagagctc cgaccagccg gccgagatcc
     1621 tcggccctct cgatcgacac gccgttgaca ccgacgatca cgtcgccccc gacgtcatat
     1681 cgcgcgccct ggaagatcag cctctggtca ctgcccctga ttcccgcctc gtcggccggg
     1741 ccgccttcga ccacctcggc cacgatcgcc cccttgtccg tgtccagccc cagcttctcg
     1801 gccagttgcg ggtaaagcgg ctcggtgctg accccgatgt aggcgtattg cggttcaccg
     1861 ccgtcccgga gcatttccac cgagcgcttg atcgtgttcg ccgggacgcc gaaaccgacc
     1921 ccgtcactgg atcccgagcc gctcttcatc tgctggctga tcccgatcac gtgaccgttg
     1981 ccgtcgagca tcggaccgcc ggagttgccg gggttgatcg aggcgtcggt ctggattgcg
     2041 ccctcgatct ggaagtcggt aaggctcatc acggaacggc cggtctggga gacgaccccg
     2101 gtggtcaggg tctggtcctc cccgaagggg ctgccgatca ccgcgatcgg ctgaccgacc
     2161 gccaccttgt cgctgtcggc aagggtcagc ggcttcatct cgacttgatc gtcggagacc
     2221 ttgagcaggc cgacgtcggc gaaggggtcg aagccgacga tttcagcgtc aagcacgtca
     2281 ccgctggaga actctacgta gacctcgtcg gcggccttgc ggtcatcgcc gttgccgtcg
     2341 ctgatcacgt gggcgttggt gacgatctcg ccgttgtcgg cgagcacgaa tccggagccg
     2401 ccggcgaccg aggtggtggc cggcgagttc tcgatgtcgc cgaagatcga acggatcgag
     2461 acgaccccgt cgatcgtctc ggcgtaaacc ttcgacgggt tgaactcgga accattcgag
     2521 tcgagcagga ccggatcggc gtcggaggtc gcgatcggct tcgcgtcctg atcgcttccc
     2581 gggtctccgt ttgaatcggc accggaatcc gagccacagc cggcaagcac ggccagcccg
     2641 agcagggcaa ccagaaccag gatcgaaaag cgcttcggca tggcgccgga gcctaccggg
     2701 aagggcgatc cgacccgcgg aagtggtcgt g
//
LOCUS       DAOQMF010000197         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22000_length_2726_cov_3.667166, whole genome shotgun sequence.
ACCESSION   DAOQMF010000197 DAOQMF010000000
VERSION     DAOQMF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22000_length_2726_cov_3.667166"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..420)
                     /locus_tag="PLV77_04265"
     CDS             complement(19..420)
                     /locus_tag="PLV77_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC15091.1"
                     /translation="MDLDSSSQDELDVVIIGAGVSGIGAAYQLQKQHPGKSYAILESR
                     NDLGGTWDLFRYPGIRSDSDLYTFGYQFKPWTDEKAIADGPAIKAYINETAVEDCGDD
                     DGYILLEWGDGDDIVQFDFQCTECLRQIQGG"
     gene            604..1809
                     /locus_tag="PLV77_04270"
     CDS             604..1809
                     /locus_tag="PLV77_04270"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC15092.1"
                     /translation="MSWPKPSERTAELIRRCVNRILEDSEGILEAVNEASIPDEVAGV
                     GSDPVLLRNYRRVNEATVLQWAEANLREPGCEVPPYSGSEVTALTRDLVRRGLDTNAL
                     EPYRAGQNVTWQKWMEAAFDETDDPDELRELLEVSARSIFAYVDSTMMLTADRIQQQR
                     EEFNQTGAERLETVVLLLEDNPIDPHRAAARLNYSFEGTQLAAVIWKEETPDADALEV
                     GAERLKQAAGSAKLLAVHATSAALWAWVKPTREFSEIEAGLAGDGQIRFALGTPLPGL
                     DGFRRSHADASEVQRLVLRLDSGLEVASYEAMKVAALLTHDEERSKRFTAETLGELAD
                     GPVNLKETVRTYLRNQSNATRTAELLFAHRNTVLARIAKADELLPSPLSESSFEVAAA
                     LEILHWQES"
     gene            complement(1838..>2726)
                     /locus_tag="PLV77_04275"
     CDS             complement(1838..>2726)
                     /locus_tag="PLV77_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNC15093.1"
                     /translation="SAFTIAFLAAGEPARKAVQKSLDSVDADKVKADPDQAGLPLRPC
                     SMRAFMLWESHMIASSRVLVKRFFPPPAAKVVAGFEKVTGKTFPKLKPNRRFYEKPTF
                     YVANHAAVIADGDPMWWPSHTKFLDFELELAFVIGKPIADASPEEAADAVGGWFVLND
                     WSARDVQADDARENIFGPVVKSKTFANSIGCDVITPDELPDWQKATGRVRVDGEQWCE
                     GTTAGAAHDIGAMLAYASAGECLMPGDVISTGTVPGCCGLELDRWIQPGQTVELEIDG
                     IGTLTNRIDAEARPVSIAG"
BASE COUNT          501 a          871 c          868 g          486 t
ORIGIN      
        1 gattggtgtt catctctctc accctccctg gatctggcgc aagcactcgg tgcactggaa
       61 gtcgaactgg acgatgtcgt cgccatcccc ccactcgagc aggatgtagc cgtcgtcgtc
      121 gccgcagtcc tcgaccgcgg tctcgttgat gtaggccttg atcgcgggac catcggcgat
      181 cgccttttca tcggtccacg gcttgaactg gtagccgaag gtgtagagat cggaatcgga
      241 gcggattccg ggatagcgaa agagatccca ggtgccgccg aggtcgttgc gcgactcgag
      301 gatcgcgtag ctcttgcccg gatgctgctt ctgtagctgg tacgcggcgc cgatgccgga
      361 aacgccggct ccgatgatca ctacgtcaag ttcatcctgg ctgcttgaat caagatccat
      421 tttccggggt ccttctgata gatcgccgat tgagtgaatg ttcgtgccgc gaccggccca
      481 actcaacggt gaaagatcca gactttcgat ccgggctgtg cgaagtgcac aggtttacgg
      541 gaaagcaggg accggccctt cccaaataca tggttttagc ccagtcagcg ttagtctggg
      601 ccaatgtcat ggccgaagcc gtccgaacgg accgcagaac tgatccgcag atgtgtgaac
      661 cggatcctcg aggattccga ggggatcctc gaggcagtca acgaagcgag catcccggac
      721 gaggtggccg gcgtcggcag cgatccggtc ctgctgcgca attaccggcg cgtgaacgag
      781 gcaacggttc tccagtgggc agaggcaaat ctgcgggaac cgggatgcga agtcccgccc
      841 tactccggct cggaggtcac cgcgctgacc cgggacctgg ttcgccgggg actggacacc
      901 aacgccctcg agccttaccg ggccggtcag aacgtcacct ggcagaagtg gatggaagcc
      961 gccttcgacg agacagatga tccggacgag ttgcgggagc tgcttgaagt ctcggcccgc
     1021 tcgatctttg cctatgtgga ctcgaccatg atgcttaccg cggaccggat ccagcagcag
     1081 cgggaggagt tcaaccagac cggcgccgag cgactggaaa cggtggtgct cctgcttgag
     1141 gacaacccga tcgatcccca tcgcgcggcg gcacgcctca actacagctt cgaggggacc
     1201 cagctcgccg ccgtgatctg gaaggaggaa acgccggacg ccgacgcgct cgaagtcggg
     1261 gcggagaggc tgaagcaggc cgccggttcc gcgaagctgc tggccgtgca cgcgaccagc
     1321 gctgccctct gggcctgggt caagccaacc agggagttca gcgaaatcga ggcgggtctt
     1381 gccggcgatg gccagatccg cttcgcgctc ggcacacccc tccccggtct ggacggcttc
     1441 cggcgcagcc acgccgatgc ctccgaggtt cagcggctgg tgttgcggct tgattccggc
     1501 cttgaggttg ccagctacga ggcgatgaag gtggccgccc tgctgactca cgacgaagaa
     1561 cggtcgaagc ggttcaccgc ggagaccctc ggcgagctgg ccgacggccc cgtcaacctc
     1621 aaggagacgg tgcgcaccta cctccggaac cagtcaaatg cgaccaggac ggcggaactg
     1681 ctcttcgcgc accggaacac ggtcctggcg aggatcgcca aggccgacga actgctcccc
     1741 tccccgctct cggaatcctc gtttgaagtc gccgccgccc tggagatcct tcactggcag
     1801 gagagctgac cccgcctgaa ggcctgtcgc gacccgccta tccggcgatc gagacaggcc
     1861 tcgcttcggc gtcgatccgg ttggtgaggg tgccgatacc gtcgatttcc agctccacgg
     1921 tctggcccgg ctgaatccag cggtcgagtt caagcccgca acagcccggc acggtgccgg
     1981 tcgagatcac gtcgccaggc atcaggcact caccggccga agcgtaggcg agcatcgctc
     2041 cgatgtcatg agccgctccg gcagtcgtac cctcgcacca ctgttcaccg tcgacccgga
     2101 cccggcccgt agccttctgc cagtccggca gttcgtccgg ggtgatcacg tcgcagccga
     2161 tcgagttggc gaaggtcttt gacttcacca ccggaccgaa gatgttctct cttgcgtcat
     2221 cggcctgaac gtctcgggca ctccagtcgt tcagaacgaa ccagccaccg acggcatcgg
     2281 cggcctcctc cgggctggca tccgcgatcg gcttgccaat cacgaaggca agctcgagtt
     2341 cgaagtcgag gaacttcgtg tggctgggcc accacatcgg gtcgccatcg gcgatcacgg
     2401 cggcgtgatt ggccacatag aaggtcggct tctcgtagaa gcgcctgttc ggcttcagct
     2461 tcgggaaggt cttgcctgtg accttctcga aacctgccac gaccttggcg gccggcggcg
     2521 gaaagaagcg cttcacgagc acccggctgg acgcgatcat gtgtgattcc cagagcatga
     2581 aggcgcgcat cgagcagggc ctaagtggca gaccggcctg atccggatcg gctttgacct
     2641 tgtccgcgtc aaccgaatcg agcgacttct gaactgcctt gcgggccggt tcgcccgcgg
     2701 ccaggaaggc gatggtgaag gcgctg
//
LOCUS       DAOQMF010000198         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22012_length_2725_cov_3.517603, whole genome shotgun sequence.
ACCESSION   DAOQMF010000198 DAOQMF010000000
VERSION     DAOQMF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22012_length_2725_cov_3.517603"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1172)
                     /locus_tag="PLV77_04280"
     CDS             complement(69..1172)
                     /locus_tag="PLV77_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNC15094.1"
                     /translation="MIETFTLRDVGPKDGLEQAEMVAGRITDFIGKAEQSLVLALYDI
                     RLPDPVGETVAQAFRDAAARGVKVRLAYNDVPEDPDAGNFLPAVNPPPETNPEILARL
                     PIETKPIKGIPDLMHHKYMVRDEKAVWTGSSNWSVYSWTRQENVLGIIDHGEVAREYL
                     TDFEELWQKHKVERSGFGDPNPIRVGEATVRVWFTPGHGKALAQRISSKLGSARRRIR
                     IASPVLTSGPILSTLAEIANRPGLDIAGVLDEPQTDRVYEQWATTGSKWKIPLLTQVV
                     ETIPFSGKRSVPYGTGTVRDFMHAKVTVADDTVFFGSFNLSRSGEANAENMVEIEDPG
                     IAGKMAKFIDETRALYPRSTVPDRRGPRKGERT"
     gene            complement(1169..1600)
                     /locus_tag="PLV77_04285"
     CDS             complement(1169..1600)
                     /locus_tag="PLV77_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNC15095.1"
                     /translation="MSAATAEPKTDRKIRVVVAKPGLDGHDRGAKIIARALRDSGMEV
                     IYTGLHQTPEQIVETVIQEDADAIGVSILSGAHMTLVPKILELLEAEGIDDVLITVGG
                     TIPNKDIDALKEMGVSGVFTPGAPTDDIVNFIRTGVSRREL"
     gene            complement(1624..>2725)
                     /locus_tag="PLV77_04290"
     CDS             complement(1624..>2725)
                     /locus_tag="PLV77_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNC15096.1"
                     /translation="SGTIQTDILKEYIAQKEWCFPVEPAMRLVTDMVEYCTDHMPRWH
                     PISISGYHIREAGATAAQELAFTLKDGFTYVERAIERGIDVDAFAPRLSFFFNAHIDF
                     FEEIAKYRAARRIWARELKETYGAKKPESMRLRFHTQTAGVSLTAQQPLNNIVRTAIE
                     ALAGVLGGTQSLHTNSFDEALALPTEEAVRVALRTQQIIADETGVTNTPDPLGGSYFV
                     ESLTDELEADAYDYFRRIDELGGMVEAVRQNFPQREIGEAAFDFQRRVDDGEFTIVGV
                     NQYVTEEEEVPLLHIDPALETKQVERLAATRAKRDSAEVESALTALKDAAAGEANLMY
                     PILDAARVHTTEGEMIEAMQQVFGTYTETPVF"
BASE COUNT          413 a          849 c          935 g          528 t
ORIGIN      
        1 gcccggcacc accatgaagt ccatggctcc gtcgagggcc tcggcggcct tgccccggag
       61 cgggagcatc aggttcgctc gcccttgcgc gggccgcgcc ggtccggcac ggtgctgcgc
      121 gggtagagcg ccctggtctc gtcgatgaat ttcgccatct tcccggcgat acccggatcc
      181 tcgatttcca ccatgttctc ggcgttggct tccccagagc gggaaaggtt gaaggaaccg
      241 aagaagacgg tgtcgtcggc gaccgtgacc ttggcgtgca tgaagtcgcg gacggtgccg
      301 gtgccgtagg gaaccgaccg cttgccgctg aacggaatcg tttccaccac ctgggtgagc
      361 agcggaatct tccacttaga gccggtggtc gcccactgtt catagacgcg gtccgtctgt
      421 ggttcgtcga gcaccccggc gatgtccagt ccgggccggt tcgcgatctc ggccagggtg
      481 ctgaggatcg ggccggaagt caggaccggc gaggcgatcc ggatccggcg gcgggccgaa
      541 ccgagcttcg acgagatccg ttgggcaagc gccttgccgt gaccgggggt gaaccagacc
      601 cggaccgtgg cctcccccac cctgatcgga ttgggatcac caaagccgga tcgctcgacc
      661 ttgtgctttt gccagagttc ctcgaagtcg gtcaggtatt cgcgggcgac ctcgccgtga
      721 tcgatgatcc ccaacacgtt ctcctgccgg gtccaggagt agaccgacca gtttgaggag
      781 ccggtccaga ccgccttctc gtcccgcacc atgtacttgt ggtgcatcag gtcggggatg
      841 cccttgatcg gcttcgtttc gatcggcagt ctggccagga tctccggatt ggtctccggc
      901 ggcgggttca cggccggcag gaaattgccg gcatcgggat cttccggcac gtcgttgtag
      961 gcgagtcgca ccttgactcc ccgggccgcg gcatcccgga aggcctgtgc cacggtctcc
     1021 ccgaccgggt ccggcagccg gatgtcataa agggcgagga ccaggctctg ctcggccttg
     1081 ccgatgaagt cggtgatcct cccggccacc atctccgcct gctccagccc gtccttcgga
     1141 ccaacgtcac ggagggtgaa ggtttcgatc acagctcgcg gcggctgacg ccggtgcgga
     1201 tgaagttgac gatgtcgtcg gtcggggcgc cgggagtgaa gactcccgag acgcccatct
     1261 ccttgagcgc gtcgatgtcc ttgttcggga tcgtgccgcc gaccgtgatc aggacgtcgt
     1321 cgatgccttc ggcttcgagc agttcgagga tcttcgggac cagggtcatg tgggcaccgg
     1381 agaggatcga gacaccgatc gcgtcggcgt cctcctgaat caccgtttcc acgatctgct
     1441 ccggggtctg gtgaaggccg gtgtagatga cctccatgcc ggagtcgcgc agggcgcggg
     1501 cgatgatctt ggctccccgg tcgtggccgt cgaggccggg cttggccacg accacgcgga
     1561 tcttgcggtc ggtcttcggt tcggcagtgg cggcgctcat cagctgaaaa cctgctttcg
     1621 gatctagaag accggagtct ccgtgtaggt gccgaatacc tgctgcatcg cctcgatcat
     1681 ctcgccttcg gtcgtgtgga cccgggcggc atcgaggatc gggtacatca ggttggcctc
     1741 gcctgccgcg gcatccttga gggccgtcag ggcggactcg acttcagccg agtcgcgctt
     1801 tgccctggtc gcggcgagac gctcgacctg cttggtctcg agggcgggat cgatgtgaag
     1861 gaggggaacc tcctcttcct cggtcacgta ctgattgacc ccgacgatcg tgaactcgcc
     1921 gtcgtccacc cggcgctgga agtcgaaggc cgcttcaccg atctcgcgct gggggaagtt
     1981 ctggcggacc gcttcgacca tgccgcccag ttcgtcgatc cggcggaagt agtcgtaggc
     2041 gtcggcttcc agctcgtcgg tcagcgactc gacgaagtac gaaccgccga gcggatcggg
     2101 cgtgttggtg actccggttt cgtcggcgat gatctgctgg gtccgcagcg cgacccggac
     2161 cgcttcctcg gtcggcaggg cgagggcctc gtcgaaggag ttggtgtgaa gcgactgggt
     2221 gccgccgagc accccggcca gggcctcgat cgcggtccgg acgatgttgt tcagcggctg
     2281 ctgtgcggtc agcgagaccc cggcggtctg cgtgtggaag cgaagccgca tcgactcggg
     2341 cttcttcgct ccgtaggtct ccttgagctc acgggcccag atccggcggg cggcgcggta
     2401 cttggcgatt tcctcgaaga agtcgatgtg ggcgttgaag aagaacgaaa gtctgggcgc
     2461 gaaggcgtcg acgtcgatgc cgcgctcgat cgcccgttcg acgtaggtga acccgtcctt
     2521 gagggtgaag gcgagttcct gcgcggcggt agccccggcc tcgcggatgt ggtacccgga
     2581 gatcgagatc ggatgccagc gcggcatgtg gtcggtgcag tactcgacca tgtcggtgac
     2641 caggcgcatc gccggctcga ccggaaagca ccactccttc tgggcgatgt actccttgag
     2701 gatgtcggtc tggatcgtgc ccgag
//
LOCUS       DAOQMF010000199         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22038_length_2723_cov_4.075337, whole genome shotgun sequence.
ACCESSION   DAOQMF010000199 DAOQMF010000000
VERSION     DAOQMF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22038_length_2723_cov_4.075337"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLV77_04295"
     CDS             complement(<1..903)
                     /locus_tag="PLV77_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNC15097.1"
                     /translation="MPEENPALFLIDGNSMAYRAFFALPDSMATADGRPTNAIFGLAS
                     MMVKLISDYRPRQIAVAWDAGMSGREVVYPEYKAQRPPKPDLLRQQWPHLLEMVEAFG
                     YTNVKVDGYEADDVIATLTKQASMVDIPVVVVTGDRDAYQLVTDTVQVMSTSRGVTET
                     KLYDREAVKERYGVYPELVTDLMGLRGDTSDNIPGVPGIGEKTAAQLLDQFGSLEAIL
                     AGVDEVSGKKRKENLIEFAEDARISKQLATMDFEVETGLDVDELMAQTPDREGLRAFM
                     TEFELRAAMKRLEEALEDEEIPGVS"
     gene            970..2421
                     /locus_tag="PLV77_04300"
     CDS             970..2421
                     /locus_tag="PLV77_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNC15098.1"
                     /translation="MPPVSAQYSVTVRVELDARQEPLGKLTAAVAEAGGQLVGVDLIP
                     GAGPEGKRVREFTIDAVDQPHWERILRALGSIRGARVMEYTDRTMQMHRGGKVSVENK
                     YPLKTRDDLSMAYTPGVARVCTAINQDRQLAFEYTIKKNTVAVVSDGTAVLGLGDIGP
                     EAAMPVMEGKAMLFKEFAQVDAFPICLNTTDTDEIVRAVEMIAPTFGGINLEDISAPR
                     CFEIEDRLKESIDIPVFHDDQHGTAVVTMAALFNALKIIDKPIEELRVLMVGLGAAGV
                     AVTKMMLAAGLTHVVGCDRKGALSVTREDYQAGELSGIKKWFADNSNPDHLLGTPDEV
                     IEGMDVFVGLSGPGIIQPESLGKMNRDPIVFAMSNPDPEVAPEEAAKYVRIMATGRSD
                     YPNQINNVLCFPGIFRGALDAGAPQITEEMKMAAARGIADVVGDDLSEDYIIPSVFDR
                     DVAPRVAEAVIQEAKRAGDARFNVETGAFTALG"
     gene            complement(2484..>2723)
                     /locus_tag="PLV77_04305"
     CDS             complement(2484..>2723)
                     /locus_tag="PLV77_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15099.1"
                     /translation="GSPVAIECRTEGTVEFDESVVVFGRVVHLAISEAVLDGDHPDIG
                     KLAPMARLGRNEWSEIGKVHRIDRIPLDRARRNGS"
BASE COUNT          481 a          903 c          861 g          478 t
ORIGIN      
        1 ggaaacgccg gggatctctt cgtcctccag ggcttcttcg agccgtttca tcgcggcccg
       61 cagctcaaac tcggtcatga aggcgcgaag gccctcacgg tccggggtct gggccatcaa
      121 ctcatcgaca tcgagcccgg tttcaacttc gaagtccatc gtcgcgagct gcttggagat
      181 gcgggcatcc tcggcgaact cgatcaggtt ctccttgcgc ttcttgcccg agacctcgtc
      241 gacgccggcg agaatcgctt ccagtgagcc gaactggtcg agcagctgag cggcggtctt
      301 ctcgccgatc cccgggactc ccggaatgtt gtcggaggtg tcaccgcgaa gccccatcag
      361 gtcggtgacc agttccgggt agacgccgta tcgctccttg accgcctcgc ggtcgtagag
      421 cttggtttcg gtcacgcccc tggatgtgct catgacctgg accgtgtcgg tcaccagctg
      481 gtaggcgtca cggtcaccgg tgaccacgac caccggaatg tcaaccatcg aggcctgttt
      541 ggtcagggtc gcaatgacgt catcggcctc gtagccgtcg accttgacgt tcgtgtatcc
      601 gaacgcttcg accatttcca gcagatgagg ccactgctgg cggagcaggt cgggcttggg
      661 cggacgctgg gccttgtact cgggatagac gacctcgcgg ccggacatgc cggcgtccca
      721 ggcgaccgcg atctgccttg gccggtaatc cgagatcaac ttgaccatca ttgatgccag
      781 accgaaaatc gcgttggtcg ggcggccgtc ggcggtcgcc atggagtccg gcagggcgaa
      841 gaaggcccgg taggccatgc tgttgccgtc gatcaggaaa agcgctgggt tctcttcggg
      901 caccgaccga tcataggtct ttcgccagtc gggacagggc gatctgctgc gccctcgtaa
      961 gatgagccaa tgccacccgt ttccgctcaa tacagcgtca ccgtccgcgt cgaactcgat
     1021 gcccgccagg agcctctcgg caagctgacc gcagccgtag ccgaggccgg cggccaactc
     1081 gtaggcgtgg acctgattcc cggcgccggt ccggaaggca agcgggtccg cgagttcacg
     1141 atcgacgcgg ttgaccagcc ccattgggag cggatcctgc gtgctctcgg ttcgatccgg
     1201 ggcgcccggg tcatggagta cacggaccgc accatgcaga tgcaccgggg cggcaaggtt
     1261 tcggtcgaga acaagtaccc gctgaagacc cgcgacgatc tttcgatggc ctacaccccg
     1321 ggtgtcgccc gggtctgcac ggcgatcaac caggaccgcc agctcgcctt cgagtacacg
     1381 atcaagaaga acaccgtggc ggtcgtctcc gacggcaccg cggtgctcgg cctcggcgac
     1441 atcggccccg aggcggcaat gcccgtgatg gagggcaagg cgatgctctt caaggagttc
     1501 gcccaggttg acgctttccc gatctgcctc aacacgaccg acaccgacga gatcgtccgt
     1561 gcagtcgaga tgatcgcccc caccttcggg ggaatcaacc tcgaggacat ctccgccccg
     1621 cgctgcttcg agatcgagga ccggctcaag gaatcgatcg acatcccggt tttccacgat
     1681 gaccagcacg gcaccgcggt ggtgaccatg gccgcgctgt tcaacgcgct gaagatcatc
     1741 gacaagccga tcgaggagct gcgggtgctg atggtcggcc tcggcgccgc cggcgtggcg
     1801 gtgaccaaga tgatgcttgc cgccggcctc acccacgtgg tcggctgcga ccgcaagggc
     1861 gccctttcgg tcactcgcga ggattaccag gccggcgagc tgagcgggat caagaagtgg
     1921 ttcgccgaca actcgaaccc ggaccatctg ctcggcactc cggacgaggt gatcgaaggc
     1981 atggacgtgt tcgtcggcct gtcaggcccg ggcatcatcc agcccgagtc gctgggcaag
     2041 atgaatcggg atccgatcgt cttcgccatg tcgaatccgg acccggaggt tgctcccgag
     2101 gaggccgcca agtacgtgcg gatcatggcc accggccgct ccgactaccc gaaccagatc
     2161 aacaacgtgc tctgtttccc gggaatcttc cgcggcgcac tcgacgccgg cgcgccacag
     2221 atcaccgagg agatgaagat ggccgccgcc cggggcatcg ccgacgtggt cggggacgac
     2281 ctctccgagg actacatcat cccctcggtc ttcgaccgtg acgtcgcacc ccgggtagcc
     2341 gaagcggtga tccaggaagc caagcgggcc ggcgacgcgc gcttcaacgt cgagacgggc
     2401 gccttcaccg ccctgggctg agtctccctg cggcgagaac ccgctttgcg ccacataccg
     2461 gcacaaagtg ggcattcgat ggtctagctc ccgtttcggc gcgcccgatc caggggaatc
     2521 cggtcgatcc ggtggacctt gccgatctcc gaccactcgt tgcggccgag acgggccatc
     2581 ggggcgagct tcccgatgtc gggatgatct ccgtccagca ccgcttcgct gattgccaga
     2641 tgaaccaccc ggccgaagac gaccaccgat tcgtcgaact cgacggtgcc ctcggtccgg
     2701 cattcgatcg ccaccggtga acc
//
LOCUS       DAOQMF010000200         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22242_length_2708_cov_2.801734, whole genome shotgun sequence.
ACCESSION   DAOQMF010000200 DAOQMF010000000
VERSION     DAOQMF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22242_length_2708_cov_2.801734"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLV77_04310"
     CDS             complement(<1..549)
                     /locus_tag="PLV77_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15100.1"
                     /translation="MNSVSRVVFRIVAVAALLFAGLFAASSAQAAGTPDLQLSASSSN
                     PLYGENGNVSVTAALGSGQPKGYNLSFRVVLPAGISYAGGAAYAPTVLSNSPTTGKTT
                     LIFSNISDLVEDSSQSVSFQVAHDQAQYDVGNTYDIQYEAFLNTDPRIMPAFNADGTA
                     KPASYTGSGSATTTSKINAIKIS"
     gene            complement(905..>2708)
                     /locus_tag="PLV77_04315"
     CDS             complement(905..>2708)
                     /locus_tag="PLV77_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNC15101.1"
                     /translation="TDTLPAGVTYVDGAPGCSAAGQVVTCTLNPIAAGGSRQTGITVS
                     VSPSATGVITNAAHVTGNENDPDPSNNDDSVDTPVKANADVEITKTASPANPVSGDTV
                     TYTLKAKNNGPDPAANVVVTDVLPAGVSFVSADSPCTEASGTVTCALGTLAAGEEVTL
                     EVKVTVDHWGDTDPSADHLLDVQKVENQIDLNPGEQKTIQVTCPSGYFATDGSVRIDE
                     IDQGTGDWTAPQVLENRASSLDTWQGTVRNTAAGRAQAKIFAVCVRKTTGDENGHHHN
                     LLVSAPVTVTDSLVPGNNTKVLQCGPGQVAIQPGFIADKAGHLVYSQPEGNGWKFIYK
                     VEEGGTASFSIRCMDRQVDVVNGHTHDLKLQRISTEVEVAPGTVNEAQLTCPDGSKGI
                     VAGWDLDDGLVSLGNDPRPVTRAFKLYNPTDHTLSARLSLLCLGLRTGGERVPSKTII
                     NTANISTSSTESETGNNGSSAGINVNGIDNTPIDNDPDPDKPHPNNPIAKTIVGKGVS
                     YSASGVSFSLKCSGTCGGFAKLTTLRKLKVKGKKYGRGTVLARRQYFIGKAGTKKVKL
                     TLTGKGRKILKSGKAHRALLSISGGTRKVVKVGH"
BASE COUNT          437 a          777 c          936 g          558 t
ORIGIN      
        1 gctgatcttg atcgcgttga tcttcgaagt ggtcgtggca gaaccgctac cggtgtagct
       61 cgctggcttg gcggtgccgt ccgcgttgaa ggccggcatg atccgcggat cggtgttgag
      121 gaaagcctcg tactggatgt cgtaggtgtt gccgacgtcg tactgcgcct gatcgtgggc
      181 gacctggaag ctgaccgact gggacgaatc ctcgaccagg tcggaaatgt tgctgaagat
      241 cagggtggtc ttgccggttg tcggcgagtt gctcaggacc gtgggtgcgt aggcggcacc
      301 gccggcgtag gagatcccgg ccggcagcac gacacggaag gacaggttgt agcccttggg
      361 ctgaccggaa ccgagggcgg cggtgaccga gacgtttccg ttttcgccgt aaagcggatt
      421 cgacgaggat gccgacagct gaaggtcggg cgttcccgcg gcctgggccg aggatgcggc
      481 gaaaagaccc gcgaaaagaa gggcggctac ggcgacaatt cgaaatacga cacggctgac
      541 agaattcaat ttttctccga tacttcgtgg acaccctggt tggggtccga tccattgctt
      601 tgatggcctg tgtgaaacgg tgggaaagcc tcggaggcca ggtcaactgc cgcaagaatt
      661 gagaattgca gttgacagag ctttttcaga atcccccggc cgcctgccgg ttctcaaagg
      721 gaatcaagcc cgtctcccgt gttcagattg gctgtcaata ataggaacaa tccgtagctt
      781 gtcaagttac ggtggcgcca gaagaaaaat tgtaggtgcg cgggcaacca aaatcccgct
      841 agttgcagca aaaagccccg gtcttgtgac cggggctttt actccctggc agtaagtctc
      901 aggctcagtg cccgactttg actacctttc tggtccctcc agaaatgctg agcagagccc
      961 tgtgggcctt cccgctcttc aggatcttgc ggcccttgcc ggtcagggtc agtttgacct
     1021 tcttcgtgcc ggccttgccg atgaagtact ggcgcctggc gagaactgtg ccgcggccgt
     1081 acttcttgcc cttgaccttc agcttcctga gcgtggtcag cttggcgaac ccgccacagg
     1141 tacccgagca cttgaggctg aagctgacac ctgaagcgga gtagctgacg cccttgccga
     1201 cgatcgtctt ggcgatcggg ttgttcgggt gcggcttgtc cggatccgga tcgttgtcaa
     1261 tcggggtgtt gtcgatcccg ttcacgttga tcccggccga agagccgttg ttgccggttt
     1321 ccgactcggt cgagctggtc gagatgttgg ccgtgttgat gatcgtcttg gacggcaccc
     1381 gctcaccacc ggtacggagg cccaggcaga gcagactgag gcgggcactc agcgtgtggt
     1441 cggtcgggtt gtagagcttg aaggcccggg tgaccggacg cggatcgttg ccgagcgaaa
     1501 cgagaccgtc gtcgaggtcc cagccggcga cgattccctt gctgccgtcc gggcaggtga
     1561 gctgcgcctc gttgaccgtg cccggtgcaa cctcgacctc ggtcgagatg cgctggagct
     1621 tcaggtcatg ggtgtgaccg ttcacgacat ccacctggcg gtccatgcag cggatcgaga
     1681 aggaggcggt gccaccctct tcgaccttgt agatgaactt ccagccgttg ccttcgggct
     1741 ggctgtagac caggtgaccg gccttgtcgg cgatgaagcc gggctggatc gcaacctggc
     1801 cggggccgca ctgcagcacc ttggtgttgt tgcccgggac cagcgagtcg gtcacggtga
     1861 ccggggcgct gaccagcagg ttgtggtggt ggccgttctc gtcgccggtg gtcttcctga
     1921 cgcagaccgc gaagatcttg gcctgggcgc gaccggccgc ggtgttgcgg accgtgccct
     1981 gccaggtgtc cagcgagctg gcccggtttt cgaggacctg cggagcggtc cagtcaccgg
     2041 tgccctgatc gatttcgtcg atccggacgg aaccgtcggt cgcgaagtaa ccggaggggc
     2101 aggtgacctg aatggtcttc tgctcgccgg gattcaggtc gatctggttc tccaccttct
     2161 ggacgtccag cagatgatcg gccgaggggt cggtgtcgcc ccagtggtcg accgtgacct
     2221 tgacttcgag cgtcacttcc tcgcccgcgg ccagcgtgcc aagggcacag gtgaccgtgc
     2281 cggaggcctc ggtgcagggc gagtcggcgc tgacgaagct gacgccggcc gggagcacgt
     2341 cggtcacgac gacgttggcg gccgggtcgg gaccgttgtt cttcgccttc agcgtgtagg
     2401 tgaccgtgtc acccgacacg gggttggccg gcgaagcggt cttggtgatc tccacgtccg
     2461 cgttcgcctt gaccggggtg tcaaccgagt cgtcgttgtt cgacgggtcc ggatcgtttt
     2521 cgttgccggt gacgtgggcg gcgttggtga tcacgccggt cgcgctcggg gagacgctga
     2581 ccgtgatccc ggtctggcgg gaaccaccgg cggcgatcgg gttgagcgtg caggtcacga
     2641 cctggccggc cgcgctgcag cccggggcgc catcaacgta ggtgactccg gcgggcaggg
     2701 tgtcggtg
//
LOCUS       DAOQMF010000201         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22609_length_2684_cov_3.850133, whole genome shotgun sequence.
ACCESSION   DAOQMF010000201 DAOQMF010000000
VERSION     DAOQMF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22609_length_2684_cov_3.850133"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLV77_04320"
     CDS             complement(<1..345)
                     /locus_tag="PLV77_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNC15102.1"
                     /translation="MSGEDAYAKAGVSQSDADLAVSRLVAALATANIGRPTRQVDLKG
                     HYAAVMRLDNRTGIALSTDGVGTKLLVAEELGRFDTVGIDCIAMNVNDIICVGAEPVA
                     MLDYLAVEKADPE"
     gene            complement(373..1164)
                     /locus_tag="PLV77_04325"
     CDS             complement(373..1164)
                     /locus_tag="PLV77_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNC15103.1"
                     /translation="MILGLVQGPAELLPISSSAHLAMLPGLLRWDPDELDPELRKSFE
                     VALHGGAALALLITRRGEIRDEIGEFDLRRASVLLLSFLPAAAVGYGFERVIESRFGG
                     PRGTATGLILGGLAMALADRRPQERGRGEADWKDGLALGIGQAAALIPGVSRNGATLT
                     AARLRRFSRDQANLLSRTVALPVILGAVGLKGVRLARRGISRRQARWLAIGTVTSFAS
                     TMAATDLIRMVERDRALAPYAAYRIAFALLILARLAADRGDRAAE"
     gene            1249..>2684
                     /locus_tag="PLV77_04330"
     CDS             1249..>2684
                     /locus_tag="PLV77_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNC15104.1"
                     /translation="MRRPDFHLRRYQLMQMAGDGLLVALSYFLAFQLRFLDDPGTIPE
                     RYVDLFLQSVGFVIVGKLIVFYLFGLYQKWWRFVGGRDIARIVQAVSVSSLLLVVLFT
                     VIQPFPHDLPRSVAVTDFLLTLFLIAGARLATRLFKERPNKGNRIARGREVLVVGAGS
                     GGQMVVRELRINPNLGETAIGFVDDNTEMRGMRIQGIRVLGTTDEIETILDETEPDEV
                     MIAIPSAPGVLRARVVAACRERQIRVRTLPTVFELLRGGVQLSQQLREVQVEDVLGRD
                     PVTMELARVGAYLEDQVVMVTGAGGSIGKELCRQIGLVGPRLLVMLDHAEDNLFQIER
                     EMVAERHFTRVEAVLADCKETERIFEVMQRFKPNIVFHAAAYKHVPLMESNPLEAVRN
                     NSFATRITADCAAAAGAERFVLISTDKAVNPQTVMGASKAMAEWAVAAADAHHANTRF
                     VSVRFGNVLGSSGSVVPICRYSDSTSTVT"
BASE COUNT          431 a          905 c          906 g          442 t
ORIGIN      
        1 ctccgggtcg gccttctcga ccgcgaggta atcgagcatg gcgaccggtt cggctccgac
       61 gcagatgatg tcgttcacgt tcatcgcgat gcagtcgatc ccgaccgtgt cgaaacggcc
      121 aagttcctcg gcgaccagca gcttggtccc gaccccgtcc gtcgaaagcg cgatcccggt
      181 gcggttgtcg agccgcatca ccgcggcgta atggcccttg agatcgacct gccgggtggg
      241 gcggccgatg tttgcggtgg ccagggcggc gaccagccgg ctgaccgcga ggtccgcgtc
      301 ggactggctg actcccgcct tcgcgtatgc gtcttcgccg ctcatcgcag ttgattcaac
      361 attagaaggc ggtcactcgg cagcacggtc accgcgatcg gcggccagtc tcgccaggat
      421 cagcagggcg aaagcgatcc ggtaggccgc gtaaggggcg agtgcccggt ccctttcgac
      481 catccggatc agatcggtcg cggccatcgt cgaggcaaag gaagtaacgg tgccgatcgc
      541 cagccagcgc gcctgccggc gtgagatgcc gcgccgggca agccgtaccc ccttcaggcc
      601 gaccgccccg aggatcaccg gcagggcgac ggtgcgggag agcagattgg cctgatcacg
      661 ggagaagcgg cgcaaccggg cggcggtcag ggtggcgccg ttgcgtgaga cgccgggaat
      721 cagcgccgcc gcctggccga tcccgagggc gagcccgtcc ttccagtccg cttccccccg
      781 gccccgctcc tggggccggc gatcggccag ggccatcgcc agccccccga ggatcagccc
      841 ggtcgcggtg ccccgcggcc cgccgaagcg ggattcgatg acccgttcga atccataacc
      901 gacggcagcc gcaggcagga aggaaaggag caggaccgag gcgcgacgga ggtcaaactc
      961 accgatttca tcccggatct ctccgcgccg ggtgatcagc agcgccagtg ctgcgccccc
     1021 gtgaagggcg acttcgaagc tcttgcgcag ttccgggtcg agctcatccg gatcccaccg
     1081 gagcaggccg gggagcatcg ccagatgggc cgaggaggag atcggcagca gttcggccgg
     1141 accctgaacc agcccgagga tcaccgcatg gccagttgcg cgtcggctgt caccggaatc
     1201 ggaaatgaga gcggggatgc tacccgcggg gtagaatccg tggcttcaat gcgacgccca
     1261 gactttcatt tgcgccggta ccagctgatg cagatggccg gtgacggcct gctcgtcgcg
     1321 ctttcctact tcctcgcctt ccagcttcgc ttcctcgatg acccggggac gatccccgag
     1381 cgttacgtcg acctgttcct gcagtcggtc ggtttcgtga tcgtcggcaa gctgatcgtc
     1441 ttctacctgt tcgggctcta ccagaagtgg tggcgtttcg tgggcggtcg tgacatcgcc
     1501 cggatcgtcc aggcggtttc ggtttcgagc ctcctgctgg tcgtgctgtt cacggtgatc
     1561 cagcccttcc cgcatgacct gccccgctcg gtcgcggtga ccgactttct cctcaccctc
     1621 tttctgatcg ccggcgcccg gctcgccacc cggctcttca aggaacgtcc gaacaagggc
     1681 aaccggatcg cccggggccg cgaggtgctg gtggtcgggg ccggttccgg tggtcagatg
     1741 gtggtccgtg aactgcggat caacccgaac ctcggcgaga ccgcgatcgg cttcgtcgat
     1801 gacaacacgg agatgcgcgg gatgcgcatc cagggcatcc gggtgctcgg caccaccgac
     1861 gagatcgaga cgatcctcga cgaaaccgaa ccggacgagg tgatgatcgc gatcccctcc
     1921 gcgcccggtg tgctgcgggc gagggtggtg gccgcctgcc gtgaacggca gatccgggtc
     1981 cgcaccctgc cgaccgtgtt cgagctgctg cggggcggcg tccagctaag ccagcagctg
     2041 cgcgaggttc aggtcgagga cgtgctcggc cgcgatccgg tgaccatgga gctggcccgg
     2101 gtcggcgcct acctcgagga ccaggtcgtg atggtgaccg gcgcgggcgg ctcgatcggc
     2161 aaggagctct gccgccagat cgggctggtc ggaccgcggc tgctggtcat gctcgaccac
     2221 gccgaggaca acctcttcca gatcgaacgc gagatggtgg ccgagcgcca cttcaccagg
     2281 gtcgaggcgg tcctggccga ctgcaaggag accgagcgga tcttcgaggt gatgcagcgc
     2341 ttcaaaccca acatcgtctt ccacgccgcc gcctacaagc acgtgccgct gatggagtcg
     2401 aacccgctcg aggcggttcg caacaactcg ttcgcgaccc ggatcaccgc cgattgcgcc
     2461 gccgcggccg gggccgagcg cttcgtcctg atctcgaccg acaaggcggt caacccgcag
     2521 accgtgatgg gtgcctcaaa ggcaatggcc gagtgggcgg tcgccgccgc cgacgcgcac
     2581 cacgcgaaca cgcgcttcgt ctcggtccgt ttcggcaacg tgctcggatc ctccggcagc
     2641 gtggtgccga tctgccggta ttccgacagc acctcgacgg tcac
//
LOCUS       DAOQMF010000202         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22733_length_2677_cov_3.534706, whole genome shotgun sequence.
ACCESSION   DAOQMF010000202 DAOQMF010000000
VERSION     DAOQMF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22733_length_2677_cov_3.534706"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLV77_04335"
     CDS             <1..311
                     /locus_tag="PLV77_04335"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC15105.1"
                     /translation="YGTDGNRNGVGSSRKLNPPGPVPAFEETVTEFEPHSRIVYTVTK
                     GTPLNHHLGTITFAPTDSGTHMEWNIEIGTRLPGLDFVIAKVLVRNIGGGLDRMTIPA
                     "
     gene            complement(321..989)
                     /locus_tag="PLV77_04340"
     CDS             complement(321..989)
                     /locus_tag="PLV77_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC15106.1"
                     /translation="MNTSIAEDSVSPEELTEAVSERRGWKLYQVIRLILRPLRWWARV
                     ESKGLEYLPATGPALIVSNHDSWLDPLAIIEVMMWKKRQLRFLAKHTLWKFRPMAWIL
                     DQAAQIPIRRGEGDTGALDAAAVAVERGEMIGIFPEGTLSRGTVLRARKGVSRLARAC
                     PGVPVVLVAVTGSSDLKRFPKRPRVKVEFFDPAGGQLRPDEDAQEFSQRLLDEIRAKA
                     PRVK"
     gene            complement(1271..2080)
                     /locus_tag="PLV77_04345"
     CDS             complement(1271..2080)
                     /locus_tag="PLV77_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15107.1"
                     /translation="MRAASVRENSRLRNLLREIGEIFAGIFHVYRAWWVDILVLSFCI
                     FVPIGLLDAADNQALESIGPGHDLTATALGIAALVITATALLGEVFLAGAVGLSLIHA
                     EDGRPPSLRFIARHLSYGRLIAVDILFVLAVALGLVFLVVPGMAAFAYLALAGPVVEI
                     EDRGIASSFRRSFQLVRGRFWLVFLILFPIEAVGGAVGQGSAAICETLFGHTFFAEGI
                     AEALADIAISPLFAIASVLLARKLIMARDGIELPGPGLRDLQPAPSGKPPA"
     gene            complement(2137..>2677)
                     /locus_tag="PLV77_04350"
     CDS             complement(2137..>2677)
                     /locus_tag="PLV77_04350"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15108.1"
                     /translation="DAVPEQEATYASDGFAPAYGNARFSAEARDLPDPEPEAGILPSS
                     SVPFDQLVAFDAAHYFGPRPAFLRAWIKGEGRQSLVATDGTSITGFVASRPTRTGNRI
                     GPFFAANATIAGNLLLELATRLTGPVSIDAPEPNQAASDLFTSLGMKRSFPTIRMYRG
                     LPPDLPLDRIFGITTLELG"
BASE COUNT          501 a          858 c          912 g          406 t
ORIGIN      
        1 cctacggcac cgacggcaac cgcaacggag tcggttcctc gcggaaactg aatccgcccg
       61 ggccggttcc ggccttcgag gagactgtga ccgaattcga gccgcacagc cggatcgtct
      121 acacggtgac caagggcacc ccgctcaacc atcacctcgg cacgatcact ttcgccccga
      181 ccgattccgg aacccacatg gaatggaaca tcgagatcgg cacccgcctg cccggccttg
      241 acttcgtgat cgccaaggtg ctggtcagga acatcggcgg cggactcgac cggatgacga
      301 tcccggcctg aaccccggcg ctacttgacg cgaggcgcct tggcgcggat ctcgtcgagc
      361 aggcgctgcg agaactcctg cgcgtcctca tccggtctca gctgcccccc ggccgggtcg
      421 aagaactcga ccttcacccg gggccgcttg gggaaacgct tcaggtcact gctgccggtc
      481 accgcgacca gcacgaccgg gactcccggg caggcccggg ccagccggga gactcccttg
      541 cgggctcgca gcaccgtgcc ccgtgaaagc gttccttcgg ggaagatgcc gatcatctca
      601 ccgcgttcga cggcaactgc tgcggcgtcc agggcgccgg tgtcaccctc gccgcgccgg
      661 atcgggatct gcgcggcctg atccaggatc caggccatcg gcctgaactt ccagagcgtg
      721 tgcttggcga ggaaacgcag ttgacgcttc ttccacatca tcacctcgat gatcgccagc
      781 gggtcaagcc aggaatcgtg gttcgagacg atcagcgcgg gaccggtcgc cggcaggtac
      841 tcgagcccct tcgattccac ccgtgcccac cagcgcaacg gccgcagaat caggcggatg
      901 acctggtaga gcttccagcc gcgtcgctcg gaaacggcct cggtcagctc ttctggcgag
      961 accgaatcct cggcgatcga tgtgttcatc ggcttcccgc cgtgccttgc ggattcatga
     1021 acatcggact ctagagcctg agactctgaa gactgcggtg ttcgcggaaa tcacccgaaa
     1081 cgggcggtga acaggtcgcg gcggcactcg ccggatcgat ccgaaaggcc ggtgcgaagg
     1141 ctccggcagc agccagcgcc gcacctcgcc cgagtagcgc gcgtctggtc agcggtcgcc
     1201 cgctcacggc ctcccctttc tcccgctcca cccgcggcag tctagcttgc ggtcccggtg
     1261 ctgctgaaga tcaggccggg ggcttcccgg acggggcggg ctggaggtcc cggaggcccg
     1321 gaccgggaag ttcgatgcca tccctggcca tgatcagctt tctggccagg agaaccgaag
     1381 cgatcgcgaa cagtggcgaa atcgcgatgt cggccagcgc ctcggcgatg ccttcggcaa
     1441 agaaggtgtg gccgaagagc gtctcgcaga tcgcggcaga gccctgcccg acggcgccgc
     1501 cgaccgcctc gatcggaaag aggatcagga agacgagcca gaagcgtccg cggacgagct
     1561 ggaagctgcg tctgaacgag ctcgcgatgc cacggtcctc gatttcgacc accggcccgg
     1621 ccagcgcgag gtaggcgaag gcggccatgc cgggaaccac cagaaagacc agcccgaggg
     1681 ccaccgccag cacgaagagg atgtcgacgg cgatcagccg gccgtagctg aggtggcggg
     1741 cgatgaagcg aagcgacggc ggccggccgt cttccgcatg gatcagcgag agcccgacgg
     1801 caccggcgag aaagacctcg ccgagcaggg ccgtagcggt gatgaccagg gccgcgattc
     1861 ccagggccgt cgccgtcagg tcgtgacccg ggccgatcga ttcgagcgcc tggttgtcgg
     1921 ctgcgtcaag cagtccgatc gggacgaaaa tgcagaacga aagcaccagg atgtccaccc
     1981 accaggcccg gtagacatga aagatcccgg caaagatctc cccgatctca cgaagcaggt
     2041 tccgcaagcg gctgttctcc ctgactgagg cggcgcgcat cgtgagaagt ctctcaatca
     2101 cgttggcccc gtgcgatcgg accgggtcgg aagggatcag ccgagttcga gggtggtgat
     2161 gccgaagatt cggtctagag gaaggtcggg gggcaggccg cggtacatgc ggatggtggg
     2221 gaagctccgt ttcatcccca ggctggtgaa gagatcggaa gcggcctgat tgggttcggg
     2281 ggcgtcgatc gagaccggac cggtgagcct ggtcgcgagt tcaagcagca gatttccggc
     2341 gatcgttgcg ttggcggcaa agaaggggcc gatccggttg ccggtgcggg tcgggcggga
     2401 ggcgacgaag ccggtgatcg aggtgccgtc ggttgcgaca aggctctggc ggccctctcc
     2461 cttgatccat gcgcgaagga aggccggtct ggggccgaag taatgagcgg cgtcaaaggc
     2521 gaccagctgg tcaaagggaa ccgaggagga aggcaggatg ccggcttccg gttccggatc
     2581 gggaagatcg cgagcctcag ccgagaacct ggcgttgcca taagccggag cgaaaccatc
     2641 ggaggcgtag gtcgcctcct gttccgggac cgcgtcg
//
LOCUS       DAOQMF010000203         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22764_length_2675_cov_4.292366, whole genome shotgun sequence.
ACCESSION   DAOQMF010000203 DAOQMF010000000
VERSION     DAOQMF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22764_length_2675_cov_4.292366"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLV77_04355"
     CDS             complement(<1..391)
                     /locus_tag="PLV77_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNC15109.1"
                     /translation="MDPETAGITGARRFVPEGRLSLGRLASAATNCRGCPLWRGASQV
                     VFGEGSRTAELMLVGEQPGDREDRDGRPFVGPAGLELNRILDETGIERNRLYVTNAVK
                     HFKFHREGKRRIHDKPTRNEVKACRPWL"
     gene            500..751
                     /locus_tag="PLV77_04360"
     CDS             500..751
                     /locus_tag="PLV77_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HNC15110.1"
                     /translation="MGLIAGLIAGLVARKIFEQGWSSLSDEDPPSPDEESLSLGKLVL
                     ALLLEGAVFSLVRGLTDHGARVAFRRYTGAWPGETGKDN"
     gene            complement(761..1096)
                     /locus_tag="PLV77_04365"
     CDS             complement(761..1096)
                     /locus_tag="PLV77_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNC15111.1"
                     /translation="MGIVVYEKPTCTTCRNLFRLLEAEGIDFESVEYHVTGLTEDEIR
                     GLLGKAGITAAEALRMREEGARELAGEDEDTIIAAMAERPELLQRPFVVNGERAVLAR
                     PVERALEVI"
     gene            complement(1106..1606)
                     /locus_tag="PLV77_04370"
     CDS             complement(1106..1606)
                     /locus_tag="PLV77_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNC15112.1"
                     /translation="MDVIDELVENNREFSSRVPDQDLDVKPSRQLTIVTCMDSRIDVY
                     QALGLKDGEAHILRNAGGVITDDVIRSLAISQRRLETRAIMLIHHTNCGMQTITDDGF
                     RSELQEATGTAPAFAIESFTDVEADVAQSIRRVRRSEFIPHRDNVRGFVYNVEDQTLT
                     EVTVED"
     gene            complement(1652..2185)
                     /locus_tag="PLV77_04375"
     CDS             complement(1652..2185)
                     /locus_tag="PLV77_04375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC15113.1"
                     /translation="MDRKLGVLIGLIVLIGALVAVILIGRGSGDSGDSAASGEIPSEV
                     TAGVSEDLSERPKLAGTSETPPTALVKKDIVVGDGAEAKEGDTVKVQYVGALYDNGKE
                     FDASWDRNKEAFSFQLGGGQVIKGWDEGVAGMKVGGRRVLVIPPDLGYGPQGSPPTIP
                     GNSTLVFVVDLEDVQGQ"
     gene            2247..>2675
                     /locus_tag="PLV77_04380"
     CDS             2247..>2675
                     /locus_tag="PLV77_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15114.1"
                     /translation="MKSLLVLPAGPRARWVTVAVWFLAMFVVFGTNIPGKFADAENNE
                     SVSYLPGDAEATRALEAQKKLVGSEEAPMVIIYRREGGLTPADKARIKADVHELNEKV
                     EGVSKRFVITQRSKDGSAVIVTSSITSTGEGEDIREPVEAA"
BASE COUNT          437 a          877 c          876 g          485 t
ORIGIN      
        1 cgagccacgg tcggcaggcc ttgacctcgt tccgggtggg cttgtcgtgg atccggcgct
       61 tgccttcccg gtggaacttg aagtgcttga ccgcgttggt cacgtagagc cggttccgct
      121 cgatcccggt ctcgtcgagg atccggttga gttcgaggcc ggccggtccg acgaacggcc
      181 ggccgtcccg gtcctcgcgg tcaccgggct gctcgccgac cagcatcaac tccgcggtcc
      241 ggctgccttc tccgaacacc acctggctcg caccgcgcca gagcgggcaa ccccggcaat
      301 ttgtggcagc gctcgccaac cggcccagtg acagccggcc ctcgggcacg aaacgccggg
      361 cgccggtgat gccggcggtt tcgggatcca tgacggcggc agtacccgat tcccggtccg
      421 gccaaccgcg ctgcgacggc ggtgtttcga tgctggacga acgggcaccg gtcagtccat
      481 gaccgtactt ttccgcccga tgggtttgat cgccggtttg atcgccggcc tcgttgcacg
      541 caagatcttc gagcagggat ggagttcgct cagcgacgaa gacccgccct cgccggacga
      601 ggaatcactc agcctcggga agctggtcct ggcgctcctg ctggagggcg cggtcttcag
      661 cctggtccgg ggactgaccg atcacggcgc ccgggtcgcg ttccggcgct acaccggagc
      721 ctggccggga gaaaccggca aagacaactg accgcccggg tcagatgacc tcgagcgccc
      781 tttcaacggg gcgggcgagg acagcccttt cgccgttgac gacgaagggg cgctggagga
      841 gctccggccg ttcggccatc gcggcgatga tggtgtcttc atcttcgccg gccagctcgc
      901 gggcaccttc ctcgcgcatg cggagcgcct cggccgcggt gatgccggcc ttgcccagca
      961 ggccgcgaat ctcgtcctcg gtcaggccgg ttacgtggta ctcaaccgac tcgaaatcaa
     1021 tgccctcggc ttccagcagc ctgaagaggt tgcggcaggt ggtgcaggtc ggcttctcgt
     1081 aaacgacgat gcccacggtg tccggctagt cctcgaccgt gacttcggtc agggtctgat
     1141 cttccacgtt gtagacgaag ccacgcacgt tgtcgcggtg ggggatgaac tccgagcggc
     1201 gcacccggcg gatcgactgc gcgacatcgg cctcgacgtc ggtgaacgat tcgattgcga
     1261 aggcgggtgc ggtgccggtc gcttcctgaa gctcggacct gaagccgtcg tcggtgatcg
     1321 tctgcatgcc gcagttggtg tggtggatca gcatgatcgc cctggtctcc agcctgcgct
     1381 gggagatcgc gagggaccgg atcacgtcat cggtgatcac cccgccggcg ttcctgagga
     1441 tgtgggcctc gccgtccttg aggccgagcg cctggtagac gtcgattcgc gaatccatgc
     1501 aggtgacgat cgtcaactgc cggctcggct ttacgtcaag gtcctggtcg ggaacccttg
     1561 aagagaactc ccggttgttc tcgaccagtt cgtcgattac gtccattggt tcaaaccttt
     1621 tgagccggga agccccgatt gaggccgggt gttactggcc ctggacgtct tcgaggtcaa
     1681 ccacgaagac cagggtcgag ttgcccggaa tggtcggagg gctgccctgg gggccgtagc
     1741 cgagatcggg cggaatgacc agcacgcgcc ggccgccgac cttcatgccc gcgacacctt
     1801 catcccagcc cttgatcacc tggccgccgc cgagctggaa gctgaaggct tccttgttgc
     1861 ggtcccagga ggcgtcgaat tccttgccgt tgtcatagag cgcaccgaca tactggacct
     1921 tgaccgtgtc gccttccttc gcctcggcgc cgtcaccgac cacgatgtcc ttcttgacca
     1981 gcgcggtggg gggcgtctcg ctggtgccag ccagcttcgg ccgttcgctc agatcctccg
     2041 aaaccccggc ggtgacttca ctcgggatct cgcccgaagc ggccgaatca cccgagtcgc
     2101 cgctgccgcg gccgatcagg atgacggcga cgagcgcgcc gatgagaacg atcaggccaa
     2161 tgagaacccc aagcttccga tccatgccgc gcatattacg gtgaacccgg ccgggttgcc
     2221 tcgggccggt cgctagggtc tctggcatga agtctctcct cgtcctgccg gccgggcccc
     2281 gggcccggtg ggtaacagtc gctgtctggt tcctcgcgat gttcgtcgtc ttcgggacga
     2341 acattcccgg caagttcgcc gacgccgaaa acaacgagtc ggtgtcatat ctccccggtg
     2401 acgcggaagc cacccgagcc ctggaagcgc aaaagaagct ggtcggctcg gaggaagcgc
     2461 cgatggtgat catctaccgg cgcgaagggg gcctcacccc ggccgacaag gcccggatca
     2521 aggccgatgt tcatgaactc aacgagaagg tcgagggagt cagcaagagg ttcgtcatca
     2581 cccagcgatc gaaggacggc tcggcggtca tcgtcacctc ctcgatcact tccaccggag
     2641 agggagaaga catccgcgaa ccggtcgagg ccgcc
//
LOCUS       DAOQMF010000204         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22766_length_2675_cov_3.532061, whole genome shotgun sequence.
ACCESSION   DAOQMF010000204 DAOQMF010000000
VERSION     DAOQMF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22766_length_2675_cov_3.532061"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLV77_04385"
     CDS             complement(<1..449)
                     /locus_tag="PLV77_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNC15115.1"
                     /translation="MTKGLVVTYLSLIVLIPLAAIAAKAFSAGPAEFWDAIRQPQAIA
                     ALKLTAICSLIVVAINAVMGTLIAWVLVRDEFPGKSIVNSMIDLPFALPTIVASLTLL
                     TLYGNDSFLGIHAAYTKFAVIIALLFVTLPFVVRAVQPLLLSLDRDM"
     gene            complement(514..1536)
                     /locus_tag="PLV77_04390"
     CDS             complement(514..1536)
                     /locus_tag="PLV77_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC15116.1"
                     /translation="MSFSSSKKTRRSIQALLVLALVAFGAIIAGCGSSDGDSGSKVQL
                     VAYSTPQTAYEEGLEPAFNETPEGDGVEFSNSFGASGDQSRAVEAGQPAGYVNFALET
                     DVTRLVDAGLVSKDWQNNKYKGIVTNSVVSIVTRPGNPEGIESFQDIIDKDVSVVTPN
                     PFTSGGARWNIMAIYGAALHEGKSEQEALDTVKQVLSQAEVQPASARDALQAFVGGAG
                     DVLISYENEAIAAQNAGEDVDYVIPDSTILIEQPAAVTVDAPKSATDFLNYLWSDAGQ
                     KIWAEQGYRPVNKSLVDESVYPTPKDEFTIAEFGGWDKVADEFFDDKTGSIAEIEKEL
                     GVSTEG"
     gene            1757..>2675
                     /gene="xseA"
                     /locus_tag="PLV77_04395"
     CDS             1757..>2675
                     /gene="xseA"
                     /locus_tag="PLV77_04395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNC15117.1"
                     /translation="MSEELPEPGNPAAAEDAAPTPVGDYARQLKQDLKRLPEATILGE
                     ITNFSQSRVQIYFELRDERGGVPGTMWKREFDALGLPAGTVKVGSQVVVTGAPDYYEG
                     GKNASPSFSFRAIDIRPFGEGDLIARLAALRKQFQAEGLTEPQKNLPRRLLPRRIGVI
                     TAEGGAARQDLMVGLERRGWKGEVVWGFAPVQDRKAAPQITRILSDMAAFADVDSIVV
                     CRGGGSLTDLWAFCDEDLCRTVVMLAVPVISAVGHERDVTLLDDVAAVRASTPTHAAE
                     AVVGIDIPAARASLARAAGTTRRVSQAAVR"
BASE COUNT          462 a          818 c          912 g          483 t
ORIGIN      
        1 tccatgtcac ggtcaaggga cagcagcagc ggctggacgg cccgcaccac gaagggaagg
       61 gtcacgaaga gcagggcgat gatcaccgcg aactttgtgt aggcggcgtg aatcccgagg
      121 aagctgtcat tgccgtagag cgtgagcagg gtgaggctgg cgacgatggt cggcagggcg
      181 aagggcaggt cgatcatcga gttgacgatg ctcttgccgg ggaactcgtc ccggaccagg
      241 acccaggcga tcagggtgcc catcaccgcg ttgatcgcca cgacgatcag ggagcagatc
      301 gcggtcagct tcagggcggc gatcgcctgc ggctgcctga tcgcgtccca gaactcggcc
      361 ggacccgccg agaaagcctt ggccgcaatc gccgccagcg ggatcagcac gatcaggctc
      421 aggtaggtga cgaccaggcc cttggtcaag gcggtttcaa ccccgggcag gcgcaggccc
      481 gcccggggtc gaggtgccgc tacggctgtg gattcagcct tcagtggaaa ctcccagttc
      541 cttctcgatc tcggcgatcg agccggtctt gtcgtcgaag aactcgtcgg cgaccttgtc
      601 ccagccaccg aactcggcga tcgtgaactc gtccttcggg gtcgggtaga cggactcgtc
      661 aaccaggctc ttgttgaccg ggcggtaacc ctgctcggcc cagatcttct ggccggcatc
      721 gctccagaga tagttgagga agtcggtcgc ggacttcggg gcatcgaccg tgactgcggc
      781 cggctgctcg atcaggatcg tcgaatccgg aatcacatag tccacgtcct cgccggcgtt
      841 ctgcgcggcg atcgcctcgt tctcgtagga gatcaggacg tcaccggcgc cgcctacgaa
      901 agcctggagg gcgtcacggg cgctggccgg ctggacttcg gcctgcgaga ggacctgctt
      961 gacggtgtca agggcttcct gctcgctctt gccctcatgc agggcggcac cgtagatggc
     1021 catgatgttc cagcgggcgc caccggaggt gaacgggttc ggggtcacga cacttacgtc
     1081 cttgtcgatg atgtcctgga aggactcgat gccttccggg ttgccgggac gggtcacgat
     1141 cgagacgacc gagttggtca cgatgccctt gtacttgttg ttctgccagt ccttcgagac
     1201 gaggccggca tcaaccagac gggtcacgtc ggtctcgagc gcgaagttga cgtacccggc
     1261 gggctggccg gcttcgacgg ctcggctctg gtcgccggaa gctccgaacg agttcgagaa
     1321 ttcgacaccg tcaccttctg gtgtctcgtt gaaagccggc tcgagacctt cttcgtaggc
     1381 ggtctggggg gtggagtagg cgaccagctg gaccttgctg ccactgtcgc cgtcgctgct
     1441 gccgcaaccg gcgatgatcg cgccgaaggc tacgagagcc aggacgagaa gcgcctgaat
     1501 gctgcgccgg gtcttcttgg aactgctgaa actcattcgg gattcccccc ttgttgatgt
     1561 ggttactttc tttacctaca aactcaaata acgacgggca aggtagcacg ggtccggaga
     1621 gaatgtcgag tcaactggtc gtaatagtca tatttaccgt cggcatcttc gggttttccc
     1681 ctgtttcaag ccaaaagcac gatcctgtgc cgatccgtca cagaggcccg tcggcggtcg
     1741 gtgtcatgct ggagcgatga gcgaagagct tccggaaccg ggcaaccctg cggcggcaga
     1801 ggatgccgcg ccgaccccgg tcggcgacta tgcccgccag ctgaagcagg acctgaagcg
     1861 cctccccgaa gcgacgatcc tcggcgaaat cacgaacttc agccagtcac gggtccagat
     1921 ctatttcgag ctgcgcgacg aacgcggcgg ggtgccggga acgatgtgga agcgggagtt
     1981 cgacgccctc ggcctgccag cgggaaccgt caaggtcggt tcgcaggtag tcgtgaccgg
     2041 cgcgcccgat tattacgagg gaggcaagaa cgcttcgcct tccttcagtt tccgggccat
     2101 cgacatccgt ccgttcgggg agggggacct gatcgcccgt cttgcggcgc ttcgcaaaca
     2161 gttccaggcg gaagggctga ccgagccgca gaagaacctg ccgcgcaggt tgctgccccg
     2221 gcggatcggg gtgatcaccg cggaaggtgg ggcagcccgg caggatctga tggtcggcct
     2281 cgagcgtcgc ggctggaagg gcgaggtggt ctggggcttt gccccggtcc aggaccgcaa
     2341 ggcggctccg cagatcacga ggatcctcag tgacatggcc gctttcgcgg atgtggactc
     2401 gatcgtcgtc tgccggggcg gcggcagcct gactgacctc tgggccttct gcgacgagga
     2461 tctctgccgc acggtcgtga tgctcgcggt gccggtcatt tccgcggtgg gtcacgaacg
     2521 ggacgtgacc ctgctcgacg acgtggcggc ggttcgggcc tcaaccccga cccatgcggc
     2581 cgaagccgtg gtcgggatcg acatcccggc cgcccgcgca agcctggccc gggcggcggg
     2641 taccactcgc cgggtcagcc aggcagcggt gcgcg
//
LOCUS       DAOQMF010000205         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_22844_length_2670_cov_4.175143, whole genome shotgun sequence.
ACCESSION   DAOQMF010000205 DAOQMF010000000
VERSION     DAOQMF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22844_length_2670_cov_4.175143"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLV77_04400"
     CDS             <1..602
                     /locus_tag="PLV77_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15118.1"
                     /translation="TVPLLAKLLKGSKAPSTQARQMLNQMVDWNKKNGSRLDRDGDNL
                     IDAPGAASLDAAWPKIADAFMKPVIGSQLDELSSLFSRFDQPPSGQYSGWYQYFDKDI
                     RALLGQKVKSPFKVKYCGHGKKAACQKAVWNAIAAAGTEAAAQYGSENPAQWHVNTAY
                     VDDDHRGEQIKFSPLTLVTMRYTNRPSGIQQVISFNGHR"
     gene            882..2318
                     /gene="pyk"
                     /locus_tag="PLV77_04405"
     CDS             882..2318
                     /gene="pyk"
                     /locus_tag="PLV77_04405"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNC15119.1"
                     /translation="MNQIHSETRRTKIVATVGPASWDPSVLEQMIIAGVDVFRLNFSH
                     GDEATHVRVIKDVRLISERLDKEVGILGDLPGPKLRVGEYPQGVVDIIHGSTVTITPD
                     DVPGTETLIPVDWPELAQALSVEDTVYLADGSIRLRVVANDGRTVECEVEVGGPLSSH
                     KGMNLPGVEAGLDSVSEDDLRWVEFAVANQLDYIAVSFVRKAEDLDPVLNKLKELRSD
                     IPIIAKIEKPQAADAVEEIVDRATGGIMVARGDLGIEVPLSKVPVLQKSLIGAAGRAS
                     KTVITATQMLASMVSAKRPTRAEVTDVATAIYDGTDAIMLSEETAVGEFPVEAVRVMD
                     QIARGTETDLPYWDWVLNRVPQDDQDVSDSVTQSAVIAAYRLNLQAIVVPTASGRTAK
                     VVAAHRPQVPVLAVTHSVRTQRQLKLIFGVRPVVNDQTTEIRNLLYECADDAVRFGAA
                     ASGDLIAIVAGLSDMQLGTNLFEVHRVP"
     gene            2403..>2670
                     /locus_tag="PLV77_04410"
     CDS             2403..>2670
                     /locus_tag="PLV77_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC15120.1"
                     /translation="MSVVEIRNPEGTELLGEVEPTDPAEVGGVVERALAARPAWSALP
                     VAERAAMLDRAADLLEPEQETLGRLLAQESGKPIGQAEFEVGGRI"
BASE COUNT          491 a          864 c          882 g          433 t
ORIGIN      
        1 tcaccgtgcc gctgctggcc aagctgctga agggctcgaa ggcccccagt acccaggccc
       61 ggcagatgct caaccagatg gtcgattgga acaagaagaa cggcagccgt ctggaccgtg
      121 acggcgacaa cctgatcgac gccccgggcg ccgcctcgct ggacgccgcc tggccgaaga
      181 tcgccgacgc gttcatgaag ccggtaatcg ggtcgcagct cgacgagctg agctcgctgt
      241 tctcacggtt cgatcagccg ccgagtggcc agtactccgg ctggtaccag tacttcgaca
      301 aggacatccg ggcgctgctc ggccagaagg tcaagagtcc gttcaaggtc aagtactgcg
      361 gccacggcaa gaaggccgcc tgccagaagg cggtctggaa tgcgatcgcc gccgccggca
      421 ccgaagcggc cgcccagtac ggctcagaga acccggccca gtggcacgtg aacacggcct
      481 acgtggatga cgaccatcga ggcgagcaga tcaagttcag cccgctcacc ctggtcacca
      541 tgcgctacac caacaggccg tccggcatcc agcaggtgat ctccttcaac ggtcaccgct
      601 aggtccggcc aaagcccgat cgaggagaaa gcccgcccca tccctggggc gggctttcct
      661 ctttccatgg cggtgcaggc actcaccgcc aggcctatcc atcgggtcgt agggggatcg
      721 ccccggtgga atgaaggccg taatttcggt ttaccccgga ttccgaactg aaaaccgaag
      781 tccgccgttc aactgcggat aggttgatca gtccgcgcga gcgggacccc cgtttacagg
      841 cgtttacagg ccggacccgc cggactgaga gtattccggg ggtgaaccag atccactccg
      901 aaacccgtcg tacgaagatc gtcgccacgg tcggtcccgc ctcatgggat ccgtccgtgc
      961 tcgagcagat gatcattgcc ggggtagacg tcttccggct caacttttct cacggtgacg
     1021 aggcgactca cgtacgggta atcaaggacg ttcgcctgat ctcggaacgg ctcgacaagg
     1081 aagtcgggat cctcggggac ctgccggggc caaagctccg ggtcggtgaa tacccgcagg
     1141 gagtggtgga catcatccac ggttcgaccg tgacgatcac gcccgatgac gtgcctggaa
     1201 ccgaaaccct gatcccggtc gactggcccg aactggccca ggccctgagt gtcgaggaca
     1261 cggtctatct ggccgacggc tcgatccgcc tgcgcgtggt tgccaacgac gggcggacgg
     1321 ttgagtgcga ggtagaagtc ggcgggccgc tctcgtcgca caagggaatg aacctgcccg
     1381 gcgtcgaagc cgggctcgac tcggtttccg aggacgacct gcgctgggtc gagttcgcgg
     1441 tcgccaacca gctcgactac atcgcggttt ccttcgtccg caaggcggag gatctcgacc
     1501 ccgtgctgaa caagctgaag gagctccgct cggacatccc gatcatcgcc aagatcgaga
     1561 agccgcaggc cgccgacgcg gtcgaggaaa tcgttgaccg ggccaccggc gggatcatgg
     1621 ttgcccgcgg cgacctcggc atcgaggtgc cgctttccaa ggttcccgtg ctgcagaaga
     1681 gcctgatcgg ggcagccggc cgggcttcca agacggtgat caccgcgacc cagatgctgg
     1741 cctcgatggt ttcggccaaa cgcccgacca gggccgaggt gaccgacgtc gcgaccgcga
     1801 tctatgacgg caccgacgcg atcatgctct cggaagagac cgcggtcggc gagtttccgg
     1861 tcgaggcggt cagggtcatg gaccagatcg cccgcggcac cgaaacggac ctgccgtact
     1921 gggactgggt cctcaaccgg gttccgcagg atgatcagga tgtctccgac tcggtcaccc
     1981 agtcggcggt gatcgccgcc taccggctga acctgcaggc gatcgtggtg ccgaccgctt
     2041 ccggtcgcac cgccaaggtc gtggcggccc accgcccgca ggttccggtg ctggcggtga
     2101 cccacagcgt gcgcacccag cggcaactca agctgatctt cggcgtgcgg ccggtcgtca
     2161 acgaccagac caccgagatc cgcaacctgc tctacgaatg cgccgacgac gcggtccggt
     2221 tcggggcggc ggcgtcgggt gacctgatcg cgatcgtggc cggactatcc gacatgcagc
     2281 tgggtaccaa cctgttcgag gtgcaccgcg taccctgaaa ttcggaactt tgaacctggt
     2341 tctgtaggct gaactttgat actgacgtct agttgggtac ctgcgtacct cgaggtggga
     2401 atttgagcgt tgttgagatc aggaatccgg aagggaccga gctgctgggc gaggtcgagc
     2461 cgaccgaccc ggcggaggtc ggcggagtgg tcgaacgggc cctcgccgcc cggcccgcct
     2521 ggtccgccct gccggtggcc gaacgggccg cgatgctgga ccgggccgcg gacctcctcg
     2581 agccggagca ggaaacgctg ggccggttgc tcgcgcagga gtcgggcaag ccgatcggcc
     2641 aggccgagtt cgaggtgggc ggacgcatcc
//
LOCUS       DAOQMF010000206         2647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_23230_length_2647_cov_3.547454, whole genome shotgun sequence.
ACCESSION   DAOQMF010000206 DAOQMF010000000
VERSION     DAOQMF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23230_length_2647_cov_3.547454"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..847)
                     /locus_tag="PLV77_04415"
     CDS             complement(77..847)
                     /locus_tag="PLV77_04415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC15121.1"
                     /translation="MSAQDKPPFPPLDLADRVSSLPETDNALDHFEWLGLMTRQSIDA
                     ALPEDWNYEGKRLLDFGCGSGRTLRHFLDVAEVAEAWGCDIDRPSIEWLEDNLCPPLR
                     VFTCEVDPPLKSDRPGLEAGSFDLIWAVSVFTHLAGNSAEWLLELHRLLKPGGILIAS
                     FMGRLTHQPLTGEPWDERGTGMKAIGLDNPWEKGGPSVFMSDWWVREHWGRAFEVEII
                     DAGIETLNQSWAVMKKKSVELTADGLLKEKPRRWWRRG"
     gene            complement(870..1829)
                     /locus_tag="PLV77_04420"
     CDS             complement(870..1829)
                     /locus_tag="PLV77_04420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC15122.1"
                     /translation="MAPGKRVTDPLQWGYSLSNVFELLSGCLEAIETKTVLEIGSYKG
                     ELTVELLEWAQTRGVKVAGVDPLPPDELEALAKAHPELELLRQTGQEVLREREFDDAI
                     IIDGDHNYYTLSEELKIIGERAPGAGMPLLMFHDVCWPHARRDTYYEPDRIPEDQRQP
                     LVHNEGIAPGIKGTAEWGLPFVWAAAEEGGEKNGTMTAIEDFLAERPGIQLAVVPAFF
                     GFGVMWHRDAPWASAVERVIAPYDRHPVLERMEGNRVEHLVAGQARARQLVKLEEKVA
                     KQEDLLRRLLGSSAFTAAEKLSGLKQRGKPLFTREEVQSLLDE"
     gene            1860..2459
                     /locus_tag="PLV77_04425"
     CDS             1860..2459
                     /locus_tag="PLV77_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15123.1"
                     /translation="MDDLGQHNDAASLMADLRSRHPRFTEAVLADTVVTAQHRGERYE
                     FRGKGDALVAALRLAWVSDAFLAQVLYRAKAACQRREVRVLPRLFHRLSMMLAQVSIG
                     DPVVMHPGVYIVHGQVVLDGIVEIHPGVVISPWVTIGLRAGNVVGPTVREQVNIGTGA
                     KLIGPIDIGAASSIGANAVVVDDVPAGTTAVGVPASQPG"
BASE COUNT          419 a          888 c          876 g          464 t
ORIGIN      
        1 gcacctcgtg gaagatcacc cgccccctcc gccgcctccg cggcagccgc gaaaaaggca
       61 agtagccggc ggcgattcag ccgcggcgcc accagcgtcg gggtttctcc ttcagcagcc
      121 cgtcagcggt cagctcgacc gacttctttt tcatcacggc ccagctctgg ttgagggtct
      181 cgatgccggc gtcaatgatc tcgacctcga aggcgcggcc ccagtgctcg cggacccacc
      241 agtcggacat gaagaccgag gggccgccct tctcccaggg attgtcgaga ccgatcgcct
      301 tcatccccgt accccgctcg tcccagggtt cgccggtcag cggctgatgg gtcaggcggc
      361 ccatgaagct ggcgatcagg atgccgcccg gtttcagcag ccggtgaagt tcaagcagcc
      421 actcggccga gtttccggcc aggtgggtga agaccgagac cgcccagatc aggtcgaagc
      481 tgcccgcctc cagcccgggc cggtcggact tgagcggcgg gtccacctca caggtgaaga
      541 cccgcagggg cgggcagagg ttgtcttcca gccactcgat cgagggtcgg tcgatgtcgc
      601 atccccaggc ctcggccacc tcggccacat caaggaagtg ccggagcgtg cgacccgacc
      661 cgcagccgaa atcgagcagc cgtttgccct cgtagttcca gtcttccggc aaggcggcgt
      721 cgatcgactg tcgggtcatc aggcccagcc actcgaagtg gtcgagcgcg ttatcggtct
      781 ccggcaggga gctgacccgg tcagccaggt ccaggggcgg aaacggcggc ttgtcctgcg
      841 cgctcaccga aagctccgcg ggcagatgcc tactcgtcga gcagcgactg gacttcctcg
      901 cgggtgaaga gcggcttgcc gcgctgcttc agcccggaga gcttctcggc cgcggtgaag
      961 gcgctcgatc cgagcagccg ccgcagcaga tcctcctgtt tggcgacctt ctcttccagc
     1021 ttgaccagct ggcgggcccg ggcctggccg gcgaccaggt gctcgacccg gttgccttcc
     1081 atccgttcga ggaccgggtg gcggtcatag ggggcgatca cgcgctcgac cgcgctagcc
     1141 cagggagcat cccggtgcca catcacgccg aagccgaaga aggccgggac taccgccagc
     1201 tggatgccgg gccgttcggc caggaagtcc tcgatcgcgg tcatcgtgcc gttcttctcc
     1261 ccgccttcct cggcggcggc ccagacgaag ggcaggcccc attcggcggt gcccttgatc
     1321 ccgggggcga tgccctcgtt gtgaaccagt ggctggcgct gatcctcggg gatccggtcc
     1381 ggctcgtagt aggtgtcgcg ccgggcgtga ggccagcaga catcgtggaa catcagcagc
     1441 ggcatcccgg cgccgggagc gcgctcgccg atgatcttca gttcctcgct cagggtgtag
     1501 tagttgtggt cgccgtcgat gatgatcgcg tcatcgaatt cccgttcgcg cagcacctcc
     1561 tggccggtct ggcgcaggag ttcaagctcc gggtgcgcct tcgccagggc ttcgagctcg
     1621 tccggcggca gcgggtcgac tcccgcgacc ttgaccccgc gtgtctgcgc ccattcgagc
     1681 agctcgaccg tcaactcgcc cttgtaggag ccgatttcga gcacggtttt cgtttcgatc
     1741 gcttccaggc agcccgaaag cagctcgaag acgttgctca gcgagtagcc ccactggagc
     1801 ggatcggtaa cgcgttttcc gggagccatc ggcggcaagg gtatagattc gcccggccca
     1861 tggatgacct cggtcagcac aatgacgccg ctagcctgat ggcggacctg cgctcccggc
     1921 acccccggtt caccgaagcg gtgctggccg acacggtggt gaccgcccag caccgggggg
     1981 agcgatacga gttccgcggc aagggggatg ccctggtcgc ggcccttcgc ctggcctggg
     2041 tgagcgacgc cttcctggcc caggttctct accgcgccaa ggccgcctgc cagcggcgcg
     2101 aagtccgggt tttgccccgg ctctttcacc ggctttcgat gatgctggcc caggtttcga
     2161 tcggcgaccc ggtggtgatg caccccggcg tctacatcgt ccacggccag gtcgtgctcg
     2221 acggcatcgt cgaaattcat cccggcgtgg tgatttcacc ctgggtcacg atcggcctgc
     2281 gggccggcaa cgtggtcggt cccacagttc gcgagcaggt gaacatcggt acgggcgcca
     2341 agctgatcgg accaatcgac atcggggctg cctcatcgat cggagccaac gcggtcgtgg
     2401 tcgacgacgt cccggccggg accaccgcgg tgggagttcc ggccagtcaa cccggttgaa
     2461 accgggcatg cgggtaagta ctgaacctgt tctgctttgc aggccgggct ggtagcgtcc
     2521 agcgggttcg agaaagcaga accgaactca ccagctaaag gcaggttttt gatgggcatt
     2581 tttcgtcgca aggatcgtgg agcagccgca gtcgccgagg ccgagccgga actcaatccg
     2641 gtggtcg
//
LOCUS       DAOQMF010000207         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_23340_length_2640_cov_3.549323, whole genome shotgun sequence.
ACCESSION   DAOQMF010000207 DAOQMF010000000
VERSION     DAOQMF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23340_length_2640_cov_3.549323"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLV77_04430"
     CDS             <1..217
                     /locus_tag="PLV77_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15124.1"
                     /translation="TDIHALGLVLILSGIFTVQKGDPVVWPIAAVVAGMGFALILMAP
                     RWYQHFRPPEAIAAEQRAAGKQPPEAD"
     gene            341..796
                     /locus_tag="PLV77_04435"
     CDS             341..796
                     /locus_tag="PLV77_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC15125.1"
                     /translation="MRVSAKADYAVRAVAELAASPDGPVKGERLADSQDIPLQFLEHI
                     LLDLKHSGIVRARRGAKGGYWLAKPAEDVSIADVIRAVEGPIAHVQSAPPESITYTGS
                     ARELQNVWIAVRASLRDVLESTSLADLVSGNLPDNVQRLADDKDAWEPR"
     gene            925..2541
                     /locus_tag="PLV77_04440"
     CDS             925..2541
                     /locus_tag="PLV77_04440"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNC15126.1"
                     /translation="MTEGLTRRDALRAGAGAAGGMALAGGLLGRAIDAMGAPAVIGDG
                     PYGPLGTPDANGLRLPAGFTSRKIARSGENIGPRPYQFHILPDGMGSFKTSDGGFILV
                     SNSEAPYLPGLAEIGAGAIRFNKDFEVVDAYPILKETWVNCAGGVTPWGTWLSCEEIE
                     TGKVFECDPKGRKPFDGSNWIERPALGVFKHEACCVDPAGKQVYLTEDLGDGCLYRFT
                     PDRYPDLSSGRLDVASVANDGTVTWHAVPDPQYAGSVPTRHQVEAATHFRRGEGMWFD
                     AGKVYFSTTQDDRVYAYHPASAHLEVLYDGVALGDEAPLHDTDNVTVSPISGDLFVCE
                     DPGGNAVNGLQVCIISAEGEAAAFCEAPASGHKDSELTGPVFDPTGSRLYFSSQRAKF
                     PGETVSAGIIYEISGPFRKLREDEKPVDHKKPLVKVRTLGMPSLAGLVKNGQAFQIDV
                     HDQSFPVDVEARLIAPLRRRQGRGTREVTLGTLKLTLHRPGAKKIRLKIKEGYRKRIR
                     QRRISGARLELIARDAAGNQRKVIKQVSFR"
BASE COUNT          456 a          896 c          845 g          443 t
ORIGIN      
        1 gacggacatc cacgccctcg gcctggtgct gattctctcc ggcatcttca ccgttcagaa
       61 gggcgacccc gtggtctggc cgatcgccgc ggtggtggcc ggcatgggct tcgcgctgat
      121 cctgatggca ccgcggtggt accagcattt ccggcccccg gaagcgatcg ccgcggaaca
      181 gcgggcagcc gggaaacagc ccccggaagc cgattagcac tgccttccgc gcctcgggaa
      241 gcgaaattcc cgattctgct gaccgatttg atcgcgaaat accgtgaccg ggcccggcag
      301 cctgcggacc cgcgccggtt cgggctgatt tactaggccc atgcgcgtct ccgccaaggc
      361 cgattacgcc gtcagggcag ttgccgaact cgccgcgtct ccggacgggc cggtcaaggg
      421 tgaacgactc gccgattccc aggacatccc gcttcagttt ctcgagcaca tcctgctcga
      481 cctgaagcac tcggggatcg tccgcgcccg tcgcggcgcc aagggtggct actggctggc
      541 caagccggcc gaagacgttt cgatcgccga cgtgatccgc gcggtcgaag gtccgatcgc
      601 ccacgtccag tcggctccgc ccgagtcgat cacctacacc ggcagcgcca gggaactcca
      661 gaatgtctgg attgctgtcc gcgccagcct ccgtgacgtg ctggaatcaa ccagcctcgc
      721 cgacctcgtc tccgggaacc tcccggacaa cgttcagcgg ctggccgatg acaaggacgc
      781 ctgggaaccc aggtaggtgc cccgggcggc gcgagggacc accgcccgcc catgacttgt
      841 gttgccctgg ccttgccctg accggtagcc tgctttcagg gagagttttt ctacgatttc
      901 cagggagtgg gtatgtcaga ggaagtgact gaaggactga ctcgaagaga cgccttgcgg
      961 gccggggctg gcgccgccgg gggaatggct ctggccggcg gtctgctggg ccgggcgatc
     1021 gacgcgatgg gcgctccggc ggtgattggc gacggcccct acggtccgct cgggaccccg
     1081 gacgccaacg gcctccggct ccccgccggc ttcacttccc ggaagatcgc ccgctccggt
     1141 gagaacatcg ggccccgccc gtaccagttt cacatcctgc ccgacggcat gggctccttc
     1201 aagaccagcg acggcggctt catcctcgtt tcgaacagcg aggcccccta cctgccgggc
     1261 ctggccgaaa tcggagccgg agcgatccgc ttcaacaagg atttcgaagt ggtcgacgcc
     1321 tacccgatcc tcaaggaaac ctgggtcaac tgcgcgggcg gcgtgacccc gtggggaacc
     1381 tggctttcct gcgaggagat cgagaccggc aaggttttcg aatgtgaccc caaaggccgg
     1441 aaaccgttcg acggcagcaa ctggatcgaa cgcccggcgc tcggcgtgtt caagcatgag
     1501 gcctgctgcg tcgatccggc cggcaagcag gtttacctga ccgaggatct cggggacggt
     1561 tgtctttacc gcttcactcc cgaccggtac ccggaccttt cctccggccg gctcgacgtc
     1621 gcttcggtcg ctaacgacgg aaccgtcacc tggcatgcgg tcccggaccc ccagtacgcc
     1681 ggctcggttc ccacacgtca tcaggtcgag gcagcgactc atttccggcg gggcgaaggg
     1741 atgtggtttg acgccgggaa ggtctatttc tcgaccaccc aggacgaccg cgtttatgcc
     1801 tatcacccgg cttcggccca cctcgaggtg ctctatgacg gggtcgccct cggcgacgaa
     1861 gctcccctcc atgacaccga caacgtgacc gtctcaccga tctccggcga ccttttcgtc
     1921 tgcgaggacc cgggcggcaa tgccgtcaac ggcctgcagg tctgcatcat ctcggccgag
     1981 ggcgaggcgg ccgccttctg cgaggcgccg gccagcggcc acaaggattc ggagttgacc
     2041 gggccggtat tcgacccgac cggaagccgg ctctacttca gttcacaacg ggcgaagttc
     2101 cccggtgaaa ccgtgtccgc cggaatcatc tacgagatca gcggcccctt ccggaaactg
     2161 cgggaggacg aaaagccggt cgatcacaag aagccgctgg tcaaggtgcg gacgctgggg
     2221 atgccaagcc tcgccgggct cgtcaagaac ggccaggcct tccagatcga tgttcacgac
     2281 cagagcttcc cggtcgatgt cgaggcccgg ctcatcgcgc ccttgcgccg ccggcagggc
     2341 cgcggcacac gcgaagtgac gctcggaacc ctgaagctca cccttcaccg tccgggcgcc
     2401 aaaaagattc gcctgaagat caaggagggc taccgcaaga ggatcaggca gcggcggatc
     2461 tccggggcca gactggagct gatcgccaga gacgccgctg gcaatcagcg aaaagtgatc
     2521 aagcaggtca gtttccgttg atcccggcgg cccgcttcgg ggcggccgtt ttgctcctcg
     2581 ccagccttgt cgcgggctgt ggttcatcca cggaccagga agggggcgga aacgccgccg
//
LOCUS       DAOQMF010000208         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_23344_length_2640_cov_3.232108, whole genome shotgun sequence.
ACCESSION   DAOQMF010000208 DAOQMF010000000
VERSION     DAOQMF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23344_length_2640_cov_3.232108"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLV77_04445"
     CDS             <1..632
                     /locus_tag="PLV77_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNC15127.1"
                     /translation="IVPLLLGIAGGVMAVASVMGLIIPALDRGTVAEVVGGLAIGTGF
                     LLLARRYLSHVPRNFSRSGGRTGLLVFLVLLVHSLPEGFAVGTAYEADPAQLGIFVVI
                     AIAIQNIPEGTSVAIPLAAAGASGRRQFWMAVASSVPQPIGAVIAYLLVQEISALLPV
                     SFGFAAGAMLALTLLEILPEAWIENPRQTFLGLATSIPLMVALSLALGV"
     gene            complement(689..1144)
                     /locus_tag="PLV77_04450"
     CDS             complement(689..1144)
                     /locus_tag="PLV77_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15128.1"
                     /translation="MKVGKIGVCSLLLMVSLGVTACGSDDSSSDDSSESITKAEFVAQ
                     ANEICAESNKTIDAAEKEAFSGGQPTQADVDSFINDTVLPQVESQINDIEALPVPEGE
                     EDQISAILDAANKALEEGKSDPSSLQGNGDPFAEANKLANAYGMTECGG"
     gene            1197..1910
                     /locus_tag="PLV77_04455"
     CDS             1197..1910
                     /locus_tag="PLV77_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNC15129.1"
                     /translation="MCDGSEMNRDDERIIEGMRRQSEGISKSGAGGAANLERLGWKAG
                     FGTGATMALIGTSQPLAGFLTKATLLDSGAFVQVGGWGVPLFEAEVAVRIDSEIGAGA
                     TAGEATAAAGAVAAAIELVDFGSIESVEEILAGNIFHRHVLLGEFVELPPDGLDGVSI
                     SVAANGEAAEASDPREALGDLGQVVAALADQAALTGESIKPGDVVITGAAIPPAPVSP
                     GDDYGVEITGGSSVTVKLV"
     gene            complement(2005..2078)
                     /locus_tag="PLV77_04460"
     tRNA            complement(2005..2078)
                     /locus_tag="PLV77_04460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2042..2044),aa:Pro,seq:ggg)
     gene            complement(2151..2221)
                     /locus_tag="PLV77_04465"
     tRNA            complement(2151..2221)
                     /locus_tag="PLV77_04465"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2187..2189),aa:Gly,seq:tcc)
     gene            complement(2404..>2640)
                     /locus_tag="PLV77_04470"
     CDS             complement(2404..>2640)
                     /locus_tag="PLV77_04470"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15130.1"
                     /translation="QQPAIALRPELDLLLEAGRDYGALGAMVSGSGPTCVFLARDNEH
                     GLELAVGLSTTGLCRSARIASGPVPGARTTTIIP"
BASE COUNT          437 a          848 c          883 g          472 t
ORIGIN      
        1 ccatcgtgcc cctgctgctc ggcattgccg gaggggtaat ggccgtggct tccgtaatgg
       61 ggctgatcat tccggctctc gaccgcggca ccgtagccga ggtcgtcggg ggcctggcga
      121 tcgggaccgg tttcctgctg ctggcccgcc ggtacctgag ccacgtgccg aggaacttca
      181 gccggtccgg cgggcgcacc gggctgctgg tcttcctggt actgctggtt cacagcctgc
      241 cggaggggtt cgcggtcggc accgcctacg aggccgaccc cgcccagctc gggatcttcg
      301 tggtgatcgc gatcgcgatc cagaacattc ccgagggaac cagcgtcgcg atcccgcttg
      361 ccgccgcggg tgcctcgggc cgccgccagt tctggatggc ggtcgcgagc agcgtgccgc
      421 agccgatcgg tgcggtgatc gcctacctgc tggtccagga gatttccgcc ctgctgccgg
      481 tctctttcgg cttcgctgcc ggggcgatgc tcgccctgac cctgctcgag atcctccccg
      541 aggcctggat cgaaaacccc cgccagacct ttctcggcct ggccacctcg atccctttga
      601 tggtcgccct gagcctggcg ctcggggtct gagcgaccgg ctgcgccttc gcctgacgag
      661 gccggccgct ctcgcaccta tcggatggtc agcctccgca ttcggtcatg ccgtatgcgt
      721 tggcgagttt gttcgcttcc gcgaacggat ccccgttgcc ttgcagggag gaaggatctg
      781 acttgccctc ctcgagagcc ttgttggccg catcgaggat cgcgctgatc tggtcctctt
      841 cgccctccgg aaccggaagc gcctcgatgt cgttgatctg ggactccacc tgaggcagca
      901 cggtgtcatt gatgaacgag tcgacatccg cctgggtcgg ctggccgccg gagaacgctt
      961 ccttctcggc cgcgtcgatc gtcttgttgc tctcggcgca gatctcgttc gcctgggcga
     1021 cgaactccgc cttggtgatc gattccgaag aatcgtcgga cgagctgtca tcggacccgc
     1081 aggctgtcac gcccagcgag accataagga gcaacgaaca gacgccgatc ttcccaacct
     1141 tcaatgcact catactcccc cttcggttga ctggtctcag ttgaaactaa ccgcaaatgt
     1201 gcgacgggag tgaaatgaac cgggatgacg agcgaatcat cgaggggatg cggcgacagt
     1261 ccgaaggcat ttcaaagtcg ggtgcgggtg gggccgcgaa cctggagcgg ctcggctgga
     1321 aggccgggtt cggcaccggg gcgacgatgg cactgatcgg gaccagccag ccactggcag
     1381 ggttcctgac gaaggcgacg ctgctcgact cgggggcatt cgtccaggtc ggcggatggg
     1441 gcgtgccttt gttcgaggcc gaggtcgcgg tccggatcga ttcggaaatc ggtgccggcg
     1501 cgactgccgg agaggcgacg gccgcggccg gtgcggtggc ggccgcgatc gagctggtcg
     1561 acttcggatc gatcgagagc gtcgaggaga tcctcgccgg gaacatcttc catcgtcatg
     1621 tgttgcttgg tgagttcgtc gagctccctc cggacgggct cgacggggtc agcatttcgg
     1681 tggccgccaa cggagaggcg gcggaggcct ccgacccccg ggaggccctc ggtgatctcg
     1741 ggcaggtggt ggccgcactg gccgaccagg ccgccctgac cggggagtcg atcaagccgg
     1801 gagacgtggt gatcaccggt gccgccatcc ctcccgcccc ggtcagcccg ggggacgatt
     1861 acggggtcga gatcaccggc ggcagctcgg tcacggtcaa actggtgtga acgaacgcgt
     1921 gggaaccaac tgcattgaag ttattcgttc catgtgcgga taacttcaac ccagaagtcc
     1981 ggacctcacg ggtcagcctg aaaatcgggg cgactggatt tgaaccagcg acctttcgac
     2041 ccccagtcga atgcgctacc aaactgcgcc acgccccgtg cgtgaccggg gactttagcg
     2101 ggagacggct tggcgccgct gtacggagca tgcgccccgg gaccgggtga agcgggcgac
     2161 gggaatcgaa cccgccctag gagcttggaa ggctcctgtg caaccaatac acctcgcccg
     2221 cagagaaatc gcgaccacca attatagggg ccgaatcctg actccgatcc gtcgaaaatc
     2281 gacccgggaa gcggggcacc caggactcga acctggaacg acaggaccaa aacctgcagt
     2341 gttgccaatt acaccaacgg ggaatggggt gtatgtccag cctaccgtgg ccgtcgtgat
     2401 gccctacggg atgatcgtgg tcgtccgggc gccgggaacc gggccggacg cgatgcgcgc
     2461 actgcggcac aggcccgttg tcgacaggcc cacggcgagt tcgagaccgt gctcgttgtc
     2521 cctggcgagg aacacacatg tcggaccgct gccactcacc atcgcgccga gggcgccata
     2581 gtcacggcct gcctcgagca gcagatccaa ctccgggcgc agtgcgatgg ccggttgctg
//
LOCUS       DAOQMF010000209         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_23567_length_2626_cov_3.124854, whole genome shotgun sequence.
ACCESSION   DAOQMF010000209 DAOQMF010000000
VERSION     DAOQMF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23567_length_2626_cov_3.124854"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="PLV77_04475"
     CDS             complement(<1..1775)
                     /locus_tag="PLV77_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15131.1"
                     /translation="MWLAALGLSFFALLLPGHSAAARAAGECTLIPGGTIGDPEPEPV
                     PDRAGSPTDRGPGAHGANPTLKGLPTRVDLRSPSMAQNRNHYFALKNGRLFVKPNYER
                     TGRRGSWRHVTTPSCLDGDIRSIDADDDELTAINSQNQIFGMDQALSSPSRFNWTSRW
                     GFPIWLGDGYTIPKDTVAWAWTVISPRESRYWVDPAGNQHTVGEGKVSHVLSLRGDHR
                     RITMNDPWLPQDRSYEVCGPRRGTFRISNLAASGSVLFVIGDHGDMYTRLWDFDIAGL
                     DNAFFDYSYEDQRGVEDPAIQLPGAHWVQQPKIRGEITNRISITTIGRGTLHRILRVE
                     GKNAKGLPGFWQKDLYTLNRGSSGWKFVATPKAPITGRWLDNSPRDMGAVGAGRPEGR
                     RYLASGANYLAEIPDFNVSCGRALLRVSFDDGRSIDLNLFLDDMVRIYPRARGLDGEP
                     RLVLGTIAVTPRARATGGDEARDFIRDVLGDRKFTTARITATTAGLEIPDLGLQFTAA
                     GEPSAGRVPDQGFKEFHVMGRKLGLAFTFETATESPASIVVSRIDGDGRGPVAARLTR
                     TGSFFWEPRRLADGRYRVEASSMGLG"
     gene            complement(1818..>2626)
                     /locus_tag="PLV77_04480"
     CDS             complement(1818..>2626)
                     /locus_tag="PLV77_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC15132.1"
                     /translation="EAAAIDLARCGAIVHIVARNPDKAEETRVEIQSAAPGAPVSVHL
                     CDLSLTTDIRRFTDAFLKSGDRLDVLINNAGVMPAEREYTDEGFELTFATNLIGPYLL
                     TELLLPRLREGDNPRVITMSSGGMYSSGFSFRDPQLAEKDYKPTSFYAHTKRGEVMIT
                     DAWQRREPEGGVTFCSMHPGWAVTPGMSAALPGFHKLTGPILRDSHEGVDTAIWLAGA
                     TPAEAPGGTFYEDRRPRTKQRMPGTAGSSEDGERLLELLAGITSGSDAEG"
BASE COUNT          423 a          853 c          900 g          450 t
ORIGIN      
        1 ccgagaccca tcgaactggc ctcgaccctg tacctgccgt cagccagccg ccgcggctcc
       61 cagaaaaacg acccggtgcg ggtgaggcgg gctgccaccg gtccgcgacc gtcaccgtca
      121 atccggctga ccacaatcga agccgggctt tccgtcgccg tctcaaaggt gaaggcgagc
      181 cccagcttcc gacccatcac gtggaactcc ttgaagccct ggtcaggtac ccgtccggcc
      241 gaaggttccc cggcggctgt gaactgaagg ccgaggtccg gaatctcgag cccggcggtc
      301 gtcgcggtga tccgcgcggt ggtgaatttg cgatcgccga gcacgtcccg gatgaaatcc
      361 cgggcttcgt cacccccggt cgcccgggcc cgtggcgtga ccgcgatggt tcccaggacg
      421 agccgtggtt cgccgtcgag gccgcgggcg cgcgggtaga tgcggaccat gtcgtcaagg
      481 aagagattca ggtcgatgct tcttccgtcg tcgaagctga cccgaagcag ggcccgcccg
      541 caggaaacgt tgaaatccgg gatctcggcc aggtagttgg ctccgctcgc gaggtaacgc
      601 cggccctcgg gccggcccgc cccgaccgcg cccatgtccc ggggcgaatt gtcgagccag
      661 cgaccggtga tcggcgcctt cggggtggcg acgaacttcc agccggacga gccccggttc
      721 agggtgtaga ggtccttctg ccagaacccg ggcagtcctt tcgcgttttt gccctcgacc
      781 cggaggatgc ggtgcagggt gccccggccg atcgtggtga tgctaatccg gttggtgatc
      841 tcgccccgga tcttcggttg ctggacccag tgggcgccgg gcagctgaat cgccggatcc
      901 tcgaccccgc gctggtcctc gtaggagtaa tcgaagaagg cgttgtcgag gccggcgatg
      961 tcgaagtccc agagccgggt gtacatgtcg ccgtggtctc cgatcacgaa aagcaccgaa
     1021 ccagaggcgg caaggttgct gatccggaag gttccgcggc gggggccaca gacctcgtaa
     1081 ctccggtcct ggggcagcca ggggtcattc atggtgatcc gccgatggtc cccgcgcagg
     1141 ctgagcacat gcgagacctt gccctcaccg accgtgtgtt ggttgccggc cgggtcgacc
     1201 cagtacctcg attcccgcgg cgagatcacg gtccaggccc aggccacggt gtccttgggg
     1261 atcgtgtagc cgtcacccag ccagatcggg aagccccagc gggaggtcca gttgaagcgg
     1321 gaggggctcg aaagcgcctg gtccatgccg aagatctggt tctgcgagtt gatcgcggtc
     1381 agctcgtcat cgtcggcgtc gatcgagcgg atgtcgccgt ccaggcacga cggggtggtg
     1441 acgtgccgcc agctcccgcg cctgccggtc cgctcgtaat tcggtttgac gaagagccgg
     1501 ccgtttttga gggcgaagta gtgattgcgg ttctgggcca ttgacggcga gcgcaggtcg
     1561 acacgggtcg gcaggccttt caaggtcggg ttggcgccgt gggcgcccgg accccggtcg
     1621 gtcggagaac cagcccggtc cgggaccggc tcaggctcgg ggtcgccgat cgtgccgccc
     1681 gggatgagcg tgcattcccc cgccgccctg gcggccgctg aatggccggg cagcagcagg
     1741 gcgaagaagg acagccccag ggccgccagc cacaaaatcg acagctgcgc gggcgggaac
     1801 cggcccggtt tcaatgatca cccctcggca tcggagccac tggttatacc agccagtagc
     1861 tcaagcagcc gttcgccatc ttcggaggag ccggcagtcc ccggcattcg ctgcttggtc
     1921 cggggccggc gatcctcgta gaaggttccg cccggcgcct ctgccggagt cgcgccggcg
     1981 agccagattg ccgtgtcgac accttcatgc gaatcccgca ggatcggacc ggtcagcttg
     2041 tggaagcccg gcagggcggc gctcatgccc ggtgtgaccg cccagcccgg atgcatcgag
     2101 cagaaggtca ccccgccttc gggttcgcgc cgctgccagg cgtcggtgat catgacctcg
     2161 ccccgtttgg tgtgggcata gaagctggtt ggcttgtagt ccttctccgc cagttgcggg
     2221 tcgcgaaagc tgaacccgct cgagtacatg ccgccggaac tcatcgtgat cacccgcggg
     2281 ttgtcgcctt ccctcagccg gggcagcaga agctcggtga gcaggtacgg accaatcagg
     2341 ttggtggcga aagtcagctc gaatccctca tcggtgtatt cacgctccgc cggcatcacc
     2401 ccggcgttgt tgatcagcac gtcaagccgg tcgcctgatt tgaggaacgc gtcggtgaaa
     2461 cgccgaatgt ccgtggtcag cgagagatcg cagagatgaa cgctgaccgg ggcaccggga
     2521 gcggcggact ggatttccac ccgggtttcc tcggccttgt ccggattgcg agcgacgatg
     2581 tggacgatcg ccccgcaacg ggcgaggtcg atcgcggcgg cttcac
//
LOCUS       DAOQMF010000210         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_23605_length_2623_cov_2.589564, whole genome shotgun sequence.
ACCESSION   DAOQMF010000210 DAOQMF010000000
VERSION     DAOQMF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23605_length_2623_cov_2.589564"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLV77_04485"
     CDS             <1..715
                     /locus_tag="PLV77_04485"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HNC15133.1"
                     /translation="AEADLARSKKRLALARKYLARHLVAVYMHGGEPDTLDMALGAGS
                     FDQLTTGTEYLTAIEDTNERLMDRVAELRRELDGKVDGLGDAKQAIDEHNSALAAARD
                     QIASVQAAAASSAATLAAENATRAGQITSLKGDIAGWQKQIEKQQQVSAEQAQVEVEQ
                     TLGGPYSIPTYIVMCESGGDYSAVNPSSGAGGAYQIMPSTWEAYGGTGLPQDASKAEQ
                     DRIAALIWADSGSSPWTCG"
     gene            complement(778..1236)
                     /locus_tag="PLV77_04490"
     CDS             complement(778..1236)
                     /locus_tag="PLV77_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC15134.1"
                     /translation="MIYLLRHGDAEPDQGSGDAARRLTEKGEAQALAAGAAMAKLGMT
                     VDTCLASPRVRAFDTATLACRSLGIKVEVSEPIGYGDYDSLELAAGRGDTLIVGHEPV
                     LSMEVARLTGANIKMKKGGLALIKGSHLHALLRPTELIEIATGGARSSNI"
     gene            1276..2073
                     /locus_tag="PLV77_04495"
     CDS             1276..2073
                     /locus_tag="PLV77_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC15135.1"
                     /translation="MTEAAAVPDFSTLLVSAEGPIGRLTLNRPEKLNALSGELLNELI
                     LASKWFTDRTGVKVVLVSGAGRAFCAGADLTSFSAAGSGPSEEERRDGADLGRRMADA
                     VTGMRPMTVAAIQGHCVGGGLVLAAACDLRIAAEDTRFSIPEVDLGIPLAWGGIPRLV
                     REIGPAATKELVLTCREFDAREARELGFLNRTVDPDRLEGQAWELAEKLASQPAYSLE
                     LTKRHVNAVAEESGSTADAITDADGLLGALGDPESLRKMGEYLARRG"
     gene            2179..2514
                     /locus_tag="PLV77_04500"
     CDS             2179..2514
                     /locus_tag="PLV77_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNC15136.1"
                     /translation="MSSSASSHGQVVHLHVHSEHSVLDGACNIKEMAARAAELEMPAL
                     ALTDHGVMNGALDMYKACRANDIKPILGIEAYYDFDLTDETKRQAGARMRNAGINIYF
                     RFDLADTSF"
BASE COUNT          467 a          859 c          866 g          431 t
ORIGIN      
        1 agccgaagcg gacctggccc gctcgaagaa acggctggcc ctggcccgca agtacctggc
       61 ccggcacctg gtcgccgtct acatgcacgg cggcgagccg gacacgctcg acatggccct
      121 tggcgccggc tcattcgacc agttgaccac cggcaccgag tacctgaccg cgatcgagga
      181 cacgaacgag cgcttgatgg accgggtcgc cgagttgcgc cgcgagctcg acggcaaggt
      241 ggacggcctc ggtgacgcca aacaggcgat cgacgagcac aactccgccc tcgccgccgc
      301 ccgcgaccag atcgcctcgg ttcaggcggc cgccgcatcc agcgccgcga cccttgccgc
      361 cgagaacgcc acccgggccg ggcagatcac ttcgctcaag ggtgacatcg ccggctggca
      421 gaagcagatc gaaaagcagc agcaggtaag cgccgaacag gcccaggtag aggtcgagca
      481 gaccctcggc ggcccgtact cgatcccgac ctacatcgtg atgtgtgaat ccggcggtga
      541 ttactcggcg gtcaacccct caagcggggc tggcggggcc taccagatca tgccttccac
      601 ctgggaggcc tacggtggca ccggactgcc ccaggatgcc tcgaaggccg aacaggaccg
      661 catcgccgcc ctgatctggg ccgactccgg ctccagcccc tggacctgcg gctgacttaa
      721 ctgcaagtcg ggcggggtcg gacccgccct ccaagttcaa cccgcccgat cagatcatca
      781 aatatttgat gatcgggctc cgccggtggc tatttcgatg agttccgtgg ggcggaggag
      841 ggcgtggagg tggcttccct tgatcagggc gaggccgccc tttttcatct tgatgttggc
      901 tccggtgagg cgggccacct ccatcgagag gaccggttcg tggccgacga tcagggtgtc
      961 gccgcggccg gcggcaagct cgaggctgtc gtagtcgccg tagccgatcg gctccgagac
     1021 ctcgaccttg attcccagcg accggcaggc aagggtggcg gtgtcaaagg cgcggacccg
     1081 gggcgaggcc aggcaggtat ccacggtcat gcccagcttc gccatcgccg cgccggcggc
     1141 cagggcctgg gcctcgccct tttcggtgag gcggcgggcg gcatctcccg aaccctggtc
     1201 gggttcggcg tcaccgtgac gaagcaggta gatcatgggt cgagattacc cgccaactgc
     1261 aactagaatc ggcccatgac cgaggccgcc gcggttcctg acttctccac cttgctggtt
     1321 agcgccgagg ggccgatcgg gaggttgacc ctgaatcgtc ccgaaaagct caacgcgctt
     1381 tccggtgaac tgctgaacga gctgatcctg gcctcgaaat ggttcacgga cagaaccggg
     1441 gtgaaggttg tcctggtcag cggtgccggg cgggccttct gcgccggggc cgacctgacc
     1501 tccttctcgg ccgccggttc gggcccgagc gaggaagaac gccgggacgg ggcggatctg
     1561 gggcggagga tggccgacgc cgtgaccggg atgcggccga tgaccgtggc cgcgatccag
     1621 gggcactgcg tcgggggagg cctggtgctc gccgccgcct gcgatttgcg cattgccgcg
     1681 gaggacacgc gcttttcgat cccggaagtg gacctcggca tcccgctcgc ctggggcggc
     1741 atcccccggc tggtcaggga gatcgggccg gccgcaacca aggaactggt gctgacctgc
     1801 cgcgagttcg atgcccggga agccagggaa ctcggctttc tcaaccggac ggttgacccg
     1861 gaccggctcg aaggccaggc ctgggaactg gcggagaagc tcgcctccca gccggcctac
     1921 tcgcttgaac tgaccaagcg gcacgtgaac gccgtggctg aagaatccgg ttcgacggcc
     1981 gatgcgatca ccgacgccga cggcctgctc ggagccctcg gcgaccccga atcgctccgg
     2041 aagatgggcg aatacctggc ccgtcgcggc tgaacgccag cggggatcgg gccttcccgc
     2101 aagttacggg aaaaagcgtc ccgagcggag caaatcgacc gggtttgcat catgcgcact
     2161 tgccatcatg gaccggcgat gagttcttcg gcatccagcc acggacaggt agtccacctt
     2221 cacgttcaca gcgagcattc ggtgctggat ggtgcctgca acatcaagga aatggcggcg
     2281 cgggcagctg aactggagat gccggctctg gccctgaccg accacggcgt catgaacggc
     2341 gccctcgaca tgtacaaggc ctgccgggcc aatgacatca agccgatcct cggcatcgag
     2401 gcctattacg attttgattt gacggatgaa accaagcgcc aggccggcgc ccggatgcgc
     2461 aatgcgggca tcaacattta cttccgcttc gatttggccg atacgtcctt ctgagcaggt
     2521 ttttttaata cttgttgcgg tgaacggctt tgctcgctca aaagctccca ccgcaacaag
     2581 cctatttgcc ttcggcaaac tgccgctcgg ccagcgcttt cag
//
LOCUS       DAOQMF010000211         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_23671_length_2619_cov_3.776911, whole genome shotgun sequence.
ACCESSION   DAOQMF010000211 DAOQMF010000000
VERSION     DAOQMF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23671_length_2619_cov_3.776911"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /gene="trpB"
                     /locus_tag="PLV77_04505"
     CDS             <1..1084
                     /gene="trpB"
                     /locus_tag="PLV77_04505"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNC15137.1"
                     /translation="ARKDPAFTGELNVLLRDYVGRPSPLYLASRLSEEAGRKVYLKRE
                     DLNHTGAHKINNAIGQALLARRMGKPRIIAETGAGQHGVASATACALLDLECVVYMGS
                     EDIRRQKPNVERMKLLGAEVVPVEAGARTLKEAVSAAIRDWVANVADTHYIIGSAVGP
                     APFPELVRDLQRVIGDEARDQALQAEGELPGRVIACVGGGSNAIGTFTAFIPDEEIEL
                     IGVEAAGHGLDTDKHGASLTAGNTSVLHGSLSAVLTDEEGQILEAHSVSAGLDYPGTG
                     PEHAHLRDIGRARYVAVDDRDAIAAFRRLSRLEGIIPALESSHAIAWLLGEGKIGDAD
                     GNYDVLCLSGRGDKDLAEVLGLEEAG"
     gene            1081..1899
                     /gene="trpA"
                     /locus_tag="PLV77_04510"
     CDS             1081..1899
                     /gene="trpA"
                     /locus_tag="PLV77_04510"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNC15138.1"
                     /translation="MTGAERIAAAFAAAGEEGRAALMPYMMGGFPDRETSTAIARSYA
                     ENGADLIELGVPFSDPLADGPVIHEAAVKALEAGTKLDDVLAICREVADRVPVVLMVY
                     TNTILGGAGPEAFAAKAAEAGACGVIVPDLPLGEDEKIRETMTAAGLAVVPLVAPTTL
                     GERRAEICRIASGFVYVVSSVGTTGERAEVPAHLKELVEDVESKSKVPVAVGFGIGSP
                     EHAAIVGQTADGVIIGSKLVRLAAEAAPGQAAEAVGEFMSEAAAAVASARSGVR"
     gene            1911..2090
                     /locus_tag="PLV77_04515"
     CDS             1911..2090
                     /locus_tag="PLV77_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15139.1"
                     /translation="MLIPVTFFVALALAVFSYSQGQGGPVAGLVFFGVLFIGALIHVC
                     QPLIEKGKALRPSKD"
     gene            2095..>2619
                     /locus_tag="PLV77_04520"
     CDS             2095..>2619
                     /locus_tag="PLV77_04520"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNC15140.1"
                     /translation="MKIGVLTGGGDCPGLNAVIRAVVLRGTREWGHEFTGYLDGWKGP
                     LENESIELGVGDVRGILAQGGTILGSSRTNPEAIEGGVDQVIDNVRAAGLDGLIAIGG
                     DDTLGFARILTESGLGVVGVPKTIDNDLNATDYTFGFDTAVNIVMESLDRLHTTATSH
                     HRCLVVEVMGRHAGW"
BASE COUNT          434 a          847 c          918 g          420 t
ORIGIN      
        1 ggcccgaaag gacccggctt tcaccgggga gctgaacgtc ctgttgcgcg actacgtcgg
       61 ccggccctcg ccgctctatc tcgcttcccg gctgagcgag gaggcgggcc gcaaggtcta
      121 cctgaagcgg gaggacctca accacaccgg cgcccacaag atcaacaacg cgatcggcca
      181 ggcgcttctg gcgaggcgga tgggcaagcc gcggatcatc gccgagaccg gggccgggca
      241 gcacggcgtc gcgagtgcca cggcctgcgc cctgctcgac ctcgagtgcg tcgtctacat
      301 gggcagcgag gacattcgcc gccagaagcc gaacgtcgaa cggatgaagc tgctcggggc
      361 cgaagtggtt ccggtcgagg ccggcgcccg caccctgaag gaggcggtca gcgccgcgat
      421 tcgcgactgg gtggcaaacg tcgccgacac ccactacatc atcggctccg cggtcggtcc
      481 ggctcctttt ccggaactgg tccgtgacct ccagcgggtg atcggggacg aagcccgcga
      541 ccaggccctg caggccgagg gcgagctgcc cggccgcgtg atcgcctgcg ttggaggcgg
      601 ttccaacgcg atcggcacct tcacggcatt cattcccgac gaggaaatcg agctgatcgg
      661 cgtcgaagct gccggtcacg ggctcgatac cgacaagcac ggagcttcgc tcaccgccgg
      721 caacacctcg gttctccacg gctcgctttc ggccgtgctg accgacgagg aagggcagat
      781 cctcgaagcc cattcggttt cggccgggct cgactacccc ggcaccggcc ccgagcacgc
      841 ccatctcagg gacatcggcc gggcccggta cgtggcggtc gacgaccggg acgcgatcgc
      901 ggccttccgg cgcctgagcc ggctcgaagg gatcattccg gcgctcgagt cgtcgcatgc
      961 gatcgcctgg ctgctcggcg aagggaagat cggtgacgcc gacggcaatt acgacgtgct
     1021 ttgcctgtcc ggccgcggcg acaaggacct ggccgaggtg ctgggcctgg aggaggccgg
     1081 atgacgggcg cggagcggat cgccgcggcc ttcgcggctg ccggagagga aggtcgcgcc
     1141 gcgctgatgc cgtacatgat gggcggcttt cccgaccggg agacttcaac cgcgatcgcg
     1201 cgctcctatg ccgaaaacgg ggccgacctg atcgaactcg gcgtgccctt ctcggacccc
     1261 ctggctgatg ggccggtgat ccacgaggcc gcggtgaagg cgcttgaagc gggtacgaag
     1321 ctcgacgacg tgctggcgat ctgccgcgag gtggccgacc gggtgccggt ggtgctgatg
     1381 gtctacacga acacgatcct cggtggagcg ggtccggaag ccttcgctgc gaaggcagcc
     1441 gaagccggcg cctgcggggt gatcgtgccc gacctgcccc tcggcgaaga cgagaagatc
     1501 cgcgagacga tgaccgccgc cgggctggct gtggttccgc tcgtggcgcc gacgaccctg
     1561 ggcgagagaa gagccgagat ctgccggatc gccagcggtt tcgtctatgt ggtctccagt
     1621 gtcggcacga ccggcgaacg agccgaggtg cccgctcacc tgaaggagct ggtcgaagat
     1681 gtggagtcga agtcgaaggt tccggtggcg gtcggattcg gcatcggatc acccgaacat
     1741 gcggcgatcg tgggccagac ggccgacgga gtgatcatcg gttcgaaact ggtccggctg
     1801 gccgccgagg cggcaccggg acaggcagca gaggcggtcg gtgaattcat gtccgaagct
     1861 gccgcggccg tggcatcagc ccgctccggt gtcaggtagg attcccgccg atgctgatcc
     1921 cggtcacatt cttcgtcgct ctcgccctcg cggtgttctc gtactcgcag ggccagggtg
     1981 gtccggttgc cggcctcgtc ttcttcggtg tgctgttcat tggcgcgttg attcacgtct
     2041 gccagccgct catcgaaaag ggcaaggcgc tccggccctc aaaggactaa ccccatgaag
     2101 atcggggtgt tgaccggcgg gggtgattgc cccggcctca atgccgtgat ccgtgcggtg
     2161 gtgctcagag gaacccgtga atggggtcac gagttcaccg gctatctcga cggctggaag
     2221 ggtccgcttg aaaacgagtc gatcgaactc ggggttggcg acgtccgcgg catcctcgcc
     2281 cagggcggaa ccatcctcgg ttcgtcccga accaacccgg aagcgatcga aggcggggtg
     2341 gaccaggtga tcgacaatgt ccgggcggcc ggcctcgacg ggctgatcgc gatcggcggc
     2401 gacgacaccc tcggcttcgc ccggatcctc acggagagcg gactcggagt ggtcggcgtg
     2461 ccgaagacga tcgacaacga tctgaacgcc accgattaca ccttcggttt cgacaccgcc
     2521 gtcaacatcg tgatggagtc cctggaccgg ctgcacacga ccgccaccag tcatcaccgc
     2581 tgcctggtgg tcgaggtgat gggtcgtcac gccggctgg
//
LOCUS       DAOQMF010000212         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_23753_length_2613_cov_2.859265, whole genome shotgun sequence.
ACCESSION   DAOQMF010000212 DAOQMF010000000
VERSION     DAOQMF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23753_length_2613_cov_2.859265"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..2362
                     /locus_tag="PLV77_04525"
     CDS             119..2362
                     /locus_tag="PLV77_04525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC15141.1"
                     /translation="MSPSSSPTRTGEAPGRAGANGDANPRPKRKDVPIRLRVGASEGL
                     YRIRPFEMVVRTHPPERPGEYRDVSELWTLDKDEFGSRGRIDTTMGVGMAAAAWAPLR
                     SLLEADVPDRLELSDAEVLDLLDGAAERLEASGIVLDWDPDLDQSLTGKVVVGSSGRL
                     SQSVPDVFDQNALQGFSWQLSLGGTPLSEEEMAVIADTDGPIARLREKWVLVPRVMAE
                     RARHPDLKPLTAIDALSVALTGSTVVDGQRVDVEAKGWVAQLREQVTGDSEQSLIEPP
                     ADLNATLREYQVEGMRWLHRMTSLGLGGCLADDMGLGKTVMLISLHLHRRLNPQTAGP
                     TLVVCPASLLGNWEREIQRFAPTEKVRRYHGPNRSLDQITSGFVLTTYGTMRLDAELF
                     EAVPWGLLVADEAQHVKNPRSAGAKALRTIPARSRVALTGTPVENNLSELWAILDWTT
                     PGLLGSLGAFRKKWADPIEVEGSQSATRLLNRLVRPFLLRRRKSDPGIAPELPPKTIT
                     DQPVELSPEQIKLYEAAVRENMLQIRTAEGINRRGLVLKLLTSLKQICNHPAQFLGEE
                     GGPLAERSGKLELLDELLDTILSEDSSVLVFTQYVAMARLLSRHLEERGIGAMLLHGG
                     TAVRQRERMVERFQDGEVPVFLLSLKAAGTGLNLTRADHVIHYDRWWNPAVEEQATDR
                     AHRIGQLNSVQVHRLIAEGTIEDRIASIHETKRELADAIVSTGEAALTELSNEDLAEL
                     VELRNAG"
     gene            2362..>2613
                     /locus_tag="PLV77_04530"
     CDS             2362..>2613
                     /locus_tag="PLV77_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC15142.1"
                     /translation="MGGQAEGSRTWRGQTIEERQAGRRERLIEAGIELFGTRGYANTP
                     VKAICEEAGLTERYFYEAFEDRESLLDEIYSMVLADCRRA"
BASE COUNT          486 a          869 c          875 g          383 t
ORIGIN      
        1 cctgatgctg aaagccgccg gcatcgccct tcagcagaaa aaagaagccg aagaagcggg
       61 ctgatcgccg ggcgtgaacg gccccgccgc ggcaaaggca tacaatgtct ggccgctttt
      121 gtctccgtct tcatcaccca ccagaaccgg cgaggcgcca ggtcgcgccg gcgccaatgg
      181 cgacgcgaac ccgcgcccga agcgcaagga cgttccgatc cgacttcggg tcggcgcttc
      241 cgaaggcctg taccggattc gccccttcga aatggtggtc cggacccacc cgccggagag
      301 gccgggcgag taccgggacg tctccgagct ctggaccctc gacaaggacg agttcggttc
      361 acgcggccgg atcgacacca cgatgggggt cggcatggcc gccgcggcct gggcgccgct
      421 ccgcagcctg ctcgaagcgg atgtgccgga ccggctcgaa ctcagtgacg ctgaggtgct
      481 ggacctgctg gacggcgccg ccgagaggct cgaggcatcc ggcatcgtgc tggattggga
      541 cccggacctg gaccagagcc tgaccggcaa ggtggtggtg ggaagcagcg gacggttgag
      601 ccagagcgtt cccgacgtgt tcgaccagaa cgcgctgcag ggcttttcct ggcagctctc
      661 gctcggagga actcccctga gcgaggagga aatggcggtg atcgccgata ccgacgggcc
      721 gatcgcccga ctccgggaaa aatgggtgct ggtgccgcgg gtgatggccg aacgggcgcg
      781 ccatcccgat ctgaagcccc tgaccgcgat cgacgccctc agcgtcgcct tgaccggcag
      841 cacggtcgtg gacggccagc gggtggacgt cgaagccaag ggctgggtag cccagcttcg
      901 tgaacaggtg actggcgaca gcgaacagtc gctgatcgaa ccgccggctg acctcaacgc
      961 gaccctgcgc gagtaccagg tggaaggcat gcgctggctc caccgaatga cctcgctcgg
     1021 tcttggcggc tgcctggccg atgacatggg cctcggcaag accgtaatgc tgatctcgct
     1081 tcaccttcac cggcggctca atccccagac cgcgggaccg acccttgtcg tctgcccggc
     1141 ctcgctgctt ggcaactggg aacgggagat ccagcgcttc gccccgaccg agaaggttcg
     1201 gcgctaccac ggcccgaacc gcagcctcga ccagatcacc agcgggttcg tgctgaccac
     1261 ctacggcacc atgcggctcg acgccgaact gttcgaggcc gtgccctggg ggcttctggt
     1321 cgccgacgag gcgcagcacg taaagaatcc gcgctcggcc ggtgccaagg cgctccgcac
     1381 gatcccggcc cgctcccgcg ttgccctgac cggcacaccg gtggagaaca acctctccga
     1441 gctctgggcg attctggact ggaccactcc cggcctgctc ggcagcctcg gtgcgttccg
     1501 caagaagtgg gccgacccga tcgaggtcga gggcagccaa tcggccaccc gtctgctcaa
     1561 tcgcctggtc cggccgttcc tgctcaggcg acggaagtca gatccgggca tcgcaccgga
     1621 gctgccgccg aagacgatca ccgaccagcc ggttgaactg agccccgaac agatcaagct
     1681 ctatgaagct gcggtccgcg agaacatgct gcagatccgt accgccgagg gaatcaaccg
     1741 ccgcggcctg gtgctgaagc tgctcacttc gctgaagcag atctgcaatc acccggccca
     1801 gttcctcggg gaagaagggg gaccgctggc cgagcgatcc ggcaagctcg aactgctcga
     1861 cgaactgctc gacaccatcc tcagcgagga cagctcggtg ctggtcttca cccagtacgt
     1921 ggcgatggcc cgtctgctgt cacgccacct cgaagagcgc ggaatcggcg cgatgctgct
     1981 ccacggcggt accgcggtga ggcaacgcga acgcatggtc gaacgcttcc aggatggcga
     2041 ggtaccggtc ttcctgcttt ccctgaaggc ggccggaacc ggactcaacc tgacccgggc
     2101 tgaccacgtg atccattacg accgctggtg gaacccggcg gtggaagagc aggcgaccga
     2161 ccgcgcccac cggatcggcc agctcaactc ggtccaggtc caccgcctga tcgccgaggg
     2221 cacgattgaa gaccgcatcg cctcgattca cgagaccaag cgtgaactcg ccgatgcgat
     2281 cgtcagcacc ggtgaagcgg ccctgaccga gctcagcaac gaagacctgg ccgaactggt
     2341 cgaactgagg aacgcgggct gatgggcgga caggccgaag gcagccgcac ctggcggggg
     2401 cagacgatcg aggaacgcca ggccggccgg cgggagcggc tgatcgaagc cgggatcgag
     2461 ctgttcggga ccagggggta tgcgaacacg ccggtcaagg cgatctgtga ggaagccggg
     2521 ctgaccgagc gctacttcta cgaggccttc gaggaccggg agagcctgct ggacgagatc
     2581 tactcgatgg tgctggccga ttgccgacgg gcg
//
LOCUS       DAOQMF010000213         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24202_length_2585_cov_3.009091, whole genome shotgun sequence.
ACCESSION   DAOQMF010000213 DAOQMF010000000
VERSION     DAOQMF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24202_length_2585_cov_3.009091"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLV77_04535"
     CDS             complement(<1..402)
                     /locus_tag="PLV77_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC15143.1"
                     /translation="MPAVVLALLSSVSYGFSDYLGGLKSRNIPVLTVLLVSQAAALVA
                     ITATLALAVGDIPGNRYLAYGVLAGIAEVVGLSALYHGLSVGKMSIVAAVAATAPVVP
                     VIASVISGDAPGALQWFGIVIAIGGVSLLALG"
     gene            547..>2585
                     /locus_tag="PLV77_04540"
     CDS             547..>2585
                     /locus_tag="PLV77_04540"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC15144.1"
                     /translation="MTRKLVLLFAAFAAIGLIGAGSAAAAPPPLGISCTVAGDNSLQC
                     GSTSPRSTAETWDGTPIDVNVALPDPAEFGTGPYPLVMMFHGYGQSKLPFAQMAHYTD
                     KGYAAFTMSDRGMAESCGSVASVAADPDGCDGQYIHMLDDRYEVRDAQYFAGELVDEG
                     WVDPARIAATGGSYGGGMSMALAALKDRTMLPNGFLVPWKSPAGTPISLAVATPLVPW
                     TDLAYSLSPNGRVLDYLRENPYDPEHIGIMKSSIINGLYLSGNAIGRYAPVGTYPQAD
                     MTGWRLMMDQGEPYQSDLAFSAMLSEITTYHSSYYIDDSVKPAPMLISLGFTDDIFGV
                     DEGIRYINRTLATYPDADIALMAADVGHQRAQNKAADGAAVFSLQDKWIDYYLTGAGA
                     KPANNVTAYTEVCPNAEPSAGPYTAANWASLAPGEITVEGGKADQTIEPDGGSADVAN
                     AYGVIANNACASPSGAREPGTANYESDPAPAGGFTLLGSPTVIADFEGGGRESEIAAR
                     LVDVAPDNTKQLVARQLYRPNASGYQVFQLHPGAWTFKEGHIARLELLPKDASTPPSS
                     LNLSNYGRPSDMQQTITVHDLVLRLPVIESPGGLGGLVKAPAAKILPDDRRSVDLAPG
                     YSSSQTMADWAASRPGPDPTPTVQKLKVSGPVSAKGTKATVKVSCPASAASCAKA"
BASE COUNT          476 a          886 c          821 g          402 t
ORIGIN      
        1 gccgagcgcg agcaggctta ccccgccgat cgcgatcacg attccgaacc actgcagcgc
       61 acccggagcg tcgccgctga tcaccgaagc gatgaccggg accaccggcg cggtcgcagc
      121 gaccgcggcg acgatgctca tcttcccgac cgaaagcccg tggtagaggg cagacagacc
      181 aaccacctcc gcgatcccgg ccagcacccc gtaggcgagg taccggttgc cggggatgtc
      241 accgaccgcc agcgcgaggg tcgccgtgat cgcgaccagg gcggcagcct gtgagaccag
      301 cagcaccgtg agcaccggga tgttgcggct cttgagtccg ccgagatagt cggaaaaacc
      361 gtaggaaacg ctggagagca gggcgagcac gaccgctggc attccgggca gcctatgtgg
      421 tggtgccccc gggatgccgt cgcgtctgtt cttgcattca ccttggtaga taaatgcagg
      481 actgcctgct ataagtttgg gtagtggagg aagcgagggg ctcctccatt gacgggagga
      541 gacccgatga ccaggaagct tgttctgctt ttcgccgcgt tcgcagcgat cggcctgatt
      601 ggagccggtt cggctgccgc ggcaccgccg ccgctcggca tctcgtgcac ggttgccggg
      661 gacaactcgc tccaatgcgg cagcaccagc ccgcgcagca cggcggagac ctgggacgga
      721 accccgattg acgtgaacgt cgccctgccc gaccccgccg aattcggcac cgggccgtac
      781 ccgctggtga tgatgttcca cggctacggt cagagcaagc tccccttcgc ccagatggcc
      841 cactacacgg acaagggtta cgcggccttc acgatgagtg accgcggcat ggccgagtcc
      901 tgcggcagcg tcgcctcggt cgcagccgat ccggatggct gcgacggcca gtacatccac
      961 atgctggacg accgttatga agtgcgggac gcccagtact ttgcggggga gctggtcgac
     1021 gagggctggg tcgatccagc caggatcgcc gcaaccggcg gttcgtacgg cggtggcatg
     1081 tcgatggcgc tggccgcgct gaaggaccgc acgatgctgc caaacggttt cctcgtgccc
     1141 tggaagagtc cagccggcac gccgatcagc ctggcggtgg cgactccgct ggtcccctgg
     1201 accgacctcg cctattcact ctcgcccaac ggcagggtcc tggactatct ccgcgagaat
     1261 ccttacgacc ccgagcacat cggcatcatg aagtcgtcga tcatcaacgg gctgtacctc
     1321 tcgggcaacg cgatcggcag atacgcgccg gtcggaacct atccccaggc cgacatgacc
     1381 gggtggcgac tgatgatgga ccagggcgag ccgtaccaga gcgacctggc tttcagcgca
     1441 atgctcagcg agatcaccac ctatcactcg tcctattaca tcgatgacag cgtgaagccg
     1501 gccccgatgc tgatctccct gggtttcacc gatgacattt tcggtgtcga tgagggaatc
     1561 aggtacatca atcggactct cgccacctac cccgacgccg acatcgcact catggccgct
     1621 gacgtcggcc atcagcgggc tcagaacaag gcggcggacg gtgccgccgt gttcagcctc
     1681 caggacaagt ggatcgatta ctacctgacc ggggctggcg ccaagccggc caacaacgtg
     1741 accgcctaca ccgaggtctg ccccaacgca gaaccctcgg ccggtccgta cacggccgcc
     1801 aactgggctt cgctcgcccc gggtgagatc acggtcgagg gaggcaaggc agaccagacg
     1861 attgagcccg atggtggcag tgccgatgtc gccaacgctt acggggtcat cgccaacaat
     1921 gcctgcgcca gcccgagcgg cgccagggaa cccggcacgg cgaactacga atccgacccg
     1981 gcaccggccg gtggcttcac tctgctcggc tcgccgaccg tgatcgccga cttcgaaggg
     2041 ggcggcagag aatccgagat cgccgcgcgc ctggttgacg tcgcgcccga caacaccaag
     2101 cagctcgtgg cccgccagct ctaccggccc aacgcgagcg gctaccaggt gttccagctc
     2161 catcccggcg cctggacctt caaggagggt catatcgccc ggctggagct gctgccgaag
     2221 gacgcgagca cgccgccttc gtcactcaac ctgtccaatt acgggcgacc ctccgacatg
     2281 cagcagacga tcactgtcca tgacctcgtc ctccggctgc cggtcatcga gagcccgggc
     2341 gggctcggtg gtctggtcaa ggccccggcc gcgaagatcc tgccggatga ccgccggtcg
     2401 gttgacctcg ctcccggtta cagcagctcg cagaccatgg ctgactgggc tgcgagccgg
     2461 cccggtcccg acccgacccc gaccgttcag aagctgaagg tcagtggtcc ggtctcggcc
     2521 aagggcacca aggccacggt caaggtcagc tgcccggcca gcgccgcctc ctgcgccaag
     2581 gccag
//
LOCUS       DAOQMF010000214         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24212_length_2584_cov_3.839067, whole genome shotgun sequence.
ACCESSION   DAOQMF010000214 DAOQMF010000000
VERSION     DAOQMF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24212_length_2584_cov_3.839067"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PLV77_04545"
     CDS             complement(<1..928)
                     /locus_tag="PLV77_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC15145.1"
                     /translation="MDSGTGQLFESEASFNGAVEVVHVIWAAEDDGFAVVEVRDDSGE
                     EFVATGTIGHLKPGERAKLTGQWSEHQKYGPQVQVFTALPMDPSDRAGQVAYLSSLRH
                     IGPIRAEALCELHGPEVLEKIAADPEGILGSLPGLGAKQREAALESWYETRAVRDLHV
                     ELAPHGLAHLAGKIHARFGDKAMRTIREDPYSLTEIDGVGFARADTIAIAAGFPPESD
                     RRAQAAAWYLLGEAERRGHTHLPLNELVAQATKLLGYRPEPEVLTSASGVIAEEDRIY
                     RQSTLDRECWLAGDLRERAAGEAVLDHSPEPGA"
     gene            989..1444
                     /locus_tag="PLV77_04550"
     CDS             989..1444
                     /locus_tag="PLV77_04550"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC15146.1"
                     /translation="MAEPSKPLGDAVAEATEAASLPKADRPKKRTAKPQDVFGGEMRR
                     RDLLPLLIFHLIRAEPSYGNQLIDAIETLTQGVISVNPNTMYPLLRDLEGKGMIEGSW
                     EHPDKRTRRFYSITESGEAAYQELLPEIEPFLDSVIRSVTMIKAEVYGS"
     gene            1532..1735
                     /locus_tag="PLV77_04555"
     CDS             1532..1735
                     /locus_tag="PLV77_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC15147.1"
                     /translation="MPTGTVKWFSDEKGFGFITPDDGSKDVFVHHSAIQADGFRTLAE
                     GAKVSYESEEGPKGPAAASVETI"
     gene            1834..>2584
                     /locus_tag="PLV77_04560"
     CDS             1834..>2584
                     /locus_tag="PLV77_04560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC15148.1"
                     /translation="MYSTGGVADRFIPLWSRIEGRLGGSLPAAIRFWDGSEIGPGEAG
                     SCPDVIVLRSKRAMTYVVTRPDQLGVARAFVSGDLVLEGSIERVMAAGARLYGFDFTM
                     QDKLEAVKLAVGLGAIKVPPPKPPESEARPKGRLHSLRRDSESIQHHYDVSNDFYRLI
                     LGPTMVYSCAYFNSADDSLEEAQTRKLDVVCQKLRLEPGERLLDIGCGWGSMAIHAAS
                     NYGVKVVGVTLSIAQAERARQRVAEAGLEDRI"
BASE COUNT          444 a          827 c          866 g          447 t
ORIGIN      
        1 ccgcgcccgg ttcgggtgaa tggtccagga ctgcttcacc ggcagcgcgt tccctgaggt
       61 ctccggccag ccagcattca cggtcgaggg tgctctggcg gtagatccgg tcctcctcgg
      121 cgatcactcc cgaagccgag gtcagaactt ccggttccgg gcggtagccg agcagctttg
      181 tggcctgggc caccagttcg ttcaggggca ggtgggtgtg gccacggcgc tcggcctcgc
      241 ccagcaggta ccaggcggcg gcctgggccc ggcggtcgga ttccggcggg aagccagcgg
      301 caatcgcgat cgtgtcggcc cgggcgaaac cgaccccgtc gatctcggtc agcgaatacg
      361 ggtcttcgcg gatggtccgc atcgccttgt cgccgaagcg ggcatggatc ttgccggcca
      421 gatgggcgag accgtggggt gccagctcga cgtggaggtc ccgcaccgcc cgggtttcgt
      481 accagctctc gagcgccgcc tcgcgctgct ttgccccgag cccgggcaag gaaccgagga
      541 tcccctcggg gtcggcggcg atcttctcca gcacttcggg gccatggagt tcacagagag
      601 cctccgcccg aatcgggccg atgtgacgga gcgagctgag atatgcgacc tggccggccc
      661 ggtcggaggg atccatcggc agggccgtga acacctggac ctggggcccg tacttctggt
      721 gctcgctcca ttgcccggtc aatttggccc gctcacccgg tttcaggtgg ccgatcgttc
      781 cggtggcgac gaattcctca ccggaatcgt cgcgaacttc gaccaccgcg aagccgtcat
      841 cctcggccgc ccagatcaca tggacaacct cgaccgcgcc gttgaacgag gcttcgctct
      901 cgaagagttg tcccgtgcct gaatccaccc gctgaggtta atggtcctcc cggttaacaa
      961 cggcgggacg ggtttagact cgcctcctat ggccgagccg tccaagccgc ttggagatgc
     1021 ggtcgctgaa gcgaccgagg ctgcgagcct gcccaaagca gaccgcccga agaagcggac
     1081 ggcaaagccg caggacgtgt ttggtggcga gatgcgccgg cgggatctcc tgccgctgct
     1141 gatcttccac ctgatccggg ccgagccttc ctacggcaac cagctgatcg acgcgatcga
     1201 gaccctgacc cagggtgtga tctcggtcaa cccgaacacg atgtacccgc tgcttcgcga
     1261 cctcgaaggc aaggggatga tcgaaggcag ctgggagcac cccgacaagc ggacccgccg
     1321 cttctactcg atcaccgaat ctggcgaagc cgcttaccag gaactgctgc ccgagatcga
     1381 gcctttcctg gattcggtga tccggtcggt gaccatgatc aaggccgagg tttacggctc
     1441 ctgaagcgcc gcccggcgct caagtggtaa tttgccccgc gtcaggccgg gaggagagaa
     1501 ccggcaccgc atcatgatgt tggaggaagt tatgccgact gggacagtca agtggttcag
     1561 tgacgagaag ggcttcggat tcatcacacc tgatgatggc tccaaggacg tcttcgtcca
     1621 ccactccgcg atccaggccg acgggtttcg caccctggcc gagggcgcca aggtgagcta
     1681 cgagtccgaa gagggcccga agggccccgc agccgcttcc gtcgagacca tctgaacccg
     1741 gtctcggaag ctgttcgaaa cggccgcccc cgggcggccg ttttcattgc agctccttga
     1801 gccgaggtcg ggccgggagt aactttcgtg ggcatgtaca gcacaggtgg tgtcgccgat
     1861 cgtttcattc ctctctggtc ccggatcgaa ggcaggctgg gcgggtcgct cccggccgcg
     1921 atccgcttct gggacggcag cgagatcgga ccgggcgaag cgggctcctg ccccgacgtg
     1981 atcgtgctgc gaagcaagcg ggcgatgacc tatgtggtga cccggccgga ccagctcggg
     2041 gtggcccggg ccttcgtctc cggtgacctc gtgctcgaag gcagcatcga gcgggtgatg
     2101 gcggccggag cccggcttta cggatttgac ttcacgatgc aggacaagct cgaggcagtg
     2161 aagctggcgg tcggtctggg agcgatcaag gtgccgccgc cgaagccgcc ggaatcggag
     2221 gccaggccga agggaaggct gcattcgctc cgtcgcgact ccgagtcgat ccagcatcac
     2281 tacgacgtct cgaatgattt ctaccggctc atcctcggcc cgaccatggt ctactcctgt
     2341 gcctacttca actcggccga cgacagcctc gaagaggccc agacccgcaa actggacgtg
     2401 gtctgccaga agctcaggct cgaaccgggc gagcggctgc tcgacatcgg ctgcggctgg
     2461 ggatcgatgg cgattcatgc ggcatccaac tacggagtga aagtggtcgg ggtcaccctt
     2521 tcgatcgccc aggcggagcg ggccaggcag cgggttgccg aagcggggct cgaggacagg
     2581 atcg
//
LOCUS       DAOQMF010000215         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24308_length_2578_cov_3.887039, whole genome shotgun sequence.
ACCESSION   DAOQMF010000215 DAOQMF010000000
VERSION     DAOQMF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24308_length_2578_cov_3.887039"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLV77_04565"
     CDS             complement(<1..260)
                     /locus_tag="PLV77_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNC15149.1"
                     /translation="MTVRFNSHLTSIPGYSPGVPKGHSAEDVASSNLAQLASNESPFA
                     PLPEVVEAITRAAKAMNRYPDPAATRLRQRLADRHEIEPGQVS"
     gene            complement(289..675)
                     /gene="aroH"
                     /locus_tag="PLV77_04570"
     CDS             complement(289..675)
                     /gene="aroH"
                     /locus_tag="PLV77_04570"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNC15150.1"
                     /translation="MTDPEAEGMRVAAIRGAVQAENDADGILSATEELIREIISVNDL
                     EPDRMISAIFTTTEDLDAEFPAAAARRMGLNEVPLMCAREIPVPGAMPGVIRVMLHTY
                     LPAGAKARHVYLGETRNLRADLDAAQ"
     gene            complement(668..1366)
                     /locus_tag="PLV77_04575"
     CDS             complement(668..1366)
                     /locus_tag="PLV77_04575"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNC15151.1"
                     /translation="MRLAKYLAHAGVASRRKSEEIVAAGRVRVAGMVITDPAFGVEGG
                     EEVRVDGHQVEPEGREVWMLNKPLDVISTADEPGKRIAVVDLVDSNARLYPVGRLDAD
                     STGLILLSNDGELANRLTHPRFEVEKTYRVKLKKPVTPEAIDQLQRGVRLDDGMTAPA
                     TVKKLGEAELDVTIHEGRNRQVRRMAEAIGNEVVALQRISLGSLKLGRLGIGKCRLLD
                     EREVERLWKDAGDD"
     gene            complement(1389..2546)
                     /locus_tag="PLV77_04580"
     CDS             complement(1389..2546)
                     /locus_tag="PLV77_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15152.1"
                     /translation="MKKFAVLLALLASVFAVAACGSNNNPTVTPDTPASAMDEVCTDT
                     NADFEAMGTRGLTMDGIAAEFEGYAEVRQAVVDGFNELNTDDQAEPQLQKYIDASEQV
                     IAQDKAIAKAAAEGDQKAMNEAFAAQTKAFNERDKVAKEIGTEVCGQTPEIKVEKTGT
                     APPSDLNVVEPKNTVEDAADNYIEATASGNCGLINANRHTDAGDLDEASCKAAGQALK
                     GARVIGTESYGPVAQAEILSGNGTHYPTYFVTDLDGNLRYGGDAIHDSGGLRPAPEGN
                     DAEATVEATVNAIRDNDAAAFNETLAFEDSPFWIKGDTVEKFGEGAYNEEFIKDVKDG
                     DADPIQLGLNSTWAFYFLEGSKYDWVLSFTHNPGIGGHYAFTGYYPIPKPN"
BASE COUNT          352 a          833 c          865 g          528 t
ORIGIN      
        1 gatacctgtc cgggctcgat ctcgtgccgg tcggccagtc gctggcgcag cctggtcgcg
       61 gccgggtccg ggtaccggtt catcgctttg gccgccctgg tgatcgcctc caccacttcg
      121 ggcagcgggg cgaacggtga ctcgttggaa gcgagctggg ccagattgga gctggcgacg
      181 tcttcggcgc tgtggccctt cgggactccg gggctgtagc cggggatcga agtcaggtgg
      241 gagttgaaac gaacggtcat cgtgtatccg tgggttgagg gcgggctgct actgggccgc
      301 gtcgaggtcg gcccgcaggt tgcgggtctc gccgaggtag acgtgacggg ccttcgcccc
      361 ggccggcagg taggtgtgga gcatgacccg gatcaccccg ggcatcgcgc ccggcaccgg
      421 aatttcgcgg gcgcacataa gcggcacctc gttcagaccc atgcgacggg ctgccgccgc
      481 cgggaactcc gcgtcgaggt cctcggtggt ggtgaagatc gcgctgatca tccggtccgg
      541 ctcgaggtcg ttgacggaga tgatctccct gatcagctcc tcggtcgcgg acaggatccc
      601 gtcggcgtcg ttctccgcct ggaccgcgcc gcggatcgcc gcgacccgca tgccttctgc
      661 ctctggatca gtcatcgcct gcatctttcc agagccgctc cacctcgcgt tcgtcgagca
      721 acctgcactt gccgatcccg agtcgcccga gcttcagcga cccgagcgaa atccgctgca
      781 gagcgaccac ttcgttgccg atcgcttcgg ccatccgtct cacttgccgg ttgcggcctt
      841 cgtggatcgt gacgtccagt tcggcctcgc ccagcttctt cacggtggcc ggcgcggtca
      901 tgccgtcatc caggcggact ccgcgctgga gctgatcgat cgcttccggg gttaccggct
      961 tcttcagctt cacccggtag gtcttctcga cctcgaagcg cgggtgggtg agccggttgg
     1021 ccagctcgcc gtcgttgctg agcaggatca ggccggtcga gtcggcgtcg agccggccga
     1081 ccgggtagag ccgggcgttg ctgtcgacca ggtcgacgac ggcgatccgc ttgcccggct
     1141 cgtcggcggt ggagatcacg tcgagcggct tgttgagcat ccagacctca cggccctcgg
     1201 gctcgacctg atggccgtca acccggacct cctcgccacc ctcgaccccg aaggccgggt
     1261 cggtgatcac cattccggcc acccgaaccc ggccagcggc gactatctcc tcggatttcc
     1321 ttctcgaggc gacgcctgca tgggcgaggt acttggccag ccgcatggcc ggaactattt
     1381 cagccggatc agttcggctt gggaatcggg tagtagccgg tgaaggcgta atggccaccg
     1441 atgccggggt tgtgggtaaa cgaaaggacc cagtcgtact tgctgccttc caggaagtag
     1501 aaagcccagg tcgagttcag cccgagctga atcgggtcgg cgtccccgtc cttgacgtcc
     1561 ttgatgaatt cctcgttgta ggcaccctca ccgaacttct ccacagtgtc gcccttgatc
     1621 cagaacgggc tgtcctcgaa ggccagggtc tcgttgaagg cggcggcatc gttgtcgcga
     1681 atcgcgttga cggtcgcctc gactgtcgcc tcggcgtcgt ttccttccgg ggccggccgc
     1741 agtccgccgc tgtcgtggat cgcgtcacct ccataccgga ggttgccgtc aaggtcggtg
     1801 acgaagtagg tcggatagtg cgtgccgttt ccgctgagga tctcggcctg ggccacgggg
     1861 ccgtaactct cggtgccgat caccctggcg cccttcagag cctggccggc tgccttgcag
     1921 ctggcttcat cgaggtcacc ggcgtcggtg tgccggttgg cgttgatcag gccgcagttg
     1981 ccggacgcgg tcgcctcgat gtagttgtcg gcggcatcct cgaccgtgtt cttcggctcg
     2041 acgacattga ggtcggaagg cggcgcggtc ccggtctttt caaccttgat ctcgggtgtc
     2101 tgaccgcaga cctcggtgcc gatctccttc gccaccttgt cgcgctcgtt gaatgccttg
     2161 gtctgggcgg cgaaagcctc gttcatggcc ttctggtcgc cttcggcggc ggccttggcg
     2221 atcgccttgt cctgggcgat gacctgctcg gaggcgtcga tgtacttctg gagctgcggc
     2281 tcggcctggt cgtcggtgtt caactcgttg aacccgtcga cgactgcctg ccgcacctcg
     2341 gcgtagccct cgaactcggc cgcgatcccg tccatggtca ggccgcgggt gcccatcgct
     2401 tcgaagtcgg cgttggtgtc ggtgcagacc tcgtccatcg ccgaggcggg cgtgtccggc
     2461 gtgacggtcg ggttgttgtt gctgccacag gcggcgacgg caaaaacgga tgcgagcaag
     2521 gcaagcagga ctgcgaactt cttcaaagga ctggtctccc tggcttagga ctgggcgt
//
LOCUS       DAOQMF010000216         2577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24328_length_2577_cov_2.997224, whole genome shotgun sequence.
ACCESSION   DAOQMF010000216 DAOQMF010000000
VERSION     DAOQMF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24328_length_2577_cov_2.997224"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLV77_04585"
     CDS             complement(<1..748)
                     /locus_tag="PLV77_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNC15153.1"
                     /translation="MPARISAPIVFSALCLLGTLTVALSAVALIGTFISGDAPWAPVQ
                     ACCEAAIAKVSFGWLLLVAVGLISIVALVRLAFSSWLTIKANARVHSLRKLASTEKVA
                     GHDCLVLEDARPLAFCGGLVRPRVFLSRNLINELEPAELAAVVAHEAHHQNRRDPLRN
                     SITRVMAYGFFFLPMLREFGQKCVERSEIAADQAAVAGGESERKALASALLSLHGRES
                     EGVAVSLSPDRIGVLAGEKPSWIPSRNGALL"
     gene            complement(778..1710)
                     /locus_tag="PLV77_04590"
     CDS             complement(778..1710)
                     /locus_tag="PLV77_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC15154.1"
                     /translation="MLALAGCGDSNTETSEKDAAPAVDFPTAKNTSIEQLAAMAAPTD
                     IQFAPSGKVVEPGKNRYGFAAFDSQGQQQTDLDVALYATSGPKSKVLGPFPAKVESLE
                     TDPSFRAQTTSQDPGAAKAVYVVPNLNLPKAGNWYLLALAKSETGFVGAQLPNVVVRK
                     SNVVRPGEKAPAVSTPTAADVGGNLEKIDTRIPPDSLHAADFSSVVGEKPVILLFSTP
                     QLCESRVCGPVVDVQAQVAEETGDDVEFIHSEIYNDNDPNEGVNRQVQAFRLPTEPWL
                     FAIDENGIVRKRIEGAFGLGELREAVQSIRSESQ"
     regulatory      complement(2300..2447)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          485 a          791 c          810 g          491 t
ORIGIN      
        1 tcaagagggc tccgttccta cttggaatcc aggaaggctt ctcacccgcg agtactccaa
       61 tccgatcggg tgacaaggac actgccactc cctcgctttc gcgtccatgg agcgagagga
      121 gggcgctcgc aagtgccttt cgttcgctct caccgccggc taccgcagcc tggtcggcgg
      181 caatttcgga gcgttcaaca cacttctgac cgaactcccg aagcattggc aggaagaaga
      241 acccgtatgc catgacgcgt gtgatcgagt tccgcagggg atcgcgccgg ttctgatgat
      301 gggcctcatg agcaacgacg gcagcaagct ctgccggctc tagttcgttg atgaggtttc
      361 ggctgaggaa gacccgcggg cgaaccagcc cgccgcagaa cgcaagcggt cgagcgtcct
      421 ccagaaccag gcaatcgtgg cccgcgacct tctctgtcga agccagcttt ctaagggagt
      481 gaacacgcgc gttggccttg atcgtcagcc aactgctgaa ggccaggcga accaacgcga
      541 caatcgagat cagtccaacc gccaccaaga ggagccaccc gaacgaaacc tttgcgatgg
      601 ccgcttcaca gcaagcctga acgggagccc acggggcgtc accactgatg aaggttccga
      661 tcagcgcgac cgccgaaagg gccacggtca gcgtgcccag cagacaaagg gccgagaaga
      721 cgattggtgc ggaaatgcgc gcaggcactc gagagaggtt accgacggca ggccggatca
      781 ctggctttcc gatcgaatgg attggactgc ttcgcgaagc tcacccagac cgaaggcgcc
      841 ctcgatccgc ttccggacga tcccgttctc gtcgatggcg aatagccaag gctcggtggg
      901 caatcggaat gcctgaacct gccggtttac gccctcgttg gggtcgttgt cgttgtagat
      961 ctctgagtga atgaactcca cgtcatcgcc ggtttcctcg gcgacctggg cctggacgtc
     1021 caccaccgga ccgcagacgc ggctttcgca gagctgggga gttgaaaaga gcaggatcac
     1081 tggcttctcc ccgacgaccg aggagaagtc ggcagcgtgg agtgagtctg gcgggatgcg
     1141 ggtgtcgatc ttctcgaggt ttcccccgac gtcggccgcc gtgggtgtcg agactgccgg
     1201 ggctttctca ccggggcgga caacattcga tttgcggaca actacgttcg gaagctgggc
     1261 gccgacaaag ccggtttcac tcttggcgag cgcgagcagg taccagttgc ctgccttggg
     1321 caagttgagg ttcggtacga cgtagaccgc cttggcggcg ccgggatcct gggaggtagt
     1381 ctgggctcga aacgaagggt cggtttcgag cgactcgacc ttggccggga agggccctag
     1441 aactttcgac ttcggtccgc tcgtcgcata cagcgcgacg tcgagatccg tctgttgttg
     1501 tccctgcgag tcgaaggccg cgaacccgta gcggttcttt cctggctcga ccacctttcc
     1561 cgacggtgcg aactgaatgt cagtcggtgc cgccatggcg gcgagctgtt cgatagaggt
     1621 gttcttggcc gttggaaagt caacggcggg ggcagcgtcc ttctcgcttg tctcggtgtt
     1681 cgaatcgccg cagccggcta gagccagcac gctcagcgcc gccagaagaa cgaggctcca
     1741 ttgtcggccg ggacctagcg agctgaaccc cacttgcgga ggtagggcgt cgggttgctc
     1801 acgcttccac cgcggtacca gccgggacgg gtccacatct caaagtggag atggcaggcg
     1861 gtcgagcgcc cggtcgaccc gactagtccg attcgctgac ccgcccttac gagttggccc
     1921 gcgcggatcg ccggtgtgcg gagcatgtgc atgtacacgt agtcgaactt cgatgacgtc
     1981 ccgcgaatca cggcatagtt gccagccccg tcagcctggt agtcgacgag acgaactctt
     2041 ccagtttgag ccgcaactac tggcctggtg cacttcgcga gaagatcctg gccctgatgt
     2101 ccccggcctg ctccgagccc gtcgccccag gtgactgctc cacggatcgg gaacgctccc
     2161 ttggccggag ccgggctcgg agaagcgggt gacaaacctc ccgtcgaggc gcttgcgatg
     2221 ccggagagca ttcctagagc tccaaaggtc aacaacgccc taatggccaa ccgtccgatg
     2281 acggaatcgt gattcattcc ttctctcttt ctcgcgcctt cggggttagc tgacgggctc
     2341 gcgctgaaag aggtgcgcta cgcagagtgt gcgattcgcc ccagagactt gatggcctca
     2401 agtgggtccc ccgatccgga acactccgga ttcggctcat tggatttctc acatactacg
     2461 acactacgta gtagttccct ttatggggtc ccgccggccc tccggccggg ggcgcccttc
     2521 cggattgtgg gtgggccggc gggatccaga tcatagcgat ccctacgaca gtctgta
//
LOCUS       DAOQMF010000217         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24544_length_2566_cov_2.655117, whole genome shotgun sequence.
ACCESSION   DAOQMF010000217 DAOQMF010000000
VERSION     DAOQMF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24544_length_2566_cov_2.655117"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..374
                     /locus_tag="PLV77_04595"
     CDS             225..374
                     /locus_tag="PLV77_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15155.1"
                     /translation="MPLALLLIVIGIVLALLVNWALGIICIVIGLVLLIWPAVTGRGS
                     SGSRV"
     gene            complement(469..1203)
                     /locus_tag="PLV77_04600"
     CDS             complement(469..1203)
                     /locus_tag="PLV77_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNC15156.1"
                     /translation="MDPGSVDPADAELIRSNVARVRERMAAACERAGREPDSVRLLLA
                     TKTVEPARIRVAIEAGETLVAENRVQEVRPKFEALEDLRYERHFIGHLQSNKINALVP
                     YISCLETLDRLSLARKLENRLAREGETLEVLIQVNTSGEPTKAGLDPDEVEPFIRTIA
                     GLDALKVRGLMTIGLNAEPEVARPSLASLREIAGRIREENIEGVAMEELSMGMTGDLE
                     VAIEEGSTIVRVGSAIFGSRPPLQSP"
     gene            complement(1209..>2566)
                     /locus_tag="PLV77_04605"
     CDS             complement(1209..>2566)
                     /locus_tag="PLV77_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNC15157.1"
                     /translation="QATGRLKMKHGDSAFVCLSWGEGIGPHPRSYPEATERIESSRDL
                     WRDWLAQGTFPDHPWRIHLQRSALVLKGLTYTPSGAMLAAATTSLPETPGGERNWDYR
                     YSWIRDSTFALWSMHSLGFEQEARDFMRFVRDRAMESGGDLQIMYGIGGEHDLTEHTL
                     DHLSGYGGAKPVRTGNGAFDQRQNDVWGALLDSIYVHRKALGPGAINDETRDLVRRLV
                     ENALEAWPKPDQGIWEARGEPRHYVSSKLMIWVAVERASRLAAEFGVKENADRLRAKA
                     AEIKEEIIEKGCRDGVFRQHYETDALDASVLLMPLVRFLPPDDPRIRDTVLAIRDGLD
                     DRGLILRYITDETDDGLHGEEGTFLICSFWMVAALSEIGEKSAAKKLCERLLAASGDL
                     DLYAEELEASSNRHLGNFPQAFTHLALINAVSHVIADERRVDSAPGTPTAVFTEIREM
                     D"
BASE COUNT          401 a          836 c          855 g          474 t
ORIGIN      
        1 gcggcaatcg aaacggctcg cgaagccggt ctccaccggg tcgtctccct ggccctgccg
       61 gacaaccttg cctcgcgaag agttatggaa aaggccgggc tccagtacgt cgccgagatc
      121 gagcacgtcg gcctgccgca cctgctcttc gagctggacc tcgggtagcc cgcattcgag
      181 tggcccgcgg gtttgccggg cgccgttccg ggtacctccc aggcatgcca ctagcactgt
      241 tgctcatagt catcggaatt gtcctggccc tgctggtcaa ctgggcgctg ggcatcatct
      301 gcatcgtgat cgggctggtc ctgttgatct ggcccgcggt cacaggaaga ggaagcagtg
      361 gctcaagggt ctgactcgcc gggtagcgcc cggccgatca ggcacagccg attttgaccg
      421 aagggccccg ggaagccggg gcccttcgca ttcgtcggtc gctgaacctc agggtgattg
      481 aagcgggggg cgggagccga agattgcgct gcccacccgg acgatggtcg agccctcctc
      541 gatcgccact tcgaggtcgc cggtcatgcc catcgagagt tcttccatcg ccacgccttc
      601 gatgttctct tcgcggatcc ggccggcgat ctcccgcagg ctggccagcg aagggcgggc
      661 cacctccggc tcggcattga ggccgatcgt catcaaaccg cggaccttca gcgcgtcgag
      721 tccggcgatc gtacgaatga acggttcgac ctcatccgga tccagcccgg ccttggtcgg
      781 ctcgcccgag gtgttgacct ggatcagcac ctccagggtt tcgccttccc gcgcgagccg
      841 gttctcgagt ttgcgggcca gcgagagccg gtccagggtc tccaggcagc tgatgtaagg
      901 gaccagcgcg ttgattttgt tggactggag gtggccgatg aagtggcgct cgtaccgaag
      961 gtcttcaagt gcttcgaact tgggtcggac ctcctggacc cggttctcgg cgaccagggt
     1021 ctcgccggct tcgatcgcca cgcggatccg tgccggctcg accgtcttcg tggccagcag
     1081 caggcgcacc gaatccggct cgcgcccggc ccgctcgcag gcggccgcca tcctctcgcg
     1141 gacccgggcg acgttcgagc ggatcagctc ggcatcagcc ggatcgacgc ttcccgggtc
     1201 caagtcgatc agtccatctc ccggatctcg gtgaagacgg cggtcggggt accgggagcc
     1261 gaatcgacgc gccgttcgtc ggcgatcacg tgggacacgg cgttgatcag ggccagatgc
     1321 gtgaaagcct ggggaaagtt tccgaggtgg cggttgctgg aagcctccag ttcctcggcg
     1381 tagaggtcga gatcgccaga ggcggccaac aaccgttcgc agagcttctt cgcggcggac
     1441 ttctcgccga tctcggagag cgccgcgacc atccagaagg agcagatcag gaaggttcct
     1501 tcttcgccgt ggagcccgtc gtcggtttca tcggtgatgt agcggaggat caggccccga
     1561 tcgtcgagcc cgtcccggat cgccaggacc gtgtcgcgga tccgcgggtc atccgggggc
     1621 aggaagcgga ccaggggcat cagcaggacc gaggcgtcga gcgcgtcggt ctcgtaatgc
     1681 tggcggaaga cgccgtccct gcaacccttt tcgatgatct cttccttgat ttcggccgcc
     1741 ttcgcgcgca gccggtcggc gttctccttg accccgaact cggcggcaag gcgcgaggcc
     1801 cgctccactg ccacccagat catcagtttc gacgagacgt agtgcctggg ttcgccgcgg
     1861 gcttcccaga tgccctgatc cggttttggc caggcctcga gcgcgttctc gaccagccgc
     1921 ctgaccaggt cccgggtctc gtcattgatc gctccgggcc cgagcgcctt gcgatgaacg
     1981 tagatcgaat cgagcagggc gccccagaca tcgttctgac gctggtcgaa ggcgccattt
     2041 ccggtccgga ccggcttcgc accgccgtat ccggacagat ggtcgagcgt gtgctcggtc
     2101 aggtcgtgct cgccgccgat cccgtacatg atctgcaggt cgcctccgga ctccatcgcc
     2161 cggtcgcgca cgaatcgcat gaagtcccgg gcttcctgtt cgaatcccag cgagtgcatc
     2221 gaccagagcg cgaaggtgga atcacggatc caggagtagc ggtagtccca gttccgttcg
     2281 ccgccggggg tttccggcag tgaagtggtg gccgccgcca gcatcgcgcc ggacggggtg
     2341 taggtgagtc ccttcagaac cagggcagaa cgctggaggt ggatccgcca gggatggtcg
     2401 ggaaaggtgc cctgggcaag ccagtcccgc cagaggtcgc gtgaggactc gatcctttcc
     2461 gtggcttccg ggtagctgcg cggatggggg ccgatgccct cgccccagct gaggcagacg
     2521 aaggcgctgt cgccatgctt catcttcagc cgtccggtcg cctgcc
//
LOCUS       DAOQMF010000218         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24613_length_2562_cov_3.863183, whole genome shotgun sequence.
ACCESSION   DAOQMF010000218 DAOQMF010000000
VERSION     DAOQMF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24613_length_2562_cov_3.863183"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="miaB"
                     /locus_tag="PLV77_04610"
     CDS             <1..952
                     /gene="miaB"
                     /locus_tag="PLV77_04610"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNC15158.1"
                     /translation="EFEDFAGHLPSHRARQFQGWLQISQGCNCVCSYCIVPSTRGREQ
                     SRDPRELLKEAERLAADGVREITLLGQNVNSYGRDLPKESRIRFSDLLGMIDAVDGID
                     RLRYTSPHPKDMREDVIEAHASLPSLCEHIHLPLQSGSSSILKKMRRTYTRERYMDRV
                     AMIREQVPDCAITTDIIVGFPGETEEDFAETLEVVEEVGYDGAFTFVYSPRRGTLAAE
                     MEDQIAHPVKVERMERLVEAVQRRARERAQRFVGRNMEVLVEGPSRNDPTRIRGRSRH
                     NKAVNFEGTAEAGQMVEVEIGAATSQTLTGTERLLSGIGA"
     gene            963..1946
                     /gene="miaA"
                     /locus_tag="PLV77_04615"
     CDS             963..1946
                     /gene="miaA"
                     /locus_tag="PLV77_04615"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNC15159.1"
                     /translation="MAVDPRPGKPPSADPPKAGQPSVLPVIAIFGPTAIGKTGVAIEL
                     AELLRRKGEDPVAINCDSIQVYRGLELISGAASPEDQTRLEHRLLSFVPIDQEFSAGR
                     FGEAARGEIDSALEAGRRPILVGGTGLYLRSALSDLEMRPPVDQELRRQVELEVESRG
                     PAALHAELPPGLSARVHPNDRKRVARLTELSRSGVDPHPDHEGGGELWTSRLRHPTLL
                     VGLVEDDEPLVEKIHRRVGRMAGAGAGAEAERAVANGASRTVRAAIGFDEFQNDDLER
                     VAALHRRYGRRQMTWMRRMEGVQLLDRTGRSDSGVAAEILELAEQVPSRVA"
     gene            1943..>2562
                     /gene="dapF"
                     /locus_tag="PLV77_04620"
     CDS             1943..>2562
                     /gene="dapF"
                     /locus_tag="PLV77_04620"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNC15160.1"
                     /translation="MKFEKWQALGNDYVIIEAADLPWEPTQARIQRICDPHFGIGADG
                     VLLISKPQDPKYVADLRIFNPDGSEAELSGNGAREAILYLRHHGWTGEDTFSIMTVAG
                     PVTPTITSDRTCSVEMGRATTTSKDYPDGPADGLGTLASGDRDWKFQHVSIGNPQCAI
                     EAGADLEELDLAKIGPGIENNVLFPNRTNVSFYRVSGSRVRARIFER"
BASE COUNT          472 a          828 c          876 g          386 t
ORIGIN      
        1 cgaattcgag gatttcgccg gtcacctgcc ctcgcaccgg gcccggcagt ttcagggctg
       61 gctgcagatc tcccagggct gcaactgcgt ctgctcgtac tgcatcgtgc cttcgaccag
      121 ggggcgggaa cagagccgcg acccccggga gctgctcaag gaagccgaac ggctggcggc
      181 tgatggcgtg cgggaaatca ccctgctggg ccagaacgtc aactcctacg gccgcgacct
      241 gccgaaggaa agccggatcc ggttttccga cctgctcggc atgatcgacg cggtcgatgg
      301 gattgaccgg ctcaggtaca ccagtccgca tccaaaggac atgcgcgaag acgtgatcga
      361 ggctcacgcg agcctgccct cgctgtgtga gcacatccac ctgccgcttc agtcggggtc
      421 cagttcgatc ctgaagaaga tgcgtcgcac ctacacgcgc gagcggtaca tggaccgcgt
      481 cgcgatgatc cgggagcagg ttccggactg cgcgatcacc accgacatca tcgtcgggtt
      541 ccccggggaa accgaggaag acttcgcgga gacccttgaa gtggtggagg aggtcgggta
      601 cgacggcgcc ttcaccttcg tgtactcgcc ccgtcgcggc accctggccg ccgagatgga
      661 agaccagatt gcccatccgg tcaaggtcga acggatggaa cggctggtcg aggcggtcca
      721 gcgccgggcc agggagcggg cgcagaggtt cgtcggccgc aacatggaag tgctggtcga
      781 gggcccgagc cgcaacgatc cgacccggat ccgtggccgc agccgtcaca acaaggcggt
      841 caacttcgaa ggcaccgccg aagcgggcca gatggtcgaa gtggagatcg gcgccgccac
      901 ctcacagacc ctgaccggca ccgagcgcct gctcagcggc atcggcgcct gaggagagcc
      961 ggatggcggt tgatccgcga ccgggaaagc cgccttcggc agatccgccg aaggccgggc
     1021 agccatcggt cctgccggtg atcgcgatct tcggcccgac cgcgatcggg aagaccggag
     1081 tggcgatcga acttgccgaa ctgcttcggc gaaaggggga ggacccggtc gcgatcaact
     1141 gcgactcgat tcaggtttac cggggcctgg agttgatcag cggcgccgcc tcgccggaag
     1201 accagaccag gctcgagcac cggctgctct ctttcgttcc gatcgatcag gagttcagcg
     1261 ccgggcggtt cggcgaagcc gcccggggcg agatcgactc ggccctggag gcggggcgcc
     1321 gcccgatcct ggtcggggga accgggctct atctgcgctc ggccctatcc gacctggaga
     1381 tgcggccccc ggtcgatcag gaacttcgcc ggcaggtcga actcgaagtc gagtcccggg
     1441 ggccggcagc gctccatgcc gaactcccgc ccgggctttc ggcccgggtc catcccaacg
     1501 accgcaagcg ggtggcgcgg ctgaccgaac tgagccgttc cggcgtcgac ccccacccgg
     1561 atcacgaggg aggcggcgaa ctgtggacga gccggcttag acacccgacc ctgctggtcg
     1621 gcctggtcga agacgacgag ccgctggtcg agaagatcca tcggagggtt ggccggatgg
     1681 ccggggcagg agccggtgcc gaggccgaac gggcggtcgc caacggggca tcccggacgg
     1741 tccgggcggc gatcggcttc gacgagttcc agaacgatga tctcgagcgg gtggccgcac
     1801 tccaccgcag gtacggacgg cgccagatga catggatgcg ccgaatggag ggggttcagc
     1861 tgctcgaccg gaccggcaga agcgattccg gggtggcggc agagatcctc gaacttgccg
     1921 aacaggtacc ttcccgggtc gcatgaagtt cgaaaagtgg caagcgctcg gcaacgacta
     1981 cgtgatcatc gaggccgccg atctgccctg ggagccgacc caggcccgga tccagagaat
     2041 ctgcgatccc catttcggga tcggagccga cggggtgctg ctgatctcga aaccgcagga
     2101 tccgaagtac gtcgccgatc tgaggatctt caacccggac gggtccgagg ccgagctttc
     2161 gggaaacgga gcccgtgaag cgatcctcta cctgcgacac cacggctgga ccggcgagga
     2221 cactttcagc atcatgaccg tcgccggtcc ggtcaccccg acgatcactt ccgatcgcac
     2281 ctgctcggtt gaaatgggcc gggcgacgac cacttcgaag gattatccgg acggaccggc
     2341 ggacgggctt ggcacgctcg cttcgggcga ccgggactgg aagttccagc acgtctcgat
     2401 cggcaacccg caatgcgcga tcgaagccgg ggccgacctc gaggaactcg acctggcgaa
     2461 gatcggcccg ggaatcgaaa acaacgtgct ctttccgaac cggaccaacg tctcgttcta
     2521 tcgggtctcc ggttcccggg tgcgcgcccg gattttcgaa cg
//
LOCUS       DAOQMF010000219         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24694_length_2557_cov_3.781375, whole genome shotgun sequence.
ACCESSION   DAOQMF010000219 DAOQMF010000000
VERSION     DAOQMF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24694_length_2557_cov_3.781375"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PLV77_04625"
     CDS             complement(<1..1430)
                     /locus_tag="PLV77_04625"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNC15161.1"
                     /translation="MSRLATTAAGLFILVLGVAVTSAAEAKEIAAYPSPNVRVASDTT
                     TISFRGIKPKALGRIIVRGSRSGGHKGRLRVHSDGRGVSFIPRKKFDPKEKVTVISKR
                     NTFFGTGKRSKHRYSFVTGLLQPKGWRDEPQVPPQGERPPEWTTYKSFPLLVPKITIN
                     TSKPGVANGDIFMAPRTNGPMIVDNDGGLVYYRPGQRVTDFRTQTFRGKPVLTWWRRA
                     QVGKHVESNYAIAGTDYKVIKRFEAGNGYTSDPHEFTMASDTTAYVNSFRNVIMDLSK
                     YGGLKRTPVMDNVAQEVDLLTGQVIWEWHSLGNIRVPETYKDIPSKITRPFDYFHLNS
                     ISRDSDGNMLISARHTCALYKINRETGKVMWRMGGKDSDFKLASGFRFCYQHDFRPHP
                     NGTYSIFENGAADGELKPKARMSKAYIFRINQKKKTTALVKTYIHPGNLLSPSQGNTQ
                     LLPNGNVFTGWGSLEYCTEFAPDGEVL"
     gene            1539..>2557
                     /locus_tag="PLV77_04630"
     CDS             1539..>2557
                     /locus_tag="PLV77_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC15162.1"
                     /translation="MSEPPKPRPSGRCGDLSPVSLQWLEQADPVSRKARGQYLTPRPV
                     AEALIDRVGLQPGLRVLDPGVGTGELLRALLDRGPDLEVTGWDTDPTAIRAAGELVPE
                     ARLKLRSALDPDAGSSTPAGSSGPDGTETGGFDLVIGNPPYFQLRLDPEERKRFAGVI
                     SGRPNIFALFFQIGLQELKPGGTLAFIVPPSMNSGAYFEALREYIAARAKITDLTILE
                     GTGLFEGANTAIQLLVLRKNGPEEPVGRISGTGDGNPTHRGFIYRREVEAAGFRRVLF
                     GPDPGRLAAEFEGRSSLWELGLEATTGQVIWNENRARLFPDPGPGRVPLIWSRSIGEG
                     ELRTLP"
BASE COUNT          403 a          789 c          873 g          492 t
ORIGIN      
        1 agcacctcgc cgtcgggggc gaactcggtg cagtactcga gcgagcccca gccggtgaag
       61 acgttgccgt tcggcaggag ttgcgtgttg ccctggctcg gtgagagcag gttgccgggg
      121 tggatgtagg tcttgaccag ggcggtggtc ttctttttct ggttgatccg gaagatgtag
      181 gccttcgaca tcctggcctt cggcttcagt tcgccatcgg cggcgccgtt ctcgaagatc
      241 gagtaggtgc cgttggggtg gggccggaag tcatgctggt aacagaagcg gaaaccgctg
      301 gccagtttga agtccgagtc cttgccgccc attcgccaca tgaccttgcc ggtctcgcgg
      361 ttgatcttgt acagcgcgca ggtgtgacgg gccgagatca gcatgttgcc gtcggaatca
      421 cgggaaatcg agttgaggtg gaagtagtcg aaggggcggg tgatcttgct cgggatgtcc
      481 ttgtaggtct ccggtacccg gatgttgccg agcgaatgcc attcccagat cacctggccg
      541 gtcagcaggt cgacttcctg ggcgacgttg tccatcaccg gggttcgctt gaggcccccg
      601 tacttgctca ggtccatgat cacgttgcgg aaggagttca cataggccgt ggtgtcactg
      661 gccatggtga actcatgcgg gtcgctggtg tagccgttgc cggcttcgaa gcgcttgatc
      721 accttgtagt cggtaccggc gatcgcgtag ttgctctcga cgtgcttgcc gacctgggcg
      781 cgtcgccacc aggtcaggac gggcttgccc ctgaaggtct gggtgcggaa gtcggtgacc
      841 cgctggccgg gccggtagta gaccaggccg ccgtcgttgt cgacgatcat cgggccgttg
      901 gtgcggggcg ccatgaagat gtcaccgttg gccacgccgg gtttgctggt gttgatcgtg
      961 attttcggga ccagcagcgg gaaggacttg taggtggtcc actcgggcgg ccgctcgccc
     1021 tgcggcggaa cctgcggctc atcccgccag cccttgggct ggagcagacc ggtcacaaag
     1081 ctgtagcggt gcttgctgcg cttgccggtg ccgaagaagg tgttccgctt cgagatcacg
     1141 gtcacctttt ccttcgggtc gaacttcttc cgggggatga agctgacgcc ccggccgtcg
     1201 gaatggacgc gaagccggcc cttgtggccg ccgctgcggg agccgcgcac gatgatcctg
     1261 ccaagcgcct tcggtttgat gccgcggaac gagatggtgg tcgtatccga agcgacccgg
     1321 acgttcggac tggggtatgc ggcgatttcc tttgcctcgg cagccgaagt gacggctaca
     1381 ccaagtacaa gaatgaacag gcctgctgcg gtcgtggcta agcgcgacac ctgctccctt
     1441 tctcatcgtg attggcggat ccgcaatctt agaccactca aagcgccgga agttgcgggt
     1501 ggagcggcca ctcttcaggc agatacgatg gcgccgcttt gagtgagccg cccaaaccgc
     1561 gcccctcagg ccgttgcggc gacctctccc cggtcagcct tcagtggctc gaacaggccg
     1621 atccggtttc ccgtaaggcc cggggccagt atttgacccc gcggccggtc gccgaggcgc
     1681 tgatcgaccg ggtcgggctt cagcccggtc tgagagtcct cgatccgggg gtcggcaccg
     1741 gcgagctgct ccgggccctg ctggaccggg gaccggacct cgaggtgacc ggctgggaca
     1801 ccgatccgac cgcgatccgc gccgcaggcg aactggttcc cgaggcgcgt ctgaagctcc
     1861 gctccgcgct cgatccagac gcaggcagct ccaccccggc agggagctcc ggcccggacg
     1921 ggaccgaaac cggtggcttt gacctggtga tcgggaaccc gccctatttc cagcttcgcc
     1981 tcgacccaga ggagaggaag cgtttcgccg gggtgatctc cggccggccc aacatcttcg
     2041 cgctcttctt tcagatcggc cttcaggagc tgaaaccggg gggcacgctc gccttcatcg
     2101 tcccgccgtc aatgaacagc ggcgcctatt tcgaggccct gcgcgaatac atcgcggccc
     2161 gggcgaagat cacggacctg accatcctcg agggaaccgg cctcttcgaa ggcgccaaca
     2221 ccgcgatcca gctcctggtc ctgagaaaga acggccccga agaaccggtg ggtcggattt
     2281 ccggtacagg tgacggaaat ccgacccacc gggggttcat ctaccggcgg gaggttgagg
     2341 cggcgggttt ccggcgggtt ctgttcggtc ccgacccggg ccggctggcg gccgagttcg
     2401 aggggcgaag ctcgctctgg gagcttggcc tcgaagccac caccggccag gtgatctgga
     2461 acgagaaccg ggcccggctg ttcccggacc ccggtccggg ccgggttccg ctgatctggt
     2521 cccgcagcat cggcgaaggg gaactccgga cccttcc
//
LOCUS       DAOQMF010000220         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24796_length_2552_cov_2.959151, whole genome shotgun sequence.
ACCESSION   DAOQMF010000220 DAOQMF010000000
VERSION     DAOQMF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24796_length_2552_cov_2.959151"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLV77_04635"
     CDS             <1..733
                     /locus_tag="PLV77_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC15163.1"
                     /translation="TDFGIARAAEQTRITQVGSVVGTAAYLAPEQVRGEEATPSTDVY
                     ALGVVLYQFLTGRLPYEGSSLAELAVRQQNEKPLPPRTYNADVPETVSAAVMRALEGD
                     KAYRFNTAAALSDGLQRGLRGEDVTLQMDEEPVTPAEFPQAETATRPLERTARTAHRP
                     APPPPRYTLPQTAPRPRQKRSFIRSFFRFVLGILALVLIAAVVAGSVILLTDRAASVK
                     IKEWTGNSVEKISNELKQQIRDNTQ"
     gene            complement(734..1459)
                     /locus_tag="PLV77_04640"
     CDS             complement(734..1459)
                     /locus_tag="PLV77_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15164.1"
                     /translation="MRAELLKLRSMPTPRWTLAILLAFFVGGIVASVIWGVGTDSAVL
                     DLAVGLPGQIAALVVGSWMAGVEFGQNTLRRALSADPRRLRLVLSKLAVLVLAVVAVT
                     VILIVLGTLIFSLAGSGHATSIEIGTAGKILGAWLVADISWAVTAFSLTLLTRSMAGG
                     LVLSFVFFFVLDGFISLIPKVGDYTIGIALSDIDIAIRGTTDDFFGQTTTNDTGIAVI
                     VLVGWLAVFIAAGIVRTLKTEVK"
     gene            complement(1449..2384)
                     /locus_tag="PLV77_04645"
     CDS             complement(1449..2384)
                     /locus_tag="PLV77_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC15165.1"
                     /translation="MPETLIRLEGVTKKFGATVALDDLSLAVPEGSVCGLLGPNGSGK
                     TTAIRILLGLAGMNSGTASLLGARPGGPGFTEAVRQTGSLIEGPALYGRATARQNMQI
                     QAATLGLADAGAEIDDLLELVDLGDRADSNARTFSLGMKQRLGLAISLLGKPRLVLLD
                     EPTNGLDPSGIVEIRELIKQLPERGVTVLVSSHLLSEVQLMCDRVTIINKGRLVADGT
                     MEEILASTGVGEGYRVYVRISQAEAATAILRRAGLEVEDRGEGSLVARGEIEDDSEIT
                     RILAESGIWLRGLMRERPDLERVFLSLTDGAGDES"
BASE COUNT          464 a          880 c          825 g          383 t
ORIGIN      
        1 gaccgatttc gggatcgccc gggcggccga gcagacccgg atcacccagg tcggctccgt
       61 ggtcggaacc gccgcctacc tggcccccga acaggtccgg ggtgaagagg cgacaccgtc
      121 gaccgacgtt tacgccctgg gtgtcgtgct ctaccagttc ttgaccggtc gcctgccata
      181 cgagggttcc tcgctggccg agcttgcggt gcgccagcag aacgagaagc cgctgccgcc
      241 gaggacctac aacgccgacg tgccggaaac cgtaagcgcc gcggtgatga gggcgctcga
      301 aggtgacaag gcctaccgct tcaacaccgc cgcggctctt tccgacgggc tccagcgagg
      361 gttgcggggc gaggacgtga ctctccagat ggacgaggaa ccggtaactc cggccgaatt
      421 cccgcaggcc gagaccgcga cccggccgct ggaacgcacg gcccgcaccg ctcaccggcc
      481 ggccccgccg ccgccccgct acaccctccc ccagaccgct ccccggcccc gccagaagcg
      541 ttcgttcatt cgctctttct tccgcttcgt gctgggcatc ctcgccctgg tcctgatcgc
      601 cgcggtggtc gcgggttcag tcatcctgct gaccgaccgg gcagcctcgg tcaagatcaa
      661 ggaatggacg gggaattcgg tcgagaagat ctcgaacgag ctgaagcagc agatccgcga
      721 caacacccag tagtcatttc acttcggtct tgagggtgcg gacgattccc gccgcgatga
      781 agaccgccag ccagccgacc aggacgatta ccgcgatccc ggtgtcattg gtcgtggttt
      841 gaccgaagaa gtcatcggtg gtgccgcgga tcgcgatgtc gatgtcagac agggcgatgc
      901 cgatcgtgta gtccccgacc ttcggaatca gtgaaatgaa gccgtcgagg acgaagaaga
      961 agacgaagga aaggaccagg cccccggcca tcgaccgggt gaggagggta agcgagaagg
     1021 cggtcaccgc ccaggaaatg tcggcgacca gccaggcgcc gagaatcttc cccgcggtgc
     1081 cgatctcgat cgaggtggca tgccccgagc cggccagact gaaaatcagg gttccgagga
     1141 cgatcaggat taccgtcacc gcgaccacgg ccaggacgag cacggccagc ttcgaaagca
     1201 ccaggcgaag ccgccgcgga tcagccgaga gcgcccgccg aagcgtgttc tgcccgaact
     1261 ccaccccggc catccaggag ccgacgacca gcgccgcgat ctggcccgga agcccaaccg
     1321 caaggtcgag tacggcactg tcggtcccca cgccccagat caccgaagca acgatgccgc
     1381 cgacgaagaa ggcgagcagg atcgccagcg tccagcgcgg tgtcggcatc gaacgcagct
     1441 tgagcagctc agctctcatc gccggctcca tcggtcagcg agaggaagac cctctcgagg
     1501 tcgggacgct cgcgcatcag cccgcgcagc cagatgccgg attcggcgag gatgcgggtg
     1561 atctccgagt cgtcctcgat ctcgccccgg gcgacgaggc tgccctctcc cctgtcctcg
     1621 acttccagac ccgcccggcg gaggatcgcg gtcgccgcct cggcctgcga gatccgcacg
     1681 taaaccctgt agccctcgcc gaccccggtc gaggcgagga tctcctccat ggttccgtca
     1741 gcaaccagcc tgcccttgtt gatgatcgtg acccggtcgc acatcagctg gacctcggag
     1801 agcagatggg aagagaccag cacggtcacg ccgcgctcgg gaagttgctt gatcaattcc
     1861 ctgatctcga cgatccccga agggtcgaga ccgttggtcg gctcatcgag caggaccagc
     1921 cggggcttgc cgagcaggga gatcgccaga ccgaggcgct gcttcatgcc cagcgaaaag
     1981 gtccgggcgt tggagtcggc ccgatcgccc aggtcgacca gttccagcag gtcgtcgatc
     2041 tcggccccgg catccgcgag cccgagcgtc gcggcctgga tctgcatgtt ctgccgggcg
     2101 gtggcacggc cgtagagcgc cgggccctcg atcagcgagc ccgtctggcg aaccgcttcg
     2161 gtgaaaccag ggccgccggg ccgggctccg agcaggctcg cggttccgct gttcatcccg
     2221 gccaggccga gcaggatcct gatcgcggtc gtcttgccgg aaccgtttgg accgagcagc
     2281 ccgcagaccg acccctcggg caccgcaagg gagaggtcgt ccagggcgac ggtggccccg
     2341 aacttcttgg tcactccttc cagccggatt agcgtctcgg gcatcgcggg cggaatccta
     2401 accggcttcc cagggcgcca gaccgcccag gatccgctca ctgtaggcag cgtaggttcc
     2461 ggccgaaatc gcctcgcggg caccacgcat cagccgttcc atgtaggtca ggttgtggtc
     2521 gaccagcagc cggacggcgg tcagctcttc cg
//
LOCUS       DAOQMF010000221         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24812_length_2551_cov_3.412260, whole genome shotgun sequence.
ACCESSION   DAOQMF010000221 DAOQMF010000000
VERSION     DAOQMF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24812_length_2551_cov_3.412260"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLV77_04650"
     CDS             complement(<1..784)
                     /locus_tag="PLV77_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC15166.1"
                     /translation="MKVIIQIPCLNEEETLPATLADLPREIEGVDEIETLVIDDGSTD
                     RTIEVARQCGVDHIVKLPNNRGLATGFQSGLDACLKLGADIVVNTDADNQYSGSDIPK
                     LVAPILAGDAEMVVGDRKVAEIDHFSPAKKVLQKFGSWVVRQLSNTEVTDTTSGFRAY
                     NREAALQLLVVDNFTYTLESLIQAGKGNVAVGEVTISTNEKTRESRLFGSTSAYVRRN
                     GLAILRIYTRYEPLKVFSLLGLIAAVAAIAAWMPFLIDWIVNG"
     gene            803..1933
                     /gene="ribD"
                     /locus_tag="PLV77_04655"
     CDS             803..1933
                     /gene="ribD"
                     /locus_tag="PLV77_04655"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNC15167.1"
                     /translation="MPAAQPTSEADYWQRAIQLAEHGLGWTSPNPAVGAVLVKDGRVI
                     GEGWHRRHGELHAEREALADAERRGNDPAGATIFVTLEPCAHTGSQPPCADGLIEAGV
                     GEVVIAAEDPTEKTRGVGPARLEDAGIRVRWAEGPAADAALALIQDFRKRALTGKPLV
                     TLKMAMSLDGKVATRTGDSKWISGEESRALVHRWRAEMDAVAVGSGTLTADDPRLTAR
                     HTDGEPVRQPARVIFDSGLLITTGAALFADIDEAPLLIIAGPEPDPDRLAALKAAGAE
                     VIPVAGEGSGARFALAMEALGERGIGSLLLEGGPRLAGAALTAGEVDRTEIFIAPLIL
                     GGGRPATEGSGPELMEGATRVPKMRVSRVGQDVLMSSTIRTW"
     gene            1933..2550
                     /locus_tag="PLV77_04660"
     CDS             1933..2550
                     /locus_tag="PLV77_04660"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNC15168.1"
                     /translation="MFSGLVQDLGEVKSIDLSSEGARISVATTLAGEIGLGDSVAVNG
                     CCLTATAVDAGGFTADAMQQTLDLTSIGGLEAGSPVNLELALKASDRLGGHIVQGHVD
                     GTAAVIGREQDGFSLRLRISLPAELSRYVIPQGSITIEGVSLTVADCGEDWAEVALIP
                     ETRERTNLGTLQIGTTVNIECDVIAKYVERMVSPYAPGSESHTDR"
BASE COUNT          438 a          819 c          875 g          419 t
ORIGIN      
        1 cgccgttcac gatccagtcg atcaggaacg gcatccaggc cgcgatggcg gctacggcgg
       61 cgatcaagcc gagcagcgaa aagaccttga gcggctcgta gcgggtgtag atccggagga
      121 tcgccaggcc gttgcggcgc acataggcgg aagtcgaacc gaacaggcgg gactcgcggg
      181 tcttctcgtt ggtcgagatc gtgacctcgc cgaccgcgac gttccccttg ccggcctgga
      241 tcaggctttc gagcgtgtag gtgaagttgt cgacgaccag cagctgaagc gccgcttccc
      301 ggttgtaggc gcgaaaaccg gaagtcgtat cggtgacctc ggtgttggaa agctggcgca
      361 cgacccagct gccgaacttc tggagaacct tcttggccgg tgagaagtga tcgatctcgg
      421 ccactttgcg gtccccgacc accatctcgg catcaccggc caggatcggc gcgaccagct
      481 tcggaatgtc gcttccggag tactggttgt cggcgtcggt gttgacgacg atgtcggccc
      541 cgagcttgag gcaggcatca agtccggact ggaaaccggt tgccagaccg cggttgttgg
      601 gcagcttgac gatgtggtcg acgccgcatt gccgggcgac ttcgatcgtc cggtcggttg
      661 agccgtcatc gatgaccaag gtctcgattt catccacgcc ttcgatctcg cgcggcaggt
      721 ccgcgagagt tgcaggcagg gtctcctcct cgttgaggca ggggatctgg atgattacct
      781 tcattaggct gaggattctc ccatgcctgc cgctcaaccc acctccgagg ccgactactg
      841 gcaaagggcc atccaactgg ccgaacacgg cctcggctgg accagcccca atccggcggt
      901 cggtgcggtg ctggtcaagg acggccgggt gatcggtgaa ggctggcatc gccgccacgg
      961 ggagctccac gccgaacggg aggcattggc cgatgcggaa aggcgcggca acgacccggc
     1021 cggcgccacg atcttcgtga ccctggaacc ctgcgcccac accggcagcc agccgccctg
     1081 cgccgacggg ctgattgagg ccggggtagg cgaggtggtg atcgccgccg aggacccgac
     1141 cgaaaagacc cggggcgttg gcccggcccg gctcgaagac gccgggatcc gggtccgctg
     1201 ggccgaaggg ccggctgccg acgcggcgct cgccctgatt caggactttc gcaagcgggc
     1261 cctgaccggc aaacccctgg ttaccttgaa gatggcgatg agcctcgacg gcaaggtcgc
     1321 gacccggacc ggggactcaa aatggatttc cggcgaagag agccgggcac tcgttcaccg
     1381 ctggcgggcc gagatggacg cggtggcggt cggctccggc accttgaccg ccgacgaccc
     1441 gagactgacc gcgcgccaca ccgacggcga accggtccgg cagccggcga gggtgatctt
     1501 tgactctggg ctgctcatca cgaccggggc agccctcttc gccgatatcg acgaggcgcc
     1561 gctcttgatc attgccggtc ccgagccgga ccccgaccgg ctggccgcgc tgaaagcggc
     1621 cggagccgaa gtgatcccgg tcgccggtga aggctccggg gcccggtttg ccctggcgat
     1681 ggaggcgctg ggcgagcgag ggatcggttc gctcctgctg gaaggtggcc cgcgtctcgc
     1741 cggggcggca ctcaccgccg gtgaagtcga ccggaccgag atcttcatcg cgcccctgat
     1801 tctcggcggc ggacgcccgg ctaccgaggg ttccggaccg gaactgatgg aaggtgcgac
     1861 ccgagtgccg aagatgcgcg tgagccgggt cgggcaagat gtgctcatgt cttcgacgat
     1921 caggacctgg tgatgttctc cggactggtg caggacctcg gtgaggtcaa gtccatcgac
     1981 ctttcatccg aaggggcccg gatcagcgtc gccaccacgc tcgccgggga gatcggcctc
     2041 ggcgattcgg tcgccgtcaa cggctgctgc ctgaccgcga ctgcggtcga tgcgggcggc
     2101 ttcaccgccg acgcgatgca gcagacgctt gacctgactt cgatcggcgg gctcgaagcc
     2161 ggttcaccgg tgaatctcga actggccctc aaggcgagcg accggctcgg cggccacatc
     2221 gtccagggtc acgtcgacgg caccgcggcg gtgatcggcc gcgagcagga cggcttctct
     2281 ctgcgactcc ggatttcact gccggcggaa ctttcgcgtt acgtgatccc gcagggttcg
     2341 atcacgatcg agggcgtcag cctcaccgtc gccgattgcg gcgaggactg ggccgaggtg
     2401 gcgctgatcc ccgaaacccg ggagcgcacc aacctcggaa cgcttcagat cggcacaacc
     2461 gtcaacatcg agtgtgacgt aatcgccaaa tacgtcgagc gcatggtgtc accatatgca
     2521 ccagggtccg aaagccacac ggaccgttga g
//
LOCUS       DAOQMF010000222         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_24912_length_2544_cov_3.769787, whole genome shotgun sequence.
ACCESSION   DAOQMF010000222 DAOQMF010000000
VERSION     DAOQMF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24912_length_2544_cov_3.769787"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLV77_04665"
     CDS             complement(<1..657)
                     /locus_tag="PLV77_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC15169.1"
                     /translation="MKRRPIFTEVLLLNAVMVALAAAAASLVTGLSADHLEKLILIAV
                     AAAAITCLLNVAILRRRFTPLEAVITEMEKVDLSQPGANLPAWIDGVAETEEAERLEL
                     AFLRMMRRLEAERRRSSNAALAAQEEERARVARDLHDQVNQSLTGVLLRLEATRSEAP
                     EELAPEIEETSRLAHQAMDELLTVARQLRPTALDDLGLRAAIAGLVEQLDRPGLTATF
                     T"
     gene            798..1661
                     /locus_tag="PLV77_04670"
     CDS             798..1661
                     /locus_tag="PLV77_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNC15170.1"
                     /translation="MTTTKQSTWGATLRTTILLATLSGLLVVIGFTIGGQQTALLFLG
                     IAVLMNFFSYFFSDKLALKMSGAQPMEESENPEYFQMVRELCQRAEIPMPRLYVIPQE
                     QPNAFATGRNYNHSAVAVTAGITRLLSTDELRGVVAHELAHVKHRDILIQSVASTIGA
                     AITYIAYMLMWFGGDNNSPLGLIGSLAMVLLAPLAASIIQLAVSRQREYAADAGGAAI
                     CGNPESLASALLRLEQGAENIPMKGHVSEASEPLYIVKPFKAKGLAGLFSTHPPIEER
                     VKRLRQMRPSL"
     gene            1771..2118
                     /locus_tag="PLV77_04675"
     CDS             1771..2118
                     /locus_tag="PLV77_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNC15171.1"
                     /translation="MAEIRVKRIYEDSSPEDGRRVLVDRLWPRGVKKDRIDEWAKELA
                     PSNELRREFHDSDLDFDEFRARYMVELEGHEDEMRQLLGSTDGPLTLLFGSRNLEQNN
                     ATVLAEALGDLTG"
     gene            2252..>2544
                     /locus_tag="PLV77_04680"
     CDS             2252..>2544
                     /locus_tag="PLV77_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC15172.1"
                     /translation="MAPEATEQQTIEEITPQEAFAELDGGGVKLIDTREPYEYEESHI
                     EGGELIPPGVIADRIEAAVPDKDQRIILQCRSGARSLVAAEALAGLGYTNLAN"
BASE COUNT          442 a          824 c          844 g          434 t
ORIGIN      
        1 ggtgaaggtg gcggtaaggc cggggcggtc gagctgttcg accaggccgg cgatcgcggc
       61 gcgaagaccg aggtcatcca gcgcggtcgg ccgcagctgg cgggccacgg tcagaagctc
      121 atccatcgcc tgatgcgcga gccggctggt ctcctcgatt tccggcgcca actcctcagg
      181 ggcctcggag cgggtcgcct cgagcctcag cagcacgccg gtcagcgact ggttgacctg
      241 gtcgtggagg tcgcgggcga cccgggcccg ttcttcctcc tgggcggcaa gcgcggcatt
      301 cgagctgcgg cggcgctcgg cctcaaggcg acgcatcatc ctcaggaagg cgagctcgag
      361 ccgttccgcc tcttcggtct cggcgacgcc gtcgatccag gcggggaggt tggcgcccgg
      421 ctggctcagg tcgaccttct ccatttcggt gatcaccgcc tcgagcggtg tgaagcgacg
      481 gcgcaggatt gcgacgttga gcaggcaggt gatcgcggcg gcggccacgg cgatcaggat
      541 cagcttctcc agatgatcag ctgaaaggcc ggtgaccagg ctcgcggccg ccgcggcgag
      601 cgcgaccatg accgcgttca gcagcagcac ctcggtgaag atcggacgac gcttcatctg
      661 gagctgccat ggtcgcaggg tgcgcggccg gtttccggcc agaggccggc ggcgagtggc
      721 tgtcccggtg gtccgccggt caaatggggg acatccccta tggacggaat cggccttccg
      781 cgcaagaatt ccggcctatg acaaccacca agcagtcaac ctggggcgcg acgctccgca
      841 cgaccatcct gctggccacg ctctccggcc tgctcgtggt catcggcttc acgatcggcg
      901 gccagcagac ggccctgctc ttcctcggca tcgcggtcct gatgaacttc ttctcttatt
      961 tcttcagcga caagctcgcc ctgaagatga gcggcgccca gccgatggag gaatccgaga
     1021 acccggagta cttccagatg gtccgtgagc tctgtcagcg ggccgaaatc ccgatgcccc
     1081 gcctttacgt cattccgcag gagcagccga acgctttcgc gaccggccgt aactacaacc
     1141 actccgccgt cgcggtgacg gcaggcatca ccaggctgct ttccaccgat gagctgcgcg
     1201 gcgtggtcgc ccacgaactc gcgcacgtca agcaccgcga catcctgatc cagtcggtcg
     1261 cctcgacgat cggcgccgcg atcacctaca tcgcctacat gctgatgtgg ttcggcggcg
     1321 acaacaattc accgctcggc ctgatcggct ctctggcgat ggtgctgctg gcgccgctcg
     1381 ccgcttcgat catccagctg gccgtttcgc gccagcgcga atacgcagcc gacgccggcg
     1441 gcgccgcgat ctgcggcaac ccggagtccc tggcctcggc gctgctcagg cttgaacagg
     1501 gtgccgagaa catcccgatg aagggtcacg tgagcgaggc ctccgagccg ctctacatcg
     1561 tcaagccctt caaggcgaag ggtctggccg gtcttttctc gacccatccc ccgatcgagg
     1621 aacgggtcaa gcggctccgc cagatgcggc cctcgctgta agggtttcgc aggccggatt
     1681 ttcctcccga agcgcagctg gcagtggggc ccccggatca aatggtccgg gggccttccc
     1741 ttcggggcga cgccgggata gcttgggtcc atggccgaga tcagggtcaa gcggatctac
     1801 gaggattcaa gcccggagga cggccggcgg gtgctggtcg accggctctg gccgcgggga
     1861 gtgaagaagg accggatcga cgagtgggcg aaggagctgg ccccgagcaa tgagttgcgc
     1921 cgggagtttc acgacagcga tttagacttc gatgagttcc gggcccggta catggtcgaa
     1981 ctcgagggcc acgaggacga aatgcggcaa ttgctcggct cgaccgacgg accgttgacg
     2041 ctgctattcg gttccaggaa cctcgaacag aacaacgcga cggtattggc agaagccctc
     2101 ggcgacctga ctggctgacc ggcccggtcg ggcgcttttc ccgatgacct gtacccttgg
     2161 tggtcgcggc tgaggggctg gaaaagcatt ccggacctcg ctatactttt tgaagtaacc
     2221 aaattcagat cgagattgag ggtttttgtc gatggcaccg gaagcgaccg aacagcagac
     2281 aatcgaagag atcacgcccc aggaggcttt cgccgagctc gatggtggcg gcgtcaagct
     2341 gatcgacacc cgcgagccct acgagtacga ggagtctcac atcgagggcg gcgagttgat
     2401 cccgcccgga gtgatcgccg accggatcga agcggccgtg cccgacaagg accagcggat
     2461 catcctgcag tgccgctccg gtgcccgctc cctggtcgcg gcagaagctc tggcggggct
     2521 cggctacacg aacctcgcca acat
//
LOCUS       DAOQMF010000223         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25153_length_2531_cov_3.939015, whole genome shotgun sequence.
ACCESSION   DAOQMF010000223 DAOQMF010000000
VERSION     DAOQMF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25153_length_2531_cov_3.939015"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1777
                     /gene="serA"
                     /locus_tag="PLV77_04685"
     CDS             185..1777
                     /gene="serA"
                     /locus_tag="PLV77_04685"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNC15173.1"
                     /translation="MSERAKVLVKEKIADSGVEQLKENFDVEIGVDMDDAELAERIGE
                     FDAILIRSATKLTPELIEKADNMKVIGRAGTGVDNVDIPAATKRGIIVANAPESNSVA
                     AAEHTMALALALFRNVPQAHSALVDGRWDRAKYKGAELYGKTLGVIGFGRIGQLVAKF
                     AQGFEMEVLAFDKFVSHERFRDLGVEGVDSAEEIYRRADLITIHLPKTPETVDWIDAE
                     AIARFKPGMRIVNCARGELVNLDALIDGLESGQLAGAALDVFPEEPFTEHPIFERDDV
                     VVTPHLGASTAEAQDRAGTDTAEQVTAALTGGVVTNAVNIPAVSPAAMEAVAPYVPLC
                     ETLGKVAVGLTPGSIDRVEVEFRGGIAENDTRLLGIAVQAGLLSGHTEEAVNLVNAPQ
                     MAEERGIEVIEIKDTTADEFTDLVRVTIQSGADVVTVGGTTVGPKNAPYLVSIWGQGF
                     YLPFAEHMAVFRYADQPGMIGKVGSLFGEEGVNIGSAAVGAEADSDNAVMVVTSDAPV
                     SAQTIETIKAVDGFSAGYAIEL"
     gene            1809..>2531
                     /locus_tag="PLV77_04690"
     CDS             1809..>2531
                     /locus_tag="PLV77_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC15174.1"
                     /translation="MRAVVLTGHGGPDDLKVQERPDPPAGPGEVRVAVKAAGVNFADV
                     MATRGLYPDAPKPPCVIGYEFAGEVESVGPGVEGVATGDQVFGMTMFGGYAELVTVPS
                     DDVFPIPDGLSLEQAAALPVNYATALAALVVMGGLSEGQRVLIHAAAGGVGIAATQIA
                     RQAGAEIFGTASAAKHEVIRDQGVEHPIDYRNLDFEQEVRRLTGGEGVDLIIDATGPT
                     NFRKDYRLLRPGGKMVMYGAFEV"
BASE COUNT          448 a          799 c          881 g          403 t
ORIGIN      
        1 gtcgagccct accgcaagct cggccggaac cagttgcgga tcgcgatgtt cccggcggtc
       61 gaccccgctg acgtcgaggc gctcaccgcc tgcgtcgact gggtgatcgg gaacgccggg
      121 gtcaccgcgt gaagagactt gacccacacc ggttctcccg gaccggccaa ccaggaggat
      181 tgatttgagc gagcgagcca aggtactcgt caaggagaag atcgccgaca gcggcgtcga
      241 gcagctgaag gagaacttcg acgtcgagat cggcgtcgac atggatgacg cggaactggc
      301 cgaacggatc ggcgagttcg acgcgatcct gatcaggtcg gccaccaagc tcactcccga
      361 gttgatcgag aaggccgaca acatgaaggt gatcggccgg gccggcaccg gggtcgacaa
      421 cgtcgacatc ccggccgcga ccaagcgggg catcatcgtt gccaacgccc ccgaatcgaa
      481 ttcggtcgcg gcggccgagc acacgatggc tctcgccctg gccctgttcc gcaacgtgcc
      541 gcaggcccat tccgccctcg ttgacggccg ctgggaccgg gcaaagtaca agggcgccga
      601 gctctacggc aagaccctgg gcgtgatcgg cttcggccgg atcggccagt tggtcgccaa
      661 gttcgcgcag ggattcgaga tggaggttct cgccttcgac aagttcgtct cccacgagcg
      721 gtttcgcgat ctcggcgtcg agggggtcga ctcggccgag gagatctacc gccgtgcgga
      781 cctgatcacg atccacctgc cgaagacccc ggagaccgtc gactggattg acgccgaggc
      841 gatcgccagg ttcaagcccg gcatgcggat cgtcaattgt gcccgcggtg agctggtcaa
      901 tctcgatgcg ctgatcgacg gcctcgaatc gggtcagctg gccggggccg ccctggacgt
      961 gttcccggag gagccgttca ccgagcatcc gatcttcgag cgggatgacg tggtcgtgac
     1021 gccgcatctc ggcgcttcga ccgccgaggc ccaggaccgg gccggtaccg acacggccga
     1081 gcaggtgacc gccgccctga ccggcggggt ggtgaccaac gcggtcaaca ttccggctgt
     1141 gagtccggcc gcgatggaag cggttgcgcc ctatgtgccg ctctgcgaga cgctcggaaa
     1201 ggtcgcggtc ggcctgaccc cgggttcgat tgaccgggtc gaggtcgagt tccgcggcgg
     1261 catcgccgag aacgacaccc ggctgctcgg gatcgcggtt caggccggtc ttctttccgg
     1321 ccacaccgag gaggcagtga acctggtcaa cgctccgcag atggccgagg aacgcggcat
     1381 cgaggtgatc gagatcaagg acaccacggc cgacgagttc acggacctgg tccgggtgac
     1441 gatccagtcg ggagccgatg tcgtaaccgt cggaggaacc acggtgggcc cgaagaacgc
     1501 gccttatctc gtttcgatct ggggtcaggg cttctacctg ccgttcgccg agcacatggc
     1561 cgtcttccgc tacgccgacc agccggggat gatcgggaag gtcggatcac tgttcggcga
     1621 agaaggcgtc aacatcggtt cggccgcggt cggtgcggag gcagacagcg acaacgcggt
     1681 gatggtcgtg acctccgatg ccccggtttc ggcgcaaacg atcgaaacga tcaaggcagt
     1741 cgacggtttc tcggccggat acgcgatcga gctctgatca acccggcttc cgcggatagg
     1801 ctggagccat gagagctgtc gtcctgaccg gacacggggg accggatgac ctgaaggttc
     1861 aggagcgtcc ggacccgccg gccggtccgg gcgaggtgcg ggtcgcggtc aaggcggccg
     1921 gagtcaactt cgcggacgtg atggcaaccc ggggtcttta cccggacgcg cccaagccgc
     1981 cctgcgtgat cggatacgag ttcgccgggg aggtcgaatc ggtcgggccc ggcgttgaag
     2041 gggtcgcaac cggagaccag gtattcggca tgaccatgtt cggcggatac gcagaactgg
     2101 tcaccgtccc gtccgatgac gtctttccga ttcccgacgg tttgtcgctg gagcaggcgg
     2161 cggcgctgcc ggtcaactac gcgaccgccc tcgccgctct cgtcgtgatg ggcggcctga
     2221 gcgagggcca gcgggtgctg attcacgcgg cggccggagg cgtcgggatc gccgcgaccc
     2281 agatcgcccg ccaggccgga gccgagatct tcggcaccgc ctctgccgcc aaacacgagg
     2341 tgatccggga ccagggagtg gagcatccga ttgattaccg caacctcgac ttcgaacagg
     2401 aggtccggcg gctgaccggg ggagagggcg tcgatctgat catcgacgcc accggcccga
     2461 ccaacttccg caaggattac cggctgctgc ggcccggcgg caagatggtg atgtacgggg
     2521 ccttcgaagt g
//
LOCUS       DAOQMF010000224         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25189_length_2529_cov_4.064268, whole genome shotgun sequence.
ACCESSION   DAOQMF010000224 DAOQMF010000000
VERSION     DAOQMF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25189_length_2529_cov_4.064268"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLV77_04695"
     CDS             <1..190
                     /locus_tag="PLV77_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC15175.1"
                     /translation="AVLSASRQVGAALGVAILVALLGSAAVVGTADDFDKAWLMIAIS
                     AAIAGATALTIGRAGKTE"
     gene            206..1198
                     /gene="trpS"
                     /locus_tag="PLV77_04700"
     CDS             206..1198
                     /gene="trpS"
                     /locus_tag="PLV77_04700"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNC15176.1"
                     /translation="MRIFSGIQPTGRKHLGNYIGAIRQYVEGQDRGDPAIYSIVDLHA
                     ITVAYEPGTLRTNLYDTTAAILAAGVDPERCILFRQSDIPEHTELCWLLSGVCAHGDL
                     NRMTQFKEKSAQQKDLVSAGIFIYPLLQAADVLAYQADEVPVGEDQRQHIELMREVAR
                     RFNERFGETFTIPEHRIPEIGARVMDLQEPGRKMSTSVGFDSGRVYLLETEKETRKKL
                     GSAVTDSGREVKRGEGKEGVANLIGIYAVATGKTEDEVEAEFEGAGYGDFKKAVADAV
                     VEFLAPVREAYPAIREDEGEIERILADGAAKAREIASVTLADVREAMGVGVPRG"
     gene            1279..2040
                     /locus_tag="PLV77_04705"
     CDS             1279..2040
                     /locus_tag="PLV77_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNC15177.1"
                     /translation="MASRLDLDLDLDLDVFIGPFDLLLSIVLREEISLLEVELGEIVA
                     MYVGKLEEEGELELEVATEFLVLIAALLELKSRLMLPGADEDLTDMEPAEAVEELVAR
                     MLEYRRYRDAGRALKEHFESQRGFLYRSAPLPPELRRVEVETAEKVYEPEQLAAALGD
                     LLEPPPRPDLSHLRATVSLRRRLSVVRSVLMNRPVFDFDDEFGNEDRMTQAVTIFALL
                     DLYKKGEAVWEQTESCGPITVKRIQAGQNGLARTA"
     gene            2037..>2529
                     /gene="scpB"
                     /locus_tag="PLV77_04710"
     CDS             2037..>2529
                     /gene="scpB"
                     /locus_tag="PLV77_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNC15178.1"
                     /translation="MTDLARRIESLLFLSNQPVTEIALAEACEVELEQVQNAVVELEE
                     DLAPGRRGLILRRVGGGLILASDPESEAAARRLLSKPRTPPLTQAQAETLAIVAYLQP
                     VARPEIARIRGVSSESAVNSLSERGLIEESGRSRFGAAIYRTSPLFEKVFGLEGLDAL
                     PDPT"
BASE COUNT          452 a          834 c          837 g          406 t
ORIGIN      
        1 ggcagttctg agcgcttccc gtcaggtcgg cgccgcgctg ggtgtggcca tcctcgtcgc
       61 cctgctcggt tcggccgcgg tcgtcggcac cgccgatgat ttcgacaagg cctggctgat
      121 gatcgcgatc tccgccgcaa tcgccggtgc caccgccctg accataggac gcgccggcaa
      181 gaccgaataa atagtctgcg tcccgatgcg aatcttttcg ggaatccagc cgaccgggcg
      241 caagcacctc ggcaactaca tcggggcgat caggcagtac gtcgagggtc aggaccgcgg
      301 cgacccggcg atctactcga tcgtcgacct gcacgcgatc accgtcgcct atgagcccgg
      361 gaccctgaga accaacctct acgacacgac cgccgcgatt ctcgccgccg gggtcgaccc
      421 ggagcgctgc atcctcttcc gccagtccga catccccgag cacaccgaac tctgctggct
      481 gctctccgga gtctgtgccc acggcgacct caaccggatg acccagttca aggagaagtc
      541 ggcccagcag aaggatctgg tttcggccgg gatcttcatc tacccgctgc ttcaggccgc
      601 cgacgtactc gcctaccagg cagacgaggt accggtcggc gaggaccagc gccagcacat
      661 tgaactgatg cgtgaggtcg cccggcgttt caacgaacgc ttcggcgaaa ccttcacgat
      721 tcccgagcac cggatcccgg agatcggggc gcgggtaatg gacctgcagg agccaggccg
      781 caagatgtcg acctcggtcg gcttcgacag cggtcgcgtc tacctgctcg aaaccgagaa
      841 ggaaacgcgc aagaagctcg gctccgcggt gaccgactcc ggtcgcgagg tgaagcgcgg
      901 tgaaggcaag gaaggggtcg ccaacctgat cggcatctac gcggtggcca ccggcaagac
      961 cgaagacgaa gtcgaagccg aattcgaggg cgccggttac ggcgacttca agaaggccgt
     1021 ggccgatgcg gtcgtcgagt tcctcgctcc ggtccgggag gcctacccgg cgatccgcga
     1081 agacgagggc gagatcgaac ggatcctcgc cgacggggcg gccaaggccc gggaaatcgc
     1141 ttcggtgacc ctggcggacg tgcgcgaggc aatgggcgtc ggcgtgccgc gcggatagcg
     1201 cgaacgaagg catcgaagat ccgcaaatag cgcctttccg gagcggggcc gaccgatcgc
     1261 ccctctaccc tttttgcgat ggcttcccgg ctggacctgg acctcgatct ggatctcgat
     1321 gtcttcatcg ggcccttcga ccttctcctc agcatcgtgc tgcgggagga aatcagcctg
     1381 cttgaagtcg aactcggcga gatcgtcgcc atgtacgtcg ggaagctcga ggaagagggc
     1441 gagctcgaac ttgaagtagc gaccgagttc ctggttctca tcgccgcctt gctggagttg
     1501 aagtcgcggc tgatgctgcc gggggccgat gaggacctga ccgacatgga gccggcagag
     1561 gcggtcgagg aactggtcgc ccggatgctg gaataccggc gctaccggga cgccggccgg
     1621 gccctcaagg agcacttcga aagccagcgg gggttcctct accggtcggc cccgctgccg
     1681 ccggagctgc gtcgggtcga ggtcgagacc gcggagaagg tctatgaacc ggagcagctg
     1741 gcggccgcgc tcggcgatct gctcgaaccg ccgccccggc cggacctctc gcacctgcgc
     1801 gcgaccgtgt ctctgcgccg tcgcctttcg gtcgtccggt cggtcctgat gaaccggccg
     1861 gtcttcgatt tcgatgacga gttcggcaac gaggaccgga tgacccaggc ggtaacgatc
     1921 ttcgccctgc tcgacttgta caagaagggc gaagcggtct gggaacagac cgaatcctgc
     1981 ggaccgatca cggttaaacg gattcaggcc ggacaaaacg gattggcgag gactgcatga
     2041 ccgatcttgc ccgacggatc gaatcgctgc tgttcctcag caaccagccg gtgaccgaga
     2101 tcgccctggc ggaagcctgc gaagtggagc tcgagcaggt ccagaacgcg gtggtcgaac
     2161 tcgaagagga cctggcgccc ggacggcgcg ggctgatcct gcggcgggtc gggggagggc
     2221 tgatcctggc cagtgatccc gagtcggaag cggccgcccg caggctgctc tcgaagcccc
     2281 gcaccccgcc gctcacccag gcccaggccg agacgcttgc gatcgtcgcc tacctgcagc
     2341 cggttgcccg gcctgagatc gcaaggatcc gcggcgtttc ctccgagtcg gcggtcaaca
     2401 gcctcagcga gcgcggcctg atcgaggaat ccggtcgctc ccgcttcggc gccgcgatct
     2461 accggacctc gcccctgttc gagaaggtct tcggactcga gggcctcgac gcgctgccgg
     2521 atccgaccc
//
LOCUS       DAOQMF010000225         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25277_length_2525_cov_3.506478, whole genome shotgun sequence.
ACCESSION   DAOQMF010000225 DAOQMF010000000
VERSION     DAOQMF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25277_length_2525_cov_3.506478"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..753
                     /locus_tag="PLV77_04715"
     CDS             64..753
                     /locus_tag="PLV77_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15179.1"
                     /translation="MNAVRNQSLAIFFGLLFLLALGGQSIAGYQSYNDDEKARAHLAR
                     EAPETIDYPSYLTSPEFSRDVMENWQSEYLQFLLFILATIWLVQRGSPESKRPDESGT
                     ESDRDQKIGPYADPTSPLPARVGGLVTALYSNSLLILMGSIFFAAWFAQSLTGWPAYN
                     EEQVAYGLPTVSWLSYLGTADFWSRTLQNWQSEFLAVGSMAVFAIYLRQRGSPESKPV
                     GESHDSTGIEG"
     gene            778..1626
                     /locus_tag="PLV77_04720"
                     /pseudo
     CDS             778..1626
                     /locus_tag="PLV77_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            complement(1679..>2525)
                     /locus_tag="PLV77_04725"
     CDS             complement(1679..>2525)
                     /locus_tag="PLV77_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15180.1"
                     /translation="MNSHHVESVEILWDEDLALEGRAGYYDRAGALVDMIQSHLLQVA
                     AVLAMEPPPTTGEKDIRDGTARILRAASVWDDDPARFSRRARYSAGTIKGRELPAYAD
                     EEGVEPEKMTETFAEIVIGVDTWRWAGVPFRLRSGKAIGNPRKEAVINFRDPEQIPRG
                     LVGAEGQNRLRIGLGFGSDTVRFDIAVNGPGDPKELDFATLEATLGAGYLSEYGEVLK
                     TVLDGTPALSVRGDAAVDCWRIIDPVRQAWKTGEVPLLEYPAGSSGPEPWPESGLPGS
                     QPGGS"
BASE COUNT          419 a          856 c          813 g          437 t
ORIGIN      
        1 cgtgtatctg atcggggtga ttgcgggcct cgcgtttgcg atctcggtgg gagcaaacag
       61 cgcatgaacg cggtccggaa ccagagcctc gcgatcttct tcggcctgct ctttctgctt
      121 gcgctcggag gccagtcgat cgccggctac cagagctaca acgatgacga gaaggcgcgt
      181 gcccacctgg cgcgcgaggc tcccgaaacc atcgactacc ccagctacct gacctcaccg
      241 gaattctcgc gagacgtgat ggaaaactgg cagtcggaat acctgcagtt cctgctcttc
      301 atcctcgcaa cgatctggct ggtccagcgg ggctcgcctg aatcgaagcg acccgacgaa
      361 agcggcaccg agagtgaccg cgaccagaaa atcggaccgt acgcagaccc gacctctccc
      421 ctccccgccc gggtcggcgg tctcgtcacc gctctctact cgaactcgct actgatcctg
      481 atgggatcga tctttttcgc ggcctggttc gcccagtcac ttaccggctg gcctgcctac
      541 aacgaagaac aggtcgccta cggcctgccc accgtcagct ggttgagcta cctcggaacc
      601 gctgacttct ggagccgcac gctccagaac tggcaatcgg aattcctcgc ggtcggttcg
      661 atggccgtct tcgcgatcta cctgagacag cgaggctcgc cggaatccaa gccggtcggc
      721 gagagccacg attcgaccgg gatcgagggt tgatgcccga tcacctagtt ttcggggatg
      781 ttcggaaacg gagctgaggg tccgcgccag ggcgacgggc agacgcgttc cccgatcagg
      841 gttgctgtcg tcggccacgt tgaatgggcc gagtttgcga tcgtcgacca tgtgccggtg
      901 gccggggaga tcgtccaggc caccgagacc tgggaagagc cggccggagg cggtgccgtc
      961 gccgcggttc aactggcgaa actcaacggc tcctgcctct ttttgaccgc ggtcagcgat
     1021 gacccgctcg gcagccaggt gaagcccgag cttgagcgtc acgggctcga ggtctgcgcc
     1081 gccgttcagg caacgaagca gcgtcgcgcc ttcgttcatc tcgattcccg aggcgagcgg
     1141 acgatcacta cgaccgggga acggctgacg ccgggagcgg gcgacgatct gccttgggca
     1201 cgcctcgcag gcttcgacgc ggtgtacgtg accgcggccg acgccgaggg cttccgcctg
     1261 agccgggccg cgaggaagct cgtcgcgacc atccgggccg cccgcggcct gcttgagtcc
     1321 ggggtcacgg tcgacgtcgt ggtcgcgagc cggaacgacc ccggcgagga cttcgatctc
     1381 gcccagttca cgaatccgcc gggatgtgtg gtgcgaacag acggggcgaa aggcggcacc
     1441 atcgcccact ccgacggaac cgtcgagatc tgggaagccc gcccgctcga gtcaccgtgg
     1501 gtcgacagct acggggcagg tgactcattc gccgccggaa tcacttacgg cctcggtctc
     1561 ggacttccgg ttctcgaggc agcccggatc ggtgcgctct gcggggccgc gaacctgcag
     1621 gggcggggtc cctacgccgg gcaggcgacg gccgaagacc tggcccgttc gatctggctc
     1681 aggacccgcc gggctgagag cccggaaggc cgctctccgg ccagggctcc ggtccgctcg
     1741 agccggccgg gtactcgagc aggggcacct cgcccgtttt ccatgcctgc cggaccgggt
     1801 cgatgatccg ccagcagtcg accgccgcat cgccacgcac cgaaagagcc ggggtgccgt
     1861 caagcacggt cttcagcacc tcgccgtact cggacagata cccggcgcca agcgtcgctt
     1921 cgagggttgc gaagtcgagt tccttcgggt cgccgggacc gttgaccgcg atgtcaaacc
     1981 gcaccgtgtc ggagccgaag ccgagaccga tcctcagacg gttctgaccc tctgcgccga
     2041 ccagccctct cggtatctgt tccggatcgc ggaagttgat gaccgcctct ttccgtggat
     2101 tgccgatggc cttgcccgag cgcaggcgaa agggaacccc ggcccacctc caggtatcga
     2161 caccgatcac gatctcggcg aacgtttcgg tcatcttctc cggctcgacc ccttcctcgt
     2221 ctgcataggc gggaagttcg cggcccttga tcgtccccgc cgagtacctc gcccggcggc
     2281 tgaacctggc cgggtcgtca tcccagaccg aagcggcgcg aaggatcctg gcggtgccgt
     2341 cacggatgtc cttctcgccg gtcgtcggtg gcggttccat cgccagcacc gcagccacct
     2401 ggagcagatg gctttggatc atgtcgacca gcgcaccggc acggtcgtaa tagccggctc
     2461 gcccttccag ggcgaggtcc tcatcccaga ggatctcgac gctctcgacg tgatgggagt
     2521 tcatc
//
LOCUS       DAOQMF010000226         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25472_length_2514_cov_2.760878, whole genome shotgun sequence.
ACCESSION   DAOQMF010000226 DAOQMF010000000
VERSION     DAOQMF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25472_length_2514_cov_2.760878"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PLV77_04730"
     CDS             complement(<1..1368)
                     /locus_tag="PLV77_04730"
                     /inference="COORDINATES: protein motif:HMM:NF020846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HNC15181.1"
                     /translation="MSAFSRFLTLFTLIVLSCGALVTSNAGAANKNAYAMAMLKLHHP
                     AGLQKFARSVSSPNSDNYGQYLPVSRIVKKFGARSKAARRALRYFRSRGMRATLDGAH
                     QNVLVSMDLNQAGSYLAPSASASVARQGGPGSVPPALRGVASSLDFLDPDPEKYSTAG
                     KFPPQPSGSPFPEGSSARPHSGTQAGCAAGQRGATAIGEPNRNFTPNQFLDAYGISDL
                     NQQGFKGQGRRIAVIEIDGFKRSDIEAYGDCYGKRVPPTRVYPVYYKKPLAPGAETTL
                     DLEVITAVAPQLAGIDVYEGPGSEAGFMLMVGRALANQKKPAAISISLGACEPTLWRQ
                     LSYRRALGNLFAVAAGAGISVAVAAGDNGSAECSREGNSSTLPLLTVSDPASLEWVTA
                     VGGTNLELDANNGISQEFVWNDGSLGPSGGGGGFSILATRRPWYQQGTKRFRNYGLTR
                     AVPDVA"
     gene            complement(1405..>2514)
                     /locus_tag="PLV77_04735"
     CDS             complement(1405..>2514)
                     /locus_tag="PLV77_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC15182.1"
                     /translation="NAQRNPSRTAVTAAALMIGLALIAFVTIFASGLSSSVNKVIDDQ
                     LPGEITLQGQGGILPFPAGAIKVVQKVDGVEAASGVRYVSAKVDGKTAAISSVDPADI
                     VKVYTVEWEEGSDSDLTGLTDGQIVIGNKLADSLDVGVGDRVTLTSQKGRKFPFQINA
                     IMKTDSIALAGEGIIDQGAMERDFDQKSDAVGLVKLDPGADLDKTQTAIEDALTAQDF
                     PTVDVLNQGELKEQQKSQIKGLLAMIYVLLALAVIVSLVGIVVTLILSIYERTRELGM
                     LRAVGMSRRQVKRMVRYEAVITAVIGAVSGLIVGVIFAFLIGIPLGGDGFELSYPVGT
                     LIIILILTALAGVIAAIYPARKAAKLDVLEAVSYE"
BASE COUNT          435 a          804 c          834 g          441 t
ORIGIN      
        1 agcgacgtcg ggcacggcgc gggtcagacc gtaattcctg aaccgcttgg tcccctgctg
       61 gtaccagggg cgccgggtgg cgaggatgct gaagccgccc ccgccgccgg acggaccgag
      121 tgaaccgtcg ttccagacga actcctgcga gatgccgttg ttggcgtcaa gttcgaggtt
      181 cgttccgcca acggcggtca cccactcgag gctggccgga tcgctgaccg tgagcagcgg
      241 cagggtcgat gaattcccct cgcgcgagca ctcggcggag ccgttgtccc cggccgccac
      301 cgccaccgag atgccggcac cggccgcgac cgcgaacagg ttgcctagcg cgcggcggta
      361 tgaaagctgt cgccagaggg tcggttcgca ggcgcccagc gagatcgaga tcgccgccgg
      421 cttcttctgg tttgcgaggg cgcggccgac catcagcatg aacccggcct cgctgccggg
      481 cccttcgtaa acgtcgatcc cggcaagctg cggagcgacc gccgtgatca cttcgagatc
      541 gagggtggtt tcggcgccgg gcgccagcgg cttcttgtag tagaccgggt agaccctggt
      601 cggcgggacc cgcttgccgt agcagtcgcc gtaggcctcg atgtcggacc gcttgaagcc
      661 gtcgatctcg atcaccgcaa tccgccggcc ctgacccttg aatccctgct ggttgaggtc
      721 actgatgccg taggcgtcga ggaactggtt gggggtgaag ttgcggttcg gttcaccgat
      781 cgcggtggct ccgcgctgac cggccgcgca gcccgcctgg gttccggaat ggggtcgggc
      841 cgagctgccc tcggggaagg gactgcccga cggctgaggc gggaatttgc ccgcagtcga
      901 gtacttctcc ggatccgggt cgaggaagtc aaggctgctt gccacgcccc gcagggcggg
      961 aggcaccgac cccgggccac cctgacgggc aaccgaggcc gaggcggacg gagcgaggta
     1021 gcttccggcc tggttcaggt ccatgctcac cagtacgttc tgatgcgcac cgtcgagcgt
     1081 ggcccgcatg ccccgggaac ggaagtaacg caaggcccgc ctggcggcct tcgatcgggc
     1141 gccgaacttc ttgacgatcc tgctcaccgg taggtactgc ccgtagttgt cgctattcgg
     1201 actgctgacc gacctcgcga acttctgcaa cccggcggga tggtgaagct tgagcatcgc
     1261 catcgcgtag gcgttcttgt tggcggcacc ggcattcgat gtgaccagcg ccccacatga
     1321 aagaactatc agcgtgaaca aagtgaggaa gcgcgaaaaa gccgacatgg ccggcaagtt
     1381 cccgcctgga ccgcagatca aacgctattc gtacgaaacg gcttccagca cgtcgagctt
     1441 ggccgccttg cgggcggggt agatcgcggc gatcactccg gccagcgccg tgagaatcag
     1501 gatgatgatc agggtgccga ccgggtagct cagttcgaag ccgtcaccgc cgagcgggat
     1561 gccgatcagg aaggcgaaga tgacgccgac gatcaggccg gagacggccc cgatcacggc
     1621 cgtgatcacc gcttcgtagc gaaccatccg cttgacctga cggcgtgaca tcccgaccgc
     1681 cctgagcatg ccgagttcgc gggttcgctc gtagatcgag aggatcagcg tgaccacgat
     1741 gccgaccagc gaaacgatca cggccagcgc gagcagcacg tagatcatcg ccagaagtcc
     1801 cttgatctgg gacttctgct gttccttcag ttcgccctgg ttgagcacgt cgacggtcgg
     1861 gaagtcctga gccgtcaacg cgtcctcgat cgcggtctgg gtcttgtcga gatcggcacc
     1921 ggggtcgagt ttgaccagac cgaccgcatc ggacttctgg tcgaagtccc gctccatcgc
     1981 gccctggtcg atgatgccct cgccggccag cgcgatgctg tcggtcttca tgatcgcgtt
     2041 gatctggaag gggaatttcc ggcccttctg tgaggtcagg gtgactctgt ctcccacccc
     2101 gacatccagc gagtcggcga gcttgttgcc gatcacgatc tggccgtcgg tcagaccggt
     2161 cagatccgaa tcagaacctt cctcccactc gaccgtgtag accttgacga tgtcggccgg
     2221 atcgaccgag gagatcgcgg ccgtcttgcc atcgaccttc gccgatacgt aacggacgcc
     2281 ggaagcggct tccaccccgt cgaccttctg gacaaccttg atcgcgccgg cgggaaacgg
     2341 caggatgccg ccctgaccct gcagggtgat ctcgcccgga agttggtcgt cgatcacctt
     2401 gttcacggac gaactgaggc cggaagcaaa gatcgtgacg aaggcgatca gggcgagccc
     2461 gatcatcagc gccgcggcgg tgaccgcggt gcgtgacggg tttcgctggg cgtt
//
LOCUS       DAOQMF010000227         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25525_length_2511_cov_4.736564, whole genome shotgun sequence.
ACCESSION   DAOQMF010000227 DAOQMF010000000
VERSION     DAOQMF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25525_length_2511_cov_4.736564"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLV77_04740"
     CDS             <1..302
                     /locus_tag="PLV77_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15183.1"
                     /translation="WFFLARAPEGVEVKPDNFEMTEALWVNPSDALTAAEAGDMAIVF
                     PTLKQLEALAPSANTEEAMGRAAADPNASKVVLPKVIGDEQNPRVVLPNEDDYPD"
     gene            complement(309..977)
                     /locus_tag="PLV77_04745"
     CDS             complement(309..977)
                     /locus_tag="PLV77_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15184.1"
                     /translation="MGKQADQWQQIVSSYKGAGEVKWEPVGGINPVGAPVALCVGGSN
                     RLTGELAPDFWGASCDADEEEVGGFMRKELVPTAILAKAHMPDLAQVVPAFNVESIET
                     SELVKQKVSRRKVEFESVDFNKRFLATVPKEHDPIALRELFSPGFLEWTTTIDREVDF
                     GINDRQLWFLWRLRERSAEELKLALANAGELFKRLHSEMDEDGIHTYPGGPWNAGLEP
                     FPAD"
     gene            1061..1627
                     /locus_tag="PLV77_04750"
     CDS             1061..1627
                     /locus_tag="PLV77_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNC15185.1"
                     /translation="MSGALIALIVVVVIALIAVLWYIATRNGIIASRNRVEESWSGID
                     VQLKRRHDLVPNLVETVKGYATHEQKVFEEVTKARADAMKAQSVDETAQAESGLNRSL
                     ADLRAVAEQYPDLKATENFQQLSRNLSELEDEIQASRRIYNSNVQAYNTKIQQFPASV
                     VANQGGFTAKEFFEIEDAAERATPNVSF"
     gene            complement(1687..2388)
                     /locus_tag="PLV77_04755"
     CDS             complement(1687..2388)
                     /locus_tag="PLV77_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC15186.1"
                     /translation="MPTSMERRHNRRRRHERTRQELIEAALNQSAAGSFKDLTVEGVT
                     REAGVARSAFYVYFGDKEELLLGALEDLIASHQNRLGRCWQQGDDPRRDIERGIYGMA
                     RIYAGNSALLALAGEAATYDEEVRELWSTVLETTTEGTREEIERLQKAGSVNESLDPE
                     ALAEGLVLMTERSFQVHLAQGEDDPNEVASSLTRVWWATLFGPPSPPSPPEGEGGSQG
                     SPPGPEGRGMGDPEK"
BASE COUNT          439 a          842 c          795 g          435 t
ORIGIN      
        1 cctggttctt cctcgcccgc gcccccgagg gcgtcgaggt caaacccgac aatttcgaga
       61 tgaccgaggc actctgggtc aatccttccg acgctctcac ggcagccgag gccggggaca
      121 tggcgatcgt cttccccact ctgaaacagc tcgaagcgct tgcgccctcg gcgaacacgg
      181 aggaggcgat gggccgcgct gccgccgacc ccaacgcctc gaaggtcgtc ctgccgaagg
      241 tgatcgggga cgagcagaac ccccgggtcg tcctcccgaa cgaagacgat tacccggact
      301 gactccggtc agtcagccgg aaaaggctca aggccggcgt tccatggtcc gccgggatag
      361 gtgtggatcc cgtcctcgtc catctcgctg tgaagccgtt tgaagagctc gccggcgttg
      421 gcaagggcga gtttcagctc ctccgcagag cgttccctga gtcgccagag aaaccagagc
      481 tgacggtcgt ttatgccgaa gtcgacttcc cggtcgatcg tggtggtcca ctcgagaaag
      541 ccgggcgaga acagctcccg cagcgcgatc gggtcgtgtt ccttcggcac cgtcgccaga
      601 aagcgcttgt tgaagtcgac cgattcgaat tcgaccttgc ggcgggagac cttctgcttg
      661 actagctcgc tggtttcgat cgactcgacg ttgaaggccg ggaccacctg ggccaggtca
      721 ggcatgtggg ccttcgcgag gatcgcggtc gggaccagtt ccttgcgcat gaatccgccg
      781 acttcctcct cgtcggcgtc gcagcttgct ccccagaagt ccggggcgag ctcgccggtc
      841 agccggttgc tgccgccgac acagagtgcg accggtgcgc cgaccgggtt tatgccgcca
      901 accggctccc atttcacctc gcccgccccc ttgtaggaag agacgatctg ctgccactgg
      961 tcagcttgct tgcccatcgc cgatcaccct acccgtagtc acagccgaat caggcggata
     1021 ggatggccgc gatactcatt tcaaccgagg agacttgcct atgagtggcg cacttatcgc
     1081 gctgatagtg gtcgtcgtaa tcgccctgat cgcggtgctc tggtacatcg cgacgcggaa
     1141 cggaatcatc gcttcgcgca accgggtcga ggaatcgtgg agcggcatcg acgtccagct
     1201 gaagcgccga catgacctgg tcccgaacct ggtcgagacg gtcaagggct acgcgactca
     1261 cgagcagaag gtcttcgagg aagtcaccaa ggcccgtgcc gatgcgatga aggcccagtc
     1321 cgtcgacgag accgctcagg cggaatccgg gctcaaccgc tcgctcgccg acctgcgtgc
     1381 cgtggccgag cagtacccgg acctgaaggc gaccgagaac ttccagcagc tcagccggaa
     1441 cctctcggaa ctcgaggacg agatccaggc ttcccgccgg atctacaact cgaacgtcca
     1501 ggcgtacaac acgaagatcc agcagttccc cgcctccgtc gtcgccaatc agggcggctt
     1561 cacggccaag gagttcttcg agatcgagga cgccgccgag cgcgccaccc ccaacgtcag
     1621 cttctgaggg ggcacggcat gggggacccc gaaaagtaac gagcgctccg cgctctgatt
     1681 aaggagtcac ttttcggggt cccccatccc gcgcccttcc ggaccgggcg gggacccctg
     1741 cgagccacct tcgccctccg ggggtgaggg gggcgaaggg gggccgaaga gagtggccca
     1801 ccagactcgg gtcaggctcg aggcgacctc gttcgggtcg tcctcgccct gggcaaggtg
     1861 gacctggaag ctcctttcgg tcatcagcac caggccttcg gcaagtgctt ccgggtccag
     1921 tgattcgttg accgagcctg ccttctgcaa ccgctcgatc tcctcccggg tgccttcggt
     1981 ggtcgtttcc agcacggtcg accagagctc gcgcacctct tcgtcgtagg tggctgcctc
     2041 gccggccagg gcgagcaggg ccgagttgcc ggcgtagatc cgggccattc cgtagatgcc
     2101 ccgttcgatg tcgcggcggg ggtcatcacc ctgctgccag cagcggccga gccggttctg
     2161 gtgggaggcg atcagatcct ccagcgcccc cagcagcagc tcttccttgt cgccgaaata
     2221 gacgtagaac gcggagcggg cgactccggc ctcgcgggtt accccttcga cggtcaggtc
     2281 cttgaacgaa ccggccgccg actggttcag ggcagcctcg atcagctcct gccgggtccg
     2341 ctcatgacgg cgtcgtcggt tatgtcggcg ctccatcgag gtcggcaccc gccaagcata
     2401 tgcaccaccc ccgccggaaa cccgaccggc acgactcgct caggaagagg gcgaggtcgc
     2461 cttgatccgg cggaaggcgg aattggggct gaaccagggc ccgccttccg g
//
LOCUS       DAOQMF010000228         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25827_length_2496_cov_3.290455, whole genome shotgun sequence.
ACCESSION   DAOQMF010000228 DAOQMF010000000
VERSION     DAOQMF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25827_length_2496_cov_3.290455"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLV77_04760"
     CDS             <1..436
                     /locus_tag="PLV77_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15187.1"
                     /translation="GVLIWVTLVYSVVTVAGLFLLVIPGLVIFTIWSVAGQAVVVERK
                     GLFSSLGRSTELISGRGPSVFAFLLLLGIVALSISLLVIVATRPLGDGVLGSLVTPFL
                     TNCLSAPFFVLGVASLYNELSGRGVNPEPEAARPPDPGPDQP"
     gene            complement(381..1982)
                     /locus_tag="PLV77_04765"
     CDS             complement(381..1982)
                     /locus_tag="PLV77_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC15188.1"
                     /translation="MAEQKSGYLVLVAMIFAVSMTFIDQTIVSISIPEIQKDLSLTST
                     GVQWVINGYLLSLAALFALGGKLADVFGHRRLVVIGVLIFAVSSACCGFTPDSSIDEA
                     WLIFFRVIQGAGAALMFPAALAIVISSFPIRDRGKAMAIFFGVTGAFTSIGPLAGGYL
                     SEWTWRAIFWINIPVAILSLIFIWISKPEETKRPQPIDYRGAVLVCIGMALAVLGLQQ
                     SSTWGWGDPVTWICIVGGLLVLAFFIRFEMKQEHPLIRVAIFKDRAFAVDNVLLFLLS
                     VAFVPLFFFASMYAQLALGWETSEAGLYLLIFFAGFATAAQFGGRVLDSRGARPTVIL
                     GSLLASVGFVFWAMKLTDLAGGSNAQWPFIVLTGVGFGLLLGPVATDAVNRAPNTSYG
                     EATGITQTVRNYGASVGMAVLGTILILQNKSNIESELAGKVPARTASQIADEVSKAGS
                     GAVTSSAPSGGKAEEIYHGVQQAFAQSSKTVFLCMAGVMALCFVVALIGSPAGRMEEV
                     FADEGEAPKADPDRDPAAGPPRVQG"
     gene            2060..>2496
                     /locus_tag="PLV77_04770"
     CDS             2060..>2496
                     /locus_tag="PLV77_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC15189.1"
                     /translation="MEIVRTPEERFAQVPDFPWEPVYREWEGMRLAHVDEGEGEPVLL
                     VHGEPTWGFLWRKVMGPLLDAGYRCIVPDLPGFGRSDKPVDDEWYSYDRHTAALVSLI
                     EELDLSGLTLVCHDWGGPIGLRTASTEVPERFSRLVAMDTGLFT"
BASE COUNT          447 a          816 c          827 g          406 t
ORIGIN      
        1 tggcgtgctg atctgggtca cgctcgtcta ctcggtggtg accgtggccg ggctcttcct
       61 gctggtcatc cccggcctcg tgatcttcac catctggtcg gttgccggtc aggccgtcgt
      121 ggtcgaacgg aagggcctct tttcttcgct tggacgcagc acggaactga tcagcggcag
      181 ggggccctcc gtgttcgcgt tccttctctt gctgggcatc gtcgcccttt cgatttcgtt
      241 gctggtgatc gtggcaaccc gcccgctcgg cgacggcgtg ctcgggagcc tggtcacccc
      301 gttcctcacc aactgccttt ccgccccgtt cttcgtgctc ggggtggcct cgctctacaa
      361 cgaattgagc gggcggggcg tcaaccctga acccgaggcg gcccggccgc cggatcccgg
      421 tccggatcag ccttaggcgc ttcaccttcg tcggcgaaga cctcttccat ccggccggcc
      481 ggcgagccga tcagggcgac cacgaaacag agcgccatca ccccggccat gcagaggaag
      541 acggtcttgc tggactgcgc gaaagcctgc tggacgccgt ggtagatctc ctcggccttg
      601 cccccggagg gcgcgctcga ggtgaccgca ccgctgccgg ccttcgagac ctcgtcggcg
      661 atctggctgg ccgtcctggc cgggaccttg ccggccagct ccgactcgat gttggacttg
      721 ttctggagga tcaggatcgt gccgaggacg gccatgccga cactcgcccc gtaattcctg
      781 accgtctggg tgatgccggt cgcctcgccg tagctggtgt tcggcgcgcg gttgaccgca
      841 tcggtggcga ccggcccgag cagcaggccg aagccgacgc cggtcagcac gatgaacggc
      901 cactgcgcgt tggagccacc ggccagatcg gtcagcttca tcgcccagaa gacgaaaccg
      961 accgaggcga gcaggctgcc gagaatcacg gtcggccggg ccccgcgcga gtcgaggacg
     1021 cgaccgccga actgggccgc ggtggcgaaa ccggcgaaga agatcaacag gtagaggccg
     1081 gcttccgagg tctcccagcc gagcgcaagc tgcgcgtaca tgctggcgaa gaagaagagc
     1141 gggacgaagg cgaccgaaag caggaagagc agcacgttgt ccaccgcgaa ggcccggtcc
     1201 ttgaagatcg ccacccggat cagggggtgt tcctgcttca tctcgaagcg gatgaagaag
     1261 gcgaggacga gcaggccgcc gacgatgcag atccaggtga ccggatcacc ccagccccag
     1321 gtcgaagact gctgcagccc gagcacggcc agtgccatgc cgatgcagac gaggaccgcg
     1381 ccccggtaat cgatcggctg cgggcgcttc gtctcctccg gcttcgagat ccagatgaag
     1441 atcagggaaa ggatcgccac gggaatgttg atccagaaga tcgcccgcca ggtccattcc
     1501 gagaggtagc cgccggccag cggaccgatc gaggtgaacg ctcccgtgac gccgaagaag
     1561 atcgccatcg ccttgccgcg gtcccgaatc gggaaggagg agatcacgat cgccagggcg
     1621 gcggggaaca tcagggcggc accggccccc tggatgacgc ggaagaagat cagccaggcc
     1681 tcgtcgatgc tgctgtccgg cgtgaaaccg caacaggccg aggagacggc gaagatcagg
     1741 acgccgatca cgaccagccg gcgatggccg aagacgtcgg cgagcttgcc gccgagcgcg
     1801 aagagcgcgg ccagcgacag cagatagccg ttgatcaccc actgaacccc ggttgaagtg
     1861 aggctcaggt ccttctggat ctcagggatc gagatcgaga cgatcgtctg gtcgatgaag
     1921 gtcatcgaca cggcgaagat cattgcgacc aggacgaggt agccactctt ctgctctgcc
     1981 atctgaccca acttaccgaa gcgacccgtc gctcaaacaa agttgacacg catcagcttc
     2041 taccggatag gctgcccgca tggaaatcgt tcgcacaccc gaagaacgct tcgcgcaggt
     2101 tccggatttc ccctgggagc cggtctaccg ggagtgggag ggcatgcggc tcgcccatgt
     2161 cgacgagggg gaaggcgagc cggtgctgct ggtccacggc gagccgacct ggggttttct
     2221 ctggcgcaag gtgatgggcc ccctgctcga cgccggctac cgctgcatcg tgcctgacct
     2281 gccgggcttc ggacgctccg acaagccggt cgatgacgag tggtacagct acgaccgtca
     2341 caccgccgcc ctggtcagcc tgatcgagga gctcgacctg agtggcctga ccctggtctg
     2401 ccacgactgg ggcggcccga tcggcctgcg gaccgcatcg accgaggttc ccgagcgttt
     2461 ctcccggctg gtcgcgatgg acaccggcct cttcac
//
LOCUS       DAOQMF010000229         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25854_length_2495_cov_2.895902, whole genome shotgun sequence.
ACCESSION   DAOQMF010000229 DAOQMF010000000
VERSION     DAOQMF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25854_length_2495_cov_2.895902"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLV77_04775"
     CDS             complement(<1..759)
                     /locus_tag="PLV77_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNC15190.1"
                     /translation="MSDAPELKPNSYRDTWCGQVLGDKVGGQVRLSGWVHRRRDHGGL
                     IFIDLRDRTGLVQLVFNPDEAGGAHELGHTLRSEYVISVAGEVVTRSDETVNPELPTG
                     EFEVRVKEVQVLAEAETPPFEIEGFHGEVGEEARLKHRYLDLRRDQMQQAIKLRHRLT
                     AAMRSFLDAEGFLDIETPILTLSTPEGARDFLVPSRLQRGSFYALPQSPQLFKQLLMM
                     SGFERYFQIARCFRDEDLRADRQPDFTQLDIEMSF"
     gene            complement(775..981)
                     /locus_tag="PLV77_04780"
     CDS             complement(775..981)
                     /locus_tag="PLV77_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15191.1"
                     /translation="MKRTIKPGKTAAVKVTVKPAFRELVDAMTYVDARKLSVAHTVKS
                     KKVGKRTAKKPARPSLSLKVRIKN"
     gene            968..1276
                     /locus_tag="PLV77_04785"
     CDS             968..1276
                     /locus_tag="PLV77_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC15192.1"
                     /translation="MVRFTALLITAPAFDFFAWTATAPRSTFPELTDREREILALVAE
                     GRSNEQIANRLTLSLKTIRNHVSNIFNKLQVPDRAAAIAKAREAGLGVQPLEASDPPT
                     "
     gene            complement(1269..>2495)
                     /locus_tag="PLV77_04790"
     CDS             complement(1269..>2495)
                     /locus_tag="PLV77_04790"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNC15193.1"
                     /translation="SIPDGTYKITRAGTGEDGNATGDFDITGSNALDIQPDASNAHVV
                     VDGNGIDRVFQDLSSGLVKFLAIQITGGKLTGFNDGAGIQNSVGVTSLENVTVNDNSS
                     EAQGGGVAVYNNVQVINSTIAQNNADGNGGGFYVPGGASLVARSTTIFGNQADADGDG
                     NGYGGGFAETGGLNVSFTNVLNAGNSGTAILPTDDAYDCYSGPNFYPRYTLSGQPMGP
                     LDCLVGFNPGTNMVSSDVKLDPFLRDNGGQTPTLALLPGSPAIGAGGTASPDECPGFD
                     QNDRPRPSGKCDIGAVQFVAEPGLIITKLLPKKKVIKRKKTRAITVQVRNDGTGPAKQ
                     VKICLKLNRAAKKGLKVKGKACKSVGTVAVGARKSAKIKLMAKPKARKKAYSLKATFK
                     GSNAPLATRAFKVRVR"
BASE COUNT          405 a          788 c          801 g          501 t
ORIGIN      
        1 gaagctcatt tcaatgtcga gctgggtgaa gtcgggctgg cggtcggccc gcaggtcctc
       61 gtcgcggaaa cagcgggcga tctggaagta ccgctcgaag ccggacatca tcagaagctg
      121 cttgaagagc tgcggtgact gaggcagggc gtaaaacgag ccgcgctgga ggcgcgatgg
      181 cacgaggaag tcgcgggcac cttcgggagt ggagagggtg aggatcgggg tttcgatgtc
      241 gaggaatccc tcggcgtcga ggaaggagcg catcgcggcg gtgagccggt ggcgcagctt
      301 gatcgcctgc tgcatctggt cgcggcgcaa gtccaggtag cgatgtttca gccgggcctc
      361 ttcaccgacc tcgccgtgaa agccttcgat ctcgaagggc ggggtctcgg cctcggccag
      421 cacttgaacc tctttgaccc gcacctcgaa ctccccggtc ggcagttcag ggttgaccgt
      481 ctcatccgag cgggtcacca cttcaccggc gacgctgatc acgtattcgg aacgcagggt
      541 gtgaccgagc tcgtgcgctc ccccggcctc atccgggttg aagacgagct ggaccaggcc
      601 ggtccggtcg cgcaggtcga tgaagatcag gccaccgtgg tcacgacggc ggtggaccca
      661 gccggaaagg cgcacctggc ctccgacctt gtcccccagg acctggccac accaggtatc
      721 tcgatacgaa ttcggtttca gctccggcgc gtcgctcatc gaagcgccag tctttcagtt
      781 cttgatccgt accttcagtg agaggctcgg tctggccggc ttctttgccg tgcgtttgcc
      841 caccttcttc gacttgacgg tgtgggcgac cgaaagtttg cgggcgtcca cataggtcat
      901 cgcgtcgacc agctcccgga acgcgggctt gaccgtcacc ttcaccgcgg ccgtcttgcc
      961 tggtttgatg gtccgcttca ccgcattgct gattaccgcg ccggccttcg acttcttcgc
     1021 ctggaccgcg acggccccgc gatcgacttt tcccgagctg accgatcgcg agcgggagat
     1081 ccttgcgctc gtggccgaag gacggtcaaa cgagcaaatc gcgaatcggc tcaccctcag
     1141 cctgaagacg atccggaatc acgtcagcaa catcttcaac aagctgcagg tgcccgaccg
     1201 ggccgccgcg atcgcgaaag cccgcgaggc ggggctcggg gttcagccgc tcgaagcttc
     1261 tgaccctcct acctgacccg caccttgaat gcccgcgtgg cgagcggggc gttagagccc
     1321 ttgaaggtgg ccttgagcga gtaagccttc tttctcgcct tcggcttggc cataagcttg
     1381 atcttcgccg acttcctcgc gccaaccgca acggtgccga ccgacttgca agccttgccc
     1441 ttgaccttca gccccttctt cgcggcccgg ttcagcttca ggcagatctt cacctgcttg
     1501 gccggtccgg ttccgtcatt gcggacctga accgtgatcg ccctggtctt cttccgcttg
     1561 atcaccttct tcttcggcag cagcttcgtg atgatcagac cgggctcggc cacgaactgg
     1621 accgcgccga tgtcgcactt gccggacgga cggggccggt cgttctgatc aaagcccggg
     1681 cattcatcgg gactggcggt acctccggca ccaatcgcgg gcgatcccgg cagcagggcc
     1741 agggtcgggg tctgaccgcc gttgtcccga aggaaggggt ccagcttgac gtctgagctg
     1801 accatgttgg tgcccggatt gaatccaacc aggcagtcaa gcgggcccat cggctgcccg
     1861 gaaagcgtgt agcgcgggta gaagttcggg ccgctgtagc agtcgtaggc gtcgtcggtc
     1921 gggaggatgg cagtgccgga gttgcccgca ttcagaacat tggtgaagct gacgttgagc
     1981 ccgccggttt cggcgaagcc tccgccgtag ccgttgccgt caccgtcagc gtcggcctgg
     2041 ttgccgaaga tcgtggtgct ccgggccacc agcgaggcgc cgcccggaac gtagaagccg
     2101 ccgccgttgc cgtcggcatt gttctgggcg atcgtgctgt tgatgacctg aacgttgttg
     2161 tagacggcga cgccgccgcc ctgggcttca gacgagttgt cgttcacggt cacgttctcg
     2221 agcgaggtca ctcccaccga gttctggatg cctgcaccgt cgttgaaacc ggtcagcttg
     2281 ccacctgtga tttggatcgc gaggaacttg accaggccgc tggagaggtc ctggaagacg
     2341 cggtcgatcc cgttgccgtc aaccaccacg tgcgcattcg atgcgtcagg ttgaatgtcg
     2401 agggcattgc ttccggtgat gtcgaagtct ccggtcgcgt tgccatcctc gcccgttccc
     2461 gcccgggtga tcttgtaggt gccatcgggg atgct
//
LOCUS       DAOQMF010000230         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25900_length_2492_cov_3.710710, whole genome shotgun sequence.
ACCESSION   DAOQMF010000230 DAOQMF010000000
VERSION     DAOQMF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25900_length_2492_cov_3.710710"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PLV77_04795"
     CDS             complement(<1..682)
                     /locus_tag="PLV77_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNC15194.1"
                     /translation="MREVRIRDTMSGELLTLGTDEEIGIYACGPTVYSRIHIGNARPF
                     VIFSLLARFLRSEGYGAKLAINVTDVNDKIYTAAAGRGVSSTELAAEMTAAYFEDTGR
                     LGIGRPDSEPLATETIGPIIDLIATLVEEGHAYESGGDVYFAVRSFDGYGKLSHRPTE
                     EMEQGEGDDAGSLKRDPLDFALWKAKKDGEDLSWESPWGPGRPGWHIECSAMAEAELG
                     QPFTIHGGG"
     gene            complement(700..1860)
                     /locus_tag="PLV77_04800"
     CDS             complement(700..1860)
                     /locus_tag="PLV77_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15195.1"
                     /translation="MSDTTSAPSAEEAGSGNGLPPELRQVGEASAKLNSRIETLAKEL
                     SASESKAREAEIASREAELKALATARDSLTEARKAKAEAERDRAAAATADQRVEAVKA
                     QATRDVAEAREEIKTKARETLRKLSAEAREKIEASRKARLEAEEKAKELEQKDADQRE
                     AQARVEADLKRVEGTLKQERETRDEAMAKVAAEGEERLAARTRHLEEVAERLAKEARQ
                     AAEREAKNRIEAADERTRKAQERAAQAEAHANEFESLARQRIAEIEADVDRRVMEGTE
                     QVRREAAERVEELIERFGREAEENARRRADERLREEDERIQREADRRAELARQSAEDD
                     VQAAADRARQEALRAASSTAPEWERGDSGEGDGGPAPRRRRGGPGAGGYRTF"
     gene            complement(1906..>2492)
                     /locus_tag="PLV77_04805"
     CDS             complement(1906..>2492)
                     /locus_tag="PLV77_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC15196.1"
                     /translation="NFYGSTEVAMGSIATPEDLRHDPATAGRPPYGTSVRLVDEFGDE
                     VPHGETGRIFIRNEMTFDGYTGGGNKENLDGYLSSGDVGHFDEDGRLYIDGRDDEMII
                     SGGENVYPAEVEDLISGHDQVVESAVIGVDDEKFGQALRAYVVLKTPDSLDEDGVRKY
                     VKANLASYKAPRDVIFLPELPRNATGKILKKDLA"
BASE COUNT          305 a          865 c          819 g          503 t
ORIGIN      
        1 acccgcctcc gtgaatcgtg aacggctggc ccagttcggc ctcggccatc gccgagcact
       61 cgatgtgcca gcccggccgg cccgggcccc aaggcgattc ccagctcagg tcctcgccgt
      121 cttttttggc cttccagagg gcgaaatcga gcgggtcgcg cttgagcgaa ccggcgtcat
      181 cgccttctcc ctgttccatt tcctcggtcg gacggtggga gagcttgccg tagccatcga
      241 agctccgaac cgcgaagtag acgtcaccgc cggactcgta ggcgtggcct tcctcgacca
      301 gggtcgcgat caggtcgatg atcggcccga tcgtctcggt cgccaggggc tccgagtcgg
      361 ggcggccgat gccgagcctt ccggtgtcct cgaaataggc ggcggtcatc tcggccgcca
      421 gttcggtcga gctgaccccc cgcccggccg cggcggtgta gatcttgtcg tttacgtcgg
      481 tgacgttgat cgccagcttc gccccgtagc cctcgctccg gagaaaacgg gcgagcagcg
      541 agaagatcac gaaaggacgg gcgttgccga tgtggatccg gctgtaaacg gtcgggccgc
      601 aggcgtagat gccgatctct tcatcggtgc cgagagtgag gagttcccca ctcatcgtgt
      661 ccctgatcct gacctcgcgc attgccgcgg ggagactact cagaaggtgc ggtagccgcc
      721 ggccccgggc ccgccgcgcc ggcgccttgg tgccggtccg ccgtcgcctt cgccggaatc
      781 gccacgctcc cattccggag cggtgctgga ggcggcgcgg agagcttcct gccgggcacg
      841 gtcggctgcg gcctggacgt catcctcggc cgactggcgg gccagctcgg cccggcgatc
      901 cgcctcgcgc tggatccgct cgtcttcttc gcgcaggcgt tcatcggcgc gtcgccgtgc
      961 gttctcctcg gcttcgcggc cgaaacgctc gatcagctcc tcgacccgct cggccgcctc
     1021 gcggcgaacc tgctcggtgc cttccatcac gcgacggtca acgtcggcct cgatctcggc
     1081 gatccgctgg cgggccagcg actcgaattc attcgcatgc gcttcggcct gggcggccct
     1141 ctcctgggcc ttgcgggtcc gctcgtcggc ggcctcgatc cggttcttgg cttcgcgctc
     1201 ggcggcctgg cgggcttcct tggccagccg ctcggccacc tcctcaaggt gccgggtgcg
     1261 ggccgcgagg cgttcctcgc cctcggcagc aacctttgcc atcgcctcgt cgcgggtttc
     1321 gcgctcctgc ttgagcgtgc cttcgacgcg cttcaggtcg gcttcgaccc tggcctgggc
     1381 ttcgcgctgg tcggcatcct tctgctcaag ctccttggcc ttctcctccg cctcgagccg
     1441 tgccttgcgc gaggcttcga tcttctcgcg ggcctcggcg ctcagctttc gcagggtttc
     1501 gcgggccttg gtcttgatct cttcccgggc ctcggcgacg tcacgggtgg cctgggcctt
     1561 gaccgcttcg acccgttggt cggcggtcgc ggcggccgcc cggtcccgtt cggcttccgc
     1621 cttggccttg cgggcttcgg tcagggagtc gcgggcggtg gcgagagcct tgagttcggc
     1681 ttcacgggaa gcgatctcgg cctcgcgtgc ctttgactcg ctggcggaga gttccttggc
     1741 cagcgtttca atccgcgagt tgagtttggc cgacgcctcg ccgacctggc gcagctcggg
     1801 gggcagcccg tttccggagc ctgcctcttc cgcagacgga gctgaagtgg tatcgctcat
     1861 cgcctaaaaa ctactacgca accccacgga tcgcccgatc cgcggtcagg caagatcctt
     1921 tttcaggatc ttgcccgtgg cgtttctggg aagttccggc aggaagatca cgtcgcgggg
     1981 ggccttgtag gaggcgaggt tggccttcac gtacttgcgg acgccgtctt cgtcgagcga
     2041 atcgggcgtt ttcagcacca cgtacgcccg gagcgcctgg ccgaacttct cgtcatcgac
     2101 tccgatcacg gccgactcga ccacctggtc gtgaccggag atcaggtctt cgacctcggc
     2161 ggggtacacg ttctccccgc cggagatgat catctcgtcg tcgcggccgt cgatgtagag
     2221 ccggccgtct tcgtcgaagt ggccgacgtc gccgcttgag aggtagccgt caaggttctc
     2281 cttgttgccc cctccggtgt agccgtcgaa ggtcatttca ttgcggatga agatccgccc
     2341 ggtctccccg tgagggacct cgtcgccgaa ctcgtcgacc agccggacgc tggtcccgta
     2401 cggcggccgg cctgccgtgg ccgggtcatg gcgcagatcc tcgggggtgg cgatcgaacc
     2461 catcgcgacc tcggtcgaac cgtagaagtt gt
//
LOCUS       DAOQMF010000231         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_25922_length_2491_cov_3.062397, whole genome shotgun sequence.
ACCESSION   DAOQMF010000231 DAOQMF010000000
VERSION     DAOQMF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25922_length_2491_cov_3.062397"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PLV77_04810"
     CDS             <1..958
                     /locus_tag="PLV77_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC15197.1"
                     /translation="IQPDFRRNEALAFIRGGLNDVSLSRSQISWGVPLSWDPDQVMYV
                     WFDALLNYYTALGYAREGEDLTGRFWPASWHIIAKDILKFHTVYWPAFLMAAGIEVPQ
                     RIFIHGFLLMGDKKMSKSLGNVLDPFEVIEKFGADALRFYCFREVSFGQDGSISTEGF
                     ETRYESELANDFGNLASRTIAMISRYRDGVVPEAGPDPLLLADFEGITDRVSALLDDA
                     QIGQALEEIWVLVRRLNRYVEEAQPWVLAKDEADAERLDQVLYSLAEGLRVLTLLLHP
                     YLPASTDILLEALAEPDQGLAPFGSRPGGVKVEKIDPLFPKL"
     gene            976..1404
                     /locus_tag="PLV77_04815"
     CDS             976..1404
                     /locus_tag="PLV77_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15198.1"
                     /translation="MRKARSLTAGVAIAAIGLGLIGASVAPASAAARPLSVRFFTPDG
                     KQRLEVKRKVGIGVGCTKNCAVVVDMKLVMPNTKPIKARVKGGIKAFSAKSPYIVLNN
                     PALRYLKLTYPRSRFSIKVTATDISNGRKTVKRRVYGFRA"
     gene            1411..2175
                     /locus_tag="PLV77_04820"
     CDS             1411..2175
                     /locus_tag="PLV77_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC15199.1"
                     /translation="MIDSHSHVSRCPESPEKVIGAAREAGVERILTVGLDEASNREQI
                     ALCEQFDGLFAAVGRHPNDADGFDDTAAADIEQLAAHPKVVAIGETGLDFFRDTAEPD
                     SQRLAFIKQADIAARLDLPLVIHCRDQQGRDDAVSEIFATLEEFPDLTVILHCFSAPD
                     WVERAAERDWYCSFAGNVTYPANEDLRTAAARVPADRIMVETDAPYLTPQSRRRDRNQ
                     PAFVVETAELVAGLRGIDYAELDELVTANAARVYGW"
     gene            2172..>2491
                     /locus_tag="PLV77_04825"
     CDS             2172..>2491
                     /locus_tag="PLV77_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNC15200.1"
                     /translation="MKLGQNFLADPNLLDAIVRDAGLSPEDVVLEVGAGEGVLTERLA
                     EVASQVHVIELDRGLEPGLSGVIGRPNVNLVWADAVKYDLAGLDPAPTRMVANLPYAV
                     ATPV"
BASE COUNT          435 a          846 c          797 g          413 t
ORIGIN      
        1 gatccagccc gacttccgcc gcaacgaggc gctcgccttc atccgcggcg gtctcaacga
       61 tgtctctctt tcccgctccc agatctcctg gggggtgccg ctgagctggg atccggacca
      121 ggtcatgtac gtctggttcg acgccctgct caactactac acggccctcg gctacgcccg
      181 cgagggagaa gacctgaccg gccgcttctg gcccgcctcc tggcacatca tcgccaagga
      241 catcctcaag ttccacaccg tctactggcc ggccttcctg atggctgccg gtatcgaggt
      301 gccgcagcgg atcttcatcc acggcttcct gctgatgggt gacaagaaga tgtcgaagtc
      361 gctcggcaac gtgctcgatc cgttcgaggt gatcgagaag ttcggggcag atgccctgcg
      421 cttctattgc ttccgcgagg tcagcttcgg ccaggacggt tcgatctcga ccgagggttt
      481 cgaaacccgc tacgagtccg aactggccaa cgacttcggc aacctcgcct cacgcaccat
      541 cgcgatgatc agccgctacc gcgacggggt cgtgccggag gccgggccgg acccgctgct
      601 cctggcggat ttcgaaggca tcaccgaccg cgtttccgcc ctgctggacg atgcccagat
      661 cgggcaggcc ctggaagaga tctgggttct ggtgcgtcgc ctgaaccgct acgtcgagga
      721 ggcgcaaccc tgggttctcg ccaaggacga ggccgacgcc gaaaggctcg accaggttct
      781 ctacagcctg gccgaggggc tgcgggtgct caccctgctg ctccatccct acctgccggc
      841 ctccaccgac atcctgctcg aagccctcgc cgagcccgac cagggcctgg ccccgtttgg
      901 cagccggccc gggggcgtga aggtggaaaa gatcgatccc ctgtttccga aactctgagg
      961 aactaggatc ggggcatgag aaaagccagg agtcttacag caggagttgc catcgccgca
     1021 atcggtctgg gattgattgg cgcttcagtc gcaccggcct ctgcagccgc gcgcccgctc
     1081 agcgtcaggt tcttcactcc ggacggcaag cagcgcctcg aggtgaagcg caaggtcgga
     1141 atcggcgtcg gctgcaccaa gaactgcgcg gtggtggtcg acatgaagct ggtcatgccc
     1201 aacaccaagc cgatcaaggc cagggtgaag ggcggaatca aggccttcag cgccaagtcg
     1261 ccctacatcg tgctgaacaa tcccgcgctc aggtacctga aactgaccta cccccggtcc
     1321 cggttctcga tcaaggttac tgcgaccgac atctcgaacg gcaggaagac ggtcaagcgc
     1381 agggtctacg gcttccgcgc atagccgaac ttgatcgatt cgcattcgca tgtctcgcgc
     1441 tgccccgagt caccggagaa ggtgatcggg gcggcccgcg aggccggggt cgagaggatc
     1501 ctcacggtcg gtctcgacga ggcttccaac cgggagcaga tcgccctttg cgagcagttc
     1561 gacgggctgt tcgcggcggt cggaaggcat ccgaacgacg ccgacggctt cgatgacacg
     1621 gcggccgccg acatcgagca gctcgccgcc cacccgaagg tggtggcaat cggcgagacc
     1681 gggctcgact tcttccggga cacggccgag cccgacagcc agcgactcgc tttcatcaag
     1741 caggccgaca tcgccgcccg gctcgacctg ccgctggtca tacattgccg ggatcagcaa
     1801 ggccgggatg acgcggtctc cgaaatcttc gcgacgctcg aggagttccc ggacctgacc
     1861 gtgatcctcc actgtttctc cgccccggac tgggtcgagc gggcggccga acgcgactgg
     1921 tactgctcgt ttgccggcaa cgtcacctac cccgccaacg aggatctgcg caccgccgcg
     1981 gccagggtcc cggccgaccg gatcatggtc gagaccgacg cgccgtacct gacgccgcag
     2041 tcccggcgcc gggaccggaa ccagccggcc ttcgtggtcg agaccgcgga actggtcgcc
     2101 gggctgcggg gaatcgacta cgccgaactg gacgaactgg tcaccgccaa cgcagcccgc
     2161 gtctacggat ggtgaagctc ggccagaact tcctggccga cccgaacctg ctcgacgcga
     2221 tcgtccggga tgccggcctc tcgcccgaag acgtggtcct ggaagtaggt gcgggggagg
     2281 gagtgctgac cgagcgcctg gccgaagtcg cttcgcaggt tcacgtgatc gagctcgacc
     2341 ggggcctcga gccggggctg tccggcgtga tcggcaggcc gaacgtgaac ctggtctggg
     2401 ccgacgcggt gaagtacgac ctggccggac tcgacccggc cccgacccgg atggtcgcca
     2461 acctgcccta cgccgtggcg accccggtga t
//
LOCUS       DAOQMF010000232         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26006_length_2486_cov_3.662279, whole genome shotgun sequence.
ACCESSION   DAOQMF010000232 DAOQMF010000000
VERSION     DAOQMF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26006_length_2486_cov_3.662279"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PLV77_04830"
     CDS             <1..1100
                     /locus_tag="PLV77_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNC15201.1"
                     /translation="GLKFYSPPKKLPTAHGKLIWQRKAGGSVPLKSAASTKLVLYTSR
                     STTGKQIAVSGSVSIPKGKAPKGGWPIISYAHGTTGIADKCAPSRNTKGGVADPYISY
                     MDPLQNAWLRAGYAVARTDYQGLGVPGVNHPFLVGVPAGRSVLDIVSASRQLGLKIGN
                     RYLIAGHSQGGHAALFAAGLASSYSKDIKLRGTVSYAPASHFKEQASFLPGLTSPNGL
                     TALATLLVRGMSISYPQLNANEALSDTILPFYPQTLTKCLSELAGSDNLGGIAPSELI
                     RSGYDMTPLNGILEAQNPSVTTSAPILLAQGTADSTTFPFLTDQLDTELVGKGDTVDY
                     KKYEGVDHGGVVAAAQNDVMSFFESRLPSGK"
     gene            complement(1153..1581)
                     /locus_tag="PLV77_04835"
     CDS             complement(1153..1581)
                     /locus_tag="PLV77_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNC15202.1"
                     /translation="MRCMVIVKATEDSEADVMPSEKDLGDMGSFNEELVNAGVMLDGD
                     GLKSSRHGVRITYSGDEAQVTDGPFSETKELIAGYWVWEVKSLEEAIEWARRIPFHHG
                     ESVEIRPFHELEDFGEAVTDEFRERQARMEATIEEQKRDA"
     gene            complement(1657..>2486)
                     /locus_tag="PLV77_04840"
     CDS             complement(1657..>2486)
                     /locus_tag="PLV77_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HNC15203.1"
                     /translation="IARAFLVSDSAIAQRIVRAKRTLADSGIPFEPPSTDEMGERLAA
                     VLGVIYLIFNEGYSATAGDQWMRTELSVEALRLGRIMVGLAPREPEVHGLVSLMEIQA
                     SRFPARTGPQGEPVLLMDQDRSRWDRLLIRRGLKELETARSLGGNGPYVIQASIAACH
                     ARALRPEQTDWQEILGYYDELAALGDSPVAELNRAVAMAMSGDPRSALDAVDRVATDS
                     RMKGYYLLPSVRGDLLERLGRSGEAAAEFERAASLTDNQAERRVLIERAERAKDFPG"
BASE COUNT          434 a          876 c          732 g          444 t
ORIGIN      
        1 ccgggctgaa gttctactcg ccgccgaaga agctccctac cgcccacgga aaattgatct
       61 ggcagcgcaa ggccggtggg tccgtgcctc tgaagagcgc cgcttcgacc aaactggttc
      121 tctacacctc caggtcgacc accggcaagc agatcgccgt gtcgggttcg gtgagcatcc
      181 cgaagggcaa ggccccgaag ggcggctggc cgatcatcag ttacgcccac ggcaccaccg
      241 gcatcgccga caagtgcgcc ccctcgcgta acaccaaggg tggcgtcgcc gacccttaca
      301 tctcgtacat ggatccgctg cagaatgcct ggctgagggc cggctacgcg gttgcccgca
      361 ccgattatca gggccttggc gtgcccggtg tgaaccaccc gttcctggtc ggcgttccgg
      421 ccggccgcag cgtgcttgac atcgtcagcg cctcgcgtca gctcggtctg aagatcggca
      481 acaggtacct gatcgccggc cattcgcagg gcgggcacgc ggcactgttc gcggccggtc
      541 ttgcctcgag ctactcgaag gacatcaagc tgaggggcac cgtttcctac gcaccggctt
      601 cgcacttcaa ggagcaggcc tcattcctcc cgggactcac ctcccccaac ggactgaccg
      661 ccctggccac gctgctggtt cgcggcatga gcatctccta cccgcagctg aacgcgaatg
      721 aagcgctttc cgacacgatt ctgcccttct acccgcagac gctgaccaag tgcctgtcgg
      781 agctcgcagg gtctgacaac ctcgggggca tcgcgccttc cgagctgatc cgctcgggct
      841 atgacatgac cccgctcaac gggatcctcg aagcgcagaa cccgtcggtt acgacttcgg
      901 ctccgatcct gctggcgcag ggcacggccg attcgaccac cttcccgttc ctcaccgacc
      961 agctcgacac cgagctggtc ggcaagggcg acacggtcga ctacaagaag tacgaaggcg
     1021 tcgaccacgg cggagtcgtg gcagccgccc agaacgacgt catgtcgttc ttcgaatcgc
     1081 gactgccgtc cggcaagtag gtccggccgg cagaagttct gaaagagggg gcctccgggc
     1141 cccctctttt cgtcaggcgt cccgcttctg ctcttcgatc gttgcttcca tccgggcctg
     1201 ccgttcccgg aactcgtcgg ttaccgcctc gccgaagtct tcgagttcgt ggaagggccg
     1261 gatctcgacc gactcgccgt gatggaaggg gatccggcgt gcccactcga tcgcttcctc
     1321 gagcgacttc acctcccaga cccagtatcc ggcgatcagc tccttggtct cggagaaggg
     1381 accgtcggtc acctgggctt catctccgga gtaggtgatc cggaccccgt gacgggatga
     1441 cttcaatccg tcgccgtcca gcatgacccc ggcgttgacc agctcctcgt tgaaactccc
     1501 catgtcgccc aggtccttct cggatggcat cacatctgct tcggaatcct cggttgcctt
     1561 gacgatcacc atgcaacgca tctcaatctc cttcggtaga cggccggcat ttccggcctt
     1621 tgcatcgaag acgaacggga aggctggaat tcgacatcag cccggaaaat cttttgcccg
     1681 ttcggcccgc tcgatcagta cccgccgttc cgcctgatta tcggtcagtg aagcggcgcg
     1741 ttcgaactcg gccgccgcct ccccgctccg gccaagccgc tccagcagat caccgcgcac
     1801 gctcggaaga aggtaatagc ccttcatgcg actgtccgtc gccacccgat ccaccgcgtc
     1861 gagggccgac cgggggtctc cggacatcgc catcgcaact gcccggttga gctcggcgac
     1921 cggtgaatcc cccagcgccg ccagctcgtc gtagtagcca aggatctcct gccagtcggt
     1981 ctgctccggc ctcaacgcac gggcatggca ggcagcgatg gacgcctgga tcacgtacgg
     2041 cccgttcccg ccaaggctcc tcgcagtctc cagctctttc agcccccttc tgatcaggag
     2101 ccggtcccag cgtgagcggt cctggtccat cagcaacacc ggttcccctt gcgggccggt
     2161 tcgcgccggg aagcgcgaag cctgaatctc catcagggac acaaggccat gcacctccgg
     2221 ctcccgggga gccagcccga ccatgatccg gcccagccgg agcgcctcaa cggaaagctc
     2281 ggtgcgcatc cattgatcgc cggcggttgc cgaatagccc tcgttgaaga tcaggtagat
     2341 cacaccaagg acggccgcca gccgctcgcc catctcgtcg gtggacggcg gctcaaaggg
     2401 gatcccggaa tcggcgaggg tgcgcttggc ccggacgatg cgctgggcga tcgccgagtc
     2461 cgagacaagg aaagcccggg cgatct
//
LOCUS       DAOQMF010000233         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26065_length_2483_cov_3.756178, whole genome shotgun sequence.
ACCESSION   DAOQMF010000233 DAOQMF010000000
VERSION     DAOQMF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26065_length_2483_cov_3.756178"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLV77_04845"
     CDS             complement(<1..782)
                     /locus_tag="PLV77_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNC15204.1"
                     /translation="MTATWAWLVLLLPLLGSMTIGLTFRVLPARTAGLIGVTAIVAAF
                     VCGILALIGLQGEPSESRHVASSLWEYASVGGLSIDLGIYVDPLSIFMVLVVTGVSSL
                     IHIYSYSYMKSDQGYHRFFSYLNFFVFSMLVLVLAGNFIMLIVGWALVGFASYALISY
                     WYRRNTATKAGMKAFVINVIGDIGLYLAAILIFKNIGSVDYGTVFEKAPEVFTTNEWE
                     IVAICLCLLVGAFAKSAQMPFHTWLADAMEGPTPVSSLIHAAT"
     gene            complement(779..1078)
                     /gene="nuoK"
                     /locus_tag="PLV77_04850"
     CDS             complement(779..1078)
                     /gene="nuoK"
                     /locus_tag="PLV77_04850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNC15205.1"
                     /translation="MDIEWYLILSALLFTVGFIGVLVRRNPVVILLCLELMLSAGALA
                     LLAFSRMLGNQDGQVFVLIVLVVAACEVVVGLGLVVAMYRRRLPLDVDEMKELKG"
     gene            complement(1078..1692)
                     /locus_tag="PLV77_04855"
     CDS             complement(1078..1692)
                     /locus_tag="PLV77_04855"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNC15206.1"
                     /translation="MEEIAFFIGAIGALGAAIAVIAVRNPFYSVLALVIHLVFLAGIF
                     LLLMAQFLAAALIVVYAGAVMVLYVFVAAYVGGIEEPKWDSSPELRIVGPILAVALFI
                     AVSIAILGSGLSALGTPGPDDVPSGFGTPEAVGHLMMEDFLIAFEAASLLLLVAAVAA
                     IVLAGRRREDRPSDLSKRKSMVARDENVNAAIAGDPAAGRSEEA"
     gene            complement(1692..2222)
                     /gene="nuoI"
                     /locus_tag="PLV77_04860"
     CDS             complement(1692..2222)
                     /gene="nuoI"
                     /locus_tag="PLV77_04860"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNC15207.1"
                     /translation="MSIRPDEIKVVPRGPDPGGVGGAYRAFGETLRGLRTTMGRLFDK
                     PVTIQYPEEKIPVYPRFRGRHKLHKFEDSGLEKCVGCSLCAAACPAECIRVVAAENDP
                     ENRVSGGERYASVYEINLARCIFCGYCEMACPFDAITMGHDYEMADYNRSDLIFTKEM
                     LLAEPIERTPLRGEGE"
     gene            complement(2219..>2483)
                     /locus_tag="PLV77_04865"
     CDS             complement(2219..>2483)
                     /locus_tag="PLV77_04865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNC15208.1"
                     /translation="FAEYMEILIVSGIASTMFLGGWMGPGPGWMDPIWMLLKMWMLAM
                     FFIWIRATLPRFRYDQLMSFGWKILLPLATLNVLVTAILVVAT"
BASE COUNT          515 a          777 c          830 g          361 t
ORIGIN      
        1 gtcgccgcgt ggatcaggga ggaaaccggg gtcgggcctt ccatggcgtc ggcgagccat
       61 gtgtggaacg gcatctgggc ggacttggcg aaggcgccga ccagcaggca gaggcagatc
      121 gcgacgatct cccactcgtt ggtggtgaac acttccggcg ccttctcgaa gaccgtgccg
      181 taatcgaccg aaccgatgtt cttgaagatc aggatcgccg cgaggtaaag gccgatgtcg
      241 ccgatcacgt tgatcacgaa ggccttcatg ccggccttgg tcgccgtgtt ccggcggtac
      301 cagtagctga tcagcgcgta ggaagcgaag ccgaccaggg cccagccgac gatcagcatg
      361 atgaagttgc cggccaggac caggaccagc atcgaaaaga cgaagaagtt caggtagctg
      421 aagaagcggt ggtagccctg atccgacttc atgtagctgt acgagtagat gtgaatcagg
      481 ctggaaacgc cggtgacgac cagcaccatg aagatcgaaa gcgggtcgac atagatcccg
      541 aggtcgatcg aaagtccgcc cacgctggcg tactcccaga ggctcgaggc gacgtgccgg
      601 gactccgaag gttcaccctg cagaccgatc agggcgagga tgccgcagac gaaggcggcg
      661 acgatcgcgg tgacgccgat cagcccggcg gtgcgggccg gcagcaccct gaaggtcagt
      721 ccgatggtca tcgaaccgag caggggcagc agcaggacga gccaggccca ggtggcggtc
      781 atcccttgag ctccttcatc tcgtcgacgt cgagcggcag ccgtcggcga tacatcgcga
      841 cgaccagccc gagcccgacc acgacctcac aggccgccac cacgagcacg atcagcacga
      901 agacctggcc atcctggttg cccagcatgc ggctgaaagc gagcagggcc agggccccgg
      961 cgctcagcat gagctcgagg cagagcagga tcaccaccgg gttgcggcgc accagcaccc
     1021 cgatgaaccc gaccgtgaag agcagggccg aaaggatcag gtaccactcg atgtccatca
     1081 ggcctcctcg ctcctgccgg cggccggatc cccggcgatc gcggcgttca cgttctcgtc
     1141 ccgggcgacc atggacttgc gcttcgaaag atcggaagga cggtcctcac ggcggcggcc
     1201 ggccagcacg attgccgcga ccgcagcgac cagcagaagc agcgaggccg cttcgaaggc
     1261 gatcaggaag tcctccatca tcaggtggcc gaccgcttcc ggcgtgccga agccggaagg
     1321 cacgtcatcc gggccggggg tgccgagcgc ggaaagcccg gagccgagaa tggcgatcga
     1381 aaccgcgatg aaaagggcca ccgcaaggat cggcccgacg atcctgagct ccggcgagga
     1441 gtcccatttg ggctcctcta tgccgccgac gtaggcggcg acgaagacgt agagcaccat
     1501 caccgcaccg gcgtagacga cgatcagggc ggcggccagg aactgggcca tcaagagcag
     1561 gaagatcccg gccaggaaga ccaggtggat gaccagggcc agcacgctgt agaacgggtt
     1621 ccggaccgcg atcaccgcga ttgccgcacc cagggcgccg atcgcgccga tgaagaaggc
     1681 gatctcttcc actactcgcc ctctccccgc agcggagtcc gttcgatcgg ctcggccagc
     1741 agcatctcct tggtgaaaat caggtccgag cggttgtagt cggccatctc gtagtcgtgg
     1801 cccatggtga tcgcgtcgaa ggggcaggcc atctcgcaat agccgcagaa gatgcagcgg
     1861 gcgaggttga tctcgtagac cgaggcgtag cgctcgccac cggagacccg gttctcggga
     1921 tcgttctcgg ccgcgaccac gcgaatgcat tcggccgggc aggcggcggc gcaaagcgag
     1981 cagccgacgc atttctcgag ccccgaatcc tcgaacttgt gaagcttgtg gcgtccgcgg
     2041 aagcgcgggt agaccgggat cttctcctcg gggtactgga tcgtcaccgg cttgtcgaag
     2101 agccggccca tggtggtcct gagcccgcgc agggtctcgc cgaaggcgcg gtaggcacct
     2161 cccactccgc cgggatcggg gccgcgcgga actaccttga tctcatcggg gcgaatgctc
     2221 atgtggccac caccaggatt gccgtcacga ggacgttgag ggttgcgagg ggaagcagga
     2281 tcttccagcc gaaactcatc agctggtcgt agcggaacct gggcagggtg gcgcggatcc
     2341 agatgaagaa catggccagc atccacatct tgagcagcat ccagatcggg tccatccagc
     2401 cggggccggg acccatccag ccgccgagga acatggtcga agcgattccc gagacgatca
     2461 ggatctccat gtactcggcg aag
//
LOCUS       DAOQMF010000234         2483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26066_length_2483_cov_3.311367, whole genome shotgun sequence.
ACCESSION   DAOQMF010000234 DAOQMF010000000
VERSION     DAOQMF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26066_length_2483_cov_3.311367"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1287
                     /locus_tag="PLV77_04870"
     CDS             13..1287
                     /locus_tag="PLV77_04870"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNC15209.1"
                     /translation="MEEALEEIRAGRMIIVTDDEDRENEGDLVMAAQFATPEAINFMA
                     KEGRGLICLSLTAQRCDQLGLRLMTAKNEAPLETAFTVSIEAAEGVTTGISAHDRAHT
                     VQVAIDPDATPRDIVVPGHVFPLKAKDGGVLERTGHTEAAVDLARLAGVSPAGVICEV
                     MNEDGTMARVDDLVGYAEKHGLKMVTIADLIAYRRENEKLVERVVSTALPTSFGDFRA
                     VGFRSLVDDKHHVAMVKGEIDTGEPALVRVHSECLTGDVFHSMRCDCGEQLAAAMAMI
                     EDEGRGVLLYLSQEGRGIGLLNKLRAYKLQEEGLDTVDANLKLGLPADLRDYGIGAQI
                     LRDLGLTKIKILTNNPKKIIGLEGHGLEVTEQIKIEAPPNTHNESYLRTKRDKMGHIL
                     HHQGLALDEEMILDEQIHDSEKATEAEGGRGA"
     gene            1280..1735
                     /gene="ribH"
                     /locus_tag="PLV77_04875"
     CDS             1280..1735
                     /gene="ribH"
                     /locus_tag="PLV77_04875"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNC15210.1"
                     /translation="MPEPEFTGRFCVVVGNFYTDLADRLVGGAKEEFARDGISDAEID
                     VYEVPGAFELPLAAGYAARSGRYDGVACLGAVIRGETDHYDFVCNEAARGIQNVQLET
                     GVPCGFGVLTVDNMEQALARAGGGGKRDSGANAARAVLRMNEIRRELSA"
     gene            1808..1999
                     /gene="rpmB"
                     /locus_tag="PLV77_04880"
     CDS             1808..1999
                     /gene="rpmB"
                     /locus_tag="PLV77_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNC15211.1"
                     /translation="MARVCHNCGKGPGFGNSRSHSMVATRRRFDPNLQKVRILEGGAK
                     KRVLVCTRCLKANKVTKSA"
     gene            2238..>2483
                     /locus_tag="PLV77_04885"
     CDS             2238..>2483
                     /locus_tag="PLV77_04885"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNC15212.1"
                     /translation="MSDPSIERFKRVVAAAYGSLEARRQEVNDLNVFPVADGDTGDNM
                     AMTMKAVMEELDSFENGQSLDEIGRTELVSALARAALM"
BASE COUNT          479 a          813 c          799 g          392 t
ORIGIN      
        1 ccgttcgcca cgatcgaaga ggccctggag gagatcaggg ccggccggat gatcatcgtc
       61 accgatgacg aggaccgcga aaacgagggc gacctcgtga tggccgccca gttcgcgact
      121 cccgaagcga tcaacttcat ggccaaggaa gggcgggggc tgatctgcct ttcactcacc
      181 gcccagcgct gcgaccagct cggcctgcgc ctgatgaccg ccaagaacga ggcgccgctg
      241 gaaaccgcct tcaccgtttc gatcgaagcg gccgaaggcg tgacaaccgg catctccgcc
      301 cacgaccggg cccacacggt ccaggtcgcg atcgaccccg acgcgactcc gcgggacatc
      361 gtcgtccccg gacatgtatt cccgctcaaa gccaaggacg gtggcgtgct ggagcgaacc
      421 ggccacaccg aggccgcggt cgatctcgcc cgcctggccg gtgtttcccc ggccggcgtg
      481 atctgcgagg tgatgaacga ggacggcacc atggcccggg tcgacgacct ggtcggctac
      541 gccgaaaagc acggcctcaa gatggtcacg atcgccgacc tgatcgccta ccggcgcgag
      601 aacgagaaac tggtggaacg ggtcgtctcg accgcgctgc cgaccagttt cggtgacttc
      661 cgcgcagtcg gtttccgctc cctggtcgac gacaagcatc acgtcgcaat ggtcaagggt
      721 gagatcgaca ccggcgagcc cgccctggtc cgggtgcatt cggaatgcct gaccggcgac
      781 gtcttccaca gcatgcgctg tgactgcggc gagcagctcg cggcggcgat ggcgatgatc
      841 gaggacgaag gccgcggcgt gctgctctac ctctcgcagg aaggccgcgg gatcggcctt
      901 ttgaacaagc tgagggccta caagctccag gaggaagggc tcgacacggt tgatgccaac
      961 ctcaagctcg gtttgccggc cgacctgcgg gactacggca tcggtgccca gatcctgcgg
     1021 gatctcggcc tgaccaagat caagatcctg accaacaacc cgaagaagat catcggcctc
     1081 gaaggccacg ggctcgaggt caccgagcag atcaagatcg aggccccgcc taacacccac
     1141 aacgagagtt acctgcggac caagcgggac aagatgggtc acatcctcca ccaccagggt
     1201 cttgccctcg acgaggaaat gatcctcgac gagcagatcc acgacagcga gaaggcgacc
     1261 gaagccgaag gcggccgggg tgcctgaacc cgaattcaca ggacggttct gtgtggtggt
     1321 cggcaatttc tacacggacc tcgccgaccg tctggtcggt ggcgcgaagg aagaattcgc
     1381 gagggacggg atctccgacg ccgagatcga cgtctatgag gttcccggcg ctttcgaact
     1441 gccccttgcc gcgggctacg cggcccgttc cggccgctac gacggagtcg cctgcctcgg
     1501 cgcggtgatc cgtggcgaga ccgaccacta cgacttcgtc tgcaacgagg cggcccgcgg
     1561 gatccagaac gtccagctcg aaaccggcgt cccatgcggt ttcggggtgc tcaccgtcga
     1621 caacatggaa caggccctgg cccgggccgg gggcggcgga aagcgcgatt ccggcgccaa
     1681 cgcggcccgc gccgtgctgc gcatgaacga gatccgcagg gagctttcgg cctgaccaag
     1741 ccggattccg gccggcggtg ggccacgagg ccgcccgctc gtcgaatcgg ctaggatgct
     1801 ccgtcccatg gcacgcgtat gtcacaactg cggtaagggt ccgggcttcg gaaattcccg
     1861 gagtcactcg atggtcgcca cccggcgccg cttcgacccg aacctccaga aggtccgcat
     1921 cctcgaggga ggcgccaaga agcgcgtcct ggtttgtact cgttgcctga aggccaacaa
     1981 ggtcacgaag tccgcctgaa tcaccgttgt ggtgacgccg cgggatcggg cgggcatcac
     2041 cgggaagcgg caaacacctc acccgatccc acggctaccc gatctcgtct gttgaccggt
     2101 cctgcagggt tcgggtcggt ggtgcttccc aaaaacctcc ggaatgagga gcgcagcgac
     2161 ggtttttggg aagtactgcc gtcccgcacc cctcgactca ctaccgatag ccgcttcgat
     2221 ctataggctc actcgtcgtg tccgatccga gtattgaacg tttcaagagg gttgttgccg
     2281 ctgcctacgg gtcgcttgaa gcccgcaggc aggaggtcaa cgacctgaat gttttcccgg
     2341 tcgccgacgg cgacaccggg gacaacatgg cgatgaccat gaaggctgtg atggaggagc
     2401 tcgacagctt cgaaaacggc cagtcgctgg acgagatcgg ccgaaccgaa ctggtctcgg
     2461 ccctggcccg cgccgccctg atg
//
LOCUS       DAOQMF010000235         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26175_length_2477_cov_3.638728, whole genome shotgun sequence.
ACCESSION   DAOQMF010000235 DAOQMF010000000
VERSION     DAOQMF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26175_length_2477_cov_3.638728"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PLV77_04890"
     CDS             <1..1079
                     /locus_tag="PLV77_04890"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNC15213.1"
                     /translation="GPDLPEDEEELARVVAENTIFCRIRPEQKRQLVSALAESGAYTA
                     MIGDGVNDVPALKSARMAVAMGSGSQVTKSVADVVLLKDQFSRLPEAIAEGRRIARNI
                     HRLGRLYLTKTVYAAALIILVSVPGFTFPFLPRHLTLAAFLTIGIPSFVLALAPSDGP
                     LYRGRLLRALGAFAIPAGLATALSSILSFFLVDIAFGGSLEDGRTAATTTLIILGLCF
                     VLLLERGPGREHIAIQSYMLAMVAGLGALFAGILAVPQLREFFDMTMLGAEQWFIAML
                     SAALGLVFASLMWRLPQIQAWEVPDEDSFPPFERTPPPDNDRRPMPGPTHHRPRPEES
                     PTEVIPAEADLLDEPPQNGAPTNH"
     gene            complement(1170..1679)
                     /locus_tag="PLV77_04895"
     CDS             complement(1170..1679)
                     /locus_tag="PLV77_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNC15214.1"
                     /translation="MTQVTAKDPGHETGSKSAHEAAFQSMRSAYLLLSERFEKELAEA
                     GLPDLGFYGVLLALSEAPEPSRPRDLLCKVNVTKSGLTRLVDRIEKAGLVERTYCPSD
                     RRGTFLVITEEGRETLARMQPVRERVFTEGFIDLVDEEDAETVARVMESVSQGIYESL
                     NTDGACDEA"
     gene            1797..2354
                     /locus_tag="PLV77_04900"
     CDS             1797..2354
                     /locus_tag="PLV77_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNC15215.1"
                     /translation="MSTTEQAVRIETGTYAVDPVHTQVGFEVKHLGISTFRGSFGGFE
                     GNITVGDEGIVAIEGSIDVSTVAVPEEQLIGHLLSPDFFDAENHPKGTFVSTGVNQVD
                     GETYEVTGNLTLRGVTNPVTLTVEVEGAGIGMDGSSVLSLKANGEVNRNDYGISWGAA
                     LDNGAAVVAEKVRLVLTVEAVKAGE"
BASE COUNT          422 a          851 c          759 g          445 t
ORIGIN      
        1 agggtccgga cctgcccgaa gacgaggagg agctggcccg ggtcgtcgcg gaaaacacga
       61 tcttctgccg gatccgcccc gaacagaagc gccagctggt ttcggcactt gccgagtccg
      121 gcgcctatac ggcgatgatt ggtgacggcg tcaacgacgt gccggccctg aagagcgccc
      181 ggatggccgt cgcgatgggc tccggcagcc aggtgaccaa gtcggtggcc gatgtggtgc
      241 tgctcaagga ccagttcagc cgcctgccgg aagcgatcgc cgagggccgc cggatcgccc
      301 gcaacattca ccgcctcggc cgcctctacc tgaccaagac cgtctacgcg gcggccctga
      361 tcatcctggt ctcggtaccc ggcttcacct ttcccttcct accgcggcat ctcaccctgg
      421 ccgccttcct gacgatcggc atcccctcat tcgtcctcgc cctcgcgccg tcggacgggc
      481 cgctctaccg cggcaggctg ctcagggcgc tcggggcctt cgcgatcccg gccggcctgg
      541 cgaccgcgct ttcctcgatt ctctcgttct tcctcgtcga catcgccttc ggcggcagcc
      601 tcgaggatgg acgcaccgcc gcgacgacca ccctgatcat cctcggtctc tgcttcgtgc
      661 tgcttctgga gcgcggcccg ggccgcgagc acatcgcgat ccagagctac atgctggcca
      721 tggtcgccgg gctcggagcg ctgttcgccg ggatcctggc cgtgccccag ctgcgcgagt
      781 tctttgacat gacgatgcta ggggccgaac agtggttcat cgccatgctt tcggccgcgc
      841 tcggcctggt ctttgccagc ctgatgtggc gcttgcccca gatccaggcc tgggaggttc
      901 cggacgagga ttcgttcccg cccttcgaac gaacccctcc cccggataac gaccgcaggc
      961 caatgcccgg tccgacccac caccggcccc ggcccgagga atctcccacc gaagtgatcc
     1021 ccgccgaagc cgacctgctg gacgaacccc cacaaaacgg agctccgacc aaccattaga
     1081 tatttctagt gggcggagcg ggttccgatt ggaggtccgg cgaagaccgg accgactgca
     1141 gtcattttcg gaccccgccc gactcgcagt caggcctcgt cgcaggcgcc gtcggtgttc
     1201 agtgactcgt agatgccctg ggagacgctt tccatcaccc gggccactgt ctccgcgtct
     1261 tcttcatcga ccaggtcgat gaaaccttcg gtgaagaccc gttcgcggac gggctgcatc
     1321 ctggccagag tctcgcggcc ctcttcggtg atgacgagga aggtgccgcg gcgatccgag
     1381 gggcagtagg tgcgctcgac cagccccgcc ttctcgatcc ggtcgaccag gcgggtcagt
     1441 ccgctcttgg tcacgttgac cttgcagagc aggtcgcggg gccggctcgg ctcgggagct
     1501 tcgctcagcg ccagcagcac tccgtagaac ccgaggtcgg gcagaccggc ttcggccagt
     1561 tccttctcga agcgctccga aagcagcagg taggcgctgc gcattgactg gaatgcggct
     1621 tcgtgagccg atttcgagcc ggtctcgtgc cccgggtctt ttgccgtcac ctgtgtcata
     1681 gagcacattg tagcgcaagt agttgacagc gcaactattt ttgctatggt gtccgtacaa
     1741 cagttgctta cgcaacaatt tctcagtcaa cttgaattga aacgaaggga aacaaaatga
     1801 gcaccaccga acaggcagta cgcatcgaga ccggcaccta cgccgtcgac cccgtccaca
     1861 cccaggtcgg cttcgaggtg aagcacctcg gcatctccac cttccgcggc agcttcggcg
     1921 gcttcgaggg caacatcacc gtcggtgacg agggaatcgt cgcgatcgag ggttcgatcg
     1981 acgtctccac cgtcgccgtt cccgaagagc agctgatcgg ccacctgctc agccccgact
     2041 tcttcgacgc agagaatcac cccaagggca ccttcgtttc gaccggcgta aaccaggtcg
     2101 acggcgagac ctacgaggtc accggcaacc tcaccctgcg cggcgtgacc aacccggtga
     2161 cgctgaccgt cgaggtcgag ggcgccggca tcggcatgga cggcagctcc gtcctcagcc
     2221 tcaaggcgaa cggtgaagtc aaccgcaacg attacgggat ctcctggggc gccgcgctcg
     2281 acaacggtgc cgccgtcgtc gccgagaagg tccggctcgt cctcacggtc gaggcagtca
     2341 aggcgggaga gtaaccatga aggtcctcgc aatcgtcgga agtctccgcg agaactcgct
     2401 ttcggagcag gtcgcccgcg ccgcgggtga ccttgccccg gagggcgtgg aagtcgggat
     2461 ctatgaaggc ctggctt
//
LOCUS       DAOQMF010000236         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26222_length_2474_cov_4.250930, whole genome shotgun sequence.
ACCESSION   DAOQMF010000236 DAOQMF010000000
VERSION     DAOQMF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26222_length_2474_cov_4.250930"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /gene="cydD"
                     /locus_tag="PLV77_04905"
     CDS             <1..1225
                     /gene="cydD"
                     /locus_tag="PLV77_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HNC15216.1"
                     /translation="LAWVLRYNWEAAAILAVTAPLIIVFMVLIGLLTEQRTRKRWERM
                     SRLSSHFLDLVGGLATLRANSQAYSQSGSIQAVGEDYRRETMGALRVGFLSSLVLELL
                     AMIGVAMVATAVGIQLAGGHLELVAGLTVLLLAPELYMPLRQLGAQFHASADGMAAAE
                     RIFEVIDQPAVVSTPAEPVPAPDPARWSVAMEGVSYAWPGRERVLESIDLELKPGETV
                     ALVGPSGGGKSTLASLLLRLTEPGSGRISCGGTDLSEVDPQSWRRQIAWVPQRPTVFA
                     DTLAANLRLFNPQATDAQLAEAIGVAVLGEVVRGLPDGLETMVGEGGRRLSAGQTQRI
                     ALARALLSEAPLLILDEPTAHLDQDTEKELAEAINEAASGRTALIISHREQPIARADR
                     TLLLNQGRLGEMVNA"
     gene            1222..>2474
                     /gene="cydC"
                     /locus_tag="PLV77_04910"
     CDS             1222..>2474
                     /gene="cydC"
                     /locus_tag="PLV77_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HNC15217.1"
                     /translation="MIAARLERTPLGRAALATRNRWKLFTISVLLGTGTVLAAVGLLT
                     ASGYLISRAAERPEILSLTVAIVGVRFFGVSRALLRYGERLTSHDLAFRTLTDLRGRF
                     FERLIPLVPAGLDGSRRADLMSRFVGDVDRLQDLYLRALSPPLVAIFCSVFCVALASI
                     ILPVAGLVLAVMLLLGGLAAPALTRWAARTAGRRQSRARGLLAMDLHEIAVGGAEIAV
                     AGRESDWLERSREDDRHLVSLQRRDAMSGGLAEGLGTLLAVGAAVAVTAASIPAVHSG
                     ELNGVLLAALALLAMASFEAITPLSQAAAVIDATDEAAERIEEVSERPVPILNPEHPR
                     PVPAGGPIRLDGVSFAYPGGDGLLLRDADLEIRPGEAVALTGPSGIGKSTLSELLVRF
                     RDPDRGRITLGGADIRDLDQEELREA"
BASE COUNT          358 a          831 c          862 g          423 t
ORIGIN      
        1 gctggcctgg gtgctccgtt acaactggga agcggccgcg atcctcgcgg ttacggcgcc
       61 gctgatcatc gtcttcatgg tcctgatcgg actgctgacc gagcagcgca cccgcaagcg
      121 ctgggagcgg atgtcccggc tgtcgtcgca tttcctggac ctggtcggcg ggctggcgac
      181 cttgcgggcc aacagccagg cctactcgca atcgggctcg atccaggcgg tcggggagga
      241 ctatcgccgg gagaccatgg gggctctccg ggtcggcttc ctttcctcgc tggtccttga
      301 acttctggcg atgatcgggg tggcgatggt cgcgaccgcg gtcggaatcc agctcgccgg
      361 cggccatctt gaactggtcg ccgggctgac cgtgctgctg ctcgcccccg agctttacat
      421 gcccctgcgc cagctcggcg cgcagtttca cgccagtgcc gacggcatgg cggcagccga
      481 gcgaattttc gaggtgatcg accagccggc agtggtgtcc acgccggctg aaccagtacc
      541 cgctcccgac ccggcccgct ggtcggttgc catggagggc gtttcatacg cgtggccggg
      601 tcgggagcgg gtgctggagt cgatcgacct cgaactgaag cccggcgaaa cggtggcgct
      661 ggtcggcccg agcggtggcg gcaagagcac cctggcttca ctacttctgc ggctgactga
      721 accgggttcc ggccggatca gctgtggcgg cactgacctg tctgaagtcg atccgcagag
      781 ctggcggcgg cagatcgcct gggtgcccca aaggccgacc gtcttcgccg acacactggc
      841 ggcgaacctg aggttgttca atccgcaggc caccgacgcc cagctggccg aagcaatcgg
      901 ggtggcggtg ctgggtgaag tggtcagggg gctgcccgac ggcctggaaa ccatggtcgg
      961 cgaaggaggc cggcggctgt cggctggtca aacccagaga atcgccctcg ccagggccct
     1021 tttgagcgag gcccctctgc tgatcctcga tgaaccgacc gctcacctcg accaggacac
     1081 cgagaaggaa ctcgcggaag cgataaatga agccgcgagt ggccggaccg ccctgatcat
     1141 ctcccatcgc gagcagccga tcgcgagggc cgaccggacc ctgctgctga accaggggcg
     1201 actgggtgaa atggtgaacg catgatcgcc gcccggctcg aacggacccc gctgggccga
     1261 gccgccctcg cgaccaggaa tcgctggaag ctgttcacga tttcggtgct gctcggtacg
     1321 ggcacggtgc tggcagcggt gggtctgctg accgcctccg gctacctgat cagccgtgcc
     1381 gccgagcggc cggagatcct ctcgctcacc gtggcgatcg tcggggtccg cttcttcggg
     1441 gtctcccggg ccctgcttcg ctacggcgag cgcctgacct cgcatgacct ggccttcaga
     1501 accctcaccg acctgcgcgg ccggttcttc gaacgcctga tcccgctggt cccggccggg
     1561 ctcgacggct cgcggcgggc cgatctgatg agccgtttcg tcggtgacgt cgaccgcctc
     1621 caggatctgt atctgcgggc cctttcgccg ccgctggtcg cgatcttctg ctcggtcttc
     1681 tgcgtggcgc tcgcctcgat catcctgccg gtggccgggc tggtgctggc cgtgatgctg
     1741 ctgctcggtg gtctcgcggc tccggccctg acccgctggg cggcacggac ggccggccgc
     1801 cgccagtcca gggcaagggg ccttctggcc atggaccttc atgagattgc cgtcggcgga
     1861 gccgagatcg ccgtggccgg tcgcgaaagc gactggctcg agagaagccg ggaggatgac
     1921 cgccacctcg tttcactcca gcggcgggac gcgatgagcg gcggtctggc ggaaggtctc
     1981 ggaaccctgc tggccgtagg cgccgcggtt gccgtcaccg ccgcctcgat tccggctgtc
     2041 cattcgggtg agctgaacgg cgtgctgctg gccgccctgg ctctgctggc aatggcctcc
     2101 ttcgaggcga tcaccccgct cagccaggcg gcggccgtga ttgacgcgac cgacgaagcg
     2161 gcggaacgca tcgaggaagt gtccgagcgt ccggtaccca tcctcaaccc tgaacatccc
     2221 agaccggtgc cggccggcgg cccgatcagg ctggacggcg tgagtttcgc ctacccggga
     2281 ggggacggcc tgctgctgcg cgacgccgat ctggaaatcc ggcccggcga ggcggtcgcc
     2341 ctgaccgggc ccagcgggat cggcaagagc accctttccg aactcctcgt tcgcttcagg
     2401 gatccggacc gtggacgaat aacgctcggt ggcgcggaca tccgtgacct ggaccaggag
     2461 gagttgcgcg aagc
//
LOCUS       DAOQMF010000237         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26346_length_2468_cov_3.917944, whole genome shotgun sequence.
ACCESSION   DAOQMF010000237 DAOQMF010000000
VERSION     DAOQMF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26346_length_2468_cov_3.917944"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLV77_04915"
     CDS             complement(<1..379)
                     /locus_tag="PLV77_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNC15218.1"
                     /translation="MTERIDLDREGPRDECGVFGVYAPGHDVSRLAYFSLYALQHRGQ
                     ESAGIATCEGGHITTMRDGGLVSQVFDEDKLRALTGDMAIGHVRYSTTGGGSWENAQP
                     IWRDDGRELALAHNGNLTNAVEIY"
     gene            537..1553
                     /locus_tag="PLV77_04920"
     CDS             537..1553
                     /locus_tag="PLV77_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15219.1"
                     /translation="MAVMRGQRGLGLLVVGAILASALVLLPATSPAATKKQKQCQRAI
                     ATVFTFKKGVPKKKRRKAVKIICQTMTVGKQGPKGAKGDPGARGATGATGPKGATGSA
                     NGVTGPVGPTGATGQKGATGAIGLPGITGATGATGFQGLIGPTGPIGLPGLTGATGAT
                     GLIGEIGPTGPTGLTGLIGPTGPTGLTGAVPILISDIPSFSAQAMSDVINPITNWNED
                     YDSTNSFNPVTGVFTAPTTGSYLIEPDITTGPASEVVAGGGQPPLVAVNVNNVDVDVQ
                     AFPLFNVDINLILALDVPLQTAQASPTTFQQLDAGDQVSIQIIKQNGVVYDTYGDLMI
                     TRLP"
     gene            complement(1556..2356)
                     /locus_tag="PLV77_04925"
     CDS             complement(1556..2356)
                     /locus_tag="PLV77_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC15220.1"
                     /translation="MLPRSERTRIGEMPVGIGKGQTVGGAALSGIGFTVSLLIARLAF
                     DDQQTQDHAVVGIFLASIISVALAWTIFKLAAVFRGEVSAGLPRKLSLPFDPDRDHFL
                     GSPDAPFVLVEYGDYECPFCGRATGMVRELRERMGDQLLYVYRHLALVDVHPHAELAA
                     EAAEAAGEQGKFWEMHVELYDHQGDLDIEDLIGYAAKLDMDVDRFVEDVQAGRLARRV
                     QQDAASGDASGAHGTPTFFVNGVRHVGAYDAVSLAAELRAAEPVPAGA"
BASE COUNT          444 a          799 c          836 g          389 t
ORIGIN      
        1 tgtagatctc gaccgcgttg gtcaggttgc cgttgtgggc gagggcgagt tcgcgaccgt
       61 cgtcacgcca gatcggctgg gcgttttccc atgagccgcc gccggtggtc gagtaccgga
      121 cgtggccgat cgccatgtca ccggtcaggg cccgcagctt gtcctcgtcg aagacctggg
      181 agaccaggcc gccgtcccgc atggtcgtta tgtgaccgcc ttcgcaggtc gcgatcccgg
      241 ccgattcctg gccacggtgc tgaagcgcgt acaggctgaa gtacgccaaa cgggacacgt
      301 cgtgtccggg ggcatacacg ccgaagacac cacactcgtc gcgtggtccc tcgcggtcca
      361 ggtcgatccg ctcagtcaca ggtcaggcca ccaagaatgg aaaaggacaa aggtgcaggc
      421 agtctgcctg cattggcaga ttagcagcgc cccccggttt gcttgccgat tccccacaaa
      481 acagccgcaa atcgcgggtt tggcagccgt cgagccgttc gatgtagagt ggatgcgtgg
      541 ccgtgatgcg gggacagcgg ggactgggtc tgttggtggt cggagcgatt ctcgcctcgg
      601 cgctggtcct cttgcccgca acctccccgg cggcgaccaa gaaacagaag caatgccagc
      661 gggcgatcgc gaccgtcttc accttcaaga agggcgtgcc gaagaagaag cgccggaagg
      721 ccgtgaagat catctgccag acgatgacgg tcggcaagca gggaccgaag ggcgcgaagg
      781 gtgatcccgg ggccaggggc gccaccggag cgaccgggcc taaaggggca accggatcgg
      841 cgaacggtgt gaccggaccc gtcgggccga ccggagcgac gggtcagaag ggtgcgaccg
      901 gggcgatcgg tttgccggga atcaccgggg cgaccggcgc caccggcttt cagggattga
      961 tcgggccgac cggaccgatc gggctgcccg gactgaccgg ggcaaccggc gcgactggtt
     1021 tgatcggtga gatcgggccg accgggccca ccggactgac cggtctcatc gggccgaccg
     1081 ggccgaccgg cctgacgggc gccgtgccga tcctcatctc ggacatcccg tctttctcgg
     1141 cccaggcgat gtccgacgtg atcaacccga tcaccaactg gaacgaggat tacgactcga
     1201 cgaattcctt caaccctgtc acgggggtgt ttaccgcccc gacgaccggc tcgtacctga
     1261 tcgaaccgga catcacgacc ggaccggcgt ccgaggtggt tgccggcggc ggccagccac
     1321 ccctggttgc cgtcaacgtg aacaacgtcg acgtggacgt gcaggcgttc cccctgttca
     1381 acgtcgacat caacctgatt ctcgccctgg acgttcccct ccagacagca caggcatcgc
     1441 cgaccacctt ccagcagctc gacgcggggg atcaggtctc gatccagatc atcaagcaga
     1501 acggcgtggt gtacgacacc tacggcgacc tgatgatcac caggctgccg taacttcagg
     1561 ccccggccgg aacgggttcg gcggcgcgga gctccgcggc gaggctgacc gcgtcatacg
     1621 cgccgacgtg acggaccccg ttgacgaaga aggtcggggt gccgtgggca cccgaggcgt
     1681 caccagaggc ggcgtcctgc tggacccgac gggcgaggcg cccggcctgg acatcctcga
     1741 cgaagcggtc gacgtccatg tccagcttcg cggcgtatcc gatcaggtct tcgatgtcga
     1801 ggtcgccctg atggtcgtag agctcgacat gcatttccca gaacttcccc tgctccccgg
     1861 ctgcctccgc cgcctcggcc gcgagttcgg cgtgcggatg cacgtcaacc agcgccaggt
     1921 gccggtacac gtagagcagc tggtcgccca tccgctcgcg aagttcccgg accattccgg
     1981 ttgcccggcc gcagaatgga cattcgtaat cgccgtactc gaccagcaca aagggcgcgt
     2041 cgggactgcc gaggaaatgg tcgcgatccg gatcgaaagg caggctgagc ttgcgaggca
     2101 ggccggcact gacctcgccg cggaagaccg ccgcgagctt gaagatcgtc caggccaggg
     2161 cgacggaaat tatcgaggcg aggaagatgc cgaccaccgc atgatcctgg gtctgctggt
     2221 catcgaaggc gagccgggcg atcagcaggg aaaccgtgaa gccgatcccg gagagggccg
     2281 cgccgccgac cgtctggcct ttgccgatcc cgaccggcat ctcgccgatc ctggtccgtt
     2341 cgcttcgcgg cagcattctc gaggcgctgg aggcggatta tgtacgcacc gcgcgggcga
     2401 agggcctggc ggaccgcatg gtggtcatgg ggcacgtgct caagaacgcg ctgattccgg
     2461 cggtgacg
//
LOCUS       DAOQMF010000238         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26719_length_2449_cov_3.258981, whole genome shotgun sequence.
ACCESSION   DAOQMF010000238 DAOQMF010000000
VERSION     DAOQMF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26719_length_2449_cov_3.258981"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PLV77_04930"
     CDS             complement(<1..1317)
                     /locus_tag="PLV77_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15221.1"
                     /translation="MSVYLDMTNNNSIGEKAMSLSGSGTETGRIGRMVLFLTILAAVF
                     SLGHSAAQGGTFSWSEAKTVDQAPFRNSGGIKSVSCPSTSFCMAVDENQRMLVSHNPF
                     ATSPEWETKQTSDRALRFQMISCPSTSLCVAVAAKTEYDPQTHSVWYALVSTDPTSSE
                     PHWAPEQIFNQPGENIDAISCVSASLCVAVTSLGKALISTNPTAASPVWTTYTVAANQ
                     YLDAVSCPTTGLCVTKAGGGGQVFTSTNPTSSSVTWRTKNLEVGYPNEALTDVTCASE
                     SLCAATTTNGSFRVTTNPTATNPLWSKTFASDSFGFGAAVTCTSVSLCAVVDGVGGLH
                     ITRNPTEPTPGWSEHDPGYGSLLSRSGSITCPSNNACMAFGANGKDFYPLTGLSTDSA
                     GWTASDIWNDHHLPTGNPSCPSTTFCATVDNGDFLRTSNPYGATPNW"
     gene            complement(1375..1722)
                     /locus_tag="PLV77_04935"
     CDS             complement(1375..1722)
                     /locus_tag="PLV77_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15222.1"
                     /translation="MANWKLTVRHGPDVKRQSFDDLDDALGAARKAADEVIAEGPLDS
                     VSAIRDYEPGQIVNARIEISGKGFFSPPTAGFDIQGDLSVIAYAGGVKRQTLDGSTVA
                     QAIKSLLDYLGTR"
     gene            complement(1761..>2449)
                     /locus_tag="PLV77_04940"
     CDS             complement(1761..>2449)
                     /locus_tag="PLV77_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNC15223.1"
                     /translation="PDPRLSKAIDVLFILHADHEQNCSTSAVRGVGSSDVDPYSAVAA
                     GIAALYGRLHGGANEAVLQMLRRIEKVDNIPGFLEAVKNREEKLMGFGHRVYKNYDPR
                     ARIIKSHADEVFEATEYNPLVEIATELEKQALDDEYFTSRKLFPNVDFYSGIIYEALR
                     IPPGMFTVIFAIARTAGWISQWLEMTDDPDKKIARPRQIYTGAREANYVPVGERTGED
                     RISGPAVRPS"
BASE COUNT          452 a          699 c          805 g          493 t
ORIGIN      
        1 ccagtttgga gtggctccgt aagggttcga ggtccgcagg aagtctccat tgtcgactgt
       61 cgcgcagaag gtcgtgctgg ggcaagaggg gttgccggtt ggcaggtggt ggtcgttcca
      121 gatgtcggaa gcggtccaac ccgcgctatc ggtgctcaaa ccggtcaggg gatagaaatc
      181 cttgccgttg gcaccaaacg ccatgcaagc gttatttgag gggcaggtga tgctgcccga
      241 tcttgaaagg aggctcccgt acccgggatc gtgctcggac caacctggcg tcggttcggt
      301 ggggttcctc gttatgtgga gtcccccgac tccatcgacc acggcgcaaa gactcaccga
      361 tgtgcaggtc acggcggcac cgaagccgaa actgtcgctg gcgaaggtct tggaccacaa
      421 tgggtttgtg gcggtgggat tcgtcgtaac ccggaaactc ccatttgttg tcgtcgcggc
      481 acacaatgac tccgaagcac aggtcacgtc ggtgagcgct tcattggggt agcccacctc
      541 gaggttcttg gtcctccagg tgacgctgga cgatgtcggg ttggttgacg tgaacacctg
      601 gccgccccca cccgctttgg ttacgcaaag cccggtggtc gggcaagaga cggcgtccaa
      661 gtactggttt gcggcaacgg tgtatgtcgt ccagaccggc gatgcggccg tcggattggt
      721 tgagatcagg gcctttccca ggctggtgac cgcgacacaa agagacgccg acacacagga
      781 gatcgcgtcg atgttctcgc cgggctggtt gaagatctgt tcgggagccc agtggggctc
      841 gctcgaggtc ggatcggtgc tgaccagtgc gtaccaaacg gagtgggtct gggggtcata
      901 ttcagtcttg gctgccaccg caacgcaaag ggacgtcgac gggcacgaaa tcatttgaaa
      961 ccggagagca cggtccgagg tttgcttagt ttcccactca ggcgaggtgg cgaacgggtt
     1021 atgggaaacg agcattcgct ggttttcatc taccgccata cagaaggagg tcgacgggca
     1081 ggaaacggac ttgatgccgc cagaattccg gaacggtgcc tgatcgacgg tctttgcttc
     1141 ggaccatgaa aacgttccac cctgagcggc ggagtgtccc aaactgaaca cggcggcaag
     1201 gatggtcaag aaaaggacca tccgcccgat gcgtccggtt tcagtgcctg aacctgaaag
     1261 actcattgcc ttctccccta ttgaattgtt gttcgtcatg tccaaataga cggacaacct
     1321 caccagcatc ttcctcgccc ggctgattta tttcaagtca gaccgagcga tgacctagcg
     1381 agtacccaag tagtcgagca gggatttgat tgcttgagcg accgtcgacc cgtcgagggt
     1441 ctgccgcttg acccctcccg cgtaagcgat gaccgagagg tcaccctgga tgtcgaagcc
     1501 ggcggttggc ggagaaaaga aacccttgcc gctgatttcg atgcgggcgt tgactatctg
     1561 gcccggctcg tagtcacgga tcgcgctgac cgaatcgagc gggccttcgg ctatgacctc
     1621 atcagcggcc ttgcgggcag caccgagggc atcgtcgaga tcgtcgaagc tctgacgctt
     1681 gacatcaggg ccatgccgga ccgtcagctt ccagttggcc actgggctca ctcagtccgg
     1741 gattgcgctc gacaggcgac tcaggagggg cggacggccg ggccggaaat ccggtcctcg
     1801 ccggtgcgct cgcctacggg cacgtaattt gcctcgcggg cgccggtgta gatctggcgc
     1861 ggccgggcga tcttcttgtc cgggtcgtcg gtcatctcca gccactggga gatccacccg
     1921 gcggtgcggg cgatcgcgaa gatcaccgtg aacatgcccg gcgggatccg cagggcctcg
     1981 tagatgatcc cggaatagaa gtcgacgttc gggaagagct tgcgcgaagt gaagtactcg
     2041 tcgtcgagag cctgcttttc gagctcggtc gcgatctcga ccagcgggtt gtactcggtc
     2101 gcctcgaaga cttcgtcggc gtggctcttg atgatcctcg cccgcgggtc gtaattcttg
     2161 tagacccggt ggccgaagcc catcagcttc tcttcgcggt tcttgaccgc ctcgaggaag
     2221 ccggggatgt tgtccacctt ctcgatccgg cgcagcatct gcagcaccgc ctcgttggcg
     2281 ccgccgtgga ggcggccgta tagggcggcg atgccggcgg cgacggccga gtacgggtcg
     2341 acatccgagg aaccgactcc gcgcacggcg ctggtcgagc agttctgctc gtggtcggcg
     2401 tgcaggatga agagcacgtc gatcgccttc gaaaggcggg ggtccggtt
//
LOCUS       DAOQMF010000239         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26747_length_2448_cov_2.448391, whole genome shotgun sequence.
ACCESSION   DAOQMF010000239 DAOQMF010000000
VERSION     DAOQMF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26747_length_2448_cov_2.448391"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLV77_04945"
     CDS             complement(<1..582)
                     /locus_tag="PLV77_04945"
                     /inference="COORDINATES: protein motif:HMM:NF028194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNC15224.1"
                     /translation="MSTENRRYALAERPEGLIDEDTFTLETGPVPEIGEGEALVRVTW
                     ISIDPTNRTWIGEEPTYLPPVQIGETMRALGLGEVVESNNPNYPVGALVTGLTGWTDY
                     TVISDSNPLMVVPEDARSAPPAALLGMLGMTGCTAYFGMLEIGEPKEGETVVVSAAAG
                     AVGTVAGQLARLKGARVVGIAGGPEKCAWLIDEL"
     gene            666..2168
                     /locus_tag="PLV77_04950"
     CDS             666..2168
                     /locus_tag="PLV77_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNC15225.1"
                     /translation="MAAGLAAANALAPTGPFGAAQALADPKRRLRRPGFGLAFKSLKD
                     ETFLPSIDLDGKLPQWLTGSLLRNGPALFEIGEEKFNHWFDGLAMLHAFSFKGGKVSY
                     RNRFLRSSQYWAWQREGRIKFSELATDPAPDPCRQIFSGVSTLPVLGRIPNANVSIEK
                     LAGEFRAHTEIPIPVRFNPKFLNTMGIGDAGQMQGRLGTAHPHFDPETRERFSYEVEL
                     VPPSGLRIVSEKGRTRRELAFIPQAMPGYLHSFGLTRRFVVIVSQPFNFNLSKFLSPD
                     RGPIITNYEWQGEEPTRIILVDRQRGGVAHTVETDSFFAFHHINAYEHEGKVIVDVCA
                     HKDAGVIDALYLKKIRSGSKVPQARYRRFEIRLEGQGKLSQRDLIDAYIELPQKNYGR
                     VNGRHYRYAYGVGLRKGSSGFIDQLVKADVKIGESKLWREWGVYPGEPVFVPRPGGKA
                     EDDGVILSVILDGRRRKSALLVLDARNMEEIARASVPHHIPFGFHGLYAS"
BASE COUNT          433 a          792 c          781 g          442 t
ORIGIN      
        1 gagctcgtcg atgagccagg cgcatttctc cggaccgccg gcgatcccga ccacccgggc
       61 gcccttcagc ctggcgagct ggccggccac ggtgccgacc gctcctgccg cggcggaaac
      121 gacgaccgtc tcgccttcct tcggctcgcc gatctcgagc atcccgaagt aggcggtgca
      181 gccggtcatg ccgagcatgc cgagcagggc ggcgggagga gcggacctgg cgtcttccgg
      241 caccaccatg agcggattgg agtcactgat cacggtgtaa tcggtccagc cggtcaggcc
      301 ggtgaccagg gcgccgaccg ggtaattcgg attgttcgat tcgaccactt cgccgaggcc
      361 cagggcccgc atcgtctcgc cgatctggac cgggggcagg taggtcggtt cttcaccgat
      421 ccaggtccgg ttggtcgggt cgatcgagat ccaggtgacc cggaccaggg cctcgccctc
      481 gccgatctcc gggaccggcc ccgtctccag ggtgaaggtg tcctcgtcaa tcaggccttc
      541 gggccgctcc gccaatgcat aacgccgatt ctccgtgctc atatagaaga acctatagtg
      601 gagcaaggag agagggagaa gctgtgggaa aaacgcgtag agacttgctc gttacgggtt
      661 cgagagtggc ggccggactg gcggcggcca acgcgcttgc gccgaccgga cccttcgggg
      721 cagcgcaggc gttggctgat ccgaagagaa ggctgcgccg gcccggtttc ggcctcgcat
      781 tcaagtcgct gaaggacgag accttcctgc cctccatcga tcttgacggc aagttgccgc
      841 agtggttgac cggcagtctg ctgcgcaacg gcccggccct cttcgagatc ggcgaggaaa
      901 agttcaacca ctggttcgac ggcctcgcga tgctccacgc cttctccttc aagggcggca
      961 aggtcagcta ccgcaaccgc ttcctgcgct ccagccagta ctgggcctgg cagcgtgaag
     1021 gccggatcaa gttctccgag ctggccaccg acccggcccc ggacccctgc cgccagatct
     1081 tctccggggt ctcgaccctg ccggtgctgg gccggatccc caacgcaaac gtctcgatcg
     1141 agaaactggc gggcgagttc cgggcccaca ccgaaatccc gatcccggtc cgcttcaacc
     1201 cgaaattcct gaacacgatg gggatcggtg acgccgggca gatgcagggt cgcctcggaa
     1261 cggcccatcc ccacttcgac ccggaaaccc gggaacgatt ctcctacgag gtcgaactgg
     1321 tgccgccgag cgggctccgg atcgtctccg aaaaaggcag aacccggcgg gagctcgcct
     1381 tcatcccgca ggcgatgccg ggttaccttc actccttcgg tttgacccgg cggttcgtgg
     1441 tgattgtctc ccagcccttc aacttcaacc tctcgaaatt cctcagcccg gaccgcggcc
     1501 cgatcatcac caactacgaa tggcagggcg aggagccgac ccggatcatc ctggtcgacc
     1561 gccagagggg tggtgtggcc cacacggtcg agaccgactc cttcttcgcc ttccatcaca
     1621 tcaacgccta cgagcatgag ggcaaggtga tcgtggacgt ctgcgcccac aaggacgccg
     1681 gggtgatcga cgcgctctac ctgaagaaga tccgttccgg cagcaaggtg ccgcaggcca
     1741 ggtatcgccg gttcgagatc agacttgaag gtcaggggaa gctgagccag cgcgacctga
     1801 tcgacgccta catcgagctg ccgcagaaga actacggccg ggtcaacggc aggcattatc
     1861 gctacgccta cggggtcggt ctgcgcaagg gatcgagcgg cttcatcgac cagctggtca
     1921 aggccgacgt gaagataggc gagtcaaagc tctggcgcga atggggggta tacccgggcg
     1981 aaccggtatt cgtgccgagg cccggaggga aggcggaaga cgacggggtg atcctctcgg
     2041 tcatcctcga cggccggcgg cgaaagtcgg ctttgttggt cctcgacgcg cggaacatgg
     2101 aggaaattgc gcgtgcttca gtgccgcatc acatcccctt tggcttccac gggctgtatg
     2161 cttcgtgaac aggggtgttt acatttgtag ttgacgggta cttccgtact caggcctttg
     2221 gaggactgat ggagcgcgtc ccgccacaac agaacacagg cagtctcccg ctggtgatcg
     2281 ggcttaccgc gctggtcttg gtcgtcccct gcgccgcggc gattgtgctc agggccaccg
     2341 gaatcgtcac ctcgccggtg ttgctgatcc tgatcccggt gctggtttcc gttctctttt
     2401 cccattcgat ttccgagttc tggaagcggc gccgggccgg cagtcccc
//
LOCUS       DAOQMF010000240         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26929_length_2438_cov_2.688208, whole genome shotgun sequence.
ACCESSION   DAOQMF010000240 DAOQMF010000000
VERSION     DAOQMF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26929_length_2438_cov_2.688208"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..758
                     /locus_tag="PLV77_04955"
     CDS             210..758
                     /locus_tag="PLV77_04955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNC15226.1"
                     /translation="MKPGPGNARERAAEIAVEKPGISRSLTLDWQDPTASAAEGSKLA
                     GLEYMVAVMDGTIPPAPIAVLMNMTPTEVEEGRAVFFALPGEEHYNPIGVVHGGFMAT
                     LLDSVAGCAVHTTLALGEAYTTLTLEVKNLRALTRDTGSVRAEGEVTYRGRRQATADA
                     KLYAEESGKLLASATSTCLIMA"
     gene            complement(765..2210)
                     /locus_tag="PLV77_04960"
     CDS             complement(765..2210)
                     /locus_tag="PLV77_04960"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNC15227.1"
                     /translation="MKAYTTDLALSDLDADLVAVGLFEGDELSEPLGSTAGASDASAD
                     FKSKVIVYPGKPERVVIIGLGPKDGFTLEKARVAGAVAQKALKEVKGEALAWMLPDTG
                     DLDPGGVAACLVEAAIFSAFDFDRYKSGSDGKEAPPSLKAVEINTGKDVAAMVKVGEA
                     VGNAGNRARYLQSLPANVATPEYLAGRAREIAEAHEKVTVEVMDRAAIIKAGMGGLEA
                     VSKGGEHVEPRLITLRYTGRGGGETLGLVGKSVTFDTGGISIKPSAGMHEMKMDMSGG
                     AAVLEGLNAIAELEIPLDIIAVLPATENMPSGTALKPGDIITQLNGKTVEVINTDAEG
                     RLILADALVHCAREGADRLVDLATLTGAVLIGLGSTYAALISNDDDLAAAVEKAGELS
                     GELVWRLPLHDEYKDLTKGEITDLVNASAQRKAGTIYAGSFLEEFTEGKPWAHLDIAG
                     TAWDTGREYWGKGPTGFGVHLLVALAYDLSS"
     gene            complement(2243..>2438)
                     /locus_tag="PLV77_04965"
     CDS             complement(2243..>2438)
                     /locus_tag="PLV77_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15228.1"
                     /translation="LRKVTGSLKADGSKTIILRLARKQANRPLLARIKQTRKVRALVM
                     VTLSDQAGNRKRIRLPVKLR"
BASE COUNT          392 a          805 c          827 g          414 t
ORIGIN      
        1 ggtggactgc agacgcgacc ctctacggag ggccgaaccg accccgaaag gggagcaaga
       61 atgaagcgca atccgagaaa ccgaacgaga ttccagatga ggcccctgaa gatcgacctc
      121 acagaggcag agatcctcgc cgacctgcag tacggggttg gcgggaagat caggccggag
      181 ggcgcacccc gacgctggag gccgacccgg tgaaacccgg ccccggtaac gcgagggagc
      241 gggcggcgga gatcgcggtt gagaagcccg ggatcagccg ttcgctcacc ctcgactggc
      301 aggacccaac ggcctcggcg gcggaaggca gcaagctggc cggcctcgaa tacatggtcg
      361 cggtcatgga cggaaccatt ccacccgcgc cgattgcggt gctcatgaac atgacaccga
      421 ccgaggttga agagggccgc gccgtgttct tcgccctgcc gggggaggag cactacaacc
      481 cgatcggcgt cgttcacggc ggcttcatgg cgaccctgct cgattcagtc gcgggttgcg
      541 cagttcacac gacgctggcg ctgggcgagg cctacacgac tctcaccctg gaagtgaaga
      601 acctgcgtgc gctgacccgc gacacgggca gcgtgcgggc cgagggtgag gtcacctacc
      661 ggggccggcg gcaggcgact gccgacgcga agctctacgc cgaggaaagt ggcaagctgc
      721 tcgcgtcagc cacctcgact tgtctgatca tggcgtgacc cggatcagct gctgaggtcg
      781 tacgcgaggg cgaccagcag gtgaacgccg aaaccggtcg ggcccttgcc ccagtactcg
      841 cggccggtgt cccaggcggt gccggcgatg tccaggtggg cccagggctt gccctcggtg
      901 aactcctcga ggaatgatcc ggcgtagatc gtgccggcct tgcgctgggc ggaggcgttg
      961 accagatcgg tgatctcgcc cttggtcagg tccttgtact cgtcgtgcag cggcaggcgc
     1021 cagaccagtt cgccactcag ctcgcccgcc ttctcgacgg ctgcggcgag gtcgtcgtca
     1081 ttcgagatca gggccgcgta ggtcgagccg aggccgatca gcaccgcgcc ggtcagcgtc
     1141 gcaaggtcga ccaggcggtc ggcgccttcg cgggcgcagt ggaccagggc atcggccagg
     1201 atcagccggc cctcggcgtc ggtgttgata acctcgaccg tcttgccatt gagctgggtg
     1261 atgatgtcgc cgggcttcag ggcggttccg gacggcatgt tctcggtcgc cgggaggacg
     1321 gcgatgatgt cgagcggaat ctccagctcg gcaatcgcgt tcaggccctc gagcaccgcg
     1381 gcgccgccgg acatgtccat cttcatctcg tgcattccgg ccgagggctt gatcgagatg
     1441 ccgccggtgt cgaaggtgac cgacttgccg accaggccga gcgtctcgcc cccgcccctg
     1501 ccggtgtacc ggagagtgat caggcgcggc tcgacatgct cgccgccctt ggaaacggcc
     1561 tcgaggccac ccatgccggc cttgatgatc gccgcgcggt ccattacttc gaccgttacc
     1621 ttctcgtgcg cttcggcgat ctcgcgggcc cggccggcca ggtactcggg ggtcgccacg
     1681 ttggccggca ggctctgcag gtagcgggcc cggtttcccg cgttgccgac ggcttccccg
     1741 accttgacca tcgcggccac gtccttgccg gtgttgatct cgacggcctt cagtgagggc
     1801 ggggcctcct tgccgtcact tcccgacttg tagcggtcga agtcaaaggc gctgaagatc
     1861 gccgcttcga ccaggcaggc ggcgaccccg cccgggtcga gatcaccggt gtcgggcagc
     1921 atccaggcca gtgcttcgcc cttgacttcc ttcaaagcct tctgggcgac cgctccggcc
     1981 acccgggcct tctccagtgt gaacccgtcc ttcgggccga ggccaatgat caccacccgc
     2041 tccggctttc ccgggtagac gatcaccttc gacttgaagt cagcggaagc gtccgaggcc
     2101 ccggccgtgc tgccgagcgg ctcggaaagc tcgtcacctt cgaagaggcc gaccgcgacg
     2161 aggtcggcgt cgagatcaga cagggcgaga tcggtcgtgt aagccttcat tcgccggagt
     2221 ctaagacccg cgggtgcggc ggtcagcgga gcttgaccgg gaggcggatc cgcttcctgt
     2281 tcccggcctg gtcgctcagc gtgaccatga ccagcgcccg gacctttcgg gtctgtttga
     2341 tccgggcaag cagcggccgg tttgcctgtt tcctggccag gcgcaggatg atcgtctttg
     2401 aaccgtccgc tttcagggat ccggtcacct ttctcagg
//
LOCUS       DAOQMF010000241         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26931_length_2438_cov_2.515317, whole genome shotgun sequence.
ACCESSION   DAOQMF010000241 DAOQMF010000000
VERSION     DAOQMF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26931_length_2438_cov_2.515317"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLV77_04970"
     CDS             complement(<1..858)
                     /locus_tag="PLV77_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC15229.1"
                     /translation="MAEKKSSGDASYGAKDITVLEGLEAVRKRPGMYIGSTGPRGLHH
                     LVYEIVDNSVDEALAGHNESVEVNLHPDNSCTVTDHGRGIPVDIMEKEGRPAAEVVLT
                     VLHAGGKFGDGGGYKVSGGLHGVGSSVVNALSSWLELRICRDGFVWNQRYERGRPVTE
                     LEKGEATKETGTQITFLPDDEIFEELDFDFQTLLERLRETAFLTRGLRIQITDERAEG
                     QEVEFKYDGGIEDFVRYLNENKEPLGKKVVYFEGEDEKEGQVEVAMQWNASYNDSIHS
                     FANNINSHEG"
     gene            complement(1053..1361)
                     /locus_tag="PLV77_04975"
     CDS             complement(1053..1361)
                     /locus_tag="PLV77_04975"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNC15230.1"
                     /translation="MRDARGRSPSPKRLGEVLGDFRGEVTPPTLLAAVQTQWDEVVGP
                     RIAGVTDVIDEREGVVTVECSSAVWAQELELMAPRILARLKDRIDGPVPEKLRFRAAR
                     "
     gene            complement(1358..>2438)
                     /gene="recF"
                     /locus_tag="PLV77_04980"
     CDS             complement(1358..>2438)
                     /gene="recF"
                     /locus_tag="PLV77_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNC15231.1"
                     /translation="FEPDPGLTVIIGPNGAGKTTLVEAIYFGLTSRSFRTSDRRDLIP
                     FGSAFARCRVTVQGGGVDHVFMASASRSEGNRFTMDGARIEQAEAVLHRPKVTVFSPD
                     RLELVKGPPAGRRAHLDSFVAARWPSRAGLRSTFGRILAQRNALIARISSGEANSSQL
                     ATWNSQFAEAGANLASGREAAVEALAERWVEAADDLGLEGPKGISYRPGSATTKEEIE
                     AGLEERLEHDLRLGRSSWGPHHDEVRLELDGRQLRRFGSQGQQRLGLHALLFAERAAL
                     LDAGEAAPLMLLDDVMSELDAHRRGRLVERLLGGGQSVITAAEGELIPERQEISRVAI
                     RDLVGADEERDNGEDPGAGSGGGTS"
BASE COUNT          389 a          840 c          764 g          445 t
ORIGIN      
        1 accctcgtgg gagttgatgt tgttggcgaa gctgtggatc gaatcgttgt aggacgcgtt
       61 ccactgcatc gccacctcga cctgaccctc tttttcatcc tcgccctcga agtacacgac
      121 cttcttgccg agcggctcct tgttttcgtt caggtagcgg acgaagtcct cgatgccgcc
      181 gtcgtacttg aactcgacct cctggccttc ggcccgttcg tcggtgatct ggatccgcag
      241 gccgcgggtc aggaaggcgg tttcacgaag ccgttcgagc agggtctgga aatcgaagtc
      301 gagttcctcg aaaatctcgt cgtccggcag gaaggtgatc tgggtgcccg tctctttggt
      361 cgcctctccc ttttcgagtt cggtgaccgg gcggccgcgc tcataacgct ggttccagac
      421 gaagccgtcg cgacagatcc ggagttcgag ccagctcgaa agcgcgttga ccaccgaaga
      481 gccgacgcca tggaggccgc ccgaaacctt gtacccgccg ccgtcgccga acttgccgcc
      541 ggcgtgcagg acggtcagca ccacttcggc cgcggggcga ccctccttct ccatgatgtc
      601 gaccggaatg ccgcggccgt ggtcggtgac cgtgcagctg ttgtcgggat ggaggttgac
      661 ctcgaccgac tcgttgtgcc cggccagggc ctcgtcgacc gagttgtcga cgatctcgta
      721 aaccaggtga tggagacccc ggggtccggt cgagccgatg tacatacccg ggcgcttgcg
      781 gaccgcctcg aggccctcga ggacggtgat gtccttggcc ccgtaggagg cgtcgccgct
      841 gctctttttc tcagccaatc agtgcctttc caaaaacttt tcggcggcca ccgccgtacc
      901 ggctacctgt ggagaaggtg cgcccggggg gaaccgcgag tcaggtctcc ataataccat
      961 tcccggtcgg ataaacccgc ctgccagagc caaaaaagcc ctgcaaaagc gatgtttttg
     1021 cacttatgtc agtgacttgt tgcacttcgg gctcatctgg ccgcccgaaa tcgcagtttt
     1081 tcgggtaccg gaccgtcgat ccgatccttg agccgggcca ggatccgggg cgccatcagc
     1141 tccagttcct gggcccagac ggcactcgaa cactcgactg tcacgacccc ttcgcgctcg
     1201 tcaatcacgt cggttacccc cgcgatccgg gggcccacga cctcgtccca ttgcgtctga
     1261 accgcagcca ggagggtcgg cggggtgacc tcgccccgga aatcacccag cacttccccc
     1321 agccgcttcg gggaggggct cctgccgcgt gcgtctctca cgaagtccct cccccggacc
     1381 cggcgccggg gtcctccccg ttgtcccgct cttcatctgc gccgaccagg tccctgatcg
     1441 cgacccggga gatctcctgc cgctccggga tcaactcgcc ctcggccgcg gtgatcacgc
     1501 tctggccacc cccgagcagg cgctcgacca gacggcctcg ccggtgggcg tcgagttcgc
     1561 tcatcacgtc gtcaagcaac atcagcggcg cggcctcgcc ggcatcgagc agcgccgccc
     1621 gctcggcgaa cagcagggcg tggaggccca gccgctgttg cccctgggat ccgaaacgcc
     1681 tgagctggcg gccgtcgagc tccagcctca cctcgtcgtg gtgcgggccc cagctgcttc
     1741 gcccgaggcg caggtcatgc tccagccgct cctcgagacc ggcttcgatc tcctccttgg
     1801 tggtggcgga tccgggccgg taactgatcc ccttcgggcc ttccaggccg aggtcatcag
     1861 ccgcctcgac ccagcgctcg gccagcgctt caaccgccgc ttcgcgaccc gaagcaaggt
     1921 tcgcgcccgc ttccgcgaac tgtgaattcc acgtggccag ttgagaagaa ttggcttcac
     1981 cggaggaaat gcgggcgatc agggcattcc tctgcgcgag gatccggccg aaagtggatc
     2041 gcaacccggc ccggcttggc caccgggcgg ccacgaaact gtcgaggtgc gcccgccggc
     2101 ccgccggggg gcctttgacc aactccagcc ggtccggcga gaagacggtc accttcggcc
     2161 ggtggaggac cgcttcggcc tgctcgatcc tggctccgtc catggtgaag cgattgccct
     2221 ccgagcggga agccgaggcc atgaagacgt ggtcgacccc accgccctgg acggtgaccc
     2281 ggcaccgggc aaaggctgaa ccgaagggga tcaggtcccg ccggtcggag gtcctgaacg
     2341 agcgcgaggt cagaccgaag tagatcgcct cgacgagggt ggtcttgccg gctccgttcg
     2401 gaccgatgat cacggtcagg ccggggtcgg gttcgaaa
//
LOCUS       DAOQMF010000242         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26964_length_2436_cov_4.405712, whole genome shotgun sequence.
ACCESSION   DAOQMF010000242 DAOQMF010000000
VERSION     DAOQMF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26964_length_2436_cov_4.405712"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /gene="gluQRS"
                     /locus_tag="PLV77_04985"
     CDS             <1..785
                     /gene="gluQRS"
                     /locus_tag="PLV77_04985"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNC15232.1"
                     /translation="GRLHLGNLRTALIAWRSARDRGARFLVRIEDIDTGRSRDEWIEV
                     QLEELRQIGVDWDGEPVRQSERHDLYREALSKLEADGLLYECFCTRAEIREAASAPHG
                     ELPEGAYPGTCRELTEAERAGRRAAGRPPALRVRAAGARVGFDDHLCGRVEKTLDDFV
                     VRRNDGDFAYNLAVTVDDADQGIGEVLRGADLLDTTPRQLWLYDRLGLAAPARFSHVP
                     LMLGEDGERLAKRHGAITLEERIEAGESPASVRRELFDSADR"
     gene            872..>2436
                     /locus_tag="PLV77_04990"
     CDS             872..>2436
                     /locus_tag="PLV77_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15233.1"
                     /translation="MKKFILGMTLSLFMLGAAQADAVPKYNASCMFNGCGGVSQEGQR
                     AIFTFDDMYPGPDEDFDIYERVGGQTRPLIDYPDGKKGHALLQFVSDDARRAIVETRS
                     PLTADDTDGNGEDFFAYQDGVPELISWDPADPSTRAGDIDLQLINATDDGKTLYFFRF
                     NKATPLCHELWKRTPTSFSRVTNDCDLLWMRGLSRDGSSVFYDDYSNGLWRVKDDARV
                     PVNDQPHPVNVHCTKYNYFGDATEDGEAWLFSSSYRLADADTDDLFDIYLRHRDGSYE
                     LITDTADGAEPEDCTSQNWLTRAIGLSPDMTRALFVTEFPISPDDHDQSLDVYLHTPQ
                     GNELVSTSPTDQSPEIRNPTSVHLQGGRPDSEFRVDGSDDLSVIAFDSEQQLVPEDND
                     SDVDVYARIGDETRLVSTGPNPDRNSRKAKLLGVSNDGTTISFSTPEPLLPVDTDGRM
                     DIYAYSTSGLSRLGDAAASASRKKRRVVLVSAESDAPRMKVLGKPSVSGRRALVRLRC
                     PKSEMTGPCRGTVR"
BASE COUNT          438 a          824 c          757 g          417 t
ORIGIN      
        1 ccgggcggct ccatctcggc aacctgagga cggccctgat cgcctggcgc tcggcccgag
       61 atcgcggggc gcggttcctg gtccggatcg aggacatcga caccggccgc tcccgggacg
      121 agtggatcga agtgcagctc gaggagctcc gccagatcgg agtcgactgg gacggcgagc
      181 cggtccgtca gagcgagcgc catgaccttt atcgcgaggc actttccaaa cttgaagccg
      241 acggcctgct ctacgagtgc ttctgcaccc gggccgagat ccgcgaagca gcgtcggccc
      301 ctcacggcga gttgccagaa ggtgcctacc ccggcacctg tagggaactc accgaggccg
      361 agagggccgg gcgccgggct gccggccggc ccccggcact ccgggtccgg gcggccggag
      421 ccagggtcgg cttcgatgat cacctctgcg gccgggtcga aaagaccctc gatgacttcg
      481 tggtgcgccg caatgacggt gacttcgctt acaacctcgc ggtcaccgtc gacgatgccg
      541 atcaggggat cggcgaggtg cttcgcggcg ccgacctgct cgacaccacc ccccgccagc
      601 tctggctcta cgaccgcctc ggccttgccg ccccggcccg tttcagccat gtccccctga
      661 tgctcggcga agacggagag cgcctcgcca agcgccacgg cgcgatcacc ctcgaggagc
      721 ggatcgaagc aggagagagc ccggcgtcag tccgcaggga gttgttcgat tcggctgatc
      781 ggtagcctca acgcaacatt cttgaacatg ccgtgttcaa ttttgtccat tgcaatcgac
      841 ggagccggag cgctccgaac aggggaaaat cttgaagaag ttcatcctgg ggatgaccct
      901 cagccttttc atgctcggcg ccgctcaggc tgatgccgtg cccaagtaca acgcgagctg
      961 catgttcaac ggttgcgggg gcgtctccca ggaaggccag cgtgcgatct tcaccttcga
     1021 tgacatgtac cccggccctg acgaggactt cgacatctac gagcgggttg gcggtcagac
     1081 ccggccgctg atcgactatc cggacggcaa gaagggtcat gccctgcttc agttcgtctc
     1141 cgacgacgcg cgtcgcgcga tcgtcgagac ccggtccccg ctcacggctg acgacaccga
     1201 cgggaacggc gaggacttct tcgcgtacca ggatggcgtc ccggagctga tctcctggga
     1261 tccggcagac ccctcgacca gggccggtga catcgacctg cagttgatca acgccaccga
     1321 tgacggcaag accctctatt tctttcgctt caacaaggcg accccgctct gccatgaact
     1381 ctggaagcgg accccgacga gcttctcgcg ggtgaccaat gactgcgatc tgctctggat
     1441 gcgaggcctt tcgcgcgacg gaagctcggt cttctacgac gactattcca acggcctgtg
     1501 gcgggtcaag gatgacgccc gggttccggt caacgaccag ccacatccgg tcaatgttca
     1561 ctgcacgaag tacaactact tcggcgacgc gaccgaggac ggagaggcct ggctgttctc
     1621 ctccagctac cggctggccg acgccgacac cgacgatctg ttcgacatct atctgcgtca
     1681 ccgggacggc agctacgagc tgatcaccga caccgctgat ggagctgagc ccgaggactg
     1741 caccagtcag aactggctga cccgggcgat cgggctttcc ccggacatga cccgggccct
     1801 gttcgtgacc gagttcccga tctcgccgga cgaccacgat cagtcgctcg atgtctatct
     1861 ccacacaccg cagggaaacg agctcgtttc gaccagcccg accgaccaaa gcccggagat
     1921 tcgcaacccg acctcggtcc atctccaggg gggtcggccc gactcggaat tccgggtcga
     1981 cggctccgat gacctctcgg tgatcgcctt cgattccgag cagcagctgg ttcccgagga
     2041 caacgattcc gatgtcgatg tctacgcacg cattggcgat gagacccggc tcgtctcgac
     2101 cggaccgaac ccagaccgga attcgcggaa ggcgaagctg ctcggcgtct cgaacgacgg
     2161 aacgaccatt tccttcagca ctcccgaacc gctgcttccg gtcgacaccg acggtcgcat
     2221 ggacatctac gcctactcga ccagcgggct ttcgcgcctt ggtgatgccg ccgcgtccgc
     2281 cagccgcaag aagcgacggg tggtactggt ctcggccgaa tcggacgctc cgcggatgaa
     2341 ggtactgggc aagccgtccg tttcgggtcg ccgggccctg gtcaggctgc gttgcccgaa
     2401 gtccgagatg accggtccct gccgggggac ggtgcg
//
LOCUS       DAOQMF010000243         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_26967_length_2436_cov_3.750945, whole genome shotgun sequence.
ACCESSION   DAOQMF010000243 DAOQMF010000000
VERSION     DAOQMF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26967_length_2436_cov_3.750945"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /gene="ppk1"
                     /locus_tag="PLV77_04995"
     CDS             <1..1315
                     /gene="ppk1"
                     /locus_tag="PLV77_04995"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNC15234.1"
                     /translation="RLEVADGMNPELKELLVELLRLQQNEVFEAGGLLDLTDLWDVVK
                     LGGFQELRDKPWAPVTQPRLQGEDGDPPDIFAAIRQRDVLVHHPYDSFSSSVERFIAQ
                     AVSDPDVLAIKQTVYRTSEDSPLVSALIKASERGKQVVCMLELKARFDEEANIEWAKR
                     LEEVGVHVVYGIPGLKTHIKALLVVRREGDQVRNYVHIGTGNYHSTTARLYTDLGLFT
                     ADPEIGADVAEMFNLLTGYSRPADYRRVLVSPHSMRSSILAEIEATVAAHKAGREAKI
                     LMKMNSLVDRTCIEALYRASQAGVEVEINVRGICCLRPGVPELSENIRVVSLVGRFLE
                     HSRVYAFQRGGDWNIFIGSADLMPRNLDARVELVTPVEDEIARDQILDVMERCFSDNS
                     NSWVLDSSGNWSRLSPEGGERRNAQEELMERHQTLAATALPQANG"
     gene            1379..1996
                     /locus_tag="PLV77_05000"
     CDS             1379..1996
                     /locus_tag="PLV77_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNC15235.1"
                     /translation="MRFSLLPRDNTFFDLFNEAGQNTLRAATKLNDMMAAWPDDNGLG
                     REILIIEQEGDRITHDIINRLNSTFVTPIDREDIYGLATKLDDVVDYIEEAADFLALY
                     QIEAPMDQAQDLTRILVGCCEQLSLGLENLPSFRDLDQYWIEIHRLENDGDRVSRDAV
                     ASLFSNGIDPMVVIRWKDIFAVLEKAIDATETSAHILEGIVIKNS"
     gene            2001..>2436
                     /locus_tag="PLV77_05005"
     CDS             2001..>2436
                     /locus_tag="PLV77_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNC15236.1"
                     /translation="MDLHLILVIVVVSALAFDFTNGFHDTANVVATSISTGAASPKAA
                     IAFAAILNFIGAFISIEVAATIASDVVDQSAIQGAGSLNVVFAGLVGAIAWNLITWYF
                     GLPSSSSHALIGGIVGAALADSGSQAIETAGLVGKVLVPAIVA"
BASE COUNT          450 a          791 c          760 g          435 t
ORIGIN      
        1 gcggctggaa gtcgccgacg ggatgaaccc ggagctcaag gagctgctgg tcgagctgct
       61 tcgtcttcag caaaacgaag tattcgaagc cggcggcctg ctcgacctga ccgacctctg
      121 ggacgtggtc aagctgggcg gcttccagga gctgcgcgac aagccctggg ctccggtcac
      181 ccagccccgg ctgcagggcg aggacggaga cccgcccgac atcttcgcgg cgatccgcca
      241 gcgcgacgtg ctggtccatc acccctacga ttccttctcg agttcggtcg agcggttcat
      301 cgcccaggcg gtgagcgatc cggacgtgct ggcaatcaag cagaccgttt accggaccag
      361 cgaggactcg ccgctggtct cggccctgat caaggcatcg gaaaggggca agcaggtcgt
      421 ctgcatgctg gaactgaagg cgcgcttcga cgaggaagcg aacatcgagt gggccaaacg
      481 ccttgaagaa gtcggggttc acgtggtcta cggcatcccc gggctgaaga ctcacatcaa
      541 ggccctgctg gtggtgcggc gggaaggcga ccaggtccgc aactacgtcc acatcggcac
      601 cggcaactat cactcgacca ccgcccggct ctacacggac ctcggcctgt tcaccgccga
      661 cccggagatc ggcgccgacg tggccgagat gttcaacctg ctgaccggtt actcgcgccc
      721 ggccgactac cgccgggtgc tggtctcgcc gcattcgatg cgttcctcga tcctggccga
      781 gatcgaagcc acggtcgccg cccacaaggc aggccgggag gcgaagatcc tgatgaagat
      841 gaactcgttg gtcgaccgga cctgcatcga ggcgctttac cgggcctcgc aggccggtgt
      901 ggaagtcgag atcaacgtgc gcgggatctg ctgcctcagg cccggggtgc cggagctttc
      961 ggagaacatc cgggtggtct ccctggtcgg ccggttcctc gagcattcac gggtctatgc
     1021 cttccagcgc ggaggggact ggaacatctt catcggctcg gccgacctga tgccgcgaaa
     1081 tctcgacgcg cgggtcgagc ttgtgacccc ggtcgaagac gagatcgccc gggatcagat
     1141 tctcgacgtg atggaacgct gcttctccga caactcgaac tcgtgggtgc tggacagttc
     1201 cggcaactgg tcccgcctca gccccgaggg cggcgagcgc cgcaacgccc aggaggaact
     1261 gatggaacgg caccagaccc tggccgcaac cgcgcttccg caggccaacg gatgaaaaga
     1321 ccatgaaaag gccgtgtaaa ggatttgcgg cccgctctgg taggttggcc cggctctgat
     1381 gcgtttctcg ctactgccca gggacaacac cttcttcgac ctcttcaacg aggccgggca
     1441 gaacaccctg cgcgcagcca cgaagctgaa cgacatgatg gccgcatggc ctgatgacaa
     1501 cggcctcggc cgcgagattc tgatcatcga gcaggagggt gaccggatca ctcacgacat
     1561 catcaaccgg ctcaattcga ccttcgtcac gccgattgac cgcgaggaca tttacggcct
     1621 cgcgaccaag ctcgacgacg tggtcgatta catcgaggaa gcggcggatt tcctggcgct
     1681 gtaccagatc gaagcgccga tggaccaggc ccaggacctg acccggattc tggtcggctg
     1741 ctgcgagcag ctttcgctcg gcctcgagaa cctgccgtca ttcagggatc tcgaccagta
     1801 ctggatcgag attcaccggc tcgaaaacga cggcgaccgg gtttcgcgtg acgccgtggc
     1861 atcgctgttt tcgaacggga tcgatccgat ggtcgtgatt cgctggaagg acatcttcgc
     1921 cgtcctcgag aaggcgatcg atgcaaccga aacctccgcc cacatccttg aaggcatcgt
     1981 gatcaagaac agctgacgac gtggacctcc atctgatcct cgtcatcgtt gtcgtctcgg
     2041 cactggcctt cgatttcacc aacggctttc acgacaccgc aaacgtcgtg gccacctcga
     2101 tctcaaccgg ggcggcttca cccaaggcgg cgatcgcctt cgccgcgatc ctcaacttca
     2161 tcggcgcctt catctcgatc gaggtggcgg cgacgatcgc ctcggacgtg gtcgaccagt
     2221 cggcgatcca gggtgcgggc tcgctcaacg tggtcttcgc cggtctggtc ggcgcgatcg
     2281 cctggaatct gatcacctgg tacttcggcc ttccttcctc cagttctcac gccctgatcg
     2341 gcggcatagt cggagcggcc ctcgccgact ccggcagcca ggcgatcgag accgccgggc
     2401 tggtcggcaa ggtgctggtg ccggccatcg ttgcgc
//
LOCUS       DAOQMF010000244         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27036_length_2433_cov_3.757359, whole genome shotgun sequence.
ACCESSION   DAOQMF010000244 DAOQMF010000000
VERSION     DAOQMF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27036_length_2433_cov_3.757359"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PLV77_05010"
     CDS             complement(<1..978)
                     /locus_tag="PLV77_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15237.1"
                     /translation="MSTFATGNGGWTSSTDYGGVCVPAVTCPELSGSYQSTGGAAGGS
                     DGYIRTDSGELTVASLLSTSTHTWRSPAFTYNGVGGAQPEGLHFSVGVNPAVAQLLNL
                     GVDIGISAKFTGVSGGGDQIVIDDVTPGTASGWKTLNGQIGPDALEVGKRYRVELSVS
                     VGGLAAVLPSGSIGFDDVSLRATGPSGNGNGNGNGNGNGNGNGTGNGNGNGNGGSTVL
                     PPPKVIPAGKAFLYRNKLYIRVKCPRAFRPRCRINLVALTRKRHGRKLTPVRRANVRR
                     GHSGRKVLGVRRPFRVKVRKMAKAGKRIVVRQKIRSKRGRKHAIRYQRLR"
     gene            971..1114
                     /locus_tag="PLV77_05015"
     CDS             971..1114
                     /locus_tag="PLV77_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15238.1"
                     /translation="MLTFPLGYVTVAAFAPAGISQAISIDNAAIAAAGKSLLRIMLIS
                     SLH"
     gene            complement(1256..1777)
                     /locus_tag="PLV77_05020"
     CDS             complement(1256..1777)
                     /locus_tag="PLV77_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNC15239.1"
                     /translation="MESLKGRLLVAAPSLFDFFRRTVVLVVEHTDQGAFGVILNRRAD
                     TEVAELVPDLGPLVEQGAPLWVGGPVGPDSIVVLGDFNHPDQSAGVVEGSIGVVDPDG
                     DADFSRARVFVGHAGWGPGQLEGEVERDSWIVSDFDPDDVFSEGDLWAEVVRRPGGEL
                     AILATMPDDPTLN"
     gene            1884..>2433
                     /gene="rpsD"
                     /locus_tag="PLV77_05025"
     CDS             1884..>2433
                     /gene="rpsD"
                     /locus_tag="PLV77_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNC15240.1"
                     /translation="MGRYTGPKEKLSRREGVDLGLKGERALGGKSAMERRPYPPGQHG
                     RGRRRASEFSVRMRAKQRAKAMYGLREKQFAKLFDRANREEGMAGENLMRHLESRLDN
                     VVYRLGLASTRAQARQFVVHQHIQVNGKRLDRPSYEVSAGDEIAVKPGTAIEAMARQS
                     TESAGPTPAWLLADHDGLKGTVN"
BASE COUNT          422 a          829 c          768 g          414 t
ORIGIN      
        1 gcgaaggcgc tggtatcgaa tcgcgtgctt gcggcctcgt ttggacctga tcttctgtcg
       61 cacgacgatc cgtttcccgg ccttggccat cttccggacc ttgacccgga acgggcgcct
      121 gacgccgagg accttgcgtc cggaatggcc ccggcggacg ttcgccctgc ggaccggggt
      181 caacttccgt ccgtgccgct tcctggtcag ggcgaccagg ttgatccggc agcgcggccg
      241 gaaagcccgc gggcacttga cccggatgta gagcttgttc cggtagagga aggccttccc
      301 ggccggaatc accttcggcg ggggcagcac ggtgctgcca ccgtttccgt tgccgttgcc
      361 attgccggtg ccgttgccgt tcccgtttcc attcccgttg ccgttgccat ttccgttgcc
      421 ggagggaccg gtggctcgca gcgagacatc gtcgaacccg atcgagccag acggcaggac
      481 cgcagccagg ccgccgaccg aaaccgacaa ttcgacccgg tatcgcttgc cgacctcgag
      541 cgcgtcgggc ccgatctggc cgttcagcgt cttccagccg gaggcggtgc cgggggtgac
      601 atcgtcaatc acaatctgat ctccaccgcc ggaaacgccg gtgaatttgg cgcttatccc
      661 gatgtcgaca cccaggttca gcaactgcgc caccgcggga ttgaccccga ccgagaagtg
      721 cagcccttcg ggctgtgcgc cgccgacccc gttataggtg aaggcggggc tccgccaggt
      781 gtgggtggag gttgaaagca gggaggcgac ggtaagttcg ccggaatcgg tccggatgta
      841 cccgtcgctt ccgccggctg cgccaccggt tgactggtag ctgccggaaa gctcgggaca
      901 ggtcactgcc ggcacacata ctccgccgta gtcggtgctc gaggtccagc caccgttgcc
      961 ggtggcgaaa gtgctcacgt tcccgctcgg atatgtcacg gtggccgcct tcgccccggc
     1021 cgggatcagc caggcgatca gtatcgacaa cgccgcgatt gccgcagcag gcaagagtct
     1081 gctccgcata atgctcatct cttctctcca ttgagttcgg gttggcgatc cgtgccgttc
     1141 ggcccagggg gatcgcttac cgttcaatcg ggtgacggcg ttaccgccac cgccaacgga
     1201 accactattc ccgaagccac cgcttcgcaa acagggtgtt ccggtcctct tgctttcagt
     1261 tcagagtcgg gtcatccggc atcgtggcga ggatcgccag ttcgcccccc ggccggcgaa
     1321 cgacctcggc ccagagatcg ccttccgaga agacatcatc gggatcgaag tcggaaacga
     1381 tccaggaatc gcgctcgact tcaccttcga gctgtcccgg cccccagccg gcgtgtccga
     1441 cgaagacacg ggcccgggag aagtcggcat caccgtccgg gtcaaccacc ccgatgctgc
     1501 cctcgaccac cccggccgac tggtccggat ggttgaaatc cccgagcacg acgatcgagt
     1561 cgggaccgac cgggccaccg acccagagcg gagccccctg ctcgaccagc ggcccgagat
     1621 cgggaaccag ttcggccacc tcggtgtcgg cgcggcggtt gagaatcacc ccgaaggccc
     1681 cctgatcggt gtgttcgacg accagcacaa cggtgcgccg aaagaaatcg aaaaggctcg
     1741 gtgcggctac cagcaagcgt cccttgagcg actccatgta ccgactatag gacgctgttc
     1801 ggcgtcgagt ggacgcggac cggccaaggc gctgccgcac aaagcgggaa ttcgcccgat
     1861 ccggtaggct ccttcgccgc actatgggca gatacacagg accaaaagaa aagctttcgc
     1921 gccgtgaggg cgtagatctc ggactcaagg gtgaacgggc cctcggcggc aagagcgcca
     1981 tggagcgtcg cccctacccg cccggccagc atggccgcgg ccggcgccgt gcttcggagt
     2041 tctcggtccg tatgcgtgcc aagcagcgcg ccaaggcgat gtatggcctg cgcgagaagc
     2101 agttcgccaa gctcttcgac cgcgccaacc gtgaagaagg catggccggc gagaacctga
     2161 tgcgtcacct cgagagccgt cttgacaacg tcgtttaccg cctcggtctc gcttcgaccc
     2221 gcgcccaggc ccgccagttc gtcgtgcatc agcacatcca ggtcaacggc aagcgcctcg
     2281 accgtccctc gtacgaggtt tcggccggtg acgagattgc cgtcaagccc ggtaccgcga
     2341 tcgaggccat ggcccgtcag tccaccgagt cggctggccc gaccccggcc tggctcctgg
     2401 ccgaccacga cggtctgaag ggcacggtca acc
//
LOCUS       DAOQMF010000245         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27083_length_2430_cov_3.950316, whole genome shotgun sequence.
ACCESSION   DAOQMF010000245 DAOQMF010000000
VERSION     DAOQMF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27083_length_2430_cov_3.950316"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PLV77_05030"
     CDS             <1..1251
                     /locus_tag="PLV77_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNC15241.1"
                     /translation="NGNFFDRIWVFVTHLDQVDLPTVAIGLTSLAIILICKYRHPSAP
                     GSLIAVGLGIAAVSLLDLGSDGVAIVGEIKSGLPSVALPNVGLQDLGDLVAGAVGLML
                     VGFAESLAAAKAYPDEDEELDADRELIGTGAANVGAGLFGGFVVTGSFSKTSINSESG
                     ARSQMAGIVVALMVVITMLFLTGLFEDLPEAALAAVVIAALIHAVDVVGLSELARIRD
                     RGNRLNPANRPDFIAAVAALIGVTAFDILPGLFIGIVVSLVLLLYRSSRPHVTRLGEL
                     RAQPGHWGDLQRHANAVEVPQIVVLRLEAAIYFANSDEIRQAVIRSLAADTRAVVLDV
                     ETVPYIDVTAAYMLKALREELDQRQVRLVIARDVGQVEDMMRSTGLEVLLTDSYVSID
                     QAVDSVTAGIRSTSGDRPSGAATT"
     gene            complement(1468..>2430)
                     /locus_tag="PLV77_05035"
     CDS             complement(1468..>2430)
                     /locus_tag="PLV77_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC15242.1"
                     /translation="DFGTLLGRLERCSVIGLLDVDDMVLLNPPGSKVISSGDRLIAVA
                     EDDTVLELFEESVSGPDFEQILAADERSPSPERVVILGWNKGAQPVITHFDAFLAPGS
                     RIEVVVDPGLGRAPEVDSHQRLANTDVNFRHGSTTDRSTLEELELGQADHVMVLACSD
                     DFDAQRADARTLVTLLHLREINDGLEPGFSIVSEMVDERSRRLAEVAEVDDVIVSENV
                     ISLLFAQISQNHHLADVFTQLFSPEGSEIYLRPAGTYVALNEEVSFATMVEAARRRGE
                     CAIGYRSASQSDDPDSGFGMTINPPKSRVFKVTQGDSLVVLAEE"
BASE COUNT          393 a          801 c          796 g          440 t
ORIGIN      
        1 aacgggaact tcttcgaccg gatctgggtc ttcgtgaccc acctcgacca ggtggacctg
       61 ccgaccgtgg cgatcgggct cacctcgctg gcgatcatcc tcatctgcaa gtaccggcac
      121 ccgtcggcgc ccggctccct gatcgcggtc gggctgggga tcgccgcggt cagcctgctt
      181 gaccttggct ccgacggcgt cgccatcgtc ggcgagatca agtccggcct gccttcggtt
      241 gccctgccga acgttggcct tcaggatctc ggtgacctgg ttgccggcgc ggtcgggctg
      301 atgctggtcg gctttgccga aagcctcgcc gcggccaagg cctatcccga cgaggacgag
      361 gagctcgatg ccgaccggga gctgatcggc accggcgcgg ccaacgtggg agccggcctc
      421 ttcggtggct tcgtggtcac ggggagtttc tcgaagacct cgatcaactc cgagtccggg
      481 gctcgcagcc agatggcggg catcgtcgtc gccctgatgg tcgtgatcac gatgctcttc
      541 ctcaccggtc tcttcgagga cctcccggaa gcggccctcg ccgccgtggt gatcgccgcc
      601 ctgatccacg ccgtggacgt ggtcggtctg agtgagctcg cccggatcag ggaccgcggc
      661 aaccggctca accccgccaa ccggcccgac ttcatcgccg cggtcgccgc gctgatcgga
      721 gtcacggcct tcgacatcct gcccggactc ttcatcggca tcgtggtttc gctggtgctg
      781 ctgctttacc gctcctcgcg gccgcatgtg accaggctcg gtgagctcag ggcgcagccc
      841 ggacactggg gtgacctgca gcggcacgca aacgcggtcg aggtcccgca gatcgtggtg
      901 ctgagactcg aagcggcgat ctatttcgcc aattcagacg agattcgcca ggcggtgatt
      961 cgaagccttg cggccgacac ccgggcggtg gttctggacg tggagacggt gccctacatc
     1021 gacgtgaccg ccgcctacat gttgaaggcg ctgcgggagg aactcgatca aaggcaggtc
     1081 cggctcgtca tcgcccgcga cgtcgggcag gtcgaggaca tgatgagatc gaccggcctc
     1141 gaggtgctgc tgaccgactc ctatgtctcg atcgaccagg cggttgattc ggtgaccgcg
     1201 ggcattcgct caacctccgg cgacaggccg tcaggcgccg ccacaacctg accggttcgc
     1261 cgcggtgccg aacacctttc ggaaagtttt ttgtccggtt cgaatttcac cgcaaccctt
     1321 ggccacctgc ccggttatag gggcagtccg cgtgccaggg agggatgcac ggcagggact
     1381 gacagaagca gggggaggtg gcgaggcccc aggcctcgcc gccttgcatt caacgacccg
     1441 gccggccctc tgctccgaac agaagtctca ttcctctgcg aggacgacga ggctgtcgcc
     1501 ttgtgtgacc ttgaagaccc gggacttggg cgggttgatc gtcatgccga aacccgagtc
     1561 cgggtcatcg gactgtgaag ccgagcgata cccgatcgcg cattccccgc ggcggcgggc
     1621 cgcctcgacc atggtcgcga aactgacctc ttcgttcagc gctacgtagg tcccggccgg
     1681 ccgcaggtag atctcgctcc cctccggaga gaagagctgg gtgaagacat cggccagatg
     1741 gtggttctgc gagatctgtg cgaagagcag gctgatcacg ttctcgctga cgatcacgtc
     1801 atcgacctcg gccacctcgg ccagccggcg gcttcgctcg tccaccatct cgctgacgat
     1861 cgagaagcca ggctcaagac cgtcgttgat ctccctcagg tggagcaggg tgaccagggt
     1921 gcgggcgtcg gcgcgctggg catcgaagtc atccgagcag gccagcacca tcacgtggtc
     1981 ggcctgccca agctcgagtt cctccagggt ggagcggtcg gtggtcgaac catgtcggaa
     2041 attcacgtcg gtattggcga ggcgctggtg gctgtcgact tccggtgccc ggcccaggcc
     2101 gggatcgaca accacctcga tccgtgatcc gggcgcgagg aaggcgtcga agtgggtgat
     2161 gaccggctgc gcccccttgt tccagccgag gatcacgacc ctttccggcg aagggctccg
     2221 ttcgtcggcc gccaggatct gctcgaagtc gggaccggag accgattcct cgaaaagctc
     2281 gagcaccgtg tcgtcttcgg ccacggcgat cagcctgtct ccggacgaga tcaccttcga
     2341 gccgggcggg ttcagcagaa ccatgtcatc cacgtccagc aggccgatca ccgaacacct
     2401 ttccagccgg ccgagcaggg tgccgaagtc
//
LOCUS       DAOQMF010000246         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27088_length_2430_cov_3.530947, whole genome shotgun sequence.
ACCESSION   DAOQMF010000246 DAOQMF010000000
VERSION     DAOQMF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27088_length_2430_cov_3.530947"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLV77_05040"
     CDS             complement(<1..184)
                     /locus_tag="PLV77_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC15243.1"
                     /translation="MSDATRSSQGGSAVLEIQGVTKAFGERQVLGGIDLTLREHEAIA
                     LIGASGSGKSTLLRCID"
     gene            complement(181..852)
                     /locus_tag="PLV77_05045"
     CDS             complement(181..852)
                     /locus_tag="PLV77_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNC15244.1"
                     /translation="MFFSAESFRDSFPDILKAFWIDIKLFMVVEAIVLVLGLLIAVVR
                     TSKASALFPIRMLAAVYCDFFRGIPVILLVYLVGFGIPALGLAGVPTDPLILAGIALS
                     LAYSSYVAEVYRAGIASIHPSQNDAGLATGLTRIQTLRFVIVPQAVRRVRPPLLNDFI
                     SLQKDVALVSVLGIAEAFRTAQIITLSTFNYTPLLAAALLYLAVTFPMARILDHITAR
                     EQINS"
     gene            complement(1039..1920)
                     /locus_tag="PLV77_05050"
     CDS             complement(1039..1920)
                     /locus_tag="PLV77_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC15245.1"
                     /translation="MSRKRLVLFLFAIFALAVFVGCGSSDDDSSSENSGTDSGSTVAA
                     SDCTPANLSTFKDGVLTIGTDSPAYPPYFEDDDPTNGKGFESALGYAIAGELGFNDSQ
                     VEWATVPFNASYAPGPKDFDFDLNQISITPAREKAVTFSEPYYTANQAVLVKKGSDLD
                     GITSLDQLKDASIGVQIGTTSYDAVVEQIDPSSDPKTFDNSNDVVTAFKQDQVDAIVV
                     DLPTAIYLRDAELSDTVVAGQFEQEGGDQWGALMAKDSDLKGCVDQAINTLKEDGTLE
                     EITNQWMADSAKAPLLN"
     gene            1993..2412
                     /locus_tag="PLV77_05055"
     CDS             1993..2412
                     /locus_tag="PLV77_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC15246.1"
                     /translation="MSTEPKSQAVRDEEEGRPCVEMSEGVARYLMALFDLEQEGGPPT
                     QARLARELDVSQPSALQMVRRIRAMGLINEGDLGLTPAGTSSALVLTSRRTAATDLTS
                     EVLGLDPAEARVEAGHLASSATPAFGRKLVAWRKSRT"
BASE COUNT          451 a          737 c          831 g          411 t
ORIGIN      
        1 ggtcgatgca gcgcagcagg gttgacttcc cggagccgga agcgccgatc agggcgatcg
       61 cttcatgctc ccgcagggtg aggtcgatcc cgccaagtac ctgacgctcg ccgaaggcct
      121 tggtcacacc ctggatttcc aggaccgctg aacctccctg gctggaacgc gtggcgtcgc
      181 tcacgagttg atttgctccc gggcggtgat gtgatcaagg atccgcgcca tcgggaaggt
      241 aaccgccagg tagagcaggg cggcggccag cagcggggtg taattgaagg tcgaaagcgt
      301 gatgatctgg gcggtcctga acgcctcggc aattccgagc acggaaacca gtgcaacgtc
      361 cttctggagc gagatgaagt cgttgaggag cggcggccgc acccgacgca ccgcctgggg
      421 cacgatcacg aaacgcagcg tctggatccg ggtcagcccg gtggccagac cggcgtcatt
      481 ctggctgggg tggatcgagg cgatgccggc ccggtagacc tccgccacat atgagctgta
      541 ggcgagcgaa agggcgatgc cggcgaggat caggggatcg gtaggcaccc cggccagccc
      601 gagggcgggg atgccgaagc cgaccaggta aacgagcagg atgaccggta tcccgcggaa
      661 gaagtcacag tagaccgccg ccagcatccg gatcgggaag agggccgatg ccttgctggt
      721 cctgaccacc gcaatcagca atccgaggac caggacgatg gcctcgacga ccatgaacag
      781 cttgatgtcg atccagaagg ctttgaggat gtccggaaac gagtccctga aggactcggc
      841 cgagaagaac atctcccgca cgtccggcca gccgctgctg gtcaggatga cggcggcaag
      901 gccaccgaga accaggaccg atgaaagaac ggcgatcgcc tggccgcgcc gcgccctggc
      961 ctttcgggcc ttgtcgcggg cggcccggcg atcgccggta ccgctttgaa ctggagcttc
     1021 cgtggtcaga cccggctctt agttgagcag cggcgccttg gccgaatcgg ccatccactg
     1081 gttggtgatc tcttccagcg tgccgtcttc cttcagcgtg ttgatcgcct ggtccacgca
     1141 acccttgagg tcggagtcct tggccatcag cgcgccccac tggtcgccac cctcctgctc
     1201 gaactgaccg gcaacgacgg tgtcggagag ctcggcgtca cggaggtaga tcgcggtcgg
     1261 aaggtcgacc acgatcgcgt cgacctgatc ctgcttgaag gccgtaacga cgtcattcga
     1321 gttgtcgaag gtcttcgggt cggatgaggg atcgatctgc tcgaccacgg cgtcgtagct
     1381 ggtggtaccg atctggaccc cgatcgaagc gtccttgagc tggtcgaggc tggtgatccc
     1441 gtcgagatca gagcccttct tgaccagcac cgcctggttg gcggtgtagt acggctccga
     1501 gaaggtgacc gccttctcgc gggcgggagt gatcgagatc tggttcaggt cgaagtcaaa
     1561 gtccttcggt ccgggcgcgt aggaagcgtt gaacggcacg gtcgcccatt cgacctggga
     1621 gtcgttgaat ccgagctcgc cggcgatcgc gtaaccgagg gcgctctcga agcccttgcc
     1681 gttggtcggg tcgtcgtcct cgaagtacgg cgggtaggcc ggggagtcgg tgccgatggt
     1741 cagcacaccg tccttgaagg tgctgaggtt cgccggagtg cagtccgaag cggcgacggt
     1801 gctgccactg tcggtgccgg aattttcgct ggacgagtca tcgtcactgg agccgcagcc
     1861 gacgaaaacg gccagggcga agatggcaaa caggaaaagg acaagacgtt tacgactcac
     1921 gagtattcct ccgcaggttg agcgactgac gtggggagcc tagagcatgt tgagggtaga
     1981 atcgctcgcg gtttgagcac agagccgaaa agtcaggccg tacgcgacga ggaggaaggc
     2041 cgtccctgcg ttgagatgag cgaaggcgtg gcccgttacc tgatggcgct gttcgacctc
     2101 gagcaggaag gcggcccccc gacccaggcc aggctggccc gggaacttga cgtttcccag
     2161 ccctctgcgc ttcagatggt ccgccggatc cgggcgatgg gcctgatcaa cgagggggac
     2221 ctcggactca cgccggccgg caccagctcc gctctggtgc tcacttccag gcggaccgcc
     2281 gcaaccgacc tgaccagcga ggtgctggga ctcgacccgg ccgaagcgcg ggttgaagcc
     2341 ggacatctcg cctccagcgc caccccggcc ttcggccgca agctggtcgc ctggcgcaag
     2401 tcccggacct gagcccgttc agttgacggt
//
LOCUS       DAOQMF010000247         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27111_length_2429_cov_4.415754, whole genome shotgun sequence.
ACCESSION   DAOQMF010000247 DAOQMF010000000
VERSION     DAOQMF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27111_length_2429_cov_4.415754"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="PLV77_05060"
     CDS             <1..1626
                     /locus_tag="PLV77_05060"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HNC15247.1"
                     /translation="VTSTQDVEFNWFGDPYFLLKHGYTFVGVTAQNVGVASLKAFDSD
                     RYGTLTVNGNDTVPTGTGLDADALSYDVYSSVVKALKGSRNGVDPLGGITPSMVIASG
                     ESQSCGRLVTHYNRIEPIHEVVDAYLLTVCTSALRGDRGEKAIRIITETENRVPRTVA
                     SDPDTGSIRHWEVAAASHLPRVAFDNLNPVFSRDFATVTANCVKFPLSRIKWPYTQNA
                     AISDLVGWVGDDAAPPIAPRGEYQASPPDMANQLERDENGIALGAIRYPDVTVPTATN
                     DGINSAAPGGSIFSAFCGLFGSSVPFSPELLHSLYTDQADYLAQYSEAADALADSGFI
                     LPEDADRLKGDSREYARLRPSLPSVIGGTSNKGGFQLVWVGTEAPDTTFEVQRASTKK
                     PNAWAKVKTKSVSDSTATIADAAQGTAYFRVNSATVLPGTNISEPETVVTPFSEASVA
                     VKIDRTGPAKPKIVLKGHKVKGQFRGPVRVKVIGKPDGKLPDGTAGVGLNPKSVPKTR
                     KVTRKGKTVIKIRTRDKLGNQSPVAKVVIRIRR"
     gene            complement(1634..2401)
                     /locus_tag="PLV77_05065"
     CDS             complement(1634..2401)
                     /locus_tag="PLV77_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC15248.1"
                     /translation="MSPSMVRLVSLTRREVSRFLKIPRQTVGAPLLETFLYISIFGAA
                     LGSRVGDLHGYEYIVFVVPGLIMMAFAINAFTNNSSSILQQKFQGAIDDQLSSPASPG
                     QLLLAFTLGGFLRGLLISVVTFLVASLLIDLPVKHFYILIPALFLVGWFFASLGVLVG
                     VRAEQFDDVSFAQTFILQPLIFLGGVFYSASLLPEPYETLTHFNPVYYMISLVRYGFL
                     GVADISVWLSLALLTAATAALTFANYRMFKSGYKLRR"
BASE COUNT          490 a          776 c          795 g          368 t
ORIGIN      
        1 gtcacctcga cccaggacgt cgagttcaac tggttcggtg acccgtactt cctgctcaag
       61 cacggttaca ccttcgtcgg ggtgaccgcc cagaacgtcg gtgtcgcctc cctgaaggcc
      121 ttcgacagcg accgttacgg gacgctgacg gtcaacggca acgacaccgt gccgaccggt
      181 accggcctgg atgccgacgc cctctcatat gacgtctact cctcggtcgt caaggcactg
      241 aagggcagcc gcaacggcgt cgatcccctg ggcgggatca cgccctcgat ggtgatcgcc
      301 tcgggtgaat cacagtcctg cggtcgtctg gtcacccatt acaaccggat cgagccgatt
      361 catgaagtcg tggatgccta tctgctgacg gtctgcacct ccgcgctccg cggcgaccgc
      421 ggggagaagg cgatccggat catcaccgag accgagaacc gggttccgcg aaccgtcgcg
      481 tccgatccgg acaccggttc gattcgccac tgggaggtcg cggcggcgtc ccacctgccc
      541 cgggtcgcct tcgacaatct gaacccggtg ttctcgcgcg acttcgccac cgtgacggcg
      601 aactgcgtca agttcccgct gtcgcgaatc aagtggccct acacccagaa cgccgcgatc
      661 agcgacctgg tcggctgggt gggtgacgac gccgcaccgc cgatcgcgcc acgcggcgag
      721 taccaggcga gtcccccgga catggccaac cagctggaac gcgacgagaa cgggatcgcg
      781 ctgggcgcaa tccgctaccc cgacgtgacg gtgcccaccg cgaccaatga cggcatcaac
      841 tcggcggctc ccggaggctc aatcttctct gccttctgcg ggctgttcgg ttcatcggtc
      901 ccgttctcgc ccgagctgct tcattcgctt tacaccgatc aggccgacta cctggcgcag
      961 tactccgagg cggccgacgc cctggccgat tccggcttca tcctgcccga ggatgccgac
     1021 cggttgaagg gggactcacg tgaatacgcg cggctccggc cgtcgctgcc gagcgtgatc
     1081 ggcggaacat cgaacaaggg cggcttccag ctcgtctggg tcgggaccga agcccccgac
     1141 acgaccttcg aggttcagcg ggccagcacc aaaaagccca acgcctgggc aaaggtcaag
     1201 acgaagtcgg tgagcgacag caccgcgacc attgcggatg ccgcccaggg aaccgcctac
     1261 ttccgggtga acagcgccac cgtgctgccc ggcaccaaca tctccgagcc ggagaccgtc
     1321 gtgaccccgt tctcggaagc cagcgtcgcg gtcaagatcg accggaccgg accggcgaag
     1381 ccgaagatcg tgctcaaggg tcacaaggtc aaaggccagt tcaggggccc ggtccgggtc
     1441 aaggtgatcg gcaagccgga cggcaagctg ccggacggga ccgccggcgt cgggctcaac
     1501 ccgaaatccg tgccgaagac ccgcaaggtc acccgcaagg gcaagacggt gatcaagatc
     1561 aggacccgcg acaagctcgg caaccagtcc ccggtcgcca aggtggtcat caggatcagg
     1621 cgctgatccg tcgttatctc cggagcttgt agccggactt gaacatccgg tagttggcga
     1681 aggtgagtgc ggcggtggcc gcggtgagca gggcaagtga aagccagacg ctgatgtcgg
     1741 cgaccccgag aaacccgtaa cggaccaggg agatcatgta gtagaccggg ttgaaatggg
     1801 tcagggtttc gtagggctcg ggcaggagcg aggccgagta gaacacgccg ccgaggaaga
     1861 tcagcggctg gaggatgaag gtctgggcaa acgagacgtc gtcgaactgt tccgccctga
     1921 ccccgaccag cacaccgagg ctggcgaaga accagccgac cagaaacagg gcgggaatga
     1981 ggatgtagaa gtgcttgacc ggcaggtcga tcagcaggct ggcgaccagg aacgtgacga
     2041 ccgagatcaa gaggccgcgc aggaagcccc cgagcgtgaa ggccaggagc aactggccgg
     2101 gcgaggccgg ggaggagagc tggtcatcga ttgccccctg gaacttctgc tgaaggatcg
     2161 atgacgagtt gttggtgaag gcgttgatcg cgaaggccat catgatcagg ccggggacca
     2221 cgaagacgat gtattcgtag ccgtgcaggt cacccacccg ggaaccgagc gcggcaccga
     2281 agatcgatat gtaaaggaag gtctcgagca gcggtgcgcc gaccgtctgg cgggggatct
     2341 tgagaaagcg gctgacctcg cgcctggtca gggagaccag ccgcaccatc gacgggctca
     2401 ctggccgtcc tccgcttcga ctccgacat
//
LOCUS       DAOQMF010000248         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27135_length_2428_cov_3.686894, whole genome shotgun sequence.
ACCESSION   DAOQMF010000248 DAOQMF010000000
VERSION     DAOQMF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27135_length_2428_cov_3.686894"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..874
                     /locus_tag="PLV77_05070"
     CDS             182..874
                     /locus_tag="PLV77_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNC15249.1"
                     /translation="MDSESQALPTLRQQGDVYLLDLGDGENRFNPDRNSVIEELLAEV
                     EAAPAPRALVTVASGKVWSNGLDLEWFQAHPDRVQEALDQTERLFAAFLGAGIPTVAA
                     IQGHCFAGGAMLALAHDYLIMREDRGYLCLPEADLGLPFTPGMNSLLLDRLPRRTAHE
                     VMVTGRRYGGTDAAAAGIVDQVASEDLLVPAAIERAAGQAPKNPETLRAIKRRIYERT
                     IELLETSHPLGS"
     gene            914..1123
                     /locus_tag="PLV77_05075"
     CDS             914..1123
                     /locus_tag="PLV77_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15250.1"
                     /translation="MAEEPVSKNKDGSRIGSDEYVTLALCIIVPIIGLLFALYYRQQG
                     EKWAGRAMVVGVVALVVWLCLVFLI"
     gene            1120..1698
                     /locus_tag="PLV77_05080"
     CDS             1120..1698
                     /locus_tag="PLV77_05080"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC15251.1"
                     /translation="MSRDFRELPPDLPVPVDDGAADHLEGMAFPELSLRSTSGGEVSL
                     PSAFGERAVLFTYPRTGTPGEPLLPGWDDIPGARGCTPQSCAYRENLAGFRSAGLAVF
                     GLSAQTAEAHAEFHARESIPYELISDPGLKLAETLRLPTFAISSLTLYRRLTMIIEAG
                     HIAKVFYPVFPPDQDATTVLAWLAGDDLAETS"
     gene            1731..>2428
                     /locus_tag="PLV77_05085"
     CDS             1731..>2428
                     /locus_tag="PLV77_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15252.1"
                     /translation="MSTPLRLLLYGLSVLAVFAVSFVAGKALIPEDTVSDWTRQADAS
                     SAGHESMAGDHDEAHVGGLSVEQDGYTISPVSSPSVAGQPGELSFRILDPEGKPLTGY
                     SSNHEKQLHLITVRLDGDRFRHVHPRLDLASGTWSIPWSWPAGGSYRVFADFVPQGSS
                     PDQALTLSRSVEVGGEFMPEPKPLSRTDSTGGFKATLRGELEAGSSSELTAHLTRGGR
                     PVTDLEPYLGAFGHL"
BASE COUNT          404 a          823 c          787 g          414 t
ORIGIN      
        1 gccgcggccg gaagtcagca tcaccaccga ggcgatgatg ccgatcgggc tgatcgccac
       61 gccgacggcc aggggaagtg tctgtccaac cgcctctccc acggccacag gataacccgc
      121 ccgatcggag ctcgcggctt tcgcctgtag gctcggcgga aaccagcaag aagggagcgc
      181 gatggattca gagagtcagg ctttgccgac cctgaggcaa cagggcgacg tttatctgct
      241 cgatctcggc gacggagaga atcggttcaa cccggatcgg aactccgtga tcgaggagct
      301 gctcgcggag gtcgaagcgg cacctgcgcc gcgggcgctg gtcaccgtgg cgagtggaaa
      361 ggtctggtcc aacggcctcg atctcgagtg gttccaggcg cacccggatc gggtccagga
      421 agccctcgat cagaccgaga gactgttcgc cgccttcctc ggtgccggga tcccgaccgt
      481 ggcagcgatc cagggccact gcttcgcggg cggggcgatg cttgctctcg cccacgacta
      541 cctgatcatg cgggaagacc gggggtatct atgcctgccc gaagccgacc ttggcctgcc
      601 tttcactccg ggcatgaact cgctgctgct cgaccgcctt ccccgccgga ccgcgcacga
      661 ggtgatggtc acggggaggc ggtacggcgg gaccgacgct gccgctgccg gaatcgtcga
      721 ccaggtggct tccgaggatc tgctggtccc ggcggcgatc gaaagggcgg ccggccaggc
      781 accgaagaac ccggaaaccc tcagggcgat caaacgccgg atctacgagc gaacgatcga
      841 gctgctcgaa acttcgcatc ccctcgggtc ctgaccggga cgcccgcgcg atctgcccgg
      901 gtagggttcg cccatggccg aagaaccggt aagcaagaac aaggacggca gcaggatcgg
      961 ctccgatgaa tacgtcacgc tggcgctctg catcatcgtg ccgatcatcg ggttgctctt
     1021 cgccctctac taccgccagc agggcgagaa atgggccggc cgagcgatgg tcgtcggcgt
     1081 cgtcgccctg gtcgtctggc tctgcctggt cttcctgatt tgagccgcga cttcagagag
     1141 ctcccgcccg acctgccggt tcccgtcgat gacggggccg cggaccacct cgaggggatg
     1201 gcgttcccgg agctcagcct gaggtccacg agcggcgggg aagtgagcct gccctcggcc
     1261 ttcggcgagc gggccgttct cttcacctac ccgcggaccg gcaccccggg ggagccgctt
     1321 ctgcccggct gggatgacat tcccggcgcc aggggttgta cgcctcagtc ctgtgcctac
     1381 cgtgaaaacc tggccggttt ccggtcggcc gggctggccg tcttcgggtt gagtgcccag
     1441 accgccgaag cccatgccga gttccacgcg cgggaatcga ttccctacga gctgatcagt
     1501 gatcccggac tgaaactggc cgagacgctg aggctgccga cctttgccat ttccagcctc
     1561 accctgtacc ggcggctgac catgatcatc gaagcgggac acatcgcaaa ggtcttttat
     1621 cccgtcttcc cgccggatca ggacgccacc acggtgctcg cctggctggc cggggacgac
     1681 ctggcggaga cctcttgaca tataccccat aggggtatag ggtgttcgac atgagcaccc
     1741 cgcttcgact tctcctctac gggctttcgg tcctggccgt attcgcggtt tccttcgtcg
     1801 ccggcaaggc cctgattccg gaggacacgg tttccgactg gacccggcag gccgatgcca
     1861 gctcggccgg gcatgaatcc atggcggggg accacgacga agcccacgtc ggcgggctct
     1921 cggtcgagca ggacggctac acgatttccc cggtcagttc cccctctgtg gccggccagc
     1981 cgggtgagct ctccttccgg atcctggacc cggaagggaa acccctgacc ggatactcga
     2041 gcaatcacga gaagcagctt cacctgatta cggttcgcct tgacggtgac cggttcaggc
     2101 acgtgcaccc cagactcgac ctggcctccg gcacgtggtc gatcccctgg tcatggccgg
     2161 ccggagggtc ctaccgggtc ttcgccgact tcgtgccgca gggatcctcc ccggatcagg
     2221 ctctgaccct gagccgcagc gttgaagtgg gcggcgaatt catgcccgag ccgaagccgc
     2281 tcagccggac cgactcgacc gggggcttca aggcaaccct tcgcggcgag ctcgaagccg
     2341 gctcgagcag cgagttgacc gcacacctga cccgcggcgg ccggccggta accgatctgg
     2401 agccgtacct cggcgccttc ggtcacct
//
LOCUS       DAOQMF010000249         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27165_length_2427_cov_3.203204, whole genome shotgun sequence.
ACCESSION   DAOQMF010000249 DAOQMF010000000
VERSION     DAOQMF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27165_length_2427_cov_3.203204"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLV77_05090"
     CDS             complement(<1..1160)
                     /locus_tag="PLV77_05090"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC15253.1"
                     /translation="MAWNIVIVGGGFAGANVARHLEKVLPRQSSRLTLVNDVNFLLYT
                     PLLPEAAAGTLEPRHVVTPLREILHRTNLRLGVVDAHDREAREVTLRSHMGQTERIPY
                     DHLVLAPGSISRFLPVPGLSEHAVGFKSLADAIFLRNHLIETLEMANATDDRQLREEL
                     LTYVFVGGGYAGLEALAELQDFAADAMRDYPKARLHGMRWILVEAADRVLPEIDQELA
                     DYAVDELRGRGIDIKLGTTLDEVTETTATISTGETVPTRTVVWTAGVVPNPSLQNFGV
                     PLDERGRVIVDSSMAVEGNEGIWSLGDSASVPNPKGGVCPPTAQHAVRQAPVVAANIA
                     AAIGLGERKIFDYRGNASFVNLGRYKAVGKIGDRTFRGFKAWWMARSYHMSQ"
     gene            1233..>2427
                     /locus_tag="PLV77_05095"
     CDS             1233..>2427
                     /locus_tag="PLV77_05095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNC15254.1"
                     /translation="MGLTSRARGGLAGALALTAALIAVSIVSSSTASAATIVNEGAPT
                     PKISKVFPNRSSVVSETIANPASADDYWTPERLASAVPAKMPNPSDALDIPDFASGAS
                     ASADGGDFTPGNVTAFPQRVHGKIFFSVGVNNFSCSGTLIDSANQSVVFTAGHCVFDQ
                     ETHGFVSNLIFLPGYENGVAQLGAANGASVFTTSQWAQSGANAYDMGVVVLDQPFEKF
                     YGSRQIAFDLNPKGLNYTIYGYPSKPNPPYNGEVLQGCHSGFAGRDDGNGNITPYPVA
                     ASPCFMQQGASGGGWVVGGNYVNSVVSYGYCDSVPSTCGFIFGPYFSNAAKSLYVQAG
                     GSPAPTIKLKRGPKGVVRKRTVNFQFTGSAATLLGFRCRFDRQKEVACSSKISIRRLS
                     PGKHTL"
BASE COUNT          417 a          786 c          778 g          446 t
ORIGIN      
        1 atctggctca tgtggtacga gcgggccatc caccatgcct tgaagccgcg gaaggtgcga
       61 tcgccgatct tgccgaccgc cttgtagcgg ccgaggttga cgaaggacgc attgccccgg
      121 taatcgaaga tcttccgttc ccccagtccg attgccgccg cgatgttggc ggcgaccacc
      181 ggcgcctggc gcaccgcgtg ctgggccgtc ggcgggcaga cgccgccctt cgggttcggg
      241 accgaggcgg aatccccgag cgaccagatt ccctcgttgc cctccaccgc catcgagctg
      301 tcgacgatta cgcggccccg ctcgtcgagc ggcacgccga agttctggag gctcggattg
      361 gggacgacgc ccgcggtcca gaccacggtc cgggtcggga cggtctcacc ggtcgagatg
      421 gtcgccgtgg tttccgtgac ctcgtcgagg gtcgtgccga gcttgatgtc gatgccgcgg
      481 ccgcgcagtt cgtccaccgc gtaatcggcc agctcctggt cgatttcggg cagcacgcga
      541 tcggcggcct cgaccaggat ccagcgcatc ccgtgcagtc gtgccttggg gtagtcgcgc
      601 atcgcgtcgg cggcaaagtc ctggagttcg gccagcgctt ccagcccagc gtatccgcct
      661 ccgacgaaga cgtaggtaag gagttcttcc cgcagttgcc ggtcatcggt ggcgttggcc
      721 atctcgaggg tttcgatcag gtgattgcgc aggaagatcg catcggccag gctcttgaag
      781 ccgaccgcat gctcgctcag gccggggacc ggcaggaagc gcgagatcga gccgggagcc
      841 agcacgaggt ggtcgtaggg aatgcgttcg gtctggccca tgtggcttcg cagggtcact
      901 tcgcgggctt cccggtcatg ggcgtcaacc actccgagcc tcaggttcgt ccggtgcagg
      961 atttccctga gcggggtcac cacatggcgc ggctcgaggg tgccggcggc ggcttcgggc
     1021 agaagcggcg tgtagagaag gaagttgacg tcgttgacca gggtcaggcg ggatgactgc
     1081 cggggcagca ccttttcgag gtggcgggcg acgttcgcac cggcgaatcc gccgccgaca
     1141 atgacgatgt tccaggccat gcggcgagta taggcagcgg accctttcgc cgaatcgcaa
     1201 ccgaaggccg tttgatctgt tctaatggac ctatgggtct cacaagcagg gcaaggggag
     1261 gattggccgg ggcactcgcc ctgaccgccg ccctcatcgc cgtctccatc gtttcttcgt
     1321 ccaccgcaag tgccgccacg atcgtcaacg aaggtgcgcc aacgccgaag atctcgaagg
     1381 tcttcccgaa cagatccagc gtggtttcgg aaaccatcgc gaacccggcc tcggccgacg
     1441 actactggac gcccgaacgg ctcgcttccg ccgtgccggc caaaatgccg aatccgtcgg
     1501 atgcgctcga catccccgat ttcgcgtctg gagcgtccgc ttccgcagat ggcggcgact
     1561 tcacccccgg caacgtgacc gcgtttccgc agcgtgtcca tggcaagatc ttcttttcgg
     1621 tcggcgtaaa caacttcagc tgctcaggaa ccctgatcga ctcggccaat cagagtgtcg
     1681 tcttcaccgc cggccactgt gtcttcgacc aggagaccca tggctttgtc agcaatctca
     1741 ttttcctgcc cgggtacgaa aacggtgttg cccagctcgg agccgcaaac ggggccagcg
     1801 tcttcactac ctcgcagtgg gcccaaagcg gggccaatgc ctacgacatg ggagtggtcg
     1861 tgctcgacca gccattcgag aagttctacg gatcccggca gatcgccttc gacctgaatc
     1921 cgaagggtct caattacacg atctatggct acccgtcgaa gcccaacccg ccgtacaacg
     1981 gcgaagtgct tcagggctgc cattcgggct tcgccggccg cgacgacggc aacggcaaca
     2041 tcaccccgta cccggtcgcg gcttcaccct gcttcatgca gcagggggcc agcggcggcg
     2101 ggtgggtggt cggcggcaac tacgtgaatt cggtcgtcag ctacggctac tgcgattcgg
     2161 taccgagcac ctgcgggttc atcttcgggc cgtacttttc gaatgccgcc aagtcgctct
     2221 acgttcaggc cggcggctcg ccggctccca cgatcaagct gaagagagga ccgaagggcg
     2281 tcgtgcgcaa gcgtacggtg aacttccagt tcaccggctc ggccgcgacc ctgctcgggt
     2341 tcaggtgcag gtttgaccgg cagaaggaag ttgcctgctc ctcgaagatc tcgatcaggc
     2401 ggctcagccc gggcaagcac acactca
//
LOCUS       DAOQMF010000250         2425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27200_length_2425_cov_4.487342, whole genome shotgun sequence.
ACCESSION   DAOQMF010000250 DAOQMF010000000
VERSION     DAOQMF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27200_length_2425_cov_4.487342"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /gene="ccsA"
                     /locus_tag="PLV77_05100"
     CDS             complement(<1..1233)
                     /gene="ccsA"
                     /locus_tag="PLV77_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNC15255.1"
                     /translation="MVLCVVVIEHAFLTDDFSFNVVATHSSIETPTFYKFTAMWSSQE
                     GSLLLWAFILGIVSTISLAATRHKLRDLVPWATAVMMGIAMFFTGLMLLGGDVNPFTT
                     ISPVPVDGTGLNPLLQHPSMIIHPPMLYTGYVSLTVPFAFAIGALISRRVDAAWIKST
                     RGFALIAWTFLSIGIILGSRWSYTELGWGGYWAWDPVENASVMPWLITTAYIHSIMVQ
                     EKRGMLKVWNVSLIVASFSLALLGTFLVRSGVLQSIHAFGDSTVGPYFLSLIGVVLIG
                     STWLIISRLDILRSDRRIDSLASREAVFLINNLLLIALCAIVFWGTFFPLISELLTGT
                     RSTLAAPWFDRYTTPVAVVLVLFTGVGPMMSWRRISWEGAKRVFTVPLIAAAVAFVLL
                     LLFTDAAESIWALLIFTLA"
     gene            complement(1367..1768)
                     /locus_tag="PLV77_05105"
     CDS             complement(1367..1768)
                     /locus_tag="PLV77_05105"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNC15256.1"
                     /translation="MDVQRKRQVRLFVALGAALLLATGLIYTSFNASSEARQPSELVD
                     AVPGKTYEMTGKVVKDSVVKTGNGIRFKVKDRDGTGEALPVTYSGVVPDPFREGREIV
                     LTGTVENGVFVGEPDTLVTKCPSKFSEKQDQ"
     gene            1829..>2425
                     /locus_tag="PLV77_05110"
     CDS             1829..>2425
                     /locus_tag="PLV77_05110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC15257.1"
                     /translation="MSEEKVLLITGASSGIGRATTRLMAGDRRLVLAGRRVEPLEELA
                     AELGGPERALVVGCDVGEWDQVETMTAAAIEAFGRIDEVFANAGFGASRGFLEESPEH
                     WKSMVQTNVLGTALTIRSTLPHLLERGSGHFIVTSSIAGRRVLPGSLYSATKFATTAI
                     GEALRQELRSRDDGKNIKVTLIEPGMVDTDFFENRPGES"
BASE COUNT          497 a          740 c          830 g          358 t
ORIGIN      
        1 ggcgagggtg aagatcagta gcgcccagat gctttccgcg gcatcggtga aaagcagaag
       61 caggacgaag gcgaccgcgg cggcgatcag ggggaccgtg aagacgcgct tcgctccctc
      121 ccaggagatc cggcgccagc tcatcatcgg cccgactccg gtgaagagga cgagcacgac
      181 cgcgaccggg gtcgtgtagc ggtcgaacca gggagcggca agggtggaac gggtgccggt
      241 cagaagctcg gagatcaacg ggaagaaggt gccccagaag acaatcgcgc aaagggcgat
      301 cagcagcagg ttgttgatca ggaacaccgc ctcgcgggag gccagcgagt cgattcttcg
      361 atccgagcgg agaatgtcga ggcgggagat gatcagccag gtcgaaccga tcaggaccac
      421 gccgatcagc gagaggaagt agggcccgac cgtgctgtcg ccgaaagcgt ggatcgactg
      481 gagcacaccg gaacggacca ggaaggtgcc gagcagggcg aggctgaaac tggcgacgat
      541 caggctcacg ttccagacct tcagcatgcc ccgcttctcc tggaccatga tcgagtggat
      601 gtaggcggtg gtgatcagcc agggcatgac cgaggcgttt tcgaccggat cccaggccca
      661 gtaaccgccc caaccgagct cggtgtatga ccagcgcgag ccgaggatga tgccgatcga
      721 gaggaaggtc caggcgatca gcgcaaagcc gcgggtcgac ttgatccagg ccgcgtccac
      781 ccggcgactg atcagggcgc cgatcgcgaa ggcgaaggga acggtcagcg acacatagcc
      841 ggtgtagagc atcggcgggt ggatgatcat cgagggatgc tgaagcagcg ggttgagccc
      901 ggtgccgtcg accggaaccg gcgagatcgt cgtgaacgga ttgacatcgc cgcccaagag
      961 catcaggccg gtaaagaaca tcgcgatgcc catcatcacc gcggtcgccc agggcacgag
     1021 gtcccgcagc ttgtgccggg tcgcggccag ggaaatcgtc gagacgatcc cgaggatgaa
     1081 ggcccagagc agcaacgagc cttcctggct cgaccacatc gccgtgaact tgtagaaggt
     1141 cggagtttcg atcgacgagt gggtggcgac cacgttgaag ctgaagtcgt cggtcaggaa
     1201 ggcgtgttcg atcacgacca cgcagagcac catcagggcg cagaaggcgt agaccgcgta
     1261 gcgggaggag tcaacccagc gtcggtcgcc cctggttccg gccagcgctg cgaccgcccc
     1321 gaatgtcgcg gtcagcaggg caacccagag cgccgcgttg cctaagttac tgatcctgct
     1381 tctccgagaa cttcgagggg cacttggtca cgagcgtgtc cggttccccc acgaagaccc
     1441 cgttttcgac agtgcccgtg agcacgattt cgcgcccttc gcggaagggg tcgggcacga
     1501 ccccgctgta ggtgacgggc agcgcctcgc cggttccgtc acggtccttc accttgaacc
     1561 tgatgccgtt cccggtcttc actacggagt ccttgaccac cttgccggtc atctcatagg
     1621 tctttccggg cacggcatcg accagctcac tgggctgacg ggcctcggaa gaagcattga
     1681 aggaggtgta aatcagtccg gtggccagaa ggagggcggc gcccagagcc acgaacaggc
     1741 gcacctgccg cttgcgctgt acatccataa cggaagggat tatcgcaagc cttggcggcc
     1801 ggcggcttcc ccctgacaga atccgcatat gagcgaagag aaggtacttc tgatcaccgg
     1861 agcaagcagc ggcatcggca gggccacgac ccgcctgatg gccggggaca ggcggctggt
     1921 tctggccggc cgccgggtcg agccgctgga ggaactggcg gcggaactgg gcgggcccga
     1981 gcgggccctg gtcgtcggct gcgacgtcgg ggaatgggac caggtcgaga ccatgaccgc
     2041 cgcggcgatc gaggcattcg gccggatcga cgaggtcttc gccaacgccg gcttcggggc
     2101 cagtcgcggc ttcctcgagg agtcaccgga acactggaag tcaatggtcc agaccaacgt
     2161 actcgggacc gccctgacca tccgttccac cctgccccac ctgctcgaac ggggcagcgg
     2221 ccatttcatc gtgaccagct cgatcgccgg ccgccgggtc ctgcccggct cgctctactc
     2281 ggcgaccaag ttcgcgacca ccgcgatcgg ggaagcgctg cggcaggaac tgcggagccg
     2341 ggatgacggc aagaacatca aggtgaccct gatcgagccg ggcatggttg acacggactt
     2401 cttcgagaac cgccccggcg aatcg
//
LOCUS       DAOQMF010000251         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27232_length_2423_cov_3.605997, whole genome shotgun sequence.
ACCESSION   DAOQMF010000251 DAOQMF010000000
VERSION     DAOQMF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27232_length_2423_cov_3.605997"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLV77_05115"
     CDS             complement(<1..611)
                     /locus_tag="PLV77_05115"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC15258.1"
                     /translation="MPATGQSGTGRQREQSEISRQKLLDAAAIVFARQGFHKASLQAI
                     GEEAGISRGSIVWHFGTKDALLEAVVERSYLSWAREVESSEAGKPGGRQAIVRVIEAH
                     RRFIEESPAENRLYYALIAEVSANEELAERVRKMHAELYLQLHGYIKEGQEAGEISSQ
                     LDPEVGARLVLSTLGGIGHLVILVPDADVGPIYREVLRSMEANL"
     gene            complement(669..>2423)
                     /locus_tag="PLV77_05120"
     CDS             complement(669..>2423)
                     /locus_tag="PLV77_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15259.1"
                     /translation="RKRVYGRFLLALMLGLAGSFALASTAQAAFGLSGLVAAPANTDA
                     GAHADFHTHLEITSPEDQLRDLTVSLPPGLSGNPQSVAKCTADQLNADSCPAGSQIGT
                     TTVNLTAMSLVPLTVNGSVYNVAPEVGEPARLGIVLRPPDPTHLLLPPIVMQVQVKLR
                     SDFGLDTILTDLPQNAAVLNLLPVGIAINSVDLDLDGAFMRNPTSCGTKTAGFSARSW
                     AMADGDPAVTGSTTFESTNCAAQSYDPQASLHIDGNGKPLAQGSRPTVTSVITQTESE
                     ANNQRADVVLPAGLGVDGGRFDQTCPLADFQAGNCPESTKVGEATAVSPLLDSDLSGP
                     VYAVGADGLPQLGVDLKGTLPLKILGSSGFRNGRVETIFEGLPDLPLSRFELVFPGGP
                     TGLIRVIGDVCAESSTFNADFTSQAGQTVSRTLPTTETGTCPSGNGGGNGNGNGNGNG
                     GNQVKKPKAKVKVSGAKNHRPRASIRVRSGGARMRVVQVKLPAALKLNKAAAKHLVAK
                     ADGKRVRGGKLVAKGRTLKLRGLPGKGAGVVTVNIPPAAIAAPGRARKGSKLTFRLKV
                     KRSGQKPVSIKASARVTK"
BASE COUNT          374 a          815 c          807 g          427 t
ORIGIN      
        1 agattcgcct ccatgctgcg gagcacttcc cgatagatcg gaccaacatc cgcatccggc
       61 acgaggatca ccaggtggcc gatcccgccc agggttgaaa gcaccagcct cgccccgact
      121 tccgggtcga gctggctcga gatctctccc gcctcctggc cttccttgat gtagccgtgg
      181 agctgcaggt agagctcggc gtgcatcttc cgcacccgct cggccagctc ctcgttggcg
      241 ctcacctcgg cgatcagggc gtagtagaga cggttctcgg ccgggctctc ctcgatgaaa
      301 cggcgatgcg cctcgatcac cctgacgatc gcctggcgac cgcccggctt tccggcttcg
      361 ctggactcaa cctcccgggc ccaggaaaga taggagcgct cgaccaccgc ctcgagcagg
      421 gcgtccttgg tcccgaaatg ccagacgatc gagccccggc tgatcccggc ctcttctccg
      481 atcgcctgaa gcgaggcctt gtgaaagccc tgccgcgcga acacgatcgc ggccgcgtcg
      541 agaagcttct gccgggatat ctcggactgc tcccgctggc gcccggttcc ggattgacct
      601 gtggctggca cgcggcaagt atcgcgactc ctccggctgc gggcggccct gccgccaccc
      661 gcggccggct atttcgtgac ccgggcggaa gccttgatgc tgaccggttt ctgccccgac
      721 cgcttgacct tcagccggaa ggtcagcttg ctgcccttgc gcgcacgccc gggagcggcg
      781 atcgccgccg gcggaatgtt caccgtcacg acaccggctc ccttgccggg cagcccgcgc
      841 agtttgaggg tcctgccctt tgccacgagc ttcccgcccc ggacacgctt gccgtcggcc
      901 ttggctacga ggtgctttgc cgcggccttg ttcagcttga gcgccgccgg cagcttgacc
      961 tggacgacgc gcatgcgggc cccgccggac ctgacccgga tcgacgcccg cggccggtgg
     1021 ttcttcgctc cgcttacctt gaccttcgcc ttgggctttt tgacctgatt gccgccgttg
     1081 ccgttgccat tgccgttgcc gttgcctcca ccatttccgc tcgggcaggt gccggtctcg
     1141 gtcgtcggca gggttcggct cacggtctgg ccggcctggg aagtgaagtc ggcattgaag
     1201 gtcgaagatt cggcgcagac atccccgatc accctgatca ggccggtcgg cccgccgggg
     1261 aagaccagct cgaaacgcga gaggggcagg tccggcagac cctcgaagat cgtctccacg
     1321 cggccgttcc tgaagccgga agagccgagg atcttcagcg ggagcgtgcc cttcaggtca
     1381 acgccgagct ggggcagtcc gtcggcgcct accgcgtaga cggggccgga cagatcagaa
     1441 tcgagcagcg ggctgaccgc cgtcgcttca ccgaccttgg tggattcggg gcagtttccg
     1501 gcctggaagt cagcgagcgg gcaggtctgg tcgaagcggc caccatcgac tccaagcccg
     1561 gccggcagca ccacgtcggc acgctggttg ttcgcctcgg attcagtctg ggtgatcacc
     1621 gaagtcaccg tcggccgcga gccctgggcc agcggtttgc cgtttccgtc gatgtgcagg
     1681 ctggcctgcg ggtcatagga ctgggccgcg cagttggtcg attcgaaggt ggtcgagccg
     1741 gtcaccgccg gatcaccgtc ggccatcgcc catgagcggg ccgagaatcc tgccgtcttg
     1801 gtgccgcatg aagtcggatt gcgcatgaag gcgccgtcca gatcgagatc gaccgagttg
     1861 atcgcgatcc cgaccgggag caggttgagc accgcagcgt tctgcggcag gtcggtgagg
     1921 atggtgtcga gcccgaagtc ggagcgcagc ttgacctgga cctgcatgac gatcggcggc
     1981 aggagcaaat gggtcgggtc cggcgggcgg aggacgattc ccagccgggc gggctcaccg
     2041 acctcgggag caacgttgta gacgctgccg ttgacggtca gcggcaccag cgacatggcg
     2101 gtcagattga cggtggtcgt gccgatctgg gaaccggccg ggcagctgtc ggcgttcaac
     2161 tggtcagcag tgcacttcgc caccgattga gggtttccgg aaagaccggg aggcaggctg
     2221 accgtcaggt cacgcagctg gtcctcgggc gaagtgatct ccagatgggt gtggaagtcc
     2281 gcgtgtgctc cggcatcggt gttggccgga gccgcgacca gcccgctcag cccgaaagcc
     2341 gcctgggctg tcgaggccag cgcaaacgac ccggccagcc caagcatgag agccagcagg
     2401 aatctcccgt acaccctctt ccg
//
LOCUS       DAOQMF010000252         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27282_length_2420_cov_4.963214, whole genome shotgun sequence.
ACCESSION   DAOQMF010000252 DAOQMF010000000
VERSION     DAOQMF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27282_length_2420_cov_4.963214"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..524
                     /locus_tag="PLV77_05125"
     CDS             156..524
                     /locus_tag="PLV77_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15260.1"
                     /translation="MTKREPKGESGAEARRQALKSGGAAVVTHRKPRAPRSDGPRTTL
                     RVPDSLVISAERLAGELGISRNDALLRLATRGADLYETERLIEKRREERWKAVVPGEI
                     EPGVSFPEAEEIAEAVRPGL"
     gene            852..1019
                     /locus_tag="PLV77_05130"
     CDS             852..1019
                     /locus_tag="PLV77_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15261.1"
                     /translation="MRQAAWLFDIQIAAVAFAAGLDVATRNIRDFEELSASLERLYPG
                     APTLAVYPDFR"
     gene            complement(1016..>2420)
                     /locus_tag="PLV77_05135"
     CDS             complement(1016..>2420)
                     /locus_tag="PLV77_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNC15262.1"
                     /translation="GPIFQSENEAARKKAADELARGSIFAFGLSERAHGADIYSTDMV
                     LTPAGDDGAGETYTATGGKYYIGNGNEAAMVSVFGRVDGIEGPDGYAFFAASSSHSNY
                     ELVRNVVNSSNYVSQFNLNAYPVESADLLHQGPGAFDAALNTVNIGKFNLGFASIGIC
                     EHAFWEAITHADSRVLYGMKVTDFPQVRQAFTDAFARTAAMKLFAWRAVDYFRTASPE
                     DRRYLLFNPLTKMKVTTEGEKVIDLLWDVIAAKGFERDMWFEMAARDIRALPKLEGTV
                     HVNMALVLKFMASYLFNPDPELAAPERRQEPGDDEFLFRQGPARGLGKIRFSPWRESY
                     EPFASVPNVAKFIELAESFKSFLVEAGPDEAQQKDLDFLLTLGEIFTLIPYGQLICEQ
                     AALIGLPEDTLDQIFDVMIRDFSAYAAELHGKTSSTEAQQEWARGAITKPVVDQARFD
                     SVWEKTRALAGGYEMNP"
BASE COUNT          429 a          734 c          798 g          459 t
ORIGIN      
        1 ggctccgagg accccgccca ggttctcgtg ctcggcaatg aagtcgaaga cccgctcggg
       61 cggaaggtcg aagtccttgt caaatcgcat gcgctgcatg gccggattga atcagattcg
      121 cggtagcgca tcaactgatg cgttatgatc gagacatgac gaaacgtgaa ccgaaaggcg
      181 aatccggagc ggaggcccgt cggcaggcgc tgaaaagcgg tggcgctgcc gtggtcaccc
      241 accgcaagcc aagagcgccg cgttccgatg gtccgcgtac tactttgcgg gttcctgact
      301 cgttggttat ttcagccgaa cggctcgccg gcgaactcgg catttcccgc aatgatgccc
      361 tgctccggct cgccacccgg ggagctgacc tgtacgaaac agaaaggctt atcgaaaagc
      421 ggcgtgagga gcggtggaaa gcggtggttc caggtgaaat agagccggga gtcagcttcc
      481 ccgaggcaga ggaaattgcc gaagcggtcc gtcccgggct ttgagttttg gtggaccgct
      541 tgtgggacac cacattcgct tctcgtatcc gcccggatag cagccttttc gactacgcgg
      601 tctccgaatc acgccagggt agaccggtac cgatcgcagc accctcgctc actgagatgt
      661 tctacgggat cgagttgaag gccatgtcag gcgatcgtcg attcggtgtc ctgcgggact
      721 ggctggttcg attgatcggc tcccaggtgt tgaacgtgat ttcactggac gggcgggccg
      781 ccgttattgc cggtcgactt cgggccgaga atccccaccc ccctccgggc cgtgattcaa
      841 ggtccaaaac gatgagacag gcagcctggc tgttcgacat tcagatcgcc gcggtcgctt
      901 tcgctgccgg gctggacgtc gctacccgga acattcgtga tttcgaggaa ctgagcgcgt
      961 cgcttgagcg gctctacccc ggcgcaccga cgctggctgt ctaccccgac tttcgctagg
     1021 gattcatctc gtagccgccg gccagggcgc gggtcttttc ccagaccgag tcgaacctgg
     1081 cctgatcgac gaccggcttg gtgatcgcgc cacgggccca ctcctgctgg gcctcggtcg
     1141 aggaggtctt gccgtggagc tcggccgcgt aggccgagaa gtcgcggatc atcacgtcga
     1201 agatctggtc gagcgtgtcc tcgggcaggc cgatcagcgc cgcctgttcg cagatcagct
     1261 ggccgtacgg gatcagggtg aagatctcgc cgagggtgag caggaaatcg aggtccttct
     1321 gctgggcctc gtccggccct gcctcgacca ggaatgactt gaacgattcg gccagctcga
     1381 tgaacttcgc cacgttcggc accgaggcga agggttcgta tgactcccgc cagggactga
     1441 agcgaatctt ccccaggccg cgcgccgggc cctggcggaa gagaaactcg tcatcacccg
     1501 gttcctgccg gcgctccggt gcggcgagtt cggggtccgg attgaacagg taggaggcca
     1561 tgaacttgag gacgagggcc atgttcacgt ggaccgtgcc ctccagcttc ggcagcgcgc
     1621 ggatgtcgcg ggcggccatc tcgaaccaca tgtcccgttc gaaccccttg gcggcgatca
     1681 catcccagag caggtcgatc accttctcgc cctcggtcgt gaccttcatc ttggtcagcg
     1741 ggttgaagag caggtagcga cgatcctcgg gggaagcggt acggaagtag tccaccgccc
     1801 gccacgcgaa gagtttcatc gcggcagtcc gggcgaaggc gtcggtgaag gcctgccgga
     1861 cctgggggaa gtcggtcacc ttcatcccgt agagaacccg gctgtcggcg tgggtgatcg
     1921 cctcccagaa ggcgtgttcg cagatcccga tcgaggcgaa gccgaggttg aacttgccga
     1981 tgttcaccgt gttgagcgcc gcgtcgaagg cgcccgggcc ctggtgaagc aggtcggccg
     2041 actcgaccgg ataggcgttc aggttgaact gggagacgta gttggatgaa ttgaccacgt
     2101 tgcggaccag ttcgtagttc gagtggctgg agctggccgc gaagaaggcg taaccgtccg
     2161 ggccttcgat cccgtcgacc cgaccgaaga ccgagaccat cgccgcttcg ttgccgttgc
     2221 cgatgtagta cttgccgccg gtcgcggtgt aggtctcccc tgcgccatcg tcaccggccg
     2281 gggtaaggac catgtcggtc gaatagatgt cggcaccgtg ggcccgctcg gagaggccga
     2341 aggcgaagat cgagcccctg gccagctcgt cggccgcctt cttcctcgcg gcctcgtttt
     2401 cggactggaa gatcggaccg
//
LOCUS       DAOQMF010000253         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27359_length_2416_cov_3.746294, whole genome shotgun sequence.
ACCESSION   DAOQMF010000253 DAOQMF010000000
VERSION     DAOQMF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27359_length_2416_cov_3.746294"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLV77_05140"
     CDS             <1..724
                     /locus_tag="PLV77_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional Fe-S cluster assembly protein
                     NifU/tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNC15263.1"
                     /translation="GAESLVTGHYARVVEDEGGALIAAGRDETKDQSYMLAALPPEVV
                     ARLKFPLADLAKDEVREIAERGGLSVARKPESQDLCFLAGQSKKDFLRRHGGLTDQPG
                     PVVDESGSELGRHPGHHHFTVGQRRGLGVAATEPLYVLGTDAKTNTVRVGSRSRLATD
                     RVRIRNAVMHRDGDRVDRVRLRYHTEPVPARVKAGAGSHEALEIELDRPFDGPAPGQA
                     AVLMSGDVVVGHATIASQRGSK"
     gene            737..>2416
                     /gene="alaS"
                     /locus_tag="PLV77_05145"
     CDS             737..>2416
                     /gene="alaS"
                     /locus_tag="PLV77_05145"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNC15264.1"
                     /translation="MKSAEIRETYLSYFEERGHRRMASASLVPPPEDTSTLLTVAGMQ
                     PFKPYFLGRETPPASRLTSSQTCFRTPDIEEVGKTLRHLTCFEMLGNFSFGDYFKEEA
                     IPFGWQLSTEGFGFDPEKIWITVFGGDDELGLGPDEEAIGIWKGLGVPDERIVKLPRS
                     ENFWQGGPTGPCGPCSEMYLDRGLDFGSVDDRPGDDSDRFLEYWNHVFMSYDQSEDGK
                     LTPLPKKNIDTGMGLERMASILQGVESVYDTDLFRPLIELAEEKSGLKYGEDEAVTRA
                     MRVISDHSRGMTFLLAAGVVPSNEDRGYVLRRVMRRAIQRGRALGLEGSWLGDFTDRT
                     IEMMGDAHPQVVAERERIDRWVRAEEESFGRTLDRGTALLEDLVDRALTEKNSWISAD
                     DAFKLHDTFGFPYDLTREMVEERGLSVDDQGFEELMEQQRNQSRSNASGGSEGADRHG
                     RILDFAGQGAESEFVGYEYMRTETGIGSLQAVNGSALAKLEQSPFYAEGGGQVADTGR
                     LIWDGGEVEVADVIRVGEDQVLELKPAAEGEAAWPPAGATVEAVVDREARFR"
BASE COUNT          426 a          784 c          849 g          357 t
ORIGIN      
        1 cggcgccgaa agccttgtta ccggtcacta cgcccgggtc gtcgaggacg aaggcggcgc
       61 cctgatcgcg gccggcagag acgagaccaa ggaccagagc tacatgctgg ccgccctgcc
      121 gccggaagtc gtggcgcggc tgaagtttcc gctggccgat ctcgccaagg acgaggtccg
      181 cgagatcgcc gagcggggag ggttgtcggt agccaggaag ccggagagcc aggacctctg
      241 cttcctcgcc ggccagagca agaaggactt cctgcggcgg cacggcggcc tgaccgacca
      301 gccgggcccg gtcgtggacg aaagcggctc cgaactcggc cggcatcccg gccatcatca
      361 cttcacggtc ggccagcgcc gcggcctcgg cgtcgcggcg accgagccgc tttacgtgct
      421 cggcacggac gcgaagacaa acacggtcag ggttggctcc cgcagtcgcc tggcgaccga
      481 ccgggtgcgg attcgcaatg cggtgatgca ccgcgacggc gaccgggtcg accgggtccg
      541 gctgaggtac cacacggagc cggttccggc cagggtgaag gcgggggcgg gcagccacga
      601 ggcgctcgaa atcgaactcg accgtccctt cgacggaccg gcacccggcc aggcggcggt
      661 gctgatgagc ggcgacgtgg tcgtcggcca cgccaccatc gccagccagc gcggatcaaa
      721 gtaggctgcg ggtctcatga agtcggcgga aatccgggag acatatctgt cctacttcga
      781 ggagcggggc catcggcgca tggcgtcggc ctcgctggtg ccgccgccgg aggacacctc
      841 gaccctgctg accgtggccg ggatgcagcc cttcaagccg tacttcctcg gccgggaaac
      901 tccgcccgcc tcgcggctca cctcgagcca gacctgtttc cgcaccccgg acatcgagga
      961 agtcggcaag accctcaggc acctcacctg cttcgagatg ctgggcaact tcagcttcgg
     1021 cgactacttc aaggaagagg cgatcccctt cggctggcag ctttcaaccg aagggttcgg
     1081 attcgacccg gaaaagatct ggatcaccgt cttcggcggg gacgacgagc tcgggctcgg
     1141 acccgacgag gaggcgatcg ggatctggaa gggcctcggg gtcccggacg aacggatcgt
     1201 gaagctgccc cgctccgaga acttctggca gggcgggccg accggcccct gcggcccctg
     1261 ctccgagatg tatctggacc gcggcctcga cttcggttcc gtggacgacc gtcccggcga
     1321 tgacagcgac cgcttcctcg agtactggaa ccacgtcttc atgagctacg accagtccga
     1381 ggacggcaaa ctgaccccgc tgccgaagaa gaacatcgac accggcatgg gcctcgagcg
     1441 catggcctcg atcctgcagg gtgtggagtc ggtctatgac accgacctct tccggcccct
     1501 gatcgaactg gccgaggaga agtccggttt gaagtacggc gaagacgagg ccgttacaag
     1561 ggcgatgcgg gtgatcagcg accacagccg cggcatgacc ttcctcctcg ccgccggggt
     1621 ggtgccctcg aacgaagacc gcgggtacgt gcttcggcgg gtgatgcggc gggcgatcca
     1681 gcgtggccgg gcgctcggtc tcgaaggctc gtggctgggc gacttcaccg accgcacgat
     1741 cgagatgatg ggtgacgccc acccgcaggt ggtggcggag cgggagcgga tcgatcgctg
     1801 ggtcagggcc gaggaggaga gcttcggtcg caccctcgac cgcggcaccg ccctgctcga
     1861 agacctggtc gatcgggccc tcacggagaa gaactcgtgg atcagtgccg atgacgcctt
     1921 caagctccat gacactttcg gcttccccta cgacctgacc cgagagatgg tcgaggagcg
     1981 gggcctttcg gttgatgacc agggcttcga agagttgatg gaacagcagc gcaaccagtc
     2041 ccgcagcaac gcctccggcg gctccgaggg ggcggatcgt cacgggcgga tcctcgactt
     2101 cgccggccag ggcgccgaat cggagttcgt cggctacgag tacatgcgga cagaaaccgg
     2161 aatcgggtcg cttcaggcgg tcaacggctc ggctctcgcc aagctcgaac agagtccctt
     2221 ctacgccgag ggtggcggcc aggtcgccga caccggccgg ctcatctggg acggtggcga
     2281 ggtcgaggtc gccgacgtga tccgggttgg tgaagaccag gtgctggaac tgaagccggc
     2341 cgccgaaggc gaagctgcct ggccgccggc cggggcgacg gttgaagcgg tggtcgaccg
     2401 ggaagcccga ttccgg
//
LOCUS       DAOQMF010000254         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27432_length_2413_cov_3.067430, whole genome shotgun sequence.
ACCESSION   DAOQMF010000254 DAOQMF010000000
VERSION     DAOQMF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27432_length_2413_cov_3.067430"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="PLV77_05150"
     CDS             <1..1356
                     /locus_tag="PLV77_05150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNC15265.1"
                     /translation="KANGLDFEGLVVKAIELLGNQRIRTGWRNGISAVLVDEFQDVSE
                     AQFELLRLLAGDDGAGLSVIGDEDQSLYSWRGASTALLTGFEQHFPQCQVINLACNYR
                     SHEAIVGTARRLIEHNEGRRGKELVAHREEGAMPVHQRFDSEEAEALAIVAWAKEILR
                     CGESPAEIAVLTRVRGLPLKNELERLLAGAGITYHAVGATTLWERSEVRDVLALFHLL
                     ANAKHLPAFARVFEQIKGIGAKATDTVVQAARKEGVSPVEICRDGTVNLGPKRNTAVR
                     EFVARFDQVNSLDPDKLGFAGWVGQVIADWGFSEAWRADKDGRRKRESVIQLYRAAEE
                     FEARTSMELTPPKLSDWLNELSLHAPQSGKDAERQGLALATIHASKGLEFSHVWLAGF
                     DEGVLPSNRSLEEGREDEERRLAYVAITRAKETLTVSSSEYRRGQESQISRFAAECGL
                     A"
     gene            1443..>2413
                     /locus_tag="PLV77_05155"
     CDS             1443..>2413
                     /locus_tag="PLV77_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HNC15266.1"
                     /translation="MSESNGGTNGAAAVADPGSAQAAIQAVANVAAGQRPFTIPAELA
                     KKIAQARKGIGAVAKNGHNADQHYNYVQIEDVVQEAGRALAEASVIVMPGEPIRMETE
                     DLRSGGGASGKLITVTFLVRVIDGETGEGYETEQIGMASDYPGDKALAKARSSMKKYF
                     LADLLEIPLGDGTDAETTNHGKGGAKIGDTKPASDKQKDLVKKLIGEKNLPKPAKAAI
                     VNFVGGSEPKKGPTSHAIDQLMNHDPLAFARECGWDGTVEEAATEPQPAPAEAPAEPE
                     AAAPQPSGEAMALPEAEGEALVLFCREKGATAGELATVLRSIGINLPP"
BASE COUNT          565 a          727 c          768 g          353 t
ORIGIN      
        1 aaagcgaacg gactcgactt cgaagggctc gttgtgaaag caatcgagct gctcggcaac
       61 cagaggatcc ggaccggctg gcgaaacggc atcagtgccg tgctcgtcga cgagttccag
      121 gacgtatccg aagcccagtt cgaactgctc cggctgctgg ctggtgacga cggtgccgga
      181 ctatcggtga tcggtgatga agaccagtcg ctttactcat ggcgcggagc atcaaccgcg
      241 ctactgaccg gcttcgaaca gcatttcccg caatgccagg tgatcaacct tgcctgcaac
      301 taccgctcgc atgaagcgat cgtcggcacg gcccgtcgcc tgatcgaaca caacgagggg
      361 aggcgaggaa aagaactcgt cgcccaccgg gaggaaggag ccatgccggt tcaccaacga
      421 ttcgactcgg aagaagccga ggcactcgcg attgtcgcct gggccaagga aatcctcaga
      481 tgtggcgaat cgccggccga gatcgcggta ctcacaaggg ttcgtggcct gccactcaag
      541 aacgagctgg aaaggctgct ggccggtgcc ggaatcacct accacgcggt aggggcaaca
      601 accctctggg aacgctccga agtgcgggac gtgctcgcgc tgtttcacct gttggccaat
      661 gccaagcatc tgccggcgtt cgcccgggtg ttcgaacaga tcaaagggat cggggcgaaa
      721 gccaccgaca ctgtcgtgca ggccgcccgg aaagaaggag tcagcccggt ggagatctgt
      781 cgggacggca ccgtcaacct cggaccgaaa agaaacacag cggtcaggga gttcgttgcc
      841 aggttcgatc aggtcaattc gcttgacccg gacaagctcg ggttcgccgg ctgggttggc
      901 caggtgatcg ccgactgggg attctccgaa gcgtggagag ccgacaagga cggacgcaga
      961 aagcgcgaat cggtgattca gctgtaccga gcggcggaag agttcgaagc ccgcaccagc
     1021 atggaactaa ccccgccgaa actcagcgac tggctcaacg aactgtcgct acacgcacca
     1081 caatccggca aggacgccga acgtcaggga cttgccctgg caaccatcca tgcctccaag
     1141 ggcctggagt tctcccatgt ctggctggcc ggttttgacg aaggcgtttt gccttcgaac
     1201 cggtcgctgg aagaaggcag ggaagacgaa gaacgacggc tcgcctacgt cgccatcacc
     1261 cgcgcaaagg aaacactgac cgtttcctcg agcgagtacc ggcgcggcca ggaaagccaa
     1321 atctcccggt tcgcagcgga atgcggcctt gcctgacagc aagcgtccaa atgacgccgc
     1381 ccctgggggt acattgaaaa gcgtccgagg tagctcctcg gcaaacgaaa ggaagatgtc
     1441 aaatgagtga atccaacggt ggcaccaacg gtgccgcagc ggtagccgat cccggctccg
     1501 cacaggcagc aatccaagca gtagcgaacg tcgcagcagg acagcgcccg ttcaccattc
     1561 ccgcggagct cgcgaagaag atcgcgcagg cccggaaggg aatcggtgct gtcgccaaga
     1621 acggccacaa cgccgaccag cactacaact atgtccagat cgaggacgtt gtgcaggagg
     1681 ccggaagggc tttggccgag gcctcggtga tagtcatgcc cggggaaccg atccggatgg
     1741 aaaccgagga tctgaggtcc ggtggcggtg ccagcggaaa gctgatcacg gtcaccttcc
     1801 tggtccgggt gatcgacggc gaaaccgggg aaggatacga aaccgagcag atcggcatgg
     1861 caagcgacta tcccggggat aaggcactcg ccaaggcacg aagctcaatg aagaagtact
     1921 tcctggcgga cctgctcgag atccctctgg gagatggcac tgatgcggaa accaccaacc
     1981 acggcaaggg tggcgcgaaa atcggcgaca ccaagcccgc atccgacaag cagaaggatc
     2041 tcgtcaagaa actgatcggc gagaagaacc tgccgaaacc ggcgaaagcc gcgatcgtca
     2101 acttcgtcgg cggaagcgaa ccgaagaagg gtccgaccag ccacgcgatc gaccagttga
     2161 tgaaccatga cccgctcgcg ttcgcccgcg aatgcggctg ggacggaacg gtcgaagaag
     2221 ccgcaaccga accccagccg gccccggccg aggcgcctgc ggaaccggag gccgcggcac
     2281 cccagccaag cggggaagcg atggctcttc ccgaggctga aggtgaagcg ctggtcctgt
     2341 tttgccggga gaagggtgcc accgccgggg agctcgcaac cgtgctccgt tcgatcggca
     2401 tcaacctgcc acc
//
LOCUS       DAOQMF010000255         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27542_length_2408_cov_2.888228, whole genome shotgun sequence.
ACCESSION   DAOQMF010000255 DAOQMF010000000
VERSION     DAOQMF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27542_length_2408_cov_2.888228"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLV77_05160"
     CDS             <1..924
                     /locus_tag="PLV77_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15267.1"
                     /translation="VEAKLKQEREHLAEVRARLHRALKILSDQLVSLYVNGSPDVEEM
                     VLASSDFGELVSSAGYADAIQDRNESVITRVEDLRDQIKVMVEKMKVQQKKLEAARDE
                     IAAETKQAEDARDAVEAQKAEFEASSASRQAKIDALEAKAGSLEDSLPDLTMDPASSS
                     APRAVPPVAGSVAVINSDGTASPPADAPQAVKDVIAAGNQIIDLPYVWGGGHGSFESS
                     GYDCSGTVSYALHGGGLISSPLDSTGLTTWGEPGEGNWITVYGNSGHAFMHVAGISLD
                     TSGTDGTGPSWSSDLGWEDTSAFVARHPSGL"
     gene            complement(1328..2113)
                     /locus_tag="PLV77_05165"
     CDS             complement(1328..2113)
                     /locus_tag="PLV77_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNC15268.1"
                     /translation="MTDQNPSDDAAMPESGAAAPESEHRLRESVKTELTGGTQPDNGV
                     EGPRAIKAERIMNGPLMVAAALTLPSVALTEAHMGGPLEVIAQILNWGTWIAFAIEFV
                     VMLALVPDRGKYLRHHPVEIIVVFLTPPVLPPSLQSLRAIRLLRLLRLLKLAQLSNRV
                     FSNEGLQYAALLTVLATVAGGSVFRAFEAKNQHLTEWDGIYWAFTAMTTVGSDFSATT
                     IGGQVTEVVILLVGISFIAMLTGAIAQRFLRPPGTNPKPQDPS"
BASE COUNT          477 a          739 c          778 g          414 t
ORIGIN      
        1 gttgaagcca aactgaagca ggagcgggag cacctcgccg aagttcgcgc ccggctgcac
       61 cgcgccctga agatcctttc ggaccagctc gtctcgctct acgtcaacgg ctccccggac
      121 gtcgaggaga tggttctggc ttcctcggac ttcggtgaac tggtctccag tgccggatac
      181 gccgatgcga tccaggatcg aaacgagtcg gtgatcaccc gggtcgagga ccttcgcgac
      241 cagatcaagg tgatggtcga gaagatgaag gtccagcaga agaagcttga agcggcccgc
      301 gacgagatcg cagccgaaac caagcaggcc gaggacgccc gtgacgcggt cgaggcccag
      361 aaggccgaat tcgaggcctc cagcgcctcc cgccaggcga agatcgacgc gctcgaagcc
      421 aaggccggtt cgctcgagga ctcgctgccc gacctgacca tggacccggc ctcaagttcg
      481 gctccccggg cggtcccccc ggtcgcggga tcggtcgccg tgatcaattc cgacggcacg
      541 gcatcgccac ccgctgacgc tccgcaggcg gtcaaggacg tgattgccgc gggcaaccag
      601 atcatcgacc tcccctacgt gtggggcggc ggtcacggat ccttcgaatc gtccggttac
      661 gactgttccg gcacggtcag ttacgccctg cacggcggcg gcctcatttc cagcccgctc
      721 gactcgaccg gcctcaccac ctggggcgaa ccgggcgaag gcaactggat caccgtttac
      781 ggcaattcgg gtcacgcctt catgcacgta gcgggaatca gcctcgacac ctccggtacc
      841 gacggcaccg gccccagctg gtcaagcgac ctcggctggg aagacacctc ggccttcgtg
      901 gcccgccacc cctccggcct ctagcccggg ccccgctcaa cggctcgatc aacccgtggg
      961 acgtgacttt ctgccgtaaa gcggcagttg ctcgtacctc gatgcctccc gaaggaccca
     1021 agtctgaaag ggccagtgag ggcaaccttt tgggcgtctc gaaattcgag atggagaatg
     1081 ttgccgaata gcgcacccaa ctccgatctc gattccccgg ttcaggacga gatcggagtt
     1141 gagggcgcta tttgccgaaa acttcaatgt cgtttgtgaa ttccgcagcc ggcacgggct
     1201 cctcaggtga cccagcaatc cctcctccaa tgccttcccg ctaccgtggg tcggattttc
     1261 cgcgtcaggg acggaaatcc gacccagtgg gctcaggtga cgggcgtgaa ctggtcggct
     1321 gagctgttta gcttgggtcc tggggtttgg ggttggtacc ggggggtctc aggaacctct
     1381 gggcgattgc gccggtgagc atggcgatga aggagattcc gacgaggaga atcacgacct
     1441 cggtgacctg accgccgatt gtcgtggccg agaagtccga gccgaccgtg gtcatcgcgg
     1501 tgaaggccca gtagatcccg tcccattcgg tgaggtgctg gtttttggct tcaaatgcgc
     1561 ggaacaccga accgccggcg accgttgcca gcacggtgag gagggccgca tactgaagtc
     1621 cctcgttcga gaaaacacga ttggagagct gggccagttt gagcagacgg agcagtcgca
     1681 acaacctgat cgcccgcagg ctctgaaggc tcgggggcag gaccggggga gttaggaaga
     1741 cgacgatgat ttcgaccggg tggtgcctca ggtacttgcc tcgatccggc accagggcga
     1801 gcattacgac gaactcgatc gcgaaggcga tccaggttcc ccagttgaga atctgggcga
     1861 tcacctcgag ggggccaccc atgtgagcct cggtgagggc gacgctgggg agggtgaggg
     1921 cggccgcgac catgagcggc ccgttcatga ttcgttcggc cttgatcgcg cgggggccct
     1981 cgacgccgtt gtcgggctgg gtgccgccgg tcagctcggt tttgaccgac tcgcgaagtc
     2041 gatgttcact ctcgggcgcc gcagccccgg attcgggcat cgcggcgtcg tctgacgggt
     2101 tctggtcggt catcggagcc gtcttcgacg cgagggctgt cgtgcctgta aacgcagcag
     2161 aaatcccggt tgcgacctcg ggaacggtcg ggaaggaaac cagaccggga cggatcgatt
     2221 gacttacaga aagtaattcg ctaaactaat tagcagagca aacaaacccc tcggagcggc
     2281 tccctgaacg cccaagccag gtcgagacac cgaaccggaa caaagaacgt ggaatcaagt
     2341 gacagagcca gatacaagta gtcagacgag cagccagaca aggcgtcaga cgacagtcgc
     2401 cgaggttg
//
LOCUS       DAOQMF010000256         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27805_length_2395_cov_3.022650, whole genome shotgun sequence.
ACCESSION   DAOQMF010000256 DAOQMF010000000
VERSION     DAOQMF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27805_length_2395_cov_3.022650"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1296)
                     /locus_tag="PLV77_05170"
     CDS             complement(13..1296)
                     /locus_tag="PLV77_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC15269.1"
                     /translation="MLRAAVASAAIALLVADHYLHMDHWVIICSALVLLVLICGGLVI
                     AIGSARRNVRSEALNRIKEEENLHLADIVRSSKDAIFSVDLDGKVTAWNEGAHNLYGY
                     SAEEAIDRRLTDLTIPAERSKELGATFRRVISGGWQEFESERITKSGERIRISSRTFP
                     VRDGEGNVIGMSISTHDMTGPELPGPEMEQDLLWQSRLREALEKSSFVFWGQPVFDAR
                     SGSVDHTELLIRMDLNGEIIAPEEFLPHAEASGLIDRIDAWAIREGIRIAAGRPVAIN
                     LSGRSVSASETGKIISESLDDSRTDPRNVRFEITETAAIENLDAARSLVVELKKMGCA
                     VSLDDFGTGYGSFTYVRQLPPVDLKIDSSFVMDLDSEEASRRVVSSIVSISKTFGVST
                     VAEGIEDEATLELVKGMGINFLQGFHLGTPQPLDR"
     gene            complement(1434..>2395)
                     /locus_tag="PLV77_05175"
     CDS             complement(1434..>2395)
                     /locus_tag="PLV77_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC15270.1"
                     /translation="VDEPFPTLKSFNSITLRVILRAVFGAEGKELAELEQIIPPITRY
                     GQMRVASSLLMRDYGRFSPGGRGERLMTRFHEIIRTLIDKHLADPGLADRIDILALML
                     NSDLQEGRAVDYQATGEELMTILVAGHETTASSLAWNIERITRHPEVLDRLTAEARTD
                     QNEYRLAVINEVLRTRPVIGMTGRSVFAESFELDGWKLPAGTRILCNIRETNVDPSAH
                     PEADRFNPDRYVGKKPDTYSWIPFGGGLRRCLGAAFAQMEMDIVLRTLLRRFDLAATS
                     RPPEGERFKGLAYAPSRGGVAMVRYRDGKPPARLEPQVAAGAR"
BASE COUNT          435 a          747 c          764 g          449 t
ORIGIN      
        1 aaaccgccgt tcctaccggt cgaggggctg aggagtaccg aggtgaaagc cctggaggaa
       61 attgattccc atgcccttga cgagttcgag cgtggcctcg tcctcgatgc cttcggccac
      121 ggttgagaca ccaaaggtct tcgagatcga aacgatcgaa ctcaccaccc tgcggctggc
      181 ctcctccgaa tcgagatcca tcacgaacga ggaatcgatc ttcaggtcga ccgggggaag
      241 ctgccggacg taggtgaagg aaccgtaacc cgtaccgaaa tcgtcaagcg acaccgcgca
      301 gcccatcttc ttcagttcaa cgaccaggga acgggcggcg tcgaggttct cgattgccgc
      361 agtctcggtg atttcgaaac ggacgttcct cgggtcggtg cgggagtcat cgagggattc
      421 ggagatgatc ttcccggtct cgctggccga gacgctgcgc ccggacaggt tgatcgcgac
      481 cggcctcccg gccgcgatcc ggatcccttc ccggatcgcc caggcatcga tccggtcgat
      541 caggcccgaa gcttcggcgt gtggcaggaa ttcctccgga gcgatgatct ccccgttcag
      601 atccattcgg atcagcagtt cggtgtgatc gacggaacca ctgcgggcgt cgaagacggg
      661 ctggccccag aacacaaatg aagacttctc cagcgcttcc cgaaggcggg attgccacag
      721 caggtcctgc tccatttccg gacccggcaa ctccgggccc gtcatgtcgt gggtgctgat
      781 cgacatgccg atgacgtttc cctcgccgtc ccggaccgga aaggtccggc tcgaaatccg
      841 gattctttct cccgacttgg tgatccgttc cgattcgaac tcctgccagc cgccgctgat
      901 cacgcgccgg aaagtcgctc cgagttcctt tgaacgctcg gccgggatgg tcagatcagt
      961 gagccggcga tcgatcgctt cctcggccga gtagccgtac aggttgtgcg ccccttcatt
     1021 ccaggcggtc accttgccgt cgaggtcaac cgagaagatt gcgtccttgg aggatctgac
     1081 gatgtcagcg aggtggaggt tctcttcctc cttgatccgg ttcagggctt cgctgcggac
     1141 attccggcgg gcggaaccga ttgctatgac caggccgccg cagatcagga cgagcaggac
     1201 cagcgccgaa cagatgatca cccagtgatc catgtgaagg tagtggtcgg caaccagcaa
     1261 tgcgatcgcg gcactcgcga ccgctgcacg cagcacggca aaccgtgccc cgaacggatc
     1321 cgttcgaaag agtgccctca ctgatgcgga tcttggttgc cgtttcaaaa ttgctcgatc
     1381 gacgagcgtg ggcgacgccc cctgaggctc agctaaaaga taaccgcgat ggcctagcgt
     1441 gctccggcgg ccacctgcgg ctccagccgg gcgggcggct tgccgtcgcg gtagcggacc
     1501 atcgccaccc cgcccctgga cggggcgtag gcgagtccct tgaacctctc gccttcgggc
     1561 ggccggctgg ttgcggcgag gtcgaaacga cgaagcaggg tcctgagcac gatgtccatt
     1621 tccatctggg cgaaggcggc gccaaggcag cggcgcagcc cgcccccgaa gggaatccag
     1681 gaataggtgt cgggcttctt gccgacgtag cggtccgggt tgaagcgatc ggcttcgggg
     1741 tgagctgaag gatccacgtt ggtttcccgg atattgcaga ggatcctggt gccggccggc
     1801 agcttccagc catcgagctc gaagctctcg gcgaagaccg aacgcccggt catgccaatc
     1861 accgggcggg ttctgaggac ttcgttgata accgcaagcc ggtactcgtt ctgatcggtc
     1921 cgggcttcgg cggtgagccg gtccagcacc tcggggtgcc tggtgatgcg ctcgatgttc
     1981 caggccagtg aggaagcggt cgtttcgtga ccggcaacca gaatggtcat cagctcttcc
     2041 ccggtcgcct ggtaatcgac ggctctcccc tcctgcaggt ccgagttcag catcagggcg
     2101 aggatgtcga tccggtcggc gaggccgggg tcggccaggt gcttgtcgat cagggtccgg
     2161 atgatttcgt ggaaccgggt catcagccgc tctccgcgac cgcccgggct gaaccgcccg
     2221 tagtctcgca tcagcagact ggaggcgacc cgcatctggc cgtaacgggt gatcggcggg
     2281 atgatctgct ccagttcggc aagctctttg ccctcggccc cgaacaccgc ccggagaatg
     2341 acccgcaggg tgatcgaatt gaaggacttc aaggtcggga acggctcgtc gaccg
//
LOCUS       DAOQMF010000257         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_27839_length_2393_cov_3.896065, whole genome shotgun sequence.
ACCESSION   DAOQMF010000257 DAOQMF010000000
VERSION     DAOQMF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27839_length_2393_cov_3.896065"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..757
                     /locus_tag="PLV77_05180"
     CDS             92..757
                     /locus_tag="PLV77_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC15271.1"
                     /translation="MKEAGKKPFSYMTLVGVIFLVVVLVALFNMLKTTDSGTVGIGEV
                     GIGDRAAPFAVPLAMSGLDGDANIDPDEACSVEGADVLRICDYFERPLVMSFWFTKGA
                     SSCIDQQDVFDEVAAKYRRQAGFVSINVRDDRDRVRELIGEHGWKVPVGYDRDGAVSN
                     IYRVGGCPTFLFFRKGGVLEDAEIGENDAGQLGAQVRSLIDGESKATQQESASDPESG
                     PTG"
     gene            783..1241
                     /locus_tag="PLV77_05185"
     CDS             783..1241
                     /locus_tag="PLV77_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15272.1"
                     /translation="MESTTRQSGPAERRESRTRFGKWADRLEETADERPPAPWGSFPI
                     GAVSVFVGLVLAVIGLINANPVQLAIGVGLGMLGGLELAIREHFTGFRSHTTLLAGFV
                     FVVAGGATFYAAHWILWQSLLLGAALFGATFWLLRKKFEKASGGLSYKLR"
     gene            complement(1344..1682)
                     /locus_tag="PLV77_05190"
     CDS             complement(1344..1682)
                     /locus_tag="PLV77_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15273.1"
                     /translation="MEVAATADSKSIASRPMPQHLQALERANRVRLARAALKRSIASG
                     EVSVTKVITDCPWQTETMTLSELLRAQSRWGRTRTRKLLASVGLSENKRLDTLTERQR
                     MLLVSQLRPH"
     gene            2020..>2393
                     /locus_tag="PLV77_05195"
     CDS             2020..>2393
                     /locus_tag="PLV77_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15274.1"
                     /translation="MSKHVKLLLGLGALAIVAMVIVACGSSDDSSDDSGNVAGVSADT
                     CGEMTYGGEGSPDALIVSDLPLQGDSADRSKQMNDAVTQVLDTAGWKAGNTTIGFQAC
                     DDSLADTGLWDEQTCKDNATAY"
BASE COUNT          398 a          729 c          832 g          434 t
ORIGIN      
        1 acttcggcct gatcgcggtg atcgatctcg gcgttgcgct ggccggagtg gcgctggtcc
       61 tgccggcgat gctggtctgg ctggagcgcc gttgaaggaa gccgggaaga agcccttcag
      121 ctacatgacc ctggtcgggg tcatcttcct ggtggtcgtc ctggtcgcgc tcttcaacat
      181 gttgaaaacc accgactcgg gcacggtcgg gatcggggag gtggggatcg gtgaccgggc
      241 ggctcccttc gccgtgccgc tggcgatgtc cggactcgac ggggacgcca acatcgaccc
      301 ggacgaggcc tgctcggttg aaggtgccga cgtgctgagg atctgcgact acttcgagcg
      361 gccgctggtc atgagcttct ggttcaccaa gggcgcttcg agctgcattg accagcagga
      421 cgtattcgac gaggtcgccg cgaagtaccg ccggcaggcg ggctttgttt cgatcaacgt
      481 ccgcgacgac cgtgaccggg tccgggaact gatcggcgag cacggctgga aggttccggt
      541 cgggtacgac cgggatggcg cggtttccaa catctaccgg gtcggcggct gcccgacctt
      601 tctcttcttc cgcaagggcg gggtgctcga agacgccgag atcggcgaga acgacgccgg
      661 acagctgggg gcgcaggtac gctccctgat cgatggcgag tcgaaggcaa cgcagcagga
      721 aagcgcgtcg gacccggaat ccggtcccac cggctgaggc ggcccgggcc gacgccgggt
      781 ccgtggaaag cacgacgcgg cagtccgggc ctgccgagcg ccgcgagtcg cgcacgaggt
      841 tcggcaagtg ggccgaccgg ctggaggaaa ccgcggacga gcggccgccc gcaccctggg
      901 gcagctttcc gatcggcgcg gtctcggtct tcgtcgggct cgtgctcgca gtgatcggcc
      961 tgatcaacgc caacccggtt cagcttgcga tcggagtcgg cctcggcatg ctgggcgggc
     1021 tcgaactggc gatccgcgag cacttcacgg ggttccggtc acatacgacg ctgctggcgg
     1081 gtttcgtctt cgtggtcgcg ggcggggcga ccttctacgc ggcccactgg atcctctggc
     1141 agtcgctcct gctcggcgcg gcactgttcg gagccacctt ctggctgctc aggaagaagt
     1201 tcgaaaaggc ctccggaggc ctcagctaca agctccggtg actcaactgc agtcgggcgg
     1261 ggtccaaccc gccctcccag tagaactcgc cccttccccc gaaaaaaaga atggagggaa
     1321 ggggctcagt ctggtggtgc ctctcagtgg gggcggagct ggctgacgag cagcatgcgc
     1381 tggcgctcgg tcagggtgtc aaggcgcttg ttctcgctca ggccgaccga ggccaggagc
     1441 tttctcgtcc gggtccggcc ccagcgggac tgggcgcgga gcagttccga gagggtcatc
     1501 gtctcggtct gccagggaca gtcggtgatc actttggtaa cggaaacttc accgctggcg
     1561 attgatcgct tcagggctgc gcgagcaagt cgaacccgat tggcccgttc gagagcctgc
     1621 agatgctggg gcatcggacg gctggcaatc gacttagaat cggcggtggc ggcaacttcc
     1681 acgggcggtc ctcccttcgg gggattggtc ttgttgactg gatggcatgg ggcgacaggc
     1741 cagggatcct ctttcccggg tggcgcttct cccccgcctg gtgccgggct ttccgtccga
     1801 ctcaggcgat aaaggcaaca tagacaagtt ggccggaccg ggcaatggtc agggccgcga
     1861 actttcgcta agagcaggtt tttctttttc gccgaattcc cttgtcgggg acctgcccgg
     1921 aatgtatggt cgaggcacgg tctgaccgaa cactgttcgg tcggcgcaag gttcaagtgg
     1981 cctccagtcc gacccggcca cctacagacg ggagagagag tgtccaagca cgtgaagctt
     2041 ctactcggtc tcggcgccct ggccatcgtg gccatggtca tcgttgcctg cggcagtagc
     2101 gacgactcat cggatgattc cggaaacgtt gccggcgtga gcgccgacac ctgcggtgag
     2161 atgacctacg gcggcgaggg ttcacccgat gccctgatcg tctccgatct gccgctccag
     2221 ggtgattctg cggatcgctc gaagcagatg aatgacgcgg tcacccaggt gctcgacacc
     2281 gcgggatgga aggccgggaa cacgacgatc ggcttccagg catgtgacga ctcactcgcg
     2341 gacaccggac tgtgggatga gcagacctgc aaggacaacg ccaccgccta cag
//
LOCUS       DAOQMF010000258         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28052_length_2383_cov_3.008591, whole genome shotgun sequence.
ACCESSION   DAOQMF010000258 DAOQMF010000000
VERSION     DAOQMF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28052_length_2383_cov_3.008591"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLV77_05200"
     CDS             complement(<1..531)
                     /locus_tag="PLV77_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNC15275.1"
                     /translation="MAEAAQPISPQGAKARVLVAGATGYAGALAAGLVWKHPRLELVA
                     VTGRSETGRKLSDLYPRYPVPLELKELEDSDFEGVEVGIVAYPHGAAAPVVAAMRGRG
                     LLVVDLSADFRFRDLPVYEEFYGPHGEPGLLDGAAYGLTELDREWIREAELVANPGCY
                     PTASILALAPLARAGLI"
     gene            complement(524..>2383)
                     /gene="pheT"
                     /locus_tag="PLV77_05205"
     CDS             complement(524..>2383)
                     /gene="pheT"
                     /locus_tag="PLV77_05205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNC15276.1"
                     /translation="TADRVRILVQDEDLCPRFTARGYSEVKVGPSPTWLKARLIAAGM
                     RPISNVVDITNYVMWLTGQPMHAYDLDLVPEGELIVRRAENGEKLAALDGSEYELTSE
                     MVAVADRGGLAGIGGMMGGSVSEVSESTTTVLMESANWNGPNILKTSRDLGLRSEASS
                     RFEKQLHPDLTLRAQAVAARLMVEICGAVPMTGVVDVASGSPASAPIRLRAGRVERIL
                     GLPIEADRQAEALEMLGFGVEREGEDLLVTVPPDRYFDVTREIDLVEEVGRISDLDRN
                     LPATLPSGAGRPGGLSRQQALQRRAEDSLRESGFDEIVGWSFTDPGEKDRLRLAPEDP
                     RAQAVKLANPLSEDQSVMRTTLLGSVLGAARRNLSRGTDRVALFESGRVYLPSGAFGP
                     GPLGGDFPGNTRPPSNEPQHLCALAVGSIDPSSWREPAAGSDFFALKGVLEHLAGGLG
                     TAVTVERIPGAPTQPFLHPGKSGVVAVGGQPIGWIGEIHPLVAADYDLDSAVAFEIAL
                     ADLLQSSPVGEEAYEDFTPFPPVDRDLAVLVPDAEPSAGVLATVQQAGGELLTGVSVF
                     DVYSGEGVPEGEKSLALRLRFRAPDRTLSDEEIEPLWSGIIAAIESAGGKLRG"
BASE COUNT          390 a          837 c          805 g          351 t
ORIGIN      
        1 gatcagcccg gcccgggcca gaggcgccag ggcgaggatc gaagcggtcg ggtaacaacc
       61 gggattggcg accagctcgg cttcgcggat ccattcgcgg tcgagctcgg tcaggccgta
      121 ggcggcgcca tcgagcagtc cgggctcgcc gtggggtccg tagaactcct cgtagaccgg
      181 caggtccctg aaacggaagt cggccgagag atcgaccacc aggaggcccc ggccccgcat
      241 cgcggccact accggggcgg cggcgccatg cgggtaggcg acaatcccga cctcgacccc
      301 ttcgaagtcc gaatcctcca gctccttcag ctccagcggc accgggtagc gcgggtagag
      361 gtcactcagc ttccggcccg tttccgagcg accggtgacc gcgaccagtt ccagccgcgg
      421 gtgtttccag accagcccgg ccgccagcgc gccggcgtat ccggtcgctc cggcgaccag
      481 cacccgggct ttcgcccctt ggggggagat cggctgtgcg gcctcagcca cgcagcttcc
      541 ctccggccga ttcgatcgcg gcgatgatgc ccgaccagag cggctcgatc tcctcgtcgc
      601 tgagggtccg gtccggcgcc cggaagcgca gcctcagggc gaggctcttc tcgccctcgg
      661 gaacgccttc gccgctgtaa acatcgaaaa cggagacccc ggtgagaagt tccccgccgg
      721 cctgctgaac cgtggccagg acgccggcgg aaggttcggc gtcaggaacg agcaccgcca
      781 ggtcacggtc gaccggcggg aacggtgtga agtcctcgta ggcctcctcg ccgaccggac
      841 ttgactgaag caggtcggca agggcgatct cgaaggcgac cgccgagtca aggtcgtaat
      901 cggcagcgac caggggatgg atctcgccga tccagccgat cggctgaccc ccgaccgcga
      961 ccactcccga tttgcccgga tggaggaagg gctgcgtcgg ggcgcccggg atccgctcca
     1021 cggtcaccgc ggtgccaagc cctccggcca ggtgttcaag cacgcccttg agagcgaaga
     1081 aatcggatcc cgcggccggt tcgcgccagg acgaaggatc gatcgaacca actgccagcg
     1141 cacacaaatg ctgtggctcg ttcgaagggg gcctggtgtt gccggggaaa tcgcccccga
     1201 gcgggcccgg tccgaacgct ccggagggca gatagacccg cccggattca aagagcgcga
     1261 cccggtcggt tccccgcgaa aggttccgcc gggcagcgcc gagcaccgag ccgagcagcg
     1321 tggtccgcat caccgactgg tcctcggaga gcggattggc cagcttcacg gcctgggccc
     1381 gcggatcctc cggggccagc ctgagccgat ccttctcacc ggggtcggtg aagctccagc
     1441 cgacgatctc gtcaaagccc gactcacgga gggaatcctc ggcccgccgc tgcagcgcct
     1501 gctggcggct gagcccgccg ggcctgccgg caccggatgg cagggtggca ggcaggttgc
     1561 ggtcgaggtc gctgatccgg ccgacctctt cgaccaggtc gatttcccgg gtcacgtcga
     1621 aataccggtc gggcggtacc gtgaccagca ggtcttcgcc ttcccgctcg accccgaagc
     1681 ccagcatttc aagcgcctcg gcctggcggt cggcttcgat tggcagtccg agaattctct
     1741 ccacccggcc ggcccggagc cggatcggcg cggaagcggg gctcccggaa gcgacgtcaa
     1801 ccacgccggt catcggcacc gcaccgcaga tctcgaccat cagccgggcg gccacggcct
     1861 gggcccgcag ggtcaggtcg ggatgaagct gcttttcgaa gcgcgaggaa gcctccgaac
     1921 ggagcccgag gtcgcgcgag gtcttgagga tgttcgggcc gttccagttg gccgactcca
     1981 tcagcaccgt agtcgtggat tccgacacct cggaaaccga gccgcccatc atgccgccga
     2041 ttccggccag gccgccccgg tcggcgacgg cgaccatctc cgaggtcagt tcgtactcgc
     2101 tgccgtccag cgcggccagc ttctcgccgt tctcggcccg ccggacgatc agctcgccct
     2161 ccgggaccag gtcgaggtcg taggcatgca tcggctggcc ggtcagccac atcacgtagt
     2221 tggtgatgtc gaccacattc gagatcggcc gcatgcccgc agcgatcagc ctcgccttga
     2281 gccaggtcgg tgaggggcca accttcacct cgctgtatcc gcgagcggtg aagcgcgggc
     2341 agaggtcctc gtcctgaacc aggatccgga cccggtcggc ggt
//
LOCUS       DAOQMF010000259         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28120_length_2380_cov_3.304086, whole genome shotgun sequence.
ACCESSION   DAOQMF010000259 DAOQMF010000000
VERSION     DAOQMF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28120_length_2380_cov_3.304086"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLV77_05210"
     CDS             complement(<1..462)
                     /locus_tag="PLV77_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15277.1"
                     /translation="MKRSSVVTFALALATVAALLAPASPASARDEVHRCTNWSWTGPS
                     SWDAVCSKQGISISSPDGRRGIDWGFSNIFCVRANSYRQSAARFIASQRQSINGLRFV
                     RKGRLRQVGRLYFRQTFIVTAGRGRNAGKGQLVFDYAAADNTGTYCYQSSKL"
     gene            complement(496..1122)
                     /locus_tag="PLV77_05215"
     CDS             complement(496..1122)
                     /locus_tag="PLV77_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15278.1"
                     /translation="MREHKRGVEFRWLPILAAALVSLALVIPMLAPTPSGAAYPSSFR
                     RHQEQYWVWYAPRSWIASSGKYDLNISSATGTLWNKYGYSAVVCYNPAAWFKYLRNNY
                     RDTAGAGFGIYSKPLSRTHFTRIGRIKQLPKARYGPAYFRQKLLWTGRRKGSGQVIRG
                     EMIMDIFAIDSFGNCAQRFQTRGAPARGNAASIRLLRLVQSTITQRNL"
     gene            1372..>2380
                     /locus_tag="PLV77_05220"
     CDS             1372..>2380
                     /locus_tag="PLV77_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNC15279.1"
                     /translation="MFERFTERARQVVVLAQEEARTLKHNYIGTEHILLGLLREEEGL
                     AARVLESLEITVERVRAQVVRIVGSGEEVTSGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADSDKIRNEVIRMLSGPGGRRPGQSGSGSSSG
                     SSQGEGSGKKSSKLLDQFGRNLTKLAEDGKLDPVVGRETEIERIMQILSRRTKNNPVL
                     LGEPGVGKTAVVEGLAARIIKGEVPEMLRGKQIYTLDLAALVAGSKYRGEFEERLKKV
                     MKEIASRGDIVLFIDEIHNLVGAGAAEGAIDAASILKPALARGEIQTIGATTLDEYRK
                     YL"
BASE COUNT          455 a          761 c          777 g          387 t
ORIGIN      
        1 gagcttggag ctctggtagc agtaggtgcc ggtgttgtcg gcggcggcgt aatcgaagac
       61 cagctggccc ttgccggcgt tccgtccccg gcccgccgtg acgatgaagg tctggcggaa
      121 gtagagccgg ccaacctggc gcagccggcc cttgcgcacg aagcgaagtc cgttgatgct
      181 ctggcgctgg ctggcgatga atctcgccgc cgactgccgg taactgttcg cgcggacaca
      241 gaagatgttg ctgaagcccc agtcgattcc gcggcgcccg tccggcgagg agatgctgat
      301 gccctgcttg ctgcagaccg cgtcccaact cgacggcccg gtccaggacc agttggtgca
      361 gcggtggacc tcgtcccggg ccgaggcggg ggaggccggc gccagcagcg cggcaacggt
      421 cgcaagggca agggcgaaag tgacgacgga cgagcgcttc atgttcgcaa gcctaacctc
      481 accggcagcg ttgcctcaca ggtttcgctg ggtgatcgtc gactgaacca ggcgcagcag
      541 cctgatgctt gccgcatttc cacgggccgg ggcgccgcgg gtctggaatc gctgggcgca
      601 gtttccgaaa gagtcgatcg cgaagatgtc catgatcatc tcgcccctga tcacctggcc
      661 gctgcccttc cggcggccgg tccagagcag cttctgccgg aaataggcgg ggccgtacct
      721 ggccttcggc aactgcttga tcctgccgat ccgcgtgaaa tgggtgcggc tcagcggctt
      781 cgagtagatg ccgaaaccgg ccccggcggt atcccggtag ttgttgcgca ggtacttgaa
      841 ccaggcggcg gggttgtaac agacgacggc gctgtatccg tatttgttcc agagggtgcc
      901 ggtcgccgag ctgatgttga ggtcgtattt gccggaggaa gcgatccagc tccggggggc
      961 gtaccagacc cagtactgct cctgatggcg ccggaagctg gacgggtagg cggccccgga
     1021 aggtgtgggt gccagcatcg ggatcaccag cgcgagggaa accagggccg cggcaaggat
     1081 cggcagccac cggaactcaa ctccccgctt gtgctccctc atgagccgac cataacgccg
     1141 caagatgccc cgatcaaccc cgcattgccg atccagcccg aggaggcgcc tcgttatact
     1201 ggcttattcg ctcgatcccc caagaccgga ggtcagatcc caaggtgttt cacgactgaa
     1261 acagggatag ggcagccggc actttgccga ctcgcaagac cgaccagaaa aaccgaccga
     1321 ccaatcaccg atactcaggc aaccatcgag gtttgaggta gaagaagaaa cttgttcgag
     1381 agattcacag aacgcgcacg tcaggtagtc gtcctcgctc aggaagaagc gagaacgctc
     1441 aagcacaact acatcggcac cgagcacatc ctgctcgggc tgcttcgcga ggaagaaggt
     1501 ctcgccgcgc gggtcctgga atcacttgaa atcaccgtcg agcgcgtccg ggcacaggtg
     1561 gtccggattg tcggctccgg tgaagaagtc acatccggcc agatcccctt cacgccgcgg
     1621 gcgaagaagg tgctggagct ggccctgcgc gaggcgctca gcctcggcca caactacatc
     1681 ggcaccgagc acatcctgct tggactggtc cgtgagaacg aaggtgtcgc ggcccgcatc
     1741 ctgctcgact tcgacgccga ctcggacaag atccgcaacg aggtcatccg catgctttcc
     1801 ggccccggcg gccgccggcc cggccagagt ggcagcggtt cctcctccgg ctcaagccag
     1861 ggcgagggtt ccggcaagaa gtcctcgaag ctgctcgacc agttcggccg caacctgacc
     1921 aagctggccg aggacggcaa gctggatccg gtcgtcggtc gcgagaccga gatcgagcgg
     1981 atcatgcaga tcctttcccg ccgcaccaag aacaacccgg tcctgctggg tgagcccggg
     2041 gtcggcaaga ccgccgtggt cgagggtctg gccgcccgca tcatcaaggg cgaggtcccg
     2101 gagatgctgc gcggcaagca gatctacacc ctggacctgg ccgcactggt cgccggctcc
     2161 aagtaccgcg gcgagttcga ggaacggctc aagaaagtga tgaaggaaat cgcctcccgg
     2221 ggtgacatcg tcctcttcat cgacgagatc cacaatctgg tcggtgccgg cgccgccgag
     2281 ggcgccattg acgcggcctc gatcctcaag ccggccctgg cccgtggcga gatccagacg
     2341 atcggcgcga ccaccctcga cgagtaccgc aagtacctcg
//
LOCUS       DAOQMF010000260         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28306_length_2371_cov_3.132988, whole genome shotgun sequence.
ACCESSION   DAOQMF010000260 DAOQMF010000000
VERSION     DAOQMF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28306_length_2371_cov_3.132988"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="PLV77_05225"
     CDS             <1..1478
                     /locus_tag="PLV77_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC15280.1"
                     /translation="YLRREREERAAGRSESAALEAAAATSGRAVIVSGITVMIAMAGM
                     YMTGDATFASFATGTIIVVAVAVLGSLTVLPAVLAWLGDRVDKGRIPLIDRLRRSRKI
                     RRVGGEESVLAAGNGGGFWGRFIRTVLRRPGLSAVAAAGLLVLLAVPAFKLHTVVPGA
                     EALPQDLPVIKAYNQMDEAFPGGPEPANVVIEADDVRAPEVQQGIAALRQAVDGPAEF
                     GSPVTTTVSPNGKVEVVNVPLAGSGTDDTSNDALVKLREEVIPETIGKVPGTETNVNG
                     MTAGSKDFNDLMKSRAPLVFAFVLLTAFILLMVAFRSIVIPATSILLNLLSVGAAYGL
                     LVWVFQEGHLEGLLGFESMGGITAWLPMFLFVVLFGLSMDYHVFILSRIREAHDQGLS
                     TSDAIEAGLRRTAGPVTSAAIVMVAVFSIFATLSMLEFKQMGVGLAAAVLIDATIVRG
                     VLVPASMKLLGKWNWYLPAWLEWLPKLNLEGTPEPAPRPAV"
     gene            1543..2151
                     /locus_tag="PLV77_05230"
     CDS             1543..2151
                     /locus_tag="PLV77_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNC15281.1"
                     /translation="MTDAEALKIDEQATADPARGPGQAAVVDPAEVAGQEVIDAASPE
                     AKRVALQLGTLFRRMLSYGAATSALLDAAALGLSFLEFKAIMSLGVFEPADSFCLADI
                     GESTGASMPAVSRAIDSLVRKGLVSRVEDPHDRRRRLIALTDEGHRVTNAALMGRAVG
                     AVRLADSFSEDELKALDSLLSKLIEREDLASIHRQLEGAVQQ"
     gene            2148..>2371
                     /locus_tag="PLV77_05235"
     CDS             2148..>2371
                     /locus_tag="PLV77_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC15282.1"
                     /translation="MSKYQHLITDENRKWWTLGAMCLALFMVMLDNTVVNVALPSIQR
                     DLDTSISGLEWIVNGYTLSFAVLLAVGGRIG"
BASE COUNT          378 a          783 c          797 g          413 t
ORIGIN      
        1 tctacctgcg acgcgagcgg gaggaacggg cggccggacg gtccgaatcg gccgcgctgg
       61 aggctgccgc ggcaacttcc ggccgggccg tgatcgtctc gggaatcacg gtgatgatcg
      121 cgatggccgg catgtacatg accggagacg ccaccttcgc gtcattcgcc accggcacga
      181 tcatcgtggt cgcggtcgcc gtgctcggtt cactgaccgt gctgccggcc gtgctcgcct
      241 ggctcggcga ccgggttgac aagggccgga tcccgctgat cgaccggctg cgacggagcc
      301 ggaagatccg gcgggtcggg ggcgaggaga gcgtgctggc tgccggcaac ggcggcggtt
      361 tctggggtcg attcatccgg accgtgctgc ggcgccccgg cctctcggct gtcgccgccg
      421 ccggcctgct cgtgttgctg gcggtcccgg ccttcaaact ccacaccgtc gtgccggggg
      481 ccgaagccct gcctcaggac ctgccggtga tcaaggccta caaccagatg gacgaagcct
      541 tccccggcgg cccggagccg gccaacgtgg tgatcgaggc cgatgacgtg cgtgcgcccg
      601 aggttcagca gggcatcgcc gcgctcaggc aggcggtcga cggtccggcc gagttcggct
      661 caccggtcac cacgaccgtg agcccgaacg gcaaggtcga ggtggtgaac gtgcccctgg
      721 ccggcagcgg caccgatgac acatccaatg acgcgctggt caagctccgc gaggaggtga
      781 tcccggagac gatcggcaag gtgccgggca ccgaaaccaa cgtcaatggc atgacggccg
      841 gttcgaagga tttcaacgac ctgatgaaga gccgggcgcc gctggtcttc gccttcgtct
      901 tgctgaccgc cttcatcctg ctgatggtcg cgttccgttc gatcgtgatc ccggcgacct
      961 cgatcctgct caatctgctc tcggtcggcg ccgcctacgg attgctcgtc tgggtcttcc
     1021 aggaagggca tctcgagggt ctgctcggtt tcgagtcgat gggcgggatc accgcctggc
     1081 tgccgatgtt cctcttcgtg gtcctgttcg gactctcgat ggactaccac gtgttcatcc
     1141 tcagccggat ccgggaagcg cacgaccagg ggttgagcac ttcggacgca atcgaggccg
     1201 gcctccggcg cactgccggg ccggtcacct ccgccgcgat cgtgatggtg gccgtgttct
     1261 cgatcttcgc caccctctcg atgctggagt tcaagcagat gggggtcggg ctggcggcgg
     1321 ccgtcctgat cgacgcgacg atcgtgcggg gtgtgctcgt cccggcgagc atgaaactgc
     1381 ttggcaagtg gaactggtac ctgccggcct ggctcgagtg gctgccgaag ctcaacctcg
     1441 aaggcacccc cgaaccggcc ccccggccag ccgtgtgaag tgcatcacat aagcaactat
     1501 tgcgtttgac aaatagttgc gaaagagcaa gtatcttcag tcatgaccga tgcggaagcg
     1561 ctgaagatcg acgagcaggc gactgccgat ccggcccggg gcccgggcca ggcggcagtc
     1621 gtcgacccgg ccgaagttgc cgggcaggaa gtgatcgacg cggcatcgcc cgaggcgaag
     1681 cgggtcgccc tgcagctcgg gaccctgttc cggcggatgc tctcttacgg ggcggcgacc
     1741 agcgccctgc tcgacgcggc tgcgctcggt ctctcatttc tcgagttcaa ggcgatcatg
     1801 agtctcgggg tcttcgaacc cgccgacagc ttctgcctcg ctgacatcgg cgagtcaacc
     1861 ggggcctcga tgcccgccgt gagccgggcg atcgacagcc tggtccgcaa gggcctcgtt
     1921 tcccgggtcg aggatcccca cgaccggcgc cgccggctga tcgcgctcac cgacgaaggc
     1981 caccgggtca ccaacgccgc cctgatgggc cgtgcggtcg gcgcggtgag actggccgac
     2041 agtttcagcg aggacgaact caaagccctc gactccctgc tctcgaagct gatcgagcgg
     2101 gaagaccttg cctcaattca ccggcagttg gaaggagcag ttcagcagtg agcaagtacc
     2161 agcacctgat caccgacgag aaccgcaagt ggtggacgct gggcgcgatg tgcctggccc
     2221 tgttcatggt gatgctcgac aacaccgtcg tcaacgtcgc cctcccctcg attcagcgcg
     2281 acctcgacac ctcgatctcc ggcctcgaat ggatcgtcaa cggctacacg ctctctttcg
     2341 cggtgctgct ggcggtcggc ggccggatcg g
//
LOCUS       DAOQMF010000261         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28323_length_2370_cov_3.898056, whole genome shotgun sequence.
ACCESSION   DAOQMF010000261 DAOQMF010000000
VERSION     DAOQMF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28323_length_2370_cov_3.898056"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLV77_05240"
     CDS             complement(<1..617)
                     /locus_tag="PLV77_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC15283.1"
                     /translation="MAKKKKGKSVAKPPNLLLIITDQQRYPMHWPEDQEWLDELMPAD
                     AELARTGVTFEETCIASCMCSPSRASLFTGRWPAEHGVDLTLTRGGAEIDRSHSKHAL
                     RKGIEAVRHGEMSALEMVTNTVKGAARRANGGTDERELDPNMENLPSVLKRAGYKTVL
                     KGKWHLTQPVGSDWSQADAERLADEYGFEGWVPPDAGENIEPSHFG"
     gene            complement(620..1966)
                     /locus_tag="PLV77_05245"
     CDS             complement(620..1966)
                     /locus_tag="PLV77_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15284.1"
                     /translation="MKKVFLAAIALLLAGTAIAIPARAALVENGYNPLDLPTAGCFWT
                     GPFTAANEKTNTAYPGTEITYWGAKFRTPAGAVLRLRGQYPHARYSSLNAYESSGGVP
                     TGSISDRQIKPDRGSVNTSLPGKDRKAKKRAWTVRVLGSAAPEKPVANTLYAQPSSED
                     YQDIIYRVYVADKGRSKSGGVPIPTPELTLEDGTVLTGKALCAALNSNHDYKSQSVPT
                     VLYDALVNWPGKDPLTNPAEADFSFIKFFNLQLSLARYKTQAEFDAAWEANPNEVGTL
                     YGNNDARYMTGAFSTRFGKVLVIKGSMPTTPQTYRGQRRAKAGQLVEWDMCTLESVAT
                     TKTNKCLFDEQVPLRGKQRRYTIVVAKAGDRPKNATRECGVAFLPADPDGDGVGRKDA
                     GLLLTRNVIPASNFKHSIQNVTTPYNAARVMGRYYPRGTYTSKAAFQKLGCPKAGN"
     gene            complement(2017..>2370)
                     /locus_tag="PLV77_05250"
     CDS             complement(2017..>2370)
                     /locus_tag="PLV77_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15285.1"
                     /translation="GDAFLEAIAQMRECYMPKAGFGPRFPLKVEETGWPTGPGRSEEE
                     QAQVLRQFVDTAHRYRGTYKVTDFRWFGLRDNNSDGPDYQSYFGLLRSNYSKKPAFYE
                     YLTRVSKLGAGRSSR"
BASE COUNT          345 a          754 c          796 g          475 t
ORIGIN      
        1 ccgaagtggc tcggctcgat gttctcgccg gcatccggcg gcacccagcc ctcgaagccg
       61 tattcgtcgg cgaggcgctc ggcgtcagcc tgggaccagt cgctgccgac cggctgggtc
      121 agatgccact tgcccttgag cacggtcttg tagccggccc gcttgagcac gctgggcagg
      181 ttttccatgt tcgggtcgag ctcgcgctcg tcggtgccgc cgttggcccg gcgggctgcc
      241 cccttgacgg tgttggtcac catctccagg gccgacatct cgccatgcct gaccgcttcg
      301 atgcccttgc gcagggcgtg cttgctgtgc gagcggtcga tctccgcccc gccccgggtc
      361 agggtcaggt ccaccccgtg ctcggccggc cagcgccccg tgaagagcga ggcacggctc
      421 ggggagcaca tgcaggaggc gatacaggtc tcctcgaagg tgactccggt gcgagccagt
      481 tcggcgtcgg ccggcatcag ctcgtcgagc cactcctgat cctccggcca atgcatcgga
      541 tagcgctgct ggtcggtgat gatcagcagc aggttgggcg gtttcgccac gctcttcccc
      601 ttctttttct ttgccacgct cagttacccg ctttcggaca gcccagcttc tggaaagcgg
      661 ccttgctggt gtaggtgccc cgcgggtagt aacggcccat gaccctggcc gcgttgtagg
      721 gcgtggtcac gttctggatc gaatgcttga agttcgaggc cgggatcacg tttctggtca
      781 ggagcagccc ggcgtccttg cggccgacac cgtccccgtc agggtcggcc ggcaggaagg
      841 ccacgccgca ctcgcgggtt gcgttcttcg gccggtcgcc cgccttggcg acgacgatcg
      901 tgtagcggcg ctgtttgccc cggagcggca cctgctcatc gaagaggcat ttgttggtct
      961 tcgtggtcgc caccgactcg agcgtgcaca tgtcccattc aaccagctgc ccggccttcg
     1021 cccggcgctg cccccggtag gtctgcggtg tcgtcggcat actgcccttg atcaccagca
     1081 ccttgccgaa gcgggtgctg aaggcaccgg tcatgtagcg ggcgtcattg ttgccgtaaa
     1141 gcgtgccgac ctcgttcggg ttggcctccc atgcggcgtc gaattcagcc tgggtcttgt
     1201 agcgggccag cgagagctgg aggttgaaga acttgatgaa gctgaagtcc gcctcggccg
     1261 ggttggtcag cggatccttg ccgggccagt tgaccagggc gtcatagaga acggtcggaa
     1321 cgctctgcga cttgtaatcg tgattcgagt tgagcgccgc gcagagcgcc ttgccggtca
     1381 gcacggttcc gtcctcaagc gtcagttccg gggtcgggat cggaacgccg ccggacttgc
     1441 tccggccctt gtccgcgacg tagacccggt agatgatgtc ctgatagtcc tcggacgagg
     1501 gctgggcgta gagggtgttg gcgaccggct tttcgggagc ggccgagccg agcacgcgga
     1561 ccgtccaggc ccgctttttg gccttgcggt ccttgccggg aaggctggtg ttgaccgagc
     1621 cccggtccgg tttgatctga cgatcggaaa tcgaaccggt cggcacgcca ccgctcgatt
     1681 cgtaggcgtt gagcgacgag tatcgggcat gcgggtactg gccccgcagc ctcagaaccg
     1741 cgcccgccgg ggtgcggaat ttcgcacccc agtaggtgat ctcggttccg ggataggcgg
     1801 tgttggtctt ctcgttcgcg gcggtgaagg gaccggtcca gaagcagccg gcggtcggca
     1861 ggtcgagcgg gttgtagcca ttctcgacca gggcggcccg ggccgggatc gcaatcgcgg
     1921 ttccggcaag aagcaaagcg atcgctgcca gaaaaacctt cttcactgcc gtcatgcctc
     1981 tgctcctgct gtcgaattgg tcgccggtcc ccgccctcag cgggaagacc tgcccgcgcc
     2041 cagtttactg acgcgcgtca agtattcata gaacgcgggc tttttcgaat agttgctgcg
     2101 aagcaggccg aaataggact gatagtcggg accgtcgctg ttgttgtccc ggagcccgaa
     2161 ccagcggaaa tcggtgacct tgtaggtgcc gcggtaacgg tgcgccgtgt ccacgaactg
     2221 gcgaaggacc tgcgcctgct cttcctcgct tcgccccggc ccggtcggcc atccggtttc
     2281 ctcgaccttc agggggaagc ggggcccgaa gccggccttg ggcatgtagc actcgcgcat
     2341 ctgggcgatt gcctcgagaa aggcgtctcc
//
LOCUS       DAOQMF010000262         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28448_length_2365_cov_5.216450, whole genome shotgun sequence.
ACCESSION   DAOQMF010000262 DAOQMF010000000
VERSION     DAOQMF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28448_length_2365_cov_5.216450"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLV77_05255"
     CDS             <1..735
                     /locus_tag="PLV77_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNC15286.1"
                     /translation="EMGVNDRNKGKILRRIKSDPWYRKRFPKAFRWSKKPVNWFNIVR
                     SISAFQRSIITGTSKYNRFLRGKAELSGSERSGLNLFMGEEGECHHCHGSFIFDDQAT
                     YAGGPLEKLKFHNTGLYNIGGTGAYPTGNRGLYEITGKKKDMGAFRAPSLLNVAKTAP
                     YMHDGSVKTLMDVVDIYARGGRNITEGPFAGDGATNPHKDPLISGINLSEQDKKDIVA
                     FLKTLSERDYSKDPRFWDPFKHKSRK"
     gene            738..1103
                     /locus_tag="PLV77_05260"
     CDS             738..1103
                     /locus_tag="PLV77_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNC15287.1"
                     /translation="MDSITSSNTVRGLVAGTIVAAAMILGGPAGQAEAHTGVTSTSAN
                     SRMLRVFFAAPIRRGTLRARNASGAIVSKGRGGRDPRNINALRVGLRNLRGGYYRATW
                     SIVGLDGHHQSGTVRFRVR"
     gene            1108..1752
                     /locus_tag="PLV77_05265"
     CDS             1108..1752
                     /locus_tag="PLV77_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HNC15288.1"
                     /translation="MSRRVSSFIACALIGGSALVLAVPAAAHIQVEPTEVAPGDAALF
                     TLLVPGESAAGTSEVKLKVPPGVYPFSYEETPGWKRKLVKKPNGMVDQVIWTGTAAPD
                     GLVRFSFLAGTPEKLGEIKWAAIQNYANGEESRWIGMPESENPAPVTIVSDSVPRQNA
                     GGESEVAHEHASPTSSENATTDSETDWPITIAALLGFFFGLSSLVILLVNRINS"
     gene            complement(1749..>2365)
                     /locus_tag="PLV77_05270"
     CDS             complement(1749..>2365)
                     /locus_tag="PLV77_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15289.1"
                     /translation="ALADWPKQNRLVEIFDNRDGNLSIFGTVIDHAAGVDAPASGTSA
                     ANMSTDELASISRVVGYNENQSGAEACGSEKCGEGEAEDRNVELVIKDPRKPKAIVKK
                     VTVAPKKRNLKAGRKFNLVVKVTNFITATADAKNVKVTVKSSNKRVKVKSAVIRKISP
                     GKTATVKIPVRALGKARGKSKITVTAAGNKAYSNIKVIPKKKRR"
BASE COUNT          471 a          731 c          711 g          452 t
ORIGIN      
        1 gaaatgggcg taaacgaccg gaacaagggc aagatccttc gaaggatcaa gagtgatccg
       61 tggtaccgaa agcgcttccc gaaggcattc cgctggtcga agaaacctgt caactggttc
      121 aacatcgtcc gctcgatcag cgcattccag cgcagcatca tcaccgggac ctcgaaatac
      181 aaccggttcc tgcgcggcaa agccgaactc agcggttcgg aacgaagcgg cctcaatctc
      241 ttcatgggcg aagagggtga atgtcaccac tgtcacggca gcttcatctt cgatgaccag
      301 gcgacctacg cgggcgggcc gctcgagaag ctcaagttcc acaacaccgg cctctacaac
      361 atcggtggaa ccggcgccta ccccaccggc aaccgcggcc tctacgaaat caccggcaag
      421 aagaaggaca tgggtgcttt cagggcaccg tcgctcctca acgtggcgaa aacggcccct
      481 tacatgcatg acggctcggt caagacgctc atggatgtgg tcgacatcta tgcccgcggt
      541 ggccgcaaca tcaccgaagg cccattcgcc ggtgacgggg cgacgaatcc tcacaaggac
      601 ccgttgatct ccggaatcaa cctcagtgaa caggacaaga aggacatcgt cgccttcctc
      661 aagacgttga gcgaacgcga ttactccaag gacccccgct tctgggatcc cttcaagcac
      721 aaatcaagga aatgaacatg gacagcatca ccagcagcaa cacggtccgc ggtctggtcg
      781 ccggcacaat cgtcgcggca gcaatgatcc tcggtgggcc ggccggccag gcggaggccc
      841 acaccggtgt cacatcgacc tccgccaaca gcaggatgct ccgggttttc ttcgctgccc
      901 cgatcaggag gggaaccctg agagcccgaa acgcgagcgg ggcgatcgtg tccaaaggcc
      961 gcggaggacg tgacccacgc aacatcaacg cgctgagggt gggactgcgg aatctgcgtg
     1021 gcggctatta cagggcgacc tggagcatcg tcgggctcga cgggcaccac cagtcgggaa
     1081 ccgtgcgctt ccgggtgagg tagccccttg tcaaggcggg tcagcagttt cattgcctgc
     1141 gccctgatcg gcggatcagc cctggtccta gccgttcccg cggcagcaca cattcaggtt
     1201 gaacccacgg aggttgctcc gggagacgcg gcactcttca ccctgctggt tcccggcgaa
     1261 tctgccgctg gaacttccga agtcaagttg aaggtccctc cgggcgttta cccgttttcc
     1321 tacgaggaga ctcccgggtg gaagcggaaa ctcgtcaaga aaccgaacgg gatggtggac
     1381 caggtgatct ggaccggaac ggcagccccg gacgggctgg tgcgtttcag cttcctggcc
     1441 ggaacgccgg agaagctggg tgagatcaaa tgggcggcaa ttcagaacta cgcgaacggg
     1501 gaggagtcac gatggatcgg aatgccggaa tcggaaaatc ccgccccggt cacgatcgtt
     1561 tccgattcag ttccccgtca aaacgccggc ggcgaaagcg aggtcgctca cgaacacgct
     1621 tcgccgacca gctccgaaaa cgcgaccacg gattcggaga cggactggcc tatcaccatt
     1681 gccgcattgc tcgggttctt cttcgggctc tcgtcgctgg tgatcctgct ggtcaacaga
     1741 ataaactcct agcgacgctt ctttttgggg atcaccttga tgttcgagta agccttgttg
     1801 ccggcggcgg ttaccgtgat ctttgacttt ccgcgggcct tgccgagggc gcggaccggg
     1861 atcttcacgg tggcggtttt gccagggctg atcttcctga tcaccgcgga cttcaccttg
     1921 acccgcttgt tcgatgactt gaccgtcacc ttgacgttct tcgcgtccgc ggtggcggtg
     1981 atgaagttgg tcaccttgac caccaggttg aacttgcgcc ctgctttcag gttccgcttc
     2041 ttcggggcca cggtgacctt ttttacgatc gccttcggct tgcgcgggtc cttgatcacc
     2101 agttcgacgt tgcggtcctc ggcttcgcct tcgccgcact tctccgaacc gcaggcttcc
     2161 gcgcccgact ggttctcgtt gtaaccgacc acccgggaaa tcgaggcgag ttcgtcggtg
     2221 ctcatgttgg cggcgctggt accgctcgcc ggtgcgtcaa cgccggcggc gtgatcgatc
     2281 acggtgccga agatcgagag gttgccgtca cggttgtcga agatctcgac cagacggttc
     2341 tgcttcggcc agtcggccag ggccg
//
LOCUS       DAOQMF010000263         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28542_length_2361_cov_3.011275, whole genome shotgun sequence.
ACCESSION   DAOQMF010000263 DAOQMF010000000
VERSION     DAOQMF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28542_length_2361_cov_3.011275"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLV77_05275"
     CDS             complement(<1..966)
                     /locus_tag="PLV77_05275"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNC15290.1"
                     /translation="MSETTQVTAPTKNARLLAWVDEIAELTQPDQVYWCDGSAEEYNA
                     LAGRLVEAGTFEKLSDAKRPNSYLALSDPGDVARVEDRTFICSEKEEDAGPTNNWRDP
                     EEMRATLKPFFEGSMKGRTMYVVPFSMGPLGSNIAHIGVQLTDSAYVAASMRIMTRMG
                     KGALDVLGDGEFVPCVHSVGMPLEEGQEDVPWPCNAENKYIVHYPETREIWSYGSGYG
                     GNALLGKKCFALRIASVMARDEGWMAEHMLILKLTSPEGEVKYVTGAFPSACGKTNLA
                     MLIPTMEGWTVETVGDDIAWMKFDDEGRLRAVNPEAGFFGVAPGTG"
     gene            complement(1148..1447)
                     /locus_tag="PLV77_05280"
     CDS             complement(1148..1447)
                     /locus_tag="PLV77_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HNC15291.1"
                     /translation="MLVRPLFLLAGPFLAAMILAGCGAGDVIDDQKTEIAVRFDVQDA
                     TGEPVRSVTCPSGVPVSVGTRFTCHVVAKSGDDAVAELEVTSDDGDLRMLSLTAP"
     gene            1544..>2361
                     /locus_tag="PLV77_05285"
     CDS             1544..>2361
                     /locus_tag="PLV77_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNC15292.1"
                     /translation="MIFYEYEARKIVELAGIPVTKYGFCQTPEEARKIAEEIGGPTVI
                     KSQVLTGGRMKAGGVKFADTPDEAAQHAEDILKLEINGHMPVGVLVDPKAEVKQEYYA
                     SVVWDGTAKKPLMLFSDMGGIDIEQVAEEHPDHVGRGHISNLLPVGDYEAKQVIAATG
                     VTGSQLNRATPILAKLARLFRENDMILAEINPLAELTDGTFVALDAHMEMESEAEGRQ
                     KKLLRKELGIAEDDNRDPYVPSEFEKNVKEIDAADHRGVIQGKDHGFEGNLGLV"
BASE COUNT          460 a          757 c          757 g          387 t
ORIGIN      
        1 accggtgccg ggggcgacgc cgaagaagcc agcctcggga ttgacggcgc gcaggcggcc
       61 ctcgtcatcg aacttcatcc aggcgatgtc gtcgccgacc gtctccacgg tccagccctc
      121 catcgtcgga atcagcatcg cgagattggt cttgccgcag gccgagggga aggcaccggt
      181 cacgtacttg acctcgcctt cggggctggt cagcttgaga atcagcatgt gctcggccat
      241 ccagccctcg tcgcgggcca tcaccgaggc gatccgcagc gcgaaacact tcttgccgag
      301 cagcgcgttg ccgccgtagc cggaaccgta tgaccagatc tcccgggtct ccgggtagtg
      361 gacgatgtac ttgttctcgg cgttgcaggg ccacggcacg tcttcctggc cttcctcgag
      421 cggcatgccg accgagtgga cgcagggaac gaactcgcca tcaccaagca cgtcgagcgc
      481 gcccttgccc atccgggtca tgatccgcat cgacgcggcg acgtaggccg agtcggtgag
      541 ctgaacaccg atgtgggcga tgttcgagcc gagcgggccc atcgagaacg gcaccacgta
      601 catggtgcgg cccttcatcg agccctcgaa gaacggcttc agggtggccc gcatctcctc
      661 ggggtcgcgc cagttgttgg tcggcccggc gtcttcttcc ttctccgaac aaatgaaggt
      721 tcggtcctcg acccgggcga cgtcaccggg atccgaaagg gcgaggtacg agttggggcg
      781 ctttgcgtca gagagctttt cgaaggtccc ggcttccacc aggcgcccgg cgagggcgtt
      841 gtactcctcg gcggaaccgt cacaccagta gacctgatcg ggctgggtca gctcggcgat
      901 ctcatccacc caggcaagca gcctggcgtt cttggtcggg gcggtcacct gggtcgtttc
      961 actcactggc gctcttcaac tccttggtca acgcgcctgc gaaggagagg gcggcgcgga
     1021 tttagccggt tacggaattg tgacggagat cagtgcgaat tgcggtctga ccagcgaggg
     1081 tctgcggata actaccgaac atgattcaca gacgactccg ccggttacca ccactggttt
     1141 ggccgaatca gggggcggtg agcgacagca tccgcaaatc gccgtcatcg gaggtgactt
     1201 cgagttcggc cacggcgtca tcgcccgact tcgccaccac gtgacaggtg aagcgggtgc
     1261 cgaccgagac cggcaccccg gacgggcagg tcaccgagcg caccggctcc ccggtggcgt
     1321 cctgcacgtc gaaccggacc gcgatctcgg tcttctgatc gtcgatcacg tcaccggctc
     1381 cgcagccggc aaggatcatg gcggccagaa acgggccggc gagcaggaag agaggacgga
     1441 ccaacacggg ccgcacacta catagcgtcc ggtcgcgccg ccgattccta gcgactcaat
     1501 cggaataacc ggtcgcccgg ttctggaata gattttcgcc gcaatgatct tctacgaata
     1561 cgaggcaaga aagatcgtcg agctggccgg aattccggtc accaagtacg gcttctgcca
     1621 aacccccgaa gaggcacgga agatcgccga ggaaattggc ggtccgaccg tgatcaagtc
     1681 ccaggtcctg accggtggcc gcatgaaagc gggcggcgta aagttcgccg acaccccgga
     1741 tgaggccgcg cagcacgccg aggacattct caagctcgag atcaacggcc acatgccggt
     1801 cggcgtcctg gtcgacccga aggccgaggt caagcaggag tactacgcct cggtggtctg
     1861 ggacggcact gccaagaagc cgctgatgct gttcagcgac atgggcggca tcgacatcga
     1921 gcaggtcgcc gaggagcacc cggaccacgt gggccgcggt cacatctcga acctgcttcc
     1981 ggtcggtgac tacgaggcca agcaggtcat cgctgcaacc ggcgtgaccg gcagccagtt
     2041 gaaccgggca acgccgatcc tcgccaagct ggcccgcctc ttccgtgaaa acgacatgat
     2101 cctggccgag atcaatcccc tggccgagct gaccgacggg accttcgtcg cactcgacgc
     2161 ccacatggag atggagtccg aggccgaggg tcgtcagaag aagctcctgc ggaaggaact
     2221 cgggatcgcc gaggacgaca accgcgaccc gtatgtcccc tccgagttcg agaagaacgt
     2281 caaggagatc gacgcggccg atcaccgtgg cgtcatccag ggcaaggacc acggattcga
     2341 gggcaacctc gggctggtca t
//
LOCUS       DAOQMF010000264         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28605_length_2358_cov_3.645680, whole genome shotgun sequence.
ACCESSION   DAOQMF010000264 DAOQMF010000000
VERSION     DAOQMF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28605_length_2358_cov_3.645680"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLV77_05290"
     CDS             complement(<1..844)
                     /locus_tag="PLV77_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNC15293.1"
                     /translation="MKTATVGVWAVIGGMLIGMWAGGHPDNLPDSLREIFVADEVQQS
                     VSDQAAELIHDNYFRATDPDQVQDSSINGMVRQIRRQYHDRFSHYFTPKQNEQLSESL
                     QGSFSGVGMTVGANAKKGLEVGYVFRKSPADEAGLKAGDVIVEVNGESIRGEDVDLIV
                     AKIKGPEGTDVTLGVRKAGKGPVRDFKLTREEIRVPITANRIRKVDGKSLGYVQLTTF
                     SQGAGLALRRAVERVIGKGAEGIVIDLRDNGGGLLEEAVFTSSVFLDEGDVVCTRGNP
                     EVRGI"
     gene            complement(894..1808)
                     /locus_tag="PLV77_05295"
     CDS             complement(894..1808)
                     /locus_tag="PLV77_05295"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNC15294.1"
                     /translation="MGKIFFFTGEALRAIKRNVAPTTAAVVTTVLTALLLGVLIPVFQ
                     TAQSKGEDVRGQLELRTFLYDDATKAETQALMVKLEGLPHVESVTLLTKADAKKEFLA
                     SDFGKQNPDALKQLGGKNPLPANFVIKPDDASNLEGIRNAIQPVGAKGDPQYISPIIA
                     SVDDRQEDARDIEQVTGALKLILTVITVLLICASLLLIGNTIRLSIYTRRREIEVMRL
                     VGATRWFIRWPFMIEGVVVGILGGAIAILVLWIGKVTVVDPLSDKFALMNAQDSMSFA
                     ALVGCLFIAAILVSAIGSGLTMRRFLKV"
     gene            complement(1808..>2358)
                     /locus_tag="PLV77_05300"
     CDS             complement(1808..>2358)
                     /locus_tag="PLV77_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC15295.1"
                     /translation="RNIGTVFQDFKLLPSRTVYDNVAYALQVIGASRAEIRQKVPETL
                     RLVGLSTKLHNYPDELSGGEQQRVSVARAFVNHPPLLLADEPSGNLDPVTSVGIMQLL
                     YRINKTGTTVVVVTHDREMVDRMRRRVVELYEGRVIRDEVAGGYTEESTTEFGVRMRA
                     EMGVGVEGHTNGNSGNSGKGAH"
BASE COUNT          398 a          748 c          775 g          437 t
ORIGIN      
        1 caattccgcg cacctccgga ttccctcggg tgcagacgac gtcgccttca tcgaggaaga
       61 cgctggaggt gaagaccgcc tcctcgagca ggccgccgcc gttgtcgcgg aggtcgatga
      121 cgatcccctc ggcccccttc ccgatcaccc gctcgaccgc cctccggagg gccagcccgg
      181 caccctggct gaaggtcgtc agctggacgt agccgaggga cttcccgtcc accttgcgga
      241 tccggttggc ggtgatcggt acccggattt cctcgcgggt gagtttgaag tcccggaccg
      301 ggcccttgcc cgccttgcgg accccgaggg tcacgtcggt cccctccggc cccttgatct
      361 tcgccacgat caggtcgacg tcttcacccc ggatcgactc gccgttgact tccacgatca
      421 catcaccggc tttcaggccg gcctcgtcag ccggggactt gcggaagacg taaccgactt
      481 cgagcccctt tttcgcattg gcaccgacgg tcatcccgac gccgctgaag ctgccctgaa
      541 gcgactcgct gagctgctcg ttctgcttcg gggtgaagta gtgggagaag cggtcgtggt
      601 actggcgccg gatctgcctg accatgccgt tgatcgacga gtcctggacc tgatccggat
      661 cggtcgcccg gaaatagttg tcgtggatca gttcggcagc ctggtcggaa accgattgct
      721 ggacctcgtc ggcgacgaag atctcgcgca gcgaatcagg caggttgtcc ggatggccac
      781 cggcccacat gccgatgagc atgccgccga tgacggccca gacgccaacg gttgcggtct
      841 tcaactggcc gccggctgac tctgaatgac gcccggcagc gggcccggcc ggctcagacc
      901 ttcaggaatc tgcgcatggt cagtccggac ccgatcgccg aaaccaggat cgcggcgatg
      961 aacaggcagc cgacgagggc cgcaaaggac atcgagtcct gtgcgttcat cagggcgaac
     1021 ttgtccgaga gggggtcgac gactgtgacc ttgccaatcc acagcaccag tatcgcaatc
     1081 gcgccgccga ggatgccgac cacgacccct tcgatcatga agggccagcg gatgaaccag
     1141 cgggtcgcac cgaccaggcg catgacctcg atctcgcgcc ggcgggtgta gatggaaagc
     1201 cggatcgtgt tgccaatcag gagcagcgag gcacagatca gcagcacggt gatcacggtg
     1261 aggatcagtt tcagggcgcc ggtgacctgt tcgatgtcgc gggcgtcttc ctggcggtca
     1321 tcgaccgagg cgatgatcgg cgagatgtac tgggggtcac ccttggcgcc gaccggctgg
     1381 atcgcgttgc ggatcccttc caggttggaa gcgtcatcgg gcttgatcac gaagttggcc
     1441 ggcagcgggt tcttgccacc gagctgcttc agggcgtcgg ggttctgctt gccgaagtca
     1501 ctggccagga attccttctt ggcgtcggcc ttggtcagca gggttaccga ctccacgtgt
     1561 ggcagccctt ccagcttcac catcagggcc tgggtctcgg ccttggtcgc gtcgtcgtaa
     1621 aggaaggtgc gcagctcgag ctggccacgc acgtcctcgc ccttgctctg ggcggtctgg
     1681 aagacgggga tcagcacacc gagcagaagc gcggtgagca cggtggtcac taccgcagcc
     1741 gtggtcggcg cgacgttccg cttgatcgcg cggagcgctt cgccggtgaa aaagaagatc
     1801 ttgcccatca gtgtgctccc ttgcccgaat ttccagagtt gccgttggta tggccttcga
     1861 cgccaacgcc catctcggcc cgcatgcgga caccgaattc ggtggtcgac tcctcggtgt
     1921 aaccgccagc gacctcgtcg cggatgaccc ggccttcgta gagctcgacc acgcggcggc
     1981 gcatgcggtc gaccatttcc cggtcgtggg tgaccacgac gacggtggtt ccggtcttgt
     2041 tgatccggta gagcagctgc atgatgccga ccgaggtgac cgggtcgagg ttgccgctcg
     2101 gctcatcggc cagaagcaga ggcggatggt tgacgaaggc ccgggcgacc gaaacgcgct
     2161 gctgctcgcc gccggaaagc tcgtcggggt agttgtgaag cttggtcgag agaccgacca
     2221 ggcggagggt ctccggcacc ttctgccgga tttcggccct tgaagcaccg atcacctgaa
     2281 gcgcgtaggc gacgttgtcg taaacggtgc ggctcggcag cagcttgaag tcctggaaga
     2341 cggtgccgat gttgcggc
//
LOCUS       DAOQMF010000265         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28635_length_2356_cov_4.417210, whole genome shotgun sequence.
ACCESSION   DAOQMF010000265 DAOQMF010000000
VERSION     DAOQMF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28635_length_2356_cov_4.417210"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLV77_05305"
     CDS             <1..315
                     /locus_tag="PLV77_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNC15296.1"
                     /translation="QFDLIMTPTMATVPPKLGHFDQNGPDPMDAMLKAREIATFTGIF
                     NATGQPAISVPMEMSEEGLPIGIQFVAPMWGEHVLIQLAGELERAHPWIGRRAPGVLD
                     AG"
     gene            326..1195
                     /gene="dapA"
                     /locus_tag="PLV77_05310"
     CDS             326..1195
                     /gene="dapA"
                     /locus_tag="PLV77_05310"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNC15297.1"
                     /translation="MSEIRGVITALATAFRPDGELDIDSTRKLAAYLVENGSHGLVVA
                     GSTGESSTLEDDEKIDLTRAVVEEVGPEVTVIVGSGSNDTRHSVELTKMAAEAGADAA
                     LIVTPYYVRPNRAGIRRHFEAVAEGSPGFPIVAYNIPSRTGINMPPEFLAELAQIDEV
                     KAVKQANDDDLGPIEGLDVLAGNDGTFLPTLKFGGAGGILVASHLDNGRMRTIWDLWQ
                     EGRHDEAEALDNQLQPLYEAMGVVVNPIPVKAALAARGLLPPTMRLPLVEPTDQERDA
                     VEELLAEAGVPAA"
     gene            1192..>2356
                     /locus_tag="PLV77_05315"
     CDS             1192..>2356
                     /locus_tag="PLV77_05315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNC15298.1"
                     /translation="MSQDRIKLLPLGGLGEIGKNMMVIEYRGSIVVVDTGLMFPTPEM
                     LGIDLVLPDFEYLRDRADDIEAIILTHGHEDHVGALPYALREIGFPKVIYGGMLTVGM
                     VRSKLDEHKLGSAPLTELPAGQKVSAGPFEIELVHLSHSIPDMRGVIIGTDLGKIFMT
                     GDYKFDQTPVDGRPADVARLAALGDEGLLLLCGDSTNADRPGVSPSESSVGPALYDTF
                     SRCKGRIIVTSFASNIHRVQQVIDAAERLDRKVALVGRSMRKNFNIASNLGMANAPEG
                     LLIQPREIEDFPDERVVVISTGSQGEPFSALRRMANNDHRDVELHSGDTVVFSATPVP
                     GNERAVNETIDRIYELGATVITPKDAPIHASGHGYREEIKMMLNLTRPKYVMPV"
BASE COUNT          432 a          790 c          741 g          393 t
ORIGIN      
        1 cagttcgacc tgatcatgac cccgaccatg gccacggtgc cgcccaagct cggtcatttc
       61 gatcagaacg gacccgaccc gatggatgcg atgctgaagg cccgtgagat cgccaccttc
      121 accggcatct tcaacgcgac cggccagccg gcgatttcgg tgccgatgga gatgagcgag
      181 gaaggtctgc cgatcgggat ccagttcgtg gcgccgatgt ggggcgagca cgtgctgatc
      241 cagctcgccg gcgagctcga gcgggcccat ccctggatcg gccgccgggc gccgggagtt
      301 ctcgacgccg gatagggtgc cttttgtgag tgagatccga ggtgtaataa cggcattggc
      361 caccgctttc cggcccgacg gcgagctcga tatcgactcg acccggaagc tggccgccta
      421 tctcgtcgag aacggcagcc acggcctggt cgtggccggt tcaaccggcg agtcgtcaac
      481 cctggaagac gacgagaaga tcgatctcac cagggcggta gtggaagagg tcgggccgga
      541 agtgacggtg atcgtcggtt ccggttcgaa tgacacccgt cactcggtcg agctgaccaa
      601 gatggcggcg gaagccggcg ccgacgccgc cctgatcgtg acgccgtact acgtgcggcc
      661 caaccgggca ggaattcgcc ggcacttcga agccgtcgct gaaggcagcc ccggctttcc
      721 gatcgtcgct tacaacatcc cttcccgcac cgggatcaac atgcctcccg aattccttgc
      781 cgaactggcg cagatcgacg aagtcaaggc ggtcaaacag gccaatgacg acgatctcgg
      841 gccgatcgag gggctcgacg tgctggccgg caacgacggc acctttcttc cgacactgaa
      901 gtttggcggg gcgggcggca tcctcgttgc ctcacacctc gacaacggcc gcatgcgaac
      961 gatctgggat ctctggcagg agggccgtca cgacgaggcc gaggccctcg acaaccagct
     1021 tcagcccctt tacgaggcga tgggcgtcgt cgtcaacccg attccggtca aggccgcgct
     1081 cgccgcccgc ggcctgcttc ctccgaccat gcgcctgccg ctggtcgagc cgaccgatca
     1141 ggaacgtgac gccgtcgaag agctcctcgc cgaggccggt gtgcccgccg catgagccag
     1201 gaccggatca agctgcttcc cctcggcgga ctgggggaaa tcggcaagaa catgatggtg
     1261 atcgagtacc gcggctcgat cgtggtggtc gacaccggtc tgatgttccc gaccccggag
     1321 atgctcggca tcgacctcgt cctgccggat ttcgaatacc tgcgcgaccg ggccgatgac
     1381 atcgaggcga tcatcctcac tcacggccac gaggaccatg tcggcgcgct cccgtacgcg
     1441 ctgcgggaga tcggctttcc caaggtcatc tacggcggca tgctcacggt gggaatggtc
     1501 cgttcgaaac tggacgagca caagcttggc agcgccccgc tgaccgaact gccggccggc
     1561 cagaaagtca gcgccgggcc cttcgagatt gaactggttc acctctcgca ttcgatcccc
     1621 gacatgcgcg gggtgatcat cggcaccgat ctcggcaaga tcttcatgac cggcgactac
     1681 aagttcgacc agaccccggt cgatggccgc ccggccgacg tggcccgcct tgcggcgctt
     1741 ggagacgaag gcctgctcct gctctgcggc gattcgacca acgccgaccg gcccggcgta
     1801 tcgccttctg aatccagtgt cggcccggcc ctctatgaca ccttctcgcg ctgcaagggg
     1861 cggatcatcg tcacctcgtt cgcgtccaac atccaccgcg ttcagcaggt gattgatgcc
     1921 gccgagcggc tcgaccgcaa agtcgccctg gttggccgtt cgatgcgcaa gaacttcaac
     1981 atcgcctcca atctcggaat ggcgaacgcc cccgaggggc tgctgatcca gccgcgggag
     2041 atcgaggatt ttcccgacga gcgggttgtg gtgatctcga ccggctccca gggcgagccc
     2101 ttctcggccc tgcggcgcat ggccaacaac gaccaccggg acgtggaact ccactcgggg
     2161 gacacggtcg tcttctccgc gaccccggtg cccggcaacg aacgggccgt gaacgagacg
     2221 atcgaccgca tctacgaact cggagcgacc gtgatcacgc ccaaggacgc cccgatccac
     2281 gcttccggcc acggataccg ggaagagatc aagatgatgc tgaacctgac ccggccgaag
     2341 tacgtgatgc cggttc
//
LOCUS       DAOQMF010000266         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28716_length_2353_cov_3.196693, whole genome shotgun sequence.
ACCESSION   DAOQMF010000266 DAOQMF010000000
VERSION     DAOQMF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28716_length_2353_cov_3.196693"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1329
                     /locus_tag="PLV77_05320"
     CDS             214..1329
                     /locus_tag="PLV77_05320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC15299.1"
                     /translation="MRNGRTVKYESLRIRPFGMHPAHRGYRDLGDYRRNQVRYGIAFA
                     TITAICFAATLVSSEDVQWGLYLGGWALALACVGLAWANPALWLLAPFGALGGAILVR
                     AGTDGLDAGIGPLLLIPMLAVAVYGSRRALIAMIACVAGAVIIGQLVWDGGNSEMSPI
                     WRQDIVLAMLATALGVAMQDLVTRMRSERNLAEERGRIIEELALTDPLTGIDNRRGWE
                     RMIQRSVGQARRHGQPLCLAMLDLDHFKLYNDTFGHQAGDDFLRRVALDWEERTRIED
                     HVARYGGEEFVVTLPNTGLAGAKVIVERLRTGLPLPLEDRESTITCSAGIAEWDGSET
                     VRELVARADQALYLAKEGGRDRTVTVEASARETGFRA"
     gene            complement(1348..1785)
                     /locus_tag="PLV77_05325"
     CDS             complement(1348..1785)
                     /locus_tag="PLV77_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15300.1"
                     /translation="MTDEPLDQPPPNEPPQDEAGGPPPLTDEERAMLEAQMKEIRVED
                     LLTQTVASVLNLSARRIVKEDEVDLDQARIGIEAVRAIVALLPAEVGQAIAEPLSQVQ
                     LLYAQRSGETGEPGGEEGATGGAGPSNEPPAGHGPSKLWTPGS"
     gene            complement(1795..2052)
                     /locus_tag="PLV77_05330"
     CDS             complement(1795..2052)
                     /locus_tag="PLV77_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15301.1"
                     /translation="MNRLVPLLAVFFLSCAPLFGCTSSRTTEVSNGASANGVPQNLVL
                     RSYEVPNNGAQRMRLDLESGKPIEEIYRETVAETAHSYAGP"
     gene            complement(2108..>2353)
                     /locus_tag="PLV77_05335"
     CDS             complement(2108..>2353)
                     /locus_tag="PLV77_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15302.1"
                     /translation="VDAGEADAGGSDAGADGGASADAGTGADAGEVPDGGAAHRELTV
                     GCGCTGAPAWWLVVAALALVGRNPVRRRASVRNRRGD"
BASE COUNT          355 a          791 c          802 g          405 t
ORIGIN      
        1 ggaaaaccgg aagaagattc aggaaaacga ccggctccgg cgcgcgcctc gatggcgtcg
       61 agccgggcgg agagttcctc ccttagattc ctgatttcct tgagaatttc ttttgaacgg
      121 tctttagcca ccaggctccc gatattatcg gcacgtgcag gagctttgaa caccgaatgg
      181 taagtcctgc cgccagtgag tggcccacta taggtgagga acgggagaac cgtcaagtac
      241 gaaagcctga gaatccgtcc gttcggaatg cacccggccc atcgcggcta ccgggacctc
      301 ggggattacc gtcgcaatca ggttcgctac ggaatcgcct tcgccacgat cactgcaatc
      361 tgctttgcag cgaccctggt ttcctctgag gacgtccagt gggggctcta cctgggcggc
      421 tgggcactcg cccttgcctg cgtgggcctg gcctgggcga atccggccct ctggctgctc
      481 gcgccgttcg gggcgctcgg cggtgcgatt ctcgtgcggg cggggaccga cggtctcgac
      541 gccggcatcg ggccgctgct gctgatcccg atgctcgcgg tcgccgtgta cggatcacgg
      601 cgcgccctga tcgcgatgat cgcctgtgtg gccggcgcgg tgatcatcgg ccagctcgtc
      661 tgggacggcg gcaactccga gatgagtccg atctggcggc aggacatcgt gctggcgatg
      721 ctcgcgacgg cactcggtgt ggcaatgcag gatctggtca cccgtatgcg cagcgagcgc
      781 aacctcgccg aggagcgtgg ccggatcatc gaagagctgg cgttgaccga tcccctgacc
      841 gggatcgaca accggcgcgg ctgggagcgg atgatccagc gttcggtcgg tcaggcccgg
      901 cggcacggcc agccgctttg cctggcaatg ctggacctcg accacttcaa gctttacaac
      961 gacactttcg gccatcaggc cggggacgat ttcctcagaa gggtcgccct cgactgggaa
     1021 gagcggacca ggatcgaaga ccacgtcgcc cgatacggcg gcgaggagtt cgtggtgacc
     1081 ctgccgaaca ccgggctggc cggggccaag gtaatcgtcg agcggctccg caccggcctg
     1141 ccgctcccgc tcgaagaccg cgagagcacg atcacctgct cggccggcat cgccgagtgg
     1201 gatgggtcgg aaacggtgcg ggaactggtg gcccgggccg accaggcgct ctacctggcc
     1261 aaggaaggcg gccgggaccg gaccgtcacg gtagaagctt cggcccgcga aaccggattc
     1321 cgggcctgag ctggtccggc cggccggtca gctgccggga gtccagagct ttgagggacc
     1381 atggccggcg ggcggctcgt tggacgggcc ggcacctccg gtcgcgcctt cttctccccc
     1441 gggctctccg gtttcgccgc tccgctgcgc gtagagcagc tgaacctggc tgagcggctc
     1501 ggcgatcgcc tggccgacct cggccggaag cagggcgacg atcgcccgaa ctgcctcgat
     1561 cccgatccgc gcctggtcga ggtcgacctc gtcctccttg acgatccgcc gggctgaaag
     1621 gttgagcacg ctggccacgg tctgggtcag aaggtcctcg acccggatct ccttcatctg
     1681 ggcctcgagc attgcccgtt cctcgtcggt cagcggcggt ggcccgcccg cctcatcctg
     1741 cggaggctcg ttcggcggcg gctggtccag cggttcgtcg gtcatggctt cactctatgg
     1801 cccggcatag ctgtgagcgg tttcggccac ggtctcacgg tagatctcct cgatcggctt
     1861 tccggattcc aggtccaggc gcatgcgctg ggcgccgttg ttgggcacct cgtagctgcg
     1921 cagcacgagg ttctgcggca ctccgttggc cgacgcgccg ttcgagacct cggtggtgcg
     1981 gctgctggtg cagccgaaga gcggggcgca gctgaggaag aagacggcga ggagggggac
     2041 gaggcggttc atgttcgaag ctccagtgca gcgggttgcc ggtgttaccc gcggccctcg
     2101 accttcttca gtcgccgcgt cgatttctca ccgaggcccg tcgtctcacg gggttgcggc
     2161 ccacgagcgc gagcgcggcg accaccagcc accacgccgg cgcgccggtg cagccacagc
     2221 ccaccgtcag ctcgcgatga gccgcgccgc catcgggcac ctcgcccgcg tcagcgcccg
     2281 tgcccgcgtc ggccgacgcg ccaccgtcgg cacccgcatc gctgccaccc gcatcggcct
     2341 caccagcgtc cac
//
LOCUS       DAOQMF010000267         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28726_length_2353_cov_2.338120, whole genome shotgun sequence.
ACCESSION   DAOQMF010000267 DAOQMF010000000
VERSION     DAOQMF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28726_length_2353_cov_2.338120"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLV77_05340"
     CDS             complement(<1..452)
                     /locus_tag="PLV77_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC15303.1"
                     /translation="MDGVQKSREILTDLDPDQRAAVTARNEWPLLVAAGPGAGKTRVL
                     TRRIAWLIEATGIPAGQVMALTFTNAAAKEMGERLYDLIGAESIRCRLSTIHALAARI
                     VRAHAEKVGLTHDFTIFDQDAQLKLAATVLEDEGDLRNFNISVGVTDA"
     gene            complement(485..1153)
                     /locus_tag="PLV77_05345"
     CDS             complement(485..1153)
                     /locus_tag="PLV77_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15304.1"
                     /translation="MNQSTITVQTIGNLTIDRTDQTVHVDGKKVPTLLREFQLIDQLA
                     QCEAPATFEKLGRKAFAASELRTAGAVEAVAHSLRAKLTSYGARRGLVHINGNKISMF
                     TSPQKVRVQAFHVPSARYTAGPEDGSVIPDLSLCDLIWFGELPESDDRTVLEQTFHLL
                     NIDTRPNRLFAPALTVGDIVTLQPGGAEAVSYQVDHKGWIRLRGTVQAAEADLDLVEN
                     QLAA"
     gene            complement(1563..1844)
                     /locus_tag="PLV77_05350"
     CDS             complement(1563..1844)
                     /locus_tag="PLV77_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15305.1"
                     /translation="MGDYSETVAGKTGKCVVCGASTHDRSVTLNRSTGKVGHKTWRCG
                     NFEACRERRAAGKKRNRPKPGVKKACAECGGFLWHEDGCSRTTRRSPGQ"
     gene            complement(2125..>2353)
                     /locus_tag="PLV77_05355"
     CDS             complement(2125..>2353)
                     /locus_tag="PLV77_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15306.1"
                     /translation="YGRLLRYARIDGKDLGRQVIAAGWGAPYVFNEDSPPKNIGYYRT
                     AATNAKNAGRGVWGLCDGNFHSEEDQPWSGD"
BASE COUNT          345 a          691 c          791 g          526 t
ORIGIN      
        1 aaggcatccg tcacgccgac ggagatgttg aagttgcgca ggtcgccctc gtcctccaac
       61 actgtggccg cgagcttcag ctgcgcgtcc tggtcgaaga tcgtgaagtc atgggtcaaa
      121 ccgacctttt cggcatgtgc ccggacgatg cgggcggcga gagcgtgaat cgttgagagc
      181 cggcaccgga ttgattcggc gccgatcagg tcgtagagcc tttcccccat ttctttggcg
      241 gccgcgttcg tgaacgtgag cgccattacc tggccggcag gtatgccggt cgcttcgata
      301 agccatgcga tccgacgggt gagtacccgg gttttgccgg caccgggccc ggccgcgacc
      361 agtagcggcc attcgtttcg ggcggttacc gctgcccgct ggtcaggatc gagatcggta
      421 aggatctcgc ggctcttttg gacaccgtcc atggagcctc ctttcgttga aagggtcagg
      481 cggtctaggc ggcgagctgg ttttcgacca ggtcaaggtc ggcttccgcc gcctggacgg
      541 tgcctcgaag ccggatccag cctttgtggt caacctgata gctgaccgct tcggcaccac
      601 cgggctgcag ggtgactatg tccccgaccg tgagggcagg ggcaaagagc cggttggggc
      661 gggtgtcgat gttcagcaga tggaaggtct gctcgagcac cgtccggtca tcggattcgg
      721 gaagttcccc gaaccagatc agatcgcaaa gcgacaggtc cgggattacg ctgccgtctt
      781 ccggcccggc ggtgtatctg gccgagggaa catggaaagc ctggactctc actttctgcg
      841 gtgaggtgaa catgctgatc ttgttgccgt tgatgtgaac gagtccccgg cgggcaccgt
      901 aggaagtgag tttcgcgcgg aggctgtgcg cgaccgcttc cacggccccg gctgttcgga
      961 gttcgcttgc ggcgaatgct ttgcggccga gcttctcgaa agtggccggc gcttcgcatt
     1021 gggccagctg gtcgatcagc tggaattcgc gaagtaacgt tggcactttc tttccgtcga
     1081 cgtgaacggt ctggtcggtt cggtcgatcg tcaggtttcc gatcgtctga accgtgatgg
     1141 tggattggtt catgtttgct cctttgcccg gtttcgggca tgaaaaaggc ggcctgctgg
     1201 ccgcctttga atgtggatgg atgttgggtt ttcggggtgt ccgggtcagc cgatgattcc
     1261 tgcgaggctg agcgcgccaa gcgtcatcgc gatcgcgacc caagtgaacg ccagtttctc
     1321 tctggttgtg agatccggcg gcctgtggtg ctcatgcgtg tttccgccat ggcgccaggt
     1381 gagtcgaggg tcgaccctgt cggtacatgc cgagcagagg ttctcttcga tccaccagca
     1441 ggcctggccg ggctggttcc atgcccgctc cggtgccttg gtgacaacac aggcgtcgtg
     1501 ctcggtgcag ccgcacccac ggcacactcg ttccgtcatg gggccggcaa cagtggtagc
     1561 ggtcattgtc ctgggcttct ccttgtggtt cttgagcagc cgtcctcgtg ccagaggaac
     1621 ccgccacatt cggcgcaggc tttcttgaca ccgggtttcg gccggtttcg tttcttgccg
     1681 gcagcgcgac gctcccggca ggcttcgaag ttgccgcacc gccaggtttt atggccgacc
     1741 ttgccggttg accggttgag cgtcacggag cggtcatggg tgctggcacc gcaaaccacg
     1801 catttgccgg tcttgccggc aaccgtttcg ctgtagtcac ccatgagctg ccacctcggt
     1861 cgaagtcggc tgcccgtcgg gatcttcgac aggggcccgt tggctggtgt tgagcgggca
     1921 gggaggcaca tgctggccct ccggaacgcc atgacacccg ttggggcagt cctgcggggc
     1981 gccgtcgtac cagcggccgt agacgagccc gttgtcgagt acttgctcgc ccgggcccgg
     2041 gggccggtcc ttgatcgaac cggaccggtc aggttttgat ttcttgtttt tctttcccat
     2101 gtgtttcacc ttgctttcga tgggtcagtc gccggaccag ggctggtctt cttcggaatg
     2161 gaagttgccg tcgcaaaggc cccagacgcc gcggccggcg ttcttggcgt tggtggctgc
     2221 ggtccggtag tagccgatgt ttttcggggg gctgtcctcg ttgaacacgt aaggggcgcc
     2281 ccatccggcg gcgatcacct ggcggcccag gtccttgccg tcgatccggg cgtagcggag
     2341 cagccggccg tac
//
LOCUS       DAOQMF010000268         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28787_length_2350_cov_2.856209, whole genome shotgun sequence.
ACCESSION   DAOQMF010000268 DAOQMF010000000
VERSION     DAOQMF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28787_length_2350_cov_2.856209"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1243)
                     /locus_tag="PLV77_05360"
     CDS             complement(35..1243)
                     /locus_tag="PLV77_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNC15307.1"
                     /translation="MKVGSGNGVKGSVSGGLPLSPDDVARALRPLEHASMLPPAAFVD
                     QAVFEWEQKHLFHDGWICLGHVSQVSEQGKFIRREVGPDSVVVIGGEGGRPHAFLNVC
                     RHRGARVIEEADGQVKRRLQCPYHAWSYALDGTLVAAPHMDGVEDFDKSCYGLIPVRM
                     ATIGGLVLVDLGGEAPEPDAHVGELIEHLDRYRTAELVSGGRIDYEVAANWKGIAENY
                     NECLHCPGVHPELNALSNYMSGEEMQGSGSWCGGSMFITAEDAATMGKDGGHAGNRAR
                     IEGLTEEDDKTILYFALFPNALVSMHPDYVMLHTLYPRAPGRTEVICEWFFERRAVEA
                     GDFDPSDAIEFWDQTNRQDWYVCELTQKGVGTTGYTAGRYSAEEGTVHEFDNLVAGKY
                     MEALNGKVKA"
     gene            1378..>2350
                     /locus_tag="PLV77_05365"
     CDS             1378..>2350
                     /locus_tag="PLV77_05365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC15308.1"
                     /translation="MNETVTSPDLEGLDEAAVDLRQRARKFVDEVLIPLEQEAELLHG
                     RLPDEAVRMVREKALAAGLTGGLHKPEHGGQGWSKVEWFLVEEQLGRSTNAISWHIPN
                     GYNFWDLASGKHVEKWLKPLLRGETRDAYAVTERNAGSDPSGIETRAVRTDGGFRING
                     EKWFVTSGDVADVLVVMANLEEDGELLPTLFVVEKSLGGVETIEDPGFTHSYPDGHPT
                     IRFVDVEVGEDDVIGGIGGGDEVQKAWFAEERLGIAARGIGSMWRLLEETVDWAANRE
                     QGGSRILDYQGVSFPLADSAADAAAGRLLTFELARMIDAGADPKICHN"
BASE COUNT          391 a          788 c          771 g          400 t
ORIGIN      
        1 acctttccgt tcgcagggct cgccccgttc ccgctcatgc cttcaccttt ccgttcagcg
       61 cttccatgta cttgccggca accaggttgt cgaactcatg gaccgtccct tcctcggccg
      121 agtagcggcc ggccgtgtaa ccggtggtgc ccaccccctt ctgggtcagc tcgcagacgt
      181 accagtcctg ccggttggtc tggtcccaga actcgatcgc atccgagggg tcgaagtcac
      241 cggcctcgac cgcgcgccgc tcgaagaacc attcgcagat cacctcggtc cggccgggcg
      301 cgcgcgggta aagggtgtgc agcatcacgt aatcgggatg catcgagacc agcgcgttgg
      361 gaaagagcgc gaagtagagg atggtcttgt cgtcttcttc ggtcagtccc tcgattcgcg
      421 cccggttgcc ggcgtggcca ccgtccttgc ccatcgtcgc ggcgtcctcg gcggtgatga
      481 acatcgagcc cccgcaccag gaaccggaac cctgcatctc ttccccgctc atgtaattcg
      541 aaagcgcgtt cagctccggg tggactccgg ggcagtggag gcattcgttg tagttctcgg
      601 cgatgccctt ccagttcgcg gcgacctcgt aatcgatcct ccccccgctg accagttcgg
      661 cggtgcggta gcggtcgagg tgttcgatca gctcgccgac gtgggcgtcc ggctccggtg
      721 cctcgccgcc caggtcgacc aggaccaggc cgccgatcgt cgccatccgg accgggatca
      781 ggccgtaaca ggacttgtcg aaatcctcga ccccgtccat gtgcggggcc gcgaccaggg
      841 tcccgtccag cgcgtaggac caggcgtggt acgggcactg gagccggcgc ttgacctgac
      901 cgtctgcttc ctcgatcacc cgggcgccgc gatggcggca gacgttgagg aacgcatggg
      961 gccggccgcc ctcgccgccg atcacgacca ccgagtcggg cccgacctcg cggcggatga
     1021 actttccctg ctcgcttacc tgagagacat ggccgaggca gatccagccg tcatggaaga
     1081 gatgcttttg ctcccactcg aagacggcct gatcgacgaa ggcggccggc ggcagcatcg
     1141 aggcatgttc caggggccgt agcgcccggg caacgtcatc cggactcagg gggagcccgc
     1201 cggagacgct gcccttgacg ccgttgccgc tgcccacctt catctcgatc ctccggttgt
     1261 gccgacgggc tcgggatggg cccgcctgca gcgatactag cgccggttga ccttgtagtc
     1321 aatcgcatgt actctggaga cattccgctt gaccaaacga gatcggcgag ggagtacatg
     1381 aacgaaaccg tcacaagccc cgatctggag ggcctcgacg aagcggcggt cgacctccgg
     1441 caaagagccc ggaaattcgt cgacgaggtc ctgatcccgc tcgagcagga agcggagctt
     1501 cttcacggcc ggctgcccga cgaggcggtt cggatggtca gggaaaaagc cctcgcggcc
     1561 ggtctcaccg gcgggctcca caagcccgaa cacggcggcc agggctggag caaggtcgag
     1621 tggttcctgg tcgaggagca gcttggccgt tcgaccaacg cgatctcctg gcacatcccc
     1681 aacggttaca acttctggga tctcgcttcc ggcaagcacg tcgagaagtg gctgaagccg
     1741 ctcctgcgcg gcgagacgcg cgacgcctac gcggtgaccg aacggaacgc cggctcggac
     1801 ccctcgggga tcgagacccg ggcggtccgg accgacggcg gcttccggat caacggagag
     1861 aagtggttcg tgacttcggg cgacgtggcc gacgtgctgg tcgtcatggc caacctcgaa
     1921 gaggacggag agctcctccc cactctcttc gtggtcgaga aatcgctggg cggagtcgag
     1981 acgatcgagg accccggctt cacccactcc tatccggacg gccacccgac catccgcttc
     2041 gttgacgtcg aggtcggcga ggacgatgtg atcggcggga tcggcggcgg cgacgaggtc
     2101 cagaaagcct ggttcgccga ggagaggctc ggcatcgcag ctcgcgggat cggttcgatg
     2161 tggcggctgc tcgaggagac cgtcgactgg gccgcgaacc gcgagcaggg cggctcccgg
     2221 atcctcgact accagggggt ttcgtttccc ctggcggact cggctgccga cgccgccgcg
     2281 ggccgcctgc tcaccttcga gctggcgcgg atgatcgacg ccggcgccga cccgaagatc
     2341 tgtcacaaca
//
LOCUS       DAOQMF010000269         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_28972_length_2343_cov_2.950612, whole genome shotgun sequence.
ACCESSION   DAOQMF010000269 DAOQMF010000000
VERSION     DAOQMF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28972_length_2343_cov_2.950612"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PLV77_05370"
     CDS             <1..1473
                     /locus_tag="PLV77_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC15309.1"
                     /translation="FGGPTPLAARGYEVKLPLTQIGQLAEQSEVDISGVEIGRVKSID
                     LGEGGEEGSAIVTLNLEPEYAPIPADTRAILRAKSLLGEAYIELTPGDKRNGMLDDGG
                     ELPPAQVAKSVQLDEIFRTFDEKTRQAFMQGAIDNSIAISGRGATLNQTLGILPGTVT
                     SLTDVLQVLNEQDQDVSKLIKNTGVVFDALSKRQGELSGMIRNTDTVFKTLADRDQEL
                     KDFFRVFPTFLRESRATQERLGDFSDFATPIVRKLVPVAKQLSPTFKASANLAPVSER
                     LYTNLKPVIRKAPRAFPSLRAFLDEDAPTLLGRLPDYLAEFNPFLETGSLYRKEIAGL
                     IGNAASATQPSISGGAHYVRAGVRLGPETYTSYPGTGPSNGRVNPYTAPGGNLDFSSS
                     GGMKVFSSANCTGSVNATIVPWSGLTPVQQAQFGANLRYGFSAAQQEDWYTRAITYLM
                     AGQNQTNNVPASACNQQAPFTPIGDESQPPTQYLHVFREP"
     gene            1716..>2343
                     /locus_tag="PLV77_05375"
     CDS             1716..>2343
                     /locus_tag="PLV77_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15310.1"
                     /translation="MKKLVAIVVALIASLSMAASAQAANETVEGTTNISAINGPLYQT
                     KAVPVNLMIRAEVTTPAESPKVNPLKNSKITFPAGVSFNPNNSKTPACTDNMLSVTSN
                     LADPSGVVASCEDSVVGTGTSAIVLAKVNLPETLIEDPILVLFNAGTDSKGQAKVKIY
                     GYSKTTNVGILMHGTLVKGVLDVAVPVLSDDSAVKYYQFDLPGPGLDRD"
BASE COUNT          473 a          787 c          689 g          394 t
ORIGIN      
        1 ttcggcggcc cgaccccgct ggcagcccgc ggttatgaag tcaagctgcc gctgacccag
       61 atcggccagc tggccgaaca gtccgaggtt gacatctccg gcgtcgagat cggccgggtc
      121 aagtcgatcg acctcggaga agggggcgaa gaaggctcgg ccatcgtgac cctgaacctg
      181 gaaccggagt acgcgccgat cccggcggac acgagggcaa tcctgagggc gaagagcctg
      241 ctcggcgagg cctacatcga actcaccccg ggtgacaagc ggaatggcat gctggacgac
      301 ggcggtgagt tgccgcccgc ccaggttgcg aagtcggtcc agctcgacga gattttccgc
      361 acatttgacg agaagaccag gcaggcattc atgcagggag cgatcgacaa ctcgatcgcg
      421 atcagcgggc gcggcgcgac cctgaaccag acgctcggca tcctgccggg cacggtgacc
      481 tcgctgaccg acgtgctcca ggtcctgaac gaacaggacc aggatgtgtc gaagctgatc
      541 aagaacaccg gcgtggtctt cgatgcgctg agcaaacggc aaggcgagct cagcggcatg
      601 attcgcaaca ccgacaccgt cttcaagacc ctggccgacc gggaccagga attgaaggac
      661 ttcttccggg tcttcccgac cttcctgcgc gagtcccggg ccacccagga gcggctcggc
      721 gacttctcag attttgccac cccgatcgtc cgcaaactgg tacctgtcgc caagcagctt
      781 tcgcctacct tcaaggcctc ggccaacctg gctccggtca gtgaacggct ctacacgaac
      841 ctgaagccgg tgatccgcaa ggcgccgcgg gcgttcccgt cgctaagggc cttcctcgac
      901 gaggatgccc cgacgctgct cggcaggctg cccgactacc tggccgagtt caatccgttc
      961 cttgaaaccg gcagtctcta ccgcaaggaa atcgcgggac tgatcggcaa cgcagcatcg
     1021 gcaacccagc cctccatttc cggcggtgcg cactacgtcc gggccggcgt ccgtcttggg
     1081 ccggagacct acacctctta cccggggacc ggcccatcga acggccgggt caacccgtac
     1141 acggccccgg gcgggaacct tgacttttcc tccagcggtg gcatgaaggt cttctcttcc
     1201 gccaactgca ccggctcggt caacgcgacc atcgtgccct ggagcggcct taccccggtc
     1261 cagcaggccc agttcggggc caacctgagg tacggcttca gtgccgccca gcaagaggac
     1321 tggtacacgc gggccatcac ctatctgatg gccggccaga accagaccaa caacgtgccg
     1381 gcatccgcct gcaaccagca ggctccgttc acgccgatcg gcgacgagtc ccagccgccg
     1441 acccagtacc tgcacgtttt ccgcgagccg taaatccggg cagggttttt acttcaggcc
     1501 aggcaattgg ccggattccg ctcttggtcc ggatttggct ccctaagcga caaattggcg
     1561 atccgttgca tcatcattgc gaccgatctc caaaagcacc gcaactcaga cgccatattc
     1621 gggttcgtgt tcatgtaagc tgcgaccggt tagggagtgg gagcggctga caagtcgcaa
     1681 ccacgtgtaa tccacaggag agaagagaga cccagatgaa gaagcttgta gccatagtcg
     1741 tggcgcttat tgcgtcactt tcgatggcgg cctccgccca ggcggcgaac gagaccgtcg
     1801 aaggcaccac caacatcagc gcgatcaatg gtccgctgta ccagaccaag gcagtgccgg
     1861 tgaacctgat gatccgtgcc gaagtcacca ctccggccga atcacccaag gtgaacccgc
     1921 tgaagaactc gaagatcacc ttccccgcgg gcgtgagctt caacccgaac aacagcaaga
     1981 ccccggcctg caccgacaac atgctcagcg tcacgtcgaa cctggccgat ccgtcgggtg
     2041 tcgtcgcgtc ctgcgaggac tccgttgtcg gcaccggtac ctcggcgatt gtccttgcca
     2101 aggtcaacct gccggagacc ctgatcgagg acccgatcct ggtcctcttc aacgccggca
     2161 ctgacagcaa gggccaggcc aaggtcaaga tctacggtta ctcgaagacc accaacgtcg
     2221 gcatcctgat gcacggcacg ctggtcaagg gtgttctcga cgttgccgtc cccgttctct
     2281 cggacgactc ggccgtcaag tactaccagt tcgacctccc gggcccgggc ctggaccgcg
     2341 atg
//
LOCUS       DAOQMF010000270         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29015_length_2341_cov_3.423447, whole genome shotgun sequence.
ACCESSION   DAOQMF010000270 DAOQMF010000000
VERSION     DAOQMF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29015_length_2341_cov_3.423447"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLV77_05380"
     CDS             <1..291
                     /locus_tag="PLV77_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15311.1"
                     /translation="EGSRPSFPPSRRDAIILGGTIFDLLEDYRGGGACELLIGRLRRD
                     GPVANLEVAFDLRIREVEEMWREYLRDLARPQPDSTSLPSPEELLGPIQPEP"
     gene            complement(304..1452)
                     /locus_tag="PLV77_05385"
     CDS             complement(304..1452)
                     /locus_tag="PLV77_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15312.1"
                     /translation="MRRTVGVLAAFGLAVMMLFGAQSASAAGKTVTISGKAFIFNYMD
                     TPIPGATIKVREFPKLSAVTNDQGDYELTVPDDANVTPYIEPGGPVNLTRYNKDGSLK
                     GILDNTHWNTIDLQTFHTRGQDLENANFQTPRDAEYNGLKALLAIPSSPDGRPAQCAI
                     VTTSSARNVRGVDYLTYWNNTPHGVAGATSIEYPALDGPVYFNDSVIPDATKTESSGD
                     GGIIWPIVPTGTYRIVTTSPDTQFASFLATCKPGRVINANPPWGAYELSPGEKPLGAS
                     NVAAKLVSAKAVRKSKTRRQLSLVLNSGENIDATVTLAAGGMTALRTLPLKPGNKTLT
                     FNLGAKVKAKQAKVTVKLTDASGVSFTTVKKVNVPKTIKKKNGKGKRK"
     gene            complement(1536..>1843)
                     /locus_tag="PLV77_05390"
     CDS             complement(1536..>1843)
                     /locus_tag="PLV77_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15313.1"
                     /translation="AEPALPDPVYFNEQVIPDSSLTETSIDGGIIWPVVETGTYTITT
                     TSPTAGFASFTATCAPGRVVNANPPWGAFELVGAGEEPDGGPEFPAGCRVGAVPSAT"
     assembly_gap    1844..1853
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1854..>2341)
                     /locus_tag="PLV77_05395"
     CDS             complement(<1854..>2341)
                     /locus_tag="PLV77_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15314.1"
                     /translation="GIETPGEKTVVLSGRAYEFGHMDTMISGASIRVREFPELAAVTD
                     ESGDFRLEVPDQALVTPYIESGSGTNIRRPREGEPFEVETHWNEIDLQTFQTDGRDLF
                     NVNFQTPPDFEFEALKALLQVPSGEDGRPLKSVIVTTACARNVRGVDYDTFWLRTPHG
                     VA"
BASE COUNT          358 a          720 c          805 g          448 t
ORIGIN      
        1 gagggctccc gtcccagttt cccgccctcc cgccgtgacg cgatcatcct cgggggcacg
       61 atcttcgacc tgctcgagga ctaccgcggc ggcggcgcct gtgagcttct gatcggccgc
      121 ctgcgccgcg acggcccggt cgccaacctc gaggttgcct tcgacctccg gatcagggag
      181 gtcgaggaga tgtggcgcga atacctcagg gacctggcca ggccccagcc ggattcgacc
      241 tcgctgccct caccggagga gctgctcggt ccgatccagc cggagccctg accggggcga
      301 gcgctacttg cgcttgccct tcccgttctt cttcttgatg gtcttcggaa cgttcacctt
      361 tttgacggtg gtgaacgaga cgccggaagc gtcggtcagc ttgacggtga ccttcgcctg
      421 cttcgccttg acctttgcgc cgaggttgaa ggtcagcgtc ttgttgcccg gcttcagggg
      481 cagcgtccgc agtgcggtca tgccgccggc cgcaagcgtc acggtggcgt cgatgttctc
      541 accggaattc aagaccaggg agagctgacg cctggtcttg ctcttgcgga cagccttggc
      601 cgaaacgagc ttggcggcca cgttgctggc gccgagcggc ttctcgccgg ggctgagctc
      661 gtaggcgccc cacggcgggt tggcgttgat cacgcggccc ggcttgcagg tcgccaggaa
      721 gctggcgaac tgggtgtcgg gcgaggtggt gacgatccgg taggtgccgg tcgggacaat
      781 cggccagatg atgccgccgt ctccggaaga ctcggtcttg gtcgcgtcgg ggatgaccga
      841 gtcgttgaag tagacagggc cgtcgagggc cgggtactcg atcgaggtgg caccggccac
      901 tccatgcgga gtgttgttcc agtaggtcag gtagtcgacg ccccgcacgt ttcgggcaga
      961 cgaggtggtg acgatcgcgc actgggccgg gcggccatca ggggaagaag gaatcgcaag
     1021 cagcgccttg aggccgttgt attcggcgtc gcgaggcgtc tggaagttcg cgttctcgag
     1081 atcctggccg cgggtgtgga aggtctggag gtcaatcgtg ttccagtggg tgttgtcgag
     1141 gatgcccttg agcgagccgt ccttgttgta gcgggtcaga ttcaccgggc cgccgggctc
     1201 gatgtacgga gtgacgttgg cgtcgtcggg aaccgtcagt tcgtagtcac cctgatcgtt
     1261 ggtgaccgca gaaagcttgg gaaactcgcg gaccttgatc gtggcccccg ggatcggcgt
     1321 gtccatgtag ttgaaaataa aagccttgcc gctgatcgtc acggtcttgc ccgccgccga
     1381 cgccgactgg gcgccaaaca gcatcatcac ggccagcccg aaagcggcca gcacgccaac
     1441 agttctcctc aacatcaatc tcctgctttc tcctgcccgg gtcgtcccgg acggtaatga
     1501 gtgtggttcc ggaatctaca acaggttgga ccacatcaag ttgcggacgg gaccgctccc
     1561 acccggcacc cagccgggaa ttcaggcccg ccgtccggtt cctcaccggc cccaaccagc
     1621 tcgaaggcac cccagggcgg gttcgcgttg acgacccggc cgggggcgca ggttgccgtg
     1681 aagctggcga agccggcggt cggggaggtg gtggtgatcg tgtaggtgcc ggtttcgacc
     1741 accggccaga tgattccgcc gtcgattgag gtctcggtga ggcttgaatc ggggatcacc
     1801 tgttcgttga aatagaccgg atccggtagg gccggctcgg cggnnnnnnn nnnccgccac
     1861 accgtggggc gttctcagcc agaaggtgtc gtaatcgacg ccgcgcacgt tgcgggcgca
     1921 ggcggtggtc acgatcaccg acttgagcgg ccggccgtcc tccccggagg gaacctgaag
     1981 caatgccttc agcgcctcga actcgaagtc gggtggggtc tggaagttca cgttgaacag
     2041 gtcccggccg tcggtctgga aggtctgcag gtcgatctcg ttccagtggg tctcgacttc
     2101 gaagggctcg ccctcccggg ggcgccggat gttggtgccg ctgccggatt cgatgtatgg
     2161 ggtcaccagg gcctggtccg gcacctcgag ccggaagtcg ccggactcgt cggtcacggc
     2221 ggccagttcc gggaactcgc ggacccggat gctcgccccc gagatcatcg tgtccatgtg
     2281 accgaactcg taggcccggc cgctcaggac gaccgtcttt tccccgggcg tttcgattcc
     2341 g
//
LOCUS       DAOQMF010000271         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29033_length_2340_cov_4.143982, whole genome shotgun sequence.
ACCESSION   DAOQMF010000271 DAOQMF010000000
VERSION     DAOQMF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29033_length_2340_cov_4.143982"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2340
                     /locus_tag="PLV77_05400"
     CDS             <1..>2340
                     /locus_tag="PLV77_05400"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HNC15315.1"
                     /translation="IAGSGPGGKVTKADVLAAGDGSGAVANREEKALRGPAGMLAKAM
                     DESRSIPTATSFRTVAVDTLDAKRKGLNGALAERGMKVSFTHLVAWAIVRAARKWPVM
                     ARHFEEKDGKLFSVSDGQVNLGIAVDVEKKDGSRSLMVPAIKGADQLDFPAFHAYYED
                     LITKTRENRLTPDDFTGTNITLTNPGGIGTVASVPRLMSGQGTIVAAGSIAYPPEWSH
                     ADQEKIEQLGVSKVMTLTSTYDHRIIQGAESGNFLKTLDGLLAGGDEFYELVADDLGV
                     DRTVLTAAQAKAKQAPPLSAAAAPAASAIAEAPSHELLQAVQAATSLLKAYRTHGHLA
                     AHLDPLGAKPKGDPALDPETVHLTPELMAQVPASILRIGVPGETLLEALPRMKAAYTG
                     SIGYQFEHISSHSQRVWLREMVETGAHRKALEQDEQKRLLGRLIDVFEFERFLEKAYL
                     GQKMFSIEGLDSVVTMLDELTTLAARGGAKEVVLGMAHRGRLSVLAHTVRRPAEAIFA
                     EFEGGKRIEDVKAVAAIPHGGTGDVKYHYGHEGVAEHHDGSKIKVHLYPNPSHLEFVN
                     PVITGATRYSQDVIDGSELTHDPASAVPVVLHGDAAFPGQGVVAETFNLQSLEGYSVG
                     GTIHIIENNQIGFTTDPNEGRSTPYAADMAKGFNVPIIHVNADDVEACSQAMRLAMAY
                     RERWNRDIVIDVIGYRRFGHNETDEPAYTQPLMAAEIKKHKPVSEIYTEQLIEQKVVT
                     ADEVGAAAEKRRASLKAALDELRKKMDTGVYEDPTVTQV"
BASE COUNT          463 a          829 c          714 g          334 t
ORIGIN      
        1 agatcgcggg cagcggcccc ggtggcaagg tgaccaaggc cgatgtgctc gcggcaggtg
       61 atggtagcgg cgccgtcgcc aaccgcgaag agaaggcact tcgcggcccg gccgggatgc
      121 tggccaaggc gatggacgag agccgctcga tccctaccgc gacctcgttc aggaccgtcg
      181 cagtcgacac cctggacgcc aagcggaagg ggctcaacgg tgccctcgcc gaacgcggca
      241 tgaaggtttc cttcacccac ctggtcgcct gggccatcgt ccgggcagcc cggaagtggc
      301 cggtcatggc ccgtcacttc gaggagaagg acggcaagct cttctcggtc tccgacggcc
      361 aggtcaacct cggcatcgcg gtcgacgtcg agaagaagga tggctcccgc agcctgatgg
      421 ttccggcgat caagggtgcc gaccagctcg acttcccggc cttccacgcc tactacgaag
      481 acctgatcac caagacccgt gagaaccggc tgacccccga cgacttcacc ggcaccaaca
      541 tcaccctgac caacccgggc gggatcggca ccgtcgcctc ggtgccgcgg ctgatgagcg
      601 gccagggaac gatcgtcgcc gccggctcga tcgcctaccc gccggagtgg tcacatgccg
      661 atcaggagaa gatcgagcaa ctcggcgtct cgaaggtgat gaccctgacc tcgacttacg
      721 accaccggat aatccagggg gccgagtccg gcaacttcct caagaccctc gacggcctgc
      781 tggccggcgg tgacgagttc tacgaactgg ttgctgacga cctgggagtc gatcgcaccg
      841 tacttaccgc cgcccaggcg aaggcgaagc aggcacctcc gctctcggcc gctgccgcgc
      901 cggccgccag cgcaattgcc gaagcgccca gccacgaact gctccaggcg gttcaggcag
      961 cgacctcgct gctcaaggcc taccgcaccc acggtcacct tgccgcccac ctcgacccgc
     1021 tcggggccaa acccaagggc gatcccgcgc tggacccgga gaccgtgcac ctcacccccg
     1081 agctgatggc ccaggtcccg gcatcgatcc ttcgcatcgg cgtgcccggc gaaaccctgc
     1141 tcgaagcgct gccgcggatg aaggccgcct acacgggctc gatcggctac cagttcgagc
     1201 acatctcttc ccacagccag cgggtctggc tgcgcgagat ggtcgaaacc ggagcccacc
     1261 gcaaggcgct cgaacaggat gagcagaagc gcctgctcgg ccgcctgatc gacgtcttcg
     1321 aattcgagcg tttcctcgag aaggcctacc tcggccagaa gatgttctcg atcgagggcc
     1381 tcgactcggt cgtcaccatg ctcgacgaac tgaccaccct cgccgcccgc ggcggagcca
     1441 aggaagtcgt gctcggcatg gcccaccggg gccggctctc ggtgctcgcc cataccgtgc
     1501 gcagaccggc cgaagcgatc ttcgccgagt tcgaaggcgg caagcggatc gaggacgtga
     1561 aggccgtggc cgcgatccct cacggcggca ccggtgacgt caagtaccac tacggccacg
     1621 aaggcgtggc cgagcatcac gacggcagca agatcaaggt tcacctctac ccgaatccga
     1681 gccacctcga gttcgtcaac ccggtcatca ccggcgcgac ccgttactcg caggacgtga
     1741 tcgacggcag tgaactcacc catgacccgg ccagtgcggt tccggtcgta ctccacggcg
     1801 atgcggcctt ccccggtcag ggtgtggtcg ccgagacctt caacctccag tcgctggagg
     1861 gctactcggt cggcggcacc atccacatca tcgagaacaa ccagatcggc ttcaccaccg
     1921 accccaacga aggccgctcc accccgtatg cggctgacat ggcaaagggc ttcaacgtgc
     1981 cgatcatcca cgtcaacgcc gatgacgtcg aagcctgctc gcaggcgatg cggctggcga
     2041 tggcctaccg cgaacgctgg aaccgcgaca tcgtgatcga cgtgatcgga taccgccgct
     2101 tcggccacaa cgaaaccgac gagccggcct acacccagcc gctgatggcc gcggaaatca
     2161 agaagcacaa gccggtttcc gagatctata ccgaacaact gatcgaacag aaagtggtca
     2221 ccgccgacga agtcggcgcc gccgcggaga aacgccgcgc ctcgctaaaa gcggctctcg
     2281 acgagctgcg caagaagatg gacaccgggg tctacgagga tccgaccgtc acccaggtcg
//
LOCUS       DAOQMF010000272         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29299_length_2329_cov_3.334213, whole genome shotgun sequence.
ACCESSION   DAOQMF010000272 DAOQMF010000000
VERSION     DAOQMF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29299_length_2329_cov_3.334213"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLV77_05405"
     CDS             complement(<1..362)
                     /locus_tag="PLV77_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15316.1"
                     /translation="MTFAAVMLLLLGVLNVIYGIAALDKANVLVNDTRLILSDLNTMG
                     WVTIALGVIQLIGGFSLLGGNAFGRIIGVIGGTLGAIGALLSVGGAYPWWSLAIFALC
                     VWVTWGIIVFGEDENAGY"
     gene            628..>2329
                     /locus_tag="PLV77_05410"
     CDS             628..>2329
                     /locus_tag="PLV77_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15317.1"
                     /translation="MLEAPAGYSKTTTLRLWDEADKRPFAWVRCETRYNDPALLANVI
                     VSSLGETSIDPGLHELLRSPVPDLNLLLERVASAIRGLDPFVLVIDDAHLLESDDAWQ
                     VLYSVISALPEGSQVAVATRVRPGLPVGRLRARGELFEFGMNDLALTRGESRELLAGL
                     DLDLGERADEIHEKSEGWPAALYLAGLAIRQGSEARPETVEFDGGDRVVVEYFRDEFI
                     KDMPQEKARFLMETAILEELTGPLCDAVTGRRDSAEMLNELAGENTLVVPLDRNNSRF
                     RYHHLFRDMLRSEMNLRDPGAKPGLHRRAAVWLAANGDIPRATDHAIAAGDFELAGAC
                     IWLDLPDLLSRGRVATIDRWFETIGDDRIAMIPGLLLARAHREIAMGRGDETFYWLSV
                     AEREIAEDSPLFGDVLTLRATTGINGARGMIADGERAAGLMSPTSPFQVAAKLYVGIG
                     LGLTDQENASAVLRESAREAAMISGIIQAIALTELALLELDSGRVDAAHDYASRARDV
                     VERSGLRILGVMSLVYVVLALTKAERGRVEEAIVDLETSSRIGEGILDYIVWHEAQLV
                     L"
BASE COUNT          411 a          747 c          788 g          383 t
ORIGIN      
        1 cagtaaccgg cgttctcatc ctcgccgaac acgatgatcc cccaggtgac ccagacacag
       61 agggcgaaga tcgccagtga ccaccagggg taggcgccac cgaccgagag cagtgcgccg
      121 atcgccccca gagtccctcc gatgacgccg atgatccggc cgaaagcgtt gccgccgagc
      181 agcgagaacc cgccgatcag ctggatcacg ccgagcgcga tcgtcaccca gcccatcgtg
      241 ttgaggtcgc tcaggatcaa gcgggtgtcg ttgaccagga cattcgcctt gtccagcgcg
      301 gcgatcccgt agatcacgtt cagtacgccg agtaagagca gcatcactgc cgcaaacgtc
      361 acccttccgg ctcctcgcat atgtgcagct cccttcgcgg ccgaatcggc ccttggatgg
      421 atttcctgcc cggacgctaa gccggtcgaa gatcgaaggc atcacctgaa ccgggtgaat
      481 cgcgggcgcg tattcttacc ctgatggccc ctacgacagg cacggcgggg aagggctcca
      541 gggctgagga tcgggtcggc gggaggctgc tgcccgcgaa ccaggctgat cgaagccctg
      601 aacggggcgc ggtcggttcg cgtggtgttg cttgaggcgc cggccggata ctcgaagacg
      661 accacgctga ggctctggga cgaggcggac aagcgaccct tcgcctgggt ccgctgcgag
      721 acccgttaca acgaccccgc cctgctcgcc aacgtcatcg tgtcgtcgct gggggagacc
      781 agcatcgacc ccggactcca tgagttgctg cgctcgccgg tcccggacct gaatctcctg
      841 ctcgagcgag tcgccagcgc gatccgcggg ctcgatccct tcgtgctggt aatcgacgat
      901 gcccatctac tcgaatccga cgacgcctgg caggtgctct attcggtcat ttcggccctg
      961 cccgagggtt cccaggtggc ggtggcgacg agggtccgcc cgggactgcc ggtcggccgg
     1021 ttgagggcga ggggcgagct cttcgaattc gggatgaacg accttgccct gacccgggga
     1081 gaaagccgtg agctgctggc cggactcgat ctggatctcg gcgagcgggc ggacgagatc
     1141 cacgagaaat ccgagggctg gccggcggcg ctctatctcg ccggtctggc gatccggcag
     1201 ggcagcgagg cccgtccgga gacggtcgag ttcgacggcg gggaccgggt cgtggtcgag
     1261 tacttccgcg acgagttcat caaggacatg ccacaggaaa aggcgaggtt cctgatggag
     1321 acggcaatcc tcgaggagct gaccggaccg ctttgcgacg cggttaccgg ccgcagggat
     1381 tcggcggaga tgctgaatga gctggccggc gagaacacgc tcgtggtgcc gctggacagg
     1441 aacaattcgc gcttccgtta ccaccacctc ttccgcgaca tgctccggtc ggagatgaac
     1501 ctgcgggacc ccggcgcgaa acccgggctg caccgccggg ccgccgtctg gctggccgcg
     1561 aacggtgaca ttccgagggc gaccgaccac gcgatcgccg ccggggactt cgaactcgcc
     1621 ggggcctgca tctggcttga cctgccggac ctgctcagtc gtgggcgggt cgctaccatc
     1681 gatcgctggt tcgaaacgat cggggacgac cggatcgcca tgatcccggg cctcttgctg
     1741 gcccgtgccc accgcgaaat cgcgatgggc cgcggtgacg agaccttcta ctggctgtca
     1801 gtcgcggaaa gggagatcgc tgaagactca cctctctttg gtgacgtgct cacgctccgg
     1861 gcaaccacgg ggatcaacgg tgcccgcgga atgattgccg acggggaacg ggcggccggg
     1921 ctgatgagtc cgaccagtcc cttccaggtc gcggccaagc tctatgtggg tatcgggctc
     1981 ggcctgaccg accaggagaa cgccagcgcg gtgctgaggg agagcgccag ggaggccgcg
     2041 atgatctcgg gaatcatcca ggccatcgcc ctgaccgagc tggccctgct ggagctcgac
     2101 agcggccgcg tcgatgccgc ccacgactac gcttcccggg cccgggacgt ggtcgaacga
     2161 agcggattgc ggatcctcgg ggtgatgtca ctggtctatg tcgtgctggc cctgaccaag
     2221 gcagaacgcg gccgggtcga agaggccatc gtggatctcg agaccagttc taggatcggc
     2281 gaagggatac tcgattacat cgtctggcat gaggcccaac tggtcctct
//
LOCUS       DAOQMF010000273         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29375_length_2326_cov_3.051079, whole genome shotgun sequence.
ACCESSION   DAOQMF010000273 DAOQMF010000000
VERSION     DAOQMF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29375_length_2326_cov_3.051079"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLV77_05415"
     CDS             complement(<1..850)
                     /locus_tag="PLV77_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HNC15318.1"
                     /translation="MATADTETIGYTGGPQAWTVPLGVHSVEFDLYEAAGSNNGNSGF
                     VGGKGGRAKATFDVSPGQLMRLYIGGTGGFLAGGWNGGGHGFAGGGGGTDLRIGGTGL
                     GNRVLVAGGGGGVGNYGGASRGGDGGGLVGTSGTSPTAGAPGTQTEGGAGTWPGSLGQ
                     GGGGGGFGSGATRGGGGGGYYGGSTNDQEGGGGSGYGPSGTFFETGVRDGNGQAILTY
                     TTVRYALGVDILGAGQGHVSSSPAVIDCGYSGRIDCGENFAEATQVTLTATAEPGSIF
                     TGWSGAC"
     gene            1011..1259
                     /locus_tag="PLV77_05420"
     CDS             1011..1259
                     /locus_tag="PLV77_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNC15319.1"
                     /translation="MATFSISKARESLSDAVERSRTEAVFLERHGKKAAVMVSPERYE
                     EMLEAFEEIEDVAAFDQAMAEESPNIPWDEAKADLGWD"
     gene            1767..>2326
                     /locus_tag="PLV77_05425"
     CDS             1767..>2326
                     /locus_tag="PLV77_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15320.1"
                     /translation="MSVKFRGFLGLLLAAVVLSTGHADAAKNGPILFSDYTSIWKIKA
                     DGSGLKRVAKKKAWSLHASPNGKQLLYTHDGLFRMPINGGRSVNLLKRYPIVDRFAGV
                     RWASWSPNGKQIAFEGGNDGRIYLIKSNGRGLRYLFRKYRTGLLHPVWSPNGKEIAYI
                     DVHNNSSLMAVNLKSGKKRTIYPGTG"
BASE COUNT          429 a          780 c          729 g          388 t
ORIGIN      
        1 aacaggcgcc gctccagccg gtgaagatcg agccgggctc ggccgtcgcc gtcagcgtca
       61 cctgcgtcgc ctccgcgaag ttttcgccgc agtcgatgcg accgctgtag ccgcaatcga
      121 tgacggcggg cgaagaactt acgtgcccct gcccggcacc gaggatgtcc acgcccagcg
      181 cgtacctgac cgtggtgtag gtgaggatcg cctggccgtt gccgtcgcga acgccggtct
      241 cgaagaaagt gccggacggc ccgtacccgg agccaccgcc gccctcctgg tcattggtgc
      301 tgccgccgta atagccaccg ccaccaccgc cgcgcgtcgc ccccgaaccg aatcctcctc
      361 cgccgccgcc ctggccgagc gaccccggcc aggtgccggc tccgccttcg gtctgggtgc
      421 cgggggcacc ggcggtcggt gaggtgccgc tcgtgccgac gagtccgccg ccatcgccgc
      481 cgcgagaggc accgccgtaa tttccgacac cgccaccgcc accggccaca agcactctgt
      541 tgcccagtcc ggttccgccg atcctgaggt ctgtcccgcc gcccccgccg gcaaatccgt
      601 gaccgccccc gttccagcca ccggcaagaa agccgccggt tccgccgatg tacagacgca
      661 tgagctggcc cgggctgacg tcgaaggtcg ccttcgcgcg accgcccttg ccaccgacga
      721 agccgctgtt gccgttgttg cttcccgccg cttcgtagag atcgaactcg accgagtgca
      781 cgccgagcgg gacagtccag gcctgcgggc cacctgtgta gccgatcgtc tcggtgtcgg
      841 cggtggccaa agtcggggac ccggcacaca agagagcggc gatcaacccc acaagtccga
      901 gccgccatcc cacccgcagt agaagtgcgc ttctaaccgg tcggctcata tggagacacc
      961 ctatccggag tgttctgtac aaattggatt cggaactgta caatttcagc atggccactt
     1021 tcagcatcag caaagcgagg gagtcacttt cggacgcggt cgagcggtcc cggaccgaag
     1081 ccgtctttct cgagcggcat ggcaagaagg ctgcggtgat ggtcagtccc gagcgctacg
     1141 aagagatgtt ggaagcgttt gaggagatcg aagatgtcgc tgcctttgat caggcgatgg
     1201 ccgaagaaag tccgaacatc ccctgggacg aagccaaggc cgacctgggt tgggattgag
     1261 ccgctatcgg atagagctgc gcccggccgc gctccggtcc ctgcgcaaac tagaccccca
     1321 gatcagacgg agagtccaag gagcgatcgc tctccttgcc gacgaccctc gcccgccagc
     1381 taccaggaaa ctgaaaggac gagacgcctt ccgggtccgg gtgggcgact accgggtgat
     1441 ctacaccatt gaagacgagg tgctgttgat cgttgtggtt acgctcgggc accggcggga
     1501 cgtctacgag cggtagatac tgccgctgtt gccgtcgcct tagagggcga gccaggggcg
     1561 tgctgggccg gacgcctcac tccgcgaagc ggttggtctt ttgtgcccga gcgggaacca
     1621 cgcagggtga aagccgctgg gatccggcct gccgcgcctg ggcgatccat accccggggt
     1681 cgtactcgta aacgactgcc agggattcgc gtgcctatac ttgcggcggt ccggggggtc
     1741 cagtcgaacg agcagggaga agtgccatga gtgtgaagtt ccgcggtttc ctcggattgc
     1801 tgcttgccgc agtcgtcctt tcaaccggtc acgccgacgc ggcgaagaac gggccgatcc
     1861 tgttcagcga ctacacctcg atctggaaga tcaaggcaga cggttcgggc ctcaagcggg
     1921 tggcgaagaa gaaggcctgg agtcttcacg cctctcccaa cggcaaacag ctcctctaca
     1981 cacatgacgg cctcttccgg atgccgataa acggcggcag atcggtcaac ctgctcaagc
     2041 gctacccgat cgtcgaccgg ttcgccggtg tcagatgggc ttcctggtcg ccgaacggca
     2101 agcagatcgc tttcgagggc ggcaacgacg gccgcatcta tctgatcaag tcaaacggcc
     2161 gcggcctgag gtacctgttc cgcaagtacc ggaccggcct gctccacccc gtctggtccc
     2221 cgaacgggaa ggaaatcgcc tacatcgacg tccacaacaa ctcctcgctc atggcggtga
     2281 acctcaagtc gggtaagaag cgaaccatct acccgggcac cggtga
//
LOCUS       DAOQMF010000274         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29510_length_2319_cov_3.761926, whole genome shotgun sequence.
ACCESSION   DAOQMF010000274 DAOQMF010000000
VERSION     DAOQMF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29510_length_2319_cov_3.761926"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="PLV77_05430"
     CDS             <1..1308
                     /locus_tag="PLV77_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC15321.1"
                     /translation="RDNDSGEQYEVTADYVIAADGGRTVPGLLGIEYEGLGTVSETAT
                     LHVSADFSDLAIDDDVLIRWTISPVSGAGVVMVPMGPEKWGPESEEWVIHIQYLVGDP
                     REITDEEVLADVRSAMEIGELPMEIHKISRWTVEAVLASSFRSGRVFLVGDCAHKHPP
                     TGGLGLTSAIHDVHNLTWKIASVLKGEAGESLLETYEPERRPVVARNAQRSLENAINH
                     LGTMAEIGVTPENSTEENWAAMKRLWNEDPADQEFREKVARGARSQSMEFNELNVEYG
                     YAYDSAAVVADGSPPAPNPDEVRIYLPSTRPGSPLPHAWLDDLEGNRQPIKDLVRPGR
                     FLLIAGEEGDEWLQAANDLAGSAKVPLDSLRIGHIDGDLFDPQLTWIRNRGIEPDGAI
                     LVRPDRFVAWRSMSSASNPGQTLAKALTSVLGREVHMPEGAAA"
     gene            1305..2189
                     /locus_tag="PLV77_05435"
     CDS             1305..2189
                     /locus_tag="PLV77_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC15322.1"
                     /translation="MSAVSKIGHMAMRVSDLDRAVEFQSEVMGLVETERQGGTSFMTC
                     NDRHHELILIQSGKRGYDHIGLEVATAEDLEAVRAQAAAAGGDVLTQIQEGETGIDRC
                     FFVRSPAGHVFKIFLGMEKVAEPEPGDRPSQFEHVSTKALRMGREERFLSEGLGFRFS
                     DRLGRTASWWHCDPDHHGMAVVIAPRAELSHYAWSLEDLNALGRTADRLRARGQRAIW
                     GPSRHGPGNNLFLYFHDADGAMIECCAELEKMPPEGDYKPRKWTGGMKDINQWGSTPP
                     AKFLLTGFPINHREGEVK"
BASE COUNT          452 a          727 c          767 g          373 t
ORIGIN      
        1 agggacaacg attccggtga gcagtacgaa gtgaccgccg actacgtgat cgcggccgac
       61 ggcggccgca cggtgccggg ccttctgggg atcgaatacg agggtctcgg cacggtttcc
      121 gagaccgcga cccttcacgt ctcggccgac ttctccgacc tggcgatcga cgacgacgtg
      181 ctgattcgct ggacgatctc cccggtgagc ggagccggtg tggtcatggt tccgatgggg
      241 ccggagaaat ggggtccgga atccgaggaa tgggtgatcc acatccagta cctggtcggg
      301 gacccgcgtg agatcaccga cgaagaggtc ctggccgacg tgcgaagcgc gatggagatc
      361 ggcgagctgc cgatggagat ccacaagatc tcgcgctgga ccgtcgaagc ggtcctcgcc
      421 tcctcgtttc gctccggtcg ggtgttcctg gtcggtgact gtgcccacaa gcaccctccg
      481 accggcggcc ttggcctgac cagcgcgatc cacgacgtcc acaacctgac ctggaagatc
      541 gcgtcggtgc tcaagggcga agccggcgag agcctgcttg agacctacga gccggaaagg
      601 cgaccggtcg tcgcccgcaa cgcccagcgc tcactcgaaa atgcgatcaa ccatctcggc
      661 acgatggccg agatcggagt gacccccgag aactcgaccg aagaaaactg ggcggcgatg
      721 aagcgactct ggaacgagga tcccgccgac caggagttcc gtgagaaggt ggcgagggga
      781 gcgcgctcgc agtcgatgga attcaatgaa ctgaacgtcg aatacgggta cgcctacgac
      841 tcggcagcgg tggttgcgga cggaagtccg ccggcaccga atcccgatga ggtgcggata
      901 tatttgcctt caacccgtcc cggttcaccc ctgccgcatg cctggctgga tgacctggaa
      961 ggcaaccgtc agccgatcaa ggacctcgtc agaccaggcc gtttcctttt gatcgccggc
     1021 gaagaaggcg acgaatggct tcaagccgcg aatgacctgg ccggctcggc caaagtcccg
     1081 ctggacagcc tgcggatcgg acacatcgac ggtgacctct tcgacccgca gttgacctgg
     1141 atccgcaacc gggggatcga gcctgacgga gcgatcctcg tccggcccga ccgtttcgtg
     1201 gcctggcgta gcatgagctc ggccagcaac ccggggcaga ccctggccaa ggccctgacc
     1261 tcggtacttg gccgggaagt ccacatgccg gaaggagcag cagcatgagt gccgtttcca
     1321 aaatcggtca catggcgatg cgggtaagtg accttgaccg tgccgtcgag ttccagagcg
     1381 aagtgatggg tctggtcgaa accgagcggc agggcggcac ctcgttcatg acctgcaacg
     1441 accgtcatca cgagctgatc ctgatccagt ccggcaagcg gggctacgac cacatcggcc
     1501 tcgaggtcgc gactgccgaa gatcttgagg cggtccgcgc ccaggctgcc gcggccgggg
     1561 gagatgttct cacccaaatt caggagggcg agaccggcat tgaccgctgt ttcttcgtcc
     1621 gctcaccggc cggccatgtc ttcaagatct tcctcggcat ggagaaggtg gccgagccgg
     1681 agccgggtga tcgcccctcg cagttcgagc atgtttcaac caaggcgcta cggatgggcc
     1741 gggaggagag gttcctttcg gaaggcctcg gattcaggtt ctccgaccgg ctgggccgca
     1801 ctgccagctg gtggcactgc gaccccgacc accacggcat ggcggtcgtg atcgcacccc
     1861 gggcagagct ttcccactac gcctggagcc tggaagacct gaacgccctc gggcgcaccg
     1921 ccgaccggct ccgggccagg ggtcagcggg cgatctgggg accgagccgg cacggtccgg
     1981 gcaacaacct cttcctctat ttccacgacg ccgacggggc gatgatcgag tgctgcgccg
     2041 aactcgagaa gatgccgccc gaaggtgact acaagccccg caagtggacc ggcggcatga
     2101 aggacatcaa ccagtgggga tcgaccccgc cggcgaagtt ccttttgacc ggattcccga
     2161 tcaaccaccg ggaaggggaa gtcaagtgaa gctgcgctct ggcccggaag ggctgatggt
     2221 cgaagccgga ccgggcgagt gggtctcgct tcccgcgctg aagagagaag cgaccgaact
     2281 cgatctggac gacgcgcaga cgacggacat gatcgcctt
//
LOCUS       DAOQMF010000275         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29521_length_2318_cov_4.019001, whole genome shotgun sequence.
ACCESSION   DAOQMF010000275 DAOQMF010000000
VERSION     DAOQMF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29521_length_2318_cov_4.019001"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PLV77_05440"
     CDS             complement(<1..1255)
                     /locus_tag="PLV77_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNC15323.1"
                     /translation="MAALIAGLVLLGATTTVANAGSKQLKKAPKITSKADYTARGSVG
                     DAYVKDADPGQRLLLVNENNRIVRSGKADRFGSKIFYDVAPDRIFTVRTPTANGSQGS
                     RRFRVLKPGQNPKQSWYDAKPDLKAGLNYVTMRDGTELAVTVRLPYGKTSLSQGPFPT
                     YVEYSGYQTAAPHDALLGLVAGLPDSSLPEPLGDLLDTITGSLPGSPVPDPDLTPASS
                     TAVGSLIGPLLDFATVSVQMRGSGCSGGAFDLFGWPTTYDGYDAIETIAAQDWVKGGK
                     VSMGGISFSGITQLFVAGTRPPHLSAVSPLSTTDDVYYGTGFPGGIFNNGFAYSWITE
                     RGEDAEPAPEGGQPWAKIMTTTGDPDVAEPLRSEQKQHCIDNQTLRLQTLNHNAKIEN
                     NPFRTPGLFAKRSPGAWVKNINVPIF"
     gene            complement(1540..>2318)
                     /locus_tag="PLV77_05445"
     CDS             complement(1540..>2318)
                     /locus_tag="PLV77_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNC15324.1"
                     /translation="WEADFNSWPIRTTRPTAFYLGKNGTLSTSKSKTGSSVSYLSDPS
                     ARPAKTLGENAQGDSWLAQPPYNWQPVADGHGLGFITPALTKDVFMAGPSSVDLYLKS
                     SKPNTDIQVTLTEVRPDGNETYIQNGWLRASHRAINSKLSTAYNPVQTWLKSDQAPLK
                     KGKYNYARIQIFPAAHVFRAGSKIRLNIQAPGGDRTIWDFDTIEKGNTTNTIGLGGVI
                     PSKLVLPVIPGEDAQGTELPGPTELRGEPNRTYEPASNGG"
BASE COUNT          349 a          686 c          829 g          454 t
ORIGIN      
        1 agaagatcgg cacgttgatg ttcttcaccc acgccccggg cgagcgcttg gcgaacaggc
       61 caggggtacg gaaggggttg ttctcgatct tcgcgttgtg attgagcgtc tgcaggcgca
      121 gcgtctggtt gtcgatgcag tgctgcttct gctcgctgcg aaggggctcg gccacgtccg
      181 ggtcaccggt cgtggtcatg atcttcgccc agggctgacc gccctccggg gccggctcgg
      241 cgtcttcacc gcgctcggta atccagctgt aggcgaagcc gttgttgaag atgccgcccg
      301 ggaaaccggt gccgtagtag acgtcatcgg tggtcgagag gggtgagacc gcggaaagat
      361 ggggcggccg cgtgccggcc acgaagagct gggtgatccc cgagaaggag atgccgccca
      421 tgctgacctt gccgcccttt acccagtcct gggccgcaat ggtttcgatc gcgtcgtatc
      481 cgtcgtaggt ggtcggccag ccgaacaggt cgaatgcgcc gccggaacag ccggaaccgc
      541 gcatctggac gctgaccgtg gcgaagtcga gcagcgggcc gatcagtgag ccgaccgcgg
      601 tcgagctggc cggggtcagg tccggatcgg ggaccgggct gccgggcagg ctgccggtaa
      661 tcgtgtcgag caggtcaccc agcggttcgg gcaggctgct gtcaggcagg ccggcgacca
      721 ggccgagcag cgcatcatgg ggagcggcgg tctggtagcc ggagtactcg acgtaggtcg
      781 ggaagggacc ctgagagagg gaagtcttgc cgtaaggcag gcgtacggtg accgcgagtt
      841 cggtgccgtc gcgcatcgtc acgtagttga gaccggcctt gaggtccggt ttcgcgtcgt
      901 accaggactg cttcggattc tgtccgggct tgagaaccct gaaccgtctc gagccctgcg
      961 agccgttggc ggtcggggtg cggaccgtga agatccggtc cggggcgacg tcatagaaga
     1021 tcttggagcc gaagcggtcg gctttgcccg agcggacgat ccggttgttc tcgttgacca
     1081 gcaacaggcg ctggcccggg tcggcgtcct tgacataggc gtcgccgacg cttccgcgtg
     1141 cggtgtaatc cgccttcgag gtgatcttcg gagccttctt cagctgtttg gagcccgcgt
     1201 tcgcaacggt cgtggtggcg ccgagcagga cgagtcctgc gatcagggct gccatggtgg
     1261 acagaaacac atgaatcctt cgcatcgttg cgaaacctcc cggttgtttt cggtcgcgga
     1321 ctccccgcgt gcctgtttcg agcaatggcc tttcattccc cgaaacccga tctcataaac
     1381 agcctgtttc ccaaggaagt cggttaacca aaaagggccg cccttttgga cggccctccg
     1441 ccggagccct gccatcaaat atttgatggc cctggccggg cgggttcccg ctgccgcccg
     1501 gccagggccg ggcggcgtgc aggtggagtg acggcggcgt cagccgccgt tactggcggg
     1561 ttcgtaggtg cggttgggct cgccgcggag ctcggtcggt ccgggcagct cggtgccctg
     1621 ggcgtcttcg cccgggatca ccggcaggac cagcttcgac ggaatcacac cgccgagacc
     1681 gatcgtgttg gtggtgttgc ccttttcgat cgtgtcgaag tcccagatcg tccggtcccc
     1741 accgggtgcc tggatgttca ggcggatctt cgagccggcc cggaacacgt gggcggccgg
     1801 gaagatctga atccgggcgt agttgtactt gcctttcttc agcggagcct gatcggactt
     1861 cagccaggtc tgcaccgggt tataggcggt tgagagcttg ctgttgatgg cccggtgcga
     1921 ggcccgcagc cagccgttct ggatgtaggt ctcgttgccg tccgggcgga cctcggtcag
     1981 ggtcacctgg atgtccgtgt tgggcttgct cgacttgagg tagagatcaa cgctggacgg
     2041 tccggccatg aagacgtcct tggtcagggc cggcgtgatg aagccgagcc cgtggccgtc
     2101 ggcgaccggc tgccagttgt acggcggctg ggccagccag gagtcaccct gggcgttctc
     2161 gccgagggtc ttggccggac gggcgctcgg gtcggacagg tagctgaccg aagaaccggt
     2221 cttcgacttg ctggtgctca gggtgccgtt cttgcccagg tagaacgcgg tgggcctggt
     2281 cgtcctgatc ggccagctgt tgaagtcggc ctcccact
//
LOCUS       DAOQMF010000276         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29748_length_2309_cov_2.567879, whole genome shotgun sequence.
ACCESSION   DAOQMF010000276 DAOQMF010000000
VERSION     DAOQMF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29748_length_2309_cov_2.567879"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1831)
                     /locus_tag="PLV77_05450"
     CDS             complement(89..1831)
                     /locus_tag="PLV77_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15325.1"
                     /translation="MPDGKRLVTVVASPDLTAPQLAITRLDGSHPKCVTCGLDTAVVP
                     GAPVDAAFGKPAPFPDGKRLMVRLGLRPGAGGSPTQNFTYGVVECTPTLVNCEERTLS
                     PIIMPGGGIDQGTQNREGRLSPDGRWFGWTEFTMNGTAMSVGRLAKNGGQYDLENVRI
                     LNPSGPLGTDSAAWRAQSPYFELKNFTSDGKSVLYSTTYDASNFDTWRVDLRNGKRTR
                     ITRGIDWEEDVSTSPDGGSYLQFSSRGFDRMPIFALMPRPPFIEFALFSWVGRYELNQ
                     VNRKCLLEPWLLDSGGERGTYFGQPVDPRPGTGWDNRTLGQWSPDGTRLLMWQARGND
                     DGTPEKPGARIVIADLPARKATGKRSNNTTPVPGWAPARSSYTGFLANSGTFTVYGKK
                     TGSATVSMEGTAFGGSWSVTYDNYSDDGKSFLDGTESTTQASILSGITWTAELTLTGK
                     HSGHLSANLQIIKGEAGAGAQVSGTVSQSLDDRTVSEFPSATCEPLARPRLRVKAARQ
                     KAGRVRVLVTGKVTADPVWRPVRYATVRLGAERVRTDRKGIAVLKGKRGRMITASAAG
                     FTGAKARAPRPGRR"
BASE COUNT          383 a          736 c          769 g          421 t
ORIGIN      
        1 cgcggaacca ccgatctgat cgccgaggag gtcaggcagg gacgggttgc gacactgccg
       61 gacctgaccg gcgacctggt tgacgtgatc agcgtcgccc tgggcggggc gcccgtgcct
      121 tcgcgccggt aaacccggca gcacttgcgg taatcatccg gcctcgcttc cccttcagca
      181 cggcgatgcc cttccggtcg gttcgaaccc gctcggcacc aagccgcacc gtggcgtacc
      241 gcaccggccg ccagaccgga tcggccgtga ccttgccggt aaccagcacg cgaacccggc
      301 ctgccttttg acgggcagcc ttgacccgga gccggggcct tgccagcggc tcgcaggtgg
      361 cggaggggaa ttccgagacg gtgcggtcgt ccaggctctg gctcaccgtg ccggaaacct
      421 gggcaccagc gccggcctcc cccttgatga tctggagatt cgcgctcagg tggcccgaat
      481 gcttcccggt cagagtcagt tcggcggtcc aggtgattcc gctcaggatc gatgcctggg
      541 tggttgactc ggtgccgtcg aggaaagact tcccgtcatc ggaatagttg tcgtaggtaa
      601 cgctccagct gccgccgaaa gcggtgccct ccatgctgac ggtggccgat ccggtcttct
      661 tcccgtacac ggtgaaagtg cccgaattgg cgaggaaacc ggtgtaggag cttctggccg
      721 gcgcccatcc gggaaccggc gtggtgttgt tactcctctt ccctgtcgcc ttgcgggcgg
      781 gaaggtcggc gatcacgatt ctcgcccccg gtttctccgg ggtgccgtca tcgttgccgc
      841 gggcctgcca catgagcagc ctggttccgt ccgggctcca ctgccccagg gtgcggttgt
      901 cccagccggt acccgggcgg ggatcgaccg gctggccgaa gtaagtgccg cgttcgccac
      961 cggaatcgag cagccacggc tcgagcaggc acttccggtt cacctgattc agctcgtagc
     1021 gcccgaccca ggaaaagagc gcgaattcga tgaaaggcgg cctcggcatc aacgcgaaga
     1081 tcggcatgcg gtcaaacccg cgagagctga actggaggta tgagcccccg tcgggggagg
     1141 tggaaacgtc ctcttcccag tcgatgcccc tggtgatccg ggtccgtttg ccgttccgga
     1201 ggtcaactcg ccaggtatcg aagttgcttg cgtcataggt ggtggagtag aggaccgatt
     1261 tgccgtccga ggtgaagttc ttgagttcga agtagggaga ctgggcgcgc caggccgccg
     1321 agtccgtccc cagcggcccg gaaggattga ggatcctgac gttctcgagg tcgtactgac
     1381 ccccgttctt tgccagacgc ccgaccgaca tcgccgtgcc gttcatcgtg aactcggtcc
     1441 agccgaacca gcggccatcg ggcgaaaggc gcccctcgcg gttctgggtg ccctggtcta
     1501 ttccgccgcc gggcatgatg atcggggaaa gcgtccgctc ttcgcagttg acgagtgtcg
     1561 gagtgcactc cacgaccccg taggtgaaat tctgggttgg gctgcccccg gcgccaggcc
     1621 tcaaaccgag ccggaccatc agccgcttgc cgtcggggaa cggtgccggc ttgccgaagg
     1681 ccgcatccac cggggcaccg ggaacgacgg cggtgtcgag gccgcaggtg acgcacttcg
     1741 gatgtgaacc gtcgaggcgg gtgatcgcca gctggggagc ggtgaggtcg ggtgacgcga
     1801 cgaccgtcac gaggcgcttt ccgtccggca tccagtccgg ttccgattcg cccgagtacc
     1861 cggcgggaac ggggaggtgg tgcacggaga tcggctcaag gggtgcgggt gccgcgtctg
     1921 cagcctgcgt gcaagcaaac aacgacagga gaaccaacag ccctgctgct cctgcggaca
     1981 ccatcgaacc gatgtgcttc atgtggcccc tcctcaaggg tcatttccca tgacggaaac
     2041 cccgccgaca cgatcatagc gtacgcatgc gtactttttc aggtggctgg aagggcctgc
     2101 ttcgggacac cgtttgccgg ggttggggcc gatccgggaa aaatcgcaat ccgatgccaa
     2161 atttcgggta gtctgtttgc cggagttatt tctccccagg cattcgaggg agtctcttgc
     2221 cgcagtacgg acgtcgtttc gatcacccga cgatgtcggg aaaccggtcc acaaacccgg
     2281 accgtgggcg ccgctcggcc gttctgacc
//
LOCUS       DAOQMF010000277         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29786_length_2307_cov_3.866341, whole genome shotgun sequence.
ACCESSION   DAOQMF010000277 DAOQMF010000000
VERSION     DAOQMF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29786_length_2307_cov_3.866341"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /gene="thrB"
                     /locus_tag="PLV77_05455"
     CDS             <1..827
                     /gene="thrB"
                     /locus_tag="PLV77_05455"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNC15326.1"
                     /translation="GPGYDVMAVALDLHLELEVKETGTFSVSCDALDVPLDRSNLVVR
                     AFETLQPADGISFRVGGEIPLARGMGSSAAAIVAGLVAADHLFELGLERQDLLERATA
                     LEGHPDNVAAAICGGFVICTGGPRLTATVIEAPEGLEGILVIPEVEVSTRKAREAIPE
                     QIPIKDAVANTASAAHLALGLARGDSDLIGMGLHDRLHQERRRHLFPKSMEIVEVAGE
                     IGAIGATISGAGPTVLVWTNWQETGGVVEQLRERCEGWAEVRRVGFSPLGADVPEL"
     gene            complement(953..1141)
                     /locus_tag="PLV77_05460"
     CDS             complement(953..1141)
                     /locus_tag="PLV77_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15327.1"
                     /translation="MKLSTAARSALTFIVVMIGLAIVGAIAGINVGPVEFVIWLVVLV
                     IGLALIVTGTNRSKQIEN"
     gene            complement(1146..1946)
                     /locus_tag="PLV77_05465"
     CDS             complement(1146..1946)
                     /locus_tag="PLV77_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC15328.1"
                     /translation="MSTTIAILSQKGGTGKTTLTRSLSEGLRRAGLSVLAIDADPQGN
                     LSEYFGIPADAFPTLGEVLAGRERAVDAIHTGPGGGEVIPANLGLAEAELMLAGKIGR
                     ELTLRNALREPKRRFDVILIDCPPTLGLLTVNALVASDKAILSTQAEHFSEQGAEQAM
                     EVIGLARENLNPELELLGLLLNIADMRTNHARSTLETMREQFGDLVFDTVIRRSIVYP
                     ESARAAKPIFDYRKRKSLDYAALTVEVLERLDYPAALAEAKSALAELP"
     gene            2006..>2307
                     /locus_tag="PLV77_05470"
     CDS             2006..>2307
                     /locus_tag="PLV77_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15329.1"
                     /translation="MGPVFTEVDIDAPREEIFDYLLDFDTRPFLYGDSVENFRLLRLD
                     SRGIGAGARWQFKRKKAWADSAITATEAPRRISERGSTGSYNRNVTLFSLGCEDEDP"
BASE COUNT          389 a          761 c          765 g          392 t
ORIGIN      
        1 tcggaccggg ctacgacgtg atggcggtgg ctttggatct tcacctcgaa ctcgaggtga
       61 aggagacggg gaccttctcg gtcagctgcg acgcgctcga cgtgccgctc gatcgcagca
      121 accttgtggt ccgcgccttc gaaaccctgc agcccgcgga cggcatttcc ttccgggtcg
      181 gcggcgagat ccccctcgcc cgcggcatgg gatcgagcgc cgccgcgatc gtcgccggac
      241 tggtcgcggc cgaccacctg tttgaactcg ggctcgaacg gcaggatctg cttgaacgcg
      301 ccacggctct cgagggccat cccgacaacg tcgccgccgc gatctgcggg ggcttcgtga
      361 tctgcaccgg cggcccgcga ctgaccgcaa ccgtgatcga agcgccggaa ggcctggagg
      421 ggatcctggt catcccggag gtcgaggttt ccacccgcaa ggcccgcgag gcgatcccgg
      481 aacagatccc gatcaaggac gcggtcgcca acacggcctc cgccgcccac cttgccctgg
      541 gcctggccag gggtgactcc gacctgatcg gaatggggct ccacgaccgg ctccaccagg
      601 aacggcgccg ccacctcttc ccgaaatcga tggagatcgt cgaagtggcc ggcgagatcg
      661 gggcgatcgg cgcgacgatc tccggggccg gacccaccgt cctggtctgg accaactggc
      721 aggaaaccgg gggagtggtc gagcagttga gggagcgttg cgaaggctgg gccgaggtaa
      781 ggcgagtcgg cttcagcccc ctcggcgccg acgtccccga gctctgagat ggacctgctc
      841 gtccggatcg gcctgatcaa ctaaccccag tcgggccggt cttcgccggc cctcccatcg
      901 aaacccgccc ctcccactcg cagagacgaa tggttgggag ggactccgtt tgtcagttct
      961 cgatctgctt ggaccggttt gtgccggtca cgatcagtgc caagccgatc acgagcacga
     1021 ccaaccagat gacgaactca acgggtccca cgtttatgcc tgcgatcgct ccgacgatcg
     1081 ccaggccgat cattacgacg atgaaggtaa gtgcgcttcg tgcggcagtc gagagtttca
     1141 tgcgatcagg gtagctcggc cagggcggac ttcgcctcgg ccagcgctgc cgggtagtcg
     1201 agccgctcga ggacttcgac cgtgagggcc gcgtagtcga gcgactttcg cttgcggtaa
     1261 tcgaagatcg gcttggctgc ccgggctgat tcgggataga cgatcgaacg ccggattacc
     1321 gtgtcaaaga cgaggtcccc gaattgctcg cgcatggtct ccagggtgct gcgggcgtga
     1381 ttggtccgca tgtcggcgat gttgagcagc agcccgagca actcgagctc cggattcagg
     1441 ttctcccggg ccaggccgat cacttccatc gcctgctcgg ccccctgctc ggagaaatgc
     1501 tcggcctggg tcgagaggat cgccttgtcg gacgcgacca gggcgttcac ggtgagcagg
     1561 cccagggtcg gcgggcagtc gatcaggatc acgtcaaaac gccgcttcgg ctcccgcagg
     1621 gcgttacgga gggtgagctc gcgcccgatc ttgccggcca gcatcagctc ggcctcggct
     1681 aggccgaggt tggcggggat cacctcgccg ccgccggggc cggtgtgaat cgcatcgacc
     1741 gcccgttccc ggccggccag cacttcaccg agggtgggga aggcatcggc cgggatcccg
     1801 aagtactcgg aaaggtttcc ctgggggtcg gcatcgatcg cgagaacgga aagtcccgcc
     1861 cggcgcaggc cctcggagag cgaccgcgtc aacgtggtct tcccggttcc tcccttctgg
     1921 gaaaggatcg cgatcgtcgt ggacaccggc acaccctatc gccggtcggg cacgcatcgg
     1981 cacgcaggtc gtagaatccg gatcgatggg tcccgtattc actgaagtcg acatcgacgc
     2041 gccgcgcgaa gagatcttcg actacctcct ggacttcgac acccgcccgt ttctttacgg
     2101 cgactcggtc gagaactttc ggctcctgag gctggattcg cggggcatcg gcgctggcgc
     2161 ccgctggcag ttcaagcgca agaaggcctg ggccgattcg gcgatcaccg cgaccgaagc
     2221 cccccggcgg atttccgaac gcgggtccac cggcagctac aaccgcaacg tgaccttgtt
     2281 ttcgctcggc tgtgaagacg aggaccc
//
LOCUS       DAOQMF010000278         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29872_length_2304_cov_2.630947, whole genome shotgun sequence.
ACCESSION   DAOQMF010000278 DAOQMF010000000
VERSION     DAOQMF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29872_length_2304_cov_2.630947"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /gene="priA"
                     /locus_tag="PLV77_05475"
     CDS             <1..995
                     /gene="priA"
                     /locus_tag="PLV77_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNC15330.1"
                     /translation="DPRQGPIHRRTWEALAEVREKGEKAIVMINRRGFAPWLTCQTCG
                     HHWGCPNCDVSLIVHRESDQLICHHCNHAEPLPRRCPDCGGTTLSQTGAGTQRIERLL
                     AEELAPMPVFRLDADTSAGPGGHATILSAFDQEESAILVGTQMVAKGHDFPEVTLAAI
                     LDADATLRFPDFRAEERTFSLVTQLAGRSGRSRTGGKVIVQTLAPGSPSISHAATHDS
                     AGFLAGELDRRRELGYPPFSHLIRIQLAAEAEPEVERAANLVAARLDDALPAGAEMLG
                     PAPMFRARNRYRRRLLVRSTTRAESIAAVHSTIEELLADKSLRKITLAIDVDPQ"
     gene            1079..1786
                     /gene="def"
                     /locus_tag="PLV77_05480"
     CDS             1079..1786
                     /gene="def"
                     /locus_tag="PLV77_05480"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC15331.1"
                     /translation="MASEDETATLSDEAAAYAEGMERAEEALAEAGADGAAGEEVEVE
                     PEGGEEPVRHYDEETLRRREAALARIVQYGDPMLKSRSSEVTDFDQALTDEIERMFEL
                     MKDGLGVGLAAPQAGKLRRLLVFQTNADAEPQELVNPDIEWLSDETEVAAEGCLSIPR
                     VIVEVDRPLYARVRGQDRNGAEVLIEASGHEARVLQHEIDHLDGILILDRTEKKQRKG
                     AMKALRRGESYSPHDDD"
     gene            1792..>2304
                     /locus_tag="PLV77_05485"
     CDS             1792..>2304
                     /locus_tag="PLV77_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNC15332.1"
                     /translation="MAGVFLGTSEFAGTVLDILAASDQRPELVVTLPDRRRGRGRREQ
                     ASPVADKAAALGIEVLKSGNVNEGEDLGRILEVGGEWASICAFGQLIKEPLLTELPML
                     NVHPSLLPRWRGAAPVERAIMEGDEVTGVCVMRLVAGLDSGPVAMREVTAIGQDEDFG
                     SLSARLAEIGG"
BASE COUNT          398 a          769 c          795 g          342 t
ORIGIN      
        1 cggaccctcg gcagggtccg atccaccgcc gcacctggga agccctggcc gaggtgaggg
       61 agaagggcga gaaggcgatc gtgatgatca accgccgggg ctttgcgccc tggcttacct
      121 gccagacctg cgggcaccat tggggctgcc cgaactgtga tgtctcgctg atcgtccatc
      181 gggagtcgga ccagctgatc tgccaccact gcaatcacgc cgaaccgctg ccccggcgct
      241 gccccgactg cggcgggacg acgctctcgc agaccggggc cgggacccag cggatcgaac
      301 ggctgctggc cgaggagctg gctccgatgc cggtctttcg ccttgacgcc gacaccagcg
      361 ccggtccggg tggccacgcg acgatcctca gtgccttcga ccaggaggag agtgcgattc
      421 tggtcggcac ccagatggtc gccaaggggc acgacttccc cgaagtgacc ctcgccgcga
      481 tcctcgacgc cgatgccacc ctccgcttcc ccgacttccg ggccgaggag agaacctttt
      541 cgctggtcac ccagctggcc ggccgcagcg gccgcagccg gaccgggggg aaggtgatcg
      601 ttcagacgct ggctccgggc agcccctcga taagccacgc cgcgacccat gattccgccg
      661 gctttctggc cggcgagctc gatcgacgcc gcgaactggg ctatccgccg ttttcccatc
      721 tgatccgcat ccagctcgcg gccgaagccg agccggaagt ggagcgggcc gcgaacctgg
      781 ttgccgcccg cctcgatgac gccctccccg ccggagcgga aatgctcggc cccgccccga
      841 tgttccgggc acgaaaccgc taccgccgcc gcctcctggt ccgctcaacg accagagccg
      901 aatcgatcgc ggcagtccac agcacaatcg aggaactgct cgccgacaaa tccctgagga
      961 agatcacgct ggcaatcgac gtcgatcccc agtgatcagg cgcagcaggc aggctcgccc
     1021 cttgacgggg gtttccacgg ctcctgcgtg attccgggcg tcggtagact cggcgacgat
     1081 ggcttccgaa gatgaaaccg ccaccctgag cgacgaagcg gccgcatatg ccgagggcat
     1141 ggagcgggcc gaagaggcgc tggccgaggc cggcgccgat ggggctgccg gggaagaagt
     1201 ggaagtcgag ccggagggcg gcgaagagcc ggtccgtcac tacgacgaag agacgcttcg
     1261 ccggcgcgag gctgcgctgg cccgaatcgt ccagtacggc gacccgatgc tgaagagccg
     1321 ctcctcggag gtgaccgact tcgaccaggc cctgaccgac gagatcgagc ggatgttcga
     1381 gttgatgaag gacggtctcg gggtcggact ggcggcaccg caggccggca agctgcgccg
     1441 gttgctcgtc ttccagacca atgccgacgc ggagccgcag gaactcgtca accccgacat
     1501 cgaatggctt tccgacgaga ccgaggttgc ggccgaaggt tgcctgagca ttccccgggt
     1561 gatcgtcgag gtggaccgcc cgctgtatgc cagggtccgt ggccaggacc ggaacggcgc
     1621 cgaagtgctg atcgaggctt ccggacacga ggcccgggtg ctgcagcacg aaatcgatca
     1681 cctcgacggc atcctgatcc tcgatcgcac cgagaagaag cagcgcaagg gcgcgatgaa
     1741 ggccctgcgg cggggcgaga gctactcgcc ccacgacgac gactgaagca aatggccggc
     1801 gtctttctcg gtacctctga attcgcagga acggtgctgg acatcctggc tgcctcggac
     1861 caacggccgg agcttgtggt caccctgccg gaccggcgtc gcggcagggg gcgccgggaa
     1921 caggcttctc ccgtcgccga caaggccgca gccctcggca tcgaagtcct gaagagcggc
     1981 aacgtcaacg agggtgaaga cctcgggcga atcctcgaag tcggtggcga gtgggcgtcg
     2041 atctgtgcct tcggccagct gatcaaggag ccgctgctga ccgagttgcc gatgctgaac
     2101 gtccatcctt cgctgcttcc ccgctggcgg ggggcggccc cggtcgaacg ggcgatcatg
     2161 gagggcgacg aggtgaccgg ggtctgtgtg atgcgcctgg tcgccggcct cgattcgggg
     2221 ccggtggcga tgcgggaagt cacggcgatc ggccaggacg aagatttcgg ttccctctcg
     2281 gcccggctcg cggagatcgg cggg
//
LOCUS       DAOQMF010000279         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29911_length_2302_cov_3.789942, whole genome shotgun sequence.
ACCESSION   DAOQMF010000279 DAOQMF010000000
VERSION     DAOQMF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29911_length_2302_cov_3.789942"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLV77_05490"
     CDS             <1..762
                     /locus_tag="PLV77_05490"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15333.1"
                     /translation="LTSIFEPLTLNDTIRVTEGGPAALVDQVICPGVPGENLVSQVIR
                     MMRSEGWDPPLLKVEIEKRIPVQAGLGGGSADAAAMLRLGVGEVEDLAGLALLLGADV
                     PSQLNPKPLLVEGVGQKLTTLPAPAEHWVVLLPFERGLSTRAVFDEADRMKLGRSQEE
                     IDDISGRLWAVATNAVSPLAYPALLINDLEPAAISLKPEIGLGKERLEAAGAGFTGMT
                     GTGPTVFGLFPDRESAEAAAAAVGPEALVCQGGVV"
     gene            773..2248
                     /locus_tag="PLV77_05495"
     CDS             773..2248
                     /locus_tag="PLV77_05495"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HNC15334.1"
                     /translation="MIKVPETKKGKRLTLAGIAAAALVIFILYREFFPELDLQQLLDD
                     FANFLGAWTYLVVGLLAFLETGAFVGLIVPGETAMLIGGAVAGQGVINVFLLIAIAWL
                     MAFLGDSTSFWLGHRLGREFILKHGPKFGISAERYEQVEDYFEKHGGKTVLMGRFIGL
                     VRALSPFVAGSSGMRYRAFAPYSILGSGLQITLHILAGYFFARSIEAAAEYVGLAALV
                     IGTMIVVSVASYLAWKYLREPNNRARAVEWMEGRWYSAWLVRLARRYETQLRWIQDRF
                     TPGGTFGLEVTTLFAIIAVASFVLFAYAGILLDDGGPTPGDLRAFDFRDLIETGWLTS
                     IAKVVTVLGSQVVLLPLITVTAVILAVRQHWSEMLVLVLSSIAIAFGIDWIKDWVARP
                     RPDGGMIEVAGYAYPSGHAAHSIFYVWLSVTIAVRLRPDMARKAALITGGILLAALVG
                     LSRVYLGVHYLSDVFGGWALGALCFSFFAVWALLLGQLRKN"
BASE COUNT          332 a          778 c          751 g          441 t
ORIGIN      
        1 ctcacctcga tcttcgagcc gctgaccctc aacgacacga tccgggtaac cgaaggaggc
       61 ccggcggccc tggtcgacca ggtgatctgc ccgggcgtgc cgggcgagaa cctggtcagt
      121 caggtgatca ggatgatgcg ctccgagggc tgggatcccc ctctgctcaa ggtcgagatc
      181 gaaaagcgga tcccggtcca ggccgggctc ggcggcggca gcgccgatgc cgcggcaatg
      241 ctccgcctcg gggttggaga ggtcgaggac ctggccgggc tcgccctgct gctcggcgcc
      301 gatgtgcctt cgcagctcaa tccgaagccc ctgctggtcg agggggtcgg gcagaagctg
      361 accaccctcc cggcgccggc cgaacactgg gtcgttctgc tgcctttcga gcgaggactt
      421 tcgacccggg cggtgttcga tgaagccgac cggatgaagc tcggccggag ccaggaagag
      481 atcgacgaca tttccgggcg cctctgggcg gttgccacca acgcggtgtc cccgctcgcc
      541 tacccggccc tgctgatcaa cgacctcgag ccggccgcga tttccctcaa gccggaaatc
      601 gggctcggca aggagcggct ggaggcggcc ggggcgggat tcaccgggat gaccggcacc
      661 ggccccaccg tcttcgggct tttccccgac cgggaatcgg cagaggcggc ggccgccgcc
      721 gttggtccgg aggcgctcgt ctgccagggc ggcgtggtct gatttcgcgc tcatgatcaa
      781 ggtcccggag acgaaaaagg gcaaacggct gaccctcgcc ggcatcgcgg cggcggccct
      841 cgtcatcttc atcctctacc gggagttctt tcccgagctc gacctccagc agctgctgga
      901 cgacttcgcg aacttcctcg gtgcctggac ctacctcgtc gtcggcctgc tcgccttcct
      961 tgaaaccggc gccttcgtcg gcctgatcgt ccccggagaa acggcgatgc tgatcggcgg
     1021 tgcggtcgcc ggtcaggggg tgatcaacgt cttcctgctg atcgcgattg cctggctgat
     1081 ggccttcctc ggtgattcga ccagcttctg gctcggccac cggcttggcc gcgaattcat
     1141 cctcaaacac ggcccgaagt tcgggatctc ggcggaacgc tacgaacagg tcgaggacta
     1201 cttcgaaaag catggcggca agaccgtcct gatgggccgg ttcatcgggc tggtccgggc
     1261 cctttccccg tttgtggcgg gcagctccgg catgcggtac cgggccttcg cgccctacag
     1321 catcctcggc tccggcctcc agatcaccct gcacatcctg gccggctatt tcttcgcccg
     1381 cagcatcgaa gcggcggccg agtacgtcgg gctggcggcg ctcgtcatcg gcacgatgat
     1441 cgtggtctcg gtcgcctcct atctcgcctg gaaatacctg cgcgaaccga ataaccgggc
     1501 cagggcggtc gagtggatgg aaggccgctg gtacagcgcc tggctggtgc gactggcccg
     1561 tcgctacgag acgcagttgc gctggatcca ggaccgcttc acgccgggcg ggactttcgg
     1621 tctcgaggtg acgacgctct tcgcgatcat cgccgtggcc tcattcgtgc tcttcgccta
     1681 cgccgggatt ctgctcgacg acggtggccc gacccccggc gacctgcggg ccttcgattt
     1741 cagggacctg atcgagaccg gctggctgac ctccatcgcc aaggtagtca cggtgctcgg
     1801 ctcccaggtg gtgctgctgc ccctgatcac cgtgaccgcg gtgatactgg cggtccggca
     1861 gcactggtcg gaaatgctgg tcctggtcct ttcctcgatt gcgatcgcat tcggaatcga
     1921 ctggataaag gactgggtcg cccggccccg ccccgatggc gggatgatcg aggtcgccgg
     1981 ctacgcctac ccctccggac acgccgccca ttcgatcttc tatgtctggc tctcggtcac
     2041 catcgcggtc cggcttcgcc ccgacatggc ccgcaaggct gccctgatca ccgggggcat
     2101 tctgctggcc gcgctggtcg gcctgagccg ggtctatctc ggtgtccact acctgagcga
     2161 cgtcttcgga ggctgggctc tcggggcact ctgcttctcg tttttcgcgg tctgggccct
     2221 gcttctgggc cagttgcgca aaaattaggc cgatggtgct cgccgtttca acccagatct
     2281 ggtatttcgg cgccgccgga ct
//
LOCUS       DAOQMF010000280         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_29977_length_2299_cov_4.361854, whole genome shotgun sequence.
ACCESSION   DAOQMF010000280 DAOQMF010000000
VERSION     DAOQMF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29977_length_2299_cov_4.361854"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLV77_05500"
     CDS             <1..1082
                     /locus_tag="PLV77_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNC15335.1"
                     /translation="RMAALEGGVAALALASGQAAVTYSVLNVCRTGDNIVALSTLYGG
                     TYNLFAHTLPQYGIEVRFVDPDKPEELEKHVDDKTRLVFGETIGNPRLNVMDVEAWAN
                     AAHAQGLPLVVDNTVATPVLTRVFELGADIAVHSATKFIGGHGTTIGGVIVDSGNFDW
                     VANADRFPGLTQPDPSYHGVVWADALGPAAYIGRVRTVLLRNTGAALSPFNAFLLLQG
                     VETLPLRMERHSANALAVAEHLSDSENVLWVNYPGLPGFSHHETASKVLTGGYGALVT
                     FGIKGGRDAGRKFIDSLELFSHLANIGDAKSLAIHPATTTHSQLNDEELEDSGVTADT
                     VRLSVGIETIGDILADIDQALAKAS"
     gene            1083..2276
                     /locus_tag="PLV77_05505"
     CDS             1083..2276
                     /locus_tag="PLV77_05505"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNC15336.1"
                     /translation="MPDASSGIGIVETQRAVVFSGDRPLTLASGAILPEVEVAYETYG
                     ELNPDRSNAVFICHALSGDAHAAGLHEGDDPSRPGWWDNIVGPGRPLDTDRFFVISAN
                     VLGGCKGTTGPSSIDPATGKPYGLRFPLVQVRDLVEVHRALLRELGIDHLLAAIGGSL
                     GGMQALQWAIDHPDEVDGAAIIAASARLSAQNIAFSAVAREAIMRDPDFAGGDYYERE
                     RRPDPGLALARMIGHITYLSEESMREKFGRRIQDAEVPRYGFDVDFQVESYLHYQGQS
                     FVERFDANTYLYMGRTMDYFDPFEDPVLVQNQISQGETSFLVISFDSDWRFSTEHARV
                     LASGLRGAGAPVTFQEISSPHGHDSFLFADPPEYHRTVAGYLDRLASQRGIPVPGATS
                     GSREG"
BASE COUNT          362 a          842 c          726 g          369 t
ORIGIN      
        1 agcggatggc cgcgctcgaa ggcggagtcg ccgctctcgc cctggcctcc ggccaggcag
       61 cggtcaccta ctcggtcctg aacgtctgcc ggaccggcga caacatcgtc gccctttcga
      121 ccctctacgg cggcacctac aacctcttcg cccacacgct cccccagtac gggatcgagg
      181 tcaggttcgt cgatccggac aagcccgagg agcttgagaa gcacgttgac gacaagaccc
      241 ggctggtctt cggcgagacg atcggcaatc cgcggctgaa cgtgatggac gtcgaggcct
      301 gggccaacgc tgcccacgcc cagggcctgc cgctggtggt cgacaacacc gtcgcgacgc
      361 cggttctgac cagggtcttc gagctggggg ccgacatcgc ggtccactcc gcgaccaagt
      421 tcatcggtgg tcacggaacc acgatcggcg gcgtgatcgt ggactccggc aacttcgact
      481 gggtcgccaa cgccgatcgc tttcccggcc tgacccagcc cgacccctcc taccacggcg
      541 tggtctgggc ggacgcgctc ggcccggcgg cctacatcgg ccgggtccgg accgtgctgc
      601 tccgcaacac cggcgccgcg ctctccccgt tcaacgcctt cctcctgctc cagggcgtcg
      661 agaccctgcc gctgaggatg gagcgccaca gcgcgaacgc cctcgcggtg gccgagcacc
      721 tgagtgacag cgagaacgtg ctctgggtga actacccggg cctgcccggc ttcagccacc
      781 acgaaacggc gagcaaggtg ctgaccggtg gctacggcgc cctggtcacc ttcgggatca
      841 agggcggccg cgatgccggt cgcaagttca tcgactcgct ggagctgttc tcgcacctgg
      901 ccaacatcgg tgacgccaag tcactggcga tccacccggc gaccacgacc cactcgcagc
      961 tgaatgacga agagctcgag gattccggcg tgaccgcgga cacggtgcgg ctctcggtcg
     1021 ggatcgagac gatcggggac atcctcgccg acatcgacca ggccctggcc aaggcaagct
     1081 agttgccgga cgcttccagc ggcatcggca ttgtcgagac gcagcgggcg gtcgtctttt
     1141 ccggcgaccg cccgctgacc ctcgcctccg gggcgatcct gccggaagtc gaggtcgctt
     1201 acgagaccta cggcgagctc aaccccgacc gctcgaacgc ggtcttcatc tgccacgcac
     1261 tttccggcga cgcccacgcg gcggggctcc acgagggtga cgatccttcc cggcccggct
     1321 ggtgggacaa catcgtcggc cccggccggc cgctggacac cgaccgcttc ttcgtgatca
     1381 gcgcgaacgt gctcggcggc tgcaagggca cgaccggccc ctcgtcgatc gaccctgcca
     1441 ccggcaagcc gtacggcctg cgcttcccgc tggtccaggt gcgtgatctg gtcgaggtcc
     1501 accgggccct gctgcgggaa ctggggatcg atcatctcct cgcggcgatc ggcggctcgc
     1561 tcggtggcat gcaggccctg cagtgggcga tcgaccatcc cgatgaagtg gacggcgccg
     1621 cgatcatcgc cgcctcggcc cggctttcgg cccagaacat cgccttctcc gcggtcgcgc
     1681 gggaagcgat catgcgcgat ccggacttcg ccggcggcga ttactacgaa cgcgaacgcc
     1741 ggcccgaccc cggcctggcc ctggcccgga tgatcggcca catcacctac ctctcggagg
     1801 agtcgatgcg ggagaagttc ggccggcgga tccaggatgc cgaggtgccg cgttacggct
     1861 tcgacgtcga cttccaggtc gagagctacc ttcactacca gggccagtcc ttcgtcgagc
     1921 gcttcgacgc gaacacttac ctgtacatgg gccggaccat ggactacttc gaccccttcg
     1981 aagacccggt cctggtccag aaccagatca gccagggcga gacttccttc ctcgtgatct
     2041 ccttcgattc cgactggcgc ttctcgaccg agcacgcccg ggtgctggct tccgggctgc
     2101 gcggcgccgg agctccggtc accttccaag agatctcctc cccgcacggc cacgactcgt
     2161 tcctcttcgc cgacccgccc gagtaccacc ggaccgttgc cgggtacctg gaccggctcg
     2221 cctcgcagcg gggaatcccg gtccccggcg ccacttccgg gagccgggaa ggttgagacc
     2281 ggacctcgac atcgtcgcc
//
LOCUS       DAOQMF010000281         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30033_length_2297_cov_3.303747, whole genome shotgun sequence.
ACCESSION   DAOQMF010000281 DAOQMF010000000
VERSION     DAOQMF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30033_length_2297_cov_3.303747"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1139)
                     /locus_tag="PLV77_05510"
     CDS             complement(114..1139)
                     /locus_tag="PLV77_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNC15337.1"
                     /translation="MTEHRDDSEMSRFDREGVRHFTARQSVLAVAIVAAILLVLSGGS
                     ILSAADEEKPGVEKDLLHAVGKPGSTLADNPLGRASAKLTSNLSPDEKLDPSQSFAQT
                     GTGAVTAGQIPPVTPSYFDPVELDDKAPPRQPLESLLVTGDSLSQPLDAELARRLTPE
                     GIEVTRDAHVGTGISNASLADWGQLSAAQVAEHHPDAVVVFIGANEGYPMPGPDGKDV
                     ACCSPEWAAIYANRARQVITNYRQGEKAKVYWLTVPLPRDPARQRIASVVNQAVAVAA
                     QPWRSQVRIVDTDEYFAPEGTYSDSIPVDGKDQIVRESDGIHLNDLGSSVLADHLLDR
                     IDLDFTR"
     gene            complement(1151..>2297)
                     /locus_tag="PLV77_05515"
     CDS             complement(1151..>2297)
                     /locus_tag="PLV77_05515"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNC15338.1"
                     /translation="LDLALLGVFKYYAFFVQDWADLLDSFHLGAPLPLLTIALPIGVS
                     FFTFQAITYVVDVKRGDAEPASTTDVAIYLSFFPHLVAGPIVRASEFLPQLKAPRDPN
                     KVAVGAGLALVGLGVIKKVLIADYLARVLVDPVFAVPQAYAAPDALLAAYGYAVQIYC
                     DFSGYTDIAIGLALLMGFIFPQNFNRPYRATGFRDFWRRWHMTLSRFLRDYLYIPLGG
                     SRGSRLFTYRNLMITMILGGLWHGAAWTFVLWGAFQGAALVTEHALDGRVGRRTPVWL
                     RWFLTFNLVTFAWILFRSQNLGLFWDYLGRFFVSGPATLYTLPVLLAIIVVIGMQLLP
                     ERPMERLQVRLEQLNPALLAVMLAATILVVGATVPSQGVPPFIYFQF"
BASE COUNT          419 a          707 c          835 g          336 t
ORIGIN      
        1 cgcggccgtc gtggccgacc agcacacggg tgaatgtggc catggctgaa tgattccgcc
       61 cccggcccga acttgcaagg ggggaatcta cggaaccggc aagtgaccgc ggctcagcgg
      121 gtgaagtcga ggtcgatccg gtcgagcagg tggtcggcga ggacggatga tccgaggtcg
      181 ttgaggtgga tcccgtccga ctcccggacg atctggtcct tgccgtcaac cgggatcgaa
      241 tcgctgtagg tcccctccgg agcgaaatac tcgtcggtgt ccacgatgcg gacctgcgaa
      301 cgccacggct gggcggccac ggctaccgcc tggttgacca ccgaggcgat ccgctggcgg
      361 gccggatccc gcggcaacgg cacggtcagc cagtagacct tcgccttctc gccctgccgg
      421 tagttggtga tcacctggcg ggcgcggttc gcgtagatcg cggcccactc cggggagcag
      481 caggcaacgt ccttgccgtc cggtccgggc atcggatagc cctcgtttgc gccgatgaac
      541 acgaccacgg catccgggtg gtgctcggcg acctgggccg ccgaaagctg accccagtcg
      601 gccagcgatg cattggaaat cccggtcccg acgtgagcgt cccgggtcac ctcgattcct
      661 tccggggtca gccgcctggc cagttcggcg tcgagcggct gcgagagaga gtcaccggta
      721 accagcaggc tctccagcgg ctgccgcggc ggcgccttgt catcgagctc gaccggatca
      781 aagtaggaag gagtgaccgg agggatctgc ccggcggtca ccgccccggt tccggtctgg
      841 gcgaacgact gggaagggtc gagcttctcg tccggagaga ggttcgaggt cagcttggcg
      901 ctggcccggc cgagcgggtt gtcggccagg gtcgacccgg gcttgccgac cgcatggagc
      961 aggtccttct cgacacccgg cttttcctcg tcggcggcgc tgaggatcga gccgccggaa
     1021 agcaccagca aaatcgcggc gacgatcgcc accgccagca ccgactggcg ggccgtgaag
     1081 tgccgtacgc cctcgcggtc gaagcggctc atttcactgt cgtctcgatg ttcagtcacg
     1141 gcgatgaccc ctagaactgg aagtagatga acggcggcac gccctggctg ggaaccgtgg
     1201 cgccgacgac aaggatggta gccgcgagca tgacggccag cagggcggga ttgagctgct
     1261 cgagccggac ctggagccgt tccatcggcc gttccggcag aagctgcatg ccaatcacca
     1321 cgatgatcgc gaggagcacc ggcagggtgt agagcgtcgc cggcccggag acgaagaatc
     1381 gcccgaggta gtcccagaag agcccgaggt tctgggagcg gaagaggatc caggcgaagg
     1441 tgaccaggtt gaaggtgaga aaccagcgca gccagaccgg ggtgcgtcgc ccgacccggc
     1501 cgtcaagtgc gtgctcggtg accagggcgg cgccctggaa ggcgccccag agcacgaagg
     1561 tccaggccgc gccgtgccag aggccgccga ggatcatcgt gatcatcagg ttgcggtagg
     1621 tgaacagccg gctgccgcgg ctgcccccga gcgggatgta caggtagtcg cgcaggaagc
     1681 gggagagggt catgtgccag cgacgccaga agtcccggaa gccggtcgcc cggtagggcc
     1741 gattgaagtt ctgcgggaag atgaagccca tcagcagggc cagcccgatc gcgatgtcgg
     1801 tgtagccgga gaagtcgcag tagatctgca ccgcgtagcc gtaagccgcg agcagggcgt
     1861 ccggtgcggc gtaggcctgg ggaaccgcga agaccgggtc gaccaggacc cgggccaggt
     1921 agtcggcgat caggaccttc ttgattacgc cgaggccgac cagggcgagg ccggccccga
     1981 ccgcgacctt gttcggatcc cgcggtgcct ttagctgggg aaggaactcg ctggcccgca
     2041 cgatcggccc ggcaacgaga tgcggaaaga agctgaggta gatcgcgacg tcggtggtcg
     2101 aggcgggctc cgcgtcaccg cgcttgacgt ccaccacgta ggtgatcgcc tggaaggtga
     2161 agaagctgac accgatcggc agcgcgatgg tgagcagcgg cagcggtgcc ccgaggtgga
     2221 aggagtcgag taggtcggcc cagtcctgga cgaagaaggc gtagtacttg aacaccccga
     2281 gcagggcgag gtcgagc
//
LOCUS       DAOQMF010000282         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30060_length_2296_cov_3.393574, whole genome shotgun sequence.
ACCESSION   DAOQMF010000282 DAOQMF010000000
VERSION     DAOQMF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30060_length_2296_cov_3.393574"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLV77_05520"
     CDS             complement(<1..273)
                     /locus_tag="PLV77_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNC15339.1"
                     /translation="MTASLLQMMLLGFAASLTLIVAIGAQNAYVLKLGVEGRGRILVT
                     VILICAFSDAVLIAAGVAGIGAVLEAAPAAIDAVRILGAVFLISYGV"
     gene            346..1245
                     /locus_tag="PLV77_05525"
     CDS             346..1245
                     /locus_tag="PLV77_05525"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HNC15340.1"
                     /translation="MTGFRTEHLETLVALVEEGTFEAAARRLSVTPSAISQRIKAMER
                     STGQVLVQRSNPVEPTTAGDIALRYGRQMRLLEDEAARALALEESGGGAVTVPLAINA
                     DSLGTWFMEALARVDSPGVVFEVFREDQEHTTSLLRAGKVMAAVTSNAEAVQGCRSEL
                     LGTMTYHPVCSPGFLEGQLGGEATPANLGKATVVDFDRKDQTQNRFFREFTGKPLRAP
                     RHYIPTTADYARAILLGLGWGLLPDRQCLEEIKRGDLVEFAPDQAIEMPLYWQRWTLE
                     SPLLEVLTEAVKGVAAREMHPRD"
     gene            1279..>2296
                     /locus_tag="PLV77_05530"
     CDS             1279..>2296
                     /locus_tag="PLV77_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC15341.1"
                     /translation="MATLRSRIALALIKKQLPKIVAADPVAELGDGLHVAVVGSGSPL
                     PDDKRGNPCTLVIAGEKVFVVDAGEGASETINRMGIDPGRIEALLLTHFHSDHIGGIP
                     SVNLQRWVADGTDTPMPVLGPPGAERVVKGFNDAYRLDRSYRTAHHGEDYVDPAIGPM
                     AVEEFPVPGEGESIVVLEDDGLVVSAFEVDHSPVAPVVGFRFDYQGRSAVISADTVYS
                     GNLVKAAKNTDLLIHDALSIELLMMVAEAQTEAGNPARGKILADVHDYHATAPQAADA
                     ARQAGARALALTHVVPPLPLKGLEDIYLADAPDRFDGPLWIAADGDLYSLPAAGDLKR
                     SNLIS"
BASE COUNT          407 a          787 c          754 g          348 t
ORIGIN      
        1 cactccgtag gagatcagga agaccgcgcc gaggatgcgg accgcgtcga tcgccgcggg
       61 agccgcctcg agcacagctc cgattccggc gacgccggcg gcgatcagaa ctgcgtccga
      121 gaaggcgcag atcaggatca ctgtgaccag gatgcgcccg cgaccttcca cgccgagctt
      181 gagcacgtag gcgttctggg ccccgatcgc gacgatcagg gtgaggctgg cggcgaagcc
      241 cagcaacatc atctggagga gggaggcagt catgcggagt ccgattctgg ggccgtccgc
      301 gtgtgaagtc aaactaaact ttcttatcta tctttagcgc tgctaatgac cggcttccgc
      361 accgaacatc tcgaaaccct ggttgccctg gtcgaggaag ggacctttga ggccgcggcc
      421 cggcgcctct cggtcacccc gtcggcgatc tcgcagcgga tcaaggcgat ggaacggtca
      481 accggtcagg tgctggtcca gcggagcaat ccggtcgaac cgacgacggc cggcgacatc
      541 gccctccgct acggccgcca gatgcgactg ctggaagacg aggccgcccg cgccctggcc
      601 ctggaagaaa gcgggggcgg cgcggtgacg gtgccgttgg cgatcaacgc ggacagcctc
      661 gggacctggt tcatggaggc cctggcgcgg gtcgactcgc cgggggtggt cttcgaggtc
      721 ttccgcgagg accaggaaca cacgacctcc ctgctcaggg ccggcaaggt gatggcggcc
      781 gtgacctcga atgcggaagc ggtccagggc tgccgctccg agctgctcgg aaccatgacc
      841 taccacccgg tctgttcacc cggcttcctc gaagggcagc ttggcggcga agcgacgccg
      901 gccaacctcg gcaaggccac ggtggtcgac ttcgaccgca aggaccagac ccagaaccgt
      961 ttcttccggg aattcaccgg caagccgctc cgggcgccgc ggcattacat cccgaccacc
     1021 gccgattacg cccgggcgat cctgctcggc ctgggctggg gccttctgcc ggaccgccag
     1081 tgcctggaag agatcaagcg gggagacctg gtcgagttcg ccccggacca ggcgatcgag
     1141 atgccgctct actggcaacg ctggaccctg gagtcgccgc tcctggaggt gctcaccgag
     1201 gcggtcaaag gggtcgcagc aagggagatg cacccccggg actagaaatt gacgcgcgtc
     1261 aactatagga ttcggatcat ggccacccta cgctcccgaa tcgccctagc cctgatcaag
     1321 aagcagcttc cgaagatcgt cgcggccgac ccggtcgccg aactcggcga cggactccac
     1381 gtcgcggtcg tcggctcggg ctctcccttg cccgacgaca agcgcggcaa tccctgcacc
     1441 ctggtcatcg cgggtgagaa ggtctttgtg gtcgacgcgg gcgaaggggc ctcggaaacg
     1501 atcaaccgga tgggcatcga cccgggccgg atcgaggccc tgctgctgac ccacttccac
     1561 tccgatcaca tcggcggcat cccctcggtc aatctccagc gctgggtggc cgacggcacc
     1621 gacacgccga tgccggtcct gggaccgccc ggggccgaaa gggtcgtgaa ggggttcaac
     1681 gacgcctacc ggctcgaccg cagctaccga accgcccatc acggcgagga ctatgttgac
     1741 ccggcgatcg ggccgatggc ggtcgaggag ttcccggtcc ccggcgaagg ggaatcgatc
     1801 gtggtcctcg aagatgacgg gctggtcgtg tccgccttcg aggtggacca ttcgccggtc
     1861 gcgccggtgg tcggtttccg cttcgactat cagggccgga gcgccgtgat cagtgccgac
     1921 accgtctatt cggggaacct cgtcaaggcc gcgaagaaca cggacctgct gatccacgat
     1981 gcgctttcga tcgaacttct gatgatggtc gcggaggcac agaccgaagc cggcaatccg
     2041 gcccgtggca agatcctcgc cgacgtgcac gactaccacg cgaccgcccc ccaggccgcc
     2101 gatgccgccc ggcaggccgg cgccagggcg cttgccctca cccacgtcgt gcccccgctg
     2161 cccctcaagg gcctcgaaga catctacctg gccgacgccc cggaccggtt cgacggaccc
     2221 ctctggatcg ccgccgacgg tgacctctac agcctccctg ccgcggggga cctgaagcgc
     2281 agcaacctga tcagcc
//
LOCUS       DAOQMF010000283         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30127_length_2293_cov_2.873548, whole genome shotgun sequence.
ACCESSION   DAOQMF010000283 DAOQMF010000000
VERSION     DAOQMF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30127_length_2293_cov_2.873548"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PLV77_05535"
     CDS             complement(<1..1090)
                     /locus_tag="PLV77_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15342.1"
                     /translation="MPFLKIPGRATGPANSEVRRVLSRTRAVTFGLLLGLVFSLVRVG
                     AAGADDLQVADAATGHRGQVTIIVLPPGTSPADLSGLEHAAIGLMNPGLGDVPAQQTW
                     IDVSQGGRAFDSSYDTPLDRVYPGGEFAHGWEKVLKRARSTDSKVIPGLMATVLSQND
                     LDAVAADSAGAAALAVARRDGGVARAPQACGGPASMLNADSPGAKSAIRGCPDPVTVL
                     SMPLDSAFEAAANRNPRDMQIFIERPPADSGDQLSIAIAGPGMDGELRSPSTRTDGYV
                     LSTDIAPTILRHFDIKAPKAMTGLPIESGGELDIDRLADLESRYEEVGQRRGAALAIP
                     LLLWIGLAALVSFGSRGLYSRPALQLLCL"
     gene            1089..2012
                     /locus_tag="PLV77_05540"
     CDS             1089..2012
                     /locus_tag="PLV77_05540"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC15343.1"
                     /translation="MATETIGPEAPVLPDVQYAQVGEVEIAFDVHGDPNDPVLLVIPG
                     LGTQMVYWDMPLVEMLTDRGFRVVRIDNRDNGASTVLHHKGKPALPAMMFGIPRDLAY
                     TLNDMANDAAGVLDHLGVDAAHVIGYSMGGMISQTMAIDHPDRVLSLTSMMSRTGARL
                     DAFPGVKELAALMRTRPTDSLDSYLTGMEALAAIIGSPDFPTNKERLRDIATVAYHRG
                     IHLEGTARQLHAINCQPKRDRALSRLDIPTVVLHGSADKLVFPRGGRATARAIPGAEL
                     RIYEGMGHDIPEQLWPRFVEEIDLVARRAGK"
     gene            2061..>2293
                     /locus_tag="PLV77_05545"
     CDS             2061..>2293
                     /locus_tag="PLV77_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNC15344.1"
                     /translation="MSNPGTPENPLRVAIIGAGPSGFYAADQIIKDPETHAQVDLFDR
                     LPTPYGLVRGGVAPDHPKIKSVTRVYEKTAAKEG"
BASE COUNT          399 a          781 c          745 g          368 t
ORIGIN      
        1 cgaggcagag gagctggagc gccggtctcg agtagaggcc gcgactgccg aacgagacca
       61 gggccgccag cccgatccag agcagcagcg gaatcgcgag cgccgcgccg cgccgctggc
      121 cgacctcctc gtagcggctt tcgagatcgg ccagccggtc gatgtccagc tcgcctcccg
      181 actcgatcgg caatccggtc atcgccttgg gggccttgat gtcgaagtgc cgcaggatcg
      241 tcggggcgat gtcggtcgag agcacatatc cgtcggtcct cgtactcggt gacctgagtt
      301 caccgtccat gcccgggccg gcgatggcga tcgaaagctg atcgccggag tcggccgggg
      361 ggcgctcgat gaatatctgc atgtcccgcg gatttcggtt tgcggcggct tcgaaggcag
      421 aatcgagcgg catcgaaagg acggtgaccg ggtccgggca gccgcggatc gccgacttcg
      481 cccccgggga atcggcgttg agcatcgaag ctgggccacc gcaggcctgc ggggcgcggg
      541 cgaccccccc gtcacggcgg gccaccgcca gcgcggctgc tccggccgag tcggcagcca
      601 cggcgtcgag gtcgttctgg ctgaggacgg tggccatcag ccccggaatt accttgctgt
      661 ccgtgcttct ggcccgtttc agaacttttt cccatccgtg cgcgaattcc ccgcctggat
      721 acacgcggtc taacggtgtg tcatacgaag agtcaaacgc cctccctccc tgtgagacgt
      781 caatccaggt ctgctgggcc gggacgtcgc ccagaccggg gttcatcagg ccgatcgccg
      841 cgtgttcgag cccggacagg tcggcgggcg aggttccggg aggcagaacg atgattgtca
      901 cctggccgcg atggccggtc gcggcgtcgg cgacctggag atcatcggcc ccggccgcgc
      961 cgacccggac gagcgaaaac acaaggccga gcagcagacc gaaggtgacc gcgcgggtgc
     1021 gcgaaaggac ccgtcggact tcggaattcg ccggtccggt ggccctcccc ggtatcttca
     1081 gaaacggcat ggcaacggaa acgataggtc ctgaagcccc cgttctaccc gacgtccagt
     1141 acgcgcaggt cggtgaggtc gaaatcgcct ttgatgttca cggcgatccg aacgaccccg
     1201 tgttgcttgt aattcccggc ctcggcaccc agatggtcta ctgggacatg cccctcgtcg
     1261 aaatgctgac cgaccgcggt ttccgcgtag tccggattga caaccgggac aacggcgctt
     1321 cgacggtgct tcaccacaag ggcaaacccg ccctgccggc gatgatgttc gggatccccc
     1381 gcgacctcgc ctacacgctc aacgacatgg cgaacgatgc ggccggcgtg ctcgaccacc
     1441 tcggggtcga tgccgcccac gtgatcggtt actcgatggg cggcatgatc tcccagacca
     1501 tggcgatcga ccatccggac cgggtccttt cactgacctc gatgatgtcc cggaccggtg
     1561 cgcggctcga cgcgttccct ggcgtgaagg aactggccgc cctgatgcgg acccgcccga
     1621 ccgacagcct ggacagttac ctgaccggca tggaagcgct ggcggcaatc atcggttcgc
     1681 ctgatttccc gaccaacaag gaacggcttc gggatatcgc cacagtcgcc tatcaccggg
     1741 ggattcacct cgagggaacg gcgcggcagc tccacgccat caactgtcag ccgaagcggg
     1801 accgggccct gagccggctc gacatcccca cggtcgtcct tcacggctcc gccgacaagc
     1861 tggtcttccc gcgcggcggc cgggccaccg cccgtgccat tcccggcgcg gagctccgga
     1921 tctacgaagg aatgggccac gacatccccg agcagctctg gccccgcttc gtcgaggaga
     1981 tcgacctggt cgcccggcga gccggcaagt agccggggtc acgatcgcgg tggcagggat
     2041 tccttatgct ggatgaccgc atgagtaacc caggaacccc cgaaaacccg cttcgggtag
     2101 cgatcatcgg cgccggacca tccggcttct acgctgccga ccagatcatc aaagatcccg
     2161 agacccacgc ccaggtggat ctctttgacc gcctgcccac cccctacggg ctggtgcgcg
     2221 gcggtgtcgc cccggaccat ccgaagatca agtcggtgac ccgggtctac gagaagaccg
     2281 ctgccaagga ggg
//
LOCUS       DAOQMF010000284         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30145_length_2292_cov_3.679481, whole genome shotgun sequence.
ACCESSION   DAOQMF010000284 DAOQMF010000000
VERSION     DAOQMF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30145_length_2292_cov_3.679481"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /gene="aroF"
                     /locus_tag="PLV77_05550"
     CDS             <1..1024
                     /gene="aroF"
                     /locus_tag="PLV77_05550"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNC15345.1"
                     /translation="MIVMKEGATEEEIAHVVERVEEIGCSAHISEGELLTVIGAIGDR
                     GKVADLGLEGAPGVDHVTPISKPYKLASRETKQGADSVLEIGGRKVGGTNFALMAGPC
                     TVENRDQMLDSADAVADAGATMLRGGAYKPRTSPYSFQGLGREGLRLLSEARERTGLP
                     IVTELLDLRDIDDVVEVADVIQIGARNMQNYALLSEAGKSGKAVLLKRGLSASIEELL
                     MASEYILKEGNENVMLCERGIRTYETAYRFTLDLLAVPALKEQTHLPVIVDPSHAPGR
                     RDWVESMSLAAAASGADGIIIEVHNEPEKAICDAAQQVPTSEFQELTNKLETITSVIG
                     KTISRA"
     gene            1035..2108
                     /locus_tag="PLV77_05555"
     CDS             1035..2108
                     /locus_tag="PLV77_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNC15346.1"
                     /translation="MKLAVLGVGLIGGSIGLAARDRDLAEVTGWVRNPDRAEECLKVG
                     AVDHMAATVEEACADADVIVCCASVGHLIEMGRKALEAAPEGCVVTDVGSTKQELVEA
                     LGNDRRFIGGHPLAGAETAGVMNARADLYEGARWYLTPTSDSEGMLLDRLQKLVKGLG
                     AKPVAIDAVEHDRAMAVVSHLPHVFANQLAAQGRPGTERLGALAPSLRDGTRVAGSNP
                     AIWGEIFATNHEAVVAAIDESIAGLERAREVIGSADPGRIEQWQAAVGEDRAALSATE
                     TAGEATHELRVLVPNQPGVLAELALALGRAGVNITDMSLDPAPDMSSGAISLWVAGEG
                     EAEKAEECIAGLGHSVSQVVTGS"
     gene            2125..>2292
                     /locus_tag="PLV77_05560"
     CDS             2125..>2292
                     /locus_tag="PLV77_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15347.1"
                     /translation="MSGHEVELDGGERATFRPAGPLRGSLTPPADKSISHRAAIIGAI
                     ADGPTRVTNFLD"
BASE COUNT          399 a          741 c          811 g          341 t
ORIGIN      
        1 gatgatcgtg atgaaagagg gggcgaccga agaagagatc gcccatgtcg ttgaacgggt
       61 cgaggagatc ggctgttcgg cgcacatttc cgagggagaa ttgctgaccg tcatcggggc
      121 gatcggggat cgcggcaagg tcgccgatct cggtctggaa ggagcacccg gggtcgacca
      181 cgtgactccg atctcgaagc cctacaagct ggcgtcccga gaaacgaagc agggtgccga
      241 ctcggttctc gagatcggcg gccgaaaagt cgggggaacg aacttcgccc tgatggcggg
      301 tccttgcacg gttgaaaacc gagaccagat gctggactcg gccgacgccg tggccgatgc
      361 cggcgcgacg atgctgcgag gcggcgccta caagccccgg acctctccgt acagcttcca
      421 gggcctcggc cgcgaagggc tgcgcctgct ttccgaagcg cgcgagcgta ccggcctgcc
      481 aatcgtgacc gagctgctcg acctgcgcga catcgacgac gtggtcgaag tggcggacgt
      541 gatccagatc ggcgcccgca acatgcagaa ctacgcgctg ctttccgaag cgggcaagtc
      601 gggcaaggcc gtgctgctga agcgcgggct ttcggcctcg atcgaggagc ttctgatggc
      661 ctccgagtac atcctcaagg aaggcaacga gaacgtgatg ctctgcgaaa ggggcatccg
      721 gacctacgag accgcctacc gcttcacgct cgacctgctg gcagtgcctg ccctgaagga
      781 gcagacgcac ctgccggtga tcgtcgatcc gagccacgca ccgggtcgtc gcgactgggt
      841 cgagtcgatg tcccttgccg ctgccgcttc cggtgcggac gggatcatca tcgaggttca
      901 caacgagccc gagaaggcga tctgcgatgc ggcccagcag gttccgacct ccgaattcca
      961 ggagctgacg aacaagctgg agacgatcac ctcggtgatc ggcaagacga tctcgcgggc
     1021 ctagaggggg ttcgatgaaa ctggccgtcc tcggagtcgg gctgatcgga ggctcgatcg
     1081 gtctcgcggc acgggaccgg gatctggccg aagtcaccgg ctgggtgcgg aacccggacc
     1141 gggcggagga atgcctcaag gtcggggcgg tcgatcacat ggcggccacg gtcgaggagg
     1201 cctgcgccga cgcggacgtg atcgtctgct gcgcctcggt cgggcatctg atcgagatgg
     1261 gccgcaaggc tctcgaggct gcccccgaag gctgcgtggt gaccgatgtc ggttcgacca
     1321 agcaggaact ggtcgaagcg ctcggaaacg accggagatt catcgggggt cacccgctgg
     1381 ccggcgccga gacggccggg gtgatgaacg cccgtgccga tctctacgag ggtgcccgct
     1441 ggtacctgac cccgacctcg gactccgagg ggatgctgct tgaccggctg cagaagctgg
     1501 tcaagggcct cggcgcgaaa ccggtcgcga tcgacgccgt cgagcacgac cgggcgatgg
     1561 cggtggtcag ccacctgcca catgtatttg ccaatcagct cgccgcccag ggtcggccgg
     1621 gcaccgagcg gctcggcgcc ctcgctccga gcctccgtga cggcacccgc gtcgccggct
     1681 cgaaccccgc gatctggggc gagatcttcg ccaccaatca cgaggcggtg gtcgccgcga
     1741 tcgacgagtc gatcgccgga ctcgagcgcg ccagggaagt gatcggctcg gccgaccccg
     1801 ggcggatcga gcagtggcag gccgccgtcg gcgaggaccg ggcggcgctt tcggcaaccg
     1861 agaccgccgg tgaagcgacc cacgagctgc gggtgctggt cccgaatcag cccggcgtgc
     1921 tggccgaact ggccctcgcc ctgggccggg ccggggtcaa catcaccgac atgtcgctcg
     1981 accctgctcc cgacatgagc tcgggtgcga tctcgctctg ggtcgccggg gagggcgagg
     2041 ccgagaaggc cgaagaatgc atcgccgggc tcggccactc ggtcagccag gtagtgaccg
     2101 gatcatgatc cggcggtggc tccggtgagc ggtcacgaag tcgaactcga cggcggcgag
     2161 cgggccacct tccggcctgc cgggccgctg cgcggctcgc tgaccccgcc ggccgacaag
     2221 tcgatctccc accgggcggc gatcatcgga gcgattgccg acggcccgac ccgggtcacc
     2281 aacttcctcg ac
//
LOCUS       DAOQMF010000285         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30369_length_2283_cov_3.821813, whole genome shotgun sequence.
ACCESSION   DAOQMF010000285 DAOQMF010000000
VERSION     DAOQMF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30369_length_2283_cov_3.821813"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLV77_05565"
     CDS             complement(<1..757)
                     /locus_tag="PLV77_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15348.1"
                     /translation="MTHRIPHPKRILLFLTVAIAALIGFAGTASAAGQGSIVFIKNND
                     IWLANPDGSHQFRVTWDGSEDFPYLSPSQAADGTIAAGHVRDIVLMRQNGDVIREIEP
                     PALINSVSHPMDGVPVDVAISPNGKRIAWSFVGYECDGTVNCGPRTATGYTDSSGRTP
                     VSKYGSTYFHNPSWVGNSRTLQGGGFGSQVMIHDLGQDPVHWFDDSDYAEYSTDLGDS
                     ELSPDGSRLASIRGYGSETQLYTFEVSGNATSGP"
     gene            complement(881..1501)
                     /locus_tag="PLV77_05570"
     CDS             complement(881..1501)
                     /locus_tag="PLV77_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNC15349.1"
                     /translation="MRRLLPLILGLALLWAPAQAGAVTAQTARFVAGFEGFVSCPYAD
                     PAGHATIGYGHLLHYGPPTRADRRKWGCLTQAQALRLLRQDLRETEIEVFDRIRGAKV
                     NAPMVTALTSFAFNLGAGYLDFVAKRGSRPATNIGRAIRVGKYRRAARQMLYFDGVIV
                     GGKRYELEGLQIRRRKEYRLMIRGIHQLRACDGACSDGPDGGLGVN"
     gene            complement(1566..2009)
                     /locus_tag="PLV77_05575"
     CDS             complement(1566..2009)
                     /locus_tag="PLV77_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC15350.1"
                     /translation="MAIKRMDNVLIVVEDMAAVKDFFIELGMEFDGETTVEGPWVDQT
                     VGLEGVRADITMLRTPDGGCRVELSRFHNPPAVRAEPEDAPSNALGIRRIMFAVDDID
                     EVVERLVSRHEAKLVGEIVRYGDSYRLCFLRGPEGIVVGIAEELG"
BASE COUNT          371 a          740 c          771 g          401 t
ORIGIN      
        1 ccggtcccga ggtcgcgttg ccgctcacct cgaaggtgta gagctgggtc tcggatccgt
       61 agccgcggat gctggccagt cgggagccgt cgggagagag ttcgctgtca ccgagatcgg
      121 tcgagtactc ggcgtagtcg ctgtcgtcaa accagtggac cgggtcctgt ccgaggtcgt
      181 ggatcatcac ctgggagccg aagcctccgc cctgaagggt gcgggaattg ccgacccagg
      241 aggggttgtg gaagtaggtc gagccgtatt tcgagaccgg ggtccggccg ctcgagtcgg
      301 tgtagccggt tgcggtgcgc ggcccgcagt tgaccgtccc gtcgcattcg tagccgacga
      361 aggaccaggc gatccgcttg ccgttcggcg agatcgccac gtcaaccgga acgccgtcca
      421 tcggatggct gaccgagttg atcagggccg ggggttcgat ctcccggatc acgtctccgt
      481 tttgccgcat cagcacgatg tcgcgcacgt gcccggccgc gatcgtcccg tcggcggcct
      541 ggctggggga gaggtagggg aagtcctcgc ttccgtccca ggtgacccgg aactgatggc
      601 tcccgtcggg attggcgagc cagatgtcgt tgttcttgat gaagacgatc gatccctgcc
      661 cggccgccga ggcggttccg gcaaagccga tcagggccgc gatcgcgacg gtcaggaaca
      721 gaagaattcg cttgggatgg gggatgcggt gggtcattgg tctcctcgtc gggtgctgtg
      781 gaaagcaacc tagaaagggg accttgcccg gatcttgcgg actgattgcg ccgggccggg
      841 aggccgctgc ccgggaccag ccggcgcctg ccggacccgg tcagttgacg cccaggccgc
      901 cgtccgggcc gtcgctgcag gcgccgtcgc aggctctcag ctggtggatg ccgcgaatca
      961 tcagccggta ctccttgcgc ctcctgatct gaaggccctc gagctcgtac cgtttgccgc
     1021 cgacgatgac gccgtcgaag tagagcatct gccgcgccgc gcgccggtac ttgccgaccc
     1081 ggatcgcgcg gccgatgttg gtcgccggac ggctgccccg cttcgccacg aagtcaaggt
     1141 agcccgctcc gaggttgaag gcgaacgagg tcagcgccgt gaccatcggc gcgttcacct
     1201 tggctccccg gatccggtca aagacctcga tttcggtttc tctcaggtcc tggcgcagca
     1261 gccggagggc ctgcgcctgg gtcaggcagc cccacttgcg gcgatcggcc cgggtcggcg
     1321 gcccgtaatg gagcaggtgc ccgtagccga tcgtggcgtg gccggccggg tcggcgtagg
     1381 ggcaggaaac gaacccttcg aagccggcca cgaacctggc ggtctgcgcc gtgacggcgc
     1441 ccgcctgggc cggggcccag agcagggcca ggcccagaat gaggggaagg aggcgacgca
     1501 tcgcctcaat ggatagcagt ccgggcgccc gcgacgcggc acaggagacg cgcggaaccc
     1561 cgctttcagc cgagttcttc ggcgatcccg accactattc cctccggtcc ccgcagaaaa
     1621 cagagccggt agctgtcccc gtagcgcacg atctcaccga ccagcttcgc ttcatgccgg
     1681 ctgaccagac gctcgaccac ctcgtcgatg tcgtcgaccg caaacatgat ccggcggatc
     1741 ccgagggcgt ttgacggcgc gtcttccggt tcggccctga ccgccggcgg attgtgaaat
     1801 ctcgaaagct ccacccggca gcctccatcc ggcgtccgca gcatggtgat gtccgcccgg
     1861 acgccttcaa gccccacggt ctggtccacc cagggcccct cgaccgtcgt ctccccgtcg
     1921 aactccatgc cgagttcgat gaagaagtcc tttaccgccg ccatgtcctc gaccacgatg
     1981 aggacgttgt ccattcgctt tattgccact ttgatcctct ccttgccgga tgccggaccc
     2041 gaaccatggc agacggaacc ggcccgcccg tggggcaggc cggaaccgga tgcatgctcc
     2101 taatcggagc ctcatataac caggacatgc atacgagact tcgtcaagct aatcggttgc
     2161 ggaagcggat tctacgggct ggagggtcgg ctcgacccca ggttcggtcg gtgaagctga
     2221 aggcgggaga tattcagcgg atggtgctgc gggtgctaga gggatcggag tctccacttc
     2281 atg
//
LOCUS       DAOQMF010000286         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30377_length_2283_cov_3.248205, whole genome shotgun sequence.
ACCESSION   DAOQMF010000286 DAOQMF010000000
VERSION     DAOQMF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30377_length_2283_cov_3.248205"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLV77_05580"
     CDS             complement(<1..220)
                     /locus_tag="PLV77_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNC15351.1"
                     /translation="MEELYREQILEHYKRPHNFGAVENPDLEFEDTNPFCGDEQRVTI
                     RLDEDDRVAEVKFEGQGCAISTAATSLLT"
     gene            complement(274..567)
                     /locus_tag="PLV77_05585"
     CDS             complement(274..567)
                     /locus_tag="PLV77_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15352.1"
                     /translation="MDPEDDLVIFPREDVEACLRNEKMQQEANSDGSPSGQSQAMETS
                     RLGAIEAGAPERIRWIYFESPEWTWQNLCGRSGWLLIDPETIDQYDFVMTMMN"
     gene            complement(611..1660)
                     /locus_tag="PLV77_05590"
     CDS             complement(611..1660)
                     /locus_tag="PLV77_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15353.1"
                     /translation="MEVKLGVPEGEADEFDTLPPADLPAHEKKLIDARNHLVQTSQAA
                     RIWAEENPAHPVSYLVTRSDVSEQTKIQALRRIESVSLPFQVNELLDAARHFNECQAV
                     SQAAFDAEQPVAEDDLPDHGDRDIFEGLRAAYLNLLRGYRNWGGFGYHGWTTKRDPDS
                     YPGPAIWTRQDCVFRFGRALEREFPDRVHLGLKISKSTTESFDPAVDRRQSVDITVTV
                     PGLFSRDLSARRFRNLRHDLFVEVEWLKKGRWLNTDQLVKRCGRIQQNLINLQRSIDS
                     GRCEFAAMLIVDDEGFLDFHGDLLRWPPDVIPLVVSPGEVVELGLDDGSVEPVLDEIE
                     SLHDSACPCSDGPLA"
     gene            complement(1760..>2283)
                     /locus_tag="PLV77_05595"
     CDS             complement(1760..>2283)
                     /locus_tag="PLV77_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15354.1"
                     /translation="GASYAALRIPRNSVGAGQLKRNSVSASKLRKGSVNSSKVKDRSI
                     RSRDLAPGVIPGATWRGGRDSSTLLDLTGTMQVVASTPVLPAGSYVVWSRANVLGGVG
                     SSTIICSVASDAAQNITIPAGAVFPLSMAATKVLEEPGPIELNCNKSSGSPQVAQAQV
                     IATRVPKLTAPPE"
BASE COUNT          389 a          712 c          755 g          427 t
ORIGIN      
        1 cggtgaggag agaggtggct gcggtggaga tcgcacagcc ctggccttcg aacttgacct
       61 cggcgacccg gtcgtcttcg tcgaggcgaa tcgtgacgcg ctgctcgtcg ccgcagaagg
      121 ggttcgtgtc ctcgaactcg aggtccgggt tctcgaccgc cccgaagttg tgggggcgct
      181 tgtaatgctc gaggatctgt tcgcggtaga gctcttccat cggtcagacg aggttagctg
      241 accgccgctc ccaggccacg aacccttgcc gggtcagttc atcattgtca tcacgaagtc
      301 gtactggtca atcgtttccg gatcgatgag caaccagccg gagcgcccgc aaaggttctg
      361 ccaggtccat tcgggtgact cgaagtagat ccaccggatt cgctcgggtg ctccggcctc
      421 aatcgcgccg agccgactcg tttccatcgc ctgactctgc ccggacggag aaccgtccga
      481 attggcttcc tgctgcatct tctcgttccg caaacacgct tcaacgtctt cccggggaaa
      541 gatcaccagg tcatcttccg ggtccatgag ttgagagtcg cgagcccggc ccggacctga
      601 cagggtccgg tcaggccagg gggccgtcgg agcaggggca ggcggagtcg tggagggatt
      661 cgatttcgtc gaggaccggt tcgaccgagc cgtcgtcgag gccgagctca accacctctc
      721 ccgggctgac caccagggga atcacgtccg gcggccacct gagaaggtcg ccgtggaagt
      781 cgaggaagcc ttcgtcgtcg acgatcagca tcgcggcgaa ctcgcagcgc ccgctgtcga
      841 tcgagcgctg caggttgatc aggttctgct ggatccggcc gcagcgcttg accagctggt
      901 cggtgttcag ccagcggccc ttcttcagcc actcaacctc gacgaagaga tcgtggcgca
      961 ggttccggaa ccggcgtgcg ctcaggtccc tggagaacaa tccgggcacg gtgaccgtga
     1021 tgtccacgga ctgccggcgg tcaaccgccg ggtcaaagct ctccgtcgtc gacttgctga
     1081 tcttcaggcc gaggtgaacc cggtccggaa actcccgctc cagtgcccgt ccgaaccgga
     1141 acacgcagtc ctggcgggtc cagatcgccg ggccgggata gctgtccggg tcccgcttcg
     1201 tggtccagcc gtggtagccg aagccgcccc agttgcggta gccgcgaagc aggttgagat
     1261 aggcagcacg caggccttcg aagatatccc ggtcaccatg gtctgggagg tcgtcctcgg
     1321 cgactggctg ctcggcatca aaggcggcct gggaaacggc ctggcattcg ttgaagtggc
     1381 gggccgcgtc aaggagttcg ttcacctgga agggcagtga aaccgactcg atcctccgca
     1441 aggcctggat cttggtttgt tcactgacgt cgctgcgggt gacgagatag gaaacagggt
     1501 gggccgggtt ctcctcggcc cagatccggg ccgcctggct ggtctggacc aggtggttcc
     1561 gggcgtcaat cagtttcttc tcgtgggccg gcaaatcggc aggtggaagc gtgtcgaact
     1621 cgtcggcttc tccctccggc acccccaact tgacttccat gcgcgaaatc gtagatgtgg
     1681 tctgctgttg cggggggtga aacgggtcag actgaaggca gtcctgcacg gggacggtga
     1741 cgttccccgg ggagcggaat cattccgggg gtgcggtgag tttcgggacc cgcgtcgcga
     1801 tgacctgggc ctgcgccacc tgcggggaac cgctggactt gttgcagttg agctcgatcg
     1861 ggccgggctc ttcgaggacc ttggtcgctg ccatcgagag cgggaagacc gcacccgccg
     1921 ggatggtgat gttttgcgcc gcgtcggaag cgaccgagca gatgatcgtc gaactgccga
     1981 cgccgccaag gacattcgcc cggctccaga cgacatatga accggccgga agcacgggcg
     2041 tgctcgcaac cacctgcatc gtcccggtca gatcaagcag cgtcgaggag tcccggcctc
     2101 ctcgccaggt ggcgccggga atcactcccg gcgccagatc ccgggaccgg atcgaccggt
     2161 ctttgacctt cgacgagttg accgacccct tcctcaactt ggacgccgaa acggagttcc
     2221 ttttcagttg accggcgccg accgagtttc gcggaatcct gagggccgcg tagctggcac
     2281 cac
//
LOCUS       DAOQMF010000287         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30443_length_2280_cov_4.287640, whole genome shotgun sequence.
ACCESSION   DAOQMF010000287 DAOQMF010000000
VERSION     DAOQMF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30443_length_2280_cov_4.287640"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLV77_05600"
     CDS             <1..619
                     /locus_tag="PLV77_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNC15355.1"
                     /translation="FAVMVRDLAMADDLAVLDEAARAALTATDHPIVLVPARADAYDP
                     RLSGPSRRVGLMLPYTALHHRLLSLCARPLVMTSGNRSEEPIGIDDVVECLARALKLD
                     GNSEIQIGGPDVTTYGGLMDETAKAMGRRPPLRIGVPFLTPRLSSLWLGLITPVDVGV
                     ARPLIEGLSTETVVTQPPGPEELGVQPESLGTVLRKAVAEIEAGG"
     gene            complement(616..1221)
                     /locus_tag="PLV77_05605"
     CDS             complement(616..1221)
                     /locus_tag="PLV77_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15356.1"
                     /translation="MSVRIELMPDPVRHGLARTGGVTCEQEAVITVDRETFEQIWTPS
                     TLELLARSYWDYIRRFTLGAIRVQYRVDSQTVTLLGRVPLLRFRTPVFSTGNDRASIE
                     WPVEKGLLVARDGRGKGFLRIEASRGQAEDAGCPTLMVSSTVSNFYPWLRGTGRFARF
                     GTWLYSQTQLRIHILVTKGFLRSLDELPDEVLRRGVSPGTG"
     gene            complement(1218..>2280)
                     /locus_tag="PLV77_05610"
     CDS             complement(1218..>2280)
                     /locus_tag="PLV77_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNC15357.1"
                     /translation="EHPNDAPLPLSEQWKHLHWLQAFWTRAWRENVTGLSGMVAYNLM
                     LAIFPFAFLVLFIFSQVLRVEGIDQGIINDLQRLFPNAEQGTLDDILKAIRNNSATLG
                     VAAVFGSLWIGASFWGAMDTAFCRIYHVQCRGWWEQKRFSLVMLLVVTIFLLGSVLLP
                     ALEGALIRQTDNLPFGLNEVQNLDNAVLLAVTLSLNFTVAAVIYWAVPKGHMPWRAVW
                     PGALFTTLVAAAANWLFPVYLNNVSTLNRFGSTIGFILIALFWFYLVALTMLAGAVIN
                     SIRHEYHDTGSLPYGILSRAQLLARAQADEAKAAQRPMAEPYRSLVSGDAWPKPEAGP
                     DEESEAETKVIPLRRRKRQ"
BASE COUNT          399 a          733 c          796 g          352 t
ORIGIN      
        1 cttcgccgtg atggtgcgcg acctcgccat ggcagacgac ctcgccgtgc tcgacgaagc
       61 cgcacgggcg gcgctcacgg ccacggacca tcccatcgtg ctcgtgccgg cccgtgccga
      121 cgcctacgac ccgcggctct cgggcccttc gcggcgggtg ggcctgatgc tcccgtacac
      181 ggcgctgcac caccgcctgc tctccctctg cgcgcgtccg ctcgtgatga cctcgggcaa
      241 ccgcagcgaa gagccgatcg gcatcgacga cgtggtcgag tgcctggcgc gggcgctgaa
      301 gctggacggc aacagcgaga tccagattgg cgggccggac gtgacgacct acggcggact
      361 gatggacgag accgcgaaag cgatggggag aaggccaccg ctccggatcg gtgtgccctt
      421 tctcaccccg aggctgtctt cgctctggct cggactgatc acgccggttg acgtcggggt
      481 cgcccggccg ctgatcgagg ggctttcgac cgaaacggtg gtgacgcagc cgccgggccc
      541 ggaagagctg ggggttcagc ccgaatcgct cggaacggtc cttcggaagg ccgtcgcgga
      601 aatcgaagcc ggcggctaac cggttcccgg cgatacgccc cgccggagca cctcgtccgg
      661 cagctcgtcg agcgagcgga ggaagccctt ggtcacgagg atgtggatgc gcagctgggt
      721 ctgcgagtaa agccaggttc cgaacctggc gaaccggccg gtccccctca accagggata
      781 gaagttcgaa accgtcgaag agaccatcag ggtcgggcac ccggcgtcct cggcctggcc
      841 gcgggaagcc tcaatccgca gaaaaccctt gccgcggccg tcgcgggcga ccagcagtcc
      901 cttttcgacc ggccactcga tcgaggcccg gtcgttgccg gtcgaaaaga ccggggtgcg
      961 gaagcggagc agcggcaccc ggcccagcag cgtcacggtc tgggagtcca ctcggtactg
     1021 gacccgaatc gcccccagcg tgaaacggcg aatgtaatcc cagtaggacc tggccagcaa
     1081 ttccagggtg gaaggcgtcc agatctgctc gaaggtttcg cggtcgaccg tgatcaccgc
     1141 ctcctgctcg caggtcacgc cgccggtccg ggccagtccg tgccggaccg ggtccggcat
     1201 cagctcgatc ctgacgctca ttggcgcttg cgacggcgca gcggaatgac cttggtctcg
     1261 gcctcgctct cttcgtccgg gccggcctcc ggcttcggcc aggcgtcacc gctgaccagc
     1321 gagcggtagg gctcggccat cggtcgctgt gcggccttcg cctcgtcggc ctgggccctg
     1381 gccagcagct gggcccggct gaggatgccg tagggcaggc tcccggtgtc gtggtactcg
     1441 tggcggatcg agttgatcac cgcgccggcc agcatggtca gggcgaccag gtaaaaccag
     1501 aaaagggcaa tcaggatgaa gccgatcgtc gagccgaaac ggttcagggt cgagacgttg
     1561 ttgaggtaga ccgggaagag ccagttcgca gcggcggcga ccagggtcgt gaagagggct
     1621 cccggccaga ccgcccgcca gggcatgtgg cccttcggca ccgcccagta gatcactgcc
     1681 gcgaccgtga agttcaggct gagtgtgacc gcgagcaata ccgcgttgtc caggttctgg
     1741 acctcattca ggccgaaggg aaggttgtcg gtctgacgga tcagggcacc ctcgagggcg
     1801 gggaggagga cgctgccgag caggaagatc gtgaccacca gcaacatgac gagcgagaat
     1861 cgcttctgct cccaccagcc gcggcactgc acgtgataga tccggcagaa ggcggtgtcc
     1921 atcgcccccc agaacgaggc gccgatccag agcgagccga agacggcggc cacaccgagg
     1981 gtggcggagt tgttgcggat cgccttgagg atgtcgtcga gcgtgccctg ctcggcgttc
     2041 gggaagagcc gctgaaggtc gttgatgatg ccctggtcga ttccttcgac ccgcagtacc
     2101 tgggagaaga tgaacagcac gaggaacgcg aacgggaaga tcgccagcat caggttgtag
     2161 gcgaccatgc cggagagccc ggttacgttt tcccgccagg cccgggtcca gaaggcctgc
     2221 agccagtgga ggtgcttcca ttgctcggag aggggaagcg gggcatcgtt gggatgctcc
//
LOCUS       DAOQMF010000288         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30558_length_2276_cov_3.223773, whole genome shotgun sequence.
ACCESSION   DAOQMF010000288 DAOQMF010000000
VERSION     DAOQMF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30558_length_2276_cov_3.223773"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..966
                     /locus_tag="PLV77_05615"
     CDS             199..966
                     /locus_tag="PLV77_05615"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HNC15358.1"
                     /translation="MSVFRNLEARIEGLVEGVFSRAFSSEVQPVELARKLAKEMDSHK
                     TASVSRVYVPNEYTVHLSEKDRDKLEGYERSLEQELSGYLLEHARRKGYDLLTRPEVH
                     FNTDSRLRLGEFGIQARLVKPPVREGEMPRQADEGHTMVYKVAPQPAADPTARAPERP
                     SLTRAVVDLDDRRYVLDGPRAVLGRSDDAECVLRDPNVSRRHAELRQDAAGQWEIFDL
                     GSTNGVKINGRRVTEAPLREGDQVTIGTTTFRFGIER"
     gene            985..1449
                     /locus_tag="PLV77_05620"
     CDS             985..1449
                     /locus_tag="PLV77_05620"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC15359.1"
                     /translation="MDYEPISVALKFGFLAVLFLFLLWIATSARKDLKRNSGGNFESD
                     TTIGAMPGGSYDAWLVVVRGGGLEAGTRFDVFGGVTLGRSSDADIAFTDRYASGLHAR
                     LYPRGDRYFLEDMNSTNGTLLDGGAVTSDAEVRDGSMIEIGDTAFRFELDRS"
     gene            1469..>2276
                     /locus_tag="PLV77_05625"
     CDS             1469..>2276
                     /locus_tag="PLV77_05625"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNC15360.1"
                     /translation="MLRVSGHSERTDVGRQRQANEDSYMARAPLFVVADGMGGAQAGE
                     VASLTAIQAFQAGLPEGSPEAAIEQTIGVANRNIYEQAHADPSLSGMGTTITVAAVDS
                     DSEQVIIGHVGDSRAYRLRDGIIQRLTKDHSLVEEMRRRGQITEEQAEDHPQRSIITR
                     ALGPEPEVQADLTAVPSEPGDIFLLCSDGLTTMIGDEKIRDILMGATSLDAAARTLVD
                     EANRAGGRDNITVIVFQVEDPARPLPRGEQPTMIRRGGAARGASRRAAGRR"
BASE COUNT          396 a          788 c          738 g          354 t
ORIGIN      
        1 gtccctgtcg tgagctcgcc ttccttcctc ctttcggtgg ccgtctgggc ggcggtggcg
       61 ctgcttgtcg gggccatcgt gggctggacc agagggcgaa gcggcggccc ggtccacgag
      121 ccactgacca tcgctcccgt aggatcaagt cgtgtcccga atgccaactg agccgattgg
      181 cttcacggat tcgcggcgat gagcgttttc cgcaacctcg aagcccgcat cgagggcctc
      241 gtcgaagggg tgttcagccg tgccttttct tcagaggtcc agccggttga acttgcccgc
      301 aagctggcca aggagatgga ctcccacaag accgcgtcgg tctcccgggt ctatgtgccg
      361 aacgagtaca cggtgcacct ctcggaaaag gaccgcgaca agctcgaggg ttacgagcga
      421 tctctggaac aggaactctc cgggtacctg ctggagcatg cgcgccgcaa gggttacgac
      481 ctgctgacca ggcccgaggt tcatttcaac accgacagcc ggctgcgact cggcgagttc
      541 ggaatccagg cccgcctggt caagcctccg gtccgcgagg gcgagatgcc ccgccaggcc
      601 gacgagggcc acaccatggt ctacaaggtg gcgccgcagc cggcggccga cccgaccgca
      661 cgcgcccccg aaaggccctc gctgacccgc gccgtggtcg acctcgatga ccggcgctac
      721 gtcctcgacg gaccgcgcgc cgtcctcggc cgctccgacg acgccgagtg cgtactccgg
      781 gacccgaacg tttcccgccg ccacgccgag ctccggcagg atgcggccgg tcaatgggag
      841 atcttcgacc tcggctcgac caacggggtc aagatcaacg ggcgaagggt gacggaagca
      901 ccgcttcgcg aaggcgacca ggtcacgatc ggaaccacga ccttccgctt cggaatcgaa
      961 cggtagaaag cgccggccgc gcaattggac tacgaaccga tttcagttgc cctcaagttc
     1021 ggatttctgg ccgtcctctt cctgttcctg ctctggatcg cgaccagcgc gaggaaagac
     1081 ctgaagcgga actcgggcgg caatttcgag tccgacacca cgatcggagc gatgcccggc
     1141 ggctcctacg acgcctggct ggtcgtggtc cgcggaggcg gactcgaagc ggggacccgc
     1201 tttgacgtct tcggcggggt gaccctgggc cgctcttccg acgcggacat cgccttcacc
     1261 gatcgttacg cgtccggcct tcacgcgagg ctctaccctc gcggtgaccg atacttcctt
     1321 gaagacatga actcgaccaa cggcactctg ctggacggcg gcgcggtgac atccgacgcc
     1381 gaggtccggg acgggagcat gatcgagatc ggcgacaccg ctttcaggtt tgaacttgac
     1441 cgctcatgag tagctcggaa acatccaaat gctgagagtc tcgggacatt ccgagcggac
     1501 cgacgtcggc cgccagcgcc aggccaatga ggattcctac atggcccgcg ccccgctttt
     1561 cgtggtcgcc gacggcatgg gcggtgccca ggccggcgag gttgcctcgc tgaccgcgat
     1621 ccaggccttc caggccggcc tgccggaggg cagtcccgaa gcggcgatcg aacagacgat
     1681 cggagtcgcg aaccggaaca tctacgagca ggctcacgcc gacccgtcgc tttcggggat
     1741 gggcacgacg atcacggtgg ccgcggtcga ctccgattcc gagcaggtaa tcatcggcca
     1801 cgtcggcgac tcccgcgcct accgcctccg cgacggaatc atccagcgcc tgaccaagga
     1861 ccactccctg gtcgaggaga tgcgacgccg cggccagatc accgaggagc aggccgagga
     1921 ccatccccag cgttcgatca tcacccgggc gcttggcccg gagccggagg tccaggccga
     1981 cctcaccgcg gtgccctccg agccgggcga catcttcctg ctctgctccg acggactgac
     2041 cacgatgatc ggggacgaga agatccgcga catcctgatg ggggcgacca gcctcgacgc
     2101 ggccgcacgc accctggtcg acgaggccaa ccgggccggt ggccgggaca acatcaccgt
     2161 gatcgtcttc caggtcgagg atccggcgcg gccgctgccg aggggcgagc agccgaccat
     2221 gatccgccgt ggcggcgcag cccgcggagc ttcccgccgc gccgccggtc gccgcc
//
LOCUS       DAOQMF010000289         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30640_length_2273_cov_2.466186, whole genome shotgun sequence.
ACCESSION   DAOQMF010000289 DAOQMF010000000
VERSION     DAOQMF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30640_length_2273_cov_2.466186"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1320)
                     /locus_tag="PLV77_05630"
     CDS             complement(49..1320)
                     /locus_tag="PLV77_05630"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC15361.1"
                     /translation="MTEISLDSLSGPVASYEARPEEVNRVVLLYSGGLDTSVMLHWIQ
                     QAYDAEVVTLTVNLGQPGEDYGAILDKAKAMGAVETVVIDAREEFAEDFVLPAIKANA
                     LYGGGYPLFTSLGRPLIGKLGVEVAAEYACDTLAHGCTGKGNDQVRLEGTVATLNPDL
                     KMIAPVRAWQIGREEELEYCRQNGIPLKGGTSESPYSIDDNLWGRSSEGKVIEQLDTA
                     PPDDVFQLVTRPEEAPDEPELVTIGFEAGRPVSFNGERLDLVPLLEAAAEAGCRHGCG
                     IVDHIEDRIVGLKVRDIYEVPAAALVLTAHQDLEKVVSTIHQNNFKHSLDRHWAYMVY
                     AGLWYEPLRHDLDSYMDSANEFITGEVTLKLYKGSVTPVARKSDYAVYDENLASFGAS
                     GGEFSQNAAPGFIELFSLQSRMAHQVRNRKE"
     gene            complement(1317..1772)
                     /locus_tag="PLV77_05635"
     CDS             complement(1317..1772)
                     /locus_tag="PLV77_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15362.1"
                     /translation="MRNDEPPRRRHDEDPHYTPEEQKKHLRNLIIVLIAVIVGLVIAV
                     IVISSGGDSSDDTSATTGDTAVTTPVVPVTPSGETGTTDSSGEDDGGVTPTDTGTDTS
                     GGISPDTSTGTGTDTGGVTPDTSTGTDTGGVTPDTSTDSGSSSGGIGPG"
     gene            complement(1813..>2273)
                     /locus_tag="PLV77_05640"
     CDS             complement(1813..>2273)
                     /locus_tag="PLV77_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC15363.1"
                     /translation="AVLAHDNRTDPIGACIGNPETGEVLAAGDHGSTFAAGPVASSAA
                     LAVLDLVDDPEMLRRIRALGASLTERLNALDGVAEVRGRGLMIGVRLEPGLNAGEVNA
                     ALLGNGLVANAPRPDTLRFLPPFVLTDEQADRAVALIDETLAGYHPDESN"
BASE COUNT          352 a          725 c          772 g          424 t
ORIGIN      
        1 agaaccgtct gccggaacac gggcctccgg cttgcccggc gggaacctct actccttccg
       61 gttccggacc tggtgagcca tgcggctttg aagcgaaaag agttcgatga agcccggggc
      121 ggcgttctgc gagaactctc cgccggatgc cccgaaggaa gcgaggttct cgtcgtagac
      181 cgcgtaatcg gacttgcggg cgaccggagt caccgacccc ttgtagagct tgagggtgac
      241 ttcgccggtg atgaattcgt tggctgaatc catgtaggaa tcgagatcgt ggcgcagcgg
      301 ttcgtaccag agcccggcgt agaccatgta ggcccagtgc cggtcgagcg aatgcttgaa
      361 gttgttctgg tgaatggtcg aaacgacctt ctcgagatcc tggtgggcgg tcagaacgag
      421 cgccgccgcc gggacctcgt agatgtcgcg caccttgagg ccgacgatgc ggtcctcgat
      481 gtgatcgacg attccacaac cgtgacggca accggcctcg gctgccgcct cgagcagcgg
      541 cacgaggtcg agccgctcgc cgttgaagct gaccggacga cccgcttcga atccgattgt
      601 caccagttcc ggctcgtcgg gagcctcttc cggccgggtg accagctgga agacgtcatc
      661 cggcggagcg gtgtcgagct gctcgatgac cttgccctcc gaggagcggc cccagaggtt
      721 gtcgtcgatc gagtagggcg attcggaggt gccgcccttc agcgggatgc cgttctggcg
      781 gcagtactcg agttcttcct cgcgtccgat ctgccaggcc cggaccgggg cgatcatctt
      841 gaggtcgggg ttcagggtcg ccaccgttcc ttcgagccgg acctggtcgt tgcccttgcc
      901 ggtgcagccg tgagcgaggg tgtcgcaggc gtactctgcc gcgacctcga ctccgagctt
      961 gccgatcagc ggccggccga gcgaggtgaa gagcgggtag ccgccgccgt aaagggcatt
     1021 ggccttgatc gccggcagca cgaagtcctc ggcgaattcc tcgcgggcgt cgatcacgac
     1081 ggtttcgacc gcccccatcg ccttcgcctt gtcgaggatc gcgccgtagt cctcgcccgg
     1141 ctgccccagg ttgacggtca gcgtgaccac ctcggcgtcg taggcctgct ggatccaatg
     1201 cagcatcaca ctggtgtcga gaccacccga atagagcagc acgacacggt tgacctcttc
     1261 ggggcgggcc tcgtatgagg cgaccgggcc cgaaagcgaa tcgagtgaga tttcggtcat
     1321 ccggggccga tcccgccgct gctcgagccg ctgtcggtgc tcgtgtccgg ggtgactccc
     1381 ccggtgtcgg tgccggtgct ggtatcggga gtgacaccgc cggtatccgt gccggtaccg
     1441 gtgctggtgt cgggcgagat gccgccggaa gtgtccgtgc cggtgtcggt cggggtgacc
     1501 ccgccgtcat cttcaccgga ggaatcggtg gtgccggttt ctccactcgg ggtgaccggg
     1561 acgaccggtg tggtcacggc agtgtctccg gtggttgccg aagtgtcgtc gctgctgtcg
     1621 ccgccggaac tgatcacgat caccgcaatc accaggccca cgatcacggc gatcagcacg
     1681 atgatcaggt ttcgcaggtg cttcttctgc tcttcgggcg tgtagtgagg gtcctcgtcg
     1741 tggcgccggc ggggcggctc gtcgtttctc atcactcttg tgcggtcatc gctactcatc
     1801 ggaaatctcc agtcaattcg attcgtccgg atggtacccg gccagcgtct cgtctataag
     1861 ggcgacagcc cggtcagcct gctcatcggt aaggacaaac ggtggcagaa agcgaagggt
     1921 gtcgggtcgc ggcgcgttgg ccaccagccc gttcccgagc agggccgcat tgacctcgcc
     1981 ggcattcaaa ccgggttcca gccggacccc gatcatcaag cccctccccc gcacttccgc
     2041 caccccgtca agggcgttca gcctttccgt cagggaggcg ccgagcgcgc ggatccgccg
     2101 cagcatctcg gggtcgtcga ccaggtcgag gacggcgagc gcggcgctgg aagcgaccgg
     2161 tccggccgcg aaggtcgagc cgtgatcgcc ggcagccagg acctccccgg tctcggggtt
     2221 gccgatgcag gcgccgatcg ggtcagtgcg gttgtcgtgc gccagcaccg cga
//
LOCUS       DAOQMF010000290         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30708_length_2270_cov_3.790068, whole genome shotgun sequence.
ACCESSION   DAOQMF010000290 DAOQMF010000000
VERSION     DAOQMF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30708_length_2270_cov_3.790068"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..633
                     /locus_tag="PLV77_05645"
     CDS             253..633
                     /locus_tag="PLV77_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC15364.1"
                     /translation="MTEIIAPKVGKNSPRQAWLPDFNLAFVGGDIEKTLGFVAEDVTW
                     ELVGEGTIKGRDGMRAWLQSMAGKQARRVEFRLFITHGRSAAVNGSYEMESGSKFEFC
                     DIYEFANAKNDSPITKYTSYVVRV"
     gene            651..1142
                     /locus_tag="PLV77_05650"
     CDS             651..1142
                     /locus_tag="PLV77_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15365.1"
                     /translation="MELKPKQSDKPGKERGNDFERVHGDLLRSFLEPGESLLGVAAVN
                     WQRSMFKQTVSALGVTGSRLIIQPLDRKGKAATEAPTFIRKEEITKGSYGGGGGMGNS
                     PTSLIMDSASIEVKLKTSGGEKYKLLLMTGEGMFGGMAGGPAQRNGAEALVSFLEGAG
                     RSI"
     gene            1224..1886
                     /locus_tag="PLV77_05655"
     CDS             1224..1886
                     /locus_tag="PLV77_05655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC15366.1"
                     /translation="MALQLGQEAPNFTAETTEGTIDFHDWIGDSWAVLFSHPKDFTPV
                     CTTELGYMANAKPAFDERGVKIIGLSVDPLDSHEDWAKDIEETQGTAPNYPLISDADF
                     KIAKAYGMLAADVEGDAADRTPADNQTVRNVFVIGPDKKIKLILVYPMTTGRNFDEVL
                     RVIDSLQLTANHKVATPANWKSGDDVIIAGSVNNDQAKEIFGEWEEPKPYIRIVPDPT
                     AS"
     gene            complement(1961..2221)
                     /locus_tag="PLV77_05660"
                     /pseudo
     CDS             complement(1961..2221)
                     /locus_tag="PLV77_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
BASE COUNT          480 a          707 c          740 g          343 t
ORIGIN      
        1 gagagccgga atgatcagcc ccattacgga agccacggcc attacccctc cggcgatgcc
       61 gagcagcagg ggcacgatgg accgggcccg gttgccgagc agcatcaccg ggatcgcccc
      121 gagtccggtg gcgagggcgg tggcgctggc gaggagaatc agtgcgatca agacgactca
      181 aagcgtaggg gcgacggcgg aggcggtgac cggcccttcg ccgagcactc cttcagcgac
      241 taggctggcc gcatgaccga gatcatcgcg ccgaaggtcg ggaagaactc gccgcgtcag
      301 gcctggctgc ctgatttcaa tctcgccttc gtcggcgggg atatcgagaa gacccttggc
      361 ttcgtggccg aggacgtcac ctgggagctg gtcggcgagg ggacgatcaa gggtcgcgac
      421 ggaatgcggg cctggcttca gtcgatggcc ggcaagcagg cccggcgggt cgagttcagg
      481 ctcttcatca cccacggccg atctgccgcg gtcaacggca gctacgagat ggaaagcggt
      541 tcgaagttcg agttttgcga catctacgaa ttcgccaatg cgaagaacga cagcccgatc
      601 acgaaataca cctcgtatgt agtgcgagtc tgaaaggagc ggggcggcgc atggaactca
      661 aaccgaagca gtccgacaag ccgggaaagg agcggggaaa cgacttcgag cgggtccacg
      721 gtgatctgct caggtccttc ctcgaaccgg gcgagagcct gctcggggtg gcggcggtca
      781 actggcagcg cagcatgttc aagcagacgg tcagcgcgct gggggtgacc gggagcaggc
      841 tgatcatcca gccgcttgac cgcaagggca aggcggccac ggaggccccg actttcatcc
      901 gcaaggagga gatcaccaag ggcagctacg ggggaggtgg cggcatgggc aactcgccga
      961 cctctctgat catggactcc gcatcgatcg aggtgaagct gaagacctct ggcggcgaga
     1021 agtacaagct gctcctgatg accggcgagg gcatgttcgg tggcatggcc ggcgggccgg
     1081 cacaaagaaa cggcgccgaa gccctggtca gcttcctcga aggagccgga cgctcgatct
     1141 gacctaaata cctgtatccg gatcaggtat atgggacata ttggctatag ttggttcaca
     1201 gtcaattctt gaaggagaaa agcatggccc tacagctggg acaggaagca ccgaatttca
     1261 ctgccgaaac caccgaggga acgatcgatt tccacgactg gatcggcgac tcctgggcag
     1321 tcctcttttc gcacccgaag gacttcaccc ccgtctgcac caccgaactc ggttacatgg
     1381 cgaatgccaa gccggccttc gacgagcgcg gcgtgaagat catcgggctc tcggtcgatc
     1441 cgctcgacag ccacgaggac tgggccaagg acatcgagga gacccaggga acggccccga
     1501 actacccgct gatctccgac gccgacttca agatcgccaa ggcctacgga atgcttgccg
     1561 ccgacgtcga gggtgacgcc gccgatcgca ccccggccga caaccagacc gtccgcaacg
     1621 tcttcgtgat cggtccggac aagaagatca agctgatcct ggtctacccg atgacgaccg
     1681 gccggaactt cgacgaggtc ctccgcgtga tcgactcgct gcagctgacc gccaaccaca
     1741 aggtcgccac cccggccaac tggaagagcg gcgacgacgt gatcatcgcc ggctcggtca
     1801 acaatgacca ggcgaaggag attttcggcg agtgggaaga gcccaagccg tacatccgga
     1861 tcgtcccgga cccgaccgcc agctgatcgg atctgccgag atggcggaag tcagtcaata
     1921 gggctgactt ccgccttctc gaagcttcgc cccgcctcgg ctagaaggcg aagcggacct
     1981 ggccgccgcg cttcggcgag gaggtgatga agcgggccac ggcaggctcc tgggggccga
     2041 ccggcaaaag gatcccggcc cgggccaccg ccttcatcac gatctccatc tcggccatcg
     2101 cgaactgcat gccgatgcag cggcgaacac cgcccccgaa ggggaaccag gtgtaggtgc
     2161 cgggcttgac cccgtcccag cgctccggct tgaaggccaa cgggtcggga taaacgtcct
     2221 cgcggcgatg gacgagtctt caccgccgct cacgacgccc ggaccacccg
//
LOCUS       DAOQMF010000291         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30777_length_2267_cov_3.808318, whole genome shotgun sequence.
ACCESSION   DAOQMF010000291 DAOQMF010000000
VERSION     DAOQMF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30777_length_2267_cov_3.808318"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2038
                     /locus_tag="PLV77_05665"
     CDS             <1..2038
                     /locus_tag="PLV77_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC15367.1"
                     /translation="SEGVVVLRIADSRIVLTNPQFDEMHGFDRGELIGRNIDVIAPDD
                     LSEDEVQMRVDVLHELADKGETSYENRSVRKDGTDIWSRVNGVITTVPEHGPVLILVK
                     YDVTGERIAKSAGRDAEMRFRQVFEQSPIGLCLVTPEGRFEKVNRSFESITGYSAGEL
                     SEMTFADITHPDDLEEDVAMTGELFAGERDGFSMEKRYIRKNGEVVWIYLTVTMLRDQ
                     DGKPNQALSMVEDITERQRLSQQLKYLADHDPLTGLYNRRRFEEELSSAIDSGHGRGI
                     AILVIDLDNFKFVNDSFGHTIGDRLIVRTAELLKSRLRSDDTLARQGGDEYVVVLRDI
                     SPEDALTTAEELVRLIARDVRIVGAEHSARVTASIGVAVSSEVSPVPEETLMMQADIA
                     MYEAKDAGRNGARVFQPGEDSHVTLGIDWAGKLRSALENEELLVYAQPIHRLNGEGPP
                     HHELFVRMRDRSGSIIMPGAFLPTAERHDLVQELDSWVISKAISTLAELEGDGCPRLQ
                     INLSGRTVGDPKLPEFVQGELSRAGVDPSNLIFEVTETSAIGNMTGAQEFSARMNELG
                     CGFALDDFGTGFASFYYLKHLAFDYVKIDGEFVQNLASDPVNRLLVKALVEISKGMGK
                     RTVAEQVEDARTLEVLREFGVDFVQGHYLGRPRPLDRSELASLPALPLDTALPG"
     gene            complement(2044..>2267)
                     /locus_tag="PLV77_05670"
     CDS             complement(2044..>2267)
                     /locus_tag="PLV77_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15368.1"
                     /translation="SVPSPGAYASGAWAWKCGPDVTCPAFAAVSYSIEDGTKQVIILV
                     LASVFGALIALLVGEVLIEWARKRFREPD"
BASE COUNT          432 a          708 c          749 g          378 t
ORIGIN      
        1 gtccgagggc gtcgtggtcc tgcggatcgc cgattcgcga atcgtgctga ccaacccgca
       61 gttcgatgag atgcacgggt tcgaccgggg cgagttgatc ggccggaaca tcgatgtgat
      121 cgccccggac gatctctccg aggatgaagt ccagatgcgc gtcgacgtgc ttcacgaact
      181 ggccgacaag ggggagacca gctacgagaa ccgctcggtc aggaaggatg gcaccgacat
      241 ctggagccgg gtcaacggcg tgatcacgac cgtgccggaa cacgggccgg ttctgatcct
      301 cgtcaagtac gacgtgaccg gggaacgaat cgcgaaatcg gccgggcgcg acgccgagat
      361 gcgcttccgg caggttttcg agcagagccc gatcggtctt tgcctggtca ctcccgaggg
      421 ccgtttcgag aaggtgaacc ggagcttcga gtcgatcacc ggctactccg cgggtgagct
      481 ctcggaaatg acctttgccg acatcaccca tccggatgac ctcgaagagg acgtcgcgat
      541 gaccggcgaa ctcttcgccg gggagcggga cggcttcagc atggagaagc ggtacatccg
      601 caagaacggg gaggtggtct ggatctacct gaccgtgacc atgctgcgcg atcaggacgg
      661 caaacccaac caggcactgt cgatggtcga agacatcacc gaacgccagc ggctcagcca
      721 gcagctcaag tatctggccg atcacgaccc gctgaccggc ctctacaacc ggcgtcgctt
      781 cgaagaggaa ctcagctcgg cgatcgactc cggacacggc cgggggatcg cgatcctggt
      841 catcgacctc gacaacttca agttcgtcaa cgacagtttc ggccacacga tcggcgaccg
      901 gttgatcgtg cggacggccg agctgttgaa gagccggcta cgctccgatg acaccctcgc
      961 ccggcagggt ggcgacgaat acgtggtggt gcttcgcgac atcagccccg aagacgcttt
     1021 gaccaccgcc gaggaactgg tcaggctgat cgcccgggac gtgcggatcg tcggggccga
     1081 acattctgcc cgggttacgg ccagcatcgg cgtcgcggtt tcgtcggaag tctcgccggt
     1141 gcccgaggaa acgctgatga tgcaggccga catcgccatg tacgaagcca aggacgccgg
     1201 ccgcaacggt gccagggtct ttcagcccgg cgaggattca catgtcacgc tgggcatcga
     1261 ctgggccggg aagctgcgca gcgcgcttga aaacgaagag ctgctggtct acgcgcagcc
     1321 gattcaccgg ctcaacggag agggcccgcc gcatcacgag cttttcgtcc ggatgcgcga
     1381 tcgcagcggt tcgatcatca tgcccggcgc cttcctgccg accgccgaac gccatgacct
     1441 ggtccaggaa ctcgattcat gggtgatcag caaggcgatt tcgaccctgg ccgagctcga
     1501 aggggacggt tgcccgcggc tccagatcaa cctgtccgga cgcacggtcg gggacccgaa
     1561 gctgcccgaa ttcgtgcagg gcgagctctc gagggcgggg gtggacccgt ccaacctgat
     1621 cttcgaagtg accgagacca gcgcgatcgg caacatgacc ggggcccagg agttctcggc
     1681 ccgtatgaac gagctcggct gcggtttcgc cctggatgat ttcgggaccg gattcgcgtc
     1741 cttctattac ctcaagcatc tggcgttcga ctacgtgaag atcgacggcg agttcgtcca
     1801 gaacctggcc tcggacccgg tcaaccggct gctggtcaag gccctggtgg agatttcaaa
     1861 aggcatgggc aagcggacgg tcgccgagca ggtcgaagac gcgaggaccc tggaggtgct
     1921 ccgcgagttc ggggtcgatt tcgtccaggg ccactatctc ggccggcccc ggccgctgga
     1981 ccgctccgaa ctggcttcgc tgcccgccct gccgttggat acggcgctcc cgggctgaac
     2041 cgctcagtcc ggttcgcgga agcgttttct cgcccactcg atcagtacct cgccgaccag
     2101 cagtgcgatc aacgcgccga agacggaggc gaggaccagg atgatcacct gcttggtgcc
     2161 gtcctcgatc gagtagctga ctgcggcgaa agcggggcag gttacatccg ggccgcattt
     2221 ccaggcccag gcgccggagg cataggcccc gggcgagggg accgatg
//
LOCUS       DAOQMF010000292         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30805_length_2266_cov_3.665762, whole genome shotgun sequence.
ACCESSION   DAOQMF010000292 DAOQMF010000000
VERSION     DAOQMF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30805_length_2266_cov_3.665762"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLV77_05675"
     CDS             complement(<1..227)
                     /locus_tag="PLV77_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HNC15369.1"
                     /translation="MYRWYVINTYSGQENKVKQKLEHRIESMGQGHRVRKVVVPTEQV
                     SEVKDGQKIQVEKRTMPGYVLIQMEMTDNAW"
     gene            complement(236..751)
                     /gene="secE"
                     /locus_tag="PLV77_05680"
     CDS             complement(236..751)
                     /gene="secE"
                     /locus_tag="PLV77_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNC15370.1"
                     /translation="MAKSRAQRKAERRAREAAAQGGVESDSQAQHDTQVPKSGDIAEI
                     EAVEAGIAAGAREEDLETPDAPKGKQELSSKEEKRKARQEEKRKKSEQKKQEEQQLAK
                     RQKQAQGERKRGGVIAFLSSCIAELKRVQWPDRDTLIQATAVTVVFVLVAAVYLGLLD
                     LAFNWLVQRII"
     gene            complement(751..915)
                     /gene="rpmG"
                     /locus_tag="PLV77_05685"
     CDS             complement(751..915)
                     /gene="rpmG"
                     /locus_tag="PLV77_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNC15371.1"
                     /translation="MARGDVRIAVTLACESCKRRNYQTNKSKRNTPDRIEMRKYCRWC
                     GKHQPHKETR"
     gene            complement(1137..>2266)
                     /locus_tag="PLV77_05690"
     CDS             complement(1137..>2266)
                     /locus_tag="PLV77_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15372.1"
                     /translation="EATLTGGSLSLAALGEVELPADQLKLIGQRAANGTWSVPQSGVI
                     FEPIEQTIDAPGIGSVTVKISISATADGAGSLPTGGGAATLNLPVQAKLEASLGDVTL
                     IGPDVDCALRPVTFDLTGTYDQAGKTVSLTSPAVSFPKVSAGCGALGGTVNSLLELPR
                     DDIAITLNFALEEFGALVAPEITSGPSGETAETAASFAFTGESSATFECRIDGGAWEA
                     CTSPKDYSNLTAGAHTFEVRQIVSGGEVSDPASRTWTVTPEVSKTSNLAKPVVKAPKK
                     VKSGKLALIRVSVKNTGQAAASNVKVCLTLKQAKKLANGKAKRCKTIKTIAAGKTSVA
                     RFLVKTKKITTKTKRLSYAVTAPIGDGTSKHTKTGHVTLLK"
BASE COUNT          411 a          720 c          648 g          487 t
ORIGIN      
        1 ctccaggcgt tgtcggtcat ttccatctgg atcagcacat agccgggcat ggtccgcttc
       61 tcgacctgga tcttctggcc atccttgact tcggagacct gttcggtggg gaccacgacc
      121 ttccggacgc ggtgaccctg acccatcgat tcgatgcggt gctcaagctt ctgcttgacc
      181 ttgttttcct gaccggagta tgtgttgatt acgtaccagc gatacatgaa gttccttaga
      241 tgatcctctg gaccagccag ttgaaggcga ggtcgagaag gccgaggtag acagcggcga
      301 cgagcacgaa gaccacggtc acggcggtcg cctggatcag ggtatcccga tccggccact
      361 ggacccgctt gagctcggcg atgcaggatg acaggaatgc gatcactccg ccacgcttgc
      421 gctcgccctg ggcctgcttc tgccgcttgg cgagctgctg ctcttcctgt ttcttctgct
      481 cggacttctt gcgcttctct tcctgccggg ccttgcgctt ctcttccttg gagctcagct
      541 cctgcttgcc cttgggggcg tcgggagtct cgagatcctc ttcccgggcg cccgccgcga
      601 ttccggcctc gacggcctcg atctcggcga tgtcgccgga cttgggcacc tgggtgtcgt
      661 gctgggcctg tgaatcagac tcaacacctc cctgtgccgc ggcctcgcgc gcgcggcgct
      721 cggctttccg ctgtgcccgg gacttggcca ctagcgggtt tccttgtggg gctggtgctt
      781 gccgcaccag cggcagtact tccgcatctc gattcgatcg ggagtattgc gcttcgactt
      841 attcgtttgg tagttacgac gcttgcagct ttcacatgcg agcgtcaccg cgatgcggac
      901 atctccgcgt gccatatttc ctcgctactc aggcagtctc gttacaaagt aaagcctcgg
      961 ccaaagggca gaggcgcgtc taggaagaat accggtttag agccggatgc gagtcctccc
     1021 gtccgatctg gctgctagcg cgtggtttgc ggtcgctggc gtcagccgag ggcccggaaa
     1081 caggaagggc ccgccccgga agttccggaa cgggcccacc ttcagacggg gaccggctat
     1141 ttcagcagtg tgacgtggcc ggttttggtg tgtttgctgg tgccgtcacc gatcggtgcg
     1201 gtaaccgcgt agctcaaccg tttcgtcttg gtcgtgatct tcttggtctt gaccaggaac
     1261 ctcgcaacgg aggtcttgcc ggcagcgatc gtcttgatcg tcttgcaacg cttggccttt
     1321 ccgtttgcga gtttcttggc ctgcttgaga gtcaggcaaa ccttcacgtt cgaagcggcc
     1381 gcctggccag tgttcttgac cgaaacgcgg atcagggcga gcttgcctga tttcaccttc
     1441 ttcggggcct tcaccaccgg tttggcgaga ttgctggtct tcgatacctc gggagtgacc
     1501 gtccaggtcc ttgacgcggg atcggaaacc tcaccaccgg aaacgatttg cctgacctcg
     1561 aaggtatggg caccagcagt caggttcgag taatccttcg gtgaagtgca ggcctcccag
     1621 gccccaccat caatccgaca ctcgaacgtc gcgctcgact cgccagtgaa agcaaacgaa
     1681 gcggcggtct cagcggtctc accggacgga ccggaagtga tctccggtgc caccaaagct
     1741 ccgaactcct ccaaagcgaa gttcaaagtg atcgcgatgt catcgcgcgg caactcaagc
     1801 aaactgttga ccgtgccacc aagcgcaccg caaccggcac tcaccttcgg gaacgaaacc
     1861 gccggagagg tcaaactcac agtcttccca gcctgatcgt atgtaccggt cagatcgaaa
     1921 gtgaccggcc tgagcgcgca atcaacatcc ggaccgatca gcgtcacatc accaaggctc
     1981 gcttcgagct tcgcctgaac cggcaggttc aacgttgccg ccccgccacc tgtcggcaac
     2041 gaaccggcac catcggcagt agccgaaatc gagatcttga ccgtgaccga tccgataccc
     2101 ggagcatcga tcgtctgctc gatcggctcg aaaataaccc ctgactgcgg aaccgaccag
     2161 gtgccattag cggcccgctg accaatcagc ttcaactgat cagccggcaa ctcaacctca
     2221 ccaagcgccg ccaaagacag gctgccacca gtcaacgtcg cttcaa
//
LOCUS       DAOQMF010000293         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30925_length_2261_cov_3.300091, whole genome shotgun sequence.
ACCESSION   DAOQMF010000293 DAOQMF010000000
VERSION     DAOQMF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30925_length_2261_cov_3.300091"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /gene="rplN"
                     /locus_tag="PLV77_05695"
     CDS             <1..267
                     /gene="rplN"
                     /locus_tag="PLV77_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNC15373.1"
                     /translation="IGDVITATVKQASPQGNVKKGEVVQAVIVRTKKEIGRQDGTYIA
                     FDENAAVLIDAQNNPRGTRIFGPVARELRDRDFMKIISLAPEVL"
     gene            267..593
                     /gene="rplX"
                     /locus_tag="PLV77_05700"
     CDS             267..593
                     /gene="rplX"
                     /locus_tag="PLV77_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNC15374.1"
                     /translation="MRIRTDDTVQVISGKEKGKQGKVLRTDPKAGTVYVEDLNMIKRH
                     QRAQPSPDGRGQGKEGGIIEMEGPIHVSNVALVDPTDNKPTRIGVRITDDGVRQRYSK
                     RTGEAI"
     gene            602..1171
                     /gene="rplE"
                     /locus_tag="PLV77_05705"
                     /pseudo
     CDS             602..1171
                     /gene="rplE"
                     /locus_tag="PLV77_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932832.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            1381..1566
                     /locus_tag="PLV77_05710"
     CDS             1381..1566
                     /locus_tag="PLV77_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNC15375.1"
                     /translation="MAKTSQKVRQQRKAKFSTREYNRCRRCGRPRAYYRKFGICRICL
                     REAAHEGYIPGMTKSSW"
     gene            1571..1966
                     /gene="rpsH"
                     /locus_tag="PLV77_05715"
     CDS             1571..1966
                     /gene="rpsH"
                     /locus_tag="PLV77_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNC15376.1"
                     /translation="MLTDPISDYLTRIRNGLSSGLATVEVPSSKLKLEVSRILTEQGY
                     ISGYEKSAADVGEKITVELKYTKDHQPVITGLERVSRPGRRRYVGHGEVPRVHGGTGT
                     AIITTSHGVMTGHEAKKEGVGGEVLAYVW"
     gene            1977..>2261
                     /gene="rplF"
                     /locus_tag="PLV77_05720"
     CDS             1977..>2261
                     /gene="rplF"
                     /locus_tag="PLV77_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNC15377.1"
                     /translation="MSRIGKKPIDLPDGVEIDIKPGLITVKGPKGELTQEVSTDMTVK
                     TAEGVISVERPTNRPNHRALHGLTRSLIANMIVGVTDGYTKVLEIQGVGYR"
BASE COUNT          491 a          744 c          702 g          324 t
ORIGIN      
        1 atcggtgacg tgatcaccgc gaccgtcaag caggccagcc cgcagggcaa cgtcaagaag
       61 ggcgaggtcg tccaggccgt aatcgtccgg accaagaagg agatcggccg ccaggacggc
      121 acctacatcg ccttcgacga gaacgctgcc gtgctgatcg acgcgcagaa caacccgcgc
      181 ggtacccgca tcttcggacc ggtcgcccgc gaactgcgtg accgcgactt catgaagatc
      241 atctcgctcg ccccggaggt gctgtgatgc gtatccgcac cgacgacacc gtgcaggtaa
      301 tcagcggcaa ggagaagggc aagcagggca aggttctgag gaccgacccc aaggccggaa
      361 ccgtgtacgt tgaagacctc aacatgatca agcgccacca gcgcgcccag ccctcgccgg
      421 acggccgggg ccagggcaag gaaggcggga tcatcgagat ggagggtccg atccacgtgt
      481 cgaacgtggc cctggtcgac ccgaccgaca acaagccgac ccgaatcggc gtccgcatca
      541 ccgacgacgg cgttcgccag cgctactcga agcgcaccgg cgaggccatt tagagaagat
      601 catggaagca gctacgacaa cacaacaggc agtgccgccg ccgcggctcc gggacaccta
      661 catcaacgag gttctcccga agctgatgga gaccttcggc tactcgactc cgatgcgggc
      721 cccgcgcctt cagaagatca ccctgaacat gggcctcggc tcctcggttg actccaagtc
      781 ccgcgaaaag gcgatcgaag agctcgccac gatcgccggt cagatgccga acacccggat
      841 cgccaagaag tcaatcgcgt ccttcaagct tcgcgaggga atgccggtcg gcgcctcggt
      901 gaccctccgg ggcgcccgga tgtgggagtt cctcgatcga ctgaactcga tcgcgatccc
      961 gcgcatccgt gacttccgcg gactcaaggc gacctcattc gacggtcgcg gcaactactc
     1021 catgggtgtc aaggaacagc tgatcttccc cgagatcgat tacgactcgg ttgacgagac
     1081 ccgcggtctt gacatcacga tcaccacgtc cgcaccgacg gactacgagg ctttcgagct
     1141 gctgctcggc ctgggcatgc ctttcgccaa ggaaggccgt ccggtccccg agggtgccga
     1201 agaaagcacc gccccggatg agccggctgc ggaggcaact gatggagccg gctccaccga
     1261 agcgccggcc gaagaagagg ccccggcaga agcagcagac actgaaaatg acgcggccga
     1321 ggaggcatcg gacgatgccg ccgaagacgc agccgccgaa gagcaggagg accaggccta
     1381 atggccaaga cgtcacagaa ggtgcgccag cagcgcaagg cgaagttttc aacccgcgag
     1441 tacaaccggt gccggcggtg cggccgcccc cgtgcgtact accgcaagtt cggcatttgc
     1501 cggatctgcc tgcgcgaggc cgcccatgag ggctacatcc ccggcatgac gaagtcgagc
     1561 tggtagtcaa atgcttaccg atccgatttc cgactacctg acccgcatcc ggaatggcct
     1621 cagctccggc ctggccaccg tcgaggtgcc cagctcgaag ctgaagctgg aggtctcccg
     1681 catcctcacc gagcagggct acatctcggg ctacgagaag agcgctgccg acgtgggcga
     1741 gaagatcacg gtcgagctca agtacacgaa ggaccatcag ccggtgatca ccggactcga
     1801 gcgggtttcc cgccccggcc gccgtcgtta cgtcggccac ggcgaggttc cccgggttca
     1861 cggcggcacc ggcaccgcga tcatcaccac ctctcacggt gtgatgaccg gtcacgaagc
     1921 caagaaggaa ggcgtcggcg gcgaggttct cgcctacgtc tggtaacgag gaattcatga
     1981 gtcgaattgg aaaaaagccg atcgatctcc ccgatggggt cgagatcgac atcaagcccg
     2041 gactgatcac ggtcaagggc ccgaagggcg agctcaccca ggaagtctcg accgacatga
     2101 ccgtgaagac ggcggaaggc gtgatctcgg tcgagcgacc gacgaaccgc cccaaccatc
     2161 gtgcgctcca cggcctgacc cgcagcctga tcgccaacat gatcgtcggc gtcaccgatg
     2221 gctacaccaa ggtcctggag atccagggcg tcggttaccg t
//
LOCUS       DAOQMF010000294         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30952_length_2260_cov_3.558277, whole genome shotgun sequence.
ACCESSION   DAOQMF010000294 DAOQMF010000000
VERSION     DAOQMF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30952_length_2260_cov_3.558277"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLV77_05725"
     CDS             complement(<1..635)
                     /locus_tag="PLV77_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC15378.1"
                     /translation="MAEVEAGLLAAVEGETGLAGDAVRTLNAGGKRMRPMLVLLCAGP
                     EAGPAAVRSGIAVELIHMASLVHDDVLDRAPLRRGLPTVYASSGRERATALGDRLFST
                     AFKVLVDGGDSAAVSEVSATAVDLARGELRQREGAFDMELTETDYFDRCALKTGRLFE
                     AACVLGRGSTGGDPGPVGLFGHRIGLAFQLLDDVLDIAGPPERTGKARGTDL"
     gene            complement(741..1463)
                     /gene="ubiE"
                     /locus_tag="PLV77_05730"
     CDS             complement(741..1463)
                     /gene="ubiE"
                     /locus_tag="PLV77_05730"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNC15379.1"
                     /translation="MNEAPPRRGDQEFAGQVNSMFDRISGVYDRMNRVMTAGLDQSWR
                     ARAAERARLEPGMKALDLCCGTGDLALMLAERVGPEGEVTGADFSRPMLDIALEKAKS
                     EGLRQARFKWADALDLPFEDESFDALTIAFGARNLADLDRGISEMRRVLVPGGRLVIL
                     EITQPKRQPLAGFFALWFDRIVPQLGKLAGDSSAYTYLPESVRSFPDAETLAGKLDAG
                     GLRHVRWTLMAGGIIALHSGVR"
     gene            complement(1464..2177)
                     /locus_tag="PLV77_05735"
     CDS             complement(1464..2177)
                     /locus_tag="PLV77_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC15380.1"
                     /translation="MNAREKEEYLREYEILKKKGKPFFPYAIMKDSVMMLIVAGVLVA
                     MSIIMGAEQGPKADPTTTTYTPRPEWYFFYLFELLRVIKPPALVPVATIGIPTICMVL
                     LLLLPFYDRGPQRVPWKRPIATTTGIMVIIAMALLTYKGAVAGSPSEIELATSPQYEK
                     GKAIVAESGCLACHAIGENGAHGGLGPDLTDIGSKIPKEAIKRSVAVGPGIMPSFQGL
                     GEENLNAVADYLASLQTEH"
BASE COUNT          352 a          765 c          775 g          368 t
ORIGIN      
        1 aggtcggtgc cccgggcctt gccggttcgc tccggcgggc cggcgatgtc gagcacgtcg
       61 tcgagcagct ggaaggcgag accgatccgg tggccgaaga gcccgaccgg ccccggatca
      121 ccgccggtgc tgccccggcc gagcacgcag gccgcttcaa agagccggcc ggtcttcagg
      181 gcgcagcggt cgaagtagtc ggtctcggtg agctccatgt caaaggcccc ctcgcgctgc
      241 ctgagctcgc cgcgggcgag gtccaccgcg gtcgcgctga cctcggagac ggcggcggaa
      301 tcacctccgt cgaccaggac cttgaatgcc gtcgagaaca gacggtcacc cagggcggtc
      361 gcccgttccc ggcccgagct ggcgtagacg gtgggcagtc cgcgccgtag cggcgcccgg
      421 tcgagcacgt catcgtggac cagactggcc atgtgaatca gctcgaccgc gatccccgaa
      481 cggaccgcag ccgggcctgc ctccgggccg gcgcaaagca ggaccagcat cgggcgcatg
      541 cgcttgccgc ccgcgttgag ggtgcggacc gcgtccccgg ccagcccggt ctccccttcg
      601 accgcggcca gcagtccggc ttcgacctcc gccatgcggg ccggtagcca gtccccggcg
      661 gtgtcggtta cagcggtgac cggctccggg agcgaccggt tccggggggc ttcgtcagcc
      721 gcgcccatgg ccgagctatc tcaccggacc ccggagtgaa gggcgatgat cccgcccgcc
      781 atcagggtcc agcggacatg ccggaggccc ccggcgtcaa gcttgccggc cagggtctcc
      841 gcatcgggga aactgcgcac cgattccggc aggtaggtgt aggcggagga gtcacccgcg
      901 agcttgccga gctgaggcac gatccgatcg aaccagaggg caaagaagcc ggccaggggc
      961 tggcgcttcg gctgggtgat ctcgaggatg accaggcgac cgcccggcac caggacccgg
     1021 cgcatctcgc tgattccccg gtcgaggtca gccaggttgc gggcgccgaa ggcgatcgtc
     1081 aaggcgtcga aggattcatc ctcgaagggc aggtcgaggg cgtcggccca cttgaaccgt
     1141 gcctgcctca gcccttcgct tttggctttc tcaagcgcaa tgtcaagcat cgggcgggag
     1201 aagtccgcgc cggtcacttc accttccggg ccgacccttt cggccagcat cagggcgagg
     1261 tcaccggtgc cgcagcaaag gtcgagcgcc ttcatgccgg gttcgagccg ggccctttcg
     1321 gccgcgcgcg cccgccagct ctggtcgagg ccggcggtca tcacccggtt catccggtcg
     1381 tacacaccgc tgatgcgatc gaacatcgag ttcacctgtc cggcgaactc ctggtcacct
     1441 cggcggggag gtgcctcgtt catttagtgc tcggtctgga gcgaggccag gtagtcagcg
     1501 accgcgttca ggttctcttc accgagaccc tggaaggagg gcatgatgcc cgggccgacc
     1561 gcgaccgaac gcttgatcgc ttccttcggg atcttcgacc cgatgtcggt caggtccgga
     1621 ccgagaccgc cgtgggcccc gttctcgccg atcgcgtggc aggccagaca gccggattcg
     1681 gcgacgatcg ccttgccctt ctcgtattgc ggcgaggtgg cgagttcgat ctcgctgggc
     1741 gagccggcca ccgctccctt gtaggtcagc agcgccatcg cgatgatcac catgatgccg
     1801 gtggtggtcg cgatcggacg cttccacggc acccgctggg gtccacggtc atagaagggc
     1861 agaagcagca gcaggaccat gcagatggtc gggatgccga tcgtggcgac cggaaccagg
     1921 gccggcggct tgatcacgcg cagcagctcg aagaggtaga agaagtacca ctcggggcgc
     1981 ggcgtgtagg tggtggtggt cgggtccgcc ttgggcccct gttcggcacc catgatgatc
     2041 gacatcgcga ccagcacgcc ggcgacgatc agcatcatca ccgagtcctt catgatcgcg
     2101 taggggaaga acggcttgcc cttcttcttc aggatctcgt actcgcggag gtactcctcc
     2161 ttttcacggg cgttcacgag ttcacctccc cgtcgccatc gtcctcggag ggtgcgtcag
     2221 ccggatctgc cggcggctcc tcgggagcgg tttcgtcagc
//
LOCUS       DAOQMF010000295         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_30967_length_2260_cov_2.524263, whole genome shotgun sequence.
ACCESSION   DAOQMF010000295 DAOQMF010000000
VERSION     DAOQMF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30967_length_2260_cov_2.524263"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLV77_05740"
     CDS             complement(<1..683)
                     /locus_tag="PLV77_05740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC15381.1"
                     /translation="MSSELEVVVVSHGAAGLLRNCLRSLRENPPAVPTGVTVADSGSP
                     DDTPDMVEREFPEVRLIRMANVGFSAANNEALEDAVNDPGCRMILLLNPDTEVRPGTI
                     DAAVERLDSDSRIGMVGVRLVTADGSFDHASKRSFPTPLAALAHFTGLGRMPGAPERL
                     SQYRATWLDENEPGPVDAVNGAFMLVRREAVREVGPLDTGYWLYMEDLDWCRRFNDAG
                     WTVFFEPAG"
     gene            complement(680..2107)
                     /locus_tag="PLV77_05745"
     CDS             complement(680..2107)
                     /locus_tag="PLV77_05745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNC15382.1"
                     /translation="MLEGQDAARPATIDQVRQLQRFRRWGLAIADATAIGIGVLVADQ
                     WSSLNSGETIWALLAIPIWIFVAKVDNLYDRDPRRIRHSTLDELPELLATAAITIAAA
                     WAITGMLDAHLTSSVMVVIGTVAFFVAILLRSLVRWLYRQVAPPERTLVVGSGPKARL
                     VSNRLIVQGGDHLDLVGYVGDSEGGDVSGPASAGARYFGRPEHLEAIAADQRVNRVVI
                     ADDDLSSRDAGEIIAACRAARVSVTMVPANQTVLGPGTELNRIAEVPMLDFQFGDPPR
                     STMAIKRAMDLIVSVSMLTLTAPFLALAAVMIRLDSPGPVFFRQVRIGRDGTHFTMYK
                     LRTMTADAEEQLDSLIDLDALDEPAFKIANDPRVTRIGRWLRRSSFDEVPQFINVLKG
                     DMSLVGPRPEEEAVVALYDERQRQRLRVKPGLTGPMQVAGRGGLTFEERLALERDYVE
                     NLTITGDIRILLRTPRAVIHGEGTF"
BASE COUNT          383 a          742 c          753 g          382 t
ORIGIN      
        1 tcgcccgccg gttcaaagaa gaccgtccag cccgcatcgt tgaaacgccg gcaccagtcg
       61 aggtcctcca tgtagagcca gtagcccgta tccagcgggc cgacctcacg gacggcttcc
      121 cggcggacca gcatgaaagc gccgttcacc gcgtcgaccg ggcctggttc gttttcatcc
      181 agccaggttg cccggtactg gctcagccgc tccggtgcgc cgggcatgcg tccgaggccg
      241 gtgaaatgtg ccagcgccgc aagtggcgtg gggaaggacc gcttgctggc gtggtcgaag
      301 gaaccgtcgg cagtgaccag gcggactccg accatgccga ttcgcgaatc ggagtcgagg
      361 cgctcgaccg cggcgtcaat cgttcccggc cggacttcgg tatccgggtt gagaagcagg
      421 atcatgcggc agcccgggtc gttcacggca tcttcgagag cttcgttgtt ggccgcggag
      481 aatccgacgt tggccatccg gatcagccgg acttccggaa attcccgttc gaccatgtcc
      541 ggcgtgtcat ccggagagcc ggaatcggca acggtcaccc cggtcggcac cgccggcggg
      601 ttttctctca gggaacggag gcagtttcga agcaggccgg cggccccgtg actgaccacc
      661 accacctcga gttcggaact caaaaagtgc cttcgccgtg aatcacggcg cgcggggtgc
      721 ggagcaggat cctgatgtca ccggtgatgg tcaggttctc gacgtaatcc cgctccaggg
      781 ccagcctttc ctcgaaggtg agacccccgc gcccggccac ctgcatcggt ccggtcaggc
      841 ctggcttgac ccgcagccgc tgccgttgcc gctcgtcgta gagggcgacc accgcctctt
      901 cctccgggcg gggcccgacc agtgacatgt cgcccttcag gacgttgatg aactgcggca
      961 cctcgtcgaa gctggaccgg cgcagccacc tgccgatccg ggtgacccgc gggtcgttgg
     1021 cgatcttgaa ggcgggctca tcgagcgcgt cgagatcgat cagcgaatcg agctgttcct
     1081 cggcgtcggc ggtcatcgtc ctcagcttgt acatcgtgaa atgcgtgccg tcgcgcccga
     1141 tcctcacctg gcggaagaac acgggccccg gcgagtccag cctgatcatc accgcggcaa
     1201 gggcgaggaa cggcgcggtg agggtcagca tcgaaaccga aacgatcagg tccatcgccc
     1261 gtttgatcgc catggtcgaa cggggcgggt cgccgaactg gaagtccagc atcggcactt
     1321 cggcgatccg gttcagttcg gtcccgggcc cgagcacggt ctggttggcc gggaccatgg
     1381 tgaccgagac ccgggcggcc cggcaggcgg cgatgatctc gccggcgtcg cgcgagctca
     1441 ggtcgtcatc ggcgattacg acccggttga cccgctggtc ggccgcgatc gcctcgaggt
     1501 gttcgggccg gccgaagtac cgggcgccgg cactggccgg tccggagacg tcgccgcctt
     1561 ccgaatcgcc gacgtacccg accaggtcga gatgatcgcc gccctgaacg atcaaccggt
     1621 tggacaccag cctcgctttc ggtcccgaac ccacgaccag ggtccgttcc ggcggggcga
     1681 cctggcggta cagccagcgg accaggctgc ggagcaggat cgccacgaag aaggccacgg
     1741 ttccgatcac gaccatgacc gaactggtca ggtgcgcatc gagcatgccg gtgatcgccc
     1801 atgccgccgc gatcgtgatc gccgccgtgg ccaggagctc cggcagttcg tcgagggtcg
     1861 aatgccggat ccgccgcggg tcgcggtcat agaggttgtc gaccttggcc acgaagatcc
     1921 agatcgggat cgccagcaat gcccagatcg tttcgccgct gttcagcgaa ctccactggt
     1981 cggcgaccag gacgccgatg ccgatcgccg tcgcgtcggc gatcgccagt ccccagcgac
     2041 ggaaacgctg cagctggcgg acctgatcga tggtggccgg ccgcgcagca tcctgacctt
     2101 ccaacacttc ggaggtagct gattctccgt gttcggttct ggagccggac tgagtcactg
     2161 gttattcaat tctcccagac caataaacac gaaacagacg gatgggtgcg gtttcccggt
     2221 ccgggtgggc gggggcgtag catcaggggt ttagcgatgc
//
LOCUS       DAOQMF010000296         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31030_length_2257_cov_3.261126, whole genome shotgun sequence.
ACCESSION   DAOQMF010000296 DAOQMF010000000
VERSION     DAOQMF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31030_length_2257_cov_3.261126"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..591)
                     /locus_tag="PLV77_05750"
     CDS             complement(163..591)
                     /locus_tag="PLV77_05750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNC15383.1"
                     /translation="MPDVTLPESPFDNLLGTEWVSSDPDQAIVRLTMRDDHRQPMAIM
                     HGGVMASLVESVCSMATARAVLPEGRIAMGQNISVNLLRPISSGEVEVVATAVHRGRM
                     TWVWRAEVKDDEGRVCAISQMTMAVREAPEGADLSQFVKK"
     gene            698..1750
                     /locus_tag="PLV77_05755"
     CDS             698..1750
                     /locus_tag="PLV77_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15384.1"
                     /translation="MPLSEDSRALLQLLLGRGKNYADISGLLGIDEAEVRSRAGTALA
                     ELDDSNRPPDPELTDYLLGQADPIGRADVGNRISSDPALAERAESITDQLRLLVPGAE
                     LPRPGSAEGGKPKPATKPPAPVAAAAPETGPAADPAGAQGRSGGFSSITSHQRRLIAV
                     LLGAALLAAVVILLVTGVFGGGSGDDEKNGPEAAPTVAVLQPVGNEQGSGQVQFGFSG
                     TSLAANVDISGLQPNEKGQGYVIWLYGSTGAFPIYAQKVGKSGAITGQIGPLNNAVIC
                     LIASDFFPTLRVSRADNDQFDQALKDASPTKNSKVKLPEYTGQTVLEGAISMPQKAKD
                     TIVPVCNGTANQSQSG"
     gene            complement(1753..>2257)
                     /locus_tag="PLV77_05760"
     CDS             complement(1753..>2257)
                     /locus_tag="PLV77_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC15385.1"
                     /translation="GKGWEKGRFHGPYLREALLERGVLVETLETAQQWSGHRNLYETV
                     RAALKSELAANGMEGIVMCHLSHAYRDGASLYFTVISTQGPDGGAANWPKVKRAACAA
                     IQSAGGTLSHHHATGRDHAPWIEGEIGTSGIEALAALKERFDPSGIMNPGKLLDASRA
                     GSDPPSR"
BASE COUNT          383 a          784 c          730 g          360 t
ORIGIN      
        1 aggcttcggc cgcgaccgcc ggatgccgat tacgaaccgt taccgcccct ggacggccgg
       61 caacgattcg gcataagcaa gtcgggcggg gtccgacccg ccctccatgt caaacccgcc
      121 cgccaacaga gacattcaag ggatgggcgg gctctgactg agctacttct tcacgaactg
      181 gctgaggtct gcgccttcgg gggcttcgcg gacggccatg gtcatctgtg agatcgcgca
      241 gacacgcccc tcgtcgtcct tgacttcggc ccgccagacc caggtcatgc ggccccggtg
      301 aaccgcggtg gcgactacct cgacctcacc gctcgagatc ggtcgcagca ggttgaccga
      361 gatgttctgc cccatggcga tccttccctc gggcagcacg gcccgggccg tggccatcga
      421 gcagacgctc tcgaccaggc tggccatgac cccgccgtgc atgatcgcca tcggctgccg
      481 atggtcgtca cgcatggtca ggcggacaat ggcctgatca gggtcggagg aaacccactc
      541 ggtgccgagc aggttgtcga acggggattc gggaagcgtt acgtcgggca tcggccggga
      601 tcatacgggt ccgaaccgga ggcccggtga acccggcttc ttcacagggt ccactcagga
      661 aacagccaac cgcgccgaac ccgtagggtt caccgtcatg ccgttgagtg aggacagccg
      721 ggcgctgctg cagctgctgc ttggccgagg gaagaattac gccgacattt cgggcctgct
      781 ggggatcgac gaggccgagg tgcgcagccg cgccggcacc gcgctggccg aactggatga
      841 ctcgaaccgg cccccggacc cggaactcac cgactatctg ctcggtcagg ccgatccgat
      901 cggccgggcc gacgtcggca acaggatttc atcggacccc gccctggccg aacgggcgga
      961 gtcgatcacc gatcagctca ggttgctcgt ccccggggcc gaactgccgc gtccgggatc
     1021 ggccgaaggt ggcaagccga agccggcaac aaagcccccc gcaccggtcg ctgccgcggc
     1081 gccagagacc gggcccgccg cagacccggc cggcgctcag ggtcgctccg gcggtttctc
     1141 ctcgatcacc agtcaccagc gacgcctgat cgcggtgctg cttggcgccg ccctgctggc
     1201 cgcggtcgtg atccttctgg tgaccggggt cttcggcggc ggctccggcg atgacgagaa
     1261 gaacggcccc gaggccgcac ccaccgtcgc ggtactccag ccggtcggca acgaacaggg
     1321 ctcgggccag gttcagttcg gcttctccgg aacttcgctg gcggccaacg tggacatctc
     1381 cggacttcag ccgaatgaaa agggccaggg ttacgtgatc tggctttacg gctcgaccgg
     1441 tgcctttccc atttatgccc agaaggtcgg caagtccggg gcgatcaccg gccagatcgg
     1501 accgctcaac aacgcggtga tctgcctgat cgcctccgat ttcttcccca ccctgcgggt
     1561 gtcccgagcc gacaacgatc agttcgatca ggcgctcaag gacgccagcc cgaccaaaaa
     1621 cagcaaggtc aagcttcccg agtacaccgg ccagaccgtg cttgaaggcg cgatctccat
     1681 gccccagaag gccaaggaca cgatcgtccc ggtctgcaac ggcaccgcaa accagagcca
     1741 gtcgggatag cgctaccggg agggcgggtc cgaccccgcc cggctcgcat caagcaactt
     1801 gccggggttc atgatgccgg acgggtcgaa gcgttccttg agggcggcca gggcctcgat
     1861 tccgctggtt ccgatctccc cctcgatcca gggcgcatgg tcacggccgg tggcgtggtg
     1921 atgtgagagc gtccccccgg ccgactgaat agccgcgcag gccgcacgct tcaccttcgg
     1981 ccagttggcc gccccgccgt ccggcccctg ggtggagatc accgtgaagt agagcgaagc
     2041 gccgtcgcgg taggcatgcg agagatggca catgacgatg ccttccatcc cgttcgcagc
     2101 cagttcgctc ttcagggccg cgcggaccgt ctcgtagagg ttcctgtggc cactccattg
     2161 ctgggcggtc tcgagcgtct cgaccaggac gccccgctcc agcagggcct cgcgcaggta
     2221 cggcccgtgg aagcggccct tctcccagcc cttgccg
//
LOCUS       DAOQMF010000297         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31104_length_2254_cov_3.941792, whole genome shotgun sequence.
ACCESSION   DAOQMF010000297 DAOQMF010000000
VERSION     DAOQMF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31104_length_2254_cov_3.941792"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLV77_05765"
     CDS             complement(<1..275)
                     /locus_tag="PLV77_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNC15386.1"
                     /translation="MSEEVQAPTSPAGNKGESEFVEYSRARQSMSRRIAESKATIPHI
                     YLQVEADVTALVRRRAALRAEGETATINDLVIKAAALALRNHSRVNST"
     gene            complement(331..1617)
                     /locus_tag="PLV77_05770"
     CDS             complement(331..1617)
                     /locus_tag="PLV77_05770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNC15387.1"
                     /translation="MAEIVMPRLTDSMEEGVILAWLKADGDEISVGDELVEIETDKAS
                     MVYESDLAGTLEILVPEGETRPIGEPIANVGDGSSSRGGGAVAVGDPVGRTSPGVPPG
                     PPTATASSSEEGSPDLRETSEDSPSMPSVSDGESNSGERIKASPLARRIAEDLGVDLT
                     GLSGSGPGGRIIRVDVEGAASGASEETARKPDQAPVDSAKGTPEIVELNRTQQLIARR
                     MSESKATIPHFYLRAVVDMDRAVEVRAAFKEEAADNETVPSLNDFVVKAAAIALKRHP
                     LANAAFRDGHFELYPKVNVGIAVATEGSLIVPVIKDADHLTLTEISDETRRLAGRVRS
                     GEVTPPELAGGTFTVSNLGMFGVSGFDAVINPGQAGILSVGEVTERPVVRDGKIASAR
                     LMEITLACDHRILYGAEGAEFLASVKTNLEEPGLLA"
     gene            complement(1617..>2254)
                     /locus_tag="PLV77_05775"
     CDS             complement(1617..>2254)
                     /locus_tag="PLV77_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNC15388.1"
                     /translation="HSFEALSLQVPGLLVACPSTPADAKALLKASIRDENPVIFIEHE
                     SLYGIKGEVPEGEDEILRFGEAAIRREGSDVTIVGILKMAHVAERAAAELEEEHGISA
                     EVIDPRTLRPLDLDTILASVKKTNRAVIVEEGWPHGGVGANLSTLITEQAFDYLDAPV
                     QRVTGADVHMPYSRRMEQAAIPHEENVIEAVLATTEGAFPPAGGNSGEGDS"
BASE COUNT          344 a          755 c          736 g          419 t
ORIGIN      
        1 gtgctgttga cccggctgtg gttgcggagg gccagcgccg ccgccttgat caccaggtcg
       61 ttgatggtcg ccgtctcgcc ttcggcccgg agggccgccc ggcggcggac cagtgcggtg
      121 acgtcggcct cgacctgcag gtagatgtgg gggatggtcg ccttcgactc ggcgatccgc
      181 cgcgacatcg actgtcttgc ccgtgagtac tcgacgaact cgctttcgcc cttgttgccg
      241 gccggactgg tgggtgcctg tacctcttcg ctcatctgga gtgacgatat gggcatccgg
      301 actccggtgt cggccccgcg gcgcccgtct tcaggcgagc aggccgggtt cctcgaggtt
      361 cgtcttgacc gaggcaagga attcggctcc ctcggccccg tagaggatgc ggtgatcaca
      421 ggcgagcgtg atctccatca ggcgggcgga ggcgatcttt ccgtcccgca cgaccggacg
      481 ctcggtgacc tctcccaccg agaggatgcc cgcctgcccg gggttgatca ccgcgtcgaa
      541 tccgctcacc ccgaacatgc cgaggttcga gacggtgaag gtgccgccgg ccagctcggg
      601 aggcgtgacc tcgccggagc ggacccgccc ggcaaggcgc cgcgtttcat cgctgatctc
      661 ggtcagggtc aggtggtcgg cgtcctttat caccggcacg atcagcgaac cctcggtcgc
      721 aactgcgatc ccgacgttga ccttggggta gagctcgaag tggccgtccc ggaaggcggc
      781 gttggccagc ggatgccgct tcagggcgat ggccgcagcc ttgaccacga agtcattcag
      841 cgaggggacg gtctcgttgt cggcggcttc ttccttgaag gcggccctga cctcgaccgc
      901 gcggtccatg tcgaccacgg cgcggagata gaagtgaggg atggtcgcct tcgactccga
      961 catgcgccgc gcgatcaact gctgggtccg gttcagctcg acgatctcgg gcgtcccctt
     1021 ggccgagtca accggggcct ggtccggttt cctcgcggtc tcctccgacg ccccgctcgc
     1081 cgcgccttcc acatcaaccc tgatgatccg acccccggga cccgaccccg aaagaccggt
     1141 cagatctact cccaaatcct ccgcgatacg tcttgccaga ggcgaagctt tgatccgttc
     1201 acccgagttg gactcaccat cggaaacaga aggcattgaa ggggaatctt ctgaggtctc
     1261 gcgcaggtct ggcgaaccct cttcggagct ggaagcggtg gccgttgggg gtcccggcgg
     1321 aactcccggc gaagtccggc cgacgggatc cccaacggcc accgcgccac caccacggct
     1381 tgaagatccg tcaccaacat tcgcgatcgg ttccccgatc gggcgggttt cgccttcggg
     1441 gacgaggatc tcaagggttc cggcgaggtc ggattcgtag accatcgagg ccttgtcggt
     1501 ctcgatctcg accagctcgt caccgaccga gatttcgtcc ccgtcagcct tcagccaggc
     1561 gaggatcacg ccctcttcca tcgagtcggt caggcggggc atgacgatct cggccatcag
     1621 gaatcacctt ccccggagtt accgccggcg ggcggaaatg ccccttcggt cgtcgccaga
     1681 accgcctcga tcacgttttc ctcatgggga atcgccgcct gttccatgcg gcgggagtag
     1741 ggcatgtgga cgtcggcgcc ggtcacccgc tggaccggag cgtccagata gtcgaaagcc
     1801 tgctcggtga tcagggtcga aaggttcgcg ccgaccccgc cgtgcggcca gccctcctcg
     1861 acgatcaccg cgcggttggt cttctttacc gacgcgagga tcgtgtcgag atcaagcggc
     1921 ctgagggtcc gggggtcgat cacctcggcc gagatcccgt gctcttcctc gagttccgcc
     1981 gccgcccgtt cggccacgtg ggccatcttg agaatgccga cgatggtcac gtcgctgcct
     2041 tcgcggcgga tcgccgcctc gccgaaccgg aggatttcat cctcgccttc cgggacctcg
     2101 cccttgatcc cgtaaaggct ctcgtgctcg atgaagatga ccgggttctc gtcccggatc
     2161 gacgccttga gcagggcctt ggcgtcggcc ggcgtggacg ggcaggcgac cagaagtccc
     2221 ggcacctgga gggagagggc ctcgaaactg tgtg
//
LOCUS       DAOQMF010000298         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31207_length_2250_cov_3.401367, whole genome shotgun sequence.
ACCESSION   DAOQMF010000298 DAOQMF010000000
VERSION     DAOQMF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31207_length_2250_cov_3.401367"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PLV77_05780"
     CDS             complement(<1..1052)
                     /locus_tag="PLV77_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC15389.1"
                     /translation="MAMSARDDKEELALRKRTLDSLPSAVITVDSDGIVVCVNRTAEE
                     MVPTIGPGVKLRASLEELTNVEKVDRILFHREIATFAARPEGPELHWMLWDASEPEGE
                     FVLTVWDTDWSEVINERRVAFTMAASHELRGPLTTIRGFSEILNMQPESLSPEQAEAA
                     RIVEDSARLLSVLVDDIFDLTRSSFGELRLDLHEIDLNQVIAVVISSLEPGISDRGQT
                     LTLDAEELPVIEADESRMIQMISNLVSNASVHNAAGVKIRVKARRSGNEIAITVADDG
                     VGLPFDDPEAVFLSFRRGEAASDGDRTGSGIGVPLTKLLVELHRGRITVETGRGEGTS
                     FTLWLPVDREAALAEGT"
     gene            1104..2141
                     /locus_tag="PLV77_05785"
     CDS             1104..2141
                     /locus_tag="PLV77_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNC15390.1"
                     /translation="MSPQTDGIEHGPLEDWFRERVAELEPPLDYEKIHGGMSNLTYLV
                     TDQGGSRWVLRRPPLGKVLGSAHDMGREVRVVRALAGTGVPVPPVVGYCEDEAVTGAP
                     FYVMEFVEGPVIRGPEQAAAFPSEEQRRRLGEGLVETMAKIHSVDVNEVGLSDLGRHD
                     GYVQRQLKRWSGQWEKSKTRELPLVEDVHSRLVELAPPQSAVTLVHGDYRLDNVIYSP
                     TGEVAAVVDWELCTIGEPLADLGLLMVYWREAGDEQIPLITPATVEPGFPTRAELADL
                     YAEITGRDISKLDYFVALGFWKLAIIAEGVLARFAAGQYGDDIGDVVERSQATIDLLV
                     GQADEAINRIA"
BASE COUNT          387 a          720 c          726 g          417 t
ORIGIN      
        1 gttccctcgg ccagggccgc ttcacggtcg accggtagcc acagcgtgaa cgaggtgcct
       61 tcgccccggc cggtctcgac cgtgatccgg cctcgatgaa gttcgaccag aagcttggtc
      121 aggggaactc cgatcccgga gccggttcgg tctccgtcac ttgcggcttc gccccggcgg
      181 aaactgagaa aaacagcctc ggggtcatcg aatggaaggc cgacgccgtc atcggccacg
      241 gtgatcgcga tctcgttccc ggaccgtcgg gccttgaccc ggattttcac cccggccgca
      301 ttgtgaaccg atgcgttgct gaccagattc gaaatcatct ggatcatccg gctctcgtcg
      361 gcttcgatca ccgggagttc ttcggcatcc agagttagcg tctgtccgcg gtcgctgatt
      421 ccgggctcga gtgagctgat gactaccgcg attacctggt tgaggtcgat ctcatgcaga
      481 tccagacgca gctctccgaa actgctcctg gtcaaatcga agatgtcatc gaccagaacc
      541 gacaggagac gggcggaatc ctcgacgatg cgggccgcct cggcctgttc cggactaagc
      601 gactcgggct gcatgttgag aatctccgag aacccgcgaa ttgtggtcag gggaccgcga
      661 agttcatgcg aggcggccat cgtgaatgcg acgcggcgtt cgttgatcac ttcgctccag
      721 tcggtgtccc agaccgtcag cacgaattcc ccctccggtt cggaagcgtc ccagagcatc
      781 cagtggagtt cgggcccctc cggccgggcg gcgaaagtcg cgatctcgcg gtgaaagaga
      841 atccggtcaa ccttctcgac gttggtcagc tcttccagcg aagccctgag cttcacgccg
      901 ggaccgatcg tggggaccat ctcttccgcg gtcctgttga cgcaaaccac gatgccgtcg
      961 ctgtcgacgg tgatcaccgc gcttggcaaa gaatcgagcg tgcgcttgcg gagggccagc
     1021 tcttccttgt cgtccctcgc actcattgcc atggggagag cctagccgcc atcccggtgc
     1081 actgactcgt aatctcttgt gccatgagtc ctcaaaccga tggcattgag cacggtccgc
     1141 tggaagactg gttccgggaa cgggttgccg aactcgagcc tcccctcgat tacgagaaga
     1201 tccacggcgg catgtcgaac ctcacctatc tggtcaccga ccagggcggg tcgaggtggg
     1261 ttctccgccg tcccccgctg ggcaaggttc tcggctcggc ccacgacatg ggccgcgaag
     1321 tgagggtcgt cagggcgctg gccggaaccg gggtgccggt gcctccggtg gtcggctact
     1381 gcgaggacga ggcggtgacc ggagcgccct tttacgtgat ggagttcgtc gagggcccgg
     1441 tgatccgggg tcccgagcag gctgccgcct ttccctccga agaacagcgc cgccgcctcg
     1501 gcgagggcct ggtcgagacg atggcgaaga tccattcggt cgacgtgaac gaggttggcc
     1561 tttccgacct gggccgtcac gacgggtacg tccagcgtca gctcaagcgc tggtccggtc
     1621 agtgggagaa gtcgaagacc cgcgagttgc cgctggtcga agacgtccac tcccgcttgg
     1681 tcgaactggc gccgccgcag tcggcggtga ccctggttca cggcgactac cggcttgaca
     1741 acgtgatcta cagcccgacc ggcgaggtcg ccgccgtggt cgactgggaa ctctgcacga
     1801 tcggcgaacc gctggccgac ctcggtctgc tgatggtcta ctggcgcgaa gccggcgatg
     1861 aacagatccc gctgatcaca cccgcgaccg ttgaaccggg tttcccgaca cgggccgagc
     1921 tggccgatct ttacgcggag atcaccggcc gggacatctc gaaactggac tacttcgtgg
     1981 cgctcgggtt ctggaagctg gcgatcatcg ccgaaggcgt gctcgcccgt ttcgccgccg
     2041 gccagtacgg cgacgacatc ggcgacgtgg tcgagcgctc acaggccacg atcgatcttc
     2101 tggtcggcca ggccgacgag gcgatcaacc ggattgcata agtaagtcgg gcggggtccg
     2161 acccgcccga ggtcaggccg cctgaccgtt ggaggcggcc cgcttcttca ggaaccgtcc
     2221 gactacacca gggtgtccgg aggtcaggcc
//
LOCUS       DAOQMF010000299         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31219_length_2249_cov_4.332726, whole genome shotgun sequence.
ACCESSION   DAOQMF010000299 DAOQMF010000000
VERSION     DAOQMF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31219_length_2249_cov_4.332726"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLV77_05790"
     CDS             complement(<1..371)
                     /locus_tag="PLV77_05790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573314.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC15391.1"
                     /translation="MQIKPDEIASILRQRIEGIDPGSADLTEVGTVLSVADGIARIHG
                     LDNCMALEMLDLPHGVTGLALNLEADNVGAVLFGEWDKIVEGDTVKRTGRLLEIPVGE
                     EFLGRMVDPLGRPLDGKGDVKS"
     gene            complement(389..922)
                     /gene="atpH"
                     /locus_tag="PLV77_05795"
     CDS             complement(389..922)
                     /gene="atpH"
                     /locus_tag="PLV77_05795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNC15392.1"
                     /translation="MEELARVYAESLFGVAREKGNLDEVQAELAQFSDALEGNRELAV
                     FFFSPYFSSKEKLDGLNKAVEGTTPEFRNFLELLIEKHRMPAIFRIRRDFDDLWKKEN
                     DRIEVTVTSAVELDPAVSREVADAVEKQTGKQVDLTSRVDEGIIGGLVLQVGNMVLDS
                     SIRNNLEKLRQSVAQAA"
     gene            complement(922..1482)
                     /gene="atpF"
                     /locus_tag="PLV77_05800"
     CDS             complement(922..1482)
                     /gene="atpF"
                     /locus_tag="PLV77_05800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNC15393.1"
                     /translation="MLPLAAEAAEEEGGSFLVTPGLGLMIWTLVLFGFTFWVLKKFAF
                     PLIGEALDKRAATIRESIEAAEKQKEESDKLLADYRQRLTEAREQADEIVTKARKAAE
                     TTKSEASAEGQAKREELVDAARKDIEAEKTKALDDIRKEVANLTVLATERVTRKSLDD
                     AAHKELVEDALREVVFSALAGEERNN"
     gene            complement(1520..1789)
                     /gene="atpE"
                     /locus_tag="PLV77_05805"
     CDS             complement(1520..1789)
                     /gene="atpE"
                     /locus_tag="PLV77_05805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNC15394.1"
                     /translation="MDPSSIVPLAEVTGDVTDAGKAIALGVGAGLGSIGAGIGIGFIF
                     GKEIESVARQPELASELQSIRWLGFALTEAVAFYTFIFALIAFFL"
     gene            complement(1808..>2249)
                     /locus_tag="PLV77_05810"
     CDS             complement(1808..>2249)
                     /locus_tag="PLV77_05810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNC15395.1"
                     /translation="FALYAATANIAIPLVLTLIVWVSYHVEGIRAKGFIKYLASWLPS
                     GLEDMNPFGKAAIFLIEVVSHFVRLISLSVRLFANILAGHLLLLFMGGGLAVLLGIAA
                     LGVLTYPMAFAFYVFEVGIVATLQAFIFSILTAIYIGGATAESH"
BASE COUNT          398 a          689 c          731 g          431 t
ORIGIN      
        1 gacttgacgt cacccttgcc gtcgagcgga cggccgagcg ggtcgaccat gcggccgagg
       61 aattcctcgc cgaccgggat ctcgagcagg cggccggtgc gcttcaccgt gtcgccttcg
      121 acgatcttgt cccactcgcc gaagagcacg gcgccgacgt tgtcggcttc gaggttcagc
      181 gcgagtccgg ttaccccgtg gggcaggtcg agcatctcca gcgccatgca gttgtcgagc
      241 ccgtgaatac gggcgatacc gtcagcgacc gagagcacgg tgccgacctc ggtcaggtcg
      301 gcgctaccgg ggtcgatgcc ctcgatgcgc tgccgcagaa tgctggcaat ctcgtctggc
      361 ttgatctgca tctcggtcca aatcctcctt aggccgcctg agccacgctc tggcggagtt
      421 tctccaagtt gttgcgaatg ctgctgtcga gaaccatgtt cccgacctga agtacgagtc
      481 cgccgatgat cccttcatcg acgcggctgg tcagatcgac ctgcttgccg gtctgctttt
      541 caaccgcgtc ggcgacttcc cgggacacgg ccggatcgag ctcgaccgca ctggtcacgg
      601 tcacttcgat ccggtcgttt tccttcttcc agaggtcatc gaaatcgcgc cggatccgga
      661 aaatcgccgg catccggtgc ttttcgatga gcaactcgag gaagttgcgg aattcgggcg
      721 tggtcccctc gaccgccttg ttcaggccgt cgagcttctc cttcgaggag aagtagggac
      781 tgaagaagaa gaccgcgagc tcgcggtttc cttccagggc atcgctgaac tgtgcaagct
      841 cggcctgcac ttcgtccagg ttgcccttct ccctggctac gccaaagagg gactcggcat
      901 atacccgagc cagttcttcc atcagttgtt ccgttcctcg ccggccaggg cggagaagac
      961 gacttcgcgc agggcgtcct cgaccagttc cttgtgggcg gcatcgtcga gcgacttgcg
     1021 ggtgacccgc tcggtcgcga ggacggtcag gttggcgact tccttgcgaa tgtcgtccag
     1081 ggccttggtc ttctcggcct cgatgtcctt gcgggcggcg tcgaccagct cttcgcgctt
     1141 ggcctgccct tccgcacttg cttcggactt ggtcgtctcg gcggccttgc gggccttggt
     1201 gacgatttcg tcggcctgct cacgggcttc ggtgagtcgc tgacggtaat cggccagcag
     1261 cttgtccgac tcttccttct gcttttcggc agcctcgatg ctctcgcgga tcgttgccgc
     1321 gcgcttgtcg agggcttcgc cgatcagcgg gaaggcgaac ttcttcagca cccagaaggt
     1381 gaagccgaag agcaccaggg tccagatcat cagacccagc cccggcgtca ccaggaagct
     1441 gccgccctct tcctcggccg cttcggctgc cagaggcagc acggtcgcga tatgtgagag
     1501 aacaggctcc acggtcggcc tacaggaaga acgcgatcag cgcgaagatg aaggtgtaga
     1561 aggcgacggc ctcggtcaga gcgaagccga gccagcggat cgactggagc tcggaagcga
     1621 gctcgggctg acgggcaacc gactcgatct ccttgccgaa gatgaagccg atgccgatgc
     1681 cggcgccgat cgaaccgagt ccggcgccta cgccgagagc gatcgccttg ccggcgtcgg
     1741 tgacgtcacc ggtgacttcg gcgaggggca cgatggaact gggatccatt actgggactc
     1801 cttttggtta gtgactttcg gcggttgctc cgccgatgta gatagcggtc aggatggaaa
     1861 agatgaatgc ctgcagggtg gccacgatgc cgacctcgaa cacgtagaag gcgaaagcca
     1921 tcgggtaggt caggacgccg agagcggcga taccgagcag gactgccagg ccgccgccca
     1981 tgaagagcag aagcaggtgg ccggcgagga tgttggcgaa aagtcggacc gacagcgaga
     2041 tcaggcggac gaagtgggag accacttcga tcaggaagat cgccgccttg ccgaaggggt
     2101 tcatgtcctc caggccggag ggcagccagc tggccaggta cttgatgaag cccttggccc
     2161 ggatgccttc gacgtggtac gagacccaga cgatcagggt caggaccagc gggatcgcga
     2221 tgttcgcggt ggccgcatac agggcaaag
//
LOCUS       DAOQMF010000300         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31361_length_2244_cov_2.757880, whole genome shotgun sequence.
ACCESSION   DAOQMF010000300 DAOQMF010000000
VERSION     DAOQMF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31361_length_2244_cov_2.757880"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLV77_05815"
     CDS             complement(<1..514)
                     /locus_tag="PLV77_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNC15396.1"
                     /translation="MRAMVLESQPGDLVPRELPVPEPGPGQILIRVSTCGVCRTDLHI
                     VDGELSEPKLPLVPGHQIVGTVEAAGEDADRFGTGDRIGVPWLGWTDGDCRFCREGRE
                     NLCENARFTGYDSDGGYAEFAVADQRFCFPLSAAIDDTHVAPLLCAGMIGYRALAIAG
                     DGERLGLYGFG"
     gene            complement(533..>2244)
                     /gene="cofH"
                     /locus_tag="PLV77_05820"
     CDS             complement(533..>2244)
                     /gene="cofH"
                     /locus_tag="PLV77_05820"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HNC15397.1"
                     /translation="DIAAEASDRRWKESGTVVSLDSGIEAGAGEETANHDPGRPTIPL
                     PDWACPVSIGDLKHLIRETARLMPGVGVQVPPNLSDWWAELVEAGATDLGGLSANGDH
                     ISPEHPFPSPHQVRKELQPMGYALTERLCVYPQYMNADWMEQGVLDVIKLKYWSFIPR
                     LGSGRVSEETIDPGLAIEAIAKGREGEELTEDELTALFAETRPEVIEAMRVAADELRA
                     ELAGNTATFVVNRNINFTNICTVGCAFCGFGQGRRSPDAYESTEEEFIGRVEEAVEFG
                     ATELCMQGGIHPELDLEAYGSWLKLAKQVAPDIHLHAYSPMEINHMCEVSGLPPSEVF
                     EYLIECGLGSTPGTAAEVLDDGVRQRISPNKLPVARWVEIIEASHRAGLKSTSTVMFG
                     HIEEPRELARHMKVIRDLQGRTGGITEFVPLSFIPFNTLLGRTHGVEEISIDENLKHT
                     AAFRLALGKSVTNLQASWVKMGLPGATEALNWGVNDLGGTLMEESISRMAGSQHGVRL
                     EVEELVDAAHRAGRRAAQRNTSYEIIREFPDSVTSPPPEMLIDLAWPGEEPAPEIADP
                     AAA"
BASE COUNT          348 a          757 c          753 g          386 t
ORIGIN      
        1 atccgaaacc gtagagaccc agtcgctcgc cgtctccggc gatcgccagc gcccggtagc
       61 cgatcatccc ggcgcaaagg agcggtgcca cgtgagtgtc atcgatcgcc gcggaaaggg
      121 ggaaacagaa cctctggtcg gcgaccgcga actccgcgta cccgccgtcg gagtcataac
      181 cggtgaagcg ggcgttctca cagaggttct cccggccctc ccggcagaag cgacagtccc
      241 cgtcggtcca gcccagccag ggcacgccga tccggtcacc ggtcccgaag cggtccgcgt
      301 cttctcccgc ggcctcgacc gtgccgacga tctggtggcc gggaaccagc ggcagcttcg
      361 gctcgctcag ctcgccgtca acgatgtgca ggtcggtccg gcaaaccccg caggttgaaa
      421 cccggatcag aatctggccg ggaccgggct cggggacggg aagttcccgc gggacgaggt
      481 cgccgggctg gctttcgagg accatcgcgc gcatcgcccg gcccctcccg attcaggccg
      541 cggcgggatc cgcgatttcg ggggccggtt cctcccccgg ccaggccagg tcgatcagca
      601 tctcgggcgg cggggaggtc accgaatcgg ggaactcgcg gatgatttcg taagaggtat
      661 tccgctgggc ggcccggcga ccggcacgat gggcggcgtc gaccagttcc tcgacctcga
      721 gccggactcc gtgctgggaa ccggccatac gggagatgct ctcctccatc agggtgccgc
      781 cgagatcgtt cacaccccag ttgagcgctt cagtcgctcc cggcaggccc attttcaccc
      841 agctcgcctg gaggttcgtg accgacttgc cgagcgcgag gcggaaggcc gcggtgtgct
      901 tgaggttctc gtcgatcgag atctcctcca ccccgtgagt tcgcccgagc agcgtgttga
      961 acggaatgaa gctcagcggc acgaattcgg tgatgccgcc ggtgcggccc tgaaggtcac
     1021 ggatcacctt catgtgacgg gcaagttcgc gcggttcctc gatgtgaccg aacatcaccg
     1081 tcgaggtcga cttcagcccg gcccggtgtg aagcctcgat gatctcgacc cagcgggcca
     1141 ccggcagctt gttcggagaa atgcgctggc gcactccgtc gtcgagcacc tcggccgcgg
     1201 tgccgggggt cgaacccaga ccgcattcga tcaggtattc gaagacctca gacggcggca
     1261 gacccgagac ctcgcacatg tggttgatct ccatcggcga gtaggcgtgg aggtggatgt
     1321 cgggcgcaac ctgcttggcg agcttcagcc agctcccgta ggcctcgagg tccagttcgg
     1381 ggtggattcc gccctgcatg cagagctcgg tcgcgccgaa ctcgaccgcc tcctcgaccc
     1441 ggccgatgaa ctcctcctcg gtcgactcgt aggcgtcggg cgagcggcga ccctggccga
     1501 agccgcagaa ggcgcagccg accgtgcaga tgttggtgaa gttgatgttg cggttgacca
     1561 cgaaggtggc cgtgttgccg gccagttcgg cccgcagttc gtcggcggcg acccgcatcg
     1621 cctcgattac ttcgggccgg gtctcggcga agagcgcggt cagctcgtcc tcggtcagtt
     1681 cttccccctc gcggcccttg gcgatcgcct cgatcgccag cccggggtcg atcgtctcct
     1741 cgctgacccg gccggagccg agccgcggaa tgaagctcca gtacttgagc ttgatcacgt
     1801 cgaggacgcc ctgttccatc caatcggcgt tcatgtattg cgggtagacg caaagccgct
     1861 cggtcagggc gtaacccatc ggctgcagct ccttgcggac ctggtggggg ctcgggaaag
     1921 ggtgctcggg cgagatgtgg tcaccgttgg cggagagccc cccgaggtcg gttgccccgg
     1981 cctcgaccag ctccgcccac cagtcggaaa ggttcggtgg cacctggacc ccgacccccg
     2041 gcatcagccg ggccgtttcg cggatcaggt gcttcaggtc cccgatcgat accgggcagg
     2101 cccagtccgg cagcgggatg gtcgggcgtc cggggtcgtg gtttgccgtt tcttcgccgg
     2161 ccccggcttc gatgccggaa tcgaggctca cgaccgtccc cgattccttc cagcgccggt
     2221 cggaagcttc ggccgcgatg tcag
//
LOCUS       DAOQMF010000301         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31451_length_2240_cov_3.269108, whole genome shotgun sequence.
ACCESSION   DAOQMF010000301 DAOQMF010000000
VERSION     DAOQMF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31451_length_2240_cov_3.269108"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1764
                     /locus_tag="PLV77_05825"
     CDS             328..1764
                     /locus_tag="PLV77_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC15398.1"
                     /translation="MCAGVLMIVLDATVVNVALPAIQDDLGFSQSSLAWVVNAYLIAF
                     GGLLLLSGRLGDLISRRDVFLVGLVVFTTASLLCGFAQSDVMLVAFRFLAGIGGALTA
                     AVVLGMIVTMFPEPSEQAKAIGVYAFVASAGGSVGLLAGGILTQALSWHWIFFINIPI
                     GIATGYFSLKLLERDKGLGFSHGADILGAVLITASLMLGVFTIVEPAAVHGWGDTTTL
                     LCSAGSFLLLIAFLVRESTVANPLVPLRIFKSRNVSGANAVQALTVAGMFGLFFLGVL
                     YLQEVLGYDALQTGIAFLPTTLVMGTLSVRFSEPLIMRFGARNCLIVGLVLIAIGLAL
                     FARVPVDGNYWTDVLPSTLLLGSGVGVCFPSLMTLAMSGATRADAGLASGLVNTTAQV
                     GAAIGLAVLATIAATKTDNLLDAGEPRPEALVAGYEVAFWVAAGLVVVALIVAFIVLE
                     PARPQEAGAEGPDGMTEAELESEFGTTL"
     gene            complement(1775..2191)
                     /locus_tag="PLV77_05830"
     CDS             complement(1775..2191)
                     /locus_tag="PLV77_05830"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC15399.1"
                     /translation="MTVRLNPYLNFREEAREAMTFYQSVLGGELTINSFADFHASEDP
                     AEQEKVMHAHLVTADGFNLMGADTPNSMEFNGQSGVSVSLSGDDEETLRRWFEGLSEG
                     GTVVMPFEQAPWGATFGMVVDRFGTSWLVNAGPESQ"
BASE COUNT          325 a          800 c          669 g          446 t
ORIGIN      
        1 gccgctgatc atgctcacct cgcgccgcga cgtaatgggc gagggcttcg aaaaccaccg
       61 gttgaccacg atcgtcgcct cgatcttcgc cgcgatcatc atcgcgctca acctcttcct
      121 gctcttccag accttcaccg gaaactgagg ccaaccggac cggaccggac cgtccgcggc
      181 ggcccgggcc gaacggccac ttttgttagt ctcagccgcc tgaccgacca gtctgaaaac
      241 acggtaattc catccgattc agactccgat aaccagcccg accatccgaa ggatccggat
      301 cgttcccgct ggattgcgct ctacgtcctg tgcgccgggg tattgatgat cgttctcgac
      361 gcaaccgtcg tgaacgtcgc cctgccggca atccaggacg atctcgggtt ttcccaatcc
      421 agcctggcct gggtggtcaa cgcgtacctg atcgccttcg gcgggctgtt gctcctctcg
      481 gggaggctgg gcgacctgat ctcccggcgc gacgtgttcc tcgtcggcct ggtcgttttc
      541 accaccgcgt ccctactttg cggtttcgcc cagagtgatg tgatgctggt cgccttccgt
      601 ttcctggccg ggatcggggg ggccctgacc gccgcggtcg tgctgggcat gatcgtgacc
      661 atgttccccg agccgagcga acaggcgaag gcgatcggcg tctatgcctt cgtcgcctcg
      721 gccggcggtt cggtcggcct cctggccggc ggcatcctca cccaggcgct ctcctggcac
      781 tggatcttct tcatcaacat cccgatcggc atcgctaccg gctacttctc actgaagttg
      841 ctcgagcgcg acaagggact cggcttctcc cacggagccg acatcctcgg cgccgtgctg
      901 atcaccgcct cgctgatgct cggtgtcttc acgatcgtcg aaccggccgc cgtgcacggt
      961 tggggagaca cgacgaccct gctctgctcg gccggttcct tcctgctcct gatcgccttc
     1021 ctcgtgcggg aatcaacggt cgccaatccg ctggtcccgc tgcggatctt caagtcccgc
     1081 aacgtctccg gggccaacgc cgtccaggcc ctgaccgtgg ccggcatgtt cggactcttc
     1141 ttcctcgggg tgctctacct gcaggaagtc ctcggctacg acgcgctcca gaccggcatc
     1201 gccttccttc ccaccaccct ggtcatgggc acgctttcgg tcaggttctc ggagccgctg
     1261 atcatgcgtt tcggcgccag aaactgcctg atcgtcgggc tggtgctgat cgcgatcggc
     1321 ctggccctct tcgcccgggt cccggttgac ggcaactact ggaccgacgt gctgccctcc
     1381 accctcctgc tcggctccgg ggtcggggtc tgcttcccct cactcatgac cctggccatg
     1441 tccggtgcga ccagggccga cgccggtctc gcctccgggc ttgtcaatac gaccgcccag
     1501 gtcggtgccg cgatcgggct ggccgtgctc gcgaccatcg ccgcaaccaa gaccgacaac
     1561 ctcctcgacg ccggcgaacc ccggcccgag gccctggtcg ccggctacga ggttgccttc
     1621 tgggtcgccg ccgggctggt cgtggtcgcg ctgatcgtcg ccttcatcgt gctcgaaccg
     1681 gcccgcccgc aggaggccgg ggcggaaggc ccggacggca tgaccgaggc cgaactcgaa
     1741 tccgaattcg gcaccaccct ctagccggcg agcgctactg cgactcggga ccggcgttga
     1801 ccagccagct ggtgccgaag cggtcgacca ccatgccgaa ggtcgcgccc cagggtgcct
     1861 gctcgaacgg catcacgacc gttccgcctt cgctgagccc ctcgaaccat cggcggagcg
     1921 tctcttcgtc gtcaccgctg agcgaaacgg acactcccga ctgcccgttg aattccatcg
     1981 agttgggggt gtcggcgccc atcaggttga acccgtctgc ggtgaccagg tgcgcgtgca
     2041 tcaccttttc ctgctcggcc ggatcctcgc tggcgtggaa gtcggcaaac gagttgatcg
     2101 tgagctcgcc gcccagcacc gactggtaga aggtcatcgc ctcacgggct tcctcgcgga
     2161 agttcaggta cggattcagt cgaacggtca tgccggtcct ctcctcggtt gggatccgga
     2221 ccgatactac tcaggggatt
//
LOCUS       DAOQMF010000302         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31588_length_2234_cov_3.747132, whole genome shotgun sequence.
ACCESSION   DAOQMF010000302 DAOQMF010000000
VERSION     DAOQMF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31588_length_2234_cov_3.747132"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLV77_05835"
     CDS             <1..452
                     /locus_tag="PLV77_05835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC15400.1"
                     /translation="PSYARRVLALGHEIGNHSYDHALLPVSSDIRHTNRSIIQATHFR
                     PCLFRPPYGAVSSSLVASARAARMKVVDWDVDTTDWKLPGAGYIKSNVLGNAHSGSIV
                     LMHDGGGPRSQTVEALPGIIDGLRGRGYRLVTVSQLLGNRMIYRPVP"
     gene            complement(513..>2234)
                     /locus_tag="PLV77_05840"
     CDS             complement(513..>2234)
                     /locus_tag="PLV77_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15401.1"
                     /translation="ADKNQKAVVINADTGERHPIWVNIDANAGSDQDRVLEIKPAVNF
                     DEKGHYIVALRNLVTAAGKPIAAPTAFRYYRDQIRSSQKPINDRRAHFEQMFETLKQA
                     GIKRRDLYLAWDFTTASNENNYKRALSMRNRAFAELGDTNLGDQIVQGSAPQFTIDSV
                     QVLPTGDIARKIKGHYTVPCFLEPSCGPGGTMNLDANGLPSRNGNYTANMECIIPRVA
                     VQPGAEKTRPMVFGHGLFGDASGVNGGPNPPLAQKGMTICGTDEIGMSNSDVVQVMTS
                     ALQNLSHFNVLADRLAQGLINELFLARLMYHPNGLGTAPAFQDGDGFTAGQSLIKTDH
                     VYYMGASQGGIMGGPLTALSPDFTQSSLLVGSMNYSVLLPRSIDFDLYSAILYPSYPG
                     DLAHPLLFQLMQMLWDRSEPNGYAHVMTDNPPPDTPEHKVTLMIAVGDHQVSNYASEV
                     MARTLGMSTNQTPVDEGRWPDYDILWNVPRLSASDYPFHGNNVIYFDGGPPRPEPGNP
                     SKTIGTDVPPFANVPNRSAQDPHGAPGGASVAVEMTSTFLQPNGYISDVCSPHACYGD
                     AWDGSLP"
BASE COUNT          359 a          671 c          786 g          418 t
ORIGIN      
        1 atccgtcata cgcccgacgg gttctggcgc tgggccacga gatcggcaat cacagctatg
       61 accacgccct gcttccggtc agctccgaca tccgtcacac caaccggagc atcatccagg
      121 cgacccactt ccggccctgc ctgttccgac cgccctacgg cgcggtcagc agctcgctcg
      181 tggcctcggc ccgggcggcc cggatgaagg ttgtcgactg ggatgtcgac acaaccgact
      241 ggaagctgcc cggtgccggg tacatcaagt cgaacgtcct cggtaacgcg cattcagggt
      301 cgatcgtcct gatgcatgat ggcggcggcc cccgcagcca gacggtggag gcgctgcccg
      361 gaatcatcga cgggctccgc gggcggggtt accggctggt cacggtctcg cagctcctcg
      421 gcaaccggat gatctaccgc ccggtcccct gaaaagcgaa agccccgggc gtttccgcac
      481 cggggctttc tctcggaatc cgtttctgcc ggttacggca ggctgccgtc ccaggcgtcg
      541 ccgtagcagg cgtggggtga gcagacgtca ctgatgtagc cgttcggctg caggaaggtc
      601 gaggtcattt cgaccgcgac gctggccccg ccgggagctc cgtgcggatc ctgggccgaa
      661 cggttcggaa cgttggcgaa ggggggcacg tcggtgccga tcgtcttcga cggattgccc
      721 ggctccggtc gcggcggtcc accatcgaag tagatgacgt tgttgccgtg gaacggatag
      781 tcggacgcgg acagcctcgg gacgttccag aggatgtcgt aatcgggcca gcgtccttca
      841 tcgaccggcg tctggttggt gctcatgccg agggtccggg ccatcacttc cgaggcgtag
      901 ttggagacct gatggtcgcc gaccgcgatc atcagggtga ccttgtgctc gggggtgtcc
      961 ggcggcgggt tgtcggtcat cacgtgggcg taaccgttgg gctcgctgcg gtcccagagc
     1021 atctgcatca gctggaacag gagcggatgg gccagatcgc ccgggtagga cgggtagagg
     1081 atcgccgagt agaggtcaaa gtcgattgaa cgcggcagca gcaccgagta gttcatcgaa
     1141 ccgaccagca gcgaggactg ggtgaagtcg ggcgagagcg cggtcagcgg accgcccatg
     1201 atcccgccct ggctggcgcc catgtagtag acgtgatcgg tcttgatcag cgactgtccg
     1261 gccgtgaaac cgtcaccgtc ctggaaggcc ggagcggtgc cgaggccatt cgggtggtac
     1321 atcagacggg ccaggaacag ctcgttgatc aggccctggg ccagccggtc ggcgagcacg
     1381 ttgaagtgcg acaggttctg cagggcgctg gtcatcacct ggaccacatc cgagttggac
     1441 atgccgatct cgtcggtgcc gcagatcgtc atgcccttct gggccagcgg cgggttgggg
     1501 ccgccgttga cgccggaggc atcaccgaac aggccgtggc cgaagaccat cggccgggtc
     1561 ttttcggcac cgggctggac cgcgacccgg ggaatgatgc attccatgtt cgccgtgtag
     1621 ttgccattgc gcgagggcag gccgttggcg tcgagattca tggttccgcc gggaccgcag
     1681 gacggttcaa ggaagcaggg aaccgtgtag tggcccttga tcttgcgggc gatgtccccg
     1741 gtgggaagca cctggacgct gtcgatcgtg aactgcggag ccgagccctg gacgatctgg
     1801 tcgccgaggt tcgtgtcacc gagttcggcg aaggcacggt tgcgcatgct cagcgcccgc
     1861 ttgtagttgt tctcgttgct ggcggtggtg aagtcccagg caaggtagag gtcccggcgc
     1921 ttgattccgg cctgcttcaa ggtctcgaac atctgctcga aatgagcccg gcgatcattg
     1981 atcggcttct ggctggagcg gatctgatcc cggtagtagc ggaaggcggt cggcgccgcg
     2041 atcggcttgc cggcggcagt gacgaggttg cgcagggcga cgatgtagtg gcccttctcg
     2101 tcgaagttga cggcgggctt gatctccagc acccggtcct ggtcggaacc ggcgttggcg
     2161 tcgatgttga cccagatcgg atggcgctcg ccggtgtcgg cgttgatcac gaccgccttc
     2221 tggttcttgt cggc
//
LOCUS       DAOQMF010000303         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31670_length_2231_cov_3.230239, whole genome shotgun sequence.
ACCESSION   DAOQMF010000303 DAOQMF010000000
VERSION     DAOQMF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31670_length_2231_cov_3.230239"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2231
                     /locus_tag="PLV77_05845"
     CDS             <1..>2231
                     /locus_tag="PLV77_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNC15402.1"
                     /translation="MVRSYPSRGTGYASQVTPPPHLTGLRIDALDSFGREEAHPPVVV
                     ASAIALAESVPDASLRPAGFRLTVGEEHDPSQVAADLVAAGYERVDQVQERGQFAVRG
                     GILDVFSATEDRATRVDFFGDEIESMRWFSTFTQRSLGQAESIELSPAAELDAEHREM
                     AELIIAEARENETDLDLTEALPLDHFRAPLDLIPDSTAVILTADEEIEPALKDHWEDV
                     TTAMHADDARHLYEDVAGPLNQRASLILVRPGEGEGALRAMSAETPARNLKEAEAELR
                     KLVEAGYRTIVAFDSRGEAERARYGFDRLDTKIVDQGPVSIEPGVSLVESRLAEGFVS
                     PRTKVAVLPFRRLLNRRRRADERAPEAVRGRLTFSELRVGDFVVHEDHGVARFAGFET
                     REVGGITRDYLYLEYKGEDRVYVPTDQFAKLSRYSGGAGTPTLSALGGRKWLNMKARA
                     RGAADEIAGELVNLYAERKTRKGHAFSPDSEWQMELEANFPWRETADQIEAIEAVKAD
                     MESEQPMDRLVCGDVGYGKTEVAIRAAVKAASDGKQVMILAPTTILAQQHLGTFRERL
                     ADLPFRVDGVSRLRKPAEVKQVVAEWGEGKVDVLIGTHRLLSRDVRAKDLGLIVVDEE
                     QRFGVKQKELLRQMKLKVDVLSMSATPIPRTLQMSMSGLRDISVIETPPEGRRPVRTY
                     VGPWDEQLVERAIKREISRKGQVFFLHNRVESLDEAAESLRALVPGVRIQVAHGQMED
                     QEL"
BASE COUNT          380 a          776 c          749 g          326 t
ORIGIN      
        1 atggttcgct cctacccttc ccgcggcacc ggctatgcct cgcaggtaac cccgccgccg
       61 catctgaccg gcctcaggat cgacgccctc gattccttcg gccgcgagga ggctcacccc
      121 ccggtcgtgg tcgcctccgc gatcgcgctc gccgaatcgg tgcccgacgc ttcgcttcgg
      181 ccggccggct tccggctcac ggtcggcgag gaacatgatc cctcgcaagt cgcggccgac
      241 ctggtcgcgg ccggctacga acgggtggac caggtccagg aacgcggcca gttcgcggtc
      301 cggggcggca tcctcgacgt cttctcggcg accgaggacc gggccacccg ggtcgatttc
      361 ttcggcgacg agatcgaatc gatgcgctgg ttttccacct tcacccagcg atcgctcggg
      421 caggcggaat caatcgagct ctccccggcg gccgaactgg acgccgaaca ccgcgagatg
      481 gccgaactga tcatcgccga ggcccgtgag aacgagaccg acctcgacct gaccgaggcc
      541 ctgccgctcg accactttcg cgccccgctg gacctgatcc ccgattcgac cgcggtgatc
      601 ctgaccgccg acgaggaaat cgagccggcc ctgaaggacc attgggagga cgtcaccacg
      661 gcgatgcatg ccgatgacgc ccggcacctc tacgaggacg tggccgggcc gctgaaccag
      721 cgggcgtcgc tgattctcgt ccgcccgggc gagggcgaag gggcgctccg ggcgatgagc
      781 gccgagacgc cggcccgcaa cctcaaggaa gccgaagcgg aactgcggaa gctggtcgag
      841 gccgggtacc ggacgatcgt cgccttcgac tcccgcggcg aggctgaaag ggcccgttac
      901 ggctttgacc ggctcgacac gaagatcgtc gaccaggggc cggtttcgat cgagcccggg
      961 gtcagtctgg tcgaatcacg gctggcggag ggcttcgttt ccccccggac caaggtcgcg
     1021 gtactgccct tccgccggct gctcaaccgg cgccgccggg ctgacgaaag ggctccggaa
     1081 gccgttcgcg gccggctcac cttctccgaa ctgagggtcg gcgacttcgt ggttcatgaa
     1141 gaccacgggg tcgcccgctt cgccggattc gaaacccgcg aagtgggcgg catcacccgc
     1201 gactacctct acctcgaata caagggcgag gaccgggtct atgtgcccac cgaccagttc
     1261 gccaagctct cgcgttattc cggcggggca ggcaccccca ccctttccgc cctcgggggc
     1321 aggaagtggc tgaacatgaa ggcccgcgcc cgcggcgccg ccgacgagat cgccggcgag
     1381 ctggtcaacc tctacgccga gcggaagacc cgcaagggcc acgccttcag ccctgactcg
     1441 gaatggcaga tggagcttga agcgaacttc ccgtggcgcg agacggccga ccagatcgag
     1501 gcgatcgagg cggtcaaggc cgacatggaa tccgagcagc cgatggaccg gctggtctgc
     1561 ggcgacgtcg gctacggcaa gaccgaggtg gcgatccggg ccgcggtcaa ggccgcctcg
     1621 gacggcaagc aggtgatgat cctggccccg accacgatcc tcgcccagca gcatctcggc
     1681 accttccgcg aacgcctcgc cgacctgccc ttccgggtcg acggggtcag ccgcctgcgc
     1741 aagcccgccg aggtcaaaca ggtggtcgcc gaatggggcg agggcaaggt tgacgtcctg
     1801 atcggcaccc accggctgct ctcccgggac gtccgggcga aggacctcgg tctgatcgtg
     1861 gttgacgagg aacagcgctt cggggtcaag cagaaggaac tgctgcgcca gatgaagctc
     1921 aaggtggacg tgctttcgat gtcggcgacc ccgatcccgc ggaccctgca gatgtcgatg
     1981 tccgggctga gggacatctc ggtgatcgag accccgcccg agggtcgccg cccggtccgc
     2041 acctacgtcg gcccctggga cgaacagctg gtggaacggg cgatcaagcg ggagatcagc
     2101 cgcaagggcc aggtcttctt cctccacaac cgcgtcgaat cactcgacga ggccgccgag
     2161 agcctccggg cgctggtgcc cggggtccgg atccaggtcg cccacggcca gatggaggat
     2221 caggagctcg a
//
LOCUS       DAOQMF010000304         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31695_length_2230_cov_3.702529, whole genome shotgun sequence.
ACCESSION   DAOQMF010000304 DAOQMF010000000
VERSION     DAOQMF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31695_length_2230_cov_3.702529"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..931)
                     /locus_tag="PLV77_05850"
     CDS             complement(68..931)
                     /locus_tag="PLV77_05850"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC15403.1"
                     /translation="MLGASGFFGSRIAAELAAAGIEVLGPERSESELLDPAGLSELVA
                     ATAPAAVVNAAGMTSPASARANPSACFAVNAGGALNLLEALRQRAPGAQLVSLSSAAV
                     YAGSAPFDESSATAATTPYAASKLAMETICGQYRRGYGIPVTVLRCFNLIGPGEPSTQ
                     ASSEFVRAALAAGTGGRAEVEVGEPATARDFTDVRDAARAVRRVLEEEATGTLNLCSG
                     KATSLAELAALTGELTGVDLTLRGSGTGRPASGLLSIRGDFSKLRELTGWRPGITLEH
                     SLTDLIESLRP"
     gene            complement(967..1914)
                     /locus_tag="PLV77_05855"
     CDS             complement(967..1914)
                     /locus_tag="PLV77_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC15404.1"
                     /translation="MTVSVVTGGAGFVGSHLCDALLEKGNKVICIDNLDTGSLQNIEH
                     ITNPDFTFLPQDITEEVHLDGEVDFVYHLASPASPIDYARLPLHTLKVGAQGTWHMLG
                     VAKFKRARFLLASTSEVYGDPQVHPQEESYWGHVNPIGPRGVYDEAKRYAEALTMAYH
                     RQQGVDTSIVRIFNTYGSRMRAHDGRAIPTFMRQALQNQPLTVFGDGSQTRSFCFVDD
                     LVRGLIALAESGEHLPVNIGNPDEYTLLQLANAVIEASGAESTITFEPLPTDDPQVRQ
                     PNIDRAKELLGWEPTVPLSEGLKKTIEQSGRDRLIGKTG"
     gene            2001..>2230
                     /locus_tag="PLV77_05860"
     CDS             2001..>2230
                     /locus_tag="PLV77_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15405.1"
                     /translation="MSAVRRKAIGVLLLAGLVLFFLPAASGAVPAWQSAPVIPSLSNG
                     DQARLKDRVAAGERTGLRSGVFAKVGDSNTEFS"
BASE COUNT          333 a          709 c          790 g          398 t
ORIGIN      
        1 gtctggaact tctcgtagtt ggtggtgtcg acggtttcgg tgctgttcgc tgccatggcg
       61 ggtgatccta tgggcggagg ctctcgatga ggtccgtgag gctgtgctca agcgtgatcc
      121 cggggcgcca gccggtcagc tcgcgaagct tcgagaagtc cccgcggatc gaaagcagcc
      181 cggacgcggg ccgtccggtt ccggagccgc gcagggtcag gtcaaccccg gtcagctcgc
      241 cggtcagggc ggccagttcc gccaggctcg tcgcctttcc cgagcagagg ttgagggtgc
      301 cggttgcttc ttcctcgaga acccggcgga ccgcccgggc ggcgtcgcgc acgtcggtga
      361 agtccctggc ggtggccggc tccccgacct ccacctcggc ccgtccgcca gtcccggcgg
      421 ccagggcggc ccggacgaac tctgaactcg cctgggttga aggctcgccg gggccgatca
      481 ggttgaagca gcgcagcacc gtcaccggta tcccgtagcc gcgtcggtac tggccgcaga
      541 tcgtctccat ggccagcttc gaagcggcgt agggcgtggt cgccgcggtc gctgaagatt
      601 cgtcgaaggg cgctgacccg gcgtagaccg cggccgagga aagggacacg agctgcgccc
      661 cgggggcccg ttgccgcagc gcttcaagca ggttcagcgc cccgcccgca ttgaccgcga
      721 agcaggcgga agggtttgcc cgggccgatg ccggtgaggt catccccgcg gcgttgacca
      781 cggccgccgg tgcggtggca gcgaccagtt cgctgaggcc ggcgggatcg agcagctcgg
      841 attcgctccg ctccggaccg agcacctcga ttccggccgc ggcgagttcg gccgcaatcc
      901 ggctcccgaa aaagccggaa gccccgagca cgagaactct cccgggcccg gtcacggtcc
      961 tggaattcag ccggtcttgc cgatcagccg gtcgcggccc gactgttcga tcgtcttctt
     1021 gaggccctcg gacaggggca cggtcggctc ccagccgagc agctccttcg cccggtcgat
     1081 gttgggctgg cggacctgcg ggtcatcggt cggaagcggc tcgaaggtga tcgtcgattc
     1141 ggccccggaa gcctcgatca ccgcgttcgc gagctgcagc agcgtgtact cgtcggggtt
     1201 gccgatgttg accggcaggt gctcgcccga ttcggccagc gcgatcaggc cgcggaccag
     1261 gtcatcgacg aaacagaagg accgggtctg cgaaccatcg ccgaaaacgg tcagcggctg
     1321 gttctgcagg gcctgccgca tgaaggtcgg gatcgcccgc ccgtcatggg cgcgcatccg
     1381 tgagccgtag gtgttgaaga tccgcacgat cgaggtgtcg acgccctgct ggcggtggta
     1441 ggccatggtc agggcttcgg cgtaccgctt cgcttcgtcg tagaccccgc gcgggccgat
     1501 cgggttgacg tggccccagt agctctcttc ctgggggtgt acctgggggt cgccgtaaac
     1561 ctcggaggtc gaggcgagca ggaagcgggc ccgcttgaac ttggccacgc cgagcatgtg
     1621 ccaggtgccc tgggcgccga ccttcagggt gtgaagcggc agccgcgcat aatcgatcgg
     1681 gctggccggg gaggccaggt ggtagacgaa gtcaacctcg ccgtcgaggt ggacttcctc
     1741 ggtgatgtcc tgcggcagga aggtgaaatc gggattcgtg atgtgctcga tgttctggag
     1801 tgatcctgtg tccaggttgt cgatgcagat caccttgttg cccttttcga gcagggcatc
     1861 gcaaagatgg gatccgacaa aaccggctcc gccggtaact acgctgactg tcatgggcgg
     1921 atcgtattca aggttcggcc gacgatgccg tcctgatcgt ctaccgttta ctgcctgtca
     1981 cttcgggggg attggaagcc atgagcgccg tcaggcgaaa agcaatcggg gtcctgctgc
     2041 ttgccggcct tgtcctcttc ttcctgccag ccgcctcggg agcggtcccg gcctggcagt
     2101 cggccccggt tatcccctct ctttcgaacg gggatcaggc caggctgaag gaccgggtcg
     2161 cggccggcga gcggaccggg ttgcgcagcg gcgtcttcgc caaggtcggg gattcgaaca
     2221 ccgagttctc
//
LOCUS       DAOQMF010000305         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_31744_length_2228_cov_3.209848, whole genome shotgun sequence.
ACCESSION   DAOQMF010000305 DAOQMF010000000
VERSION     DAOQMF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31744_length_2228_cov_3.209848"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLV77_05865"
     CDS             <1..347
                     /locus_tag="PLV77_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15406.1"
                     /translation="VASGAAEQVAVKDEDLHVRLFSTLMSTESVSNEQIYRLRAAASG
                     REAIAPVKDGVVYRDAETGEELEPVAAVLPNTGEGSSLPRTPVNLRVCRRCEQLTPRD
                     LDTCEFCGLPAS"
     gene            complement(424..1272)
                     /locus_tag="PLV77_05870"
     CDS             complement(424..1272)
                     /locus_tag="PLV77_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15407.1"
                     /translation="MAEPRSHFVSRPALRLGALVAATLGLFACLVLAGAFGDADGKSN
                     RRVVLGATRDKVTPNCGTRSGARACIAEGKVTGFQVFQKGVTGKNFVVPFKKGKVVAW
                     SIQLSNPLKEDSRRFGPAQQPYFNKLFGSPSKARISILRPIKKGARYPPRYKLIRASG
                     TQKLNRYFGSEIKFAIKPLKVIRGDVVALTIPTWAPAFWVPMACSPSDRGTANAARCA
                     DARKANTWRGSRGPGQCAIGTNAFDQPNEALRNSHPQQKVNSIKRYACYYDAGRLLYK
                     ATVVAP"
     gene            complement(1389..>2228)
                     /locus_tag="PLV77_05875"
     CDS             complement(1389..>2228)
                     /locus_tag="PLV77_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15408.1"
                     /translation="MLRSPLTTDGLRVARVFAVLSGLAALILIAFAPHAEAKSTQRIE
                     LGARNINTLQPNCGRDFTRDCTVEGKVTAFQSLSARYPGRNFVVPFNGKLVAWSISLS
                     NPTRVEIDNNGAQLPSFNLIFGSPAQANIAILRQVEKRKKGGPRFKLVRKSPIQILNP
                     YFGTKVTFALDVPLNVYEGNVVALTIPTWAPALWKPKACNQTDFGILDPGRCDQAEKA
                     YTWRGSRVARGEPETCGLSNDGGPNEQLQKTAPQTHLDSVRRYGCYYGGNVLLYSAII
                     VGR"
BASE COUNT          403 a          671 c          744 g          410 t
ORIGIN      
        1 atgttgcttc cggtgccgcg gagcaggtag ccgtgaaaga tgaggatctt catgtccggc
       61 tattctccac cttgatgagc accgaaagcg tctccaacga acagatctac cgacttcgtg
      121 ccgcggcttc cggtcgcgag gccatcgccc ccgtcaagga cggcgtcgtc taccgggacg
      181 ccgaaaccgg cgaggaactc gaacccgtcg ccgccgtgct gccgaatacc ggagagggct
      241 ccagcctccc ccgcaccccg gtgaaccttc gcgtctgccg ccgctgcgag cagctaaccc
      301 cccgcgatct cgacacctgc gagttctgcg gcctccccgc ttcctgattt cacgagtttc
      361 atccgggtgc ttgtacttag ttggccactg accaactaag tacaagcacc cggatcctca
      421 gtcttaggga gcgacgacag tcgccttgta gagcaggcgg ccggcgtcgt aatagcaggc
      481 gtatcgcttg atcgaattga ccttctgctg gggatgcgaa ttcctcaggg cttcattcgg
      541 ctgatcgaag gcattggtgc caatcgcgca ttggccgggt ccgcggctgc cccgccaggt
      601 gttggccttg cgcgcgtcgg cgcagcgggc cgcattcgca gtgccccgat cggaaggact
      661 gcatgccatc ggaacccaga aggccggcgc ccaggtcggg atcgtgaggg cgacgacgtc
      721 accgcggatc accttgagcg gcttgatcgc gaacttgatc tcgctgccga agtagcgatt
      781 cagcttctgg gtgccgctgg ccctgatcaa cttgtagcgg ggcgggtacc tggcgccctt
      841 tttgatcggc cggaggatcg agatccgtgc cttcgagggt gagccaaaga gcttgttgaa
      901 gtaaggctgc tgggcaggcc cgaaccggcg gctgtcttcc ttgagcggat tcgaaagctg
      961 gatcgaccag gccacgactt tgcccttctt gaagggaacc acgaagttct tgccggtgac
     1021 ccccttctgg aagacctgga aaccggtcac cttgccttcc gcgatgcagg cccgtgcgcc
     1081 ggacctggta ccgcagttcg gggtgacctt gtccctggtt gccccgagca cgacccgccg
     1141 gttgctcttg ccatcggcat cgccgaaagc gccggcaagg acgaggcagg cgaagagacc
     1201 cagagtggct gcaacgagcg cgccgaggcg aagggccggg cggctcacga aatgtgaacg
     1261 aggttcggcc atacaggatc caacaccgag gctacgccac aggtgcggaa cttcaagtca
     1321 gagcggcggt cgggaaatcc tgcccggacg gtccaacggc tcgtagccgg ggaaatatcc
     1381 gaccggggtc agcggccgac gatgatcgcc gagtagagca ggacgttgcc gccgtagtag
     1441 cagccgtaac ggcgaaccga gtcgagatgg gtctgcggag cggtcttctg gagctgctcg
     1501 ttcgggccgc cgtcgttgct cagtccgcag gtttcgggtt caccccgggc cacgcgactg
     1561 ccacgccagg tgtaggcctt ctcggcctga tcgcaacgac cggggtcgag aatgccgaaa
     1621 tcggtctggt tgcaggcctt cggcttccag agcgccggcg cccaggtcgg gatcgtgagt
     1681 gcgacgacgt tgccttcgta gacgttgagc ggaacgtcga gcgcgaaggt gaccttggtc
     1741 ccgaagtagg gattgaggat ctggatcggg ctcttgcgaa ccagcttgaa gcgcgggcca
     1801 cccttcttgc gcttctcgac ctgcctgagg attgcgatat tggcctgtgc cggtgaaccg
     1861 aagatcaggt tgaaggaggg aagctgggct ccgttgttgt cgatctcgac ccgggtcggg
     1921 ttggaaagtg aaatcgacca ggcgaccagc ttgccgttga acggcaccac gaagttgcgc
     1981 cccggatacc gggcggaaag tgactggaag gcagtcacct tgccttcgac cgtgcagtcg
     2041 cgagtgaagt cccggccgca gttcggctga agggtgttga tgttgcgggc gccgagttcg
     2101 atccgctgcg tggacttggc ctccgcatgc ggtgcgaagg cgatcaggat cagggcggca
     2161 aggccgctca ggaccgcgaa aacccgggcg acccgaaggc cgtcagttgt gaggggggag
     2221 cgaagcat
//
LOCUS       DAOQMF010000306         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_32098_length_2212_cov_3.638850, whole genome shotgun sequence.
ACCESSION   DAOQMF010000306 DAOQMF010000000
VERSION     DAOQMF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32098_length_2212_cov_3.638850"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLV77_05880"
     CDS             <1..789
                     /locus_tag="PLV77_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15409.1"
                     /translation="HMTTRADNGEELITPKQIARGRGDDYLIRINTQAARRNLRIYLR
                     PLGEPNRCLNYYAGVDCAGNVRGGDYSYGWYKQAFRRIAIITRGGAKRGFINARLKDL
                     RLPKVQNVGQSRRLPRRLPKAPVSLVWSPLPAGSPTVRANLPARYWPGKDWVDWVGTD
                     FYNRYNNWKHLGRFYKKWALGKNKPMALTEFGLWGSDSPSFIKRIFKFTRNRKKIRMM
                     VYYQDFGESNSFRIQNFPQGRKVLSQFLNKRDYPKFAPKYPRFR"
     gene            complement(856..2013)
                     /locus_tag="PLV77_05885"
     CDS             complement(856..2013)
                     /locus_tag="PLV77_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC15410.1"
                     /translation="MNPRDYLEIDRLLTDEERLIKETVSRYVNEKYLPGVAEHFENGT
                     FDTAVPKALGDLNVLGMHLKGYGCAGTSAVAYGVACLELEAGDSALRSFCSVQGSLAM
                     FAIWKWGSEEQKQRWLPGMAAGDLIGCFGLTEPDSGSDPGSMRTTAKKDGEDWILNGS
                     KMWITSGSIADVAVVWAKTDDGVQGFLVEPGMKGFTAPEMHHKLSLRASVTSELAFDD
                     VRIPDANRLPEVQSLKGPLSCLNEARYGIVWGVVGAARSCFEAALDYAMTRKQFDEPI
                     ASFQLTQAKLADMATKVQQGYLLALRLGQLKDEGRIEPEHISMGKRANARMALDVART
                     ARGILGANGITLEYPVLRHANNLESVFTYEGTDEIHTLSIGMALTGIPAFR"
BASE COUNT          385 a          750 c          693 g          384 t
ORIGIN      
        1 cacatgacca cccgcgccga caacggcgag gagctgatca cgccgaagca gatcgcccgc
       61 gggcgcggag acgactacct gatccggatc aacacccagg cggcccgccg gaacctgagg
      121 atctacctgc ggccgctggg cgaacccaac cgctgcctca actactacgc gggcgttgac
      181 tgcgccggca acgtgcgagg tggcgactac tcatacggct ggtacaagca ggccttccgc
      241 cggatcgcga tcatcacccg cggcggcgcc aagcgcggtt tcatcaacgc ccggttgaag
      301 gaccttcgac tgcccaaggt tcagaacgtc ggccagtcca ggcgccttcc ccgccgcctg
      361 ccgaaggccc ccgtctcgct ggtctggagc ccgttgccgg ccggctcccc gaccgtccgg
      421 gccaacctcc cggcccggta ctggccgggc aaggactggg tcgactgggt cggcaccgac
      481 ttctacaacc gctacaacaa ctggaagcac ctcggccgct tctacaaaaa gtgggccctg
      541 ggcaagaaca agccgatggc cctgaccgag ttcgggctct ggggcagtga cagcccgtcc
      601 ttcatcaagc ggatcttcaa gttcacccgc aaccggaaga agatccggat gatggtctac
      661 taccaggact tcggcgagtc gaactccttc cggatccaga acttcccgca gggccgcaag
      721 gtcctgtcgc agttcctcaa caagcgcgac tacccgaagt tcgccccgaa gtacccccgc
      781 ttccggtagg cccccgagcg gccaggagcc acggtattcc acaaatactg tggaaaaccg
      841 ctggtcccgg gttgatcagc ggaaggccgg gatgccggtc agggccatgc cgatcgagag
      901 ggtgtggatc tcgtcggtgc cctcgtaggt gaagaccgat tcgaggttgt tggcgtggcg
      961 caggaccggg tactcgaggg tgatcccgtt ggcaccgagg atgccacggg cggtgcgggc
     1021 cacatccagg gccatccggg cattggcccg cttgcccatc gaaatgtgct ccggctcgat
     1081 ccggccctcg tccttgagct ggccgagcct gagggcgagc aggtagccct gctggacctt
     1141 ggtcgccatg tcggcgagct tggcctgggt gagctggaag gaagcgatcg gctcgtcgaa
     1201 ctgcttgcgg gtcatcgcgt aatcgagtgc cgcctcgaag catgagcggg cagcgccaac
     1261 gactccccag acgattccgt agcgggcttc gttcaggcag ctgagcgggc ccttcagcga
     1321 ctggacttcg ggcaggcggt tggcgtcggg gatccgcacg tcgtcgaagg ccagctccga
     1381 agtgaccgag gcccgcagtg aaagcttgtg gtgcatttcc ggggcggtga agcccttcat
     1441 gcccggctcg accaggaaac cctgaacgcc gtcatcggtc ttcgcccaga ccacggccac
     1501 gtcggcgatc gaaccggagg tgatccacat cttcgagccg ttgaggatcc agtcctcgcc
     1561 gtccttcttt gcggtggtcc gcatcgaccc ggggtcggag ccggagtccg gttcggtcag
     1621 gccgaagcag ccgatcagat caccggccgc catgcccggc agccagcgct gcttctgctc
     1681 ctcgctgccc cacttccaga tcgcgaacat ggccagtgaa ccctgcaccg agcagaagga
     1741 cctgagggcc gaatcgccgg cctccagctc caggcaggcg acgccgtagg cgaccgcgct
     1801 ggttccggcg cagccgtatc ccttcaggtg catgcccagc acgttgaggt cgccgagtgc
     1861 cttcggcacg gcggtgtcaa aggtcccgtt ctcgaagtgc tccgccaccc cgggcaggta
     1921 cttctcgttc acgtaacggg aaacggtctc cttgatgagg cgctcctcgt ccgtgaggag
     1981 ccggtcaatt tccagatagt cgcgcgggtt cacagaagac aatttaacga tccggccttc
     2041 gcccgaccgc gaaaccggcc ggtccgctca gggcagccgt ttcctttgct cgcaacccgg
     2101 ggccttcttc cggtccttgt tcgtgcctcc gtcgcattgg tccttgccac cgtctccacc
     2161 gctcagtttg tcgaagccgg aattgccgaa gagcctgtcc ttgccgtggc ct
//
LOCUS       DAOQMF010000307         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_32271_length_2206_cov_4.445374, whole genome shotgun sequence.
ACCESSION   DAOQMF010000307 DAOQMF010000000
VERSION     DAOQMF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32271_length_2206_cov_4.445374"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLV77_05890"
     CDS             <1..338
                     /locus_tag="PLV77_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNC15411.1"
                     /translation="RIRTEAGQGFTGKPVSGRRAAVEQSGGGHEQSAVYLDTVMLLGS
                     IANQQQGVDRWWWALGAVAASFTWFFLLGYGARLLGPLFARPGAWRVLDALIAIIMVG
                     LGAGLIIGG"
     gene            446..1393
                     /locus_tag="PLV77_05895"
     CDS             446..1393
                     /locus_tag="PLV77_05895"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC15412.1"
                     /translation="MSSAGMKRIPGAVVALTVAALVVAGCGGDGSSNKTEPAASQAAT
                     SQTLNNQENESADGFVPSFSEVKQDKGPVFGDGCLIHGDEPESGECAYGDTESSRNVV
                     VFGDSHALQWTPALIGIASQRGWRLTALLHANCTAAQVSVDENCDRWRENSLKRISEE
                     KPGLVIVASNTGPNMTVVSDGTKLDREAAGSSLEAGMARTFEDLKASGAEVTLMQDMA
                     MSKDYLPSECVAGHQDDPGQCTFEMDRPPELAYDLKAARQVGGVQVIDPLPKICPGYS
                     CYPVHGRYLKFRDRFHITATYSRLLAPWLDSRLQNPWEE"
     gene            1401..>2206
                     /locus_tag="PLV77_05900"
     CDS             1401..>2206
                     /locus_tag="PLV77_05900"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC15413.1"
                     /translation="MKHRGSSLLAGLLAIACLATVVMAGPATARFIPGLNRVRADKGE
                     VYRKGCLMGHNRVRSGPCRFGDRDSKKKVVLLGDSHAMQWGPGLIRLARKKQWQVIAL
                     TRASCPAALVHIDYYCDTWRRNSLRRIRLMRPGLVLVASAANSDAYSVFTGGGYLSRA
                     ASEQYLVDGMIRTLRRLRRWSKKTVVMQDQAVTPFSVTACLRINSPGNCGFRASRPRA
                     WSYDFKAARRVKKVPVIDLGRPLELRYSARSRLVRRAELHASRGWRLRLS"
BASE COUNT          366 a          738 c          743 g          359 t
ORIGIN      
        1 accggattcg caccgaggcc ggccagggtt tcacgggcaa gccagtgagt ggtcgccggg
       61 cggccgtcga gcagtccggc ggcggccatg agcaaagcgc cgtgtacctc gacaccgtga
      121 tgctgctcgg ctcgattgcc aaccagcagc agggcgtcga tcgctggtgg tgggcgctcg
      181 gcgcggtggc tgcctcgttc acctggttct tcctgctcgg ctacggggcc cggctgctgg
      241 gaccgctctt tgcccggccg ggtgcctggc gcgtgctcga cgccctgatc gccatcatca
      301 tggtcgggct cggcgccggc ctgatcatcg gcggctgacc cggccggcca ccggttccgg
      361 tccggcgaca cttgcccgca accgtcaggc ggtgtaggct cgcggacccc gcaggcaggg
      421 ggagaatcgg cacaggaggt aagcgatgtc ttcagcaggg atgaaaagaa tcccgggagc
      481 agtggtggcg ctaacggtgg cggcgctggt ggtcgccggt tgtggcggcg acgggtccag
      541 caacaaaacc gaaccggccg caagccaggc cgcaaccagc cagaccctga acaaccagga
      601 aaacgaatcc gccgacgggt tcgttccttc cttcagcgag gtcaaacagg acaagggccc
      661 ggtcttcggc gacggctgcc tgattcacgg cgatgagccc gaatccggtg agtgtgccta
      721 cggcgacacc gagtcgtccc ggaacgtagt ggtcttcggg gattcgcacg cgctccagtg
      781 gacgccggcc ctgatcggga tcgccagcca gcgcggctgg cgcctgaccg ccctgctcca
      841 tgcgaactgc accgcagccc aggtcagcgt cgacgaaaac tgcgaccgct ggcgggaaaa
      901 ctcgctgaag aggatcagtg aggaaaagcc cggactcgtc atcgtggcca gcaacaccgg
      961 ccccaacatg accgtcgtga gcgacggcac gaaactggac cgggaggcgg ccgggtcgtc
     1021 gctcgaggcc gggatggcca ggaccttcga ggacctcaag gcgagcggcg ccgaggtgac
     1081 cctgatgcag gacatggcga tgtcgaagga ctacctccct tcggaatgcg tcgccgggca
     1141 ccaggacgac ccgggccaat gcaccttcga gatggatcgc ccgccggagc ttgcctatga
     1201 cctcaaggcg gccagacagg tcggcggtgt ccaggtgatc gaccccctgc cgaagatctg
     1261 ccccggctac agctgctacc ccgtccacgg ccggtacctg aagttccgcg accgcttcca
     1321 catcaccgcg acctattcac gccttctcgc gccatggctt gactcgcggc tccagaatcc
     1381 ctgggaggag tgacccgcga gtgaagcatc gcgggtcgtc acttctcgcg gggctcctcg
     1441 cgattgcctg cctggccacc gtcgtcatgg ccggtccggc caccgctcgt ttcatccccg
     1501 gtctcaaccg ggtcagggct gacaagggcg aggtctaccg caagggctgc ctgatgggtc
     1561 acaaccgggt ccggtccggc ccctgccgct tcggggaccg cgactcgaag aagaaggtcg
     1621 tcctgctcgg tgattcccac gccatgcagt ggggcccggg gctgatccgg ctggccagga
     1681 agaagcagtg gcaggtgatc gccctgaccc gagccagctg cccggcggcg ctggtccaca
     1741 tcgactacta ctgcgacacc tggcgaagga actcactccg gcggatccgg ttgatgcgcc
     1801 ccggcctcgt gctggttgcc tcggccgcca acagcgatgc gtattcggtt ttcaccggcg
     1861 gtggctatct cagccgcgcg gccagcgagc agtacctggt cgacgggatg atccgcaccc
     1921 tgcggcgcct gcgtcgctgg tcgaagaaga cggtcgtgat gcaggaccag gcggtcaccc
     1981 cgttcagcgt gaccgcctgc ctccgcatca acagccccgg gaattgtggt ttccgggctt
     2041 cgcgtccgcg ggcctggtca tatgacttca aggcggcgcg ccgggtgaag aaggttccgg
     2101 tcatcgatct gggccgtcca cttgaactga ggtattccgc ccgcagcagg ctggtgcgac
     2161 gcgctgaact tcatgcttcc cgcggctggc ggctccggct ttccag
//
LOCUS       DAOQMF010000308         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_32283_length_2206_cov_3.178057, whole genome shotgun sequence.
ACCESSION   DAOQMF010000308 DAOQMF010000000
VERSION     DAOQMF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32283_length_2206_cov_3.178057"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..464
                     /locus_tag="PLV77_05905"
     CDS             168..464
                     /locus_tag="PLV77_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15414.1"
                     /translation="MSIVIWFTVALALWHFTIFVPDRFWQGIIGALIGCVLGGLISGA
                     IVELILGNGLADTDIITFLAAVPGTLIGAWVIYRIGVSQGTEPIEAGSEPAEKV"
     gene            544..>2206
                     /gene="recJ"
                     /locus_tag="PLV77_05910"
     CDS             544..>2206
                     /gene="recJ"
                     /locus_tag="PLV77_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNC15415.1"
                     /translation="MATVLTRRGFGDPDAAARFLGAGESHDPFGFEGMAEVVARVRAA
                     IEAGEKITVYGDFDCDGVCATSILVGALRELGGDCDWFIPDRIDGYGLNPDSIRRLHQ
                     RGTSLVITVDCGVTSVGEVALARELGLGIIVTDHHQAETALPECPILHPEVSGYPFPS
                     LCGAAVAAKLASALRAGTSSGPERDEADLDLVALATVADVMPLVGENRHLVREGVRVA
                     RRARRVGLAALMADCRIEPSRLSSEDFGFRLGPRINAAGRLYRADAGVELFLADSRER
                     AGEIARELASVNAERRTVEREVEGLARAELKALGDPGPAVVVAGEGWHQGVVGIVASK
                     LVKATGRPSVVISIDGETGRGSARSVPGLDLHAAIGETADLLETFGGHAAAAGLSIRT
                     DRIDDFRSALGRAVASRIGTEPAEKAIEFDAVAGGADLGLDLAEEIEKLQPFGNGNPP
                     VRLFLPGARIEDLQEMGEGKHCRFAVCSGSHRATGVAFGRTSFGLAEGERADVIAELG
                     INHWNGSIEPQLRVTEVSRLPAAEPLAECEPDEWWQRFETALTRPL"
BASE COUNT          343 a          718 c          794 g          351 t
ORIGIN      
        1 gacctcgagg tcgcccgcaa ggattcaggc aacggagccg agagcaaggc aggcgaggaa
       61 ggcgcagtcg aggaagcggc cccgtcgcaa ggcgggccga acctctcgcc ccgtgaccgg
      121 cgccggctgg agcggcagaa agccaaggaa agcggagggg aagcctgatg tcaatcgtta
      181 tctggttcac cgtcgcgctc gcgctctggc acttcacgat ctttgtgccc gaccgtttct
      241 ggcagggcat catcggcgcc ctgatcggct gcgtgctcgg cggattgatc tccggtgcga
      301 tcgtcgagct gatactcggc aacggccttg ccgacaccga catcatcacc ttcctggccg
      361 cggtgccggg aacgctgatc ggcgcctggg tgatttaccg gatcggtgtt tcccagggca
      421 ccgaaccgat tgaagccggc tccgagccgg cggaaaaggt ctagcgggat tcgtttccgg
      481 gccgagcctt acccggtcga caccgccctc gaactggagc gggcaaccgg gctgagccgg
      541 cctgtggcga ccgttcttac ccgccgcgga ttcggcgatc cggacgccgc cgcccgcttt
      601 ctcggggccg gcgaaagcca tgatcccttc gggttcgagg gcatggccga ggtcgtggcg
      661 agggtccggg cggcgatcga ggccggcgag aagatcaccg tttacggcga cttcgactgc
      721 gacggggtct gcgcgacttc gatcctggtc ggtgccctgc gtgaactggg cggtgactgc
      781 gactggttca tccccgaccg gatcgatgga tacggcctga acccggattc gatccgccgg
      841 cttcaccagc gcggtacctc gctggtcatc accgtggact gcggcgtgac ctcggtcggg
      901 gaggtggccc tggccaggga gctgggcctt ggaatcatcg tgaccgacca ccatcaggcg
      961 gaaacggccc tgccggagtg cccgatcctt cacccggagg tcagtggcta tccgttcccc
     1021 tcgctttgcg gagcggcggt cgccgccaaa ctcgcctcgg cgctcagggc cggcacctcg
     1081 tcgggcccgg agcgcgacga ggcagacctc gatctggtcg ccctcgcgac cgtcgccgac
     1141 gtgatgcccc ttgtcggcga gaaccgtcac ctggtccgcg aaggcgtcag ggtcgcccgt
     1201 cgcgcccggc gggtcggcct ggccgccctg atggccgact gcaggatcga accctcgcga
     1261 ctcagctcgg aggatttcgg cttccggctc ggcccgagga tcaacgccgc cggtcgcctc
     1321 taccgggccg acgccggggt ggagctcttc ctcgcggact cgagggaaag ggccggcgag
     1381 atcgcccgcg agctggccag tgtcaacgcc gaacgacgca cggtcgagcg cgaagtggag
     1441 gggctggcca gggccgagct gaaagcgctc ggcgacccgg gcccggcggt cgtcgtggcg
     1501 ggggagggct ggcatcaggg cgtggtcggg atcgtcgctt cgaaactggt caaggccacc
     1561 ggccggcctt cggtggtgat ctcgatcgac ggtgaaaccg gcaggggttc ggccaggtcg
     1621 gttcccggtc tggaccttca cgccgcgatc ggggagacgg ccgacctgct ggagactttc
     1681 ggcggccacg ccgccgccgc cggtttgagc ataaggaccg accgaattga cgattttcga
     1741 tcggctctgg gccgggcggt cgcgtcacgg atcggcaccg aacccgccga gaaggcgatc
     1801 gagtttgacg ccgtggccgg gggtgccgac ctggggctcg acctggccga ggagatcgag
     1861 aagctgcagc cgttcgggaa cggcaatccg ccggtcaggc ttttcctgcc cggtgccagg
     1921 atcgaggatc tgcaggagat gggcgaaggg aagcactgcc gcttcgccgt ctgttcgggc
     1981 tcccatcgcg caaccggcgt ggccttcggc cggacctcgt tcggactggc agaaggggaa
     2041 agggccgacg tgatcgccga actggggatc aaccactgga acggctcgat cgaaccgcag
     2101 ctgcgggtca ccgaggtttc ccggctcccg gccgccgaac cgctcgcgga gtgtgagccg
     2161 gacgagtggt ggcaacgctt cgagaccgca ctgacgaggc ccctgg
//
LOCUS       DAOQMF010000309         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_32678_length_2190_cov_4.046838, whole genome shotgun sequence.
ACCESSION   DAOQMF010000309 DAOQMF010000000
VERSION     DAOQMF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32678_length_2190_cov_4.046838"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PLV77_05915"
     CDS             <1..1287
                     /locus_tag="PLV77_05915"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578774.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNC15416.1"
                     /translation="NAQATVLAPTGTISFLMDCDTTGIEPDFSLVKFKELVGGGNMTI
                     VNKSVPLALRTLEYSESEISQIEAYINEHGTIIGAPALKEEHLPVFDVAVGERAISHT
                     GHIDMMAATQPFLSGAISKTVNMPNSATVEDIADAYTRGWEGGLKALAIYRDGSKTAQ
                     ALRTDAQKDKEKEAEAEAAPNEPVRHRMPKTRDSVTHKFSVGGHEGYITAGKYTDGSV
                     GEIFLTDIGKEGSTMRGMMNAFATAISLGLQYGVPLEVFVSKFSYMRFDPEGITGNPD
                     IPFAKSMPDYIMRWLASQFIDDPDFLEEQGIMTQEVRNRKEAENAGINLANGSGNGSP
                     AGNGGNGNGNGGNGNGGSAPSASTESAAPVADTGGSAVPAGTALTDDVPVVPARLHFT
                     PGAAELGPACDQCGGMMQRTGACYTCSSCGNNTGCG"
     gene            1517..1750
                     /locus_tag="PLV77_05920"
     CDS             1517..1750
                     /locus_tag="PLV77_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15417.1"
                     /translation="MGDLVRRKWHLMLIGLIAGAVIVGLVIGPLVVKVFGTVAGVYLI
                     LFLILRRRKGPDGKINPWYASNGRQKGGGIFPF"
     gene            1832..>2190
                     /locus_tag="PLV77_05925"
     CDS             1832..>2190
                     /locus_tag="PLV77_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC15418.1"
                     /translation="MATTSDKIAASPGTGTARTRSTGSRSLSTAEERREELITAAIPV
                     FAEKGYHAAPTTEIAKKAGISQAYLFRLFPTKEELFVAAVERSTGRMVETLAAVASEA
                     EKNGIEPGDAMGLAYGDL"
BASE COUNT          425 a          717 c          690 g          358 t
ORIGIN      
        1 aacgcccagg cgacggtgct ggccccgacc ggcacgatca gcttcctgat ggactgcgac
       61 acgaccggca tcgagccgga cttctcgctg gtcaagttca aggaactggt cggcggcggc
      121 aacatgacca tcgtcaacaa gtccgtgccg ctggcccttc gcacgctcga gtactcggaa
      181 tccgagatct cgcagatcga ggcctacatc aacgagcacg gcacgatcat cggcgccccg
      241 gccctgaagg aagagcatct gccggtcttc gacgtcgcgg tcggcgagcg ggcgatcagc
      301 cacaccggcc acatcgacat gatggcggcg acccagccgt tcctctccgg cgccatctcg
      361 aagacggtca acatgccgaa ctcggcgacc gtcgaggaca tcgccgacgc ctacactcgc
      421 ggctgggaag gcggcctcaa agccctcgcg atctaccgtg acggttccaa gaccgcccag
      481 gcgctccgca ccgacgccca gaaggacaag gagaaggaag cggaagccga ggcggccccg
      541 aacgagccgg tccgtcaccg gatgccaaag acccgcgact cggtcaccca caagttctcg
      601 gtcggcggcc acgagggtta catcacggcc ggcaagtaca ccgacggctc ggtcggcgag
      661 atcttcctga ccgacatcgg caaggaaggc tcgaccatgc ggggcatgat gaatgccttc
      721 gcgaccgcga tctcgctcgg cctccagtac ggggtgccgc tcgaggtatt cgtgagcaag
      781 ttcagctaca tgcggttcga cccggaaggg atcaccggca acccggacat ccccttcgcc
      841 aagtcgatgc cggactacat catgcgctgg ctcgcctccc agttcatcga cgatcccgac
      901 ttcctcgagg agcaggggat catgacccag gaggtccgca accgcaagga agccgagaac
      961 gccggcatca acctggccaa cggctccggc aacggcagcc cggccggcaa cggtggcaac
     1021 gggaatggca acggcggcaa cggcaacggt ggctcggccc cgtccgcatc aaccgagtcg
     1081 gcggccccgg tcgccgatac cggcggctcc gcggtcccgg ccggcacggc gctcaccgat
     1141 gacgtcccgg tggtcccggc caggctccac ttcactcccg gggcagcaga gctgggcccg
     1201 gcctgtgacc agtgcggagg catgatgcag cgaaccggcg cctgctacac ctgctcgtcc
     1261 tgcggcaaca acaccggatg tggatgaacc acccaggttg agttcaactc gtttcgaaag
     1321 ggccccggag agatccgggg cctttttcct gggcagaccc ggattcatat gcaatacgcg
     1381 tggctttccg tcctcgccag gtcagccgcc cggatcccat tttgaggtgg gtatgaaaag
     1441 tgtctatatg aacgaatttc atacccacct cggccacttg gccccggttg ccgggatccg
     1501 ggattaagat cgcgatatgg gagatctggt ccggaggaag tggcacctga tgttgatcgg
     1561 cttgattgcg ggggcggtga tcgtcggact ggtaatcggg ccgctcgtgg tcaaggtgtt
     1621 cggcaccgtc gccggcgtct acctgatcct ctttctgatt ctcaggcgcc gcaagggacc
     1681 cgacgggaag atcaatccct ggtacgccag caacggccgc cagaaaggcg gcggcatctt
     1741 tcctttctga ggtcggatgt caggtggctt tcgcagtgaa ttcaggccgt cgacttgaca
     1801 aatggtgtga gtgagtgcat actcacacct gatggcaacg acttcggaca agatcgcagc
     1861 gtccccgggg accgggacgg ccaggacccg ctcgacggga tcccgctcgc tttcgaccgc
     1921 tgaagagcgc cgtgaggaac tgattacggc ggcgattccg gtctttgccg agaagggtta
     1981 ccacgccgcc ccgaccaccg agatcgcgaa gaaggccggc atttcgcagg cctacctttt
     2041 ccggctcttc ccgaccaagg aggaactctt cgtggccgcg gtcgaacggt cgaccgggcg
     2101 catggtcgag actctcgccg ccgtggcttc ggaggccgag aagaacggga tcgaacccgg
     2161 cgatgccatg ggcctcgcct atggtgacct
//
LOCUS       DAOQMF010000310         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_32762_length_2187_cov_3.507505, whole genome shotgun sequence.
ACCESSION   DAOQMF010000310 DAOQMF010000000
VERSION     DAOQMF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32762_length_2187_cov_3.507505"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLV77_05930"
     CDS             complement(<1..639)
                     /locus_tag="PLV77_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15419.1"
                     /translation="MTGRRTILFSLIALTMGLGLGAQAQAAKPKADVVFSNGGRILTM
                     KADGTERKVLFGKDRLPRNDGLGAVEPSVSPDGTKITFGFRRLDRYDSLFDVWVMNFD
                     GTGARKLLASNRKQRYADPEFTAGGRIIAASFRENSSLAQARIFTMDTNGKSRKVMLR
                     LKQRQRPWEGWKSLAEPSLSPDGKHLLYLLDPGYDGTSFEEGYGASLRVLNID"
     gene            complement(642..>2187)
                     /locus_tag="PLV77_05935"
     CDS             complement(642..>2187)
                     /locus_tag="PLV77_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15420.1"
                     /translation="WPGAGQATTPPLAPLAEIVFENGGRIVSIKADGSGRKVLTRKGT
                     DLLRRNYLADIHYFGDSQPAISPDGEKLAFVRYRPAAHSQQVMLADRDGWGATAVKGI
                     NAEIQSLDFMPDGRLLLTTWDYRYGYSRTTETFRILALDPATGKKKVLVSKRFFGNYN
                     DDTLQAQEVSPDGKSLLYTRDFGENDDGYDVVQLRIRNLVSGAERVLVTGAEAGSFSP
                     DGERVAFTKMCVDTEGYAHCAHSGDGRTGVWTIGVDGSDRQLIVTGDGGYSSPDWGPG
                     GGYIIFASSRNFPEERQDANEIYSVRSDGECLTWLTNGSPESVMPAWAPETEVRTDPG
                     AKCGAAGRKPLAEARPPRAIDTDPRPRYWAGPIVANRLLSKVDGYAQDEFGSPIYRYG
                     DCPFFDSSDCRRPITIEIAFACGGLGEVRVPPRGREQVIRRRGVRVTRHWTARGKFVT
                     TTLRTGLVDIGIGQSMLDVEDIGGRASTFSDHLAFIDNLRPVGKSLDPGSKLPPAATV
                     KCQGRR"
BASE COUNT          350 a          712 c          726 g          399 t
ORIGIN      
        1 gtcgatgttc agcacccgaa gcgaagcccc gtagccctcc tcgaaagaag tcccgtcgta
       61 tcccgggtcg agcagataga gcagatgctt cccgtcgggc gagagcgaag gttcggccag
      121 gctcttccag ccctcccagg gacgctggcg ctgtttcagc cgcagcatca ccttgcggga
      181 tttgccgttc gtatccatgg tgaagatccg ggcctgggcc aggctggagt tctcgcggaa
      241 ggaggcggcg atgatccggc cacctgccgt gaattccgga tcggcgtagc gttgcttcct
      301 gttcgaggcc agcagcttgc gggctccggt gccgtcgaag ttcatcaccc agacatcgaa
      361 cagagagtca tatctgtcga gccgcctgaa gccaaaagtg atctttgtcc cgtccgggga
      421 aaccgaaggc tcgacggcgc cgagcccgtc gtttcgggga aggcggtcct tgccgaagag
      481 gactttccgt tcggtgccgt cggccttcat cgtgaggatg cggccgccgt tgctgaacac
      541 cacgtccgcc ttcggcttcg ccgcctgagc ctgggcgcca aggccgaggc ccatggtgag
      601 ggcgataagc gaaaagagga tcgtgcgacg gccggtcatc ttcacctccg gccctggcac
      661 ttgaccgtgg cggcgggagg cagcttcgaa ccggggtcga gggacttgcc gaccggtcgc
      721 agattgtcga tgaaggccag gtggtccgag aaggtcgagg ccctgccgcc gatgtcctcg
      781 acgtcgagca tcgactggcc gatcccaatg tcaaccaggc cggtccgcag ggtggtagtg
      841 acaaattttc ctcgcgctgt ccagtgccgg gtcaccctga ctccgcgtcg ccggatcact
      901 tgctccctgc ctcggggtgg caccctgacc tcgccgaggc caccgcaggc gaaggcaatt
      961 tcgatcgtga tcggtcgccg gcaatccgag ctgtcgaaaa agggacagtc gccgtagcgg
     1021 tagatcgggg aaccgaactc gtcctgggcg taaccgtcga ccttggagag cagccggttc
     1081 gcgacgatcg gcccggccca gtagcgcggg cgggggtcgg tgtcgatcgc cctcggcggc
     1141 cgcgcctcgg ccagcggttt gcgtccggcc gcaccgcatt tcgcaccggg gtcggttcgc
     1201 acctcggtct ccggcgccca ggcgggcatg accgactccg gtgatccgtt ggtcagccag
     1261 gtcaggcatt cgccgtcgga gcgcaccgag tagatctcgt tcgcgtcctg ccgctcctcc
     1321 gggaagttcc ggctgctggc gaagatgatg tagccgccgc ccgggcccca gtcgggcgag
     1381 ctgtacccgc cgtccccggt cacgatcaac tggcgatcgg aaccgtcgac cccgatcgtc
     1441 cagaccccgg tccttccgtc accggaatga gcgcagtggg cgtaaccctc ggtgtcaacg
     1501 cacatcttcg tgaaggcgac ccgctcgcca tcgggagaga acgagcccgc ttcggcgccc
     1561 gtcaccagta cccgctctgc cccgctgacc aggttccgga tccgcagctg gaccacgtcg
     1621 taaccgtcat cgttctcgcc gaagtcacgg gtgtagagca gcgacttgcc gtcggggctt
     1681 acttcctggg cctggagcgt gtcgtcgttg tagttgccga agaacctttt cgagacgagg
     1741 acctttttct tcccggtcgc ggggtcgagc gcgagaatcc ggaaggtctc ggtggtccgg
     1801 ctgtagccgt agcggtaatc ccaggtggtg aggaggagcc ggccgtcggg catgaagtcc
     1861 agcgactgga tttcggcgtt tatgcccttg accgcggttg ccccccaacc gtcccggtcg
     1921 gccagcatca cctgctgcga atgtgcggcg ggccggtacc tgacgaaggc gagtttttcg
     1981 ccgtccgggg aaatcgccgg ctggctgtcg ccgaagtaat ggatgtcggc cagatagttg
     2041 cggcggagca ggtcggtgcc cttgcgggtc agaaccttgc gtccggatcc gtcggccttg
     2101 atcgaaacga tccggccacc gttctcgaaa acgatctccg ccagcggcgc cagcggcggc
     2161 gtcgtcgcct gcccggcccc cggccaa
//
LOCUS       DAOQMF010000311         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_32987_length_2179_cov_3.104049, whole genome shotgun sequence.
ACCESSION   DAOQMF010000311 DAOQMF010000000
VERSION     DAOQMF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32987_length_2179_cov_3.104049"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLV77_05940"
     CDS             <1..282
                     /locus_tag="PLV77_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15421.1"
                     /translation="APSGIGGSLTARMLGRISPAIRDGYMTRGEREGADEALLDRHHR
                     EPGLSPSILQVRPPADSAVPSRLESDMKVVRTGLEAGRRAVHQLFSTES"
     gene            complement(279..>2179)
                     /locus_tag="PLV77_05945"
     CDS             complement(279..>2179)
                     /locus_tag="PLV77_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15422.1"
                     /translation="SVGQLPGEDEKKANPVDIAIDDEDNVYVLDQIEQMVRKYTADGS
                     LLDQWSAAVPCCLDVEGITVEAGSVFVISGYDVYRYTPSGVLLDSWRGFDSYPEWEQP
                     QIRGDGEGHLLIGSGRFLDEFKANKSIYKFEPDGTMISQWTTPAQGWVYYSQDEWGIY
                     ENLADIDGFDAVVPMPDGNPWTAAGGRIARYGSSGEFGDFLDFDRKRFPNALALAPDG
                     GLLYSWAEIPNYPEVSGSGVAKLDPDLDTTRVWTESDFPKYASNTWDGKFGHALGLTV
                     RTDGVVGGYDTNMDRAQLFDSDGTFIKRIEGPQPNIDSDETPEFLLPEAGGAEFFDPG
                     SGRLSRYDADGDLVDTITLETTGNFEDIALDPEGGYIVLTTVTDRVGQVTFFDQQGEQ
                     TSGFKSVRSLASYNGSSLHVEPGAGGIYVQGTGYVEKYSRAGKRLATGVVRGKGCDSA
                     YNYDGLATDLAGNVYLVSEGSQAPMVIKFNRSLKRLWEEKVPKVRWYTGFSGDLDIGP
                     DGSIFMESDKGIMRYDQSKASARPTHEACPKPFHLLRIARTPGRRWAIAAVHVPSSGL
                     LTVSGRKVRTAKRRVGDYGDYGLAIRPKARFTRAARPGFRRFPVTITYKGRHSGKIHT
                     AVKLRVGR"
BASE COUNT          369 a          718 c          687 g          405 t
ORIGIN      
        1 gctccctccg ggatcggcgg gagcctcacc gcccggatgc tcgggcggat cagcccggcg
       61 atccgcgacg gatacatgac gcggggcgag cgggaaggag ccgacgaggc cctgcttgac
      121 cgccaccacc gtgaacccgg actcagcccg agcatcctgc aggtccggcc accggcagat
      181 tccgccgtgc cttcacggct tgaatcggac atgaaagtcg tgcggaccgg actcgaagcg
      241 ggccggcggg ctgttcacca gctcttctcg accgagagct agcggccgac ccgaagcttc
      301 actgcggtgt ggatcttccc ggagtgccgg cccttgtagg tgatcgtgac cggaaagcgc
      361 cgaaaacccg gccgggccgc ccgggtgaag cgggccttcg ggcggatcgc caggccgtag
      421 tcgccgtaat ccccgacccg gcgtttcgcg gtcctgacct tgcggccgct gacggtcaga
      481 aggcccgagc tcgggacatg aaccgcggcg atcgcccatc tgcgccccgg agtcctggcg
      541 atccgcagca gatggaaagg cttcggacag gcctcatggg tcggccgggc gcttgccttt
      601 gactggtcgt agcgcatgat tcccttgtct gactccatga agatcgagcc atccgggccg
      661 atgtccaggt ccccggaaaa ccccgtgtac cacctgacct tgggcacctt ctcctcccaa
      721 agccgtttca gggagcgatt gaacttgatc accatcggcg cctggctgcc ctcgctgacg
      781 agatagacgt tccccgccag gtcggtggcg aggccgtcgt agttgtaggc cgagtcgcat
      841 cccttgcccc gcacgactcc ggtcgcgagg cgcttgccgg cccgcgagta cttttcgacg
      901 tagcccgtac cctggacata gataccgccg gctcccggtt cgacatgcag gctggatccg
      961 ttgtagctgg cgagcgaacg gaccgacttg aatccggaag tctgctcacc ctgctggtcg
     1021 aagaaggtga cctggccgac ccggtcggtg accgtggtca aaacgatgta gccgccttcc
     1081 ggatccagcg cgatgtcctc gaagttgccg gtcgtctcca gggtgatggt gtcaaccaga
     1141 tcaccgtcgg cgtcgtagcg gctcagcctg cccgagcccg gatcgaagaa ctctgcgccg
     1201 cccgcctccg gcaggaggaa ttccggggtc tcatccgaat cgatgttggg ctgcggtcct
     1261 tcgatccgct tgatgaaagt cccgtccgag tcgaatagct gggcccggtc catgttggtg
     1321 tcatacccgc caaccactcc gtcggtccgg acagtaaggc cgagggcgtg gccgaacttg
     1381 ccgtcccagg tgttgctcgc gtacttggga aaatcggact cggtccagac ccgggtcgtg
     1441 tccagatccg gatcgagttt ggccacgccg gaacctgaca cctcggggta gttcggaatc
     1501 tccgcccagg aatagagcag cccgccgtcc ggggccagtg ccagtgcgtt cgggaagcgc
     1561 ttgcggtcaa agtcaaggaa gtcaccgaac tcgccggatg atccgtatct ggcaatccgg
     1621 cctccggcgg cggtccacgg gtttccgtcg ggcatcggca ccaccgcgtc gaaaccgtcg
     1681 atgtcagcca ggttctcgta gatgccccac tcgtcctggg agtaatagac ccagccctgg
     1741 gcgggggtgg tccactgcga gatcatcgtg ccgtcgggtt caaacttgta gatcgacttg
     1801 ttcgccttga attcgtcgag aaacctgccg ctgccgatca gcaggtggcc ttcaccatcg
     1861 ccccggatct gcggctgctc ccattccggg tacgagtcaa accccctcca gctgtccagc
     1921 aggaccccgg acggcgtgta gcggtaaacg tcgtagccgc tgatcacgaa aaccgaaccg
     1981 gcttccacgg tgatgccttc gacgtcaagg cagcagggaa cggcggcgct ccactgatcc
     2041 agcagggagc cgtctgccgt gtacttcctg accatctgtt cgatctggtc gaggacgtag
     2101 acgttgtctt cgtcgtcgat cgcgatgtcg accgggttgg ccttcttctc gtcttcgcca
     2161 ggcagttgcc cgacgctcc
//
LOCUS       DAOQMF010000312         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_33186_length_2172_cov_3.375059, whole genome shotgun sequence.
ACCESSION   DAOQMF010000312 DAOQMF010000000
VERSION     DAOQMF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33186_length_2172_cov_3.375059"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2172)
                     /locus_tag="PLV77_05950"
     CDS             complement(<1..>2172)
                     /locus_tag="PLV77_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC15423.1"
                     /translation="DHVDVINMSLGSDFGSVQDGDSVASNAAVQMGVSVVASAGNSGD
                     ISDITGSPGDASQVLSVASSVDAESKVDGAEVTIDGNQDLYAITRSGRYDWKTGPDLS
                     GPVVAAPVENETACAPYTGTPFTGKVVLVKWHDAVPECGSITRGDNLAAAGAIGFIFG
                     SDAETFSAGINGDDAIPGVLMAKSGADAIRDALDNSLAVSVDGTEVNAVTRSFPDDND
                     KISSFTSRGIHATGNVKPDVTAVGSSVFSTAVGTGDDGVSFSGTSMASPMVAGLAALV
                     RQANPGWTPLQVKADIMNTAAHDLFVDGSANPSSDRYGPPRVGSGRIDAALATGNRVL
                     AYDPANGAVSVSFGPVAASAPVTLSREVKVENQSSFPVTYDLAYDPINEVPGAEFSVT
                     PSQVSLDPGRSTMVSVSLAIEDPSQLTKAVDPTAGRFSSEGDPRETLAEAFGRLLLEP
                     TGPGTTLRVPVYASPRPASDMGQPASLLIHRSSATAGDPEQTASLELAGQGLGSGPGE
                     NGVGDGDPDNDIFSIAAGFELQATSGESPECDEEIETSCWRLPEEKYADLKLVGYTSN
                     APLVADPDQQLAYFAAAVQAPWAIPADKAAIQFDLDVDGDGTPDLFLYNDRLGEDDVF
                     VSTLLDPSRPEGQRIIDQQLVNGRFGDIDTALYDSDALVLPVSLAALADYGIDADNPR
                     VSYGVETYSYSSDQAIDMIGVNAESGDLENPLSANLFEPGIL"
BASE COUNT          350 a          709 c          744 g          369 t
ORIGIN      
        1 caggatgcct ggctcgaaga gattcgcgct gagcggattc tcgaggtcgc cgctttcggc
       61 attgacgccg atcatgtcga tcgcctgatc ggaggagtac gagtaggtct caaccccgta
      121 tgaaacccgg gggttgtctg cgtcgatgcc gtaatcggcc agcgcggcca gtgaaacggg
      181 cagcaccagc gcgtcgctgt catagagcgc ggtgtcgatg tccccgaagc gcccgttgac
      241 cagctgctgg tcgatgatcc gctggccctc cggccgggac ggatcgagca gggtactgac
      301 gaagacatcg tcctccccga ggcggtcgtt gtagaggaag aggtccggcg tgccgtcccc
      361 gtcgacgtcg aggtcgaact ggatcgcggc cttgtccgcc gggatcgccc agggcgcctg
      421 aaccgcagcc gcgaagtacg ccagctgctg atccgggtcg gcgaccagcg gggcgttcga
      481 ggtgtagccg accagcttca ggtctgcgta cttctcttcc ggcagccgcc agcaggaggt
      541 ctcgatctcc tcgtcgcatt cgggcgactc cccgctcgtc gcctgcagtt cgaagcccgc
      601 ggcgatcgaa aatatgtcgt tgtccgggtc gccgtccccc actccgtttt caccggggcc
      661 gctgccgagg ccctggccgg ccagctcgag gctcgcggtc tgctcgggat cgccggcggt
      721 ggcggagctg cgatgaatca gaagcgaagc gggctggccc atgtccgagg ccggccgggg
      781 agatgcgtaa accgggaccc gtagggtcgt gccggggccg gtcggctcga ggagcagccg
      841 cccgaacgcc tcggccaggg tctcgcgcgg gtcaccctcg gagctgaagc ggccggcggt
      901 cggatcgacc gccttggtca gctgggaggg gtcctcgatc gcgaggctga ccgagaccat
      961 ggtcgacctg cccggatcca ggctgacctg ggaaggcgta accgagaatt cggcgcccgg
     1021 aacctcgttg atcgggtcat aggcgaggtc gtaggtcacc gggaaggaag actggttctc
     1081 caccttgacc tcacgactga gggtcaccgg tgcgctggcg gcgaccgggc cgaaggacac
     1141 actgaccgct ccgttggccg ggtcgtaggc cagcaccctg ttgccggtgg cgagtgccgc
     1201 gtcgatccgg ccggaaccga cccggggcgg gccgtagcga tccgaggacg gattggccga
     1261 accgtcgacg aagaggtcgt gcgcagccgt gttcatgatg tccgccttga cctggagcgg
     1321 agtccagccc ggattggcct gccggaccag ggccgccagc ccggcgacca tcggcgaagc
     1381 catcgaggtg ccggagaagc tgaccccgtc gtcacctgtg ccgaccgcgg tcgagaagac
     1441 cgagctgccg accgcggtca cgtccggctt gacgttgccg gtcgcgtgaa ttccacgcga
     1501 ggtgaagctc gagatcttgt cgttgtcatc agggaagctc cgggttaccg cgttgacctc
     1561 ggttccatcg acgctcaccg ccagcgagtt gtcgagcgcg tcgcggatcg cgtccgcccc
     1621 cgatttcgcc atcaacacgc cggggatcgc gtcgtcaccg ttgattccgg cggagaaggt
     1681 ttccgcgtcg ctgccgaaga tgaagccgat cgctccggcc gcggcgaggt tgtcgccacg
     1741 ggtgatcgaa ccgcattccg gcaccgcgtc atgccatttg acgagcacca ccttgccggt
     1801 gaacggggtc ccggtgtacg gggcgcaggc ggtttcattt tccaccggag cggccaccac
     1861 cggcccgctg aggtcgggac ccgtcttcca gtcgtaacgg cccgaccggg tgattgcgta
     1921 cagatcctgg ttcccgtcga tcgtgacctc ggcgccatcc accttcgact cggcgtcaac
     1981 gcttgaggcc acgctcagaa cctgcgaggc atctccgggc gagccggtga tgtcagagat
     2041 gtcaccggag tttccggccg aggcaaccac gcttaccccc atctgaaccg cggcgttgga
     2101 ggcgaccgaa tccccgtcct gaaccgatcc gaaatcggag ccgagcgaca tgttgatcac
     2161 gtcgacatgg tc
//
LOCUS       DAOQMF010000313         2168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_33311_length_2168_cov_3.673450, whole genome shotgun sequence.
ACCESSION   DAOQMF010000313 DAOQMF010000000
VERSION     DAOQMF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33311_length_2168_cov_3.673450"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLV77_05955"
     CDS             <1..394
                     /locus_tag="PLV77_05955"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNC15424.1"
                     /translation="NYWSMRVMAYTGTLMFLLMALGALFYWRGTLERRRRFLWAAVGG
                     IALPYVAATAGWVLTEMGRQPWIVQGLLKTADANSPAVSSGQIIASLSVFVLLYTCLA
                     VADVTLMRRYARVDPPGGDDSKLEAEPA"
     gene            391..1452
                     /gene="cydB"
                     /locus_tag="PLV77_05960"
     CDS             391..1452
                     /gene="cydB"
                     /locus_tag="PLV77_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNC15425.1"
                     /translation="MSLETVWFILIAVLWSGYFVLEGFDFGVGMLLPVLGRSESDRSA
                     MLNTIGPIWDGNEVWLIVAGGATFAAFPLWYATMFSGFYLALLAILVCLILRVVSFEW
                     RGKGDTPRWLGFWTWVNAVTSCLAAVIWGVALSNLLQGLPIDSSQEFTGNFLDLFSPF
                     TVFAGLAFCLLFLFHGAGFLSLRTVGDLGQRSARMARRLAAPALLAGILFMVWCVATA
                     VAENDRGVLGPLVPAVAGSLALCGAALAARARRPGLSFTLGAAGIVGVIATLFTGLYP
                     RVMVSSTDLANSLTTGNAASSHYSLTVITIVAGCLLPVVLAYQSWSYWVFRHRVSEDG
                     FEQAGNPIEAIRQRTEKRR"
     gene            1500..>2168
                     /locus_tag="PLV77_05965"
     CDS             1500..>2168
                     /locus_tag="PLV77_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC15426.1"
                     /translation="MSPAAARTTGGKPDSLPTAAVEDYAKAIYSLTGWGEETASTNDL
                     AERLGVKAGSVSAMIKKLDEAGLVERVPYHGVRLTADGTRVAMAVLRRHRLLELFLAE
                     VLDVPWDRVHAEAEVLEHALSEDLTDLISTKLGDPDFDPHGDPIPDRDLAIVETETTA
                     LADLAPGDSARFVRVSDSNPEMLAYLSRCGIAVGDQLELIERQPFDGPITVRIDSDSH
                     VLGLT"
BASE COUNT          311 a          740 c          706 g          411 t
ORIGIN      
        1 caattactgg tcgatgcggg tgatggccta caccggcacc ctgatgttcc tgctgatggc
       61 gctgggggcg ctgttctact ggcggggaac cctggaaagg aggcggcgtt tcctctgggc
      121 cgcggtcggc gggatcgcgc tgccctatgt cgcggccacg gccggctggg tgctgaccga
      181 aatgggccgg cagccctgga tcgttcaggg cctgctgaag accgcggacg cgaactcccc
      241 ggccgtgtcc agcggccaga tcatcgccag tctctccgtc ttcgtgctgc tctacacctg
      301 cctggctgtc gccgacgtga ccctgatgcg ccgttacgcg agggtcgacc cgccgggcgg
      361 cgacgattcg aaactggagg ccgaaccggc atgagcctgg aaacggtctg gttcatcctg
      421 atcgccgtgc tctggagcgg ctacttcgtg ctggaagggt tcgacttcgg ggtggggatg
      481 ctgctgcctg tactgggccg gagcgagtcc gaccggagcg cgatgctgaa cacgatcggg
      541 ccgatctggg acggcaacga ggtctggctg atcgtcgccg gcggcgcgac cttcgccgcc
      601 tttccgctct ggtacgcgac catgttctcc ggcttctacc tggccctgct ggcgattctg
      661 gtctgcctga tcctccgggt cgtctccttc gagtggcgcg gcaagggcga tacgccccgc
      721 tggctcggct tctggacctg ggtgaacgcg gtcaccagtt gcctggccgc ggtgatctgg
      781 ggcgtcgctc tctcgaacct cctccagggt ctgccaatcg attcctcgca ggagttcacc
      841 ggcaacttcc ttgacctctt ctcacccttc acggtcttcg ccgggctcgc gttctgcctg
      901 ctctttctgt tccacggggc cgggttcctc tccctgagga ccgtcggcga cctcggccag
      961 cggtcggcca ggatggcacg ccggctggcg gcgccggccc tgctcgccgg aatccttttc
     1021 atggtttggt gcgtggccac cgcggtggcg gaaaacgacc gcggcgtcct cggtccgctc
     1081 gtcccggcgg tggccggatc actcgctctc tgcggcgcgg cccttgccgc ccgtgcccga
     1141 cgccccggtc tctccttcac tctgggagcg gccggcatcg tcggtgtcat cgccaccctc
     1201 ttcaccgggc tctacccgcg ggtgatggtt tcctcgaccg acctcgccaa cagcctcacc
     1261 accggcaacg ccgcctcttc ccattactcg ctgacggtga tcacgatcgt cgccggatgc
     1321 cttttgccgg tcgtgctcgc ctaccagtcc tggagctact gggtgttccg ccaccgggtt
     1381 tccgaggatg gcttcgagca ggccggaaac ccgatcgagg cgatcaggca gcggaccgaa
     1441 aagcgaaggt aatcccctcg cccgatccgg ggaccacagg ttttggctag gctcaacctg
     1501 tgagtccagc cgccgccagg acaaccggag gaaaacccga ttcgctgccg accgccgcgg
     1561 tcgaggacta cgcgaaggcg atctactcac tgaccggctg gggtgaggaa accgcttcca
     1621 ccaacgacct cgccgagcgg ctcggagtga aggccggctc ggtctcggcg atgatcaaga
     1681 agctggatga agcggggctg gtcgagcggg ttccgtacca cggcgtcagg ctgaccgccg
     1741 acggcacgcg ggtggcaatg gcggtgcttc gccggcaccg gctgctcgaa ctattcctcg
     1801 ccgaggttct ggacgtgccc tgggaccggg ttcacgccga ggcggaggtt ctcgaacacg
     1861 ctctgtccga ggatctgacc gacctgatct cgaccaagct gggcgacccc gacttcgatc
     1921 ctcacggcga cccgatcccg gaccgggatc tggcgatcgt cgaaacagag acgaccgccc
     1981 tggccgacct cgcgcccggt gattcggccc ggttcgtacg ggtttcggac tccaacccgg
     2041 agatgcttgc ctacctcagc cggtgcggaa tcgcggtcgg tgaccagctg gagttgatcg
     2101 aacggcaacc ctttgacgga ccgatcacgg tccgcatcga cagcgactcg cacgtcctcg
     2161 gcctgacc
//
LOCUS       DAOQMF010000314         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_33520_length_2161_cov_2.546534, whole genome shotgun sequence.
ACCESSION   DAOQMF010000314 DAOQMF010000000
VERSION     DAOQMF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33520_length_2161_cov_2.546534"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="PLV77_05970"
     CDS             <1..1804
                     /locus_tag="PLV77_05970"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15427.1"
                     /translation="LDSGGSAFSTLLGVYTGTTVNNLKEVGSAATKPAGRKAYVQFDA
                     KKGTNYFIAVASSSTNTAGQIQLLLAPGGTPDTTPPVVQVASPVSGLIINSNTIDIVA
                     TAFDPGPISSGVRKLAFQVKPVTAGSSIAPTGSTQTTNRVALVEGRNSVSVTAVDGVG
                     NVSDPVTVVVIYRRQPVANDHFAYATGLNGVAGAVVSDNREATREPGEPKHAGNDGGH
                     SLWWKWTAPADGSLLLSTDGSKLDTLLGMYTGSRVDSLHTIASNDDAFDGSKFSKLQQ
                     GVRANQTYYLAVDGFNGATGQVQLAYNFTPGALYTINLQTGTGGSVSEHGAGPVDVPA
                     NSTQTLTAVPDPGYVFAGWEGAAVTTVNPLSLQVNSNITVTANFYPVAFTDDFETGGL
                     GRLNWVTTGTPGWSVTSATAAGGKYSARSGAIGNSQASTLSLAGKFSAGSVAFSLKIS
                     SEAVWDYGEFLIDGVQLFKWSGEADWTEYSFPIAEGVHTLQWIYHKDAQTSAGLDAVY
                     LDNLQAPLVPAAGPGSAANLSLTLLPQGQYELKLVGQTNQVYIIQQAVEILPNQQTVW
                     TPYSTNVAEYGQVRFILDPQALNRPRTFFRAVTK"
BASE COUNT          411 a          638 c          692 g          420 t
ORIGIN      
        1 gctggatagc ggcggcagtg ccttcagtac tttgctcggc gtctacacgg ggacgaccgt
       61 caataacctc aaggaagttg gctcggcggc gaccaagccg gccggccgga aggcctacgt
      121 gcagttcgac gcgaagaagg ggacgaacta cttcatcgcg gtggcgagca gcagcaccaa
      181 cacggcgggg cagattcagt tgctgcttgc gcccggaggt acgccggaca cgacgccgcc
      241 ggtcgttcaa gttgccagcc cggtgagcgg gttgattatc aacagcaaca cgatcgatat
      301 cgtcgccacg gcgttcgatc ccggtccgat ctcaagtgga gtccggaaac tggccttcca
      361 ggtcaagccg gtgactgcgg gaagctcgat tgccccgact ggaagcacgc agaccacgaa
      421 tcgcgtcgcg ttggtggaag gacggaattc ggtctcggtg actgccgttg atggtgtggg
      481 taacgtctcg gaccccgtga ccgtggtggt gatctaccgc cgccagccgg tggccaacga
      541 ccatttcgct tatgcgaccg gtttgaacgg cgtcgccggg gcggtggtct ccgacaatcg
      601 cgaagccacc cgcgaaccgg gcgaacccaa gcacgccggt aacgacggcg ggcactcgct
      661 gtggtggaaa tggactgcgc cggccgacgg atcgctcctg ctcagcacgg atgggagtaa
      721 gctggatacc ctcctgggca tgtacaccgg aagccgagtg gacagtctcc atacgatcgc
      781 ttcgaacgac gatgctttcg acggttcgaa gttcagcaag ctgcagcagg gagtccgcgc
      841 caatcagacc tattacctcg cggtggatgg tttcaacggg gcgacggggc aagtgcaact
      901 cgcttacaac ttcactccgg gagcgttgta cacgatcaac ctccagaccg ggaccggagg
      961 ctccgtatcc gagcacggtg ccgggccggt cgatgtgccg gcgaattcga cgcagacact
     1021 cacggcagtt cccgatcccg gctatgtctt cgcgggctgg gagggggcgg ccgtgaccac
     1081 ggtgaatccg ctgtcgttgc aggttaactc gaacatcacg gtaacggcca acttctaccc
     1141 ggtggcattc acggatgact tcgaaaccgg tggactcggg cggctgaact gggtgacgac
     1201 cggaacgccg ggttggtccg tgaccagtgc cacagcggct ggcggcaagt actcggcccg
     1261 ctccggcgcc attggtaaca gtcaggcgtc gaccctgtcg ctcgcaggaa aattcagcgc
     1321 cggttcggtg gctttcagtc tcaagatcag ctccgaggca gtttgggatt acggcgaatt
     1381 cctgattgat ggagtgcagc tcttcaagtg gtcgggtgag gcggattgga cggagtacag
     1441 cttcccgatc gccgaaggtg ttcatacctt gcagtggatt taccacaagg acgcccagac
     1501 ctcggccgga ttggatgccg tttacctgga caacctgcag gccccgttgg tgcccgcggc
     1561 gggtccgggg agcgcggcca atctctcgct tactttgctg ccgcagggac agtacgagct
     1621 caaattggtg gggcagacca atcaggtcta catcatccag caggcggtgg agattctgcc
     1681 gaaccagcag accgtctgga ctccgtattc cacgaacgtt gcggagtacg gccaggtccg
     1741 gttcattctg gatccgcaag ccctgaaccg tccgcgtaca ttcttccggg cggtcaccaa
     1801 gtagcggacc ggacgttgtg aattcgccgg ctgcgggccc cgtgcctgca gccggttttc
     1861 tatttcgatt tccgacactg aatgggaatc cacgcggttt ggattaggct cgcctccgaa
     1921 tcgggtgcgg aagagagtcc ccgaccgatc cgaagccagc ctggtcccaa aaccgaactt
     1981 gggcccgtgc ccgccgtgat ggttgtcctt gtgtgctttc agacatatgc agcccggaag
     2041 ttctgctctc atgcggctcg gtacgccgtt cgatttcgag gcatcgctac cgggggcggg
     2101 tttggtcgta accggaacga atcgtgctta ttccgtgcga ctgcattgaa tgcccctatc
     2161 a
//
LOCUS       DAOQMF010000315         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_33550_length_2160_cov_2.695012, whole genome shotgun sequence.
ACCESSION   DAOQMF010000315 DAOQMF010000000
VERSION     DAOQMF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33550_length_2160_cov_2.695012"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..669)
                     /locus_tag="PLV77_05975"
     CDS             complement(226..669)
                     /locus_tag="PLV77_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNC15428.1"
                     /translation="MKPAEHMTGECMCGKVKFEVDQPLIGAALCYCKRCQRRTGSGVS
                     ATGLTQPGSFRITEGEDSVTSYEPGDGGFNKYFCRNCSGQLFTSHPDNPEILAVRLGA
                     LNEDPGIRPAAHQFTAYAAPWFPVPDDGLPKFPERLDWTQVLGQQ"
     gene            838..>2160
                     /locus_tag="PLV77_05980"
     CDS             838..>2160
                     /locus_tag="PLV77_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC15429.1"
                     /translation="MQVSSSGAMRPLGRLFGVLFGLGAVLMLLFAMPLAGRADAAKKP
                     PRVTIRTTQYGIPRILADNPYGLGYGYGWAIASEQLCTLADTYTTVRGERSLYFGGDV
                     NAPNGVTNLDSDFFWKRVRKEGTVQKMINLKPPNGPLPDVKQAVRGYAAGYNAYLRKT
                     GVGKLPDPRCRGKAWVKPISVLDVYMRFYQLLGYGSTDPSMAGIAEAQPPLPMTRKNG
                     SAPADEVNPADITTDDFPKGFGNFTGGLGSNAVALGKNSVKGGGGLLLGNPHFPWHGA
                     QRFFESQLTIPGRLNVSGASLLGVPVILIGHTRTMAWSHTVSTARRFILFREALDPTD
                     PTRYIVDGQSKPMKATTVRVKDKDGMVHTRTLYSTEHGYMTNQLQGSNLFGWATDYGY
                     SLYDANTGNFRMINHFYSMNQARSVPEVLKILKKYQGIPWVNTIASDSK"
BASE COUNT          396 a          704 c          694 g          366 t
ORIGIN      
        1 aaggcggccg gaccagactg gtgaccctca ctacattcga ggctagggag taccgcgacg
       61 gcatgcttca gtcgggcatg gagaagggca tggacgaggg ctacgcgaag ctcgatcgac
      121 tgctcgccga ccgaacctga ctgcagtcgg tccggactgc gccggacctc ccatggaaac
      181 cggcccctcc cacccgaagg aagtaatcgc tgggaggggc tctgcttact gttgcccgag
      241 aacctgggtc cagtcgagtc tttcggggaa tttgggcagg ccgtcatccg gcaccgggaa
      301 ccagggcgcg gcataggcgg tgaattggtg ggcagcgggg cggatgcccg ggtcctcgtt
      361 gagggcgcca agccggaccg cgaggatctc cgggttgtcg ggatgcgagg tgaaaagctg
      421 gccgctgcag ttgcggcaga agtacttgtt gaagccgccg tcgccgggct cgtaactcgt
      481 gacggaatcc tcgccttcag tgatccggaa cgagcccggc tgggtcagtc cggtggccga
      541 gacgcccgat ccggtgcggc gctggcagcg tttgcagtag cagagggccg ccccgatcag
      601 gggctggtcg acctcgaact tcaccttgcc gcacatgcac tcgccggtca tgtgttcggc
      661 cggtttcaaa gtttgatcct gcgctgagct ctccatgatc caactctagt ccgaacggag
      721 ccgcccccag ccattcaggg cttcaaacgg gagggcgggc tctcgccggg gtccgcacag
      781 accggaccga ctgcagttag tcttttcgcg agggaaacaa ccgaaggaga gtcaatgatg
      841 caggtgtctt cgtccggggc aatgcgtccc ctgggccgtt tgttcggggt tctgttcggg
      901 cttggcgcgg tactgatgct ccttttcgcc atgccgctgg cgggccgggc cgacgcggcg
      961 aagaagccgc cgcgggtcac gatccgcacc acccagtacg ggatcccgcg gatcctcgcc
     1021 gacaacccct acgggctcgg ctacggctac ggctgggcga tcgccagcga gcagctatgc
     1081 acgctagctg acacctacac cacggttcgg ggcgagcggt cgctctactt cggcggcgac
     1141 gtcaacgccc cgaatggcgt tacgaacctc gactcggatt tcttctggaa gcgggtgagg
     1201 aaggagggca ccgtccagaa gatgatcaac ctgaagccgc cgaacgggcc gctgcccgac
     1261 gtcaagcagg cggtcagggg atacgccgcc ggttacaacg cctacctcag gaagaccggg
     1321 gtagggaagc tgcccgaccc gcgttgccgc ggcaaggcct gggtcaagcc gatctcggtg
     1381 ctcgacgtct acatgcgctt ctaccaactg ctcggttacg gctcgaccga cccctcgatg
     1441 gccggcatcg ccgaggccca gccgccgttg ccgatgacga ggaagaacgg ctccgccccg
     1501 gccgatgaag tcaatcccgc cgacatcact accgatgatt tcccgaaggg cttcggcaac
     1561 ttcaccggcg gtctcggctc gaacgcggtc gccctgggca agaactcggt caagggcggc
     1621 ggcggccttc tgctcggcaa cccgcacttc ccctggcatg gtgcccagcg cttcttcgag
     1681 tcgcagctga ccatccccgg acggctcaac gtttccgggg ccagcctgct cggggtgccg
     1741 gtgatcctga tcggccacac ccgcaccatg gcctggagcc acacggtttc gaccgcccgc
     1801 cgtttcatcc tcttccggga ggcgctcgac ccgaccgacc cgacccgcta catcgttgac
     1861 ggccagtcga agccgatgaa ggcgaccacg gtcagggtca aggacaagga cgggatggtt
     1921 cacaccagga ccctctattc gaccgagcac ggctacatga ccaaccagct ccagggcagc
     1981 aacctgttcg gctgggcgac ggactacggc tactcgctct atgacgcgaa caccggcaac
     2041 ttccggatga tcaaccactt ctactcgatg aatcaggccc ggagcgtgcc cgaggtcctg
     2101 aagatcctga agaagtacca gggcatccct tgggtcaaca cgatcgcttc cgattcgaag
//
LOCUS       DAOQMF010000316         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_33584_length_2159_cov_2.982890, whole genome shotgun sequence.
ACCESSION   DAOQMF010000316 DAOQMF010000000
VERSION     DAOQMF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33584_length_2159_cov_2.982890"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLV77_05985"
     CDS             <1..189
                     /locus_tag="PLV77_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15430.1"
                     /translation="TFTLVWYLTLANFSSGRIKLPERLRPLLGFATKYHAVLLTGAVL
                     IVVALIAEAFWSYWSTLP"
     gene            218..682
                     /locus_tag="PLV77_05990"
     CDS             218..682
                     /locus_tag="PLV77_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15431.1"
                     /translation="MNPKTTRMLAAGAVVVVALALLLIVRPGDSDNGNPDENADQGQA
                     TSKTTGNTGPTSAPQSKPPQPEKPVVPVYRVVDGQPAGGVQKLEANEGDNIRFVVKSD
                     VDDEVHVHGFDVSEPVGPGKPAKFDFKAGFTGVFEIELENSAVPIIELQVNP"
     gene            679..2067
                     /locus_tag="PLV77_05995"
     CDS             679..2067
                     /locus_tag="PLV77_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HNC15432.1"
                     /translation="MISLPLAHALVSRGDLPIPVWLFAWGAGVVLVVSFFALASAWRK
                     PRFEDAPGRPAATWLSATANSSAVQFLSGLIGVLLLAVVLYAGIEGTEAADRNFSLTF
                     VFVTVWLGFPVLSVVFGNVFTAFSPWRAIGRAYGWLAQRLAPGTVRHVEYPEKFGRWP
                     AAVGLVAFVWLEIIYGATGAAVVLSPEIVAKATVIYSVYTLVMMGVFGVEKWSSRGEF
                     FSVYFGMFASMSSFRKREGKLERRRWLSGTTDWANVPGSLALVVSAIGVTTFDGAQEG
                     ALKTPIEWTQERIGDLGFAATVSVRAGDTIFLLITIGIVALIYLLGVKGMTRVDAAPP
                     FKVLRSRFAHTLIPIALAYLVAHYFSLFVFQEQAQFTFLLSDPLGDGSNLFGTATSGI
                     DYTLIGPELVWYVQVGALIAGHVTALVLAHDRALLIWPDYRLATRSQYWMLAVMLCFT
                     SLGLFLLSFANQ"
BASE COUNT          385 a          698 c          619 g          457 t
ORIGIN      
        1 accttcaccc tcgtctggta cctgacgctg gccaacttct cgagtggacg aatcaagttg
       61 ccagagcgcc ttcgtcccct ccttggattc gcgacgaaat accacgccgt actactcacg
      121 ggagcggtcc tgatcgtcgt cgccctgatc gccgaagcct tctggtcgta ctggagcacc
      181 ttgccgtgac ccccaacgaa acgagttgaa gacccacatg aatccgaaga ccacccgcat
      241 gctcgccgcc ggcgccgttg tcgtcgttgc gctcgcgctt ctacttatcg tcaggcccgg
      301 cgactccgac aacggcaacc ctgacgagaa cgccgaccag ggacaggcga catccaagac
      361 caccgggaac accgggccca cttcggcccc gcaaagcaag ccgccccagc ctgagaagcc
      421 ggtcgttccc gtttaccggg ttgtcgacgg acagcccgcg ggcggagttc agaagctcga
      481 ggccaatgag ggcgacaaca ttcgattcgt ggtcaagtcc gatgttgatg acgaggtcca
      541 cgtccacgga ttcgatgtca gcgaacccgt aggtcccggc aaaccggcca agtttgactt
      601 caaagccggc ttcaccggag tcttcgaaat cgaactcgag aactcagccg tcccgatcat
      661 cgagctccag gtcaaccctt gatcagtctg cccctcgcgc atgccctggt ctcgcggggt
      721 gacctgccga ttccggtttg gcttttcgcg tggggcgccg gcgtagtcct tgtcgtctca
      781 tttttcgcgc tggcctcggc ctggcgcaaa cccaggttcg aagatgctcc gggtcgaccc
      841 gcagcgacct ggctttcagc gacagcaaac tcttcggccg ttcagttcct ctcaggtctg
      901 atcggcgtcc ttctacttgc ggttgtcctc tacgcgggga tcgaaggaac cgaagccgcc
      961 gaccgaaact tctctctgac cttcgtcttc gtaaccgtct ggctcggctt ccccgtcctg
     1021 agtgtggtct tcgggaacgt cttcaccgcg ttctccccct ggagggcaat tggcagagct
     1081 tacggatggc ttgcgcagag actggcgccg ggaaccgtaa ggcacgtcga gtaccccgag
     1141 aagttcggca ggtggccggc ggcggtcgga cttgtcgcct tcgtctggct cgagatcatc
     1201 tacggagcga cgggcgcggc cgtggttctc tcccccgaaa tcgtggccaa ggcgacggtc
     1261 atctactcgg tctacaccct ggtgatgatg ggcgtgttcg gcgttgagaa atggagctca
     1321 cgtggagagt tcttctcggt ctacttcggc atgttcgcct cgatgtcgtc gtttcgaaag
     1381 cgggaaggaa agctcgagcg tcgacgttgg ctctcgggaa ccaccgattg ggcaaacgtg
     1441 ccgggatcgc tggcgctcgt cgtttctgcc atcggtgtga ccaccttcga cggtgcgcag
     1501 gaaggtgcgc tgaaaacccc gatcgagtgg acgcaggaac gaatcggtga cctggggttc
     1561 gcggcaacag tctcggtccg ggctggcgac accatcttcc tgctgatcac gatcggaatc
     1621 gtggcactca tctacctcct cggagtcaag ggaatgaccc gggtggatgc ggcgccaccg
     1681 ttcaaggtgc ttcgtagccg ctttgctcac acactcattc cgatcgcgct ggcatacctc
     1741 gtggcccact acttcagtct cttcgtgttc caggagcaag cccagttcac ctttctgctc
     1801 tcagatccgc tgggcgacgg atcgaatctg ttcgggacag cgacatcagg gatcgactac
     1861 accctgatcg ggcccgaact tgtctggtac gtccaggtcg gggcactcat cgccggccac
     1921 gtgactgcgc tcgtcctcgc tcatgaccgc gcacttctga tctggccgga ctaccgactg
     1981 gcgaccagat cccagtactg gatgctcgcg gtcatgctct gctttacgag tctgggcctc
     2041 ttcctgctct catttgcgaa ccagtagaac gggcgcttca ttgcagggct acacctcgcc
     2101 ggctcaaccc aagatctaac tactacagtc cgtaggagct tggctcccct tccctcgaa
//
LOCUS       DAOQMF010000317         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_33631_length_2157_cov_3.637964, whole genome shotgun sequence.
ACCESSION   DAOQMF010000317 DAOQMF010000000
VERSION     DAOQMF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33631_length_2157_cov_3.637964"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLV77_06000"
     CDS             complement(<1..279)
                     /locus_tag="PLV77_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15433.1"
                     /translation="MTESVETSSSRRARQRAMMIAVDTDGQGTLDELGELLRTAGVAT
                     VGEVIQRRSEPDPDRYFGRGKLEEVKAEIKRTGANLVACDDELAPRQER"
     gene            353..1351
                     /locus_tag="PLV77_06005"
     CDS             353..1351
                     /locus_tag="PLV77_06005"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC15434.1"
                     /translation="MKIFMTGATGFIGGATARHLVEQGHQLTCLVRDRSKAGSLGELG
                     CELVDGDLSSRPRLAEQMKGHDALLHNAALYEVGIPKDRIPALRKANVEGTVNTLEAA
                     LEAGIPRVLYVSTCAVFGNTHGEIATEDFRRPDLDSPGGLEFTSVYEETKYEAHQIAR
                     NLIETRNLPCVIAQPGGVYGPNDHSELGSTIHQFLDGKLPMVPFPEFGTGLTHVEDIA
                     AGLALILDKGEIGESYILNEGNFTMRQILEEVGGIADRKVPKRAMPTVVLKALRPVGP
                     LVGKIMGQPPNLAELIASTDGVTFWADGTKAKTELGWHPREVRTGLRQTLEAEGRL"
     gene            complement(1348..>2157)
                     /locus_tag="PLV77_06010"
     CDS             complement(1348..>2157)
                     /locus_tag="PLV77_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC15435.1"
                     /translation="VALASDPGYPVYTGGPILAGAEAVLLPLKESLGFAPDLEAIDAR
                     TAERARLLFLNYPNNPSGAIVPDGFFEQVVDFARENEILVVHDNAYSETTYDGYVAPS
                     FLATPGAKEVGVEVFSLSKGYNMTGWRVGAILGNPDAISTYWRLKTNVDSGLFEAIQL
                     AAVAALDGPSQPLEEMNAIYARRRDLVVDTLRAIGVDVKSPKGTIYVWAPVPKGFTST
                     SFCEKVLEEAAVVISPGSMYGRSGEGYFRISLTTPDERLSEALERMREHLA"
BASE COUNT          380 a          730 c          691 g          356 t
ORIGIN      
        1 ccgttcctgc cggggagcca gctcgtcgtc acaggcgacg aggttcgcgc cggtgcgctt
       61 gatttccgcc ttgacctctt ccagcttgcc gcgaccgaag taacggtccg gatccggttc
      121 gctgcgacgc tggatcacct cgccaaccgt ggcgacgccg gcggtccgga gcagctcgcc
      181 gagttcgtcc agggtcccct gcccgtccgt atcgacggcg atcatcatcg cccgctgccg
      241 ggccctgcga ctgcttgagg tttcaactga ctcggtcact gacttcatcc tagaactcgc
      301 aggacggcgg ccaactccgg gccgcccgcg ggctcgcgct acgctcccct ccatgaagat
      361 cttcatgacg ggagcgaccg gtttcatcgg cggtgcgacg gccaggcacc tggtcgagca
      421 gggccatcag ctgacctgcc tggtccggga caggtcgaag gcaggcagcc tgggtgaact
      481 cggttgcgag ctggttgacg gtgacctttc gtcgcggccc cggctggccg agcagatgaa
      541 gggccatgac gccctgctcc acaacgccgc gctctatgag gtcggcatcc ccaaggatcg
      601 catcccggca cttcgcaagg caaacgtcga aggcaccgtc aacaccctgg aagcggccct
      661 cgaagccggc atcccccggg tcctgtacgt ctcgacctgc gccgtcttcg gcaacacgca
      721 cggcgaaatc gcgaccgagg acttccgccg gcccgatctc gacagtcccg gcggcctcga
      781 gttcacctcg gtttacgagg aaaccaagta cgaggctcac cagatcgcca ggaacctgat
      841 cgagacccgg aacctgccct gcgtgatcgc ccagcccggc ggggtctacg ggccgaatga
      901 ccactcggag ctcggctcga cgatccatca gttcctcgac ggcaagctgc cgatggttcc
      961 cttccccgag ttcggcaccg gcctgaccca tgtcgaggac atcgcggccg gcctggccct
     1021 gatcctcgac aagggagaga tcggcgagag ttacatcctc aacgaaggca acttcacgat
     1081 gcgccagatc ctcgaggagg ttggcgggat cgccgaccgc aaggttccca agcgggcgat
     1141 gccgaccgtc gttctcaagg ctttgagacc ggtcggaccc ctggtcggca agatcatggg
     1201 ccagccgccc aatctggccg agctgatcgc ctcaaccgac ggcgtcacct tctgggccga
     1261 cgggaccaag gccaagaccg aactgggctg gcacccgaga gaggtcagaa ccggcctccg
     1321 ccagaccctc gaagccgaag gaagactcta ggcgaggtgc tcgcgcatcc gctcgagggc
     1381 ctcgctgagg cgctcgtcgg gtgtggtcag cgagatgcgg aagtaccctt cgccgcttcg
     1441 cccgtacatc gaaccgggtg agatcacgac cgctgcctct tccagaacct tctcgcagaa
     1501 gctggtcgag gtgaagccct tcggcaccgg ggcccagacg tagatcgtgc ccttggggct
     1561 cttcacgtcc acgccgatcg ccctcagggt gtcaaccacc aggtcgcggc gtcgcgcgta
     1621 gatcgcgttc atctcttcca gcggctggga agggccgtcg agggccgcca ccgcggccag
     1681 ctggatcgcc tcgaaaagac cggaatcgac gttggtcttg agccgccagt aggtcgagat
     1741 cgcatccggg ttgccgagga tcgccccgac ccgccagccg gtcatgttgt agcccttcga
     1801 aagcgagaag acctcgactc cgacttcctt cgccccgggg gtcgcgagga agctcggggc
     1861 gacatatccg tcgtaggtcg tctccgagta ggcgttgtcg tgaaccacga ggatctcgtt
     1921 ctcgcgggcg aagtcgacga cctgttcgaa gaagccgtcg ggaacgatcg cgccagaggg
     1981 gttgttcgga tagttgagga agagcagccg ggcccgctcg gcggtccggg cgtcgatcgc
     2041 ttcgagatca ggggcaaaac ccaggctttc cttgagcggc agcagcaccg cctcggcgcc
     2101 ggcgaggatc ggaccccccg tgtagaccgg gtatcccggg tcggaagcga gggcaac
//
LOCUS       DAOQMF010000318         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_33634_length_2157_cov_3.451951, whole genome shotgun sequence.
ACCESSION   DAOQMF010000318 DAOQMF010000000
VERSION     DAOQMF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33634_length_2157_cov_3.451951"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1585
                     /locus_tag="PLV77_06015"
     CDS             278..1585
                     /locus_tag="PLV77_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC15436.1"
                     /translation="MTLGALVVAIVFVASLIFKGDSGYKYSFDFQSASGMVSGNFVMI
                     GGSPVGKVTKVELTEDNRVKMEVEIDRELHEGTSAVIRKSSLSSLHNHYISLQPGPDN
                     EPVLPEGHDFGEGVTQTAVELDQFFDTFDETTRKGWSNWIQGIAAIYAGEGAEGANKT
                     FKYSGAAFSSTQRLMAELADQDTRLDQFVGNTSKFVTQLAEVSPQLTELVSNSNIALG
                     KIAENNESLSLALQELPPTLRQGNTTFANLRAALDDVQPMIAASGRAADAGLANYLKN
                     DLRPVLIRARPVFNDLALAAGRPGPDNDTSDLLSQLIPLHGRAEPAVNAVVRALNANQ
                     EEVSEMRAYAPDIFGSFNKLAGLLGSYDANGPFAKVTPTATGVFQLNGTAIQPADTAI
                     YGGLDVITGINRCPGGGVQAIAGSNPFLDNGNLTGKCDPSQVP"
     gene            1585..>2157
                     /locus_tag="PLV77_06020"
     CDS             1585..>2157
                     /locus_tag="PLV77_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC15437.1"
                     /translation="MLKRIITILLVILAIVAVVLLVRSCNDDSDAYEVRAVFDNGAFV
                     VPGIDVRVAGANVGSVSSVDVSRPGEETGTDVPGKAIVVMNITDEGFQDWKDDASCII
                     RPQSLIGEKFLDCQVTQPRPAGSEPPPSLDQIPDGQPGAGEYLLPIQNNAQSVDLDLI
                     NNIYRLPYAQRFRIILNELGAGLATRGAKVS"
BASE COUNT          413 a          727 c          643 g          374 t
ORIGIN      
        1 gaattgccgc gggtttccga aaccgcggtc gagatgatcg acgggttcct gcgggaaggt
       61 ggaagcgtca ccaatccctg tgcgccgggt ccctgcctct ccggcggctg gaccggctct
      121 tgacgtcagg ctgtaacgcc gggcgctcag gtagcatgcg cgcaggaagg aggcttcttt
      181 ggtggggaga atcaccgggt agctcagatt gacgactgcc aatcaagcaa atccaagtcg
      241 cgtcaatcgc ggacgtagct cgaccgtgct cagagttgtg actctgggcg cgcttgtagt
      301 tgcgatcgtt ttcgtcgctt ccctcatttt caagggagat tcaggctaca agtacagctt
      361 cgatttccag agcgcgagcg gcatggtctc gggcaacttc gtgatgatcg gcggctcgcc
      421 cgtgggcaag gtcaccaagg tcgagttgac cgaggacaac cgggtgaaga tggaggtcga
      481 gatcgaccgc gagcttcacg aaggcacctc ggcggtcatc cgcaagagtt ccctttcctc
      541 gctgcataac cactacatct cgctgcagcc cggcccggac aatgaaccag tactccccga
      601 aggccatgat ttcggcgaag gcgtaaccca gacggcggtc gagctcgacc agttcttcga
      661 cacctttgac gagaccaccc gcaaaggctg gtcgaactgg atccagggca tagccgcgat
      721 ctacgccggc gaaggggccg aaggcgcaaa caagaccttc aagtacagcg gggccgcctt
      781 ctcaagcacc cagcggctga tggctgaact ggccgaccag gacaccaggc tcgatcagtt
      841 cgtcggaaac acctcgaagt tcgtcaccca gctggccgag gtctcgccgc agctgaccga
      901 actggtctcc aactcgaaca ttgccctcgg caagatcgcc gagaacaacg agtcgctctc
      961 gctggcactt caggagctgc cgccgaccct gcgtcagggc aacaccacct tcgccaatct
     1021 gcgggcggcg ctcgacgatg tccagccgat gatcgccgcc tccggtcgtg ccgccgatgc
     1081 cgggctggcc aactacctga agaacgacct tcgtccggtc ctgatccggg cccgtccggt
     1141 cttcaacgac ctcgccctgg cggctggtcg tcctggcccg gacaacgaca cttccgacct
     1201 gctctcgcag ctgatcccgc tgcatggccg ggccgaaccc gcggtcaacg ccgtggtccg
     1261 ggccctgaac gccaaccagg aagaagtttc cgagatgcgg gcctacgcgc ctgacatctt
     1321 cggttccttc aacaagctgg ccggtttgct cggcagctac gacgcgaacg gtcctttcgc
     1381 caaggtaact ccgaccgcga ccggcgtgtt ccagctcaac ggcaccgcga tccagccggc
     1441 cgacaccgcg atctacggcg gcctcgacgt aatcaccggc atcaaccgct gcccgggtgg
     1501 cggcgtccag gccatcgccg gctcgaaccc cttcctcgac aacggcaacc tgaccggcaa
     1561 atgcgacccc tcacaggtac cgtgatgctg aagcgaatca tcacaatcct gctcgtgatc
     1621 ctggcgatcg ttgccgtcgt cctgctggta cgcagctgca atgacgacag cgatgcctat
     1681 gaggtccggg cggtcttcga caacggcgcc ttcgtggtac ccgggatcga cgtccgcgtg
     1741 gccggtgcca acgtcggcag cgtcagctcg gtcgatgttt cgcggcccgg cgaagagacg
     1801 ggtacggacg tgcccggcaa ggcgatcgtg gtgatgaaca tcaccgacga gggcttccag
     1861 gactggaagg acgatgcctc ctgcatcatc cggccgcagt cgctgatcgg cgagaaattc
     1921 ctcgactgcc aggtgaccca gccccgtccc gccggcagcg agccaccacc ctcgctcgac
     1981 cagatccccg acggtcagcc cggtgccggc gaatatctgc tgccgatcca gaacaacgcc
     2041 cagtcggttg acctcgacct gatcaacaac atctaccgcc tgccgtacgc gcagcgtttc
     2101 cggatcatcc tcaacgaact gggagccggt ctggcgaccc gcggcgccaa ggtctcc
//
LOCUS       DAOQMF010000319         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_33672_length_2156_cov_4.046644, whole genome shotgun sequence.
ACCESSION   DAOQMF010000319 DAOQMF010000000
VERSION     DAOQMF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33672_length_2156_cov_4.046644"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="PLV77_06025"
     CDS             complement(<1..1648)
                     /locus_tag="PLV77_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNC15438.1"
                     /translation="MSSTYTPTNRSRTLADLLPNSAARHGSDGAFLFKNEAGSWEETS
                     YEKVAAEVQALTLGLKALGVQRGDKVAIIGNTRPEWTLFDFAAMSTGATVVPIYQSNS
                     PSECQYVLEHSESRVVIAENEDQLAKIREVRDSLPALENVVIMIGEADDAISMNELRA
                     KGDSGSAEDYKASYEAVEPDDLCKIVYTSGTTGPPKGCMISHGNYRAMLDMADSASIV
                     EEGETSYLFLPLAHVFALLIQYGMIDIGGRIAFWERDQLKIVPNLAEVKPESFPSVPR
                     IFEKVHDTAVATAEGEGGLKAAIFRWAFRVGKRYRKAEAQGKKPGFLLTKQFNFADKA
                     VFSKIREIFGGNLRVAISGAAPIDPEILRFYEAAGVPLFEAWGMTETSTGGTTNMPGE
                     NRIGTVGKPVDGVELRIAEDGELLIKGDNVFQGYYKNPEATAETITDGWLHTGDIASI
                     DEDGYVSIIGRKKEIIITAGGKNITPVNIEQEVKRHPLVSQCVVIGDRRPYLIALITL
                     DQEALTRFAAEQGMLDEPGAMVGSPLVRETLEQHIEEVNRN"
     gene            complement(1710..>2156)
                     /locus_tag="PLV77_06030"
     CDS             complement(1710..>2156)
                     /locus_tag="PLV77_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNC15439.1"
                     /translation="EIGNNALIVRQLGFEPVPGEIGEAKGRHIGQIGRRAEPIPATAL
                     FELVGRELGREPLVFAEGPEMVSTIGVVTGAGASDIHEAIALGLDAYLTGEPSEHVMA
                     DAREGAIHFIAAGHYATEICGIAALGDLVAQRFEVEHEFLDLPNPI"
BASE COUNT          340 a          676 c          708 g          432 t
ORIGIN      
        1 agttcctgtt gacttcctcg atgtgctgct cgagcgtctc gcggaccagt gggctgccga
       61 ccatcgcacc cggctcgtcc agcatgccct gctcggcggc gaagcgggtc agcgcttcct
      121 ggtcgagggt gatcagggcg atcaggtacg gccgccggtc accgatcacc acgcactggg
      181 aaacgagcgg atgccgcttg acttcctgct cgatgttgac cggggtgatg ttcttgccgc
      241 cggcggtgat gatgatctcc ttcttgcggc cgatgatcga gacgtagccg tcctcgtcga
      301 ttgaggcgat gtcaccggtg tggagccagc cgtcggtgat cgtctcggcc gtcgcctcgg
      361 ggttcttgta atacccctgg aagacgttgt cgcccttgat cagcagctcg ccatcctcgg
      421 cgatcctgag ttcgaccccg tcgaccggct tgccgaccgt gccgatccgg ttttcgcccg
      481 gcatgttggt ggtgccaccg gtcgaggtct cggtcatgcc ccaggcctcg aacaagggca
      541 ctccggccgc ttcgtagaag cggaggatct ccggatcgat cggcgcggcg ccgctgatcg
      601 cgacccgcag gttgccgccg aagatctccc gaatcttgga gaagaccgcc ttgtcggcga
      661 aattgaactg cttggtcagc aggaagccgg gctttttgcc ctgggcctcg gccttgcggt
      721 aacgcttgcc gacccggaaa gcccagcgaa agatggccgc cttgaggcca ccctcgccct
      781 cggccgtggc gaccgcggtg tcgtggacct tctcgaaaat gcgggggacc gagggaaagg
      841 actcgggctt gacctcggcc aggttgggga cgattttcag ctggtcacgc tcccagaagg
      901 cgatccggcc gccgatgtcg atcatcccgt actggatcag cagggcgaag acgtgcgcga
      961 gcggcaggaa cagatagctg gtctcgcctt cctcgacgat gcttgccgag tcagccatgt
     1021 ccagcatcgc ccggtaattg ccgtgggaga tcatgcagcc cttgggcggc ccggtcgtgc
     1081 cggaggtgta gacaatcttg cagaggtcgt cgggttcgac cgcctcgtag ctcgccttgt
     1141 agtcctcggc gctgccggaa tcacccttcg cccgcagctc gttcatgctg atcgcgtcgt
     1201 cggcttcgcc gatcatgatc acgacgtttt caagggccgg cagtgaatcc ctgacctcgc
     1261 ggatcttggc cagttgatcc tcgttttcgg cgatcacgac ccgggactct gaatgctcga
     1321 gcacgtactg gcactcggag ggcgagttgc tctggtagat cggcaccacg gtcgcaccgg
     1381 tgctcatcgc ggcgaagtcg aagagggtcc attcgggcct ggtgttgccg atgatcgcga
     1441 ccttgtcgcc acgctggacg ccgagggcct tcagaccgag ggtgagcgcc tggacctccg
     1501 ccgccacctt ttcgtaggag gtctcctccc agctgccggc ctcgttcttg aagagaaagg
     1561 caccgtccga tccgtgcctg gcagccgagt tcggcagcag gtcagcgagg gttcgggaac
     1621 ggttggtcgg cgtgtaggtg ctggacaagt ctctctccta tgtggctatg tggcgacgca
     1681 aagcggtttg atcgattgat cctgccacat cagatcggat tgggcaggtc gaggaattca
     1741 tgttcgacct cgaagcgttg ggcgaccagg tcgccgaggg cggcaattcc gcagatctcg
     1801 gttgcgtagt ggccggccgc gatgaagtgg atcgcccctt cgcgggcgtc ggccatcaca
     1861 tgttccgagg gctcgccggt caggtaggcg tcgagcccga gcgcgatcgc ctcgtgaatg
     1921 tcggaagccc cggcaccggt gaccacgccg atggtcgaga ccatttccgg gccttcagcg
     1981 aacaccaggg gctcccggcc gagttcccgc ccgaccagct cgaaaagcgc agtcgccggg
     2041 atcggttccg cccgccggcc gatctgaccg atatggcggc ctttcgcttc gccgatctcg
     2101 ccgggaacgg gttcgaaacc gagctggcgg acgatcagcg cgttgttgcc gatttc
//
LOCUS       DAOQMF010000320         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_34051_length_2143_cov_3.628352, whole genome shotgun sequence.
ACCESSION   DAOQMF010000320 DAOQMF010000000
VERSION     DAOQMF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34051_length_2143_cov_3.628352"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLV77_06035"
     CDS             complement(<1..616)
                     /locus_tag="PLV77_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15440.1"
                     /translation="MNFPIRNLMTKSLAGALALAGLAAILLAPTASAAPIANAGEAPS
                     LLDFSGKAFKATPVAGATKAPQNPFMAKNQKSNIHNDTWMTGTYTWPGPLGRNLVTNS
                     GSAANGVCSTIAFDSHGDIVTVCVSTIAAPQARIVDPDTLEVIDTYDLPNADPVGNTP
                     AYQNFSAGGYFYLDNQDRLVIPTRNNHIYVLGQTPDGSQFQLVKD"
     gene            complement(613..1644)
                     /locus_tag="PLV77_06040"
     CDS             complement(613..1644)
                     /locus_tag="PLV77_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNC15441.1"
                     /translation="MPAGAPAAAKGSADRGASASVVGGSNASAASFPWQVLITANDEE
                     FCGGVLIHPKIVLTAAHCLVDDNWNYYEDLEGVTFRAFTGRTRLNSGGEELDWKVARA
                     DPDYDPDTNAYDWGFISLNSPASAPTLKLAGPNERSLWEPGRAATVSGFGDLSDGGQG
                     ATILQKAKIPLLADSGCSRYGASFHSSTQLCAGYLRGGRDACQGDSGGPLSVTADHGE
                     RRLVGLVSTGRGCAEAGFPGIYSKVAGAVNSDRIQQEVEFIETEDDFGAAYTGINVIG
                     SGARPLGCSAAVKSGKTAAQKLKSAQRRLKAARRSGRANRIEAARRGVERARHKLGRS
                     RVKSRKACT"
     gene            complement(1774..>2143)
                     /locus_tag="PLV77_06045"
     CDS             complement(1774..>2143)
                     /locus_tag="PLV77_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC15442.1"
                     /translation="VDASITYANERQAFGRNIGSFQAIRHKIAEMATTAAAAKTLTYD
                     TLRRFDEGEPVIREVSMTKLMTQRALCEVADEALQIHGGYGYMKEYGIERALRDARLG
                     PIGGGTDEIMKDIIGKTFGL"
BASE COUNT          325 a          666 c          739 g          413 t
ORIGIN      
        1 aatcctttac gagctggaac tggcttccgt cgggggtctg gccgagcacg tagatgtgat
       61 tgttgcgggt cggaatgacc agccggtcct ggttgtcgag atagaagtag ccgccggccg
      121 aaaagttctg gtacgccggg gtgtttccga ccgggtccgc attcggaagg tcgtaggtat
      181 cgatcacctc aagcgtgtcc gggtcgacga tccgggcctg tggtgcggca atcgtggaaa
      241 cgcagaccgt gacgatgtcg ccgtggctgt cgaaggcgat ggtcgagcag acgccgttcg
      301 cggcggaacc cgagttggtg accaggttcc ggccgagcgg gccgggccag gtgtaggtgc
      361 cggtcatcca ggtgtcgttg tggatgttgc tcttctggtt cttcgccatg aacgggttct
      421 gcggcgcctt cgtcgcgccg gcgaccgggg ttgccttgaa ggccttgccg gaaaagtcga
      481 gcagcgaggg ggcttcaccc gcgttggcga tcggcgcggc cgatgcggta ggggcaagga
      541 ggatcgcggc cagtccggcc agggccaggg cgccggccag cgacttcgtc atcagattgc
      601 ggatcgggaa gttcaagtgc atgcctttct cgatttcacc cggctcctgc cgagcttgtg
      661 cctggctctt tcaacacccc gccgcgcagc ttcgattcgg tttgccctcc ccgacctgcg
      721 agcggccttg agccttcgct gagctgactt tagcttctgc gcggcggttt ttcctgattt
      781 gaccgcggcc gaacacccga gcggcctggc accggagccg atcacgttga tcccggtgta
      841 ggcggcgccg aaatcatcct cggtttcgat gaactcgact tcctgctgga tgcggtcgct
      901 gttcacggcc ccggcgacct tcgagtagat gcccgggaag ccggcctcgg cacaaccccg
      961 tccggtgctg accaggccga ccagcctgcg ctcgccgtga tccgcggtga cgctcagcgg
     1021 ccctccgctg tcgccctggc aggcgtccct gccgcctcgc aggtagccgg cgcagagctg
     1081 cgtggaagag tggaaactcg ccccgtaccg ggaacatccg gagtcggcca gcagcgggat
     1141 cttcgccttc tggaggatcg tggcaccctg accgccgtcc gaaaggtcgc cgaagccgga
     1201 gaccgtcgcg gcccggccgg gctcccagag tgaacgctcg ttcggtccgg ccagcttcag
     1261 ggtcggggcg gaggcggggc tgttcagcga gatgaagccc cagtcgtagg cgttggtgtc
     1321 ggggtcgtaa tccgggtcgg cccgggcgac cttccagtcg agctcctcgc cgcccgagtt
     1381 cagacgggtg cgaccggtga aggcccggaa ggtgacccct tccaggtctt cgtagtagtt
     1441 ccagttgtca tcgaccagac agtgggcggc ggtgaggacg atcttcggat ggatcagcac
     1501 cccgccgcag aactcttcgt cgttggcggt gatcaggacc tgccagggga agctggccgc
     1561 gctggcgttg ctgccgccga cgacggacgc cgaggctccc cggtcagccg agcccttcgc
     1621 ggcggccggg gcacccgcgg gcagggtgag tcccgcaagc gtcagcgaaa gcgcaaggag
     1681 agcagccagt ttcgttccgg ttttcagggg ttctccgctt ggtcggtggt tcaggtgcga
     1741 ggtcccgctc ggggactaca gataacaacc cggctacagg ccaaaggtct tgccgatgat
     1801 gtccttcatg atttcgtcgg taccgccgcc gatcgggccg agccgggcat cccgcaaagc
     1861 cctttcgatc ccgtattcct tcatgtagcc gtagccaccg tgaatctgga gggcctcgtc
     1921 ggccacttcg cagagcgcgc gctgggtcat cagcttggtc atcgagactt cacggatcac
     1981 cggttcgcct tcgtcaaagc ggcgcagcgt gtcgtaggtg agagtctttg cggcggccgc
     2041 cgtggtcgcc atttcggcga tcttgtgccg gatcgcctgg aaggaaccga tgttccggcc
     2101 gaaggcctgg cgctcgttcg cataggtgat cgaagcgtcg acc
//
LOCUS       DAOQMF010000321         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_34078_length_2142_cov_3.263057, whole genome shotgun sequence.
ACCESSION   DAOQMF010000321 DAOQMF010000000
VERSION     DAOQMF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34078_length_2142_cov_3.263057"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /gene="pth"
                     /locus_tag="PLV77_06050"
     CDS             <1..391
                     /gene="pth"
                     /locus_tag="PLV77_06050"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNC15443.1"
                     /translation="LMTPLTYMNESGKSAGPARGSKKIPLDRVIAVYDEIDLPFGEIR
                     TRLGGGAAGHNGVKSLKQGFGSAGFWRVRIGVDRPDSTDPERVAAYVLGKFSESREQV
                     EELIDDAARETERLVERIAAGETVDGD"
     gene            413..1123
                     /locus_tag="PLV77_06055"
     CDS             413..1123
                     /locus_tag="PLV77_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC15444.1"
                     /translation="MSLTTYEVDDRGVALIQLDRPKAMNAINTEMLVELIEHVAVARS
                     DEGVKVLVFSSTDYMAFSAGADIKEDIDDEAKVNKMQLFADLYDAVVQFPKPTIAACH
                     GYVVGGGAEIAAACDIRIGGSNLQMRFPGAALGVPVGPARLVTLCGLSHAKYLLLSSN
                     TVKVDEALRMGLINQAAPGAATENTALELAGRIAAHDPAGVARLKRMLHEWDDIEGRS
                     EAEGRGQVEWQRSGPGLH"
     gene            complement(1265..>2142)
                     /locus_tag="PLV77_06060"
     CDS             complement(1265..>2142)
                     /locus_tag="PLV77_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15445.1"
                     /translation="EDLAEALSWRSVVQVCLGHRAEADADLARFDVLAKTLPQIRVNQ
                     AALHTARKVREGRWAEAEQLIAKTIAQEIPNTARIALTDPLLFMIRAEQNRLGEHFDQ
                     VKAMAAQSTSWEHWPTWRMGYYLAMTQCGRGDEVLERVDRFNQADVEAIETRNITFLS
                     FCALGSMVACEIEFRRGAETLVNLMEPYVGEWVITGPAGSALGPVEMHLGEICLLLGR
                     DREAATWLERSVDNCEGMGARPYLAHSKMHLALALRRLGNPEPERAEELMSSGQQIAE
                     ELDMQMLLNRINRWS"
BASE COUNT          372 a          684 c          712 g          374 t
ORIGIN      
        1 gctgatgacc ccgctcacct acatgaacga gtccgggaag tcggccggcc cggcccgcgg
       61 gtcaaagaag atcccgctcg accgggttat cgccgtctac gacgagatcg accttccctt
      121 cggtgagatc cgcacccgcc tcggcggcgg tgccgccggc cacaacgggg tcaagagcct
      181 gaaacagggt ttcggatcgg ccggtttctg gcgggtccgg atcggggtcg atcgacccga
      241 ttcgaccgat cccgaacgcg tcgcagccta cgtgctcggg aagttctccg agagccggga
      301 gcaggtcgag gaactgatcg atgatgcggc cagggaaacc gaaagacttg tcgagcgaat
      361 cgccgccggg gaaaccgttg acggcgactg aaccgaggaa agtgaagctg ccatgagcct
      421 gaccacctat gaagtagacg accgcggagt tgccctgatt cagctcgacc ggcccaaggc
      481 gatgaacgcg atcaacacgg aaatgctggt cgaactgatc gagcacgtcg cggtggcccg
      541 cagtgacgaa ggcgtaaagg tgctggtctt ctcctcgacc gattacatgg ccttctcggc
      601 cggcgccgac atcaaggaag acatcgatga cgaagccaag gtcaacaaga tgcagctttt
      661 cgcagacctc tacgacgcgg tcgtccagtt tcccaaaccg accatcgccg cctgccacgg
      721 ttatgtggtc gggggcggag cggagatcgc agccgcctgt gacatccgga tcggcggctc
      781 gaacctgcag atgcgcttcc ccggggcggc gctgggagtg ccggtcggcc cggcccggct
      841 ggtcaccctt tgcgggctca gccacgccaa gtacctgctg ctctcctcga acacggtcaa
      901 ggttgacgaa gccctgagga tgggcctgat caaccaggcc gcacccggcg ccgcaaccga
      961 gaacaccgcg ctcgagctgg ccggccggat cgccgcccat gaccccgcag gtgtcgcccg
     1021 attgaagcgg atgctccacg agtgggatga catcgagggg cggtcggaag ccgaaggccg
     1081 gggccaggtc gagtggcagc gttccggccc tggcttgcac tgagggcccg gtgtcggggg
     1141 tgcctgaaaa gtaacgagcg cttcgcgctc tgattaagga gtcactttta aggcaccccc
     1201 gacaccgggc cccgcattag ggagccggcg agcttcgctc cggctctgcc gcggttcggg
     1261 tctgtcagga ccagcggttt atgcggttca ggagcatttg catgtctagt tcttcggcta
     1321 tttgttggcc ggagctcatt agttcttcgg ctctttccgg ttcggggttg ccgaggcgcc
     1381 ggagggcaag agcgaggtgc atcttcgagt gggcgaggta gggccgggcg cccatccctt
     1441 cgcagttgtc gaccgagcgt tccagccagg tggcggcctc acggtcccgg cccagcagca
     1501 ggcagatctc gcccaggtgc atttccaccg ggccgagcgc cgaccctgcc gggccggtga
     1561 tcacccattc accgacgtaa ggctccatca gattgaccag ggtttccgcc cctcgcctga
     1621 actcgatctc acaggcgacc atcgagccga gcgcgcagaa tgagaggaag gtgatgttgc
     1681 gggtttcgat cgcttccacg tccgcctggt tgaaccggtc gaccctttcg aggacctcgt
     1741 ccccgcggcc gcactgggtc atcgccaggt agtagcccat ccgccaggtc ggccagtgct
     1801 cccagctcgt tgactgggca gccatcgcct tgacctggtc gaaatgctcg ccgagccggt
     1861 tctgctcggc ccggatcatg aagagcagcg ggtcggtcag ggcgatccgg gcggtgttcg
     1921 ggatctcctg cgcgatggtt ttcgcgatca gctgctcggc ctcggcccag cgtccttcgc
     1981 ggaccttccg ggcggtgtgg agggcggcct gattgacccg gatctggggc agggtcttgg
     2041 ccaggacatc gaagcgggcc aggtcggcat cggcctcggc ccggtgaccg agacagacct
     2101 gaacgaccga ccgccagctc agcgcttctg ccaggtcttc cg
//
LOCUS       DAOQMF010000322         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_34127_length_2141_cov_2.597315, whole genome shotgun sequence.
ACCESSION   DAOQMF010000322 DAOQMF010000000
VERSION     DAOQMF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34127_length_2141_cov_2.597315"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLV77_06065"
     CDS             complement(<1..297)
                     /locus_tag="PLV77_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNC15446.1"
                     /translation="MAEKFKAPRGTFDVLPEQAAQRERLLQAAREIFGLAGYRLIITP
                     AFEDTALFERGVGKSTDIVRKEMFTFEDGGGRSITLRPEGTAPVCRAYLEHGMQK"
     gene            complement(305..952)
                     /locus_tag="PLV77_06070"
     CDS             complement(305..952)
                     /locus_tag="PLV77_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC15447.1"
                     /translation="MAGLEVIGTTVGPVAENSYLVFKEETGKALLFDPGDEADRLMQP
                     LADRGLELEAILLTHCHFDHIGAVAPIARATGAPVYCPLIEVPILADVMAYVPWPGIG
                     PYESYDADHTVEGGEHLSLAGLEIDVLFTPGHSPGHVTYSIPEAELLLSGDVLFQGSV
                     GRVDLPGGDWDTLLESIRMLITTFPEQTRVLPGHMGVTSLGDELATNPFLTEIPR"
     gene            complement(962..1336)
                     /locus_tag="PLV77_06075"
     CDS             complement(962..1336)
                     /locus_tag="PLV77_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15448.1"
                     /translation="MAQTKKKRSRKKRGTQAGNLDTSRRSRPRSRAEARNQARSGGGK
                     GGRTMQKGDAPPTWRGATIRGLIAAGVFVLLLLLLFGRPLATSLIIGVVMLIFYIPAG
                     YYMDLYLWRRRERQRIRAKSKG"
     gene            complement(1360..1797)
                     /locus_tag="PLV77_06080"
     CDS             complement(1360..1797)
                     /locus_tag="PLV77_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15449.1"
                     /translation="MSRRAWQACRGRREPLVTARAPIAAFNLELATDDLAVAREIAAA
                     VREGPDDGIPGVRALGLLLSTGRAQVSTNVHDPIETPLAEIVDAVQEEATLRGTYVVE
                     AELIGLIPEAALEEYPEGVPIRDFDPAFHVIERRLKPRDQAGQ"
     gene            1883..>2141
                     /locus_tag="PLV77_06085"
     CDS             1883..>2141
                     /locus_tag="PLV77_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15450.1"
                     /translation="MPKKIVVLVNNPATHDTRVIKQASSLAAEGYEVVVIGRAAEGIP
                     DHETRDGVRYRRVPRVPFNYATCIIKRAADRTSAFRPVFLWA"
BASE COUNT          342 a          730 c          709 g          360 t
ORIGIN      
        1 cttctgcatg ccgtgctcga ggtaggcgcg gcagaccggc gcggtcccct ccggccgcag
       61 cgtgatcgag cggccgccgc cgtcctcgaa ggtgaacatc tccttgcgga cgatgtcggt
      121 cgacttgccg accccgcgct cgaacagggc cgtgtcctcg aaggccgggg tgatgatcag
      181 ccggtagccg gccagcccga agatctcgcg cgccgcctga agcagccgct cacgttgcgc
      241 ggcctgctcg ggcagcacat cgaaggtgcc gcgcggcgcc ttgaacttct cggccatcag
      301 ggtctcaccg ggggatctcg gtcaggaagg gattggtcgc cagctcgtcc ccgagactgg
      361 tcacacccat gtgcccgggc agcacccggg tctgttccgg gaaggtggtg atcagcatgc
      421 ggatcgattc gagcagcgtg tcccagtcac ccccgggcag gtcgacgcgg ccgaccgagc
      481 cctgaaagag cacgtcgccg gaaagcagca actccgcttc cgggatcgag taggtgacgt
      541 ggcccggact gtgaccgggt gtgaagagga cgtcgatttc aagtccggcc agcgagagat
      601 gctcgccgcc ttcgaccgtg tggtcggcgt cgtagctttc gtagggaccg attcccggcc
      661 acggcacgta ggccatcacg tcggccagga tcggcacctc gatcaacggg cagtagaccg
      721 gcgccccggt tgccctcgcg atcggggcca ccgccccgat gtgatcgaag tgacagtggg
      781 taagcaggat cgcttccagc tccagcccgc gatcggccag cggctgcatc agccggtcgg
      841 cctcgtcgcc ggggtcgaac aaaagcgcct tgccggtttc ctccttgaaa accaggtacg
      901 agttttccgc gaccggcccg accgtggttc cgatgacctc gagccccgcc atgccaggtg
      961 cctaaccctt gctcttggcg cggatccgct ggcgttcgcg ccggcgccag agatacaggt
     1021 ccatgtagta gccggccggg atgtagaaga tcagcatcac gacgccgatg atcagcgaag
     1081 tggcgagcgg ccggccgaaa agcagcagca ggagcaggac aaagacgccc gcagcgatca
     1141 gcccgcggat ggtcgctccc cgccaggtcg gcggagcgtc acccttctgc atggtccggc
     1201 cgcccttgcc gccgccggaa cgcgcctggt tcctggcttc ggctctggac cggggccgcg
     1261 aacggcgtga ggtgtcgagg ttgccggcct gggtgccgcg cttcttccgg ctgcgctttt
     1321 tcttggtctg tgccacgggc gaaacttacc ggagaacggc tactggccgg cttggtcgcg
     1381 aggcttcagg cgccgttcga tgacgtggaa ggccggatcg aaatcccgga tcggcacccc
     1441 ttccggatat tcctccagcg ccgcctcggg aatcaggccg atcaactccg cctcgaccac
     1501 gtaggttccc ctcagcgtcg cctcttcctg aaccgcatcg acgatctcgg ccaggggtgt
     1561 ttcgatcggg tcgtgaacgt tggtcgatac ctgggcccgc cccgtggaaa gcagcaggcc
     1621 gagggcgcgg acccccggga tgccgtcatc cggtccctcc ctgaccgcag ccgcgatttc
     1681 gcgtgcgacc gcgaggtcgt cggtggccag ttcgaggttg aaggcggcga tcggcgcccg
     1741 ggctgtgacc agcggttcgc ggcgaccacg gcacgcctgc caagctcgcc ggctcaattc
     1801 cctccgaccc cgattgtatc gccgaccgtc gcggtgatgc gcaggcatcg cccattcgaa
     1861 ccgggccgcc ccatacgatt ccatgcccaa gaagatcgtc gtactcgtca acaatcccgc
     1921 cacgcacgac acccgggtga tcaagcaggc gtcatcgctg gccgccgagg gttacgaggt
     1981 ggtggtcatc ggacgcgccg ccgagggcat tccggatcac gagacgcgcg atggcgtgcg
     2041 ttaccggcgc gtgccccgcg tgccgttcaa ctacgccacc tgcatcatca agcgggctgc
     2101 ggaccggact tccgcgttca ggccggtctt cctgtgggct t
//
LOCUS       DAOQMF010000323         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_34794_length_2117_cov_2.709020, whole genome shotgun sequence.
ACCESSION   DAOQMF010000323 DAOQMF010000000
VERSION     DAOQMF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34794_length_2117_cov_2.709020"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /gene="lspA"
                     /locus_tag="PLV77_06090"
     CDS             <1..492
                     /gene="lspA"
                     /locus_tag="PLV77_06090"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNC15451.1"
                     /translation="GYRHLIWAGLLALLVVILDQGAKALIVDNIDPGELVQVLGPLDL
                     THSHNDGVAFGLAGGGGILVILLAAVALVALGAFIASAPESNLTWISGGLILGGAAGN
                     LIDRVRIGHVTDFILLPSWPAFNLADCAITVGVVLLAVSLIWGEQEDRKEPGAAGPET
                     DGT"
     gene            482..1201
                     /locus_tag="PLV77_06095"
     CDS             482..1201
                     /locus_tag="PLV77_06095"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNC15452.1"
                     /translation="MAPEEIRSGEPGPQGFRIVFTDEFLAVIDKGWDLVVHPAPSHEG
                     VTLVELLGDELAGGDDPERPGIVHRLDRGTSGLMIVAREEQTHADLQKMIRDRQVSRI
                     YSALADGRFRSRTGTIDAPIGRASRKRHRMAVNGAASRDAVTHFEVVEALRRESLLEV
                     RLETGRTHQIRVHLEAIGHPVVGDATYGGPDRYGLSRQFLHSSRLEFTHPRTGAEMSF
                     TAPLPEDLATALEAARGADSL"
     gene            1334..>2117
                     /gene="rpsB"
                     /locus_tag="PLV77_06100"
     CDS             1334..>2117
                     /gene="rpsB"
                     /locus_tag="PLV77_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNC15453.1"
                     /translation="MSQVGIQELLEAGVHFGHQTSRWNPNMRRYIAGEIDGIHIIDLL
                     QTEEMLEEARRFVGELTSGGGKVLFVGTKKQASDVIEDWAGKSNMPFVNRRWLPGLLT
                     NFSVSSQRIKRMHEIRGLVESGQIELLPTKERMNMQAELAKLEFALGGVADMDRVPDA
                     VFITDLKSEEIALNEASRLRLPVIALVDSNCDPGHIDHVIPGNDDAIRSCELVISTIG
                     SAVEQGAGAWAVIEEKRRAEEQARREKEEAERKEEEAIIDLYK"
BASE COUNT          381 a          704 c          697 g          335 t
ORIGIN      
        1 ggttaccggc atctgatctg ggccggcctg ctggcgctgt tggtcgtcat ccttgaccag
       61 ggggccaagg ccctgatcgt cgacaacatc gacccgggag aactcgtcca ggtgctcggg
      121 ccgctggacc tgacccactc ccacaacgac ggggtcgcct tcggactggc cgggggaggc
      181 gggatcctgg tcatcctgct ggccgccgtg gccctggtcg cgctcggtgc cttcatcgcc
      241 tcggcgcccg aaagcaacct gacctggatc tccggggggc tgattctggg cggtgcggcc
      301 ggcaacctga tcgaccgcgt ccgaatcggc cacgtcaccg acttcatcct gctgccgagc
      361 tggcctgcct tcaacctggc cgattgcgca atcacggtcg gcgtggttct gctcgcggtc
      421 tcgctgatct ggggcgagca ggaagatcgc aaggaacccg gggcggccgg cccggaaacc
      481 gatggcacct gaggaaatca ggtccggtga gccgggccct cagggtttcc ggatcgtctt
      541 caccgatgaa ttcctggcgg tgatcgacaa gggctgggac ctggtcgtgc acccggcacc
      601 ctcgcacgaa ggagtgaccc tggtcgaact gctcggagat gagctggcgg gcggcgacga
      661 cccggagcgg cccgggatcg tccaccggct ggaccgcggc accagtggcc tgatgatcgt
      721 cgcccgcgag gaacagactc acgccgacct gcagaagatg atccgggacc gccaggtttc
      781 gcggatctat tcggcgctcg ccgacgggcg ttttcgatcc cgcaccggga ccattgacgc
      841 gccgatcggc cgcgcttcac gcaaacgcca ccgtatggct gtaaacggcg ccgcatcccg
      901 ggacgcggtc acccatttcg aggtggtcga ggcgctcagg cgcgaaagcc tgctggaagt
      961 gcgactggaa acggggcgga cccaccaaat ccgggttcac ctcgaggcga tcggccaccc
     1021 ggtggtcggg gacgcgacct acggcgggcc ggaccggtac gggctgagcc gccagttcct
     1081 gcattcgtca aggctcgaat tcacgcatcc ccggaccggc gcggaaatgt ccttcaccgc
     1141 accgttgccg gaggacctcg cgacggcgct cgaagccgca cgcggagcgg attccctcta
     1201 gactgggctg ctgtccgtat tccctaaccc ccgggatacg acggtggctc ctgccgcccg
     1261 gaacggttcc gaggcggatt ccaccgaaaa cggcccgggc ggatccgttt ttcaccccaa
     1321 ttcaaggaga agcatgtccc aggtaggaat ccaggaactc ctggaagccg gagtccactt
     1381 cggccaccag accagccgct ggaacccgaa tatgcgccgc tacatcgccg gcgagatcga
     1441 cgggatccac atcatcgacc tcttgcagac cgaggagatg ctcgaggaag cacgccgctt
     1501 cgtcggggaa ctcacctcgg gcggcggaaa ggtcctcttc gtcggcacca agaagcaggc
     1561 ctccgacgtg atcgaggact gggccgggaa gtcgaacatg ccgttcgtca accggcgctg
     1621 gctgccgggc cttctgacca acttcagcgt gtcctcgcag cggatcaagc gcatgcatga
     1681 gatccgcggc ctcgtcgagt ccggccagat cgaactgctg ccgaccaagg agcgcatgaa
     1741 catgcaggcc gagctggcca agctcgagtt cgccctcggc ggcgtcgcgg acatggaccg
     1801 ggttccggac gcggtcttca tcaccgacct caagtccgaa gagatcgccc tgaacgaggc
     1861 ttcgcgcctc cgcctgccgg tcatcgccct ggttgacagc aactgcgacc cgggtcacat
     1921 cgaccacgtg attcccggca acgacgacgc gatccgttcc tgtgaactgg tcatctcgac
     1981 catcggttcc gcggtcgagc agggtgccgg cgcctgggcc gtgatcgagg agaagcgccg
     2041 cgccgaagag caggcccgcc gggagaagga agaggccgag cggaaagagg aagaggcgat
     2101 catcgacctc tacaagg
//
LOCUS       DAOQMF010000324         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35021_length_2110_cov_3.157664, whole genome shotgun sequence.
ACCESSION   DAOQMF010000324 DAOQMF010000000
VERSION     DAOQMF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35021_length_2110_cov_3.157664"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLV77_06105"
     CDS             <1..346
                     /locus_tag="PLV77_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC15454.1"
                     /translation="EADLQPEMKPVRVTSQKVASQVADLMVAVVTSGTGIAAAIPEAQ
                     VAGKTGTAEVGPSGEVDANGDDILIEDAWFAGFAPSDKPQLAICVNVLDASGDGGTVA
                     APIAAAILSDGL"
     gene            complement(899..1252)
                     /locus_tag="PLV77_06110"
     CDS             complement(899..1252)
                     /locus_tag="PLV77_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15455.1"
                     /translation="MSDGPGHLRLADHGPALAGQIAAGLGAALALLGPFSDVIAGIGV
                     AILIIGVVLAAPAGRQPGPVMAEWWSVLALAALATLIGYGLAFWLPAPGGVLLTIGAV
                     TSLAAVFFGTPAQPE"
     gene            complement(1253..1810)
                     /locus_tag="PLV77_06115"
     CDS             complement(1253..1810)
                     /locus_tag="PLV77_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15456.1"
                     /translation="MERLSKSNLFRGLVLALVVAISGLALAACGASHEEKEGLAEGEI
                     VELGPLQYNVLFTRPLNRNDIEDSQYLVGKPAPGPDQMYIGVFVHVHNDSDDPQTIPD
                     SFQIETTAGRKYENIPSKSVYALQPGATVEPHDNIPQVDSTAQVGPIGASMLLFLIDR
                     ESAEQRPVELTIDGEDGPATFDLDL"
     gene            complement(1857..>2110)
                     /locus_tag="PLV77_06120"
     CDS             complement(1857..>2110)
                     /locus_tag="PLV77_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15457.1"
                     /translation="RNVTGTEWEMEEISTGVTRVRITFWIVPAGFSRVHNRLTGGAGW
                     HRRRLDKVGKRLREAIESARREKVEVPVAGGNRHATGVA"
BASE COUNT          335 a          692 c          731 g          352 t
ORIGIN      
        1 cgaagcggac ctgcagccgg aaatgaagcc ggtccgggtg accagccaga aggtcgcatc
       61 ccaggtcgcc gacctgatgg ttgccgtggt cacctcggga accggcatcg ccgccgccat
      121 tcccgaagcc caggtggccg gcaagaccgg caccgccgaa gtgggaccga gcggcgaggt
      181 ggacgcgaac ggtgatgaca tcctgatcga ggacgcctgg ttcgccggtt ttgccccctc
      241 cgacaagccg cagctcgcaa tctgcgtcaa cgtcctcgac gcctccggtg acggcggcac
      301 ggtcgccgcc ccgatcgccg ccgccatcct ctccgacggc ctttagtcct ctcgcgacaa
      361 gggaccccgc gagatcccac tttgtgccac ataccggcac aaaccgggca ctcgacggtt
      421 ccgccccaga ccggcccccg gtgggtcggt tttccctcac ctgccccgga aatccgaccc
      481 accacctggc gcgggaccgg tccgcaacgg ttcgtgggtc ggatttccgg tccagacgcc
      541 cgttatccga cccacctggg tggaaccggg caggcgacgg gggaacttca ggtgacccgg
      601 agttccgtgg tacctccaaa gccgggtcca ggcccaggtt tcgggaggtg ggtatggatt
      661 tcgtctatat aggcgaaatc catacccacc tcccggggtt cgggtgggga accggtcgag
      721 atcggagttg ggtgcgctat ccggcaagaa acgagatctc gaaggatgcc gggccctgcc
      781 gccgcgctgg gctcttggcg ctgcggggcc cttggcgctg cggggccccc ggcgctgcgg
      841 ggcccccggc gctgcggggt cttggcgttg cccgggggga tgggcgctgc ggggagtttt
      901 attcgggttg ggccggggtt ccgaagaaga ctgcggcgag ggaagtgacg gcgccgatcg
      961 tgaggagtac tcctccgggg gccggcagcc agaaggccag gccgtagccg atcagggtgg
     1021 ccagggcggc gagggcgagg accgaccacc actcggccat gaccgggccc ggctggcgac
     1081 cggccggggc ggcgaggacg actccgatga tcagaatcgc gacgccgatt ccggcgatca
     1141 cgtccgaaaa cggtcccagc agggccaggg ctgctccgag cccggccgca atctgaccgg
     1201 ccagcgccgg cccgtggtcc gccagcctga ggtggccggg accgtctgac acctagagat
     1261 cgaggtcgaa ggtggcgggg ccgtcttcgc cgtcgatcgt cagctcgacc ggccgctgtt
     1321 cggccgactc gcgatcaatc aggaagagca gcatcgaggc accgatcggt ccgacctggg
     1381 cggtcgaatc gacctgcggg atgttgtcgt ggggctcgac ggttgcgccg ggctgaaggg
     1441 cgtagacgct cttgctcggg atgttctcgt acttgcgacc ggcggtcgtc tcgatctgga
     1501 agctgtccgg aatcgtctgg ggatcgtcac tgtcgttgtg gacgtggacg aagaccccga
     1561 tgtacatctg gtcagggccc ggggccggct tgccgaccag gtactgcgaa tcctcgatgt
     1621 cgttcctgtt cagcggccgg gtgaagagca cgttgtactg cagcgggccg agctcgacga
     1681 tttcaccttc ggcgagaccc tccttctcct catggctggc accgcaagcg gcgagcgcga
     1741 gcccggagat agcgactacg agggccagga cgaggccacg aaacaggttg ctcttcgaaa
     1801 gtctttccat cggtgcggga gtttattcgg ccttatccgg acgtgctgag cggttcctag
     1861 gcgacaccgg tcgcgtgtcg gttgccgccg gcgaccggaa cctcgacctt ctccctgcga
     1921 gccgactcaa tcgcctcccg aagacgcttg ccgaccttgt ccagacggcg cctgtgccag
     1981 cccgcgccgc cggtcaggcg gttgtgaacc cgggagaatc cggccggcac gatccagaag
     2041 gtgatccgca cccgggttac cccggttgag atctcttcca tctcccactc ggtgccggtc
     2101 acgttgcggt
//
LOCUS       DAOQMF010000325         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35084_length_2108_cov_4.293717, whole genome shotgun sequence.
ACCESSION   DAOQMF010000325 DAOQMF010000000
VERSION     DAOQMF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35084_length_2108_cov_4.293717"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLV77_06125"
     CDS             complement(<1..333)
                     /locus_tag="PLV77_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNC15458.1"
                     /translation="MAEALIIDIDGTLVDSNYQHVIAWQEALREHGLDAEAWKIHRYV
                     GKGGDKMIESVAGEEAERKAGDAVRESEGEIFQRLIDQVRPLEGAGEFVRKVKDLGFI
                     VVLSSSAKD"
     gene            complement(338..817)
                     /locus_tag="PLV77_06130"
     CDS             complement(338..817)
                     /locus_tag="PLV77_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15459.1"
                     /translation="MAEISTVEEKLAEVTGLAQAAQGAIEKVAPMIDDESVSESLEKM
                     KQEAVETEERCTSAIEKRDGRKTAILDKARETKQEATEMMSTYLGDDSDGLDGLEFMT
                     MAEAGEVGHWEILGELSQRAGESELEELTGWALPIQERHLAEVRKGSLTLASQEDID"
     gene            complement(823..1266)
                     /locus_tag="PLV77_06135"
     CDS             complement(823..1266)
                     /locus_tag="PLV77_06135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNC15460.1"
                     /translation="MIVFLVVDMISRWDFDGADRAADRAGDAVEAINRLRRRAGENLK
                     VVYANDMDGGFHGSRETAFALAMSGRHPELVSPLEPRPDEDFIHKGQHSAFYGTPLAH
                     LLDVCDVETIWLAGQATEQCILYTALDAHVRGSGYYRSQSTPYWR"
     gene            complement(1293..1559)
                     /locus_tag="PLV77_06140"
     CDS             complement(1293..1559)
                     /locus_tag="PLV77_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNC15461.1"
                     /translation="MPKGSDHGPSVKDDKLYEELREDGASKEKAARIANAKAGGENVS
                     KKGGESEDYEDRTVEELQDRAAEIGIEGRSDMNKKELISALRNH"
     gene            1679..>2108
                     /locus_tag="PLV77_06145"
     CDS             1679..>2108
                     /locus_tag="PLV77_06145"
                     /inference="COORDINATES: protein motif:HMM:NF039409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HNC15462.1"
                     /translation="MGSSPRDEENWQRLDRNLDELLQELRIALPGVQVLFAFLLAVPF
                     QQRFGDVTDFQQSVYFATLMLTGASTFLLIAPTAFHRVTFRQQQKERLVRLANRLAIA
                     GLGCLGLAVIGAVTLVTDFLYGAAPTALAAGAFLVIATVLW"
BASE COUNT          293 a          698 c          623 g          494 t
ORIGIN      
        1 gtccttggcc gaactcgaaa gcacgacgat gaagccgagg tccttcacct ttcgcacgaa
       61 ttcgccggcg ccttccagcg gcctgacctg atcgatcagc cgctggaaga tctctccttc
      121 actttcgcgg accgcgtcgc cggctttccg ctcggcttcc tcgccggcaa cggattcgat
      181 catcttgtcg ccacccttgc ccacgtagcg atggatcttc cacgcttcgg cgtcgagccc
      241 gtgctctctc agggcttcct gccaggcgat cacgtgctgg tagttggagt cgaccagggt
      301 gccgtcgatg tcgatgatca gagcttctgc catgaagtca gtcgatgtct tcctggctgg
      361 caagggtcag cgagcccttc cttacttcgg ccaggtgacg ctcctggatc ggcagcgccc
      421 atcccgtgag ttcctcaagt tcggattctc cggcccgctg actgagttca cccaggatct
      481 cccagtggcc cacctcgccg gcctcggcca tggtcatgaa ctcgagtccg tcgagcccgt
      541 ccgagtcgtc accgaggtag gtcgacatca tctcggtcgc ttcctgcttg gtttcgcggg
      601 ccttgtcgag aatcgcggtc ttgcgaccgt cccgcttctc gatcgcgctg gtgcaccgct
      661 cctcggtctc gaccgcttcc tgcttcatct tctccaggct ctccgacacc gattcgtcgt
      721 cgatcatcgg tgcgaccttc tcgatcgctc cctgggctgc ctgggccagg ccagtgacct
      781 cggcgagctt ctcctcgacc gtcgatattt ctgccatttc gattacctcc agtaaggggt
      841 tgactggctg cggtagtacc cgctgcccct gacgtgggcg tccagtgccg tgtagaggat
      901 gcactgctcg gtcgcctggc cggccagcca gatcgtttcc acgtcgcaga cgtcgagcag
      961 gtgtgccagc ggcgtgccgt agaaggccga gtgctgaccc ttgtggatga agtcctcatc
     1021 gggacggggt tcgaggggtg aaaccagttc cgggtgccgg cccgacatcg ccagggcaaa
     1081 agctgtttcc cgggatccgt ggaagccccc gtccatgtcg tttgcgtaaa cgaccttcag
     1141 gttctccccg gcacggcggc gcagccggtt gatcgcttcg actgcgtcac cggcccgatc
     1201 cgccgcccgg tccgcaccgt cgaaatccca gcgagagatc atgtcgacga cgaggaatac
     1261 gatcatcgcg ctggccgtcc ggccgtcagg ggtcagtgat tgcgcagggc gctgatcagt
     1321 tccttcttgt tcatgtcgga gcggccttct atgccgatct cggcggcccg gtcctgcagt
     1381 tcctcgacgg tgcgatcctc gtaatcctcg gattcgccgc ctttcttgct tacgttctct
     1441 ccgcccgcct tcgcgttggc gatccgggcc gctttttcct tgctggcgcc gtcttcgcgg
     1501 agttcttcgt agagcttgtc gtccttgacg ctcggtccgt ggtctgatcc tttcggcatt
     1561 tcgagtctcc tttgcgttcg aatttgccag tcaaccttgg ctaccgtttc tggtacccga
     1621 cggttgcgcg tcaaaaccag tccggttcga gtcgaggccg tccgggtacc tcgggggtat
     1681 gggatcgagc ccgcgcgatg aggagaattg gcaacgcctt gaccggaacc tggacgagtt
     1741 gctccaggaa ctcaggatcg ccctgcccgg agtccaggtc ctcttcgcgt ttctgctggc
     1801 ggttccgttc cagcagcgtt tcggcgatgt gaccgatttt cagcagtcgg tctatttcgc
     1861 gaccctgatg ctgacggggg cctcgacctt tctgttgatc gccccgaccg cgtttcaccg
     1921 cgtgacattt cgccagcagc agaaggaacg cctcgtcaga cttgccaatc gcctggccat
     1981 tgcagggctc ggctgcctcg gtctggccgt gatcggcgcg gtgacgctgg ttaccgactt
     2041 tctctacggc gcggccccca cggccctcgc ggcaggagcc ttcctggtga tcgcgaccgt
     2101 gttgtggt
//
LOCUS       DAOQMF010000326         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35210_length_2104_cov_3.096144, whole genome shotgun sequence.
ACCESSION   DAOQMF010000326 DAOQMF010000000
VERSION     DAOQMF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35210_length_2104_cov_3.096144"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PLV77_06150"
     CDS             <1..941
                     /locus_tag="PLV77_06150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC15463.1"
                     /translation="MRRLRRRLENAPDPMVTHYQWLTMPRFDHRLISKRRPRLMTAHY
                     ILPPGPNGKQAAAAKRVFGAMDAVIAHSKGGASRLVEEVGLPEERVRVIPHGAFDYLT
                     RLPEEAPLPPELADSGAEAPVILFFGLLRPYKGIDVLIRAAGQLAADADRDGPAPELW
                     IVGNPRMDLTELVERSRKLKVKVRWLPRFIEDQEIPAIMRRADVVVLPYLDGEQSGVL
                     YTGIAFGKAMVVSDVGGIGEVAREHGLARTVEPGDVDQLAAELSALVLGDRAAAAREE
                     LETRCRVAAEGPFAWAAVARQTIALYEDLLGRGSGS"
     gene            938..>2104
                     /locus_tag="PLV77_06155"
     CDS             938..>2104
                     /locus_tag="PLV77_06155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC15464.1"
                     /translation="MIVVYILFWLSAGLVFFTHIGYPITLWLLDFIGLGGRAGEVRGR
                     KLSEIPPAIDPDTEEEILPSVSLIIAAYNEETVIEAKVKNALELEYPRELLQVIVASD
                     GSSDRTVEIAREAGADLVLDLPRGGKIAAQNAAAEAATGNLLAFSDANSTWEPGALAE
                     LIDPFRDERVGYVCGQVRFIGPDGGNLEGAYWRYEMRVREMESELAGVTAGNGAIYAV
                     RASAYIPLAPSGSHDLSFPFALAKRRLASLYRPRARATEKLVPSLDGELARKRRMMVG
                     LWDIVVGEGMLDPRGYSPLYLFELFSHRLLRYLSPALHLIAFLTNLALLGHGWFYGLT
                     MLLQLLLVAGAILGRWIPILPFRISRYYLEVTLSIALGLWDRWRQGPPAAWEKAE"
BASE COUNT          316 a          723 c          705 g          360 t
ORIGIN      
        1 agatgcgccg cctccggcgc aggctggaga acgccccgga cccgatggtc acccattacc
       61 agtggctgac catgccgcgg ttcgatcatc gactgatctc gaagcgccgt ccccgtttga
      121 tgaccgccca ttacatcctg ccgcccggcc cgaacgggaa gcaggccgcg gccgcgaaga
      181 gggttttcgg cgcaatggac gcggtgatcg cccattcgaa gggcggggcg agccgactgg
      241 tcgaagaggt cggcctgccg gaggagcggg tgcgggtgat ccctcacggc gccttcgact
      301 acctgacccg gctgccggag gaagccccgt tgcccccgga actggccgac tccggtgccg
      361 aggctccggt catcctcttc ttcggcctgc ttcgccccta caagggaatc gacgtgctga
      421 tccgcgcggc cggacagctg gccgcggatg ccgaccgaga cgggcccgct cctgagcttt
      481 ggattgtcgg caatccgcgc atggacctga ccgaactggt cgaacgctcg aggaagctga
      541 aggtcaaggt ccgctggctg ccccgcttca tcgaggatca ggagatcccg gcgatcatgc
      601 gacgggccga tgtcgtcgtc ctgccatacc tcgatggcga gcagtcgggt gtgttgtaca
      661 cggggatcgc cttcggcaag gcgatggtcg tctccgacgt gggcggtatc ggcgaggtcg
      721 cccgggaaca cgggcttgcc cggaccgtcg agccgggtga tgtcgaccag ctggccgccg
      781 agctttcggc gttggtcctc ggggaccggg ccgctgcggc cagggaagaa ctcgagacga
      841 ggtgcagggt cgcggcggag ggacccttcg cctgggccgc cgttgcccga cagacgatcg
      901 cgctctacga ggacctgctc ggccggggga gtggatcgtg atcgtcgtct acatcctctt
      961 ctggctcagc gcgggcctgg tcttcttcac ccacatcggc tacccgatca cgctctggct
     1021 gctcgacttc atcggcctgg gcggccgcgc cggggaggtg cggggccgca agctcagcga
     1081 gattccgccg gcaatcgatc ccgacaccga ggaggagatt ctgccttcgg tctcgctgat
     1141 catcgccgcc tacaacgagg agacggtgat cgaggcgaag gtgaagaacg cgctggagct
     1201 tgagtatccg cgcgagctcc ttcaggtcat cgtcgcctcg gacggctcat cggatcgcac
     1261 cgtggagatc gcccgggagg ccggcgccga ccttgtcctg gatcttcccc ggggcggcaa
     1321 gatcgccgcg cagaatgccg cggccgaagc ggcgaccggg aacctcctgg ccttctcgga
     1381 tgcgaactcg acctgggaac cgggcgcgct cgccgaactg atcgacccct tccgcgacga
     1441 gcgagtcgga tatgtctgtg gtcaggtccg cttcatcgga cccgacggcg gcaacctcga
     1501 aggcgcctac tggcgctacg agatgcgggt ccgcgagatg gagtcggagc tggccggggt
     1561 caccgccgga aacggcgcga tctacgcggt ccgggccagt gcctacatcc ccctcgcccc
     1621 ctcgggcagc cacgaccttt cgttcccctt cgccctggcc aagcgaaggc tcgcttcgct
     1681 ttaccggccc cgggcccggg caaccgagaa gctggtcccc agcctcgacg gcgaactggc
     1741 ccgcaagcgg cggatgatgg tcggcctctg ggacatcgtg gtcggggagg gaatgctcga
     1801 cccgcggggc tactcgccgc tctacctctt cgaactcttc tcgcaccgac tgctccgcta
     1861 cctcagcccg gcccttcacc tgatcgcctt cctgaccaac ctcgcgctgc tcggccacgg
     1921 ctggttttac gggctgacca tgctgcttca gctgctgctc gtggccgggg cgattctcgg
     1981 tcgctggatc ccgatcctgc ctttccgcat cagtcgctac tacctcgagg tgaccctctc
     2041 gatcgcgctc ggcctctggg accgctggcg ccagggcccg ccggccgcct gggagaaagc
     2101 cgag
//
LOCUS       DAOQMF010000327         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35214_length_2104_cov_3.004880, whole genome shotgun sequence.
ACCESSION   DAOQMF010000327 DAOQMF010000000
VERSION     DAOQMF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35214_length_2104_cov_3.004880"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLV77_06160"
     CDS             complement(<1..306)
                     /locus_tag="PLV77_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNC15465.1"
                     /translation="MPELPEVETIRRQLEPDLNGRVIETVEVLDPYFTKPDLPGNFER
                     AVNGREIVDVGRRGKYLLLELDDGLTVVIHLRMTGSLVLLAPDGSENPGDARIYEADR
                     "
     gene            complement(468..1433)
                     /gene="xerD"
                     /locus_tag="PLV77_06165"
     CDS             complement(468..1433)
                     /gene="xerD"
                     /locus_tag="PLV77_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNC15466.1"
                     /translation="MPPATVTSVPAPEVLEGARFEGLLLEFLAYLELERGLSRNTLNA
                     YRTDLLQYGDYLRAKGIDPLEIDAAGMSEYVADLIEGKGSGHRLAASTVHRKTAALRS
                     FYKHLRLEDLVGDDPTAGLSTPRRSKKLPNVLNQAEVTRLLAVPAGDSPQAYRDRAIL
                     EVMYACGLRASELIDLEMTDVDTYEGMLRARGKGSKERIVPLGRQAIYALEGYLLSGR
                     PDLVKGRSEKALFLNFRGRRLTRQGLYKIVQGHAKSAGLEDRMSPHTLRHSFATHLLA
                     GGCDLRAVQEMLGHADVATTQMYTHLTGQQLRDVYYAAHPRAQAN"
     gene            complement(1523..>2104)
                     /locus_tag="PLV77_06170"
     CDS             complement(1523..>2104)
                     /locus_tag="PLV77_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HNC15467.1"
                     /translation="GGALPGSLSRDVSELLDRGLLAGHVTAGPAYGGGGEAISLVGAI
                     DAASSLGWGGVIAGPGPGILGSGTAFGHGGMAALDVIHASLALGAPVLLSPRLSAGDP
                     RPRHRGLSHHSRSVLDLALGPVEFPVPAGFLDLIPEGTPGAKSVEVNLEGYGESGLPQ
                     KTMGRDLEEDRPFFAGPLAAGTRLGQLIAEPAT"
BASE COUNT          369 a          692 c          674 g          369 t
ORIGIN      
        1 ccggtcggcc tcgtagatcc tggcgtcacc cgggttctcg ctgccgtccg gtgccagcaa
       61 gaccagggag ccggtcattc gcagatggat gaccacggtc aagccgtcgt cgagttcgag
      121 cagcaggtat ttgccgcgcc gccccacatc cacgatctcc cgtccgttca cggcccgctc
      181 gaagttcccg ggcagatcgg gcttggtgaa gtaggggtcg agcacctcaa ccgtttcgat
      241 cacccgcccg ttcagatcag gctcaagctg gcgacgaatg gtctcgactt caggaagctc
      301 gggcacccgc cggttgtatc agagccctcc cctcggcagt gggtcggatt tccggcacag
      361 gcaccggttt tccgacccac taccgcgccg gtgagtggtt cgtaaaccag gtgggtcgga
      421 ttctcggcgt gtggaccgga aatccgaccc accggggatt tggcttctca gttagcctgg
      481 gctctggggt gggcggcgta atagacgtcg cggagctgct ggccggtcaa gtgggtgtac
      541 atctgggtcg tggcaacatc ggcgtggccg agcatctcct gtaccgccct caggtcacag
      601 ccaccggcga gcaggtgggt ggcgaaggag tggcgcaggg tgtgagggct cattcggtct
      661 tcgaggccag ccgatttcgc atgcccctgc acgatcttgt agagaccctg cctggtcagg
      721 cggcggcccc gaaagttgag aaaaagcgcc ttttcggatc gccccttgac cagatccggc
      781 cgacccgaaa ggaggtaccc ctccagagcg tagatcgcct gtcgaccaag cggcacaatt
      841 ctttccttgc tgcccttgcc cctcgcccgc agcatcccct cgtaggtgtc gacgtcggtc
      901 atctcgaggt cgatcaactc ggaggcccgc agtccgcagg cgtacataac ttcgaggatc
      961 gcccgatccc ggtaagcctg cggagaatct cctgccggaa ccgcgagaag tcgtgtcacc
     1021 tcggcctggt tgaggacatt gggcagcttc ttcgagcgac gaggtgtgct cagcccggcg
     1081 gtcgggtcgt ccccgaccag atcctcgagt cgcagatgct tgtaaaacga acgcagggcg
     1141 gcggtcttgc gatgaacggt cgaagcagcg agtcggtggc cggaaccttt gccctcgatc
     1201 aggtcggcca cgtattcgga cattccggct gcgtcgatct caagcggatc gatccctttt
     1261 gcccgcagat agtcaccgta ctgcagcagg tcggtgcggt aggcattcag ggtgttgcgc
     1321 gaaagacccc gctccagttc aagataggcg aggaactcga gcagcaggcc ttcgaaacgg
     1381 gcgccttcga gtacttccgg agcagggacg gacgtgacgg ttgccggggg catgaaagcg
     1441 ctttctcccc cacccgcttc atcccttgcc ccgcaacgaa acctccgacc ctgtaaccag
     1501 gcgaatcgcg ggcaggcggc gatcaggttg ccggttcggc gatcagctgg ccgagtcggg
     1561 tgccagcggc gagcggaccg gcgaagaagg gcctgtcttc ttccagatcg cggcccatcg
     1621 ttttttgggg cagtcccgac tcgccatacc cctcaagatt gacttcgacc gacttcgctc
     1681 cgggagtccc ctcgggaatc aggtcgagga acccggccgg gaccggaaac tccaccggac
     1741 cgagagcgag atccagaacg gaccggctgt ggtggcttag cccccgatgt cgcggccgcg
     1801 ggtcacccgc cgagagtctc ggtgaaagca gcactggagc tcccagtgcc agactggcgt
     1861 gaatcacgtc aagcgccgcc attccgccat ggccgaaagc ggtaccggag ccgaggatcc
     1921 cgggccccgg cccggctatc accccgcccc atccgagcga ggaggcggcg tcgatcgccc
     1981 cgaccaggga gattgcctct cctcccccgc cgtaggccgg tccggcggtc acatgcccgg
     2041 ccagaaggcc ccggtcgagc agttccgaca cgtcccgcga aagggacccg ggcagcgccc
     2101 cgcc
//
LOCUS       DAOQMF010000328         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35309_length_2101_cov_2.993157, whole genome shotgun sequence.
ACCESSION   DAOQMF010000328 DAOQMF010000000
VERSION     DAOQMF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35309_length_2101_cov_2.993157"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLV77_06175"
     CDS             complement(<1..218)
                     /locus_tag="PLV77_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15468.1"
                     /translation="MASASATAAARPHVVTVRAPQKMAQGSAYVVATVIRNPAAKIAR
                     GSIRITLSTNRGSGPWLLGRREVAGVAP"
     gene            complement(379..1227)
                     /locus_tag="PLV77_06180"
     CDS             complement(379..1227)
                     /locus_tag="PLV77_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC15469.1"
                     /translation="MGENVRSEIFREDLLEGKVVVLSGAGTGLGREATLEMIRLGATV
                     VGCGRRAEPLEETAKLAGNLPGAFEHDTLDIRDEEGVDSFFDGVMERHGRIDTLVNNA
                     GGQFLTPAEAITPKGFRTVIELNVTGTWLMTHAAATKAFIPQGEGAVISVTLSPHNGM
                     PGMVHSGAARAAVENMMKTLSIEWARFGIRTCAIAAGQFATDTFVNKYPKEIVDVVHR
                     TIPMGRVGESEEMGWLIAFLASGAGAFHSGSVITLDGARDNWFGPWPPPSITGESGTP
                     PAEERR"
     gene            1278..>2101
                     /locus_tag="PLV77_06185"
     CDS             1278..>2101
                     /locus_tag="PLV77_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HNC15470.1"
                     /translation="MSIHAESRPLETPLPGGKEGATVTVEPMVTGTFSSPPILHDSPK
                     GKAGTLAILRSTRKEREPTPIPCFLIRHPSAGLILVDTGLHPSIASDPASNLGKFTTW
                     LMRPHQEPGQDVLSQLREKGINPADIRMVIFTHLHYDHASAISEFPEATLIVNDVEWK
                     YAATVRSPDFKGYRRQQFDYAFDFRTISFDSDKVSSYASFGRTLDLFGDGSIRLASTP
                     GHSPGHQSLICRLGDRDLVISGDAIYEESKLEAGANLPGTMFDEHNYKRSLSELKR"
BASE COUNT          368 a          723 c          640 g          370 t
ORIGIN      
        1 ctcggcgcga ccccggccac ctcgcggcgg ccaagcagcc aagggcccga accgcgattc
       61 gtcgagagcg tgatcctgat cgaaccccgg gcgatcttcg cggccgggtt gcgaatcacc
      121 gtggccacga cataggcact tccctgggcc atcttctgtg gtgcccggac cgtgaccacg
      181 tgtggtctgg ccgcagccgt cgcagaggcg gaggccatcg ctccgaacag caacaggagc
      241 gtcagcacaa cagaccaccc gatcggcctc ctggtccgtg cttcgttgaa gtccatcgtc
      301 cagctccttc ccgcgcctct gccagccttc gtgatcagca taacgccggc cacatcggtt
      361 ccgggcgatt cgggagcgtt agcggcgttc ttcggccggc ggggtgccgc tctcgccggt
      421 tatcgagggc ggcggccagg gcccgaacca gttgtcgcgg gcgccgtcca gggtgatcac
      481 cgacccggag tggaaggcac cggcaccgga ggcgaggaag gcgatcagcc agcccatctc
      541 ctcggattcc ccaacccgtc ccatcgggat ggtccggtga accacatcga cgatctcctt
      601 cgggtacttg ttcacgaagg tgtcggtggc gaactgaccg gcagcgatcg cgcaggtcct
      661 gatgccgaat ctcgcccatt cgatcgaaag cgtcttcatc atgttctcca ccgcggcccg
      721 ggcggccccc gagtgaacca tgccgggcat gccattgtgg ggagacaggg tgacgctgat
      781 caccgcacct tccccctggg gaatgaaggc cttggtcgcg gcggcgtggg tcatcagcca
      841 ggtgccggtc acgttcagct cgatcaccgt gcgaaagccc ttgggggtga tcgcctcggc
      901 cggggtcagg aactggccgc cggcgttgtt gaccagggtg tcgatccggc cgtgacgctc
      961 catcacaccg tcaaagaagg aatcgactcc ttcctcgtcg cggatgtcaa gggtgtcgtg
     1021 ttcgaaggcg cccggcagat tcccggccag cttcgcggtc tcttcaagcg gctccgctcg
     1081 tcggccgcaa ccgaccacgg tcgcccccag ccggatcatt tccagggtgg cttcacggcc
     1141 gaggccggtt ccggctccgg agagcacgac caccttgccc tcgagcaggt cttcacggaa
     1201 aatctctgag cgtacgtttt ctcccattgg ctgccgcgaa tcctatccct gagccgtccg
     1261 gcggtaaggt ccggcaggtg agcatccacg ctgagtcgag accgctcgaa acgccgcttc
     1321 ccggcgggaa ggagggcgcg accgtaacgg tcgagccgat ggtgacgggc accttcagca
     1381 gcccgccgat ccttcacgac agcccgaaag gcaaggcggg gactctggcc atcctccgat
     1441 cgacccgcaa ggaacgggaa ccgaccccga ttccctgttt cctgattcgc cacccttccg
     1501 ccggactgat cctggtcgac acgggacttc acccgtcgat cgcttccgac ccggcttcca
     1561 acctcggcaa gttcacgacc tggttgatgc gccctcatca ggagcctggt caggacgttc
     1621 tctcccagct gcgagagaag ggcatcaacc ccgccgacat ccggatggtg atcttcactc
     1681 accttcacta tgaccacgcc tcggcgatct ccgaattccc ggaagcgacc ctgatcgtca
     1741 atgacgtcga gtggaagtat gcggcgaccg tccgcagccc ggacttcaag ggttaccgcc
     1801 gccagcagtt cgattacgcc ttcgacttcc ggacgatctc cttcgattcc gacaaggtca
     1861 gctcgtacgc aagcttcggc cgcaccctcg accttttcgg ggacgggtcg atccgcctgg
     1921 cctcgactcc gggtcactca cccggccacc agtcgctgat ctgccgcctg ggggaccggg
     1981 atctggtgat cagcggcgac gcgatctatg aagaaagcaa gctcgaagcc ggagcgaacc
     2041 tgccgggcac gatgttcgac gagcacaact acaagcgcag cctcagcgag ctgaagcgct
     2101 t
//
LOCUS       DAOQMF010000329         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35356_length_2099_cov_3.911937, whole genome shotgun sequence.
ACCESSION   DAOQMF010000329 DAOQMF010000000
VERSION     DAOQMF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35356_length_2099_cov_3.911937"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1059
                     /gene="nadC"
                     /locus_tag="PLV77_06190"
     CDS             199..1059
                     /gene="nadC"
                     /locus_tag="PLV77_06190"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNC15471.1"
                     /translation="MNHLPEGTAELIARALAEDIGPGDVTANATVPADLAAQAHIVLK
                     QPGVIYGLDVVEEVFRQVGVTDIDRTVVEGHWHDSVPRDILLINGQARAILAGERVAL
                     NFLGHLSGIATLTARYVDAVGGTGTRILDTRKTLPGLRALEKQAVSWGGGINHRFGLF
                     DAVLIKENHVALAGGVREAIESCRRESPDLLIEVEAEDLDQVAEAIEAGADRILLDNM
                     DIESLKRAVDLRESGGPGTELEASGGMNLETVRAVAETGVDFISIGALTHSAPQLDVS
                     LLLDPVNRLP"
     gene            1070..>2099
                     /gene="nadA"
                     /locus_tag="PLV77_06195"
     CDS             1070..>2099
                     /gene="nadA"
                     /locus_tag="PLV77_06195"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNC15472.1"
                     /translation="MPTELPVLQASDVPPPLDPAEIKELSAEIRELAAERNAIILAHN
                     YELPEVQDVADYIGDSLGLSREAEKADAEVVEFCGVHFMAETASILSPEKKVLIPDLE
                     AGCSLSDSINADQLREWKAKFPGAMVVMYVNTSAEVKAETDYCCTSSNAVKVVEHIWA
                     EHGEDTEILFGPDMWLGAFVERETGLIEDPERRSRFHVWDGECHVHAGIRPDDIARTR
                     AEHPGAEFLIHPECGCSTQAMEYVATGDIDPEGVHMLSTSGMIEHVRENPEGEYIVAT
                     ENGMLYPMKQAVPGARLIEANRMAYCKYMKMITLPKLRDSLRDMKFEVKVDEALAEKA
                     RLPIERMVS"
BASE COUNT          406 a          668 c          687 g          338 t
ORIGIN      
        1 gatcgaccgt cctgccgccc tcgaccgggg tgagcccggt tttcgtggcc acttcctcga
       61 ccagggcatc agggtccgag cccggcaggt tgcagcgaac cacgcctgtt tccgtggcgg
      121 ccagggccac cgggcccagc ggcgtgtcaa aagcggtgtg agtgaacgaa tccaccatgg
      181 agagcatagg ctccctgcat gaatcacctg cccgaaggaa ccgcggagct gatcgcccgg
      241 gccctggccg aagacatcgg ccccggtgac gtgaccgcga acgcgacggt tccggccgac
      301 ctggcggcgc aggcccacat cgtcctcaag cagccagggg tcatctacgg actggacgtg
      361 gtcgaggaag tcttccggca ggtcggggtg accgacatcg atcgcaccgt ggtcgaaggt
      421 cactggcacg attcggtccc gcgcgacatc ctgttgatca acggccaggc cagggcgatc
      481 ctcgccgggg aaagggttgc gctgaacttt ctcggtcacc tctccggcat tgcgaccctg
      541 accgcgaggt acgtcgacgc agtgggcgga accggcaccc ggatcctcga cacgcgcaag
      601 acactgccgg gcctgcgggc gctggagaaa caggcggtct cctggggcgg tggcatcaat
      661 caccgcttcg gactcttcga cgcggtgctg atcaaggaga accacgtcgc cctggcgggc
      721 ggggtccggg aagcgatcga gtcctgccgc cgcgaatccc cggacctgct catcgaagtc
      781 gaagccgagg atctcgatca ggttgccgag gcgatcgaag cgggcgccga ccgcatcctg
      841 ctggacaaca tggacatcga atctctcaag cgggccgtgg acctgcgtga atcgggcggt
      901 ccgggaaccg aactcgaagc ctcgggcggg atgaacctgg aaacggtccg ggccgtagcc
      961 gagaccgggg tggacttcat ttcgataggt gccctgaccc actcagctcc gcaactggat
     1021 gtttcgctgc tgctcgatcc cgtgaaccgg cttccgtaga attgttgcca tgcccaccga
     1081 acttccagtc ctccaggcat cggatgtgcc gccgccgctc gatccggccg aaatcaagga
     1141 actgagcgcg gagatccgcg aactcgcggc cgaacggaac gcgatcatcc tcgcccacaa
     1201 ctacgagctt cccgaagtcc aggacgtggc ggactacatc ggtgactcgc tgggcctttc
     1261 ccgcgaggcg gagaaggcag atgccgaggt ggtcgagttc tgcggagtcc acttcatggc
     1321 cgagacggcg tcgatccttt cgccggaaaa gaaggtgctg atccccgatc tggaggcagg
     1381 ctgctcgctt tcggattcga tcaacgccga tcagttgcgg gaatggaagg cgaaattccc
     1441 cggggcgatg gtcgtcatgt acgtcaacac ctcggctgaa gtcaaggcgg aaaccgatta
     1501 ctgctgcacc tcctcgaatg cggtcaaggt ggtcgagcac atctgggccg agcacggcga
     1561 ggacaccgag atcctcttcg gaccggacat gtggctcggt gccttcgttg aacgcgagac
     1621 cgggctgatc gaggaccccg agaggcgttc acgcttccat gtctgggacg gcgaatgcca
     1681 cgtccacgcc ggcatccggc cggatgacat cgcccgcacc cgggccgaac atccgggggc
     1741 cgagttcctg atccatcccg aatgcggctg ctcgacccag gcgatggagt acgtggcgac
     1801 cggtgacatc gaccccgaag gcgtgcacat gctctcgacc tcgggcatga tcgagcacgt
     1861 ccgcgagaac cccgaaggcg agtacatcgt cgccaccgag aacggcatgc tctacccgat
     1921 gaagcaggcg gtgccggggg ccaggctgat cgaggccaac cgcatggcct actgcaagta
     1981 catgaagatg atcacgctgc cgaagctgcg cgactcgctg cgcgacatga agttcgaagt
     2041 caaggtcgac gaggcgctgg ccgaaaaggc gcgcctgccg atcgagcgca tggtcagta
//
LOCUS       DAOQMF010000330         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35380_length_2099_cov_2.357632, whole genome shotgun sequence.
ACCESSION   DAOQMF010000330 DAOQMF010000000
VERSION     DAOQMF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35380_length_2099_cov_2.357632"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1075)
                     /locus_tag="PLV77_06200"
     CDS             complement(68..1075)
                     /locus_tag="PLV77_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HNC15473.1"
                     /translation="MRVWFDCTAAAHPVVLRPVIERFQARGDEILVTARENGETVGNL
                     ERLGIPHLVIGRHGGAGRLGKALALGSRSARLARPVWKFRPDLAVAHGSVDLAVLSQT
                     FFIPSAQMQDYEFAGLQRKIAWRVAKRVLVPDSIPVERLVKAGARENKLVRFPGLKED
                     YYLAGFEPDPTVPEALGIDLERVLVIVRPPPETAAYHADNPLYEKVIDRLAGADGVSA
                     VIIPRTEGQRERARARNSTSLIVPDRAVDAQSLIAYADLVVSAGGTMNREAVALGTPV
                     YTTFAGRMGGVDEALIADGSLKLLESAEQLELVKRKDQTGPKKPRDPDFLVQGMLEAL
                     A"
     gene            complement(1122..>2099)
                     /gene="glyA"
                     /locus_tag="PLV77_06205"
     CDS             complement(1122..>2099)
                     /gene="glyA"
                     /locus_tag="PLV77_06205"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNC15474.1"
                     /translation="VQPHSGAQANNAAYMALLEPGETIMGLALDHGGHLSHGMKLNVS
                     GKLYNVVPYHVSREDLRVDMDEVAKLAEENKPSLIVAGWSAYPRQLDFAAFREIADSV
                     GAKLMVDMAHFAGLVAAGEHPNPVPYADVVTTTIHKTLGGPRSGMILCTEELKADVNR
                     AVFPGQQGGPLMHVIAAKAVALGIASTDVFAERQRRTVENARALAAGLIDGGVDVLTD
                     GTDVHLVLVDLTSTGLDGQTGEDRLDQVGITVNRNAIPFDERPPMNPSGLRIGTPALS
                     TRGFTPEDMTEIARIISTALGPDFESERDSLAASSQALMDKYPLYPGLV"
BASE COUNT          336 a          686 c          717 g          360 t
ORIGIN      
        1 ccaaggcttt gggttgaagt tatccgcaca tggagcgaat aacttcaacc caggtgccga
       61 cctgctccta ggcgagggct tcgagcattc cctggacgag gaagtcaggg tctctcggct
      121 tcttcgggcc ggtttggtct tttcgcttga ccagttcgag ttgttcggcg ctttcgagaa
      181 gcttcaggct gccgtcggcg atcagggcct cgtcgacgcc gcccatcctt ccggcgaagg
      241 tcgtatagac cggggtgccg agggcgacgg cctcgcggtt catggtgccg ccggcgctga
      301 ccaccaggtc ggcgtaggca atcaggctct gggcatcgac tgcccggtcg ggaacgatca
      361 ggctggtcga attcctggcc ctggcccgct cccgctggcc ttcggtgcgg ggaatgatta
      421 ccgcgctcac cccgtccgcg cccgccaggc ggtcgatcac cttctcgtag aggggattgt
      481 ccgcgtggta ggcggcagtc tcgggcggcg gccgcacgat cactaggacc cgctcaaggt
      541 cgatcccgag cgcttccggg acggtcggat cgggttcgaa accggccagg taatagtcct
      601 ccttgaggcc gggaaaacgg accagcttgt tctcccgggc tcccgccttg accaggcgct
      661 cgaccgggat cgaatccgga accaggaccc gcttcgccac ccgccaggcg atcttccgct
      721 gcaggccggc gaactcgtag tcctgcatct gggccgaggg aatgaagaaa gtctgcgaga
      781 ggacggccag atccacggaa ccgtgggcca cggccaggtc gggccggaac ttccagaccg
      841 gccgggccag ccgggccgac cgcgaaccaa gggccagggc cttgccgagc ctgccggcgc
      901 cgccatgcct gccgatcacc agatgcggaa tgccgagccg ctcgaggttg ccgaccgtct
      961 ccccgttctc acgggcggtg accaggatct cgtcaccgcg agcctggaaa cgttcgatca
     1021 ccggccggag caccacgggg tgggcggcgg cggtgcagtc gaaccagacc ctcatcggtg
     1081 gatccccatc ggcttcggtc gccaggggca gtcgctccgg atcagaccag ccccgggtac
     1141 agggggtact tgtccatcag cgcctgtgaa cttgccgcca gcgaatcccg ctccgactcg
     1201 aagtcggggc cgagcgcggt gctgatgatc cgggcgatct cggtcatgtc ctccggcgtg
     1261 aagccgcggg tcgagagggc cggagttccg atcctgaggc cggaaggatt catcggcggc
     1321 cgctcgtcaa aggggatggc gttgcggttg accgtgatcc cgacctggtc gaggcgatcc
     1381 tccccggtct ggccgtcgag cccggtcgag gtcaggtcga ccagcacgag gtgaacgtcg
     1441 gtgccgtcgg tcaggacgtc gaccccgccg tcgatcagcc cggcggcaag cgccctggcg
     1501 ttctcgacgg tgcgacgctg gcgctcggcg aagacgtcgg tcgaggcgat gcccagagcc
     1561 acggccttcg ccgcgatcac atgcatcagc gggccgccct gctggccggg gaatacggcg
     1621 cggttcacgt cggccttgag ttcttcggtg cagaggatca tgccgctgcg ggggccgccg
     1681 agagtcttgt gaatcgtggt cgtgaccacg tcggcgtacg gcaccggatt cggatgctca
     1741 ccggccgcga cgaggccggc gaaatgtgcc atgtccacca tcagcttcgc cccgaccgaa
     1801 tcggcgattt cacggaaggc ggcaaagtcg agctgacgcg gataggccga ccagccggca
     1861 acgatcaggc tcggcttgtt ctcctcggcc agcttcgcga cctcgtccat gtcaaccctc
     1921 aggtcctcac gggacacgtg gtacggaacc acgttgtaga gcttgccgga gacgttcagc
     1981 ttcatcccgt ggctgagatg gccaccgtgg tcgagggcga ggcccatgat ggtttcaccc
     2041 ggctccagca gggccatgta ggcagcgttg ttggcctggg cgcccgaatg cggctggac
//
LOCUS       DAOQMF010000331         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35406_length_2098_cov_2.488497, whole genome shotgun sequence.
ACCESSION   DAOQMF010000331 DAOQMF010000000
VERSION     DAOQMF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35406_length_2098_cov_2.488497"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLV77_06210"
     CDS             <1..481
                     /locus_tag="PLV77_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15475.1"
                     /translation="DAAFPSPSPKGNMRKPFALGVITLAVTLSLGLAACGSSSDDSLS
                     NDELITQADQICTDYNDKLVTLTDNAGLDNNSSKQEVVAFISDEIVPLYENQVEELRA
                     LEPNEDDADAYNDIVTTLDTELQAVKDDPAAAMKMEDPFPEATAKAKDFGLTVCGAS"
     gene            524..1294
                     /locus_tag="PLV77_06215"
     CDS             524..1294
                     /locus_tag="PLV77_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNC15476.1"
                     /translation="MKTGGPFSYPAGMYLLFLDESGIPGEDGIFALGGVAIRADQWAE
                     VKDRFETCLEEAGWPADKEFKWSEISRSTAAADAAYPVYDCLATLPVHCLVTVLYTEG
                     DDPVNREKFFANPDLIYRTAFTFVTERYQRFLAHHDSHGVIVLDSRQYETDDRLRRYF
                     DRIHNEGTDFAELDRIVDGLLLGPSHFSLGLQLADLVVGPTRTAQFSGPGTARHCFKT
                     LKPTFMTHPASGALEGVGLKVFPHQETTREDDRLFRPA"
     gene            1394..>2098
                     /locus_tag="PLV77_06220"
     CDS             1394..>2098
                     /locus_tag="PLV77_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNC15477.1"
                     /translation="MLDENRLAVLNAVCDTIVPSLERDDDPTGFWARSAADTKANEVV
                     AQTIGEMPVADQEGMGLLLDSLALQNFPSLSQASREQILVNTSLASREAAVGVAALTQ
                     MTLFFHYGLPPNPAWEQFGFPGPASPPPQVEKTIHPLTPGDGDVLEADAVIVGSGAGG
                     GVIAARLAEAGMKVIVLEMGGYFNESDFDQTELNGFARMYWRGGPTYSADFNISIQAG
                     SCLGGGTLINWTNSLKP"
BASE COUNT          380 a          752 c          637 g          329 t
ORIGIN      
        1 cgatgcggca ttcccgtccc cctccccgaa aggaaacatg agaaagccct tcgccctcgg
       61 cgtgatcacc ctcgccgtaa cgctttcgct cggcctcgcc gcctgtggct ccagcagcga
      121 cgactcgctc tctaatgacg agctgatcac tcaggccgac cagatctgca ccgattacaa
      181 cgacaagctg gtcaccctga ccgacaatgc aggccttgac aacaactcga gcaagcagga
      241 agtcgtcgcc ttcatcagtg acgaaatcgt cccgctctac gagaaccagg ttgaagagct
      301 gcgtgccctc gagcccaacg aggatgacgc tgacgcctac aacgacatcg tcaccacgct
      361 ggacaccgag ctccaggcgg tcaaggacga tcccgccgct gcgatgaaga tggaagatcc
      421 cttccccgag gcgacggcca aggccaagga cttcgggctg accgtctgcg gcgctagctg
      481 atccgttccc gaacggaacc gatgcgttca aaaggggccg tccgtgaaaa cgggcggccc
      541 cttttcctat cctgccggca tgtacctgct cttcctcgac gagtccggca tccccggtga
      601 ggacggaatc ttcgccctcg gcggggtcgc gatccgggcc gaccagtggg ccgaggtcaa
      661 ggaccgtttc gagacctgcc tggaagaagc gggctggccg gccgacaagg agttcaaatg
      721 gtcggagatc agccgcagca cggcggcggc cgacgcggcc tacccggtct acgactgcct
      781 cgcgacgctg ccggtccact gcctcgtgac cgtgctctac accgaaggcg atgacccggt
      841 caaccgggag aagttcttcg ccaacccgga cctgatctac cggaccgcct tcaccttcgt
      901 gaccgagcgc taccagcgct tcctcgccca ccacgattcc cacggcgtga tcgtgctcga
      961 ctcccggcag tacgagacgg acgaccgcct gcgccggtac ttcgaccgca tccacaacga
     1021 gggcaccgac ttcgccgagc tcgaccgcat cgtcgacggc ctgctgctcg ggccttccca
     1081 cttttcgctc ggcctccagc tcgccgacct ggtcgtcggg ccgacccgga ccgcgcagtt
     1141 cagcggcccc ggcaccgcca ggcactgctt caagacgctg aagccgacct tcatgaccca
     1201 tccggcgagc ggcgccctcg aaggtgtcgg cctcaaggtg tttccccatc aggagacgac
     1261 ccgcgaggac gaccggctct tccggccggc ctgacccgcg cggcgaatca cccccatcgg
     1321 atgagcgggg cgccggcttt gcggcgctag actgatccgg ccggagagga accgaggaga
     1381 gaggagcacg agagtgcttg acgagaaccg ccttgccgtc ctgaatgccg tctgcgacac
     1441 gatcgtgccg tcgcttgagc gggacgatga cccgaccggg ttctgggccc ggtccgccgc
     1501 cgacaccaaa gcaaacgagg tcgtcgcgca gacgatcggc gagatgcccg tggcagacca
     1561 ggagggcatg ggtctcttgc tcgacagcct ggcgctgcag aacttccctt cgctctcgca
     1621 ggcctcgcgc gagcagatcc tcgtcaacac ctcgctggcc agccgcgaag cggcggtcgg
     1681 ggtcgcggcg ctgacccaga tgaccctctt cttccactac ggcctgccgc cgaatccggc
     1741 ctgggagcag ttcggcttcc ccggcccggc ctctccccct ccccaggtgg aaaagacgat
     1801 ccacccgctc accccgggtg acggcgacgt gctcgaagcc gacgccgtga tcgtcggctc
     1861 cggcgccggt ggcggtgtga tcgccgcccg gctggccgag gccggcatga aggtgatcgt
     1921 gctcgagatg ggcggctatt tcaacgagtc cgacttcgac cagaccgagc tgaacgggtt
     1981 cgcgcggatg tactggcgcg gcggcccgac ctactccgcc gatttcaaca tcagcatcca
     2041 ggccggaagc tgcctcggcg gcggcaccct gatcaactgg accaacagcc tcaagccg
//
LOCUS       DAOQMF010000332         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35570_length_2092_cov_4.272459, whole genome shotgun sequence.
ACCESSION   DAOQMF010000332 DAOQMF010000000
VERSION     DAOQMF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35570_length_2092_cov_4.272459"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PLV77_06225"
     CDS             <1..1395
                     /locus_tag="PLV77_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNC15478.1"
                     /translation="TGAEADDLRKAIGKKKREMMAAMKPKFMEGLKASGTDHALATYL
                     WSINEAAADYSFNKSHAACYALISYRTAYLKANYPAEYMAAVISSVMSTKDKVPFFVN
                     HCSSMGIRVLPPDVNISDHSFVVDEGNIRFGLDAVKNVGYSAVDAIINARNDRPFESL
                     WDFCERVDNRTVNKRAVECLIKCGAFDLLPGSRQGMLEKLPDAAAAGAKAQSDLMLGQ
                     ESIFSLGGADEGAGGGSHEPISEVEFDQKELLALEKETMGTFLSSHPLDGLKEAILAE
                     ADCSLGDLRLQEDGSRVKVGGLITAYKKVRTRAGKNIAFATLSDIEHDIELMLFNLED
                     SEKQQLVQMDEVVMVRGTVDQSEEGSKLKVRDAELFNPSEAQIEKAKIAYAKKIEPFV
                     VRVVPEQMSLDTLEDMKSVFGQHQGESDVVVVVEGDREHRLKLGPDFRVRHSPGLATE
                     IDQIFGDGTAARAA"
     gene            1537..>2092
                     /locus_tag="PLV77_06230"
     CDS             1537..>2092
                     /locus_tag="PLV77_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC15479.1"
                     /translation="MTATETSEETTVDESSEPKFSLELNQDQKDIQEWVHGFAENVMR
                     PAAHEWDEREEFPWEIVQEAAKIGLYGFEGTAQFFADPTGLSLPIVNEELFWGDAGLG
                     MAIQGTGLAVASIFGQGTPEQMGEWVPQCYGTEDDVKIAAFCSSEPDAGSDVSNVRTT
                     AKYDEAKDEWVINGQKAWATNGGIA"
BASE COUNT          434 a          641 c          672 g          345 t
ORIGIN      
        1 accggcgcgg aggccgacga cctgcgcaag gcgatcggca agaagaagcg cgagatgatg
       61 gccgcgatga agccgaagtt catggagggg ctgaaggctt ccggcaccga ccatgccctc
      121 gccacctacc tctggagcat caacgaagcc gccgccgact actccttcaa caagtcccat
      181 gctgcctgtt acgcgctgat ctcctaccgc accgcgtacc tgaaggcgaa ctacccggcc
      241 gagtacatgg ccgccgtgat ctcctcggtg atgagcacca aggacaaggt gccgttcttc
      301 gtcaaccact gctcctcgat gggcatccgg gtgctgccgc cggacgtgaa catctccgac
      361 cacagcttcg tggtcgacga gggcaacatt cgtttcggcc tcgacgcggt caagaatgtc
      421 ggctactcgg cggttgacgc gatcatcaat gcccgcaatg accggccctt cgaatcgctt
      481 tgggatttct gcgaacgggt cgacaaccgc acggtcaaca aacgggcggt cgaatgcctg
      541 atcaagtgcg gcgccttcga cctgctgccc ggctcccgcc agggcatgct ggagaagctg
      601 cccgacgcgg cggcggccgg cgccaaggcc cagtccgacc tgatgctggg gcaggaatcg
      661 attttcagtc tgggaggggc cgacgaagga gcaggcggag gcagtcacga gccgatcagc
      721 gaggtcgagt tcgaccagaa ggagctgctc gccctggaaa aggaaacgat gggcaccttc
      781 ctttcatccc atccgctcga cgggctcaaa gaggcgatcc tggctgaagc ggactgcagc
      841 ctcggcgatc tgaggcttca ggaggacggc tcccgggtca aggtaggtgg cctgatcacc
      901 gcctacaaga aggtgaggac ccgggccggc aagaacatcg ccttcgccac cctcagcgac
      961 atcgagcacg acatcgaact gatgctcttc aacctggagg attccgagaa gcagcaactg
     1021 gtccagatgg acgaggtcgt gatggtcagg gggaccgtcg accagtccga ggagggctcg
     1081 aagctgaagg tgcgcgacgc cgagcttttc aatcccagcg aggcccagat cgagaaggcg
     1141 aagatcgcct acgcaaagaa gatcgagccc ttcgtggtcc gggtggtgcc ggagcagatg
     1201 tcgctggaca cgctggagga catgaaatcg gttttcggcc agcatcaggg tgaatcggat
     1261 gtggtcgtgg tggtcgaagg ggaccgggag caccggctca agctcggtcc cgacttccgg
     1321 gtcaggcatt cccccgggct ggcgaccgag atcgaccaga tcttcggtga cggcaccgcg
     1381 gcccgcgctg cctgaacgac attccggcgg accctttgcc gacagagccc cttctcgcca
     1441 tcatttgagc taggagaagg ggcgggtttc cgaggtcgtc ccgcgaagac gggacgacat
     1501 gcgaatgatt acaccgatcc gtgtaacatt cggggtatga ctgctacaga aacttcagaa
     1561 gaaaccactg ttgatgagtc ttctgagccc aagttcagcc ttgagctgaa tcaggaccag
     1621 aaggacattc aggagtgggt ccacggcttt gccgagaatg tgatgcgccc cgccgcccac
     1681 gaatgggacg agcgcgagga gttcccctgg gagatcgtgc aggaggccgc caagatcggc
     1741 ctctacggct tcgagggcac cgcacagttc ttcgccgacc cgaccggcct cagcctgccg
     1801 atcgtcaacg aggaactgtt ctggggcgat gccggtctcg gcatggcgat ccagggcacc
     1861 ggccttgccg tcgcctcgat ctttggccag ggcaccccgg aacagatggg tgaatgggtt
     1921 ccccagtgct acggcaccga ggatgacgtc aagatcgccg cgttctgctc ctccgagccc
     1981 gacgccggct cggatgtctc caacgtccgg accacggcca agtacgacga ggccaaggac
     2041 gaatgggtga tcaacggcca gaaggcctgg gcgaccaacg gcggcatcgc ca
//
LOCUS       DAOQMF010000333         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35799_length_2086_cov_2.651403, whole genome shotgun sequence.
ACCESSION   DAOQMF010000333 DAOQMF010000000
VERSION     DAOQMF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35799_length_2086_cov_2.651403"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLV77_06235"
     CDS             <1..612
                     /locus_tag="PLV77_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15480.1"
                     /translation="AWILSVVLAAGAIGVAFGVVNWKVGCMVAGYGVIQVAYSFGLKQ
                     VVIIDVMTLATLFILRVMAGAEAVDAHASEWLILCTGMLACFLGFTKRRQEAVSELHE
                     GTSSRPVLEHYSLPFLDQMVAMVTTGTVVSYAIYTVNSPLAGSEMMLTIPPVVYGIFR
                     YLYLIYDRSDDRSTAAIVAEDRGVIIAGAVFVAVALLVLYAFD"
     gene            1060..>2086
                     /locus_tag="PLV77_06240"
     CDS             1060..>2086
                     /locus_tag="PLV77_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15481.1"
                     /translation="MNFPARIIARVVIVAAILGLGFASTAQADRSFSVRYTTNDTGSI
                     KGIANTNMTCPSSASGCSTAQTQDPTSSANSYYNNNYDMTYVDVDSDSSTFNSSTANQ
                     SLPSGSTILFAGLYWGGDYSTGSASAPNSSDRNKVKLKLPGSSTYTTITASTLDDSTL
                     NVGRYQAFADVTSLIQALPNAGNGTYGVANIQAGKGSDHYAGWTLIVAYRNTAETAKN
                     MSIFDGLVTIRPSDPPTTIPFSGFLTPPSGPVNPQIGFVTWEGDHGLVGDSASLNGNT
                     LSDAQHPATNFFDSRISYNGVLYTDRNPNYANALGIDAAWTTPPPGTVGNSETSATLR
                     VTTSGDQY"
BASE COUNT          405 a          720 c          575 g          386 t
ORIGIN      
        1 gcctggattc tctcggtggt gctcgccgcg ggtgcgatcg gggtcgcctt cggggtggtc
       61 aactggaagg tcggctgcat ggtcgccggt tacggcgtga tccaggtcgc ctacagcttc
      121 ggcctcaaac aggtcgtgat catcgatgtg atgaccctcg ccacgttgtt catcctccgg
      181 gtgatggccg gcgccgaggc cgtggatgcc cacgcctcgg agtggctgat cctctgcacc
      241 ggcatgctgg cctgcttcct cggcttcacc aagcggcgcc aggaagcggt atccgaactt
      301 cacgagggaa caagcagccg accggtgcta gagcactact ccctgccctt cctcgaccag
      361 atggtcgcca tggtgactac cggcaccgtg gtcagttacg cgatctacac cgtgaactcg
      421 cccctggccg gttccgagat gatgctgacg attccccccg tcgtctacgg catcttccgc
      481 tacctctatc tgatttacga ccgctcggat gaccgttcaa ccgcggcgat cgtggccgag
      541 gaccggggag tgatcatcgc cggggcggtc ttcgttgcag tcgccctgct ggtcctctac
      601 gccttcgact gatccaggcc ggcgggcaga atccggtcgt ccgattcggg ccggaggtgc
      661 ctgacttgcg gtcgaccggc tcaagccggc aaagcaggca aatcccggct atttgcgggc
      721 acattcaagg cagtccgccg agcccgataa ccggtaaacc cgtgctgtac cgccgattgc
      781 gggtaattcg catttttcat atcagtcggt ccggacactt gacacgcatc agaaaacgcg
      841 tattctcaat cgaccaataa gaacacggga gacgggtgcg gacccccttc gagagccggt
      901 tggaaaccgg cagggatcca gaaaagacac tctgtcaact gctgaatctc gatacttgcg
      961 cagttgaccg ggccttcgag gctttcccac cgttcagccg ggccaggaaa tggctcgaac
     1021 gaaaccggtg tacccaaaag agaaacctcg gagaactgaa tgaatttccc cgctcgaatc
     1081 atcgcccgcg tcgtcatcgt cgccgccatt ctcggcctcg gcttcgcttc cactgcccag
     1141 gctgaccgca gcttcagcgt tcgctacacg accaatgaca ccgggtcgat caagggcatc
     1201 gcgaacacca acatgacctg cccctcgagc gcatcgggct gttcgaccgc acagacccag
     1261 gaccccacct cctcggcgaa ctcgtactac aacaacaact acgacatgac ctatgtcgac
     1321 gttgattccg acagttcgac cttcaactcc tcgacggcta accagagcct gccttccgga
     1381 tcgacgattc tcttcgccgg tctctactgg ggcggcgatt acagcaccgg ctcggcgtcc
     1441 gcgcccaact cgagcgaccg caacaaggtc aagctcaagc ttcccggatc gagcacctac
     1501 accacgatca cggccagcac gctcgacgac tcgaccctga acgtcggccg ttaccaggcc
     1561 ttcgctgacg taaccagcct catccaggcg ctgcccaacg cgggcaacgg cacctatggc
     1621 gtggccaaca tccaggccgg caagggctcg gatcactacg ccggctggac cctgatcgtc
     1681 gcttaccgga acactgcgga aacggccaag aacatgtcga tcttcgacgg tctggtcacc
     1741 atccgtccct cggacccgcc gaccacgatt cctttctcgg gcttcctgac gccgccttcg
     1801 ggtccggtca acccccagat cggattcgtc acctgggagg gtgaccacgg cctggtcgga
     1861 gattcggctt ccctgaacgg caacacgctc tccgatgccc agcatccggc gaccaacttc
     1921 tttgattcgc ggatctcgta caacggcgtg ctctacaccg atcgcaaccc gaactacgca
     1981 aacgcgctcg gcatcgacgc cgcctggacc accccgcccc cgggcacggt cggcaacagc
     2041 gaaacctcgg cgaccctgcg ggtaaccacc tcgggtgacc agtacc
//
LOCUS       DAOQMF010000334         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35822_length_2085_cov_2.972414, whole genome shotgun sequence.
ACCESSION   DAOQMF010000334 DAOQMF010000000
VERSION     DAOQMF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35822_length_2085_cov_2.972414"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLV77_06245"
     CDS             complement(<1..474)
                     /locus_tag="PLV77_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNC15482.1"
                     /translation="MESLLAEYVADRSHESEPAPGSFTGAAGGAACGDLARISLVVEN
                     GVVSRTCFGTEGCAATRAACACVCELIEGESVLEAARIGATRIEEELGGLSPARRHAA
                     MLAGDALHRALSAVASSGELISPLVEGRVLVAVSGGVDSAVAALGEKEAGAEVVAV"
     gene            523..1497
                     /locus_tag="PLV77_06250"
     CDS             523..1497
                     /locus_tag="PLV77_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNC15483.1"
                     /translation="MSKGWNADDITDQTGRTAIVTGGNSGIGLITARELLRHGARVII
                     GCRNEDKGKAAIEQIASDLGTPGIATGIEVRRLDLASLDSVREFAESVDADLPDGLDL
                     LINNAGVMAPPRLLTEDNFELQFGTNHLGHFALTGRLFDTLKRKQGARIVTISSVAAT
                     MGKLDFDDLQSVEGYNRWRAYGQSKLANLVFAMDLQSLIRQAGLDMASIAAHPGVSNT
                     NLTAAGNDLDSGWLGLLSKPFLALSDLLIAQDAAHGALPTLFAATEPGLTGGEYIGPD
                     GLRQYRGSPTIVAPRRLALDSGTGDRLWKASTELTGVEYDFSPASPAA"
     gene            complement(1515..>2085)
                     /locus_tag="PLV77_06255"
     CDS             complement(1515..>2085)
                     /locus_tag="PLV77_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15484.1"
                     /translation="NKTDTLNLDGGWRQVTLSGTGISGPTGPTGATGSTGPTGSTGSS
                     GATGATGSTGATGATGPTGPKGPTGATGPKGPSGNNATPRVNRIHTVVRVGAGPVAMV
                     SVHCPKASCTIWQRQGRARSRGRVIQTRVIGPNRIAAGGTARFRVTVPPRIRNLLTRQ
                     RSGTVNVYLAVQADKGNSIRRNVRIGIRR"
BASE COUNT          336 a          754 c          659 g          336 t
ORIGIN      
        1 caccgcgacg acttcggccc cggcctcctt ctcgcccagt gcggccacgg cgctgtcaac
       61 gccacccgag accgcgacca gcaccctgcc ctcgaccagt ggtgaaatca gctcgcccga
      121 ggaagcgacg gccgaaagcg cccggtgaag cgcgtcgccg gccagcatcg ccgcgtgacg
      181 ccgggccggg ctgagcccgc cgagctcctc ctcgatccgg gtcgccccga tccgggccgc
      241 ctccagcacg ctttcaccct cgatcagctc acaaacgcag gcacaggcgg ccctggtcgc
      301 ggcgcaaccc tcggttccga aacaggtgcg cgaaaccacg ccgttttcga ccaccaggga
      361 aatccgggcg agatcaccgc aggcggcgcc gccggcggcc ccggtgaacg aaccgggggc
      421 cggttccgac tcatgcgagc ggtcggcgac atattcggcg agcagcgact ccatccccta
      481 gaggttaaat ggtcgattcc cccagcggat acgctgcccc gcatgagcaa aggctggaac
      541 gcagatgaca tcaccgatca gacgggtcgc accgcgatcg tcaccggggg caacagcggc
      601 atcgggctga tcaccgcccg tgaactgctg cggcacggag cgcgggtgat catcggctgc
      661 cgaaatgaag acaagggcaa ggccgcgatc gagcagatcg cctccgacct cggcacgcca
      721 ggtatcgcca ccgggatcga agtgcgacgg ctcgacctcg cctccctgga ttcggtccgc
      781 gagttcgccg agtctgtcga cgccgacctg cccgacggac ttgacctcct gatcaacaac
      841 gccggtgtca tggctccgcc ccggctgctc acggaagaca atttcgagct ccagttcggc
      901 accaaccacc tcggccactt cgccctgacc ggccggctgt ttgacacgct gaagcgcaaa
      961 caaggggcaa ggatcgtgac gatcagttcc gtggcggcca ccatgggcaa gctcgatttc
     1021 gatgacctgc agtcggtcga gggctacaac cgctggcggg cctacggcca gtcgaagctt
     1081 gccaatctgg tcttcgcgat ggacctgcag tcgctgatcc gccaggccgg tctcgatatg
     1141 gcgagcatcg ccgcccaccc cggtgtctcg aacaccaacc tgaccgcggc cggcaatgac
     1201 ctcgactcgg gctggctcgg acttctctca aagccgttcc tcgctctttc cgatctgctg
     1261 atcgcccagg atgccgcaca cggcgccctg cccaccctct ttgccgcgac cgagcccggt
     1321 ttgacaggcg gcgagtacat cggcccggac ggtctgcgcc agtaccgcgg ttcacccacc
     1381 atcgtggccc cgcgcaggct cgcgctcgat tccgggacag gcgatcgcct gtggaaggcg
     1441 tcaaccgaac tgaccggggt cgaatacgac ttctccccgg ccagtccggc tgcctgaatc
     1501 ccggcctctc gctgctaccg cctgattccg atccggacgt tcctccggat cgagttgccc
     1561 ttgtcggcct gtaccgccag gtagacgttg acggttcccg accgctggcg ggtcagcagg
     1621 ttccggatcc ggggcggaac cgtgacccgg aaacgggccg tgccacctgc ggcgatccgg
     1681 ttcggcccga ttaccctggt ctgaatgacg cggccgcggg aacgggcccg gccctgtcgc
     1741 tgccagatcg tgcaggaggc cttcggacaa tggacgctga ccatcgctac cggaccggcg
     1801 ccgacccgga cgaccgtgtg aatacggttc acccggggag tggcgttgtt gccacttggg
     1861 cccttggggc cggtggctcc ggttggccct ttcggcccgg tcggccccgt cgcgccggtt
     1921 gctccggtcg aacccgtggc gccagtcgcg cccgaactgc cggttgagcc agtaggcccg
     1981 gttgagccgg tcgcgccagt aggcccggtt ggcccgctga tcccggtccc gctcagggtg
     2041 acctgccgcc agccgccgtc caggttgagt gtgtcggtct tgttt
//
LOCUS       DAOQMF010000335         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_35880_length_2083_cov_3.596647, whole genome shotgun sequence.
ACCESSION   DAOQMF010000335 DAOQMF010000000
VERSION     DAOQMF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35880_length_2083_cov_3.596647"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1165)
                     /gene="proB"
                     /locus_tag="PLV77_06260"
     CDS             complement(77..1165)
                     /gene="proB"
                     /locus_tag="PLV77_06260"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNC15485.1"
                     /translation="MTLVVKLGSSIVSADDGSLREEVLDSVCAQAAALRRAGEPLVMV
                     TSGSIARGIRLLGLQARPRAIDELQAASAVGQGSLFREYESRLETGEAAAAQVLLTAS
                     DVANRTSYVNARQTLRRLLDWGVVPVINENDTTATDEITFGDNDFLAAQVAIMLEARL
                     LVLLTNTDGVFTADPAHDPEARLIDELSSPADLEALEIGDRTSAFGTGGMRSKVAAAG
                     MASGSGIEVRICNGTVDGTLARAAAGEAAGTTFRALKSDAPAFKQWLRYAKPVAGRIV
                     VDEGAARVLRKSGSSLLPVGVTSIEGAFEAGDAVEVVASGTVIGKGIANYSAGEADRI
                     KGMKSVEVLELMPHAAEELIHRDYFVLS"
     gene            complement(1291..>2083)
                     /gene="cgtA"
                     /locus_tag="PLV77_06265"
     CDS             complement(1291..>2083)
                     /gene="cgtA"
                     /locus_tag="PLV77_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HNC15486.1"
                     /translation="KSSLLSRLTRADPKVADYPFTTLEPVLGTIETPDRQVVIADIPG
                     LIEGAAEGAGLGHEFLAHVERCAMLIHVVELAPSDGEPLENYRAVRAELAAHSTGLEK
                     LPELLVLSKSDLWTEADIADQVEEWDRELGESVLGIIPVSSATGAGLERLKESIVRFV
                     PDQVEAPVVTSADGIEVDFEAEHLTYRPKGEGGYSVEEEEEGAFRIHGHGIEVLFERF
                     DTGNEEALAYLESRLTEIGVMAELKRAGFESGDEIRVGDLEFELY"
BASE COUNT          348 a          704 c          674 g          357 t
ORIGIN      
        1 gtctcacgct aggccgtccc gcctgagaca gagccccttc cttctaaccc tgaatgtgag
       61 aggaagggac ttgcagttaa gaaaggacga agtaatcgcg gtggatcagc tcttccgcgg
      121 cgtggggcat cagttcgagc acctcgacgc tcttcatgcc cttgatccgg tcggcctcgc
      181 cggccgagta gttggcgatg cccttgccga tcaccgttcc ggacgcaacc acctcaaccg
      241 cgtccccggc ctcgaaagca ccttcgatcg aggtcacacc gaccggaagc aggcttgacc
      301 cgctcttgcg gagcacgcgg gcggcgcctt cgtcgacgac gatcctgccg gccaccggct
      361 tggcgtacct gagccactgc ttgaaagccg gcgcgtcgct cttcagcgcg cggaaggtgg
      421 tgcccgccgc ctcgccggcg gcagcgcgcg caagggttcc gtcaacggtg ccgttgcaga
      481 tccttacttc gatcccggag ccgctggcca tcccggccgc ggcgaccttg ctgcgcattc
      541 cgccagtgcc gaaagccgag gtgcggtcgc cgatctccag cgcttcaagg tcggccgggc
      601 ttgacaattc gtcgatcagc ctggcctcgg ggtcatgggc cggatcggcg gtgaagacgc
      661 cgtcggtatt ggtcaagaga accagcaacc tggcctcaag catgatcgcc acctgggcgg
      721 cgaggaagtc gttgtccccg aaggtgatct cgtcggtggc ggtggtgtcg ttctcgttta
      781 tgaccgggac cacaccccag tcgagcagcc ggcgcagggt ctggcgggcg ttcacgtaac
      841 tggtccggtt ggcaacgtcg gatgcggtca gcagcacctg ggcggccgct gcctcccccg
      901 tctcgagccg ggactcgtac tcgcggaaga gcgagccctg gccgaccgcg gaagcggcct
      961 gcagctcgtc gatcgcccgc ggcctggcct ggagaccgag cagccggatc ccccgggcga
     1021 tcgacccgga ggtgaccatg accagcggct ccccggcccg ccgcagagca gccgcctggg
     1081 cgcagacgga atcgagcacc tcctcgcgca ggctcccgtc atccgccgaa acgatcgaag
     1141 atccaagttt caccaccaaa gtcatctccc caccaaccct agaggctgat gccacgaccg
     1201 gcagagcccc tcccaaccat cctttgctcc tgatgggagg ggcgggtgga gattggaggg
     1261 ccggcgaaga ccggcccgac tgcagttaag tcagtaaagc tcgaactcga ggtctccgac
     1321 ccggatctcg tctccggact caaacccggc ccgtttcagt tcggccatca cgccgatttc
     1381 ggtgagacgg gattcgaggt aggcgagtgc ttcctcgttg ccggtgtcga agcgttcgaa
     1441 gagaacctcg atgccgtggc cgtggatccg gaaggcacct tcttcttcct cctcgaccga
     1501 gtagccgccc tcccccttcg gccggtaggt gaggtgttcg gcttcgaagt cgacttcgat
     1561 cccgtcggcc gaggttacga ccggcgcctc gacttggtca ggcacgaagc ggacgatcga
     1621 ctccttcagc cgctccaggc cggcgccggt cgcagatgaa acgggaatga tccccagcac
     1681 ggattcgccg agctcgcgat cccattcctc gacctggtcc gcgatgtcgg cctcggtcca
     1741 gaggtcggac ttcgaaagca ccagcagctc gggcagcttc tccagaccgg tgctgtgagc
     1801 ggcaagctcg gcccgtaccg cccggtagtt ctccagcggc tcaccgtcgg agggggcaag
     1861 ctcgacgacg tggatcagca tcgcgcagcg ttcgacgtgg gcgaggaact cgtgaccgag
     1921 cccggccccc tcggcggcac cctcgatcag tcccgggatg tcggcgatca cgacctggcg
     1981 gtccggggtt tcgatcgttc cgagaaccgg ctcgagcgtg gtgaacgggt agtcggcaac
     2041 cttggggtcg gccctggtca ggcgggagag cagcgagctc ttg
//
LOCUS       DAOQMF010000336         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_36007_length_2079_cov_4.361166, whole genome shotgun sequence.
ACCESSION   DAOQMF010000336 DAOQMF010000000
VERSION     DAOQMF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36007_length_2079_cov_4.361166"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLV77_06270"
     CDS             <1..544
                     /locus_tag="PLV77_06270"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC15487.1"
                     /translation="GLVALAVGVILFGTFAGGLVKLDLGAINKQTSGRANLVEGGMDL
                     FEARPLYGYGPGSFSVAFKREVAGPNAPVTESHTEPVTVAAEQGLIGLAFYLALLASI
                     LAALLAGMRKVMPGLGAPPRSYDPDRGPPAARAAILAAFVAVLVHTLTYAGFLDDPVT
                     WVLIAIGYSLAFPCRATSAA"
     gene            520..>2079
                     /locus_tag="PLV77_06275"
     CDS             520..>2079
                     /locus_tag="PLV77_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNC15488.1"
                     /translation="MSGYLRRLATTGAAYTAASIISKLIAVALLPLYTRYLTPADYGA
                     AEVMFSAVVAASIIIRLGVVEALLRFYYKQGEEPAAVVSTSFATLFWAGLISVIVLMP
                     FAGPISQLLLGESEPTLGRIAIVGLFVLTLSEYLLTIFRLDERARAFFTVTMLSVLIT
                     IPVTVILVVPLDLGAEGLLAGSYGTGLAIVLGLIFYNRRRLALAPDRPLLKRMMAFGL
                     PTMPAELSLYSLSFIDRILIARKLGLAEAGLYSIAIKMSQGIAVLVRGFQLAFPPLAY
                     SIQDDDEARLAYSAVVTWFVTVMAFVVSFLWLIAPWIVGLLAAPEFSEAAVVVGPVSA
                     GAALYALYMVLLVVLGRTGRTEYNLPATIAGLVVNVGLNLLLLPLWGIIGAGVALVVS
                     YLVVVVLMYLFTQRLFPVPYEWLRLVRVLLATAALVAIGEVFVPDDGLAWLAVRLLLV
                     LALPFALLLTGFFTAEERGWLLLLTKPGELKAKILAAREVGKAAGEEGEGTSASGRPG
                     SRGLPEAVEVEQ"
BASE COUNT          252 a          745 c          666 g          416 t
ORIGIN      
        1 cggactcgtc gcgctggccg tcggggtgat cctcttcggc accttcgccg gcggcctggt
       61 caagctcgac ctcggcgcga tcaacaagca gaccagcggc cgggcgaacc tggtcgaggg
      121 cggaatggac ctcttcgagg cgaggccgct ctatggctac gggcccggtt ccttctcggt
      181 tgccttcaag cgggaggtgg ccggaccgaa cgcgccggtg accgaatccc acaccgaacc
      241 ggtcaccgtc gcggccgagc aggggctgat cggcctggcc ttctaccttg cgctgctggc
      301 cagcatcctt gccgcgctgc tggccggaat gcgaaaggtg atgcccggcc tcggtgcccc
      361 gccgcgctcg tacgacccgg accgcggacc ccccgccgcc agggccgcca tcctcgccgc
      421 cttcgtcgcg gtgctggttc acacgctcac gtacgcagga tttctcgacg acccggttac
      481 ctgggtcctg atcgccatcg gctatagcct cgcctttcca tgtcgggcta cctccgccgc
      541 ctagcgacaa ccggcgccgc ctacacggcg gccagcatca tctcgaagct gatcgcggta
      601 gccctgttgc cgctttacac gcgctacctg accccggctg attacggcgc cgcggaagtc
      661 atgttctcgg ccgtggtcgc ggcctcgatc atcattcggc tcggcgtggt cgaggccctg
      721 ctccgcttct attacaagca gggtgaggag ccggccgccg tcgtctcgac ttccttcgcc
      781 accctgttct gggcggggct gatctcggtc atcgtcctga tgcctttcgc cgggccgatc
      841 tctcagctcc tcctcgggga atccgaaccg accctgggcc ggatcgcgat cgtcggcctc
      901 ttcgtgctca ccctttccga ataccttctg accatcttcc ggctggacga gcgggcccgg
      961 gccttcttca cggtgaccat gctctcggtc ctgatcacga tcccggtcac cgtgatcctg
     1021 gtcgtcccgc tcgacctcgg ggccgaaggc ctcctggccg gcagctacgg caccggcctc
     1081 gcgatcgtcc tcggcctgat cttctacaac cgccgtcggc tcgccctggc ccctgaccgt
     1141 ccgctgctca agcggatgat ggccttcggc ctgccgacca tgccggccga gctctcgctc
     1201 tactcgctga gcttcatcga ccgcatcctg atcgcccgca agctcggtct ggccgaggcc
     1261 gggctttact cgatcgcgat caagatgtca caggggatcg cggtcctggt ccgcggtttc
     1321 cagctcgcct tcccgccgct cgcctactcg atccaggatg acgacgaggc ccgcctcgcc
     1381 tactcggcgg tggtcacctg gttcgtcacg gtgatggcct tcgtggtcag cttcctctgg
     1441 ctgatcgctc cctggatcgt cggcctgctc gccgcccccg agttctccga ggcggccgtc
     1501 gtggtcggac cggtatcggc cggcgccgcc ctctacgcgc tctacatggt gctgctggtc
     1561 gtgctcggcc ggaccgggcg caccgagtac aacctgccgg cgacgatcgc cgggctggtc
     1621 gtcaatgtcg gcctcaacct gctgctgctt ccgctctggg ggatcatcgg ggccggggtc
     1681 gcgctggtcg tctcctacct ggtcgtggtg gtgctgatgt acctcttcac ccagcgtctc
     1741 ttcccggtcc cctacgagtg gctgcggctg gtccgggtgc tgctcgccac cgcggccctg
     1801 gtcgcgatcg gcgaggtctt cgtcccggat gacggtctcg cctggctggc ggtgcggctg
     1861 ctgcttgttc tcgcgctccc gttcgctctg ctcctcacgg gctttttcac cgcggaggag
     1921 cgggggtggt tgctcttgtt gacgaagccc ggtgagctga aggcgaagat cctcgccgca
     1981 cgggaggttg gaaaagcggc gggcgaggag ggtgaaggca ccagcgcctc aggcaggccc
     2041 gggagtcgcg ggttgccgga ggcggttgag gtcgaacag
//
LOCUS       DAOQMF010000337         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_36093_length_2077_cov_2.524233, whole genome shotgun sequence.
ACCESSION   DAOQMF010000337 DAOQMF010000000
VERSION     DAOQMF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36093_length_2077_cov_2.524233"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..985)
                     /locus_tag="PLV77_06280"
     CDS             complement(59..985)
                     /locus_tag="PLV77_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15489.1"
                     /translation="MPTDLKSAVLLIVFNRPGTTKAVLEAIREVKPPRMYIASDGPRP
                     DRRGEEIEVENVRSLVAEGIDWDCEVKTLFRKENLGCRLAVSGAISWFFEQEEEGIIL
                     EDDCLPDRSFFFFCEDLLDRYRGDKRVMHVAGSCLVKDLPAEQSYFFSKYPAVWGWAT
                     WRDAWSDYSLTTNEFESVFEAFAGQFQTAEEEKYWRKTLKNYFDGGIDTWDYPWAFSI
                     WRCQGLSIYPTKNLVRNIGFSSGATHTQAWKDYRGLGDAALDSMDSIVHPPVVEINQE
                     LDRRVFNESYRQPPKIVIGLKLLQRLVKNSVR"
     gene            1176..>2077
                     /locus_tag="PLV77_06285"
     CDS             1176..>2077
                     /locus_tag="PLV77_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNC15490.1"
                     /translation="MNFGRLLDLARGPSAQTLATSIASQALTLISGVVVARALGVEGR
                     GVLALLWLLPLTIVLLGGIGIPQATTFYVARELDNARAVVSISIRISLLLAVILSLGY
                     ATGLLLLSDGQEFSASDALLSSALIPMFLAQNLGVAALLGMKRYRAFNAGRIVPALLY
                     ALLVTTLFGLSIATLSSILIATVGAWAFGALVTWLLVFRKLPSTGEVAGAATSDVLSF
                     GLRGVIGSVSPIDDVRIDQLMVGLLMDSRALGLYVAAIAFCNLPRFIAQSIGAVSFPR
                     IASARSAAEAWQVAIRGKETAPML"
BASE COUNT          374 a          640 c          598 g          465 t
ORIGIN      
        1 cgacaatgtg ttcttgatgg cgaccaattt ccttccctgc ctagttcagg cttgaactct
       61 agcggactga attcttcacc aggcgctgca gcagtttgag tccgatcacg atcttgggag
      121 gctggcgata gctttcgttg aacacgcgac gatccaattc ctggttgatc tcaacaaccg
      181 ggggatgaac tatcgagtcc atcgaatcca acgccgcgtc gcccagaccc cggtagtcct
      241 tccatgcctg ggtgtgagtg gcacccgaac tgaatccgat gttcctgacc aaattcttcg
      301 tcgggtagat cgataaacct tgacatctcc agatcgagaa cgcccaagga tagtcccagg
      361 tgtcaatgcc accgtcgaag taatttttca acgttttccg ccagtatttc tcttcctccg
      421 cggtctgaaa ttgcccggcg aaggcttcga atacggattc aaattcattc gtggtcagcg
      481 aatagtcaga ccacgcgtcg cgccatgtcg cccagcccca aacagccgga tacttcgaga
      541 agaagtagct ctgttcggcg ggcaagtcct tgacgaggca tgatccggcg acgtgcatca
      601 cccgcttgtc gccccggtac ctgtccagca ggtcttcgca gaagaagaag aagctgcgat
      661 ccggcaggca gtcgtcttcg aggatgatgc cctcttcctc ctgctcgaag aaccagctga
      721 tcgccccgct gacggcgagc ctgcacccca ggttctcctt ccggaagagt gtcttgacct
      781 cgcaatccca gtcgatgccc tcggcgacga ggctacgaac gttctctact tcgatttcct
      841 cgccccgacg gtcgggcctc ggcccgtcag acgcaatgta catccgcggt ggcttcactt
      901 cgcgaatcgc ctcgagaacc gccttggtcg tcccgggacg attgaaaacg atcagcagga
      961 ccgcggactt caggtcggtc ggcatcagcg ggccagtata tccgcgcaca gcttccggct
     1021 tcctctctct ggctgacgga atcagggcgt tagtcatgtc cggattcagc tagcttcccg
     1081 gggagttact ggaccgcagt ggtggtccag tccaagactt gggcgatcat caagagaaag
     1141 aaacattcat gaagccagaa ggccggagag gcctggtgaa cttcggtcgg ctcctggacc
     1201 tggcccgcgg gccgagcgcc cagaccctgg ctacctcgat cgcaagccaa gccctgactt
     1261 tgatcagcgg ggtagtcgtc gcaagggccc ttggcgtcga aggccggggg gtccttgccc
     1321 ttctttggct gttgccgttg acgatcgtgc tgttgggagg gatcggcatt ccccaggcca
     1381 cgacgttcta cgtagcccgg gaactggaca acgccagggc cgtcgtgagc atttcaatcc
     1441 ggatctcgct gctgctggcg gtgatccttt ctctcgggta cgcaaccggg ttgcttctgc
     1501 tttcggacgg ccaggaattc tccgcctcgg atgccttgct cagttccgct ctgattccaa
     1561 tgttcctggc acagaacctc ggggttgcgg ctctgctcgg catgaagcgt taccgggcat
     1621 tcaacgcggg acggatcgtt ccggcgcttc tctacgcgct cctggtgacc actcttttcg
     1681 gtcttagcat cgccactctt tcgtcgatcc tgattgccac agtcggcgcc tgggcctttg
     1741 gcgctctggt cacctggctc ttggtctttc gcaagctgcc gtcaaccggc gaggttgccg
     1801 gagcagccac ctcggatgtc ttgagcttcg ggctccgggg agtaatcggc agtgtttcgc
     1861 cgatcgacga cgtgcggatc gatcagctga tggtcggcct gctgatggac agccgggcgc
     1921 tcggtctgta cgtggctgcg atcgccttct gcaaccttcc gagattcatc gcccagagca
     1981 tcggcgccgt ctcttttccg cggatcgcat ccgccagaag tgccgccgag gcctggcagg
     2041 tggcgatccg cggaaaagag acggcgccga tgctctg
//
LOCUS       DAOQMF010000338         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_36101_length_2076_cov_4.531915, whole genome shotgun sequence.
ACCESSION   DAOQMF010000338 DAOQMF010000000
VERSION     DAOQMF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36101_length_2076_cov_4.531915"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLV77_06290"
     CDS             <1..369
                     /locus_tag="PLV77_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC15491.1"
                     /translation="VSQVMDTEPVSIPAEIDCARALEEYFLRFGWSWFPVVDRHGSFL
                     GMAVRDRLEAVPEVERDATPVISLVEINPGFSVRSDAGLDRLLGNPQLRRFGALMVTD
                     DQGKLAGVVTVEQLGRALQA"
     gene            423..1670
                     /locus_tag="PLV77_06295"
     CDS             423..1670
                     /locus_tag="PLV77_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNC15492.1"
                     /translation="MAVATFLIVLALIASERVHRTKVALLGAAIVVLFVGQYNEELAI
                     ESVEFATLGLLAGMMILVYLTQQSGVYDYIAVKAGQLSRGRPFLLVVMLVTTVAVMSG
                     FLDNLTTILLIVPITFLLADTLDINPMPLILIEVMAANIGGAATLIGDPPNIMIGNSA
                     NLSFNDFLINNMPGVVVILATVTVGLYFAYRSQLQVAEENRKYVQELDAAASIRDHDE
                     LRRTLPVLIGTIILFFLHQYIHVEPATIALTGAAVGLMVTKTPIEEALEKIEWTTLFF
                     FIGLFVMVGALEVTGAIEKLAEGITSVTNGNRDAELVGIIWTSSLVGGIVDNIPLTAA
                     MIPVVEDIKAGSTDHAYWWALSLGACFGGNLTLISAACNVAASGMAEKAGRPIGFWTF
                     LKVGFPVTVASTVMATAYVLLRF"
     gene            1706..>2076
                     /locus_tag="PLV77_06300"
     CDS             1706..>2076
                     /locus_tag="PLV77_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC15493.1"
                     /translation="MADHIVRSVIREVKPLKTSDPVGYAARLVIESGLPALPVVDEHG
                     KYAGLFGEREFMTAVFPGYVGTLMSARMIRRSLDETIERRIGSQEEPVRGYLTTDPVL
                     VEDDYADTQLAETFLHHRVMVV"
BASE COUNT          375 a          658 c          651 g          392 t
ORIGIN      
        1 gtctcgcagg tgatggacac cgaaccggtt tcgatcccgg ccgagatcga ctgtgccagg
       61 gccctcgagg agtacttcct ccgcttcggt tggtcctggt tcccggtggt cgaccggcac
      121 ggctcgttcc tcggaatggc ggtcagggac cggcttgaag cggtacccga ggtcgaaagg
      181 gacgcgaccc cggtgatctc gctggtcgag atcaatccgg gcttttcggt gcgctccgac
      241 gccggcctcg accggctgct cgggaacccc cagctacgcc gtttcggggc cttgatggtg
      301 accgacgacc aggggaaact ggccggcgtg gtgactgtcg agcagctcgg ccgggctctt
      361 caggcctgat ttacgacctc gccgccttca accggcgcac tacatagact cggtatctgg
      421 cactggcagt cgcaacattt ctgatcgtcc tcgccctgat cgcgagcgaa cgggtccaca
      481 ggaccaaggt ggccctgctc ggtgccgcga tcgtggtgct cttcgttggt cagtacaacg
      541 aggaactggc gatcgaatcc gtcgagttcg ccacgcttgg tctgctcgcc ggaatgatga
      601 tcctggtcta cctgacccag cagagcgggg tttacgacta catcgcagtc aaggccggcc
      661 agctttcgcg cggccggcca ttcctgctgg tggtgatgct ggtcaccacc gtggcggtga
      721 tgtcgggttt cctcgacaac ctcacgacca tccttctgat cgtgcccatc accttcctgc
      781 tggccgacac cctggacatc aaccccatgc cactgatcct gatcgaggtg atggccgcga
      841 acatcggcgg tgctgcgacc ctgatcggtg acccgccgaa catcatgatc ggcaactcgg
      901 caaacctgag cttcaacgac ttcctgatca acaacatgcc cggcgtcgtg gtgatcctcg
      961 ctaccgtgac cgtgggcctc tacttcgctt accggagcca gctccaggtg gccgaggaga
     1021 accggaagta cgtccaggaa ctggacgcag ccgcatcgat cagggaccac gacgagctcc
     1081 ggcgcaccct tccggtcctg atcgggacga tcatcctttt cttcctccac cagtacatcc
     1141 acgtcgagcc ggcgacgatc gccctgaccg gagcggcagt cggactgatg gtcacgaaaa
     1201 caccaatcga ggaggccctg gagaagatcg agtggacgac gctcttcttc ttcatcgggc
     1261 tcttcgtgat ggttggtgcc ctggaggtga caggcgcgat cgagaagctg gccgagggaa
     1321 tcaccagcgt caccaacgga aaccgggacg ccgaactggt cgggatcatc tggacctcat
     1381 cactggtcgg cgggatcgtc gacaacatcc ccctgaccgc cgcgatgatc ccggtggtcg
     1441 aagacatcaa ggctggcagc accgatcacg catactggtg ggcgctctcg cttggcgcct
     1501 gcttcggagg caacctgacc ctgatctcgg ccgcctgcaa cgtcgccgca tcggggatgg
     1561 cggagaaggc agggaggccg atcgggttct ggacttttct caaggtcggc ttcccggtga
     1621 ccgtggcgtc gaccgtcatg gcaaccgcct acgtgttgct gaggttctga ccgatgaaac
     1681 cggctagttt ctgcgagatg ggcaaatggc agaccacata gtccgttcgg tcatccggga
     1741 ggtcaagccg ctcaagacca gcgacccggt cgggtacgcg gcgcggctgg tgatcgagtc
     1801 cggcctgccg gctcttccgg tggtcgatga gcacggcaag tacgccgggc tcttcggcga
     1861 gcgggagttc atgaccgcag tcttccccgg atacgtggga accctcatga gcgcccgcat
     1921 gatccggcgc tcactcgacg aaacgatcga gcgacggatc ggaagtcagg aggaaccggt
     1981 gcgcggatac ctgaccacgg acccggtcct ggtcgaggat gactacgccg acacgcagct
     2041 ggcggaaacc ttcctccatc accgggtgat ggtggt
//
LOCUS       DAOQMF010000339         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_36212_length_2073_cov_4.040139, whole genome shotgun sequence.
ACCESSION   DAOQMF010000339 DAOQMF010000000
VERSION     DAOQMF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36212_length_2073_cov_4.040139"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLV77_06305"
     CDS             complement(<1..236)
                     /locus_tag="PLV77_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15494.1"
                     /translation="MAAATLVLALGISACGSSSDDSGESSDTGTTTSTTQAPANAQSV
                     RIGLTEMAISASPDSVKAGDVAFDVTNDGNLEHEL"
     gene            complement(319..720)
                     /locus_tag="PLV77_06310"
     CDS             complement(319..720)
                     /locus_tag="PLV77_06310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNC15495.1"
                     /translation="MKLIVNVDGGARGNPGPAAIGIVVRDGEGNVLQDLGETIGEATN
                     NVAEYRALIKGIGLARELGATELQIHGDSELVVKQMLGQYKVKHPDMKPLHAEAKAAL
                     EGLDSWSISHVRREQNAEADALVNQALDSQA"
     gene            complement(775..1695)
                     /locus_tag="PLV77_06315"
     CDS             complement(775..1695)
                     /locus_tag="PLV77_06315"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNC15496.1"
                     /translation="MSALPSTSASCPAWGEALELFRRDLLAAGTAPRTLNAYEKDLGQ
                     LRAWADGSGLGVDKVTHKDLRRYAASLSAAGRAPATVARKLAAIRGFYGFLLRTGRVG
                     QNPADLVSAPRRKKKLPTVLTMEQMSSMLDAVPVETPLDIRDRAMFELTYSCGLRSEE
                     VLTLTLDSIDFDDEVIRVLGKGSKHRLVPVGEPARQAIEFYLARARPKLATDPGERTL
                     FLSRNGRPLSASDLSRRLAVRMRDAAAAGGVSPHALRHSFATHLLEGGADLRSIQELL
                     GHASISTTQVYTHLDAARLRDAYTGSHPRA"
     gene            complement(1667..>2073)
                     /locus_tag="PLV77_06320"
     CDS             complement(1667..>2073)
                     /locus_tag="PLV77_06320"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC15497.1"
                     /translation="EKYPTYLTLDRSNFTLTLWKNLEKVKSYTVAVGQVGLETPAGLY
                     SIQDKQVDPVWTVPNSAWAGDMAGQVVPGGVPENPLKARWMGIFDGAGIHGTDETYSL
                     GSAASHGCVRMSVPDVIDLYDRVDVGTPIYIG"
BASE COUNT          339 a          648 c          707 g          379 t
ORIGIN      
        1 agctcgtgct cgaggttccc gtcattggtc acgtcgaagg ccacgtcccc ggccttcacg
       61 gagtccggcg aagcgctgat cgccatctcg gtcagaccga tcctgacgga ctgggcgttg
      121 gccggagcct gggtagtcga ggttgtggtg ccggtgtcgc tgctctctcc ggaatcatcg
      181 ctcgaggatc cacaagcgga gattcccagg gcaagaacaa gcgttgccgc agccacagat
      241 acacgcaatg cgtttggctt caaagggagg cctttctgac taggttcacc aggacggaag
      301 acctggcgac gcttgagttt acgcctggga gtcgagggcc tggttgacca gcgcgtcggc
      361 ttcggcgttc tgttcacggc ggacgtgact gatcgaccac gagtcgaggc cttcgagcgc
      421 ggccttggct tcggcgtgga gcggcttcat gtccggatgc ttgaccttgt actgaccgag
      481 catctgcttg acgaccagtt ccgaatcacc gtggatctga agttcggtcg cgccgagttc
      541 ccgggccagg ccgatgccct tgatcagggc cctgtactcg gccacgttgt ttgtggcttc
      601 gccgatcgtc tcgccgagat cctggagcac gttgccctca ccgtcccgaa cgactattcc
      661 gatcgcagcc gggcccggat tgccccgggc gccgccgtcc acgttgacga tcagcttcat
      721 ggcagggaat ctattgcctg cgccgggcgg tcgactcccg gctgaagcgc cccttcaggc
      781 gcggggatgg ctgccggtgt acgcgtcccg caggcgggcc gcgtcgaggt gggtgtagac
      841 ctgtgtggtc gagatcgagg cgtggccgag cagttcctga atcgagcgca ggtccgcccc
      901 gccttcgagc aggtgggtgg cgaaggaatg cctgagggca tggggcgaaa ccccgccggc
      961 cgcggcggca tcccgcatgc ggaccgccag gcgacgggaa aggtcggagg ccgaaagggg
     1021 tcggccgttt cgcgaaagga acagggtcct ctcgcccggg tcggtggcca gcttcggccg
     1081 ggccctggcg aggtagaact cgatcgcctg ccgggccggc tcgccgaccg ggaccagccg
     1141 gtgcttggac cccttgccga gcacccggat cacttcgtcg tcgaaatcga tcgagtcgag
     1201 ggtgagggtc agtacctctt cgcttcgcag accgcaggag taggtgagtt cgaacatcgc
     1261 ccggtccctg atgtcaagcg gggtttcgac cggtaccgcg tccagcatcg aggacatctg
     1321 ttccatcgtc agaacggtcg gcagcttctt cttccggcgg ggtgcggaaa ccaggtcggc
     1381 cgggttctgg ccaacccttc cggtcctgag gaggaagccg taaaagccgc ggatcgcggc
     1441 cagtttgcga gccaccgtgg ccggggcgcg gcccgccgcc gaaagcgagg cggcgtagcg
     1501 gcgaagatcc ttgtgggtga ccttgtctac ccccaggcct gacccatcgg cccaggcccg
     1561 cagctggccg aggtcctttt cgtaggcgtt cagggttcgc ggtgccgtac ccgccgcgag
     1621 aagatcgcgc cggaagagct cgagagcctc gccccaggcg gggcagctag ccgatgtaga
     1681 tgggagtgcc gacatcgacc cggtcgtaaa ggtcgatcac gtccgggacc gacatccgca
     1741 cgcaaccatg cgaggcggcc gaaccgagcg agtaggtttc atccgtgccg tggatgcccg
     1801 caccatcgaa aatccccatc catcgagcct tcagggggtt ttcgggaacc ccgccgggaa
     1861 cgacctgacc ggccatgtct ccggcccagg ccgagttcgg caccgtccag acgggatcga
     1921 cctgtttgtc ctggattgag tagagcccgg cgggagtttc gaggccgacc tggccaaccg
     1981 cgaccgtgta gctcttgacc ttttcgaggt tcttccagag ggtcagggtg aagttgctcc
     2041 ggtccagggt cagataggtc ggatacttct cgg
//
LOCUS       DAOQMF010000340         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_36344_length_2069_cov_3.252731, whole genome shotgun sequence.
ACCESSION   DAOQMF010000340 DAOQMF010000000
VERSION     DAOQMF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36344_length_2069_cov_3.252731"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1224
                     /locus_tag="PLV77_06325"
     CDS             430..1224
                     /locus_tag="PLV77_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC15498.1"
                     /translation="MNAGRAPMRVAVIDTDSGFIRVLSNRMDAAGWQYRVLASGVPPE
                     ELVAMKINAVVFDPDVIGEGGWEYLERICGMLPDLGVIVCANGGTVSQRVRGLRIGAD
                     DWVNKPCHPEEVIARIEAVVRRRRRHSNEQDAGPLVVGEIEIRPDQFQAFIAGQSLDL
                     TRREFELIQLLAHNEGKVLDRESIYQRVWGYAMAHGDRSVDVFIRKLRTKLERRSPAY
                     TYIHTHFGVGYRFAAESSISAAEATAKVSSDRATDDAGAAAPGVPV"
     gene            1407..>2069
                     /gene="phoU"
                     /locus_tag="PLV77_06330"
     CDS             1407..>2069
                     /gene="phoU"
                     /locus_tag="PLV77_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNC15499.1"
                     /translation="MTEKIRIQYQEELDHLEQQALGAFDLVDRALGRTLEAIENQDVE
                     LAELVAADDDRIDGRYLEIHQGLITLLATQSPVATDLRLISALLHVIKNAERMGDQCV
                     NICKLIPIVGHEPPSDESMIRLILEMGVQASSQIIQARQAFADRNETAAQDLVRQDDL
                     IDRLNRDCFTLAIEIGDEPDRREWAMTMLLAARAIERIGDNAVDVGEQVAFVVTGLFR
                     EFE"
BASE COUNT          387 a          654 c          669 g          359 t
ORIGIN      
        1 aagggcaacg agactgttac ggacacgatt cacgccccga aacctaccgc aaaccgcagg
       61 acgcctgtgg ctacccgact gctccggcgc gggtgtgcct gttgctcctg gcccgggagt
      121 tgtgcctttg cggcacctcg ggatccgagg gcaggtgaga atggggcccg gcctggaaga
      181 tcgaggaggg agttctcggc atatggcgca cccaactccg acttcgatcc ggaccaccgg
      241 gccgagaacg gagttgggtg cgctatgtgg cgcaaattgg tacctcgctg ttttcgggac
      301 tcccggctcg cttcaggtcc agagccgaat cttttcgggg ttgtgaatga gttgtgaaaa
      361 tcggcttgat ttcgcggctt tgcgtccggc tacgccctac acttttcggt agatgatcgg
      421 aagctcaaaa tgaacgccgg ccgggctcca atgcgtgttg cggtgatcga caccgattcc
      481 ggcttcatcc gggtcctttc gaaccggatg gacgcggccg gctggcagta ccgggtcctc
      541 gcctcgggcg tgccgccgga ggaactggtc gcgatgaaga tcaacgcggt cgtgttcgat
      601 ccggacgtga tcggcgaagg cggctgggag tacctcgagc ggatctgcgg catgctgccc
      661 gacctcggcg tgatcgtctg cgccaacggg ggcaccgttt cacagcgggt ccgcgggctc
      721 aggatcgggg ccgacgactg ggtcaacaag ccctgccacc cggaagaggt gatcgccagg
      781 atcgaagcgg tggtgcgccg tcgccgtcgt cattccaacg aacaggacgc cggtccgctg
      841 gtcgtcgggg agatcgagat ccgccccgac cagttccagg ccttcatcgc cggccagagc
      901 ctcgacctga cccgccgcga gttcgagctg attcaactcc tcgcacacaa tgagggcaag
      961 gtcctcgacc gcgaatcgat ctaccagcgt gtctggggct acgcgatggc ccacggcgac
     1021 cgctcggttg acgtcttcat ccgcaagctc cgcaccaagc tggagaggcg gtccccggcc
     1081 tacacctaca tccacaccca cttcggcgtc ggctacagat ttgcggccga atcgtcgatt
     1141 tcggccgccg aggcgaccgc caaggtcagc tccgaccggg ccaccgatga tgcgggcgct
     1201 gcggcgcccg gcgtgccggt ctagcgctgc accttctctc actcacgaaa acgggcaccc
     1261 gatcaagggt gaacccgctt cacaggagtt cacaactctt ttacagcagg tctacccgca
     1321 ggtaacaaac tggggcggaa gcggtggaac gcttcgaacc accaggccgc ccccgaaagg
     1381 cgggaaccaa gtcccggagg gtgtgagtga ccgagaagat ccgaattcag tatcaggaag
     1441 aacttgacca cctcgagcag caggctctcg gggccttcga tctcgtggac cgggcgctag
     1501 gccgcaccct cgaagcgatc gaaaaccagg acgtcgagct ggccgaactg gttgctgccg
     1561 acgacgaccg gatcgacggg cgctacctcg agattcacca gggtctgatc accctgctgg
     1621 ccacccagtc cccggtggcc accgaccttc ggctgatttc cgctctgctc cacgtgatca
     1681 agaacgcgga gcggatgggg gatcagtgcg tgaacatctg caagctgatc ccgatcgtcg
     1741 gccacgagcc gccttcggac gagagcatga tccggttgat cctcgagatg ggggtccagg
     1801 ccagcagcca gatcattcag gccaggcagg ccttcgccga tcgcaacgag acggccgccc
     1861 aggacctggt ccggcaggac gacctgatcg accggctgaa ccgcgactgc ttcaccctgg
     1921 cgatcgaaat cggcgacgag cccgaccgcc gtgaatgggc gatgacgatg ttgctggccg
     1981 cgcgtgcgat cgagcggatc ggcgacaacg cggttgatgt cggggaacag gttgcctttg
     2041 tggtgaccgg cctgttccgg gaattcgag
//
LOCUS       DAOQMF010000341         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_36432_length_2066_cov_2.788165, whole genome shotgun sequence.
ACCESSION   DAOQMF010000341 DAOQMF010000000
VERSION     DAOQMF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36432_length_2066_cov_2.788165"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="PLV77_06335"
     CDS             <1..1769
                     /locus_tag="PLV77_06335"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15500.1"
                     /translation="DCGTTASHNTCYSIFGNGQPVTLTANPDSDSLFNSWGGDCSGTQ
                     PTCTVTLDQTRSVTSSFSHGPRKLTVTRPGNGAGGVTSTPAGIDCGTTCEANFPYGTA
                     VTLTPGASTGSSFTGWTGACSGSGACSVTMDQARTVGATFTLQKRTLTLAKFENGSGT
                     VVSSPAGINFPTGATSCLVDGKCVNAQFDYGTEVTLTPTAATGSTFTGWGGACQSFAI
                     DQNCVVTMDQLNTAEAGFVLQKRTLEVAKTGSGTGGVTSATPGIDCGSDCSNDYDYGT
                     EVTLNPTPGPNSLFTGWSGACTGTAACVVTLDQARQVTASFALEKRSLGVTRSGNGSG
                     TVMSQPAGVACGGQCEGIEFDYGTEVTLSANPATSSDFSGWTGDCSGSGSTCIVTLDQ
                     ARSVEATFTLQKRTLTVETSGTGSGTVGTDPAGPDFDFGTPVTLEAVPANGSTFTGWG
                     GDCSGIVPTCTVDMSDARSVEARFTADPLPDPAPVIGQLRVAPRKVPLKGKKKSAIRR
                     QGPAIRIRLSEASTVRFSLKPKPGKARNFRRKLKAGISKVRIPAGVRKLLRPGRFQLT
                     AVATDNAGQSSNPRRTGFRVIR"
     gene            complement(1766..2044)
                     /locus_tag="PLV77_06340"
     CDS             complement(1766..2044)
                     /locus_tag="PLV77_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HNC15501.1"
                     /translation="MSGAAPPPGQAPAGSTIKVKRETSGRKGKTATTISEIPLGEAET
                     RALAKKLKQLCGVGGTARGSSIELQGDHRDKVIAYLEKEGHRAVKAGG"
BASE COUNT          353 a          728 c          648 g          337 t
ORIGIN      
        1 ttgactgcgg caccaccgct tcccacaaca cctgctactc gatcttcgga aacggccagc
       61 cggtgaccct caccgccaac cccgattccg actcgctctt caacagttgg ggcggggact
      121 gctcgggcac ccagccgacc tgcaccgtca ctctggatca gacccggagc gtcacctcat
      181 ccttctctca cggaccgcgg aagctcacgg tcaccaggcc gggcaacggt gcgggaggcg
      241 tcacctcaac cccggccggc attgattgcg gaaccacctg cgaggcgaac ttcccctacg
      301 gcaccgcggt cacgctgacg ccgggtgcct cgaccggttc cagcttcacc ggctggaccg
      361 gcgcctgttc cggctcgggc gcgtgctcgg tgacgatgga ccaggcccgg accgtcggag
      421 cgacattcac ccttcagaag cgcaccctga ccctggcgaa gttcgagaac ggatcgggaa
      481 ccgtcgtctc gagcccggcg ggcatcaact tcccgaccgg agcgacatcc tgcctcgtcg
      541 acgggaaatg cgtgaacgcc cagttcgact acggcaccga ggtaacgctg accccgaccg
      601 cggcgaccgg gtcgaccttc accggctggg gcggagcctg ccagtcattt gcgatcgacc
      661 agaactgtgt cgtgaccatg gaccagctca acaccgccga agccggcttc gtgctgcaga
      721 aacggaccct ggaagtcgcg aagaccgggt cggggacggg cggagtgact tcggccactc
      781 ccggcatcga ttgcggaagc gactgttcga acgactacga ctacggcacc gaggtcacct
      841 tgaaccccac tcccgggccg aactcactgt tcaccggctg gagcggggcc tgcaccggaa
      901 ccgcggcttg cgtggtcacc ctggatcagg cccgtcaggt cacggcctcc ttcgcgctcg
      961 agaagcgcag cctgggcgtg acccggtccg gcaacggttc gggcacggtc atgtcgcagc
     1021 cggcgggagt cgcctgtggt ggccaatgcg aaggcatcga gttcgattac ggcaccgaag
     1081 tgaccctctc ggcgaacccg gccacgagtt ccgatttcag cggatggacc ggggactgct
     1141 cgggatcggg ttccacctgc atcgtcaccc tcgaccaggc ccgttccgtc gaggccacgt
     1201 tcacccttca gaagcggacg ctgacggttg agacctcagg caccgggtcg ggcacagtcg
     1261 gtacagaccc ggccggcccc gatttcgact tcggaactcc ggtcaccctg gaagcggttc
     1321 ccgcgaacgg ctcgactttc accgggtggg gcggggactg ttccggaatc gtgccgacct
     1381 gcacggtcga tatgtcagac gcccggtcag tcgaggccag gttcaccgcc gacccgttgc
     1441 cggaccccgc accggtgatc ggtcagctca gggtcgcgcc gcgaaaggtc ccgctcaagg
     1501 gcaaaaagaa gtcggccatc cggcgccagg gaccggcgat caggatcagg ctaagcgaag
     1561 catcgaccgt tcgtttcagc ctcaaaccga aaccgggcaa ggccaggaac ttcaggcgca
     1621 aactcaaggc cggcatcagc aaggtccgga tcccggccgg ggtccgcaag ctcctcaggc
     1681 cgggaaggtt ccagctcacc gccgtagcga ccgacaacgc cggtcagagc tcgaacccac
     1741 gccgaaccgg attcagggtc atccgctagc ccccggcctt tacggcccgg tggccttcct
     1801 tttcgaggta ggcgatcacc ttgtcgcggt ggtcgccctg gagttcgatg ctggaacccc
     1861 tcgcggtccc gccgaccccg caaagctgct tcagcttctt cgccagggct ctggtctcgg
     1921 cttcgcccag ggggatctcg gagatcgtgg tggcggtctt ccccttccgg cccgaggttt
     1981 cccgcttcac cttgatcgtc gagccggccg gcgcctggcc gggcgggggt gccgcgccgg
     2041 atatgcctcc gcgccttttc gccttt
//
LOCUS       DAOQMF010000342         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_36656_length_2059_cov_3.656687, whole genome shotgun sequence.
ACCESSION   DAOQMF010000342 DAOQMF010000000
VERSION     DAOQMF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36656_length_2059_cov_3.656687"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /gene="ilvB"
                     /locus_tag="PLV77_06345"
     CDS             complement(<1..1168)
                     /gene="ilvB"
                     /locus_tag="PLV77_06345"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNC15502.1"
                     /translation="MRAADALFRSLEAEGVDHIFGIPGGANLPTYDALVDADLRHVQA
                     RHEQAAGHAAEGYAHASGKVGVAFGTSGPGATNLVTAIADAMMDSVPTVFVTGQVRTD
                     LIGTDGFQEADIVGITLPIVKHSLLVTEPEQIPEFVHEAFHIASSGRPGPVLIDIPQD
                     LARAEIDYTPRTEPVDLAGYKPTVEGNLKQIRLAAKAMANARRPVIYSGGGVKNGNAS
                     MELRELCATDNFPVTSTLMGLGCFPADSDQWLGMLGMHGTRTANYAMDEADLIIAIGS
                     RFDDRITGKLSEFAPSAKFIHIDIDPAEISKNVPAHIPIVGDVKQILPKLTREYQALQ
                     TDSARLEGWWSRLKAWQGEYPLTYEPGADGEIKPQFMIEMMHKVTGGDAIVTSDV"
     gene            complement(1205..>2059)
                     /locus_tag="PLV77_06350"
     CDS             complement(1205..>2059)
                     /locus_tag="PLV77_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC15503.1"
                     /translation="ETQQTNVIGTLNLLFSMREHVPDAHLIKLGTMGEYGTPNIDIEE
                     GFIEIEHNGRKDTLPFPKLPASLYHCSKVHDSTNIHFATRVWGLRATDLNQGVVYGIE
                     TDETALDPALATRFDYDETFGTVLNRFCVQAVIGHPLTVYGAGGQTRGYLNIRDTLRC
                     VELATLNPAERGEFRVFNQFTEHFTVAELAQLVQRCAAEVGHEVSIETFPNPRVELES
                     HYYNPKNQKLLDLGLEPHYLGEELVRSVLSTIEQYKDRVIPRAITPKTKWNPGELEGG
                     ALSAAQKT"
BASE COUNT          320 a          620 c          722 g          397 t
ORIGIN      
        1 cgacgtcgga agtcacgatc gcgtcgccgc cggtgacctt gtgcatcatc tcgatcatga
       61 actggggctt gatctcgccg tcggcccccg gttcgtaggt aagcgggtac tcgccctgcc
      121 aggccttgag ccggctccac cagccttcga gccgggcgct gtcggtctgg agcgcctgat
      181 actcgcgggt cagcttcggc aggatctgct tgacgtcacc gacgatcggg atgtgggccg
      241 ggacgttctt cgagatctcg gccgggtcga tgtcgatgtg gatgaacttg gccgaagggg
      301 cgaactcgga aagcttgccg gtgatccggt catcgaagcg cgacccgatc gcgatgatca
      361 ggtcggcctc gtccatcgcg tagttcgcgg tccgggttcc gtgcatgccg agcatgccga
      421 gccactggtc gctgtcggcc gggaagcagc ccagacccat cagggtcgag gtgaccggga
      481 agttgtctgt ggcgcagagc tcacgcagct ccatcgaggc gttgccgttc ttgactcctc
      541 cgcccgaata gatcaccggt cgccgggcgt tggccatcgc cttggcggcg aggcggatct
      601 gcttcaggtt gccttcgacg gtcggcttgt agccagcgag gtcaaccggc tcggtccgcg
      661 gggtgtagtc aatctcggcc cgggccaggt cctgcgggat gtcgatcagc accggaccgg
      721 gacggccgct cgaggcgatg tggaatgcct cgtgaacgaa ctccggaatc tgttccggct
      781 cggtgaccag cagcgaatgc ttgacgatcg gcagcgtgat gccgacgatg tcggcctcct
      841 ggaagccgtc ggtgccgatc aggtcggtgc ggacctgtcc ggtcacgaag acggtcggga
      901 ccgaatccat catcgcgtcg gcgatcgcgg tgaccaggtt ggtcgcgccg gggccggagg
      961 tgccaaaggc gaccccgacc ttgccggagg cgtgcgcgta accctcggcg gcgtggccgg
     1021 cggcctgctc gtgacgggcc tgtacgtgcc gcagatcggc gtcgaccagc gcgtcatagg
     1081 tgggcaggtt tgcgccaccg ggaatgccga agatgtggtc aacaccttcc gcttcgaggg
     1141 atctgaacaa tgcgtctgcg gctctcatcg gaccaacgat cctaacgaat cactgcctgg
     1201 gggctcaggt cttctgagcg gctgaaagcg cgccgccttc gagctcgccc ggattccact
     1261 tggtcttcgg ggtgatcgcc cggggaatga cccggtcctt gtactgctcg atggtggaaa
     1321 gcaccgatcg gaccagttcc tcgccgaggt aatgcggctc gagaccgagg tccagcagct
     1381 tctggttctt cgggttgtag tagtggcttt ccagctcgac ccgcgggttg gggaaggtct
     1441 cgatcgagac ttcgtggccg acttcggccg cgcagcgctg gaccagctgg gccagctcgg
     1501 ccaccgtgaa gtgctcggtg aactgattga agacccggaa ctcgccccgc tcggccgggt
     1561 tcagggtggc cagctcgacg cagcgcaggg tgtcgcggat gttcaggtaa ccgcgggtct
     1621 ggccgccggc cccgtacacg gtcagcgggt gaccgatcac ggcctggacg cagaaccggt
     1681 tcagcacggt gccaaaggtc tcgtcgtagt cgaagcgggt ggccagcgcc gggtcgagcg
     1741 cggtttcgtc ggtttcgatc ccgtagacga cgccctggtt gaggtcggtg gcacggagcc
     1801 cccagacccg ggtcgcgaag tggatgttgg tcgagtcgtg aaccttcgaa cagtggtaga
     1861 gcgaggcagg cagcttcggg aacggcagcg tgtccttgcg gccgttgtgc tcgatctcga
     1921 tgaagccttc ctcgatgtcg atgttgggcg tgccgtactc acccatcgtg ccgagcttga
     1981 tcaggtgggc atccggcacg tgctcgcgca tggagaagag caggttcagg gtgccgatca
     2041 cgttggtctg ctgcgtttc
//
LOCUS       DAOQMF010000343         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37048_length_2048_cov_3.277973, whole genome shotgun sequence.
ACCESSION   DAOQMF010000343 DAOQMF010000000
VERSION     DAOQMF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37048_length_2048_cov_3.277973"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLV77_06355"
     CDS             complement(<1..183)
                     /locus_tag="PLV77_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNC15504.1"
                     /translation="MSQIKMVISDFGGVLTTPLVEAFASVQDRVGIPPKTFGEALRAL
                     NEREGTNPLFELECGRI"
     gene            complement(180..923)
                     /locus_tag="PLV77_06360"
     CDS             complement(180..923)
                     /locus_tag="PLV77_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15505.1"
                     /translation="MKTRSIEILDPESGLFVSLTSGGNGAIMLGEDVVAAAPIPANGL
                     DPLVDGSPLELETEHGELAVSIESTGEPISFDGELTGRREASLVKAQGRLVHRDQEIG
                     LDCRGVLHRREEDAPETAGLSRDATIILADGGLICIAAAAPEGDFDHGDEETVAAITH
                     PGGYIEFDEVLLSTEYDTAGRQRRATLELWPATDEVAALHGAGSVIAGCTAKVGGSTI
                     NTALFRWSLDGHLGLGRYEITRPAAVAVA"
     gene            complement(895..1440)
                     /locus_tag="PLV77_06365"
     CDS             complement(895..1440)
                     /locus_tag="PLV77_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNC15506.1"
                     /translation="MFVLLLALLIIWPIAELFVMVQVADWIGFFWMLFLLFASTVAGI
                     MILKHRGRAHWRRFRGAIDERRPPAKEAFDGVMITAGALLLIIPGFITSAIGLFLLFP
                     PTRWLVRLVALTLFASKFKVAATTATWSTRGYNYYRGSRQGSYDIDGEAVDVTDRPDG
                     EPLPQLRSGSERGEDQIDRNP"
     gene            1585..1902
                     /locus_tag="PLV77_06370"
     CDS             1585..1902
                     /locus_tag="PLV77_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNC15507.1"
                     /translation="MAEDHATMPTEEEVFEALEEVIDPELGLDFVSLGLVYDVALEAP
                     DVYVTFTLTTPACPIGPQVSEQMKEFVGGLEGVRDVHPKMIFDPPWSPEMMTDDAKFA
                     LGF"
BASE COUNT          352 a          679 c          673 g          344 t
ORIGIN      
        1 gatccggcca cactcgagct cgaagagcgg attggtgccc tccctctcgt tcagcgcccg
       61 cagcgcctcg ccgaaggtct tgggcgggat cccgacccga tcctgcaccg aagcgaatgc
      121 ctcgaccagc ggggtcgtca gcaccccgcc gaaatccgag atgaccatct tgatctggct
      181 catgcgaccg ctaccgccgc gggacgggtg atctcgtaac ggccgaggcc gaggtggcca
      241 tcgagcgacc agcggaagag ggcggtgttg atcgtcgagc ccccgacctt cgcggtgcag
      301 ccggcgatca ccgatccggc accgtgaagc gcggccacct cgtcggtggc cggccagagc
      361 tcgagggtgg cccgacgctg gcgcccggcc gtgtcgtact cggtcgaaag caggacttca
      421 tcgaattcga tgtaaccacc ggggtgggtg atcgccgcca ccgtctcctc gtcaccgtgg
      481 tcgaagtcac cttccggggc ggccgcggcg atacagatca gcccgccgtc ggcaaggatg
      541 atcgtcgcgt cgcggctcag gccggcggtt tccggcgcgt cctcctcacg ccggtggagc
      601 accccgcggc aatcaaggcc gatctcctgg tcgcggtgga cgaggcgtcc ctgggccttg
      661 accaggctgg cctcacgccg gccggtcaac tcgccgtcga agctgatcgg ctccccggtc
      721 gattcgatcg aaacggcgag ttcaccatgt tcggtttcca gttcgagtgg tgacccgtcg
      781 accagcgggt cgagcccgtt ggccgggatc ggagccgccg cgacgacatc ttcaccgagc
      841 atgatcgcgc cgttgccgcc gctggtcagg ctgacgaaga gtccgctctc ggggtcaagg
      901 atttcgatcg atctggtctt caccgcgctc tgacccggag cgtagctggg ggagaggctc
      961 accgtcgggc cggtcggtca cgtcaaccgc ttcgccgtcg atgtcgtagg aaccctggcg
     1021 ggagccccgg tagtagttgt agccacgagt gctccaggtc gcggtggtgg ccgcgacctt
     1081 gaacttggag gcgaagaggg tcagcgcgac caggcgaacg agccacctgg tcggcgggaa
     1141 gagcaggaaa agaccgatcg ccgaggtgat gaagccgggg atgatcaaaa gcagcgcgcc
     1201 ggcggtgatc atcaccccgt cgaaggcttc cttcgctggc ggtcgtcgct catcgatcgc
     1261 cccgcggaac cggcgccagt gcgctctgcc gcggtgtttg aggatcatga tgcccgccac
     1321 ggtcgaggcg aagagcagga acagcatcca gaagaagccg atccagtcgg ccacctgcac
     1381 catcacgaaa agttccgcga tcggccagat gatcagcaat gccaggagca gtacgaacat
     1441 gggcagatac aggttacgca cccgggcaat ccgggttaca gcctgcggtg ggtcgggttt
     1501 ccgtcacccc ggccggaaac ccgacccacc ggccgaagca gcacgggctg gcggccggaa
     1561 tcccgaccca gctaggatgc attcatggct gaagaccacg caaccatgcc gaccgaagaa
     1621 gaggttttcg aagcgctcga ggaagtgatc gaccccgaac tcgggctcga cttcgtttca
     1681 ctggggctcg tctatgacgt tgcgctggaa gcgccggacg tctacgtgac attcaccctg
     1741 accacgcccg cctgcccgat cggaccccag gtctccgagc agatgaagga attcgtcggc
     1801 gggctcgagg gcgtacgcga cgttcatccg aagatgatct tcgacccgcc ctggtcgccg
     1861 gaaatgatga ccgacgacgc caagttcgcg ctcggcttct agcccttcgg gctgctcttc
     1921 ttcgccgccc ggcccgggcc gccgccgttg ctggcagtgg ccttcccggg gctgcccttc
     1981 cgggctgaac cctttctggc cgaagccttc ccgggtttgt cgggggcgat cgagttctcg
     2041 acctcttc
//
LOCUS       DAOQMF010000344         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37201_length_2044_cov_2.399196, whole genome shotgun sequence.
ACCESSION   DAOQMF010000344 DAOQMF010000000
VERSION     DAOQMF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37201_length_2044_cov_2.399196"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..>2044)
                     /locus_tag="PLV77_06375"
     CDS             complement(47..>2044)
                     /locus_tag="PLV77_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15508.1"
                     /translation="MAAIFAKGGEGLRALAAGPGRGRILLLAATAAVAVALLAPVTFA
                     HLNGLPLLVCLGLAGLGTWLWGRVRLPVLRGWKGVACDLLLIALVLLAVPDIPIYRVG
                     DLASQPVDSYLAEVMVFHGNLYLGAANAILHGSHLLVDEVSQYGVGSIYLLAALFELI
                     PIDYGTLSLIEAVLSAAVFAAGYLILRMAGNGRLLGLGAMAVTVIVLAWGLTFPIGGI
                     VQNGAIRFGLLPVSLVFFRIASIRLAGGGRPGLARVSGWLAWAVVGLSAIWALEALLY
                     TTATLTGLVLAEAMTRPPGSRRRWILGRVVRALIAWAGFHLAFAIYILIAAGQLPDWG
                     MYISYLRDFLVGKVGDLTYDFEPWSPGLLVGFGYLASATATALAIWRRRDWFDANQVA
                     FIALVGLSLYGLIQFSYFDNRSLATILPFLCFPLLLSATIWLDLIQRDPGAGPALRRG
                     AVAVALSLAAVTVSTVWPDARERSTDTALAYALPGGRSLADGLERLGDMPPLTGGADE
                     GERLLRKYMPGESESAVATRYDLDLNILIRADRANRLGIADANEASWVPGPHVPVMEE
                     AVDSLASGDRMLVDQGALRQFSLLQQDPEALPDEFTTDDGQTVIQLMALARIGRRFDL
                     KTVARGSDGLEVVELVPRGSGDELAGGKDPRPVQDQAESGP"
BASE COUNT          380 a          716 c          691 g          257 t
ORIGIN      
        1 gtcctcggtc ggtttcagga ccggggtggt ctacgggctg aagaccctat gggccggatt
       61 ccgcctgatc ctgcaccggg cgcggatcct tccctcccgc aagttcatcg cctgaacccc
      121 ggggcaccag ctcgaccacc tcgagtccgt cgctgcccct ggcaaccgtc ttcaaatcga
      181 agcgccggcc gatccgcgcc aatgccatca gctggatcac ggtctggccg tcatcggtgg
      241 tgaactcgtc cggcaacgct tccgggtcct gctgaagcag gctgaactgg cggagtgcgc
      301 cctggtcgac cagcatccgg tctccggacg caagcgagtc gaccgcttcc tccatgaccg
      361 gaacatgcgg tcccggcacc cagctcgcct cgttcgcgtc ggcgatgccc agccggtttg
      421 cccgatcggc ccggatcagg atgttcaggt ccaggtcgta gcgggtcgcc accgcactct
      481 cgctctcgcc gggcatgtac ttccggagca gccgttctcc ttcatcggcg ccgccggtaa
      541 ggggcggcat gtcaccgagc cgctccaggc cgtcggcgag gctgcggcct cccggcaggg
      601 cataggcgag agcggtatcg gtggaccgct ccctggcgtc cggccagacg gttgacacgg
      661 tgaccgccgc gaggctcaag gcaaccgcga ccgcaccccg ccgaagcgcc ggcccggctc
      721 cgggatcgcg ctggatcaga tccagccaga tcgtcgccga gagaagcagc ggaaagcaga
      781 ggaaggggag gatcgtcgcc agcgaacggt tgtcgaaata gctgaactgg atcaggccgt
      841 agaggctgag gccgaccagc gcgatgaagg cgacctggtt ggcgtcaaac cagtcgcggc
      901 ggcgccagat cgcgagcgcc gtcgcggtcg ccgaggcaag gtagccgaag ccgaccagca
      961 gccccggtga ccagggttcg aagtcatagg tcaaatcgcc gaccttgccg accaggaagt
     1021 cccgcagata cgagatgtac atgccccagt ccggcaactg gccggccgcg atcaggatgt
     1081 agatcgcgaa ggcgaggtga aagccggccc aggcgatcag ggcgcggacg actctcccca
     1141 gaatccagcg gcgacggctc cccggcggcc gggtcatcgc ctcggccaga accagcccgg
     1201 tcagggtggc ggtcgtgtaa agcagcgcct ccagggccca gatcgccgaa agcccgacca
     1261 ccgcccaggc gagccagccc gagacccggg ccagaccggg ccgccccccg ccggccagcc
     1321 ggatcgaggc gatccggaag aagaccagcg acacgggaag gaggccgaac cggatcgccc
     1381 cgttctggac gatcccaccg atcgggaagg tgagacccca ggcgagcacg atcacggtca
     1441 ccgccatcgc tccgagcccg agcaggcgac cgttcccggc catccgcagg atcaggtagc
     1501 cggccgcgaa gaccgccgcc gaaaggaccg cttcgatcag gctcagggtt ccgtaatcga
     1561 tcgggatcaa ctcgaagagg gcggccagca ggtagatcga accgaccccg tactgggaaa
     1621 cctcgtcgac cagcaggtgg ctgccgtgga ggatcgcgtt ggccgccccg aggtaaaggt
     1681 tgccgtggaa gaccatcacc tcggccagat atgaatcgac cggctgggag gcgaggtccc
     1741 cgacccggta gatcgggatg tccggcaccg ccaggagcac cagggcgatc aacagcaggt
     1801 cacaggcgac tcccttccag ccccggagga ccggcaggcg gacccgcccc cacagccagg
     1861 tgccgagccc ggccaggccc aggcagacca gaagcggcag cccgttcagg tgggcgaagg
     1921 tgaccggcgc cagcaacgcg acggccaccg cggcggtcgc ggcgaggagc aggatccggc
     1981 cgcggcccgg gccggcggcc agagcccgca ggccctcgcc tcccttggcg aatatcgccg
     2041 ccat
//
LOCUS       DAOQMF010000345         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37202_length_2044_cov_2.238311, whole genome shotgun sequence.
ACCESSION   DAOQMF010000345 DAOQMF010000000
VERSION     DAOQMF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37202_length_2044_cov_2.238311"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLV77_06380"
     CDS             complement(<1..196)
                     /locus_tag="PLV77_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15509.1"
                     /translation="MVAAFFRFLWLPWQLRGFSVFRFDYAGVIRSFEVTRGDRFESWA
                     RVPRLRSLPLAALAPLAACLV"
     gene            1246..>2044
                     /locus_tag="PLV77_06385"
     CDS             1246..>2044
                     /locus_tag="PLV77_06385"
                     /inference="COORDINATES: protein motif:HMM:NF025843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC15510.1"
                     /translation="MPDQFEITIERSIFRAPDSPFAILQGTSKDGHLRTVCGALESFA
                     HGDRLTVTATRSDHPRFGEQWKVTEAALMEPDDRPSRRAFLQSIDGIGPGRADELLDL
                     YGDDVFDRIDEAPIKVFSTLPGVGKKTSEKAARSWRERRQIKEVLVLLQEARIEASQG
                     VAARASRTFGGRTLEILEENPFALMELHGVGFRDADRLATHLGFPPDSPRRVKAGCQH
                     ILREAKATEGHLFLPVSDLTGRACDLLELPEERLDLTRTLTGAKGIVV"
BASE COUNT          564 a          616 c          554 g          310 t
ORIGIN      
        1 ccaccaaaca ggccgccaac ggagcaagcg ccgccaacgg caacgaacga agacgcggca
       61 cacgcgccca agactcaaag cgatcgccac gggtcacctc gaaagagcgg atcacccccg
      121 cgtaatcgaa acggaacaca ctgaaacccc tcaactgcca aggcagccaa aggaacctga
      181 aaaaagcggc gaccatagat cgcctccttt catgaattgg atttgggaac gaacaaaccg
      241 gccctcccgc gaggaccaca aaccatcgaa accggcgcct ccccccggac cccccaccaa
      301 ggaaaacccg gcgaaagcac agcccgaaaa caggaccgat cgaaacgacc aggcaggcca
      361 gccgacaaag gctcggcaag aaaaaccagg gacagaatcg acaggaaaca agcaggcgaa
      421 aacgacccgg ccaaagacac cagccaggtc gaaaacacca gagggaaaac aaggtcaaag
      481 acgaggcaag acgcagccga aaaaccgatc ggcaacaaga ccaaaaccag gtccgaaacc
      541 agagcagaaa cagagcctgt aaacgagcca gacaagaagg ccaaaaccaa ccagtcgaga
      601 cacgatcgtc aacaaaccca tactcctaaa acgtcgcgat agcgacgccg tcaaccagac
      661 ccgaaacgag actaggaaga agaaccaaaa cagcccggga aacgatcggg acaaagtgac
      721 aggacaggca aggaaacagg ccagaaagaa agctcaggga gaagaaccaa aagcatctgt
      781 cgacacgaac cagaaaccag aacaccagca agcaacgaag aacgagaagc aggtaactgg
      841 aaagcctgtc ggctacagaa acgtatgtct gggaactgcg gtcacaccgt ggcgtgaata
      901 agcgggttgc tcaatagagc gaaaaaagaa gagggccgca gcctaacccc tacgggggtc
      961 ctgtcaagcc gccagggttg ccttccctgg acctcgaatt ctcacctact ctctctgtct
     1021 cttaaccttc ctccggtctt cgtttcacac cggcaccgct caaccatgcg atttccggtc
     1081 tttctttgtg aaacgctcgc gtaggcttag gggccgctgt gtttcgtgaa atcgcctaat
     1141 tgacccgctg tgtttcaaga gtttcattcg ggtctttttc ggcgcgaccc gccacctaca
     1201 gtgtccgcga aaaacggcct gtctcgcagc taatgtcggt gctagatgcc agaccagttt
     1261 gagataacga tcgaaagatc catcttccgg gcacccgatt cgcccttcgc gatccttcag
     1321 ggaaccagca aggatgggca cctccgaacc gtctgcgggg cactcgagag ctttgcccat
     1381 ggcgaccgcc tgacagtcac cgctacacgg tcagatcacc cgcgattcgg ggagcagtgg
     1441 aaggtcaccg aggcggcatt gatggagcct gacgaccgtc cctcgagaag ggcttttctg
     1501 cagtcgatcg acggcatcgg accgggtcgc gctgatgagc tcttggacct ctacggagac
     1561 gatgttttcg accggattga cgaggctccg atcaaggtct tttcgaccct gcctggggtt
     1621 gggaaaaaga cctctgagaa ggcggcaaga agctggcgtg aacgacgtca aatcaaggag
     1681 gttcttgtgc tgctccagga ggcccgaata gaggcaagcc aaggggtcgc ggcccgggcc
     1741 tcaaggacat ttggaggccg caccctagag atcctcgaag agaatccttt cgctctcatg
     1801 gaacttcacg gggtcggttt cagggacgct gacaggctcg ccactcatct cggctttccc
     1861 cctgattcac ctcgccgcgt caaggccggc tgccagcata ttctccgtga ggcgaaagcc
     1921 accgaagggc atctatttct tcccgtcagc gacctgaccg gacgggcatg tgatctcctg
     1981 gagcttccgg aagagcggct tgatctgacc cgcacgctga ccggcgctaa gggaatcgtg
     2041 gtct
//
LOCUS       DAOQMF010000346         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37454_length_2036_cov_3.823322, whole genome shotgun sequence.
ACCESSION   DAOQMF010000346 DAOQMF010000000
VERSION     DAOQMF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37454_length_2036_cov_3.823322"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLV77_06390"
     CDS             <1..372
                     /locus_tag="PLV77_06390"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF020720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNC15511.1"
                     /translation="QEVSFEVEVSSGTYVRTLVETLGDAYCSKLRRTAVGPLDISLAG
                     RVLAPLEALSFLPQIELDAEQARAIGFGQKLDTGRFDLPSGIQAGSSKSFALVHGDNL
                     IAVARIEDSVLRPEVVLVPAS"
     gene            369..1253
                     /locus_tag="PLV77_06395"
     CDS             369..1253
                     /locus_tag="PLV77_06395"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNC15512.1"
                     /translation="MIKVTPLDEAEYRPRKIAIGTFDGVHVGHRAVIKGADTVLTFEP
                     HPLEILHPAAAPKLLMPLDVRRDVLDGLGVEEMIVIQFDDSFTKITAEQFIEEILIAR
                     LGAEEVSVGDNFRFGQKAKGTPEMLAARPEFRTRVEPLVEVDGEAVSSTRIRALVAAG
                     DMELARRCLGVPFMVEGEVVTGDQRGRELGFPTANIVPDDRFAHPGHGVYAAFADGLP
                     AAVNIGVRPTFDTGRGVLIETYIIDEEVDLYGRILRVAFVERLRGEKRFDSVDDLIAQ
                     MKRDVEKTKAVCASFHRP"
     gene            1295..1564
                     /gene="rpsO"
                     /locus_tag="PLV77_06400"
     CDS             1295..1564
                     /gene="rpsO"
                     /locus_tag="PLV77_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNC15513.1"
                     /translation="MTLTKENKAELISKYGKSEGDTGSTEVQVALLTERINDLTGHLR
                     EHGKDHHSRRGLLMMVGKRRRLLRYLERTDVERYRALVADLGLRH"
     gene            1561..2013
                     /locus_tag="PLV77_06405"
     CDS             1561..2013
                     /locus_tag="PLV77_06405"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNC15514.1"
                     /translation="MIEVGAKAPDFTLPDHAGQEVSLSDFSGRRVLLAFYPLDFSPVC
                     SDQLAQYKAIQGELEEAGVTLLGVSVDSAFAHKAFRDQLGIETSLLADFEPKGAVAEA
                     YGAYLGQAGHSNRSLVLIDADGVVEWVHETATPLDIPGPDLIVEAIRA"
BASE COUNT          363 a          666 c          640 g          367 t
ORIGIN      
        1 caggaagtca gcttcgaggt ggaagtgagc tcgggaacct acgtccggac cctggttgaa
       61 accctcggtg acgcctattg ctcgaagctg cgccggaccg ccgtgggtcc cctcgacatc
      121 tctctcgcgg gccgggtgct cgccccgctc gaggccctgt cgtttttgcc gcagatcgag
      181 ctcgatgcgg aacaggcccg cgcgatcggc ttcggacaga agctcgacac cggtcggttt
      241 gacctgccat cgggcattca ggcagggtcc tcaaagagtt tcgcattggt ccacggggat
      301 aacctgattg ccgtggctcg tattgaagat tcagtcctgc gccccgaagt cgtcctggtt
      361 cccgcctcgt gatcaaggta acccccctgg atgaagccga gtaccggccc cggaagatcg
      421 cgatcggaac cttcgacggc gtccacgtcg gacaccgcgc ggtaatcaag ggggccgaca
      481 ccgtgctgac cttcgagcct catcccctgg agatccttca tccggcggcc gccccgaagc
      541 tgctgatgcc cctcgacgtc cgccgcgacg ttctcgacgg tctcggagtc gaggagatga
      601 tcgtcatcca gttcgatgat tcgttcacga agatcaccgc cgagcagttc atcgaagaga
      661 tcctgatcgc ccgcctcggc gccgaagagg tgtcggtcgg agacaatttc cgcttcggcc
      721 agaaggcgaa gggcacgccg gaaatgctgg ccgccaggcc cgagttcagg acccgggtcg
      781 agcccctcgt ggaagtcgac ggcgaagccg tttcctcgac gcggattcgc gccctggtcg
      841 cagcggggga catggagctg gccagacgct gcctgggagt ccccttcatg gtcgagggcg
      901 aggtcgtgac cggtgaccag cggggtcgcg aacttggctt cccgactgcg aatatcgttc
      961 ccgatgaccg ttttgcccat cccgggcacg gtgtttacgc agccttcgcc gacggtctgc
     1021 cggcggcggt gaacatcggc gtcaggccga cctttgacac cggtcgggga gtcctgatcg
     1081 agacctacat catcgacgag gaggtcgatc tgtatggccg gatcctccgg gtcgccttcg
     1141 tcgaacggtt gcgcggcgag aaaaggttcg actcggtcga cgatctgatc gcgcagatga
     1201 aacgggacgt agagaagacg aaggccgtct gtgctagctt ccatcggccg tgaacgggtc
     1261 cgcccgtgac ccgaaaccaa ggatttttga agaaatgaca ctgaccaaag aaaacaaggc
     1321 cgaactgatc agcaagtacg gcaagtccga gggcgacacc ggctcgaccg aagtccaggt
     1381 ggccctgctt accgagcgga tcaacgatct caccggccac ctgcgggagc acgggaagga
     1441 tcaccattcc cgccgcgggc tgctgatgat ggtcggcaag cgccgccgcc tgctgcgcta
     1501 cctcgagcgg actgacgtcg agcgctaccg cgccctcgtc gccgacctcg gcctgcgcca
     1561 ttgatcgaag tcggggccaa ggctccggat ttcaccctgc ctgaccacgc cggtcaggaa
     1621 gtttccctgt cggacttttc cggccgccgg gtgctgctcg ccttctaccc gctggacttc
     1681 agcccggtct gctccgatca gctggcccag tacaaggcga ttcagggcga actcgaagag
     1741 gccggcgtca ccctgctcgg agtcagcgtc gattcggcct tcgctcacaa ggcgttccgg
     1801 gaccagctcg ggatcgaaac ctcgctgctc gctgatttcg agcccaaggg cgcggtcgcc
     1861 gaagcctacg gcgcctacct cggccaggcc ggccattcga accgttccct ggtcctgatt
     1921 gacgccgacg gcgttgtcga gtgggtccac gagacagcga cgccgctcga catcccgggt
     1981 cccgacctga ttgtcgaagc catccgggcc tagccgccat gaagccctgg atcgct
//
LOCUS       DAOQMF010000347         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37486_length_2035_cov_4.157071, whole genome shotgun sequence.
ACCESSION   DAOQMF010000347 DAOQMF010000000
VERSION     DAOQMF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37486_length_2035_cov_4.157071"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLV77_06410"
     CDS             complement(<1..188)
                     /locus_tag="PLV77_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15515.1"
                     /translation="MKETTIQDLGGTGRARVFLLAAFVALAGMLGMAGSSKAETHTIT
                     VDNGRLTMGFVFLNNKILP"
     gene            complement(255..>2035)
                     /locus_tag="PLV77_06415"
     CDS             complement(255..>2035)
                     /locus_tag="PLV77_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15516.1"
                     /translation="VALPDARQVKADYQRMVDFGPRLPGYEEHLEFCNWIEDEFVKAG
                     LELLPTDQIPYRRWRPGKMSLRVGTTDFDPATTYVRVPGTPAEGITAPMMLAGDGPVD
                     GKIVLVEAAVPTPRTAGLFIAMADYWQWRGHTAFEAAAEEFDRFAFSDWPTIKPYADQ
                     GAKAVIFISRASRAEVEGNWSPHQAEVQPIPGFVVDRDMGAELKALAAGQPEATLKLE
                     ADWKDTYVPQITGILPGESDEVLIVNSHTDGQNAFEENGAVALVHLARHFASLRGSRK
                     LKRTLVFIAWPGHMANVSNRPDDRTTLPEQFGWISSHRDLFDRTVAAVTIEHLGATRW
                     SDDPVEGYHSTGMSDFYAIWTTLGAMQDLCKVALKESDLQRHALLRPPIQVTPGVAWH
                     HRGIPHVAGISAPKYLVRVSENGDIDKLDASLASRQTGFYADVVKSLDSADPAALKAG
                     GEGDSSFNSVSDREYPMTRARSGPAREVVLDAGDGGRLAIYIGGRAAGGRDIGVQMAA
                     LDHSLERVTVAVYRDDTLLGKSRPTRIDRKVKRVAIHRSGHRADHRNLHRNRSAARFR
                     DGTYTFVVRQDGKLLGQRNVTYQRRR"
BASE COUNT          310 a          653 c          684 g          388 t
ORIGIN      
        1 gggaggatct tgttgttgag gaagacgaaa cccatcgtca agcgtccgtt gtcaaccgtg
       61 atcgtgtggg tctcggcctt tgatgaaccg gccatgccaa gcatgccggc cagcgcgaca
      121 aaggcggcaa gcagaaacac cctcgccctt cctgtccctc ccaggtcctg aatagttgtc
      181 tccttcattg cctctccttg ttgctgttca tgtgctgcgc agtcatcgca gtcctgtttc
      241 cctcgtccgg gtacctagcg gcgccgctgg taggtgacgt tccgctgacc cagaagcttg
      301 ccgtcctgac ggacgacgaa tgtgtaggtg ccgtcgcgga atctggccgc gctccggttc
      361 ctgtgaagat tgcggtggtc ggcccggtgt ccgctccggt ggatcgcgac ccgcttgact
      421 ttgcggtcga tgcgggtcgg tctggatttg ccgaggaggg tgtcgtcgcg gtagaccgcc
      481 accgtgaccc gctcgagcga gtggtcgagt gctgccatct ggacgccgat gtcccggccg
      541 ccggcagcgc ggcctccgat gtagatcgcg aggcgaccgc cgtcgcccgc gtccagcacc
      601 acttcccgtg ccggccccga gcgggccctg gtcatcggat actcgcgatc ggacacgctg
      661 ttgaagcttg agtcgccttc accacccgcc ttgagggccg cagggtcggc cgagtccagc
      721 gacttgacca cgtcggcgta gaagccggtc tgccgggagg ccagggaggc gtcaagcttg
      781 tcgatgtcgc cgttctcgga aaccctgacc aggtacttgg gcgccgagat cccggccacg
      841 tgcgggatcc cccggtggtg ccaggcgacg ccgggcgtaa cctggatcgg tggcctgagc
      901 aacgcgtggc gctgcagatc cgactccttc agggcgacct tgcacaggtc ctgcatggct
      961 cccagggtgg tccagatcgc gtagaaatcg gacattccgg tcgagtggta gccctcgacc
     1021 gggtcgtcgg accagcgtgt ggccccgagg tgttcgatcg tgaccgcggc gacggtgcgg
     1081 tcaaagagat cccggtgcga gctgatccag ccgaactgct ccggcagcgt tgtccggtca
     1141 tccggtcgat tgctgacatt ggccatgtgg ccgggccagg cgatgaagac gagcgtgcgc
     1201 ttcagtttcc ggctgccgcg aagcgaagcg aaatgcctgg ccagatggac gagggccaca
     1261 gccccgtttt cctcgaaggc gttctggccg tcggtatgcg agttgacgat cagcacctcg
     1321 tcgctctcgc ccggcaggat gcccgtgatc tgcggaacgt aggtgtcctt ccagtccgct
     1381 tccagcttca gggtcgcctc gggctggccg gcggcgagcg ccttcagttc ggcccccatg
     1441 tcgcggtcaa ccacgaagcc gggaatcggc tggacttccg cctggtgagg tgaccagttg
     1501 ccttcgactt cggcccgcga agcccggctt atgaagatca ccgccttggc gccctggtcg
     1561 gcgtagggct tgatcgtggg ccagtccgag aaggcgaaac ggtcgaattc ctcggccgcc
     1621 gcttcgaagg ccgtgtgtcc acgccactgc cagtagtcgg ccatcgcgat gaagaggccg
     1681 gcggtgcgcg gggtcggcac cgcggcctcg acaaggacga tcttcccgtc gaccgggccg
     1741 tcgcctgcca gcatcatggg ggccgtgatc ccctccgccg gagtgccggg aacccgcacg
     1801 taggtggtcg cgggatcaaa gtcggtggtg ccgacccgca gcgacatctt gcccggccgc
     1861 cagcggcggt aggggatctg gtcggtgggc agcagctcga gccctgcctt tacgaactcg
     1921 tcctcgatcc agttgcagaa ttcgagatgt tcttcgtagc cggggagccg ggggccgaaa
     1981 tcgaccatcc gctgatagtc ggccttgacc tgccttgcat cgggcagggc tacct
//
LOCUS       DAOQMF010000348         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37525_length_2034_cov_4.066700, whole genome shotgun sequence.
ACCESSION   DAOQMF010000348 DAOQMF010000000
VERSION     DAOQMF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37525_length_2034_cov_4.066700"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLV77_06420"
     CDS             <1..1072
                     /locus_tag="PLV77_06420"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNC15517.1"
                     /translation="EVNAKMESFDCTAAGRAIAAYVEELSNWYVRLSRRRFWEGDAAA
                     FATLRHCLVEVSALLAPFTPFIAEEIHQNLAGGADGDFGSLPDSVHLRDFPSPAAGLR
                     DQELESAMAATRQTVELGRASRAQAKAKVRQPLARAVVVATDAERDAISTQTDLIKSE
                     LNVKELEFVTDQSDLVSYLVKPNFRTLGPRFGKSMPQVSAAVAGLDPAHVAETVESGG
                     EIGISIEGKDHTLTPEDLLLSMEPLEGYQVEAESGHAVALALELDDELIREGLAREIV
                     HAVQNARKDAGLEITDRIGLSLAGDQELLDAAATHEGYVTGEVLARTIELGGDTAEAA
                     SPGTPEVTIDGRALKISVTRVD"
     gene            1110..1709
                     /locus_tag="PLV77_06425"
     CDS             1110..1709
                     /locus_tag="PLV77_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNC15518.1"
                     /translation="MASARPRAKTQAERRAETRAALLDATVESLVTYGYTGTTTGRIA
                     ELAGVSRGAQTPYFRSRADLLSAAVTHLAELRLAAVRERFSGKAITVEEGLDALWEEH
                     QGDAFDALLELWIASRTDDEMREHLVRIERDVGVAIVQESARALGPIADRPDFNDDMI
                     YILATMRGLSMMRISHGVSDRTLNRRWSEIRERLAQVLT"
     gene            complement(1711..>2034)
                     /locus_tag="PLV77_06430"
     CDS             complement(1711..>2034)
                     /locus_tag="PLV77_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC15519.1"
                     /translation="RPIANFQHNRFTLAELRTEIEIGRCFIDRCIERYVDGTCTVEEA
                     AMAKWWTTELVSKVTDAGVQLHGGYGYTTEYRIGKAWVDARVGRIYAGTNEIMKELIG
                     KTMGL"
BASE COUNT          347 a          698 c          667 g          322 t
ORIGIN      
        1 ggaagtcaac gcgaagatgg aaagcttcga ctgcaccgcc gccggccggg cgatcgcggc
       61 ctacgtcgag gaactctcca actggtatgt gcggctttcc cgtcgccgct tctgggaggg
      121 cgatgccgcc gccttcgcaa ccctgaggca ttgcctggtc gaggtgtcgg ccctgctcgc
      181 ccccttcacc ccgttcatcg ccgaggagat tcaccagaac ctggccggcg gggccgatgg
      241 cgatttcggg tcgctgcccg actcggtcca cctgcgcgac ttcccctcgc cggcggcagg
      301 actccgggac caggaactcg agtcggcgat ggcggctacc cgacagacgg tcgagctcgg
      361 ccgggcctcg cgggcccagg ccaaggcgaa ggtccgccag ccgctggccc gggcggtcgt
      421 cgttgcgacc gacgccgagc gggacgcgat ctcgacccag accgatctga tcaagagcga
      481 actgaacgtg aaggaactgg agttcgtgac cgaccagtcg gatctggtca gctatctggt
      541 caagccgaac ttccgcaccc tcggcccgcg cttcggcaag tcgatgccgc aggtttccgc
      601 tgcggtcgcc gggctcgatc cggcccacgt ggccgagacg gtcgagtcgg gcggcgagat
      661 cgggatctcg atcgaaggca aggaccacac gctcaccccg gaggacctgc tgctcagcat
      721 ggaaccgctg gagggctatc aggtggaagc cgagtcgggc cacgcagtcg ccctcgccct
      781 cgagctcgac gacgagctga tccgcgaggg cctggcccgc gagatcgtcc acgccgtcca
      841 gaacgcccgc aaggatgccg gcctcgagat caccgaccgg atcggccttt cactggccgg
      901 cgaccaggaa cttctcgatg ccgccgccac ccatgagggc tacgtgaccg gagaagttct
      961 ggcccggacg atcgaactcg gcggcgacac ggccgaggcg gccagccctg gaacccccga
     1021 agtgacgatc gacggccggg cgctgaagat ctcggtcacc cgggtcgact gagaatcgcc
     1081 ggccaacgcc gggccgatat cttcacccaa tggcttccgc tcgtccccgc gcgaaaaccc
     1141 aggctgaacg ccgcgcggag acacgcgcgg cgctgctgga tgcgaccgtc gaatcgctgg
     1201 tcacttacgg gtacaccggg accaccaccg gccggatcgc cgaactggcc ggcgtttccc
     1261 gcggggcaca gaccccctat ttccgcagcc gggcggacct gctcagtgcc gccgtgaccc
     1321 atctcgccga gttgaggctg gccgccgtgc gggaacggtt cagcggcaag gcgatcaccg
     1381 tcgaggaagg cctcgacgcg ctctgggagg agcatcaggg tgacgcgttc gacgccctgc
     1441 ttgaactgtg gatcgcttca cggaccgacg atgaaatgcg cgagcacctg gtcaggatcg
     1501 aaagggacgt cggggtggca atcgttcagg agtccgcgag ggccctcggt ccgatcgccg
     1561 accggcccga cttcaacgac gacatgatct acatcctcgc caccatgcgc ggcctgtcca
     1621 tgatgaggat cagccacggt gtaagcgacc ggaccctgaa ccgacgctgg agcgagatcc
     1681 gggaacggct ggctcaggtt ctgacctgag tcagaggccc atcgtcttgc cgatcagttc
     1741 cttcatgatc tcgttggttc cggcgtagat ccggccgacc cgggcgtcca cccaggcctt
     1801 gccgatgcgg tactcggtcg tgtacccgta accaccgtgg agctggactc cggcgtccgt
     1861 caccttgctg accagttcgg tggtccacca cttggccatc gccgcctcct cgaccgtgca
     1921 ggtcccgtcc acgtaccgct cgatgcaccg gtcgatgaaa cagcggccga tctcgatctc
     1981 ggtccgcagt tcggccaggg tgaagcggtt gtgctggaag ttggcgatcg gccg
//
LOCUS       DAOQMF010000349         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37624_length_2031_cov_3.911943, whole genome shotgun sequence.
ACCESSION   DAOQMF010000349 DAOQMF010000000
VERSION     DAOQMF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37624_length_2031_cov_3.911943"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1237)
                     /locus_tag="PLV77_06435"
     CDS             complement(56..1237)
                     /locus_tag="PLV77_06435"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNC15520.1"
                     /translation="MGGLDLGLERAGVRIVGQCEADPAARRVLERHWPGVPLSEDVRT
                     VNAEWCESNGLELPESRGIGAGADARSEHGNGNGKGRGDRVQLADRNRSTSTVKRGVA
                     VASQGTDPSGIDLVCGGFPCQDLSVAGKRAGLDGERSGLFFEAARVADLVLGDGGWLI
                     IENVPGLLSSNGGRDFGIVLATLADLGFYDLAWRVLDSRYFGVPQRRRRVFIVARRAR
                     GDRAKQVLLEPESGGGDFAPGQETGQGTAGTFGVRADSLSHALTTRSAVEEDGTGRGN
                     PIVTANALDRQAGGPDDNSAQANHLVVAKCVTTSQERQDFDTQDFVHPGLRSDQSSVR
                     RLTPTECERLQGFPDGWTIPDGTDWREYPRPDGPRYAQMGNAVTVPVAEWIGRRLMEY
                     N"
     gene            complement(1358..1705)
                     /locus_tag="PLV77_06440"
     CDS             complement(1358..1705)
                     /locus_tag="PLV77_06440"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNC15521.1"
                     /translation="MVDQILAGDKTMTRRPVKDDLPCRYAVGNTYSVQPGRGMKGVAR
                     IRVLAVRRERWFEITEEDAMAEGFRDGYVSVGDREIWRGARAQFFAYVWALYPGLDLN
                     AECWVIEFELVAA"
     gene            1801..>2031
                     /locus_tag="PLV77_06445"
     CDS             1801..>2031
                     /locus_tag="PLV77_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15522.1"
                     /translation="MFSGKPTHRLDLNLNIALELGFSFGASGLAQTFPDMAQTHAPAR
                     EVWAVLAALNATGSASCFMGRGRDGGWLPGFSP"
BASE COUNT          390 a          676 c          555 g          410 t
ORIGIN      
        1 ttctactgaa tagcccacca gggggccagg tttgaactga tctaccctca tgggtctaat
       61 tgtattccat gagcctgcgt ccgatccact cagccaccgg aaccgtcacc gcgttaccca
      121 tctgcgcgta cctcgggcca tccggtcgcg gatattcccg ccagtccgtg ccatcaggaa
      181 tggtccagcc gtcgggaaat ccttgcaggc gctcgcactc ggtcggggtc aggcgccgga
      241 cactggactg atctgaacga agtcctgggt gaacgaagtc ctgggtgtcg aagtcctggc
      301 gttcctgcga ggttgtgaca catttcgcca ccaccaaatg atttgcctga gcggagttat
      361 cgtccgggcc gccggcctgc ctgtcaagtg cgttggcggt cacgattggg tttccccgtc
      421 cggttccatc ttcctcaacg gctgatcggg ttgtgagcgc gtggctaagg ctgtcagcgc
      481 ggactccaaa ggttccggca gtcccctgcc ccgtttcctg gcccggcgca aaatccccgc
      541 cgccgctttc gggctcaaga agaacctgct tggcacggtc gcctctagca cgtcgggcaa
      601 cgatgaaaac acgccgcctc cgctggggca ctccgaagta tcggctgtcc aacactctcc
      661 aggcgaggtc ataaaacccg aggtcggcca gcgtcgcgag aacgattccg aaatcccttc
      721 caccgtttga ggaaagaagt ccgggaacat tctcaatgat gagccatcct ccgtctccga
      781 gaacaaggtc agcgactctg gctgcctcga agaacaatcc tgatcgttca ccgtcgaggc
      841 cggcgcgttt tccggcaact gagaggtctt ggcaggggaa tcctccgcat acgaggtcaa
      901 taccgctggg gtctgtccct tgtgaagcga cggcgacacc tcgcttgacg gttgaagttg
      961 atctgttccg gtctgccaat tgaacgcgat caccccggcc cttcccgttg ccattcccgt
     1021 gttctgaccg agcgtcggca ccagctccga ttccgcgtga ttcagggagt tccaggccgt
     1081 tgctttcaca ccattcggca ttgacggttc ggacatcctc tgacaacggg actccgggcc
     1141 agtgtcgttc aaggactcgt cgtgctgctg ggtcggcttc gcactgtccg acgattctga
     1201 ccccggcccg ttcaagtccg agatccagtc cgcccacgcc tgaaaaaagg gagaggccat
     1261 tcaccacgcc ctccgttcgc aaaccgggcc tgccagtaga aggcaagtga gagggatagg
     1321 ggcgagcttc atgcggcagc ctcaacctga gcgtccgtca tgcggccacc aactcgaact
     1381 cgatcaccca acactccgcg ttcaaatcaa gacccgggta aagagcccac acgtacgcga
     1441 agaactgcgc tcgcgccccg cgccagatct ctcgatctcc taccgagaca tacccgtccc
     1501 ggaaaccctc agccatcgca tcttcctcag tgatttcaaa ccaccgttcc cgccgaaccg
     1561 ccagcaccct tatccgggca acccctttca tgccccggcc cggttgaacc gagtaggtgt
     1621 tgcccaccgc gtaacggcag ggaaggtcat ccttcaccgg cctccgggtc atcgtcttat
     1681 cccccgccag gatctgatcg accatgactt gcttgaaaat cactcccgtt cctcccccat
     1741 agacgaaagg gaatcccggg ccgaagtctt ggttgagggt cggctcaccg cctcccggtt
     1801 ttgttcagtg gcaaaccgac gcatcgcctc gacctgaacc tcaacatcgc gctcgaactc
     1861 ggcttcagct ttggcgcgag cggcctcgcg caaaccttcc ctgatatggc tcagacacat
     1921 gctcccgccc gggaagtgtg ggcggttctc gcagccctca acgcgacagg ttccgcatcc
     1981 tgtttcatgg ggagaggaag ggacggagga tggttgcccg gattcagccc t
//
LOCUS       DAOQMF010000350         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37714_length_2029_cov_4.335360, whole genome shotgun sequence.
ACCESSION   DAOQMF010000350 DAOQMF010000000
VERSION     DAOQMF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37714_length_2029_cov_4.335360"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PLV77_06450"
     CDS             <1..1006
                     /locus_tag="PLV77_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC15523.1"
                     /translation="ERIKFAPYIIYAAVFGAVIYPMVGHSVWGGGLLTDIGGKSVMDF
                     AGSSVVHLTGAVGGFVALLLLGARKGKYAADGSPRAIPGHSMPLVGLGIIILWIGWFG
                     FNGGSTLGTDLSFFGEVMWNTQIAAAAGVIGAMLFTYMKTKSLDVGMAGNGAIAGLVA
                     ITAPCGFVETWTAPIIGFIAGGVVVFGVLTIEKYLDDPIGALSAHGLAGIWGTLAVGI
                     FASPRLTISAPGIWYGIFGDADFGATLGQLGVQALAVVATFTFVFAASYLVFGIIKAT
                     IGLRVTEAEEDAGLDIASHGMYGYPEAFIPQPELPVGEYTPSMAGTPVASSAESPSQA
                     "
     gene            1022..1378
                     /locus_tag="PLV77_06455"
     CDS             1022..1378
                     /locus_tag="PLV77_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNC15524.1"
                     /translation="MKKIEAFIRHEAFEPIRTDLLEKGIPSMSILEAKGSGRQKGIVE
                     TYRGSTVTVNVRPKLKIEIVVADSEKDLVVETILRHARSGEIGDGKIFVIPVEESIRI
                     RTGERGDEVTQVHLDD"
     gene            1496..>2029
                     /locus_tag="PLV77_06460"
     CDS             1496..>2029
                     /locus_tag="PLV77_06460"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNC15525.1"
                     /translation="MADRSRIGVLVTGGTVGSRSEPLSGREKLVRLSESDRQLDVFSG
                     PNVTAVLEKAGISPVLRQPVNMLSENMTPEHWVIMGREIERLIEEEDPDGILVLHGTD
                     TMSFTSTALSFMLAGLKVPVVLTGSNRPPNEPDTDADTNIADALIALQHLSPGVYLSF
                     AGRPNAKSRVHDGVSVRK"
BASE COUNT          362 a          659 c          641 g          367 t
ORIGIN      
        1 ggagcgcatc aagttcgctc cgtacatcat ctacgccgca gtcttcggag ccgtgatcta
       61 cccgatggtc ggtcactcgg tctggggcgg cggcctgctc accgacatcg gtggcaagtc
      121 ggtgatggac ttcgccggtt cctcggtggt ccacctcacc ggagcggtcg gcggattcgt
      181 cgcactgctc ctgctgggtg ccagaaaggg caagtacgcg gccgacggat caccccgcgc
      241 gatccccgga cactcgatgc cgctggtcgg cctcgggatc atcatcctgt ggatcggctg
      301 gttcggattc aacggcggct cgaccctggg caccgatctc tccttcttcg gcgaggtcat
      361 gtggaacacc cagatcgctg ctgcggcagg cgtgatcggc gcgatgctct tcacctacat
      421 gaagaccaag tcgcttgacg tcggcatggc cggcaacggc gcgatcgccg gcctcgtcgc
      481 gatcaccgcg ccctgcggtt tcgtcgagac ctggacggcc ccgatcatcg gcttcatcgc
      541 cggtggcgtc gtggtcttcg gcgtcctgac gatcgagaag tacctcgacg atccgatcgg
      601 tgccctctcg gcccacggtc tggccggcat ctggggaacc ctcgcggtcg gcatcttcgc
      661 ctcgccgcga ctgaccatct ccgcgccggg catctggtac ggaatcttcg gtgacgccga
      721 cttcggcgcg accctgggtc agctcggggt tcaggcgctg gccgtcgttg cgacgttcac
      781 attcgtgttc gccgcttcgt accttgtgtt cggaatcatc aaggcaacca tcggcctccg
      841 tgtcacggaa gcggaagagg atgccggtct cgacatcgcc tcgcacggca tgtacgggta
      901 cccggaagcc ttcatcccgc agccggagct gccggttggt gaatacaccc cgtcgatggc
      961 agggacaccg gtcgcatcgt cggccgagag tcccagccag gcctaggaag gagatgctca
     1021 gatgaaaaag atcgaagcat tcattcgcca cgaggcgttc gagccgatcc ggacggacct
     1081 cctcgaaaaa ggaatcccgt caatgtcgat tctcgaagcc aagggctcgg gacggcagaa
     1141 ggggatcgtc gagacctacc ggggatcgac agtcacggtc aacgtccggc cgaagctcaa
     1201 gatcgagatc gtcgtggccg attccgaaaa agacctcgtc gtcgaaacga tcctgcgtca
     1261 cgctcgatcc ggagagatcg gagacggcaa gatcttcgta atcccggtcg aggagtcgat
     1321 caggatcagg accggcgagc gaggagacga agtaacccag gtccatctcg acgactagcg
     1381 atgggctgac acggatcggg cggcggggcc tcgtgccccg ccgcccttgt ccgttgtcgg
     1441 gactgatttg actacagccg ccaaccgcat tccggccgcc agatacgctg gctccgtggc
     1501 cgaccggagc agaatcggag tcctggtgac cgggggtacc gtgggctccc gctcggagcc
     1561 cctttcgggc cgggaaaagc tggtcaggct cagcgagtcc gaccggcagc ttgatgtgtt
     1621 ctccggtccc aacgtgaccg ccgttctcga aaaggcgggc atttcaccgg ttctccggca
     1681 gccggtcaac atgttgtccg agaacatgac gcccgaacac tgggtgatca tgggccggga
     1741 gatcgagcgc ctgatcgagg aagaagatcc ggacgggatc ctcgtcctgc atggcaccga
     1801 cacgatgtcc ttcacctcga cggcactctc attcatgctc gccggcctca aggtcccggt
     1861 ggtgctgacc gggtcaaacc ggccgccgaa cgagcccgat accgacgccg acaccaacat
     1921 cgcggacgcc ctgatcgcgc ttcagcatct ctcgcccggg gtctacctct cctttgccgg
     1981 ccggccgaat gcaaagagcc gggtgcacga cggggtgtcc gtccgcaag
//
LOCUS       DAOQMF010000351         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37716_length_2029_cov_3.747720, whole genome shotgun sequence.
ACCESSION   DAOQMF010000351 DAOQMF010000000
VERSION     DAOQMF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37716_length_2029_cov_3.747720"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLV77_06465"
     CDS             complement(<1..631)
                     /locus_tag="PLV77_06465"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNC15526.1"
                     /translation="MPGLVIVGTQWGDEGKGKVTDLLAKDASAIVRFQGGNNAGHTIV
                     HDGEEYKFHLIPSGILHEDKYCVIGNGVVVDPRVLLEEIEGLHRAGVSASNLRVSSNA
                     HLIMPYHVLVDAAEELKLGKLSIGTTRRGIGPAYADKAYRIGIRVQDLLDEKILRKKI
                     MAALEHKKKSLQELAVRRRKLHKEAEPDEEIQIDDRLDLHTITEHYLSYG"
     gene            complement(650..1387)
                     /locus_tag="PLV77_06470"
     CDS             complement(650..1387)
                     /locus_tag="PLV77_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC15527.1"
                     /translation="MAAGEFEASGNGQPPCPYGACDGNGWILNEDGSATECRCRQPRL
                     AKARASGVNSVLPKRYRGVSFDRPPVTEIHPGAVTVVRDWIERLDENLDQGNGLWLMG
                     DTGTGKTTLAMLVSKEALRRDHTVAIYSMPSLLTRIRATYGAEAGEESYDQFFERLCE
                     VDLLHIDDLGAEKQTEWVLEQLYALVNERYEREKSIVVTTNITDQGELEQQIGPRTVS
                     RLTEMCGESVVPLFGEDARQKPQLRDG"
     gene            complement(1387..>2029)
                     /locus_tag="PLV77_06475"
     CDS             complement(1387..>2029)
                     /locus_tag="PLV77_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNC15528.1"
                     /translation="RKARFKGGDRTWKKINKSLNALANVPLFVDVTSDLGLLDLRAKA
                     RRLHEQEIRRRAGTDRENEGLSLIIVDYLQLMRMDDRIANRAEQVGQVSRGLKLLASE
                     LKVPVVALSQLNRANESRTDKRPMLSDLRESGNIEQDADLVLFIYREDVYQKDREKHT
                     GKAEVIVAKNRSGPIGTSHLIFFPHIPKFAEESREHMAGEGSPQAVQAEADAI"
BASE COUNT          291 a          649 c          663 g          426 t
ORIGIN      
        1 ggccgtagct caggtagtgc tcggtgatcg tgtgcaggtc gagccggtca tcgatctgga
       61 tctcctcgtc cggctcggcc tccttgtgca acttgcgccg cctgaccgcc agctcctgga
      121 gcgacttctt cttgtgctcg agggcggcca tgatcttctt gcggaggatc ttctcgtcga
      181 gcaggtcctg gacccggatc ccgatccggt aggccttgtc ggcgtaggcc gggccgatcc
      241 cgcggcgggt ggtgccgatc gagagcttgc ccagcttgag ttcctcggcc gcatcgacta
      301 gcacgtggta gggcatgatc aggtgggcgt tcgatgaaac ccgcaggttc gaggcggaga
      361 cgccggcccg atgcagcccc tcgatttcct ccagcagaac ccggggatcg acgaccacgc
      421 cgttgccgat cacgcagtac ttgtcctcgt gcaggatgcc ggaagggatc aggtgaaact
      481 tgtactcctc gccgtcgtgc acgattgtgt ggccggcgtt gttgccgccc tggaagcgca
      541 cgatcgcgga ggcgtccttg gccagcaggt cggtgacctt gcccttgcct tcatcgcccc
      601 actgggttcc gactattacg agtcctggca tgaagagggc agtctagctt cagccgtccc
      661 ggagttgcgg cttctgccgg gcgtcctcgc cgaatagcgg gaccacgctt tcaccgcaca
      721 tctcggtcag ccgcgaaacc gttcgggggc cgatctgctg ctcgagttcg ccctggtcgg
      781 tgatgttggt ggtgaccacg atcgacttct cgcgctcgta gcgttcgttg accagggcgt
      841 agagctgttc gagcacccat tcggtctgct tttcggcgcc gaggtcatcg atgtggagca
      901 ggtcgacctc gcagagtcgc tcgaagaact ggtcgtagga ctcttcgccg gcctccgccc
      961 cgtaggtcgc ccgaatccgg gtaagcaggc tgggcatcga gtagatcgcg accgtgtgat
     1021 cccgcctgag cgcttccttc gagaccagca tggcgagggt ggtcttgcct gtgccggtgt
     1081 cccccatcag ccagagtccg ttgccctggt caaggttctc gtcgagccgt tcgatccagt
     1141 cccggacgac ggttaccgca ccggggtgga tctcggtcac cggcggacgg tcgaaggaaa
     1201 cgccgcggta gcgcttcggc aggaccgagt tgactccgga agcgcgggcc ttcgccagcc
     1261 ggggctgccg gcacctgcat tcggttgccg agccgtcctc gttcagaatc cagccgttgc
     1321 cgtcacaggc gccatagggg cagggcggct ggccgttgcc ggatgcctcg aattcgccgg
     1381 ccgccatcag atcgcgtccg cttccgcctg gaccgcctgc ggcgagccct cgccggccat
     1441 gtgctcacgg ctttcctcgg cgaatttcgg gatgtgcggg aagaagatca ggtggctggt
     1501 gccgatcggg ccgctccggt tcttggcgac gatcacctcc gcctttccgg tgtgcttttc
     1561 ccggtccttc tggtagacgt cttcgcggta gatgaagagg accaggtcgg cgtcctgttc
     1621 gatgttgccc gactcgcgca ggtcggagag catcggccgt ttgtcggtcc ggctttcgtt
     1681 cgcccggttg agctgcgaaa gcgcgacgac cggcactttc agttcgctgg cgagaagctt
     1741 gaggccacgg ctgacctgcc cgacctgctc ggcccggttg gcaatccggt catccatccg
     1801 catcagctgc aggtagtcga cgatgatcag tgaaaggcct tcgttttcac ggtccgtgcc
     1861 cgcacgccgg cggatctcct gctcatgcag tcgccgcgcc ttggcccgca gatcgagcag
     1921 ccccaggtcc gaggtgacgt caacgaacag gggcacgttg gccagggcgt tcagggactt
     1981 gttgatcttc ttccaggtcc ggtcgccccc cttgaatctg gctttccgg
//
LOCUS       DAOQMF010000352         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37731_length_2029_cov_2.763425, whole genome shotgun sequence.
ACCESSION   DAOQMF010000352 DAOQMF010000000
VERSION     DAOQMF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37731_length_2029_cov_2.763425"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLV77_06480"
     CDS             complement(<1..584)
                     /locus_tag="PLV77_06480"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC15529.1"
                     /translation="MNWRTSIVVVGVVALLGFLVWGLVKKGDSSLAVGEPVPVATLPT
                     LPEGGEGSIEDYRGQWVLMNVWASWCGPCKAESPALDRFEEKNRDRIVVLGIDTKDLS
                     EDAMNFIREFNIKYPQLRDASGDYTDQKLKTTGVPESFLIDPEGNLVAHFPGPFEDEA
                     AIEEFAAPALESEANVDPAGVTGQNGPAGGSAKS"
     gene            692..764
                     /locus_tag="PLV77_06485"
     tRNA            692..764
                     /locus_tag="PLV77_06485"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:725..727,aa:Gly,seq:ccc)
     gene            799..1812
                     /locus_tag="PLV77_06490"
     CDS             799..1812
                     /locus_tag="PLV77_06490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC15530.1"
                     /translation="MIVAYESGPELGRTLPGLAGELAQGDEVIVVDNGSKVSPRDVVA
                     EHLPAATVIEMGENAGFTAATNRGAAASSGDLVLMLNPDAMPEPGFGEAIRRPFAAQP
                     DWGAWMALVACRIDGQKVVNSWSNPVHFTGICWAGGHGEPLERVGGTREIGVASGAAL
                     AVRKEVWNRVGGLSEDFFLYQEDTDISLRIQAVGHRIGLQAEAVVDHDYDFHSNDRKW
                     FWLERNRLATVIRNYPAPLLALVAPALLGTELVLLAVSAKQGWLGAKLKSYRDLIRWL
                     PRLRRERAQIQSARVIPASQFADLLTPDLDSPLIPEFARKGLVRAMLRTYWRVVRTLL
                     ARR"
BASE COUNT          333 a          638 c          684 g          374 t
ORIGIN      
        1 tctgacttgg ctgaccctcc cgccgggccg ttttgcccgg tgacgccggc cgggtccacg
       61 ttcgcctctg actcgagggc gggtgcggcg aactcttcga tcgccgcttc gtcctcgaag
      121 gggccgggga agtgagcgac cagattccct tcggggtcga tcaggaagga ttcgggcacc
      181 ccggtggtct tcagcttctg gtcggtgtag tcgccgctgg cgtcacgaag ctgcgggtac
      241 ttgatgttga actccctgat gaagttcatc gcgtcctcgc tcaggtcctt ggtgtcgatc
      301 ccgagcacga cgatccggtc gcggttcttc tcttcgaatc ggtcgagcgc cggcgactct
      361 gccttgcacg ggccgcacca ggaggcccag acgttcatca acacccactg gccgcggtag
      421 tcctcgatcg agccttcgcc cccctcgggc agggtcggca gggtggcaac cggcacgggt
      481 tcgcccacgg caaggctcga gtcacctttc ttgaccagtc cccagacgag gaagccgagc
      541 agcgcgacaa cgccgaccac gacgatggaa gttcgccagt tcacgatggt cgattatgga
      601 gaaccgggcc cggcggccgc cccgcgccca cgctgaacca ccaccggcgg gcggagtcaa
      661 tctgtgacgg ggtaaggttc caagccgctt cgcggacgta gctcagttgg tagagcgcga
      721 gcttcccaag ctcgaggtcg cgggttcgag acccgtcgtc cgcttttgac cgaacaccgc
      781 ctaccgactc tctcggtcgt gatcgtggcc tacgaatccg ggcccgaact ggggcggacg
      841 ctgcccggac tggccggtga actggctcag ggggatgagg tgatcgtggt cgacaatggt
      901 tcgaaggtct cgccgcggga tgtcgtggcc gagcatctgc cagcagcgac ggttatagag
      961 atgggcgaaa acgccggttt caccgcggcg accaaccgcg gggctgccgc ttcaagcggg
     1021 gatctggtgc tgatgctgaa ccccgacgcc atgcccgagc ccggctttgg cgaggcgatc
     1081 cgccggccct tcgctgcgca accggactgg ggcgcctgga tggcgttggt ggcctgtcgg
     1141 atcgacgggc agaaagtcgt gaattcctgg tccaacccgg ttcatttcac gggcatttgc
     1201 tgggccggcg ggcacgggga gccgctcgaa cgcgtgggag ggacacgcga gataggtgtt
     1261 gcatccggcg ctgcgttggc ggtgaggaag gaagtgtgga accgggtcgg cggcttgtcc
     1321 gaagacttct tcctctacca ggaagacacc gacatttccc tgcgcattca ggccgtcggg
     1381 cacaggatcg gtttgcaggc cgaagcggta gtcgaccacg actacgactt tcattccaac
     1441 gaccggaagt ggttctggct cgagcgaaac cggctcgcga cggtgatccg caactacccg
     1501 gccccgctgc tcgccctggt tgccccggcc ctgctcggca ccgaactggt gctattggcg
     1561 gtcagcgcga aacaggggtg gctcggggcc aagctgaagt cctaccgcga cctgatccgc
     1621 tggcttcccc gcctgaggcg ggagcgggcg cagatccagt cggcccgggt gatcccggcc
     1681 agccagttcg ccgacctcct gacacccgac ctcgattcac ccctgattcc cgaattcgcc
     1741 cgaaaagggt tggtgagggc aatgctccgc acctactggc gagttgtccg gaccctcctc
     1801 gccaggaggt agccatggag cccgatcatc acttgttgat gatcgtgatc gggcgggtgc
     1861 ctgctcgaag tccggatccc caaccggtgt gaaagctgtg ggagcgccga aggcgcgact
     1921 gcagttaagt cgcctgggag atcgggcgga tcaggatctc gttcaggtcg acgccttcag
     1981 gctggtcgag ggcgaactgg atcgtgcggg cgatgtcttc gtccttgag
//
LOCUS       DAOQMF010000353         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37830_length_2026_cov_3.086251, whole genome shotgun sequence.
ACCESSION   DAOQMF010000353 DAOQMF010000000
VERSION     DAOQMF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37830_length_2026_cov_3.086251"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..323)
                     /locus_tag="PLV77_06495"
     CDS             complement(15..323)
                     /locus_tag="PLV77_06495"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC15531.1"
                     /translation="MDEVFKALADPTRRGLLDSLFERDGQTQKELEEGLEMTRFGVMK
                     HLKVLEEAGLVVTRRRGREKLHFLNPVPIRQVHDRWVSKFSEPWAAGLGELKRNLEKE
                     "
     gene            complement(391..1143)
                     /locus_tag="PLV77_06500"
     CDS             complement(391..1143)
                     /locus_tag="PLV77_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15532.1"
                     /translation="MSFLRPLTRVESWSVDGTPTKASCGNGTSSHEAPDADCECGLYA
                     YHPWSGMGQHLVEDSGHRDGAVVGLVEAWGRVEVHSEGFRAEYAKPVAFFLATSDSWD
                     DHFTQGYIRVLRKLASGCGAEIIDTRRRGAIGAWLENHPGQLDPDAVRRLLPGVQVGP
                     VRIRPRHHALDLIGTGIERLIKGIGILLYGIFMTVSISVQLIWWGFLLYVIFAAVTGL
                     DPLGIGSHLGPDISDPTHPQQDSPTMTPGQLR"
     gene            complement(1254..1706)
                     /locus_tag="PLV77_06505"
     CDS             complement(1254..1706)
                     /locus_tag="PLV77_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15533.1"
                     /translation="MRHDNRYHRVPGKECTCGLYAHHAYRFPPQILRRLHRPDPRTVV
                     GFVEAWGRIELHLEGFRAEFARPVAFINFTWPKPKEDEDPRWYGTRKRRCAYEYRLIE
                     LAERCGAEVIDCNKGDKLGAWIHDDPRALRRETLARLIPRRRRSPRRS"
BASE COUNT          345 a          655 c          647 g          379 t
ORIGIN      
        1 gcgtttccat cgtcctactc cttttcgagg tttcgtttga gctcgcccag gccggcggcc
       61 cagggctcgg agaacttgct gacccagcgg tcatggacct gccggatcgg gacggggttg
      121 aggaagtgga gcttttcccg gccgcgcctc ctggtcacga ccagccctgc ctcttccaga
      181 accttgaggt gcttcatcac gccgaagcgg gtcatctcca gaccttcctc cagctccttc
      241 tgggtctgcc cgtcccgctc gaagagcgag tcgagcaatc ccctccgggt cggatcagcc
      301 aaagccttga acacctcgtc catgcccggg ataataggtg actaattagt cacatgtcaa
      361 gtgtcccgca cggcggccac cgattaccag ctatcgcagc tgcccgggcg tcatcgtcgg
      421 agaatcctgc tgtgggtggg tcgggtcgct gatgtcaggc ccgaggtgcg agccgatccc
      481 cagcggatcg agtcccgtca cggcggcgaa gatcacatac aggaggaagc cccaccagat
      541 cagctggacg gagatcgaga ccgtcatgaa gattccgtag agcaggatgc cgattccctt
      601 gatcagtcgc tcgataccgg tgccgatcag gtccaacgcg tggtgtctcg gacgaatacg
      661 aaccggcccg acttgaacgc cgggcaacag tcgccgaaca gcgtcgggat cgagctggcc
      721 tggatgattc tcgagccagg ccccgatcgc gcctcttcgg cgggtatcga tgatctcggc
      781 tccacaacca gacgcaagct ttcggagtac ccggatgtag ccctgcgtga agtgatcgtc
      841 ccagctgtcc gaagtggcca gaaagaaggc gaccggtttg gcgtactcgg cccgaaatcc
      901 ctcggagtgg acctcaaccc ggccccacgc ttcgaccagt ccgacgacgg caccgtcgcg
      961 gtggccggaa tcctcgacaa gatgctggcc catgccgctc caggggtggt acgcatagag
     1021 cccgcactcg cagtcggcgt caggcgcttc atggcttgag gtcccgtttc cgcagctggc
     1081 tttggttggc gtcccgtcca ccgaccagct ttcaaccctg gtcagcgggc gaaggaaact
     1141 catgaacgag tcggtgcccc actctcgcac cccgatgatc gacccggcaa ggtacggaac
     1201 ctcagcttca taagggtcgg ctcttcgacc ggatctccct tgcgaccgga acttcatgac
     1261 cgtcgcggcg aacggcgtcg gcgcgggatg agccgggcga gtgtttccct tcggagggcg
     1321 cgtgggtcgt cgtgaatcca tgcgccgagc ttgtcgccct tgttacagtc gatcacctcg
     1381 gctccgcagc gttcggccag ttcaatcagt cggtattcgt aggcgcaccg tcgcttccgg
     1441 gttccgtacc accgcggatc ctcgtcctct ttcggcttcg gccaggtgaa gttgatgaag
     1501 gcgaccgggc gggcgaactc ggcccggaac ccttcgaggt gcagttcgat ccgaccccac
     1561 gcttcgacga agccgaccac cgttcgggga tcgggccggt gcagccggcg aagtatctgg
     1621 ggcggaaacc ggtaggcgtg atgcgcatag aggccgcagg tgcattcctt gccgggaacc
     1681 cggtggtatc ggttgtcgtg acgcacacag accgcggcgg tcgccccgcc atgggtctgc
     1741 catcgcgctc ccgtcgacga ggaccgaagc gaacaaagcc ccatgtccgt acgccagatg
     1801 cgaacaccca agatcgttcc cgcgatcaga ggggggttcg atattcggtt accttcaggc
     1861 accggcgggc accttggccg gccgcttctc cggtttgggt tcgggccgcg ggcgggccgg
     1921 acgctccggg atccgctttg gctcggcagg tgccggggtc tggtccggct gctcgagtgg
     1981 ctcgacgatg atcgtgcggg tcggtccgtc gggaaagccg gcccgg
//
LOCUS       DAOQMF010000354         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_37974_length_2022_cov_3.085918, whole genome shotgun sequence.
ACCESSION   DAOQMF010000354 DAOQMF010000000
VERSION     DAOQMF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37974_length_2022_cov_3.085918"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLV77_06510"
     CDS             <1..285
                     /locus_tag="PLV77_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HNC15534.1"
                     /translation="HPHTNMAKAALNMLTRTSAQEMLEADGILMTAVDTGWITDERPH
                     PMKKRLADDGFHAPLDLVDGAARVYDPIIRGEEGEDIHGVFLKDYKPSPW"
     gene            340..831
                     /locus_tag="PLV77_06515"
     CDS             340..831
                     /locus_tag="PLV77_06515"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC15535.1"
                     /translation="MSPEPSGEVERTAGGRDLILRRDLPLEPDQAWAWITESGKTGQW
                     FGTWTGAGEAGGEIEVTMVAEEGNPKSAMKINACEPGRSYEVTGSDESGGWNLELKLE
                     EGEGGTRLTFIHHLGEEDKVEMIGPGWEFYLDHLLAAIEGNEKKDFSEYWPAMGPYYA
                     ELG"
     gene            complement(833..906)
                     /locus_tag="PLV77_06520"
     tRNA            complement(833..906)
                     /locus_tag="PLV77_06520"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(870..872),aa:Asp,seq:gtc)
     gene            1075..1923
                     /locus_tag="PLV77_06525"
     CDS             1075..1923
                     /locus_tag="PLV77_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNC15536.1"
                     /translation="MRLVTYASERGPRAGVQTDQGVIDAWDLVDGEGASLRELIASGR
                     LDEVRTALDGGGGEPVMLSPGGILAPVPDPDKIICVGLNYRSHAEETGMELPKAPTIF
                     GKYRNALVPDGAKVTLPAASSKCDYEAEVAFVIGRRAREVSVEDALDHIAGYTLLNDL
                     SARDLQGLTMQWMAGKVFDGSAPCGPALVTPDEAGPHDGIGIRMLVNGEERQSSNTSD
                     LIFSVPQLVSTLSSWMTLEPGDIVATGTPSGVGLAMDPRTWLEDGDELVVSSPQLGEL
                     HTTIAR"
BASE COUNT          382 a          652 c          683 g          305 t
ORIGIN      
        1 catccccaca ccaacatggc caaggcggcg ctcaacatgc tgacccggac cagcgcccag
       61 gagatgctcg aggccgacgg catcctgatg accgcggtcg acacgggctg gatcaccgac
      121 gaacgccccc acccaatgaa gaagcgcctg gccgatgacg gcttccacgc cccgctcgac
      181 ctagtcgacg gcgccgcccg ggtctacgac ccgatcatcc gcggcgaaga aggcgaagac
      241 atccacggag tcttcctcaa ggactacaag ccttcgccct ggtgacctcc ggttgcgggc
      301 gaggtggaca tcgctgccct gacttgagaa cctgcggtca tgagccccga accttccgga
      361 gaagtcgaac gaaccgcggg cggccgcgac ctgatcctgc ggcgggacct gccgctcgaa
      421 cctgatcagg cctgggcctg gatcaccgag tcggggaaga ccggccagtg gttcggtacc
      481 tggaccggcg cgggggaagc cggcggtgaa atcgaggtga ccatggtcgc cgaggagggc
      541 aacccgaagt cggcgatgaa gatcaacgcc tgcgagccgg gccgctccta cgaggtgacc
      601 ggctcggatg agtccggcgg ctggaacctg gaactgaagc tggaggaagg ggaaggcggg
      661 accaggctga ccttcatcca ccacctcggt gaggaagaca aagtcgagat gatcgggccg
      721 ggctgggagt tctatctcga ccacctgctg gcggcaatcg agggaaacga gaagaaggac
      781 ttcagcgagt actggcccgc tatggggccg tactacgcgg agcttggatg aacgggagcg
      841 acgggacttg aacccgcggc ctctggcttg acaggccagc gctctaacca actgagctac
      901 gcccccgtga ggcgatcggt cgaaaccgac cgggcgggca ttatcgcagt ctcgaacgaa
      961 cggcgacatc tgacccttcc cgattcccgt tccgggggcg tttacccacc gtttgtgtgg
     1021 tcaatcgccc ccggttgccg atcgtttccg gcgttcgggt tctagactcg cgccatgaga
     1081 ctggtcacgt atgcatcgga gcggggcccg cgggctggag tccagaccga ccaaggcgtg
     1141 atcgacgcct gggacctggt tgacggcgag ggcgcaagcc tcagggaact gattgcctcc
     1201 ggccgcctcg acgaggtccg gaccgcgctg gacgggggcg gtggcgaacc cgtgatgctg
     1261 agcccgggcg gaatactcgc gccggttccc gatccggaca agatcatctg cgtcgggctg
     1321 aactaccgct cgcacgccga ggagaccggc atggaactgc cgaaggcccc gaccatcttc
     1381 ggcaagtacc gcaatgccct ggtcccggac ggagcgaagg tgaccctgcc agccgcgagc
     1441 agcaagtgtg attacgaggc agaggtcgcc tttgtgatcg gccggcgggc aagagaggtc
     1501 agcgtcgaag atgcgctcga ccatatcgcc ggctacaccc ttctcaacga cctttcggcc
     1561 cgcgacctgc agggcctgac catgcagtgg atggccggca aggtcttcga cggctcggct
     1621 ccctgcggcc ccgccctggt cactccggac gaggccgggc cgcatgacgg gatcggcatc
     1681 agaatgctgg tcaacggcga ggagcgccag agctcgaaca cctccgacct gatcttcagc
     1741 gtcccgcaac tggtctcgac cctttcgagc tggatgaccc tggagccggg cgacatcgtc
     1801 gccaccggaa ccccttccgg ggttggcctg gcgatggacc cgcgaacctg gcttgaagac
     1861 ggagacgaac tggtcgtcag ctcgccacag ctgggcgagc ttcacaccac gatcgcccgc
     1921 tagcaggctc aggggtgctt ggcgccggcg atttcgtttc ggaccggggg cagatcggcg
     1981 gagagcggtg cgctgagcgg tgccgatgaa gcggacgccc cg
//
LOCUS       DAOQMF010000355         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38005_length_2021_cov_3.762970, whole genome shotgun sequence.
ACCESSION   DAOQMF010000355 DAOQMF010000000
VERSION     DAOQMF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38005_length_2021_cov_3.762970"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /gene="ftsZ"
                     /locus_tag="PLV77_06530"
     CDS             complement(<1..1272)
                     /gene="ftsZ"
                     /locus_tag="PLV77_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNC15537.1"
                     /translation="MREGPLADLFRSTSDEPSQDPPEFEPRTADDTGGPQGDPVEPAA
                     AASTPAPEPEVHVEPDPDAAGRFGRRDPLEGRQEEPAPATPAEPAPRAEDPGPLSAPD
                     PAAETSNVPDAQDRLKRIFSEAPQDRDDGPRYGRQEPSSTPGAGVPHNPVIRVIGVGG
                     GGVNAINRMIEAQIPGVEFMAVNTDLQSLQLCMADLTVHIGGDETRGLGAGADPSLGF
                     RAAFDEQDRIKRLLKGSDMVFIAAGAGGGTGTGAAPVIARLARDIGALTVGIVTKPFT
                     FEGSRRQKQADQGIEALAGEVDTLIVIPNDRLLEVLDQSTPMVDAFQVADDVLRQGVQ
                     GISDLVTLPSVINLDFADVRSIMSDAGRALLGIGMGSGPDRALIAAEKATSSPLLETP
                     MDGAKSILLSVTGGPDLSLIEVSEAARAVGEA"
     gene            complement(1283..>2021)
                     /locus_tag="PLV77_06535"
     CDS             complement(1283..>2021)
                     /locus_tag="PLV77_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC15538.1"
                     /translation="ANQKGGVAKTTTTLNLAVALSELGNRVLCIDLDPQGNLTMSQGI
                     DPDKCEKSMYDVLVDDLPISEVIAHREIDIAVASIDLAGAEIAMSTKIGRERSLEKAL
                     KEVHSDYDYICIDTPPSLGLLTINALTASSKVIVPVQCEYLSMRGLVQLQSTLKMIQE
                     NLNPNVHIEGILPTMFDSRTLHAREAVFILEENFGDLVFKSRIRKAVKFAEAPVRGAS
                     VLKYDPESRAASYYRNLAKEVLSDGSS"
BASE COUNT          324 a          636 c          700 g          361 t
ORIGIN      
        1 tgcctcaccg accgcacggg ccgcctcgga aacctcgatc agcgaaaggt cggggccgcc
       61 ggtgaccgag agcaggattg acttggcgcc gtccatcggg gtttccagca gaggtgagct
      121 ggttgccttc tcggcggcga tcagcgcccg gtcggggccg ctgcccatgc cgatcccgag
      181 cagcgcccgg ccggcgtcgg acatgatcga ccgcacgtcg gcgaagtcga ggttgatcac
      241 cgagggcagc gtgaccaggt cggaaatgcc ctgaacaccc tgccgcagca cgtcgtcggc
      301 aacctggaag gcgtcgacca tcggggtcga ctggtcgagc acctcgagca gacggtcgtt
      361 cggaatcacg atcagcgtgt cgacttcacc ggcgagggct tcgatcccct ggtcggcctg
      421 cttctggcga cggctgccct cgaaggtaaa cggcttggtc acgatcccga ccgtaagcgc
      481 accgatgtcg cgggccagcc gggcaatgac cggcgcggca ccggttccgg tgccaccacc
      541 ggcaccggcc gcgatgaaga ccatgtcgga gcccttgagc agccgcttga tccggtcctg
      601 ctcgtcaaag gcggcccgga aaccgagcga agggtccgct ccggcaccga gtccgcgggt
      661 ctcgtcgcca ccgatgtgga cggtcaggtc cgccatgcag agctggagcg actgaaggtc
      721 ggtgttgacc gccatgaact cgacacccgg gatctgggcc tcgatcatgc ggttgatcgc
      781 gttgacgcca cccccgccga ctccgatcac ccggatcacc gggttgtgtg gaactccggc
      841 tccgggagtc gaggaaggtt cctgacggcc gtagcggggt ccgtcgtcgc gatcctgcgg
      901 agcctcggag aaaatccgct tcagtcggtc ctgggcgtcg ggcacattgg aagtttcagc
      961 agccgggtcg ggggccgaaa gcgggcccgg atcctccgcc cgcggggcag gctcggccgg
     1021 ggtggcggga gccggttctt cctgtcggcc ctccagcggg tcgcggcgac cgaagcggcc
     1081 ggcggcgtcg ggatcgggct cgacgtgaac ctcgggctca ggtgcgggcg tcgaggctgc
     1141 ggctgcgggc tcaaccgggt cgccctgcgg tccgccggtg tcgtcggcgg tacgaggttc
     1201 gaattcgggt ggatcctggg agggttcgtc ggaggtagag cggaacaggt ccgccagcgg
     1261 accttcacgc atactcgctc gcttacgacg agccatcact caagacctcc ttcgccagat
     1321 tccggtagta cgacgccgca cgactctcgg gatcgtactt caggacacta gcgcctcgta
     1381 ccggcgcctc ggcgaacttc acggccttgc ggattcggga cttgaagacg aggtccccga
     1441 aattctcctc gaggatgaac acggcttccc gcgcgtgaag ggtgcgggag tcgaacatgg
     1501 tcggcaggat gccttcgatg tggacgttcg ggttcaggtt ctcctgaatc atcttcaggg
     1561 tgctctgaag ctggacgagg ccgcgcatcg agaggtactc gcactgaacc gggacgatca
     1621 ccttgctcga agccgtcagc gcgttgatgg tcaggagacc aaggctcggt ggggtatcga
     1681 tgcagatgta gtcgtagtcc gagtggactt ccttgagcgc cttctcgagc gaacgttccc
     1741 gcccgatctt ggtgctcatg gcgatctcgg cgccggccag gtcgatcgag gcgacagcga
     1801 tgtcgatctc ccggtgggcg atcacttcgg agatcggcag gtcgtcgacc agcacgtcgt
     1861 acatgctctt ttcgcatttg tccgggtcga ttccctggct catggtcagg ttgccctgcg
     1921 ggtcaaggtc gatgcagagg acccggttgc cgagctcgga gagggccacc gccaggttca
     1981 gggtcgtcgt cgtcttggcg acgccaccct tctggttggc g
//
LOCUS       DAOQMF010000356         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38060_length_2020_cov_2.291603, whole genome shotgun sequence.
ACCESSION   DAOQMF010000356 DAOQMF010000000
VERSION     DAOQMF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38060_length_2020_cov_2.291603"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLV77_06540"
     CDS             <1..501
                     /locus_tag="PLV77_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC15539.1"
                     /translation="ARALAPEPSLIVADEPVSALDVSIQGQIVNLMADLQRELELTYL
                     FVAHDLGVVRQVSDRIAVMYLGKIVEIGPAELVYSKPIHPYTASLLAAVPIPDPRTNA
                     QRERRPLEGDVPSPSDPPSGCRFHTRCPYATEVCEQQEPALIDYGQGHFAACHHPLGR
                     PDPAGE"
     gene            538..945
                     /locus_tag="PLV77_06545"
     CDS             538..945
                     /locus_tag="PLV77_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNC15540.1"
                     /translation="MAELEGRSLEIVKEGANYAVVAVPTDDGSVQSVIVWAHADDDGN
                     VILNSAEGRAWPQNLRKAGRATVTMMADGNPYEYVSIRGRLIGDTQEGADDDINMLAK
                     KYIDADEYPFRQPGEVRVTFTLEPEKVNYLKQG"
     gene            1082..>2020
                     /locus_tag="PLV77_06550"
     CDS             1082..>2020
                     /locus_tag="PLV77_06550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC15541.1"
                     /translation="MSVLAILAGAAFATLAMIGPAAAAGKSLDYVALGDSYSAASGVL
                     PLDPEAPVQCLRSTLNYPHVLAAKLGAELTDVTCGAAETRHFGESQYPGVAPQLDALS
                     KDTDLVTMTIGGNDSSVFIGSILSCGTLGVASLGQGSPCKDTFGDQFEDTIRNTTYPS
                     LVEALNAVHAKAPKARVAILGYPWIMPETDGCYPQMPVSKGDVPYVRSLQRTLNDAVG
                     RAARETGSVFVNLNGVSNGHDACQAPGIRWVEPALLGENPVIVHPNALGEAKMAEQAF
                     KVLSKSGTPAPAAPRTRISKVLISNGKRRATFRLEST"
BASE COUNT          358 a          686 c          641 g          335 t
ORIGIN      
        1 gcccgtgccc ttgcgcccga accgagcctg atcgtcgccg acgaaccggt ctcggcgctc
       61 gacgtttcga ttcaggggca gatcgtgaat ctgatggccg acctccagcg cgagctggag
      121 ctgacctacc tcttcgtggc gcatgacctg ggcgtggtcc ggcaggtgtc cgaccggatc
      181 gccgtgatgt atctgggcaa gatcgtcgag atcggcccgg ccgaactcgt ctactcgaaa
      241 ccgatccatc cctacacggc ctcgctgctg gcggccgtcc cgattcccga cccgagaacc
      301 aacgcacaga gggagcgccg gccgctcgag ggtgacgttc cgagcccctc ggacccgccc
      361 tcgggttgcc gtttccacac ccgctgcccc tacgcaaccg aagtctgcga gcaacaggag
      421 ccggccctga tcgactacgg ccagggtcat ttcgccgcct gccaccatcc cctcggtcga
      481 ccggacccgg ccggggagta gattcggtcc tgtcttcaaa caacccagga ggtcgaagtg
      541 gcagaactcg aaggtcgttc gctcgaaatc gtcaaggaag gtgcgaacta tgcggtcgtc
      601 gccgtgccga ccgatgacgg ctcggtgcag tcggtgatcg tctgggcgca cgccgacgac
      661 gacggcaacg tgatcctgaa cagtgccgag ggtcgggcct ggccgcagaa cctgcgcaag
      721 gcgggccggg cgaccgtgac gatgatggcc gacggcaacc cctacgagta cgtgtcgatc
      781 cgcggccggc tgatcggtga tacccaggaa ggcgccgacg acgacatcaa catgctggcc
      841 aagaagtaca tcgacgccga cgagtatccg ttccgccagc cgggcgaggt ccgggtcact
      901 ttcaccctcg aacccgagaa ggtcaactac ctcaagcagg gttagccgca gaacccggga
      961 gggttggcct ggccgcatct tcccggtttg gagttcgaat cccggggtag tcacagagcg
     1021 ctcaggaacg ctggtgttct cgactacccc gggagatttg atgaagcttc gcagtcgttg
     1081 tgtgtccgtc ctcgcaattc tggccggtgc ggcattcgcc acgctcgcca tgatcgggcc
     1141 ggcggccgcg gccggcaagt cactcgacta tgtggcactc ggcgactcct acagcgccgc
     1201 ttccggggtt ctgccgctcg accccgaagc cccggtccag tgcctgcgct cgactcttaa
     1261 ctatccgcat gttctggctg caaagctggg cgccgagctg accgacgtga cctgcggcgc
     1321 ggccgaaacc aggcacttcg gcgagtccca gtaccccggg gtggcgccac agctcgacgc
     1381 gctctcaaag gacaccgacc tcgtcacgat gaccatcggc ggcaatgaca gcagcgtctt
     1441 catcggctcg atcctgagct gtggcacgct cggggttgcc tcgctgggtc agggcagccc
     1501 ctgcaaggac accttcggcg accagttcga ggacacgatc cggaacacca cctacccgtc
     1561 actggtcgaa gccctgaacg ctgttcacgc gaaggcgccg aaggcccggg tcgcaatcct
     1621 cggctacccc tggatcatgc ccgagaccga tggctgctac ccgcagatgc cggtctcgaa
     1681 gggtgacgtt ccctatgtcc gctcgctgca gcggacgctg aacgatgcgg ttggacgcgc
     1741 ggcccgtgaa accggttcgg tcttcgtcaa cctgaacggc gtgtcaaacg ggcacgacgc
     1801 ctgccaggcc ccgggaatcc gctgggtcga gccggcgctg cttggcgaga acccggtgat
     1861 cgtccatccg aacgccctcg gtgaagcgaa gatggccgag caggccttca aggtgctttc
     1921 gaagtcgggc acgccggcgc cggccgctcc ccgcacgcgg atcagcaagg tgctgatcag
     1981 caacggcaag cggcgggcca ccttccggct tgagtcgacc
//
LOCUS       DAOQMF010000357         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38233_length_2015_cov_2.845408, whole genome shotgun sequence.
ACCESSION   DAOQMF010000357 DAOQMF010000000
VERSION     DAOQMF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38233_length_2015_cov_2.845408"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2015)
                     /locus_tag="PLV77_06555"
     CDS             complement(<1..>2015)
                     /locus_tag="PLV77_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC15542.1"
                     /translation="LEDQPGLVDSALQVIDEFRAALISPKEFDDPAISDLAGPAAGVF
                     RLYLDGLHEAGLTDLPERSVLATTHSLDSWEGRPVFVAGFDDLTRQQLDLLRRLSGKT
                     DVTIAITHEQGNEAMAITESLLGRLREIGGTVERETERPAEQADHEGLLVELERSFLD
                     PGKAGTIKPGAALKLIEASGRRGEAEAIGAEIAGLVSNGTDPGEIAIAIDAPATNGTA
                     LLEVLTEYEIPAMLEAETTAPESAIGQSAINFLLATARNGPADRLFAWLRGPLGLDPD
                     LIDQLEFRAVRSGTESAREVIAAMNPAQRRGLPGLAEGENGDPSEAVRSVVRHGARVL
                     LAERQDSIPASSIATETQMASAISRACDELEGIHGDHLSARILIDALASGSVKTWAVP
                     TAHTVRIGSPYAMRAKRVEYLFIASLQEKDLGGDQSSPLISNEARSALGMPELADQEE
                     QETYLFYSCLAVPTRGLWLSHRVADVHGKSEFPSAMIGEVTRLFEGEGAGLDLIRRSS
                     SDIIFPVADSPSRHEWAISIAAAGEAVDPLGSDAAKEVVLEVGRSREVEASTSTLGDI
                     DSPAAAAALAERNVFSATALEAFIECPYRWFFERAMNPVRFGPEPEPLAKGNLVHEVL
                     AALYSSRRATIPNGDDIEDWIAAARTLVDELSGQCDLGGDSARHRIQ"
BASE COUNT          328 a          669 c          659 g          359 t
ORIGIN      
        1 ctgaatccgg tggcgggccg aatctccgcc caggtcgcat tgcccggaca attcgtccac
       61 gagcgttcgc gccgcggcga tccagtcctc gatgtcgtcg ccattcggga tcgttgcccg
      121 acgcgaggag tagagagcgg ccagaacctc gtgcacgagg ttgcccttcg ccaggggttc
      181 gggctccggt ccgaatcgca ccggattcat cgcccgctcg aagaaccatc ggtagggaca
      241 ttcgatgaac gcctcaaggg cggtcgccga gaaaacgttc cgttcggcga gcgcggcagc
      301 agccgcaggg gaatcgatgt cacccagggt cgaggtggat gcttcgactt cacgcgaccg
      361 tccgacctcg aggacgactt ccttcgccgc gtccgagccg agcgggtcca ctgcttcgcc
      421 ggcggcggcg atcgagatcg cccactcgtg ccggctgggt gaatcggcga cgggaaagat
      481 gatgtccgag ctcgatcgcc ggatcaggtc gaggccggcc ccttcgcctt cgaagagacg
      541 cgtcacctcc ccgatcatcg ccgagggaaa ttcagacttg ccgtggacgt cggccacccg
      601 atgggagagc cagaggccgc gagtcgggac cgcgaggcag gagtagaaga gataggtctc
      661 ctgttcttcc tgatcggcca gctccggcat gcccagcgcc gagcgggctt cgttcgagat
      721 gagggggctg ctctgatcgc caccgagatc cttctcctga agcgaagcga tgaacaggta
      781 ttcgaccctt tttgcccgca ttgcgtacgg actgccgatc ctcaccgtgt gtgcggtcgg
      841 cacggcccag gtcttgaccg acccgctggc cagggcatca atcaggatcc gggccgagag
      901 atggtcgccg tgaatccctt ccagctcgtc gcaggcacgg ctgatcgcac tcgccatctg
      961 ggtctcggtg gcgatcgaag aggcagggat cgaatcctgc cgctcggcca gcaaaaccct
     1021 tgctccgtgt cgcacaaccg atctgaccgc ctcggacggg tctccgttct cgccctcggc
     1081 gaggccaggc agcccgcggc gctgagccgg gttcatcgct gcgatcactt cccgcgcgct
     1141 ctcggtgccc gagcgaaccg ccctgaactc gagctggtca atcaggtcag ggtcgagacc
     1201 gagcgggcct ctcagccatg cgaacagccg gtcggcgggt ccgttccggg cggtggccag
     1261 gaggaagttg atcgcggact ggccgatcgc cgattccggt gccgtcgttt cggcttcgag
     1321 catcgccggg atctcgtact cggtgagcac ctccagaagc gcggtcccgt tggtcgccgg
     1381 agcgtcgatg gcgatcgcga tctctccggg atcggtcccg ttgctgacca ggcccgcgat
     1441 ctccgcgccg atcgcttcgg cctccccgcg gcgacccgag gcctcgatca gtttgagggc
     1501 ggcaccgggc ttgatcgttc ccgctttccc ggggtcgagg aagcttcgct cgagctcgac
     1561 cagcaggcct tcgtgatcgg cttgctccgc gggacgttcc gtttcccgtt ccaccgtccc
     1621 cccgatctcc ctgagtcgtc ccagcaggct ctcggtgatc gccatcgcct cgttgccctg
     1681 ctcgtgagtg atcgcgatcg tcacgtcggt ctttccggag aggcggcgga ggagatcgag
     1741 ctgctgccgg gtcaggtcat cgaagccggc gacgaatacg ggacgacctt cccacgaatc
     1801 gagtgaatga gtcgtagcca gcacggacct ctcgggcaga tcggtcagtc cggcctcgtg
     1861 cagtccgtcc aggtagaggc ggaagacccc cgcggcaggt ccggccagat cggaaatggc
     1921 aggatcatcg aactccttcg gggaaatcag ggcggcccgg aactcgtcga ttacctgaag
     1981 cgccgagtcg accagcccgg gctgatcttc cagcc
//
LOCUS       DAOQMF010000358         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38234_length_2015_cov_2.799490, whole genome shotgun sequence.
ACCESSION   DAOQMF010000358 DAOQMF010000000
VERSION     DAOQMF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38234_length_2015_cov_2.799490"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLV77_06560"
     CDS             <1..372
                     /locus_tag="PLV77_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15543.1"
                     /translation="NTDTLAPYARAAYTGLDDNRGKLHLIALPRGKSSAGYLEHGRIS
                     SSEGKRSWKLTVKGAGQVKLDLDASLKTLKHPFRPGSVKAGGKRLKKGKAWSYDRKTG
                     VLHVNTKLKAGQTLSVSAANR"
     gene            407..>2015
                     /locus_tag="PLV77_06565"
     CDS             407..>2015
                     /locus_tag="PLV77_06565"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNC15544.1"
                     /translation="MRRIRFAIAGAAMLCAAAILPAAASADCNLPTGNGGSNNTALSG
                     GTTQTFSGHFGPDLNGKFIQIPFDVSSGITGMKIRYCYAKATGGEADDSPTLDLGVYD
                     AKPAEATTWTQAERRGWSGSALRTIGIGVNGYSDPATYEGGRKNYVPGRTTRAFKPGP
                     IEAGQWAVEFGAGWIDPDGAGVDWKLEVITSTAPEWSNDAFTADPYAPYSANPNPGWY
                     TGDLHAHGEMEPGNATMKQTMDLGFGALPAGNGLDFMTVVDHNNSNSLGVLGEDQYSY
                     PGKLVIPGIEVTTYNGHWNAIGSSNFADFRFSDVLRWDDAGDDNIQTDGELSTVRPAI
                     QPASQMQPILDGGGYTQVNHPETLKAAPAQCRGCAWTYSDAQTDWSKVTALEIQNGPA
                     GIPAGSPTAMNPFSVDAIKTYERLLADGFHIAAVGSSDDHQGGEATGAFDSPVGRGAT
                     VVHADQLSTQGVIDAVKAGHTYVKPFGADAPDVELSASTPQGGKAIPGDSVTGSSLAI
                     KIAVEGAASAVRPGPYTLKLLQDGIEVD"
BASE COUNT          399 a          695 c          637 g          284 t
ORIGIN      
        1 aacactgaca ccctggcgcc ctatgccagg gcggcctaca cgggcctcga cgacaatcgc
       61 ggcaagctcc acctgatcgc cctgccgcgc ggcaagagtt cggccggcta cctcgagcac
      121 ggccggatca gctcgagcga gggcaagaga agctggaagc tcacggtgaa gggcgccggc
      181 caggtcaaac tcgacctcga cgcttcgctg aagaccctga agcacccctt ccggccgggt
      241 tcggtcaagg ccggcggaaa acgcctgaag aagggcaagg cgtggagtta cgaccggaag
      301 accggcgtgc tccacgtgaa cacgaaactg aaagccggcc agacgctcag cgtctcggcc
      361 gcgaaccgat gaccaccgcc gaccccgaca ggaataacct gagccaatga ggagaatccg
      421 attcgcaatt gccggtgccg ccatgctgtg cgcggccgcc atcctgcccg ccgccgcttc
      481 ggctgactgc aacctgccga ccggcaacgg gggcagcaac aacaccgccc tgtccggcgg
      541 caccacccag actttttccg gccacttcgg gccggacctg aacggcaagt tcatccagat
      601 ccccttcgat gtgagcagcg gaatcaccgg catgaagatc cgctactgct acgcgaaagc
      661 caccggcggc gaggcggatg actccccgac cctcgacctc ggcgtctacg acgcgaagcc
      721 ggccgaagcg accacctgga cccaggccga gcggcgcggc tggagcggca gcgcactgcg
      781 gactatcggg atcggcgtga acggttactc ggatccggct acctatgaag gcggccgcaa
      841 gaactacgtg cctggccgca ccacccgcgc gttcaagccc ggcccgatcg aagcggggca
      901 gtgggcggtc gagttcggtg ccggctggat cgacccggat ggtgccgggg ttgactggaa
      961 gttggaagta atcaccagca ccgcaccgga gtggagcaac gacgccttca ccgccgatcc
     1021 gtacgcgccc tacagcgcga acccgaatcc cggctggtac accggtgacc tgcacgctca
     1081 cggtgaaatg gaacccggta acgcgaccat gaagcagacg atggatcttg gtttcggagc
     1141 gctgcccgcc ggcaacggac tcgacttcat gaccgtggtc gaccacaaca acagcaactc
     1201 gctcggggtg ctcggtgagg accagtacag ctacccgggc aagctggtca ttccgggcat
     1261 cgaggtcacc acgtacaacg gccactggaa cgcgatcggt tcgagcaact tcgccgactt
     1321 ccggttcagt gacgtactgc gttgggacga cgccggcgac gacaacatcc agaccgacgg
     1381 cgaactcagc acggttcgcc ccgcgatcca gcccgcctcg cagatgcagc cgatcctcga
     1441 cggcggcggc tacactcagg tcaatcaccc ggagaccctg aaggctgcgc cggcccagtg
     1501 ccgcggctgc gcctggacct actcggacgc gcagaccgac tggagcaagg tcaccgccct
     1561 ggaaatccag aacggcccgg ccgggattcc ggccggttcg ccgaccgcaa tgaacccttt
     1621 ctcggtcgac gcgattaaga cctacgagcg gctgctcgcg gatgggtttc acatcgccgc
     1681 ggtcggctcc agtgacgacc accagggtgg cgaagcgacg ggagccttcg attccccggt
     1741 cggccgggga gccaccgtgg tccacgccga tcagctctcg acccagggcg tgatcgacgc
     1801 ggtcaaggcc gggcacacct acgtgaagcc cttcggcgcc gatgcccccg atgtcgagct
     1861 ctccgcctcg accccgcagg gtggcaaggc gattcccggt gattcggtta ccggatcgag
     1921 cctcgcaatc aagatcgccg tcgagggcgc ggccagcgcg gttcgcccag gtccctacac
     1981 gctgaagctg ctgcaggacg gcatcgaggt tgatt
//
LOCUS       DAOQMF010000359         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38235_length_2015_cov_2.781633, whole genome shotgun sequence.
ACCESSION   DAOQMF010000359 DAOQMF010000000
VERSION     DAOQMF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38235_length_2015_cov_2.781633"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PLV77_06570"
     CDS             <1..1352
                     /locus_tag="PLV77_06570"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNC15545.1"
                     /translation="LYATMAGVVAVAGAALLRRRRFERLLSRSEDFVGPEAGRGLTPK
                     QRARELERLASALEARDPRTHGHSRRVARHATSIARGLRLPDAEVARIRTAALLHDVG
                     KIEVPWEILEKPGALTDEEFAQIKKHSAAGARLVEGMGDPELVAIVRHHHERIDGGGY
                     PDRIAGDEIPIGARIIAVADTFDALTSARSYRQPRSHEEALGVLREEAGAQLDAGAVQ
                     AFDGRYSSRRPVALVATVLGLGRQAGQSMISFGTGASQVAAVGAAAAVIGVAPAARVD
                     QQQEPAKQNPIVRQSDSTSARSVADSGSATAGSQSGTGEQGRKSASGGRNARNGNGGK
                     GSTDNTFSTSGDGGGTSSGSNGGSGSGGGSSGGSGGGSGGGSSSGGSSGSGTTAVNPV
                     QTVTENVQKQVQQTTQQVTDSIPSLPGNDPVSQGVNNTVDEVKDTVNSTVGKLTGKP"
     gene            complement(1362..>2015)
                     /locus_tag="PLV77_06575"
     CDS             complement(1362..>2015)
                     /locus_tag="PLV77_06575"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNC15546.1"
                     /translation="IASVLVLDRENAEVVTLAGSECGFGYRSSRFKGDSRFLVLAVEF
                     ILSPSEMSAPVEYAQLAGALEVEVGERVPLPVARETVLELRRSKSMVVDPADPDSVSA
                     GSFFTNPLLDLASMADLTARAEELFGADDLPPGYPAPEGLAKTSAAWLIERSGFTRDF
                     GDGPIGLSRNHTLAIVNRGGGTTAELVAFAHGIADRVEEIFGVALVPEPVFVGHAWR"
BASE COUNT          336 a          698 c          696 g          285 t
ORIGIN      
        1 cgctctatgc gacgatggcc ggcgtcgtgg cggtcgccgg tgcggcgttg ctccgccgcc
       61 gccgcttcga aaggctgctt tcccgctcgg aggacttcgt cggcccggag gcgggccgcg
      121 ggctgactcc gaagcagcgg gcccgtgagc ttgaacgcct ggccagcgcc ctcgaggctc
      181 gtgatccacg cacccacggc cactcgcgcc gggtggcgag gcatgccacc tcgatcgccc
      241 ggggcctcag gttgcccgat gccgaagtcg cccggattcg caccgcggcg ctgcttcatg
      301 acgtcggcaa gatcgaggtg ccctgggaaa tcctcgaaaa gcccggcgcc ctgaccgacg
      361 aggaattcgc ccagatcaag aagcattccg ccgccggggc ccgcctggtc gaagggatgg
      421 gtgacccgga gctcgtcgcg atcgtccggc accaccacga gcggattgac ggcggcgggt
      481 acccggaccg catcgccggt gacgaaatcc cgatcggggc ccggatcatc gccgttgccg
      541 acaccttcga tgccctcacc tcggcccgtt cctaccgcca gcctcgttcg catgaggagg
      601 cgctcggggt gctccgggaa gaggccgggg cgcagcttga tgccggcgcg gtccaggctt
      661 tcgacggccg ctattccagc cgccggccgg tcgccttggt tgccacggtc ctcgggctcg
      721 gcaggcaggc cggccagtcg atgatcagct tcggaaccgg tgcttcgcag gtcgcggcgg
      781 tcggggcggc cgcggcagtg atcggtgtcg cccccgcggc cagggtggac cagcagcagg
      841 aaccggcgaa gcagaacccg atcgtgcggc agtcggattc cacctcggcc cggtcggtgg
      901 ctgattccgg ctccgcaacg gccggcagcc agtccgggac cggagagcag ggccgcaagt
      961 cggcatccgg cggccggaac gcccgaaacg gcaacggcgg caaaggatca accgataaca
     1021 ccttcagcac ttccggggac ggcggcggaa ccagctccgg atcaaacggc ggctccgggt
     1081 ccggcggagg atcctccggt ggctcaggtg gcggcagcgg cggcggctct tccagcggcg
     1141 gctcgtcagg cagcggcacg accgcggtca accctgtcca gactgtgacc gagaacgtgc
     1201 agaagcaggt tcagcagacg acccagcagg tgaccgattc gatcccctcc ctgcccggca
     1261 acgacccggt ttcccagggt gtcaacaaca cggttgacga ggtcaaggac acggtcaaca
     1321 gcacggtcgg caagctcacc ggcaagccct gaccatttcc cctagcgcca ggcgtggccg
     1381 acgaagaccg gctccgggac cagcgccacc ccgaagattt cttccacccg gtcggcgatg
     1441 ccgtgggcga aggcgaccag ttccgcggtc gtgcccccgc cgcgattgac gatcgcaagg
     1501 gtgtggttcc ttgacagccc gatcggtcca tccccgaaat cgcgggtgaa tccggaccgc
     1561 tcgataagcc aggcggccga agtcttggcg aggccttccg gcgccgggta gcccggcggc
     1621 aggtcatcgg ctccgaacag ttcctccgcc cgggcggtca ggtccgccat gctggccaga
     1681 tcgagcaggg ggttggtgaa aaacgagccg gcgctgaccg aatccggatc ggccgggtcg
     1741 accaccatgc tcttcgagcg cctcagctcg agcacggttt cgcgggcgac cggcagggga
     1801 acccgttcgc cgacctccac ttcgagcgcc ccggcgagct gggcgtactc gaccggcgcc
     1861 gacatctcgg aagggctgag gatgaactcg accgccagca cgagaaagcg cgaatcaccc
     1921 ttgaagcggc tcgagcggta gccgaagccg cattcgcttc cggcgagggt gacgacttcg
     1981 gcgttctcgc gatcgagcac caggacggaa gcgat
//
LOCUS       DAOQMF010000360         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38259_length_2014_cov_4.231240, whole genome shotgun sequence.
ACCESSION   DAOQMF010000360 DAOQMF010000000
VERSION     DAOQMF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38259_length_2014_cov_4.231240"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PLV77_06580"
     CDS             complement(<1..1430)
                     /locus_tag="PLV77_06580"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNC15547.1"
                     /translation="MLSSIIWTPLLFGLIGLLVPRRFAAWVAALGAVITLGLSIGVLA
                     GFDPDGGMQFVTDVSWIPGLGVDYSLGIDGVSIFLVMLTAIAWIPAIGFSALRGTERP
                     ALFYLMLLAGETSTLGAFLAQDLLLFVLFFDLMIVPFYFLFGIWGRDRGGESDPEGSP
                     GNGPVTVQSATLKMIVFTLIGSLLMLVGAIAAAVIAADGGELTFSIQTIVARGLPEGS
                     QNWIFWFFAAAFLVKMPAFPLHGWMPDSYRAAPLPALAVFSAVLSKVGAYGFLRVVLP
                     MMPDAADLFQTTMLVLAIASILYGSAMAFTKTDLRLVLGFSSVAQLGFITAGIFAFNE
                     SGAEGAVLQMVNHGLVVVPAFLIVAMISERTGSEELGPMGGLAKKAPVFAVIFLIVSM
                     ATLAIPASANFIGEFFILNGLFQVDAAWAIVASVGVALAAFYALRMYQLTMHNPLPDG
                     SDSKEISLRDALVVIPMVAIVVVLAF"
     gene            complement(1430..>2014)
                     /locus_tag="PLV77_06585"
     CDS             complement(1430..>2014)
                     /locus_tag="PLV77_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNC15548.1"
                     /translation="SEVGYPGEEHHIAEQAGGMKFAMAVLGVLALIAGVLQIPGLDDV
                     VTKFLEPTFENSVFSHMHPTTSESWIGLGEGALISLAGIATAWLLYIKRPDLPVRFRE
                     RFRWIHTLTVNKWYGDEIIDFLIVNPVKAIGRFCNRAFERFVIDGATTGVAGLTRSAG
                     AAVRDMQSGLLRGYAVLMLFGILAIVIYFLIRSI"
BASE COUNT          427 a          621 c          698 g          268 t
ORIGIN      
        1 cagaaggcca gcacgaccac gatggcgacc atcgggatca ccaccagggc atcccgcagc
       61 gagatctcct tcgagtcgga gccgtcgggc agcgggttgt gcatggtcag ctggtacatc
      121 cggagcgcat agaaggcggc cagggcgaca ccgaccgagg cgacgatggc ccaggccgcg
      181 tcaacctgga agagcccgtt gaggatgaag aactcgccga tgaagttggc cgaggcgggg
      241 atcgccaggg ttgccatcga gacgatcagg aagatcaccg cgaagaccgg cgccttcttg
      301 gccagtccgc ccatcgggcc aagctcctcg gagccggtcc gttcactgat catcgccacg
      361 atcaggaagg cggggaccac gaccagtccg tggttgacca tctgcagcac cgcgccctcc
      421 gcaccggatt cattgaaggc gaatatgccg gccgtgatga atcccagctg ggcgaccgaa
      481 gagaaaccga gcacgagccg caggtcggtc ttggtgaagg ccatcgccga gccgtagagg
      541 atggaggcga tcgcgaggac cagcatcgtc gtctggaaga ggtcggcagc gtcgggcatc
      601 atcggcagca cgaccctgag gaagccgtaa gcgcccacct tcgagagcac ggcggagaag
      661 accgccaggg ccggcagcgg cgccgcccgg taggagtcgg gcatccagcc gtggagcggg
      721 aaggccggca tcttgaccag gaaggcggca gcgaagaacc agaagatcca gttctgggaa
      781 ccctcgggca ggccgcgggc gacgatcgtc tggatcgaga aagtcagctc tcccccgtcg
      841 gccgcgatca ccgcggcggc aatcgctccg accagcatca gcagcgagcc gatcagggtg
      901 aagacgatca tcttcagggt cgctgactgc accgtgaccg gtccgtttcc gggacttcct
      961 tccgggtcgc tttcgccacc ccggtcgcgg ccccagatcc cgaagaggaa gtagaagggc
     1021 acgatcatca ggtcgaagaa gaggacgaag agcagcaggt cctgggcgag gaaggcgccg
     1081 agggtcgagg tttcgccggc cagcagcatc aggtagaaga gggccggccg ttcggtcccc
     1141 cgcagggccg agaaaccgat cgcggggatc caggcgatcg cggtcagcat gaccaggaag
     1201 atcgagaccc cgtcgatccc gaggctgtaa tcgacgccga ggccggggat ccaggagacg
     1261 tcggtgacga actgcatgcc gccatcgggg tcgaagccgg ccagcactcc gatcgagagg
     1321 ccgagcgtga tcaccgcgcc gagcgccgcg acccaggcgg cgaaacgccg ggggaccagc
     1381 agcccgatca ggccgaagag aagcggagtc cagatgatcg aactcagcat cagatgctcc
     1441 ggatcaggaa gtagatgacg atcgcgagga tgccgaagag catcagcacg gcgtagccgc
     1501 gaagcaggcc ggactgcatg tcacgaaccg cggcgccggc cgaacgggta agtccggcga
     1561 ccccggtggt ggcaccgtcg atcacgaaac gctcaaaggc gcggttgcag aagcgaccga
     1621 tcgccttgac cggattcacg atcaggaaat cgatgatctc gtcgccgtac cacttgttga
     1681 ccgtgagggt gtggatccac ctgaagcgct cgcggaaccg gaccggcagg tcgggccgct
     1741 tgatgtagag cagccaggcg gtggcgatgc cggccagcga aatcagggca ccctcgccga
     1801 ggccgatcca ggattcactg gtggtcggat gcatgtggga gaagaccgag ttctcgaagg
     1861 tcggttcgag gaacttggtg accacgtcgt cgagcccggg gatctgaagc acgccggcga
     1921 tcagggccag cacgccgagc accgccatcg cgaacttcat cccgccggcc tgctcggcga
     1981 tgtgatgctc ctcgccgggg tagccgacct cgct
//
LOCUS       DAOQMF010000361         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38304_length_2013_cov_3.426966, whole genome shotgun sequence.
ACCESSION   DAOQMF010000361 DAOQMF010000000
VERSION     DAOQMF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38304_length_2013_cov_3.426966"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLV77_06590"
     CDS             <1..798
                     /locus_tag="PLV77_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC15549.1"
                     /translation="PGTGGAPEVLGSVQYFFRLLGAAAARALELEYGLLVADPRKGAG
                     QTALDGAIVVDPAIDDPVVAACDTAGLPVVTIGRRLASDESGKAGTWVVDNDFLAATG
                     SVLDHLAGQGARSIALMASEPIDSFQQDSIDAYRQWCAGHGVEARVVVSQSPDPKDAT
                     VAAGRLFDQGQMPDGVYATIDTLARALLDRAASERVAVPGELRIATCSNGELARTSQP
                     QLTTLDEHPATLGEEAVELLVRAIEDPESAPSFVKVDTELLVRESTA"
     gene            complement(827..1975)
                     /locus_tag="PLV77_06595"
     CDS             complement(827..1975)
                     /locus_tag="PLV77_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC15550.1"
                     /translation="MNFDLTDEQRLLQNTVRDFARNEVVPVAEELDRTKAFPYEIVAK
                     LGDLGLMGIPFPEEYGGSGGDILSYALAVEELARIDSSVAITMAAHISLGTTPIYNWG
                     TDEQKADWMPELTAGRKLAAFGLTEPEAGSDAGNTKTRAELDGDEWVVNGAKQFITNS
                     GTDISACVTITAVTGKDENGRPEISNIIVPNGTPGYTVDPPYRKMGWNASDTHPLTFE
                     DARVPESNLLGPRGQGFRQFLQILDGGRIGVAAMSVGVAQGALDEAIKYAKERQAFGQ
                     SISKFQSIQAKIADISAQLEAARLLTYRAAIEKDRGENFSLTAAQAKLITGRLAVRAT
                     EEAVQIHGGYGFIEEYPVCRFYRDAKILTIGEGTDEVQQMVIARALGC"
BASE COUNT          311 a          646 c          704 g          352 t
ORIGIN      
        1 cccggaaccg gcggggcgcc ggaagtgctg gggtcggtcc agtacttctt ccggctgctg
       61 ggcgccgcgg cggctcgcgc gctcgagctc gaatacgggt tgctggttgc cgatccccga
      121 aagggagccg gccagaccgc actcgatggc gcgatcgtgg tcgatccggc gatcgatgac
      181 cccgtagtgg ccgcctgcga cacggccggc ctgcccgtgg tcacgatcgg caggcggctg
      241 gcctccgatg aatccggaaa agccggtacc tgggtggttg acaacgattt ccttgccgcc
      301 accggttcgg tgctcgatca cctggccggc cagggcgcaa ggtcgatcgc cctgatggcc
      361 agcgagccga tcgactcctt tcagcaggac agcatcgatg cctaccggca gtggtgcgcc
      421 gggcacgggg tcgaggcccg ggtcgtcgtc tcgcagtcgc cggacccgaa ggacgcaacc
      481 gtcgcggcgg gccggctatt tgaccagggg caaatgccgg acggggtcta cgcgacgatc
      541 gacactctcg cccgggccct gcttgaccgg gcggcttcag aacgcgtcgc ggtgcccggc
      601 gagctcagga tcgccacctg cagcaacggc gagctggccc gcacctcgca gccccagctg
      661 accacgctgg acgagcatcc ggccacgctc ggggaggaag cggtcgaatt gctggtccgc
      721 gcgatcgaag acccggagtc ggcgccctcc ttcgtgaagg tggacaccga gctcctggtc
      781 agggaatcga ccgcctgagc gaagccctaa gccctccccg gctgcctcag cagcccaggg
      841 cccgggcgat caccatctgc tggacctcgt cggtcccctc gccgatcgtg aggatcttcg
      901 cgtcgcggta gaagcggcag accggatact cctcgatgaa gccgtaaccg ccgtggatct
      961 gaaccgcctc ctcggtcgcc cgcacggcga ggcggccggt gatcagcttg gcctgggcgg
     1021 cggtcaggga gaagttctcg ccgcggtcct tttcgatcgc ggcccggtag gtcagcaacc
     1081 gggcggcctc gagctgggcg gagatgtcgg cgatcttcgc ctggatcgac tggaacttgg
     1141 aaatcgactg gccgaaagcc tggcgttcct tcgcgtactt gatcgcttcg tccagcgcgc
     1201 cctgggcgac tccgaccgac atcgcggcga cgccgatccg gccgccgtcg agaatctgca
     1261 ggaactgcct gaaaccctgg ccgcgcgggc cgagcaggtt cgattcggga acccgggcat
     1321 cctcgaaagt cagggggtgg gtgtccgagg cgttccagcc catcttgcgg tagggaggat
     1381 cgacggtgta accgggggtg ccgttgggca cgatgatgtt cgagatctcg ggccggccgt
     1441 tttcgtcctt gccagtcacg gcggtgatcg tgacgcaggc ggagatgtcg gtgccggagt
     1501 tggtgatgaa ctgcttggcc ccgttcacga cccattcgtc gccgtcgagc tcggcccggg
     1561 tcttggtgtt tcctgcgtcg gagccggctt cgggttcggt caggccgaag gcggcgagct
     1621 tccggccggc ggtgagctcg ggcatccaat cggccttctg ctcgtcggtc ccccagttgt
     1681 agatcggggt ggtaccgagc gagatgtggg ccgccatggt gatcgcaacc gacgagtcga
     1741 tccgggccag ttcctcgacc gcgagggcgt aggaaagaat gtcgccgccg ctgcccccgt
     1801 attcctccgg gaaggggata cccatcagcc cgaggtcgcc gagcttggcg acgatctcgt
     1861 aggggaaggc cttggtgcgg tcgagctcct ccgcgacggg aacaacttca ttacgggcga
     1921 aatcgcggac ggtgttctgg aggagacgct gttcgtcggt caggtcgaag ttcatgacgc
     1981 gatcctacga attccttcct cgtgtgagat atc
//
LOCUS       DAOQMF010000362         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38339_length_2012_cov_3.141543, whole genome shotgun sequence.
ACCESSION   DAOQMF010000362 DAOQMF010000000
VERSION     DAOQMF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38339_length_2012_cov_3.141543"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLV77_06600"
     CDS             complement(<1..662)
                     /locus_tag="PLV77_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC15551.1"
                     /translation="MSRDHRKPSRYNQQFFRQWGGPGPLFFGLLALIIVLLGLYLAFT
                     KSIPFTSPGYQVQATFRNAVNIAIKSPVRIAGVNVGKVTDMEQKGDNTVVTFTVDDEG
                     RPVREDASAQIRPRLFLEGNWFIDLDPGTPDSSEMPDEGMIPVSHTGTSVQVGDLLRT
                     LQTPERANIQRLLESLGTGLNSKPTAEQDLTFEPFVQGLSGGEALNLAYRQGEVAARG
                     TA"
     gene            complement(659..>2012)
                     /locus_tag="PLV77_06605"
     CDS             complement(659..>2012)
                     /locus_tag="PLV77_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC15552.1"
                     /translation="VKADLPSAASLVAGNEVRIAGVRVGLISQVKAKQLDDGRSIAVA
                     TMKLDKSVEPIPEDSTVLVRQRSAMGLKYLLLTPGKSDQGLEPGGTLPESQAEEPVDM
                     DQWFNMFQAPVRRAIQKNLAEYGGGLAARGADINQLIADLPPLLRLAEPVAKNLSSKQ
                     TDLRGFVRGLSQAAAEVAPVAETQADMFVQLDRTFIALAEVARPYIQDSITEGVPTEL
                     AVIRNGPRIRPFLYTSADFMKALKPGAKALGESAPIVSRSFDIGIPVLRSSPQLYDEL
                     APTARSLRRFGESTSNNEGLDMLISTNEILTPLLRQVTPSQNTCNYLNLLLANVASAT
                     GGHNSEGNAVRAVSVLPQIGVNAEGNPASGMANGGTIVPPPDDPTNKNYLHSNPYPNT
                     AAPGQSPRECEPGNIPDGWFIPSGGTTWVNALQVGNLDGDQGLITQDQTQAQLNWRTK
                     "
BASE COUNT          326 a          606 c          703 g          377 t
ORIGIN      
        1 atcgctgtgc cgcgggcagc cacttcacct tggcggtagg caaggttcag cgcttcaccg
       61 ccgctcaggc cctggacgaa gggctcgaag gtcaggtcct gctcggcggt cggcttcgag
      121 ttgaggccgg tgccgaggct ctccagcagg cgctggatgt tggcgcgctc gggagtctgg
      181 agggtccgca acaggtcgcc gacctggacg ctggtgcccg tgtggctgac cgggatcatg
      241 ccctcgtccg gcatttccga tgaatccggg gtgccgggat caaggtcgat gaaccagttg
      301 ccctcgagga agagccgggg acggatctgg gccgaggcat cctcgcgaac cggccggcct
      361 tcgtcatcga cggtgaaggt gaccacggtg ttgtcaccct tctgttccat gtcggtcacc
      421 ttgccgacgt tgacgccggc gatgcgaacc ggggacttga tcgcgatgtt tacagcgtta
      481 cggaaggtcg cctggacctg gtagccgggg cttgtgaacg ggatcgactt ggtgaaggcg
      541 aggtaaaggc cgagcaggac gatgatcagc gcgagcaggc cgaagaacag cggaccggga
      601 ccgccccact ggcggaagaa ctgctggttg tagcggctgg gcttgcggtg gtcgcggctc
      661 atttggtccg ccagttcagc tgcgcctggg tctggtcctg ggtgatcagg ccctgatcgc
      721 catcgagatt gccgacctga agtgcgttga cccaggttgt gccaccgctc ggaatgaacc
      781 agccgtcggg aatgttgccc ggctcacatt cgcgcggtga ctggcccggg gctgcggtgt
      841 tcgggtacgg gttcgagtgc aggtagttct tgttggtcgg gtcgtcgggc gggggaacga
      901 tcgtgccacc atttgccatg ccggatgcgg gattgccttc ggcattgacg ccgatctggg
      961 gcagcacgct gaccgcccgc accgcgttgc cctcgctgtt gtggccgccg gtcgccgagg
     1021 ccacgttggc gagcagaagg ttgaggtagt tacaggtgtt ctgcgagggc gtcacctgac
     1081 gcagcagcgg ggtgaggatc tcgttggtgc tgatcagcat gtcgaggccc tcgttgttcg
     1141 aggtgctctc gccgaatcgc ctcagcgaac gggcggtcgg ggccagctcg tcatagagct
     1201 gcggtgagga gcgcagcacc gggatgccga tgtcaaacga cctggaaacg atcggggcac
     1261 tctcgccgag tgccttcgcc cccggcttca acgccttcat gaagtcggcc gaggtataga
     1321 ggaagggacg gatccggggc ccgttgcgga tcaccgcgag ctcggtcggg acaccttcag
     1381 taatcgaatc ctggatgtag gggcgggcca cttcggccag cgcgatgaaa gtgcgatcga
     1441 gctggacgaa catgtcggcc tgggtctcgg ccaccggagc cacctcggct gccgcctgcg
     1501 aaagaccgcg cacgaagccc cgaaggtcgg tctgcttcga ggaaagattc ttcgccactg
     1561 gctcggccag cctgagcagc ggcggcaggt cggcgatcag ctggttgatg tccgccccgc
     1621 gagccgccag gccgccaccg tattcggcca gattcttctg gatcgcccgg cgtaccggcg
     1681 cctggaacat gttgaaccac tggtccatgt cgaccggttc ctcggcctgg gattcgggca
     1741 gggtgccgcc cggctcgagt ccctggtcgg actttccggg agtcaagagc agatacttga
     1801 gtcccatcgc cgagcgctgg cgaaccagca ccgtcgaatc ctcggggatc ggctcgacgc
     1861 tcttgtcaag tttcatcgtc gccaccgcaa tggagcggcc gtcatcgagc tgctttgcct
     1921 tgacctggct gatgagaccg acccggacac cggcgatgcg cacctcgttg cccgccacca
     1981 gcgaagcggc cgaaggtaga tcggccttca ct
//
LOCUS       DAOQMF010000363         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38358_length_2011_cov_4.388037, whole genome shotgun sequence.
ACCESSION   DAOQMF010000363 DAOQMF010000000
VERSION     DAOQMF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38358_length_2011_cov_4.388037"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..611)
                     /locus_tag="PLV77_06610"
     CDS             complement(162..611)
                     /locus_tag="PLV77_06610"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNC15553.1"
                     /translation="MRILFVCLGNICRSPSAEAVMAGLIVREGIEGIEIDSAGTGDWH
                     VGQPADPRAIAAATDRGVEISGLARQVRPSDFEEFDLILAMDSSNHANLLAMDGADPE
                     QVRLFREMAGEGELDVPDPYYGGEDGFAEVLDILESGCAVILEEINA"
     gene            complement(620..1117)
                     /locus_tag="PLV77_06615"
     CDS             complement(620..1117)
                     /locus_tag="PLV77_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15554.1"
                     /translation="MTGKTKTTLAIIFGVVGILLGILGTITAFNAKNAVDDDKSQTSE
                     VAALVEQKFNEAQAKQDQAEANQRSEAEKLVAQLTQGKKNLLQKINTNHRQLKKVRRQ
                     VKNLSGQVSSLQSRDRELQNEIDQIENDQQADYNQLNGRINNLNRNVQQLQKQFARLN
                     GEING"
     gene            complement(1152..1379)
                     /gene="secG"
                     /locus_tag="PLV77_06620"
     CDS             complement(1152..1379)
                     /gene="secG"
                     /locus_tag="PLV77_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNC15555.1"
                     /translation="MYTIMAIIQVLLSVLLIFLVLLHSGKDAGLSGAFGIGGGGSSIG
                     GGSMVEQNLDRATVVTAILFTLNTILLIKLS"
     gene            complement(1392..1670)
                     /locus_tag="PLV77_06625"
     CDS             complement(1392..1670)
                     /locus_tag="PLV77_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15556.1"
                     /translation="MTVVWFIVLLVLVAVFVSMPFWRRGEASGTEDPEIAALEAARES
                     KYREIRDAEMDRESGKLSEEDFRELDAELRREAVGILDRLEALKGKES"
     gene            complement(1667..>2011)
                     /locus_tag="PLV77_06630"
     CDS             complement(1667..>2011)
                     /locus_tag="PLV77_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15557.1"
                     /translation="EPNTDEFQRRARLADGLFGAYMRKKVLPIVQADPAKAETVSRAA
                     ENLLSLTTDKVIAGYNSDSIEVPVTIRVIVSPMVTWIWIGAVIAIIGALFAAWPSGLL
                     TRKRGGKGRKAA"
BASE COUNT          343 a          659 c          609 g          400 t
ORIGIN      
        1 gggaagtaca ggatcgcacg gggcacgacc cgtgtcaccg ggatccggcg caagcggaca
       61 agggcgaagg cgcgggtcat cttcgccaca ccgttcagct gccccggcaa gctcgggaac
      121 tgccgcagct ggcagaacct gccctgaccc gcgcagcgcg gtcaggcgtt gatctcttcg
      181 agaatcacgg cacagccgct ctcgaggatg tccagcacct ctgcgaaacc gtcctcgccc
      241 ccgtagtagg ggtcgggcac atcaagctct ccttcgcccg ccatttcccg gaagagcctc
      301 acctgttcgg ggtcggctcc gtccatcgca agcagattcg cgtggttgga gctgtccatc
      361 gcgaggatca ggtcgaactc ctcgaagtcc gagggccgga cctggcgggc gaggccgctg
      421 atctcgaccc cgcggtcggt cgcggccgcg atcgcccgcg gatcggccgg ctggccgaca
      481 tgccagtcgc cggtgccggc cgaatcgatc tcgattccct cgattccttc gcgcacgatc
      541 agcccggcca tcaccgcctc ggccgagggc gaacggcaga tgttgccgag gcatacgaaa
      601 aggatccgca agtccgggct cagccgttga tctcgccgtt cagccgggcg aactgcttct
      661 gcagctgctg aacgttcctg ttgaggttgt tgatccggcc attcagctgg ttgtagtcag
      721 cctgttgatc gttttcgatc tgatcgatct cgttctgaag ctcgcggtcc cggctctgga
      781 gcgaactgac ttgccccgag aggttcttca cctgccgacg aaccttcttt agctgcctgt
      841 ggtttgtgtt gatcttctgg agcaggttct tcttgccctg ggtcagctgg gcgaccagct
      901 tctcggcctc tgaacgctgg ttggcttcgg cctggtcctg cttggcctgg gcttcgttga
      961 acttctgctc gaccagagcc gcgacttcgc tggtctggct cttgtcatcg tcaaccgcgt
     1021 tcttcgcgtt gaaagcggtg atcgtgccga gaatcccgag caggattccg actacgccaa
     1081 aaatgatcgc cagtgtcgtc ttggtcttgc cagtcatggc gctgacccta gaccaacaac
     1141 ggggatccgg cttaagaaag cttgatcagg aggatcgtgt tcagggtgaa caggatcgcg
     1201 gtgacaaccg tcgcccggtc gagattctgc tcgaccatcg agccgccgcc gatcgaactg
     1261 ccgccgccac cgatgccgaa agcgccggag agacccgcgt ccttgcccga atgcaggagc
     1321 accaggaaga tcaggagcac ggaaagcagg acctggatga ttgccatgat cgtgtacatc
     1381 ggccagcgat tctagctttc tttgcccttg agcgcctcga ggcggtcgag aatgccgacc
     1441 gcttcccgtc tcagttcggc gtcgagttcc ctgaaatcct cctccgagag cttgccggac
     1501 tcgcggtcca tctcggcgtc gcggatctcc cggtactttg actcccgggc cgcttcgaga
     1561 gcggcaattt cgggatcctc ggtgccggaa gcctcgccgc gacgccagaa cggcatcgag
     1621 acgaagaccg cgaccaggac cagcaggacg atgaaccaga cgacggtcac gcggctttcc
     1681 ttcccttccc gccccgtttc cgggtcagga gtccactcgg ccaggccgcg aaaagcgcac
     1741 cgatgatcgc gatcacggcg ccgatccaga tccacgtgac catcgggctg acgatcaccc
     1801 ggatcgtcac cggcacctcg atgctgtccg agttgtatcc ggcgatcacc ttgtcggtgg
     1861 tcaggctcag caggttctcc gccgcacggc tgaccgtttc cgccttggca ggatcggcct
     1921 gaacgatcgg caggaccttc ttgcgcatgt aagccccgaa gagtccgtcg gcaagcctcg
     1981 cccgacgctg gaactcgtcc gtgttcggct c
//
LOCUS       DAOQMF010000364         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38457_length_2009_cov_2.957523, whole genome shotgun sequence.
ACCESSION   DAOQMF010000364 DAOQMF010000000
VERSION     DAOQMF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38457_length_2009_cov_2.957523"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PLV77_06635"
     CDS             complement(<1..1124)
                     /locus_tag="PLV77_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15558.1"
                     /translation="MLGVAVAQAATGDTLGVTTVTQRIVPDSSAGFNFLAAGPGEAYT
                     VRDGSEEGGVSLGSADAGRATRRTSISYFGQLTDFQLADEESPLRVEFLDPQGGAFTS
                     AWRPGEAMNPQEEDAMMRQFNAFASKPPNLAKSGAKPKMDFVVNTGDISDNNQYNEAL
                     WNLQIAEGGTVNPGTGVDPDPYVGEHPLCPAGMNVLNADDPTAYTGVQDRDEWPAPTM
                     GYFWDPDQPDPGPVAVNPFADWPSYPGLMDRSQRPFKATGLKVPSYFVFGNHDNLVQG
                     NAWGSGIFNQIATGCLKPINDDAANSGRSSNPLLSFVLNPNFTAQDFLDIRTGEPKYF
                     MEVPPDPDRHLISKKEAMNLYRSTGKSNGHGFGFVDQASRT"
     gene            complement(1381..>2009)
                     /locus_tag="PLV77_06640"
     CDS             complement(1381..>2009)
                     /locus_tag="PLV77_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC15559.1"
                     /translation="KAQIHAFSHAGQSCISIQRILVHEAVADRFIERLVANVEKLKVG
                     DPSLDDTDVGPLITPGDRDRVKEWIDEAAAGGAKVLTGGELVDEGRCLAPTLIEGPSR
                     EAKVWCEEIFGPVATVDRFSDFDEALARANDSKFGLQAGVFTRDVGNGLKAGNTLEFG
                     GVLINEVPTFRADQQPYGGVKDSGNTREGPAYSIIEMTEERFVTFQAG"
BASE COUNT          321 a          619 c          698 g          371 t
ORIGIN      
        1 gtgcgggaag cctggtcgac gaaaccgaaa ccgtgaccgt tcgacttgcc ggtgctccgg
       61 tagaggttca tcgcttcctt cttcgagatc agatggcggt ccggatccgg cgggacctcc
      121 atgaagtact tgggttcacc agtccggatg tcgaggaaat cctgggcggt gaagttcggg
      181 ttcagcacga agctgagcag cgggttcgac gaacggccgc tgttggcggc atcgtcgttg
      241 atcggcttca ggcagccggt cgcgatctgg ttgaagatgc cgctgcccca ggcgttgccc
      301 tggaccaggt tgtcgtgatt gccgaagacg aagtaggagg ggaccttcag tccggtcgcc
      361 ttgaagggac gctgggagcg gtccatcagg cccgggtagg aaggccagtc ggcgaagggg
      421 ttgacggcaa ccgggcccgg atcgggctgg tccggatccc agaagtaacc catggtcggg
      481 gcgggccact cgtcgcggtc ctgcacgccg gtgtaggcgg tggggtcgtc ggcgttcagc
      541 acgttcatgc cggcggggca gagcggatgc tcgccgacgt acgggtccgg atcaaccccg
      601 gtgcccgggt tcacggtgcc gccctcggcg atctgcaggt tccagagcgc ctcgttgtac
      661 tggttgttgt cggagatgtc accggtgttg acgacgaagt ccatcttcgg cttggcgccg
      721 ctctttgcga ggttcggcgg cttggacgcg aatgcgttga actggcgcat catcgcgtct
      781 tcctcctggg ggttcatcgc ctcgccgggc cgccaggccg aggtgaaggc gcctccctgc
      841 gggtcgagga actcgacccg cagcggactc tcctcgtcgg cgagctggaa gtcggtgagc
      901 tgcccgaagt aggagatcga ggtgcggcgg gtggcgcggc cggcgtcggc gctgccgagc
      961 gatacaccgc cctcttcgga gccgtcgcgg accgtgtacg cctcgccggg accggcagcc
     1021 aggaagttga agccggcgga cgagtcgggg acgatccgct gggtcacggt tgtcacaccg
     1081 agcgtgtcgc ctgtcgctgc ctgtgcgact gcaactccga gcaagccgag ggcagccaca
     1141 agagcggcag caactttggt cctgaaccgg ttcatcaatt ctccttgcgg ccggttcccc
     1201 gaaccggcac atgtggtgtg gggctactcg tccggtctcc tccggacggg gacggaatga
     1261 gcctacgtta ccgccatgtg aagaaaccgg ccaggagccc ggatgttgcg ctcacgccgg
     1321 ttcgcttctg ggttgaagtt attcgcacat gggacgaata acttcaaccc ggtcgcgttc
     1381 tcagccggcc tggaaggtga cgaagcgttc ctcggtcatc tcgatgatcg agtaggcggg
     1441 accctcgcgg gtgttgccgg aatccttgac gccgccataa ggctgctggt cggcgcgaaa
     1501 ggtcggcacc tcgttgatta ggacgccgcc gaactcgagg gtgttgccgg ccttcagtcc
     1561 gttgccgaca tcacgggtga agacaccggc ctgcaggccg aacttcgagt cattggcccg
     1621 ggccagcgcc tcgtcgaaat cgctgaagcg gtcgaccgtg gccaccggac cgaagatctc
     1681 ctcacaccag accttggcct cgcgggaagg gccttcgatc agggtcgggg ccaggcagcg
     1741 cccctcgtcg accagctcgc cgccggtcag gaccttcgcc ccgccggccg ccgcctcgtc
     1801 gatccattcc ttcacccggt cccggtcacc gggggtgatc agtgggccca cgtcggtgtc
     1861 gtccagcgag ggatcaccca ccttgagctt ctcgacgttg gcgaccagcc gctcgatgaa
     1921 ccggtcggcc accgcttcgt ggacgaggat ccgctggatc gaaatgcagg actggcccgc
     1981 atgggagaaa gcgtggatct gggccttgt
//
LOCUS       DAOQMF010000365         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38508_length_2007_cov_3.777664, whole genome shotgun sequence.
ACCESSION   DAOQMF010000365 DAOQMF010000000
VERSION     DAOQMF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38508_length_2007_cov_3.777664"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLV77_06645"
     CDS             <1..642
                     /locus_tag="PLV77_06645"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC15560.1"
                     /translation="ITSAAGFNGVVVDMSTIDPGRTTEIAAELAGGGIGFVDAPVSGG
                     EKGAIEGNLSVMAGGSETDFNRALPLLEVVGDRITHCGPAGAGQMVKTCNQLLVGSNL
                     MAIAEAMVLAERSGLVSAATMREAVTGGFADSRCLQVHGQRMVDGTFVPGFKTELHAK
                     DARIVDQMAAQTGTPIRAFEVAREALDRLVESGRGELDDSAVIEIVREEAGEA"
     gene            639..1433
                     /locus_tag="PLV77_06650"
     CDS             639..1433
                     /locus_tag="PLV77_06650"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC15561.1"
                     /translation="MNPLPPVEVEPHPWRGLAWLTRRECHRVLKLWKQTILAPVISSL
                     LFMIVFGLSLGDSIAEIGGFEYRVFIVPGLIAMAMAQSAYSNNSSTIFQARNDRYLDD
                     ILSAPMSAWQVHLGYLAGGAMRSLIIGVVLTALALPLTGAPLEMPLELIVAVVLTIGG
                     FGALGIIVGVYAQSWDNQSFINNIVILPLIFLGGVFYSVSRLGTPWEQISHLNPLFYV
                     VEAVRYGFLGAADVSPWLSLGVVGAITFALLAWAQFLFSTGHRLKP"
     gene            complement(1437..1787)
                     /locus_tag="PLV77_06655"
     CDS             complement(1437..1787)
                     /locus_tag="PLV77_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15562.1"
                     /translation="MAKKRKPKRREREKSSTSSYADAEGNVLVLRDSLGKGSIEKINE
                     QIENQAYSVDDLWQRKTELIFERLAVSWEIAGLPLDDQKMLLGRYRMADPDTRRWVRE
                     ILDQHLRRHIPELA"
BASE COUNT          288 a          684 c          663 g          372 t
ORIGIN      
        1 atcacctcgg ccgccggctt caacggagtc gtggttgaca tgagcacgat cgaccccggc
       61 cgcaccaccg agatcgcggc cgagctggcg ggtggcggca tcggattcgt cgacgctccc
      121 gtttccgggg gcgagaaggg cgcgatcgag gggaaccttt cggtgatggc gggtggcagc
      181 gagaccgact tcaaccgcgc cctgccgctg ctcgaggtgg tcggggaccg gatcacccat
      241 tgcggtccgg ccggagcggg ccagatggtc aagacctgca accagctgct ggtcggcagc
      301 aacctgatgg cgatcgccga ggcgatggtc ctggccgaac ggagcggcct ggtttcggcc
      361 gcgacgatgc gggaggcggt taccggcggt ttcgccgatt cccgctgcct ccaggttcac
      421 ggccagcgca tggtggacgg caccttcgtg cccggcttca agaccgaact tcacgccaag
      481 gatgcccgca tcgtcgatca gatggcggcg cagaccggca ccccgatccg ggccttcgaa
      541 gtcgcccgcg aggcgctcga ccggctggtc gagagtggcc gcggcgagct cgacgactcg
      601 gccgtgatcg agatcgtcag ggaagaagcc ggggaagctt gaatccgctt ccccccgtag
      661 aagtcgagcc gcacccgtgg cgggggctcg cctggctgac ccgacgcgaa tgtcaccggg
      721 tgctgaagct ctggaagcag accatcctcg ccccggtgat ctcctccctg ctcttcatga
      781 tcgtcttcgg gctctcgctc ggtgacagca tcgccgagat cggcggcttc gaataccggg
      841 tcttcatcgt gcccggcctg atcgcgatgg cgatggccca gtcggcctac tcgaacaact
      901 cgagcacgat cttccaggcc cgcaacgacc ggtacctgga tgacatcctc tccgccccga
      961 tgagcgcctg gcaggttcac ctcggctatc tggccggtgg cgcgatgcgc tcgctgatca
     1021 tcggggtcgt cctcaccgcg ctggccctgc ccctgaccgg cgcgccgctg gagatgccgc
     1081 tggagctgat cgtcgcggtg gtcctgacga tcggcggctt cggggcgctg ggcatcatcg
     1141 tcggcgttta cgcccagtcc tgggacaacc agtccttcat caacaacatc gtcatcctgc
     1201 cgctgatctt cctcggcggc gtcttctatt cggtcagccg gctgggcacc ccctgggagc
     1261 agatctccca cctcaacccg ctcttttacg tggtcgaggc ggtccgctac ggcttcctcg
     1321 gggcggccga cgtctccccc tggctatcgc tcggtgtggt cggggcgatc accttcgccc
     1381 tgctcgcctg ggcccagttc ctcttcagta ccggacaccg cctcaagccc tgactttcag
     1441 gcgagttcgg ggatgtgccg gcggaggtgc tgatcgagga tttcccggac ccagcggcgg
     1501 gtgtccggat cggccatccg gtagcggccg agcagcatct tctggtcgtc gagcggaagc
     1561 ccggcgatct cccaggagac ggccagccgt tcgaagatca gttcggtttt gcgctgccag
     1621 aggtcatcga ccgagtaagc ctggttttcg atctgctcgt tgatcttctc gatcgatccc
     1681 ttgccgagcg agtcgcgcag cacgaggacg ttgccctcgg cgtcggcgta actggaggtc
     1741 gaggacttct cccgttcccg ccgctttggc ttccgcttct tcgccatacg cagagtgtgt
     1801 caggccctgg ccgggcgggt tcctgttacc gcccggcgaa gaccgggcgg cgcccttatg
     1861 ttctagcctc cgttgctcgc cggcacgtaa tcccggctcg gctcgccgcg cagcgcggtg
     1921 gccgggggca gcgcggtgcc ctgggcgtcc tggcccggga tcaccggcag gaccagcttc
     1981 gacggaatca cgccgccgag accgatc
//
LOCUS       DAOQMF010000366         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38519_length_2007_cov_2.712090, whole genome shotgun sequence.
ACCESSION   DAOQMF010000366 DAOQMF010000000
VERSION     DAOQMF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38519_length_2007_cov_2.712090"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLV77_06660"
     CDS             <1..571
                     /locus_tag="PLV77_06660"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNC15563.1"
                     /translation="THSSTHVDAPWHYNSTIQGKPAETIDQLPLDWFFRPGFLLDFHE
                     RADGEVVEIADIEAELRRTGHEIAERDICLVRTGCDQYLGTLEYMAHGPGVSAAATRW
                     LYDRGVRVMGIDAWGWDRPLWMQAEEAKEMGEAGIFWEAHQADIPYSQIERLANLDQL
                     PATGFTVACFPLKLVGGSAAPARVVAIID"
     gene            complement(561..>2007)
                     /locus_tag="PLV77_06665"
     CDS             complement(561..>2007)
                     /locus_tag="PLV77_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15564.1"
                     /translation="GSDPGSEYDPAVKSRPFFRLLILGLLALALVPASAGASVAGAAK
                     RLDNLRDQPQRLRQFLLGMPKGTDLHTHLSGAVYAEAMIRWGAEDGKCVDPVTFVSTF
                     PPCTGSQVPLTTALTNTQLWDQILAAWSMREFRPDAFSGHNHFFATFDLFGSAFSGRQ
                     GDGLAQVTQRAASQNVQYVEPLITPQFGATLAIANQVGYIPDFAEMREAMLGAGITNA
                     IPAAGLTASNALGRESDLLGCATEDPQPGCGVLVHFDVQVLRNQLPAVVFAQLLFGME
                     LMKADPNWAGMNMVQPEDAAYSTSDYNLHMRMVRYLRSVYPDESVTLHAGELVPGIVP
                     PDDLRFHIRQAVNIAGADRIGHGADLRWERNPDQILRSMKSKGTCLEANLTSNQQILR
                     LSGKRHPITDYVRAGIKVTLSTDDEGVSRTDLTAQYVKAVRQHGFGYRRLKQFATYGL
                     RCAFLTAAEKKAALADQKQRFRKFEARFPSR"
BASE COUNT          329 a          634 c          713 g          331 t
ORIGIN      
        1 cacccacagc tcgacccacg tcgacgctcc gtggcattac aactcgacga tccagggcaa
       61 gccggccgag acgatcgacc agcttccgct cgactggttc tttcggcccg ggttcctgct
      121 ggacttccac gagcgggccg acggcgaggt ggttgaaatc gccgacatcg aggcggaact
      181 tcggcggacc ggacatgaaa ttgcggaacg ggacatctgc ctggtaagga ccggctgtga
      241 ccagtacctc ggcaccctcg aatacatggc ccacgggccc ggtgtgagcg cggccgccac
      301 gcgctggctc tacgatcgcg gcgtccgggt gatggggatc gacgcctggg gctgggaccg
      361 gccgctctgg atgcaggccg aagaggcgaa ggagatgggg gaggccggga tcttctggga
      421 ggcccatcag gccgacattc cctattccca aatcgagcgg ctggccaatc tcgatcagct
      481 gccggcgacc ggtttcaccg tggcctgctt cccgctcaag ctggtcggcg gcagcgccgc
      541 ccccgcccgg gtcgtggcga tcatcgacta gggaagcggg cttcgaactt ccggaagcgc
      601 tgcttctggt cggccagggc ggccttcttc tcggccgcgg tgaggaaggc gcagcgcagg
      661 ccgtaggtcg cgaactgctt cagccgccgg taaccgaagc cgtgctgccg caccgctttc
      721 acgtactgcg cggtcaggtc ggtgcgggac acgccttcgt cgtcggtcga gagggtcacc
      781 ttgattccgg cccgcacata atcggtgatc gggtggcgct ttccggacag ccggaggatc
      841 tgctggttcg aggtcagatt cgcttccagg caggtcccct tgctcttcat gctgcggagg
      901 atctggtcgg ggttacgctc ccagcgcagg tcggcgccgt ggccgatccg gtcggcaccg
      961 gcgatgttca ccgcctgcct gatgtggaag cggaggtcgt cgggtggcac gatgcccggc
     1021 accagctcgc cggcgtggag ggtcacgctc tcatccggat agaccgacct caggtacctg
     1081 accatccgca tgtggaggtt gtaatccgaa gtcgagtagg ccgcgtcctc gggctggacc
     1141 atgttcatcc cggcccagtt cggatcggcc ttcataagtt ccattccgaa gagaagctgg
     1201 gcgaagacca ccgccggcag ctggttgcgc aggacctgga cgtcgaagtg gaccagcacg
     1261 ccgcagccgg gttgcgggtc ctcggtcgcg cagccgagca ggtcggattc gcgaccgagc
     1321 gcgttggatg cggtaagccc ggcggccgga atcgcgttgg tgatcccggc acccagcatc
     1381 gcctcgcgca tctcggcgaa gtccgggatg tagccgacct ggtttgcgat cgcgagcgtt
     1441 gcgccgaact ggggcgtgat caacggctcg acgtactgga cgttctggga agccgcccgc
     1501 tgggtcacct gggccaggcc gtcaccctgc cggccgctga aggcggaacc gaagaggtcg
     1561 aaggtcgcga agaagtggtt gtgaccggaa aaggcgtcgg gccggaactc ccgcatcgac
     1621 caggccgcga ggatctggtc ccagagctgg gtgttggtca gggccgtggt cagcggcacc
     1681 tggctgccgg tgcagggcgg gaaggtcgag acgaaggtga ccgggtcgac gcacttgccg
     1741 tcctcggcgc cccagcggat catcgcctcc gcgtaaaccg cgccggagag gtgggtgtgg
     1801 aggtctgtgc ccttcggcat cccgagcagg aactgccgga ggcgctgcgg ctggtcacgc
     1861 aggttgtcca gccgcttggc cgccccggcc accgaggcgc cggcactggc cggaaccagg
     1921 gcgagggcca gcaggccaag gatcagcagc cggaagaagg gacgggactt cacagcagga
     1981 tcgtattcgc tgccgggatc agatccc
//
LOCUS       DAOQMF010000367         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38639_length_2004_cov_3.107748, whole genome shotgun sequence.
ACCESSION   DAOQMF010000367 DAOQMF010000000
VERSION     DAOQMF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38639_length_2004_cov_3.107748"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLV77_06670"
     CDS             complement(<1..408)
                     /locus_tag="PLV77_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15565.1"
                     /translation="MRNPRITLCGRLNVNWDGEDLGESLPGRQGRLLFAYLALNRHRP
                     VRRDELVEALWAEEGLPSGGEALLAPPLSRLRKALGPGRLEGRSELELALGDDAWVDW
                     EAAQAGIEEARLDESAGRHREAYELAGEAEKILS"
     gene            471..1769
                     /locus_tag="PLV77_06675"
     CDS             471..1769
                     /locus_tag="PLV77_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC15566.1"
                     /translation="MSTPSLINLLPAAARMAPETPEEVASALVEAGRQSRKVVVFSTG
                     HGIPPIGDTSDRVLLDMSAFDKVEIDPLARTARIGGATQWMELISAANEHGLTAPFGS
                     AATVGVTGYMMGGGVGFMSRHLGLGSSAIRSAELVTPDGRIRRIDAGSDPDLFWALRG
                     GGGGFGVITELEIELTPVPRMAGGVLGWGIEDAERVLGAWSEWTRRIDDDVTSAVRFM
                     HPPEGPALTVVVVTAPRAAEELAAILKPLTDLKPMLNTVRDTDPEAFLREYTDPEIDP
                     AEPPHAFEHVLLDRLPPEAVQLTAEFAQPVTGCGAMMVEIRHMGGALGRPAPGGGASD
                     RIDGEYLFFVVGFPDVAETMNHAVDVLSDFGRGRTYFNFMPRRTGRETAYGQEAIARL
                     ADLYEAVDPEGLIDHPHPISRTASVADETATTQVLAILHG"
BASE COUNT          314 a          732 c          647 g          311 t
ORIGIN      
        1 ggaaaggatc ttctcggctt cgcccgccag ctcgtaggcc tcccggtgcc gaccggccga
       61 ttcatccagg cgagcctctt cgatcccggc ctgggctgcc tcccaatcga cccaggcatc
      121 atcgccgagg gcgagctcga gttcggagcg gccctcgagc cgccccgggc caagggcctt
      181 gcgcaggcgc gaaagagggg gcgccagcag tgcctctccg ccacttggca agccctcttc
      241 ggcccaaagc gcctcgacca gttcatcccg tctgaccgga cggtgccggt tcagcgcaag
      301 gtacgcaaaa agcagcctgc cctggcgccc cggcagggat tcgccgagat cctcgccgtc
      361 ccagttcacg ttcaaacggc cgcaaagtgt gatccgggga tttctcatcg caaggtgaca
      421 gtcatttcgc cgcaatccgg gcgcaacctg tgccgtggat tctgccagcc atgtcaacgc
      481 cttctctcat aaacctcctt cccgcggcgg cccgcatggc gccggaaacc cccgaagaag
      541 tcgcctcggc cctcgtcgaa gcgggccgcc agagtcgcaa ggtggtcgtc ttctcgaccg
      601 ggcacggcat cccgccgatc ggtgacacct cggatcgcgt cctgctcgac atgagcgcat
      661 tcgacaaggt cgagatcgat ccgctcgccc ggaccgcccg gatcggcggc gccacccagt
      721 ggatggagct gatctcggcc gccaacgagc acgggctcac cgcaccgttc ggatcggccg
      781 ccacggtcgg cgtcaccggt tacatgatgg gtggcggcgt cggcttcatg tcccgccacc
      841 tggggctggg cagcagcgcg atccgctcgg ccgaactggt cacgcctgac ggccggatcc
      901 gccgcatcga cgccggatcc gacccggacc tgttctgggc cctgcgcggg ggcggtggcg
      961 gcttcggcgt gatcaccgag ctcgagatcg aactgacccc cgtgccgcgg atggccggcg
     1021 gagtgctcgg ctggggcatc gaggacgcgg agcgggtgct cggcgcgtgg tccgaatgga
     1081 cccgccggat cgacgacgac gtgaccagcg cggtccgctt catgcatccg cccgaagggc
     1141 cggccctgac cgtggtggtc gtgaccgccc cccgcgcggc ggaagagctg gccgcgatcc
     1201 tgaagcccct gaccgacctg aagccgatgc tgaacacggt ccgggacacc gaccccgaag
     1261 ccttcctgcg ggagtacacc gacccggaga tcgatccggc agagccgccc catgccttcg
     1321 agcacgttct gctcgatcgg cttcccccgg aggcggtcca gctgaccgcc gagttcgccc
     1381 agccggtgac cggctgcggg gcgatgatgg tggagatccg ccacatgggg ggagcgctgg
     1441 gtcgaccggc ccccggcggc ggcgccagcg accggattga cggcgagtac ctgttcttcg
     1501 tggtcggttt ccccgatgtg gcggagacca tgaaccacgc cgtcgatgtc ctctcggact
     1561 tcggccgcgg tcggacctac ttcaacttca tgccccggag gaccggtcgc gagaccgcct
     1621 acgggcagga agcgatcgcc cggctcgccg acctctacga agccgtcgat ccggagggtc
     1681 tgatcgacca tccccacccg atttcccgca ccgccagcgt cgccgacgaa accgccacga
     1741 cccaggtcct ggcaatcctc cacggctgac agtcgcccgg cggggcaacc tcaaacgcag
     1801 aaatacaggc gcccggcggt ctctccctga ccaccgggcg cttttcgctt tcaggttcgt
     1861 gctttgatcg gtggccgtga ccctgcgccc gacaatcgac ctgctgctcg aggacgagca
     1921 gatctcccgc ctctcggccg aggtccgcga ggccgtcgcc ggttccgccg gaacaacggt
     1981 ccgggcggaa gtctccgcca ccct
//
LOCUS       DAOQMF010000368         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38671_length_2003_cov_3.092402, whole genome shotgun sequence.
ACCESSION   DAOQMF010000368 DAOQMF010000000
VERSION     DAOQMF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38671_length_2003_cov_3.092402"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1099)
                     /locus_tag="PLV77_06680"
     CDS             complement(182..1099)
                     /locus_tag="PLV77_06680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC15567.1"
                     /translation="MPASPTATPEHIKDVNTRYHDAAADSYDAKWGIDFGDIGQHQVA
                     LKLKKALGELPRTLGDTLEIGAGTGYFSLNLATQGLIESLTATDISPGMLKSLARTAG
                     SLNFPVETVVTEAEVLPFEDESFDTVIGHAVLHHIPDLDQAFSEFFRVLRPGGRIVFC
                     GEPSRYGDRLAAVPKRTGNLVAPAWRKLVGADTLEHTEEEKADDEHSLEPEVDVHAFV
                     PADLRAIPPRSGFVETRVSGEELVANVWGWGMRSMESTATPDSIPLRWRNFAFRSYIA
                     FQKVDNNLLEPHLPAELFYNLLLSARKPA"
     gene            1131..1883
                     /locus_tag="PLV77_06685"
     CDS             1131..1883
                     /locus_tag="PLV77_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC15568.1"
                     /translation="MTDTYIQSNQPEGVPALELTGERTLPDVPEENYWYRRHLVVYEW
                     IAERVRGLKVADLACGEGYGSGILGTAAAEVIGVDANPEAFEHASAKYTTDRVTFRRE
                     LVESFSEPRDAIVFLQTIEHVQDPDSLVAGFAKTAPVAYISTPNLLTLAPEGAEKSDN
                     PWHIKEYRLEEFRALLEPHFARVEILGLHHAGKLKVHELALSLGWDRVHKSLGLSKPF
                     YDRFTPAIDASDFRLSNHNLESSLDFLAVCHV"
BASE COUNT          371 a          624 c          659 g          349 t
ORIGIN      
        1 cgggcggcgc cagaaacacc aggacgacgc cgcacttcgt caacggccgg aaacggcagc
       61 tcaagtaccc ggccgaagtc cggcaaaggt gccgcaaaaa taggaccatc aagggtctaa
      121 acgtgcggca cctcatgaag cgagggcaag atccgacccc tcgaggcggt agagcgaccg
      181 gctaggcggg tttgcgcgcc gaaagcagga ggttgtagaa gagctcggcc gggaggtggg
      241 gttcgaggag gttgttgtca accttctgga aggcgatgta gctcctgaag gcgaagtttc
      301 gccagcgcag cgggatcgaa tcgggcgtgg cggtggattc catcgaacgc atcccccagc
      361 cccagacgtt ggcgaccagt tcctcgccgc tcaccctggt ctcgacgaaa ccggaccttg
      421 gcgggatcgc ccgcaggtcg gccgggacga aggcgtgaac atcgacctcg ggctcgagac
      481 tgtgttcatc gtcggctttc tcctcctcgg tgtgttcgag ggtgtcggcg ccgaccagct
      541 tgcgccaggc cggggcgacg aggtttccgg ttcgcttggg aactgcggcc agccggtctc
      601 cgtagcggga aggctcgccg cagaagacga tccggccacc cgggcgcagc acccggaaga
      661 actcggagaa ggcttggtcg aggtcgggaa tgtgatggag cacggcgtga ccgatcaccg
      721 tgtcaaagct ttcgtcctcg aaggggagca cctccgcctc ggtgaccacg gtttcgaccg
      781 ggaagttcag ggacccggcg gtgcgggcca gggacttcag catgcccggc gaaatgtcgg
      841 tcgcggtgag gctttcgatc agcccctggg tcgccaggtt gagcgagaag tacccggtgc
      901 cggcgccgat ttcgagggtg tccccgagcg tcctcggcaa ttcgccgagc gccttcttca
      961 gcttcagggc gacctggtgc tgcccgatat cgccgaagtc gatcccccac ttggcgtcgt
     1021 aggagtcagc cgccgcgtcg tggtagcggg tgtttacgtc cttgatgtgt tccggcgttg
     1081 cggtcgggga agcgggcatg gcggagaaag atagggaaga atcggtttcc ttgactgaca
     1141 cgtacatcca gagcaaccag cccgaaggag tgccggccct cgaattgacc ggtgaacgga
     1201 ccctgccgga cgtgccggag gagaactact ggtaccggcg gcacctcgtc gtctacgagt
     1261 ggatcgcgga gcgggtccgg ggcctgaagg tcgccgatct ggcctgcggc gaaggttacg
     1321 gctcgggcat tctcggcact gccgccgccg aggtgatcgg cgtcgacgca aaccccgaag
     1381 ccttcgagca cgccagtgcc aagtacacga cggacagggt cactttccgg cgggaacttg
     1441 tcgagagctt cagcgagccg cgcgatgcga tcgtcttcct gcagacgatc gaacatgtcc
     1501 aggatccgga ctcactggtc gccggctttg cgaagaccgc accggttgcc tacatctcga
     1561 ccccgaacct gctcacgctc gcacccgagg gggcggagaa gtcagacaac ccctggcaca
     1621 tcaaggagta ccggctcgag gaattccggg ccctgctcga acctcatttc gcccgggtcg
     1681 agatcctcgg tcttcaccac gcaggcaagc tcaaggtcca tgaactggca ctgtcgctcg
     1741 gctgggaccg cgtccacaaa tcgctcgggt tgagcaagcc gttctacgac cgcttcaccc
     1801 cggcgatcga cgcctcggat ttccgcctct ccaaccacaa cctcgaaagc tcgctggact
     1861 tcctcgctgt ctgtcatgtc tgaaggaaaa accctccccg gggcgggcga cctggcgatc
     1921 gtccttcatt cgcacatgcc ctacgtcgag ggtttcggca cctatccctt cggggaagag
     1981 tggctcttcg acgcagtgat ccg
//
LOCUS       DAOQMF010000369         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38703_length_2002_cov_3.893169, whole genome shotgun sequence.
ACCESSION   DAOQMF010000369 DAOQMF010000000
VERSION     DAOQMF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38703_length_2002_cov_3.893169"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="PLV77_06690"
     CDS             complement(<1..1291)
                     /locus_tag="PLV77_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC15569.1"
                     /translation="MEEEILVGAPTPAAGPVPKEMAEAEAAYRPEVLGKVLAVVAAAV
                     ALASLDMFIVNIAFPAIEADFPGSSIGSLSWVLNGYVIVFAALLMPFGRLADRVGRKR
                     IFTTGVLIFCLASGACAAAWSVETLVAFRVIQGIGAAMLMSTSLALLLHAFPAERWPV
                     VIGVWAAAGGAAGALGPPIGGLLVEISWRWVFLVNLPLSLAAWYFGTRLLVESKDPRE
                     TRWPDPAGMALMILGVGVLCWGLVEAPDHGWGSIRTVVAILAGLVMLGLTFLRSRRPA
                     DRWIPTLDVDLFKARPFAMACLTALIFMVAFAAILLGGVLFLTTVWGFSVLEAGIAFF
                     PGPAMAGIFSVLGSRFGARFGTGPVAATGCGFLILGGTWWFTRMGDTSAYFTENLPGQ
                     ILVGIGVGLVLPNVAAAVASTLPPTSLATGTAVLSASR"
     gene            1412..1891
                     /locus_tag="PLV77_06695"
     CDS             1412..1891
                     /locus_tag="PLV77_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC15570.1"
                     /translation="MLDREYDNQVCSIARSLEVVGERWSLLIIRNVGLGLNRFEPLRE
                     NLGITRSVLTTRLNTLIEYGILEKRQYSEKPPRFEYHLTQKGRDLSPVLLQLMWWGDE
                     YYPEPGGPPTIAVHRTCGGNLDHHMVCEKCGESVEPDRVYMKPGPGRRRQDLPKIPA"
BASE COUNT          405 a          713 c          619 g          265 t
ORIGIN      
        1 gacgggaagc gctcagaact gccgtcccgg tcgccagcga ggttggcggc agggttgaag
       61 ccacagctgc tgcgacgttc ggcaggacga gtccgacccc gatgccgacc aggatctgtc
      121 cgggcaggtt ctcggtgaag taggcggatg tatcccccat ccgggtgaac caccacgttc
      181 ccccgaggat caggaacccg cagccggttg cggccaccgg cccggtgccg aaccgggctc
      241 cgaaccggct gccgagaacc gagaagatcc cggccatagc cggccccggg aagaaggcga
      301 tccccgcttc cagcacggag aaaccccaaa cagtggtcag gaacaggacc ccgccgagga
      361 gtatcgcggc gaaggccacc atgaagatca gcgcggtcag acaggccatc gcgaagggcc
      421 tggccttgaa gagatccacg tccagggtcg ggatccagcg atcggccggc cgtcgggagc
      481 gcagaaaggt cagtcccagc atgaccaggc cggcgaggat cgcgacgacg gtccggatgc
      541 tgccccaacc atggtccggc gcttcgacca gtccccagca gagcaccccg acgccgagga
      601 tcatcagggc catgccggcc gggtcgggcc acctggtttc gcgtggatcc ttcgactcga
      661 ccagcagccg cgtcccgaaa taccaggctg cgagcgaaag cggcaggttc acgaggaaga
      721 cccatcgcca ggagatttcg accagcaaac caccgatcgg cggaccgaga gcgccggccg
      781 cgccgcccgc cgccgcccag acgccgatca cgaccggcca gcgctccgcc ggaaaggcgt
      841 ggagcagcag ggccagcgaa gtggacatca gcatcgccgc gccgatcccc tgaatgaccc
      901 ggaaggcgac cagggtctcg accgaccagg ccgcggcgca ggctccggaa gcaagacaga
      961 agatcaacac cccggtcgtg aagattcgct tgcgcccgac ccggtcggcc agccggccaa
     1021 agggcatgag caaagcggcg aagacgatca cgtacccgtt gaggacccag gaaagcgagc
     1081 cgatcgaact gccgggaaag tcggcttcga tggccgggaa ggcgatgttg acgatgaaca
     1141 tgtcgaggct ggccagggcc accgccgctg ccacgaccgc cagcaccttg ccgaggacct
     1201 cgggccggta ggccgcttcc gcctccgcca tctccttcgg caccgggcct gcggccgggg
     1261 tcggagcacc gaccaggatt tcctcttcca ctgcctcgaa ctttccggtc acggtgtccg
     1321 acatgttcac ttgtcccgag tgagtatctt catgcgactg accctaacac aatcgggtcg
     1381 catgatgcga ctgacccgct acaatcggtc catgctcgat cgcgagtacg acaaccaggt
     1441 ctgttcgatc gcccgttcgc tcgaggtggt cggtgagcgc tggtcactcc tgatcatcag
     1501 gaacgtcggc ctcggcctga accgtttcga gccgctccgc gagaacctcg ggatcactcg
     1561 cagcgtcctg accacgcgcc tcaacaccct gatcgaatac gggatcctcg agaagcgcca
     1621 gtacagcgag aagcccccgc gcttcgaata ccacctgacc caaaagggcc gcgacctctc
     1681 ccccgtcctg ctgcagctga tgtggtgggg tgacgagtac taccccgagc ccggcggccc
     1741 tccgaccatc gccgtccacc gcacctgcgg cggcaacctc gaccaccaca tggtctgcga
     1801 aaagtgcggc gaatcagtcg agccggaccg ggtctacatg aaacccggcc ccggccgccg
     1861 ccggcaggac ctccccaaaa tccccgcctg aacgccacac cagcccgtca ggcccggagg
     1921 tgccgcacat ccagcaccgt gatgggcctg ttcgtgcgac acctccacga ccagcccgac
     1981 ccgagcaccg cgagatccca ct
//
LOCUS       DAOQMF010000370         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38704_length_2002_cov_3.890601, whole genome shotgun sequence.
ACCESSION   DAOQMF010000370 DAOQMF010000000
VERSION     DAOQMF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38704_length_2002_cov_3.890601"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLV77_06700"
     CDS             <1..348
                     /locus_tag="PLV77_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNC15571.1"
                     /translation="RVVEVIVLEVLQAQLEAQIVQLEERLRRSTVVEEGSAEKGEVSF
                     GSIVHIKDQDSGDSKKFQIVGSTEADLAQGKLSSESPIGAALIGGKVNQIVKIPAPRG
                     PEKQFKITKIEAA"
     gene            373..1872
                     /gene="lysS"
                     /locus_tag="PLV77_06705"
     CDS             373..1872
                     /gene="lysS"
                     /locus_tag="PLV77_06705"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNC15572.1"
                     /translation="MSEAENQEAGTEEGQVEGFAGILAERREKLDRLRAAGVDPFPHS
                     FSPREEISEVIAAHEGLEAGVETEAVHRVAGRISGRRGHGKAAFIDLRDASGQIQLHA
                     KADVLGDQYALLDDLDLGDFVGVEGRAIVTRRGQLSLEVTSWQVLTKALRPPPDKFHG
                     LEDTETRYRRRELDLIANEESREIFRVRARTVSEVRRWLDDHGFFEVETPVLQPLYGG
                     ALARPFVTHHNALDRQLFLRIATELYLKRCVVGGIDKVYELGKDFRNEGISMKHNPEF
                     TMLEWYEAYADYSDTAAELEELVSGVAEKVLGKAEIERDGRTISLAAPWHRVTLRDAI
                     LEHSGIDIDELTDRDALSEAIGNEDETAGWGKLVDTVFSKRVEPRLVEPTFILDYPEE
                     LSPFAKQHRDKPGKVERWEAFIGGVEIANSFTELNDPDEQRRRFEQQAAEIDRGDEEA
                     QPFDESFIEALEQGMPPTGGVGLGIDRLVMMLTGAGSPREVVLFPAMRD"
BASE COUNT          370 a          659 c          664 g          309 t
ORIGIN      
        1 cgcgtcgtcg aagtaatcgt acttgaagta ctgcaggccc agctcgaagc gcagatcgtc
       61 cagctcgagg agcgcctgcg ccgctcgact gtcgtggaag agggttcggc cgagaagggc
      121 gaagtctcgt tcggctcgat cgtccacatc aaggatcagg actcgggtga ctcgaagaag
      181 ttccagatcg tcggttcgac cgaggccgac ctcgcccagg gcaagctttc gagcgagtcg
      241 ccgatcggcg ccgccctgat cggtggcaag gtcaaccaga tcgtcaagat cccggccccg
      301 cgcggtccgg agaagcagtt caagatcacc aagatcgaag ccgcctgatc aactgaccgg
      361 aaagggccgg cgatgagcga agccgaaaac caggaggccg ggaccgaaga gggccaggtc
      421 gagggtttcg ccggcatcct cgccgagcgt cgcgagaagc tggaccgcct gcgtgccgcg
      481 ggggtcgatc ccttcccgca ttcgttttct ccccgtgagg agatcagcga ggtaatcgcc
      541 gcccacgaag gcctcgaagc cggcgtcgaa acggaagcgg tgcaccgggt cgccggccgg
      601 atcagcggcc gtcgcggcca cggcaaggcg gccttcatcg acctgcggga cgcgtccgga
      661 cagatccagc tccacgccaa ggccgacgtg ctcggagatc agtacgcgct gctcgacgac
      721 cttgacctcg gtgacttcgt cggggtcgag ggccgggcga tcgtgacccg ccgcgggcag
      781 ctttcccttg aagtcacctc ctggcaggtc ctgaccaagg cactcaggcc gcccccggac
      841 aagttccacg ggctcgagga caccgagacc aggtatcgcc gccgcgaact cgacctgatt
      901 gccaacgaag agtcacgcga gatcttccgc gtccgtgccc gcaccgtgag cgaggtccgc
      961 cgctggctcg acgaccacgg attcttcgag gtcgagacac cggtcctcca gccgctttac
     1021 ggcggtgccc tggcccggcc tttcgtgacc catcacaacg ctctcgaccg tcagcttttt
     1081 ctgcgcatcg cgaccgagct ctacctcaag cgttgcgtgg tcggcggcat cgacaaggtc
     1141 tatgaactgg gcaaggactt ccgcaacgag ggcatctcga tgaagcacaa ccccgagttc
     1201 acgatgctcg agtggtacga ggcctacgcg gattacagcg acaccgcggc cgagctcgag
     1261 gaactggtct ccggggtcgc cgaaaaggtg ctcggcaagg cggagatcga gcgggacggc
     1321 cgaacgatca gcctcgccgc gccatggcac cgggtcaccc tccgcgacgc gatcctcgag
     1381 cattcgggga ttgacatcga cgagctgacc gatcgggatg ccctttcgga ggcgatcggc
     1441 aacgaagacg agaccgccgg ctggggcaag ctcgtggaca cggtcttctc gaaacgggtc
     1501 gagccccggc tggtcgaacc caccttcatc ctcgattacc cggaagagct ttctcccttc
     1561 gccaagcagc accgggacaa gccggggaag gtcgagcgct gggaggcctt catcggcggt
     1621 gtcgagatcg ccaattcgtt caccgagctg aacgacccgg acgagcagcg gcgccgcttc
     1681 gaacagcagg cggccgagat cgaccggggc gacgaagagg cccagccctt cgacgagtca
     1741 ttcatcgagg cgctggaaca gggcatgccg ccgaccggcg gtgtcgggct cggcatcgac
     1801 cgcctggtga tgatgctgac cggtgccggc tccccccggg aagtggttct cttcccggcg
     1861 atgcgggact gatcgaccgg ccgcggggcg tggccccgac cctcaggccg gttcgccgtt
     1921 cgggcccttg ggcaggcccg gtccgaagta gccggccgaa tggtagatcc ggatcagctg
     1981 cctgatgccg cgccggtagt cg
//
LOCUS       DAOQMF010000371         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38762_length_2001_cov_2.351490, whole genome shotgun sequence.
ACCESSION   DAOQMF010000371 DAOQMF010000000
VERSION     DAOQMF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38762_length_2001_cov_2.351490"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLV77_06710"
     CDS             complement(<1..378)
                     /locus_tag="PLV77_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15573.1"
                     /translation="MDRKVGAALAVAIIGVIVGVVGIVMAKDAKDANQDTQAQLEQQI
                     KQSNKSSAAKVAAAAAAAKVIENKAASKAAESEKQDESSLKSAESKDSSQASANASDI
                     QTLEDKVDELSTEIGNVSGQLKSN"
     gene            478..1995
                     /locus_tag="PLV77_06715"
     CDS             478..1995
                     /locus_tag="PLV77_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNC15574.1"
                     /translation="MGKGSFMLVLLVGLTIAFGSATSAASLPRLKADPDTARGGRIVD
                     TKGREVILRGVNVNSLGQYWQGTSKPPTLPLEKKDPARIAAIGWNLVRLIVSWSRVEP
                     RPGVINTAYLDRVARWTGLMEEEGIYVMLDFHQDAWGPTLAAPDGQVCTPPAQPAFGW
                     DGAPGWATLDDGQPRCFTSNREINPAVRAAWTNFFDDKPASDGVGIQTHYVRMLKRVA
                     KRFAKDPGVAGIDIMNEPNAFGPVQTEQLGRFYGRSLKSIRAGEKAGGGFRHLVFFEP
                     GVLWSLLGSGAPGPFNFDSNVVYSPHLYGGSIGATGPPSRESFENARAEAAGFGGAPV
                     LTGEWGGDPLRADGTGEDYFSQHLDLQDEFRIGSVLWTWKQSCGDPHAATHDPDTAPP
                     LPPWSVYRMDCEGNSNRIAGQYGKLVRSLRRAYVRRAPGRLASMSFDPDTGRLRASGK
                     GARRTDGPLEVYLPLTRARLTLKGLNRVHAKKVGPGMVVWLAPKGGPWELRAKVG"
BASE COUNT          299 a          669 c          668 g          365 t
ORIGIN      
        1 gttgctcttg agctgccccg agacgttgcc gatctcggta ctcagctcgt caaccttgtc
       61 ttcgagggtc tgaatgtccg aggcgttggc tgacgcctgg gacgaatcct tcgactcggc
      121 tgacttcagc gaactctcgt cctgcttctc ggactcggct gccttgctgg cggccttgtt
      181 ctcgatcacc ttcgctgccg ctgcggccgc ggcgaccttg gccgccgagc tcttgttgct
      241 ctgcttgatc tgctgttcga gctgagcctg ggtgtcctgg tttgcgtcct tggcgtcctt
      301 ggccatgacg atcccgacca caccgacgat cacgccgatg attgcgacgg ccagggctgc
      361 tccgactttg cgatccattt cgtttccttc cccggaggac tgaccgccgg aatgcgagtt
      421 tggactggct gccgggactg tgacagccgg acctgacggc tggactacac tgggccgatg
      481 ggcaagggga gtttcatgct ggtgctgctg gtcggcctga cgatcgcgtt cggatccgca
      541 acctcggcgg ccagcctgcc gcggctcaag gccgatcccg acaccgcgcg cggtggccgg
      601 atcgttgaca ccaagggccg ggaggtgatc ctccgcgggg tcaatgtgaa ttccctcggc
      661 cagtactggc agggcacctc gaagccgccc accctgccgc tcgagaagaa ggaccccgcc
      721 cggatcgccg cgatcggatg gaacctggtc cggttgatcg tctcctggtc gcgggtcgaa
      781 ccgaggccgg gggtgatcaa caccgcctac ctggaccggg tcgcgcgctg gaccggcctg
      841 atggaagagg agggcatcta cgtgatgctc gattttcatc aggatgcctg ggggccgacc
      901 ctggcggccc ccgatggcca ggtctgcact cccccggctc agcctgcctt cggctgggac
      961 ggcgccccgg gctgggcgac tctcgatgac ggccagcccc gctgcttcac cagcaaccgc
     1021 gagatcaatc cggccgtgcg cgccgcctgg accaatttct tcgatgacaa gccggcctcg
     1081 gacggggtcg ggatccagac ccactacgtg cggatgctaa agcgggtagc gaagcggttc
     1141 gcgaaggacc ctggcgtcgc cgggatcgac atcatgaacg agcccaacgc cttcggcccg
     1201 gtccagaccg aacagctcgg ccggttctac ggtcgttcgc tgaagtcgat ccgggccggc
     1261 gagaaggcgg gcggcggctt ccggcatctc gtcttcttcg aaccgggggt gctctggtcg
     1321 ctgctcggca gcggcgctcc cggccccttt aacttcgact cgaacgtcgt ctattcgccc
     1381 catctctacg gggggagcat cggcgcgacc ggtccgccga gccgcgagtc atttgagaat
     1441 gcccgggccg aggcggcggg cttcggcggc gccccggtac tgaccggtga atggggtggc
     1501 gatccgctcc gggccgatgg caccggggag gactacttca gccagcacct cgaccttcag
     1561 gacgagttcc ggatcggttc ggtgctctgg acctggaaac agtcctgtgg cgacccccac
     1621 gccgccaccc atgaccccga cacggcgccg ccgctgccac cctggtcggt ttaccggatg
     1681 gactgcgaag ggaattcaaa tcgaatcgcg gggcagtacg gaaagctggt caggtcactg
     1741 cgccgggcct acgttcgccg ggcaccgggc cggctcgcct cgatgagctt tgatcccgac
     1801 accggccgtc tcagggcgag cgggaagggc gcccgccgca ccgacggtcc tctggaggtt
     1861 tacctgcccc tgacccgggc acgcctgacc ctgaagggac tgaaccgggt tcacgcgaag
     1921 aaggtcggcc ccggcatggt cgtctggctc gcgcccaagg ggggcccgtg ggagctccgg
     1981 gccaaagtcg gctgaaggtt t
//
LOCUS       DAOQMF010000372         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38795_length_2000_cov_2.689974, whole genome shotgun sequence.
ACCESSION   DAOQMF010000372 DAOQMF010000000
VERSION     DAOQMF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38795_length_2000_cov_2.689974"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLV77_06720"
     CDS             complement(<1..391)
                     /locus_tag="PLV77_06720"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNC15575.1"
                     /translation="MSWQLVSVVVLLFTLGAGMLWYERSRPPSQIVALVAVLAALAVA
                     GRIVLAPIPNVVATTDIVIIAGFVLGPAPGFAVGALGGLVSNFWLGQGVWTPWQMVAW
                     GMCGVASGVLFHLARGRVNRWTLAAFCG"
     gene            complement(388..1104)
                     /locus_tag="PLV77_06725"
     CDS             complement(388..1104)
                     /locus_tag="PLV77_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC15576.1"
                     /translation="MEAKRLWVELDPGDGPVDVLRGIDLEVQAGERVALMGRNGAGKS
                     TLLRAAAGLIDPVSGRIRAPGGIALLTQNPGDFLVRERVGDELSGPAGEAALGAVGLD
                     VSPDRDPRDLSGGERQRLALAIALAGRMEGEAVPGLVCLDEPTRGMDRGLKGHLTELA
                     GRLSGQGAAVVISTHDVEFAVSFADRVVLLGDGEVVADAEMPTVLSGGWYFASEAARV
                     LDQPGLVSVEQGIEFLKRET"
     gene            complement(1516..1821)
                     /locus_tag="PLV77_06730"
                     /pseudo
     CDS             complement(1516..1821)
                     /locus_tag="PLV77_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          389 a          696 c          597 g          318 t
ORIGIN      
        1 gcccgcagaa ggcggccagg gtccagcgat tgacccttcc ccgtgccaga tgaaagagga
       61 caccgctagc cacaccgcac atgccccagg caaccatctg ccagggcgtc cagactccct
      121 ggccgagcca gaagtttgag accagcccgc ccagggcacc gaccgcgaac cccggggccg
      181 gcccgagcac gaagccggcg atgatcacga tgtcggtggt cgcgaccacg ttcgggatcg
      241 gcgcgagcac aatccggccg gccaccgcca gcgcggccag cacggcgacc agggcgacga
      301 tctgggacgg cgggcgcgaa cgctcgtacc agagcatccc ggccccgagc gtgaacagca
      361 acaccaccac gctgaccagc tgccagctca tgtttccctc ttcaggaact cgatcccctg
      421 ctcgaccgaa accaggccag gctggtcgag tacccgggcg gcctcggagg cgaagtacca
      481 gcctccgctg agcacggtcg gcatttcggc gtcggccacc acctcgccgt caccgagcag
      541 gacgacccga tcggcgaatg aaaccgcgaa ttcgacgtca tgggtcgaga tcacgaccgc
      601 ggcgccctga ccggacaggc gtccggcaag ttcggtcaag tgtcccttga gcccccggtc
      661 cattccgcgg gtcggctcgt cgaggcagac caggccgggc accgcctcac cttccatgcg
      721 tccggccagg gcgatcgcca gggccagccg ctgacgctcg ccaccggaaa gatcgcgcgg
      781 atcccggtcg ggtgagacat ccagaccgac cgcgcccagg gccgcctccc cggccggacc
      841 tgaaagttcg tcgccaaccc gctcgcggac caggaaatca cccgggttct gggtcagaag
      901 cgcgatcccc cccggtgctc tgatcctgcc cgagaccggg tcgatcaagc cggcagcggc
      961 ccgcagcaaa gtcgacttgc cggccccgtt ccggcccatc agggccaccc tctccccggc
     1021 ctgtacttcc aggtcgattc cgcgcagcac gtcgaccggg ccgtcaccgg ggtcgagctc
     1081 gacccagagt cgcttcgctt ccagtgccac cgaagaaccg gcccgtgaac ccgaatccag
     1141 acccctccca cgctttccgg gggcgtctgg ccaccgattc ggtggctgat cgcccccgga
     1201 atcgcgggcg cggaggatct ggcgggcatc gcgaactccg accggcaggg gctcgatgtc
     1261 tgacccggcg aacatcaggg cagaaggcgt ggccagctcg ggcttgttcg cgatcgccca
     1321 ggcgaggaag tcccggggag ttccgtcgaa gccgatgcgg ccctcgtcca tggcgacaac
     1381 ccggtcggcg gcgggcaggc agcgctcgag ccggtgctcg gccagaacca ccgccacgcc
     1441 ccactcctcg ttcaggcgcc tgagcagcca gatcagctca tcgccggata ccgggtcgag
     1501 ctgcgaggtc ggttcatcga gcaggatcag cggcggccgg gtgaccaggg cgacggcaag
     1561 cgcgacccgc tggagctcac cgccggagag cgtggtggct tgttgtccta agcggatata
     1621 gcctaacccg acatcgaaaa gggtctgtaa gacagatttg attttaggga tattatcaaa
     1681 tatctctagg gcctcttcaa cggataagtt aagaacatcg gcgatattct ttcctttaaa
     1741 ctcgacctcc aaggtttgat cagtgaatct tttgccgtgg caaacctcac attccacgta
     1801 aacatccggc aagaaatgca tctcaatttt cttgatacca tctcccccgc aggtttcaca
     1861 gcgcccaccc ttgacgttaa aactaaaacg gcccggcttg tatccccgca tttttgattc
     1921 cggtaattga ctaaacaagt ctctgattcc tgaaaacatc cccgtgtatg ttgccgggtt
     1981 agaacgtggg gtacgcccta
//
LOCUS       DAOQMF010000373         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38818_length_1999_cov_3.406893, whole genome shotgun sequence.
ACCESSION   DAOQMF010000373 DAOQMF010000000
VERSION     DAOQMF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38818_length_1999_cov_3.406893"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..933)
                     /locus_tag="PLV77_06735"
     CDS             complement(19..933)
                     /locus_tag="PLV77_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNC15577.1"
                     /translation="MTSARGRIRVMIIATAVALISTLGPAGSATASPDASASIINGNP
                     ASIGDWPWQVAIASSTSKRPGQSPFYRTFCGGSIVAPTVILTASHCALDMYKGEVGDY
                     TVISGRTNLNNQAAGQEVFVTGIYFPRTSNGKILYKTRQAWDVSLLTLEEPLAGQPVK
                     LLGPDEHSILKPGRRLIETGWGSTITGRDVYPKTLKMGGTNIQPPSVCHGLLGSRYFK
                     PSLQVCLGDAKANQANCYGDSGGPAVVATSAGYRQVGVTSFGTADNCAGLIPNVDVLV
                     GGGPVRDWIQAGVQVLGGVDPVGSSVSH"
     gene            1028..>1999
                     /gene="prfB"
                     /locus_tag="PLV77_06740"
     CDS             1028..>1999
                     /gene="prfB"
                     /locus_tag="PLV77_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157591830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNC15578.1"
                     /translation="MGARLAAIDEHVALLADYLDPEGLEAEVAEFEQVMQQPGFWDDQ
                     DKAASVSADHARVQRKLEGFRALSSEAGDLAEMAEMAELDEEIAAELEEQMSSVEARL
                     EELEEARLFSGEYDHGDAIVSVHAGAGGTDSQDWAEILLRMYLRWAERRGFSVEMREA
                     SEGEEAGLKSATFLLKGENAYGLIAAERGVHRLVRISPFDSSSRRHTSFAQVDVAPVV
                     DEDVEIEIDDAEIRVDTFRASGAGGQHVNKTDSAVRITHEPTGVVVQCQNERSQTQNK
                     AVAMQMLKSKLVELEERKRAEAVNQERGEVKDVAWGSQIRSYVLQPYT"
BASE COUNT          370 a          615 c          671 g          343 t
ORIGIN      
        1 tatcgatatc ttttgatatc aatggcttac gctgctgccg accggatcga ctccaccgag
       61 tacctgaact cctgcctgga tccaatcgcg gaccgggccg ccgccgacca gcacgtcaac
      121 attcgggatc aggccggcgc agttgtccgc ggtgccgaaa ctggttaccc cgacctgccg
      181 gtagccggcc gaggttgcga cgaccgccgg tccgccgctg tcgccgtagc aattggcctg
      241 gtttgccttc gcatcaccga gacagacctg gagggagggc ttgaaatacc tggaaccgag
      301 caggccatgg cagaccgatg ggggctggat gttcgtgcca cccatcttca gcgtcttcgg
      361 gtagacgtcc ctgccggtga tcgtgctgcc ccatccggtt tcgatcaggc ggcgtcccgg
      421 tttgaggatc gagtgttcgt ccggtccgag cagcttgacc ggctggccgg caagcggctc
      481 ctcgagcgtc agcagggaaa cgtcccacgc ctgccgggtc ttgtagagga tcttgccgtt
      541 gctggtccgg gggaagtaga tcccggttac gaagacttcc tgcccggctg cctggttgtt
      601 gaggttggtc ctgcccgaga tgaccgtgta gtcgccaacc tcgcccttgt acatgtccag
      661 ggcacagtga ctggccgtga ggatcacggt cggtgcgact atcgaaccac cgcaaaaggt
      721 ccggtagaag ggggactggc ccggccgctt gctcgtcgag gaggcgatcg cgacctgcca
      781 cggccagtcg ccaatggaag ccggattgcc gttgatgatg ctggccgaag cgtccggcga
      841 agccgtcgct gatcccgccg gtccgagcgt ggagatcagg gccacggcgg tggcgatgat
      901 cattacccgg atgcgccctc tggctgaagt catgtttctc cctgttgaca ctctgcttgg
      961 ggtacatctt cgcaggcatc gggatgggta ggctccggtt gtggccgaag ccctgaacac
     1021 cgagtcgatc ggagcccgcc tggcggcgat cgacgaacac gtcgcactcc tcgccgatta
     1081 cctcgacccc gaagggctcg aagccgaagt ggcggagttc gagcaagtaa tgcagcagcc
     1141 gggattctgg gatgaccagg acaaggccgc gtcggtttca gccgaccacg cgagagtcca
     1201 gcggaagctt gagggtttcc gtgccctcag ctcggaagcg ggcgacctcg ccgagatggc
     1261 ggaaatggcc gaactcgacg aggaaatcgc ggccgaactc gaagagcaga tgagctcggt
     1321 cgaggcgagg ctcgaggaac tggaagaagc gaggctcttc agcggcgaat acgaccacgg
     1381 tgacgcgatc gtctcggttc acgccggggc cggcgggacc gactcccagg actgggccga
     1441 gatcctgctc cggatgtacc tgcgctgggc cgagcgacgc ggcttctcgg tcgagatgcg
     1501 ggaagcatcc gaaggcgaag aagccgggct gaaatcggcc accttcctgc tcaagggcga
     1561 aaacgcctac ggtctgatcg ccgcggaacg gggcgtccac cggctggtcc ggatctcgcc
     1621 cttcgattcc tcgtcccgcc ggcacacaag cttcgcccag gtggacgttg ccccggtcgt
     1681 cgacgaggac gtggagatcg agatcgacga cgccgagatc agggtggaca ccttccgggc
     1741 ctccggcgcc ggcggccagc acgtcaacaa gaccgactcg gcggtgcgga tcacccatga
     1801 gccgaccggg gtagtggtcc agtgccagaa cgagcgctca cagacccaga acaaggcggt
     1861 cgcgatgcag atgctcaagt cgaaactggt ggagctcgag gaacggaagc gggccgaggc
     1921 ggtcaatcag gaacgcggtg aggtcaagga tgtcgcctgg ggctcccaga tccgctctta
     1981 cgtgctccag ccctacacg
//
LOCUS       DAOQMF010000374         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38852_length_1998_cov_3.208441, whole genome shotgun sequence.
ACCESSION   DAOQMF010000374 DAOQMF010000000
VERSION     DAOQMF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38852_length_1998_cov_3.208441"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLV77_06745"
     CDS             <1..270
                     /locus_tag="PLV77_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15579.1"
                     /translation="KGYGWQTPVPSTPPTPPAPPAPKRSPASDEERMIILRMVEEGKI
                     SVEQAADYVENGTKPDPEVTKTGIVVATKDNIDDPEVSKFLYPAK"
     gene            356..1669
                     /locus_tag="PLV77_06750"
     CDS             356..1669
                     /locus_tag="PLV77_06750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC15580.1"
                     /translation="MIVGGGYVGLHTAIELARTAPGSRIIVLEREHCGFGASGRNGGQ
                     VHSWWNQIPTLSTICGREEAKLLADESVAAIERIGELSNRHPEVDFRQSGWLWTASTE
                     AQQGSWDACLAECDAQGVEPFAQLSGARLEEKTGTSVTRSGVFESTGGTVNPQGLVDA
                     LKQEATELGVEVFERTPVLEVSGHGPASVRTPNGDTAAGKVILTTNAWAARNRALQPY
                     LYVVGSDIVLTNPLHEISDEHLPEVGLAACDSQPRVLYWSLRGDRRLAFGRGGGRIAP
                     FNRIGRHSGGATRWRRDVITSMKRVYPKLGSAEPATAWSGAVDRTIDGFPIFGQTDRR
                     GPLYGVGWSGSGVVGSALGGRILASLACERDDFWSGNRLVTRRPIRLPREPLRTIGAH
                     IVRSAVRQSGKAEEQGTIPNPVTRKVAAMAPSLEADPESGPGAKQ"
     gene            1687..>1998
                     /locus_tag="PLV77_06755"
     CDS             1687..>1998
                     /locus_tag="PLV77_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15581.1"
                     /translation="MILSGENIPDYLDERPHLGDRVGTVTQVEMPDDGNINGVYIVQG
                     DRGSIVLKQGLPWVRIIETWELTAERTTREANLFEKWTPFAEGLLPEIFDFDADNHVL
                     AM"
BASE COUNT          403 a          624 c          659 g          312 t
ORIGIN      
        1 aagggctacg gctggcagac acccgtgccc tccacacctc ccaccccgcc agcgccgcct
       61 gcacccaagc gctcaccggc cagcgacgaa gaacgcatga tcattctgcg catggtcgag
      121 gaaggcaaga tctcggtcga gcaggctgcc gactacgtgg agaacgggac caagcccgat
      181 ccggaagtca ccaagaccgg catcgtcgtt gccaccaagg acaacatcga cgatccggaa
      241 gtctcgaagt tcctgtaccc ggccaagtag gtcagtgagg ccgggcaatc cagataccga
      301 atccgcgggc aacgaaagtt gcccgcggag cccggtccct tcaaaggccg actttgtgat
      361 cgtcggtggc ggctacgtcg gcctccacac ggcgatcgag ctggcgagga ctgcaccggg
      421 atcgcggatc atcgtgctcg aacgcgaaca ctgcggattc ggcgcttccg gccgtaacgg
      481 gggacaggtg cacagctggt ggaatcagat cccgaccctg tcgaccatct gcggacgcga
      541 ggaagcaaag ctcctcgccg atgaatcggt ggcggccatt gagcggatcg gggagctttc
      601 gaaccggcac cccgaggtcg acttcaggca gtcaggctgg ctctggacgg cctcaaccga
      661 agcacagcag ggaagctggg acgcatgtct cgccgagtgt gacgcgcagg gtgtcgaacc
      721 cttcgcgcaa ctgagcggcg caagacttga agaaaagacg ggaacgagcg tcacccggtc
      781 cggggtattc gaatctaccg gtgggacggt aaacccccag ggacttgttg acgcgctgaa
      841 gcaggaagcg accgaactcg gggtcgaggt cttcgaaagg acgccggtac tcgaggtttc
      901 cggccacgga ccggcgtcgg tcaggacccc gaacggggat accgcggcgg gcaaggtcat
      961 cctgaccacc aacgcgtggg ccgcccggaa ccgagcactt cagccgtacc tttacgtggt
     1021 tggctcggac atcgtgctga ccaatccgct tcacgagatt tccgacgaac accttcccga
     1081 ggtcggactg gcggcgtgtg attcacagcc gagagtgctc tactggtcgc ttcgcggaga
     1141 tcgccggctc gcattcggcc gtggcggcgg caggatcgct cccttcaatc gcatcggccg
     1201 gcactcgggc ggagcgacaa gatggcgtcg ggacgtaatc acttcgatga aacgcgtcta
     1261 tccgaaactc gggtcggccg agcccgccac agcctggtcc ggggcggtgg accggaccat
     1321 cgacgggttt ccgatctttg gccagaccga caggcggggt cccctctacg gggtcggctg
     1381 gagcggaagc ggtgtggtcg ggtcggccct cggcggcagg attctcgctt cgctcgcctg
     1441 cgagcgtgac gacttctgga gcggcaaccg gctcgtcacg aggcggccaa tccggctccc
     1501 gcgcgagccg ctgcgaacga tcggcgcgca tatcgtcagg tccgccgtac gccagagcgg
     1561 caaagccgaa gagcagggaa cgattccaaa cccggtcacc aggaaggtgg cagcgatggc
     1621 gccgtcgctg gaagcagatc ccgagtccgg gccgggagcg aaacagtgac ccgaccggcc
     1681 gacttcatga ttctgagcgg ggaaaacatt ccggattacc tggatgagcg accgcatctc
     1741 ggtgaccgtg tcggcacggt gacccaggtc gagatgcccg atgacggcaa catcaacggc
     1801 gtctacatcg tgcagggtga ccgtggttcg atcgttctga agcagggact gccctgggtc
     1861 agaatcatcg agacttggga gctgaccgcc gaacggacca cccgcgaagc gaacctgttc
     1921 gaaaagtgga ctcccttcgc cgagggactc ctgccggaga tcttcgattt cgacgcggac
     1981 aaccacgtgc tggcgatg
//
LOCUS       DAOQMF010000375         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38906_length_1996_cov_4.517259, whole genome shotgun sequence.
ACCESSION   DAOQMF010000375 DAOQMF010000000
VERSION     DAOQMF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38906_length_1996_cov_4.517259"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLV77_06760"
     CDS             complement(<1..208)
                     /locus_tag="PLV77_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC15582.1"
                     /translation="MAREAAPERRRQILDAAVRVFARQGFHSTRVADIADEADVAYGL
                     VYHYFSSKENVLNELFIQRWSLLIE"
     gene            complement(198..1040)
                     /locus_tag="PLV77_06765"
     CDS             complement(198..1040)
                     /locus_tag="PLV77_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNC15583.1"
                     /translation="MRAAVVQINSGADRSRNLEVAGELVVAAAADGAELVVLPEKWAL
                     LGDGAALTAGAESLDGEAITAARGWAADLGIHLLAGSFTMLPDPGGVPPSALPTNTSV
                     LISPEGQIVCSYDKLHMFDVEAGGVEYRESDHEQAGDGLAVADAGDLRIGLTICYDLR
                     FPELFRALLDRGATAFTVPSAFTSATGRDHWETLLRARAIENQAFVLAANQVGTAAPS
                     YDSWGHSMIVGPWGEVLAEVKEGEGFACAELDLERQAEVRRKLPAVNHRRPEIFSEGV
                     AHGS"
     gene            complement(1157..>1996)
                     /locus_tag="PLV77_06770"
     CDS             complement(1157..>1996)
                     /locus_tag="PLV77_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC15584.1"
                     /translation="NEQSARSLRTRNGLHQIETRLDGTDGDPGNKFSIDRVRQFTTNN
                     RNYGAQVLIPGMVNYCESHPTIDSVDVSAACTVLDQWDWTENLDSPGAYLGREVIGNL
                     LAVSGGPFADPFSLSDPVNTPSGLDTSKPGVPLALSAAVTYMNSKSIPLDASWRDYQY
                     VTKNGEQIPIPGGFGGQGMFNVTSAVRNSSTGVYDSVRHGSSFIITASLTGAKCPDVK
                     TILTYSQAATNELSPHYADQTKLYSNSGWVTDRFCVAQQKSSPGLKVTSLNGGAKAVK
                     KGW"
BASE COUNT          342 a          631 c          675 g          348 t
ORIGIN      
        1 cctcgatcag aagcgaccag cgctggatga agagctcgtt gaggacgttc tccttggagg
       61 agaagtagtg gtacaccagg ccgtaggcga catcggcctc atcggcgatg tcggccaccc
      121 gggtggagtg gaagccctga cgggcgaaga cgcggaccgc cgcgtcgagg atctggcgcc
      181 ggcgttccgg cgcggcctca cgagccatga gcgactcctt cactgaaaat ttccggccgg
      241 cgatggttta ccgccggcag cttgcggcgg acctccgcct gcctttcgag atcgagttcg
      301 gcacaggcga acccctcccc ctccttcact tcggccagca cctcgcccca gggaccgacg
      361 atcatcgaat gcccccagga gtcgtacgaa ggcgcggcgg taccaacctg gttggccgcc
      421 agaacgaatg cctggttctc gatcgcccgg gccctgagca gcgtctccca gtggtccctg
      481 ccggtggcgc tggtgaaggc cgacggcacc gtgaacgcgg tggcgccgcg atcgagcagg
      541 gcccggaaga gttcgggaaa gcgcaggtcg taacagatgg tcaggccgat tctgagatcg
      601 ccggcgtcgg ccaccgccag accatctccg gcctgctcgt ggtcggactc gcggtactcc
      661 actccgcccg cttcgacgtc gaacatgtgg agcttgtcgt aggagcacac gatctgccct
      721 tcgggggaga tcaggaccga ggtgttggtc ggcagagccg aagggggaac gccaccgggg
      781 tcggggagca tcgtgaagct gcccgccagc aggtggatgc cgagatccgc ggcccagcca
      841 cgggcggccg tgatcgcctc tccgtcgagc gactcggccc cggcggtgag agcagcgcca
      901 tccccgagca gtgcccattt ctctggcagc acgaccagct ctgcgccgtc agctgcggcg
      961 gcgacaacca gttcgccggc aacttcgagg ttgcgggagc ggtcggcacc tgagttgatc
     1021 tgtacgacgg cagctctcat gatcgggact tgattgacac gtcaatcaag aaggaaagta
     1081 taggtcgatt aagaaaccgg agcgcccggc ctcaaggccg ggcgctccga atacagcggg
     1141 tgtggcgggt gctcccctac cagcccttct tcacggcctt ggcaccaccg ttgaggctgg
     1201 tgaccttgag gccgggtgag ctcttctgct gggcgacgca gaagcgatcg gtgacccagc
     1261 cgctgttcga gtagagcttg gtctggtccg cgtaatgcgg ggacagctcg ttcgtggcgg
     1321 cctgcgagta ggtcaggatc gtcttcacgt ccggacactt cgcgccggtc agcgacgccg
     1381 tgatgatgaa gctcgagccg tggcgcacac tgtcgtacac gccggtggat gagttcctca
     1441 ccgcggaggt cacgttgaac atgccctgac cgccgaaacc gccggggatc gggatctgct
     1501 caccgttctt ggtcacgtac tggtagtcgc gccaggaagc gtcgagcggg atgctcttgc
     1561 tgttcatgta agtgaccgca gccgagaggg ccagcggcac gcccggcttg gacgtgtcga
     1621 gaccactcgg ggtattgacc gggtcgctca ggctgaacgg atccgcgaac ggaccgccgg
     1681 aaaccgcgag caggttgccg atcacctcgc gaccgaggta ggccccgggc gaatcgaggt
     1741 tctcggtcca gtcccactgg tcgagcaccg tgcaggctgc cgacacatcg accgagtcga
     1801 tcgtcgggtg gctctcgcag tagttgacca tgcccggaat cagcacctgg gcgccgtagt
     1861 tccggttgtt cgtggtgaac tgacgcacgc ggtcgatcga gaacttgttg cccgggtcac
     1921 cgtcggtgcc gtcgagcctg gtctcgatct gatgcaggcc gttccgggtc ctcagcgacc
     1981 gggccgactg ctcgtt
//
LOCUS       DAOQMF010000376         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_38947_length_1995_cov_3.250000, whole genome shotgun sequence.
ACCESSION   DAOQMF010000376 DAOQMF010000000
VERSION     DAOQMF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38947_length_1995_cov_3.250000"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLV77_06775"
     CDS             <1..539
                     /locus_tag="PLV77_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15585.1"
                     /translation="PSLPCRGEARGSCDDEDRVVLEAGPRAATLTALTTGGVVEVALT
                     GNATAPLTYRAKMQIKFPKKAVVGRSARFKVTLTNTGTGPIAGAALEYKARQGKSGKA
                     VVSKSVKVPALQSGRKTAMTVTVKLPKNRLSRGKGKGLLVNFELVHQGLTLAARRGTT
                     RLDFGQAKSRRPRDVSDT"
     gene            614..>1995
                     /locus_tag="PLV77_06780"
     CDS             614..>1995
                     /locus_tag="PLV77_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNC15586.1"
                     /translation="MKFRLMFGLFAAASLFALPNQAGAEISMNPMPGNPLTDTGQGGM
                     VRPVDLNDDGLDDLAIGSATAATGGVRIRIAGADGAWSPKPTLLAGSPVGDIVPADFD
                     QDGNEDLPVQISFPGLSANDQYRILSGHGDGTFAAPTSVAVPSGKVVISLPTGHNILP
                     TDIDGDGNLDLFIGLTRGQIAVARGHGDGSFDPLGVSASFPDTPTADSEGIGNVVAGD
                     YNADGRTDYAFTLLGNSAGTSTQANSGFYVMYGQGGASFSEVTRIGMADPQGPITSAF
                     SDDLNGDGSDDLIIAAVSPETTSTRFILYPGGNGGFATLPTTLFSLPVITPRVSGADF
                     NRDGNLDLAWVERADSGSPRANNLVLATGNGSGGFTRLSGAFDLNFGATQDGVSTASP
                     GDFNGDGSPDFAASFYSSDCAVNACGVSILMNRAVIGPATSSVDFGTVLQGTATAART
                     FTLTNTGSAPA"
BASE COUNT          366 a          688 c          618 g          323 t
ORIGIN      
        1 gaccgagcct tccttgtaga ggcgaagcgc gcggatcatg cgatgatgaa gaccgtgtcg
       61 tactcgaggc cggtccccgc gccgccaccc tgaccgccct gaccacgggt ggcgtggtcg
      121 aggtcgccct gaccggcaac gccaccgcgc ccctcaccta cagggcgaag atgcagatca
      181 agttcccgaa gaaggctgtg gtcggcaggt cggccagatt caaggtgact ctgaccaaca
      241 ccggcaccgg gccgatcgcc ggcgccgcac tcgagtacaa ggccaggcag ggcaagagcg
      301 gcaaggccgt ggtcagcaaa tccgtgaagg tccctgcctt gcagagcggc cgcaagaccg
      361 cgatgacggt cacagtcaag ctgccgaaaa accggctctc ccgcggcaag gggaagggac
      421 tgctggtcaa cttcgagctg gttcaccagg gtctgaccct ggccgcccga agaggcacga
      481 cccgtctcga tttcggtcag gccaagagcc gccggccgcg ggacgtctcc gacacctaga
      541 agcccgcgac cgcaaggtcc gtcacaatca ctcctgcttg ggaaagccaa ccagaaggtt
      601 gagggagaac accatgaagt ttcgtctgat gttcgggcta ttcgcggccg catcgctttt
      661 tgccttgccg aatcaagcgg gggccgagat ctcgatgaac ccgatgccgg ggaatcccct
      721 gaccgacacc gggcagggag gaatggtccg accggtcgac ctcaacgacg atggcctgga
      781 cgacctggcg attggatctg ccaccgccgc cacaggcggg gtccgcatcc ggattgccgg
      841 cgccgacggc gcctggagcc cgaaaccgac cctgctggcc ggctcaccgg tcggtgacat
      901 cgttccggct gattttgacc aagacggcaa tgaggacctt ccggttcaga tcagcttccc
      961 cggcctgtcg gccaatgatc agtaccggat cctttccggt cacggcgacg gcactttcgc
     1021 cgcgccgacc agcgttgccg tgccgtccgg caaggtcgtc atctccctgc ccacgggtca
     1081 caacattctc ccgaccgata tcgacggtga cggcaatctg gatctgttca tcggcctgac
     1141 tcgcggtcag atcgcggtcg cccgcggcca cggcgacggc agtttcgacc cgctcggcgt
     1201 ttcggcaagc ttcccggaca cccccacggc tgactcggag ggcatcggca acgtcgtcgc
     1261 cggggattac aacgcggacg gccggaccga ttacgcattc accctgctcg gcaactctgc
     1321 gggcacctcg acccaggcca attcgggctt ctacgtgatg tacggacagg gtggcgccag
     1381 tttcagcgaa gtgaccagga tcggcatggc cgatccgcag gggccgatca ccagtgcctt
     1441 ctcggatgac ctgaacggtg acggcagcga cgacctgatc atcgcggccg tgtcgcccga
     1501 aaccacctcg acccgtttca tcctctaccc cggcggcaac ggcggattcg ccaccctgcc
     1561 gacaacgctc ttctcactgc cggtcatcac accccgagtc tccggcgccg acttcaaccg
     1621 ggacgggaat ctggacctgg cctgggtcga gcgagccgac agcggcagcc cgcgagcaaa
     1681 caacctggtc ctcgccaccg gcaacggcag cggcgggttc acccggcttt ccggtgcctt
     1741 cgacctcaac ttcggtgcga cccaggacgg tgtttccacc gccagcccgg gcgacttcaa
     1801 cggtgacgga tcgcccgatt tcgcggccag cttctacagc tcggactgtg cggtgaacgc
     1861 atgcggagtt tcgatcctga tgaaccgggc ggtgatcggc ccggccacct cgtcggtcga
     1921 tttcggcacc gtgctgcagg gaacggcgac ggccgccaga accttcacgc tgaccaacac
     1981 cggttctgcg ccggc
//
LOCUS       DAOQMF010000377         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_39034_length_1993_cov_2.461816, whole genome shotgun sequence.
ACCESSION   DAOQMF010000377 DAOQMF010000000
VERSION     DAOQMF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39034_length_1993_cov_2.461816"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..604)
                     /locus_tag="PLV77_06785"
     CDS             complement(110..604)
                     /locus_tag="PLV77_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNC15587.1"
                     /translation="MAAPDRNEFRQTVGLLPTGVTVVSAFQASGPAGATASAVCSLSL
                     EPMMMLVCLDLGSRSLEAVREAGRFGISVLGESQRAAAEMFATKADQEEKWSSVVWRE
                     HLEVPIIEGSLARVVCDLAEVIPGGDHVIVTGEVLEVEAEDEPGEPLIYHGGLFRRLD
                     AQQD"
     gene            637..1083
                     /locus_tag="PLV77_06790"
     CDS             637..1083
                     /locus_tag="PLV77_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC15588.1"
                     /translation="MRAVAITTLAVAAALGLSACGFGEEGITVSKDSPDYEGAQLFAA
                     NCAGCHTLTPAGALGTGNRGTRNQGPNLDQRAVTYEDALHAIQNGGFSGAIMPQNIVV
                     GEEAEQVARFVAEYAGSDAGEAGAEAYSAEEDASSPQDEPIPTDGQ"
     gene            1099..>1993
                     /locus_tag="PLV77_06795"
     CDS             1099..>1993
                     /locus_tag="PLV77_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNC15589.1"
                     /translation="MLDLKLIRSDPERIKAALARRGAADQVDALLTLDERRRQLLPEI
                     EGARSERKQESEKIGALKKAGEDASAQMEAVRQLKARLEEMEAELEAVDGKLREVAAT
                     LPNLPDPTSPDGYTDEDAELIREVGEIPDFGFEPRDHVELAGDSIELEAAAKVSGARF
                     AYLKGDLVMLEFALVRWALEKLRAEGHEPVVPPILVREEALFGTGFLPGDRDQIYELE
                     KDELFLAGTSEVPLAGLHADQILEADQLPLRYAGFSSCFRREAGAAGRDTHGIFRVHQ
                     FDKVEMFSFVKPEDAAAEHERI"
BASE COUNT          372 a          680 c          647 g          294 t
ORIGIN      
        1 cacggcgagc ccgttgcccg ccagcgggga ttcggcgaag acgtcgagcc agaagaaggg
       61 gtggctgctc atcggccaag cctagccggg gcaaaccgga ccgggtgcgt caatcctgtt
      121 gtgcatcgag ccggcgaaag agcccgccgt ggtagatcag cggctccccc ggttcgtcct
      181 cggcctcgac ttcgagtacc tcgccggtca cgatcacgtg gtctccgccc ggaatcactt
      241 cggccaggtc gcagaccacc cgggccaggc ttccctcgat gatcggcacc tccagatgct
      301 cccgccagac gaccgacgac catttctctt cctggtccgc cttcgtcgcg aacatctcgg
      361 ccgcggcccg ttgcgactcg ccgaggaccg agatcccgaa acggccggcc tcgcgaaccg
      421 cctccagaga gcgggaaccg aggtcgaggc agaccagcat catcatcggc tcaagggaga
      481 gcgaacagac cgcggaagcg gtcgcgccgg ccggtcccga ggcctgaaat gcggagacca
      541 cggtgactcc ggtcggcaac aaacccaccg tctggcgaaa ctcgtttcgg tccggggctg
      601 ccatcgtttc gccgcagaca atatgatgcg ctcgctgtgc gtgccgtagc catcacaaca
      661 cttgccgtcg ccgccgccct cggcctgtcc gcatgcgggt ttggcgaaga aggaatcacc
      721 gtttcgaagg attccccgga ttacgaggga gcgcaactct tcgctgccaa ctgcgccggc
      781 tgccacaccc tgaccccggc cggggctctc ggaaccggca accgcgggac ccggaaccag
      841 ggtccgaacc tcgaccagcg ggccgtgacc tacgaagacg cccttcacgc aatccagaac
      901 ggtggattct ccggcgcgat catgccgcag aacatcgtgg tcggggaaga agccgagcaa
      961 gtcgcccggt tcgtcgccga atacgccggc tccgacgccg gtgaggcggg cgccgaggct
     1021 tactcggcag aggaagacgc ttcctccccg caggatgagc cgatcccgac cgacggccag
     1081 taaccggcgg cgggggccgt gctcgacctc aaactgatcc gctccgatcc cgagcggatc
     1141 aaggctgccc tggcccggcg cggcgccgcc gaccaggttg acgcgctgct cacgctcgac
     1201 gagcgccggc gacagctcct gcccgaaatc gaaggcgccc gcagcgagcg caaacaggaa
     1261 tccgagaaga tcggtgcgct gaagaaggcc ggcgaggacg cctccgcaca aatggaggcc
     1321 gtgcgccagc tgaaggccag gctcgaggag atggaagccg agctggaagc cgttgacggc
     1381 aagctccggg aggttgctgc gacgctgccc aacctgcccg acccgaccag ccccgacgga
     1441 tacaccgacg aagatgccga gctgatccgc gaggtcggcg agattccgga tttcggcttc
     1501 gagcccaggg accacgtgga acttgccggc gactcgatcg agctggaagc ggcggcaaaa
     1561 gtctccggtg ccaggttcgc ctacctcaag ggggatctgg tcatgctcga attcgcgctg
     1621 gtgcgctggg cgctggagaa acttcgagcc gaaggccacg agccggtggt gccgccgatc
     1681 ctggttcgcg aggaagccct gttcgggacc ggttttttgc ccggcgatcg cgatcagatc
     1741 tacgaactgg agaaggacga gctcttcctg gcgggcacct cggaagtgcc gctggccggc
     1801 cttcatgccg accagatcct cgaagccgat cagctgccgc ttcgctacgc cgggttctca
     1861 agctgcttcc gccgcgaagc gggcgccgca ggccgggaca cccacggcat cttccgggtc
     1921 catcagttcg acaaggtcga gatgttctct ttcgtcaagc ccgaagacgc ggcggccgaa
     1981 cacgagcgga tcc
//
LOCUS       DAOQMF010000378         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_39309_length_1985_cov_2.939896, whole genome shotgun sequence.
ACCESSION   DAOQMF010000378 DAOQMF010000000
VERSION     DAOQMF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39309_length_1985_cov_2.939896"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1598
                     /locus_tag="PLV77_06800"
     CDS             117..1598
                     /locus_tag="PLV77_06800"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNC15590.1"
                     /translation="MDGRGVVAAGHPLSAEAGARILREGGNAVDAAVAAALASFSLES
                     ALTGLGAGGFMIVGGPGIETAVIDFFVAAPGRGGHELTEELLPVPVHFDESTTQVFNA
                     GAASCGVPGTAAGLALALERYGSVPMAELTGPAAALAREGAPLNEQQAYVLRILEPIY
                     TATPEARAIYAPEGKILGQGDVFRYPDLGDALDRFGAEGAGALCSGEVPRLVEEYIVS
                     RGGTLSVADFEAYEPIVRAPVGARYRGREMLTNPPPSAGGILVAYSLALLERMEGSGP
                     EEIARAMAAANEARDDDFALSLGQEGFARRFLDDRLGSTTHLAAIDSDGLCASVTCSN
                     GTGSGMVVPGTGFHLNNMLGEEDLNPRGFHQSAPGERISSMMSPTLILEQGMVRAGLG
                     SAGSNRIRSAVLQTTLNLLAGVDPQAAVDAPRIHLEAGVLQAEPGVDEEALGRIEAAG
                     QEVFRWNQRNLFFGGVQIVTRDPETGRLDGAGDPRRGGAVAYG"
     gene            1591..>1985
                     /locus_tag="PLV77_06805"
     CDS             1591..>1985
                     /locus_tag="PLV77_06805"
                     /inference="COORDINATES: protein motif:HMM:NF028011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HNC15591.1"
                     /translation="MADYPDPDDYPSPDLRPFMAADVATRELPDFEAEGLLEGLEGDD
                     RAARIKLLTELYMQDGVELSTLKEEADANRLALLPSELVLGRARDKYTPAEIAELAGM
                     SVNDFQNLIAAVGLPRPKPDQRTFDDADLT"
BASE COUNT          302 a          664 c          692 g          327 t
ORIGIN      
        1 ctatctcgag gcgcctcgca ccggccgctc cacgcggctc gaccgcacgg cccggtcgtg
       61 gcgaatcgct gccggcagtc gggggtagcg acattcccga attcctctcc tgagctgtgg
      121 acgggcgcgg tgtcgttgcc gccggccacc cgctgtcggc cgaggccggc gccagaatcc
      181 tgcgtgaagg cggcaacgcg gtcgacgccg cggtagcggc tgccctcgcc tcgttctcgc
      241 tcgaaagtgc gttgaccggg ctcggcgccg gtggattcat gatcgtcggc ggtcccggaa
      301 tcgaaaccgc cgtgatcgat ttcttcgtcg ccgcgccggg ccggggcggt cacgaactga
      361 ccgaggagtt gcttccggtg ccggttcact tcgacgagtc gaccacccag gtcttcaatg
      421 ccggggctgc ttcctgcggg gttccgggca cggcggccgg tctcgctctc gccctggagc
      481 ggtacgggtc ggtgccgatg gcggagctga ccggccccgc agcggcgctt gcccgggaag
      541 gcgccccgct caatgagcaa caggcctatg tgctgagaat cctcgagccg atctacacgg
      601 cgaccccgga agcccgcgcg atctatgcgc ccgagggaaa gatcctcggc cagggggacg
      661 tcttccgcta tcccgacctc ggggacgcgc tcgaccgctt cggggccgag ggggcggggg
      721 cgctctgctc gggcgaggtt ccccggctgg tcgaggaata catcgtctcc cgcgggggca
      781 ccctctcggt ggctgatttc gaggcctacg agccgatcgt ccgggcaccg gtcggagcgc
      841 gataccgggg ccgcgaaatg ctgaccaatc cgccgccttc ggcgggcggg attctcgttg
      901 cctactcgct cgcgctgctc gagcggatgg aaggaagtgg cccggaggaa atcgccaggg
      961 cgatggctgc ggccaacgaa gcccgggacg atgacttcgc actcagcctt ggtcaggagg
     1021 gcttcgcccg caggttcctg gacgatcgcc tcggttcgac gactcatctg gcggcgatcg
     1081 attccgacgg tctctgcgct tctgttacct gttcgaacgg gaccgggtcc ggaatggtcg
     1141 tgcccggcac cgggtttcac ctcaacaaca tgctaggcga ggaagatctg aacccgaggg
     1201 gcttccacca gtccgctccc ggtgagagga tcagttcgat gatgtcaccc accctgatcc
     1261 tcgagcaagg catggtgcgg gccgggctcg gcagcgccgg gtccaaccgg atccgttcgg
     1321 cggtactgca gacgaccctg aacctgcttg ccggggttga tccccaggct gcggttgacg
     1381 cgccccggat ccacctcgag gcgggtgtgc tgcaggccga gccgggtgtc gatgaagagg
     1441 cgctcggccg gatcgaggcg gccgggcagg aagtgttccg ctggaatcag cgcaacctgt
     1501 ttttcggcgg cgttcagatc gtgacccgcg accccgaaac cggccggctt gacggcgccg
     1561 gtgacccgcg ccgcggcggg gcggtggcct atggctgatt acccggaccc ggacgactac
     1621 ccttcgcccg acctgcggcc cttcatggcg gccgacgtgg cgacccgcga gttgcccgat
     1681 ttcgaagcgg aggggctgct cgaaggactg gaaggtgacg accgggccgc ccggatcaag
     1741 ctgctgaccg agctctacat gcaggacggg gtggagctga gcaccctgaa ggaggaagcg
     1801 gacgccaacc gcctggccct gcttccctcg gagctggtcc tgggccgtgc ccgtgacaag
     1861 tacaccccgg ccgaaattgc cgaactggcc ggaatgtcgg tcaacgattt ccagaacctg
     1921 attgccgcgg tcggcctgcc gcgacccaag ccggaccagc gaaccttcga cgatgccgac
     1981 ctgac
//
LOCUS       DAOQMF010000379         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_39373_length_1983_cov_4.189315, whole genome shotgun sequence.
ACCESSION   DAOQMF010000379 DAOQMF010000000
VERSION     DAOQMF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39373_length_1983_cov_4.189315"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1694
                     /locus_tag="PLV77_06810"
     CDS             156..1694
                     /locus_tag="PLV77_06810"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNC15592.1"
                     /translation="MAVKAGKSTRFRSYSRQRWVDSGEGVVRLKTKVIRRRRGEKAPR
                     KDPGPRPLPRPQIPRNPWKPQPFGTYHGEFGRLEATRLLNRAGFGPRPGQAEQLAKMG
                     MKKAVLSLTRPQGQAKLIGPEPVDGDGLPLAPFDSWGHDHLWWLDRMVRTDQPFRERM
                     ALVFHDWFATSNSGVSQQRQMIDQYWTLRAGVNGTFDDLVVQITKDPAMLQWLSGGDN
                     TKWSPNENYARELMELFTLGADRGAYTEDDIREAARALTGWTYDWDDDLGAINFRFDP
                     EYHDDETKKIFSSKGNWDWRDVCRLCVTHELHASFFVDKLWSYFVPAAPSKDTRQALI
                     RLYLKNDREILPVVEAILMHPDFYRGPSMVKPPVVFNASLLRALKLPVNTDAWAWLGE
                     GAGQQLFLPPNVAGWDDERWLDTSTVRARWLMVTYALDSRHFEAWDDSYREKESVREA
                     VNRALAAYGWPALRSDHQNELVSMAGRISPYIQDDWQRRPYLAMRQNALLQLIASCPD
                     FQLS"
     gene            1691..>1983
                     /locus_tag="PLV77_06815"
     CDS             1691..>1983
                     /locus_tag="PLV77_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15593.1"
                     /translation="MNTGRCCSNYTRSNFLRSAAAKAGSGLPGIEPGMPVPAGTGLTR
                     RGFIAGAAGLALAVYAAGKTPLPLFEDGIAEAAQSDRILVSIFLDGGADTLSLL"
BASE COUNT          350 a          674 c          637 g          322 t
ORIGIN      
        1 acccacgccg acattgtgcc ggggtaccgc tccgcttgac ggccgatcgg gttgtggatg
       61 ggacgagacc gttcggttct ggtctggaaa cgggcgccgg cgatggggcg aagaaggttc
      121 cgcttgacgg ttggtgaaca atgtttgggc aggggatggc tgtaaaggca ggaaaaagca
      181 cgagattcag gagctacagc cgccagcgct gggtcgactc cggcgagggc gtcgtccgcc
      241 tgaagacgaa ggtgatccgt cgccgcaggg gcgagaaggc tccccgcaag gatcccggcc
      301 cccggccgct gccccggccg cagattcccc gcaacccctg gaagccgcag ccgttcggca
      361 cctaccacgg agagttcggt cggctcgaag ccacccgcct gctgaaccgg gcgggcttcg
      421 gcccgcggcc aggtcaggcg gagcagctgg cgaagatggg aatgaagaag gcggtccttt
      481 ccctgacccg gcctcagggc caggcgaagc tgatcgggcc cgaaccggtc gacggcgacg
      541 gcctgccgct tgcgccattc gattcctggg gtcacgatca cctctggtgg ctcgaccgca
      601 tggtccggac cgatcagccg ttccgggaac ggatggcact cgtcttccac gactggttcg
      661 caacctcgaa ctcgggggtc tcccagcaac gccagatgat cgaccagtac tggaccttgc
      721 gggccggcgt gaacgggacc ttcgatgacc tggttgttca gatcaccaag gatccggcga
      781 tgctccagtg gctgagtggc ggcgacaaca ccaagtggtc accgaacgag aactacgccc
      841 gagaactgat ggaactcttc accctcggtg ccgaccgggg cgcctacacc gaggacgaca
      901 tccgcgaggc ggcccgggcc ctgaccggct ggacctacga ctgggacgat gacctcggcg
      961 cgatcaattt ccgcttcgac cccgagtatc acgacgacga gaccaagaag atcttcagca
     1021 gcaaggggaa ctgggactgg cgggacgtct gccggctctg cgtcacccat gaactccacg
     1081 ccagtttctt cgtcgacaag ctctggagct acttcgttcc ggccgcgccc tcaaaggaca
     1141 cgcgacaggc cctgatccgg ctgtacctga agaacgaccg cgagatcctg ccggtggtcg
     1201 aggcgatcct catgcacccc gacttctacc gcggcccgtc gatggtcaaa ccgccggtcg
     1261 tcttcaatgc gagcctgctg cgggcgctga agctgccggt caacaccgac gcctgggcct
     1321 ggctcggcga aggcgcgggc cagcagcttt tcctgccgcc gaacgtggcc ggctgggacg
     1381 acgagcgctg gcttgacact tcgacggtca gggcccgctg gctgatggtc acctacgcgc
     1441 tcgactcccg ccacttcgag gcatgggacg actcgtaccg tgaaaaggaa tcggtccggg
     1501 aggccgtgaa tcgtgcactc gccgcctacg gatggccggc cctcaggtcg gaccaccaga
     1561 acgagctcgt ctcgatggcg ggccgaatct ccccttacat ccaggatgac tggcagcgtc
     1621 gcccctacct cgcgatgcgc cagaacgccc tgctccagtt gatcgcctcc tgcccggact
     1681 tccagctctc atgaacaccg gacgctgctg cagcaactac acccgctcga acttcctccg
     1741 ttcggccgcc gcaaaggcgg ggtccggcct ccccggtatt gagccgggca tgccggtacc
     1801 ggccgggacc ggactgaccc gacgcggctt cattgccggc gcggccggtc tggccctcgc
     1861 cgtctacgcg gccggcaaga ccccgctgcc gctctttgaa gacgggatcg ccgaggccgc
     1921 ccagtcagac cggatcctgg tttcgatctt cctcgacgga ggcgccgaca cgctttcact
     1981 gct
//
LOCUS       DAOQMF010000380         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_39494_length_1980_cov_3.690390, whole genome shotgun sequence.
ACCESSION   DAOQMF010000380 DAOQMF010000000
VERSION     DAOQMF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39494_length_1980_cov_3.690390"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..810
                     /gene="cofC"
                     /locus_tag="PLV77_06820"
     CDS             157..810
                     /gene="cofC"
                     /locus_tag="PLV77_06820"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNC15594.1"
                     /translation="MNSVAVITAKRFPAAKQRMAGGVPEERRLALVEAMLGDVLEAVT
                     GARQVNFTVVVTGEVAAADLAAGHGAEVIHDPEDAGHIGAAELGIERAIELGAECVIL
                     LPGDCPLLEAREIDSLLTGIPEKFVTVVPDRHGTGTNALVLRPPDAIKPAFGEGSCQR
                     HLRLARDAGLPHDTEEVAGLALDLDTPADIVALTTKIEMVRATGGNPAPRTAAVLGI"
     gene            811..1563
                     /gene="cofE"
                     /locus_tag="PLV77_06825"
     CDS             811..1563
                     /gene="cofE"
                     /locus_tag="PLV77_06825"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNC15595.1"
                     /translation="MDAAMIIPVEGLPEVTETFNIGKAIAERVQMTDGDIVAVSQKIV
                     SKSEGRVVRLDETDPGPDAVSLAARTGKDPRLVQHVLNESRNLIRVDEQRSVLITETH
                     HGYICANAGIDQSNTAGENQAILLPLDPDGSARRIRVELETASGVKAAVVVTDSFGRA
                     WRHGQVETAIGCAGIDPLDDWRGQEDQRGRELTATVIAVADQIAAASDLVRTKTDGVP
                     AVVLRGLDHFVTEDDGPGCAAQLREPGDDLFR"
     gene            1577..>1980
                     /locus_tag="PLV77_06830"
     CDS             1577..>1980
                     /locus_tag="PLV77_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNC15596.1"
                     /translation="MGRLIYGSMSITLDGYFADADGGIGWTVPEDEVHRLANQQVRGH
                     AAVIMGRGLYEMMVPYWYDLLGSGEGGEIEQEFAMVWDEIPKFVASRTLTAVHETCQL
                     IEGDAVDWIARKKAETDGDIEVSGIDLASQVEA"
BASE COUNT          357 a          638 c          685 g          300 t
ORIGIN      
        1 ctgcgcggcg acgcgtggtt cgacgacgcc ccggttgccg gacccgccgc cgaccgaggt
       61 ccgttccttc tccacggcga ccctgatgca cagcgccgag agccgggcaa cggtcgcccg
      121 ggccgtgatc aaggtcggcg agaccctggc cggatcttga actcggtcgc ggtaatcacg
      181 gcaaagagat ttcccgcggc caaacagcgc atggccggcg gagtgcccga agagaggcgc
      241 ctggccctgg tcgaggcgat gctcggcgac gtgctcgagg cagtgaccgg ggcaaggcag
      301 gtcaacttca cggtagtcgt caccggcgag gttgccgcgg cagacctggc ggccggccat
      361 ggcgccgagg tgatccacga cccggaggac gccggtcaca tcggtgccgc cgaactcggg
      421 atcgagcggg cgatcgaact cggagccgag tgcgtgatcc tcctgccggg cgattgtccg
      481 ctgcttgagg cccgtgaaat cgacagcctg ctgaccggca tcccggagaa attcgtcacg
      541 gttgttccag atcggcacgg aacgggtacg aacgcccttg tgctccgtcc tcctgatgcg
      601 atcaaaccgg ctttcggcga aggaagctgc cagagacacc tccgcctcgc ccgggatgcg
      661 gggctgccgc acgacacgga agaggttgcg ggactggccc tggatctcga tactccagct
      721 gacatcgttg ccctgaccac aaagatcgag atggttcgtg ccaccggggg aaatccggcc
      781 ccgcggaccg ccgccgtcct gggcatctag atggacgccg cgatgatcat cccggtcgag
      841 ggtctgccag aggtgaccga aaccttcaac atcggcaagg cgattgccga gcgggttcag
      901 atgaccgacg gcgacatcgt cgcggtttcg cagaagatcg tctccaagtc ggaaggtcgc
      961 gtcgtcaggt tggatgagac cgaccccgga ccggatgccg tctcgctggc cgcccggacc
     1021 ggcaaggatc cgaggctggt gcagcacgtg ctgaacgaga gccggaacct gatccgggtt
     1081 gacgagcagc ggagcgtcct gatcaccgag acccatcacg gctacatctg cgcaaacgcc
     1141 ggcatcgacc agtcgaatac ggccggcgag aaccaagcga tcctgctgcc gctggacccg
     1201 gacggttcgg cccggcggat ccgggttgag ctggaaaccg catcgggggt caaggcggcg
     1261 gtggtggtca ccgacagctt cggccgggcc tggcgtcacg gccaggtcga gaccgcgatc
     1321 ggctgcgccg gaatcgaccc gctggacgac tggcgcggcc aggaggacca gcggggccgg
     1381 gagctgaccg caaccgtaat cgcggtggcc gaccagatcg ccgccgcttc cgacctggtc
     1441 cggaccaaga ccgacggcgt gccagccgtg gtcctgagag ggctcgacca tttcgtcacg
     1501 gaagacgacg gcccgggctg cgcggcccag ctgcgcgaac ccggtgacga cctcttccgc
     1561 tagttaccct gagtccatgg gacgactcat ttacggatcg atgtcgatca cgctggatgg
     1621 ctactttgct gacgccgacg gcggtatcgg ctggaccgtg ccggaggatg aagtccaccg
     1681 gctcgccaac cagcaggttc gaggtcacgc ggccgtcatc atgggccgcg gcctctacga
     1741 gatgatggtc ccgtactggt acgacctcct ggggtccggc gagggcggtg aaatcgagca
     1801 ggagttcgcc atggtctggg acgagatacc gaagttcgtt gcctcgcgca ccctgaccgc
     1861 ggttcacgag acctgccagc tgatcgaggg cgacgcggtg gactggattg cccgcaagaa
     1921 ggccgagacc gacggcgaca tcgaggtgag cggcatcgac ctcgcctccc aggtcgaggc
//
LOCUS       DAOQMF010000381         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_39575_length_1978_cov_3.736349, whole genome shotgun sequence.
ACCESSION   DAOQMF010000381 DAOQMF010000000
VERSION     DAOQMF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39575_length_1978_cov_3.736349"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PLV77_06835"
     CDS             complement(<1..1036)
                     /locus_tag="PLV77_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC15597.1"
                     /translation="MNPVKSAAQAVANKAFEIRTLHKAGVVGGLGPAALPVAASALIR
                     GGTTPATAIRVAAVKNGDRVYLEDERGSLTFAEVHRRSNALAHAFAGLGIGPGDGLAV
                     MCRNHRGFVETNLAGWKLGANVILLNTMFSGPQLSDVIEREGAKAVVLDQEFEELLSG
                     VPGGIGRVCGFEDEPGTSGLPSLEDLIAGNDDGELPWPSEKGRNVILTSGTTGTPKGA
                     QRPAPSGLSVMVGMLDRIPHFSGETIVMAAPLFHSWGYLNSIIGTSVGARLVLQRRFN
                     PARTMELVDEYDADALVAVPVMLQRILELDAETSKHFKASKLGIVTLSGSALPGGLAT
                     AWMDRYGDNIY"
     gene            complement(1060..1521)
                     /gene="ispF"
                     /locus_tag="PLV77_06840"
     CDS             complement(1060..1521)
                     /gene="ispF"
                     /locus_tag="PLV77_06840"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNC15598.1"
                     /translation="MAISVGIGYDSHRFADDRRLILGGVEVPHEMGLSGHSDADVLTH
                     AIIDAVLGAAGLGDIGEVFPDTDPAWEGADSISLLSTVIGMLRGPITNVDATLICEQP
                     KLGPHRPAMVARLSEITGSRVSVKATTNEQMGWIGRGEGIACMAVAAVNLD"
     gene            complement(1562..>1978)
                     /locus_tag="PLV77_06845"
     CDS             complement(1562..>1978)
                     /locus_tag="PLV77_06845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC15599.1"
                     /translation="GRTGWVRGSRIRLLRSAGPFRIVIDRSQRRLRLYRHKRMVMKTK
                     VAVGTPDAPTPLGHFYLTAGFVPGDDFLGPWAFETSAYAAITDWPRGGIVGLHGTSET
                     WSVGLRASHGCLRVYNKVILALKKRVRPGTPLRIRA"
BASE COUNT          334 a          646 c          665 g          333 t
ORIGIN      
        1 tgtagatgtt gtcgccgtag cgatccatcc aggcggtcgc cagaccaccc ggcagggccg
       61 acccggaaag cgtcacgatc cccagcttgc tcgccttgaa gtgcttgctg gtctcggcgt
      121 ccagttcgag gatgcgctgg agcatgaccg gcaccgcgac cagcgcgtcg gcgtcgtatt
      181 cgtccaccag ctccatcgtc cgtgccgggt tgaagcgccg ctggaggacg agccgggccc
      241 cgaccgaagt gccgatgatc gagttgagat agccccagga atgaaacagg ggcgctgcca
      301 tcacgatcgt ctctccggag aagtgcggga tccggtcgag catgccgacc atcaccgaaa
      361 gaccgctcgg ggcgggccgc tgggcgcctt tgggagtgcc cgtggtgccg gaagtcagga
      421 tgacgttgcg acccttttcg ctcggccagg gcagctcgcc gtcgtcattc ccggcgatca
      481 ggtcctcgag gctgggcagg cccgaggtgc ccggttcatc ctcgaacccg cagacccggc
      541 cgatgcctcc cggaacgcca gagagcagct cctcgaactc ctggtccagg accacggctt
      601 tggcgccttc gcgttcgatc acgtcggaaa gttgcgggcc cgagaacatg gtgttgagca
      661 ggatcacgtt ggcaccgagc ttccagccgg ccaggttggt ctcgacgaag ccgcggtggt
      721 ttcggcacat cacggccaga ccgtcgccgg ggccgattcc gagcccggcg aaggcgtgag
      781 cgagggcgtt gcttcgccgg tgaacttcgg cgaaagtcag ggagccgcgt tcatcctcca
      841 gatagacccg gtccccgttc ttgacggcgg cgacgcgaat cgcggtcgcg ggagtcgtcc
      901 cgccccggat cagggccgag gcagcaaccg ggagcgcagc cgggccgagc ccgccgacca
      961 ccccggcctt gtgcagggtg cggatttcaa atgccttgtt ggcgacggcc tgggcggccg
     1021 acttgaccgg gttcatcgag gaatgactct acttgaggat cagtcgagat tgacggcggc
     1081 aacagccatg caggcgatcc cttcgccccg gccgatccac cccatctgct cattcgtggt
     1141 tgccttgacg cttacccggc tccccgttat ttcagacaac cttgcgacca tggccggacg
     1201 gtgggggccc agctttggct gctcgcagat caaggtggca tcgacgtttg tgatcggccc
     1261 ccgcagcatc ccgattaccg tgctgagcag gctgatcgag tcggcgcctt cccaggccgg
     1321 atcggtatca gggaagacct cgccgatgtc accgagcccg gcggcaccga ggacagcgtc
     1381 aatgatcgcg tgggtcagaa cgtcggcgtc cgagtgaccg ctcaggccca tctcgtgagg
     1441 aacctccacc ccgccgagga tcagcctgcg gtcatccgcg aagcggtggg agtcgtatcc
     1501 gatgccgacg ctgattgcca tcagcgggga tagtcgcagg atccgcagtc gacggggcag
     1561 ctcatgcgcg gatgcgcagc ggggttcccg gccggacccg cttcttcagg gcgaggatca
     1621 ccttgttgta gacccgcagg cagccgtggc tggcgcgaag gccgaccgac caggtttcac
     1681 tggtgccgtg cagcccgacg atcccgcctc gcggccagtc ggtaattgcc gcataggcgc
     1741 tggtctcgaa ggcccagggg ccgagaaagt catcaccggg gacgaaaccc gcggtcaggt
     1801 agaagtggcc gagcggggtc ggtgcgtcgg gggtgccaac cgcaaccttc gttttcatga
     1861 ccattcgttt gtggcggtag agccgcaggc gccgctgact gcggtcgatc acgatccgga
     1921 atggcccggc gctccgcagc agacgaatcc gcgaacccct gacccagccg gtgcgacc
//
LOCUS       DAOQMF010000382         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_39634_length_1977_cov_3.168574, whole genome shotgun sequence.
ACCESSION   DAOQMF010000382 DAOQMF010000000
VERSION     DAOQMF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39634_length_1977_cov_3.168574"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1371
                     /locus_tag="PLV77_06850"
     CDS             157..1371
                     /locus_tag="PLV77_06850"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNC15600.1"
                     /translation="MSTDALIFDAIRTPRGKGKSSGSLYSTKPVDLVVGLMHETLIRN
                     EKLDPNTIDDVVLGCVSPVGDQGADIAKTAAIKAGLPDTVAGVQLNRFCASGLEAVNI
                     AAQKVASGWEDLVLAGGVESMSRVPMGSDGGAWAMDPETNYDTSFVPQGIGADLIATI
                     EGFTRDDVDEFAVRSQERAATAQKEGRFANSVIPVKDFNDMVVLDHDEFIREGTTTES
                     LAKLNPSFAGMGEMGGFDAVALQKYHWVEQINHVHHAGNSSGIVDGASLVAIGSEKAG
                     ERNGMTPRARIVATAVSGADPTIMLTAPGPASKKALEKAGLTADDLDLIELNEAFAAV
                     ALRLVRDLDVDMEKVNVNGGAIAMGHPLGATGAMILGTIVDELERTGGRYGLCTLCVG
                     GGMGIATIVERV"
     gene            1400..>1977
                     /locus_tag="PLV77_06855"
     CDS             1400..>1977
                     /locus_tag="PLV77_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNC15601.1"
                     /translation="MSETKTIQWEKGDDGIVILTIDDPNQGANTMNRDYIESMGQIVD
                     RLDAEKESVTGVIITSGKKTFFAGGDLNDLLAVTKEQSVEFAAGIREVKSQLRRLETL
                     GVPVVAAVAGSALGGGLEITLATHHRIVVDDPKIQLGTPEVQLGLLPGGGGVVRTVRM
                     LGLADALMQVLIQGKPLRPAKAREIGLIDELV"
BASE COUNT          384 a          658 c          634 g          301 t
ORIGIN      
        1 ccgcgataac acgggcggtc tggtggtcgc caatccgatc taccgggaag tgcttcgcag
       61 catggaggcg aatctggcta tttgacataa ttttggggcc cttcctgtgt cagccagcac
      121 agaaaaatca cttctcttcc gaaacggagc ctcccgatga gcaccgacgc actgatcttc
      181 gacgccatcc gcacaccgcg cggcaaaggc aagagcagcg gctcgcttta ctcgaccaag
      241 ccggtcgacc tcgtggtcgg actgatgcac gagaccctga tccgcaacga aaagctcgat
      301 cccaacacca tcgacgacgt cgtgctcggc tgcgtttcac cggtcggtga ccagggcgcc
      361 gacatcgcca agaccgcggc gatcaaggcc ggcctgccgg acacggttgc cggagtccag
      421 ctcaaccgct tctgcgcctc cggcctcgaa gcggtgaaca tcgccgccca gaaggtcgcc
      481 tcgggctggg aggacctggt cctcgccggt ggcgtcgaat cgatgtcgcg cgtaccgatg
      541 ggctccgatg gcggtgcctg ggcgatggac cccgagacca attacgacac cagtttcgtc
      601 ccgcagggga tcggggccga cctgatcgcg acgatcgaag gtttcacccg ggatgacgtc
      661 gacgagttcg ccgtccgctc acaggaacgg gccgcgacag cccagaagga aggccgtttc
      721 gccaactccg tgattccggt caaggacttc aacgacatgg tcgtgctcga ccatgacgag
      781 ttcatccgcg agggcaccac gaccgagtcg ctggccaagc tgaatccctc tttcgccggg
      841 atgggcgaga tgggcggatt cgacgccgtc gcgcttcaga aataccactg ggtcgagcag
      901 atcaaccacg tccaccacgc cggcaactct tccggaatcg tcgacggcgc ctcgctcgtc
      961 gcgatcggca gcgagaaggc cggcgagcga aacggcatga ccccgcgggc ccgcatcgtc
     1021 gccaccgcgg tttccggagc cgacccgacg atcatgctca ccgcgcccgg cccggccagc
     1081 aagaaggcgc tcgagaaggc gggcctgacc gccgacgacc tcgacctgat cgagctcaac
     1141 gaggccttcg ccgcggtggc gctgcgactg gtccgcgacc tcgacgttga catggagaag
     1201 gtcaacgtga acggcggcgc gatcgccatg ggccacccgc tcggcgcgac cggggcgatg
     1261 atcctcggca cgatcgtcga cgagctcgag cgcaccggcg gccgttacgg gctctgcacc
     1321 ctctgcgtcg gcggcggcat gggaatcgcg accatcgtcg agcgcgtctg atccagggaa
     1381 agaccaggag aacctgaaca tgagtgaaac caagaccatc cagtgggaaa agggcgacga
     1441 cggaatcgtc atcctgacca tcgacgatcc caaccagggc gcgaacacga tgaatcgcga
     1501 ctacatcgag tcgatgggtc agatcgtcga ccgcctcgat gccgagaagg aaagcgtcac
     1561 cggcgtgatc atcacctcgg gcaaaaagac cttcttcgcc ggcggtgacc tgaacgacct
     1621 gctcgccgtg accaaagagc agtcggtcga gttcgcggca ggcatccgcg aggtcaaatc
     1681 gcagctgcgc cgcctcgaga cgctcggcgt cccggtcgtg gccgcggtgg ccggttcggc
     1741 gctcggcggc gggctcgaga taacgctcgc cacccatcac cggatcgtgg tcgacgaccc
     1801 gaagatccag ctcggtaccc cggaggtcca gctcggcctg ctgcccggcg gcggcggagt
     1861 ggtccgcacg gtacggatgc tcggtctggc cgatgccctg atgcaggtgc tgatccaggg
     1921 caagccgctc cgtccggcca aggccaggga gatcgggctg atcgacgagc tggtcca
//
LOCUS       DAOQMF010000383         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_39638_length_1977_cov_2.978148, whole genome shotgun sequence.
ACCESSION   DAOQMF010000383 DAOQMF010000000
VERSION     DAOQMF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39638_length_1977_cov_2.978148"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLV77_06860"
     CDS             complement(<1..747)
                     /locus_tag="PLV77_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNC15602.1"
                     /translation="MPYPVGTTTNDAGHLEIGGCDVIELAREFGTPSYLFATAEMRER
                     ARAYQRAFAAHTDHFEVLFASKALPVTAAYRLFAEEGLSVDVASGGELTMALTAGFDP
                     ARIYMHGNNKTDAELVLAGNSGVGHLIVDSFDEIDRCERVLKRKQDVLIRVTPGIKPS
                     THSFIQTGQLDSKFGFGLEDGTAAKAITRVLASDTLNLVGLHFHIGSQIFEIEPFRLA
                     VEALGELRGDWCSILDLGGGLGISYDEADSP"
     gene            803..>1977
                     /locus_tag="PLV77_06865"
     CDS             803..>1977
                     /locus_tag="PLV77_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15603.1"
                     /translation="MASSARRPINAPPGSPQGTGVPWLGLSILLVLLLILAAFVAYFF
                     FDVGKDEGGDSSSGVTELETGATGGQGAIVGHSNGPLGFPSAATRNTTRISGSDPVDI
                     SMAAALAAYPTSGPGSPPAAVTIIDANDWQSGVAAASLSALPIGSPILLAPSGTLSAT
                     GMDTLAQLDPLGSPDTGENKVFTVGKVAPPSGYDVERLDGNSPAEIAAAVADERAALT
                     DAPPNAFVVVSSEAPDYATPAATWAARSGDVVLYTGKDEVPKATLDVIKDKANKDVPI
                     FVLGPSDVVSSKALKQLDKAGSTVERVGGDDPESAAVELVRFSSGLFGWSLNEPGHGY
                     VLARTDRPMDAIAVTALSTGGTWAALLLTDSSEKLPQVVEDYLLDVKPGYETDPTKA"
BASE COUNT          349 a          643 c          638 g          347 t
ORIGIN      
        1 gggtgaatcg gcctcgtcgt aagagatccc gaggccgccg ccgaggtcga ggatcgagca
       61 ccagtcgccg cgcaactcgc cgagtgcctc gaccgccagg cggaagggct cgatctcgaa
      121 gatctgggag ccgatgtgaa aatgaaggcc gaccagattc agggtgtccg aggcaagcac
      181 ccgggtgatc gctttggcgg ctgtaccgtc ctccagcccg aagccgaact tcgagtcaag
      241 ctggccggtc tggatgaagg agtgggttga cggtttgatg cccggggtga cccggatcag
      301 cacgtcctgc ttgcgcttca gcacccgctc gcatcgatcg atttcgtcaa acgagtcgac
      361 gatcaggtgg ccgacccccg agttgccggc gagcaccagc tcggcgtcgg tcttgttgtt
      421 gccgtgcatg tagatcctgg ccgggtcgaa accggcggta agggccatgg tcaactcgcc
      481 acccgaggcg acatcgaccg aaagtccttc ttcggcgaag agccggtagg cggccgtgac
      541 cggcagggcc ttgctcgcga aaagcacttc gaagtgatcg gtatgtgcgg cgaaggcacg
      601 ctgataggcc cgggcccgtt cccgcatttc ggccgtggcg aagaggtagg acggggtgcc
      661 gaactcgcgg gcgagttcga ttacgtcgca gcctccaatc tcgaggtggc cggcgtcatt
      721 ggtggttgtc ccaaccgggt atggcatcgc cgccattctt acgatttccg gtgaacgaca
      781 ccgtttggat acggttcacg ccgtggcaag ctccgcaaga cggccgatca acgcaccgcc
      841 agggagtccg cagggcaccg gggtgccctg gctcggcctc tcgatcctgc tcgtcctgct
      901 gctgattctg gccgccttcg tcgcctattt cttctttgac gtgggcaagg acgaaggggg
      961 cgattcctcc tcaggcgtga ccgagctgga aaccggcgca accgggggcc agggcgcgat
     1021 cgtcggtcac tcgaacggcc cgctcggttt cccctcggcg gccacccgca acacgaccag
     1081 gatcagcggc agcgatccgg ttgacatttc gatggccgcc gcgctggccg cctaccccac
     1141 ttcaggaccc ggttcgccac cggcggcggt cacgatcatc gacgcgaacg actggcagtc
     1201 gggagtcgcg gcagcttcgc tcagtgcgct gccgattggc tcgccgatcc tgctcgcccc
     1261 gtcgggcacg ctttcagcga ccggcatgga cacgctggcc cagctcgatc cgctcggttc
     1321 gcccgatacc ggcgagaaca aggttttcac ggtaggaaag gtcgccccgc cttccggcta
     1381 tgacgttgaa cggctggacg gaaacagccc ggccgagatt gccgctgcag tggcagacga
     1441 gcgcgccgcc ctgaccgatg caccacctaa cgccttcgtg gtcgtctcct ccgaggctcc
     1501 cgattacgcc actccggccg cgacctgggc cgcccgatcc ggagacgtcg tcctctacac
     1561 cggaaaggat gaggtgccga aggccaccct cgacgtgatc aaggacaagg ccaacaagga
     1621 tgtcccgatc ttcgtgctcg gaccgtcgga tgtggtctca agcaaggccc tgaaacaact
     1681 cgacaaggcc ggctcaacgg tcgagcgggt cggcggcgat gaccctgaat ccgccgcggt
     1741 cgagctggtc cgcttctcga gcggcctctt cggctggagt ctgaacgagc ccggacacgg
     1801 ctatgtcctg gcccggacag atcgcccgat ggatgcgatc gctgtgaccg cactctctac
     1861 aggtggtacc tgggcggctc tgctcctgac cgattccagc gagaagctgc cgcaagtggt
     1921 cgaggactat ctccttgatg tcaagcccgg ctatgagacc gacccgacca aagcgat
//
LOCUS       DAOQMF010000384         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_39671_length_1976_cov_3.423738, whole genome shotgun sequence.
ACCESSION   DAOQMF010000384 DAOQMF010000000
VERSION     DAOQMF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39671_length_1976_cov_3.423738"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLV77_06870"
     CDS             complement(<1..355)
                     /locus_tag="PLV77_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNC15604.1"
                     /translation="MGRTSPSVEELKEGIARGDRGALSRAITLAESRRPDDQEHAQEL
                     IADLLPETGEAIRVGITGVPGAGKSTAIEALGLELIRHGHKVAVLTIDPTSARTGGSI
                     LGDKTRMHKLSVEEDV"
     gene            complement(355..>1976)
                     /gene="scpA"
                     /locus_tag="PLV77_06875"
     CDS             complement(355..>1976)
                     /gene="scpA"
                     /locus_tag="PLV77_06875"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNC15605.1"
                     /translation="GTIQNDILKEFMVRNTYIYPPKPSMRIVSDIIGYTSQEMPRFNS
                     ISISGYHMQEAGASADLELGYTLSDGVEYVKAALESGLDVDKFAPRLSFFWAIGMNFF
                     MEVAKMRAGRLLWANLMRQFNPQNPKSLSLRTHCQTSGWSLAAQDAYNNVVRTSVEAM
                     AATQGHTQSLHTNALDEALGLPTDFSARIARQTQLFLQQESGTTRVIDPWAGSYYVEY
                     LTNELARKALAHMDEIDEYGGMTEAIAAGIPKMRIEESAARTQARIDSGKQTVVGVNS
                     YRPEQDTWVEVLKVDGDEVRRAQIAKLERLRGERNEDDVRQALEHLTKAADSGEGNLL
                     DLGVKAARVYATAGEISEALEKVYGRHSADIKVIGGVYQGEVGVDTESFADTKRLVER
                     FEEVEGRRPRILVAKMGQDGHDRGQKVIATAFADLGFDVDIGPLFQTPEEVARQAIEA
                     DVHVVGVSTLAAGHLTLVPALRKALDELDRQDIAIVAGGVIPPADFDELKELGADLIF
                     PPGTVIGEAATELLLLLGERLDLDIQTGSPS"
BASE COUNT          291 a          645 c          667 g          373 t
ORIGIN      
        1 agacatcctc ctcgacgctc agcttgtgca tgcgggtctt gtcaccgagg atcgaaccgc
       61 cggtgcgggc cgaggtcgga tcgatcgtga gcaccgcgac cttgtgaccg tggcgaatca
      121 gctcaagacc gagcgcctcg atcgcggtcg acttgccggc cccgggcacg cccgtgatcc
      181 cgacccggat cgcctcaccg gtttccggca gcaggtcggc gatcagctcc tgggcgtgct
      241 cctggtcatc gggccggcgg ctttcagcca gggtgatcgc ccttgagagc gccccgcggt
      301 caccgcgggc gatgccctcc ttcagctcct cgaccgaagg gctggttctg cccattacga
      361 cggggagccg gtctggatgt ccaggtcgag cctctcgccg agcagcagca gcagctcggt
      421 cgctgcttcg ccgatcacgg tgccgggcgg gaagatcagg tcggcgccga gttccttcag
      481 ctcgtcgaaa tcggccggag ggatcacgcc gccggcgacg atcgcgatgt cctgccggtc
      541 cagctcgtcg agcgccttgc gcagggccgg gaccagggtg aggtggccgg cggcaagggt
      601 gctgaccccg accacgtgga cgtccgcctc gatcgcctgg cgggcaacct cttccggagt
      661 ctggaagagc gggccgatgt caacgtcgaa gccgaggtcc gcgaaggcgg tggcgatcac
      721 cttctggccc cggtcgtgac cgtcctgccc catcttggcg accaggatgc gcggccgtcg
      781 cccttcgact tcctcgaaac gctcgaccag tcgcttggtg tccgcgaagg actcggtatc
      841 cactccgact tctccttggt aaaccccgcc gatcaccttg atgtcggcgg aatggcggcc
      901 atagaccttt tccaaagctt cgctgatctc accggcggtg gcgtagaccc gggcggcctt
      961 gaccccgaga tcgagcagat tgccttcacc gctgtccgcg gctttggtca gatgctcgag
     1021 cgcctggcgc acgtcgtcct cgttgcgttc gccccgcagc cgttccagct tcgcaatctg
     1081 ggcacgccgg acttcgtcgc cgtcgacctt cagcacctcg acccaggtgt cctgctcggg
     1141 ccggtaggag ttgaccccga ccaccgtctg cttgccggag tcgatccggg cctgggtgcg
     1201 ggcggccgat tcctcgatcc gcatcttcgg gataccggcg gcgatcgcct cggtcatgcc
     1261 gccgtactcg tcgatctcgt ccatgtgggc cagcgccttg cgggccagct cgttggtcag
     1321 gtactcgacg tagtaggagc cggcccaggg gtcgatcacc cgggtggtgc cggattcctg
     1381 ctgcaggaag agctgggtct ggcgggcgat ccgggccgag aagtcggtcg gcaggcccag
     1441 cgcctcgtcg agcgcattgg tgtggagcga ctgggtgtga ccctgggtcg cggccatcgc
     1501 ctcgacgctg gtccggacca cgttgttgta cgcgtcctgg gcggcgaggc tccagccgga
     1561 ggtctggcag tgggtcctga gtgaaagtga cttggggttc tgcgggttga actggcgcat
     1621 caggttggcc cagagcaggc gcccggcccg catctttgcc acttccatga agaagttcat
     1681 cccgatcgcc cagaagaagg agagccgcgg cgcgaacttg tccacgtcga gacccgattc
     1741 gagggccgcc ttcacgtact cgaccccgtc cgaaagcgtg tagcccagct cgaggtcggc
     1801 gctggccccg gcttcctgca tgtggtaacc ggagatcgaa atcgagttga agcggggcat
     1861 ctcctgcgag gtgtatccga tgatgtccga gacgatccgc atcgagggct tgggcgggta
     1921 gatgtaggtg ttccgcacca tgaactcttt gaggatgtcg ttctggatgg tgccgg
//
LOCUS       DAOQMF010000385         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_40065_length_1966_cov_3.491889, whole genome shotgun sequence.
ACCESSION   DAOQMF010000385 DAOQMF010000000
VERSION     DAOQMF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40065_length_1966_cov_3.491889"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..376
                     /locus_tag="PLV77_06880"
     CDS             95..376
                     /locus_tag="PLV77_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15606.1"
                     /translation="METHSGQIVLRQNRKPLVRVSQNKDAATVVLTRCRKVYVKSLRI
                     HHGLIGEILIGAGIAFHKRSVTAVGALLVAHDRRDFPFPLTDPNGWGPK"
     gene            373..669
                     /locus_tag="PLV77_06885"
     CDS             373..669
                     /locus_tag="PLV77_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15607.1"
                     /translation="MRFLALTLIALVLARSVLDPEAGITLLQGLLLMALGVVAVNVIF
                     PRRGMSIWPLVVMHLLGRRNRRKEYRHAKAHPTDLRQGSRAARRTHHESGRKAA"
     gene            1341..1703
                     /locus_tag="PLV77_06890"
     CDS             1341..1703
                     /locus_tag="PLV77_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15608.1"
                     /translation="MENTTPYSAKLQVKRVHIADVTIQGKTERLTWIRTGGLSVNILI
                     TETVVGNKLTAPRVIADFTVDETEEEVKAMCRDYVRRQNIPHLDQLPMRRLTVDDQRP
                     DEEIEFDLAKEPLFGAPR"
     gene            1700..>1966
                     /locus_tag="PLV77_06895"
     CDS             1700..>1966
                     /locus_tag="PLV77_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15609.1"
                     /translation="MSPVAEQARTQTVTTVRLEDGRRITEHLTPDRQAKLYGLVVHGD
                     QLGMVDAARAERPEGGKRLKFPHRPEDQGDLWLPADNHDALAKLV"
BASE COUNT          430 a          622 c          585 g          329 t
ORIGIN      
        1 gaggaaaccc gggctgtccg ggcgatcacc gaccgcaaac acagcaactg accctcgccg
       61 cccaagtggc ggccacgtag aaaggagacc cgaaatggaa acccattccg ggcaaatcgt
      121 cctgcgccaa aacagaaagc cactggtgcg ggtaagccaa aacaaggatg cggcaaccgt
      181 cgttttgacc cgttgccgga aggtgtacgt gaagtccctg cggatccatc acggactgat
      241 cggggaaatc ctgatcgggg ccgggatcgc attccacaaa aggtctgtga ccgcggtcgg
      301 ggcgcttctg gtggcccatg accggcgtga cttcccgttt ccgctcactg acccgaacgg
      361 ttgggggccg aaatgagatt tctcgcactc accctcatcg ccctggtcct cgcaaggtcg
      421 gttctcgatc ccgaggccgg aatcacgctt cttcaaggcc tgctcctgat ggctttggga
      481 gtggtcgcgg tgaacgtcat ttttccgcgc cgcggcatgt cgatctggcc tttggtcgtg
      541 atgcacctgc tcggccgccg caacagaaga aaggagtatc gccatgcgaa agcgcatccg
      601 acggatcttc ggcagggcag ccgtgctgct cgccgcacac accatgaaag tggccgaaag
      661 gctgcttgac cgtcaacgac cgccggcaca ttcgtctgtg aaaaagccgg atccgaactg
      721 tcccggctgc tccagctcgg cggtcgccgg tctgttctgg ccggtcgaga tcaacggaga
      781 cagatcccgc gactggattg aacgctgcga cgagtgccga cgtttcgaca acgacattca
      841 ggctgcagcg gaactgatcc ggctcggcct gacggacgag ctcggtatcg ggaaaccggc
      901 cggatcaacg atcgacacgc cgttcgcaga acctttgggc cgcaggaaaa agtcccgaag
      961 gtagggcgtg tctggccagc tcaagccgga accgaagtcc cggtaccgac gcttatcggc
     1021 agccggccag acactacgaa aggaaatgtc aaatatttga cttttccgct tgcgaggtta
     1081 gcaatgacct tgctcaatag ggccgatcgg caacaccgat acgcccttcc gtgatccagg
     1141 gggggtgggt tttcgggtct ctgcggagac ctaccccata tgggggccat ctccaagacc
     1201 caccactccc ggatccaacc ggccccgaca cggggcctcc tatccgggtg cgctgagaac
     1261 ggggttatcc ccgttcaggc gcaccaaatg cctgtgcgga gcggctacgt gccgtttcgc
     1321 aaaaagcaag gagattcatg atggagaaca cgacgccgta ctcggcgaaa ctccaagtca
     1381 agcgcgtcca catcgcggac gtcaccatcc agggcaagac cgaaaggctc acctggattc
     1441 gcaccggggg actctcggtg aacattctga tcaccgaaac ggtggtcggc aacaagctca
     1501 ccgcgccaag ggtgattgcc gacttcaccg ttgacgagac cgaagaagaa gtgaaggcga
     1561 tgtgccgcga ctacgttcgc cggcaaaaca ttcctcacct cgaccagctg ccgatgcgtc
     1621 gactcaccgt cgacgaccag cggcccgacg aggaaatcga attcgatctc gccaaggaac
     1681 cgctgttcgg cgccccgaga tgagcccggt cgccgaacaa gcccgcacgc aaaccgtcac
     1741 aacggtgcgg ctcgaggacg gccgcaggat caccgagcat ctcacccccg accggcaggc
     1801 caagctttac ggcctggtcg ttcatggcga tcagctcggc atggtcgatg cggcccgggc
     1861 cgaacggcca gaaggaggca aacgcctcaa gttcccgcat cgtccggaag accaaggtga
     1921 cctctggctg ccggccgaca atcacgatgc gctcgcgaaa ctcgtc
//
LOCUS       DAOQMF010000386         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_40157_length_1964_cov_3.568360, whole genome shotgun sequence.
ACCESSION   DAOQMF010000386 DAOQMF010000000
VERSION     DAOQMF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40157_length_1964_cov_3.568360"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /gene="rnpA"
                     /locus_tag="PLV77_06900"
     CDS             complement(<1..331)
                     /gene="rnpA"
                     /locus_tag="PLV77_06900"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNC15610.1"
                     /translation="MSANGAGSPRRRRLSRSGDFKRAYREGSSKATRYLVLYRFDRSA
                     DDESEIRLGVSVSKKLGDAVTRNRVKRVLKEAFWSQVDRDEADQDFVLVARPGVGEVI
                     DTDGLAGA"
     gene            complement(334..468)
                     /gene="rpmH"
                     /locus_tag="PLV77_06905"
     CDS             complement(334..468)
                     /gene="rpmH"
                     /locus_tag="PLV77_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNC15611.1"
                     /translation="MKRTYQPKKRKRAKTHGFRARMSSKGGREVIRRRRRKGRKRLAV
                     "
     gene            761..>1964
                     /gene="dnaA"
                     /locus_tag="PLV77_06910"
     CDS             761..>1964
                     /gene="dnaA"
                     /locus_tag="PLV77_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNC15612.1"
                     /translation="MKEWETIQPTLKRRLFDSAWRMWIEPVTPVAVRGTTLYLQAPDP
                     IRTWVERRYIEVITETLVDSSSHLTAVSFENPPEPERVPAPTRPAGPNPTHDFDRFVI
                     GPGNHLAHAAALAVAEAPSESYNPLFLHGPPGLGKTHLLGAIANYLALHVPELNVLYT
                     NAESFTAEFVTALRDQGTDEFKRRYRNLDVLLIDDIQFIAGKPHTEEEFFHTFNALHD
                     AGSQIVLSADRSPGEISTLADRLSDRFEWGLTVSIEAPNLATRLTVLRHLVTEAGLEV
                     ADQDALTLLARRVQSNLRQLRGALTRTIAESSLNSAPITSELITQVFPATESAPAIQI
                     TPETIRSTVAAHYRTDVETLQSRRRDRNTATARHVAIYLTREITSLSLPQIGGLYGDR
                     DHTTVLNSI"
BASE COUNT          350 a          714 c          553 g          347 t
ORIGIN      
        1 cggctccggc aaggccgtcc gtgtcgatta cctcgcccac cccgggcctg gcgaccagaa
       61 cgaagtcctg atcggcttca tcgcggtcca cctgggacca gaaggcttcc ttcagaacgc
      121 gtttgacccg attccgggtg accgcatcgc ccagcttctt ggaaacgctg accccgaggc
      181 gaatctcgga ttcatcatcg gccgagcgat cgaaccggta gagaacgagg taacgggtcg
      241 cctttgacga cccttcgcgg taagcccgtt tgaagtctcc gctgcgggag agccggcggc
      301 gcctgggaga accagcgccg tttgctgaca tggtcagacg gcgagccgct tgcggccctt
      361 gcggcggcgg cggcgaatga cctcgcgccc gcccttggag ctcatgcggg cccggaagcc
      421 gtgggtcttt gcgcgcttgc gcttctttgg ctggtacgta cgcttcattg gagccgggca
      481 gtataagggg atcaggcgcc tcatggttcc cgccctgcgc ccgctgccag cctcctgccg
      541 ggcggaaacc cggctccaag aaattttctg cgcgatttgc gggtccggaa cgcggcctcc
      601 catggaccgg cgctatggtc ggcaggcagg gtggagattg agcagcgagc gttccccggc
      661 cagcctcgat cagccctttt ccgacatgag agcagccacc agttcaaacc gtgaaagcaa
      721 ggcaggaagc cgaaagtcgc ttcccggctc ggctcgtgcc gtgaaggaat gggagaccat
      781 ccagccgacc ctcaagcggc gcctctttga ctcggcctgg cggatgtgga tcgaaccggt
      841 tactcccgtc gcggtccgcg gaaccacgct ctacctccag gctccggatc cgattcgtac
      901 ctgggtcgag cggcgctaca tcgaggtcat aaccgagact ctcgtcgact cgagttcgca
      961 cctcaccgcg gtcagcttcg agaatccacc cgaacccgaa cgggtcccgg cgccaacccg
     1021 gccggccggt cccaacccga cccatgactt cgatcgcttc gtgatcgggc ccggcaatca
     1081 tcttgcccac gccgctgccc tggcggtcgc cgaagccccc tccgagtcat acaacccgct
     1141 cttcctgcac ggtccccccg gcctcggcaa gacccacctg ctcggtgcga tcgccaacta
     1201 cctggccctt cacgttcccg aactgaacgt cctctatacg aacgccgagt ccttcaccgc
     1261 cgaattcgtc accgctctcc gcgaccaggg aaccgacgag ttcaaacggc gctaccgcaa
     1321 tctcgacgta ctgctgatcg atgacatcca gttcatcgcc ggcaaacccc acaccgagga
     1381 agagttcttc cacaccttca atgccctgca tgacgccggc agccagatcg ttctttccgc
     1441 tgaccgctcg ccaggcgaga tctcgaccct cgccgaccgg ctcagcgacc gtttcgaatg
     1501 gggcctcacg gtgtcgatcg aagcgccgaa cctggctaca cggctcaccg ttcttcgcca
     1561 cctggttacc gaagccggac tcgaggtagc cgatcaggac gcccttaccc tgctggctcg
     1621 ccgggtgcag tccaacctcc gtcagctccg gggagcactg acccgcacga ttgccgaatc
     1681 ttcgctgaac tccgctccga tcacctccga gttgatcacc caggtctttc ccgcgaccga
     1741 atctgctccg gccatccaga tcaccccgga gacgatccgc agcacggttg ccgcccatta
     1801 caggactgac gttgaaaccc tccagtcccg ccgccgggac cggaacaccg ccacggccag
     1861 gcatgtcgcg atctacctga cccgggagat cacctcgctt tccctgcccc agatcggcgg
     1921 gctctacggg gaccgggacc acaccaccgt tctcaactcg atcg
//
LOCUS       DAOQMF010000387         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_40253_length_1962_cov_2.253277, whole genome shotgun sequence.
ACCESSION   DAOQMF010000387 DAOQMF010000000
VERSION     DAOQMF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40253_length_1962_cov_2.253277"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLV77_06915"
     CDS             <1..513
                     /locus_tag="PLV77_06915"
                     /inference="COORDINATES: protein motif:HMM:NF037213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNC15613.1"
                     /translation="PIYRGEAGIDVLNRELQQRLNPEGRKAVSDRFRIGDRLIQTRNA
                     YEYGLMNGTICFLVDDDPDEEMVVLETDDGQSVELPYAETSDLKLAYAISVHKSQGCE
                     IPVVIFVCHRSHSGMLTRPLIYTAITRAREMCVMVGDRPALENGVRRDEAGRRYSSLA
                     ARLAGTLNHR"
     gene            606..>1962
                     /locus_tag="PLV77_06920"
     CDS             606..>1962
                     /locus_tag="PLV77_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNC15614.1"
                     /translation="MAFAAAAFAAMLMLAAPASAQKLVTIDASSDYVDPSNQHLAGPV
                     TENMGPHPGKLMANVLLPDGYTPKKRYPLLLLLHGSGERFDSWADPLLGDIRNTAKGL
                     NAVIVMPEGAQGFYTDWWNEGERSGPAWEDYIRESLLPMIQSRFSIRPERRYHAIAGF
                     SMGGYGTWLTASQLPGFFGTAVPLSAFASIRTFESTLAFATAAGGTPYAQVYGDPTGF
                     YAEGHDPIALGPNLAFTNLDVFTGDGEPDPEVRPQNDPSNPGYVTDGDGVSLYLESVL
                     KTQNDAAVAAMEASGNKNVDYTVHKGSHDWEFWRPDLKAAIAKGLFRPTPARPANWSY
                     RTAASEGQAWDIYFNFSPAPTAVTTFKRTGNRLKVTGEGEMTIFDGNGCSFSADLPFT
                     RTLSEKPCRSLKLQAKGGGLRKGRTGTITVTVKGMNYTGVTKPVDAVKVEAGGASAIT
                     AY"
BASE COUNT          360 a          687 c          606 g          309 t
ORIGIN      
        1 ccgatctacc gcggcgaggc gggcatcgac gtgctcaacc gcgaacttca gcagcggctc
       61 aacccggagg gccgcaaggc ggtcagcgac cggttccgga tcggcgaccg gctgatccag
      121 acccggaacg cctacgaata cggcctgatg aacggcacga tctgcttcct cgtcgatgac
      181 gacccggatg aggaaatggt cgtgctcgag accgacgatg gccagtcggt cgaactgccc
      241 tacgcggaga ccagcgacct caagcttgcc tacgcgatct cggtccacaa gtcgcagggc
      301 tgcgagatcc cggtcgtgat cttcgtctgc caccgctccc actcgggcat gctcacccgc
      361 cccctgatct acaccgcgat cacccgcgcc cgggaaatgt gcgtgatggt gggagaccgc
      421 cccgccctcg aaaacggcgt ccgccgcgac gaagccggcc gacgctactc ctcgctggct
      481 gctcgcctgg ccggcaccct caaccacagg tgagctggct ggttctcgcg caagggctta
      541 ccatgccgcc tatgggaacc atgacgttgg ggagaagtcg ttcgttcagt gctgcctgga
      601 agctggtggc gtttgccgcg gccgcctttg ccgccatgct gatgctggct gctcctgctt
      661 cggcgcagaa gctggtcacg atcgatgctt ccagcgacta tgtggatccc tcgaaccagc
      721 atctggctgg tccggtgacc gaaaacatgg gcccccaccc cggcaagctg atggccaacg
      781 tgctgctgcc ggacggttac acgccgaaga agcgttaccc gctgctgctc ctccttcacg
      841 gttccggcga aaggttcgac tcctgggcag acccgctgct cggggacatc aggaacaccg
      901 cgaagggcct gaacgcggtg atcgtcatgc cggaaggcgc gcagggtttc tacaccgact
      961 ggtggaacga aggcgagcgc agtggcccgg cctgggagga ctacatccgc gagtccctgc
     1021 tgccgatgat ccagagcagg ttcagcatcc ggcccgagcg ccgctaccac gcgatcgccg
     1081 gattctcgat gggcggctac ggcacctggc tgaccgcctc gcagctgccg ggattcttcg
     1141 gcacggcggt gccgctatcg gccttcgcct cgatccgcac ctttgaatcg accctcgcct
     1201 tcgcgacggc ggccggcggc accccttacg cccaggtgta cggcgacccg accggtttct
     1261 acgcggaagg gcatgatccg atcgcgcttg gaccgaacct cgccttcacc aatctcgacg
     1321 tgttcaccgg cgacggtgaa cccgacccgg aggtccgccc ccagaacgat ccgtccaacc
     1381 ctggctacgt gaccgatggc gacggggtca gcctctacct cgaaagtgtg ctgaagacgc
     1441 aaaacgatgc ggccgtggcg gccatggagg cttccgggaa caagaacgtc gactacaccg
     1501 tccacaaggg cagccacgac tgggagttct ggcgccccga cctgaaagcg gcgatcgcca
     1561 agggcttgtt ccgccccacc cccgcccggc cagccaactg gtcctaccgc accgccgcct
     1621 ccgaaggcca ggcctgggac atctacttca acttcagccc ggccccgacc gcagtcacca
     1681 ccttcaagcg gaccggaaac aggctcaagg tgaccggcga gggcgaaatg acgatcttcg
     1741 acggcaacgg ctgctcgttc tcggctgacc tgcccttcac ccgcaccctg tcggagaagc
     1801 cctgccgcag tctgaagctg caggcgaagg gcggcggact gcgcaagggc aggaccggca
     1861 cgatcaccgt gaccgtcaag ggcatgaact acaccggagt gacgaagccg gtcgacgcgg
     1921 tcaaggtcga agccggcggc gcctcggcca tcaccgccta ca
//
LOCUS       DAOQMF010000388         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_40967_length_1942_cov_3.623741, whole genome shotgun sequence.
ACCESSION   DAOQMF010000388 DAOQMF010000000
VERSION     DAOQMF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40967_length_1942_cov_3.623741"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLV77_06925"
     CDS             <1..531
                     /locus_tag="PLV77_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC15615.1"
                     /translation="GEGDDAVVVERIQFEGYGPGGAAILVEALTDNRNRTSAEVRHAF
                     TKHNGSLGEPGSVAWMFETFGVVVVDGEANSEDDLMGAIDAGASDVVEEEGKLRVLAE
                     PTNLTAVRQAIEADGIEIESAGLEVIPKNTVDIEESDAGTLIKLLEAIEDQDDVNEVH
                     ANFDIPDEVLEKIAAD"
     gene            583..1074
                     /locus_tag="PLV77_06930"
     CDS             583..1074
                     /locus_tag="PLV77_06930"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNC15616.1"
                     /translation="MESEGGRWSRASWAKRIGWIFGGLVALFLVIQLVPYGRDHTNPP
                     VTRSPKFVGAQTEQIFNDSCGDCHSNLTKWPWYSNVAPVSWLVQSDVDEGRSIMNFSE
                     WDKPQPAIDELVEQINEGSMPPSKYTRIHSGTKLSDSEKQTLINGLIATYRANPPGGI
                     KGG"
     gene            1111..1644
                     /gene="ruvC"
                     /locus_tag="PLV77_06935"
     CDS             1111..1644
                     /gene="ruvC"
                     /locus_tag="PLV77_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNC15617.1"
                     /translation="MKVVMGIDPGTANLGFGVVRIEGGHMVALDGGVIETASGDPLER
                     RLGEIHSSLSRLIKWHQPVALAIEDLYFGKNVGSAMKVGQASGVSMLAAAQCDVPCFS
                     YTPQAIKTAVCGSGNAAKRQVQKMVATLLNLSEVPQPDHAADALAVAICHGGTAGRRD
                     ATEAALAGSGSSLRVAS"
     gene            1641..>1942
                     /locus_tag="PLV77_06940"
     CDS             1641..>1942
                     /locus_tag="PLV77_06940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HNC15618.1"
                     /translation="MIATVRGEVLVRRSDYVVIEAAGVGYRLAVSSETLRSVPAAGQE
                     IFLHAETISRDDSLLLYGFSSEEERDLFNMLIGVSGIGPKVAISALSGGPHRELTRA"
BASE COUNT          385 a          592 c          638 g          327 t
ORIGIN      
        1 ggtgaaggcg acgacgcggt cgtcgtcgag cggatccagt tcgagggcta cggacccggc
       61 ggtgccgcga tcctcgttga ggcgctgacc gacaaccgca accggaccag cgccgaagtc
      121 aggcatgcct tcaccaaaca caacggcagc ctcggcgagc ccggttcggt cgcctggatg
      181 ttcgaaacct tcggtgtggt cgtggttgac ggtgaagcca acagcgaaga cgacctgatg
      241 ggggcgatcg acgccggagc cagcgacgtg gtcgaggaag aaggcaagct gcgcgtcctc
      301 gccgaaccga ccaacctgac cgcggtcagg caggcaatcg aggccgacgg catcgagatc
      361 gaatcggccg ggctcgaagt catccccaag aacacggtcg atatcgagga atccgacgcc
      421 ggcaccctga tcaagctgct cgaagcgatc gaagaccagg acgacgtgaa cgaagtccac
      481 gccaacttcg acatcccgga cgaggttctg gagaagatcg ccgccgatta gagtcctctt
      541 gcaaagctgg aattgatcga atgatcgctc tatcttcggc ccatggaaag cgaaggcggt
      601 cgctggagtc gggcctcatg ggcgaaaaga atcggctgga tcttcggggg gcttgtcgct
      661 ctcttcctgg tcatccagct ggtcccctac ggcagggacc acaccaatcc tccggtcacc
      721 cggtcaccga agttcgtcgg tgcccagacc gagcagatct tcaatgactc ctgtggcgac
      781 tgccacagca acctgaccaa gtggccctgg tattccaacg tggcgcccgt ctcctggctg
      841 gtgcagagcg acgttgacga gggtcgttcg atcatgaact tctccgagtg ggacaagccg
      901 cagccggcga tcgacgaact ggtcgagcag atcaatgaag gttcgatgcc gccttccaag
      961 tacacgagga tccacagcgg aacgaagctc tccgattccg aaaagcagac cctgatcaac
     1021 gggctgatcg ccacctaccg ggccaatcca cccggcggca tcaagggtgg ctgaaagagg
     1081 catccgacgg gcggtacaga atcatgaggg atgaaagtcg taatggggat agatcccggg
     1141 acggcgaacc tcggcttcgg cgtggtcagg atcgagggtg gccacatggt cgccctggac
     1201 ggcggggtta tcgaaaccgc ttccggtgat ccgctcgaac ggcgacttgg cgagattcat
     1261 tcctcccttt cccgcctgat caagtggcat cagccggttg ccctggcgat cgaggatctc
     1321 tatttcggca agaacgtggg ctcggcgatg aaggtggggc aggcctccgg cgtttcgatg
     1381 ctggccgccg cccaatgcga cgtgccctgt ttcagctaca cgccgcaggc gatcaagacc
     1441 gcggtctgcg gttcgggcaa cgcggccaaa cggcaggtgc agaagatggt tgcgaccctg
     1501 ctcaacctca gcgaagttcc ccagccggat cacgcggctg atgccctggc ggtagcgatc
     1561 tgccatggcg gtacggctgg ccggagggat gcgaccgagg ccgccctggc cggatccggg
     1621 tcgtccctgc gggtggcgag ttgatcgcca cggtccgcgg agaggtcctg gtcaggcgct
     1681 ccgattacgt cgtgatcgag gcggccgggg tgggataccg gctggcggtc agttccgaga
     1741 cgctgcgcag cgtgccggcg gccggccagg agattttcct ccacgccgag acgatcagcc
     1801 gcgacgacag cctgctgctc tacggcttct catctgagga agaacgggat ctcttcaaca
     1861 tgctgatcgg ggtgagcgga atcggcccca aggtcgcgat ctccgccctt tcgggaggcc
     1921 cgcaccggga actgacccgg gc
//
LOCUS       DAOQMF010000389         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_41123_length_1938_cov_3.823686, whole genome shotgun sequence.
ACCESSION   DAOQMF010000389 DAOQMF010000000
VERSION     DAOQMF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41123_length_1938_cov_3.823686"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1261)
                     /locus_tag="PLV77_06945"
     CDS             complement(137..1261)
                     /locus_tag="PLV77_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC15619.1"
                     /translation="MTETPDRSEEEVEEGDGLPVFTTKGAVQTAVLIVLLIVAIYFLL
                     PKLAGFGDAIGMLGEAKPVYVGIAIGFFVLSFITYIALFRAVVGGESFPITWREAYEI
                     NMAGFAATLLFSAGGAGGVVLTYWALRQGGMERRDVGTRMVAFIVLHYLFYPLAVLVF
                     GILLGTGVLHGSTEVSLTFVPAAIALIVMLLGGALALVPADIERRIEGYARRHPGAKF
                     LARLSTVPAMVADGTRGALHLVSHPSRAGLAVVGAVGFWAANIGILWASFKAFGIAIP
                     LGVVVMGFFIGMVANLIPFVPAGVGAVDAGMIGTFVLFGFPEEQVFAAILIYRLVAFW
                     LPIPPGVAAFFQLRNRVKRWKKEGLPIDRPPRPSMIEEIL"
     gene            complement(1258..>1938)
                     /locus_tag="PLV77_06950"
     CDS             complement(1258..>1938)
                     /locus_tag="PLV77_06950"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC15620.1"
                     /translation="VEYPIRIDRFIGRGQLPNARLQKWLHQGRYGNDLTGFAAWTHWF
                     WFIQPYAAIFWIAVRNKEKFPESARQLAAVFDMGCAMYFLVPTAPPWWAAENGYIDPE
                     IADEHVKRVMLEYGEKLWGPAWTRIFGTLGSNPWAAMPSLHFATAVMAAILLSDAGGK
                     VEGALGWTYALSLGFALIYLGEHYVTDLLVGAGVVLLARKGEPIVRPVVELINGRLQR
                     LEAIAAGG"
BASE COUNT          372 a          644 c          617 g          305 t
ORIGIN      
        1 accatcccgc attggctgcg attccggtgg caccgccgat gatgatgatg ttttcgacct
       61 cggtatcgct catttcaggt tcctggtttc tcgtccgttt tcattacttt actttctgaa
      121 gtaagtgtag cgttcttcag agaatctctt cgatcatgct cggccgcggc ggcctgtcga
      181 tcggcaggcc ttccttcttc cagcgcttga ccctgtttct caactggaag aaggcggcga
      241 caccgggcgg gatcggcagc cagaacgcga ccagacgata gatcaggatc gccgcgaaga
      301 cctgctcctc cggaaagccg aagagcacga aggtgccgat catgcccgcg tcaaccgccc
      361 cgaccccggc cggcacgaag ggaatcaggt tggcgaccat cccgatgaag aagcccatca
      421 cgaccacgcc caggggtatc gcaatcccga aggccttgaa cgaggcccag aggatgccga
      481 tattggcggc ccagaacccg accgccccga ccacggcgag tccggcccgg gatggatgac
      541 tgaccagatg gagggccccc cgggtcccgt cggcgaccat cgccggcacg gtcgaaagcc
      601 gggcgagaaa cttcgccccc ggatgccgcc tcgcgtagcc ttcgatccgc cgctcgatgt
      661 cggcagggac gagggcaagg gccccgccca gcagcatcac gatcagggcg atcgccgccg
      721 gaacgaaggt cagcgacacc tcggtcgagc cgtggaggac cccggtgccg agcaggatcc
      781 cgaagaccag gaccgccagc gggtagaaga gatagtgcag gacgatgaag gcgaccatgc
      841 gggtgccgac gtcccgccgc tccatcccgc cctgccggag cgcccagtag gtcagcacca
      901 cgccaccggc cccgcccgcc gagaagagca gggtcgcggc gaaaccggcc atgttgattt
      961 cgtaggcctc ccgccaggtg atcggaaagc tctcgccacc gacgaccgcg cggaacaggg
     1021 caatgtaggt gatgaaggag aggacgaaga atccgatcgc gattccgacg tacaccggtt
     1081 tcgcctcccc cagcatcccg atcgcgtcgc cgaacccggc aagctttggc agcaggaagt
     1141 agatcgcgac gatcagcaga acgatcagga ccgcggtctg gaccgccccc ttggtggtga
     1201 agaccgggag gccgtcaccc tcctcgactt cctcctcgga acgatccggc gtttcggtca
     1261 tccgccggct gcgatcgctt ccagtcgctg gaggcgtccg ttgatcagct cgacgaccgg
     1321 ccggacgatc ggttcgccct tgcgggccag caggaccacg cccgccccga ccagcagatc
     1381 ggtcacgtaa tgctcgccca gatatatgag ggcgaaaccg agtgaaagcg cgtaggtcca
     1441 tcccagcgcg ccctcgacct tgccgccggc atctgaaagc aggatcgctg ccatcactgc
     1501 cgtggcgaag tgcagcgagg gcatcgccgc ccagggattg gagccgaggg tgccgaagat
     1561 ccgggtccag gccgggcccc acagcttctc gccgtactcg agcatcaccc gcttgacgtg
     1621 ctcgtcggcg atctcggggt cgatgtagcc gttctcggct gcccaccagg gcggcgcggt
     1681 tggaaccagg aagtacatcg cgcagcccat gtcgaagacc gcggccagct gccgggccga
     1741 ttcggggaac ttctccttgt tgcggaccgc gatccagaag atcgccgcgt agggctgaat
     1801 gaaccagaac cagtgggtcc aggcggcgaa gccggtcagg tcgttgccgt agcggccctg
     1861 atggagccac ttctgaaggc gtgcgttggg cagctgcccg cggccgatga agcggtcgat
     1921 ccgtatcggg tactcgac
//
LOCUS       DAOQMF010000390         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_41161_length_1937_cov_3.570670, whole genome shotgun sequence.
ACCESSION   DAOQMF010000390 DAOQMF010000000
VERSION     DAOQMF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41161_length_1937_cov_3.570670"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1260)
                     /locus_tag="PLV77_06955"
     CDS             complement(34..1260)
                     /locus_tag="PLV77_06955"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNC15621.1"
                     /translation="MSENLRIGLLGRGTVGSAFDELVAERADQVEAATGRRPEVTGVL
                     TRSQGDFGEILPDADVIVELMGGTDTAREHVLAALRAGVPVVTANKQLLAQHGAELFA
                     AAEEGGVQLRFEAAVAAVVPVIRVVQESFGAVEFSKVFGIVNGTTNFILSEMAATGAS
                     YAEVLKRAQELGYAEADPTEDVNGADAAAKMAILARLAFHTPVTLDQVDFEGIEDIQP
                     DDLAYAKELGLSLKLLGVAEKLEQGITVRVFPCFLYPGHPLAPIEGPFNAVMVEAPEI
                     TEVTMSGPGAGGLQTAAAVLGDVVSVAAGDARVHTADRDLPVLADVTSSFYLHLEVAD
                     EPGVLAEVAKVLSDGGVSVKSVVQRGLGDDARLVMVIHRCLESEFKAAVAQIAELDSL
                     KSGPRFIRVIEEEFTG"
     gene            complement(1257..>1937)
                     /locus_tag="PLV77_06960"
     CDS             complement(1257..>1937)
                     /locus_tag="PLV77_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15622.1"
                     /translation="NFLHATDTGRRIITRAQQEHAIAEKPGLLRKLFATEGTWEREAV
                     CVREVPTKASEAAMKDARKKGRAEVQAVRDVFGDEVKILIEPGRSLVGTAGVTIYRIG
                     TVKEIPGVRTYVAVDGGMSDNLRPMLYDAKYTAVIADRPDDPAGTTVTVAGSHCESGD
                     ILIRDVELANPRVGDILLTPATGAYGHSMSNNYNGMPRPPVIFCEDGEATVVVRRETW
                     DDLLRRDM"
BASE COUNT          314 a          664 c          629 g          330 t
ORIGIN      
        1 gtacctctcg ataagtggct gtggcatggc ttcctaacct gtgaattctt cttcgatgac
       61 ccggatgaag cgcggccccg acttgagcga gtcgagctcc gcgatctgcg cgaccgccgc
      121 cttgaattcc gattcgaggc aacggtggat gaccatcacc aggcgggcgt cgtcgccgag
      181 cccgcgctgg acgacgctct tgactgacac gccaccgtcg gaaagcacct tggcgacttc
      241 agccaggacc cccggttcgt cggcaacctc gaggtggagg taaaaggatg aagtgacgtc
      301 ggccagcacc ggcaggtcgc ggtcggcggt gtggacgcgg gcgtcgccgg ccgcgaccga
      361 aaccacgtca ccgagcacgg cggcagcggt ctgaagacca ccggcgccgg ggccggacat
      421 ggtcacctcg gtgatttcgg gcgcctccac catcaccgcg ttgaacggac cctcgatcgg
      481 cgccagggga tggcccgggt aaaggaagca gggaaagacc cggacggtga tcccctgctc
      541 cagcttctcg gcgaccccga gcagcttcag cgaaaggccg agctccttgg cgtaggcgag
      601 atcatccggc tggatgtcct cgatcccctc gaagtcgacc tgatcaaggg tgaccggggt
      661 gtgaaaggcg agccgggcga ggatcgccat cttcgcggcg gcgtcggccc cgttcacatc
      721 ctcggtcggg tcggcctcgg cgtaaccgag ttcctgggcc cgcttcagca cctcggcgta
      781 ggaggcaccg gtggcggcca tctcggaaag gatgaagttc gtcgtcccgt tgacgatgcc
      841 gaagaccttg ctgaactcga ccgcgccgaa gctttcctga accacgcgga tcaccggcac
      901 gaccgcagcc actgcggcct cgaagcggag ctggaccccg ccttcttcgg cggcggcgaa
      961 gagctcggcc ccgtgctggg cgagcagttg cttgttcgcg gtgaccaccg gcacgccggc
     1021 cctgagcgcc gccagcacat gttcgcgggc ggtgtcggtg ccgcccatca actcgacgat
     1081 cacgtcggca tcgggcagga tctcgccgaa atcaccctgg ctgcgggtca ggaccccggt
     1141 cacctccggc cgacggccgg tcgccgcctc gacctggtcg gcccgttcgg caacaagttc
     1201 atcgaaggcg gagccgacgg ttccccgtcc gagcaggccg atgcgcaggt tctcgctcac
     1261 atgtccctcc tgagaaggtc atcccaggtt tcgcgtcgca ccaccacggt ggcttcgccg
     1321 tcttcacaga aaatcacggg cggccgcggc attccgttgt agttgttcga catcgagtgc
     1381 ccgtaggcgc cggtcgccgg ggtaagcagg atgtccccca cccgggggtt cgcgagctcg
     1441 acatcacgga tcaggatatc tccggactcg caatgtgaac cggcgacggt gacggtggtc
     1501 ccggcggggt catcgggccg gtcggcgatc accgccgtgt acttcgcgtc atagagcatc
     1561 ggccggaggt tgtcggacat gccgccgtcc accgcgacat aggtccggac cccggggatc
     1621 tccttcacgg tcccgatccg gtagatcgtc accccggcgg taccaaccag cgagcggccc
     1681 ggctcgatca ggatcttgac ctcgtctccg aagacgtcgc gaaccgcctg gacctccgcg
     1741 cgccccttct tgcgcgcatc cttcatcgcc gcctcgcttg ccttggtcgg cacctcgcgc
     1801 acgcagaccg cttcgcgctc ccatgtgcct tccgtcgcga acagcttacg gagcaagccg
     1861 ggcttctccg cgatcgcgtg ctcctgctgc gcacgcgtga tgatccgacg gcccgtgtcg
     1921 gttgcgtgga ggaagtt
//
LOCUS       DAOQMF010000391         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_41558_length_1927_cov_2.545940, whole genome shotgun sequence.
ACCESSION   DAOQMF010000391 DAOQMF010000000
VERSION     DAOQMF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41558_length_1927_cov_2.545940"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLV77_06965"
     CDS             <1..184
                     /locus_tag="PLV77_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15623.1"
                     /translation="FGPALKNGNGFGELKIEDVSPSESGAVVTFDKDPPGDVPLIKKD
                     SRWYMDLAGAAESASS"
     gene            258..1544
                     /locus_tag="PLV77_06970"
     CDS             258..1544
                     /locus_tag="PLV77_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC15624.1"
                     /translation="MKTSAAIERTGFSQVGVVGMGYVGLPLAVAFAEAGCQVIGLDAD
                     PTKVASLNRSTTYIEDVPSSLLESIGDGIRATVDFDELAECDAIVICVPTPLTGSSEP
                     DLTYLESAGENLAEILAPGQLVVLESTTYPGTTRDLLAGKLEEGGLKAGEDFHLAFSP
                     ERIDPGRTDYTVKTTPKLVGGISPECTDRAATLYETVCDQVVRLSGPEAAELSKLLEN
                     IFRSVNIALVNELAQLCDRLDVDVWEVVEAASTKPFGFMPFEPGPGMGGHCLPIDPFY
                     LAFTARQNDFYPEFIELAGKINQSQPRFCVRRIERILNNQMRSVKDSKILILGVSYKA
                     GVGDTRESPAIKIIHLLAELGADLRYHDPYVPELKDAGLEHSEDLDAALEEVDLAVIV
                     TSHPEVDLDTVVDKSKSLLDLRGVTRKHEGAHITRL"
     gene            1616..>1927
                     /locus_tag="PLV77_06975"
     CDS             1616..>1927
                     /locus_tag="PLV77_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15625.1"
                     /translation="MKAAIFVHPSGLCESESVGSGTRIWAFAHVLEGAVVGKDCNVCD
                     GAYIESDVVIGDRVTIKNGVMLFDGARIGDDVFLGPGVVFTNDLNPRSRLRDGTDHLL
                     AI"
BASE COUNT          415 a          580 c          562 g          370 t
ORIGIN      
        1 gttcggtccg gcgctaaaga acggaaacgg cttcggtgaa ctgaagatcg aggacgtttc
       61 gcccagcgaa agcggagcgg tcgtgacctt cgataaggat ccgcccggcg acgtaccatt
      121 gatcaagaag gactcacgct ggtatatgga cttggctggc gcggctgaat cggccagttc
      181 ctagcctagg ctccagtact tcaccaacac tcctgcaaag gggcattttg cacacccgag
      241 agagaccgac acacgaagtg aaaacctctg ccgctataga aagaaccggc ttcagccagg
      301 taggtgtcgt cggaatggga tatgtcgggc tgccactggc agtcgccttc gccgaagccg
      361 gatgtcaggt gatcggcctg gatgccgatc cgaccaaggt cgcaagcctg aaccgctcga
      421 ctacatacat cgaggacgta ccgtcgtcct tgctcgagag catcggcgat ggcattcgcg
      481 ccaccgtcga cttcgacgaa ctggccgaat gcgacgcgat cgtcatctgc gtcccgaccc
      541 cactcaccgg gtcaagcgaa ccagacctca catatctgga gtctgccggt gagaacctcg
      601 ctgagatcct tgctcccggt caactcgtcg ttctcgaatc gacgacttac cccggcacca
      661 ccagggatct tcttgccggg aaactggagg agggaggact gaaggccgga gaggacttcc
      721 acctagcctt ctccccggag cggattgatc caggtcgtac cgactacacg gtcaagacca
      781 caccgaaact ggtcggagga atctcgccag aatgcacaga ccgcgccgca accctttacg
      841 aaacggtgtg cgaccaggtt gttcggctgt cggggccgga agccgccgag ctttcgaagc
      901 tgctcgagaa cattttccgt tcggtcaaca tcgccctcgt taacgaactg gcccagcttt
      961 gcgaccggct tgacgtcgat gtctgggagg tggtggaagc ggcctcaacc aagccattcg
     1021 ggttcatgcc gttcgagccc ggccccggca tgggaggaca ctgcctgccg atcgacccat
     1081 tctacctcgc cttcaccgcc cggcagaacg atttttaccc cgagttcatc gaacttgctg
     1141 gcaagatcaa ccagtcgcag ccgcgcttct gcgtcagacg gattgagcga attctcaata
     1201 atcagatgcg atcagtcaag gactcgaaga tcctgatcct cggagtcagt tacaaggccg
     1261 gggtcggaga cacccgggaa tcgccggcaa tcaagatcat ccatctgctg gcggagctcg
     1321 gagccgacct ccgctatcac gacccgtatg tgccggagtt gaaggacgcc gggttggagc
     1381 attcggaaga cctcgatgct gctctcgaag aggtcgacct ggcagtcata gtcacctccc
     1441 accccgaggt cgatctcgac accgtcgtcg acaagagcaa gtcgctactc gacctgcgtg
     1501 gcgtcacccg gaagcacgaa ggcgcccaca tcacgcggct atagccggga tgccgctacg
     1561 ggtgttttcg cccggggtcg tctccgaaag ttcaaaaacc cgtaatcatt gggtcatgaa
     1621 agctgccatt ttcgttcacc catccggact ttgcgaaagc gaaagcgttg ggtccgggac
     1681 ccgcatctgg gcgtttgccc atgtacttga gggagcggtg gtaggaaaag actgcaatgt
     1741 ctgcgatggc gcttacatcg aatccgatgt ggtcatcggg gaccgggtca cgatcaagaa
     1801 cggggtcatg ctgttcgatg gcgcgcggat aggagacgat gtcttcctgg ggccgggtgt
     1861 cgtgttcacc aacgatctca acccccgatc gcgtctgcgc gatggaacag accacctgct
     1921 cgcgatc
//
LOCUS       DAOQMF010000392         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_41622_length_1925_cov_5.155615, whole genome shotgun sequence.
ACCESSION   DAOQMF010000392 DAOQMF010000000
VERSION     DAOQMF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41622_length_1925_cov_5.155615"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLV77_06980"
     CDS             <1..640
                     /locus_tag="PLV77_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15626.1"
                     /translation="FAGPKNFTGFSGLAIGPNGRLYAGVSLNQKHDHTADPSLYGNSV
                     VSMGLAGKNVKVVATGLRQPWMMTFAPGVRDPFVSVLGPDTPVNNGAPDLIVKARPGS
                     DFGFPECDWLIEDECDGLAEPLVRLEAQPGKKGSLNQQSPMGIGAIGKRLYVALFSGT
                     AKAGIQVMTMKTDGSGIKPFMTGFVAPVLSVATHKGYVYAGDLTGTIYKVKG"
     gene            794..1759
                     /locus_tag="PLV77_06985"
     CDS             794..1759
                     /locus_tag="PLV77_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15627.1"
                     /translation="MAHTATAAAPGLPVAENGKPVKVFATGVAVPTQIAFKGKTAFIS
                     GGPENNKPGGLYYVSPGSKKAKRVPGTPPIAFGVTWHHGHLYANFGTVIRVFHGWTGK
                     RFRFSRTLVSFKPKNFTNFSGLEFGPNGRLYTGVGLQFDAKAGTKAYANSVISIDKRS
                     GRIRTVSTGLRQPWMLTFVKGSRSPLVTVLGQDSPKGTLAPDLIVKATPGANFGFPRC
                     NWSNVKTCARLHFKKPVMTFAAAEPSPSPMGITAKGEKLFVALFGGLSTDEGPIGPEI
                     VTTSSHGGIVRNFVTGFKAPVLLARYHGGYLYMGDLSGKVYRVKA"
BASE COUNT          355 a          651 c          592 g          327 t
ORIGIN      
        1 cttcgccgga ccgaagaact tcaccggctt cagcggactg gcgatcggcc cgaacggacg
       61 cctctacgcc ggcgtctcgc tcaaccagaa gcacgaccac accgccgacc cctcgctcta
      121 cggcaactcg gtcgtctcga tggggctggc cggcaagaac gtgaaggtgg tcgcgaccgg
      181 cctccggcag ccgtggatga tgaccttcgc ccccggggtc agggacccct tcgtctcggt
      241 cctcggaccg gatacgccgg tcaacaacgg tgccccggac ctgatcgtca aggcccggcc
      301 cggttccgac ttcggcttcc cggagtgcga ctggctgatc gaggatgaat gcgacggcct
      361 ggccgaaccg ctggtccggc tcgaggccca gcccggcaag aagggctcct tgaaccagca
      421 gtccccgatg ggcatcggcg cgatcggcaa gcggctctac gtcgccctct tcagcggcac
      481 cgccaaggcc ggcatccagg tgatgacgat gaagaccgac ggaagcggca tcaagccgtt
      541 catgaccggc ttcgtcgctc cggtcctctc ggtcgctacc cacaagggtt acgtctacgc
      601 cggcgacctc accggcacca tctacaaggt caagggctag tccccggagt ccaccaaccc
      661 accttcttgt tgcgggtgtc cgcggcaggt aaggtgggtt ggctggatcc aaaaccgggg
      721 aaagtgagac cgaatgatga agctcagggg ttcattggcg gcgctagcag ctgccttgat
      781 cggattggcc gcgatggccc acaccgcgac tgcggcagcg cccgggttgc cggttgccga
      841 aaacggcaag ccggtcaagg tcttcgcgac cggtgtcgcg gtaccgaccc agattgcctt
      901 caagggcaag acggccttca tctcgggcgg acctgaaaac aacaagcccg gcggtctcta
      961 ctacgtttcg cccgggtcga agaaggccaa gcgggtgccc ggcacgccgc cgatcgcctt
     1021 cggcgtgacc tggcaccacg gtcatctcta cgccaacttc ggcaccgtga tccgggtatt
     1081 ccacggctgg accggcaagc ggttcaggtt ttcccggacc ctggtctcat tcaaaccaaa
     1141 gaacttcacc aacttcagtg gcctcgagtt cggacccaac ggccggctct acacgggtgt
     1201 cggcctgcaa ttcgacgcca aggccggcac caaggcctat gccaactcgg tcatttcaat
     1261 cgacaaacga agcggcagga tccgcacggt ttccaccggc cttcgccagc cctggatgct
     1321 gaccttcgtg aagggaagcc gcagcccgct ggttaccgtg ctcgggcagg acagcccgaa
     1381 gggcactctg gcgccggacc tgatcgtcaa ggcgaccccg ggagccaact tcggtttccc
     1441 acgctgtaac tggtcgaacg tgaagacctg cgccaggctt cacttcaaga agccggtcat
     1501 gacgttcgcg gcggccgaac cttcgccttc tccgatggga atcaccgcca agggggagaa
     1561 gctctttgtg gctctgttcg gcggcctcag caccgatgag ggtccgattg gtccggagat
     1621 cgtcacgacc agctcacacg gaggcatcgt tcggaacttt gtcacaggct tcaaggcgcc
     1681 cgtccttctg gcccgctacc acggcggcta cctctacatg ggtgacctgt ccggaaaggt
     1741 gtaccgggtc aaggcctagc cgacacagca gcccggccgg agccgccgga gctcagccgc
     1801 tgactgcctc gtccaggcgg tcgtcgcccc aatagacctg gccgttgacg aggaaggaag
     1861 ggacgcctgt cacaccgagg tcgccggctc gccgggtggc ctgcttcagg gcatccttca
     1921 cttca
//
LOCUS       DAOQMF010000393         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_41628_length_1925_cov_4.012834, whole genome shotgun sequence.
ACCESSION   DAOQMF010000393 DAOQMF010000000
VERSION     DAOQMF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41628_length_1925_cov_4.012834"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1312
                     /locus_tag="PLV77_06990"
     CDS             164..1312
                     /locus_tag="PLV77_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC15628.1"
                     /translation="MSDAQYALTQDQLDFLEVIRQMVVEKVTPRAHDIDESGEFPQDI
                     RELFAEHDLFSLPFEEKHGGTGTGELMLCAAIEEIAKGCASSSLMVAAQDLSSLPMKL
                     AASDELKDRILPRMASGEWLGAFCLSEPDAGSDPGAMRTRATKVDGGWKLNGAKNWIT
                     NSGVADVYVTFAVTDPDVKFSRGITCFAVMGDADGFRIDALEKKMGMKGSPTGQPVYE
                     DVFVPDEDVIGEVGKGFAVAMQTLDRSRLGIGAQALGIAQGATDYAADYAKERIAFGK
                     PINQLQGIQFKLADMETKTAAARELLYKAASMAETSHPLLGKYSAMAKLFASDVAMEV
                     TTEAVQVLGGYGYVREYPVERMMRDAKLTQIYEGTNEIQRVVIARTLA"
BASE COUNT          351 a          643 c          604 g          327 t
ORIGIN      
        1 acaccggtcc gaaggctcga tgaagtcggc gccacccgca atccgcgaat tcgccagaac
       61 ccttcctagg ccgccctgcc acaccggaaa tccggcgcct gccgatcgca ggtagatttt
      121 ccaacggcct tttttcaatc tttgaccgaa gcggagtgac cgaatgagtg atgcccagta
      181 cgccctgacc caggaccagc tcgatttcct cgaggtgatc cggcagatgg tggtcgagaa
      241 ggtcaccccg cgggcccatg acatcgacga gtccggcgaa ttcccgcagg acatccgcga
      301 gctctttgcc gagcatgacc tgttcagcct gcccttcgag gagaagcacg gcggtaccgg
      361 caccggcgag ctgatgctct gcgccgcaat cgaggagatc gccaagggct gtgcctcgtc
      421 ttcgctgatg gtcgctgccc aggatctctc ctcgctgccg atgaagctgg ccgcctccga
      481 tgagctcaag gaccggatcc tgccgcgcat ggcctcgggc gagtggctcg gcgccttctg
      541 cctctcggag ccggatgccg gttccgaccc gggcgcgatg cgcacccgcg cgaccaaggt
      601 cgacggcggc tggaagctga acggcgccaa gaactggatc accaactccg gtgtcgccga
      661 tgtctatgtg accttcgcgg taaccgaccc ggacgtgaag ttctcccgtg gcatcacctg
      721 cttcgcggta atgggtgacg ccgatggctt ccgcatcgac gcgctcgaga agaagatggg
      781 catgaagggc tcgccgaccg gccagcccgt ctacgaggat gtcttcgtcc ccgatgagga
      841 cgtgatcggc gaggtcggca agggcttcgc ggtcgcgatg cagaccctcg accgctcccg
      901 gctcgggatc ggcgcccagg ccctcggcat cgcccagggc gcaaccgact acgcggccga
      961 ttacgccaag gagcggatcg ccttcggcaa gccgatcaac cagctccagg gcatccagtt
     1021 caagctcgcc gacatggaga ccaagaccgc tgccgcccgc gaactgctgt acaaggccgc
     1081 ctcgatggcc gagaccagcc acccgctgct cggcaagtac tcggcgatgg ccaagctttt
     1141 tgcctccgac gtcgcgatgg aagtcaccac cgaggccgtc caggtcctgg gcggctacgg
     1201 ctacgtccgc gagtacccgg tcgagcgaat gatgcgcgac gccaagctga cccagatcta
     1261 cgagggcacc aacgagattc agcgagtagt aatcgcccgc accctggcgt agcgggcggc
     1321 gccaggaacc accagccgtc cgctggcttc gtcgtcggcc ggaaacgaca gctcacgtac
     1381 ccgcgtacta tcgcggtcat ttccggcctg tctccttgcc agcgaacggc tggaggcccc
     1441 tggcatccac ccgctcacgg ctggaggaga ccacgacccc gctcacggct ggtggtgttg
     1501 gggcatttcg cgagatgcgg tttgtcggca tatatcgccg acaaacagca tctcgatcga
     1561 ttcctgaccg tgactcggtg gccggtgacg caggtttgcg acctcagagg ttgcaaacct
     1621 gcggcaccag cctggatagg gacgcgaatt cggccctgga gccggcccgg accctgggtt
     1681 ccttcaactt cccccttcac ttgccctgtt ccaccgcagt gggtcggatt tccgtggcag
     1741 cagctggaaa tccgacccac cggaccatgc gaaccggggg ttggacaccg cagcgggtcg
     1801 taaaaccggt cctcgggccg aaaatccgac ccacgagacc tgcgaatcag ttccggaggt
     1861 gccagtgggt cggattttcg gggcgggtga cggaaatccg acccactggg ggatcgggtg
     1921 gggtt
//
LOCUS       DAOQMF010000394         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_41846_length_1920_cov_3.153887, whole genome shotgun sequence.
ACCESSION   DAOQMF010000394 DAOQMF010000000
VERSION     DAOQMF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41846_length_1920_cov_3.153887"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLV77_06995"
     CDS             <1..186
                     /locus_tag="PLV77_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15629.1"
                     /translation="LGQTMVYYINPAGPNQPHLEPVLQPGMDLMDDLVPDEPLISAEK
                     PIDSPQGLPREVRGPGK"
     gene            212..>1920
                     /locus_tag="PLV77_07000"
     CDS             212..>1920
                     /locus_tag="PLV77_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC15630.1"
                     /translation="MNRVFAVLAFVVAALQIGVAPASADPAPDLPAGPVLATDDWPND
                     PNFARCELQDPVSGCQDSEQWNLYGPMDGLCLAPGGAVFDQPRPDGGLPCWARNATDP
                     ESSSGINMTGAWAQGNIGRDDVLVAYVEGGVNYSANGIKDGLDSIYLNAGELPYPQGW
                     DGQDLGRYDFDGNGEFDIRDYAKDPRVNPACVSPTTQFTTYQEGTTRGCADDGTHEYL
                     HSVNIGGPLTPYLSPEDLIAVFGHCKITDHRIESCPADARIDNDGNGYPNDVSGWNAY
                     QNTNDPQSEDFNYGHAPGLIGEMGGEADNGYAGVGNCRNCRVVPIKSGAESVGKADGL
                     APAITYAADINVTAMSTVVVSYAYSSALKAAVKHAVDKGILMSMDSNDFDSMDHTDGH
                     LFDDVFPGNGLAQDIEGKNAQTFRARSNVTSYGTHSIFSGGESTTSGATPFQAAFLAM
                     TQSAALNAVDENIIPRKLTPNEIKQVLINTASEILPQRDTPDVANQWPGNPDSKTDST
                     HTAWSTQYGYGRVNIGKATKLIMDGRVPPTADIDSPSWYKYVDPTKGGKLQVKGKVAP
                     SAWG"
BASE COUNT          384 a          677 c          562 g          297 t
ORIGIN      
        1 ctgggccaga cgatggtcta ctacataaac ccggccggac cgaatcaacc ccacctcgag
       61 ccggttttgc aaccagggat ggacctgatg gatgacctgg tacctgacga accactgatt
      121 tcggccgaaa agccgataga ttccccgcag ggtctgcctc gggaggtgag gggaccaggg
      181 aaatgagtac caagggagga atgtcttgtt cgtgaaccgc gtctttgccg tgcttgcctt
      241 tgtggtggcc gctttgcaga tcggggtcgc tcccgcgtcg gccgaccccg cacccgacct
      301 gccagccgga ccagtgctgg cgacggacga ctggccgaat gacccgaact tcgcgcgctg
      361 cgaactgcag gatccggttt ccggctgcca ggacagcgag cagtggaatc tgtacggacc
      421 gatggacggc ctatgcctcg cccccggcgg cgccgtgttc gaccagcccc gcccggacgg
      481 cggcctccct tgctgggccc gcaacgcgac cgatcccgaa agctcttccg gcatcaacat
      541 gaccggcgcc tgggcccagg gcaacatcgg tcgtgacgac gtcctggtcg cctacgtcga
      601 gggcggggtc aactacagcg ccaacgggat caaggacgga ctcgactcga tctacctcaa
      661 cgccggcgag cttccctacc cccagggctg ggacggccag gacctcggcc gctatgactt
      721 cgacggtaac ggcgagttcg acatccgcga ctacgccaag gacccgcggg tcaaccccgc
      781 ctgcgtttcc ccgaccaccc agttcaccac ctaccaggag ggcacgaccc gcggctgcgc
      841 cgacgacggc acccacgagt acctccactc ggtcaacatc ggcggtccgc tcactcccta
      901 tctgtccccc gaagacctga tcgctgtgtt cggccattgc aagatcaccg accaccggat
      961 cgaatcctgc ccggctgacg ccaggatcga caacgacggc aacggctacc cgaacgacgt
     1021 ttcgggctgg aacgcctacc agaacaccaa cgacccccag tccgaagatt tcaactacgg
     1081 tcacgcgccc ggcctgatcg gcgagatggg tggcgaggca gacaatggct acgccggcgt
     1141 cggcaactgc cgcaactgcc gcgtggtccc gatcaaatcc ggggccgaat ctgtcggcaa
     1201 ggcggacggt ctcgctccgg cgatcaccta cgcagctgac atcaacgtga cggcgatgtc
     1261 caccgtggtg gtgtcctacg cctattcgag cgcgctcaag gccgcggtca agcatgcggt
     1321 tgacaagggc atcctgatgt cgatggactc aaacgacttc gactcgatgg accacaccga
     1381 cggccatctc ttcgatgacg tcttccccgg caacgggctc gcccaggaca tcgaagggaa
     1441 gaacgcccag accttccggg cccgctcgaa cgtgaccagt tacggcactc actcgatctt
     1501 ctccggaggc gagtccacta cttcgggcgc cacccccttc caggcggctt tcctcgcgat
     1561 gacccagtca gccgccctga acgcggtcga cgaaaacatc atcccccgca agctgacccc
     1621 gaacgagatc aagcaggtgc tgatcaacac ggccagcgag atcctccccc agcgggacac
     1681 cccggacgtg gccaaccagt ggcccggaaa cccggactcg aagaccgatt caacccacac
     1741 cgcctggtcg acccagtacg gctacggccg ggtcaacatc ggcaaggcga ccaagctgat
     1801 catggacggc cgggttcccc cgaccgccga catcgattcg ccctcctggt acaagtacgt
     1861 tgatccgacc aagggcggca agctccaggt gaagggcaag gtcgcgcctt ccgcctgggg
//
LOCUS       DAOQMF010000395         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_41962_length_1917_cov_3.984425, whole genome shotgun sequence.
ACCESSION   DAOQMF010000395 DAOQMF010000000
VERSION     DAOQMF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41962_length_1917_cov_3.984425"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="PLV77_07005"
     CDS             complement(<1..1479)
                     /locus_tag="PLV77_07005"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNC15631.1"
                     /translation="MSDQYQGETRFIFVTGGVLSALGKGIASASIGRLLVSRGFRIQI
                     QKFDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDENTSRASNVTAGAVY
                     NSVLRRERRGDYLGGTVQVIPHITDEIKNRILIVAETKQVDFVITEIGGTVGDIESLS
                     FLEAIRQLYTDLGRRRAMFIHLTLVPYIVHAGEMKTKPTQHSVNELRRIGIQPDALIC
                     RSVSGLDRDIRKKIAQFASLPTDSVISGQDVDNVYKIPLMYRAEGLDDFILEHFNMED
                     APPPDLSEWEAMLRMAESTEGTVRIALVGKYIQLADSYKSVIEALEHGGIHNGVDVDI
                     DLVDSEDFDISRLEGADGILIPGGFGERGVEGKIEAARYARENKIPYLGICLGMQIAV
                     IEFARHIAGMDGANSAEFDPETPFPVVDLLPEQKEVSDMGGTMRLGADPVKLHEGTRA
                     LEIFGEPVIYKRHRHRYEVNNLLRTRLEDQGLICSGTSPDERL"
     gene            complement(1476..>1917)
                     /locus_tag="PLV77_07010"
     CDS             complement(1476..>1917)
                     /locus_tag="PLV77_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15632.1"
                     /translation="YVVTGTGNEWPSYIADVALLILTTNLFNLLDLRGGRVEKVLFLM
                     VIGLCLGGWELFPIELTGVFLGPFAVGIFWTLRDRAMLGDTGSNLAGAIAGVCLLTGL
                     DETGRLIALAAVILLTIYGEFRSISKTIDRVPPLRFIDSLGRVK"
BASE COUNT          374 a          588 c          587 g          368 t
ORIGIN      
        1 cagacgctcg tccggcgagg tgccggagca aatcaacccc tggtcctcaa gacgggtgcg
       61 aaggaggttg ttgacctcgt agcgatgtcg gtggcgcttg tagatcacag gttcgccgaa
      121 tatctccagg gcccgggtgc cctcgtggag cttgaccgga tccgccccca gcctcatcgt
      181 cccgcccatg tccgagacct ccttctgttc cggcagcagg tcgaccaccg ggaagggagt
      241 ttccggatcg aactcggccg agttggcgcc gtccatgccg gcgatatgcc gggcaaactc
      301 gatcaccgcg atctgcattc cgagacagat gcccaggtag gggatcttgt tctcgcgtgc
      361 gtatcgggca gcctcgattt tgccttccac gccgcgctcg ccgaatccgc ccgggatcag
      421 gatcccgtcg gccccctcca gcctcgaaat atcgaaatcc tcggagtcga ccagatcgat
      481 atccacatcc accccgttgt ggatgccgcc gtgctccagc gcctcgatca ccgatttgta
      541 tgagtcggct agctggatgt atttgccgac cagggcaatc cggaccgtgc cctcggtgct
      601 ctcggccatc ctcagcatgg cctcccactc ggagagatca ggcggcgggg catcttccat
      661 gttgaagtgc tcgaggatga agtcgtcaag accttccgcc cggtacatca gggggatctt
      721 gtagacgttg tcgacatcct gacccgagat gacactgtcg gtcggcagtg aagcgaactg
      781 ggcgatcttc ttgcggatgt cccggtcgag cccggaaacc gaccggcaga tcagggcatc
      841 cggctgaatg ccgatgcggc gcagttcgtt gaccgaatgc tgggtgggct tggtcttcat
      901 ctcgcccgcg tggacgatgt atggcacgag ggtcaggtgg atgaacatgg cccggcgccg
      961 gccgagatcc gtgtagagct gacggatcgc ctcgagaaag gaaagcgact cgatgtcgcc
     1021 cacggtgcca ccgatctcgg tgatcacgaa atcgacctgt ttagtttcgg ccacgatcag
     1081 gatccggttc ttgatctcat cggtgatgtg agggatcacc tggactgtgc cgccaaggta
     1141 gtcgccgcgg cgctcgcgcc tgagcaccga gttgtagacc gctccggcgg tgacgttcga
     1201 agcccgggaa gtgttctcgt cggtaaaccg ctcgtagtga ccgagatcga ggtcggtctc
     1261 cgccccgtcc tcggtcacaa agacctcacc gtgctggaag gggctcattg tgcccgggtc
     1321 gacgttcagg tacggatcga acttctggat ctggatcctg aacccgcgag aaacgaggag
     1381 acgaccgatc gaagccgaag cgatcccttt gccgagagcc gaaagcaccc cgccggtgac
     1441 gaagatgaat cgggtttctc cttgatactg atcgctcact tgactctccc gagggagtct
     1501 ataaatcgca gcggcggcac ccggtcgatt gtcttcgaga tcgaacggaa ctctccgtaa
     1561 atcgtgagaa gaatcactgc cgcgagcgcg atcagccggc cggtttcatc gagtccggtc
     1621 agcaggcaga ccccggcgat cgccccggca aggttcgagc cggtgtcccc gagcatcgcc
     1681 cggtcacgaa gcgtccagaa gattccgacc gcgaatggtc cgaggaagac cccggtcagt
     1741 tcgatcggga acagctccca accgccaaga caaagcccga tcaccatgag aaaaagcacc
     1801 ttttcgaccc ttccgccacg caggtcgagc aggttgaaga ggttggtggt caggatgagg
     1861 agcgccacgt cggcgatgta ggaaggccac tcgtttccgg ttccggtcac cacatag
//
LOCUS       DAOQMF010000396         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_42064_length_1914_cov_4.075309, whole genome shotgun sequence.
ACCESSION   DAOQMF010000396 DAOQMF010000000
VERSION     DAOQMF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42064_length_1914_cov_4.075309"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /gene="yaaA"
                     /locus_tag="PLV77_07015"
     CDS             complement(<1..580)
                     /gene="yaaA"
                     /locus_tag="PLV77_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNC15633.1"
                     /translation="MLILLPPSEGKASPSGVSCLDLDSLAFAEYLSPKRAELIAALEK
                     LGKAPEKKGIEALGISKGQAGDIGRDAELLSSPVAPASKVYTGVLYDRLGLDSLPAAG
                     KRRAAKNLLISSALWGFLAPADRIPYYRFSMGARLPKIGGLPAYWRESLREAMEGAGH
                     DKEGNLVLDMRSGAYSAAWKPKQARLVTVRGFT"
     gene            complement(647..1306)
                     /locus_tag="PLV77_07020"
     CDS             complement(647..1306)
                     /locus_tag="PLV77_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC15634.1"
                     /translation="METGAESAAEKLTLVLVDDHVVVRSALKVLLESEPDLEVVAEAG
                     TADDAIRYVGAHKPDVLVLDLNMPGRPSLEAIPDIQAASPDTKIVILTMRNEPAFARQ
                     ALQAGVMGYVLKEAADTELVLAVRRAADGDTYLQPSLYQHLADKDKAEGRTGRTLSDR
                     EVEVMRLIALGHTNAEIADRLFLSVRTVESHRASIQKKLDIGSRAELVRYALDQGLIS
                     A"
     gene            1417..>1914
                     /locus_tag="PLV77_07025"
     CDS             1417..>1914
                     /locus_tag="PLV77_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC15635.1"
                     /translation="MNEATVSLDEARLRRLIEVGRSLVVESDPEAVINDALEAARELT
                     GARYAALGVLDSRRSELEQFLTRGIEAKLREQIGHPPHGGGVLGLLIEEPEPLILDDV
                     SLHPRSQGFPPGHPEMKSFLGVPVKIRGEAWGNLYLTEKEEGTFDDADIQSVVILAEW
                     IAIAID"
BASE COUNT          320 a          637 c          634 g          323 t
ORIGIN      
        1 cggtgaagcc ccggaccgtg accagccggg cctgcttcgg cttccaggcg gccgagtacg
       61 ccccggagcg catgtcaagg acgaggtttc cctccttgtc gtggccggcc ccctccatcg
      121 cctcgcggag cgactcccgc cagtaggcgg gcaggccgcc gatcttcggc agcctggcgc
      181 ccatcgagaa gcggtagtag gggatccggt cggccggggc gaggaagccc caaagcgccg
      241 aggagatcag caggttcttc gcggcccttc gcttgccggc cgcgggaagg gaatcgaggc
      301 caagccggtc gtacagcacc ccggtataga ccttcgaggc cggagcgacc ggggaactga
      361 gtagttcggc gtcccgcccg atgtccccgg cctggccctt cgaaatgccc agcgcctcga
      421 tgcccttctt ctccggggcc ttgccaagct tttccagagc agcgatcaac tccgcccgct
      481 tcgggctcag gtactcggca aaggccagtg agtcgagatc gagacaagac acgcccgagg
      541 gagaagcctt gccctcggaa ggaggtagca ggatcagcac gccgtgaatc taccgacgga
      601 gcccctccca accatccctg ggcggtgccg actcccgtca agcgcgttac gcgctgatca
      661 gcccctgatc cagtgcgtaa cgcaccagtt cggcgcggct gccgatgtcg agcttcttct
      721 ggatgctggc ccggtggctt tccaccgtgc ggacgctgag gaaaagccgg tcggcgatct
      781 ccgcgttggt gtggccgagc gcgatcaggc gcattacctc gacctcgcgg tcgctcaggg
      841 tacggccggt tcggccttcg gccttgtcct tgtcggccag gtgctggtag agggaaggct
      901 ggagataggt gtcgccgtcg gccgcgcgcc ggaccgcgag gaccagttcg gtgtcggcgg
      961 cctccttgag cacgtatccc atgaccccgg cctgaagggc ctgccgcgcg aaagcgggct
     1021 cgtttcgcat ggtcaggatc acgatcttcg tgtccgggga ggcggcctgg atgtccggga
     1081 tcgcctcgag gctggggcgg cccggcatgt tgaggtcgag caccagtaca tccggtttgt
     1141 gggcaccgac ataacggatc gcgtcatcgg cggtgccggc ctcggccacc acctccaggt
     1201 cgggttcgga ttcgagcagg accttgaggg ccgatcgcac gacgacgtga tcatcgacca
     1261 gcacgagagt cagcttctcc gcagcgcttt ccgcacccgt ttccatcaag ggaaggctac
     1321 ccgccggggc gggttccatt tccgtacctt tccgcagact tcgatttacc agggagggga
     1381 gcggaatccg gcaggcctgc ctctacaatc cccggcgtga acgaagcgac cgtcagtctt
     1441 gatgaggctc gcctgcgccg gttgatcgaa gtcggccgca gcctggtcgt cgagtccgac
     1501 ccggaagcgg tgatcaacga cgcgctggag gcggcccgcg aactgaccgg ggcgcgctac
     1561 gcggcactcg gagttctcga cagccgccgc tccgaactcg aacagttcct gacccgcggc
     1621 atcgaggcga aactgcggga acagatcggc catcctcccc atggcggcgg cgtgctcggt
     1681 ctgctgatcg aggaaccgga gccgctgatt ctcgacgacg tcagcctcca cccccgatca
     1741 cagggtttcc ctcccggcca cccggagatg aagtccttcc tcggggtccc ggtgaagatc
     1801 cgcggcgaag cctggggcaa cctctacctg accgagaagg aagagggcac tttcgacgat
     1861 gccgacatcc agtcggtggt gatcctcgcc gaatggatcg cgatcgcgat cgac
//
LOCUS       DAOQMF010000397         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_42069_length_1914_cov_3.721893, whole genome shotgun sequence.
ACCESSION   DAOQMF010000397 DAOQMF010000000
VERSION     DAOQMF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42069_length_1914_cov_3.721893"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..274
                     /locus_tag="PLV77_07030"
     CDS             29..274
                     /locus_tag="PLV77_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15636.1"
                     /translation="MDLFASVVAWKELGDTVLYAFVGAMAVSLAASLGIWGATKYVDF
                     NQDGRGVVAGMALAVGAFGLVATIGIIALGIYLMVSK"
     gene            315..665
                     /locus_tag="PLV77_07035"
     CDS             315..665
                     /locus_tag="PLV77_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15637.1"
                     /translation="MTIARNIAIIALLAAGVAFLPNGGNVADAVLTTITMAFLAGLAW
                     TVYRLTYEFRSGLLALEGSRRVILYSCFGLIVLMIAGADKMFSTGFGTMTWLLLMASG
                     LVGVWLIVSEARSY"
     gene            662..1513
                     /locus_tag="PLV77_07040"
     CDS             662..1513
                     /locus_tag="PLV77_07040"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNC15638.1"
                     /translation="MNFRVLSWNLFHGRDFPPAPGKEDPGVPPGDLLWRITGRPIPGK
                     KHVRLNRDLFHEFAALLAAAKWDIALLQEVPPRWAHRLAEATGAEARTALTSRNWLRP
                     VMSPVARFRPQLAGSWEGGCNLILVRGATPGLDKSRPGAHIREFRKSTLTWFPERRVM
                     SMVELGCGLCVANLHASTGERAAGDVMRAARLASGRAGDRPLVLGGDFNARPRSSDVF
                     DRLEGEFGLAGTTAPGSIDHLLSRGVEVLEQPEAWPPARRDVPDPETGLMVRLSDHAP
                     VLARIRV"
     gene            1583..>1914
                     /locus_tag="PLV77_07045"
     CDS             1583..>1914
                     /locus_tag="PLV77_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15639.1"
                     /translation="MDADREKAGSGGIGSRRGSVSLELIGSLPIILLAVLVAAQIAIA
                     GAALWSAGVAARAGARAVLTGNSPRGAAEHALPSVLQGGLKVSQEDGVKVGVRVPGLL
                     PGFPETHV"
BASE COUNT          265 a          612 c          683 g          354 t
ORIGIN      
        1 tgacgtgcag accgaggtgg cctgagccat ggacctgttc gcctcggtcg tcgcctggaa
       61 ggaactcggg gacacggtgc tctacgcctt cgtcggagcg atggcggtct cgctggccgc
      121 gtccctgggg atctgggggg cgaccaagta cgtcgacttc aatcaggacg ggcgcggggt
      181 ggttgcgggt atggcgctgg cggtcggggc cttcggcctg gtggcgacga tcgggatcat
      241 cgccctcggc atctacctga tggtctccaa gtagaaggaa gttccggcag cccgcggtca
      301 tttaggcttg tcgggtgact atcgccagga acatcgcgat catcgccctg ctcgcggccg
      361 gagttgcctt ccttcccaac gggggcaacg ttgccgatgc ggtgctgacc acgatcacca
      421 tggccttcct ggccggtctc gcctggacgg tctaccgact cacctatgag ttccgttcgg
      481 ggctgctggc gcttgaaggt tcgcgccggg tgatcctcta ttcctgtttc ggcctgatcg
      541 tcctgatgat cgccggtgcg gacaagatgt tttccaccgg cttcggcacg atgacctggc
      601 tgctgctgat ggcctcgggc ctggtcggcg tctggctgat cgtctcggag gctcgcagct
      661 actgaatttc cgggtactca gctggaatct cttccacggc cgcgacttcc cgccggcgcc
      721 tggcaaggaa gatcccggag ttccgccggg cgacctcctc tggcggatca cggggcgacc
      781 gatcccgggc aagaagcatg ttcgcctgaa ccgggacctc tttcacgagt ttgccgccct
      841 gctcgcggcc gcgaaatggg acatcgccct gctgcaggaa gttccgcctc gctgggccca
      901 ccgcctggcc gaagcgaccg gggccgaggc ccggaccgca ctcacttccc gcaactggtt
      961 gcggccggtc atgtcaccgg tcgccaggtt ccggccccaa ctggccggga gctgggaagg
     1021 agggtgcaac ctgatcctgg tccggggcgc gaccccgggc ctggataaat cccggcccgg
     1081 ggcccacatc agggagttcc ggaagtcgac cctgacctgg tttcctgagc gacgggtcat
     1141 gtcgatggtc gaactcggct gcggcctctg tgtggcaaac cttcacgcca gcaccggcga
     1201 aagggcggcg ggggacgtga tgcgggcggc gcggctggcc agcggcaggg cgggggatcg
     1261 ccccctggtc ctgggtggcg atttcaacgc caggccccgt tcgagcgatg ttttcgaccg
     1321 gctcgaaggc gagttcgggc tggccggaac cactgcgccg ggttcgatcg accacctgct
     1381 ttcccgcggg gtggaagtgc ttgagcagcc ggaagcctgg ccgccggcgc ggcgcgacgt
     1441 gcccgatccg gagaccggcc tgatggtcag gctctccgat cacgcaccgg tgctcgcccg
     1501 gattcgggtc tgagcacccc gacgttgcaa atcgttgcag ttgtagagcc acatgtgagc
     1561 tttttcctgc cacggtccgg gcatggatgc ggatcgcgaa aaggcaggct cgggcgggat
     1621 cggttcgcgg cggggctcgg tctcgctcga gttgatcggt tccctgccga tcatcctgct
     1681 ggccgtgctg gtcgcggccc agatcgcgat cgcgggggcg gcactctggt cggcgggggt
     1741 ggccgcccgg gccggggccc gggcggtgct caccggcaac tccccgcgcg gggcggctga
     1801 acacgcgctt ccatcggtcc tgcagggggg cctgaaggtc tcgcaggaag acggggtcaa
     1861 agtcggggtg agggtgcccg ggctgctgcc cgggttcccg gagacccatg tcta
//
LOCUS       DAOQMF010000398         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_42476_length_1905_cov_2.499459, whole genome shotgun sequence.
ACCESSION   DAOQMF010000398 DAOQMF010000000
VERSION     DAOQMF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42476_length_1905_cov_2.499459"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLV77_07050"
     CDS             <1..216
                     /locus_tag="PLV77_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15640.1"
                     /translation="KDKVTSVAEEAGEAVLPAASDARSLARDGLINLGYAPLEAEQLL
                     DGVGDGDDPQQLIGAALKRAAASGRGE"
     gene            218..1303
                     /gene="ruvB"
                     /locus_tag="PLV77_07055"
     CDS             218..1303
                     /gene="ruvB"
                     /locus_tag="PLV77_07055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNC15641.1"
                     /translation="MSDLPDFGGKRIDLGVEADLPEGAELRVHDARADQPDLELDQSL
                     RPKGLGDFVNQKQVTDQLAVFIEAARRRGEPLDHVLLAGPPGLGKTSLANIVAAEMGV
                     PLIQTAGPALERKGDVAAFLTSLEPGSVFFIDEIHRLGRAVEETLYPAMEDGELPVVL
                     GQGAGAQTVTLPLPPFTLIGATTRSGLLTTPLRDRFGVSHRLEYYTPADLARIVIRSA
                     GILGVEIDEGGAVAIAERSRGTPRVANRLLKRVRDFAEVKGSGSVDAEMADAALEMLE
                     VDKAGLDRADRALLELIATRFQGGPVGLSTIAVAIGEEQDTIEDVLEPYLLQMGMLKR
                     TPRGRVLTQSAFEHLGLPVPSGHQSLF"
     gene            complement(1300..>1905)
                     /locus_tag="PLV77_07060"
     CDS             complement(1300..>1905)
                     /locus_tag="PLV77_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC15642.1"
                     /translation="LIEATAAAGAPRVVTQSISFLYELSGPMIKAEEEPVDRSGVHDA
                     VLGHERKSLEDDRFEAVVLRYGLLYGPGTWYARDGYLSGEVRKRRLPIVGGGHGMTSF
                     LHVEDAASAAVAALEKGEGIYNVTDDEPAATRDWIPVFAESVGAKPPRKVPFWLAALI
                     AGKPIALQATEGRGASNAKFKAQTGWEPSIPSWRQGFFEAT"
BASE COUNT          295 a          636 c          653 g          321 t
ORIGIN      
        1 aaggacaagg tgacttcggt cgccgaggaa gcgggagaag cagttcttcc cgccgcctcg
       61 gacgcccgct cgctcgctcg cgacggtctg atcaatctcg gttacgcccc gctggaagcc
      121 gagcagctgc tcgacggcgt cggcgatggc gatgacccgc agcagctgat cggcgcggcc
      181 ctcaaacgcg cagccgcgtc cggccggggg gagtgagatg agcgacctgc ccgatttcgg
      241 tggcaagcgg atcgacctcg gggtcgaggc cgacctgccc gaaggtgccg agctgcgggt
      301 ccacgacgcc cgggccgatc agcccgacct cgagctcgac cagtcgctca ggccgaaagg
      361 gctcggggat ttcgtcaacc agaagcaggt gaccgaccag ctggccgtgt tcatcgaggc
      421 tgcccgccgc cggggggaac cactcgatca cgtgcttctg gccggtccgc ccggtctggg
      481 caagacctcg ctggccaaca tcgtcgccgc cgagatgggc gtgccgctga tccagaccgc
      541 cggcccggcc ctggaacgca agggtgacgt cgccgccttt ctcacttcgc tggagcccgg
      601 cagtgtcttc ttcatcgatg agattcaccg gcttgggcgg gccgtggaag agactctcta
      661 cccggcgatg gaagacggtg agctgccggt ggtgctcggc cagggggccg gcgcccagac
      721 cgtgaccctg ccgttgccgc cgtttaccct gatcggggcc actacccgca gcggcctgct
      781 gacgactccg ctgcgggacc ggtttggcgt ctcccatagg ctcgagtact acacaccggc
      841 cgatctggcc aggatcgtga ttcgttcggc cgggatcctc ggggtcgaga tcgacgaggg
      901 gggagcggtg gcgatcgccg agcgttcccg cggcacgcca cgggtcgcca accgcctgct
      961 caaacgggtg cgggacttcg ccgaggtcaa gggctccggt tcggtcgatg cggaaatggc
     1021 cgacgctgcg ctcgagatgc tcgaagtcga caaggccggc ctcgaccggg ccgaccgcgc
     1081 cctgctcgag ttgatcgcga cgcggtttca gggcgggccg gtcggccttt cgacgattgc
     1141 ggtggcgatc ggtgaggagc aggacacgat cgaagacgtg ctggagccct atctgctcca
     1201 gatgggcatg ctgaagcgca ccccgcgggg gcgggttctt acgcagtcag ctttcgagca
     1261 cctcggcctg ccggttccct cgggccatca gtcgctcttc taggtcgctt cgaaaaatcc
     1321 ctggcgccaa ctggggatcg acggttccca tccggtctgc gccttgaact tcgcgttcga
     1381 tgcgccccgg ccctcggtgg cctgcaacgc gatcggtttg ccggcgatca gcgcggccag
     1441 ccagaacgga accttgcgtg gtggtttggc cccgaccgac tctgcgaaaa cgggaatcca
     1501 gtccctggtc gcggccggtt catcatcggt cacgttgtag atcccctcgc ctttttccag
     1561 ggcagcgacc gcggctgaag ccgcatcttc gacgtggagg aaagaggtca tgccgtggcc
     1621 gccaccgacg atcggcagcc gccgcttgcg gacttcaccg gacaggtagc cgtcacgggc
     1681 gtaccaggtg cccgggccgt agagcagccc gtaacggagc acgaccgcct cgaaccggtc
     1741 gtcttcgagc gactttcgct cgtggccaag cacggcgtcg tggaccccgc tgcgatcaac
     1801 cggttcctcc tcggccttga tcatcggtcc gctcagctcg tagaggaagg agatgctctg
     1861 ggtgaccacg cggggggcgc cggcggcggc cgtcgcttcg atcag
//
LOCUS       DAOQMF010000399         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_42510_length_1904_cov_3.359113, whole genome shotgun sequence.
ACCESSION   DAOQMF010000399 DAOQMF010000000
VERSION     DAOQMF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42510_length_1904_cov_3.359113"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="rsmI"
                     /locus_tag="PLV77_07065"
     CDS             complement(<1..781)
                     /gene="rsmI"
                     /locus_tag="PLV77_07065"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNC15643.1"
                     /translation="MPGRLTVCPTPIGNMDDVSKRVREALVMADYVACEDTRRTGRLL
                     ERLEIRPKPRLVSNHEGNEASRAVELVKRMERGDKIALVSDAGMPAVSDPGYRLIRAC
                     IDRGIEVEVLPGPSVIPVALVASGLPTDRWRFEGFLPKRAGEMERVLNSAETVIAFES
                     PRRISNSLRALSQLAPDRQAAVCREMTKLHEEVSRGTLTELAMRFQGEVKGEIVLVIG
                     PAAAAEHGQDIAFAVEALKRLVKSGARPRAAEPSRRSERVFV"
     gene            complement(781..1737)
                     /locus_tag="PLV77_07070"
     CDS             complement(781..1737)
                     /locus_tag="PLV77_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC15644.1"
                     /translation="MAEVESIGADLGGTKLAVGVVDGRPEALWQEEVPSRGYSQERVV
                     NLLAQQINKAHQARPTASSVGVGIPATLDHETGHAISTVNLDLEDLPIREMLSRETRL
                     PVAIDNDGNLAMLAEALYGAVRGTSNALLLTIGTGIGGGIWLNGEIYRGSTGAGAELG
                     HLVVEIDGHPCQGNCPNRGCVEAMASGTAIGRHGREAAEREPDSLLGRRLAAGEEIDA
                     RAVNEAATAGDQAAIGAIDLAGHYLGQALVGLSNVFQPEVIVLGGGAIAFGELLIAPA
                     RREVEKFALKPMNETKVVAAELGPSAGMIGAAAYGRMEAEAA"
BASE COUNT          295 a          672 c          614 g          323 t
ORIGIN      
        1 acacgaagac gcgctcgctc cgacggctgg gctcggccgc cctcggcctg gccccggact
       61 tgaccaggcg cttcagcgct tccacggcga aggcgatgtc ctggccgtgc tcggccgccg
      121 ctgccggacc gattaccagg acgatctccc ccttgacctc gccctggaac cgcatcgcca
      181 gctcggtcag ggtgccgcgg gagacttcct cgtggagctt ggtcatctcg cggcagaccg
      241 cggcctggcg gtccggggcg agctgggaga gggcccggag cgagttcgag atgcgtcgcg
      301 gtgattcgaa ggcgatcacc gtctcggccg aattcagcac ccgttccatc tcgcccgccc
      361 gcttcggcaa aaatccctcg aaccgccagc ggtcggtcgg cagaccggat gcgaccagag
      421 cgaccggaat caccgaaggg ccgggcaaca cctcgacttc gatgccccgg tcgatgcagg
      481 cccggatcag ccggtaaccg ggatcggaga ccgcgggcat gccggcgtcg gagacgaggg
      541 cgatcttgtc accccgttcc atccgcttga ccagttccac cgcccgtgag gcctcgttgc
      601 cctcgtgatt ggaaaccagg cgcggtttgg ggcggatctc gagccgttcg agcagccggc
      661 cggtacgtcg cgtgtcctcg caggccacat agtcagccat caccagggcc tcgcggaccc
      721 gcttgctgac gtcatccatg ttgccgatcg gggtcgggca aacggtgagg cggccgggca
      781 tcaggcggcc tcggcttcca tgcgaccgta ggcggcagcg ccgatcatgc cggcggacgg
      841 gccgagctcg gcggccacga cctttgtctc gttcatcggt ttcagggcga acttttcgac
      901 ttcccgccgg gccggggcga tcaacagttc gccgaaggca atcgcacctc cgcccagcac
      961 gatcacctcg ggctgaaaga cgttcgacag gccgaccagg gcctggccga ggtaatggcc
     1021 ggccaggtcg atcgcgccga tcgccgcctg gtcaccagcg gttgcggcct cgttgactgc
     1081 cctggcgtcg atttcctcgc cggccgcgag gcgccgccca agcagcgagt caggttcccg
     1141 ctcggcggct tcgcgaccgt gcctgccgat cgccgtgccg gaggccatcg cctcgacgca
     1201 accgcggttc gggcagttgc cctggcaggg atgcccgtcg atttcgacca ccagatggcc
     1261 gagttccgct ccggcgccgg tcgatccccg gtagatctcg ccgttcagcc agatcccgcc
     1321 gccgatcccg gtgccgatcg tgagcagcag ggcattgctg gtcccgcgta cggccccgta
     1381 gagcgcttcg gccagcatcg ccaggttgcc gtcgttgtcg atcgccaccg gcagccgggt
     1441 ctcgcgggac agcatctcgc ggatcggcag gtcttcgagg tcgaggttga cggtgctgat
     1501 cgcgtgaccg gtctcgtgat cgagcgtcgc gggtatcccg accccgaccg aggaagcggt
     1561 cggacgggcc tgatgggcct tgttgatctg ctgcgccagc aggttcacga ccctctcctg
     1621 cgaataacct ctggagggga cctcctcctg ccacagggct tcgggccgcc catcgaccac
     1681 gccgacggca agctttgtcc cacccaggtc agctccgatc gattcaactt ctgccatcag
     1741 ccaccatttc taactcttca tgccttcctc aggtaacaag cccgatccgg accggcccga
     1801 ctacaacgtc tatcgctcgc gccggggcct cttttcccgc ttccgcaagc ctgatctcga
     1861 atccctgagc gagaaggaga agaaggcggc ggaccgtcaa aggg
//
LOCUS       DAOQMF010000400         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_42602_length_1902_cov_3.199242, whole genome shotgun sequence.
ACCESSION   DAOQMF010000400 DAOQMF010000000
VERSION     DAOQMF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42602_length_1902_cov_3.199242"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLV77_07075"
     CDS             complement(<1..183)
                     /locus_tag="PLV77_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15645.1"
                     /translation="MNGAGSFRLAVAQPAIDREAPESSRVAEAGAMIREAGRGEADLI
                     LFPESFPGPITAGLDYD"
     gene            complement(180..767)
                     /locus_tag="PLV77_07080"
     CDS             complement(180..767)
                     /locus_tag="PLV77_07080"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HNC15646.1"
                     /translation="MAMAEITTEVPHGTARRFDLRTGDLIRIETLDGAQGGDFSFPGF
                     DQALTRNKLGWKRFGRPWLVYDAQAGDSLFDVDGEATFELIALEGEGVADIMYPGCWD
                     EVYEDRRPGCQDLISAALTIPRRDLAGMLSFFIGYSADNSAYRGLAGIDLKPGDHLTF
                     RALRDVSCAVSACPDRDIPGWRVGALFVSVTEAGK"
     gene            complement(851..1531)
                     /locus_tag="PLV77_07085"
     CDS             complement(851..1531)
                     /locus_tag="PLV77_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNC15647.1"
                     /translation="MKISRSSLIAAAVLVTMAVAAPAGAPADPAPRAGEGFQGRIFRI
                     DRDLRERMVGSSWHRGCPVRLADLRLLRLSYRDFDGDVRRGYLVVHETAAPRMVKAFG
                     RLFNAGYRIRRMRLVDAYGADDHRSMNADNTSAFNCREVAGQPGVWSRHAYGRAIDIN
                     PVENPYVSGSHVSPARGRPFADRYPRRKGMISAGGRVVRIFAAIGWGWGGDWAGAKDY
                     QHFSASGD"
     gene            1632..>1902
                     /locus_tag="PLV77_07090"
     CDS             1632..>1902
                     /locus_tag="PLV77_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15648.1"
                     /translation="MKKSVAIGIAGVTAVVVAAWRQVQRDNTLATDAGRAPVPARPPA
                     VRNDAAPSTPAPSSSPDALFTPSVSASSTKAELYEVATELGIEGRS"
BASE COUNT          328 a          654 c          619 g          301 t
ORIGIN      
        1 gtcgtaatcg aggccggcgg tgatcggtcc ggggaagctc tccgggaaga ggatcaggtc
       61 agcctcgcca cgaccggctt cccggatcat cgccccagcc tctgccaccc gggaggattc
      121 gggagcttcg cggtcgatcg ccggctgggc gaccgccagg cggaagctgc ccgccccgtt
      181 cacttgccgg cctcggtgac cgatacgaac agggcgccga ccctccagcc ggggatgtcc
      241 cggtcggggc aggccgaaac cgcgcagctg acgtcgcgca gcgcccggaa ggtcaggtgg
      301 tcgccgggct tcaggtcgat gccggccaga ccccgataag cggagttgtc ggccgagtag
      361 ccgatgaaga aggagagcat gccggccagg tcccgccggg gaatcgtgag cgccgccgag
      421 atcaggtcct ggcaacccgg tcgccggtcc tcgtagacct cgtcccagca acccggatac
      481 atgatgtcgg cgactccctc cccttcgagg gcgatcagtt cgaaggtcgc ctcgccgtcg
      541 acgtcgaaga gcgaatcacc ggcctgggcg tcgtagacca gccagggccg gccaaaccgc
      601 ttccagccga gcttgttccg ggtcagggcc tgatcgaaac cggggaagct gaagtcgcct
      661 ccctgcgcgc cgtccagggt ctcgatccgg atcagatccc cggtccgcaa atcgaacctc
      721 cgggcggtcc cgtgaggcac ttcggttgtg atctccgcca ttgccatcgc acccgctttc
      781 agtcgggtcg cactccttta tcgaagcggt gcttacgaac tcagtgcgac ggtgtaaagg
      841 gattttagcc ctagtcgccg gaggcggaga agtgctggta atccttggcg ccggcccagt
      901 cgccgcccca tccccagccg atcgcggcga agatccggac gaccctgccg ccggcactga
      961 tcattccctt gcggcggggg tagcggtcag cgaaaggccg gcccctggcc ggagaaacgt
     1021 ggctccctga cacatacggg ttctcgaccg gattgatgtc gatcgcccgg ccgtaggcat
     1081 gccgggacca gaccccgggc tggccggcga cctcgcggca gttgaaagcc gaggtgttgt
     1141 cggcgttcat cgagcggtgg tcatcggcgc cgtaggcatc gaccaggcgc atgcgacgga
     1201 tccggtagcc ggcgttgaag agacggccaa aagccttgac catgcggggc gcggccgtct
     1261 cgtggacgac caggtatcca cgccggacgt cgccgtcgaa atcccggtag ctcagcctga
     1321 gcagccgcag gtcggcaagc cggaccgggc atccccgatg ccaggaggaa ccgaccatgc
     1381 gttctctcag atcccggtcg atcctgaaga tcctgccctg gaacccttcc cccgcccggg
     1441 gcgccggatc ggcgggggca cccgcaggcg ccgcaacggc catcgtcacc aggacggccg
     1501 cagcgatcag gctcgatcgg gaaatcttca cgattcgaga ctagacaatc gtccagaagt
     1561 ccaaaccgct tcgtcggtag agtcctgcct gtgatcaatt gcttcccttc cagtctcaaa
     1621 tggtcgaagt catgaagaag tccgtcgcga tcggcattgc cggcgtgacc gcggtcgttg
     1681 tcgccgcctg gcgtcaggtc cagcgggaca acactcttgc caccgatgcc ggtcgagctc
     1741 cggtgcccgc ccggcccccg gcggtcagaa acgatgcggc tccgtccacc ccggccccga
     1801 gcagctcccc cgacgccctc tttaccccgt cggtcagcgc cagctcgacc aaggccgagc
     1861 tttacgaggt cgcaacggaa ctgggcatcg agggccgctc ga
//
LOCUS       DAOQMF010000401         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_43077_length_1891_cov_2.929739, whole genome shotgun sequence.
ACCESSION   DAOQMF010000401 DAOQMF010000000
VERSION     DAOQMF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43077_length_1891_cov_2.929739"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLV77_07095"
     CDS             complement(<1..732)
                     /locus_tag="PLV77_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15649.1"
                     /translation="MDRPEPQTEEEFEREFRRAGLPLLNENYSASEDIFNRAVPLLGL
                     VLVLEVTGPLNKSWSTLGNVAAVATAMLLMLVALGLMNRMRGRAFFSLPRKVGRPELA
                     AFVIVPALLPGIISGHWGIAALTALFNLALLLVILGVVGFGVISIIHWALARLLGELS
                     RSLGLLVKAVPLLMIFSLILFLTPELWQVFATLPDQSLLLLVALFMLLGSAFLIARIP
                     HEVASLEKEAGGGGPPLRQRQRFNVG"
     gene            773..1309
                     /locus_tag="PLV77_07100"
     CDS             773..1309
                     /locus_tag="PLV77_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC15650.1"
                     /translation="MKQLDLKLLRIMRTRGHAAPVEKSAHLISKSGENGYLWFGIGLA
                     GAVFDGEHRKEWLLASLLGPVAIGLNFLVKLVVRRPRPELEGLPPLGGAPSSLSFPSA
                     HATASFAAATAMSRIRPELKLALFGAAAVMAVSRPYLGMHYPSDIAAGAVLGTVLGTA
                     AGNFLAGPEEDENSPYNF"
     gene            complement(1395..>1891)
                     /locus_tag="PLV77_07105"
     CDS             complement(1395..>1891)
                     /locus_tag="PLV77_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNC15651.1"
                     /translation="LSSKGTHDGFDVYPDTRSQVYGGISTEYPRTNRACAKTPSQALF
                     YGGKVAMALFSASSGGHTESIQNMWGGAAIPYLQGVADPWDKVSPYYRWTYSFSPARM
                     NSLLGSYLAGRLRSVKITQTGVSKRVMWARLYGTRGVTKIRGDSLQYALGLPDRLIFS
                     IRKK"
BASE COUNT          346 a          603 c          629 g          313 t
ORIGIN      
        1 cccgacgttg aaccgctgcc gctgcctgag cggcgggcct cccccgcccg cctccttttc
       61 gaggctggcc acctcatggg gaatccgggc gatcaggaag gccgagccga gcaacatgaa
      121 gagggcgacc agcagcagga gcgactgatc gggcagggtc gcgaagacct gccagagctc
      181 cggggtcagg aagaggatca gagagaagat catcaagagc ggcactgcct tgaccagcag
      241 cccgagcgaa cgggaaagct ccccgagcag gcgggccagg gcccagtgga tgatcgagat
      301 cacgccgaaa ccgacgactc cgaggatgac gaggagcagg gcaaggttga agagggccgt
      361 cagggccgcg attccccagt ggccggaaat gattcccggc aggagggccg gcacgattac
      421 gaaggcggcg agctcggggc gtcccacttt gcgcggcagc gagaagaagg cgcggccgcg
      481 catccggttc atcaggccga gagcgaccag catcaacagc atcgcggtcg ccaccgcggc
      541 gacgttgcca agggtcgacc agctcttgtt cagaggtccg gtcacttcca ggaccaggac
      601 cagtccaagc agcggcacgg cccggttgaa gatgtcctcc gaagccgagt aattctcgtt
      661 caggagcggc aggccggcgc ggcggaattc ccgctcgaac tcttcttcag tctggggttc
      721 gggacgatcc acgccgcgat catcccgaat ggccccctat tctgctcact tcgtgaagca
      781 actggatttg aaactgctgc gcatcatgcg cacccgcggt cacgccgctc cggtcgagaa
      841 gtcggctcac ctgatctcga agtcgggcga gaacggctac ctctggttcg ggatcggcct
      901 ggccggcgcg gtttttgacg gagagcaccg caaggagtgg ctgcttgctt cgctgctcgg
      961 cccggtggcg atcggcctta acttcctggt caagctggtg gtaaggcgtc cgcggccgga
     1021 gcttgaaggc ctgcccccgc tgggcggtgc gccctcttcg ctttcgttcc cctccgccca
     1081 tgccacggct tcgtttgccg cggctacggc gatgagccgg atccgcccgg aattgaaact
     1141 cgccctcttc ggagccgccg ccgtgatggc ggtcagccgt ccctacctcg gcatgcacta
     1201 ccccagcgac atcgccgctg gggcggttct tggcacagtc ctcggaaccg ccgccggaaa
     1261 cttcctcgcc ggccccgaag aagacgaaaa ctcgccttac aacttctagc cacggatcac
     1321 cgaatgccca ctttgtgccg gtatgtggcg caaagtggga tgtcgatgaa tccgaggcgt
     1381 gaccgcccgg gtcgctactt cttcctgatc gagaagatca gtcggtcggg gagtccgagg
     1441 gcgtactgga gggaatcgcc cctgatcttc gtaacgcccc gggtgccgta gaggcgggcc
     1501 cacatgaccc gcttcgaaac cccggtctgg gtgatcttca ccgagcggag gcgcccggcc
     1561 aggtaactgc cgagcaggga gttcatccgg gccggcgaga agctgtaggt ccagcggtag
     1621 tacggcgaaa ccttgtccca ggggtcggcg acgccctgca ggtacgggat cgccgcgccg
     1681 ccccacatgt tctggatgct ctcggtgtga ccaccggaag atgccgagaa gagggccatc
     1741 gcaaccttgc cgccgtaaaa gagggcctgg ctcggagtct tcgcgcaggc ccggttggtc
     1801 cgcgggtact cggtcgagat cccgccgtag acctggctgc gggtgtcggg atagacgtcg
     1861 aatccgtcat gggttccctt cgaggaaagg c
//
LOCUS       DAOQMF010000402         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_43197_length_1888_cov_3.115112, whole genome shotgun sequence.
ACCESSION   DAOQMF010000402 DAOQMF010000000
VERSION     DAOQMF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43197_length_1888_cov_3.115112"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLV77_07110"
     CDS             <1..287
                     /locus_tag="PLV77_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15652.1"
                     /translation="ICCEGNTVIECVGEFWRKFVDFASALEAGAVDLRWLPDTDKREM
                     YAGVMADRFLAAIATPPSDGDPYELRGEFWDGKCPREKNPYQKILSYPPF"
     gene            388..1269
                     /locus_tag="PLV77_07115"
     CDS             388..1269
                     /locus_tag="PLV77_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15653.1"
                     /translation="MSESEEIDRPDFLGESSLILEVMFNELTGAGRSSATIDLYLRVA
                     KRFEDHLMKKDPPVFADELELDEAHQLVKDWFQLELSPTVSKPTAATYRTIMATYLNA
                     LDGTFGRENLVFRLGVEANKRQKDTWAGRPHPSEPLDTRQLARIRQASAQKTGKLWIR
                     TAIELALAGLTIPEIMKVRAHHVSLFGQPQIRCLERSVPILDPETTGWIDFLAKVTAE
                     RKYPRDAVSERTVKRVLTREVAAVRKNRPYGSHQGIRTLHNTGARQMLRAGRKPERVR
                     KIYGRANFPESWNRRST"
     gene            1634..>1888
                     /locus_tag="PLV77_07120"
     CDS             1634..>1888
                     /locus_tag="PLV77_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15654.1"
                     /translation="MKLETANQGMTSRRQIALAVGLGILMMIGALISSAGIAKANDSS
                     YPVRLCSPERFDGNGYPYGTFNSSLGTGTRVFYKAANGNIT"
BASE COUNT          442 a          533 c          579 g          334 t
ORIGIN      
        1 ggatctgctg cgaagggaac accgtgatcg aatgcgtagg cgagttctgg aggaagttcg
       61 tcgacttcgc aagtgccttg gaggccggtg cggttgatct cagatggctt cccgacacgg
      121 acaaacggga aatgtacgcc ggcgtgatgg ccgaccgctt cctggcggcg atcgcgaccc
      181 cgccaagcga cggtgatccc tacgagctga ggggtgagtt ttgggacggc aagtgccccc
      241 gcgaaaagaa cccgtaccag aagattctgt cgtatccgcc attctaggac gggacaccta
      301 gcgtgcagat cctagaaccg gcttgtgatg cggtaaaccc ttgatcaggc accgttttaa
      361 gtgccatcta gcaggaaggg ttttgctgtg agcgaatccg aagaaataga ccgtcccgac
      421 tttcttggtg aaagctccct gattctcgag gtgatgttca acgagctgac cggtgccgga
      481 agatcctcag cgacaataga cctgtatctc agggtcgcca aacgcttcga ggatcacctg
      541 atgaaaaagg atccgccggt ctttgcagac gagcttgaac tcgacgaagc ccaccaactg
      601 gtcaaggact ggtttcagct ggagctctcg ccgacggtgt cgaaaccgac ggcggccacc
      661 tacaggacga tcatggctac ctacctgaac gcgcttgacg gtaccttcgg ccgggagaac
      721 ctggtgttcc ggctcggtgt ggaagccaac aaacgccaga aagacacctg ggcgggaaga
      781 ccccatccga gcgagccgct cgacacgaga cagctcgccc gtatccggca ggcgagtgcc
      841 caaaagaccg ggaagctctg gatccgaact gcgatcgagc ttgccttggc cggcctgacg
      901 atcccggaga tcatgaaggt gcgcgcacat cacgtcagcc ttttcgggca gccgcagatc
      961 agatgccttg agcggagcgt gccgatcctg gatcccgaaa caaccggatg gattgacttt
     1021 ctcgccaagg tcacggccga gaggaaatac ccgagagatg cggtcagcga gcgaaccgtg
     1081 aaaagggtct tgaccagaga ggtagccgcg gtccgcaaga acaggcctta cggcagtcac
     1141 caggggatta gaacccttca caacacaggg gcaagacaga tgctccgggc cggcagaaag
     1201 cccgaaagag tcagaaagat atacggacgc gccaacttcc cggaaagctg gaatcgccgc
     1261 tccacatagg gctttgagcg ttctggaggt tgccgccagg aacgcttccg gagaataaaa
     1321 ggggataggg gggggtgggt tttcgggtcg ctgcggagac ctaccccata tgaaggtcaa
     1381 acgcggcacc tcaaaagcag gactggcgag ctgggcggcg aataccgtct gtttcagttc
     1441 gttgacacgc aacttcttcg ctcgtagcat gttttacggc gcgaatgggc ccgaggcacg
     1501 aatctccggc agttcgacct tcagcgatcg gtccctgaga ccccgaggga gctaccaggc
     1561 tcagggagag gacgggaccg atcgcattca acaaagctat ggggagggag tccccgggaa
     1621 tgaaggagta gttgtgaaac tcgaaactgc aaaccagggg atgacctcac ggcgtcaaat
     1681 cgcgctggcc gtgggactcg gcatcctgat gatgatcggg gctctcatct cgagcgccgg
     1741 aatcgcgaaa gccaacgaca gttcctaccc ggtacgcctt tgctcgccgg aacgcttcga
     1801 cgggaacggg tacccatacg gcactttcaa ctcttccctc ggtaccggaa cccgtgtctt
     1861 ctacaaggcc gcgaacggca acatcacc
//
LOCUS       DAOQMF010000403         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_43233_length_1887_cov_3.104803, whole genome shotgun sequence.
ACCESSION   DAOQMF010000403 DAOQMF010000000
VERSION     DAOQMF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43233_length_1887_cov_3.104803"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..931)
                     /locus_tag="PLV77_07125"
     CDS             complement(173..931)
                     /locus_tag="PLV77_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNC15655.1"
                     /translation="MDVLAVSDPAAGVGLVAALFAGMVSFLSPCVLPLVPGYLSAVSG
                     VSVDELEDAGWRRVLLPSLCFVASFSLIFILLGLTATKLGSILSDNRQLLDKIAAALI
                     IAMGVLFVASFFVVKLNREWHVDALMSRAGKGGPVVAGAAFAFAWTPCIGPTLGAILS
                     AASLSGSAAKGALLLAVYSAGLAVPFLLTVLAFDRMTSAFSVVKRHYELIVAAGGLIL
                     IAMGILIWTGELFRINIEMQKWMSQVGLDFWSSI"
     gene            complement(960..>1887)
                     /locus_tag="PLV77_07130"
     CDS             complement(960..>1887)
                     /locus_tag="PLV77_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC15656.1"
                     /translation="FTLAAAVQQGMDPDNTYYESKPLSIDDPVYGPWEVATYGDTYAG
                     TTSVAEATLASDNSVYAQMALDIGPKEVAEIAKKMGVETKLDGFPAETLGGLRIGVSP
                     LEMADAYATLASGGIHRDPVVIEKVVFPGGTEERPGRSDPERVLTEPEAAVVTDILRR
                     NITEGTGTGAYTGCEGQAGKTGTTDNFVDAWFIGYQPNLATATWVGYPESNNISMPGV
                     AGGTLPASIWNQFFTNAEVPCEEFPISEEQIDWADTEFEYSVSGEEADPDQGLDTPGE
                     AETLPSEVPTAPEPELPSIDQGGITGGVSPDG"
BASE COUNT          404 a          588 c          541 g          354 t
ORIGIN      
        1 ccggttcgga tcggccaacg ccttcgcgaa cgaagcccca ctttcgagaa cttcagcgtc
       61 gggaatagac gaccgaacac tttcagcctt cgggaggtcg agacaaagca gttcgcacgc
      121 atctgttccg ggcttcattc caacatccta atacatgtgc gaataatcgt agttagatgc
      181 tgctccagaa atcgagaccg acctgactca tccacttctg catctcgatg ttgatgcgga
      241 acagttcacc cgtccagatc aggatcccca tagcgatcag gatgagaccg cccgcggcaa
      301 cgatcagttc gtagtgccgc ttgaccacac tgaaggccga agtcattcgg tcgaaggcca
      361 agaccgtaag gaggaagggg actgccaggc ctgccgagta gaccgcgaga agcagtgccc
      421 ctttcgcggc agatcccgac aatgaagcag ccgacagaat cgcccccagc gtcggcccga
      481 tgcatggagt ccaggcaaag gcgaaggcgg cgccggccac gactggccct cccttgcccg
      541 cgcgagacat caatgcgtct acatgccact cccggttcag cttgaccaca aagaaggatg
      601 cgacgaacaa gacccccatc gcaatgatca acgccgccgc gatcttgtcg agaagctggc
      661 ggttgtcgct gagaatcgac ccgagtttcg ttgccgtgag cccaagcaag atgaagatca
      721 gggagaagct cgcgacgaag cagaggctcg gcaagaggac ccgacgccac cccgcatcct
      781 cgagttcgtc caccgacaca cccgagaccg ccgacaaata gcccgggacc agagggagaa
      841 cgcaagggga caggaacgag accatcccgg caaaaagggc agctacgagt cccaccccgg
      901 cggccgggtc agatacggca agcacatcca tctactacaa acgttagtag gtcttggcct
      961 cagccgtccg gggatacccc gccagtgatc ccaccttggt caatgctcgg cagctccggt
     1021 tcgggagctg tcggaacctc ggaaggaaga gtctcagctt cgcccggagt atccaggccc
     1081 tggtcagggt cggcctcctc gccggagact gagtattcga actcggtgtc ggcccagtcg
     1141 atctgttctt cagagatcgg aaattcctcg catggaacct ccgcattggt gaagaactga
     1201 ttccagatgc tggcgggaag cgtgcctccg gctacccccg gcatgctgat gttgttcgac
     1261 tccgggtagc caacccacgt ggcggtggcg agattgggct ggtacccgat gaaccatgcg
     1321 tcaacgaaat tgtcggttgt gccagtcttc ccggcttgac cttcacagcc cgtataggca
     1381 ccggtaccgg tgccttccgt gatgttgcgg cgaaggatgt ccgtaacaac ggctgcttcc
     1441 ggttcggtca acactcgctc gggatcgctg cgcccaggac gttcttcggt tccgcccgga
     1501 aagacgactt tctcgatcac cacgggatcc cggtgaatgc ctccggaagc gagtgtggcg
     1561 taagcatctg ccatctcgag cggtgacacg cctatccgaa gaccgcccag agtctctgcc
     1621 gggaatccgt cgagctttgt ttcgaccccc atcttcttcg cgatctccgc tacttccttt
     1681 gggccgatgt ccaaagccat ctgagcgtag accgaattgt ccgaagcgag cgttgcctcg
     1741 gcaacgctcg tggttcccgc ataggtgtct ccgtaagtcg ccacctccca cggcccgtag
     1801 accggatcgt ctatcgagag tggcttggat tcgtaatagg tgttgtccgg gtccatgcct
     1861 tgctgcaccg cggcggcgag cgtgaat
//
LOCUS       DAOQMF010000404         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_43266_length_1886_cov_4.598580, whole genome shotgun sequence.
ACCESSION   DAOQMF010000404 DAOQMF010000000
VERSION     DAOQMF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43266_length_1886_cov_4.598580"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PLV77_07135"
     CDS             complement(<1..976)
                     /locus_tag="PLV77_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNC15657.1"
                     /translation="MDELTDKQPAAGPVAEEAPGHHHHHHHDEVDLNPAGWRDGKRYA
                     WLLGILVPLLPFISWQLVEATGLGIFWYSAPLVVFGLFPLLDTVIGLDPTNPPDSVLK
                     WLEQDRYYRWCTYMFIPVQYAGLVFACWQWADGGLSVFESIGLTLTMGVVGGIAINTA
                     HELGHKREAMERWLGRVALAQTGYGHFFIEHNRGHHVRVATPEDPASSRMGESFWAFL
                     PRTVSGSLRSAWGIEAARLDRLGVSHWSYKNDILSAWAMTVLLFGGLAVAFGPVVLPY
                     LIIQAVVGFSLLEVVNYLEHYGLLRQRKDDGRYERCQQQHSWNSNNVAS"
     gene            1066..1599
                     /locus_tag="PLV77_07140"
     CDS             1066..1599
                     /locus_tag="PLV77_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15658.1"
                     /translation="MAVGEARPGYELWRPNREKAECLTTRFIAVVLLVASAALMLIVT
                     LAGWDVLVGGQTYGIISLIFVGVYILLAVMIWRWSRGALTLTMALSVLLGIFCAVGAP
                     TWFARDKAGFTEPGLPSDLIGLLVLLLIPVQIAVLLTCAIGFRQEWHVEEERPISSGD
                     ADAGGDQVESGSLAGAS"
     gene            1628..1711
                     /locus_tag="PLV77_07145"
     tRNA            1628..1711
                     /locus_tag="PLV77_07145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1662..1664,aa:Leu,seq:caa)
BASE COUNT          354 a          564 c          637 g          331 t
ORIGIN      
        1 tggaagcgac gttgttgctg ttccagctgt gctgctgctg gcacctttcg tatcgaccgt
       61 catccttgcg ctgtcgcagc aggccgtagt gctccaggta attgacgacc tcgagcaggg
      121 agaagccgac cacggcctgg atgatcagat agggcaggac gaccggcccg aaggcgaccg
      181 cgagaccgcc gaagaggaga acggtcatcg cccacgcgct gaggatgtcg ttcttgtaac
      241 tccaatggga aacacccagc cggtccagcc gtgcggcttc gatcccccag gccgagcgca
      301 ggctgcccga gacggttcgg ggcaaaaagg cccagaagct ctcgcccatc cgcgagctgg
      361 ccgggtcctc gggcgtcgca acgcggacgt gatgaccgcg gttgtgttcg atgaagaaat
      421 ggccatagcc ggtctgggcg agcgcgaccc ggcccagcca gcgttccatc gcttcgcgct
      481 tgtgaccgag ctcgtgggcc gtgttgatgg caattccgcc aaccacgccc atggtcaggg
      541 tcagtccgat cgattcgaaa accgagaggc cgccatcggc ccactgccag caggcaaaga
      601 ccagcccggc gtactggacc gggatgaaca tgtaggtgca ccagcggtag taacggtcct
      661 gctccagcca tttgaggacg ctgtcggggg gattggtcgg gtcgagaccg atcacggtgt
      721 cgagcagggg aaacagcccg aagacgacca gaggcgccga gtaccagaag atccccagtc
      781 cggtcgcttc gaccagttgc caggaaatga agggcaagag ggggacgagg atgccgagca
      841 gccaggcgta gcgcttgccg tcgcgccagc cggccgggtt tagatcgact tcatcgtggt
      901 gatggtggtg atgatgaccc ggcgcttctt cagcgaccgg tcccgcagca ggttgcttgt
      961 cagtaagttc gtccatgtcg ctttacttta cacaaaattg ctttttgtaa acatcagacc
     1021 ttgatccggg ctgcggacat ccgggtttga atctatgatc ggctcatggc ggtaggagag
     1081 gcaaggcccg gttacgagct gtggcgcccg aaccgggaga aagcggagtg cctgaccacg
     1141 cggttcatcg cggtggtgct gctggtcgca tcggcagcct tgatgctgat cgtgactctc
     1201 gccggatggg acgtcctggt cggcgggcag acctacggga tcatctccct gatcttcgtc
     1261 ggggtttaca tcctgctggc ggtaatgatc tggcgctgga gccgcggcgc gctcacactg
     1321 accatggccc tttcggttct gctggggatc ttctgcgcgg tcggagctcc gacctggttt
     1381 gcccgggaca aggccggctt caccgaaccg ggtctgccct ccgatctgat tggcctgctg
     1441 gttctcctgc tcatcccggt acagatcgca gtgcttctga cctgcgcgat cggattccgg
     1501 caggaatggc acgtcgagga agagcgcccg atcagctccg gggatgcgga cgcgggcggc
     1561 gaccaggtcg agtccggctc actggccggc gcctcctgag ccgagcggca taaacttgag
     1621 cgtcccggcg gcggtggcgg aactggtata cgcgacggac tcaaaatccg ttgcccgcaa
     1681 gggcgtgtgg gttcgattcc cacccgccgc atagggagaa accgtggaag cagccgaaga
     1741 aaagccaacc tccccccggg aaaaggacga gaagaccgac gcgctcgcca gcggcccgac
     1801 gggagtcgcg gggagcgacc tcgagcgacg ggcagcagcg aggctggtcg aagagcttga
     1861 gagcaagggc cgctcggcgc gggtcc
//
LOCUS       DAOQMF010000405         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_43459_length_1882_cov_2.756431, whole genome shotgun sequence.
ACCESSION   DAOQMF010000405 DAOQMF010000000
VERSION     DAOQMF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43459_length_1882_cov_2.756431"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLV77_07150"
     CDS             <1..857
                     /locus_tag="PLV77_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15659.1"
                     /translation="VWLLGKGRANPLFFAGAATLLTMWALVIVVPVEDRNFDSSRYMY
                     PFLLASVITFGSLIRPGKPGRTLVWVAGIAVAALCLNGVVEQARHNDQQRAGVTVLLR
                     SGLAGVEAVGPTDPSLTADDLATQEEDFFLNLPFRAVDDLEIPSLAAYTAAVDQYGRL
                     GFTGSEIGENGPGSALIADRSAYRVSGIGSPRPLPEGARDLPCRRPAYLLPGPRGVAV
                     PPGLFELRNPSGHPAALRVFRFSHAGTPAGRVPARSAALVGGPQNHGNAGLRMRSDLT
                     LTVCHLAQ"
     gene            complement(851..1723)
                     /locus_tag="PLV77_07155"
     CDS             complement(851..1723)
                     /locus_tag="PLV77_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC15660.1"
                     /translation="MSLLPALIGVAAIILGALGLREITREGGGGSGDGPTAAARPAGQ
                     IADGRGIRSGRSGGGDSGEFPKLGDRIRRAGLEAVLAPRSVLLAKGCCAAVGMLFGSL
                     FTGVLPGRLAPVILIAVAAGGFILPDFMLERTASRRHRGMVAALPDVLDLLAVSVQTG
                     RGLGGCLLDLASSGRGPLIAEMSRAGDDMAWGSGQAAALEGLRQRVGGPEITSFVSTL
                     ERSRRLGSPLADQLRRQSATLRQDQRRTIEEQAARAAPKIQLVIALILVPSVLLLIVA
                     ALAANADSLINVGY"
BASE COUNT          296 a          699 c          615 g          272 t
ORIGIN      
        1 tggtctggct gctcgggaag ggccgggcca atcccctgtt cttcgcgggg gcggcgacgc
       61 ttctgaccat gtgggcgctg gtcatcgtgg ttccggtcga ggatcgcaac ttcgacagct
      121 cccgctacat gtatccgttc ctgctggcaa gcgtgatcac cttcggcagc ctgatcaggc
      181 ccgggaagcc gggccgcacc ctggtctggg tggccggaat cgcggttgcc gcgctctgcc
      241 tcaacggcgt ggtcgaacag gctcgccaca atgaccagca gcgtgccggg gtgaccgtcc
      301 tgcttcgctc cggactggcg ggggtggaag cggtcggccc caccgacccc tcgctgaccg
      361 ccgatgacct cgccacccag gaagaggact tcttcctcaa cctccccttc cgggcggtcg
      421 acgatctgga gatcccctcg ctggccgcct acaccgcagc cgtcgatcag tacggccggc
      481 tgggtttcac cggatcagag atcggcgaga acggccccgg ctccgccctg atcgccgacc
      541 ggtccgctta ccgggtctcc ggcatcggat ctccgcgccc cctgcccgaa ggtgccaggg
      601 acctgccttg ccgacgcccg gcctaccttc tccccggacc acgaggggtc gcggtcccgc
      661 ctggcctttt tgaactccgc aacccttccg gccacccggc cgctctccgc gtcttccgtt
      721 tcagccacgc cggcaccccg gccggccggg taccggcccg ttcggcagct ctggttggcg
      781 gcccgcagaa ccatggcaac gccgggttgc ggatgagaag cgacctgacg ctgaccgtct
      841 gccacctggc tcagtagcca acgttgatca ggctgtcggc gttggcggcg agcgcagcga
      901 cgatcagaag caggaccgac gggaccagaa tcagggcgat caccagctgg atcttcggcg
      961 cggcccgggc ggcctgttcc tcgatcgtcc gtcgctgatc ctgcctgagg gtcgccgact
     1021 gccttcgcag ctggtcggcc agcggcgaac cgagccggcg cgagcgttcc agggtcgaca
     1081 caaacgaggt gatctcggga ccgccgaccc gctgcctgag accttccagt gccgccgcct
     1141 ggccgctacc ccacgccatg tcgtccccgg cccgggacat ctcggcgatc aggggcccgc
     1201 ggccggagct ggccaggtcg agcaggcaac cgccgagacc gcgaccggtc tggaccgaga
     1261 ccgccagcag gtcgagcacg tcgggcaacg cggcgaccat gccccggtgc cggcggctgg
     1321 ccgttcgctc cagcatgaag tcgggcagga tgaaaccgcc ggcggcaacc gcgatcagga
     1381 tgaccggagc cagcctgcct ggcagcacgc cggtgaaaag cgaaccgaaa agcatcccga
     1441 ccgcggcgca gcaacccttg gccagcagca ccgaccgcgg cgccagtacc gcctcgagtc
     1501 ccgcccggcg gatgcggtcg cccagtttgg ggaattcccc cgaatcgcct cccccggacc
     1561 ggccgctccg gatgccacgc ccgtccgcga tctgcccggc cggccgggca gccgcggtcg
     1621 gtccgtcacc ggaacctcct cccccttccc gcgtgatctc ccggagtccg agcgctccga
     1681 ggatgatcgc ggcaaccccg atcagggccg gcagcaggct catgtcacac cgatccgcgc
     1741 gagcctcgag atcaggatga atcccgcgag ctgcaggagg agcgaaagcc cgaccaggac
     1801 cagggccggc accgaaccga ggatcgaggc gaagaagccc ggtctgagca actcggtgaa
     1861 aagtgccgct ccgagcggca tc
//
LOCUS       DAOQMF010000406         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_43479_length_1881_cov_4.530668, whole genome shotgun sequence.
ACCESSION   DAOQMF010000406 DAOQMF010000000
VERSION     DAOQMF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43479_length_1881_cov_4.530668"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLV77_07160"
     CDS             complement(<1..222)
                     /locus_tag="PLV77_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15661.1"
                     /translation="MTAPGKFSIRDAFRGMRLAFEQHRPLLIRVTVLLAALNALSNLF
                     DLTGAAPGFALAVGSFVLLGAVYQGLITAL"
     gene            364..>1881
                     /locus_tag="PLV77_07165"
     CDS             364..>1881
                     /locus_tag="PLV77_07165"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNC15662.1"
                     /translation="METSENKLKFTPFNLAVILAAIFVGLGAGALIYRQHLIDKKDKA
                     EASGKSKPSNKDLNALAPVVGTESGNGDPGSKYDAQFSTTSMWPKFNPDRHYYVTRCV
                     PGRVMVQVRSNPEVKVKVWANPAESGRYAAEARPLPGQDFEVRIMPEGGQAESYEVRC
                     LPSNFPTWRYKRLRKPPKGMFMVSSYLTPGKTGRVWLIVFDQDGTPRWWYSPPYNTLG
                     GQVLYDGTVQFPRGFGDGFGQDARTATDIRDLDGNLVKTVRFDGAPTDGHEYYLLPNG
                     NQLVMSYDPRYGVDLRPIGGRADRGVLDGAFQEQTPDGRVLWSWNSGDHIPLTGTPHR
                     WWIREFNNPHFDRNGKIRYDHFHLNSIEPWGDQYVISTRHTDSIWGISKKTGDIVWKF
                     GGVKTPESLKVVGDDPYGNYPISGNHDARMVGDVLTVHDNGTHVEGRAPRALRYRIDL
                     KNRTATYIGAFTDKAAAPSSHCCGSFRPLGDGWVVSWGDNPWVDGFDSDGQLAFRL"
BASE COUNT          355 a          654 c          581 g          291 t
ORIGIN      
        1 gagagcggtg atcaggccct ggtaaaccgc gcccagcagg acgaagctcc cgaccgcgag
       61 ggcgaatccg ggcgcagcgc cggtcaggtc gaagaggttg gacagcgcgt tcagcgcggc
      121 cagcagaacg gtcacccgga tcagcagcgg acggtgttgc tcgaatgcca gcctcattcc
      181 cctgaaggca tcgcggatcg agaacttgcc gggagcggtc atgggcgccc accctacccg
      241 cagggagtgc ctttatagtt cccggacccc agagagctgc ccaggcgtgg ccgggatgac
      301 acacgcgagg atcgtgtcgc cgtcaccggg ccgaacggga tcgaaccgga aaccctcccc
      361 ggcatggaaa cctccgagaa caaactcaaa ttcacgccgt tcaatctcgc ggtgattctg
      421 gccgcgatct tcgtgggcct gggcgccggt gcgctgatct accgtcagca tctgatcgac
      481 aagaaggaca aggcggaagc ctccggcaag agcaagccga gcaacaagga cctgaacgcc
      541 ctcgctccgg tggtcggcac cgaaagcggc aacggggacc cgggctcgaa gtacgacgcc
      601 cagttcagca caacctcgat gtggccgaag ttcaatcccg accggcacta ctacgtgacc
      661 cgctgcgttc cggggcgggt catggtccag gtccgctcga atccggaagt caaagtcaag
      721 gtctgggcaa atcccgccga gtcgggacgc tacgcggccg aggcccggcc cctgcccggc
      781 caggatttcg aggtcaggat catgcccgag ggcggtcagg ccgagtcata tgaggtccgt
      841 tgcctgccct ccaacttccc gacctggcgt tacaagcgcc tgcgcaagcc gcccaaggga
      901 atgttcatgg tcagctccta cctgaccccc ggcaagaccg gccgggtctg gctgatcgtt
      961 ttcgaccagg acggcacccc ccgttggtgg tacagcccgc cctacaacac cctcggcggc
     1021 caggtgctct acgacggcac ggtccagttc ccccgcgggt tcggtgacgg cttcggccag
     1081 gacgcccgca ccgccaccga cattcgcgac ctcgacggga acctggtcaa gaccgtccgc
     1141 ttcgacggcg ccccgaccga cggccacgag tactacctgc tgccgaacgg caaccagctg
     1201 gtgatgagct acgacccgcg ctacggggtc gacctgcggc cgatcggcgg ccgtgccgac
     1261 cgcggcgtgc tcgatggtgc cttccaggag cagactccgg acggcagggt gctctggtcc
     1321 tggaactccg gtgaccacat tccgctcacc ggcactcccc accgctggtg gatccgcgag
     1381 ttcaacaacc ctcatttcga tcgcaacggc aagatccgct acgaccattt tcacctcaac
     1441 tcgatcgagc cctggggaga tcagtacgtg atctcgaccc gtcacaccga ttcgatctgg
     1501 gggatcagca agaagaccgg cgacatcgtc tggaagttcg gcggcgtcaa gactcccgag
     1561 tcgctgaagg tggtcggtga cgacccctac gggaactacc cgatctccgg caaccacgat
     1621 gcccgcatgg tcggtgacgt cctgaccgtt cacgacaacg gcacccacgt cgagggccgt
     1681 gcccctcgcg ccctccgcta ccggatcgac ctcaagaacc gcaccgcgac ctatatcggc
     1741 gccttcaccg acaaggcggc agcgccgagc agccactgct gcggctcgtt ccgcccgctg
     1801 ggtgacggct gggtcgtctc ctggggtgac aacccctggg tcgacgggtt cgattcagac
     1861 ggccagctgg ccttccgcct c
//
LOCUS       DAOQMF010000407         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_43586_length_1879_cov_2.880482, whole genome shotgun sequence.
ACCESSION   DAOQMF010000407 DAOQMF010000000
VERSION     DAOQMF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43586_length_1879_cov_2.880482"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLV77_07170"
     CDS             complement(<1..259)
                     /locus_tag="PLV77_07170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931869.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNC15663.1"
                     /translation="MSTASILHADLDAFYASVEQRDDPALRGRPVIVGGGVVLAASYE
                     AKAKGIYTTMNGRDARRVCPEAAVVQPRMEAYSKASKAGYEI"
     gene            369..719
                     /locus_tag="PLV77_07175"
     CDS             369..719
                     /locus_tag="PLV77_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC15664.1"
                     /translation="MEYGRTDHSILEQLGTDLQRERLSRNLSQASLAAEAGITRDTVR
                     RLEAGEPVSTLTLVRVLRALGRAESLSGLLPGGGPGPLELFEKGPRGRRRARASSRDD
                     SRRSEWRWGDEDES"
     gene            745..>1879
                     /locus_tag="PLV77_07180"
     CDS             745..>1879
                     /locus_tag="PLV77_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNC15665.1"
                     /translation="MAEVRMWGRRIGAVALADGARAAEFEYTPEFARSGIEVAPVEMP
                     LVEGRRYSFPELSTESFRGLPGMLSDSLPDRFGNALIDAWLARQGREPGSMGAVERLC
                     YTGRRGMGALEYLPATGPEPVESHPVEVEALAELAAEILAERSGIRESFASEDRERAL
                     GEILRVGTSAGGARAKALLAFNPETMEVRSGQLEVPAGFEHWILKFDGVQDQSREIGE
                     PRGYGVIEFAYAQMARAAGIEMTDCHLLEDAGRRHFMTRRFDRTESGRRLHMQSLAAL
                     GHLDFNLPGANSYEQAFRMIDRLGLGRAAVEQQFLRMVFNVVARNQDDHVKNIAFLMD
                     RGGEWALSPAFDLTWAYNPTGRWTSAHQMSVNGKRDGFEPGDFF"
BASE COUNT          309 a          613 c          658 g          299 t
ORIGIN      
        1 agatctcgta gcccgccttg ctggccttcg aataggcctc catccggggc tggacgaccg
       61 ccgcttcggg gcagacccgt cgggcatcgc gaccgttcat cgtcgtgtag atgcccttcg
      121 ccttggcttc gtagctggcg gccaggacga cgccaccacc gacgattacc ggccttcccc
      181 gcagggcggg gtcgtcgcgc tgttcgaccg aggcgtaaaa agcgtcaagg tcggcgtgga
      241 ggatcgaagc cgtgctcacg aacccatgtt cgcaggccag gccgtcggca agactcgacc
      301 cttgttagtg cccgaaatat cagtcataag gtgacatggc tgatatagtg actgatagtt
      361 cagtcactat ggaatacggt cgaacagatc attcaatcct cgagcagctg ggaacggacc
      421 tgcagcgcga gcgcctttcg cgcaacctga gccaggccag cctcgccgcc gaagccggca
      481 tcacgcggga cacggtgcgc cggctcgagg ccggcgagcc ggtttcaacc ctgaccctgg
      541 tccgggttct gcgggccctg gggcgggccg aatcactgag cggcctgctc ccgggtggtg
      601 ggccgggccc gctcgaactg ttcgagaagg gtccgcgggg ccggcgcagg gcccgggcct
      661 cgagccgcga cgattcaaga cggtccgagt ggcgatgggg cgacgaggac gagtcatgaa
      721 cggccgggct gagtaatgac cagcgtggcc gaggttcgaa tgtggggccg gcgcatcggc
      781 gcggtggcgc tcgccgacgg agcgagagcc gccgagttcg agtacacgcc ggagttcgcc
      841 cgcagcggga tcgaggtggc tccggttgaa atgcccctgg tagaaggccg ccgctacagc
      901 ttcccggagc tttcgacgga gagcttccgc gggcttcccg gcatgctttc ggactcgctg
      961 ccggatcgct tcggcaatgc cctgatcgac gcctggctgg cccgccaggg tcgcgagccc
     1021 ggcagcatgg gagcggtcga gcggctctgt tacacgggcc gccggggaat gggggcgctc
     1081 gagtacctgc cggcgaccgg tccagagccg gtcgagagcc atccggtcga ggtcgaggcc
     1141 ctggccgagc tggcggcgga gatccttgcc gagcggtccg ggatcaggga aagcttcgca
     1201 agcgaggacc gggaacgggc gctcggcgag atcctgcggg tcggcacctc ggccggcggg
     1261 gcccgggcca aggccctgct cgccttcaac ccggagacga tggaggtccg ctccggtcag
     1321 ctcgaggtgc cggccgggtt cgagcactgg atcctcaagt tcgacggtgt ccaggaccaa
     1381 agccgcgaga tcggcgagcc gcgcggttac ggagtgatcg agttcgccta cgcacagatg
     1441 gcgagggcgg cagggatcga gatgaccgac tgccacctgc tcgaagatgc tggccggcgc
     1501 cacttcatga cccgccgctt cgaccgcacc gagtccggcc gccgcctgca catgcagtcg
     1561 ctggccgccc tcgggcacct cgacttcaat ctgccggggg caaattccta cgagcaggcc
     1621 ttccgcatga tcgaccgcct cggcctgggc cgggcggcgg tcgagcagca gttcctgcgc
     1681 atggtcttca acgtcgtggc tcgaaatcag gacgaccacg tgaagaacat cgccttcctg
     1741 atggatcgcg gtggcgagtg ggcgctttcc ccggccttcg acctgacctg ggcctacaac
     1801 ccgaccggcc gctggacctc cgcccaccag atgtcggtca acggcaagcg cgacggtttc
     1861 gagcccggag acttcttcg
//
LOCUS       DAOQMF010000408         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_43973_length_1870_cov_4.509091, whole genome shotgun sequence.
ACCESSION   DAOQMF010000408 DAOQMF010000000
VERSION     DAOQMF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43973_length_1870_cov_4.509091"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..440)
                     /locus_tag="PLV77_07185"
     CDS             complement(171..440)
                     /locus_tag="PLV77_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15666.1"
                     /translation="MEGSPFNMFGDPDELRARMQEMADQMQSSQEVAWADNAIKLAVD
                     MTVASIGRLELTGSSDEQAMQVRDAIRVVFPEAVTLVREARQGLR"
     gene            582..1271
                     /locus_tag="PLV77_07190"
     CDS             582..1271
                     /locus_tag="PLV77_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15667.1"
                     /translation="MFESARQRSGGAAGSATLEQVGLILLLAAAFTGLIAATVTGVVE
                     PPGHGLGIRIANRIACGPREPGVCRQHPAVSAYGWPLARALRWLAPDPVARIGPEGTP
                     LAPVDFRYCQRPGCAVPAGDGRLTTANRRLTLFTEVKRLPSGVTGGESAKLEFTYWFY
                     RPSLGWEKVVRQAGPAEIAAASGTRLLLEDSPRLVPLEILPGRNHYDLPAGDEPPWRW
                     KVTPSYEGWSD"
     gene            complement(1279..>1870)
                     /locus_tag="PLV77_07195"
     CDS             complement(1279..>1870)
                     /locus_tag="PLV77_07195"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HNC15668.1"
                     /translation="EVVLDGPVVEIRTPNVRAAIRFGELEPIEVIAPSGKGWAWTRKR
                     AGVPVSGRIITDGKIREVRGFGVDDESAGYHQRNVKWKWSAGIGEATDGRALGWNLVE
                     GINDSPQQSERAIWLEGVPGEPGPVSFDGLDAIRFGEPDEGLVFDFGGAERKRHDNFE
                     LIRSDYTHRFGTFTGRLDGIELASAAGVTEEHAVVW"
BASE COUNT          293 a          649 c          599 g          329 t
ORIGIN      
        1 gtattcatcc ggcggaactg accggcccca cccgcgccgg tcgaccgtga ccgttcgcca
       61 cgaaccggcc agcgagccga ccgcctcgtc gaacaccgag gaatcggtgc cggttccgtg
      121 aatcagcagg accggcggcc cttcgccccg gtcgatgaag ttgagatcgc tcagcggagt
      181 ccctggcgag cttcccggac cagggtcact gcctcgggaa agaccacgcg gatcgcgtca
      241 cgaacctgca tcgcctgctc gtcggagctt cccgtgagct ccagtctgcc gatcgaggcg
      301 acggtcatgt cgacggcgag cttgatcgcg ttgtcggccc aggcgacttc ctgggaggac
      361 tgcatctggt cggccatctc ctgcatccgg gcacgcagtt catcggggtc gccaaacatg
      421 ttgaacggtg aaccttccat gagaccaatc tagcgacccg cagtgtccgg ccgcgcagat
      481 acggccctca cggtcgggat gcgcttcctt tccgctcgcc tgtaactgaa tcgttgcacc
      541 tgtagagcca catgtgagca attcgtccgt agcttcgggt catgttcgaa tcggcaaggc
      601 aaagaagcgg cggggcggct ggatcggcga ccctcgagca ggtcgggctg atcctgcttc
      661 tggctgccgc cttcactggc ctgatcgccg caaccgtgac cggagtcgtc gaaccgccag
      721 ggcacggatt gggaatcagg atcgccaacc ggattgcctg cggcccacgc gagccggggg
      781 tctgtcgcca gcatccggcg gtctcggcct acggctggcc gctcgcccgg gcattgcgct
      841 ggctggcgcc ggaccccgtc gcaaggatcg gccccgaagg gacgccgctg gccccggtcg
      901 acttccgcta ctgccagaga ccgggctgcg cggtgccggc cggcgacggc aggctgacca
      961 ccgccaaccg caggctcacc ctcttcaccg aggtgaagcg gttgccgtcc ggcgtgaccg
     1021 gcggcgaatc ggcgaagctg gagttcacct actggttcta ccggccctcg ctgggctggg
     1081 agaaggtggt ccggcaggcc ggtccggccg agatcgcggc ggcctccgga accaggctgc
     1141 tgctcgagga cagcccgcgt ctggtgcctc tggaaatcct gcccggccga aaccactacg
     1201 acctcccggc cggtgacgaa cccccctggc gctggaaggt gaccccgagc tacgaaggct
     1261 ggtcggactg agaccggctc accagacgac cgcgtgttct tccgtcaccc cggcggccga
     1321 ggcgagttcg atgccgtcga gccttccggt gaatgtgccg aagcggtggg tgtagtcgga
     1381 gcggatcagc tcgaagttgt cgtggcgttt tcgttcggct ccaccgaagt cgaaaacgag
     1441 gccttcatcc ggttctccga accggatcgc atcgagaccg tcgaagctga ccggtcccgg
     1501 ctcaccgggc acgccctcga gccagatcgc ccgttcactt tgctggggag agtcgttgat
     1561 tccttcgacc aggttccagc cgagcgcccg gccgtcagtg gcctcgccga ttcccgccga
     1621 ccacttccat ttgacgttgc gctggtggta cccagctgac tcgtcgtcga ctccgaaccc
     1681 gcgcacttca cggatcttcc cgtcggtgat gattcgcccg ctgaccggca ctccggcccg
     1741 cttgcgggtc catgcccatc ccttcccgct cggggcgatc acttcgatcg gttcgagctc
     1801 accgaagcgg atcgcggccc tcacgttggg ggtccggatc tcgaccacgg gaccgtcgag
     1861 taccacttcc
//
LOCUS       DAOQMF010000409         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44167_length_1865_cov_4.275138, whole genome shotgun sequence.
ACCESSION   DAOQMF010000409 DAOQMF010000000
VERSION     DAOQMF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44167_length_1865_cov_4.275138"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLV77_07200"
     CDS             <1..879
                     /locus_tag="PLV77_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC15669.1"
                     /translation="VKFAGAYHGHSDGLLAEAGSGVATHGIPSGPGIPEAQAAGTVVI
                     PWNDREAVAEALEKHKVAALFAEPVAANMGVVPPEEGYLEFLRQATARAGAMLVFDEV
                     ITGFRVARGGAQELFGIVPDLTVMGKIIGGGLPAAAVGGSRELMERLAPTGDVYQAGT
                     LSGNPLAVSAGLATLRRLDDQAYSRLGVLTDRLVTGLKDLADEHGLTVASAPGLVTLF
                     FSALPVKDFEDAKATDRERHAAFYRAMLERGIWLPPSQFEACFVSLAHDEIMIDRTLM
                     AAAEALAATDPRRAVV"
     gene            876..1454
                     /locus_tag="PLV77_07205"
     CDS             876..1454
                     /locus_tag="PLV77_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15670.1"
                     /translation="MSPSSALDRLADRVREEGSPLTVREPGQASAFGELAAAGPRTSE
                     RAGDYAFVVEAVREGYLCHYGESRILEMPDPDLALLAGDLFYAIGIRGLAELDDLEST
                     GILSDLIRVAAELEAAGRPDLAETLWVAQIVALACGKDDQLSSATAALEAGQTDAARA
                     VREWSIETAAANGLGREFDLAQTAIDSRPSNF"
     gene            1507..>1865
                     /locus_tag="PLV77_07210"
     CDS             1507..>1865
                     /locus_tag="PLV77_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNC15671.1"
                     /translation="MKLPPSEPRGYFEGESMTRRHVFTVASQAIGGVAAAAVVLPVVG
                     FAVAPLFERGEERWEAVGPLSDFNAETYKQVVITETPGIGEAGKTSAYVRTGAEKYNE
                     EPNDVIAVSTRCAHLGCP"
BASE COUNT          291 a          629 c          648 g          297 t
ORIGIN      
        1 gtcaagttcg ccggcgccta ccacggccac tccgacgggt tgctggcgga agctggctcg
       61 ggggtcgcga cccacggcat tccttccggc cccggaattc ccgaagccca ggcagccggc
      121 accgtggtca tcccctggaa tgaccgcgag gcggtggccg aggccctcga gaagcacaag
      181 gtggcggcgc ttttcgccga acccgtggcg gccaacatgg gagtcgtgcc tccggaggag
      241 ggctacctcg aattcctgcg gcaggcgacc gcccgggccg gggcgatgct ggtcttcgat
      301 gaggtgatca ccgggttccg ggtggcccgc ggcggcgccc aggagctgtt cgggatcgtt
      361 cccgacctga ccgtgatggg caagatcatc ggcggtggcc tgcctgccgc cgcggtcggc
      421 ggctcgcgcg agctgatgga gaggctcgcc ccgaccggtg acgtctacca ggcgggcacc
      481 ctttccggaa acccgttggc ggtgtccgcg ggactggcca ccctgcgacg ccttgacgac
      541 caggcttaca gccggctcgg cgtcctgacc gaccggctgg tcacgggcct gaaagacctc
      601 gccgacgagc atggcctcac cgtggcctcc gcgccgggac tggtcacgct cttcttctcg
      661 gccctgccgg tcaaggactt cgaggatgcg aaggcgaccg accgtgaacg gcacgccgcc
      721 ttctaccggg cgatgctgga acgcgggatc tggctgccgc cgtcacagtt cgaggcctgt
      781 ttcgtctcgc tcgcccacga cgagatcatg atcgaccgca cgctgatggc agccgcggaa
      841 gccctggctg ccaccgatcc tcgcagggcc gtcgtttgag tccgtcgagt gccctcgacc
      901 ggctcgccga ccgggtacgc gaggaaggat cacccctgac ggtcagggag cccgggcagg
      961 cttcggcctt cggtgagctc gcggccgcgg gcccccgaac ctcggaacgg gccggcgatt
     1021 acgccttcgt ggtcgaggcg gttcgcgagg gttacctctg ccactacggc gagtcgcgca
     1081 tcctcgagat gcccgacccg gatctcgcgc tcctggccgg agatctcttc tatgcgatcg
     1141 gcattcgcgg gctcgccgaa ctcgacgacc tcgaatccac aggaatcctg tcggatttga
     1201 tccgggtcgc ggccgagctt gaggcggccg gccggccgga cctggcggag accttgtggg
     1261 tagcccagat tgtcgcattg gcatgcggaa aagacgacca gctgagttcc gcgacggcgg
     1321 ccctggaggc gggtcagacg gacgccgcca gggcggtcag ggagtggtca attgaaacag
     1381 cggctgccaa cgggctgggg cgggaattcg acttggcgca gactgcaata gactcccgcc
     1441 cgtcgaattt ctagaagcgt gtccgaaccg ttgaacaaca attccgagtc gggggaagag
     1501 ccccgcgtga aactgccccc gagtgagccc cgtggttact tcgagggcga gtcgatgacc
     1561 cgtcggcatg tcttcaccgt cgccagccag gcgatcggtg gggtggccgc ggcggccgtc
     1621 gtgctgcccg tggtcgggtt cgcggtcgcg ccgctcttcg agcgcggcga ggaacgctgg
     1681 gaagcggttg gcccgctcag tgacttcaac gccgagacct acaagcaggt cgtgatcacc
     1741 gagaccccgg gcatcggcga ggccggcaag acctccgcct acgtccgcac cggcgccgag
     1801 aagtacaacg aggagccgaa cgacgtgatc gccgtctcga cccgttgcgc ccacctcggc
     1861 tgccc
//
LOCUS       DAOQMF010000410         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44172_length_1865_cov_3.841989, whole genome shotgun sequence.
ACCESSION   DAOQMF010000410 DAOQMF010000000
VERSION     DAOQMF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44172_length_1865_cov_3.841989"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PLV77_07215"
     CDS             <1..1247
                     /locus_tag="PLV77_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15672.1"
                     /translation="WYVRAMLYTGGNPVPAVGWGPLHLPQPDQMPLDPRPRFSVAHYI
                     TDPSIYRWWFFPRLDDAFGVLFPLILVMLAAAALWLVFKSRNKIVRVISAAALITAFV
                     YVFTPLTAAGQEFQPRGFFTNTRYLLPGLLLAAVMLPLIRQLREPQARAKKTLIFLTV
                     VYAITVLTSPKWYPSFLPGAIFLTLAIVWAPVGLSWMKDNTRVPKYVIVGCVAAIAII
                     GAIWGRGEEVGYADKHYTRTTLFLQEGGPQDAFEFTRNLKDKRIALAGSGEIFFGQYG
                     FYGVDRSNHVQYIGQEGDNGTYRLITNCREFINTINEGDYDYLITSEYTQDAPEADYW
                     FPVRAWVDGDPNAELVVSEDDITPQPDYVYKINGKLDPDRCAKLDQAAIRQVRTDALQ
                     AIKDREAEAAAAEAAAESSDSQ"
     gene            1296..1369
                     /locus_tag="PLV77_07220"
     tRNA            1296..1369
                     /locus_tag="PLV77_07220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1330..1332,aa:Arg,seq:tct)
BASE COUNT          336 a          614 c          605 g          310 t
ORIGIN      
        1 actggtacgt gcgggcgatg ctctacaccg gcggcaaccc ggtgccggcc gtcggttggg
       61 gcccgctcca cctgccgcag ccggaccaga tgccgctcga cccgcgcccg cggttctcgg
      121 tcgcgcacta catcaccgac ccgagcatct accgctggtg gttctttccc cggctcgatg
      181 acgccttcgg ggtgctgttc ccgctgatcc tggtcatgct cgcagcggcc gcgctctggc
      241 tggtcttcaa gtcccgcaac aagatcgtcc gggtgatctc cgcggccgcc ctgatcaccg
      301 ccttcgtcta tgtgttcacc ccgctgaccg cggccggcca ggagttccag ccgcgcggtt
      361 tcttcaccaa caccaggtac ctgttgccgg ggctccttct ggccgcggtg atgctgccgc
      421 tgatccgcca gttgcgtgag ccgcaggcga gggcgaagaa gaccctgatc ttcctcaccg
      481 tggtctacgc gatcacggtg ctgacctcgc ccaagtggta cccgagcttc ctgcccggcg
      541 ccattttcct caccctggcg atcgtctggg ccccggtcgg cctgagctgg atgaaggaca
      601 acaccagggt gccgaagtac gtgatcgtgg gctgcgtcgc cgcgatcgcg atcatcggcg
      661 cgatctgggg ccgtggtgaa gaggtcggtt acgccgacaa gcactacacc cggaccactc
      721 tcttcctgca ggaaggcggc ccccaggacg ccttcgaatt cacccggaac ctcaaggaca
      781 agcggatcgc gctcgccggt tcgggtgaga tcttcttcgg ccagtacggc ttctacgggg
      841 tcgatcgctc gaaccacgtc cagtacatcg gtcaggaggg tgacaacggc acctaccgtt
      901 tgatcaccaa ctgtcgtgaa ttcatcaaca cgatcaacga aggtgactac gactacctga
      961 tcaccagcga gtacacccag gacgcgccgg aagccgatta ctggttcccg gtccgggcct
     1021 gggttgacgg tgaccccaac gcggagctcg tcgtctcgga agacgacatc actccgcagc
     1081 cggattacgt ctacaagatc aacggcaagc tcgatccaga ccgctgcgcc aagctcgacc
     1141 aggcagcgat ccgccaggtg cggaccgacg cgctccaggc gatcaaggat cgtgaggcag
     1201 aagccgcggc cgcggaagcc gccgcagagt catccgattc gcagtagccg cttccgcccg
     1261 gccagcggcg ggaatggcta gcgttacctt ttcgcgcccc cgtagctcag ctggatagag
     1321 cgccggcctt ctaagccgga tgtcgcaggt tcgaatcctg ccgggggtgc cttcgtcccg
     1381 ctgtgaagaa ggcgatgatc gtgaccacca ggatcaggct gccgtagacg agggcggtgg
     1441 cgtggaggcc gtaccggtcc gaggcgaagc cgccgatcag ggcggggacg ctgaaggcga
     1501 tgtagctgac cacgtaggcg aaggcgaaga gctcgccacg ttcgtcggga tgggggaggg
     1561 tggcaagtac gccgaaggaa ccgagcgccg aggctccgaa gccgattccg gaaatcaggg
     1621 tcccggcaag ggcgagcggg gccaggtcga gcagcaggcc gatcaggttg atcgtgatcc
     1681 cggcggccag ccagcctgcc gcaaagcgga tcacggtcac cggctcgcgg tgacggagcc
     1741 gatagacaac cacggctccg gtcccgcaga gtgccgtgac cacgatcgcg ccggtcaggt
     1801 aggggtcatc ctcgatgaag ctcgatgcga tcgaagggcc cagtgacaga tagagcccgg
     1861 ccagt
//
LOCUS       DAOQMF010000411         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44253_length_1863_cov_3.185288, whole genome shotgun sequence.
ACCESSION   DAOQMF010000411 DAOQMF010000000
VERSION     DAOQMF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44253_length_1863_cov_3.185288"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1863
                     /locus_tag="PLV77_07225"
     CDS             <1..>1863
                     /locus_tag="PLV77_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15673.1"
                     /translation="AMTPDGGYVVAGADRHAGKSLVVRFDADGNLNTDFGDQGELRTA
                     GNGWQQVEVTADGHIVLAGGNNSQMVFERLNADGTPDTGFGGDGGVALDVSSLTLPDG
                     ATRKAAVSAMRILGDGSIRAIGTGFDCTGEWTDSSCPEALLLGVDQDGSLAAGLGTGG
                     VKDLGIRGWLDAASIFADGSALVIRSHYCDPQYGGALTTNTVSASGIVGPDVNLTGGE
                     YSYYCPDLSSPRRAATIDAQGRVLFVTGQSLWRLNPDGTPDAGFGDQGEAPINDSTRF
                     VDGHPYLKTTGITTDDLGRILLSGGLEDGGKGEGGGDTWASMGAVARLTGKGIVDSSF
                     GDGGVRIVWPGRRAYRKLTSQTPVLTSDDRIVIAGLGPAGEDYGFTLAGLENSEATMP
                     VCAGKTADYLGTPGIDKIQAFDAVVFTGGGDDRIIGGSGATICAGEGDDTATLEFGPS
                     RVFGGSGDDTIRLSKGRNDASGGGGDDLIIGGHQQDLIKGGAGEDRLYGGGRDDRLFG
                     GPGQDQIFGQGGRDWLFGGGGQDLLRVGPVRPDPTDYVPAQPGGFMRVTTLGRKASTF
                     VRLPMICEGRDPAPASQLTMRSIDFDPVTGVLSNVGEGSTSLDDDFVWISEIQAR"
BASE COUNT          346 a          557 c          639 g          321 t
ORIGIN      
        1 ggccatgacc cccgacgggg gttatgtggt ggcgggggcc gaccgccacg caggcaaatc
       61 gctggtcgtc aggttcgacg cggacgggaa tctcaacacg gacttcggcg accagggtga
      121 actcaggaca gctggcaacg gctggcaaca ggttgaggta acggccgacg gccacatcgt
      181 cctggccggt ggcaacaact cgcaaatggt cttcgagcgc ctgaatgctg atggcacccc
      241 cgacaccggc ttcggcggag atggcggggt agccctggat gtttcgagcc tgaccctgcc
      301 cgacggggca accaggaagg cagccgtatc ggcaatgagg attctcggcg acgggagcat
      361 cagggcgata ggcacgggct tcgactgcac cggtgaatgg accgattcgt cctgtcccga
      421 ggctttgctg ctcggagtcg accaggacgg atcgcttgcc gcgggcctcg gaacgggcgg
      481 cgtcaaggac ctcggcatca gaggctggct cgacgccgcc tcgatcttcg cggacggcag
      541 cgccttggtc atccgcagcc actactgcga cccccagtac ggcggggccc tgaccaccaa
      601 caccgtctcc gctagcggca tcgtgggacc ggatgtgaac ctgaccggtg gcgagtacag
      661 ctattactgc ccggacttgt cctcaccccg gcgagcggca acgatcgacg cccagggtcg
      721 ggtgctcttc gtcaccgggc aaagcctttg gcgcctgaat cccgatggaa ctccggatgc
      781 cggttttggc gatcaaggtg aggctccgat caacgattcc actcgattcg tggacggcca
      841 cccatatcta aagacaaccg ggataacaac tgacgacctg gggaggattc tcctttccgg
      901 cggcctcgaa gacggcggca agggcgaggg tggtggtgac acctgggcga gcatgggcgc
      961 tgtagcgaga ctgaccggca agggaatcgt cgacagttcc ttcggcgatg gtggtgtccg
     1021 gattgtctgg cccggtcgca gggcgtacag gaaactgacc agccagacac ccgttttgac
     1081 cagtgacgac aggatcgtga tcgcaggcct gggaccggct ggcgaagatt acggtttcac
     1141 tttggccggg ctcgagaaca gcgaggcgac catgccggtt tgcgccggca agacggccga
     1201 ttaccttggg acgccgggca tcgacaagat tcaggccttt gacgctgtgg tcttcaccgg
     1261 cggcggtgat gaccgaatca tcggtggttc cggcgccacg atctgtgccg gagaaggtga
     1321 tgacaccgcg accctggagt ttggtccttc ccgtgtcttc ggtggctcgg gggatgacac
     1381 cattcgactc agcaagggaa gaaacgacgc aagcggcggc ggaggcgacg acctgatcat
     1441 cggcggtcac cagcaggatc tgatcaaggg tggagcagga gaggacaggc tctacggagg
     1501 cggcagggac gacaggctgt tcggcggccc cggacaggat cagatcttcg gccagggtgg
     1561 ccgagactgg ctcttcggcg gtggcggcca ggatctgctc cgggttggcc cggtccgccc
     1621 cgacccgacc gattacgtgc ctgcccagcc cggcggattc atgcgggtca cgactctggg
     1681 acgcaaggcc agcacctttg tccgtcttcc gatgatctgc gagggcagag accccgcgcc
     1741 ggcctcccag ttgacgatga gaagtatcga cttcgacccg gtgaccgggg tgttgtccaa
     1801 cgtgggcgaa ggttctacct cgctggacga cgatttcgtc tggatctcgg agatccaggc
     1861 tcg
//
LOCUS       DAOQMF010000412         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44295_length_1862_cov_3.725512, whole genome shotgun sequence.
ACCESSION   DAOQMF010000412 DAOQMF010000000
VERSION     DAOQMF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44295_length_1862_cov_3.725512"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLV77_07230"
     CDS             <1..574
                     /locus_tag="PLV77_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC15674.1"
                     /translation="YLILEGALIREIDVSGRPSIELLGPGDLIRPWVVPQALDALHVN
                     DEWSLLSTGHIAIVDESFHEKAQGYPSILTVLMDRVVARARWLGFQVALSQLTRIDHR
                     LLLTFRYVAERWGVDDPRGVKIPIRLSHRNLAALIGARRPKVSSALTTLSECDLIEQS
                     PDGTWTFFAADIDPMAVLAGIDDPPEPIGA"
     gene            615..971
                     /locus_tag="PLV77_07235"
     CDS             615..971
                     /locus_tag="PLV77_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC15675.1"
                     /translation="MITGLDFIGIPSTDADRSRAFYVDTLGLRPDEGSDYEFWVGETC
                     FGIWEPDKQGMEFSAEKCSHLAFHVEDIEAARAELEKKGVTFFGDTFDTGVCYMAIFA
                     DPDGNSLMLHHRHTPR"
     gene            1319..>1862
                     /locus_tag="PLV77_07240"
     CDS             1319..>1862
                     /locus_tag="PLV77_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC15676.1"
                     /translation="MDSKVLNRAETTSSIIGLTDEAELWARRDSDPRASEELVNRYLA
                     FAKSVAIRYRSQSESLDDLVQVASLGLVNAVKRFDPDRGVPFIAFASPTITGELKRHF
                     RDRTSAMRLPRSLYDRIGQIDTVVSDLTSQLNREPTVFEIADEMGCPVEEVVEAVEAG
                     HNRQPVSLDQTPGTSEGDQSS"
BASE COUNT          359 a          608 c          549 g          346 t
ORIGIN      
        1 ctacctgatc ctcgagggag ccctgatccg cgagatcgac gtttccggcc ggccttcgat
       61 cgaactgctc ggcccgggcg acctgatccg gccctgggtc gtgccccagg cactcgatgc
      121 cctgcatgtg aatgacgagt ggtcgctgct ttccaccggt cacatcgcga tcgtcgacga
      181 gagctttcac gaaaaggccc agggataccc gagcatcctc accgtgctga tggatcgggt
      241 agtcgcccgc gcgcgctggc tcgggttcca ggttgcgctg agccagctga cacgaatcga
      301 tcaccggctg ctgctcacct tccgctatgt ggccgaacgc tggggtgtcg atgatccgcg
      361 cggggtgaag attccaatcc ggctgagcca ccgaaacctg gccgcactga tcggcgccag
      421 gcggccgaag gtcagctcgg ctctcaccac cctgagcgaa tgcgacctga tcgagcagag
      481 cccggacggt acctggacct tctttgccgc cgacatcgac ccgatggcgg ttctggccgg
      541 aatcgacgac cccccggagc cgatcggcgc ctgaagcagt ttcggccgga ccggtgaccg
      601 gatacctttg gtccatgatc accggactcg atttcatcgg cattccttcc accgacgccg
      661 accgttcgcg cgccttttac gtggacacgc tcggacttcg ccccgacgag ggctccgact
      721 acgaattctg ggtgggcgaa acctgcttcg ggatctggga accggacaag cagggcatgg
      781 agttctcggc cgagaaatgc tcccatctgg cctttcacgt cgaagacatc gaggcggcca
      841 gggccgaact tgaaaagaag ggcgtgacct tcttcggaga caccttcgat accggggtct
      901 gttacatggc gatcttcgcc gatcccgacg gcaattcgct gatgctgcac caccggcaca
      961 cccctcgctg agccgggccc gaaccctgcg ccgggcgctt ctttgaccgg tcgaggcgac
     1021 attttcggtc gaatcgacgc cggaagcgca acgtttgcgt actttgttgt cttttttgtg
     1081 aaacaggctg ttctccactt gttgcgccaa aaacaggggc tcgaaaccca attctgaagc
     1141 cactttctga aggccgttcg gaaacttaag gctttcttta ggtttcggat actggacagt
     1201 tcaccggtcg ctgcttacgg tttccccgaa cgatcaggca ccactgcacg gccccgaaaa
     1261 agagggttcc agggcagcaa ctccggaggg gcactcctcc agaaagagag gctgagcgat
     1321 ggacagcaag gttctaaaca gggcggaaac aacaagcagc atcatcggat tgaccgacga
     1381 ggccgaactc tgggcccgcc gtgacagcga cccccgcgca tcggaggaac tggtcaaccg
     1441 ttacctggcc ttcgccaaga gcgtggcgat caggtaccgc agccagtccg aatccctgga
     1501 cgacctggtc caggtggcca gcctcggcct ggtcaacgcc gtgaagcggt ttgacccgga
     1561 tcgcggagtg ccgttcatcg ccttcgcctc cccgaccatc accggcgagc tcaagcgtca
     1621 ctttcgcgac cggacctcgg cgatgcgcct gccccgcagc ctctatgacc ggatcggcca
     1681 gatcgacact gtcgtttcag acctgaccag ccagctcaac cgcgaaccca cagtcttcga
     1741 gatcgctgac gagatgggat gccccgtcga ggaagtggtc gaagccgttg aggctggcca
     1801 caaccgccaa ccggtttctc tcgaccagac ccccggcacc tcggagggcg atcagagttc
     1861 ac
//
LOCUS       DAOQMF010000413         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44522_length_1857_cov_3.866815, whole genome shotgun sequence.
ACCESSION   DAOQMF010000413 DAOQMF010000000
VERSION     DAOQMF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44522_length_1857_cov_3.866815"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLV77_07245"
     CDS             complement(<1..302)
                     /locus_tag="PLV77_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15677.1"
                     /translation="MEPRKGLAAVFDRPAPTGRQLLLGGGHLAALWALAFVQPLLDLL
                     GNNPDFFVARGNSTGDILILAIGFTLLPPLVLFGIEWVVSKVNARAYYGLHLILMGL"
     gene            complement(334..1740)
                     /locus_tag="PLV77_07250"
     CDS             complement(334..1740)
                     /locus_tag="PLV77_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15678.1"
                     /translation="MAATEPGSFRDRDSRVVVSGDAIYRALSPEGAEDWEAFSASPLL
                     EQLTAAGKVIGTREVDPSVLGESQDLLPTGITKVLEHDRVPFVSYPYEWTFSMLQDAA
                     KLQLELGAAAIDSGLGLKDATPYNVQFIGSQPTFIDIGSFEKITEGEPWIAYRQFCML
                     YLYPLLFQAHKDIPFHPWMRGSIDGIQPIDAIKVFSLRDRLRRGVFLHTSLHARLDRR
                     ANKSGPGSAEENKTKRQVKPGQIKAQLESMNRLVSKLKWKAGETSWSGYRQSNTYSDE
                     DDRRKQAFVGEVAAQLKPGLTWDMGCNDGAYSRIAAESSERVVAFDFDHATVEALYRS
                     LREEGNTKILPLVSNLADPSPALGWRGLERKTLADRGAPDLMLALALIHHVSISANIP
                     IADFLQWARDLETTLLVEFPKRTDPMVRALLANKHEGANPDYEEGNFERELEKRFEVE
                     RREELPSGDRILYLARPR"
BASE COUNT          286 a          582 c          656 g          333 t
ORIGIN      
        1 agccccatca ggatcaggtg caggccgtag taggcccggg cgttgacctt gctgaccacc
       61 cactcgatcc cgaacaggac cagcggcggg agcagggtga agccgatcgc gaggatcagg
      121 atgtcaccgg tcgagttgcc ccgagcgacg aagaagtcgg ggttgttgcc cagcaggtcg
      181 agcagcggct gaacgaaggc gagcgcccag agcgccgcca gatggcctcc gccgagcagc
      241 aactgacgcc cggtcggggc cggccggtcg aagaccgccg ccaatccctt cctcggttcc
      301 acttccgaac tttcgcccgg agcgctcaaa ctcctaccgc ggccgggcca ggtagaggat
      361 ccggtcgccg gaaggcagct cctcgcggcg ctccacttcg aaccgctttt ccagctcgcg
      421 ctcgaagttg ccttcctcgt aatcggggtt ggctccttcg tgcttgttgg ccagaagggc
      481 ccgcaccatc gggtcggtcc gcttcgggaa ctcgaccagg agcgtggtct ccaggtcccg
      541 tgcccactgc aggaagtcgg ctatgggaat gttggcgctg atcgaaacgt ggtggatcag
      601 ggccagggcc agcatcaggt ccggggcgcc gcggtcggcc agggtcttcc gttccagtcc
      661 ccgccagccg agcgccggtg aagggtcggc caggttagag acgaggggca ggatcttcgt
      721 gtttccttct tcccgcagtg accggtagag cgcctcgacg gtggcgtggt cgaagtcgaa
      781 ggcgaccacc cgttcggagc tctcggcggc gatcctcgag taggcgccgt cgttgcagcc
      841 catgtcccag gtcaggccgg gcttcaactg cgccgcgact tcgccgacga aggcctgctt
      901 gcgccggtca tcctcgtccg agtaggtgtt cgactggcgg tagccgctcc atgaggtctc
      961 gccggccttc cacttcagct tcgagaccag gcggttcatc gattcaagct gggccttgat
     1021 ctgccccggc ttgacctgcc tcttggtctt gttctcctcg gcgctgccgg ggccggactt
     1081 gttggcgcgg cggtccagcc tcgcgtggag cgaagtgtgg aggaagacgc cgcggcgcag
     1141 gcggtcacgc agcgagaaga ccttgatcgc gtcgatcggc tggattccgt cgatcgagcc
     1201 gcgcatccag ggatggaagg ggatgtcctt gtgggcctgg aagagcagcg ggtagaggta
     1261 gagcatgcag aactgccggt aggcgatcca cggctcgccc tcggtgatct tctcgaagga
     1321 cccgatgtcg atgaaggtcg gctgcgagcc gatgaactgg acgttgtagg gggtggcgtc
     1381 cttgaggccc aggccggaat cgatcgcggc tgcgccgagc tcgagctgaa gcttcgcggc
     1441 gtcctgcagc atgctgaagg tccactcgta gggataggaa acgaagggga cccggtcatg
     1501 ctcgaggacc ttggtgatcc cggtaggcaa gaggtcctgg ctttcgccga ggaccgaggg
     1561 gtcgacctcc cgggtgccga tcaccttgcc ggcggcggtc agctgttcga gcagggggct
     1621 ggcggagaac gcttcccagt cctcggcgcc ttcggggctc agggcccggt agatcgcgtc
     1681 gcctgaaacg acgacgcggc tgtcccggtc cctgaacgag cccggttctg tggccgccac
     1741 agccctactg ctcggcgggc ttgtcttcgg cgggggcgtc ggtgggggcc gccgacgtgg
     1801 cggtggcggc ggcctccggg gcaggctcgt ccttcttcca gaacatgagc ttgctct
//
LOCUS       DAOQMF010000414         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44629_length_1855_cov_3.072778, whole genome shotgun sequence.
ACCESSION   DAOQMF010000414 DAOQMF010000000
VERSION     DAOQMF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44629_length_1855_cov_3.072778"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1373)
                     /locus_tag="PLV77_07255"
     CDS             complement(147..1373)
                     /locus_tag="PLV77_07255"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNC15679.1"
                     /translation="MKTKALVPIALLLALALLPGAAGAADTYVAPNGSGNACTQASPC
                     SILTGGNEADADSAIILAPGDYGSAVDPIDDRIYTFAPNANYVGRNDVRLFVDASSST
                     ASVTIYSGQKFLGYGTRIISFDEVGVGIYGSARAERIDVRTSIDGSTACRFGNEGMET
                     GGQLTNSLCVADGFGSKGIELAATRNEESAMVMGSTGVSVGNGGAGITASNSGITGGG
                     TGFSRLNVFLSIARAEKGYDLDGGFDSDDNEACISARHTSALSLRPNICGIAEDNPIR
                     ELPGFIDPVSDFHLAAGSPLIDAVKNFSYPIPSVDLEGNPRDATDPDPGAYEFQKPAP
                     PRRCVVPRLRGLKMPQVRNRLRNSNCALGRVTRKKVAAKRKAGRVLRQSPRAGLTLNE
                     GARVRVTVGRKARSRR"
     gene            complement(1510..>1855)
                     /locus_tag="PLV77_07260"
     CDS             complement(1510..>1855)
                     /locus_tag="PLV77_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15680.1"
                     /translation="LLRLRDPASLNALFPDESPAFRDLDAAILEKVVLGGVLGMTETD
                     VEAKRGLAYAKTIGEATAAVDSGEAQAAFILRATPIGQVRAVAEAGETMPPKSTYFFP
                     KIPTGIVFNPLS"
BASE COUNT          306 a          596 c          608 g          345 t
ORIGIN      
        1 tttcatcgcc agcggatcgt ccgagccggc actgacctcg agaatcaggc cggtggtacc
       61 gcggccgatc tccccgacca ccgagtaacc ccctacatgc tcgcctgcct ccggcatcgg
      121 ccggatgcta gacgaccagg ccttgctcac cgccggcttc tcgccttccg gccaaccgtc
      181 acccggaccc tcgctccttc attaagggtc agcccggccc gcggtgactg acgcagcact
      241 cgcccggctt tccgtttcgc cgccactttc ttcctggtga cccgcccgag cgcgcagttg
      301 gagttccgca acctgtttct gacctgcggc atcttcagtc ctcgcagccg cggcaccacg
      361 cagcggcggg gcggggccgg cttctggaat tcgtaggcgc cgggatcggg gtcggtggca
      421 tcccgcgggt ttccttcgag atcaaccgaa gggatcgggt aactgaagtt cttcacggca
      481 tcgatcaggg gtgaacccgc ggcgagatgg aagtcggaga ccggatcgat gaagccgggc
      541 agttcccgga tcgggttgtc ttccgcgatc ccgcagatat tcggccgcag gctgagcgcg
      601 gatgtgtgtc gggccgagat gcaggcttcg ttgtcatcgg agtcgaatcc gccatcgagg
      661 tcgtatccct tctcggcccg ggcgatcgag aggaagacgt tgagccggct gaagccggtt
      721 ccgccgccgg taatgccgct gttcgaagcg gtgattccgg ctccgccgtt gcccaccgaa
      781 actccggtcg aacccatgac catcgctgat tcttcattcc gggtcgcggc cagctcgatg
      841 cctttggacc cgaatccgtc ggcaacgcaa agcgagttgg tgagctggcc tccggtctcc
      901 atcccttcgt tgccgaagcg gcaggcagtg ctgccgtcga tgctcgtcct gacatcgatt
      961 ctttcggcgc gggccgaacc gtagatgccg accccgacct catcgaagct gatgatcctg
     1021 gtcccgtagc cgaggaactt ctggccggag tagatggtca ccgaggcggt gctggacgag
     1081 gcatcgacga acaaccgaac atcgtttcgg ccgacatagt tggcgttcgg cgcgaatgtg
     1141 tagatccggt cgtcgatcgg gtctacggcg gatccgtaat cgccaggagc caggatgatc
     1201 gccgagtccg cgtcggcttc attcccgccg gtgaggatcg agcagggcga cgcctgggtg
     1261 caggcgttgc cggagccgtt cggcgcaaca taggtgtcgg ccgctccggc cgctccgggg
     1321 agcagggcga gggcgaggag cagggcaatc gggacgagcg ctttggtctt catcgagttc
     1381 agcatccggc cgaagccggg acctggctac gacccaaggg ctgaaaccgg ccatcccggc
     1441 cgcttggctt cacctgtgta cggattaacc cggcatacgg ggttaatcga tccacagccg
     1501 ggaatccggt cagctcaggg ggttgaaaac gatgccggtc gggatcttgg ggaagaagta
     1561 ggtcgacttc ggtggcatgg tttctcccgc ctcggcgacc gcccggacct gcccgatcgg
     1621 tgtggcacgg aggatgaagg cggcctgggc ctctcccgaa tcgaccgccg cggttgcctc
     1681 cccgatcgtc tttgcgtagg cgagcccccg tttggcttcg acatcggtct cggtcatccc
     1741 gaggacgccg cccaggacga ccttttcgag gatcgccgca tccagatccc ggaaggctgg
     1801 cgactcatcc gggaacaggg cattgaggga ggcgggatcc cggagccgaa gcagc
//
LOCUS       DAOQMF010000415         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44774_length_1852_cov_3.291597, whole genome shotgun sequence.
ACCESSION   DAOQMF010000415 DAOQMF010000000
VERSION     DAOQMF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44774_length_1852_cov_3.291597"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLV77_07265"
     CDS             <1..513
                     /locus_tag="PLV77_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC15681.1"
                     /translation="GKLIWKRNVGRHNQWGDPARDLPTAAEDLPVTMYPGVAGGVETP
                     MSVSGNSIFVPVSDLCATVVSVTTIVSCPYQTGTGAILALGGGTGRTLWKTPIPTLPD
                     GATTVVGDVVMAPGVDGTLYSLRASDGEILASPKAITGVNAPPAVSGRTVYIGAGAGP
                     GPAVQAWRLP"
     gene            complement(554..1732)
                     /locus_tag="PLV77_07270"
     CDS             complement(554..1732)
                     /locus_tag="PLV77_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC15682.1"
                     /translation="MSPGSSFAPESVIATLRGVLPDVAAASAGAVIAEVPEYSDLRGP
                     EGDTLDHQVELALRGFLVIASESESADSVVPFQPALDAAFALGRGEFRSGRTTDVLLS
                     AYRIGARAAWREWSKVAIGAEIPIEFFARFAEMIFAYIDQLSASSVAGYSHELEASQR
                     SRSRRFEELARRILAGGPAETLQAEAERVGWDAPEAVSAVILANARAQGIRNLVDSRS
                     LIVPGNLPGLEDEDLMLLIVPTVRTSERERIKDQLAGRGAVIGPSREWRRAGQSYARA
                     VKVLNLNRGGHRDDPADTDDFLVELVVGADPEALEDLRREVLSPLEELRPSTTEKLRT
                     TLEAWLLHQGRRDGIAAALHVHPQTVRYRMGQLREIYGDRLDDPAMVLAATVALAAKN
                     "
BASE COUNT          316 a          608 c          623 g          305 t
ORIGIN      
        1 ggaaagctga tctggaagcg aaacgtcggc aggcacaacc agtggggcga cccggccagg
       61 gaccttccga ccgcggcgga ggatctgccg gtcaccatgt atccgggggt cgccggcggc
      121 gtcgaaaccc cgatgtcggt ttccggcaat tccatcttcg tgccggtgtc cgacctctgc
      181 gcgacggtcg tctcggtgac gaccatcgtc tcctgtccct accagaccgg gaccggagcg
      241 atcctggccc tcggtggcgg caccggaagg acgctctgga agaccccgat cccgaccctg
      301 ccggacggag cgacgacggt ggtgggtgat gtcgtcatgg ccccgggagt ggacgggacc
      361 ctttactcgc tgcgggccag tgacggtgaa atcctcgcct ctccgaaggc gatcaccggc
      421 gtcaacgcac cgccggcagt ttccggccga accgtctaca tcggtgccgg agcgggcccc
      481 ggcccggcgg tccaggcctg gcgactgccc tgagcggggc gatcggccgg ccgggcactt
      541 cgaccccggg acttcaattt ttggcggcca gggcaaccgt ggcggcgagg accatggcgg
      601 gatcgtccag ccggtcgccg tagatttccc tcagctgccc catccggtag cgaaccgtct
      661 gcggatgaac gtgaagggcg gcggcgatac cgtcgcggcg accctggtgg agcagccagg
      721 cttcaagcgt ggtccggagc ttctccgtgg tcgagggacg aagttcctca aggggggaga
      781 ggacttccct tctgaggtcc tcaagggctt cggggtcggc cccgaccacc agttcgacca
      841 ggaagtcgtc ggtgtcggcc gggtcatccc ggtgcccgcc gcggttcagg ttgagcacct
      901 tgaccgcccg ggcgtaggac tgaccggccc gccgccactc acgggacggt ccgatcaccg
      961 caccccggcc ggccagctga tccttgatcc gctcgcgctc gctggtccgg accgtcggaa
     1021 cgatcagcag catgaggtcc tcgtcctcga ggccgggcag gttgccggga acgatcaggc
     1081 tgcgcgaatc gaccaggttc cggatcccct gggccctggc gttggcgagg atcacggcgg
     1141 acaccgcttc cggggcatcc cagccgaccc tttccgcctc ggcctgaagg gtctcggccg
     1201 gtccgccagc caggatcctg cgggcaagct cctcgaagcg ccggctccgg gaccgctggc
     1261 tggcttccag ctcgtgggag tagccggcca cgctggaagc agaaagctgg tctatgtagg
     1321 cgaagatcat ctcggcgaat cgggcgaaga actcgatcgg gatttcagcc ccgatcgcca
     1381 cctttgacca ttcccgccag gccgccctgg ccccgatccg gtacgcggag aggagcacgt
     1441 cggtagttcg gccgctccgg aactcgcctc gaccaagggc aaaggcggcg tcaagggcag
     1501 gctggaacgg aaccacggaa tcggctgatt cggattccga ggcgatgacc agaaagcccc
     1561 tcagcgcaag ttcaacctga tggtcgaggg tgtcgccttc gggtcctcgc aggtcgctgt
     1621 attcgggaac ttcagcgatg accgcgccag ccgaggcggc tgcgacatcc ggcaggacgc
     1681 cccgcagggt agcgatcacc gattccggtg cgaaggagga tccagggctc atcgacggca
     1741 atcatagctg caaggattgt tcgcaaacaa taaaaagtga gtccttgatt caaccgggag
     1801 gagcaagata cttctcctcg gatagtccac tcttgggtca tgccttctct gc
//
LOCUS       DAOQMF010000416         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44780_length_1852_cov_3.114079, whole genome shotgun sequence.
ACCESSION   DAOQMF010000416 DAOQMF010000000
VERSION     DAOQMF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44780_length_1852_cov_3.114079"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLV77_07275"
     CDS             <1..207
                     /locus_tag="PLV77_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC15683.1"
                     /translation="RDMGMTIEEAIRAATLGGAAALGRTDIGRLAPGARADAILLEAA
                     SHADFVYRPGVPLVGSTFIGGQQQ"
     gene            complement(264..494)
                     /locus_tag="PLV77_07280"
     CDS             complement(264..494)
                     /locus_tag="PLV77_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNC15684.1"
                     /translation="MAADLAELKGMIEAALPGSSAEVIDETGTGDHLRAEVSAPQFEG
                     QSRINQHRMVKAAVRERFDDGTIHALTIKILD"
     gene            complement(494..>829)
                     /locus_tag="PLV77_07285"
     CDS             complement(494..>829)
                     /locus_tag="PLV77_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNC15685.1"
                     /translation="GHYTAGWIKRGPSGVIGTNKKCATETVAHVFEDVAAGRLLDPAS
                     PDPNSIEELLRERGTHYVSFSGWEKIDAAEVGKGEPHGRPRVKFVRIDEMLETAAEQA
                     EAGAATGGE"
     assembly_gap    830..852
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<853..1822)
                     /locus_tag="PLV77_07290"
     CDS             complement(<853..1822)
                     /locus_tag="PLV77_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNC15686.1"
                     /translation="MDLFDRLPTPYGLVRGGVAPDHPKIKSVTRVYEKTAAKEGFRFF
                     GNVEVGRDLNVEDFEQHYHAIIYTFGAEADRELGIPGEDLPGSYAATAFVGWYNAHPD
                     YADRNFKLDQAKRAVVIGNGNVALDVARMLALDDAELRKTDIADHAIELLDASTIEEI
                     VVLGRRGPAQAAYTNPEVKELGELEQADVIVSPEEIELDPASKAWIESDEADKTARVN
                     VEIVQEYATRQPTGKPKKVALRFLSSPVEIKGGDRVESIVIGRDELVDVDGNLKARDT
                     GEREELACDLILRSVGYTGVPLRGVPFDERRGTILNEDGRVLESHGG"
BASE COUNT          262 a          610 c          610 g          347 t
ORIGIN      
        1 agggacatgg ggatgacgat cgaggaggcg atccgcgcgg cgaccctggg gggcgccgcg
       61 gcgctcggcc gtaccgacat cgggcgactg gcccccggtg cccgcgccga cgccatcctg
      121 ctcgaagccg cttcgcacgc agatttcgtc taccggccgg gagtacccct ggtcgggtcg
      181 accttcattg gcgggcagca gcagtagctt cggaccggtg ccgttggggg caggtccgcc
      241 gcaaccgggg accaatcttg aacctaatcc aggatcttga tggtcagggc gtggatggtg
      301 ccgtcatcga agcgctcccg aaccgcggcc ttgaccatcc ggtgctggtt gatccggctt
      361 tgaccctcga actgcggtgc cgaaacctcg gcccgcaggt gatcgccggt gccggtttcg
      421 tcgatcacct cggcgctcga gccgggcagc gcggcctcga tcatgccctt gagctcggcc
      481 aggtcggcgg ccatcactcg ccccccgttg cagcgcccgc ttcggcctgc tcggccgcgg
      541 tttcgagcat ctcatcgatc cgtacgaact tgacccgggg ccggccatgc ggttcaccct
      601 tgccgacctc ggccgcgtcg atcttctccc agccgctgaa actcacgtag tgggttccgc
      661 gctccctgag gagctcctcg atcgaattcg gatccgggct cgcgggatcg aggagtctgc
      721 cggcggcaac gtcctcgaag acatgggcca cggtttcggt ggcgcatttc ttgttggtgc
      781 cgatcacgcc ggaggggcca cgcttgatcc agccggccgt gtagtggccn nnnnnnnnnn
      841 nnnnnnnnnn nnccccgccg tggctttcga gcacccgtcc gtcctcgttg aggatcgtgc
      901 cccgccgttc gtcgaaggga accccccgca gcgggacccc ggtgtatccg accgagcgca
      961 ggatcaggtc acaagccagt tcctcgcgct cgccggtgtc gcgggctttg agattgccgt
     1021 cgacatcgac cagctcgtcg cggccgatca cgatgctttc gaccctgtcg ccacccttga
     1081 tttcgaccgg cgaggagaga aagcggagcg cgaccttctt cggcttgccg gtgggctggc
     1141 gcgtggcgta ctcctgaacg atctcgacgt tgacccgggc ggtcttgtcg gcctcgtccg
     1201 actcgatcca cgccttgctg gccgggtcga gctcgatctc ctccggactt acgatcacat
     1261 ccgcctgctc gagctcgccg agctccttga cctcggggtt ggtgtaggca gcctgggccg
     1321 ggccgcgccg gccgagcaca acgatttcct cgatcgtcga tgcgtcgagc aactcgatcg
     1381 cgtggtcggc gatgtcggtc ttccgcagtt cggcgtcatc gagggcgagc atgcgggcga
     1441 catcgagggc gacgttgccg ttgccgatca cgaccgcccg tttcgcctgg tccagcttga
     1501 agttccggtc ggcgtaatcg gggtgggcgt tgtaccagcc gacgaaggca gtggcggcat
     1561 agctgccggg cagatcctcg ccggggatgc cgagctcacg gtccgcttcg gccccgaagg
     1621 tgtagatgat cgcgtggtag tgctgctcga aatcctcgac gttcaggtcg cggccgacct
     1681 cgacgttgcc gaagaaacgg aacccctcct tggccgcggt cttctcgtag acccgggtca
     1741 ccgacttgat cttgggatgg tccggggcga caccgccacg caccagcccg tagggagtgg
     1801 gcaggcggtc aaagagatcc acttgggcgt gggtctcggg atccttgatg at
//
LOCUS       DAOQMF010000417         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44887_length_1850_cov_3.060724, whole genome shotgun sequence.
ACCESSION   DAOQMF010000417 DAOQMF010000000
VERSION     DAOQMF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44887_length_1850_cov_3.060724"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLV77_07295"
     CDS             <1..300
                     /locus_tag="PLV77_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15687.1"
                     /translation="RLFGSPRSGRRARVGGSDTFGLGAAGFPARWKGGWGPGTDGKYL
                     VRQMGVMVVNGKQGVVSMAAIPDDGTFESGASMLSAIARSVASRLAGRVSAPTGC"
     gene            350..799
                     /locus_tag="PLV77_07300"
     CDS             350..799
                     /locus_tag="PLV77_07300"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC15688.1"
                     /translation="MDRETVIRELRLEPLPKEGGMYRRKFKDDNSSSIYFLIEPDSPT
                     NLHRLPWPELFHFYAGAPARLHLLGPEPGEARHPVLGIGLEEGERPQMLVPAGTWQAA
                     ETTGAWTLLGTTMAPPFDWDRFELGKRERLLKYWPDQREVIERHLAT"
     gene            complement(809..>1850)
                     /locus_tag="PLV77_07305"
     CDS             complement(809..>1850)
                     /locus_tag="PLV77_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC15689.1"
                     /translation="TAVILYTSGTTGQPKGAELTHSNIRSNIESIGDLFTPTEEDVFF
                     GGLPLFHVFGQTVAMNASIKTGAELTLLPRFDPAKALEIIQRDKVTIFQGVPTMYAAM
                     LQVPNRADYDISSLRLCVSGGAALPVEIIRAFEAEFNTPILEGYGLSETSPVACFGRV
                     DMERKAGTIGTPIRDVELRLVDEEGNVLGVGDVGELQIKGPNVMKGYWQMPEATEKAI
                     DADGWFASGDMATVDEDGYYSIVDRKKEMILRGGYNVYPREVEEVLYEHPAVAEAAVM
                     GVPHDELGEEIVAIIAFKEGESATEDELRDHAKEGVAAYKYPRHILIVDELPKGPTGK
                     ILKREIDLSQVG"
BASE COUNT          308 a          608 c          608 g          326 t
ORIGIN      
        1 cggctattcg gcagcccccg ctccggtcgt cgtgcccggg tcggcggcag cgacaccttc
       61 gggctcggcg cagccggctt ccccgcgagg tggaagggag gctggggccc gggtaccgac
      121 ggcaaatacc tggttcgcca gatgggcgtg atggtcgtga acggcaaaca gggcgtggtc
      181 agcatggccg cgattccgga tgatggaacc ttcgagtccg gcgcgtcgat gctgagcgcg
      241 atcgcgcgat ccgtcgcctc gcgcctggcc ggccgcgttt ccgccccgac tggctgctga
      301 ctgtctgcca gccggggcaa accggagcaa actggcgtat cgtccgggta tggatcgcga
      361 aacggtaatc cgcgagctga ggctcgagcc gctgcccaaa gagggcggca tgtaccggcg
      421 caagttcaag gacgacaact ccagctcgat ctactttctg atcgaaccgg attcaccgac
      481 caaccttcac cggctccctt ggcctgagct ctttcacttt tacgccggcg ccccggcccg
      541 gctgcatctg ctcggtcccg agcccggaga agcgagacac ccggtgctcg gcatcgggct
      601 cgaagagggc gagcgtccgc agatgctcgt gcccgccgga acctggcagg cggccgagac
      661 gaccggcgcc tggaccctgc tcggcacgac catggcgccg cccttcgact gggaccgctt
      721 cgaactaggc aagcgcgagc gcctgctcaa gtactggccc gatcagcgcg aagtgatcga
      781 acgccacctc gccacctgag caccaggctc agccaacctg gctgagatcg atttcgcgct
      841 tcaggatctt gccggtgggg cccttgggga gctcgtcgac gatcaggatg tggcgcgggt
      901 acttgtaggc ggcgacgcct tccttcgcgt ggtcgcgcag ctcgtcttcg gtcgccgact
      961 cgccctcctt gaaggcgatg atcgcgacga tctcctcacc gagttcatcg tgggggacgc
     1021 ccatcacggc ggcctcggcc acggccggat gctcgtagag cacctcttcg acctcgcggg
     1081 gatagacgtt gtaaccgccg cgcaggatca tctccttctt gcggtcgacg atcgagtagt
     1141 aaccgtcctc gtcgacggtc gccatgtcgc ccgaagcgaa ccagccgtcg gcatcgatcg
     1201 ctttctcggt cgcttccggc atctgccagt agcccttcat cacgttcgga cccttgatct
     1261 gaagctcacc gacatcgccg actccgagca cattgccctc ctcgtcgacc agccgcagtt
     1321 ccacatccct gatcggggtg ccgatcgtgc cggcctttcg ctccatgtcg acccggccga
     1381 agcaggcgac cggagaagtc tcggagaggc cgtaaccctc gaggatcggc gtgttgaatt
     1441 cggcctcgaa ggcgcggatg atctcgaccg gaagggcggc accgccggaa acgcagaggc
     1501 ggagcgagct gatgtcgtaa tcggcccggt tcggcacctg gagcatcgcc gcgtacatgg
     1561 tcggcacccc ctgaaagatc gtcaccttgt cacgctggat gatctccaga gccttggccg
     1621 ggtcgaacct cggcagcagg gtgagctcgg ccccggtctt gatcgaggca ttcatggcga
     1681 cggtctgacc gaagacatgg aagagcggca ggccgccgaa gaagacgtcc tcctcggtcg
     1741 gggtgaagag gtcgccgatc gactcgatgt tcgagcggat gttcgagtgc gtgagctcgg
     1801 cgcccttggg ctggccggtg gtgccggagg tgtagagaat gaccgcggtg
//
LOCUS       DAOQMF010000418         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_44926_length_1849_cov_3.341695, whole genome shotgun sequence.
ACCESSION   DAOQMF010000418 DAOQMF010000000
VERSION     DAOQMF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44926_length_1849_cov_3.341695"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLV77_07310"
     CDS             <1..320
                     /locus_tag="PLV77_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15690.1"
                     /translation="NTHGPGENFMSTGNALDVTSSPDGNLWLVSSSSGTLRGGDLLGR
                     YVYNGEPMPSLNLTSVSARMLGGSMTASEAAELLISTVEPGLLARSAPNTPKAVTEGD
                     PPA"
     gene            317..1609
                     /locus_tag="PLV77_07315"
     CDS             317..1609
                     /locus_tag="PLV77_07315"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNC15691.1"
                     /translation="MNKKTRKVAVVGANRIPFARSNGAYSHASNQDMLTSALDGLIDR
                     YGLEGETLGEVAGGAVLKHSRDCDLTRESVLGTRLAAETPAYDVQQACGTGLETTVLV
                     ANKIALGQIDAGIACGADTTSDAPIALNEELRETLLEANRQTTTLGRIKALTNVRPQH
                     LVPAIPRNEEPRTGLSMGEHCAIMAADWEISRSEQDALTVASHHNLAAAYERGFFEDL
                     ITPYLGLRQDQNLRPDSSLVKLAGLKPVFGGPEGTMTAANSTPLSDGASAVLLASEDW
                     AKERGLPILAFVEEAQTAAVDYLHKREGLLMAPAYAMSEMLARANLSLQDFDYYEIHE
                     AFAAQVLCTLKAWEDPDYCRQKLGRSEAMGPIDRSKLNVNGGSLAAAHPFAATGGRIV
                     GSLAKQLHEDGTGRGVISICAAGGQGVVAILEAPGGKK"
     gene            1606..>1849
                     /locus_tag="PLV77_07320"
     CDS             1606..>1849
                     /locus_tag="PLV77_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HNC15692.1"
                     /translation="MSDTYTQIVNAPVVSGLANSLGLPRPVELDRYQPGRPVIDGPVL
                     TGSAPESSLRPAISATLDSIGAERAGTEGPAKALIFD"
BASE COUNT          342 a          661 c          584 g          262 t
ORIGIN      
        1 cgaacacgca cggaccgggg gagaatttca tgtccacggg caacgcgctc gacgtgacca
       61 gcagccccga cggcaacctg tggctggtca gcagcagctc gggcaccctg cgcggtggcg
      121 acctgctagg ccgctacgtc tacaacggcg agccgatgcc cagcctgaac ctgacctcgg
      181 tcagtgcgcg gatgctcggc ggctcgatga ccgcgtccga agcggccgaa cttctcattt
      241 ccacggtcga gccgggcctg cttgcccgga gcgcacccaa cacgccaaag gcagtaaccg
      301 aaggagaccc gcccgcatga acaagaagac ccgcaaggtc gctgtcgtag gcgccaaccg
      361 gatcccgttc gcgagatcca acggggccta ctcgcatgcc tccaaccagg acatgctgac
      421 ttccgccctc gacggcctga tcgatcgcta cggcctcgag ggcgagaccc tcggtgaagt
      481 ggccggcggc gccgttctga agcattcgcg tgactgcgac ctgacccgcg agagcgtgct
      541 cggcacccgg ctggcggccg agacccccgc ctatgacgtt cagcaggcct gcggcaccgg
      601 ccttgaaacg accgtcctgg tcgccaacaa gatcgcgctc gggcagatcg acgccggaat
      661 cgcctgcggg gccgacacca catccgacgc cccgatagcc ctcaacgagg agcttcgcga
      721 gaccctgctg gaagccaacc ggcagacgac gaccctgggc cggatcaagg ccctgaccaa
      781 tgtccggccc cagcacctgg tccccgcgat tccccgcaac gaggaaccgc gcaccggcct
      841 ttcgatgggc gagcactgcg cgatcatggc ggccgactgg gaaatcagcc gaagcgaaca
      901 ggacgcgctg accgtggcca gccaccacaa cctcgcggcc gcctatgaac gcggcttctt
      961 cgaggacctg atcactccct acctcggcct gcgacaggac cagaacctgc ggcccgactc
     1021 ctcgctcgtg aagctggccg ggctgaagcc ggtcttcggc gggccggagg gcacgatgac
     1081 cgcggccaac tcgaccccgc tctcggacgg tgcctcggcc gtgctgctgg cttcagagga
     1141 ctgggccaaa gagcgcggcc tgccaatcct cgccttcgtc gaggaagccc agaccgccgc
     1201 ggtcgattac ctccacaagc gggagggcct cttgatggcc ccggcctacg cgatgtcgga
     1261 gatgctggcc cgggccaacc tctcgcttca ggatttcgac tactacgaga tccacgaagc
     1321 cttcgccgcc caggtgctct gcaccctcaa ggcctgggaa gaccccgact actgtcgcca
     1381 gaaactcggt cgcagcgagg cgatggggcc gatcgaccgg tcgaagctga acgtgaacgg
     1441 cggctcgctg gctgccgccc accctttcgc tgcgaccggc ggccggatcg tcggatcgct
     1501 ggccaagcaa ctgcatgaag acggcaccgg ccgtggcgtg atcagcatct gcgccgccgg
     1561 cgggcagggt gtcgtggcaa ttctcgaagc gcctggaggc aagaagtgag cgacacctac
     1621 acacagatcg tcaacgcacc ggtcgtttcc ggcctggcca actcactggg tttgccgcgg
     1681 ccggtcgagc ttgaccgcta ccagccgggc cgaccggtaa tcgacggacc ggtactgacc
     1741 gggtccgctc cggagagcag cctgcgcccc gcgatcagcg ccaccctcga ctcgattggc
     1801 gccgaacggg cgggaaccga aggtccggcg aaggcactca tcttcgatg
//
LOCUS       DAOQMF010000419         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45062_length_1846_cov_4.286991, whole genome shotgun sequence.
ACCESSION   DAOQMF010000419 DAOQMF010000000
VERSION     DAOQMF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45062_length_1846_cov_4.286991"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLV77_07325"
     CDS             complement(<1..531)
                     /locus_tag="PLV77_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC15693.1"
                     /translation="MPGSGTETVSRRVHDELRRQIVEGELAPGDRIPSERVLAEDFGV
                     NRHAVREALKGLQAAGLIRITHGGATRVLDWRDAGGLEVLLDLGGDPLEPPAEIVRSV
                     VEMRASIGVDAARLCASRASQSERDQVEQLGESAAAMIGTGDEAGLDEAFAAFWLAIV
                     AGSGNIAYRLSLNSLLK"
     gene            556..1056
                     /locus_tag="PLV77_07330"
     CDS             556..1056
                     /locus_tag="PLV77_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC15694.1"
                     /translation="MARLRLLFTGALLAFGLQTLIRRLLLTQFRRSVEALNQGDYGPL
                     LDGFADDAVLHFNDAPHRWAGEHRGRDQIETFLKDFVAAGLQGEILDLWTSGPPWAMT
                     AVARFDDRSTGPDGQELYSNQTMIMVRTRWGKVTEQRDFYVDTTSMIDFDRRLTELGI
                     LPVADG"
     gene            1069..>1846
                     /locus_tag="PLV77_07335"
     CDS             1069..>1846
                     /locus_tag="PLV77_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC15695.1"
                     /translation="MTPGFTPFHHGGSGPPLVLIHGFTDTWRTWDLVIPRLERDFEVL
                     AVTMPGHAGGPAIEGKLTVDTLPSGIEAAMEAAGFTHAHLAGNSLGGWVALQLAARGR
                     ALSVTGLAPAGGWSAEFQNQWALDYFITQQDLVKKAAPYADQIASTPEGRRQATRFIV
                     SDIGLITPEMVVHQLIGAASCPAAMPMVELAREQGFELDAEQIECPVRFVWGTADLLL
                     RWPETAVRFREEWLPAAEFIELDGIGHCPQLEVPAEVAELI"
BASE COUNT          298 a          624 c          609 g          315 t
ORIGIN      
        1 cttgagcagc gagttgagtg aaagccggta ggcgatattg ccggacccgg cgacgatcgc
       61 cagccagaag gcggcgaagg cttcatcgag ccctgcctcg tctccggttc cgatcattgc
      121 ggcggcgctt tcgcccagtt gttcgacctg atcgcgttcc gactggctcg cacggctggc
      181 gcagagcctc gccgcgtcaa ccccgatcga ggctctcatc tcgaccacgg acctgacgat
      241 ctcggccggt ggttcgagcg ggtcgccacc gaggtcgagc agaacctcga gcccgccggc
      301 gtcacgccag tccagcaccc tggtcgcacc gccgtgggtg atcctgatca gcccggctgc
      361 ctggagaccc ttgagcgcct cgcgaacggc atggcgattc acgccgaagt cttcggcgag
      421 gacccgctcg gacgggatgc gatccccggg cgcaagttca ccctcgacga tctggcggcg
      481 cagctcgtcg tggacgcgac gggaaacggt ctctgtgccg gaaccgggca ttgcaggagg
      541 ttttaccatc tccctatggc ccgattgaga ctgctgttca ccggtgcgct cctcgcgttc
      601 ggtcttcaga ccctgattcg caggctgctg ctgacgcaat tccggcggag cgtggaggcc
      661 ctcaaccagg gggactacgg cccgctgctg gacggtttcg ccgatgacgc cgtgcttcat
      721 ttcaatgacg cgccacatcg ctgggccggc gagcatcggg ggcgcgatca gatcgagacc
      781 ttcctcaagg atttcgtagc ggccgggctc cagggagaaa ttctcgacct gtggacttcc
      841 ggcccaccct gggcgatgac cgccgtggcc cgctttgacg accgatcgac cggaccggac
      901 ggtcaggagc tctactcgaa ccagaccatg atcatggtcc ggaccagatg gggcaaggtg
      961 actgagcagc gcgacttcta cgttgacacg accagcatga tcgatttcga ccgcaggctg
     1021 accgagctgg gaatcctgcc ggtcgccgac ggctgagatg cttccggcat gacccccggg
     1081 ttcaccccct tccatcacgg cggatcggga ccgccgctgg tcctgatcca cggtttcacc
     1141 gacacctggc gaacctggga tctggtcatc ccccggcttg aacgcgactt cgaggtgctg
     1201 gcggtgacca tgcccgggca tgccggaggc ccggcgatcg aagggaagct caccgtcgac
     1261 accctcccgt cgggaatcga agccgcgatg gaagcagccg gcttcactca tgcgcacctg
     1321 gccggaaact cgctcggcgg ctgggtcgcc ctgcagctcg cggcgcgcgg tcgcgcgctt
     1381 tcggtcaccg gcctcgcccc ggccgggggc tggtcggcgg agttccagaa ccagtgggcg
     1441 ctcgattact tcatcaccca gcaggacctg gtcaagaagg cggcccccta cgccgatcag
     1501 atcgcgtcga ccccggaagg ccggcgccag gcgacccgct tcatcgtctc cgacatcggc
     1561 ctgatcacgc cggaaatggt ggttcaccag ttgatcggag ccgccagctg cccggccgcg
     1621 atgccgatgg tcgaactggc ccgcgaacag ggcttcgagc tggacgcgga gcagatcgag
     1681 tgcccggtgc ggttcgtctg gggcacggct gacctgctgc ttcgctggcc ggagacagcg
     1741 gtccgctttc gcgaggagtg gctgccggcg gccgagttca tcgaactcga cgggatcggc
     1801 cactgccccc agctggaggt gccggccgag gtcgccgagc tgatcg
//
LOCUS       DAOQMF010000420         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45063_length_1846_cov_4.231156, whole genome shotgun sequence.
ACCESSION   DAOQMF010000420 DAOQMF010000000
VERSION     DAOQMF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45063_length_1846_cov_4.231156"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLV77_07340"
     CDS             complement(<1..774)
                     /locus_tag="PLV77_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNC15696.1"
                     /translation="MNPAAASQLVVKGDLEEIATLIREEQKFLLTTHEGPDGDALGSL
                     LGMHHLLESLGKDSVMFLAAKEFPLPIEYRHLPLEGVFHEPPADIRDRIVVFLDCGNI
                     DRMPVDFLREDGKLMVNIDHHHDNTEFGDLNLVDAGASCTAEIIYELSVLLDVKVSPA
                     MARALYIGIVTDTGKFMYDATRPKTHRVAAELIEAGVDVDDVNRRLYESMPIEKLRLL
                     SRALDNLELHCEGQLIVSYITDEDYRKSGAGEEMTEGIID"
     gene            complement(771..1118)
                     /gene="rbfA"
                     /locus_tag="PLV77_07345"
     CDS             complement(771..1118)
                     /gene="rbfA"
                     /locus_tag="PLV77_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNC15697.1"
                     /translation="MRRVNEVLRQVISDAITNDLSDPRLEMATVTSVDTTPDLRHAKV
                     YFTVLGDDAAKADALKGLGSAHSLLQGKVAAETRMKHTPALAFEYDESIESGMRIDAL
                     LAEGEAKGREDGE"
     gene            complement(1144..>1846)
                     /locus_tag="PLV77_07350"
     CDS             complement(1144..>1846)
                     /locus_tag="PLV77_07350"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNC15698.1"
                     /translation="RLKAETIARRSARKVTLEEIFARAQTGDLKELNIVLKSDVAGSL
                     EALQDEIAKVPQEQVAINIIRAQTGGITESDVMLASASNAVIIGFNVRPLADARRAAQ
                     AEGVDIRTYSVIYKITEDLRAAMEGMLDTVEVEDSIGEAEVREVFKASKVGRIAGCVV
                     TEGRVQRDASVRLVREGTVIWTGQLDSLRRFKDAVQTVEEGQDCGIVLDGYADVKVGD
                     VLEFFSTRQVEQTLS"
BASE COUNT          288 a          590 c          590 g          378 t
ORIGIN      
        1 gtcgatgatc ccttcggtca tctcctcccc ggccccgctc ttgcggtaat cctcgtcggt
       61 gatgtagctg acgatgagct gtccttcgca gtgaagttcg aggttgtcga gggcccggct
      121 cagcaggcgc agtttctcga tcggcatgct ttcgtagagg cgacggttca cgtcatcgac
      181 gtcgactccg gcttcgatca gctcggcggc gacccggtgg gtcttgggcc tggtcgcgtc
      241 gtacatgaac ttgccggtgt cggtgacgat cccgatgtaa agcgcccggg ccatcgcggg
      301 gctgaccttg acgtcgagca ggaccgaaag ttcgtagatg atctcggcgg tgcaggaggc
      361 accggcgtcg accaggttca ggtcaccgaa ttcggtgttg tcgtgatgat ggtcgatgtt
      421 gaccatcagc ttgccgtctt cgcgcaggaa gtcgaccggc atgcggtcga tgttgccgca
      481 atcgagaaag acgacgatcc ggtcgcggat gtcggccggc ggttcgtgga agacgccctc
      541 gagcggcagg tggcggtact cgatcggaag cgggaactcc ttcgcggcga ggaacatcac
      601 ggagtccttg ccgaggcttt ccagcaggtg atgcatcccc agcagcgagc cgagggcatc
      661 tccgtccggt ccctcgtggg tcgtcaagag gaacttctgt tcctccctga tcagggtcgc
      721 gatctcctcg agatccccct tcaccacgag ctggctggcg gcagcgggat tcactcgccg
      781 tcctccctgc ctttggcttc gccttcggcc agcagggcgt cgattcgcat tcccgactcg
      841 atcgattcgt cgtattcgaa ggccagtgcc ggggtgtgct tcatccgggt ctcggccgcc
      901 accttgccct gcaacaggct gtgggcgctg cccaggccct tgagcgcgtc ggcctttgcc
      961 gcatcgtcac ccagcaccgt gaaatagacc ttggcgtgac gcaggtccgg ggtggtgtcg
     1021 accgaggtga cggttgccat ttccagacga ggatcgctca gatcgttggt gatcgcatcg
     1081 gaaatcacct gacgaagcac ctcattcacc ctgcgcatcc gcgccttcga caccgcttgc
     1141 cgcctaactg agggtctgtt cgacctgacg ggtcgagaag aactcgagta cgtcgccgac
     1201 cttcacgtcg gcgtacccgt ccaggacgat gccgcagtcc tggccttcct cgacggtctg
     1261 gaccgcatcc ttgaaacggc gcagcgaatc gagctgcccc gtccagatga cggtcccctc
     1321 cctgacgagc cgcaccgagg cgtcccgttg gaccctccct tcggtgacga cgcatccggc
     1381 gatccggccg accttggagg ccttgaagac ctcccggact tccgcttcac cgatcgaatc
     1441 ctctacctcg accgtgtcga gcatgccttc catggcggcc cgcaggtctt cggtgatctt
     1501 gtagatgacg gagtaggtgc gaatgtcgac cccctcggcc tgcgcggccc gccgggcgtc
     1561 ggcaagcggc ctcacgttga agccgatgat caccgcgttc gaggccgaag cgagcatcac
     1621 gtcggactcg gtgatgccgc cggtctgggc gcggatgatg ttgatcgcga cctgctcctg
     1681 cggcaccttg gcgatttcgt cctgcagcgc ctccagcgaa cccgcgacat cgctcttgag
     1741 cacgatgttg agttccttga ggtcgccggt ctgggcgcgg gcgaagatct cctcgagggt
     1801 gaccttgcgg gcggaacggc gggcgatggt ttcggccttg aggcgg
//
LOCUS       DAOQMF010000421         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45296_length_1841_cov_2.178611, whole genome shotgun sequence.
ACCESSION   DAOQMF010000421 DAOQMF010000000
VERSION     DAOQMF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45296_length_1841_cov_2.178611"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..518)
                     /locus_tag="PLV77_07355"
     CDS             complement(27..518)
                     /locus_tag="PLV77_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15699.1"
                     /translation="MSEDLPVDEVIGALEEYQRRTIDLYREHADDPEACVKGLVRLHL
                     GWTEEDPERAKMVSRYRGEVMAGPGRERLSASNAAYFAQSKEWMETSRNSGAMPSVSF
                     NVLHALVFAPTQELCKHWLGGRLKKQPTEYADAMGDAAWAGILAAGAALQTGQTTAEK
                     RPG"
     gene            complement(575..1492)
                     /locus_tag="PLV77_07360"
     CDS             complement(575..1492)
                     /locus_tag="PLV77_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC15700.1"
                     /translation="MTVRQTIEIHGQPVTYHKMGEGPPVLLVHGITSSSRTWKSVMPR
                     LAETHTVIAPDLLGHGRSAKPQGDYSLGAYASGMRDLLVALDVEKVNVVGHSLGGGVA
                     MQFAYQFPDRISRLVLVDTGGIGRGGNPALRAAALPGAEFVLPLLFTPTLHDAGLKVR
                     NFFAGFGIHGNADVEGVAEGFASLTEADARRAFINTVRSVIDPTGQRVSAADRLYLTA
                     EIPSLIVWGDRDRIIPVAHADLAHELMPGSRLEIFPGAGHFPFNDDPDRFIRIFEEFI
                     ATTEEANLDQDHIRRMLLRRQEEDRAAAA"
     gene            complement(1520..>1841)
                     /locus_tag="PLV77_07365"
     CDS             complement(1520..>1841)
                     /locus_tag="PLV77_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15701.1"
                     /translation="SPGLVIKRAQWKRPTLRLRVAIKPNASAAITVQIRLARDSHARR
                     APRTNRRLITKKLRTGFGFVDLHTAIPTGLKPVLVTVSYPGDKRLLPKTVKRKPGSVS
                     KLTR"
BASE COUNT          288 a          592 c          627 g          334 t
ORIGIN      
        1 ccaggcgtct actgccagcg cgttggtcat cccggcctct tttccgcggt ggtttgaccg
       61 gtctgcaggg cggctccggc ggcgaggatc ccggcccagg ccgcatcacc catcgcatcc
      121 gcgtattcgg tcggctgttt cttcaggcgc cctccgagcc agtgcttgca gagttcctgg
      181 gttggggcga agaccagggc gtgaaggacg ttgaatgaaa cggaaggcat cgcgcccgag
      241 ttgcgggagg tttccatcca ctctttcgat tgcgcgaaat aggcggcgtt cgaagcgctc
      301 agccgctccc tgccggggcc ggccatgacc tccccccggt accgcgagac catcttcgcc
      361 cgctccgggt cttcctcggt ccagcccaga tgaaggcgga ccaggccctt gacgcatgct
      421 tccggatcat cggcgtgctc tcgatacaga tcgatcgtgc gccgctggta ttcctccagg
      481 gcgccgatca cctcgtcgac cggcagatct tcactcatgg gtgtcgagta tgccgaagaa
      541 cggccagatc accgaaggga gtgacgccgc ccggtcaggc cgcggcggcc cggtcttctt
      601 cctgtcggcg cagcagcatg cgccggatgt ggtcctggtc gaggttcgct tcctcggtcg
      661 tggcgatgaa ctcctcgaag atccggatga accggtcggg gtcatcgttg aaggggaagt
      721 ggccggctcc cgggaatatc tcgagccggc tgccgggcat cagctcatgg gcaaggtcgg
      781 cgtgggccac ggggatgatc cggtcccggt caccccagac gatcagcgaa gggatctcgg
      841 cggtcaggta gagccggtcg gcggcggaga cccgctggcc ggtcgggtcg atcaccgaac
      901 gcaccgtgtt gatgaaggcg cgtcgcgcat ctgcctcggt gagcgaagca aacccctcgg
      961 ccacgccctc gacgtcggcg ttcccgtgaa tgccaaaccc ggcgaagaag ttcctcacct
     1021 tgagcccggc atcgtgcagg gtgggcgtga agagcagtgg caggacgaac tcggctccgg
     1081 gcagggcggc ggccctgagg gccggattgc ccccccggcc gatcccgccg gtgtcaacca
     1141 ggaccagccg cgatatgcga tcggggaact ggtaggcgaa ctgcatggcg acgccgccgc
     1201 cgagtgaatg gccgaccacg ttgaccttct ccacatcaag cgcgaccagc aggtcacgca
     1261 tcccgctggc gtaggcgccg agcgagtagt caccctgggg cttggccgag cggccgtgcc
     1321 cgagcaggtc cggcgcgatc accgtgtgcg tctcggccag ccgcggcatc accgatttcc
     1381 aggttcgcga actggaggtg attccgtgga ccagcaggac cggcgggcct tcgcccatct
     1441 tgtggtaggt gaccggctgg ccgtggatct cgatcgtctg gcgtaccgtc atgtccctat
     1501 tttcccgctt ttcggctttc taacgggtga gcttggacac agaaccgggt ttgcgcttca
     1561 ccgtctttgg cagcagccgc ttgtcaccgg ggtaggaaac ggtgaccagg accggtttga
     1621 gcccggtcgg aatcgcggtg tgaaggtcga cgaagccgaa cccggtcctc agctttttgg
     1681 tgatcaatcg ccggttcgtg cggggcgcgc ggcgtgcgtg cgaatcgcgg gccagccgga
     1741 tttgcaccgt gatcgccgcc gaggcattcg gcttgatcgc gacccggagc cgcagggtgg
     1801 gtcgcttcca ctgcgcccgc ttgatcacca gcccggggga g
//
LOCUS       DAOQMF010000422         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45390_length_1839_cov_3.081278, whole genome shotgun sequence.
ACCESSION   DAOQMF010000422 DAOQMF010000000
VERSION     DAOQMF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45390_length_1839_cov_3.081278"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLV77_07370"
     CDS             <1..500
                     /locus_tag="PLV77_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15702.1"
                     /translation="ILPSFVILGAGIGLTMSPMSTAAMNAVPKTKAGIASGVLSMFRM
                     VGGTFGVAALGALFQSEAKTRLASTLGGENGLTASQRSDFAHQLTGGAPHIEGISGAK
                     AERLVAAGHDAFIYGLSNAMTLSVGIAIAGVLVAFFLIGNQAESMDEETREAGEVAEA
                     ASPVA"
     gene            555..1679
                     /locus_tag="PLV77_07375"
     CDS             555..1679
                     /locus_tag="PLV77_07375"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC15703.1"
                     /translation="MTVRSIPRTFARFSLPAALTLICAIAAFMLAGDTARAANGGAVP
                     TGGPARATVKIKLKGVHAHRATVMSRVVIQGTLKPFRNLQTVKVYLYKDGEKFSTRKV
                     KVHKAGGNYGTFRTYLYMRGGNRYGVHAKYWGKAGKRAVGKDTTRHKSWRVRYVGLPH
                     DQCGRVVAGFRKALNRLALVPDQGRCFNGKMERAVLAYRKLNDMTRNARASKTVVRRV
                     FNGKGGYHVRRPELGDHMEAPLSKQVIVFSKGDNPYAIFPIASGKASTPTILGTFSFY
                     WKEPGMNSHGMYYSSYFIRGYATHGYADVPDYPASHGCLRTFIADQPRIYNITEIGMP
                     VYTFGNAFRGKVAGYKVKHSGNGLGPDLGPTGGLDPAAFD"
BASE COUNT          328 a          635 c          575 g          301 t
ORIGIN      
        1 acatcctgcc cagcttcgtg atcctcgggg ccggcatcgg cctgaccatg tcaccgatgt
       61 cgaccgcggc catgaacgcg gtgccgaaga ccaaggccgg catcgcctcc ggcgtgcttt
      121 cgatgttccg gatggtcggc ggaactttcg gcgtggcggc gctcggagcc ctcttccaga
      181 gcgaagcaaa gacccggctg gcgagcaccc tcggcggcga gaacggcctg accgccagtc
      241 agcgttcgga cttcgcccat cagctgaccg gtggcgcgcc ccacatcgaa gggatttccg
      301 gggccaaggc agagcgtctg gtcgccgccg gtcacgatgc cttcatctac ggcctgagca
      361 acgcgatgac cctctcggtc gggatcgcga tcgccggggt gctcgtcgcc ttcttcctga
      421 tcggcaatca ggccgagtcg atggacgagg aaacgagaga ggcaggggaa gttgccgaag
      481 cggcttcacc cgtggcctga tccggccata tctctacaat ttgcggactg gccgcccaac
      541 cgcaggagct tcacttgacc gtccgatcga taccccgtac cttcgcccga ttcagccttc
      601 cggcagcgct caccctgatc tgcgcgattg ccgccttcat gctggctggc gacaccgcca
      661 gagcagccaa tggtggcgcg gttccgaccg gcggtccggc cagggcgacg gtcaagatca
      721 agctcaaggg cgttcatgcc catcgcgcga ccgtgatgag ccgggtcgtg atccagggca
      781 cgctgaagcc cttccgcaac ctccagacgg tcaaggtcta cctctacaag gacggcgaga
      841 agttctcgac ccgcaaggtc aaggtccaca aggccggcgg caattacgga accttccgca
      901 cctaccttta catgcgcggc ggcaaccgct acggcgtgca cgccaagtac tggggcaaag
      961 ccggcaagcg tgcggtcggc aaggacacga ccaggcacaa gtcctggcgg gtccgttacg
     1021 tcggcctgcc ccatgaccag tgtggccgcg tcgtggccgg attccgcaag gccctgaacc
     1081 gcctggcgct ggttcccgac cagggtcgct gcttcaacgg caagatggaa cgcgccgtgc
     1141 tcgcctaccg caagctcaac gacatgaccc gcaacgcgcg ggcctcgaag accgtggtcc
     1201 ggcgtgtctt caacggcaag gggggctacc acgtccgccg gcccgaactg ggcgaccaca
     1261 tggaagcccc gctctcgaag caggtgatcg tcttttcgaa gggtgacaat ccctacgcga
     1321 tcttccccat cgccagcggc aaggcctcga ccccgaccat cctcggcacc ttcagcttct
     1381 actggaaaga gccgggcatg aactcgcacg gcatgtacta ctcctcgtac ttcatccggg
     1441 gctacgcgac ccacgggtac gccgacgttc cggattaccc ggccagtcat ggctgtctga
     1501 ggaccttcat cgccgaccag ccgaggatct acaacatcac cgagatcggg atgccggtct
     1561 acaccttcgg caacgccttc cgtggcaagg ttgccggcta caaggtgaag cattcgggca
     1621 acggactcgg tccggatctc ggcccgaccg gtggcctgga cccggccgcc ttcgactgat
     1681 cccggcccgg ggtagagata tgaagcgcct ggcaacggcc gcagctgcgg tcgggctggt
     1741 cgctgccctg ggggtgctcg ccggcccggt cgctgaacac gccggatcga gcgcgtccgc
     1801 caatggcgtc gcagccggcc cggcggccat gccgatgcc
//
LOCUS       DAOQMF010000423         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45468_length_1837_cov_3.994949, whole genome shotgun sequence.
ACCESSION   DAOQMF010000423 DAOQMF010000000
VERSION     DAOQMF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45468_length_1837_cov_3.994949"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLV77_07380"
     CDS             <1..782
                     /locus_tag="PLV77_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNC15704.1"
                     /translation="CAVSMAVADLVAKREQVPLWQYLDPAATPRTLKCNATITSGEVS
                     DVVTQCETWAADGFEVFKLKAGPGEALELAKAVRSALGHEAKLRIDANGTWGESAGDL
                     LKGLEPFGPELVEEPVTGLAELAALSRGTGIPLVADESVNDPEEAADARFEQACEAAT
                     VKLSKIGGLDASLGGHLPTYLSSALDGPVGIAAAAHVAQTLDPNLPWPGMAHGLATER
                     LFEQTLSATGPLTALNELDPPANGFGLGVELDEGILAACRL"
     gene            complement(796..>1837)
                     /locus_tag="PLV77_07385"
     CDS             complement(796..>1837)
                     /locus_tag="PLV77_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15705.1"
                     /translation="LPPAGRCDIGTTPCQAMFVLREKLGLPVTPVTTTQLAAGELVSG
                     QYTAVVNPATTVAAGAGATALQTFVNNGGGYFSWGTGGATSARNAGMTNVNTTTNGTT
                     DGVPVKVNFNTNSPLSWGFDNGGFLYRVGSGAVTYNPTTLTGNGGSIPNAVEAASYPN
                     PTVRFSYATVAGMTNLFGKTAAVDQAFGAGRVTLLSIDPTFRAWLEGAERLVLNGILY
                     PTGSVITPAGHAAPVTVPAGAIEPGEGEKVTRKELPKVKNRPAKKTHDPANDVRITVG
                     VRQARNLKGAFRAVAPARIKKIARFSKAKNGNVTLIVPNAHSKDGHFAVWVQKLGARL
                     QANGIQPKAIRL"
BASE COUNT          287 a          609 c          634 g          307 t
ORIGIN      
        1 gctgcgcggt gtcgatggcg gtggccgacc tggtcgccaa acgcgagcag gttccgctct
       61 ggcagtacct tgacccggcg gccactccgc gaaccctgaa gtgcaacgcg acgatcacct
      121 ccggcgaagt gagcgacgtt gtcactcagt gcgagacctg ggcggccgac ggattcgagg
      181 tcttcaaact gaaggccggc ccgggtgaag cgctggagct ggcgaaggcc gtccgctccg
      241 ccctgggcca cgaggcgaag ctcagaatcg acgccaacgg cacctggggc gagtcggcag
      301 gggacctgct gaaggggctg gagccgttcg ggcccgaact ggtcgaggaa ccggtcaccg
      361 gactggctga acttgcggcg ttgagcaggg gaaccggaat cccgctggtc gccgacgaat
      421 cggtcaacga tcccgaagaa gccgccgacg cgagattcga gcaggcctgc gaggcggcga
      481 ccgtcaaatt gagcaagatc ggtggcctcg acgcatcact cggcggccac ctgcccacct
      541 atctctcgag cgccctggac ggaccggttg gcatcgccgc cgcggcccat gtcgcccaga
      601 ccctcgatcc gaaccttccc tggccgggca tggcccacgg cctcgcgacc gaacggctct
      661 tcgagcaaac gctttcggcc accggcccgc tgaccgccct taacgaactc gaccccccgg
      721 ccaatggctt tggcctgggg gtcgagctgg acgagggaat actcgctgcc tgccggctct
      781 agggccggcg tcttcctaga ggcgaatcgc cttcggctgg atgccgttgg cctgtagccg
      841 ggcgcccagc ttctgaaccc agacggcgaa gtggccgtcc ttgctgtggg cgttcggcac
      901 gatcagggtg acgttgccgt tcttcgcctt gctgaacctg gcgatcttct tgatcctggc
      961 cggagcgacc gcgcggaagg cacccttcag gttcctggcc tgcctgacgc ccaccgtgat
     1021 ccggacgtca ttggccggat cgtgggtctt cttcgccggg cggttcttga ccttcggcag
     1081 ctccttgcgg gtgaccttct cgccctcgcc gggctcgatc gcaccggccg gcacggtaac
     1141 cggcgccgcg tgaccagccg gcgtgatgac cgatccagtc gggtagagga tgccgttcag
     1201 gaccaggcgc tcggccccct cgagccaggc ccggaaggtc gggtcgatcg agagcagggt
     1261 gacccggccg gcaccgaagg cctgatcaac cgcggcggtc ttgccgaaca agttggtcat
     1321 gccggcgacg gtcgcgtacg agaaccggac agtcgggttc gggtacgagg ccgcctcgac
     1381 ggcgttcggg atcgagccgc cgttgccggt cagcgtggtc gggttgtagg tgaccgcacc
     1441 cgaccccacg cggtagagga atccaccgtt gtcgaagccc catgacagcg ggctgttggt
     1501 gttgaagttc accttcaccg gaacgccgtc ggtggtgccg ttggtcgtcg tgtttacatt
     1561 cgtcataccg gcgttacgag ccgaagtggc accgccggtt ccccagctga agtaaccgcc
     1621 gccgttgttg acgaacgtct ggagcgcggt ggctccggcg cccgccgcaa cggtcgtggc
     1681 cgggttgacg actgccgtgt actggccgct gaccagttcg ccggcagcga gctgggtggt
     1741 cgtgaccggg gtgaccggca gacccagctt ctcgcgcagc acgaacatcg cctggcaggg
     1801 cgtggtcccg atgtcgcagc ggccggcagg cggcagc
//
LOCUS       DAOQMF010000424         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45491_length_1837_cov_3.045455, whole genome shotgun sequence.
ACCESSION   DAOQMF010000424 DAOQMF010000000
VERSION     DAOQMF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45491_length_1837_cov_3.045455"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLV77_07390"
     CDS             complement(<1..573)
                     /locus_tag="PLV77_07390"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC15706.1"
                     /translation="MASSAEPVLVAGSTGYIGRRLALRLVRGGNAVRCMARNPARAGD
                     LEEAGCEVVAGDVLDRESLRWPLSGVRVAYYLVHSMGRGASGDFAESDLKGASNFAEA
                     AAEVGVERIVYLGGLGEGRSRHLRSRHETAQALGSTGIDVTYMRAAVVIGAGSESFQT
                     IYWLVRRLPAMVTPRWTSVRTQPIGIDDVVE"
     gene            complement(557..1570)
                     /gene="glpX"
                     /locus_tag="PLV77_07395"
     CDS             complement(557..1570)
                     /gene="glpX"
                     /locus_tag="PLV77_07395"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNC15707.1"
                     /translation="MGVISHDVRSGSKPDRNLALELVRVTESAALAASRWIGRGDKLA
                     ADGAAVDAMRILLDTVPMDGIVVIGEGEKDEAPMLYNGEKIGDGSPPVVDIAVDPLEG
                     TTLTARGFGGALAVIALSERGTMFDPGPCVYMEKLAAASEFKDLLTFDEPIGEVIRKI
                     GERKGGGPEDVTLTILDRPRHEEVVKEVRESGATIRFITDGDVQAALLAVSDDTGIDL
                     LWGIGGTPEGVLAAAAIKCLGGEIIGKLWPRNDEERQAAIDAGYDLDRVLTADDLVSG
                     DDVFFAATGVTDGDLLDGVRRHKGWASTESLVMRSRSGTVRKIGARHDRAKLREVTGG
                     LFG"
     gene            1598..>1837
                     /locus_tag="PLV77_07400"
     CDS             1598..>1837
                     /locus_tag="PLV77_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC15708.1"
                     /translation="MSARWGLTLPLPGVSLADTKDFVQLAEAEGYTDLWTGETGGPDG
                     FTPLALSAAWTSKVRLGTGIVGVFQRGPALLAQQAA"
BASE COUNT          281 a          651 c          626 g          279 t
ORIGIN      
        1 ctcgaccacg tcgtcgatgc cgatcggctg ggtgcgaacc gaagtccagc ggggcgtgac
       61 catcgccggc aggcggcgaa ccagccagta gatcgtctgg aacgactcgc taccggcacc
      121 gatcaccacg gcggcccgca tgtaggtgac gtcgatcccg gtcgagccca acgcctgcgc
      181 ggtctcgtgg cgggaccgca ggtgccggga gcgaccctcc cccaggccgc ccaggtaaac
      241 gattcgctcg acgccgacct cggccgccgc ttcggcgaag ttgcttgccc ccttcagatc
      301 cgattcggcg aagtcaccgc tcgccccgcg gcccatcgag tgcaccaggt agtaggcgac
      361 ccggactccg gaaagtggcc agcggagcga ttcccggtca agcacgtcgc cggccaccac
      421 ttcacagccg gcttcttcca ggtcccccgc ccgggccggg ttccgggcca tgcagcgaac
      481 ggcgttgccg ccccggacca gcctcagggc cagcctgcgg ccgatgtagc cggtcgaacc
      541 ggcgacaagt accggctcag ccgaagaggc caccagtcac ttcacgaagc ttggcgcggt
      601 cgtggcgggc gccgatcttg cggacggtgc cggaacggga acgcatgacc agagactcgg
      661 tgctcgccca gcccttgtgg cggcggacgc cgtcgagcag gtcaccgtcg gtgacgccgg
      721 tcgcggcaaa gaagacatcg tccccggaga ccaggtcgtc ggccgtcaaa acccggtcga
      781 ggtcgtagcc ggcgtcgatt gccgcctggc gttcctcgtc gtttcggggc cagagcttgc
      841 cgatgatctc cccgccgagg cacttgatcg cagccgcggc gagcacgcct tcgggggtgc
      901 cgccgatgcc ccagagcagg tcgatgccgg tgtcgtccga gaccgccagc agggcggcct
      961 gcacgtcgcc gtcggtgatg aagcggatcg tcgcgccgga ctcccggact tccttgacca
     1021 cttcctcgtg gcgaggccgg tcgagaatgg tcagggtgac gtcttcgggt ccgccgccct
     1081 tgcgctcgcc gatcttgcgg atcacttcgc cgatcggctc gtcgaaggtg agcaggtcct
     1141 tgaactcgga cgcggcggcg agcttctcca tgtagacaca gggcccgggg tcgaacatcg
     1201 tgccgcgctc ggagagggcg atcaccgcga gggcgccgcc gaaaccgcgg gcggtcaggg
     1261 tcgtgccttc gagcggatcg acggcgatgt caaccacggg cgggctgccg tccccgatct
     1321 tctcgccgtt gtagagcatc ggcgcttcgt ccttctcgcc ttcgccgatc accacgatcc
     1381 cgtccatcgg gacggtgtcg agcaggattc gcatcgcgtc gacggcggcg ccgtcggcgg
     1441 caagcttgtc accccgcccg atccaccgcg aggcggcgag ggccgccgac tcggtgacgc
     1501 gaaccagctc cagggccagg ttgcggtcgg gctttgatcc ggatcgaacg tcgtgcgaga
     1561 taacacccat gcggggcagg ctatataggc tgccgccatg agcgcacgct ggggcttgac
     1621 cctccccctg cccggggtct cactggccga caccaaggac ttcgttcagc tggcggaggc
     1681 cgagggttac accgacctct ggaccgggga aaccggcggc ccggacggct tcacgccgct
     1741 cgccctgagt gccgcctgga ccagcaaggt gcggctcggc accggcatcg tcggggtctt
     1801 ccagcgcgga ccggcgctgc tcgcccagca ggccgcc
//
LOCUS       DAOQMF010000425         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45503_length_1837_cov_2.689675, whole genome shotgun sequence.
ACCESSION   DAOQMF010000425 DAOQMF010000000
VERSION     DAOQMF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45503_length_1837_cov_2.689675"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..410
                     /locus_tag="PLV77_07405"
     CDS             45..410
                     /locus_tag="PLV77_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC15709.1"
                     /translation="MDKRRIIGLAGVGTLAGAFSGLFGVGGGTVIVPMLIALYHYGDR
                     LATGTSLAAIIVIAILAAAMHGGIYGNVDLETGLILVGPAIIGVVAGTAIQQRIPEKT
                     VSFLFALLLIGVAIEMVVG"
     gene            410..883
                     /locus_tag="PLV77_07410"
     CDS             410..883
                     /locus_tag="PLV77_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC15710.1"
                     /translation="MDIAIACAICLMGGIVAGLIGVGGGIVFVPAMTLVLSKGQVEAE
                     ATSLMMIALVSVVGAWRQYGYGNVNMRDAVVIGLLSPVGVLVGVVVANSVPERTLRIG
                     FAMLALYMAWRLLRRVFGNDPHDPAEPGTEPLADPGPDPPKGAGPEPLTGSGPAR"
     gene            880..1296
                     /gene="arfB"
                     /locus_tag="PLV77_07415"
     CDS             880..1296
                     /gene="arfB"
                     /locus_tag="PLV77_07415"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNC15711.1"
                     /translation="MKPGIRPRGGPFIPVGEITLRASRSSGPGGQHANVTASRIEAVF
                     EVESSLALNEAEKARIIRKLGPTVTAVSQDARSQTRNRDLALERLETKLAGALSVPRR
                     RRATRPTRASKQKRLESKRHQSRKKQDRRRPGPDYD"
     gene            complement(1298..1645)
                     /locus_tag="PLV77_07420"
     CDS             complement(1298..1645)
                     /locus_tag="PLV77_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15712.1"
                     /translation="MGEPYTDQVLNFNLPTEDFTIPLSIDLPPGQYLLTAKTNVVNQY
                     ASGDNMIECTIWTDEVDGVDRATSDVPYNRIFNLSAMAVADVQGLVELRCVAWNGPAQ
                     LFDTKLIAQPIQG"
     gene            complement(1615..>1837)
                     /locus_tag="PLV77_07425"
     CDS             complement(1615..>1837)
                     /locus_tag="PLV77_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15713.1"
                     /translation="DAAAFGSSRAAGDDHAALAGGDLLGGVEGEADDVQRGSPLRRAS
                     RVSRARAERPEPPERRAPTAWASRTRIRS"
BASE COUNT          286 a          640 c          607 g          304 t
ORIGIN      
        1 ccccggtccg cctgcgggtc gaaaaagtct cctagtatcg cgccatggac aagcggcgca
       61 taatcggcct ggcgggagtg ggcactctgg ccggagcatt cagcggactc ttcggggtcg
      121 gaggcggcac ggtgatcgtg ccgatgctga tcgcgctcta tcactacggc gaccgtctgg
      181 cgaccggaac cagcctcgcc gcgatcatcg taatcgcgat cctggcggcc gcgatgcacg
      241 gcgggatcta cggcaacgtc gatctcgaga ccggcctgat cctggtcggt cccgcgatca
      301 tcggagtggt cgccggcacg gcgatccagc aacgcatccc tgagaagacg gtctcctttc
      361 ttttcgccct gctgctgatc ggagtggcga tcgagatggt ggtcggctga tggacatcgc
      421 gatcgcctgc gccatctgct tgatgggggg aatcgtcgcc ggactgatcg gggtcggcgg
      481 gggcatcgtc ttcgtgccgg cgatgaccct ggtcctttcc aagggccagg ttgaagcgga
      541 agcgacctca ttgatgatga tcgcgctcgt ttcggtggtc ggggcctggc ggcagtacgg
      601 ctacggcaac gtcaacatgc gggacgccgt ggtcatcggc ctgctctcgc cggtcggcgt
      661 gctggtcggg gtggtcgtcg ccaactcggt ccccgagcgg acgctccgga tcggcttcgc
      721 gatgctcgcc ctttacatgg cctggcggct gctcaggcgg gtcttcggca acgacccgca
      781 tgacccggcc gaacctggca ccgagccgct cgccgacccc ggtcccgacc cgccgaaggg
      841 tgccggcccg gaaccgctga ccggctccgg cccggcccga tgaagcccgg catccgaccc
      901 cgcggcgggc ccttcatccc ggtcggcgaa atcaccctgc gagcttcccg gtcctccggc
      961 ccgggtggcc agcacgccaa tgtcaccgcc tcgcggatcg aggccgtctt cgaggtcgag
     1021 tcctcactcg ccctgaacga ggccgagaag gcgaggatca tccggaagct cggaccgacg
     1081 gtcaccgcgg tcagccagga cgcccgctcc cagacccgca accgcgacct cgccctcgag
     1141 agactcgaaa cgaagctggc cggcgcgctt tcggtcccgc gccggcgccg tgcgaccagg
     1201 cccacccgcg cctcgaagca gaagcgcctc gagtcgaagc gccaccagtc ccgcaagaag
     1261 caggaccggc gacgccccgg acccgattac gactgaatca gccctggatc ggctgggcga
     1321 tcagcttggt gtcgaacagc tgggccggac cgttccaggc gacgcagcgc agttcgacca
     1381 gaccctgcac atctgccacg gccatcgccg agaggttgaa gatcctgttg tacggcacgt
     1441 cgctcgtcgc ccggtcgaca ccgtcgacct catcggtcca gatggtgcac tcgatcatgt
     1501 tgtcgccgga tgcgtactgg ttcacaacgt tggtcttggc ggtgagcagg tactggccgg
     1561 gaggcagatc gatcgagagc gggatcgtga aatcctcggt cggcaggttg aagttcagga
     1621 cctgatccgt gtacggctcg cccacgccgt cggcgcccgt cgctccggtg gctccggtcg
     1681 ctccgcccgc gcccggctga cccggctggc ccggcgcagg gggctgcccc gctgcacatc
     1741 gtctgcttct ccttctacgc caccaagcag atcaccaccg gcgagggcgg catgatcgtc
     1801 accagcagcg cggctgctgc cgaacgctgc cgcatca
//
LOCUS       DAOQMF010000426         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45745_length_1831_cov_4.097973, whole genome shotgun sequence.
ACCESSION   DAOQMF010000426 DAOQMF010000000
VERSION     DAOQMF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45745_length_1831_cov_4.097973"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLV77_07430"
     CDS             complement(<1..903)
                     /locus_tag="PLV77_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNC15714.1"
                     /translation="MAQVPPNNVEAEEAVLGSMLVYEPAVNWAVDEVKLKPGDFYLDR
                     HQAIYEAMIDLYAADKACDTLTVTEALVTSGRLDEAGGENYVAELGEKVAAPANARHH
                     AEIVREHAHLRRLLRAAQDIEVWVNDREADAGTLSERAEAELFKVAHRDQEGDFRSLE
                     NMLFEEHQRLEKRISGELKEVGTNTGFPDLDEKTAGFQPSNLIVLAARPSVGKSAFVA
                     NIAENVAVGQDKSVAFFSLEMSEGELSQRFIASHSGIHGDILRKARFKGGDRTWKKIN
                     KSLNALANVPLFVDVTSDLGLLDLR"
     gene            complement(900..1352)
                     /gene="rplI"
                     /locus_tag="PLV77_07435"
     CDS             complement(900..1352)
                     /gene="rplI"
                     /locus_tag="PLV77_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNC15715.1"
                     /translation="MAQAILLADVEGLGEHGTAVEVSPGYLRNYLQPRGLAQPATPGA
                     LEEAIRRKEAAEKAEREAIARAAENASLLSKTVLTINHRAGDDGKLYGSVTTKEIAEA
                     IRDARGIKVDRKKIRLDVPIHQVGTYMIDVEVAGGAIASVKTIVAEEK"
     gene            complement(1352..1609)
                     /gene="rpsR"
                     /locus_tag="PLV77_07440"
     CDS             complement(1352..1609)
                     /gene="rpsR"
                     /locus_tag="PLV77_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNC15716.1"
                     /translation="MAKTREASSRRGRRFSERVRPRKYTKISAENIDYKDLTLLRRFI
                     SDKGKTRSRRVTGLSRKHQAQLSVAVKRAREVALLPYVGEK"
BASE COUNT          286 a          582 c          596 g          367 t
ORIGIN      
        1 ccgcagatcg agcagcccca ggtccgaggt gacgtccacg aacaggggca cgttggccag
       61 ggcgttcagc gacttgttga tcttcttcca ggtccggtcg ccccccttga atctggcttt
      121 ccggaggatg tcgccgtgaa ttccggaatg ggaggcgatg aacctctggg agagttcccc
      181 ttcggacatt tcgagcgaga agaaagccac cgacttgtcc tggcccaccg ccacgttctc
      241 ggcgatgttg gcgacgaagg ctgacttgcc gaccgagggc cgcgccgcga ggacgatcag
      301 gttcgaaggc tgaaagcccg ccgtcttctc gtccaggtcc gggaaccccg tgttggttcc
      361 cacttccttc agctcgccgc tgatccgttt ctcgagccgc tgatgctcct cgaaaagcat
      421 gttctcgagg ctccggaaat cgccctcctg gtccctgtgg gcgaccttga agagttcggc
      481 ttccgcccgt tcggaaagcg tgccggcgtc agcttcgcgg tcgttgaccc agacctcgat
      541 gtcctgggcc gcccgcagca accgtcgcag gtgggcatgc tcgcggacga tctccgcgtg
      601 atggcgggca ttcgccgggg cggcgacctt ctcgcccagt tccgccacgt agttctcgcc
      661 accggcctcg tccagcctgc cgctggtgac cagggcttcg gtcacggtca gggtgtcgca
      721 agccttgtcg gccgcataca ggtcgatcat cgcctcgtag atcgcctgat ggcggtcgag
      781 gtagaaatcg cccggcttca gtttgacttc atctacggcc cagttgacgg caggctcgta
      841 gaccagcatc gagccgagga ctgcctcctc ggcttcgacg ttgttgggtg gaacctgggc
      901 tatttctctt ccgcgacgat cgtcttgacc gaggcgatgg cgccaccggc cacttctacg
      961 tcaatcatgt aggtgccgac ctggtggatc ggcacgtcga gacggatctt cttgcggtcg
     1021 accttgatgc cacgggcatc gcggatcgcc tcggcgatct ccttggtcgt gaccgagccg
     1081 taaagcttgc cgtcatcacc ggcacgatgg ttgatcgtaa gcacggtctt ggaaagcagg
     1141 gaagcgttct cggcggcgcg ggcgatcgct tcgcgctcgg ccttctcggc ggcttccttg
     1201 cggcggatcg cctcctcgag ggcgcccggg gtggcgggct gcgccagacc gcggggctgc
     1261 aggtaattcc ggaggtagcc gggggaaacc tcgacggcgg tgccgtgctc tcccagcccc
     1321 tcgacgtcag cgagcagaat ggcctgggcc attacttctc acccacgtag gggagcaggg
     1381 ccacctcgcg ggcgcgcttt accgcgaccg acagctgggc ctggtgcttg cgggaaaggc
     1441 cggtgacccg gcgggagcgg gtcttgccct tgtccgagat gaaccgacgc agcagggtca
     1501 gatccttgta gtcgatgttc tcggcggaaa tcttcgtgta cttgcggggg cgtacgcgct
     1561 cgctaaagcg gcgcccgcgg cgcgatgacg cttctcgtgt cttagccaat gctttccggt
     1621 gccttacata gttcaagccc gggcaagttc ccgggcattc aggacaagga atcgtaccgg
     1681 ttcccgggcc tgtgcgactt ctgtaccaac cccatcggtc gcaaactccg caaaacatgg
     1741 ttgagcgagg tggttacgcc gcagggtcgg gtgaggatcg ccgggacgga cgcacgcgcg
     1801 gcattcagcc gcgctggtcc tggagtgtcc c
//
LOCUS       DAOQMF010000427         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45794_length_1830_cov_4.219155, whole genome shotgun sequence.
ACCESSION   DAOQMF010000427 DAOQMF010000000
VERSION     DAOQMF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45794_length_1830_cov_4.219155"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLV77_07445"
     CDS             complement(<1..412)
                     /locus_tag="PLV77_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNC15717.1"
                     /translation="MANLPVPSAALIILDGWGIAPPGPGNAISLANTPNFDRLWEQYP
                     TTQLSAQGPDVGLPEGQMGNSEVGHLNLGAGAIVKQDLARIDTAVADGSFFDNEALVA
                     ACQKAVNGNGRLHLIGLVSDGGVHSGWEHLEACVE"
     gene            complement(427..1185)
                     /gene="tpiA"
                     /locus_tag="PLV77_07450"
     CDS             complement(427..1185)
                     /gene="tpiA"
                     /locus_tag="PLV77_07450"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNC15718.1"
                     /translation="MSRRPYVAANWKMNKTASEADEFLDAFLPTRSADGAEVVLCPPW
                     LALQRVAERCAGTPVKVCAQNMFYEDSGAFTGEVSAPMLLDIGVDGTILGHSERRQFF
                     GETDEALARKVPRAIEAGLEPILCVGETQAERESNDTESVLSHQVLTDLAEIHTDDLE
                     KVVIAYEPVWAIGTGLTASPEQAQDACEFIRNLLRSRDPHKAELVRILYGGSVKPANA
                     EELLALPDIDGALVGGAALDPDDFGAIVAAAPAA"
     gene            complement(1206..>1830)
                     /gene="pgk"
                     /locus_tag="PLV77_07455"
     CDS             complement(1206..>1830)
                     /gene="pgk"
                     /locus_tag="PLV77_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNC15719.1"
                     /translation="RPLVVVLGGAKVSDKVGVIDRFLEIADQILIGGAMCFPFYKAQG
                     HAIGGSLCHDDDIPLAREALEKAAAGNCELVLPTDLALGREFSADTDTGELDGVDVPE
                     GWMGLDIGPKSAADYAARVGSAGTVLWNGPMGAFELGPFAGGTRTVAEAVAAAPGYTV
                     VGGGDSVAALVKFGLTDQVNWVSTGGGASLELLEGKDLPGVAALNDK"
BASE COUNT          285 a          623 c          619 g          303 t
ORIGIN      
        1 gttcgacaca ggcctcaagg tgctcccagc cggaatgaac cccgccgtcg gaaaccaggc
       61 cgatcagatg cagccgcccg ttgccgttga ccgccttctg gcaggccgcg acgagggcct
      121 cgttgtcgaa aaaggaaccg tcggcgactg cggtgtcgat ccgggccagg tcctgcttga
      181 cgatcgcacc ggcgccgaga ttgaggtgac cgacctcgga gttgcccatc tgcccttccg
      241 gcaggccgac gtccgggccc tgggcggaga gctgcgtggt cgggtactgc tcccagagcc
      301 ggtcgaagtt gggcgtgttc gccagcgaga tcgcgttgcc cgggccgggc ggggcgatgc
      361 cccagccgtc gaggattatc agcgccgccg atggtacggg caggttcgcc atgggtcccg
      421 ggttccttac gcggccggcg cggcggcgac gatcgcgccg aagtcatccg ggtcgagggc
      481 ggcgccgcca accagggcgc cgtcgatgtc aggcagcgcc aggagctctt ccgcgttggc
      541 cggtttgacc gatccgccgt aaaggatccg caccagctcg gccttgtggg gatcccgcga
      601 gcgcagcagg ttgcgaatga attcgcaggc atcctgggcc tgttccgggg aagcggtgag
      661 gccggtgccg atcgcccaga cgggctcgta ggcgatcacc accttctcca ggtcgtcggt
      721 gtgaatctcc gccaggtcgg tcaggacctg atgcgaaagc accgattcgg tgtcgttcga
      781 ttcccgctcg gcctgggtct caccgacgca gaggatcggc tcgaggccgg cttcgatcgc
      841 cctcggaacc ttgcgggcca gcgcctcgtc ggtctcgccg aagaactggc gccgttcgga
      901 gtggccgagg atcgtgccgt caacgccaat gtcaagcagc atcggggccg agacctcgcc
      961 ggtgaaggct ccggaatcct catagaacat gttctgggcg cataccttga ccggggtgcc
     1021 ggcgcagcgc tcggccaccc gctgaagagc cagccacggc gggcagagca cgacctcggc
     1081 cccgtcggcg gaccgggtcg gcaggaaggc atcgaggaac tcgtccgctt ctgacgcggt
     1141 cttgttcatc ttccagttgg cggctacata cggtcgacgg ctcatgtccg gcctctttcg
     1201 atttgttact tgtcgttcaa tgcggccacg cccgggaggt ccttgccctc cagcagttca
     1261 agcgaagcgc cgcctccggt cgaaacccag ttcacctggt cggtcagccc gaacttgacc
     1321 agggcggcga ccgaatcgcc gccgccgacc acggtgtatc cgggagcggc tgccaccgcc
     1381 tcggccacgg tccgggtgcc gccggcgaat ggtcccagct cgaaggcccc catcggcccg
     1441 ttccagagca cggttccggc cgatccgacc cgggccgcgt aatccgctgc ggacttcgga
     1501 ccgatgtcga ggcccatcca tccttccggc acgtccactc cgtcgagttc cccggtgtcg
     1561 gtgtcggccg agaactcccg gccgagcgcg aggtcggtcg gcaggaccag ctcgcaattc
     1621 ccggcggcgg ccttctccag ggcttcacgg gcgagcggga tgtcgtcgtc atggcagagc
     1681 gagccaccga tcgcgtggcc ctgggccttg tagaagggga agcacatcgc gccgccgatc
     1741 aggatctggt cggcgatctc aaggaagcgg tcgatcactc cgaccttgtc ggaaaccttg
     1801 gccccgccca gcaccacgac cagcggccgg
//
LOCUS       DAOQMF010000428         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45839_length_1829_cov_4.304961, whole genome shotgun sequence.
ACCESSION   DAOQMF010000428 DAOQMF010000000
VERSION     DAOQMF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45839_length_1829_cov_4.304961"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLV77_07460"
     CDS             <1..417
                     /locus_tag="PLV77_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15720.1"
                     /translation="LDDSLAGIPSDVRFECHSGADGVLGVPRLIEGSCNAVFPKLTHI
                     PARNKALVMTRTDSWGTPVLSSAHGVCASNQGTADAFDWRFCWRDWDALRSCAFQGKD
                     CHLALGTGPRHSWLGRWSDGRPVTRLTIHKATPLAP"
     gene            complement(549..1295)
                     /locus_tag="PLV77_07465"
     CDS             complement(549..1295)
                     /locus_tag="PLV77_07465"
                     /inference="COORDINATES: protein motif:HMM:NF018496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HNC15721.1"
                     /translation="MSGPSGTGSHLDIGGVFEKTGDIYSKAFGTFWIVALVLLIPPAI
                     LQWLIGDSALGGLVSAIVNIFATAWLAGGVVRIVQDVEADGQVDYSVGDLLGSVWPRL
                     LAIVGLQIVVGIIIFFGLIFFIIPGIILALVLVVSLPALVVEDGGIFDSMGRSAALTK
                     DNRMRILAVGILMILILVGIAIVAGLLSAITPIIGALAMLVLGVLLYPYIYMLTTVLY
                     FRLVELKEGGVVAVEETVIVEEDAGPPPAV"
     gene            1460..>1829
                     /locus_tag="PLV77_07470"
     CDS             1460..>1829
                     /locus_tag="PLV77_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNC15722.1"
                     /translation="MTSASGTSTLTQDSPRFTGKELRRKASRESHADWEPAPGRDPLK
                     ILLAQGESRVQDLLPIRYGRMSASPFAFYRGGAAIMAADLAHTPATGVRVQACGDAHI
                     SNFGGYAAPDRKLVFDLNDFD"
BASE COUNT          343 a          633 c          565 g          288 t
ORIGIN      
        1 ctcgacgact ccctggcggg gattccctcc gacgtcaggt tcgaatgcca ctcgggcgcc
       61 gatggcgtgc tcggcgtccc tcgcctgatt gagggcagct gcaacgcggt cttcccgaag
      121 ctcacccaca tcccggcccg caacaaggcc ctggtcatga ccagaaccga ctcatgggga
      181 actccggttc tgagttcggc ccacggggtc tgcgcttcca atcagggaac cgccgatgcc
      241 ttcgactggc gcttttgctg gcgtgactgg gatgccctgc gttcctgcgc cttccagggc
      301 aaggactgcc acctcgcgct gggcaccggg ccgcgacaca gctggctcgg aagatggagc
      361 gatggcaggc cggtcacccg gctcaccatc cacaaagcga cgccgctcgc cccctgaacc
      421 cgggcctccg gggctctctc tcaaaagaac aagggccgcg gctggtgctc tgcgcaccgg
      481 ccgcggccct tttgttgact gcgctgatcc gggggctccc acccggaccg gcgcaagttt
      541 gccgccgcct acacggcggg cggcggtccg gcgtcctcct cgacgatcac ggtctcttcg
      601 accgcgacca cgccgccctc cttcagctca accagcctga agtaaagaac cgtcgtcagc
      661 atgtagatgt acgggtagag cagcaccccg agcacgagca ttgcgagcgc cccgatgatc
      721 ggcgtgatcg ccgaaaggag gccggccacg atcgcgatgc cgaccaggat caggatcatc
      781 aggatgccga ccgcgaggat ccgcatgcgg ttgtccttgg tcagtgcggc gctgcggccc
      841 atcgaatcga agattccgcc gtcttcgacc acaagggccg gcagcgagac gaccagcacc
      901 agggcgagaa tgatgcccgg gatgatgaag aagatcagcc cgaagaagat gatgatcccg
      961 accacgatct gaaggccgac gatcgccagc aggcgcggcc agaccgaacc caggagatca
     1021 ccgaccgagt agtcgacctg tccgtccgct tcgacgtcct ggacgatgcg caccaccccg
     1081 ccggccagcc aggcggtggc gaagatgttg acgatcgccg aaacgaggcc gccgagggcg
     1141 ctgtcaccga tcagccactg caggattgcc ggcgggatca ggaggacaag ggcgacgatc
     1201 cagaacgtgc caaaggcctt cgagtagatg tctccggtct tttcgaacac cccgccaatg
     1261 tcgaggtgac tgccggtacc gcttggaccg ctcatttgtt gctcctcctt gggatttcag
     1321 ttcgggaaga cccgtgaaac cgcctttaca cgccatcggc cagatacctc cccgcgggaa
     1381 aagtggttca ttcgggccgg gtggcgattc atccggttgg aatgagaaac agacgaccgg
     1441 cgccaatatc ctcaacgcca tgacgagcgc atcaggcacc tccaccctca cccaggactc
     1501 ccctcgattc accgggaagg aactgcgcag gaaggcttcg agggaaagcc acgcggactg
     1561 ggagccggcg ccgggccgtg accccctgaa gatcctgctg gcccagggcg agagccgggt
     1621 ccaggacctt ctgccgatca ggtacggccg catgtcggcc tcccccttcg ccttttaccg
     1681 gggcggcgcc gcgatcatgg ccgccgacct ggcccacacc ccggccaccg gggtccgggt
     1741 ccaggcctgc ggtgacgccc acatctcgaa cttcgggggc tacgccgcac ccgaccgcaa
     1801 gctcgtcttt gacctgaacg acttcgacg
//
LOCUS       DAOQMF010000429         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45848_length_1829_cov_3.634160, whole genome shotgun sequence.
ACCESSION   DAOQMF010000429 DAOQMF010000000
VERSION     DAOQMF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45848_length_1829_cov_3.634160"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLV77_07475"
     CDS             complement(<1..635)
                     /locus_tag="PLV77_07475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936082.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNC15723.1"
                     /translation="MSESNGNNTGRIEEIQGVVVDVLFPDKLPEIYSAVEIQVKASEG
                     REDQNLICEVQQHLGDDRVRTVAMDATDGLRRGDLVVDTGGPITVPVGDVTLGRIFTL
                     LGEPIDGGDALPADVERWPIHRPSPEATDLTPNQEILETGIKVVDLLAPYVKGGKIGL
                     FGGAGVGKTVLIQELIHNIAQEHGGLSAFCGVGERTREGNDLYLEMTESGV"
     gene            complement(670..1590)
                     /locus_tag="PLV77_07480"
     CDS             complement(670..1590)
                     /locus_tag="PLV77_07480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNC15724.1"
                     /translation="MANRKEVTNQITSVKNIRQITRAMEMVAAARLRRAETRIQHLRP
                     YAQAMRRMTQRAADQAGAVTQVPILEQRETVKRVALLLVTGDRGLAGAFNANILRDGL
                     NRRDELKAEGIETVFYAVGRRGDGALTFRKAEPKQSWTGFTDRPAFSDAREIGETLAS
                     AYVDGEVDRVEVIYNRYVSPLTQHVHRLILLPVQEAEVIGEGTEDEGGEEEGGELAEA
                     HARSSWEYEPEAEQLLATVIPDYVNTSLYRNLLESAASELGSRMTAMRSAAENAETMI
                     DDLTLEMNRVRQAEITQEILEVVGGAEALG"
     gene            complement(1635..>1829)
                     /locus_tag="PLV77_07485"
     CDS             complement(1635..>1829)
                     /locus_tag="PLV77_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15725.1"
                     /translation="PEEDEKELGDAIGNFVDDFGPDFDENGEALAEGESVRVDSAFAE
                     EPDSAGKEEVIENEEAPSAA"
BASE COUNT          256 a          588 c          604 g          381 t
ORIGIN      
        1 atgactccgg actcggtcat ttcgaggtag aggtcgttgc cctcacgggt ccgttcgccg
       61 actccgcaga aggcggagag tccgccgtgc tcctgggcga tgttgtggat cagctcctgg
      121 atcagaacgg tcttgccgac gccggcgccg ccgaacaggc cgatcttgcc gcccttcacg
      181 tacggggcga ggaggtcgac gaccttgatc ccggtttcga ggatctcctg gttcggggtc
      241 aggtcggtcg cttccgggga cggacggtgg atcggccagc gctcgacgtc ggccggcagc
      301 gcgtcgccgc cgtcgatcgg ctcgccaagg agggtgaaga tgcggcccag cgtgacgtcg
      361 ccgaccggaa cggtgatcgg tccgccggtg tcgaccacga ggtcgccgcg acgcaagccg
      421 tcggtggcat ccatggccac ggtgcggacc cggtcatcac cgaggtgctg ctggacttcg
      481 cagatcaggt tctggtcttc gcggccctcg ctggccttga cctggatctc gacggccgag
      541 tagatctcgg gcagtttgtc ggggaagagc acgtcgacca cgacgccctg aatttcctcg
      601 attcgtccgg tgttgttgcc gttgctttct gacatatctc cagttccagt ttcagtcggt
      661 agcgtcgcgt taacccagcg cctcggcgcc gcccacgact tccagaattt cctgtgtgat
      721 ctcggcctga cggacccggt tcatctccag ggtcaggtcg tcgatcattg tttccgcgtt
      781 ctcggccgcg ctgcgcatgg ccgtcatccg ggacccgagt tcggatgctg ccgactcgag
      841 aaggttgcgg taaagcgaag tgttcacgta atcggggatg accgttgcga gcagttgctc
      901 ggcttccggc tcgtattccc aggaggagcg ggcgtgggct tctgcgagct cgccgccctc
      961 ttcttccccg ccttcgtctt cggttccttc accgatgact tcggcctcct ggaccggcag
     1021 caggatcaga cgatgaacgt gctgcgtgag gggcgagacg tagcggttgt agatgacttc
     1081 gacccggtcg acctcgccgt cgacgtaggc cgacgcgagc gtctcgccga tctcgcgggc
     1141 gtccgagaag gccggacggt cggtgaaacc ggtccaggac tgcttcggct ccgccttgcg
     1201 gaaggtgagg gcaccgtcgc cacgacggcc caccgcgtag aagaccgtct cgattccttc
     1261 ggccttcagc tcgtcgcggc ggttgagccc gtcccggagg atgttggcgt tgaaggcacc
     1321 ggccagacca cggtcaccgg tgaccaggag cagggcgacg cgcttgacgg tctcccgctg
     1381 ctcgaggatc ggcacctggg tgaccgcccc ggcctggtcg gccgcacgct gcgtcatgcg
     1441 ccgcatcgcc tgtgcgtagg ggcgcagatg ctggatccgc gtctccgccc ggcgcagccg
     1501 cgcggcagcc accatctcca tcgcgcgcgt gatctggcgg atgttcttga cgctggtgat
     1561 ctggtttgtg acttctttgc ggtttgccat ctagtgcttc cgtattgccg tgaggcggaa
     1621 aggtccgttt ccgcctaggc ggcggaggga gcctcctcgt tctcgatgac ttcttccttg
     1681 ccggccgagt cgggctcttc ggcgaaggcc gaatcaaccc gtaccgactc gccctcggca
     1741 agcgcttcgc cgttctcgtc gaaatcgggt ccgaagtcgt cgacgaagtt gccgatcgcg
     1801 tcgcccagtt ccttttcgtc ttcttccgg
//
LOCUS       DAOQMF010000430         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_45935_length_1827_cov_3.875282, whole genome shotgun sequence.
ACCESSION   DAOQMF010000430 DAOQMF010000000
VERSION     DAOQMF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45935_length_1827_cov_3.875282"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="PLV77_07490"
     CDS             complement(<1..1558)
                     /locus_tag="PLV77_07490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HNC15726.1"
                     /translation="MGKKGIAAGIASLMLLMAAPGAAPAAGPADGAGASARQQSMRQL
                     VVIGLAGRGKPMAAASQIAWPGSSRYRQFLTQAEYRQQFGASPATVRKIKSLRKRHGV
                     RRVILNPTRTAAIVIMNPPAARRLFCASRNLPGKPCRPAGFRSAIRQVSVGEVYPNGP
                     TPRPPKASSSGSGTPHGCNGALKSKALTPNQISTAYEVDALRQRGLDGSGIRVATLSG
                     NVVGTADFRTWAKCFGLPAPKVSQPGMPSGVYDTQSDPDETVLDVEALSFLAPKLDRV
                     LPIFVPQDNKFNNPLLLFLFGALDPARLGGKLPDILSISDGVCEYQFRKAEKWLAQRL
                     LAEAAAIGITTLAASGDAGFLGCDLQKPGAQFPASSRFVTAVGGTNLGLDAGNSILSQ
                     PVWSTYAKKPSQGAGTGGGPSRFWPRPAFQVAPGLTPALQKGRKTRLLPDLAAMASFS
                     PGIATFEKGTGGWGPGGGTSAATPLTAAIVALVLQQEKEAGRPPLGLITPLLYKLSQG
                     GDYSQYFRDVK"
     gene            1623..>1827
                     /locus_tag="PLV77_07495"
     CDS             1623..>1827
                     /locus_tag="PLV77_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15727.1"
                     /translation="MSESSPSPPIRVSIAVALLALSGAWNAGNVGPVASQLAADFDVS
                     LAMVGLVGGTLFFGTGSSFTFDVI"
BASE COUNT          253 a          599 c          653 g          322 t
ORIGIN      
        1 ccttgacgtc gcggaagtac tggctgtaat caccgccctg gctgagtttg tagagcagcg
       61 gggtgatcaa cccgaggggc ggccgtccgg cttctttttc ctgctgcagg acgagcgcga
      121 cgatcgctgc ggtgagtggc gttgcggcgc tggtaccgcc accgggtccc cagccgccgg
      181 tgcccttctc gaaggtggcg atgccggggc tgaaagatgc cattgcggcg agatccggca
      241 aaagccgggt ctttctgccc ttctgcaggg cgggggtgag gccgggcgcg acctggaagg
      301 ccggccgggg ccagaagcgg ctgggtccgc cgcccgttcc ggcaccctgc gaaggcttct
      361 tcgcgtaggt ggaccagacc ggctgggaga ggatcgagtt gccggcatcg agcccgagat
      421 tcgtgccgcc gaccgcggtt acgaaccggg aactggccgg gaactgggcc ccgggcttct
      481 gcaagtcgca gccaaggaag cccgcgtcac ccgaggcggc gagggtggtg atgccgatcg
      541 ccgccgcctc cgcgagcagg cgctgggcca gccacttctc ggccttgcgg aactggtatt
      601 cgcagacccc gtcgctgatc gagaggatgt ccgggagctt gcctcccagc cgggccgggt
      661 caagcgcacc gaaaaggaag agcagcagcg ggttgttgaa cttgttgtcc tgcggtacga
      721 agatcggcag gacgcggtcc agtttcggag ccaggaacga aagcgcctcg acatcgagca
      781 cggtttcgtc cgggtcactc tgggtgtcgt acacgccgct cggcatgccc ggctggctca
      841 ccttcggggc cggcaggccg aagcatttgg cccaggtccg gaaatccgca gtgccgacca
      901 cgttgccgct cagggttgcc acccggatgc ccgagccgtc gaggccccgc tggcgcagtg
      961 cgtcaacctc gtaggcggtc gagatctggt tcggggtgag ggccttcgac ttcagggcgc
     1021 cgttgcagcc gtgcggcgtg cccgagcccg acgaggaggc cttcgggggc cgaggagttg
     1081 gcccgttcgg gtagacctcg ccgaccgaga cctggcggat cgcgctcctg aacccggccg
     1141 gccggcaggg cttcccgggc aggttgcgac tggcgcagaa gagccgcctg gcggcgggcg
     1201 ggttcatgat cacgatcgcc gctgtacggg tcgggttgag gatcacccgg cgcaccccgt
     1261 ggcgcttccg aaggctcttg atcttcctga ccgtcgcggg gctcgccccg aactgctggc
     1321 ggtactcagc ctgggtcagg aactggcggt agcggcttga gcccggccag gcgatctgtg
     1381 acgcggccgc catcggcttg ccccttccgg ccagcccgat caccacgagc tgtcgcatcg
     1441 actgctggcg ggccgatgct ccggcgccgt cggcgggccc cgcggcgggg gcggctcccg
     1501 gcgctgccat cagcagcatc agcgaagcaa tccctgcggc gatccccttc ttccccatgc
     1561 ggggagtatc gccgatggtc cctggccggt ccagcttccg gaactcggtg tagtttctga
     1621 tcgtgtccga gtcctcgcct tcgcctccga tcagggtttc gattgcggtt gcactgctgg
     1681 cgctctccgg agcctggaat gccggcaacg tcgggccggt cgcctcgcag ctggcggcgg
     1741 acttcgacgt ttcgctggcg atggtcggtc tggtcggtgg caccctgttc ttcggaaccg
     1801 gctcctcgtt cactttcgac gtcatct
//
LOCUS       DAOQMF010000431         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46217_length_1820_cov_3.943343, whole genome shotgun sequence.
ACCESSION   DAOQMF010000431 DAOQMF010000000
VERSION     DAOQMF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46217_length_1820_cov_3.943343"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLV77_07500"
     CDS             <1..445
                     /locus_tag="PLV77_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15728.1"
                     /translation="RESLLDEIYSMVLADCRRATVEAIDGAPDDFEASMRAGLTAFAH
                     EWIRDPRRARIQEIEVVGVSPEIERKRRDSIHAFAGIVVDRTLQLGGREQLGALRLDV
                     IALGLIGSVNEQLIDFVLGDLQISLDDLIENQIAAFAAVASQLLE"
     gene            495..1325
                     /locus_tag="PLV77_07505"
     CDS             495..1325
                     /locus_tag="PLV77_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC15729.1"
                     /translation="MSEQQVGPKTIVITGAARGIGLATARILATRGNRVVMSDLDGEL
                     AAAEAEKIGDRAAGFGVDVTDRDAVREHLEMVEREFGPVDVMINNAGIASASPNILEQ
                     DPKITERTIDINLKGAMNGTIEAIRLMEPRRRGQVVNVASLAGIIGVKGLAAYSASKF
                     GLVGFTESIRLEFADKGIKLTCVMPGPVDTGMMDGTSSSPLVSMLAPEEMATGIADAV
                     TTGKPRVSLPRSSWLLARTISVLPPAIGIRVGHWTRIDRIYTEVDPAARAAYEERISR
                     "
     gene            1342..>1820
                     /locus_tag="PLV77_07510"
     CDS             1342..>1820
                     /locus_tag="PLV77_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNC15730.1"
                     /translation="MDRDPGGTRLSGRERTSNGRPGAETVEAIWRIESARLVARLSRL
                     TGEVGLAEDMAQEAFIAALEVWPGSGIPRNPAAWLMTTARNRGIDRIRRDRVLTEKYG
                     QIAHEMELAGPGPGPEQAVLNEDRIGDDVLTLLFTACHPCLTTETQVALTLKAVGGLT
                     "
BASE COUNT          351 a          563 c          617 g          289 t
ORIGIN      
        1 ccgcgagagc ctgctggacg agatctactc gatggtgctg gccgattgcc gacgggcgac
       61 ggtggaagcg atcgacggag ctccggatga cttcgaagcc tcgatgcggg ccgggctcac
      121 cgctttcgcc cacgaatgga tccgtgatcc gcggagggcc cgcatccagg agatcgaggt
      181 ggtcggggtc agccccgaaa tcgagcgcaa gcgccgggac tcgatccatg cgtttgccgg
      241 gatcgtggtc gaccgcacgc ttcagctggg cggcagggaa cagctcggcg cgctgcggct
      301 tgacgtgatc gcgctcggat tgatcggatc cgtgaacgaa cagctgatcg actttgtgct
      361 cggagacctg cagatttccc tcgatgacct gatcgagaac cagatcgccg cctttgccgc
      421 ggtggccagc cagcttctgg aatagccccc ggcgaggtga caatgcttcg tgtcaggtct
      481 gcgagaatga cctcatgagc gaacagcaag tcggaccgaa gacgatcgtg attaccggtg
      541 cggcgagggg catcggcctt gccaccgccc ggattctcgc cacgcggggc aaccgggtcg
      601 tgatgtcgga cctcgacggc gaacttgccg cagcagaagc agagaagatc ggggatcgcg
      661 cggcaggatt cggcgtcgac gtgaccgacc gggacgcggt ccgggaacac ctcgagatgg
      721 tcgagaggga gttcggtccg gttgacgtga tgatcaacaa cgccggcatc gcttccgcct
      781 cgccgaacat cctcgaacag gatccgaaga tcaccgagcg cacgattgac atcaacctga
      841 agggtgcgat gaacgggacc atcgaggcga ttcgcctgat ggaacccagg cgacggggcc
      901 aggtcgtgaa cgtggcttcg ctggccggga ttatcggagt caaaggcctc gctgcctact
      961 ccgcatccaa gttcggtctg gtcggattca cggaatcgat ccggctggaa ttcgccgaca
     1021 aggggatcaa actcacctgc gtgatgcccg gtccggtaga caccgggatg atggacggca
     1081 cctcttcctc gccgctggtt tcgatgctcg cacccgaaga gatggcaacc ggaatcgccg
     1141 acgcagtgac caccggcaag ccgcgggtct cgctgccacg atcgagctgg ctgctggccc
     1201 gcacgatttc ggtactgcca ccggcgatcg ggatccgggt cgggcactgg acgcggatcg
     1261 accggatcta caccgaggtt gacccggccg ccagggccgc atacgaagaa cgaatcagcc
     1321 gctgatcaat atcgtgggtc catggaccgg gacccgggtg gcacaaggct ttcaggtcgc
     1381 gaaaggacct cgaacggccg cccgggcgcg gaaacggttg aggcgatctg gcggatcgag
     1441 tccgcccggc tcgtagccag gctctcgcgg ctgaccgggg aggtcggcct ggccgaggac
     1501 atggcccagg aagcgttcat cgcggccctc gaagtctggc cagggtcggg cattccccgc
     1561 aaccccgcag cctggctgat gaccaccgca agaaaccggg ggatcgaccg gatccgcagg
     1621 gacagggtcc tgaccgaaaa gtacgggcag atcgcccatg agatggagct tgccggaccc
     1681 gggcccggcc cggagcaggc ggtgttgaac gaggaccgga tcggcgatga cgtcctgacc
     1741 ctgctcttca cggcctgcca tccatgcctg accaccgaaa cacaggtcgc gctgactctg
     1801 aaggcggtcg gcggcctcac
//
LOCUS       DAOQMF010000432         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46337_length_1818_cov_3.311968, whole genome shotgun sequence.
ACCESSION   DAOQMF010000432 DAOQMF010000000
VERSION     DAOQMF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46337_length_1818_cov_3.311968"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..781)
                     /gene="sepF"
                     /locus_tag="PLV77_07515"
     CDS             complement(218..781)
                     /gene="sepF"
                     /locus_tag="PLV77_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNC15731.1"
                     /translation="MALRDSWHRTLVYFGLAEEGDHHEYDDYEDDFVEEDEYESRRGR
                     RSRDANVRRLPTSRRGRHDEIDDIFADDDPMPAATPRSRTLRSVGGDNGGDDVQVHLV
                     IPRNFNDAQQVADQFKRKVPVILNLQTTDHELSKRLIDFASGLTYALDGGMQRIAEKV
                     FLLTPRNVEVSAEEKARLIDKGFFNQS"
     gene            complement(983..1723)
                     /locus_tag="PLV77_07520"
     CDS             complement(983..1723)
                     /locus_tag="PLV77_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNC15732.1"
                     /translation="MKWKEEAGLPWLEADLTGDATVCFSSRLGGVSQGPFESLNLGIL
                     TEDRREDVLANRRKLAVAAGFDADRVSMGRQVHGAELNWAGPGIPGAYSAPGPDPPPE
                     ADGQLTKETLRPVLVLVADCLPVALLGERGLGMLHCGWRGLASGIIDRAASEIGVRAA
                     AIGPGIGPCCFEVGPEVEEAFAGLGPGLMNGRNFDLPEVARRLLARAGVEEVESAGVC
                     TYCDERFFSHRRDRGKTGRQAGIAWLNG"
BASE COUNT          306 a          650 c          549 g          313 t
ORIGIN      
        1 ctcgaccgcc cggccgccaa cctccttggc cagcgccgtg gcgcgcccgg aaccggagtc
       61 cgtgaagagc atctccgacg gggaatcgtc ttcggcgttc gcccagccgc gcgccattgc
      121 cgcggccatg tttccggaac ctatgaatcc gacaaccatg ctgcgaagaa tatggatatg
      181 ccccggtgat ccgcccaccg gggatcccgc gaaccggtca ggactggttg aagaagccct
      241 tgtcgatcaa ccgggccttc tcttccgccg aaacctcaac gttgcgcggg gtcagcagga
      301 agaccttctc ggcgatccgc tgcatgccgc cgtcaagcgc gtaggtgagc ccggatgcga
      361 agtcgatcag gcgcttggaa agctcgtggt cggtggtctg cagattgagg atgaccggca
      421 ccttgcgctt gaactggtcc gccacctgct gcgcgtcgtt gaagttccgc gggatgacca
      481 ggtgaacctg gacgtcatcg ccgccgttgt cgccacccac cgagcgcagc gtccggctgc
      541 gcggcgtggc tgccggcatc ggatcgtcgt cggcaaagat gtcgtcgatc tcgtcgtggc
      601 gtccgcgcct cgaggttggc aggcgacgaa cgttcgcatc acgggaacga cggccacgac
      661 ggctttcgta ttcgtcctct tcgacgaagt cgtcctcgta atcgtcgtat tcgtgatggt
      721 cgccttcttc ggcgagaccg aagtaaacga gtgtgcggtg ccaggaatcg cgtagtgcca
      781 taggtatgcg gtgttttcgg tccggggagg ctgattcctc ccggttgggg gtccggtcgc
      841 agcaattatc actgaattac ccgttttgca gcgaaaatca cgctcgaagg caacggccac
      901 ccatcccgtc cgacccgtgg cccagaccgc gaaagcccac tttgcgccac ataccggcac
      961 aaaatgggca ttcgccgccc gcctaaccgt tgagccaggc gattccggcc tgccggccgg
     1021 tctttccccg atccctccga tgcgagaaga agcgttcgtc gcaatacgtg cagacgccgg
     1081 ccgactcgac ctcttccacc ccggcccggg ccagcagccg ccgcgcgact tccggcagat
     1141 cgaaattccg cccgttcatc agccccgggc caagccccgc gaaggcttcc tcgacctcgg
     1201 gaccaacctc gaaacagcaa ggaccgatcc ccgggccgat tgcggctgcc cgcaccccga
     1261 tctcacttgc cgcccgatcg atgatcccgg aagccaggcc ccgccagccg cagtggagca
     1321 tcccgaggcc cctttcaccg agcagggcga ccggcaggca gtcggcgacc agcaccagaa
     1381 cgggtctcag ggtttctttc gtcagctggc cgtcagcctc cggcggcgga tcgggcccgg
     1441 gggccgaata ggcaccggga atcccggggc cggcccagtt cagctccgcg ccgtgcacct
     1501 gccggcccat tgagacccgg tcggcatcga aaccggccgc gaccgcgagc tttcgtcgat
     1561 tcgcgagcac gtcctcccgc cggtcctcgg tgaggatgcc gagattgagc gactcgaacg
     1621 gtccctggct gaccccgccc agcctgctgc tgaaacagac cgttgcgtcc ccggtcaggt
     1681 cagcctcaag ccagggcaga ccggcttcct ctttccactt cacggcagcc ccgcggaagt
     1741 cacggcaccc gggccccgcc cgctttgagg gcgaccgcca tttccgcctc taccccgctc
     1801 tccccgcgat ccagcttc
//
LOCUS       DAOQMF010000433         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46406_length_1817_cov_2.762202, whole genome shotgun sequence.
ACCESSION   DAOQMF010000433 DAOQMF010000000
VERSION     DAOQMF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46406_length_1817_cov_2.762202"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PLV77_07525"
     CDS             <1..777
                     /locus_tag="PLV77_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC15733.1"
                     /translation="AWTSKVRLGTGIVGVFQRGPALLAQQAAALTDASSGRFVLGVGA
                     SSDRMVEGWNQIPFVKPLSKVEQTVDFLRVALAGERTDTGFKLETAPTHETPIVLAAL
                     RGKMLELAVNKADGAFTNFLPLAGLPKVVDGIEGAGNDFELLCRFFCIPGEKEQVEGL
                     ARFMFSSYITVPVYANFFRWLGYGEEIEAMVEHWNNKDRQAAAAAAPWELIEDTFLLG
                     SPEQIRARLDEYVAGGITLPVITPVTTPDNHAAMIKALAP"
     gene            complement(875..1777)
                     /locus_tag="PLV77_07530"
     CDS             complement(875..1777)
                     /locus_tag="PLV77_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15734.1"
                     /translation="MGVRLKSTRGQSTIEWVGLITLVAALMAALTAAGIQVPAVGLAH
                     SVSTRLLCAASLSGSCAGEGSLDEAYGPEVAELVRSGAPALMYGKDMLGLPVDYRTCR
                     SPWCADGTGEGEVSRSTAGEPVTLFTRVIHEPDGSIYIQYWAYYPESASLRGAPLLED
                     EGYHRNDWESMQIRVDPDGVVSERASSHAGYNHTRSSVNWGSDLGWGFLTDAAEKVGL
                     REKGGWGDATGQYLIAGGSHAGNVAASLADSDYPSYTPSALVRLVPLESVKDGPLARP
                     ADFDPITPPWEKEVWTEPEAEGTG"
BASE COUNT          309 a          639 c          575 g          294 t
ORIGIN      
        1 gcctggacca gcaaggtgcg gctcggcacc ggcatcgtcg gggtgttcca gcgcggaccg
       61 gccctgctcg cccagcaggc cgccgccctc accgacgctt cctccggccg cttcgtcctc
      121 ggcgtcggcg cttcctccga tcgcatggtc gagggctgga accagatccc cttcgtcaag
      181 ccgctttcca aggtcgaaca aaccgtggac ttcctgcggg tagccctcgc cggggagcgc
      241 accgacaccg gcttcaaact ggagacggcc ccgacccacg aaaccccgat cgtgctggcc
      301 gccctgcgcg gcaagatgct cgagctggcg gtcaacaagg ccgacggcgc cttcaccaac
      361 ttcctgcccc tggccggcct gccgaaggtg gtcgacggca tcgagggcgc cggcaacgac
      421 ttcgagctgc tttgccgttt cttctgcatc cccggtgaaa aggaacaggt cgagggcctc
      481 gcccggttca tgttctccag ctacatcacg gtgccggttt acgcgaactt cttccgctgg
      541 ctcggctacg gcgaagagat cgaagcgatg gtcgagcact ggaacaacaa ggaccgccag
      601 gccgccgcgg cggccgcgcc ctgggagctg atcgaagaca ccttcctgct cggcagcccg
      661 gagcagatcc gcgcgaggct cgatgaatac gtcgccggcg ggatcaccct gcccgtgatc
      721 accccggtca ccaccccgga caatcacgcc gcgatgatca aggccctggc tccctgaagc
      781 cttcctccaa tcgagaaggc gaaagtggcc acatagcgct ccattcagcc ttctcggctg
      841 ctcccgacat gcgggcagct cgaaagccag ccggctagcc ggttccctcg gcttcgggtt
      901 cggtccagac ctccttctcc cagggcggcg tgatcggatc gaagtcggcc ggtcgtgcga
      961 gcggaccgtc cttgaccgat tccagcggga ccagccggac cagggcggac ggagtgtagg
     1021 aggggtagtc cgaatcggcg aggctggcgg caacgttgcc ggcgtggctg ccgccggcga
     1081 tcaggtactg cccggtcgca tcaccccagc ctcccttctc gcgcaggcca accttctctg
     1141 ccgcgtcggt cagaaagccc cagccgaggt cggagcccca gttgaccgac gaccgcgtgt
     1201 ggttgtagcc ggcatgggag gaagcccgtt cgctgaccac tccgtcggga tcgaccctga
     1261 tctgcattga ttcccagtca ttccggtggt agccctcgtc ctcgaggagc ggtgcccctc
     1321 gaagcgaggc gctctcgggg tagtaggccc agtactggat gtagatgctg ccgtcgggct
     1381 cgtggatgac cctggtgaaa agcgttaccg gctctccggc agtggaccgg ctgacctcgc
     1441 cctccccggt tccgtcggcg caccagggcg atcggcaggt ccggtaatcg accggcaggc
     1501 cgagcatgtc ctttccgtac ataagggccg gcgccccgga acgaacgagt tcagcgactt
     1561 ccggaccgta ggcctcgtcg agcgaaccct cgccggcgca ggaaccggac aacgaggcgg
     1621 cgcagagcaa cctggtcgag acggaatggg caagcccgac cgccggcacc tggattcccg
     1681 ccgcggtgag cgccgccatc agggccgcga ccagggtgat caacccgacc cactcgatcg
     1741 tcgattggcc ccgcgtcgat ttcagcctca cgcccatcgc ccaatcgtgg cgggaaaaag
     1801 ctcacaagtg tgttacc
//
LOCUS       DAOQMF010000434         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46439_length_1816_cov_3.522430, whole genome shotgun sequence.
ACCESSION   DAOQMF010000434 DAOQMF010000000
VERSION     DAOQMF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46439_length_1816_cov_3.522430"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1816)
                     /gene="secD"
                     /locus_tag="PLV77_07535"
     CDS             complement(<1..>1816)
                     /gene="secD"
                     /locus_tag="PLV77_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNC15735.1"
                     /translation="VGPARDLSLVGTGADTAEVSKQVRKAWVDAGRPPTEGFNRTWIG
                     LGAEPTMWDAVNLAAKQPRDDKCEDCTSGDKYYLFDEKTHELISGPVTAKEDLYYNDQ
                     GRPIPHKGIVKKVPQGTIIVDELPTDSDGKTIDDESQAGYFVLKDNAALSGSEIKDPQ
                     QNLDQFNQPNVTFDFTGDGKDSFQEITRRIAQEGQARAIGGVTSADQASALSGHFAII
                     LDGQVVSRPIINFVENPDGIDGRQGAQISGGFTVDQAQNLAELLQRGALPIDLKLTSQ
                     SQVSATLGQEALDQGVLALLVGLFCVIIFLIVFYRFLGGVASLALLVYAVLFLALIKV
                     IPITMTLPGIAGLVLTIGVAADSNIVIFERVKEELRAGRSVQSAITLGYRRGISTIID
                     ANVVTLLTAFILFVLATSGIKGFAFTLGVGTIVSLLTAVVFTQALLGSMSNTKLMRSD
                     KAFAGMEGKERWHFDFMGSAPKFFAISGVILLIGAGALTTKGLNFGIDFESGTRIEVA
                     LNDSASTEDVRSVLSPLGLGSADIQTITDNPTLGDNAFQIESETLDPSEVSKVDDALN
                     KEFGVSADGFNSTSVGPTFGASVARSAALAIFFSMILIM"
BASE COUNT          318 a          539 c          626 g          333 t
ORIGIN      
        1 ccatgatcag gatcatcgag aagaagatcg caagggccgc gctgcgggcg accgaggcgc
       61 cgaacgtcgg accgaccgag gtgctgttga agccgtcggc acttacgccg aactccttgt
      121 tcagcgcgtc gtcgaccttc gagacctcgg acgggtcgag ggtctcgctc tcgatctgga
      181 aggcgttgtc accgagggtc gggttgtcgg tgatggtctg gatgtcggcg ctgccgagcc
      241 cgagcgggct gagcacgctg cggacatcct cggttgaggc cgaatcgttc agggccacct
      301 cgatccgggt gccggactcg aagtcgatgc cgaagttgag gcccttggtg gtcagcgcgc
      361 cggcaccgat cagcaggatc acgccggaga tcgcaaagaa cttcggcgcg ctgcccatga
      421 agtcgaaatg ccatctctcc ttgccctcca tgccggcgaa tgccttgtcg gagcgcatca
      481 gcttcgtgtt cgacatcgaa ccgagcagcg cctgggtgaa gacgaccgcg gtcaggagcg
      541 aaacgatggt gccgacaccg agcgtgaagg cgaagccctt gatgcccgag gtcgcgagga
      601 cgaagaggat gaaggcggtc agcagggtga cgacgttggc gtcgatgatc gtgctgatcc
      661 cgcgccggta accgagcgta atcgccgatt gcacggatct tcctgcacgc aactcttcct
      721 tgacgcgttc gaagatgacg atgttggagt cggctgcgac cccgatggtt aagaccagac
      781 cggctatgcc cggcagggtc atggtgatcg ggatcacctt gatcagggcg aggaagagga
      841 ccgcgtagac cagcagcgcc agcgaggcga caccgccgag gaagcggtag aagacgatca
      901 ggaagatgat cacgcagaag agcccgacca gcagcgccag cacgccctga tcgagagctt
      961 cctgcccgag ggtcgccgac acctggctct gcgaagtcag cttgaggtca atcggcaggg
     1021 caccacgctg caggagctcg gccaggttct gcgcctggtc gaccgtgaac ccgccggaga
     1081 tctgggcccc ctggcggccg tcgatgccgt ccgggttctc gacgaagttg atgatcggcc
     1141 tcgagacgac ctggccgtcg aggatgatcg cgaagtgacc ggaaagggcc gaagcctggt
     1201 cggccgaggt gacccctccg atcgcgcggg cctgcccttc ctgggcgatc cggcgggtga
     1261 tctcctggaa ggagtccttg ccgtcgccgg tgaagtcgaa ggtcacattc ggctggttga
     1321 actggtcgag gttctgctgg ggatccttga tctcggatcc ggaaagggcc gcattgtcct
     1381 tgaggacgaa gtagccggcc tgcgactcgt cgtcgatcgt cttgccgtcg gaatcggtcg
     1441 gcagctcgtc gacgatgatc gtgccctggg gaaccttctt gacgatgccc ttgtgaggga
     1501 tcggccggcc ctgatcgttg tagtagaggt cttccttcgc cgtgaccggt cccgagatca
     1561 gttcatgggt cttctcgtcg aagaggtagt acttgtcacc ggaggtgcag tcctcgcact
     1621 tgtcgtcgcg tggctgcttg gcggcgaggt tgacggcgtc ccacatcgtc ggttcggcac
     1681 cgaggccgat ccaggtgcgg ttgaagcctt cggtcggcgg gcggccggcg tcgacccagg
     1741 ccttgcggac ctgcttcgag acttctgcgg tgtcggctcc cgtcccgacc aggctgaggt
     1801 cacgggccgg tccgac
//
LOCUS       DAOQMF010000435         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46462_length_1816_cov_2.260647, whole genome shotgun sequence.
ACCESSION   DAOQMF010000435 DAOQMF010000000
VERSION     DAOQMF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46462_length_1816_cov_2.260647"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLV77_07540"
     CDS             <1..303
                     /locus_tag="PLV77_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15736.1"
                     /translation="VEAVCGIAALMLAGLLCFQLLATGYTATIADGAAEAGAVALIRG
                     EPVEPAVLEALPGWARDRVSVSRVGSRVKVRLRPPSLFSSMSEKLAVSAEAGGRPG"
     gene            300..>1816
                     /locus_tag="PLV77_07545"
     CDS             300..>1816
                     /locus_tag="PLV77_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNC15737.1"
                     /translation="MSEHLLIGSIGDSRGAERLAAERVLESAAGRVGVVLETGRERPR
                     PASLLAPGEAKELESELLEALPELEPKARGSLVYLSAAQSERLAGEVISLREIVDERP
                     LAVIAGAGRYRELLELGWDRVVIRSDEAGQDAPEVDAPVSGDAVAGPGALTGLVVEEA
                     GEFADRVELVRGAAGKLWPAGGRLLGRLRSTGGQATPLVLGCVFALVLATVAVVAISG
                     AVTGKARAQRAADLSALSAVRSMKDDLPRLLAPPRLPNGLPNPAHMPKQVYLARARLA
                     AIGAATSNGASPLAVSVRFPDSASYAPVRARVSIATSVRPGTGGGAHRAPPNWAEARV
                     SVPVSMGSVPAMASGGGYSGPLAERQGHGMRPDVAAAFDAMVASAAAAGVSLTINSAF
                     RSDAEQAALFAANPDPTWVAPPGQSLHRCATELDLGASDGYSWLAANAGRFGFVQRYS
                     WEAWHYGFVAGPAPCSDAGNSVGMDGGDRSAALSRAVPSFTPGWVRPLVLRAAMK"
BASE COUNT          219 a          613 c          697 g          287 t
ORIGIN      
        1 gtcgaggcgg tctgcggcat cgccgccctg atgctggccg gcctgctctg cttccagctg
       61 cttgccaccg gttacaccgc gaccatcgcc gacggggcgg ccgaggctgg cgcggtcgcg
      121 ctgatccggg gcgagccggt cgagccggcc gtgctagagg cattgcccgg ctgggcccgg
      181 gaccgggtct cggtgtcgag ggtcgggtcc cgggtaaagg tccggctccg gccgccttcg
      241 ctcttctcct cgatgtccga aaagcttgcg gtttcggccg aggcgggcgg gaggccggga
      301 tgagcgagca tctgctgatc gggtcgatcg gcgattcgcg tggcgccgaa cggctcgccg
      361 ccgaacgggt tctggaatcg gcggccgggc gggtcggcgt ggtgctggag accggtcggg
      421 agcggccccg gccggccagc ctgctggctc ccggcgaggc caaggagctg gaatcggaac
      481 tgctggaggc cctgccggag cttgagccga aagcccgcgg gagcctcgtc tacctgtcag
      541 ccgcccagtc agagcggctg gccggggaag tgatcagctt gcgggagatc gtggatgaac
      601 ggcccctggc cgtgattgcg ggggccggcc ggtaccgcga gctgctggaa ctcggctggg
      661 accgggtggt gatcagatcc gacgaggccg gacaggatgc ccccgaggtc gatgcgccgg
      721 tttccggaga tgccgtcgcc ggccccggcg ccctgaccgg gctggtcgtc gaagaggcag
      781 gcgagttcgc cgatcgggtc gaactcgtgc ggggcgcggc cgggaaactg tggcccgccg
      841 gcgggcggct cctgggccgg ctccgttcga ccggcgggca ggcgaccccg ctggtgcttg
      901 gatgcgtctt cgcactggtg ctggcgaccg tggcggtggt cgcgatttcc ggggcggtga
      961 ccggcaaggc aagggctcag cgggcagccg atctctcggc cctttccgca gtccgttcga
     1021 tgaaggatga cctgccgcgg ctgcttgctc cgccgcgcct gccgaacgga ctgccgaacc
     1081 cggcccacat gccgaagcag gtctacctcg cgcgggcgcg gctggcggcg atcggggcgg
     1141 cgacatcgaa cggggcttcg ccgctcgcgg tttcggtccg ttttcccgac tccgcctcct
     1201 atgccccggt gcgggcccgg gtttcgatcg ccacctcggt ccggcccgga accggtggcg
     1261 gggcacaccg ggcaccgccg aactgggccg aggcccgggt cagcgttccg gtctcgatgg
     1321 ggtcggtgcc ggcgatggcc agcggcggcg gctactccgg gccgctggcc gaacggcagg
     1381 gtcacggcat gcggccggac gtggccgccg cctttgacgc gatggtcgcg tccgccgcgg
     1441 cagccggagt aagcctgacc atcaattcgg cctttcgttc ggacgcggaa caggcagccc
     1501 tctttgcggc caacccggat ccgacctggg tcgcgccgcc cggccagtcg cttcaccgct
     1561 gcgcgaccga actcgatctc ggtgcctccg acggctacag ctggctggca gccaacgccg
     1621 ggcgtttcgg cttcgtccag agatacagct gggaagcctg gcattacggg ttcgtcgccg
     1681 gcccggcgcc atgctcggat gcgggaaact cggtcggcat ggacgggggc gaccgttcgg
     1741 cggcgctctc gcgggcggtg ccgtcattca ccccgggctg ggtccggccc ctggtccttc
     1801 gcgcagccat gaagtg
//
LOCUS       DAOQMF010000436         1815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46531_length_1815_cov_2.052841, whole genome shotgun sequence.
ACCESSION   DAOQMF010000436 DAOQMF010000000
VERSION     DAOQMF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46531_length_1815_cov_2.052841"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLV77_07550"
     CDS             <1..894
                     /locus_tag="PLV77_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC15738.1"
                     /translation="GPYGDSVAGPAEIVEVLIAGINPVDLAIASGKFDAGAPPLPYTP
                     GLEGIGRMADGEVVWFEGAHFPWGSMAERAVITEGSGITLPPGIEPEQAIAFGTAGMA
                     AWLSTDWRGQLKDGETVLVLGASGSVGQVAVQAARMMGAGRVVGMARSEAGRERVLAL
                     GADAAIASDGSVEELKARIFEATEGGQDLVIDGLWGLPAAAALQTMKPHGRLVQVGNS
                     AGTEAPIAAGPVRGAAAAILGHRNFWPPREVRVATFERMCSLAASGDLELEVEVLPLE
                     EVAEAWDRQSRSPGHKLALRP"
     gene            complement(908..>1815)
                     /locus_tag="PLV77_07555"
     CDS             complement(908..>1815)
                     /locus_tag="PLV77_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC15739.1"
                     /translation="YRRRPTPDRGGPADVVRAMAVTMQEAALDAGTEAHRLLAVGVGS
                     PGSVDPDSGAVSGAGNLPDWDGTFQLRLALQEELGTEVRVGNDVQVGTRGEYELGAGA
                     GYSSLLGVFWGTGVGGGIIINGEPWLGRGRAGEIGHTLVRRGGAKGLNGLNGTVEAYA
                     GRAAMEEKARREVKKGRKTKLFKLMKKHEKTHLTSAIWQHALDQDDELAQDLLNRAVG
                     ALSAGIASAVNLLDPEAVVIGGGLGVRFADTLVPEIIAGMDQYLLNKDNPPAVKVAAL
                     GDEGGVIGASLLVKELVEANQAPTA"
BASE COUNT          262 a          622 c          644 g          287 t
ORIGIN      
        1 ggtccctacg gcgactcggt ggccggaccg gcggagatcg tcgaagtcct gatcgccggg
       61 atcaacccgg tcgacctcgc gatcgcctcc ggaaagtttg acgccggcgc cccgccgctt
      121 ccgtacacac ccggcctcga agggatcggc cggatggcgg acggggaggt cgtctggttc
      181 gaaggggcgc atttcccctg gggttcgatg gctgaacggg cagtgatcac cgaaggcagc
      241 ggcatcacgc ttcccccggg gatcgaaccc gaacaggcga ttgccttcgg gaccgccgga
      301 atggcggcct ggctttcgac cgactggcgc gggcagctca aggatggcga gaccgtgctg
      361 gtcctcggag ccagcggctc ggtcggccag gtagcggttc aggcggcccg gatgatgggg
      421 gccggccgcg tggtcggcat ggcccgcagc gaagccggcc gcgagagggt tctcgcgctg
      481 ggcgcggatg cggcgatcgc ctccgacgga agcgtcgagg agctgaaggc ccggatcttc
      541 gaggcgaccg aaggcgggca ggacctggtg atcgacggct tgtggggcct gcccgccgcc
      601 gcggctctcc agaccatgaa gccgcatggc aggctggtcc aggtcggcaa ctccgccggg
      661 accgaagcgc cgatagccgc cgggccggtg cggggagcag ccgcggcgat tctcggccac
      721 cgcaatttct ggccgccgcg ggaggtgcgg gtcgcgacct tcgaacggat gtgttcgctg
      781 gcggccagcg gcgacctcga actcgaggtc gaggttttgc cgctagaaga ggtggccgag
      841 gcctgggacc gtcagtcccg aagccccggc cacaaactcg cgctcagacc ctgaggcttg
      901 ccagccgtca ggcggttggt gcctggtttg cctcgacgag ttccttgacc agcagggaag
      961 cgccgatcac gccgccctcg tctccgagcg cggcgacctt tacggccggc ggattgtcct
     1021 tgttcagcag gtactgatcc atgccggcga tgatctccgg gaccagggtg tcggcaaacc
     1081 ggacgccgag cccgccgcca atgaccaccg cttcggggtc gagcaggttg accgccgagg
     1141 cgatccccgc ggaaagggct ccgaccgcac ggttgaggag gtcctgggcc agctcgtcgt
     1201 cctgatcaag tgcgtgctgc cagatcgccg aagtgaggtg ggtcttctca tgcttcttca
     1261 tcagcttgaa gagcttggtc ttgcggccct tcttgacttc ccggcgggcc ttctcctcca
     1321 tcgccgcgcg accggcatag gcctcgaccg tgccgttgag gccgtttagg cccttggcgc
     1381 caccgcggcg aaccagcgtg tgaccgatct ctccggcccg gccgcggccg agccacggct
     1441 cgccgttgat gatgatcccg cccccgactc cggtgcccca gaagaccccg agaaggctgg
     1501 agtagcccgc cccggcgccg agctcgtatt cgccgcgggt gccgacctgg acgtcgttgc
     1561 cgacccggac ttccgttccc agttcttcct ggagtgccag tcgcagctgg aaggtgccgt
     1621 cccagtccgg caggttcccg gcgccactca cggcgccgga atcggggtcg accgacccgg
     1681 gcgatcccac cccgacggcg agcagccgat gggcctcggt tccggcgtcg agcgcggctt
     1741 cctgcatggt caccgccatc gcccgcacca cgtcggcggg gccgccgcga tccggagtcg
     1801 gacgccggcg gtacg
//
LOCUS       DAOQMF010000437         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46707_length_1811_cov_4.306378, whole genome shotgun sequence.
ACCESSION   DAOQMF010000437 DAOQMF010000000
VERSION     DAOQMF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46707_length_1811_cov_4.306378"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLV77_07560"
     CDS             <1..302
                     /locus_tag="PLV77_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15740.1"
                     /translation="DGLRRFLDPAFLLQRGDGSYLTKSEYLKDPSEVFAFKVRNIVAT
                     HNGDVRVLRLEAKTDQIVNGNPVPGVWIPRLSTFKKTGKVWRLIAHANFLPPVAT"
     gene            complement(446..518)
                     /locus_tag="PLV77_07565"
     tRNA            complement(446..518)
                     /locus_tag="PLV77_07565"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Ala,seq:ggc)
     gene            complement(544..930)
                     /gene="rsfS"
                     /locus_tag="PLV77_07570"
     CDS             complement(544..930)
                     /gene="rsfS"
                     /locus_tag="PLV77_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNC15741.1"
                     /translation="MAASSEELSRDIARLVDEKLGEDIVCLDMRELVTYTDYLVICTA
                     RNERQAEAIADEVHLRLKHDNSLLPVNPDRSGDVGWTVLDYLDCVLHIFTEEARQRYD
                     LEDLWHEAPHLDLGLDQSGAKSASAA"
     gene            complement(914..1519)
                     /gene="nadD"
                     /locus_tag="PLV77_07575"
     CDS             complement(914..1519)
                     /gene="nadD"
                     /locus_tag="PLV77_07575"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNC15742.1"
                     /translation="MTRLGVFGSSFNPPTLAHAVLLAEAGWQLALDRIMVVPTGEAWH
                     KDISGAPAAGVRMALAKAAFGGQEGLEVSSLEVDRQGPSYTCDTLEEIHSSNPDSQVF
                     FLSGSDAALGLGEWHRPDRVLELAEFAVAPRSEIARDAVAEVFERLGARDRLEFFAMP
                     RMEFSSTLVRERIASNRPWKHLVPQGVAEMIDNEDLYGSKQ"
     gene            complement(1521..>1811)
                     /locus_tag="PLV77_07580"
     CDS             complement(1521..>1811)
                     /locus_tag="PLV77_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC15743.1"
                     /translation="VVDGLDEAIEHINRHGSGHSDAIVTDNAQAADLFAQGVDSAAVY
                     VNASTRFTDGFEFGMGAEIGVSTQKLHARGPVGLKELTTTKYVGRGDGQVRG"
BASE COUNT          322 a          599 c          573 g          317 t
ORIGIN      
        1 ccgacgggct ccgtcgtttc ctcgaccccg ctttcctcct ccagcgcggt gacggcagtt
       61 acctgaccaa gtccgaatac ctcaaggatc cttcggaagt cttcgccttc aaggtccgca
      121 acatcgtcgc cacccacaac ggtgacgtga gggtgcttcg cttagaggcg aagaccgacc
      181 agatcgtcaa cggcaacccg gttccggggg tttggatccc ccggctttcg accttcaaga
      241 agaccggcaa ggtctggcgc ctgatcgccc acgccaactt cctcccgccc gtcgcgacct
      301 agaggaacag ccgaggtgcc gcaaaatcag ccccgtcacg gggctggatg tgcggcacct
      361 ttgcgcgtgg aagcccgtgg agccgcgcga acggctccgt ggggaccgcc cgccgtacgc
      421 ggggacttcg aggagaactt acgagtggag ctacccggga tcgaaccggg gacctcttgc
      481 atgccatgca agcgctctgc cagctgagct atagccccag agggacgaag gagtttaccg
      541 ggatcaggcg gccgaggcgg acttcgcccc ggactggtcg agaccgagat cgaggtgggg
      601 cgcttcgtgc cagaggtctt cgaggtcgta gcgctgcctg gcttcctcgg tgaagatgtg
      661 gaggacgcag tcgaggtagt cgagcacggt ccatccgacg tcaccggagc ggtccggatt
      721 gaccggcagc agcgagttgt cgtgcttgag gcgcaggtgg acctcgtcgg caatcgcctc
      781 ggcctgccgt tcgttgcggg cggtgcagat caccaggtag tcggtgtagg tgaccagctc
      841 gcgcatgtcg agacagacga tgtcctcgcc gagcttttca tccaccagcc gggcgatatc
      901 ccggctcagt tcttcactgc ttgctgccat acagatcctc attgtcgatc atttccgcca
      961 ccccctgcgg tacgaggtgt ttccagggcc ggttcgaggc gatccgctcc cggaccaaag
     1021 tcgatgaaaa ctccatcctc ggcatcgcga agaactcgag ccggtctcgc gctccgagcc
     1081 tctcgaagac ctccgccacc gcgtcccggg cgatctctga acgcggtgcc acggcgaatt
     1141 ccgccagctc cagcacgcgg tcgggccgat gccactcccc caaccccagg gcggcatcag
     1201 agcccgagag gaagaagacc tggctgtccg gattcgaaga atggatctcc tcaagggtgt
     1261 cacatgtgta ggacggaccc tgccggtcca cttcgagcga tgaaacttca agcccttcct
     1321 ggccgccgaa ggctgccttc gccagggcca tccggacccc ggcggcgggc gccccggaaa
     1381 tgtccttgtg ccaggcctcc ccggtcggca cgaccatgat ccggtcaagg gcgagctgcc
     1441 agccggcttc ggcgagcagc accgcgtggg ccagcgtcgg cggattgaaa gaagagccga
     1501 aaaccccgag ccgggtcact tcagccgcga acctggccgt cgccgcggcc cacgtacttg
     1561 gtcgtggtca gttccttgag gccgaccggc ccgcgggcgt gaagcttctg ggtcgaaacg
     1621 ccgatttcgg ctcccatacc gaattcgaat ccgtcggtga accgggtcga agcgttcacg
     1681 tagacggcag ccgaatcgac accctgggca aaaagatcgg ccgcctgggc gttgtcggtg
     1741 acgatcgcgt cggagtgtcc gctgccgtgg cggttgatgt gctcgatcgc ttcgtcgagc
     1801 ccgtccacca c
//
LOCUS       DAOQMF010000438         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46764_length_1810_cov_3.574929, whole genome shotgun sequence.
ACCESSION   DAOQMF010000438 DAOQMF010000000
VERSION     DAOQMF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46764_length_1810_cov_3.574929"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLV77_07585"
     CDS             <1..206
                     /locus_tag="PLV77_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15744.1"
                     /translation="LVPDDAVGPVIETAINLVSAIFPFRPTLDAITGGLEISGPTLWL
                     PLLHLVILTAAYGALARLALRRF"
     gene            322..1299
                     /gene="hemB"
                     /locus_tag="PLV77_07590"
     CDS             322..1299
                     /gene="hemB"
                     /locus_tag="PLV77_07590"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNC15745.1"
                     /translation="MSFPSTRLRRLRRTPALRGLVRETGLSAADLIQPLFVTAGTDLR
                     EPVESMPGVNRFSIAALVEEAGEIQAAGIPAVLVFGVPSEKDEAGSGAYDDEGVVQMA
                     VRALKESHPDLIVITDVCLCEYTDHGQCGFVRDGQVDNDVTVELLARTAISHAEAGAD
                     IVAPSDMMDGRIGSIRYQLDEEGHPDTAILSYAAKYASAFYGPFREAAESTPAFGDRR
                     GYQMDPANSDEAVREAKLDLEEGADALMVKPAGHYLDIVRRVKDETGAPVAAYQVSGE
                     YSAIRAAGANGWIDERAAALESLVSIRRAGADFIVTYFAREAAAWLAES"
     gene            1325..>1810
                     /locus_tag="PLV77_07595"
     CDS             1325..>1810
                     /locus_tag="PLV77_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC15746.1"
                     /translation="MSGLSRGAPDKVTIKVRGDGSPLAETGSEVEYREEEKAKAAAAQ
                     AAAGRAGNEELFERARRVLPGGVNSPVRAMGQVGIDPVFIESARGAEVLDRQGRRYLD
                     WVSSWGPMILGHADPEVLAAIEEAAGRGTSFGAATELEVELAEEVAERMPSVEMIRMT
                     SS"
BASE COUNT          300 a          602 c          631 g          277 t
ORIGIN      
        1 ccctggttcc cgacgacgcg gtcggcccgg tgatcgaaac cgcgatcaac ctggtcagcg
       61 cgatcttccc cttccgccca accctcgacg cgatcaccgg cggcctcgag atctccggtc
      121 caaccctctg gctccccctc ctccacctgg tcatcctcac cgccgcctac ggagcgctgg
      181 cccgcctcgc cctacgccgt ttttgactta actgccagtc gggcggggtc cgacccgccc
      241 tccacgagga acccgcccct cccattcgag acaactgatg gttgggaggg gctcagcctg
      301 aagtacttct agactcgact tatgagcttt ccgagtaccc gcctgcgccg tctgcgccgc
      361 accccggccc tccgcgggct ggttcgtgaa accggcctgt cggcggccga cctgatccag
      421 ccgctcttcg tcacggccgg caccgacctg cgcgaaccgg tcgagtcgat gccgggggtc
      481 aaccggttct cgatcgccgc cctggttgag gaggcggggg agattcaggc ggcggggatt
      541 ccggcggtgc tggtcttcgg ggttccctcg gagaaggacg aggcgggcag cggggcctac
      601 gacgacgagg gcgtggtcca gatggcggtg cgggccctga aggagagcca cccggacctg
      661 atcgtgatca ccgacgtctg cctttgcgaa tacacggatc acggccagtg cggatttgtc
      721 cgcgacgggc aggtcgacaa cgacgtgacg gtcgagttgc tggcgcggac cgcaatttcc
      781 catgcggagg ccggggccga catcgtcgcg ccgagcgaca tgatggacgg ccggatcggg
      841 tcgatccgct accagctcga cgaggaaggc caccccgaca cggcgatcct ctcctacgcc
      901 gccaagtacg cgtcggcgtt ctacggaccg ttccgcgagg cggccgaatc aacccccgct
      961 ttcggtgatc gtcgcggcta ccagatggac ccggccaact cggacgaagc ggtccgcgag
     1021 gcgaagctcg acctcgagga gggcgccgac gcgctgatgg tcaaacccgc cggccactac
     1081 ctcgacatcg tccgccgggt caaggacgag accggcgccc cggtcgcggc ctatcaggtg
     1141 tccggcgagt actcggcgat cagggcggcc ggggccaacg gctggatcga cgagcgggcc
     1201 gcggcccttg aatcactggt ctcgatccgg cgggccggag cagatttcat cgtcacctac
     1261 ttcgccaggg aagcagccgc gtggctggct gaatcgtgag gagtttcatt gtgaggagaa
     1321 gcggatgtca ggtctgagcc ggggtgcccc cgacaaggtc acgatcaagg tgcgcggcga
     1381 cggcagcccg ctggccgaga ccggttccga ggtcgaatac cgggaagagg agaaggcgaa
     1441 agccgcggcg gcacaggcgg ctgccggacg ggccggcaac gaggagctat tcgagcgggc
     1501 ccgccgggtc ctgccgggag gggtgaattc accggtccgg gcgatgggcc aggtcgggat
     1561 cgaccccgtc ttcatcgaat ccgccagagg cgcggaagtg ctcgaccggc aaggccgccg
     1621 ctatctcgac tgggtgagtt cctgggggcc gatgatcctc ggccacgccg accccgaggt
     1681 cctggcggcg atcgaggagg cggccggacg gggaacgagc tttggcgcgg caaccgagct
     1741 cgaggtggaa ctggccgaag aggtggcaga gcggatgcct tcggtcgaga tgatccgcat
     1801 gaccagttcg
//
LOCUS       DAOQMF010000439         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46773_length_1810_cov_3.152137, whole genome shotgun sequence.
ACCESSION   DAOQMF010000439 DAOQMF010000000
VERSION     DAOQMF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46773_length_1810_cov_3.152137"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLV77_07600"
     CDS             complement(<1..484)
                     /locus_tag="PLV77_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC15747.1"
                     /translation="MSVKRIGIASLLAMFAALVVPGMASAAPVTQELFDAKSAELNGT
                     FIALACVLVLLMQCGFMFLEIGFSRMKNAGAGVMKIFVNFSVCTLAFWAVGAAIIGYG
                     NDFIGTHGFFYHFGSMVDDGSGTGTLVAVDSSAVMYMLYSFAFCAVSLAIVWGTTLER
                     I"
     gene            complement(779..1654)
                     /locus_tag="PLV77_07605"
     CDS             complement(779..1654)
                     /locus_tag="PLV77_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC15748.1"
                     /translation="MNRPAAPTPEAVLEWAGTVQSTSDLILRRLAGEYHEDAWGFDEE
                     FTEAVFPLFEFLYSTWWRVQVEGAANVPGHGRALLVANHSGAIFPFDGAMIATAIQKE
                     HPLPRWVKAMVLNWAFDLPFVSYILRRLGGVAANPANLGRLLEEDQPVMVFPEGQKGF
                     GKSFADRYRLQRFGRGGFVEVALETGSPIVPVAVVGAEEIYPKLGESKPLARAIGSPY
                     VPVTPTFPWLGPLGLLPLPSRWRIEFLEPIDLSGYGPGSSEDRSLVFDLSEQVREQIQ
                     EALYRGLVKRGPAFL"
BASE COUNT          346 a          574 c          605 g          285 t
ORIGIN      
        1 tgatgcgctc cagggtggtg ccccagacga tggcgagcga gaccgcgcag aacgcgaagc
       61 tgtacagcat gtacatgacc gcgctcgaat cgacggcgac cagcgtgcct gtgccgctgc
      121 cgtcatcgac catcgaaccg aagtggtaga agaacccgtg ggttccgatg aagtcgttgc
      181 cgtaaccgat gattgcggcc ccgaccgccc agaaggcgag ggtgcagacc gagaagttga
      241 cgaagatctt catcactccg gcgcctgcgt tcttcatccg ggagaaaccg atctccagga
      301 acatgaagcc gcactgcatg agcagcacga gcacgcaggc gagcgcgatg aaggttccgt
      361 tcaactcggc cgacttggcg tcgaacagtt cctgtgtgac cggcgccgca ctcgccatcc
      421 ctggcaccac cagggcggca aacatcgcca gaagcgaggc tatgccgatt ctcttgacac
      481 tcatacgagt cattcctcct gtttccgccg ggcccagacc caccggttgc aaatagcgtt
      541 tagctgaccg ggccaattta gaaacgggag ggggagcgct ttttggccac tcggaggaaa
      601 tgaccccatc ccggttgttc gattgttaaa tcgaaggtaa atggctctac agagccgagt
      661 cgtagttgag attcgggtct gtgagcggac tcgggcgact ggatcggcgg ccgttttgtc
      721 cgccgggaca cccggccagg caactgggcc gccggccccc ggacttcacg gccgcgggct
      781 agaggaatgc ggggccgcgt ttgaccaggc cgcgatacag ggcttcctgg atctgctccc
      841 gcacctgctc ggagagatcg aatacgagcg agcggtcctc gctcgaaccc ggtccgtacc
      901 cggagaggtc gatcggctcg aggaactcga tccgccagcg cgagggcaaa ggcagcaggc
      961 cgagcggtcc cagccagggg aaggtcgggg taaccggcac gtagggagaa ccgatcgctc
     1021 gcgccagcgg ctttgattcg ccgagtttcg gatagatctc ttcggccccg accacggcga
     1081 ccgggacgat cggcgacccg gtctcgagcg caacctcgac gaacccgccg cggccgaagc
     1141 gctgcagccg gtaacggtcg gcgaatgact ttccgaagcc cttctggcct tcggggaaga
     1201 ccatcaccgg ctgatcctcc tccagcagcc gaccgaggtt tgccgggttg gcggccaccc
     1261 cgccgagccg gcgcaggatg taactgacaa agggcaggtc gaaggcccag ttgagcacca
     1321 tcgccttgac ccagcgaggc aaggggtgct ccttctggat cgcggtggcg atcatcgcgc
     1381 cgtcaaaggg gaagatggcg ccggaatggt tggcgaccag cagggcccgg ccgtgccccg
     1441 gcacgttggc cgccccttca acctggaccc gccaccaggt cgagtagagg aattcgaaga
     1501 gcgggaagac cgcttcggtg aactcttcgt cgaagcccca ggcatcctcg tggtactcgc
     1561 cggccagccg gcggaggatc agatccgagg tggactggac cgttccggcc cactcgagca
     1621 cggcctccgg ggtcggggcg gcggggcggt tcaccgggac acgctcctga ccggagaacc
     1681 cggatggggt gacggaaaga gggcctggat gccctgcgag gtgcgggcga agtcgcgggc
     1741 cgttccctcc gagtcgaagg cgagctcgaa cccgacctcc tcccgcagcc tgcggttgtc
     1801 gcagccgcgg
//
LOCUS       DAOQMF010000440         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_46818_length_1809_cov_3.166477, whole genome shotgun sequence.
ACCESSION   DAOQMF010000440 DAOQMF010000000
VERSION     DAOQMF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46818_length_1809_cov_3.166477"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLV77_07610"
     CDS             <1..669
                     /locus_tag="PLV77_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNC15749.1"
                     /translation="NTTGRLPVGDPEQIPDPAEVEVPEDLREQIQAIVDKYPDRDHKG
                     SPKSAAIPVLWIIQRRYGWCTPEGIVQAACVMQVTPAYLESVASFYDLLRTSPQGSHQ
                     VLVCTNLACWMRGGDELLDSFCEKTGADREAAGHGGSLSEDGEILVSGFECLGACDLA
                     PMASVDERYFGPLENEDAATIVDQLESGEEVLPEKALDKRGLAGGVADSTDPRVTGGK
                     GPNQ"
     gene            666..>1809
                     /gene="nuoF"
                     /locus_tag="PLV77_07615"
     CDS             666..>1809
                     /gene="nuoF"
                     /locus_tag="PLV77_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNC15750.1"
                     /translation="MSVAETRVLLRHAEDPKMATLEGYESYGGYATLKKALNEIDPEW
                     IVTELEESGLRGRGGAGFSMGKKVSFLPRTEDAKYLCCNADESEPGAFKDRELMQRNP
                     HQLIEGIIISSFGANIGHAFIFIRGEYDDQADILDRAVDEAYEAGYLGTNILGSGFNL
                     ELVVHRGAGAYICGEETALLDALEGKRGNPRLKPPFPAVQGLYGGPTLINNVETLSNV
                     SHIVANGADWFKSFGTEQSPGTKVVSVSGCVKRPGNYEVELGVTLRDLIYGLAGGPLD
                     GHEITAFYPGGSSSPVLTPAELDLPYSFEAMAEAGSMLGSGSIIVADDSVSIPRMALR
                     TAHFYRHESCGKCTPCREGTNWTEKMLERVCAGEATPMDLDIIESVQ"
BASE COUNT          327 a          616 c          575 g          291 t
ORIGIN      
        1 aacaccaccg gacgcctccc ggtcggtgat ccggaacaga ttccggaccc ggccgaagtc
       61 gaggtgccgg aggacctgcg cgagcaaatt caggcgattg tcgacaagta cccggaccgg
      121 gaccacaagg gctccccgaa gtcggctgcg atcccggtgc tctggatcat ccagcgccgt
      181 tacggctggt gcaccccgga ggggatcgtc caggccgcct gcgtgatgca ggtgaccccg
      241 gcctacctcg aatcggtcgc ctccttttac gaccttctga ggaccagccc ccaggggagc
      301 catcaggttc tcgtctgcac caacctcgcc tgctggatgc ggggcggcga tgagctgctc
      361 gactccttct gcgagaagac cggggcggac cgcgaagcgg ccggccacgg agggtccctc
      421 tccgaagatg gcgagatcct ggtctccggg ttcgaatgcc tcggtgcctg cgacctcgcc
      481 ccgatggcct cggttgacga gcgctacttc ggacctttgg agaacgagga cgcggccacc
      541 atcgtcgatc agctcgaatc cggtgaggaa gtcctgccgg agaaggcgct ggacaagcgc
      601 ggcctggccg gcggcgtcgc cgattcgacc gacccccggg tgaccggcgg aaaggggccg
      661 aatcagtgag cgtcgctgaa accagagtgc tgctccgcca cgccgaagac ccgaagatgg
      721 cgaccctcga gggttacgag tcctacggcg gctacgcgac cctgaagaag gcgctgaacg
      781 agatcgaccc cgagtggatc gtgaccgagc tcgaggaatc gggcctgcgc ggtcgcggcg
      841 gtgccggatt ctcaatgggc aagaaggtgt ccttcctgcc gcgtacagag gatgccaagt
      901 acctctgctg caacgccgac gaatccgagc cgggggcctt caaggatcgc gagctgatgc
      961 agcgaaaccc gcatcagctg atcgaaggca tcatcatctc cagtttcggg gccaacatcg
     1021 gccacgcttt catcttcatc cgcggcgagt acgacgatca ggccgacatc ctcgatcggg
     1081 cggtcgatga ggcctatgag gcgggttacc tcgggaccaa catcctcggt tcgggcttca
     1141 acctcgagct ggtcgtccac cgcggtgccg gggcctacat ctgcggcgag gaaaccgccc
     1201 tgctcgacgc gctggaaggc aagcgcggca acccgcgcct caagcccccc ttccctgcgg
     1261 tccagggcct ctacggcggg ccgaccctga tcaacaacgt cgagaccctg agcaacgtga
     1321 gtcacatcgt cgccaacggc gccgactggt tcaagagctt cggcaccgag cagtcacccg
     1381 gcaccaaggt cgtttcggtt tccggctgcg tcaaacgacc cggcaactac gaggtcgagc
     1441 tcggcgtcac cctgcgcgac ctgatctacg gcctggccgg cggcccgctc gacggccacg
     1501 agatcaccgc cttctacccg ggaggttcct cctcaccggt gctgaccccg gccgaacttg
     1561 acctgcctta ttccttcgag gcgatggccg aggccgggtc gatgctcggc tccgggtcga
     1621 tcatcgtcgc cgacgactcg gtctcgattc cgaggatggc gctccggacc gctcacttct
     1681 accgtcacga atcctgcggc aagtgcacgc cctgccgcga gggcaccaac tggaccgaga
     1741 agatgctcga gcgggtctgt gccggcgagg ccaccccgat ggacctcgac atcatcgaat
     1801 cggtccagc
//
LOCUS       DAOQMF010000441         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47049_length_1804_cov_4.146941, whole genome shotgun sequence.
ACCESSION   DAOQMF010000441 DAOQMF010000000
VERSION     DAOQMF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47049_length_1804_cov_4.146941"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLV77_07620"
     CDS             complement(<1..597)
                     /locus_tag="PLV77_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HNC15751.1"
                     /translation="MFEQMKTPPVVSAEEWDAARMELLVREKEVTRARDALAAERRRM
                     PWLKIEKDYEFEGPEGRVGLIDLFEGRRQLVLYRFFFDPDVSGHPDAGCPGCSFGADQ
                     VADLRHLNARDTTFAHASRGSQENIRRLKEKMGWEQIPWYTVIDDFDRDMGVGEWHGT
                     NAFIRDDDGTVYRTYFIDARGDEHMGSTWAHLDLTALGR"
     gene            complement(600..1067)
                     /locus_tag="PLV77_07625"
     CDS             complement(600..1067)
                     /locus_tag="PLV77_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC15752.1"
                     /translation="MTVSDQSVHLVKVVQAPPEMVYRAFIDPDELVRWMHPDAFTGVS
                     ASNDPRVGGRGELIHALDGEEVGGFNWQYLEVVPGRRLVMDWQFGPAGADPDGHHSRL
                     TIELREAGPGATEVSLTHDRLGDVPPGGPTGVSTGWTQALASLGRHFEAEGDE"
     gene            complement(1064..1405)
                     /locus_tag="PLV77_07630"
     CDS             complement(1064..1405)
                     /locus_tag="PLV77_07630"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC15753.1"
                     /translation="MVEDQKEVDRVFHALSNQTRRGMVRQLSGGECSVGDLARPTRMS
                     FAAASKHVQVLEKAGMVNRRVEGRQHICSLRPDPLREASEWLSFYEKFWAGNLDRLAR
                     VVEADMKKEKK"
     gene            1551..>1804
                     /locus_tag="PLV77_07635"
     CDS             1551..>1804
                     /locus_tag="PLV77_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15754.1"
                     /translation="MRKLVTFVAVLLGLLAVPATASALKFEAAPGTPLAPATSVIDPS
                     PVMPPGFSPTDIATGDFNKDGYGDIAVITDYISVQGPDGP"
BASE COUNT          308 a          616 c          550 g          330 t
ORIGIN      
        1 gcggccgagc gcggtcaggt cgaggtgggc ccaggtgctg cccatgtgct cgtcgccgcg
       61 cgcgtcgatg aagtaggtgc gatagaccgt gccgtcgtca tcacggatga aggcattggt
      121 tccgtgccac tcgccgacac ccatgtcgcg gtcgaagtcg tcgatcaccg tgtaccaggg
      181 gatctgctcc cagcccatct tctccttcag ccgccggatg ttctcctggc tgccccggga
      241 agcatgggcg aaggtcgtgt cccgggcatt gagatgccgg aggtcggcga cctgatcggc
      301 cccgaacgag cagccagggc aaccggcatc ggggtggccg cttacatccg ggtcgaagaa
      361 gaagcggtag agcacaagct ggcggcggcc ttcaaaaagg tcgatcagcc cgaccctgcc
      421 ctcggggcct tcgaactcgt agtccttctc gatcttcagc cagggcatcc gccggcgttc
      481 ggcggccagc gcgtccctgg ccctggtcac ttccttctcg cggaccagca actccatccg
      541 ggccgcatcc cattcctcgg ccgagacgac cggtggcgtc ttcatctgct cgaacatgac
      601 tactcatctc cttctgcttc gaaatgccgc ccgagactcg ccagagcctg ggtccagcct
      661 gtgctgactc ctgtgggtcc gcccggcggc acatcaccaa ggcggtcgtg ggtcagcgaa
      721 acctcggtcg ctcccggccc ggcttcccgc agctcgatcg tcaggcggga gtggtgacca
      781 tcggggtcgg cgccggccgg gccgaactgc cagtccatca ccagacgccg gccgggaacc
      841 acctccaggt actgccagtt gaaaccgccc acctcttccc cgtccagggc gtggatcagc
      901 tcgccccggc cgccgacccg ggggtcgttg ctggccgaga ctccggtgaa ggcatcgggg
      961 tgcatccagc ggaccagttc atccgggtcg atgaacgccc ggtagaccat ttcgggggga
     1021 gcctgaacga ccttgacgag atgaacggat tgatcagaga cagtcatttc ttctccttct
     1081 tcatatccgc ttccactacc cgggcgaggc gatccagatt gcctgcccag aacttctcgt
     1141 agaagctcag ccactcgctg gcctccctga gcgggtcggg cctaagcgaa cagatgtgct
     1201 gacggccttc aaccctgcga ttgaccatcc ccgccttctc cagaacctga acgtgtttgg
     1261 aggccgcggc aaaggacatc cgggtcggcc gggcgaggtc gccgaccgaa cattcgccgc
     1321 cggaaagctg cctcaccatc cccctccggg tctggttcga gagggcgtga aacacccggt
     1381 cgacttcctt ctgatcttca accatgtggt tgaacgtaga gcacggacgg aagattgtca
     1441 accggttggt tgaataaaag ttctcgcgga ctgcccgccc cttcgcatcg aacggacccg
     1501 gacagtgttc ggtacggtga gatcaaccga ccgatccgag ggggattgtc ttgcgcaagc
     1561 tagtgacgtt cgtggccgtt ttgctcggcc tgctcgccgt accggccaca gcttcggccc
     1621 tgaagttcga ggcggctccc ggcacgccgc tggcaccggc gacctcggtc atcgaccctt
     1681 ccccggtcat gccccctggc ttctccccga ccgacatcgc gaccggggac ttcaacaagg
     1741 acggctacgg cgacatcgcc gtgatcacgg actacatctc cgtccagggg cccgacggac
     1801 cgga
//
LOCUS       DAOQMF010000442         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47226_length_1801_cov_3.756586, whole genome shotgun sequence.
ACCESSION   DAOQMF010000442 DAOQMF010000000
VERSION     DAOQMF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47226_length_1801_cov_3.756586"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..>1801
                     /gene="typA"
                     /locus_tag="PLV77_07640"
     CDS             263..>1801
                     /gene="typA"
                     /locus_tag="PLV77_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNC15755.1"
                     /translation="MRNVAIVAHVDHGKTTLVDAMLWQSGAFRQGQDVNERVMDSMDL
                     EREKGITILAKNTAVQYGEIKLNIIDTPGHADFGGEVERGLTMVDGVVLLVDASEGPL
                     PQTRFVLGKALEAGLPVILVVNKVDRPDARIGEVVDEVYELFMDLGADGDQIEFPIVY
                     TNGKKGWASLEEGLEGEDLKPLLDLMVEHIPAPEYEEGHPLQAWVTNLDASPYLGRLA
                     LCRVMQGTIKAGSQVAWCRADGTVERAAVSELFVTEALERVSTDQAGPGEIIAVAGIP
                     EVTIGETLSDPEDPRPLPVIKVDEPSLSIVVGINTSPLAGRDGDKVTARQIQNRLDQE
                     LLGNVAIRVLPTDRPDAWEVQGRGELQLVVLLEMMRREGFELTAGQPRVLTRDVDGQM
                     HEPMERLVIDVPEEHVGAVTQLLAARKGRMENMTAQSDSWTRLDYVIPARGLVGFRTE
                     FLTETRGTGILHSIFEGWEPWAGDITTRATGSLVADRQGSTASFALFGLQERGAMFVG
                     PGEDV"
BASE COUNT          319 a          588 c          603 g          291 t
ORIGIN      
        1 ttcaaatcga agtcggagtt gggtgcgcta tgtggcgaaa tctcccttct cgattctccg
       61 gccatcaccc gccccacggc aacccaaccc atccatcaac cagttactgc agggcccggg
      121 tcggcggtgt tccccaaaaa cctccggaat gaggagcgca gcgacggttt ttgggggaca
      181 ccgccgttcc aggccctcca ccaccaagcc tttggcgtct ggctgccata gactctcccg
      241 tcctttggct cgcagggatg acatgagaaa tgtggcgatc gtggctcacg tcgaccacgg
      301 caagacgact ctggttgacg cgatgctctg gcagtccggg gccttccggc agggccagga
      361 cgtcaacgaa cgggtcatgg actcgatgga cctcgagcgc gagaagggca tcacgatcct
      421 cgccaagaac accgcggtcc agtacggcga gatcaagctg aacatcatcg atacccccgg
      481 ccacgccgac ttcggcggcg aggtcgagcg cggcctgacc atggtcgacg gggtggtgct
      541 tctggtcgac gcctccgagg ggccgctgcc ccagacgcgg ttcgtgctcg gcaaggcgct
      601 ggaggcgggc ctgccggtga tcctcgtggt caacaaggtg gaccggccgg acgcccgcat
      661 cggcgaggtc gtggacgagg tctacgagct cttcatggac ctcggcgccg acggggacca
      721 gatcgagttc ccgatcgtct atacgaacgg caagaagggc tgggcttccc tcgaggaggg
      781 cctcgaaggc gaggacctca agccgctgct cgacctgatg gttgaacaca ttccggcccc
      841 cgaatacgag gagggccatc ctcttcaggc ctgggtgacc aacctcgacg cctccccgta
      901 cctgggccgg ctcgcactat gccgggtgat gcagggcacg atcaaggccg gctcgcaggt
      961 cgcctggtgc cgggccgacg gcacggtcga gcgggccgcg gtgtccgagc tcttcgtcac
     1021 cgaagcgctt gagcgggtat caacggacca ggccgggccg ggcgagatca tcgccgtggc
     1081 cggcattccg gaagtgacga tcggcgagac cctctccgac ccggaggatc cgcggccgtt
     1141 gccggtgatc aaggtcgatg aaccttcact ttcgatcgtg gtcggaatca acacttcgcc
     1201 gctggcgggc cgggacggcg acaaggtcac cgcccgccag atccagaacc ggctcgatca
     1261 ggagctgctt ggcaatgtgg cgatcagggt gctgccgacc gaccggcccg atgcctggga
     1321 agtccaggga cggggcgagc tgcagctggt cgtactgctc gagatgatgc gacgcgaagg
     1381 tttcgagctc accgccggcc agccgcgggt gcttacccgc gatgtcgacg gccagatgca
     1441 cgagccgatg gagcgactgg tgatcgacgt cccggaagaa cacgtcggcg ccgtcaccca
     1501 gctgctggcc gcccgcaagg gccggatgga gaacatgact gcccagtccg acagctggac
     1561 ccggctcgac tatgtgatcc cggcccgcgg cctggtcggc ttccggaccg agtttctgac
     1621 cgaaacccgt ggcaccggca tcctccacag catctttgaa ggctgggagc cctgggccgg
     1681 ggacatcacg acccgggcaa ccggttcgct ggtcgccgac cgccagggtt cgaccgccag
     1741 cttcgctctc ttcgggctgc aggaacgggg ggcgatgttc gtcggaccgg gcgaagacgt
     1801 c
//
LOCUS       DAOQMF010000443         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47229_length_1801_cov_3.459336, whole genome shotgun sequence.
ACCESSION   DAOQMF010000443 DAOQMF010000000
VERSION     DAOQMF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47229_length_1801_cov_3.459336"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLV77_07645"
     CDS             complement(<1..753)
                     /locus_tag="PLV77_07645"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HNC15756.1"
                     /translation="MEIGIVGLGRMGANIARRLMRDGHRCVVYDVNPEAAKDLAGEGA
                     DPAADLAELAAKLAAPRAVWIMVPAGDITRDTIRKVAAELQPGDAIIDGGNTYYHDDI
                     HFAEELGKQGIDHLDCGTSGGVFGLERGYCLMIGGPEEAYKRLEPIFATLAPGVGEAE
                     RTPGLTGDPSPAENGYLYCGPAGAGHFVKMVHNGIEYGMMAAIAEGLNVIKGANAGVI
                     TRDGDAETAPMENPEYYQYEIDVSQVAEVWRRG"
     gene            810..>1801
                     /locus_tag="PLV77_07650"
     CDS             810..>1801
                     /locus_tag="PLV77_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNC15757.1"
                     /translation="MSKTKEREATDALVIFGITGDLARKMTFRALYRLEKRGKLDCPI
                     IGVGRNQDWHHETMKQRAEEAIRGSVDEFDLATFRRLADRMRFVNGNFDEEDLYQSLG
                     GELADYERPLFYLEIPPPLFSEVVHGLANAGLVENARVILEKPFGHDFDSAQELQADL
                     LTVLEEDQILRIDHYLGKEPVMDITYLRFANALLEPVWNRNYVSYVQMTMSENFGVED
                     RGRFYEGVGAIRDVVQNHMLQVLALLAMEAPNANQHDSIRAKKLELFEAIRSADPAKC
                     VRGQYNGYRDIPDVADDSQVETYAALELAIDNWRWSGVPFYLRTGKNLPVKHTEV"
BASE COUNT          308 a          604 c          570 g          319 t
ORIGIN      
        1 gccccggcgc cacacttcgg cgacctggga gacgtcgatt tcgtactggt agtactcggg
       61 gttctccatc ggtgcggtct cggcgtctcc gtcacgggtg atcacgccgg cgttggcgcc
      121 cttgatcacg ttcagcccct cggcgatcgc ggccatcatc ccgtactcga tcccgttgtg
      181 gaccatcttc acgaagtggc cagcgccggc cgggccgcag tagaggtagc cgttctcggc
      241 cggggaggga tctccggtga gccccggggt gcgttccgcc tcgccgaccc cgggggcgag
      301 cgtggcgaag atcggctcga ggcgcttgta ggcctcttcc ggcccgccga tcatcaggca
      361 gtagcctcgc tccaggccga aaacgccgcc ggaggtgccg cagtcaaggt ggtcgatgcc
      421 ctgtttgccc agttcttcgg cgaagtggat gtcatcgtgg tagtaggtgt tgccgccgtc
      481 gatgatcgcg tcgccgggct gaagttcggc cgcgaccttg cggatcgtgt cgcgggtgat
      541 gtctccggcc gggaccatga tccagaccgc gcgcggggcg gccagcttgg cggccagctc
      601 ggccaggtcg gcggccggat cggctccttc accggccaga tccttcgcgg cttcgggatt
      661 cacgtcatag accacgcacc tgtgtccatc gcgcatcaac ctccgggcga tgttcgcccc
      721 catccgacca agcccgacga tcccgatttc catgccgcca tattagaacc cgtggtggaa
      781 catttcgtcg gatggatacg ctgccggtca tgagcaagac caaggagagg gaagcgaccg
      841 acgcgctggt catcttcgga atcaccggtg atctcgccag gaagatgact ttccgggccc
      901 tttaccggct cgaaaagcga ggcaagctgg actgcccgat catcggcgtc ggccgcaacc
      961 aggactggca ccacgagacc atgaagcagc gggccgagga ggcgattcgc ggttcggtcg
     1021 acgagttcga tctcgcaact ttccggcgcc tcgccgatcg catgcgcttc gtcaacggca
     1081 acttcgacga ggaggatctc taccagtcgc tcggtggcga gctggccgac tacgagcggc
     1141 cgctcttcta cctcgagatc ccgccgccgc tcttctccga ggtggtccac ggtctcgcca
     1201 acgccggtct ggtcgaaaac gcccgggtca tcctcgagaa gcccttcggc cacgacttcg
     1261 actcggccca ggaacttcag gccgacctgc tgaccgtgct cgaggaagac cagatcctca
     1321 ggatcgacca ttacctcggc aaggagccgg tgatggacat cacctacctc cgcttcgcca
     1381 acgcgctgct cgaaccggtc tggaaccgca attacgtctc ctatgtccag atgaccatga
     1441 gcgagaactt cggggtcgag gaccgcggcc gcttctatga gggggtcgga gcgatccgcg
     1501 acgtggtcca gaaccacatg ctccaggtgc tggccctgct ggccatggag gccccgaacg
     1561 cgaaccagca tgactcgatc cgggccaaga agctcgagct cttcgaggcg atccgctcgg
     1621 ccgacccggc caaatgcgtc cgcggccagt acaacggcta ccgggacatc ccggacgtcg
     1681 ccgacgattc ccaggtcgag acctacgccg cgctggagtt ggcgatcgac aactggcgct
     1741 ggtcgggggt ccccttctac ctgcggaccg gcaagaacct gccggtcaag cacaccgagg
     1801 t
//
LOCUS       DAOQMF010000444         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47356_length_1799_cov_1.735092, whole genome shotgun sequence.
ACCESSION   DAOQMF010000444 DAOQMF010000000
VERSION     DAOQMF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47356_length_1799_cov_1.735092"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="PLV77_07655"
     CDS             complement(<1..1754)
                     /locus_tag="PLV77_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15758.1"
                     /translation="MPPEVHYIDPDAPPPTKDTQVEDMAMWLSPERREISLINGDFLF
                     GVGVAGQQGMGKTVLMGRMVSALLSDDRSFSGLICDPKGDLAEAVLSYLPPDRKVHYI
                     DFDDPQIGINPFEITDMADDEAIVNVILHGIIDVARTEEDESQIMASSKDFLNMALHA
                     TLATTVPFGYKPTFNHLRKWISSDPHNVAWRESLITTVLSKRRSKLWVCEGYEEYHSS
                     LKRSEAQFTTRAAAPMNKIAELLNESIAKVLRHDQAINIETAIRNNEIIIVNGRNYTQ
                     ADIIFRFLWQMVDQTLGRLETVRNKALRDREMGEDVKVPDPVKFLFGVDEAPSIMTPT
                     TAQIMARRRSAGLHMMIGWQYDEQIKNPLVREAIYSLLQNVFQFRTSVTDARQRVDLL
                     QMTYDDQQDSRLREVRTNRVSVADITNLDRFNVYIFQLVHGNRVPAYWGWTINPDGRV
                     SYAPEHLHEQRRAGGHSVRVPSPPDFEIQQADIKRRMSQKKTQPVYPDPMDNKPVNLP
                     DPGIDTNVLAQNDPDPEEGEESSESQGETSSGSSVESGSEPASSSPDSAPEVKEVPAP
                     PSTADQPSTQNGNGRVSR"
BASE COUNT          329 a          491 c          567 g          412 t
ORIGIN      
        1 ttgcggctca cccgaccgtt tccgttttgg gtggaaggct gatctgccgt gctcggcggg
       61 gcagggacct ccttgacctc gggtgccgaa tccggcgagc tggaagccgg ctcagatccg
      121 gactcgacac tcgacccact tgaggtttcg ccttgtgact cgcttgattc ttcaccctcc
      181 tcgggatcag ggtcattctg ggcaagcacg ttcgtgtcga tccccggatc cggcagattt
      241 accggcttgt tgtccatcgg atccgggtac accggttggg tttttttctg cgacattcgc
      301 cgtttaatgt ccgcctgctg aatctcgaaa tcagggggtg acgggacccg taccgaatgt
      361 ccgccggccc gccgctgctc gtgaagatgc tccggcgcat aggagacacg gccgtccggg
      421 ttgatggtcc agccccagta ggcgggaaca cgattgccgt ggaccagctg gaaaatgtag
      481 acgttgaatc gatccaggtt ggttatgtca gcaacggaaa cccggttggt gcgaacctct
      541 ctgaggcgcg agtcctgctg gtcgtcgtag gtcatttgga gaagatcgac ccgttggcgg
      601 gcgtcggtca cagaagttcg gaactgaaac acgttctgga gcagtgagta gatcgcctct
      661 cggaccagcg ggttcttgat ctgttcgtcg tactgccatc cgatcatcat gtgaagaccg
      721 gcagaacgcc gccgggccat gatctgtgcg gtggtcgggg tcatgattga cggggcctcg
      781 tcaaccccga ataggaactt gaccggatcc ggaactttca cgtcctcgcc catctcccgg
      841 tctcgaagcg ccttgtttcg aacggtttcc agccgcccca gggtttggtc gaccatctgc
      901 caaaggaacc ggaaaataat gtcggcctgc gtgtagttgc ggccgttgac aatgatgatc
      961 tcgttgtttc tgatcgcggt ttcgatgttg atcgcctggt cgtggcgaag aaccttggcg
     1021 atcgactcgt tgagcagctc ggcgatcttg ttcatcgggg ccgccgctct agtcgtgaac
     1081 tgcgcctccg agcgtttgag cgacgagtgg tattcctcgt agccctcgca aacccacagc
     1141 ttggatctgc gtttggaaag aaccgtggtg atgagcgact cacgccaggc gacgttgtgc
     1201 gggtcagacg agatccactt gcgaaggtga ttgaaagttg gcttgtagcc gaaaggcacc
     1261 gtagttgcca gagtcgcgtg aagcgccatg ttcaggaagt ccttggagga agccatgatc
     1321 tgcgattcgt cttcctcggt gcgggcaacg tcaatgattc cgtgaaggat cacgttgacg
     1381 atcgcctcgt cgtctgccat gtcggtgatc tcgaacgggt tgatgccaat ctgcggatcg
     1441 tcgaagtcga tgtagtggac tttccgatcg ggaggcagat aggagagaac cgcttctgcg
     1501 aggtcacctt tcgggtcaca gatcaggccg gagaaactcc ggtcgtcgga aagaagtgct
     1561 gaaaccatgc ggcccatcag cacggtcttc cccatgcctt gctggccggc aacgccgaca
     1621 ccgaagagga agtcgccgtt gatcagggag atctcgcggc gttcaggtga aagccacatc
     1681 gccatgtcct cgacctgggt gtctttggtc ggtggtggtg cgtccgggtc gatgtagtgg
     1741 acctccggtg gcaccgatac gagtcggcgg ttgtgccgtt tggtggtgac ccctcgaag
//
LOCUS       DAOQMF010000445         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47381_length_1798_cov_3.095238, whole genome shotgun sequence.
ACCESSION   DAOQMF010000445 DAOQMF010000000
VERSION     DAOQMF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47381_length_1798_cov_3.095238"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLV77_07660"
     CDS             complement(<1..1028)
                     /locus_tag="PLV77_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNC15759.1"
                     /translation="MSTGANIISPSDPGFAEAKFAYDLHDQKPAAICLPTTVAEVQQA
                     VRWARDEGLRIANQATGHVAVALPDLEDALLLKPQIDTEPVVDPAGGYCRVGAGTRWR
                     QVVEAAAAHGMAAMHGSSPTVGVVGYHLGGGLSFYSREHGLACNKVRAIEVVTADGEL
                     ARIDRENEPDLFWALRGGGGLFATVTAIEFDLLPVGEVYGGASFWPVETAEAALSTWL
                     DWTRTAPESVTSTFRIMNLPPFEVVPEPIRGKQLVVINGVALDKGDGEALTAQLDGLG
                     DPVMAQWGMMPGPAVASLHGDPEDPVPAIGNSTMLDGVGDELVEAFVGAASGESGCTL
                     IAAELRQLG"
     gene            1162..>1798
                     /locus_tag="PLV77_07665"
     CDS             1162..>1798
                     /locus_tag="PLV77_07665"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC15760.1"
                     /translation="MTQPAPQPEPTPGPGRNLPPWPQERGLRSWQKLALAELAAHPGG
                     SFLASATPAAGKTTFGLRVAWEMLESGRVGRVVVIAPTTHICRQWAADAARYGIDLEP
                     NRPNSDGPETSDFHGVTVTYQTVAAGPGVHADAARRPTLVIADEPHHMGDQASWGISA
                     RRAFDHATFRLLLSGTPFRSDNEAIPWVSYDEDGLSSADFTYGYPEALVDRV"
BASE COUNT          278 a          641 c          607 g          272 t
ORIGIN      
        1 ccgagctggc gcagttccgc cgcgatcagg gtgcagcccg attccccgct ggcggccccg
       61 acgaaggcct cgaccagttc gtcgccgacc ccgtcgagca tggtcgaatt gccgatcgcc
      121 ggcaccgggt cctcggggtc gccgtgaagc gaggcgacgg cggggccggg catcatgccc
      181 cactgggcca tgaccggatc accaaggccg tccagctggg cggtcagggc ctcgccgtca
      241 cccttgtcga gcgcgacgcc gttgatcacc accagttgct ttccccgaat cggttcgggc
      301 accacttcga agggaggcag attcatgatg cggaaagtgg aggtcaccga ttcgggagcg
      361 gtccgggtcc agtcgagcca ggtcgagagt gccgcctcgg ccgtttcgac cggccagaag
      421 ctcgccccgc cgtagacctc gccgaccgga agcaggtcga attcgatcgc ggtcaccgtg
      481 gcgaagagcc cgccgccgcc gcgcagcgcc cagaacaggt cgggctcgtt ttcgcggtcg
      541 atccgggcca gttccccgtc ggcggtgaca acctcgatcg cccgcacctt gttgcaggcc
      601 agtccgtgct cgcggctgta gaaactgagg ccgccgccga ggtggtaacc gaccacgcca
      661 acggtcggcg aggagccatg catggccgcc atgccgtgag cggcggcggc ttccaccacc
      721 tgccgccacc tggttccggc cccgacccgg cagtaaccgc cggccggatc gacgaccggt
      781 tcggtgtcga tctgcggttt gagcagcagg gcgtcctcga ggtcgggcag ggccacggcg
      841 acgtgaccgg tcgcctggtt ggcgatccta aggccctcgt cccgggccca gcgaacggcc
      901 tgctgtacct ccgcaaccgt cgtcggcagg cagatcgcgg caggcttctg gtcatgaagg
      961 tcgtaggcga atttcgcttc tgcgaagccg gggtccgatg gactgatgat gttcgcgccg
     1021 gtgctcattc gcccaaacta gcccaccgga cagcctcttc gcaagtgcct gattcaggtt
     1081 gaggccaagg tgaaatcggc cctcatatca cgggtggatc ggacggcgtt ccgaccgtcg
     1141 ggcagggtag gttgcgctgc cttgacccag cccgcgccac agcccgaacc aacccctgga
     1201 ccgggccgga acctcccccc gtggccgcag gagcggggcc ttcggagctg gcagaagctc
     1261 gccctggcgg agctggcggc ccatcccggc gggagcttcc tcgcctcggc caccccggcc
     1321 gccggcaaga cgaccttcgg cctgcgggtg gcctgggaga tgctcgagag cggccgggtc
     1381 ggccgggtgg tggtgatcgc cccgaccact cacatctgcc ggcagtgggc ggccgacgcg
     1441 gcgcgctacg gaatcgacct cgagccgaac cggcccaact ccgacgggcc cgagacctcc
     1501 gactttcacg gcgtgaccgt gacctaccag acggtggcgg ccgggccggg agttcacgcc
     1561 gacgcagccc gccggccaac cctggtgatc gccgacgaac cgcatcacat gggtgatcag
     1621 gccagctggg ggatcagcgc ccgccgcgcc ttcgaccatg cgacctttcg cctgctgctc
     1681 tccggtaccc cctttcgctc cgacaacgag gcgatcccct gggtcagcta cgacgaggac
     1741 ggactgagca gcgccgactt cacctacggc tacccggagg cgctggtcga ccgggtct
//
LOCUS       DAOQMF010000446         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47422_length_1797_cov_3.656142, whole genome shotgun sequence.
ACCESSION   DAOQMF010000446 DAOQMF010000000
VERSION     DAOQMF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47422_length_1797_cov_3.656142"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1078
                     /locus_tag="PLV77_07670"
     CDS             113..1078
                     /locus_tag="PLV77_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC15761.1"
                     /translation="MRGISKASGAWSRNVAIAVAAGLGLAGLFLAPSASAGLSCRYVA
                     AGPDGPRGNRLEIKATRFEEVVALLPGKGSNIEVTDDQRMKPLACKGGKPTMVNIDRV
                     NFTADRRATGSSMYIAEAPEFGPGATPAEAGGKGITFIAKGPSLSFGIGGTDGPDLIQ
                     MGMEGKAAALDFFPDLYPDSESGDNIDAKIYAKFVNLLVRGGRGADAIAGSQIDYSST
                     WFSGPLRVPTSIYGEVGPDALTGGKSMDYLDGGPGGDFMLGAQGADQLFGGGGIDTFI
                     AGPGRDEIDALDGIGGEDLRCGRGRDLARMDLEDQDRDCESFRFP"
     gene            1056..>1797
                     /locus_tag="PLV77_07675"
     CDS             1056..>1797
                     /locus_tag="PLV77_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNC15762.1"
                     /translation="MSPSVSPENANRGQTRPVTFLTDFGYSDEYPGVCRLVIASIDPR
                     IKVIDLTHGIPPGDIRRGGLALAAAVRFSAPAVHLAVVDPGVGSRRRAVVLTCGEHHL
                     VGPDNGLLSLAAAVLGGVEAAFDVSATPVRVGGSDTFHGRDVFSPVAAELARGAAPGE
                     LGEAVGFRELAPLELPEPLVTPEQVDAHVLYPDQYGNVILNIHSEVLDGTFFTEGDAI
                     SLETSLQKLEVPLRRTFADVEPGEPVLFT"
BASE COUNT          288 a          611 c          619 g          279 t
ORIGIN      
        1 gacggcagcg gcctgttcgc cgtccccccg ggggtcagtg aaccggacgg cttcatcggc
       61 gaggggctct tcagctagcc cgaccgcgcg gccgggcaaa tagaatccgg ccatgcgggg
      121 aatcagcaag gcaagcgggg cgtggagccg gaatgtcgcg atcgcggttg ccgccgggct
      181 cgggttggcc gggctgtttc tggcaccttc cgcctcggcc ggactcagct gtcgctacgt
      241 ggcggcagga ccggacggcc cgaggggcaa ccggctggag atcaaggcga ccaggttcga
      301 ggaagtggtc gcgctgctgc ccggcaaagg ctcgaacatc gaagtcaccg atgaccagcg
      361 catgaagccg cttgcctgca agggcggcaa gccaaccatg gtcaacatcg accgggtcaa
      421 cttcaccgcg gaccgcaggg cgaccggctc gtcgatgtac atcgccgaag cgccggagtt
      481 cggtcccggg gccaccccgg ccgaggccgg gggcaagggc atcaccttca tcgccaaagg
      541 tccgtcgctc agcttcggga tcggcggcac cgacggcccg gatctgatcc agatgggcat
      601 ggaaggcaag gccgccgcgc tcgacttctt tcccgatctc tatcccgaca gtgaatcggg
      661 cgacaacatc gacgcgaaga tctacgcgaa gttcgtcaac ctcctggttc gcggcgggcg
      721 gggagccgac gcgatcgccg gcagccagat cgattactcg agcacctggt tcagcggtcc
      781 gctgagagtt ccgacctcga tctacggcga ggttggcccc gacgccctga ccggcggcaa
      841 gagcatggat tacctggacg gcggccccgg cggtgacttc atgctgggcg cgcagggagc
      901 cgaccagctc ttcggaggcg gcggcatcga caccttcatc gccggcccgg gccgggacga
      961 gatcgacgcg ctcgacggaa tcggtggaga ggatctgcgt tgcggccgcg gcagggatct
     1021 ggcccggatg gacctggaag atcaggaccg ggattgtgag tccttccgtt tcccctgaga
     1081 acgcaaatcg ggggcaaacg cggccagtca ccttcctgac cgacttcggg tacagcgacg
     1141 agtaccccgg ggtctgccgg ctggtgatcg cctcgatcga tccccggatc aaggtcatcg
     1201 acctgaccca cggcattccc cccggtgaca tccggcgcgg cggcctggcc ctggctgcgg
     1261 cggtacggtt ctccgccccg gcggttcacc tggccgtggt cgatcccgga gtcggcagcc
     1321 gccgccgggc cgtggtgctc acctgcggcg agcatcatct ggtcggtccg gacaacggcc
     1381 tgctctccct ggccgccgcg gtgctgggcg gggtcgaggc cgccttcgac gtctccgcca
     1441 ccccggtccg ggtcggcggg agtgacacct tccacggccg tgacgtcttc agcccggtgg
     1501 cggccgaact ggcccgcggt gcggcccccg gcgaactcgg ggaagccgtc ggtttccggg
     1561 agctggcccc gctcgagctg cccgaaccgc tagtcacccc ggagcaggtg gacgcccatg
     1621 tgctctaccc ggatcagtac ggaaacgtga tcctgaacat ccattccgag gtgctggacg
     1681 ggaccttctt caccgagggt gacgcaatct cgctggagac gagcctccag aagctcgagg
     1741 tgccgctccg ccgcaccttc gccgatgtcg agcccggcga accggtcctc ttcaccg
//
LOCUS       DAOQMF010000447         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47524_length_1795_cov_2.902299, whole genome shotgun sequence.
ACCESSION   DAOQMF010000447 DAOQMF010000000
VERSION     DAOQMF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47524_length_1795_cov_2.902299"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..639)
                     /locus_tag="PLV77_07680"
     CDS             complement(58..639)
                     /locus_tag="PLV77_07680"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNC15763.1"
                     /translation="MVKIYTKKGDDGTTGLWYGGRVEKSGLRTDAYGTLDEACSALGV
                     ARAMCDDSQEELAADILALQNELFVAGAELATAPEAAGRLEDGISRITAGMVDALDPE
                     IDKYMAHVNLPPKFVIPGGNRLSAQLDVARTIVRRAERAVVRIQLADELASTEILRFL
                     NRASDLVFSMARYADVDFPDLFEGRRKSGEDEE"
     gene            complement(664..1383)
                     /locus_tag="PLV77_07685"
     CDS             complement(664..1383)
                     /locus_tag="PLV77_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15764.1"
                     /translation="MILGSLSYSFTNEESFDAIRYLTLTNLGPGASQPISIWSLGMHG
                     GQYADLMAIGESHGGLNGCFEGQSLAVGAQCSIHVSWAPRNRSTTDMTGVVEIRLWNT
                     TTVLASVPVSAAYQSRAYITGGLSPASLTANQGFKQRGDATLTNVGDSPATPSVSVSG
                     PDAQFFAVDSNRENTCEGFSIAVDATCKLPIVFCAGVVRDYEASLVVTVNNVVTESSP
                     LRATVRQLNDHPQCTHLQTND"
BASE COUNT          308 a          556 c          608 g          323 t
ORIGIN      
        1 ccgccactgg ccccggtcac cacgcagacc cggtccctca gtcccaggtc caccttgcta
       61 ctcctcgtct tcgcccgact tgcggcggcc ctcgaagagg tccgggaagt cgacgtcggc
      121 ataccgcgcc atcgagaaga caaggtccga ggcccggttc aggaagcgga gaatttcggt
      181 cgaggcaagc tcgtcggcaa gctggatccg gaccacggcc cgctcggccc ggcgcacgat
      241 ggtgcgggcg acgtcgagct gggctgaaag ccggttaccg ccgggaatca caaacttggg
      301 cggcagattc acgtgggcca tgtacttgtc gatctcggga tcgagcgcat cgaccatccc
      361 ggcggtgatc cggcttattc cgtcttcgag acgcccggcc gcctccggtg cggtcgccag
      421 ctccgcgccg gccacgaaga gctcgttctg aagggcgagg atgtctgccg ccagttcctc
      481 ctggctgtcg tcgcacatcg cccgcgccac gcccaatgca gagcaggcct cgtcaagcgt
      541 tccgtaggcg tcggtcctca gccccgactt ctcgacccgg cccccgtacc agagtccggt
      601 cgtcccgtca tcgcccttct tcgtgtagat cttgaccaca ccctgatcct agaggagcag
      661 gggtcagtcg ttcgtttgaa ggtgggtgca ttgggggtgg tcgttgagct gcctgaccgt
      721 cgcccgcagc ggtgacgatt cggtgaccac gttgttgacc gtgacgacaa gagacgcctc
      781 gtagtcacgc acgacgccag cacaaaagac gatcgggagt ttgcaggtgg cgtcgacggc
      841 gatcgagaac ccttcacagg tgttctcgcg gttcgagtcg accgcgaaga actgggcatc
      901 gggaccggag acgcttacgc tcggtgtcgc aggggaatct ccgacgttcg tgagggttgc
      961 atcacctcgc tgcttgaacc cctggttggc ggtaagcgag gcgggagaga gaccgcctgt
     1021 gatgtaggca cggctctggt aggcggcgga gaccgggacc gaagccagga cggtggtcgt
     1081 gttccatagc cggatctcga cgacccccgt catgtcggtg gtgctgcggt tgcggggagc
     1141 ccaggaaacg tggatcgagc actgggctcc caccgcgagc gattgaccct cgaagcagcc
     1201 gttcaaacct ccgtgggatt caccgatggc catcaggtcg gcgtactggc cgccatgcat
     1261 gcccaatgac cagatcgaga tcggctggga agcgcctggt cccaggttgg tcagggtcag
     1321 gtacctgatc gcatcgaagg attcttcgtt ggtgaacgag tagctgagcg agcccagaat
     1381 caaggggctg ggggagaggg ccaagccggt tgtcggaccg gtgggtccgg ttggtcccgt
     1441 ggggcccgtg ggagaggtcg gtccggtggg gccagtcgga gaggtcggtc cggtcggacc
     1501 cgtcgggccg gtaggcccgg tccctcccgg catcacctgg atcgcgttcg cgagggtgcg
     1561 acagttgttg ctctctttcg attccctgat catgcggtcg ccatccgcgc aaagaatcac
     1621 gcgccgggaa ccggcgggct ggccggaggg gatggtcata gacacctcct ccagaaggag
     1681 tcgcccaccc ccaggttcgc gaagggtgac ggtatcgaat tcgatctggc gagcgagtgg
     1741 attgaggaat tccgcgccaa caacctgtcc cacctcgccc cggcgttcga ggaat
//
LOCUS       DAOQMF010000448         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47864_length_1788_cov_3.568379, whole genome shotgun sequence.
ACCESSION   DAOQMF010000448 DAOQMF010000000
VERSION     DAOQMF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47864_length_1788_cov_3.568379"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..761)
                     /locus_tag="PLV77_07690"
     CDS             complement(72..761)
                     /locus_tag="PLV77_07690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC15765.1"
                     /translation="MARATSVFEKQAATYRGPREKLIPQMERFYGMVPEAIGLAEPET
                     GREGQPLRILDLGAGTGMLSAVVAEAWPDAAFTLFDFSPRMLEQAKESLGEAVRVLTG
                     DMYEGIPAGPFDAVISALAIHHLTDEGKRSVYEAVFGELRPGGIFVNAEHVLGETPAL
                     QQHYAKWHRRQAFEKGLTESEWADTVERMSHDHLTPLSTQLGWLEEIGFTDVDCLFKD
                     HGFAVLFGRRP"
     gene            complement(789..1388)
                     /locus_tag="PLV77_07695"
     CDS             complement(789..1388)
                     /locus_tag="PLV77_07695"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNC15766.1"
                     /translation="MIEQLAVEVGDRRVLDAIAAVPRDLFVPEDQRRFAWENRPLPIG
                     RGQTISQPLVVATMCELLELSEDDLVLDVGGGSGYHAAVLARLARRVISIERQPELVE
                     AARRSLAAAGVVNVEMICGDGSVGCPGEAPFDAINVAAAVRGEVPEALPGQLAEGGRM
                     VVPVGRRHQWLELWRRREGRLSRERMVAVAFVPLVEEGD"
     gene            complement(1399..>1788)
                     /locus_tag="PLV77_07700"
     CDS             complement(1399..>1788)
                     /locus_tag="PLV77_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC15767.1"
                     /translation="PLTATRRKYQTVTRNGVKIPIHGGDYEPYGSFNTIWGPWIPGKG
                     ITEVSDGSSFIQAVRLDGSKCPRASMILTYSQSENPKSKHYADQTKLFSKKGWSLDRF
                     CPNQQKKSPGLKVKRFGGGAKAESRGY"
BASE COUNT          278 a          634 c          566 g          310 t
ORIGIN      
        1 ggaggtgccg gccgaggtcg ccgagctgat cgccgggacc gcccgcctgg cctgagccgc
       61 gcgtcggtcg ttcagggccg gcgaccgaag agcacggcga agccgtggtc cttgaagaga
      121 cagtcgacgt cggtgaaacc gatctcttcg agccatccaa gctgggtcga aagcggggtc
      181 aggtggtcgt ggctcatgcg ttcgacggtg tcggcccatt cggattcggt caggcccttc
      241 tcgaaggcct ggcggcggtg ccattttgcg tagtgctgct gcagggccgg ggtctcgccg
      301 aggacgtgct cggcgttgac gaagatgccg cccggtctca actccccgaa aaccgcctcg
      361 tagaccgacc gctttccctc gtcggtcagg tggtgaatgg cgagggccga gatcaccgcg
      421 tcaaagggcc cggccgggat tccctcgtac atgtccccgg tcagcacccg gaccgcttct
      481 cccaggcttt ccttcgcctg ttccagcatc cgcggggaaa agtcaaagag cgtgaacgcc
      541 gcatccggcc aggcttccgc gaccaccgcg gagagcattc cggttcccgc gccgaggtcg
      601 aggatcctga gcggctggcc ctccctcccg gtttcgggtt cggccagtcc gatcgcttcc
      661 ggcaccatcc cgtagaagcg ctccatctgg ggaatcagct tctcccttgg gccgcgatag
      721 gtcgcggcct gcttctcgaa aaccgaggtc gccctggcca tgggtctgat cctagatcct
      781 cgaccgggtc agtccccttc ttccaccagg gggacgaagg cgacggcaac catgcgctcg
      841 cgggagagcc ggccttcccg ccttcgccag agctccagcc actgatgtcg gcgcccgacc
      901 ggcaccacca tccgcccgcc ctcggcgagt tgccccggga gcgcctccgg cacctcgccc
      961 cgaactgccg cggccacgtt gatcgcgtca aaaggcgctt cccccgggca gccgaccgac
     1021 ccgtcaccgc agatcatctc cacgttcacg acgccggccg cagccaggga ccggcgagcc
     1081 gcttcgacga gttccggctg gcgctcgatt gagatcaccc ggcgggcgag cctcgccagc
     1141 accgctgcgt gatagcccga gccaccgccc acgtcgagga cgaggtcgtc ctcactcagc
     1201 tcgagcagct cgcacatcgt cgccacgacc agtggctggg agatcgtctg gccccggccg
     1261 atcggcaacg ggcggttctc ccaggcaaac cggcgctggt cctccggaac gaagagatcg
     1321 cggggaacag cggcgatcgc gtcgaggacc ctgcggtccc cgacctcgac cgccagctgt
     1381 tcgatcagcc gatcccgctc agtacccgcg actttctgcc ttcgctcccc cgccgaatcg
     1441 cttcaccttg agccccggtg acttcttctg ctggttgggg cagaagcgat ccagcgacca
     1501 gcccttcttc gagaagagct ttgtctggtc ggcgtaatgc ttcgacttcg ggttctccga
     1561 ctgcgagtag gtcaggatca tgctcgcccg cgggcacttc gaaccgtcca gccgaaccgc
     1621 ctggatgaag ctggagccgt cgctgacctc ggtgatgccc ttgcccggaa tccaggggcc
     1681 ccagatcgtg ttgaaggaac cgtagggctc gtaatcccca ccgtggatcg ggatcttcac
     1741 cccgttgcgg gtcaccgtct ggtacttgcg gcgggtcgcg gtcagcgg
//
LOCUS       DAOQMF010000449         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47910_length_1787_cov_4.516166, whole genome shotgun sequence.
ACCESSION   DAOQMF010000449 DAOQMF010000000
VERSION     DAOQMF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47910_length_1787_cov_4.516166"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLV77_07705"
     CDS             complement(<1..205)
                     /locus_tag="PLV77_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HNC15768.1"
                     /translation="MPPVAPSEYPPPFDSESVITAPTDPEDERIEVGVAIVGGGPAGL
                     AAAVKTLQLLEDKPELMEKLGEVP"
     gene            complement(314..1288)
                     /locus_tag="PLV77_07710"
     CDS             complement(314..1288)
                     /locus_tag="PLV77_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNC15769.1"
                     /translation="MAGILVYALHDDEGNFNKNSLGALSEAAKLAGELGTEAAAVIVG
                     DISDDGAAGLGNYGVTKVYRAKDVPEGLAQPIVDVMAKVIQDNGYTYALFGGGLLGFE
                     IGAGLTARLGAGVTMEVTKVKVDGGKLVSERPILQDSKIADVGYVSEPGIIIGRLNAF
                     DVNESGGTAEVVDVDVELSEFSTKAEMITRGEQRGADVDIEAADILIGGGRGLGSADG
                     FKDCEELAGAFSNGAVAATRAVVDAGWYPYAAQIGQTGKTVAPKLYLAVGVSGAIQHK
                     VGMQNSENIVAINKDANAPIFEFSDLGIVGDLNKIMPKLTEAIKAKKG"
     gene            complement(1289..>1787)
                     /locus_tag="PLV77_07715"
     CDS             complement(1289..>1787)
                     /locus_tag="PLV77_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNC15770.1"
                     /translation="ATGLALAKVLESEGPDLVLLGQQSDDGECYTIGAVVADHLKMPS
                     LTQVIDMKVDGDKIRCERQAEYGYDTVALTLPAVISVGDAINEPRYPSLKAIMGAKKK
                     PLDSKSAADVGIDAGQVGEAGSQTTVISLNEPPQKEAGEIIEDEDTAETVEKIVAWLE
                     ERKLI"
BASE COUNT          272 a          558 c          605 g          352 t
ORIGIN      
        1 caggaacctc gccgagcttc tccatcagct cgggcttgtc ctcgagcagc tggagggtct
       61 tgacggcagc ggcgagaccg gccggtccac cgccgacgat cgctacgccg acttcaatgc
      121 gctcatcctc gggatcggtg ggcgcggtga tgacgctttc ggaatcaaag gggggcggat
      181 attcggaggg cgctacgggc ggcatgctct tggtcctttc gggacgaatc ggtggtgaga
      241 cgagaagttc ttcaatcggg gtgaaactac gggccgggga agctttgacg ctcccccggc
      301 cggatactgc ttgctagccc ttcttggcct tgatggcctc ggtgagcttc ggcatgatct
      361 tgttcagatc gccgacgatg ccgaggtcgg agaactcgaa gatcggggcg ttcgcgtcct
      421 tgttgatcgc cacgatgttt tcggagttct gcatgccgac cttgtgctgg atggcgccgg
      481 agacgccaac cgccaggtac agcttcggcg cgaccgtctt gccggtctgg ccgatctggg
      541 cggcgtaggg gtaccagccg gcgtcgacca ccgcgcgggt tgcggcaacc gcaccgttgc
      601 tgaaggcacc ggcgagttcc tcgcagtcct tgaagccgtc ggccgagccg aggccacgac
      661 cgccaccgat caggatgtcg gcggcttcga tgtccacgtc ggcgccacgc tgctcgccgc
      721 gggtgatcat ctcggccttg gtcgagaact cggagagctc gacgtcgacg tcgacgacct
      781 cggcggttcc gccgctttcg ttcacgtcga aggcgttgag gcggccgatg atgatgccgg
      841 gctcggagac gtagccgacg tcggcgatct tcgagtcctg caggatcgga cgctcggaga
      901 cgagcttgcc accgtcaacc ttgaccttgg tgacttccat ggtcacgccg gcgccgaggc
      961 gggcggtcag gccggcaccg atctcgaagc cgagcaggcc gccgccgaac agggcgtagg
     1021 tgtaaccgtt gtcctggatg accttggcca tcacgtcgac gatcggctgg gcgaggccct
     1081 ccggaacgtc cttcgcgcgg tagaccttgg tcacgccgta gttgccgaga ccggcagctc
     1141 cgtcgtcgga aatgtcgccg acgattacgg ctgcggcttc ggtgccgagc tcgccggcca
     1201 gcttggcggc ctcggagagg gcgccgagcg agttcttgtt gaagttgccc tcgtcatcgt
     1261 gaagggcgta aacgagaatg ccggccattt agatcagctt cctttcctcg agccacgcaa
     1321 cgatcttctc gaccgtttcc gcggtgtctt cgtcttcgat gatctcgccg gcttccttct
     1381 ggggcggctc gttcagcgaa atgacggtcg tctgtgagcc ggcttcgccg acctggccgg
     1441 cgtcgatgcc gacgtcagcg gcggacttgc tgtccagcgg cttcttcttg gcgcccatga
     1501 tggccttgag gctcgggtaa cgcggctcgt tgatcgcgtc accaaccgag atcacggccg
     1561 gcagggtcag ggccacggtg tcgtagccgt actcggcctg acgctcgcag cggatcttgt
     1621 cgccgtcgac cttcatgtcg atgacctggg tcagcgacgg catcttcagg tgatcggcca
     1681 caacggcacc gatcgtgtag cactcgccgt catcggactg ctggccgagc aggaccaggt
     1741 ccgggccctc ggactcgagg accttggcca gggcgaggcc ggtggcg
//
LOCUS       DAOQMF010000450         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_47928_length_1787_cov_2.777714, whole genome shotgun sequence.
ACCESSION   DAOQMF010000450 DAOQMF010000000
VERSION     DAOQMF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47928_length_1787_cov_2.777714"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLV77_07720"
     CDS             complement(<1..689)
                     /locus_tag="PLV77_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNC15771.1"
                     /translation="MASRREKKRTERTRAQMPRWMFETRTHQWHEAGLGEQIVGPPQG
                     RTWPRLILFAVLIAAVLVAFNHRQDIAPGYGTEARILTAILLFGLGWGFASALGRTVT
                     PFIMRRLDPGTAGTLGFVIRLGTILIVGLVALGIAGVKPQTLAVGGAATAIVLGLAAQ
                     QTLGNVFAGVVLQSTRPFRVGERVRLTGGPMAGSVEGTVSSLGLFYTTILKGQDRMMI
                     PNNLLLLLAVEP"
     gene            complement(772..>1787)
                     /locus_tag="PLV77_07725"
     CDS             complement(772..>1787)
                     /locus_tag="PLV77_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15772.1"
                     /translation="LGVENNVLVCGSDWVGMADQDIGSAINALLDLSKFPALPDRLQQ
                     GFLNFLFLGRLMIHPDGFAADPAFKVDGHSVIDTSDLFYYGNSQGGIAGGALTAVATD
                     FTRSVLYVPGMNYSMLLTRSTDFEDYKSILYPAYQNELERPSIFALMQSMWDRGEPNG
                     YANNMTSNPLPNTPAHHVMIEMSYGDHQVANVATEVEARTIGAPLRTPTIDADRAATG
                     LIEPFYGHDTLGDLAGPAADGNAYFVWDIGPQRLQEDPEGSGNMKLFGTDPAPLTNTP
                     PVTGESDTPLGETSGIDPHDTVIEESPLIRKQIADFLKTDGKVTNPCGLNPCYAAKWK
                     GMP"
BASE COUNT          294 a          560 c          621 g          312 t
ORIGIN      
        1 ggctcgaccg ccagcagcag gagcaggttg ttgggaatca tcatccggtc ctggcccttg
       61 aggatcgtcg tgtagaagag gccgagcgaa ctgaccgttc cctcgaccga gccggccatc
      121 ggcccgccgg tcaggcgcac ccgctcgccg acccggaacg gccgggtgct ctggagcacg
      181 accccggcaa agacgttgcc aagggtctgc tgggcggcga gaccgaggac gatcgcggtg
      241 gccgcaccgc cgaccgccag ggtttgcggt ttgaccccgg caatcccgag cgcgaccaga
      301 ccgacgatca ggatcgtgcc cagccggatg acgaagccga gtgtgccggc cgttcccggg
      361 tcgagccggc gcatgatgaa gggagtgacc gtgcggccga gggcggaggc gaaaccccag
      421 cccaggccaa agagcaggat cgcagtgagg atcctcgcct cggtgccgta gcccggggcg
      481 atgtcctgcc ggtgattgaa ggcgaccagg accgccgcga tcagcacggc gaagaggatc
      541 agcctcggcc aggtccgtcc ctgtggcggg ccgacgatct gttcgccaag gccggcttca
      601 tgccactggt gggtccgggt ctcgaacatc cacctcggca tctgggcccg ggtgcgttcg
      661 gtccttttct tctcacgtcg tgaagccacg acggttcacc atacaaagag cccgggttga
      721 agctgcccgc agatcaggcg aacggctcca acccgggcgg gaacttccgg cctacggcat
      781 gcccttccac ttggcggcgt agcaggggtt gagaccacag gggtttgtga ccttgccgtc
      841 cgtcttgagg aagtcggcga tctgcttgcg gatcagcggt gactcttcga tcaccgtgtc
      901 atgcgggtcg atgccgctgg tctcgccgag cggggtgtcg gactcgccgg tgaccggcgg
      961 ggtattggtc agcggtgccg gatcggttcc gaacagcttc atgttgccgc tgccttccgg
     1021 atcttcctgc agccgctggg ggccgatgtc ccagacgaaa taggcgttgc cgtcggcagc
     1081 cgggccggcc aggtcaccca gggtgtcgtg accgtagaac ggctcgatca ggcccgtggc
     1141 ggcccggtcc gcgtcaatcg tcggcgtcct cagcggagcg ccgatcgtcc gggcctcgac
     1201 ctcggttgcg acgttcgcca cctgatggtc gccgtaggac atctcgatca tcacgtggtg
     1261 ggccggggtg ttcggcaacg gattgctggt catgttgttg gcgtagccat ttggctcgcc
     1321 gcggtcccac atcgactgca tcagggcgaa gatcgagggt cgctcgagct cgttctggta
     1381 ggccgggtag aggatcgact tgtagtcctc gaagtcggtg ctgcgagtca gcagcatcga
     1441 gtagttcata cccggcacgt agagcaccga cctggtgaaa tcggtcgcca ccgcggtcag
     1501 ggcaccgccg gcgattccgc cctggctgtt gccgtagtag aagaggtcac tggtgtcgat
     1561 caccgagtgt ccgtcgacct tgaaggccgg gtcggccgcg aagccgtccg ggtggatcat
     1621 cagccggccc aggaacagga agttgaggaa accctgctgc aggcgatccg gcagggccgg
     1681 gaacttgctc aggtcgagca gcgcgttgat cgcggatcca atgtcctggt cggccatccc
     1741 gacccagtcg gagccgcaga ccaggacgtt gttctcgacg ccgagct
//
LOCUS       DAOQMF010000451         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_48070_length_1784_cov_4.873337, whole genome shotgun sequence.
ACCESSION   DAOQMF010000451 DAOQMF010000000
VERSION     DAOQMF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48070_length_1784_cov_4.873337"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..>1784
                     /locus_tag="PLV77_07730"
     CDS             156..>1784
                     /locus_tag="PLV77_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNC15773.1"
                     /translation="MNFAACRPSRASLPVFACLLIAAFVLPAFLPAPAEARFGQWLNF
                     GSSYGVPSGRIGIQFTRDVGLDESKATGNRPGGRVGFLSGGVWYHATRLADTVALPDP
                     SILNTNDPAGRQIRIEVRDIGNETMRLSAEVLGSGPEPVDSIGIGFGLKDSERLLGFG
                     ERSDHVNQRGNEVESYVGEGPYQETDYPIIKNTVPSWGVRERSDATYFPMPWFISTRG
                     YGVLAGNAETSRFRLGTESKGEWSFEADARRMTLRFFAGPRPAGVLRRMTRAIGRQPK
                     PRAPWIFGPWFQTGHQNTSPNELDYVETLRDADAPVSAVETHMRYMPCGSDLGQEAAE
                     KARVAGLHAGGLAAITYTREAICASYSGPYDQAFSQGAFIRHQDGTPYTFNSFVGSGV
                     TQLGMLDFTNPAAQPIYAGILDRAYTAGYDGWMEDYGEYAPPDSVSFDGIPGKRLHNL
                     HPVFYHRGGLRYSNSKKRPIVSFVRSGFTGSARYSQIVWGGDPTTGWGFDGLQSQVTE
                     ALTMGLSGVSNWGSDIGGFFSFSPQKLDAELLARW"
BASE COUNT          295 a          644 c          546 g          299 t
ORIGIN      
        1 ggccaccgca tcgagctgct cctgggttgc cccgtcgtca ccaccgcatc cgtatgcaaa
       61 gaggccgaga acgagcaagg cgaggacggc ggatactccc ttgagactgg acatgaccga
      121 agcttagccc tggtcgtagt gttctggaag aactcatgaa ttttgctgcc tgccgtccgt
      181 cacgcgcctc cctgcccgta ttcgcctgcc tgctgatcgc cgctttcgtc ctgcccgcct
      241 tcctgcccgc gccggccgaa gccaggttcg gccagtggct caatttcggc tcctcctacg
      301 gggtgccgag cggcaggatc gggatccagt tcacccgtga cgtcggcctc gatgaatcga
      361 aggcgaccgg caatcgcccg ggcggccggg ttggctttct ttcgggcggt gtctggtacc
      421 acgccacgcg gctggccgat accgtcgccc tgcccgaccc gtcgatcctg aataccaacg
      481 acccggccgg ccgccagatc aggatcgaag tgcgcgacat cggcaacgag acgatgcggc
      541 tgagcgccga agttctcggc agcggcccgg aaccggtcga ctcgatcggg atcgggttcg
      601 gcctgaagga cagcgagcgg ctgctcgggt tcggcgagcg ctccgaccac gtgaaccagc
      661 gcggcaacga ggtcgaaagc tacgtgggcg agggccccta ccaggaaacc gattacccga
      721 tcatcaagaa cacggtcccg tcctggggag tgcgtgagcg ctccgacgcg acctacttcc
      781 cgatgccctg gttcatctcc acccgcggtt acggcgtgct cgccggcaat gccgaaacca
      841 gccgcttccg gctcggaacc gagagcaagg gcgaatggtc cttcgaggcc gatgcccgcc
      901 ggatgaccct gcgcttcttt gccggacccc gaccggccgg ggtgctgcgg cgcatgacca
      961 gggcgatcgg ccgccagcct aagccgaggg caccctggat cttcggcccc tggttccaga
     1021 ccggccacca gaacacttca cccaacgaac tcgattacgt cgagaccctg cgcgacgccg
     1081 atgccccggt ctccgcggtc gagacacaca tgcgctacat gccctgcggt tccgacctcg
     1141 gtcaggaagc ggccgagaag gcccgtgtcg ccggactgca tgccggtggc ctggccgcga
     1201 tcacctatac ccgcgaggcg atctgcgcct cctactccgg gccgtacgat caggccttct
     1261 cgcagggcgc cttcatccgc caccaggacg gcacccccta caccttcaat tccttcgtcg
     1321 gcagcggggt gacccagctc ggcatgctcg acttcaccaa tccggccgcc cagccgattt
     1381 acgccggcat cctcgatcgc gcctacaccg ccggctacga cggctggatg gaggattacg
     1441 gcgagtacgc cccgccggac tcggtttcct tcgacgggat ccccggcaag cgcctgcaca
     1501 acctccaccc ggtcttctac caccggggcg gcctgcgcta ctcgaactcg aagaagcggc
     1561 cgatcgtgag cttcgtgcgt tccggcttca ccggctcggc ccgctactcg cagatcgtct
     1621 ggggcggcga cccgaccaca ggctggggct tcgacgggct ccagtcgcag gtcacggaag
     1681 ccctgacaat gggcctttcg ggcgtctcca actggggctc cgacatcggc ggcttcttca
     1741 gcttctcgcc gcagaaactc gacgccgaac tgctcgcccg ctgg
//
LOCUS       DAOQMF010000452         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_48294_length_1780_cov_4.862029, whole genome shotgun sequence.
ACCESSION   DAOQMF010000452 DAOQMF010000000
VERSION     DAOQMF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48294_length_1780_cov_4.862029"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1780
                     /locus_tag="PLV77_07735"
     CDS             <1..>1780
                     /locus_tag="PLV77_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15774.1"
                     /translation="DIDGDGAPEVVTTAGIRVYAWESSGEEVTGFPVTSDLGKCGAAL
                     ESQPLSHPKCGFIASPAIARLQGPDAAPDIVVPGLDGYLYAFSGQGDAVPGYPVRLVD
                     PDIPAGEQMLAESINSPAVGDLNGDGRDDAVVATNETYDAQPPDFESISGGVVGSALT
                     DILANAAGGSERVYAVDSRGNGFEGNGGSPYLDGWPIKLNGAIQSTLPLIGPGQDPAI
                     TMVGGEPRVVASATGSATIGIYKPDGTVALNVEQAVPGAASVADGAIGTGAVNLFESA
                     SLGKLTPGATNPSIVKYGLGLPGILNLLLVGQNLPYNHLIGAYDTATGLSAPSFPRVT
                     EDFQFLSSSTIANLNGSQLSNQVLAGTGLGLINAYDGLTGLNVDGFPKVTGGWLFAPP
                     TVSDDGRMAAITREGYLFEWSLPDIPACQTEWPSFRHDPQQSGNYDRDGSSPGGLGDV
                     AEEGGTLGFTATGDDGECGVADHYEVVTSDSPVTGESFAAAQPVTGAPAPAAAGTGQS
                     FVLPETARYVAIRAVDEAGNVGPMSVVDRGDTGPTGPTGPTDPTGPTGPTDPTGPTGP
                     TDPTGPTGPTDPTGPTGPTSPTGPTGP"
BASE COUNT          294 a          639 c          584 g          263 t
ORIGIN      
        1 agacatcgat ggcgacggcg cgccggaagt ggtgacgacg gccggaatca gggtctacgc
       61 ctgggaatcc agcggtgaag aagtcaccgg gttcccggtc acgtcggacc tcggcaagtg
      121 cggtgccgcg ctcgaaagcc aaccgctttc gcatcccaag tgcggattca tcgccagccc
      181 cgcgatcgcg cgacttcagg gtccggacgc ggcgcccgac atcgtcgtcc ccgggctcga
      241 cggttacctc tacgcgttta gcggccaggg cgacgccgtt ccggggtacc cggtgcgtct
      301 ggtcgacccc gacatcccgg cgggcgagca gatgctggcc gaatcgatca actcgcccgc
      361 ggtcggtgac ctgaacggcg acggtcgcga cgatgccgtg gtcgcaacca acgagaccta
      421 tgacgcccag ccgccggatt tcgagagcat ctccggcggc gtggtcgggt cggccctcac
      481 cgacatcctc gccaacgcgg cgggaggttc cgagcgggtc tacgccgttg actcacgcgg
      541 caacggtttt gaaggcaacg gcggaagccc ctatctggac ggctggccga tcaagctgaa
      601 cggcgcgatc cagtccaccc tgcccctgat cgggccggga caggatccgg cgatcacgat
      661 ggtgggcggt gaaccgcggg tcgtcgcgtc ggccacgggt tcggccacga tcggcatcta
      721 caagccggac ggcaccgtgg ccctgaacgt cgagcaggcg gtcccgggag cggcttcggt
      781 ggccgacggg gcgatcggaa cgggtgcggt caatctcttc gaatcggctt cgctcgggaa
      841 gctcacgccg ggcgccacga atccgagcat cgtcaagtac ggtctcggcc tgcccggcat
      901 cctgaaccta ctgctggtcg gccagaacct gccctacaac catctgatcg gggcctacga
      961 caccgcgacc ggcctttcgg ctccgtcctt cccccgggtt accgaggact tccagttcct
     1021 ttcctcctcg acgatcgcca acctgaacgg cagccagctt tccaaccagg tgctggccgg
     1081 gaccgggctc ggcctgatca acgcctacga cggactgacc ggcctcaatg tcgatggctt
     1141 cccgaaggtg accggcggct ggctctttgc tcctccgacc gtctccgacg acggcaggat
     1201 ggccgccatc acccgcgagg gctatctctt cgagtggtcc ctgccggaca tcccggcctg
     1261 ccagaccgag tggccatcct tccgtcacga cccgcagcag agtggaaact acgaccgtga
     1321 cggctcaagc cccggcgggc tcggtgacgt cgccgaggaa ggcggaacgc tcggcttcac
     1381 cgcgaccgga gacgacggcg agtgcggggt cgccgatcac tacgaggtgg tcacctccga
     1441 ctctccggtc accggtgaat ccttcgcggc ggcccaaccg gtcaccggag ctccggcccc
     1501 ggcggcggcc gggaccgggc agtcattcgt cctgcccgag accgccaggt acgtggcgat
     1561 ccgggcggtc gatgaagccg gcaacgtcgg tccgatgtcg gtggtcgacc gcggtgatac
     1621 cgggccgacc ggaccaaccg gtcccaccga cccgaccgga ccgactggcc cgaccgatcc
     1681 cacgggaccc accggaccga ccgatccgac cggtccgacg ggcccaaccg acccgaccgg
     1741 tccaaccggg ccgaccagtc ccaccggtcc gacgggcccg
//
LOCUS       DAOQMF010000453         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_48319_length_1780_cov_3.129275, whole genome shotgun sequence.
ACCESSION   DAOQMF010000453 DAOQMF010000000
VERSION     DAOQMF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48319_length_1780_cov_3.129275"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1300
                     /locus_tag="PLV77_07740"
     CDS             113..1300
                     /locus_tag="PLV77_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC15775.1"
                     /translation="MKLSRPAVWSRFAVFTVLAFAAAFAVVGCGDNDDNGWDGQSITF
                     GALFSVTGDGSPFGPQQVKGAKLAVDEINADGGINGADLEVVQRDDESDPATSADEMK
                     ELIEQVQAMAVLGPTFSNSAAEADPIANQNKTPVLAVSNTGPGIVGDCDYPCEYIFRD
                     SLGEATAIPANVKNLVAREHPEYAKVLYPADDPFGQTSAETAVQAFKDEGVKVVATQV
                     GADGITSMTDERRDAFMITASSGETGAGIVKDLRNAYGAKTPILGGNAFNSPVVAESL
                     GQQGKGVQSAAAWYAGNHSDENHEFMANYMAKYGQEPDQFAAQAYTGVKLLADAAEEA
                     DLQFGDIAADREALKTALEGVSEETPLGDFSFTPDHDVSQPIWIVQMNGKGTYDLVAQ
                     VNP"
     gene            complement(1504..>1780)
                     /gene="rpsG"
                     /locus_tag="PLV77_07745"
     CDS             complement(1504..>1780)
                     /gene="rpsG"
                     /locus_tag="PLV77_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNC15776.1"
                     /translation="IKQLTPALEVRSRRVGGATYQVPVEVPSRRARTLAVRWLVDFAR
                     ARNEYSMSERLANEILDATKQQGNAYKRKDDIFRMAQANKAFAHYRW"
BASE COUNT          362 a          570 c          543 g          305 t
ORIGIN      
        1 ttggaggact ggcacttcgc gccctgaatg aagaacaact cagatcgatc ggaccgcgga
       61 tcacgggaga taccgctgcc aaagcgtccg ctgacaagga gaaaaccacc tgatgaagct
      121 gtctcgtccc gctgtttggt cccgatttgc cgtcttcacc gtcctcgcct tcgccgccgc
      181 ctttgccgtg gtcggatgcg gtgacaacga cgacaatggc tgggacggcc agtcgatcac
      241 cttcggtgcc ctgttctcgg tcaccggtga cggctcgccc ttcggtccgc agcaggtcaa
      301 gggagcaaag ctggcggttg acgaaatcaa cgccgacggc ggcatcaacg gagccgacct
      361 cgaagtggtc cagcgtgacg acgaatcgga tccggccact tccgccgacg agatgaagga
      421 actgatcgag caggtccagg cgatggccgt cctcggcccc acattctcca actcggctgc
      481 cgaagccgat ccgatcgcca accagaacaa gaccccggtc ctcgcggtct ccaacaccgg
      541 ccccggaatc gtcggcgatt gcgattaccc ctgcgaatac attttccgcg actcgctggg
      601 tgaagccacc gcgatcccgg ccaacgtgaa gaacctggtc gcccgtgaac atccggaata
      661 cgccaaggtc ctttacccgg cagacgatcc cttcggccag accagcgccg aaaccgcagt
      721 tcaggcgttc aaggacgagg gagtcaaggt tgtcgccaca caggtcggcg ccgacggcat
      781 cacttcgatg accgacgaac gacgggacgc cttcatgatc accgcttctt ccggcgagac
      841 cggggccggg atcgtcaagg accttcgtaa cgcctacggc gccaagactc cgatcctcgg
      901 cggcaatgcc ttcaactcgc cggtcgtcgc cgagagcctc ggccaacagg gcaagggcgt
      961 ccagtccgct gccgcctggt acgccggcaa tcactcggat gagaaccatg agttcatggc
     1021 gaattacatg gcgaagtacg ggcaggaacc ggaccagttc gccgcccagg cctacaccgg
     1081 ggtcaagttg ctggccgacg cggcggagga agccgatctc cagttcggtg acatcgccgc
     1141 cgaccgcgaa gccctcaaga ccgccctgga aggggtcagc gaagaaaccc cgctcggtga
     1201 cttcagcttc acccccgacc acgacgtaag ccagccgatc tggatcgtcc agatgaacgg
     1261 caagggcacc tacgacctgg tcgcacaggt caatccctaa ccgaactccg acccctcgac
     1321 acgaagtggg tatctatttc gtctatatag acgaaatcca tagtcacctc gctcctggcg
     1381 agcgggaaat ccgaggtggg tatgagattc gttcatatgg acgaaatcca tagtcacctc
     1441 actggtctgc aatgaaaagg gcggctcggt ggccgcccct ttcgcaagtc ttgatttcgc
     1501 ctgctaccag cggtagtggg cgaaggcctt gttggcctgg gccatgcgga agatgtcgtc
     1561 cttccgcttg taggcgttgc cctgctgctt ggtcgcgtcg aggatctcgt tggcgagacg
     1621 ctcggacatc gagtactcgt tgcgggcgcg ggcgaaatcg accagccagc ggaccgcgag
     1681 ggtgcgggcg cgccgggagg gaacctcgac cgggacctgg taggtggcgc caccgacgcg
     1741 gcgggagcga acctcaaggg ccggggtgag ctgcttgatt
//
LOCUS       DAOQMF010000454         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_48818_length_1770_cov_3.662974, whole genome shotgun sequence.
ACCESSION   DAOQMF010000454 DAOQMF010000000
VERSION     DAOQMF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48818_length_1770_cov_3.662974"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLV77_07750"
     CDS             complement(<1..383)
                     /locus_tag="PLV77_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNC15777.1"
                     /translation="MDAVREVFENSIDFTIGLEEEFAIVDPDSLELVDRYPDLYAAAQ
                     DDPVLAGSAAGELIASEIEIRSGRSETMSEATKRQEEKRRHLFRLAEAHSVALASLGT
                     HPWADYLDQEIIDTPHYQRLKKDLG"
     gene            complement(409..1077)
                     /locus_tag="PLV77_07755"
     CDS             complement(409..1077)
                     /locus_tag="PLV77_07755"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNC15778.1"
                     /translation="MSQTPKSRTDNPLLVGEGERLALGVAARLSRYLQVLIQARKMGR
                     ETISSQELSEYTHINPTQIRRDLSGFGKFGKRGVGYRIDYLIDEIRKIMRTAGQHNIA
                     LFGAGKLGQAIAGSDVFADHGFRIVSLFDVDPEMIGKEVGQLTVRDFAELGEAVQEEA
                     IVVGVLAVPSAAAQEVADGLVEAGVTVIFNYTEQLLQVPPKVTVHTSSPAVDLLYALY
                     FYLS"
     gene            complement(1099..1506)
                     /locus_tag="PLV77_07760"
     CDS             complement(1099..1506)
                     /locus_tag="PLV77_07760"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNC15779.1"
                     /translation="MHRQRGDDRQRRQVQPAARLSGLPLFRRFLDRPPGQSLTGAGRT
                     FEVTFLSRPGCHLCDHALAELNEYLAGRGGDGQAVPGVIVEVINIDTDDELHRRFLER
                     IPVILVGERIVSEFSFEPEALESVLGDCSQGAA"
     gene            complement(1392..>1770)
                     /locus_tag="PLV77_07765"
     CDS             complement(1392..>1770)
                     /locus_tag="PLV77_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNC15780.1"
                     /translation="SGLKTALVYISRELGEEETARRAADLAAGYQAAVVDQLVAKLRR
                     ALEAGEWPAVALGGGVAANGPLRRAVAGLCEQKGIPFKEVNRELCTDNAAMIASAARF
                     SQPLAYPDYLSFDAFSTGPRGNR"
BASE COUNT          319 a          574 c          567 g          310 t
ORIGIN      
        1 cagccgaggt ccttcttcaa ccgctggtaa tggggggtgt cgatgatctc ctggtcgaga
       61 tagtcggccc aggggtgggt cccgagtgaa gccagggcga ccgagtgggc ttcggccagc
      121 cggaagagat ggcggcgctt ctcctcctgc cgtttggtcg cctcgctcat cgtttcggac
      181 cggcccgagc ggatttcgat ctccgaggcg atcagctccc cggcggcgga cccggccagc
      241 accgggtcat cctgggccgc ggcgtagagg tccggatagc gatcgaccag ttccaggctg
      301 tccggatcga cgatcgcgaa ctcttcctcg aggccgatcg tgaaatcaat cgagttctcg
      361 aagacctccc gcacagcatc catttccagc aggccgtggt cggacaaatc aggagagata
      421 gaagtagagg gcgtaaagca ggtcgaccgc cgggcttgag gtatggaccg tgaccttggg
      481 cggcacctga aggagttgct cggtgtagtt gaagatcacg gtcacgcctg cttcgaccag
      541 gccgtctgcg acctcctggg cggcagccga aggcacggcc agcaccccga ccacgatcgc
      601 ctcctcctgg accgcctcac cgagctcggc gaaatcgcga accgtgagct ggccgacctc
      661 tttgccgatc atttccggat caacgtcgaa aagggaaacg atgcggaagc cgtgatcggc
      721 gaacacgtcc gaaccggcga tcgcctgacc gagcttgccc gcaccgaaca gcgcgatgtt
      781 gtgctggccg gccgtccgca tgatcttgcg gatctcgtca atcaggtagt cgatccggta
      841 ccccaccccg cgtttgccga atttcccgaa gccggaaagg tcgcggcgga tctgggtcgg
      901 gttgatgtgg gtgtactcgg agagttcctg cgaggagatg gtctcgcgac ccatcttccg
      961 ggcctggatc agcacctgca ggtagcgcga aagccgtgcc gcgaccccga gagcgaggcg
     1021 ctccccttcg ccaaccagca gcggattgtc ggtgcgggat ttgggagttt gtgacatcgg
     1081 gctacaagcg tacccgtctc aggcagcgcc ctgtgaacag tcgcccagga ctgactccaa
     1141 agcttcaggt tcgaagctga attcagagac gatgcgctcg cccaccagga tcaccgggat
     1201 ccgttccaga aaccggcgat ggagctcatc gtcggtatcg atgttgatca cttcgacaat
     1261 caccccggga acagcttgcc catccccgcc gcgtccggcg agatactcgt tcagctcggc
     1321 cagggcgtga tcacaaaggt ggcagccggg acggctcagg aaggtgactt cgaaagtccg
     1381 gcccgctccg gtcaacgatt gccccggggg ccggtcgaga aagcgtcgaa agagaggtag
     1441 tccggatagg cgagcggctg gctgaacctg gcggcgctgg cgatcatcgc cgcgttgtcg
     1501 gtgcaaagtt cgcggttcac ctccttgaag gggatcccct tctgctcgca cagcccggcc
     1561 accgcgcggc ggagcggccc gtttgccgcc acaccaccac ccagggcgac cgccggccat
     1621 tccccggcct ccagggcgcg cctgagcttg gccaccagct ggtcaaccac ggcagcctgg
     1681 tagccggcag ccagatcggc ggcccgcctt gcggtctctt cctcccccag ttcccggctg
     1741 atgtagacca gcgccgtctt caggccgctg
//
LOCUS       DAOQMF010000455         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_48929_length_1768_cov_3.828955, whole genome shotgun sequence.
ACCESSION   DAOQMF010000455 DAOQMF010000000
VERSION     DAOQMF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48929_length_1768_cov_3.828955"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLV77_07770"
     CDS             complement(<1..897)
                     /locus_tag="PLV77_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15781.1"
                     /translation="MSNPDEREDRNIPDPVELKLYRGRIAEAASRAALEKEPDPAGIW
                     AEAAGPGSYTWLWTTGIKRAPTVLGWAALVICLLATLAGFVDIPLFTGELLAAPQMPV
                     FSSVTLGMVAFALLAMRPGAAESAVQAGVALCYFTIAVCMALLALRMVGIDPDVWGSD
                     ALRSPSVAALVNNPALALCLLTIDRDPPRYRWQRALIPLVGIIFLISVLTKGYQAALD
                     ANVQTDISILGSATVILIYVGYVFLRPDRGLAGFLSSTGPGPSMARLLVPTAVVIPLT
                     MVMVDLISEGLEPGGIQFGRPGV"
     gene            complement(983..>1768)
                     /locus_tag="PLV77_07775"
     CDS             complement(983..>1768)
                     /locus_tag="PLV77_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC15782.1"
                     /translation="GHVRLGHNAKVGYLSQHAETATGDGTVLDAAARETGLTGQKVRD
                     LLGAFLFSGEDANKQLSDISGGEQRRLSLAILVASGANLLLLDEPTNHLDIESREALE
                     DALLGFPGAVVLISHDRALLEAVGSRTLVCENGELTNHMTGWAEYQKRRDEAAEAEQE
                     KAAASRPAGKSGNARRGGSPGKEVARTRRRVENLEKKIEQAEARLAEIEDELSDPAAW
                     STPEKSEKATRRHDRAKEKVASLYAEWEEADQVARETAEAATA"
BASE COUNT          289 a          599 c          592 g          288 t
ORIGIN      
        1 cacccccggt cggccgaact ggatgccgcc gggctcgagc ccttccgaga tcaggtcgac
       61 catgaccatg gtcagcggga tcaccaccgc cgtcggaacg agcagccggg ccattgacgg
      121 tccgggaccg gtcgatgaga gaaaaccggc cagcccccgg tcaggccgga ggaacacgta
      181 cccgacgtag atcaggatta cggtcgccga tccgaggatg ctgatgtcgg tctgaacgtt
      241 ggcatcgagc gcggcctggt aacccttggt caggaccgag atcaggaaga tgatgccgac
      301 cagcgggatc agggcccgct gccagcggta gcggggcgga tcgcgatcga tcgtcagcag
      361 gcagagtgcc agggccgggt tgttgacgag cgcggcgacc gacggcgagc ggagcgcgtc
      421 cgaaccccac acgtccggat cgataccaac catccgcagg gccagcagcg ccatgcagac
      481 ggcgatcgtg aagtagcaga gcgccacgcc ggcctggacc gccgattcgg cagcgccggg
      541 gcgcatcgcc agcagggcga aggcgaccat tcccagggtc accgacgaga acaccggcat
      601 ctgcggagcg gcgagaagct cgcccgtgaa caacgggatg tcgacgaaac ctgccaaggt
      661 cgccagcagg cagatcacca gggcggccca gccgagcacg gtcggggccc gcttgatccc
      721 ggtggtccag agccaggtgt agctgccggg gccggccgct tcggcccaga tcccggcggg
      781 gtcgggctcc ttttcgagcg ccgcccggct cgcagcctcg gctatccgcc cccggtacag
      841 cttcagctcg accggatcgg gaatgttccg atcttcccgc tcatctggat tcgacaagtg
      901 cttctccaaa ggaactcctg actgcgaaac taacacctgc tttcccggga acgggaacgc
      961 cgaaacgcat ggtggccgcg gttcaggcgg tggcggcttc ggccgtctcc ctggcgacct
     1021 gatcggcctc ttcccactcg gcgtagagcg aggctacctt ctccttcgcc cggtcatgcc
     1081 gccgggttgc cttctccgac ttctcgggag tcgaccaggc cgcgggatcg gagagttcgt
     1141 cctcgatttc agccagccgg gcctcggcct gttcgatctt cttttcgagg ttctcgaccc
     1201 ggcgccgggt tcgggccacc tccttgccgg gcgagccgcc gcgccgggcg ttgcccgact
     1261 tcccggccgg ccgggaagcg gcagcttttt cctgttcggc ctccgcggct tcatcccggc
     1321 gcttctgata ctccgcccag ccggtcatgt ggtttgtcag ttcgccgttc tcgcagacca
     1381 gggtccggct gccgaccgct tccagcaggg cgcgatcgtg agaaatgagg acgaccgccc
     1441 cggggaaacc gagcagggca tcctcgagtg cttcccggct ttcgatgtcc agatggttgg
     1501 tcggctcgtc aagcagcagg aggttggctc cggaggcgac caggatcgcc agcgagagcc
     1561 gccgttgctc accgcccgag atgtcgctca gctgcttgtt cgcgtcttcg cccgagaaaa
     1621 gaaaggcgcc gagcaggtcc cggaccttct ggccggtgag cccggtttcg cgggcggccg
     1681 cgtcgagcac ggtgccgtcg cccgtagcgg tctccgcgtg ctgcgagagg taacccacct
     1741 tggcgttgtg accaagccgg acatgccc
//
LOCUS       DAOQMF010000456         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_48994_length_1767_cov_3.630257, whole genome shotgun sequence.
ACCESSION   DAOQMF010000456 DAOQMF010000000
VERSION     DAOQMF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48994_length_1767_cov_3.630257"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..>1767)
                     /locus_tag="PLV77_07780"
     CDS             complement(199..>1767)
                     /locus_tag="PLV77_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNC15783.1"
                     /translation="VKGYKIPGGTPSSPKLAMTLPAFPSNLKKQLKGAKYPAPIAIMS
                     AAVEGSQVDFDTAIEIEGRYFLELATGQIAKNMIQAFFFDLQEVNGDRGRPADGERYT
                     AKKVVVLGAGMMGAAIAYVCAKAGIEVVLKDISLEAAERGKGYSKDIVDKAISRGRST
                     QEKGDALLALITPTAEAKDADGADLVIEAVFEDPAVKAQVFAEIEPFLAEGALLGSNT
                     STLPITGLAEGVSPPADFIGLHFFSPVDKMPLLEIIKGEETSDTTLHRSLDLARQIRK
                     TPIVVNDSRGFFTSRVIGTFINEGIAFLAEGVPASTIEQASSQAGYPAPVLQLSDELN
                     MELMIKIRKATREALEAEGVEWNPHPAFAVVDRMVEAGRAGKLAGAGFYDYEDGKRTG
                     LWAGLREEFPPAGDPSQIDLLELQERMLFVESLETVKCLDEGVIESVADANIGSIFGI
                     GFPGWTGGVLQYIDGYTSEAHPERRGPAGFIERSRELAEKYGERFEPPASLVEKAERG
                     ETYSGQPVRDLVAS"
BASE COUNT          275 a          592 c          592 g          308 t
ORIGIN      
        1 ggccctgagg atgttctccc cggccggcag gtaaccgcgg cccgcggccg attcgccccg
       61 gaggaagtcc cccatcgagg cgatctgccc ggaaaccggt tcgagcgcct cggcccagcc
      121 ggggtcgatt acctgactta gcggacgggc cggcacgaac ccagaatacg agttcgcgcc
      181 ggcgccgccc gcggaaggtc aggaagcgac cagatcccgg accggctggc cggagtaggt
      241 ctcgccacgc tcggcctttt cgaccaggga agccggcggc tcgaaacgct cgccgtactt
      301 ttcggccagc tcccggctgc gctcgatgaa gcccgccggg ccgcggcgct cggggtgggc
      361 ttccgaggtg tagccgtcga tgtactggag cacgccgccg gtccagcccg ggaagccgat
      421 cccgaagatc gagccgatgt tggcgtcggc caccgattcg atcacgccct cgtcgaggca
      481 cttgaccgtt tccagcgatt caacgaaaag catccgctcc tgcagttcga gcaggtcgat
      541 ctgcgagggg tccccggccg gagggaactc ttccctgagg ccggcccaga gcccggtccg
      601 cttgccgtcc tcgtagtcgt agaaaccggc acccgccagc ttcccggccc gacccgcttc
      661 gaccatgcgg tcgaccacgg cgaaggccgg gtgcgggttc cactcgacgc cctcggcttc
      721 cagggcctcg cgggtcgcct tgcggatctt gatcatcagc tccatgttga gctcgtcgct
      781 cagctggagc accggggccg ggtagccggc ctggctcgaa gcctgctcga tggtcgaggc
      841 cggcacgccc tcggcgagga aggcgatgcc ctcgttgatg aaggtgccga tcacccggct
      901 ggtgaagaag ccgcgtgaat cgttgaccac gatcggtgtc ttcctgatct gccgggcgag
      961 gtcgagcgag cggtggagcg tcgtatcgct ggtctcttcg cccttgatga tctccagcag
     1021 gggcatcttg tcgaccgggc tgaagaagtg aaggccgatg aagtcggccg ggggtgaaac
     1081 gccctcggcc agaccggtga tcggcagggt cgaggtgttc gagccaagca gtgcgccttc
     1141 ggccaggaag ggctcgatct cggcgaagac ctgggccttg accgccgggt cctcgaagac
     1201 cgcctcgatc accaggtcgg cgccgtcggc gtccttcgct tcggcggtcg gggtgatcag
     1261 ggccagcagg gcatcgccct tctcctgggt cgaacggccg cgggagatcg ccttgtcgac
     1321 gatgtccttc gagtaaccct tgccccgctc ggcggcctcc agcgagatgt cctttagcac
     1381 cacctcgatg cccgccttgg cgcagacata ggcgattgcg gcgcccatca ttccggcacc
     1441 cagcaccacc actttcttcg cggtgtaccg ctcgccgtcg gcgggacggc cgcggtcacc
     1501 gttcacttcc tgaaggtcga agaagaatgc ctggatcatg ttcttggcga tctggccggt
     1561 ggccagctcg aggaagtaac ggccctcgat ctcgatcgcg gtgtcgaagt cgacctgcga
     1621 accctcgacc gcggccgaca tgatcgcgat cggcgccggg tacttcgcac ccttgagctg
     1681 cttcttcagg ttggaaggga aagccggcag ggtcatcgcc agcttcgggc tggacggggt
     1741 gccgccgggg atcttgtagc ccttcac
//
LOCUS       DAOQMF010000457         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49010_length_1767_cov_2.867407, whole genome shotgun sequence.
ACCESSION   DAOQMF010000457 DAOQMF010000000
VERSION     DAOQMF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49010_length_1767_cov_2.867407"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLV77_07785"
     CDS             <1..819
                     /locus_tag="PLV77_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC15784.1"
                     /translation="IDPVSLNLDPSAAEAAITGATVGLLPIDIFGQPAELPAFERIAR
                     SRGLGILEDSCQALGAIDSEGRKVGSRGNVSTFAFYANKQMTTGEGGIVIPTDAGMAK
                     VLRSERNQGRAIDMDWLDHDRLGFNYRMSDVTAAIGVAQLGRLDELLAGRARVAGLYL
                     DRLAGVEGLELPAPDRPPAKRSWFVFPVRLPEGSDRDGVIRKLGEQGIPAKAYLPSIH
                     LFPHLAELGYREGQFPVAESVAARSLALPFHAGLSEGDIDRVASALAEALGSGA"
     gene            828..>1767
                     /gene="argH"
                     /locus_tag="PLV77_07790"
     CDS             828..>1767
                     /gene="argH"
                     /locus_tag="PLV77_07790"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNC15785.1"
                     /translation="MARHTDKSTSRFREPPDPRFWRLNRSIDFDRHLAPFDVAQSRAH
                     AAALNRIGVIADDEIKVIDEGLAQIAEEPKAGEFSFEQGDEDIHMAVERRLGELVGDL
                     AGKLHTGRSRNDQVATDICMAVMDAADGARQRIATAMNGLLQLAESHRDWTMPGYTHL
                     QRAQPVYLGHHLLSWFWMLSRDFERFAAARRAAAVMPLGAGALAGVNWEIDRRRVAAD
                     LGFESVTVNSLDSTSNRDMVLDYLSAGSICLTHLSRIGSELVIWSSTEFSFCQLSEPF
                     TSGSSIMPQKQNPDSAELLRAKAPRVIGDFTTLLGVL"
BASE COUNT          281 a          609 c          580 g          297 t
ORIGIN      
        1 attgacccgg tttctctcaa cctggatccg tccgccgccg aggcggcgat aaccggagcg
       61 acggtcggac tcttgccgat cgacatcttc ggccagccgg ccgagctacc ggcgttcgag
      121 cgaatcgccc ggtcccgtgg cctcggcatt ctcgaggact cctgccaggc gctcggagca
      181 atcgattcgg aaggccgcaa ggtgggcagc cgcggcaacg tctcgacctt cgctttctac
      241 gcgaacaagc agatgaccac cggagagggt ggcatcgtga tcccgaccga cgccggcatg
      301 gccaaagtgc tgagaagcga gcggaaccag ggccgggcga tcgacatgga ctggctggac
      361 cacgaccggc tcggcttcaa ctaccggatg tccgatgtga ccgccgcaat cggcgtcgcc
      421 cagctcgggc gcctggatga actgctggcc ggccgcgccc gggtcgccgg gctctacctc
      481 gaccgcctgg ccggggtaga gggcctcgag cttcccgccc cggaccggcc accggcaaag
      541 cgcagctggt tcgtgttccc ggttcgactt cccgaagggt ccgaccgtga cggggtgatc
      601 aggaagctgg gggagcaggg gattcccgcc aaggcgtatc tgccctcgat tcacctcttc
      661 ccgcatctgg ccgagctggg ttaccgcgag ggccagttcc cggtcgccga atcggtcgcg
      721 gcccgttccc tggccctgcc tttccacgcc gggctgagcg agggcgacat cgaccgggtt
      781 gcctccgcac tggccgaggc gcttggctca ggggcataat cagcgccgtg gccagacaca
      841 ccgacaagtc gacttcacgc ttccgcgagc cgccggatcc ccgtttctgg cggctgaacc
      901 ggtcgatcga tttcgaccgt catctggcgc ctttcgacgt cgcccagtcc cgggcccatg
      961 ccgcggccct gaaccggatc ggcgtgatcg ccgacgacga gatcaaggtg atcgacgagg
     1021 gcctggccca gatcgccgaa gaaccgaagg ccggcgagtt cagcttcgag cagggcgacg
     1081 aagacattca catggcggtc gagcgccgcc tcggcgagct ggtcggcgac ctggccggca
     1141 agctccacac cggccggtca cgcaacgacc aggtcgcgac cgacatctgc atggccgtga
     1201 tggatgcggc cgacggcgcg aggcagcgga tcgcgactgc gatgaacggg ctgcttcagc
     1261 tggccgaaag tcaccgcgac tggacgatgc ccggttatac ccacctgcag cgggcccagc
     1321 cggtctatct tggccaccac ctcctgtcct ggttctggat gctttcgcgc gatttcgagc
     1381 gcttcgccgc cgcccgcagg gctgcggcgg tgatgccgct cggggccggc gccctggccg
     1441 gggtcaactg ggagatcgat cgccggcggg ttgcggccga cctcggtttc gagtcggtca
     1501 cggtcaactc gctggattcg acctcgaacc gggacatggt gctggactac ctctcggctg
     1561 gctcgatctg cctgacccac ctttcgcgga tcggttccga actggtgatc tggtccagca
     1621 ccgagttctc cttctgccag ctcagcgagc ctttcacctc cggctccagc atcatgccgc
     1681 agaagcagaa cccggattct gccgagctgc tgcgagccaa ggcaccgcgg gtgatcggcg
     1741 acttcacaac gctgctcggc gtgctcc
//
LOCUS       DAOQMF010000458         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49038_length_1766_cov_3.748101, whole genome shotgun sequence.
ACCESSION   DAOQMF010000458 DAOQMF010000000
VERSION     DAOQMF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49038_length_1766_cov_3.748101"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLV77_07795"
     CDS             complement(<1..681)
                     /locus_tag="PLV77_07795"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNC15786.1"
                     /translation="MLLAIDVGNTQTHLGAFEGNELTRHWRFQTRAGATSDELAERIS
                     GLMELRGTGFDDLDGLVVSSVVPPLGSEYTRLAQQYLDVGCLLVGPGIRTGMSIEIDN
                     PAEVGADRLVNAIAAYDEVGDVCVGVDFGTGINFDAVSADGAYLGGAIAPGVEISLTA
                     LTERAARISRIDLGEPKKAIGTSSREAIQVGVLYGFAGKVDGIARRINRELGGDARFI
                     ATGGYAKSI"
     gene            725..1492
                     /locus_tag="PLV77_07800"
     CDS             725..1492
                     /locus_tag="PLV77_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNC15787.1"
                     /translation="MSKATLFSLPGSHPAVSVKLMLDYKGIDFKTIDLMPLMHKAVVR
                     AAGFPGTTVPALKFNGEKLQGSVDIARALDRIVPEPPLLPADEDARKQVLMAEAFGEA
                     ELQHAVRQILWNAVRRQPKSIATFLEGSSLPLPPKVAAYTSGPIIWGEYKVNESTDEN
                     VKANLIALPEWLDRLDAWVADGTLGGATPNAADFQIATSIRLAATLQDLRPFLENRPI
                     YEAAVRTAPVYAGDVPPGLPTEWLAPLRENAAAPVSD"
BASE COUNT          295 a          622 c          543 g          306 t
ORIGIN      
        1 gatcgacttg gcgtaaccgc ctgtcgcgat gaagcgggcg tcaccgccca gttcgcggtt
       61 gatgcggcgg gcgatgccgt cgaccttgcc ggcgaagccg taaagcaccc cgacctggat
      121 cgcctcgcgt gaggatgtgc cgatcgcctt cttcggttca ccgaggtcga tccgggaaat
      181 ccgcgccgcc cgttcggtca gcgcggtcag cgagatttcg acaccggggg cgatcgcgcc
      241 accgaggtag gcaccgtcag cagagaccgc gtcgaaattg atgccggtgc cgaaatcaac
      301 ccccacgcag acatcgccca cctcgtcgta ggcggcgatc gcgttgacca gccggtccgc
      361 accgacttcg gcggggttgt cgatttcaat tgacattccg gtgcggatac ccggtccgac
      421 caggagacaa cccacatcga ggtattgctg ggccagcctg gtgtactcgg agccgagcgg
      481 cggcacgacc gaggaaacga ccagtccgtc gaggtcgtcg aagccggtac cccgcagttc
      541 catcaggccg gatatccgtt cggccagctc gtcgctggtc gccccggccc tcgtctgaaa
      601 acgccagtga cgggtcagtt cgttgccctc gaaggctccg agatgggtct gggtgttgcc
      661 gacgtcaatt gccagaagca tgaacaaggg agtgtggcaa atgtcaagac ttatgatcct
      721 ccccatgagc aaggcgacgc tgttctcact tcccggttcc catcccgcgg tcagcgtgaa
      781 gctgatgctc gattacaagg gcatcgactt caagacgatc gacctgatgc ccctcatgca
      841 caaggccgtg gtgcgtgccg ccggcttccc cgggaccacg gttcccgccc tcaagttcaa
      901 cggcgagaag cttcagggtt cggttgacat cgcgcgggcg ctcgaccgga tcgtgccgga
      961 gccgccgctg ctgcccgccg acgaggacgc ccgcaagcag gtgctgatgg ccgaggcctt
     1021 cggcgaggcc gaactccagc atgcggtccg ccagatcctc tggaatgcgg tccgtcggca
     1081 gccgaagtcg atcgccacct tcctcgaagg ttcgagcctg cccctgccgc ccaaggtcgc
     1141 cgcctacact tccggcccga tcatctgggg cgagtacaag gtgaacgaat caaccgacga
     1201 gaacgtgaag gcaaacctga tcgcgctgcc ggagtggctc gaccgcctgg acgcctgggt
     1261 ggccgacggc acgctcggcg gtgccacccc gaacgccgcc gatttccaga tcgccaccag
     1321 catccgcctg gcggccaccc ttcaggacct ccgccccttc ctcgaaaacc gcccgatcta
     1381 cgaggccgca gtccgcaccg ctccggtcta cgcgggtgac gtccccccgg gcctgccgac
     1441 cgagtggctc gccccgctcc gcgaaaacgc cgccgccccc gtttccgact gagccccggc
     1501 cccggctccc gtttccgact gacccccggc ctcggcccgc gttccgactg agccccggcc
     1561 ccggctcccg atcgtcgagt gcccactttg tgccgcatac cggcacaaag tggaacttcg
     1621 cggccttccg gctctccggg tttgcggtcc tctagatcgt ggcgaggtcg gtgtcggggt
     1681 cggcgagggc gacgaggtcg atctgccgct ttgacttgat cagctcgccg atcgtctcgc
     1741 tgacgtccca gatattcacg ttcatt
//
LOCUS       DAOQMF010000459         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49060_length_1766_cov_2.661017, whole genome shotgun sequence.
ACCESSION   DAOQMF010000459 DAOQMF010000000
VERSION     DAOQMF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49060_length_1766_cov_2.661017"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PLV77_07805"
     CDS             complement(<1..1190)
                     /locus_tag="PLV77_07805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNC15788.1"
                     /translation="MWRRLVLAVIGRLRKGRIEFIEGGRVTAIGEPGAEPLARVEVHS
                     PRAWRRMVRGSTGMAEGYIKREWDSDDLVALGTIAGLNLGQLDRIKRRFRFILWPFQK
                     LGPLMPRTTRRRGRKQIAAHYDLGNDLFSLFLDESMNYSAAVFRGDPDQTLGQAQIAK
                     MDQIADQLDLSPDTHLLEIGTGWGGLSIHLAGRSGCRITTTTISREQASLARERIAAA
                     GLSERIEVVETDYRDLTGSFDRLVSIEMIEAVGWRDFPTYFAKCSELLTEDGSMLLQA
                     ITIDDDAYELEKASRSFISRYIFPGGCLPSIREIRRCLDQVTDLTIAWSDELRLDYAR
                     TLAEWRRRFNAVTGRLESLGYDDRFRRMWVMYLCLAEAGFRSGRNSDFQLLLTKPRSP
                     RVVAL"
     gene            complement(1193..>1766)
                     /locus_tag="PLV77_07810"
     CDS             complement(1193..>1766)
                     /locus_tag="PLV77_07810"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNC15789.1"
                     /translation="LTHLRYFGVCFNPVTFYYCFDPFGQRLESVLAEVTNTPWGERHS
                     YLVNRQRRPGVLAADVAKRLHVSPLMAMDHRYELVFAEPGPTLSVHMASRRKGHLHFD
                     ATLRMERQQLTRAKLGRLLLTRLPMPAKVLGGIHAEAARTWLAGARYHPHPEKTSRED
                     LPNRSKDGNLCPVDHSSPTRPQAERRRGEG"
BASE COUNT          298 a          560 c          610 g          298 t
ORIGIN      
        1 agagcgacga ctctcggcga acgcggcttg gtcaggagca gctggaagtc gctgtttcgg
       61 ccggaacgga aaccggcctc ggccaggcag agatacatca cccacatgcg gcggaagcga
      121 tcgtcgtagc cgagcgattc gagacggccg gtcaccgcat tgaaccggcg ccgccactcg
      181 gcgagggtgc gggcatagtc aaggcggagc tcgtcgctcc aggcgatggt caggtcggtc
      241 acctggtcga gacagcggcg gatttcccga atcgagggca ggcaaccgcc gggaaagatg
      301 tagcggctga tgaaactccg tgacgccttc tccagctcgt aggcgtcgtc atcgatcgtg
      361 atcgcctgaa gcagcatcga gccgtcttcc gtcagcagct ccgagcactt ggcgaagtag
      421 gtcgggaagt cgcgccagcc gaccgcctcg atcatctcga tcgagaccag gcggtcaaag
      481 cttccggtca ggtcgcggta gtcggtttcc accacttcga tccgttcgga gagacccgcc
      541 gccgcgattc gctcgcgggc caggctggcc tgctcccggg agatggtcgt ggtggtgatg
      601 cggcagccgg aacgcccggc caggtggatc gagaggccgc cccagccggt accgatctcg
      661 agcaggtggg tgtccggcga gaggtcgagc tggtcggcga tctggtccat cttcgcgatc
      721 tgggcctgtc cgagggtctg gtcgggatca ccccggaaga ccgccgcgga gtagttcatc
      781 gattcgtcca gaaacagcga gaagaggtcg ttgccgaggt cgtagtgggc ggcgatctgt
      841 ttccttcccc tccggcgggt cgttctcggc atcagggggc cgagtttctg gaagggccag
      901 aggatgaaac ggaagcgtct cttgatccgg tccagctgac cgagattgag gccggcgatc
      961 gtgcccagcg cgaccaggtc gtcgctgtcc cactcccgct tgatgtaccc ctcggccatc
     1021 ccggtgctgc cccggaccat ccgccgccag gccctgggcg agtggacctc gacccgggcc
     1081 aggggctccg cgccgggctc cccgatcgcc gtcaccctgc cgccttcaat gaattcgatg
     1141 cggcccttcc ggaggcgacc gatcaccgcc agcacgaggc gacgccacat gatcagcctt
     1201 cgccccttcg cctttcggct tggggccggg tgggagaaga gtggtccaca ggacagagat
     1261 tgccgtcttt cgaccgattc ggcaagtcct cccgggaggt cttctccggg tgggggtggt
     1321 aacgggcccc ggccagccag gtccgggccg cttcagcgtg gatgccgccc aggaccttcg
     1381 ccggcatcgg caaccgggtg aggagcagcc gaccgagctt tgccctggtc agctgttgtc
     1441 gctccatccg cagggtggca tcgaaatgga ggtggccttt cctccggctc gccatgtgga
     1501 ccgagagcgt cgggccgggc tcggcgaaga cgagttcgta gcggtggtcc atcgccatca
     1561 gcggcgaaac gtggagccgt ttggccacgt cggcggcgag cacaccgggc cggcgctgcc
     1621 ggttcacgag gtagctgtga cgctcacccc agggagtgtt cgtgacctcg gccagcaccg
     1681 actcaagccg ctgcccgaaa gggtcaaagc agtagtagaa ggtcaccggg ttgaagcaga
     1741 ccccgaagta gcgcagatgg gtcagc
//
LOCUS       DAOQMF010000460         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49086_length_1765_cov_3.951462, whole genome shotgun sequence.
ACCESSION   DAOQMF010000460 DAOQMF010000000
VERSION     DAOQMF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49086_length_1765_cov_3.951462"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..477
                     /locus_tag="PLV77_07815"
     CDS             127..477
                     /locus_tag="PLV77_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15790.1"
                     /translation="MASTELTSGARTLIPFRRLAAATAFATFALIVVGGIVRVSESGL
                     GCGPAGSGLEGWPLCTGRVVPSVGGATLVEFTHRVLAGLVAVLVVLLPGRLSATCATG
                     PGWSGALPSRSCWC"
     gene            438..1121
                     /locus_tag="PLV77_07820"
     CDS             438..1121
                     /locus_tag="PLV77_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNC15791.1"
                     /translation="MVRGSAFTVVLVLIQAGLGGLTVEHNLHATLVAVHLGIAMILLA
                     LLLCMYRAAHPGPQPEAGRASAGLRATAAIAAFLVLATIVVGGYVAGTEGEGTADKPS
                     GGAHTACGTDFPTCGDELLPYGRDSMVDAQLTHRVLMFAASVSVLAMLALALMRGIRG
                     WPAITAGGLVLLQVLLGALNIWLGKHAGLIIGHLTLGTLVWAVVVWAGITYTRFPGPE
                     RSGAPPRPA"
     gene            1118..>1765
                     /locus_tag="PLV77_07825"
     CDS             1118..>1765
                     /locus_tag="PLV77_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15792.1"
                     /translation="MSGGPVGEETACPVVRDATGAWVLRRHEDVLAAALDPQRFSSRV
                     SRHLNIPNGMDGEQHARYRKLVERYMSPERTDRLEGRFREIARELVDSLPRDRPLDAV
                     SGVGSRFAVRAQSAWLGWPADAEESLLEWMDENRAAARSSSLEMSKAVADRFDRIIRA
                     LVEARRDPAGCPDTDVTAELIAARIDGRPLTVEEIVSILRNWTAGDLGTIAACIGV"
BASE COUNT          252 a          605 c          623 g          285 t
ORIGIN      
        1 gaccttgccc ctcagctcca tcgaatccat cacaccactc tagagcctgt ttgccggtgg
       61 aaaaccgcag gctggatgtg gttaaaccgc aatcccgagg agattctgcg aactatcctc
      121 ggcgggatgg ccagcacgga gctgacatcc ggggccagaa ccctgatccc tttcaggcgg
      181 ctcgcggccg cgaccgcatt cgccaccttc gcgctgatcg tggtcggtgg catcgtcagg
      241 gtgagcgagt cgggactggg ctgcgggccg gccggcagcg gactcgaggg atggccactc
      301 tgtaccggca gggtggtccc ttcggtcggg ggcgcgaccc tggtcgagtt cacccaccgc
      361 gtgctcgccg gcctggtcgc ggtgctggtg gtgctcctgc ctggcaggct ttccgcaacc
      421 tgcgcgaccg gccctggctg gtccggggct ctgccttcac ggtcgtgctg gtgctgatcc
      481 aggccggtct cggcgggctc accgtcgagc acaacctgca cgcgaccctg gtcgcggtgc
      541 atctggggat cgcgatgatc ctgctggcct tgctgctctg catgtaccgg gcggctcacc
      601 cggggccgca gccggaagcc ggaagggcga gcgccgggct cagggctaca gccgcgatcg
      661 cagcattcct ggtcctggcc accatcgtgg tcggcggata tgtcgccgga accgaaggag
      721 aagggaccgc ggacaagccg tccggcggcg cccacaccgc ctgcggcacc gactttccga
      781 cctgcggaga cgagcttctg ccctacgggc gcgacagcat ggttgacgcg cagctcaccc
      841 accgggtcct gatgttcgcg gcgagcgtgt ccgtgctcgc gatgctggcc ctggccctga
      901 tgcgaggcat tcggggctgg ccggcgatca ccgcgggcgg gctggttctg ctgcaggtgt
      961 tgctcggagc gctgaacatc tggctcggca agcacgccgg cctgatcatc ggacacctca
     1021 cgcttggcac cctggtctgg gcggtcgtgg tctgggccgg catcacctac accaggttcc
     1081 ccggcccgga aagatccggc gccccaccga ggccggcttg agcggcgggc cggtcggcga
     1141 ggagaccgca tgcccggtcg tccgtgatgc gaccggcgcc tgggttctgc gtcgacacga
     1201 ggatgttctc gccgccgccc tcgacccgca gaggttctcg agccgggtct cgcgccacct
     1261 gaacatcccc aacggcatgg atggcgagca gcacgcccgc taccggaagc tggtcgagcg
     1321 gtacatgagc ccggagcgaa ccgaccggct cgagggccgg ttccgcgaga tcgcgcgcga
     1381 gctggtcgac tcgctgccca gggacaggcc gctcgacgcg gtgtccgggg tcggcagccg
     1441 tttcgcggtt cgggcccaga gcgcctggct cggctggccg gccgatgccg aagagtcgct
     1501 gctcgaatgg atggatgaga accgtgcggc cgcccggtca tccagcctcg agatgagcaa
     1561 ggcggtggcg gaccgcttcg accggatcat ccgcgcgctg gtcgaagccc ggcgcgatcc
     1621 ggccggctgc cctgacaccg acgtgaccgc ggagctgatc gctgcgcgga tcgatggccg
     1681 gccactcacg gtcgaggaga tcgtcagcat ccttcgcaac tggaccgccg gcgacctcgg
     1741 cacgatcgcc gcctgcatcg gggtg
//
LOCUS       DAOQMF010000461         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49204_length_1763_cov_3.145199, whole genome shotgun sequence.
ACCESSION   DAOQMF010000461 DAOQMF010000000
VERSION     DAOQMF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49204_length_1763_cov_3.145199"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLV77_07830"
     CDS             <1..689
                     /locus_tag="PLV77_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC15793.1"
                     /translation="AESSLTARVRESIREAIIDGSLEPGSLHSVKSLADIFKVSRTPV
                     REALIDLAGADMVEFERNRGVRILETSVHDLEEILVLRILLEVPATYRAAEHIDADGL
                     SALRQELDAMVEAAENGDEPTMMQHDRRFHELINTASGNSRLTEYVDSLRDLILTRGV
                     STVDNTRSLTEIVDEHEGILTAIASGDGEAAAAAMKEHLVNTASLLLQQEGGRSSSLS
                     LGWERLVAGG"
     gene            686..1594
                     /locus_tag="PLV77_07835"
     CDS             686..1594
                     /locus_tag="PLV77_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC15794.1"
                     /translation="MSKLVKLAVASWRRQKLRTAMTVGSLALSVGAVVFVYSLSVAFE
                     SSGSQAIESAIGKADIWVVPAKGVEVNRKDESIDAIGSLPSGIEAEVRSLPAVTAVTA
                     VTGSGAGTLQVTTSNPKLTADAMKSKGLTVTSDPARSTATGDAPALAYLITASEDRFG
                     VYTFSQQFEAVQVNEVASSVLGVVGRVTLALGFLAVLTSLLISIDERRREFGILAAVG
                     ITDDVLYLFLVESGLLIAMGLVVGVIFGGGMFALLLPSIFSLGTVGKAVALVSVYFPI
                     MLILGALIPAWRLLQKSPLELLRSSP"
BASE COUNT          279 a          588 c          576 g          320 t
ORIGIN      
        1 aggctgaatc gagcctcacg gcgagggtgc gcgagtcgat tcgcgaggcg atcatcgacg
       61 gtagcctgga gcccgggtcg cttcactcgg tcaagtcgct ggctgacatc ttcaaggttt
      121 cccggacccc ggttcgcgag gccctgatcg acctcgccgg ggccgacatg gtcgagttcg
      181 agcgcaatcg cggtgtccgg attctcgaga cctcggtcca cgacctcgaa gagatcctcg
      241 tcctgcggat cctactcgag gttcccgcca cctaccgggc ggccgagcac atcgatgccg
      301 acgggctcag cgccctcagg caggagctcg acgcgatggt cgaggcggcc gagaacgggg
      361 acgagccgac catgatgcag cacgaccgcc gcttccacga actgatcaac acggcctccg
      421 gcaactcccg gttgaccgaa tacgtcgact cgcttcgcga cctgatcctg acccgcggag
      481 tttcgaccgt tgacaacacg cgctcgctga cggagatcgt cgacgagcac gaagggatcc
      541 tcaccgcgat tgcttccggc gatggtgaag cggcagcggc cgcgatgaag gagcacctgg
      601 tcaacacggc ctcgctcctg cttcagcagg agggcggacg cagctcgtcg ctgagcctgg
      661 gctgggagag gctggtcgcc ggcggatgag caagctggtc aagctcgcgg tcgccagctg
      721 gcgacgccag aagctccgca ccgcgatgac ggtcggctcg ctggcccttt cggtcggggc
      781 ggtcgtcttc gtctattcgc tttcggtcgc tttcgagagt tccggctccc aggcgatcga
      841 gtcggcgatc ggcaaggccg acatctgggt ggtgccggcc aagggggtcg aggtcaaccg
      901 gaaggacgag agcatcgacg cgatcggcag cctgccgtcg gggatcgagg ccgaagtcag
      961 atcgctgccg gcagtcactg ccgtgaccgc ggtcaccggc agcggtgccg gaacccttca
     1021 ggtcaccact tccaacccga agctgaccgc ggacgcgatg aagagcaagg gcctgaccgt
     1081 gacttcggac ccggctcgca gcacggcgac cggagacgcc ccggccctcg cctacctgat
     1141 cacggccagc gaggaccgct tcggcgtcta caccttctcc cagcagttcg aggcggttca
     1201 ggtcaacgag gtggcctcca gcgtgctcgg ggtggtcggc cgggtcaccc ttgcgctcgg
     1261 tttcctcgcg gtcctgacct cactgttgat ctcgatcgac gaacgccgcc gcgagttcgg
     1321 catcctcgcc gcggtgggga tcaccgatga cgtgctctac ctgtttctgg tcgaatccgg
     1381 actcctgatc gcgatggggc tggtggtcgg ggtgatcttc ggaggaggca tgttcgccct
     1441 gcttttgccc tcgatcttct cgctggggac ggtgggcaag gcggttgccc tggtctcggt
     1501 ctacttcccg atcatgctca ttctcggggc gttgatcccg gcctggcgcc tgcttcagaa
     1561 gagtcccctc gaactgctca ggagttcacc ttgatcaaac ccctcaagta cagcctgcgg
     1621 atgatcaccc gggcccgcgg ctcgtcgctg cttcgctccg gcgcggtggc cgtcgcggcc
     1681 gccctcacct tcttcgtcct cgcccagagt tcgaccgttc gcaatcaggc caccgatctc
     1741 gcggtcgaac tctgggcgag gac
//
LOCUS       DAOQMF010000462         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49397_length_1759_cov_2.910798, whole genome shotgun sequence.
ACCESSION   DAOQMF010000462 DAOQMF010000000
VERSION     DAOQMF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49397_length_1759_cov_2.910798"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLV77_07840"
     CDS             <1..651
                     /locus_tag="PLV77_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNC15795.1"
                     /translation="IALIGASGSGKSTLLRCIDLLVEIDDGDIFLDGEVITDPSVDPV
                     SVRRKLGFVFQAFNLFPHMKVIDNVTLGSVRAQGSGKKEAREEGRALLERFGLGGREE
                     DMPDELSGGQQQRVALCRALAGNPRALLLDEVTSALDPELVGDVLDAVRDLKQEGMTM
                     ILATHEMSFAREVADEVAFLHEGRIIEQGSPGQILSEPNRPETRKFLRRLLAAGRL"
     gene            complement(800..1216)
                     /gene="atpC"
                     /locus_tag="PLV77_07845"
     CDS             complement(800..1216)
                     /gene="atpC"
                     /locus_tag="PLV77_07845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNC15796.1"
                     /translation="MAGEHTFTVEVLTPEGEVFGGEVIQLSTRTVTGEIGILARHVPI
                     LAALKPNLLRLKVSDSEEKEWAQSHGMLQVFANHAQVLLEEAIEPANLDVPALEEQKA
                     DAEARLADDSTGEAAREVAERDLERVTAFLEIAQAA"
     gene            complement(1231..>1759)
                     /locus_tag="PLV77_07850"
     CDS             complement(1231..>1759)
                     /locus_tag="PLV77_07850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNC15797.1"
                     /translation="STARGSVTSVQAIYVPADDHTDPAPASVFAHLNATTALSRAISE
                     KGIYPAVDPLDSSSTILKPDILGEEHYNTATEVQQVLQRYSELQQIIAILGMDELADE
                     DKVLVYRARKIQRFLSQPFFVAEQFTGSPGQYVPVAETVRGFRMILDGEMDEYAESAF
                     YMKGSIDQVIEQGKS"
BASE COUNT          283 a          567 c          605 g          304 t
ORIGIN      
        1 atcgccctga tcggcgcttc cggctccggc aagtcaaccc tgctgcgctg catcgacctg
       61 ctggtcgaga tcgatgacgg cgacatcttc ctcgacggcg aggtgatcac cgacccctcg
      121 gtcgatccgg tctcggtccg gcgcaagctc ggcttcgtct tccaggcttt caacctcttc
      181 ccgcacatga aggtgatcga caacgtcacg cttggctcgg tccgggccca gggatcgggg
      241 aagaaggaag cccgcgagga gggaagggcc ctgctggagc gtttcgggct cggcggccgc
      301 gaagaggaca tgccggacga gctttccggt ggccagcagc agcgggtcgc cctctgccgg
      361 gccctggccg gcaatccccg ggccctcctc ctggacgagg tgaccagcgc cctcgacccc
      421 gaactggtcg gcgacgtgct cgatgccgtc cgggatctga agcaggaagg gatgacgatg
      481 atccttgcca cccacgaaat gagcttcgcc cgcgaggtgg ccgacgaggt cgccttcctc
      541 cacgaaggcc ggatcatcga acagggaagc cccgggcaga tcctctcgga accgaatcgc
      601 cccgaaaccc gcaagttcct ccgccgcctc ctcgccgctg gccgcctcta gttggccgtg
      661 gggccgggtg gccctcccaa agtggcttcc cgattactgc tccggtcagc gagcaggtgg
      721 ggacaagcgc gacggtggcg ccatcttcgg gagggctgcc cgatcccgcg gcgtaaccac
      781 cgcggccggg aaaacgaagc taggcggcct gggcgatttc gaggaaggcg gttacgcgct
      841 cgaggtcgcg ctcggcgact tcacgggcgg cttcgccggt cgagtcgtcg gcaaggcggg
      901 cttcggcgtc ggccttctgc tcttcgagcg cggggacgtc gaggttggcg ggttcgatcg
      961 cttcctcgag cagcacctgc gcgtggttgg cgaagacctg gagcattccg tgggactgcg
     1021 cccactcctt ctcttcagag tcggagacct tcagccgcag caggttcggc ttgagcgcgg
     1081 cgaggatcgg cacgtgccgg gccagaatgc cgatctcgcc ggtgacggtc cgggtcgaga
     1141 gctggatcac ctcgccgcca aagacctcac cctcgggggt gaggacctca accgtgaatg
     1201 tatgttcgcc agccaacttc ctagaagccc ttagctcttg ccctgctcga tgacctggtc
     1261 gatcgagccc ttcatgtaga acgcgctctc ggcgtactcg tccatctcgc cgtcgaggat
     1321 catgcggaaa ccgcggaccg tttcggcgac cggcacgtac tggcccggag aaccggtgaa
     1381 ctgctcggcg acgaagaacg gctgcgacag gaaacgctgg atcttgcggg cgcggtacac
     1441 cagcaccttg tcctcgtcgg cgagttcgtc catgccgagg atggcgatga tctgctggag
     1501 ctcgctgtag cgctgcagga cctgctggac ctcggtcgcg gtgttgtagt gctcctcgcc
     1561 gaggatgtcc ggcttgagga tggtcgacga cgagtcgagc ggatcgacgg ccgggtagat
     1621 gcccttctcc gagatcgccc gcgaaagcgc ggtggtcgcg ttgaggtggg cgaagaccga
     1681 ggccggagcc gggtcggtgt ggtcgtcggc cgggacgtaa atcgcctgga ccgaggtgac
     1741 cgagccacgg gcggtcgag
//
LOCUS       DAOQMF010000463         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49436_length_1758_cov_3.671756, whole genome shotgun sequence.
ACCESSION   DAOQMF010000463 DAOQMF010000000
VERSION     DAOQMF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49436_length_1758_cov_3.671756"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..>1758
                     /locus_tag="PLV77_07855"
     CDS             73..>1758
                     /locus_tag="PLV77_07855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNC15798.1"
                     /translation="MTLALAGIGLGLAAPVTANAAPAADTVFRNGTVITIDRAQPKAQ
                     AVAVVGGRIAYVGSDRGAGKWIGKGTRVVNLKGRTMMPGLVDGHSHPLGGGDILDDCD
                     LGNIEATVEDMLDIMRDCDAADPATSNSDWLQVSNWSPVGVLPAGTVVTKADLDAAFP
                     DRPVYVQGSDFHNSWVNSRALELAGVTKDTPDPATGEFVRDDEGNPTGLLKDTAQDVV
                     KSAIPPKSFKESLADGRRAIKAITAVGITSATDAAADPGTLKVWKGLASKGQMPIRLN
                     SFVVVDAGQGVHGAAAYYKAMEKKYSTERLRVPGVKMFMDGVIEYPAQTAALIDPYLE
                     KVGDEWVPGSNRGDIYFTQKQADRFVTRFDRMNRRIHVHAIGDRAAKVALNAAAAARK
                     ANRSARRDNIAIAHLQLVDPSDYGRFAKLGVYADMQLQWAVSDFWTEDALHPFIGDER
                     YNRLYPARSLVKAGAPFAMGSDWPVDPLNPWNEIMTANTRRNDYGGLLLANQSIPVAT
                     ALRAHTLGSARQLGISGKVGSVTVGKDADLIVINRNTLKARPGSIDKTRVLRTI"
BASE COUNT          338 a          610 c          547 g          263 t
ORIGIN      
        1 ttgatcaagc caatctcgtc aaacagcagc aggatggagg caggaacccg catgaggact
       61 ggaattctca aattgaccct cgccctggcc ggcatcggcc tcggactggc cgccccggta
      121 accgcgaacg ccgccccggc cgcggacacg gtcttccgga acggcaccgt gatcacgatc
      181 gaccgggcac aaccgaaggc ccaggccgtc gccgtcgtgg gcggccgcat cgcctatgtc
      241 gggtccgacc gcggagccgg caagtggatc ggcaaaggca ccagggtggt caacctcaag
      301 ggccggacca tgatgccggg cctggtcgac gggcattccc atccgctcgg cggtggcgac
      361 atcctcgacg actgtgacct cgggaacatc gaagccacgg tcgaagacat gctggacatc
      421 atgcgcgact gtgacgccgc cgatccggcc accagcaact cggactggct tcaggtctcc
      481 aactggagtc cggtcggcgt gctccccgcc ggcaccgtgg tgaccaaagc cgatctcgac
      541 gctgccttcc cggaccggcc ggtctacgtc cagggttcgg acttccacaa ctcatgggtg
      601 aattcgcggg ccctcgagct ggccggcgtg accaaagaca cgcccgaccc cgcgaccggt
      661 gagtttgtcc gcgacgacga aggcaatccg accggcctcc tcaaggacac ggcccaggat
      721 gtggtcaagt cggccattcc gcccaagtcg ttcaaggaat ccctcgccga tggccgccgg
      781 gcgattaagg caatcaccgc agtcggcatt acctcggcca ccgacgccgc cgccgacccg
      841 ggcaccctga aggtgtggaa gggcctggcc agcaagggcc agatgccgat ccgcctcaat
      901 tccttcgttg tggtcgatgc cggccagggc gtacacggag ccgccgccta ctacaaggcg
      961 atggagaaga agtactccac cgaacgcctc cgggtgcccg gcgtgaagat gttcatggac
     1021 ggagtgatcg agtatcccgc ccagaccgcc gccctgatcg atccctacct cgagaaggtc
     1081 ggtgacgaat gggttccggg cagcaaccgc ggcgacatct acttcacgca gaagcaggcc
     1141 gaccggttcg tgacccgctt cgaccggatg aaccggcgga tccacgttca cgcgatcggc
     1201 gaccgggcgg caaaggttgc cctgaacgcc gccgccgcgg cccgcaaggc caatcgttcc
     1261 gcccgccggg acaacatcgc gatcgcccac ctccagctgg tcgacccgtc cgattacgga
     1321 cgcttcgcaa agctcggtgt ctacgccgac atgcagctgc agtgggcggt aagcgacttc
     1381 tggaccgaag acgccctgca tccgttcatc ggagacgagc gctacaaccg cctctacccc
     1441 gcccgcagcc tggtcaaggc aggggcgccg ttcgcgatgg gcagcgactg gccggttgat
     1501 ccgctcaatc cctggaacga gatcatgacc gccaacaccc gcaggaatga ttacggcgga
     1561 ctgttgctgg ccaaccagtc gattccggtg gccaccgccc tgcgggctca caccctgggc
     1621 tcggcccggc agctcggcat ttccggcaag gtcggttcgg tcaccgtggg caaggacgcg
     1681 gacctgatcg tgatcaatcg caatacgctc aaggccaggc cgggcagcat cgacaagacc
     1741 cgggtcctca ggacgatc
//
LOCUS       DAOQMF010000464         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49894_length_1750_cov_2.903835, whole genome shotgun sequence.
ACCESSION   DAOQMF010000464 DAOQMF010000000
VERSION     DAOQMF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49894_length_1750_cov_2.903835"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLV77_07860"
     CDS             <1..344
                     /locus_tag="PLV77_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15799.1"
                     /translation="FFYSANPNKAVKKGSYYAFRFPFVAFKTPRGIRIWGRTPNSKGG
                     KIRIQQNYGSGWRRKIVVRADKSGIFSKTFRSRRQVGRLRAIYGNSYSVPFSLKRVPD
                     RFVRPFGGLDD"
     gene            341..1444
                     /locus_tag="PLV77_07865"
     CDS             341..1444
                     /locus_tag="PLV77_07865"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC15800.1"
                     /translation="MSEKLPLFVRESSLAPLKPEIKRRVASVIDRGAYIQGEEVAAFE
                     QEFTNYLGREHCVGVANGTDALMIGLIALGVRPGDEVVVPAVTFFATAEAVAAIGAKP
                     VFADVELGTWTISKRTVEPLLTDSTAAIVPVHLFGNPAPMNELMSLADASGVPVLEDA
                     AQAAGATYDGKMAGAIGSAASFSFYPGKNLGAIGDAGALVTDDPEVADLARKLREHGS
                     ADRKIHTEVGFNSRLDSIQAAALSVFLPQLDAWTKSRRQAADAYGNSSLAGLVGLPVE
                     TAQAQSCHHLYVISSDRRDEILMALSAAEIESRVYYSPAIPFQPAMKAFAPDHQLPGA
                     TAYENSALALPMGESLSVEDAQRVAAVISSALS"
     gene            complement(1462..>1750)
                     /locus_tag="PLV77_07870"
     CDS             complement(1462..>1750)
                     /locus_tag="PLV77_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15801.1"
                     /translation="VRVYDHGVVFEDPETFGQYHLSYRTGDILTPKLPTTEPISAQLD
                     AFLDAVSAGRAPQKYTRIARDVVELIEMAERSLENEGVRSTLTKQLPAGLR"
BASE COUNT          358 a          522 c          518 g          352 t
ORIGIN      
        1 tcttcttcta ctcggccaat cccaacaaag ctgtcaaaaa gggctcgtac tacgccttcc
       61 gcttcccttt cgttgccttc aagacgcctc gggggataag gatctggggc cggactccga
      121 acagcaaggg cggcaagatt cggatccagc agaactatgg ttcgggctgg cggcggaaaa
      181 ttgtcgtcag ggccgacaag tccgggattt tcagcaagac tttccgaagt cgcaggcagg
      241 tgggtcgact tcgggcgatc tacgggaact catattccgt tccgttctcg ctaaagcgtg
      301 tgcctgatcg cttcgtcagg ccgttcggtg gactcgacga ttgagcgaaa agcttcccct
      361 tttcgttcgg gagtcttctc tcgcgccgct gaagcctgag ataaagcgac gggttgcttc
      421 ggtcatcgac cgtggtgcct acatccaggg cgaagaggtc gccgcattcg agcaagagtt
      481 cacgaattac ctgggtcggg aacactgcgt gggagtcgcc aacggcaccg atgccttgat
      541 gatcggcctg atcgcgcttg gagtcagacc tggcgatgag gtcgtggtac ccgccgtaac
      601 gtttttcgca accgcggaag cagtggcggc aattggagcc aagccagtgt ttgccgacgt
      661 cgagcttggt acctggacca tctcgaaacg aacggttgag ccgctactca ccgattccac
      721 agcagcgatc gtgccggttc acctcttcgg gaatccagcc ccaatgaacg agctcatgag
      781 cttggcggac gcaagcggag ttccggtact cgaagacgcc gcccaagccg ctggagcaac
      841 ttacgacgga aagatggccg gcgcgatcgg gtcagcggca tccttcagct tctatcccgg
      901 caagaacctc ggggcgatcg gagatgcggg ggcgctggtg acagacgatc ccgaggtcgc
      961 agacctcgcg cgaaagctcc gggaacacgg atcggcagac cggaaaatcc acaccgaagt
     1021 cggattcaac tccaggcttg actcaattca ggctgccgct ttgagcgtct ttctgcccca
     1081 actcgacgcg tggaccaagt caagacgtca agccgcagat gcctacggga actcgagcct
     1141 cgccggactg gtcgggcttc cggtcgaaac cgcgcaggcc cagtcttgcc accacctgta
     1201 cgtaataagc agtgatcgtc gtgacgagat acttatggcc cttagcgccg ctgagatcga
     1261 gtcgcgggtc tactacagtc cggcgatccc atttcagccc gcaatgaaag cattcgcccc
     1321 ggatcaccaa ctcccgggtg ctaccgcata cgaaaactcc gctctggctc tgccgatggg
     1381 cgaatcactc tcggtcgaag atgctcaaag agtcgccgct gtaatcagtt cggctctgag
     1441 ctgaccgggc gtctgcgttc gtcagcgcaa cccggccggc agctgctttg tgagggtgga
     1501 tctgacacct tcgttttcca acgagcgttc ggccatttcg atcagctcga ccacgtcgcg
     1561 cgcaattcgc gtgtacttct gtggggcacg gccagcgctg accgcatcca ggaacgcgtc
     1621 caattgggca ctgatcggtt ccgttgtcgg gagcttcggc gtgagaatgt caccggtgcg
     1681 gtaagaaagg tgatactggc cgaaagtttc cggatcctca aacacgaccc cgtggtcata
     1741 gacccggacc
//
LOCUS       DAOQMF010000465         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_49989_length_1748_cov_3.526285, whole genome shotgun sequence.
ACCESSION   DAOQMF010000465 DAOQMF010000000
VERSION     DAOQMF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49989_length_1748_cov_3.526285"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..343
                     /gene="acpP"
                     /locus_tag="PLV77_07875"
     CDS             98..343
                     /gene="acpP"
                     /locus_tag="PLV77_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC15802.1"
                     /translation="MNRDEVMDLVRNHLVTELELGPEQITETARFKEDFDADSLDLYE
                     LVMELEDQYGITVSEQEAAEIKTVGDALDFVVAKAVA"
     gene            318..1049
                     /locus_tag="PLV77_07880"
     CDS             318..1049
                     /locus_tag="PLV77_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNC15803.1"
                     /translation="MSWLKRSRERAGGHDPVTPDSGLASLIEGLPEDLKVPALTHSSW
                     TSERTDSYERLALLGDSVLGLAVADELYRTLPGLSAGALTKILNQTVSGTSCAEVGRD
                     LGIPDMLLAADPGEPDGKQTPARLLLEGERPLPEITEALIGACYLHYGFGATSSAVIE
                     AFRSRISLVRENQTDFKSALQEEAARHGETVSYEVIAAHGPAHQRTYEVAVIFREEEI
                     GRGEGRSKKAAGQAAAEVALERLGD"
     gene            1158..>1748
                     /locus_tag="PLV77_07885"
     CDS             1158..>1748
                     /locus_tag="PLV77_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC15804.1"
                     /translation="MYLRSVTMKGFKSFPEKTELAFSPGVSVIVGPNGSGKSNITDAV
                     LWCLGEQSPGAIRGTSMQDVISVGGKNVGARSEAEVEVVIDNAEGQLGDEFTEISITR
                     KLDRNGDGGYWMNGARCRLSDIIELLSDANLGREMHSVISQGRVDSIVHSKPRERRAL
                     IEEAAGLGKFRKRRRRSELKLLATRDNLDRALDVESA"
BASE COUNT          341 a          549 c          577 g          281 t
ORIGIN      
        1 ggtcgacgcc cccgggctga ccgaagagat gcttcaccgg gtcggcgtta accgcggctc
       61 gatcgattcc gcaactgacg tctgatagga aacgggaatg aaccgcgacg aagtaatgga
      121 tctggtccgc aaccacctgg tcaccgaact ggaactggga cccgaacaga tcaccgaaac
      181 ggcgaggttc aaggaagact tcgacgccga ttcactcgat ctctatgaac tcgtgatgga
      241 actcgaggac cagtacggga tcacggtctc cgaacaggag gccgcggaaa tcaagaccgt
      301 tggggacgcg cttgactttg tcgtggctaa agcggtcgcg tgagcgtgcc ggggggcacg
      361 acccggtaac cccggattcg ggcctggcga gcctgatcga aggcctgccg gaggacctca
      421 aggttccggc cctgacccat tcctcctgga ccagcgagcg gacagactca tacgaacggc
      481 ttgccctgct cggtgacagc gtcctcggac tggccgtcgc ggacgagctt taccgcaccc
      541 tccccgggct gagtgcgggg gcgctgacca agatcctcaa ccagacggtc agtggaacct
      601 cctgcgccga ggtcggtcgc gatctcggca tcccggacat gttgctggcc gcggacccgg
      661 gcgaacctga cggcaagcag accccggccc gcctgctgct ggagggcgag cggccgctgc
      721 cggaaatcac cgaagcattg atcggcgcct gttacctcca ctacggattc ggggccactt
      781 cgagtgccgt gatcgaagcc ttccggtccc ggatctccct ggtccgggag aatcagacgg
      841 atttcaagtc ggccctccag gaagaggccg cgcgccacgg tgaaaccgtc tcctacgaag
      901 tgattgcggc ccacggtccg gcccatcaga ggacctacga ggtagccgtc atcttccgtg
      961 aggaagagat cgggaggggc gaaggcagaa gcaagaaagc agccggtcag gcggccgccg
     1021 aagtcgccct cgaaaggctt ggcgactgaa actccctgca ctttgcggga aacggttttt
     1081 cccagtcggg ctgcggtgtt gaggcgttcc ggggtgggga atcgtccgtc cggcggaacc
     1141 ggcaatctgt aaggcgcatg tatctgcgct cagtgaccat gaagggcttc aagtccttcc
     1201 cggaaaagac cgagctggcc ttctcgcccg gggtcagcgt gatcgtcggg ccgaacggct
     1261 ccgggaagtc gaacatcacc gacgccgtgc tctggtgtct cggcgagcag agcccgggcg
     1321 cgattcgcgg cacctcgatg caggacgtga tttcggtcgg cggcaagaac gtcggcgccc
     1381 gctccgaggc cgaggtcgag gtcgtgatcg acaacgccga aggtcaactg ggggacgagt
     1441 tcacggaaat ctcgatcacc cgcaagctgg atcgaaacgg agacggcggc tactggatga
     1501 acggcgcccg gtgccggctg tcggacatca tcgaacttct ctccgacgcc aacctcgggc
     1561 gggagatgca ttcggtgatc agccaggggc gggttgattc gatcgtccac tccaagcccc
     1621 gcgagcgccg cgccttgatc gaagaggccg ccggactcgg caagttccgc aagcgccggc
     1681 gccgctcgga gctgaagctg ctcgccaccc gcgacaacct cgaccgcgct ctcgacgtcg
     1741 agagcgcg
//
LOCUS       DAOQMF010000466         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_50196_length_1744_cov_4.110716, whole genome shotgun sequence.
ACCESSION   DAOQMF010000466 DAOQMF010000000
VERSION     DAOQMF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50196_length_1744_cov_4.110716"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1579)
                     /gene="rlmD"
                     /locus_tag="PLV77_07890"
     CDS             complement(146..1579)
                     /gene="rlmD"
                     /locus_tag="PLV77_07890"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNC15805.1"
                     /translation="MSATQPSSPPTRLRRGETFEGEIETLAYGGRGITKPDGYVVFVN
                     GGLPGDRVRALITKSKRGYAEANALEIITPSPDRLPDTDLHQGEACPGASWQALSYEK
                     QLAFKEEHVADALARIGKLGDVNIDPIVEADEQWRYRNKVEYSFGEQDGELVLGFHPR
                     GRWDEVLDIEDCHLSSEATNRARNEVLAWARAEGLNAYDQHDHRGILRNLVVREGRRT
                     GQVQTRLVTSRANFPKPPVDLHTIIDGPSGSTSGPTGVLGEEVLRERIFDLDIELSHD
                     AFFQTNTEMAEKLYGLAAEYAGLTGNEKLFDLFCGVGTIGLSMARQAGQVWGLEIVPE
                     AIENAKRNAKANGITNARFVAANARTGMRPLIEEAGRPDVVTIDPPRAGLSAKIVRRL
                     IECEARRIVYISCNPTTLAPNAAQLVEAGYRLTRVRPVDMFPQTPHIECVAQFDLVGE
                     VKYVESPTDRKRRERDERRQAAKEAEG"
BASE COUNT          258 a          546 c          615 g          325 t
ORIGIN      
        1 agcgtttcga gccccttggc caggggatgg tcgttggccc agaccgaggc gtagccggcc
       61 tcctcgcagg cggcggccag gggggcgcag acctcggccg gaagtccggc ggcaactccg
      121 aaagcgcgct tctgttcggg acgggtcaac cttcagcctc cttcgcggcc tggcgccgct
      181 cgtctcgttc acgacgtttg cggtcggtgg gggattcgac gtacttcacc tcgccgacca
      241 gatcgaactg ggcgacgcac tcgatgtgcg gggtctgggg aaacatgtca accggcctca
      301 cccgggtcag cctataaccg gcctcgacca gctgggcggc gttcggggcc agcgtggtcg
      361 ggttgcagga gatgtaaacg atccggcggg cttcgcattc gatcaggcgg cgcacgatct
      421 tggccgagag cccggcccgg ggcggatcga tcgtcaccac atcgggccgg ccggcttcct
      481 cgatcagggg gcgcatcccg gtgcgggcgt tggcggcgac gaaacgggcg ttggttatgc
      541 cgtttgcctt cgcgttccgc ttcgcgttct cgatcgcctc gggaactatt tccaggcccc
      601 agacctgacc ggcctgccgg gccatgctga gcccgatcgt gccgactccg cagaagaggt
      661 cgaagagttt ctcgttgccg gtcagcccgg cgtactcggc ggcgaggccg tagagcttct
      721 cggccatttc ggtattggtc tggaagaagg cgtcgtggga gagctcgatg tcgaggtcga
      781 agatccgctc ccgcaggact tcctcgccga gtaccccggt gggtccggaa gtcgaaccgg
      841 agggaccgtc gatgatcgtg tgaaggtcga ctggcggctt ggggaagttg gcgcgcgagg
      901 tgaccagccg ggtctggacc tgcccggtcc ggcggccttc gcggacgacc aggttgcgca
      961 ggatgccgcg gtggtcatgc tggtcgtacg cgttgaggcc ttcggcccgc gcccaggcga
     1021 ggacttcatt gcgggcccgg ttggtcgcct cggaggagag gtgacagtcc tcgatgtcca
     1081 gcacctcgtc ccagcgacca cgggggtgaa agccgagcac cagctcgccg tcctgctcgc
     1141 caaatgagta ttcgaccttg ttgcggtatc gccactgctc gtcggcctcg acgatcgggt
     1201 cgatgttgac gtctccgagc ttgccgatcc gggccagggc gtcggcgacg tgctcctcct
     1261 tgaacgcgag ctgcttctcg taactcagcg cttgccacga ggcgccgggg caggcttcac
     1321 cctggtggag gtcggtgtcg gggagccggt cgggactcgg ggtgatgatt tccagggcgt
     1381 tggcttcggc gtagccccgt ttcgacttgg tgatcaatgc ccgcacccgg tcaccgggca
     1441 gcccgccgtt gacgaacacg acataaccat ccggtttggt gatgccgcgg ccgccgtagg
     1501 cgagggtttc gatctcgcct tcgaaggttt cgccgcggcg cagtcgggtg ggagggctgg
     1561 agggctgtgt tgcagacatt ggacccacca ctatctcagg ctgaaagcgg ccttagtatt
     1621 cccctcttga ctgaaccgcg tgccaggcaa tacatcgacg gccacgagat ctccggcggc
     1681 cacctcgtca acctgatcga tcgggccgag gagctgaggg ccggccgccc cgacgagggg
     1741 ggct
//
LOCUS       DAOQMF010000467         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_50656_length_1736_cov_4.713861, whole genome shotgun sequence.
ACCESSION   DAOQMF010000467 DAOQMF010000000
VERSION     DAOQMF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50656_length_1736_cov_4.713861"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLV77_07895"
     CDS             <1..640
                     /locus_tag="PLV77_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNC15806.1"
                     /translation="VVHWLTHDSIGLGEDGPTHQPVEQLASLRAMPNLDVIRPADAAE
                     VAEAWKVIMPRIDHPTALVLTRQKVPVIDRSKFADASGLAKGGYVIADCDGEPELILI
                     ATGSEVHIALEAYETLTIEGVKVRVVSLPSWHIFENQDEAYRDSVLPPSVTRRISIEQ
                     ASTFGWERYIGEEGVAIGMTTFGASAPFKDLQKYFGFTPDRVVEEAKNLLGS"
     gene            645..>1736
                     /gene="pgi"
                     /locus_tag="PLV77_07900"
     CDS             645..>1736
                     /gene="pgi"
                     /locus_tag="PLV77_07900"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNC15807.1"
                     /translation="MSELRESEAFKTLESHRNDIGDRHLRDLFAEDPSRGTDLVAEAC
                     GLYFDYSKNRITRETIGLLAALARDRRMPERRDAMFRGEHINVSEDRAVLHVALRMPR
                     SRSLVVDGVDVVREVHEVLDRMAAFSESVRSGEWKGQTGKPIRRIINIGIGGSDLGPV
                     MAYEALKDYSRRDLEFRFVSNVDGTDFYEATLDADPAETLFIVSSKTFTTLETMTNAH
                     TARQWLLDGLGGDESAIARHFVAVSTNAEGVSEFGIDTDNMFGFWEWVGGRYSMDSAI
                     GLSTMLAIGPDRFSEMLDGFHAMDEHFLETNLETSLPALHGLLTVWYRNFFDAETHAV
                     FPYSEYLHRFPAYLQQLTMESNGKGVTLAG"
BASE COUNT          306 a          592 c          541 g          297 t
ORIGIN      
        1 ggtcgtccac tggctgactc acgattcgat cggcctcggc gaggacggcc cgacccacca
       61 gccggtcgag cagctcgcct cgctccgggc gatgccgaac ctggacgtga tccgcccggc
      121 cgacgcggcc gaggtcgccg aagcctggaa ggtgatcatg ccgcgcattg atcaccccac
      181 cgccctggtc ctgacccgcc agaaggtgcc ggtgatcgac cgcagcaagt tcgccgacgc
      241 ttccgggctg gcgaagggcg ggtacgtgat cgccgactgt gacggcgagc cggagctgat
      301 cctgatcgcg accggctccg aggtccacat cgcactcgag gcatacgaaa ccctgaccat
      361 cgagggcgtc aaggtccggg tcgtttcgct gccttcctgg cacatcttcg aaaaccagga
      421 cgaggcgtac cgggattcgg tccttccgcc ttcggtcacc cggcggatct cgatcgaaca
      481 ggcttcgacc ttcggctggg agcgctacat cggcgaggaa ggcgtggcga tcggcatgac
      541 caccttcggc gcatcggccc ccttcaagga cctccagaaa tacttcggct tcacccccga
      601 ccgggtggtc gaagaagcga agaacctgct cgggagctga accggtgagc gagctccggg
      661 agtcagaagc cttcaagacc ctcgagagcc accgcaacga cataggcgac cgtcacctga
      721 gggacctctt cgccgaggat ccctcgcgcg ggaccgatct ggtcgccgag gcctgcggtc
      781 tctacttcga ctactcgaag aaccggatca cccgtgagac gatcggcctg ctcgctgccc
      841 tcgcccggga ccgccgcatg ccggagcgtc gcgacgcgat gttccggggc gagcacatca
      901 acgtctccga ggaccgggcg gtgctgcatg tggcgctgcg gatgccgcgc agccgctcgc
      961 tggtcgtcga cggggtcgac gtggtgcggg aagtccacga ggtcctcgac cgcatggccg
     1021 ccttctccga gtcggttcgt tcgggcgaat ggaagggcca gaccggcaaa ccgatccggc
     1081 ggatcatcaa catcggcatc ggcggatctg acctcggccc ggtgatggca tacgaggcgc
     1141 tcaaggacta ttcccgccgt gacctcgagt tccgtttcgt ttctaacgtg gacggcactg
     1201 acttctacga ggcgaccctt gacgccgacc cggccgagac gctcttcatc gtttcctcga
     1261 aaaccttcac gaccttggag acgatgacca acgcccacac cgcccgccag tggctgctcg
     1321 acggcctcgg cggtgacgag tcggccattg ccaggcactt cgtcgcggtc tcgaccaacg
     1381 ccgaaggagt ctcggagttc gggatcgaca ccgacaacat gttcggattc tgggaatggg
     1441 tcgggggccg ctactcgatg gattcggcga tcggcctctc gaccatgctg gcgatcggcc
     1501 ccgatcgttt cagcgagatg ctcgacggtt tccacgcgat ggacgagcac ttcctcgaaa
     1561 cgaatctcga gaccagcctg ccggcccttc acggcctgct caccgtctgg taccggaact
     1621 tcttcgatgc cgagacccac gccgtcttcc cctacagcga gtatctgcac cgcttccccg
     1681 cctacctgca gcagctgacc atggagtcga acggcaaggg cgtgactctc gccggg
//
LOCUS       DAOQMF010000468         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_50905_length_1732_cov_2.985092, whole genome shotgun sequence.
ACCESSION   DAOQMF010000468 DAOQMF010000000
VERSION     DAOQMF010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50905_length_1732_cov_2.985092"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLV77_07905"
     CDS             <1..191
                     /locus_tag="PLV77_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15808.1"
                     /translation="GEARKRAEIQVATGPAASAVSTSGTSEAEAREAAVNWLRGQISA
                     LQQEISKSGDSGKDSGSN"
     gene            191..478
                     /locus_tag="PLV77_07910"
     CDS             191..478
                     /locus_tag="PLV77_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15809.1"
                     /translation="MALFSRGGQTGRERRAMQDHLRELDEMRRSNLGELGQMTVEMAS
                     VGSFDRRRLSEQAAEVAKIDREADLIARGLEEGLTLQELEDLARGDASTSR"
     gene            503..>1732
                     /locus_tag="PLV77_07915"
     CDS             503..>1732
                     /locus_tag="PLV77_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC15810.1"
                     /translation="MPDSSEERDLGTFDLTVVGGGAAGLWAALCAAEAGGSVCLVSRK
                     PLAESASFHAQGGLAAALDPADSPDQHFEDTMKAGRSLCRSSAVRALVDEGPGIVDDL
                     VRRGMNFDREPEGTLSLALEGGHSHRRIIHSGGSQTGREITSALAQMVADSDRIEVLE
                     ETSAVALISDGNRCYGVITEDGTIRSAATLMATGGAAALWRRTSNPWGAIGAGPVLAS
                     AAGAELADLEFCQFHPTCLSAPGTVFDGALITEAIRGEGATLLDLDGERFTDELAPRD
                     DVTAAILAQTTKQEGEPVRLDLTCVDPARFPSVFQLLETAGFDATTEPVPVSPGAHYM
                     MGGIAVDLDGRTSIPGLYAAGECACTGLHGANRLASNSLTECFVFGRRAAVAGLQEVA
                     RVMPPPRDWRFDPPQPPT"
BASE COUNT          287 a          598 c          579 g          268 t
ORIGIN      
        1 ctggtgaagc ccggaagcgg gccgagattc aggtcgccac cggcccagca gcaagtgccg
       61 tcagtaccag cggaacttcc gaggcggagg caagagaggc cgccgtgaac tggctgcgtg
      121 gccagatttc agctctccaa caggaaattt ccaagtccgg cgattccggg aaggattcgg
      181 gaagtaacta atggctcttt tcagtcgtgg tggccagacc ggacgcgagc gacgcgcgat
      241 gcaggaccat ctgcgcgaac tcgacgagat gcgccgcagc aatctgggcg agctcgggca
      301 gatgaccgtc gagatggcgt cagtgggcag cttcgaccgg cgccggcttt ccgagcaggc
      361 agcggaagtg gcgaagatcg atcgcgaggc cgatctgatc gcccgcggtc tcgaggaggg
      421 cctgaccctc caggagctgg aagacctggc tcgcggcgac gcgtccactt cccgctagat
      481 gcgaacccga cggaactgac ggttgccgga tagctccgaa gagcgggatc tcgggacctt
      541 cgacctgacc gtcgtcggcg gtggcgcggc cgggctctgg gcggcactct gcgccgccga
      601 ggcgggcggt tcggtctgcc tggtctcccg caagccgctg gccgagagcg ccagcttcca
      661 cgcccaaggc gggctcgccg cagcactcga cccggccgac tcgccggatc agcatttcga
      721 ggacacgatg aaggccggac ggagtctctg ccggtcatcg gccgtgcggg cgctggtcga
      781 tgagggtccg ggcatcgtcg atgatctggt ccggcgcggc atgaactttg accgcgagcc
      841 tgaaggaacc ctctcgctcg ccctggaggg cggccacagc caccgccgga tcatccactc
      901 cggcggcagc cagaccggtc gcgaaatcac ctccgccctg gcccagatgg tcgccgacag
      961 cgaccggatc gaggtcctgg aagagacctc ggcggttgcc ctgatcagtg acggcaaccg
     1021 ctgctacgga gtgatcaccg aagacggcac gatccggagt gccgcaaccc tgatggcaac
     1081 cggtggtgcc gccgccctct ggcgccggac ctccaacccc tggggagcga ttggagccgg
     1141 cccggtgctg gccagtgcgg ccggggccga acttgccgac ctcgagttct gccagttcca
     1201 cccgacctgc ctttcggccc ccggcaccgt ctttgacggc gccctgatca ccgaggcgat
     1261 ccgcggtgaa ggggccaccc tgctcgacct cgacggcgag cgcttcaccg acgagctcgc
     1321 accccgggac gatgtgacgg ccgcgatcct cgcccagacc acaaagcagg aaggcgaacc
     1381 ggtccggctc gatctgacct gcgtcgatcc ggcccgtttc ccgagcgtct tccagttgct
     1441 tgaaaccgcc ggcttcgacg ccaccaccga gccggtgccc gtttctcccg gcgcccatta
     1501 catgatgggc gggatcgctg tggaccttga cggcaggacc tcgatccctg gcctttacgc
     1561 ggccggtgaa tgcgcctgca ccgggctcca cggagccaac cgcctcgcct ccaactcgct
     1621 gaccgaatgt ttcgtcttcg gccgccgcgc cgcggttgcc ggacttcagg aagtggccag
     1681 ggtgatgccg ccgccgcgcg attggcgttt tgacccgccg cagccgccga cc
//
LOCUS       DAOQMF010000469         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_51077_length_1729_cov_3.488053, whole genome shotgun sequence.
ACCESSION   DAOQMF010000469 DAOQMF010000000
VERSION     DAOQMF010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51077_length_1729_cov_3.488053"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..694
                     /locus_tag="PLV77_07920"
     CDS             146..694
                     /locus_tag="PLV77_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC15811.1"
                     /translation="MAHAEELLRREEAVGELARSPATPERVLEAVSLLSAATGVEGMV
                     GGQYIDVSAQALDADGLRHLHSLKTGRLIRASIEVALALGGVTEASREPFRRFATQIG
                     LLFQIVDDILDVTGSDEELGKPQGSDERHGKVTYVSLYGLDRARSLAAETHEGALAAL
                     AGAGGETEDLKRIADYIYSREQ"
     gene            691..>1729
                     /locus_tag="PLV77_07925"
     CDS             691..>1729
                     /locus_tag="PLV77_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNC15812.1"
                     /translation="MSEETKDTKPLLPGIASPADLKGLSDEQLQRVADETRSYIIDVI
                     GEIGGHFGANLGTCEIAVALHSLLESPRDKILWDVGHQAYPHKVLTGRRDQLSTIRQY
                     EGLAPFCSIPESEHDIMGAGHASTSISYGVGLKEAMRKGIGEDGKVVAVIGDGALTGG
                     VAYEAMHAAGGYQTPMVILLNDNGMSISPNVGALSSYFQRARLKPGLFHAREEVEDRL
                     TRLPLGLGKRIERLGPELKSAIKAYWAPGLLFEELDLAYMGVIDGHDVGAMRRALTEA
                     FAADRPVVVHVHTVKGRGFAPAEQGGQAGQEKWHAAKPGSIVDGAPADSKPKLVPVQE
                     SDAPASIAPEIL"
BASE COUNT          317 a          562 c          592 g          258 t
ORIGIN      
        1 cgcggtcggg caggtggtgg aacgagatcg agttggtcag cgcgagcgtc ggcgcgtaga
       61 cgaggccgta cgccgccgag agcgcgagga acgggccgac gctggtctgg ctcgagagga
      121 gccagacgag gagcgcgccg agcaggtggc tcacgccgag gaacttctcc gtcgagaaga
      181 agcggtcggc gaactggccc gctctccggc aaccccggaa cgggttcttg aagcggtcag
      241 cctgctcagc gccgcgaccg gggtggaagg aatggtcggt ggccagtaca tcgacgtctc
      301 ggcccaggcc ctggacgcgg acgggctcag gcacctccat tcgctgaaga ccggccggct
      361 catccgggcc agcatcgaag tggcgctggc gctcggcggg gtcacggaag cgagccgtga
      421 gccgttccgg cgcttcgcca cccagatcgg cctgctcttc cagatcgtcg acgacatcct
      481 cgacgtgacc ggaagcgacg aagaactcgg gaagccccag ggcagcgacg agcgtcacgg
      541 aaaggtgact tatgtcagcc tgtatggatt ggaccgggca cggtcgctgg ccgcggagac
      601 ccacgagggg gccctggccg cgctggccgg tgccggcggc gaaaccgaag acctgaaacg
      661 catcgccgac tacatttatt cgagagaaca atgagcgaag agaccaaaga cacgaagcct
      721 ctgcttcccg ggatcgcctc tccggccgac ctcaagggtc tcagcgacga gcagctccag
      781 cgggtcgccg acgagacccg ctcctacatc atcgatgtga tcggtgaaat cggcggtcac
      841 ttcggggcca acctgggaac ctgtgagatt gcggtcgccc tgcacagcct gctcgaatcg
      901 cccagggaca agatcctctg ggacgtcggc catcaggcct atccgcacaa ggtgctgacc
      961 ggccggcgcg accagctctc gacgatccgc cagtacgaag gcctcgctcc gttctgctcg
     1021 attccggaat ccgagcacga catcatgggt gccggtcacg cttcgacctc gatctcctac
     1081 ggggtcggcc tcaaggaagc gatgcgaaag gggatcggcg aggacggcaa ggtcgtcgcg
     1141 gtgatcggtg acggcgccct gaccggcggc gtcgcctacg aggcgatgca tgctgcgggc
     1201 ggctaccaga ccccgatggt gattctgctc aacgacaatg gcatgtcgat ctcgccaaac
     1261 gtcggggcgc tttccagcta cttccagagg gcccggctga agcccgggct cttccatgcc
     1321 cgcgaagagg tcgaggatcg cctgaccagg ctgccgctcg gcctcggcaa gcggatcgaa
     1381 aggctcgggc cggaactcaa gtcggcgatc aaggcctact gggcccccgg cctgctcttc
     1441 gaagaactcg atctcgccta catgggtgtg atcgacggcc acgacgtggg ggcgatgcgc
     1501 cgggctctga ccgaagcttt cgccgcggac cggccggtgg tcgtccacgt ccacacggtc
     1561 aagggccgcg gtttcgcccc ggccgagcag ggcggccagg ccggccagga gaaatggcat
     1621 gcggccaagc ccggctctat cgttgacggg gctcccgccg actcgaagcc gaagctggtc
     1681 ccggtgcagg aatccgatgc cccggcctcg attgctcccg agatcctcg
//
LOCUS       DAOQMF010000470         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_51302_length_1725_cov_3.362874, whole genome shotgun sequence.
ACCESSION   DAOQMF010000470 DAOQMF010000000
VERSION     DAOQMF010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51302_length_1725_cov_3.362874"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="PLV77_07930"
     CDS             complement(<1..1450)
                     /locus_tag="PLV77_07930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNC15813.1"
                     /translation="MVGRSQSLEAIAGSHFEVVVIGGGITGAGVALDAASRGYSVALL
                     EGRDYAIGTSSRSSKMVHGGLRYLENFDVGLVREALLERQLLVELAPHLVYPTPFLVP
                     SFGEEKPDRKVGLGLGAYDVLAGAPSGRNRRERRWRRRPAEAEESWSPDRHRTIDREE
                     LLELVPALETRDPESAYLFYDCQTDDARLVLTVLGEAERFGAVCLNGANVTRVLEKSG
                     KARQVEFTEIASGETVVVEADNIVNATGVWADQIRPGEIEREEDIPKIAPSRGAHLLF
                     SADDVDMGRAACIVPAGEGRRIFALPWYGRTLVGTTDVDHDGGIEEVAATEDDIEYLM
                     NALNEFMDLSLTDSDIVGAFAGVRPLISTGDPRKSVDISRQAELYETSSGMLTITGGK
                     LTTWRRMAKQTVDRIVERAGREARCQTAEIPLGMEIDPAELSAPEGVSKESLAQLAFR
                     YGHAARGVIRIAESDSEMAKPIVDGMPDLMAEA"
     gene            complement(1444..>1725)
                     /locus_tag="PLV77_07935"
     CDS             complement(1444..>1725)
                     /locus_tag="PLV77_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15814.1"
                     /translation="SASGSGSGSSGSGNGNGSGSGSGTNGGDGSSSATTLPPTDTVAP
                     VIPEAPSAEVLAASEAGPQPSPLVATLVGLAVSGFLCGGTILLVRRLNW"
BASE COUNT          255 a          594 c          584 g          292 t
ORIGIN      
        1 gggcttcagc catcaggtcc ggcatcccgt cgacgatcgg tttggccatt tcggaatccg
       61 attcggcgat gcggatcact cccctggcgg cgtggccgta acggaaggcg agctgggcca
      121 gcgactcctt gctgacgcct tccggggcgc tcagctcggc cgggtcgatc tccatcccga
      181 gcgggatctc ggccgtctgg cagcgcgctt cccggcctgc cctttcgacg atccggtcca
      241 cggtctgctt cgccatccgc cgccaggtgg tgagcttgcc accggtgatg gtcagcatcc
      301 ccgatgaggt ctcgtagagc tcggcctggc gggagatgtc gacggatttg cggggatcac
      361 ccgtgctgat caggggcctg accccggcga aggccccgac gatgtcggag tcggtcaggg
      421 aaaggtccat gaactcgttc agcgcgttca tcaggtactc gatgtcgtct tcggtggccg
      481 cgacttcctc gatgccgccg tcgtgatcga cgtcggtggt cccgaccagg gtccggccgt
      541 accagggcaa ggcgaagatc ctgcggccct cgccggccgg cacgatgcag gcggcccggc
      601 ccatgtctac gtcatcggcg gagaaaagca ggtgggcgcc ccggctcggt gcgatcttcg
      661 ggatgtcctc ttcccgttcg atttcgcccg gccggatctg gtcggcccag accccggtcg
      721 cgttgacgat gttgtcggcc tcgacgacca cagtttcgcc cgatgcaatt tcggtgaact
      781 cgacctgccg ggccttgccg ctcttctcca gcacccgggt gacgttggcg ccgttcaggc
      841 agaccgcgcc gaagcgttcg gcctcgccca gcacggtgag gacgagccgc gcgtcatcgg
      901 tctggcagtc atagaagagg taggccgatt cagggtcgcg cgtctccagg gccgggacca
      961 gttcgagcag ctcttcccgg tcgatcgtcc ggtgccggtc cggtgaccag gattcctccg
     1021 cctcggccgg tcgccggcgc cagcgccgct cgcggcggtt gcgcccgctg ggcgccccgg
     1081 ccagcacgtc gtaggccccg aggcccagcc cgaccttgcg atccggcttc tcctccccga
     1141 agctcggcac cagaaacggg gtcggataga ccagatgcgg agccagctcg accaggagct
     1201 ggcgctcgag cagcgcttca cggaccagcc ccacgtcaaa gttctcgagg taccggaggc
     1261 cgccgtgaac cattttcgag gagcgggatg aggttccgat cgcgtagtcg cgaccttcca
     1321 gcaaggcgac cgagtagccg cgggaggccg cgtcgagcgc gactccggcc ccggtgatcc
     1381 cgccgccgat cacgaccacc tcgaaatgcg atccggcgat cgcctccagc gactggcttc
     1441 gacctaccaa ttgagccgcc ggaccagcag gatggtgccg ccgcagagga aaccgctgac
     1501 cgcaagcccg accagggtcg caactagcgg tgaaggctga gggccggcct cggatgcggc
     1561 cagcacttcg gcgctcggcg cttccggaat gaccggtgcc acggtgtcgg tcgggggcag
     1621 cgtggtggca gaagatgatc cgtcgccgcc attggtgccg ctgccgctgc cggagccgtt
     1681 gccgttcccg gatccgctgg atccggagcc ggatccgctc gccga
//
LOCUS       DAOQMF010000471         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_51321_length_1725_cov_2.682036, whole genome shotgun sequence.
ACCESSION   DAOQMF010000471 DAOQMF010000000
VERSION     DAOQMF010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51321_length_1725_cov_2.682036"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1147
                     /locus_tag="PLV77_07940"
     CDS             230..1147
                     /locus_tag="PLV77_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNC15815.1"
                     /translation="MSPGHPSGRQFEITHGSQSATVVEVGGGIRTYEVEGRPVLDPYP
                     VERICDGAHGAPLIPWPNRLDRGRYSFDGEAYQLPLTEPEQDNAIHGFLRWVTWAAEV
                     HGPDAVTMSTTVAPRPGYPFWLDVSVCYRLGDKGLEVSTEIVNKGERACPVGHGQHPY
                     LSAGSGLIDDCLLQHPGTVRILTDDRQLPAGREEASGTKFDFAEPRQLGDIEIDFAFT
                     DLRRDGEGKAWTRLEGADGKTASIWVDRNYPLVELYTGHTLAPDRARTGLGTEPMTCA
                     PDAFNNGEGLIRLEPGESASAVWGACLEG"
     gene            1150..>1725
                     /locus_tag="PLV77_07945"
     CDS             1150..>1725
                     /locus_tag="PLV77_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15816.1"
                     /translation="MAHRQTFLILGASGDLTSRLLLPGLASLVDAGQTGDLLLVGSSV
                     DEWSQEEWRDRVRNSLEKSGGAGEGSMALAASARYVQADVTDVEGVRSVLAAVEGQPI
                     IYFALPPEVSITACEVLRKVGCPPETRLVLEKPFGRDAESAEAFNRLLAEIVPESQVH
                     RVDHFLGLSTVLNIFGLRFANRVFEPMMNSHH"
BASE COUNT          283 a          572 c          571 g          299 t
ORIGIN      
        1 gaatctgtcg tctgcggccc ggatcccgaa ccgatcgtcg aggagatagg ccggtatctg
       61 gacgccggct tcagccatgt ctatgtccac caggtcggcc ccgacactcc gggtttcttc
      121 gacttctttc gcgaccgggt actgccggag ttccaggcct gagtccggtc cggacctcac
      181 cccgccgtaa cggcccgtcg cggaagccgc ggtcgggtac ttttggcaca tgagccccgg
      241 tcacccttcc ggacgacagt tcgaaatcac gcatgggtcg cagtcagcga cggtcgtcga
      301 agtcggcggt ggcatccgga cctacgaggt cgagggacga cccgtgctcg acccctatcc
      361 ggtcgaacgg atctgcgacg gggcccacgg ggctcccctg atcccgtggc ccaaccggct
      421 cgaccgcggc cgctacagct tcgacggcga ggcatatcaa ctgcctttga ccgaaccgga
      481 acaggacaac gcgatccacg gcttcctgcg ctgggtcacc tgggcggcgg aggttcacgg
      541 acccgacgca gtgacgatgt ccaccacggt cgcgccccgg ccgggttatc cgttctggct
      601 cgatgtttcg gtctgctacc ggcttggcga caagggactc gaagtgtcaa ccgaaatcgt
      661 caacaagggc gagcgcgcct gcccggtcgg ccacggccag catccctacc tgtcggccgg
      721 cagcggcttg atcgacgact gcctgctcca gcatcccggc accgtccgga tcctcaccga
      781 tgaccgccag ttgccggccg ggagggagga ggcgtcgggc acgaaattcg acttcgccga
      841 gccgcgtcag ctcggggaca tcgagatcga cttcgcgttc accgacctgc gtcgcgacgg
      901 ggaggggaag gcctggaccc ggctcgaggg tgccgacggg aagactgcgt cgatctgggt
      961 cgaccggaac tacccgctgg tggagctcta cacgggccac acgctcgccc cggaccgggc
     1021 ccgtaccggc ctcgggaccg agccgatgac ttgtgctccg gatgccttca acaatggcga
     1081 ggggttgatc cggctcgagc ccggggagag cgcgtcagcc gtctggggcg cttgcctcga
     1141 gggctgaaca tggcgcaccg gcagacattc ctgatccttg gcgccagcgg tgacctgact
     1201 tcccggctgc ttttgccagg gctcgcctcg ctcgtcgatg ccggccagac tggcgacctg
     1261 ctcctggtcg gcagcagcgt ggacgagtgg agccaggaag agtggcggga ccgggtccga
     1321 aactcgctcg agaagtcggg aggagcgggg gaggggtcca tggcactcgc cgcctccgcc
     1381 cgctacgtcc aggccgacgt caccgacgtg gaaggcgtga gaagcgttct ggccgcggtc
     1441 gaaggccagc cgatcatcta tttcgcgttg ccgcccgagg tgtcgatcac cgcctgtgag
     1501 gtactgcgga aggtcggctg tccacctgaa acccggctcg ttctcgaaaa gccgtttggc
     1561 cgggacgcgg aatcggctga agccttcaac cgtttgctgg cggagatagt tccggagagc
     1621 caggttcacc gcgttgacca ctttctcggc ctctccacgg tgctgaacat cttcggtctc
     1681 cggtttgcga accgggtctt cgagccgatg atgaactccc atcac
//
LOCUS       DAOQMF010000472         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_51408_length_1723_cov_3.300360, whole genome shotgun sequence.
ACCESSION   DAOQMF010000472 DAOQMF010000000
VERSION     DAOQMF010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51408_length_1723_cov_3.300360"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLV77_07950"
     CDS             <1..915
                     /locus_tag="PLV77_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNC15817.1"
                     /translation="GRVIDGGLEEVRIEEQLPRGRHIRREGEDRAQGDQVSPAGIELS
                     PESLAAMASAGIGEVIVARRPRVAVISTGDELVAPGEPVLRGRIPDSNGLLVTMLAAD
                     AGALAEADHAGDGPGQLEAALGRNGGCDALVLTGGVSVGAFDPVKALFEGDESVRFDR
                     VAMQPGKPQAFGRLGESGPLVFGLPGNPVSAWVSFQMFVRPALRKMQGAARLFPEAVP
                     ARATEDWKTPQGRMQVIPVRISDENERKVLPAATGGSGSHLIASLAAADGYALVEPEV
                     EKIKAGDLVPTVRLRDPEQFNHPQRRQP"
     gene            912..1382
                     /gene="moaC"
                     /locus_tag="PLV77_07955"
     CDS             912..1382
                     /gene="moaC"
                     /locus_tag="PLV77_07955"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNC15818.1"
                     /translation="MTFTHLDESGQARMVDVTAKQPTVRSATASGFVRCPPAVIAALR
                     DEDLPKGDALAVARVAGIQGAKKCAELLPLAHVIGVHGAAVSFETGDEGVSVSATVRT
                     ADRTGVEMEALTAVSVAALTLIDMFKGIDKGVTIESVRLEEKTGGKSGTWRRQA"
     gene            1382..>1723
                     /locus_tag="PLV77_07960"
     CDS             1382..>1723
                     /locus_tag="PLV77_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNC15819.1"
                     /translation="MPEPATAVVLAGGRGSRLGGAEKGEIRLDGRRLIDIVVAAARES
                     GCERIVIAGDIESEGAITIREDPPFGGPAAGLAAALPAIEDDWILLLACDLPHARLLI
                     DLLTGAFREVGP"
BASE COUNT          269 a          581 c          617 g          256 t
ORIGIN      
        1 ggccgcgtga tcgacggcgg cctcgaggag gtccggatcg aggaacagct gcccaggggc
       61 cgccacatcc gccgggaggg cgaggaccgg gcccagggtg atcaggtctc accggccgga
      121 atcgagcttt cgccggaatc tctggccgcc atggcctcgg ctggcatcgg cgaggtgatc
      181 gtcgcccgca ggccacgcgt ggcggtgatc agcacgggcg atgaacttgt cgctcccggc
      241 gaaccggttt tgcgcggccg gatcccggac tcgaatggcc tgcttgtgac catgctggct
      301 gccgacgccg gtgccctggc cgaggccgac cacgccggcg acgggcccgg tcaactcgag
      361 gcggcgcttg gccggaacgg tggctgcgac gcattggtcc tgaccggcgg ggtcagtgtc
      421 ggagccttcg acccggtcaa ggcactcttc gaaggagacg aatcggtccg cttcgaccgg
      481 gttgcgatgc agcccggcaa accgcaggcc ttcggccgac tgggtgaaag cggaccgctc
      541 gtcttcgggc tgcccggcaa cccggtcagc gcctgggttt cctttcagat gttcgtccgt
      601 cccgccctgc gaaagatgca gggggcggcc cggctctttc ccgaggcggt cccggcccgg
      661 gcaacggagg actggaagac cccgcaaggc cggatgcagg tgattccggt gcggatctcc
      721 gacgagaacg agcgaaaggt gctgccggct gcgaccggtg gctccggctc ccacctgatc
      781 gccagcctgg cggcggccga cggttacgcc ctggtcgagc ccgaggtcga gaagatcaag
      841 gccggcgatc tcgtaccgac cgtccgcctg cgtgacccgg agcagttcaa ccacccccag
      901 aggagacaac cttgaccttc acccacctag acgaaagcgg ccaggcccgg atggtcgacg
      961 tgaccgccaa gcagcctacc gtgcggtcgg ccacggcttc aggtttcgtg cgctgcccgc
     1021 cagcggtgat tgcggccctg cgcgatgagg acttgccgaa gggtgatgcg ctggccgtgg
     1081 cccgggtggc cggtatccag ggggccaaga aatgcgccga gctcctgccg ctggcccacg
     1141 tgatcggcgt gcacggtgcc gcggtgtcat tcgagaccgg ggacgaaggg gtgagcgtca
     1201 gcgcgacggt gcgcaccgcc gaccggaccg gagtcgagat ggaggccctg accgcggtct
     1261 cggttgccgc cctcaccctg atcgacatgt tcaaggggat cgacaagggc gtgacgatcg
     1321 aatcggttcg cctcgaagag aagaccggcg gcaaatccgg cacctggcgg aggcaggcct
     1381 gattcccgaa cccgccactg cggtcgtcct cgccggcggc cggggctccc ggctcggcgg
     1441 cgccgagaag ggcgaaatca gactggacgg ccggcgcctg atcgacatcg tcgtggctgc
     1501 cgcccgcgaa agcggctgcg aaaggatcgt catcgccggc gacatcgaat ccgaaggggc
     1561 gatcacgatc cgtgaggatc cgcccttcgg cgggccggcg gccgggctcg cggctgcctt
     1621 gccggcaatc gaggacgact ggatcctcct gctggcctgc gacctccctc atgcccggct
     1681 tctgatcgac ctgctgaccg gcgccttccg cgaggtcggc ccg
//
LOCUS       DAOQMF010000473         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_51773_length_1717_cov_3.040915, whole genome shotgun sequence.
ACCESSION   DAOQMF010000473 DAOQMF010000000
VERSION     DAOQMF010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51773_length_1717_cov_3.040915"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLV77_07965"
     CDS             complement(<1..528)
                     /locus_tag="PLV77_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15820.1"
                     /translation="MRRYLVLLAALLAGMFIVGGVADAAGKQVKPARPSVKILTPASS
                     QLAKGTVRIQVKANKGAKVRLSAIARSFDTPNQRLFTNRTLYLRKKAGMAISMSLTPE
                     AKATARTCQALTLIITAKNGKKTTRVTRSVNRDLGDCQLSPVDLSRAADCDFIAEPKE
                     GMCMLPFPDDYYTVED"
     gene            700..>1717
                     /locus_tag="PLV77_07970"
     CDS             700..>1717
                     /locus_tag="PLV77_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNC15821.1"
                     /translation="MTSTFRRRFGLMLLLLPVLFTLSQPDPGKANAGLTIPSTTTRAA
                     TAPFNKAAMWIWYIDDSNGGNIGSIIRQARRAHIGTLYIKSGDGVDAWSQFNRPVVRR
                     FHRAGLKVCGWQYIYGRRPIAEAKISAVAKRRGADCFVIDAESEFEGAYAAADWYIRS
                     LRQRVGRRFPIGLAGFPYANYHPGYPYSVFLGPGAANANVPQVYWHTIGDSVRTALEI
                     TWEQNSIYKRPIFPVGQTYEDPGARELLAYRRFMLNYGTRPSWWSWQETSPAEWKILA
                     RPVSRVSGYRAYSGHVTLEKGDRGDQVLWLQQHLIAAGYSMEPTGIFSRSTYKAVRRF
                     QARRG"
BASE COUNT          289 a          560 c          544 g          324 t
ORIGIN      
        1 gtcctcgacc gtgtagtaat cgtcggggaa gggcagcatg cacatgcctt ccttcggttc
       61 ggcgatgaag tcgcagtcgg cagcccggct cagatcgacc gggctgagct gacagtcgcc
      121 gagatcccgg ttgaccgacc tcgtgaccct ggtcgtcttc ttcccgttct tcgcagtgat
      181 gatcagggtc agggcctgac aggtccgcgc ggtggccttt gcctccggag tcaggctcat
      241 gctgatcgcc atgcccgcct tcttgcgcag gtagagcgtg cggttcgtga agagacgctg
      301 gttgggcgtg tcgaaggatc tggcgattgc cgagaggcgc actttcgccc ccttgttcgc
      361 tttgacctga atcctgacgg tgcccttggc gagctggctt gacgccgggg tcaggatctt
      421 gaccgaaggc ctggctggct tcacctgctt gccggcagcg tctgcaactc cgccgacaat
      481 gaacattccg gcaagcagag ccgccagcag aacgaggtaa cgacgcatcc tttgattcct
      541 cccaaacaga gtttgaactt ctccctgccc cgcaggctac agggcaggga ggtgaaatga
      601 aagaaagctt gcttgcgggt ccgaggccgc cgcttgccgg gggttctcct gcgaggctga
      661 atccgctccg atcagccatc aaccgtgttc cagaccctga tgacatcgac tttccgccgg
      721 cgcttcgggc tgatgctcct gctgctgccg gtcctgttca cgctttccca gccggatccc
      781 ggcaaggcca acgccggcct cacgatcccg tccacgacga cgagggcggc gaccgctccc
      841 ttcaacaagg cagcgatgtg gatctggtac atcgacgatt ccaacggtgg caacatcggt
      901 tcgatcatcc gccaggcccg gcgggcccac atcggcacgc tctacattaa gtccggtgac
      961 ggcgtcgacg cctggagcca gttcaaccgg cccgtggtcc ggcgttttca ccgggccggg
     1021 ctcaaggtct gcggctggca gtacatctac ggcaggcggc cgatcgccga agcgaagatc
     1081 tcggccgtcg ccaagcggcg cggggcggac tgcttcgtga tcgacgccga gtccgaattc
     1141 gaaggcgcct acgctgccgc cgactggtac atccgttccc ttcgccaacg ggtcggacgc
     1201 cgtttcccga tcgggctcgc aggcttcccg tacgccaact accacccggg ctacccctat
     1261 tcggtctttc tcggcccggg ggcggcaaat gcgaatgtgc cgcaggtcta ctggcacacg
     1321 atcggggatt cggtgcggac cgcgctggag atcacctggg agcagaacag catctacaag
     1381 cggccgatct tcccggttgg ccagacctac gaggatcccg gtgcccggga gctgctcgcc
     1441 taccgccgct tcatgctcaa ctacggcacc cggccgagct ggtggtcctg gcaggaaacc
     1501 agcccggccg aatggaagat cctcgccagg ccggtgagcc gggtgtccgg ttaccgggcc
     1561 tactcgggtc atgtgacgct tgagaaggga gatcgcggtg accaggtgct ctggctgcag
     1621 cagcatctga tcgcggccgg ctactcgatg gagccgaccg ggatcttcag tcgcagcacc
     1681 tacaaggcag tccgccggtt ccaggccagg cggggtc
//
LOCUS       DAOQMF010000474         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_51832_length_1716_cov_2.332330, whole genome shotgun sequence.
ACCESSION   DAOQMF010000474 DAOQMF010000000
VERSION     DAOQMF010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51832_length_1716_cov_2.332330"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLV77_07975"
     CDS             complement(<1..472)
                     /locus_tag="PLV77_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15822.1"
                     /translation="MARAQNNIQQAKPAAPGGPLWLLRRFGWFIEKYLLWPIADSFRA
                     IGRGIARIPNAFRYRSPMAYIGVTLMLTLTAGAIAAAVYFYKQQEDKGQDPAVATTAV
                     PTETVVPSVPTPTTTPTTVEDTTPNSGDVDQTLQGVVPNFQTSGKTKQSGAGADS"
     gene            complement(677..>1716)
                     /locus_tag="PLV77_07980"
     CDS             complement(677..>1716)
                     /locus_tag="PLV77_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC15823.1"
                     /translation="NAEARLTELQAEGGTMLTEEVTEQDVAEVVAKWTGIPVTRLMEG
                     EVEKLVHMEERLHDRVIGQDEAVDAVANALRRSRAGLSDPDRPIGSFLFLGPTGVGKT
                     ELAKSLAEFMFDTDQAIVRLDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRP
                     YAVVLLDEIEKAHPDVFNTLLQLMDDGRLTDGQGRTVSFTNTVLIMTSNVGSQEIAGE
                     DVDERIRERVTEVLETTFKPEFLNRIDDTVIFHRLSKEDIGKIVEIQVEGLTRRVAER
                     DIEIELTDDARTLLGNLGYDPTYGARPLKRVVQKQLVDKLALKILEGEFTEGDSVLID
                     AAEGELAFSKK"
BASE COUNT          282 a          539 c          558 g          337 t
ORIGIN      
        1 ggctatctgc gccggcaccg ctttgtttcg tcttgccaga agtctggaag ttcgggacca
       61 cgccctgaag ggtctggtcg acatcacccg agttcggggt tgtgtcttcg accgtggtcg
      121 gggtggtggt cggcgtcgga accgagggaa cgaccgtctc ggtcggcacc gcggtggtgg
      181 cgacggcggg atcctggccc ttgtcctcct gctgcttgta gaagtagacg gcggcagcga
      241 tcgcaccggc ggtcaaggtg agcatcaggg tgacgccgat gtaggccatc gggctgcggt
      301 agcggaaagc attggggatc cgggcaatcc ctcggccgat cgcacggaag gagtccgcga
      361 tcggccagag aaggtacttc tcgataaacc agccgaagcg ccggagcagc caaagcggcc
      421 cgccgggagc ggctggcttc gcctgctgga tgttgttttg agctctcgcc atcgggtccg
      481 gggcctgctt tccgcctccg atatgcctcc ctgaggccag acgcggagcg tgcagggtaa
      541 tacgggaacc caaaagagga agtaaagaag cggttaagcg ggtgcgaaag tgcaacccgt
      601 gtctcgcatg aaacatagat ggagagccga tttcgactct ccacccacat ttcgtatcat
      661 cttgatcaag aaaaccctat ttctttgaga aggcgagttc accctcggcg gcatcgatca
      721 ggaccgaatc accctcggtg aactcccctt cgaggatctt cagggcgagc ttgtccacca
      781 actgcttctg gaccacccgc ttgagcggcc gggcaccgta ggtcgggtcg tacccgaggt
      841 tgccgagcag ggtcctggcg tcgtccgtga gctcgatctc gatgtcccgc tccgccaccc
      901 ggcgggtcag gccttccacc tggatttcga cgatctttcc aatgtcctcc ttcgagaggc
      961 ggtggaagat caccgtgtca tcgatccggt tgaggaactc gggcttgaag gtcgtttcga
     1021 gaacctcggt cacccgctcg cggatccgtt cgtcgacgtc ctcgccggca atctcctgcg
     1081 agccgacgtt cgaggtcatg atcagcaccg tgttggtgaa gctgactgtc cggccctggc
     1141 cgtcggtcag gcggccgtcg tccatcagct ggagcagcgt gttgaagacg tccggatgag
     1201 ccttctcgat ctcgtcgagc aggaccaccg cgtagggccg gcgccggacc gcctcggtca
     1261 gctggccgcc ttcttcgtat ccgacgtatc cgggaggtgc gccgatcagc cgggcgacgg
     1321 tgtgcttctc catgtactcg gacatgtcga gcctgacgat cgcctggtcg gtgtcgaaca
     1381 tgaactcggc cagtgacttg gccagctcgg tcttgccgac tccggtcggc ccgaggaaga
     1441 ggaaggagcc gatcgggcgg tccgggtccg aaagcccggc ccgcgagcgg cgcagcgcgt
     1501 tggcgaccgc atcaacggcc tcgtcctggc cgatcacccg gtcgtggagg cgctcctcca
     1561 tgtggaccag cttctcgacc tcgccctcca tcaggcgggt gaccgggatc ccggtccact
     1621 tggcgaccac ctcggccacg tcctgttcgg tgacctcctc ggtcagcatc gtgccgccct
     1681 ctgcctgcag ctcggtcagc ctggcttcgg cgttgg
//
LOCUS       DAOQMF010000475         1714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_51942_length_1714_cov_3.391802, whole genome shotgun sequence.
ACCESSION   DAOQMF010000475 DAOQMF010000000
VERSION     DAOQMF010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51942_length_1714_cov_3.391802"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /gene="ahcY"
                     /locus_tag="PLV77_07985"
     CDS             <1..1209
                     /gene="ahcY"
                     /locus_tag="PLV77_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNC15824.1"
                     /translation="TQDHAAAAVAVGPDGTVENPKGVPVFAWKGETLEEYWWCTEQAL
                     TWPGTGADGSVGPNMILDDGGDATLLIHKGVEFEKAGAVPDPAGADSEEFRIVLQTLT
                     DSLAADSSKWTEIAKGIKGVTEETTTGVHRLYEMLENGELLFPAINVNDSVTKSKFDN
                     LYGCRHSLIDGINRATDVMIAGKAAVVCGFGDVGKGCAESLRGQGARVIITEIDPICA
                     LQASMQGYEIRRLDDVVDSADIFITTTGNKDIITADHMAKMKHQAIVGNIGHFDNEID
                     MVGLEATPGIEKIEIKPQVHEYRFADGHSIIVLSEGRLLNLGNATGHPSFVMSNSFTN
                     QVIAQIELYAKADDYELGVFVLPKHLDEKVARLHLQAVGADLTELTEEQAAYIGVKVE
                     GPYKPDHYRY"
     gene            complement(1515..>1714)
                     /locus_tag="PLV77_07990"
     CDS             complement(1515..>1714)
                     /locus_tag="PLV77_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15825.1"
                     /translation="YAGPKRQFITLAPWVRKDGSRAELRKTGAALLAASGKKMENRYL
                     ETAAIADLPFPPKKHRANCVS"
BASE COUNT          333 a          585 c          527 g          269 t
ORIGIN      
        1 acccaggacc acgccgccgc cgcggtcgcg gtgggcccgg acggcaccgt cgagaacccc
       61 aagggtgtgc cggtcttcgc ctggaagggc gagaccctgg aggagtactg gtggtgcacc
      121 gagcaggccc tgacctggcc cgggacgggc gccgacggtt cggttggtcc gaacatgatt
      181 ctcgatgacg gcggtgacgc gaccctgctg atccacaagg gagtcgagtt cgagaaggcc
      241 ggagccgtgc cggatccggc cggagccgac tccgaggaat tccgcatcgt cctgcagacc
      301 ctgaccgact cgcttgccgc ggacagcagc aagtggaccg aaatcgcaaa gggcatcaag
      361 ggcgtgaccg aagagaccac caccggggtc caccggctct acgagatgct ggaaaacggc
      421 gagctcctct tcccggcgat caacgtcaac gactcggtca ccaagtccaa gttcgacaac
      481 ctctacggct gccgccactc gctgattgac ggaatcaacc gggccaccga cgtgatgatc
      541 gccggcaagg ccgcggtcgt ctgcggtttc ggcgacgtcg gcaagggctg cgccgaatca
      601 ctgcgcggtc agggtgcccg ggtgatcatc accgagatcg acccgatctg cgcccttcag
      661 gcttcgatgc agggttacga gatccgccgc ctcgatgacg tcgttgactc ggccgacatc
      721 ttcatcacga ccaccggcaa caaggacatc atcaccgccg accacatggc gaagatgaag
      781 caccaggcga tcgtcggcaa catcggccac ttcgacaacg agatcgacat ggtcggcctc
      841 gaagcgaccc ccggcatcga gaagatcgag atcaagccgc aggtccacga ataccgtttc
      901 gccgacggcc actcgatcat cgttctttcc gaaggccggc tgctcaatct cggcaacgcc
      961 accggccacc cgagcttcgt gatgtcgaac tccttcacca accaggtgat cgcccagatc
     1021 gagctctacg ccaaggcgga cgattacgaa ctgggcgtct tcgtcctgcc caagcacctc
     1081 gacgagaagg ttgcccgtct ccacctccag gcggtcggcg cagacctcac cgaactgacc
     1141 gaggaacagg ccgcctacat cggcgtcaag gtcgaaggcc cctacaagcc ggatcactac
     1201 cggtactagc ccagcaggcc ccagcaggac ccggcggtcc tggggcggcc gctggcatcc
     1261 acccgctgaa gtgcgggaga tattcccatt ccgcgagatc ccggtttctg ccggatagct
     1321 cccccaactc cgatctcgcc cgtctcccga cccagatccg cggtaggtgc cgcacgttta
     1381 ggcccatcaa gggcccaaac gcgcggcacc tcccgaaaca ctttgtgccg gtaggcggca
     1441 caaagtgggc tttcgggacc acaagcctcc cgctgatgcc cgcccgaacc cgaggtgcaa
     1501 ctgcagatgc gcaatcagct aacgcagttg gcccggtgct tctttggcgg gaacgggagg
     1561 tcggcgatgg cggctgtttc gaggtagcgg ttctccatct tcttgccgct ggccgccagc
     1621 agggcagcgc cggtcttgcg cagctcggcc cgggagccgt ccttgcggac ccagggcgcc
     1681 agggtgatga actggcgctt ggggccggca tacc
//
LOCUS       DAOQMF010000476         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_52161_length_1710_cov_3.528701, whole genome shotgun sequence.
ACCESSION   DAOQMF010000476 DAOQMF010000000
VERSION     DAOQMF010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52161_length_1710_cov_3.528701"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1288
                     /locus_tag="PLV77_07995"
     CDS             182..1288
                     /locus_tag="PLV77_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC15826.1"
                     /translation="MMLVAELQELEEVKTLVNRGQQLGVLTFGDVATAVAEVDLDESD
                     VEDLYGHIEKQGIELVEDVDPAQKASAENERSDGRRGRRRKKEALDLKPDMTTDSLQL
                     FLKDIGKVRLLTAQEEVDLAKRIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLD
                     LIQEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLN
                     KIGRAERKLVTELGREPTPEEIAEVTGIDPEEVDSIKRSAQSPVSLEKPVGDEEESEF
                     GQFIADEKAESPFDRAADLLTKEALKESLENLSYRERRVLELRYGLGGEHPRTLDEVG
                     RTFNVTRERIRQIENQSLKKLQSLNEAQKLREAT"
     gene            1401..>1710
                     /locus_tag="PLV77_08000"
     CDS             1401..>1710
                     /locus_tag="PLV77_08000"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNC15827.1"
                     /translation="MAVPVLVITLLIASATDLRSREIPNWLMAGAALAGIGLAAAEGR
                     AGSAVLFGLLAAAPFLLANLIRPEGMGMGDVKLVAVVGLYLGQQVWIALVIGLALAGL
                     T"
BASE COUNT          330 a          537 c          536 g          307 t
ORIGIN      
        1 aagtggctat tatccggtca ctcgtttgac cacttacaga cggcggcggt ttttcctgct
       61 cctgtttaca tatggagcga tgcggatcct gacgagtgat tcgcaagatt ttctgtgacc
      121 cggaccctca ttttcgggta gacaggtata gacgccgtct tacggaatac cagataggga
      181 catgatgctt gtagccgaac tccaggaact agaagaagta aagaccctcg tcaacagggg
      241 tcagcagctc ggcgtcctca ctttcggtga cgttgccact gccgtggctg aggtcgatct
      301 cgacgaatca gacgtcgaag acctttacgg tcacatcgaa aagcagggga tcgagctggt
      361 cgaggatgtg gatccggcgc agaaggcttc ggccgagaac gagcgctccg acggccgccg
      421 cggccgccgg cgcaagaagg aggcgctcga cctcaagccg gacatgacca ccgactcact
      481 gcagctcttc ctcaaggaca tcggcaaggt tcgcctgctg accgcccagg aagaagtcga
      541 tctcgccaag cggatcgagc gcggtgacct cgacgccaag cagaagatgg tcgagtcgaa
      601 cctccggctc gtcgtctcga tcgccaagaa ctaccgcaac cagggcctgc ctttcctcga
      661 tttgatccag gaaggcacgc tcggcctggt ccgtgcggcc gagaagttcg attaccgcaa
      721 gggattcaag ttctcgacct acgcgacctg gtggatccgc caggcgatcg cccgcgccct
      781 cgccgacaag gcccggacca tccggatccc ggtccacgtt gtcgagaagc tgaacaagat
      841 cggccgtgcc gagcgcaagc tggtgacgga actgggccgc gagcccaccc cggaggagat
      901 cgccgaagta accggcatcg accccgagga agtcgactcg atcaagcgtt ccgcccagtc
      961 acccgtctcg ctcgagaagc cggtcggtga cgaggaggaa tccgagttcg gccagttcat
     1021 tgccgacgag aaggccgagt cgccctttga ccgggcagcc gacctgttga ccaaggaagc
     1081 gctgaaggaa tcgctggaga acctctccta ccgcgagcgc cgcgtgctcg agctccgtta
     1141 cgggctcggc ggcgagcacc cccgcaccct cgacgaggtc gggcgcacct tcaacgtgac
     1201 ccgcgagcgg atccgtcaga tcgagaatca gtcgctgaag aagctccagt cgctgaatga
     1261 agcgcagaag cttcgtgagg ccacttgatc ctcaggatct gaatcaactg cgaaaggcca
     1321 gcctccgggc tggcctttct gttgcacaat cgttgcacct gtagagccac gtgtgaggtt
     1381 ttatcgggca gggtcggggt atggccgttc ccgtcctcgt catcacgctc ctgatcgcct
     1441 cggcgaccga tttgcgcagc cgcgagatcc cgaactggct gatggccggg gcggccctgg
     1501 ccgggatcgg actggcggcc gccgagggcc gggccggctc cgcggtcctg ttcggcctgt
     1561 tggcggccgc accgttcctg ctcgcgaacc tgatccggcc cgaaggcatg ggcatgggtg
     1621 acgtcaagct ggtggcggta gtcggcctct acctgggcca gcaggtctgg atcgctctcg
     1681 tcatcggtct cgccctggcc ggcctgaccg
//
LOCUS       DAOQMF010000477         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_52486_length_1705_cov_2.570303, whole genome shotgun sequence.
ACCESSION   DAOQMF010000477 DAOQMF010000000
VERSION     DAOQMF010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52486_length_1705_cov_2.570303"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PLV77_08005"
     CDS             <1..1222
                     /locus_tag="PLV77_08005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC15828.1"
                     /translation="LKCAGLLADAGHEVLVFEASDGVGGRVRTHEVDGFLLDRGFQVL
                     LAAYPEVPRTFDLEALDPGEFDPGALVRLNGRFSPLLDPLRRPAKTIAAVSSPVIGIA
                     DKLRVARMRQELLGKEPAEVLSRPDRTALESLKGRGFSPNAIERFFRPFFGGVFIDPD
                     LGTSSRLMEIFFRCFSAGPAVLPKDGMGQLAASMAARLPEGSLQLGSPVREVRADGVL
                     PTDGDWIEALAVVVATDERSASRLAGLPEPEPGRVTSCVYFDAPAGEISGRTLYLSPR
                     AAGPINELAVPSAVSAGYAPAGRSLVSVSAIGENARRDDLEDAVSAQLGEWFGAATVA
                     GWRHLGTRTVEDALPDFGPGRFRAGGQDPALDSGLFVCGDYRESPSIQGALVSGRKAA
                     AAVRAKLARARTEG"
     gene            complement(1335..>1705)
                     /locus_tag="PLV77_08010"
     CDS             complement(1335..>1705)
                     /locus_tag="PLV77_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576296.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNC15829.1"
                     /translation="QLLGLTAPEMTVLVGGLRVLGANTGGSKHGVLTDSPETLTNDFF
                     VNLLSFETEWKPADEDSTTFEAPGKGWTGTRVDLVFGSNSELRALAEVYASEDAKDKF
                     VTDFIFAWTKVMNADRFDLD"
BASE COUNT          253 a          547 c          581 g          324 t
ORIGIN      
        1 cctgaaatgc gccgggcttc tcgccgatgc cggccatgaa gttctcgtgt tcgaagcctc
       61 agacggggtc gggggccggg tccgcaccca cgaggtcgat ggctttctgc tcgaccgcgg
      121 cttccaggtg ctgcttgccg cttacccgga ggttccccgg acctttgatc tcgaggccct
      181 tgacccgggt gaattcgatc cgggggcgct ggtccggcta aatggaaggt tttcgccact
      241 gcttgatccc ctccgccgcc ccgccaagac gatcgccgcg gtgagttcgc cggtgatcgg
      301 cattgccgac aagctgcgcg ttgcccgcat gcgtcaggag ctgctaggca aagagccagc
      361 cgaggttctt tcccgtcctg accggaccgc actcgaatcc ctgaagggca ggggcttttc
      421 accgaacgcg atcgaaaggt tctttcgccc ctttttcggc ggagtgttca tcgacccgga
      481 cctggggact tcatcgcgcc tcatggagat cttcttccgc tgcttttcgg ccggtcccgc
      541 ggtcctgccg aaggacggca tggggcagct ggccgccagc atggccgcgc ggcttccgga
      601 aggctcgctt cagctcggct ccccggttcg cgaggttcgg gccgatggcg tcctgcccac
      661 cgacggggac tggatcgaag cgctagcggt ggtagtcgcg accgacgagc gttccgcttc
      721 gagattagcc ggcctgcccg agcccgaacc gggcagggtg acgagttgtg tctacttcga
      781 cgctcccgct ggcgagataa gcggcagaac cctttatctc tcgccgaggg ccgcagggcc
      841 gatcaacgag ctcgccgttc ccagtgcggt cagcgcgggt tatgccccgg caggaaggtc
      901 gctggtttcg gtcagcgcga tcggggaaaa cgcccggcgt gacgacctcg aggatgctgt
      961 gagcgcccag ctaggcgagt ggtttggagc tgccacggta gccggctggc gccacctcgg
     1021 gactcgcacc gtcgaggacg cgctgcccga cttcgggccg ggccgcttcc gggccggcgg
     1081 ccaggatccg gcacttgatt cggggctttt cgtctgcggg gattaccgcg agtcgccctc
     1141 gatccaagga gctcttgtct cgggccgcaa ggcggccgcg gcggtgcggg caaagctggc
     1201 tcgcgccagg accgaaggct gaacctcgcg cagaaacctc tttgccggag gtttctgcgc
     1261 tcggtcggtg caaatcgaga tggcagtttg gggcgataat gggccacttc tgccatctcg
     1321 atccctgcgg ttgatcagtc gaggtcgaag cggtccgcgt tcatcacctt cgtccaggcg
     1381 aagatgaagt cggtcacgaa cttgtccttc gcgtcctcgg aggcgtagac ctcggccagg
     1441 gcgcggagtt ccgagttcga gccgaagacc aggtcaaccc gggtaccggt ccatcccttg
     1501 ccgggggcct cgaaggtggt cgagtcctcg tcggccggct tccattccgt ctcgaaggag
     1561 aggaggttca cgaagaagtc attcgtgagg gtctccgggc tgtcggtcag gacgccgtgc
     1621 ttcgagccgc cggtgttggc gcccagcacc cgcaggccgc cgaccagcac ggtcatctcg
     1681 ggcgcggtca ggccgagcag ttggg
//
LOCUS       DAOQMF010000478         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_52604_length_1703_cov_2.309466, whole genome shotgun sequence.
ACCESSION   DAOQMF010000478 DAOQMF010000000
VERSION     DAOQMF010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52604_length_1703_cov_2.309466"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLV77_08015"
     CDS             <1..289
                     /locus_tag="PLV77_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15830.1"
                     /translation="IPHKTFLGNRPTSTIMVDRLTPKSLGSLVALYEHSVFTQGAIWG
                     IDSFDQWGVELGKALAQAIIPELQSEEPPPLDHDSSTAELIRRYREANGRR"
     gene            357..1070
                     /locus_tag="PLV77_08020"
     CDS             357..1070
                     /locus_tag="PLV77_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC15831.1"
                     /translation="MTASVEAPIRAVIFDLDGTLLTTGGAGAVAWDKAFQEVFGKSVD
                     IAKVTESGMTDHDVAFAALRTVLDREPSHQDIDELTPVYVRELPEAVASSEKYRIEPG
                     IVELLERLASEGIPLGMTTGNIEPAARIKMERGDLNRFFAFGGYGSDSPNRAELTLHA
                     IKRGVVTCATGSFPNGRPQVLSKDDFIAVGDTPRDVSAAHEAGIRIVSVATGLFDLET
                     LKAAGPEWALPTVEEGFPV"
     gene            complement(1173..>1703)
                     /locus_tag="PLV77_08025"
     CDS             complement(1173..>1703)
                     /locus_tag="PLV77_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNC15832.1"
                     /translation="PVVPKELLTGNTRYLVNPTGRFVVGGPHGDCGLTGRKIIVDTYG
                     GAARHGGGAFSGKDPSKVDRSAAYAARYAAKNVVASGLADRCEVQVAYAIGVARPVSI
                     MAETYGTGKKSDWEISQLVADNFDLRPGAFRQYLDLHRPIYKDTAAYGHFGRSNENFT
                     WERTDRAESLKEAAGL"
BASE COUNT          304 a          572 c          543 g          284 t
ORIGIN      
        1 gatcccccac aagaccttcc tcggcaaccg gccgacctcg acgatcatgg ttgaccgcct
       61 gaccccgaag tcgctcggtt ccctggtcgc cctctacgag cattccgtct tcacccaggg
      121 tgcgatctgg ggcatcgact ccttcgacca gtggggggtc gaactgggca aggccctggc
      181 ccaggcgatc atccccgaac ttcagtccga agagccaccg ccgcttgatc acgacagttc
      241 gaccgcggag ctgatccgtc gctaccgcga ggcaaacggc cgccgatgaa atcgagaagg
      301 caaaactggc catatagcgc tccattcagc cttctcgttg ccggggacgg ctgcccatga
      361 ccgcctcggt cgaggccccg atccgcgcgg tcattttcga cctcgacggc accctgctga
      421 ccaccggcgg ggccggcgcg gtcgcctggg acaaggcctt tcaggaagtg ttcggcaagt
      481 cggtcgacat cgccaaggtg accgagtcgg gcatgaccga ccacgatgtc gccttcgctg
      541 cccttcgcac cgtgctcgac cgcgagccaa gccatcagga catcgacgaa ctgaccccgg
      601 tctatgtccg cgagctcccg gaggcggtcg ccagttcgga gaagtaccgg atcgaaccgg
      661 gaatcgtcga actgctggaa aggcttgcgt ccgagggaat cccgctcggc atgaccaccg
      721 gaaacatcga gccggcagcc aggatcaaga tggagcgagg tgacctcaac cgcttcttcg
      781 ccttcggcgg ttacgggtcg gactcaccca accgggccga actcaccctt cacgcgatca
      841 agcgcggcgt ggtgacctgc gccaccggca gcttccccaa cggccggccc caggtcctct
      901 ccaaggacga tttcatcgcg gtcggcgaca ccccccgcga cgtctccgcc gcccacgaag
      961 ccggcatccg catagtcagc gtcgccaccg gcctcttcga cctggaaaca ctgaaagcag
     1021 caggacccga gtgggccctg ccgacggtcg aagaaggctt ccctgtctga gtgcaattgg
     1081 ggcgggttcg acccgctcaa gatcgagatg gcagaagtca gcggatatga ctgacttctg
     1141 ccatctcgat tcgaaacgtg tcgcgaaccg ccttacaggc cggcggcttc cttcagggac
     1201 tcggccctgt cggtgcgctc ccaggtgaag ttctcgtttg agcggccgaa gtggccgtag
     1261 gcggcggtgt ccttgtagat cgggcggtgg aggtcgaggt actggcggaa ggcaccgggc
     1321 cggagatcga agttgtcggc gaccagctgg gaaatctccc agtcgctctt cttgccggtg
     1381 ccgtaggtct cggccatgat cgaaaccggg cgggcgacgc cgatcgcgta ggcgacctgg
     1441 acctcgcagc ggtcggcgag gccggaggca accacgttct tggcggcgta acgggctgcg
     1501 taggcggccg aacggtccac cttcgacgga tccttgcccg agaaggcgcc accgccgtgc
     1561 ctggcggctc cgccgtaggt atcgacgatg atcttgcggc cggtcagccc gcagtcgccg
     1621 tgcggaccgc cgacgacgaa acgcccggtc gggttgacca ggtagcgggt gttgccggtc
     1681 agcagttcct tcggaacgac cgg
//
LOCUS       DAOQMF010000479         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_52765_length_1700_cov_3.578723, whole genome shotgun sequence.
ACCESSION   DAOQMF010000479 DAOQMF010000000
VERSION     DAOQMF010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52765_length_1700_cov_3.578723"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLV77_08030"
     CDS             <1..996
                     /locus_tag="PLV77_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15833.1"
                     /translation="VKASPEHPLLLDRFLENAIEVDVDALCDGEEVFVAGIMQHVEEA
                     GVHSGDSACVLPPMSIGQEMIDEIKQATTAIALELGVIGLINIQYALAGGELYVIEAN
                     PRASRTVPFVSKAIGIPLAKVATRIMLGQKLADQDLPEFTGDHVSVKEAVLPFSRFQG
                     ADSVLGPEMKSTGEVIGIGPDFPTAFGKAQAAAGVSLPESGAVFISVTDTDKAAATQL
                     AAQLHDLGFEILATTGTAQAISRMGTPVRAINKIAEGSPHVVDMIEEQHCDLVINTPT
                     GSGARADGYEIRSAAIRHGIPSVTTLTGASAAVRAMAARANGSGEVKSLQELHAR"
     gene            986..>1700
                     /locus_tag="PLV77_08035"
     CDS             986..>1700
                     /locus_tag="PLV77_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15834.1"
                     /translation="MPADPAATPDRVQAPFGRRLCEVAETRPSGGYRIFTLIDPEGPA
                     PAPGQFFMLAAAPDWAREGGRPYLPRAISHARFTRHPGSGQRLDFLIDEVGPGTSRLA
                     EMKPGDQCWVAGPFGGRFSLPRKLNPQAAGAILVGGGIGIAPMAVLRRELVDRGIPSR
                     SLLGFRDQAHSGGVEELFGCEETRLAFEDGSSGHKGYVTDLLLQLLEGDDSASAAVYS
                     CGPPAMLEAGNRAVAEFVEY"
BASE COUNT          265 a          583 c          576 g          276 t
ORIGIN      
        1 gtcaaggcct cgccggaaca tccgctgctg ctcgaccgct tcctcgagaa cgcgatcgag
       61 gtcgacgtgg acgcactctg cgatggcgag gaggtcttcg tggccgggat catgcagcac
      121 gtcgaagagg ccggagtcca ttccggcgac tcggcctgcg tgctgccgcc gatgagcatc
      181 ggccaggaga tgatcgacga gatcaagcag gcgaccacgg cgatcgcgct cgaactggga
      241 gtgatcggcc tgatcaacat ccagtacgcg ctggccggcg gcgagcttta cgtgatcgag
      301 gccaatcccc gggcctcgcg gaccgtgcca ttcgtttcga aggcgatcgg gattcccctg
      361 gccaaggtcg cgacccggat catgctcggc cagaagctgg ccgaccagga cctgcccgaa
      421 ttcaccggtg atcatgtctc ggtcaaggag gcggtgctgc cgttttcccg cttccagggt
      481 gccgattcgg tgctcggtcc ggagatgaag tcaaccggtg aggtgatcgg gatcggcccc
      541 gacttcccga ccgccttcgg caaggcccag gcggcggccg gggtatccct tccggaatcc
      601 ggggccgtgt tcatctcggt caccgacacc gacaaggctg ccgccaccca gctcgccgcc
      661 cagcttcacg accttggttt cgagattctc gccacgaccg gcaccgccca ggcgatctcg
      721 cgcatgggaa ccccggtcag ggcgatcaac aagatcgccg agggctcccc gcatgtggtc
      781 gacatgatcg aggagcagca ctgcgacctg gtgatcaaca cgccgaccgg ttccggggcc
      841 cgggccgacg gttacgagat ccgctcggcc gcgatccggc acggcattcc ttcggtgacc
      901 acactgaccg gggcctcggc tgcggtccgg gcgatggccg cccgggccaa tggttccggg
      961 gaggtcaagt cacttcagga gcttcatgcc cgctgacccg gccgccacgc ctgaccgggt
     1021 tcaggcgccg ttcggacggc gactctgcga agtcgctgaa acgaggccct cgggcggcta
     1081 ccggatcttc acgctgatcg accccgaagg cccggccccg gccccgggcc agttcttcat
     1141 gctggccgcc gcgcccgact gggcgaggga gggcggccgt ccgtacctgc cgcgggcgat
     1201 ctcccatgcc cggttcacac ggcaccccgg ctccgggcag cgacttgact tcctgatcga
     1261 cgaggtcggc ccgggaactt cgaggctcgc ggagatgaag ccgggagatc aatgctgggt
     1321 ggccgggccg ttcggtggcc gcttcagcct gcctcgcaag ctcaatccgc aggccgccgg
     1381 ggcgatcctg gtcggcggcg ggatcggcat cgcgccgatg gcggtgctgc gccgtgaact
     1441 cgtcgaccgc gggattccct cccgcagcct gctcggattc cgtgatcagg cgcattccgg
     1501 cggggtggaa gaactcttcg gctgcgagga gacgaggctc gccttcgagg acggcagctc
     1561 cggccacaag ggttacgtga ccgacctgct gctccagctg ctcgaaggtg acgattccgc
     1621 ctcggccgcc gtctactcct gcggaccgcc ggcgatgctg gaggccggca accgcgccgt
     1681 cgcggagttc gtcgagtacc
//
LOCUS       DAOQMF010000480         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_52779_length_1700_cov_3.009726, whole genome shotgun sequence.
ACCESSION   DAOQMF010000480 DAOQMF010000000
VERSION     DAOQMF010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52779_length_1700_cov_3.009726"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLV77_08040"
     CDS             <1..206
                     /locus_tag="PLV77_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15835.1"
                     /translation="FDNDRVGGIVILVSGGYDKSLLPDLKLAGANRFIKTIDCHSRLE
                     QIVGEAHLERLFMREARQKNSGF"
     gene            206..364
                     /locus_tag="PLV77_08045"
     CDS             206..364
                     /locus_tag="PLV77_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15836.1"
                     /translation="MVTLTVIILIIQVATFLALGGVFMATGHMRLGIAQILLAAVQVV
                     IYSEGITR"
     gene            473..940
                     /locus_tag="PLV77_08050"
     CDS             473..940
                     /locus_tag="PLV77_08050"
                     /inference="COORDINATES: protein motif:HMM:NF020302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HNC15837.1"
                     /translation="MNGTTTLPWEEISGFTGIHQYLSNFWEACVTMEGKVYPTTEHAF
                     QAAKTLDPDERRQIRMEVTPGRAKRLGRKVALRHDWDEIKEGVMLELVRQKFTTHPEL
                     ARRLIHTGNARIVEENHWNDTLWGVCDGVGENRLGEILMKVRSELRDLAGEIS"
     gene            943..1203
                     /locus_tag="PLV77_08055"
     CDS             943..1203
                     /locus_tag="PLV77_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15838.1"
                     /translation="MADQTPGGWLDQLTVQERIRTGNPNLVLRPIEAAALLAQGLMRG
                     DPATFKQGYTRENAVIAAQEMYPEFTRQQISDQLAEFETNQP"
     gene            1247..>1700
                     /locus_tag="PLV77_08060"
     CDS             1247..>1700
                     /locus_tag="PLV77_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15839.1"
                     /translation="MGANMIAATIWLAQGTEPDWEKAETTLAGFTCPAGGFPEDHDLS
                     DTIVEDMILGEYEDKEASTDKQRKEIVDGILKGAYQVVKDGVEGKRRDMTFSVFAGWV
                     VYISGGLSWGDAPTDAEEAIRNLSGTGILEAAGFNTRPEKVMAVVLESK"
BASE COUNT          399 a          518 c          524 g          259 t
ORIGIN      
        1 agttcgacaa cgaccgggtc ggtggcatcg tgatcctggt ctccggcgga tacgacaagt
       61 cgcttctgcc tgacctcaag ctggccggtg cgaaccggtt catcaaaacg atcgactgcc
      121 actccaggct ggagcagatc gtcggggaag cccacctgga acgcctgttc atgcgggaag
      181 cccgtcagaa aaacagcgga ttctgatggt caccttgaca gtgatcatcc tgatcatcca
      241 ggtagccacg ttcctcgccc ttggcggggt gttcatggcg accgggcaca tgcggctcgg
      301 gatcgcccag atcctgctcg cagcagtcca ggtggtgatc tactcggaag gaatcaccag
      361 gtaacacggc atcgggaccg attcccgaaa cgaacatgaa gggctgccac ccggcgcaag
      421 tcaggggacg gccctttttt cgtggccaag tgccacagaa agaaggtgcg ccatgaacgg
      481 cacaacaacg ctcccctggg aggaaatctc cggtttcacc ggcatccacc agtacctgag
      541 caacttctgg gaggcctgcg tgacgatgga agggaaggtt tacccgacca ccgagcatgc
      601 cttccaggca gcgaaaacac tcgatccgga cgaacgccgg caaatccgaa tggaagtcac
      661 acccggccgc gcgaaaaggc tcggacggaa agtggcgctt cgccacgact gggacgaaat
      721 caaggaaggg gtgatgctgg agctggtccg ccagaagttc accacccatc cggaactcgc
      781 ccgccggctg atccacaccg gcaacgcccg catcgtcgaa gaaaaccact ggaacgacac
      841 cctttggggt gtttgcgacg gggtcgggga aaaccggctg ggggagatcc tgatgaaggt
      901 ccgctccgag cttcgcgacc ttgccgggga gatcagctga ccatggccga tcaaaccccc
      961 ggaggctggc ttgaccagct caccgtccag gaacgaatcc ggaccggaaa cccgaacctg
     1021 gtgcttcggc caatcgaagc ggccgcgctc ctcgcacaag gactcatgag aggtgacccg
     1081 gccacgttca agcagggcta cacccgagaa aacgcggtga tcgcagccca ggagatgtac
     1141 ccggagttca cccggcagca gatcagcgat cagctcgccg aattcgaaac caaccagccc
     1201 tgaagggcta aacctgcggc agacgccgca agaaacggag gctcccatgg gagcaaacat
     1261 gatcgcggca accatctggc tggcgcaagg caccgaaccc gactgggaga aagccgaaac
     1321 gaccctggcc gggttcacct gtcccgccgg cgggttcccg gaagaccacg atctttcgga
     1381 caccatcgtc gaggacatga tcctcggcga atacgaggac aaggaagcga gcaccgacaa
     1441 gcagcgcaag gaaatcgttg acggcatcct gaaaggcgcc taccaggtcg tcaaggacgg
     1501 tgttgaaggc aagcggcggg acatgacgtt ctcggtgttt gccggctggg tcgtttacat
     1561 cagcggcggt ctcagctggg gggatgcccc caccgacgcc gaagaggcga tcaggaacct
     1621 ctcgggaacc ggcattctcg aagcggccgg attcaacacc cggcccgaga aggtgatggc
     1681 ggtggttctc gaatcgaaat
//
LOCUS       DAOQMF010000481         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_52813_length_1699_cov_4.127737, whole genome shotgun sequence.
ACCESSION   DAOQMF010000481 DAOQMF010000000
VERSION     DAOQMF010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52813_length_1699_cov_4.127737"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLV77_08065"
     CDS             complement(<1..1120)
                     /locus_tag="PLV77_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15840.1"
                     /translation="MKSKLIAAGAVLVAVLLTGVLGAGAASASSAKPPFYPIDKKTIK
                     VPNDVQPWLPTYSPDGKHIVFQNQLDGSTWATSKDGKGTSCITCEFSDRPDISGGFTY
                     VFPDNKRLFVSHELGGLTGVDSGPNTDAWILECSPSIYDCQSHQYLPVDMSDDKVGVP
                     LIVQRRTWHLAPDGVHLGWMDIRLDGTVMIVGRLTRLDDRYKVVDQKAVNPYRPTSLT
                     DTDAANWAGDNQLWELKSFADGGASILALGGAGYNIDPVKINLKTGKVTRLTANPDWD
                     EDGAISPDGKMLALYSWRTRHRLDAFGWIPQIDSFVEMPAGAALAPFYVSTWPGFQCD
                     LSPWLLSSSGDMGGKLIGQPLNVYPENNLTPGNNLSGNQ"
     gene            1250..>1699
                     /locus_tag="PLV77_08070"
     CDS             1250..>1699
                     /locus_tag="PLV77_08070"
                     /inference="COORDINATES: protein motif:HMM:NF037470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HNC15841.1"
                     /translation="MEAATTSTSKAFAAHQDEAAGLGAPRATPADAFRAALRAFVRGP
                     RLDMEKLAQDLGISKTTLYRWTGPRDKLLSEILCFFGEEAARIGREASEGATGVRRIE
                     LFTRGFVESIVNFEPHRRFVRSETELAFRLVLTRGSQVQSTVVERFAE"
BASE COUNT          289 a          540 c          554 g          316 t
ORIGIN      
        1 actggttgcc cgaaaggttg ttgcccgggg tcaggttgtt ttccgggtag acgttcagcg
       61 gctggccgat cagcttgccg cccatgtcgc cggacgagga gagcaaccag ggcgagaggt
      121 cacactggaa gccgggccag gtcgagacgt agaagggagc gagtgcggcc ccggccggca
      181 tctcgacaaa cgaatcgatc tgcgggatcc agccgaaggc gtccaggcga tggcgggtgc
      241 gccatgagta gagagccagc atcttgccgt ccggcgagat ggcgccgtcc tcgtcccagt
      301 cggggttggc ggtcaggcgg gtgaccttgc cggtcttcag gttgatcttg accgggtcga
      361 tgttgtagcc ggcaccgccc agggccagga tcgaggcgcc gccgtcggcg aagctcttga
      421 gctcccagag ctggttgtca ccggcccagt tggcggcgtc ggtatcggtc agcgaggtcg
      481 gccggtacgg attgaccgcc ttctggtcaa ccaccttgta gcgatcgtcc agccgggtca
      541 gacgaccgac gatcatcacg gtgccgtcga gccggatgtc catccagccg aggtggacgc
      601 cgtccggcgc gaggtgccag gtccgtcgct gaacgatcag cggcacgcca accttgtcgt
      661 ccgacatgtc gacgggcagg tactggtggc tctggcagtc gtagatgctc ggcgagcatt
      721 cgagaatcca ggcgtcggta ttggggccgc tgtcaacccc ggtgagcccg ccgagttcat
      781 ggctcacgaa gagacgcttg ttgtccggga agacataagt gaagccgccg ctgatgtcgg
      841 gccggtccga gaactcgcag gtgatgcagc tggtgccctt tccgtccttc gaggtggccc
      901 aggtactgcc gtcaagctga ttctggaaga cgatgtgctt gccgtccggg ctgtaggtcg
      961 gcaaccaggg ctggacgtcg ttcggcacct tgatcgtctt cttgtcgatc gggtagaagg
     1021 gcggcttggc ggagctggcc gacgccgctc ccgcccccag aaccccggtc agaagtactg
     1081 cgacgagcac ggcccctgcc gcgatcagct tgcttttcat cccaactctc tccatttccg
     1141 gcgcaagtag acgcgtcgtg tttccgattc gattgtgtac aaatatcata ttcgtacact
     1201 ttcgtgcaac tccacctttc cgtaacaccg atctgtagag ttcgattcga tggaagcagc
     1261 gaccacgtca acctcgaagg cattcgccgc ccaccaggac gaagctgccg gcctcggcgc
     1321 accccgcgcg accccggccg acgccttccg cgccgccctg cgggcattcg tccgcggccc
     1381 ccggctggac atggaaaagc tggctcagga tctcggcatt tcaaagacca ccctctaccg
     1441 ctggaccggc ccccgggaca agctgctctc ggagatcctc tgtttcttcg gcgaggaggc
     1501 ggcccggatc ggccgcgaag cgtcagaggg ggcgaccggg gtcaggcgga tcgagctctt
     1561 cacccgcggg ttcgtcgaat cgatcgtcaa cttcgagccg caccgcaggt tcgtccgcag
     1621 cgaaaccgaa ctcgccttcc gcctcgtcct gacccgcggc agccaggttc agtcgaccgt
     1681 ggtcgagcgc ttcgcagaa
//
LOCUS       DAOQMF010000482         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_52866_length_1698_cov_4.440049, whole genome shotgun sequence.
ACCESSION   DAOQMF010000482 DAOQMF010000000
VERSION     DAOQMF010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52866_length_1698_cov_4.440049"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLV77_08075"
     CDS             complement(<1..606)
                     /locus_tag="PLV77_08075"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNC15842.1"
                     /translation="MGILEQLISAASQGVKARSQQVPLADLEARLGERDHDRPFQEAL
                     TRPGMSLICEYKRKSPSAGEIAPGVELPDQVKAYEAGGAAALSVLTDETHFDGRLDDL
                     AAARAACSLPILRKDFVVDQYQLYEAAAGGADAVLLIAAVLEDDRLRDFQLKAAELDL
                     DCLVEVHDEEELERAVESGAEVIGINNRNLDTFEVSLETTFE"
     gene            complement(623..1642)
                     /gene="trpD"
                     /locus_tag="PLV77_08080"
     CDS             complement(623..1642)
                     /gene="trpD"
                     /locus_tag="PLV77_08080"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNC15843.1"
                     /translation="MPNDILTRAIDAAASGQHLTTDHASAVLTEIMEGRSDEVQTAAF
                     LIALRTKGETVPELIGLARTMRSLAEPVEVSGDLVDTAGTGGGPSTFNVSTTAALVAA
                     GAGCPVAKHGNRSSTSLSGSADLLEALGVDIEITPEKVAASIEETGFGFMFAPRHHKA
                     MAHVVPVRKALAVRTIFNFLGPLTNPAGARRQLMGVSDRRYQESIAEALAGLGTERAL
                     VVSGDDGIDEISITGPTRIIEVVDGGTSEWFVSPGDFELERAELGDVAGGTPDENAAA
                     TRAVLEGEKGPRRDLVVLNAAAAILVGGKAGDFAAGVVAAQEAIDSGAAAAVLARLVS
                     FTGQS"
BASE COUNT          277 a          568 c          561 g          292 t
ORIGIN      
        1 ctcgaaggtg gtctccaggg agacctcgaa ggtgtcgagg ttgcggttgt tgattccgat
       61 cacctcggcc ccggattcaa ccgcccgttc gagctcttcc tcgtcatgga cctcgaccag
      121 gcaatcgaga tcgagctcgg ccgctttgag ctggaaatcg cgcaggcgat cgtcttcgag
      181 cacggcagcg atcagcagga ccgcgtcggc gccgccggcc gcggcttcgt agagctggta
      241 ctggtcgacg acgaagtcct tgcgcaggat cggcaggctg caggcggcgc gggcagcggc
      301 gagatcatcg agccggccgt cgaagtgggt ctcgtcggtc agcacggaca gagcggcggc
      361 gccaccggcc tcataggcct tgacctgatc gggaagctcg accccggggg cgatctcccc
      421 ggcgctcggt gacttgcgct tgtactcgca gatcagcgac atgccgggac gggtcagcgc
      481 ttcctggaac gggcggtcat ggtcacgctc gcccagcctt gcctccaggt cagcaagggg
      541 cacctgctgg ctgcgggcct tgacgccctg gctcgcggcc gaaatcagtt gctcgagtat
      601 tcccaattcg cttcagagcc tactaggact gaccggtgaa actgaccagc cgggccaaaa
      661 cggcagcggc cgccccggag tcgatcgctt cctgtgccgc aaccacgccg gcggcgaaat
      721 caccggcctt gccgccaacc aggatcgccg ccgccgcgtt gaggaccacg aggtcacgcc
      781 tcggaccctt ttcaccttcc agcactgccc gcgtggcggc cgcgttttca tccggggtgc
      841 cgcccgcgac atcaccgagt tcggcccgct caagttcgaa atcgccgggt gagacgaacc
      901 attcgctggt gccgccgtca acgacctcga tgatccgggt ggggccggtg atcgagatct
      961 cgtcgatgcc gtcgtcgccg gaaacgacca gagcccgttc ggtgccgaga ccggccagcg
     1021 cctcggcgat cgactcctgg taacggcgat ccgagacgcc catcaactgg cgcctggcac
     1081 cggccggatt ggtcagtggt cccaggaagt tgaagatcgt tcgcaccgcc agcgccttgc
     1141 ggaccggcac cacatgcgcc atcgccttgt ggtgtctggg cgcgaacatg aaaccgaagc
     1201 cggtctcctc gatcgatgcc gccacctttt ccggcgtgat ctcgatgtcg acgccaagtg
     1261 cctcgagcag gtcagccgaa ccgctgaggc tggtgctgga acgattgccg tgcttggcca
     1321 ccggacagcc ggccccggcc gcaaccaggg cggcggtcgt ggagacgttg aaagtcgagg
     1381 ggcctcctcc ggttccggcc gtgtccacca gatcaccgga aacctcgacc ggctcggcca
     1441 gggaccgcat ggtccgggcc agcccgatca actcgggaac agtctcgccc ttggttctga
     1501 gggcgatcag gaaggcagcg gtctgcacct cgtcggagcg gccttccatg atctcggtca
     1561 gcacggccga ggcatggtcc gtggtcaggt gctggccgga ggcggccgcg tcgatcgccc
     1621 tggtcaggat gtcgttaggc aactggtttc ccttcgttgt ccggaattcc ggcggcggcc
     1681 tgctccaggg aaagctcg
//
LOCUS       DAOQMF010000483         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53346_length_1690_cov_3.777982, whole genome shotgun sequence.
ACCESSION   DAOQMF010000483 DAOQMF010000000
VERSION     DAOQMF010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53346_length_1690_cov_3.777982"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..>1690)
                     /locus_tag="PLV77_08085"
     CDS             complement(146..>1690)
                     /locus_tag="PLV77_08085"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNC15844.1"
                     /translation="VMLALLVFSAPASATTFCVPGFFDACPDNGTNQARTDLGDAMTD
                     DGSDGIPDKVMIDSGTITSTGSIVAGGIDPLEVTGAGRDKTFLTSSSTGNISVLRINN
                     RKGTKMSDLTIVVPASFPDEFGNGSAAQIGDSELTRVDVEVRNSDSSAFASMVGSNLI
                     RDSHVYAAEGSKVRWAFVTGGAPGKSTIIGARVEDPVYGFVADNPDTSIEVKASRVIG
                     PTAYGAWAGSGGQISLENTLIATYGGGALTADPENGESDPTIVTAVSSTMVNTGTSTS
                     PGIQVDIPNSITAGDAFVTVTDSIIRGYGATWDLFVPIGPGFAAANLNVVYSNFPPVA
                     APGSAGNVNVGSPTNINQDPLFVSPQDFRLKPGSPSVDAGNPASTLTTDIDGSPRPRD
                     GDGNRTSINDQGAFEFQPTCATAPALCPTVSKVKFRFRAGKGGSLRFLLSLAAKVRAV
                     FTPVPAHRKPKRKVVKITRKLKAGQVKIKLGKHRLMPDRYKLKITAYDKTGHSAKPLI
                     KKVTVK"
BASE COUNT          277 a          517 c          570 g          326 t
ORIGIN      
        1 atgacgcttc gtatgtggcg atcgccgaac ggctgggacg gaaggtggtt tctgccgacc
       61 aggacctgat cgggccaggg ctggcggttg acctggctac tgcggtcggg gctggctgaa
      121 gaacgctccc ggcccgggcg cgggattact tgacggtgac cttcttgatc aggggcttgg
      181 cgctgtggcc ggttttgtcg taggcggtga tcttcagctt gtagcggtcc ggcatgagcc
      241 ggtgcttgcc gagcttgatc ttcacctggc cggccttgag cttgcgggtg atcttcacca
      301 ccttgcgctt cggcttccgg tgcgcgggga ccggcgtgaa gaccgccctc accttggccg
      361 ccagggagag gaggaagcgg agcgagccgc ccttgccggc ccggaacctg aacttcacct
      421 tcgagacggt cgggcaaagt gccggggccg tcgcgcaggt gggctgaaac tcgaaagctc
      481 cttgatcgtt gatcgaggtc cggttgccgt caccgtcccg ggggcggggg ctgccgtcga
      541 tgtcggtggt cagggtcgag gccggattgc ccgcatcaac cgaaggtgag cccggcttca
      601 ggcgaaagtc ctgggggctg acgaaaagcg gatcctggtt gatgtttgtc gggctcccga
      661 cgtttacgtt tccggccgag cccggggcgg cgaccggggg gaagttcgaa tagacgacgt
      721 tgaggttcgc cgcggcaaag cccggcccga tcgggacgaa cagatcccag gtcgccccgt
      781 agccacggat gatcgagtcg gtgactgtca cgaaggcgtc accggcggta atcgaattcg
      841 gaatgtcgac ttgaatcccg ggagatgtcg aggtgccggt gttgaccatc gtcgacgaga
      901 ccgcggtcac gatggtcggg tcggattcgc cgttctccgg gtccgccgtc aaagcgcctc
      961 ccccgtaggt cgcgatcagg gtgttctcga gactgatttg ccctcccgac ccggcccagg
     1021 cgccataagc ggtcgggccg atcacccggc ttgccttcac ctcgatcgag gtatcggggt
     1081 tgtcggctac gaacccgtag accgggtctt caaccctggc tccgatgatc gttgacttgc
     1141 ccggtgcccc gccggtgacg aatgcccatc gcaccttcga cccttcggcc gcgtacacat
     1201 gtgaatccct gatcaggttg ctcccgacca tcgaggcgaa ggcgctcgaa tcggagttcc
     1261 tgacctccac gtcaaccctg gtcagctccg aatccccgat ctgggcggcc gaaccgtttc
     1321 caaattcgtc agggaacgaa gccggtacga cgatggtcag atcggacatc ttcgtgccct
     1381 tgcggttgtt gatccgcaac acgctgatgt tcccggtcga gctcgaggtg aggaaggtct
     1441 tgtccctgcc cgccccggtc acctcaagcg gatcgatgcc gccggcgacg atcgagccgg
     1501 tggaggtgat cgtccccgag tcgatcatca ccttgtccgg gattccatcg ctgccgtcat
     1561 cggtcatcgc atcgcccagg tccgtccggg cctggttggt gccgttgtcg ggacaggcat
     1621 cgaaaaagcc cggcacgcag aaggtcgtgg ccgaagccgg agcggagaaa acgagcagcg
     1681 cgagcatcac
//
LOCUS       DAOQMF010000484         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53356_length_1690_cov_3.327829, whole genome shotgun sequence.
ACCESSION   DAOQMF010000484 DAOQMF010000000
VERSION     DAOQMF010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53356_length_1690_cov_3.327829"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..106)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            213..1241
                     /locus_tag="PLV77_08090"
     CDS             213..1241
                     /locus_tag="PLV77_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNC15845.1"
                     /translation="MEAAMPNLPRLRRARESELLSPDSVEPEVLAPRVEEITSGGLRW
                     INIESPGGEERAWLEENFDFHALDIEDVLSHNQRPKIDEYPDYLFLVVHVPVFDRKAK
                     RLVAGEIDIFVGPDFMVTLPNRGIPPVEYLFERCRADDTLRHKLFARGSGYLMYRLVD
                     DSFDYCFPMLRKIGNKLDALEEEIFEGRSEDVVRDLSNAKQEIINFRKVIRPQRPVLR
                     DLENVKSRYLASDLDLEIYFDDIVDAHERIWDMLENYKEVVVALEETNESIISHKVND
                     ILRVLTSISVIVLPLTLIASIFGMNVGLPGGGDPATSTDFHFFVVVGIMLVILVGMVA
                     YFRKRDWL"
     gene            1354..>1690
                     /locus_tag="PLV77_08095"
     CDS             1354..>1690
                     /locus_tag="PLV77_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNC15846.1"
                     /translation="MAENRIWAPWRLKYVKDADKNNEDQCVFCAKPALGDDETALVVH
                     RGENAFVILNLYPYTNGHLMVAPFEHIGRIQEIPEETTTEMMALAKVAMNRLEDVYGP
                     QGFNVGFNQG"
BASE COUNT          334 a          531 c          487 g          338 t
ORIGIN      
        1 tcccggctac ttgagccgct ggatttggca ccttctcgaa agctctcgct tccgctggtt
       61 gccggggttt cgtagggcca tgtccctcca cccctctgga tgtggcgctt atgtctggcg
      121 acaggttacc agcatgcata aacatcccgt ttacgcgggg gtttgactgc tggtctaggg
      181 ttgtttcatg cccgcgaagc gaattgacat gaatggaggc ggcgatgccg aacctccctc
      241 gtcttcgccg tgcgcgcgaa tctgagctgc tgagcccgga ttcagtcgaa cccgaggtcc
      301 tcgccccgcg ggtcgaggag atcacctcgg gcggtttgcg ctggatcaac atcgaaagcc
      361 ctggcgggga ggaacgcgcc tggctggagg agaacttcga tttccacgcc ctcgacatcg
      421 aggacgtgct ttcgcataac cagcgaccaa agatcgacga atacccggac tacctcttcc
      481 tcgtcgtcca tgtaccggtt ttcgaccgca aggccaagcg cctggtcgcc ggcgaaatcg
      541 acatcttcgt cggacccgat ttcatggtca ccctgccgaa ccgcgggatc ccgccggtcg
      601 agtacctctt cgagcgctgc cgggccgatg acaccctccg ccataaactc ttcgcccgcg
      661 gttccggtta cctgatgtac cgcctggtcg atgacagctt cgattactgc ttcccgatgc
      721 tgcgaaagat cggcaacaaa ctcgatgcgc tcgaggaaga gattttcgag ggccgctccg
      781 aggatgtggt tcgcgacctc tcgaacgcca agcaggagat catcaacttc cgcaaggtga
      841 tccggccgca gcgaccggtt ctgcgtgacc tcgagaacgt caagtcccgc tacctggcca
      901 gtgatctcga cctcgagatc tacttcgacg acatcgtcga cgcccacgaa cggatctggg
      961 acatgctcga gaactataag gaggtcgtgg tcgcgctcga ggagaccaac gaatcgatca
     1021 tctcccacaa ggtcaatgac atcctgcggg tgctcacctc gatctcggtg atcgtcctgc
     1081 ccttgaccct gatcgcctcg atcttcggca tgaacgtagg cctgcccggt ggcggcgacc
     1141 cggccaccag taccgatttc cacttcttcg tggtggtggg gatcatgctg gtgatcctgg
     1201 tcggcatggt cgcctacttc cgcaagcgag actggctctg aataagtagt cggcgagctt
     1261 cgccccgcct ccaagttcca cccgtccgat cacagagaaa gcgaatagat gatcggactc
     1321 cgactcgcgt tggaccaata ggatggggcg ccgatggcag agaaccggat ttgggcaccc
     1381 tggcgcctca agtatgtgaa ggacgccgac aagaacaacg aggaccagtg cgttttctgc
     1441 gccaagccgg cgctcggtga cgatgaaacc gcgctggtcg tccaccgcgg cgagaacgcc
     1501 ttcgtgattc tcaacctcta cccgtacacg aacgggcacc tgatggtcgc ccccttcgaa
     1561 cacatcggcc gcattcagga gatccccgaa gagacgacca ccgagatgat ggcccttgcc
     1621 aaggtggcga tgaaccggct cgaggatgtc tacggaccgc agggattcaa cgtcggtttc
     1681 aaccagggcc
//
LOCUS       DAOQMF010000485         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53373_length_1690_cov_2.453823, whole genome shotgun sequence.
ACCESSION   DAOQMF010000485 DAOQMF010000000
VERSION     DAOQMF010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53373_length_1690_cov_2.453823"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1164)
                     /locus_tag="PLV77_08100"
     CDS             complement(4..1164)
                     /locus_tag="PLV77_08100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNC15847.1"
                     /translation="MSRAAHLANRAALAGCGLLLAILLAAFLLPSSPAAAGGEPSSSE
                     SGATASIVNGKATTIGQWPWQVALTVSSRIAPRASTSWRFFCGGSVLAPRLVITAGHC
                     VADLNRGQIRKIEIVSGRTRLNSNRGQVARVTGLRMPVNASGKRRFRGLAGAADWDVA
                     LLTLASPLSAEPIKLAGPDEAAAWSPGQVAWTTGWGITKAFSQRVPARLQVARQVLMG
                     NGLCHRADGVAYKSRRMICMGAPRAHASACNGDSGGPLVVETGAGYRLVGLTSYGDGA
                     CRGFVPSVDTRVSGRQIRNWVARTAMNLTGRNVVGSGGTPRPAPDWCKVPDVFRLRQS
                     QAKARLEAAGCRLGRVRTDYYSVGQAGRIVGSRLAPGWLAPPGFKVGVWVAP"
     gene            1328..>1690
                     /locus_tag="PLV77_08105"
     CDS             1328..>1690
                     /locus_tag="PLV77_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC15848.1"
                     /translation="MAFGKKKRESAGAELEAARARKQAERPRKGGAPIIEMRDVSKVY
                     PGGHLAVDRVSLAINRGEFVFLVGPTGCGKSTLIKMLIRELEPTDGTISIAGRDINQL
                     SEKKIPQLRRNIGTVFQDF"
BASE COUNT          307 a          559 c          578 g          246 t
ORIGIN      
        1 cgatcatggg gcaacccaaa ctcccacctt gaagccgggc ggggccagcc atccgggtgc
       61 gaggcggctg ccgacgatcc ggccggcctg gccgacgctg taatagtcgg tcctgacccg
      121 gccgaggcgg caacccgcgg cctcgagcct ggccttcgcc tggctctgcc tgagccggaa
      181 gacatccggc accttgcacc agtccggagc gggcctggga gtgccaccgc tgccgaccac
      241 gttgcggccg gtcaggttca tcgcggtccg tgcgacccag ttgcggatct gcctgccgga
      301 gacccttgtg tcaaccgagg ggacaaatcc gcggcaggca ccatcgccat aactggtcag
      361 gccgaccagc cggtaaccgg caccggtctc gaccacgaga ggtccgccac tgtctccatt
      421 gcaggccgag gcatgggccc ggggtgctcc catgcagatc atccggcggg acttgtacgc
      481 gacgccgtct gcccggtggc agaggccgtt gcccatcagg acctgccggg cgacctggag
      541 cctggccgga acccgctgcg agaaggcctt ggtgattccc cagcctgtgg tccaggcgac
      601 ctgtcccggg gaccaggccg ccgcctcgtc cggaccggcc agcttgatcg gctcggccga
      661 aaggggggaa gcgagggtca gcagggcgac gtcccagtcg gccgcgccgg caagaccgcg
      721 gaagcgccgc ttgccggagg cgttcaccgg catgcggagc ccggtcaccc tggccacctg
      781 gccccggttc gaattgagac gggtgcggcc ggaaacgatc tcgatcttgc ggatctgccc
      841 ccggttgagg tcggcgacgc agtgaccggc agtgatcaca agccgcggcg cgagaaccga
      901 gccgccgcaa aagaaacgcc agctggtcga ggcccgcggc gcaatccgcg agctcacggt
      961 cagagcgacc tgccagggcc actggccgat cgtggttgcc ttgccgttga cgatcgaggc
     1021 cgtggcgccg gattcactgc ttgaaggttc gccgccggcc gcggccgggg aggagggcag
     1081 aaggaacgcc gcgagcagga tcgcgaggag gaggccgcaa cctgcgaggg cggcccggtt
     1141 agccaaatgg gcagccctgg acattgcagg aatcctaccg gccgcgctta agatcagcca
     1201 aagtcatata agcgattgct aacgtttcgc taactctgaa gaaatgaacc tggccggggt
     1261 tgccctttgt gggagatccc cgtcagggga aggattaccc cgggccgggg ggaatagggc
     1321 gaaccagatg gctttcggaa agaagaaaag agaatcagca ggcgccgagc tcgaagcggc
     1381 acgcgcccgc aagcaggccg agcgtccgcg caagggtggc gccccgatca tcgagatgcg
     1441 tgacgtcagc aaggtgtacc cgggcggcca ccttgccgtt gaccgggtct cgctggcgat
     1501 caaccgtggc gagttcgtct tcctggtcgg cccgaccggc tgcggcaagt cgaccctgat
     1561 caagatgctg attcgcgagc tcgagcccac cgacggcacg atctcgatcg ccggtcgtga
     1621 catcaaccag ctttccgaga agaagattcc gcagctccgc cgcaacatcg gcaccgtctt
     1681 ccaggacttc
//
LOCUS       DAOQMF010000486         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53484_length_1688_cov_3.028169, whole genome shotgun sequence.
ACCESSION   DAOQMF010000486 DAOQMF010000000
VERSION     DAOQMF010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53484_length_1688_cov_3.028169"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..287)
                     /gene="rpsS"
                     /locus_tag="PLV77_08110"
     CDS             complement(21..287)
                     /gene="rpsS"
                     /locus_tag="PLV77_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNC15849.1"
                     /translation="MGRSTKKGPYVEERLMGRIEAMNEAGNKNMVKTWSRRSTIFPEM
                     VGHTIAVHDGRKHVPVFISESMVGHKLGEFAPTRTFKSHTGGKD"
     gene            complement(291..1136)
                     /gene="rplB"
                     /locus_tag="PLV77_08115"
     CDS             complement(291..1136)
                     /gene="rplB"
                     /locus_tag="PLV77_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNC15850.1"
                     /translation="MAIRKTKPTSPGRRFATYQLREQITATEPEKSLIEGIKKSGGRN
                     NNGRITSRHRGGGAKRRYRKIDFKRTKDGIPAKVATVEYDPNRSAYIALLHYADGEKA
                     YILAPSQIEVGNQVSSGPGSDIRPGNCLALGEMPVGTTVHNVELKPGQGGKLGRSAGT
                     AIQLVAKEGDMVTLRLPSSEMRMVRAECRATIGTISNAEHQNVKIGKAGRNRHKGKRP
                     QSRGIAMNPVDHPHGGGEAHKTPGGHPVTPWGKPTLGYRTRKKGKQSDRYIVRGRRRG
                     KKKGR"
     gene            complement(1160..1453)
                     /gene="rplW"
                     /locus_tag="PLV77_08120"
     CDS             complement(1160..1453)
                     /gene="rplW"
                     /locus_tag="PLV77_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNC15851.1"
                     /translation="MDPRSVIIRPVISEKAYALIAANKYTFRVDDRAHKFEIKDAVEK
                     AFGVEVVKVRTSKVRSKPKRRGLHQGRSRSWKKAIVELAPGDSIELFEGAAVE"
     gene            complement(1453..>1688)
                     /gene="rplD"
                     /locus_tag="PLV77_08125"
     CDS             complement(1453..>1688)
                     /gene="rplD"
                     /locus_tag="PLV77_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNC15852.1"
                     /translation="DKWGAKRSTLVIIQADETGILKSFRNIPRVNVLEVGATGVVDVI
                     GAASIVVSEAALEVLAAQSTEPDRGTGTEKDGE"
BASE COUNT          235 a          546 c          545 g          362 t
ORIGIN      
        1 cctggtggtc ttggcagcca tcagtcctta ccgccggtgt gggacttgaa ggtgcgggtc
       61 ggggcgaact cgcccagctt gtgaccgacc atcgattcgg agatgaagac aggcacgtgc
      121 ttgcgaccgt cgtggacggc gatcgtgtgg ccgaccatct ccgggaagat cgtcgaacga
      181 cgcgaccagg tcttgaccat gttcttgttg ccggcctcgt tcatggcttc gatccggccc
      241 ataagccgct cttccacgta cgggcccttc ttggttgacc tacccacggt ctacctaccc
      301 ttcttctttc cgcgacgacg gccgcgcacg atgtaacggt cggactgctt gcccttcttg
      361 cgggtgcggt acccgagcgt gggcttgccc cagggagtga ccggatggcc accgggggtc
      421 ttgtgtgcct cgccaccgcc atgcgggtgg tcgaccgggt tcatggcgat gccgcgcgac
      481 tgcgggcgct tgcccttgtg ccggttgcgg ccggccttgc cgatcttgac gttctgatgc
      541 tccgcgttcg aaatcgtgcc gatcgtcgcg cggcattccg cacggaccat ccgcatttcc
      601 gaggaaggaa ggcgcagggt gaccatgtca ccctccttcg cgaccagctg gatcgcggtg
      661 ccggcgctgc ggccgagctt gccgccctgg ccgggcttca gctccacgtt gtggacggtg
      721 gtaccgaccg gcatctcgcc gagggcgagg cagttgccgg gacggatgtc cgagcccggg
      781 ccggagctga cctggttgcc gacctcgatc tgcgagggcg ccaggatgta ggccttctcg
      841 ccatccgcgt aatgcagcag ggcgatgtag gcggagcggt tcgggtcgta ctcgacggtc
      901 gcgaccttgg cggggatccc gtccttggtc cgcttgaagt cgatcttccg gtagcggcgc
      961 ttggcccctc cgccacggtg gcgggaggtg atgcggccgt tgttgttgcg gccacccgac
     1021 ttcttgatgc cctcgatcag gctcttctcg ggctcggtcg cggtgatctg ctcacgcagc
     1081 tggtacgtcg cgaagcgacg cccggggctc gttggttttg tcttgcgaat tgccattact
     1141 cagttattcc ttcaagccct tactcgaccg ccgcgccttc gaacagctcg atgctgtcgc
     1201 cgggggcgag ttcgacgatt gccttcttcc aggagcggct gcgaccctga tgcaggccac
     1261 gacgcttcgg ctttgaacgg acctttgagg tccggacctt gaccacttcg actccgaacg
     1321 ccttctcgac ggcgtccttg atctcgaact tgtgggcccg gtcgtccacc cggaaggtgt
     1381 acttgttggc cgcgatcagg gcgtaggcct tttccgagat gaccggacgg atgatcacgc
     1441 tgcggggatc catcactcac cgtccttttc ggtcccggtt ccccggtccg gttcggtcga
     1501 ctgggcggcc agaacctcga gggccgcttc ggaaaccacg atcgaagcgg cgccgatcac
     1561 gtcgaccact cccgttgctc caacctcgag cacattgacc cgcgggatgt tgcggaagct
     1621 cttgaggatg ccggtctcgt cggcctggat gatcaccagg gtcgaacgct tcgcgcccca
     1681 cttgtcca
//
LOCUS       DAOQMF010000487         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53519_length_1687_cov_4.545956, whole genome shotgun sequence.
ACCESSION   DAOQMF010000487 DAOQMF010000000
VERSION     DAOQMF010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53519_length_1687_cov_4.545956"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLV77_08130"
     CDS             <1..920
                     /locus_tag="PLV77_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC15853.1"
                     /translation="ILESTTYPGTTRDLLASTLEAGSRVAGRDFHLAFSPERIDPGRT
                     DFTVRTTPKLVGGLTPACTDKAVALYETICDRVIPLSGPEAAELSKLLENIFRSVNIA
                     LVNELAQLCDRLGVDVWEVIDAASTKPFGFMRFEPGPGMGGHCLPVDPFYLAFKAREH
                     DFYPEFIELAGKINQSQPLFCVNRIERVLNANGKPVKGTKVLILGVSYKAGVGDTRES
                     PAIKIIRLLGELGADVRYHDPHVPALKEAGLTNTPDLDGALADADLSVIITAHPEFDW
                     AEVIGKSDLVFDLRGVTREFDDPGNVTRL"
     gene            947..>1687
                     /locus_tag="PLV77_08135"
     CDS             947..>1687
                     /locus_tag="PLV77_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15854.1"
                     /translation="MIRELKGAASSLIPWRRAPHSHWQTIKSDRATLALAVGTVTIAG
                     LVLAAQYSRLLSRRTHSPGTEGLIDSAPAAAVDTVGVAVEGYSATPNRELILFNLLTG
                     FLGSFSAVRMTTWAIREGWGPFRNVSIGGRHIHHFVPGILIGFGSGIAALLLNGEKAD
                     RRIARSLGVGMGLTFDEAALLLDMQDVYWTRQGLLSVQVTLATGATLGATILTMRILG
                     RGETRQEEAGEIPAPEGQMNTVVPWPHPA"
BASE COUNT          279 a          579 c          545 g          284 t
ORIGIN      
        1 tgatcctcga atcgaccacc taccccggca cgacccgcga cctgctggcg agcacgctcg
       61 aagcaggcag ccgggttgca ggccgggatt ttcacctcgc cttttccccg gaacggatcg
      121 atccgggccg caccgacttc acggtccgca ccaccccgaa actggtcggt ggtctcaccc
      181 ccgcctgtac cgacaaggcg gtggcgcttt acgaaacgat ctgcgaccgc gtgatcccgc
      241 tctcgggccc tgaggctgcg gagctttcga agctgctgga gaacatcttc cgctcggtca
      301 acatcgcgct ggtcaacgaa ctggcacagc tctgcgaccg gctcggcgta gacgtctggg
      361 aggtgatcga tgccgcttcg accaagccct tcggtttcat gcggttcgag ccgggccccg
      421 gcatgggcgg tcactgcctg ccggttgatc ccttctatct cgccttcaag gcacgcgagc
      481 atgacttcta cccggagttc atcgagctcg ccggcaagat caaccagtca cagccgctct
      541 tttgcgtcaa ccggatcgag cgggttctca atgcgaacgg caagccggtc aaaggcacca
      601 aggtcctgat cctcggcgtc agttacaagg ccggggtcgg cgacacgcgc gagtcgcccg
      661 cgatcaagat catccggctg ctgggtgagc tgggcgcgga cgtgcgctac cacgacccgc
      721 atgtgccggc cttgaaggag gccggactga ccaatacccc cgacctcgac ggcgccctcg
      781 ccgacgccga cctcagcgtg atcatcaccg cccacccgga attcgactgg gcggaggtaa
      841 tcggaaagag tgacctggtc tttgacctgc gcggggtcac ccgggagttc gatgatcccg
      901 gcaacgtgac gaggctctga ccgggccggg aagaaggagc gcgggcttga ttcgggaact
      961 gaagggcgcc gcgtcgagcc tgatcccctg gcgacgggcg cctcactctc actggcagac
     1021 gatcaagagc gaccgggcga ccctcgccct ggccgtgggc acggtgacga tcgccggact
     1081 ggttctcgcc gcgcagtact cgcggctgct ttcccgccgc acccattcac ccgggaccga
     1141 gggtctgatc gattcggccc cggcggccgc ggtcgacacg gtcggggtcg cggtcgaggg
     1201 gtactcggcc accccgaacc gggagctgat cctcttcaac ctgctgaccg gttttctcgg
     1261 cagcttctcc gcggtccgga tgaccacctg ggcgatccgg gaaggctggg gccctttccg
     1321 caacgtcagc atcggcggcc gccacattca ccatttcgtg ccggggatcc tgatcgggtt
     1381 cggctccggc atcgctgccc tgctcctgaa cggcgagaag gccgaccggc gaatcgcccg
     1441 cagcctcggg gtcgggatgg gcctcacctt tgacgaggcg gccctgctgc tcgacatgca
     1501 ggacgtgtac tggaccaggc aggggctgct ttcggtacag gtgaccctgg ccaccggggc
     1561 taccctcggg gcgacgatcc tgaccatgcg gatcctcggc cgcggcgaaa cccgccagga
     1621 ggaggcgggt gagatcccgg cccccgaggg acagatgaac acggttgtac cgtggcctca
     1681 cccggct
//
LOCUS       DAOQMF010000488         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53531_length_1687_cov_3.838848, whole genome shotgun sequence.
ACCESSION   DAOQMF010000488 DAOQMF010000000
VERSION     DAOQMF010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53531_length_1687_cov_3.838848"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLV77_08140"
     CDS             complement(<1..218)
                     /locus_tag="PLV77_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15855.1"
                     /translation="MSRGHLAAVLVTLTCLLAIPASASAKEATAAYTFDPGQVAFGDV
                     PVGSTASQLITLTNTGGSPALIRGAFAG"
     gene            374..865
                     /locus_tag="PLV77_08145"
     CDS             374..865
                     /locus_tag="PLV77_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNC15856.1"
                     /translation="MSDSESPVVLTGTMNRLNGTEQDLGEYLGQVTLVVNTASKCGLT
                     PQFEALQAVYSDKKDEGFTVLGFPANDFMEQEPGSADEIAEFCQINYGVEFPMFEKST
                     VVGDEANPLFAELAAQSEQPEWNFAKYLIDREGRLVRKFPAPTTPDDPEVIEAIEAEL
                     ASE"
     gene            complement(875..>1687)
                     /locus_tag="PLV77_08150"
     CDS             complement(875..>1687)
                     /locus_tag="PLV77_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HNC15857.1"
                     /translation="AVIGVHDADPDTAVPDVAPIAFGGNRRSAAEESRTILANATHGT
                     LGSLSEDGTPWASLVTFGVMDDGSPVFCLSTLAEHARNLENSRQASLMVSDPFHTGEI
                     LAGGRVTLAGKVKRSDEHPDETSARAAHLEAVPSAAMYADFNDFAFWILEVDRVRWVG
                     GYGRMDSASAEEYSQAEPDPVQPQVPDAIAHLNADHPDALLLMARAFTKYQDATEAKC
                     ERADRYGLDLQLTTPEGDKAARVSFTDRIDKPGGLRAATVDLARRSRQKLGS"
BASE COUNT          292 a          525 c          583 g          287 t
ORIGIN      
        1 tttccggcaa aggcccccct gatcagggcc gggctcccac cggtattggt cagagtgatc
       61 aactgtgacg cggtggagcc gaccggaacg tcgccgaagg cgacctggcc ggggtcgaat
      121 gtgtaggccg ccgtggcctc tttcgccgaa gcggatgccg ggatggcgag caggcaggtc
      181 aacgtcacca gaactgcggc cagatgaccc cttgacatcc cttaccccca attgctcgtt
      241 acctgaaagg aagatcccct ctgccgacca tattcctttt ttcggtccgg cgagccagcc
      301 tagttcttcc cggttatctg ggaaggatgg aaaggctgaa agttgccggt ccggagcggg
      361 tacgatcccg ctcatgagcg atagcgagag tccggtcgtc ctgaccggca ccatgaaccg
      421 gctgaacggc accgaacagg atctggggga gtacctgggt caggtgaccc tggtcgtcaa
      481 taccgccagc aagtgcgggc tgaccccgca gttcgaggcc ctgcaggcgg tctattccga
      541 caagaaggac gaggggttca cggtgctcgg tttcccggcc aacgacttca tggagcagga
      601 acccggcagt gccgacgaga tcgccgagtt ctgccagatc aactacgggg tcgagttccc
      661 gatgttcgag aagtcgaccg tggtcgggga cgaggcgaac ccgctctttg ccgaactggc
      721 cgcacagtcc gaacagccgg aatggaactt cgccaagtac ctgatcgacc gcgaaggccg
      781 gctggtcagg aagtttccgg caccgaccac cccggacgac ccggaagtga tcgaggcgat
      841 cgaagccgag ctggcctccg aatagggggc cggcctagct gccgagcttc tggcgcgagc
      901 gccgcgcgag gtcgacggtc gccgcgcgca ggccgccggg cttgtcgatc cggtcggtga
      961 aactgacccg ggccgccttg tcgccctcgg gagtggtgag ctgaaggtcc agtccgtagc
     1021 ggtcggcccg ttcgcacttc gcctcggtcg cgtcctggta tttggtgaag gccctggcca
     1081 tcagcaggag cgcatcgggg tgatcggcgt tcagatgggc gatcgcatcg ggcacctggg
     1141 gctggaccgg atccggctcg gcctggctgt actcctcggc cgacgctgaa tccatgcggc
     1201 cgtagccacc gacccagcgc acccggtcga cctcgaggat ccagaaggcg aagtcattga
     1261 agtccgcgta catggcggcg gaagggacgg cttcgaggtg tgcggcccgg gccgaagttt
     1321 cgtcggggtg ctcgtccgaa cgcttgacct tgccagccag ggttacccgg ccaccggcca
     1381 ggatctcgcc cgtgtgaaac gggtcgctga ccatcagcga agcctgccgg ctgttctcga
     1441 ggttgcgggc gtgctcggcc agggtcgaga ggcagaagac cgggctgccg tcatccatga
     1501 ccccgaaggt gaccagcgaa gcccaggggg tgccgtcctc gctgagcgat ccgagcgtgc
     1561 cgtgagttgc gttggcgagg atcgttcgcg actcctcggc ggcgctgcgg cggttgcctc
     1621 cgaaggcgat cggggcgacg tccggaaccg cggtgtccgg gtcagcgtcg tgaactccga
     1681 tgacggc
//
LOCUS       DAOQMF010000489         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53553_length_1687_cov_2.780025, whole genome shotgun sequence.
ACCESSION   DAOQMF010000489 DAOQMF010000000
VERSION     DAOQMF010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53553_length_1687_cov_2.780025"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..373
                     /locus_tag="PLV77_08155"
     CDS             20..373
                     /locus_tag="PLV77_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC15858.1"
                     /translation="MEAAKSQTGGSAGHALRAGPLEVRLDQRTVLADGRGIILTVREF
                     HLLAVLASRPEQVVSREEVYDLVWGGAMPENDRSVDVYVSKLRHKLETALPHWSFIQT
                     HIGFGYCFSPRPLNP"
     gene            508..1602
                     /gene="pstS"
                     /locus_tag="PLV77_08160"
     CDS             508..1602
                     /gene="pstS"
                     /locus_tag="PLV77_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNC15859.1"
                     /translation="MNLKKWLIAIPLTLALALGVAACGSSDSDSGSSGSSGDAVSGEI
                     AGAGASSQEAAMAAWVAGFQENNPDANIAYDPVGSGGGREQFIAGGTDFGGTDTPMDE
                     TELADVAKRCKPGEYIEIPVYISPIAVPYNVEGVDDLNLSASTLARIFAGDITKWNDQ
                     AIAAENPDASLPDQDIVVVHRSDESGTTENFTDYLHQAAPKDWTDEPDGVWPLQGGEA
                     ANGTSGVISAVKSGSGTIGYADLSQVGDLGTVNVKVGSDFVAPSPEAAVKIFDESKRI
                     GGEGKYIFAYELNRTSTAQGLYPISLVSYEMACTTYDSADTAKLVKAFYSYIISDEGQ
                     QAAASNAGSAPIPASLSKQITPAVEAIQTS"
BASE COUNT          332 a          569 c          523 g          263 t
ORIGIN      
        1 gatctgagcc gggccgagcg tggaagcagc aaagtcccag acgggcggtt ccgccggcca
       61 cgcgctgagg gccgggcccc tggaagtccg gctcgatcag cggaccgtcc tcgccgacgg
      121 caggggaatc atcctcacgg tccgggagtt ccacctgctc gcggtcctgg ccagccggcc
      181 ggaacaggtc gtgagcaggg aagaggtcta cgacctggtc tggggcggcg cgatgcccga
      241 aaacgaccgg tcggttgacg tctacgtctc gaaactgcgc cacaagcttg aaaccgcctt
      301 gcctcactgg agcttcatcc agacccacat cggctttggc tactgctttt cgccgcgacc
      361 gctgaacccc tgactcgcgg caagtgacgg gcttttcacc tcttttcaca actttcacaa
      421 ggaagtaaca ccgcgcgaaa tctccatcta ttgactgaag ttcattactg cgcaacccct
      481 cgaaagaaat ccaaactgga ggaaaccttg aacttgaaga agtggctaat cgcgatcccc
      541 ctcaccctgg ccctggcgct gggtgtggcc gcctgcggaa gcagcgacag cgacagtggc
      601 agcagcgggt ccagcggcga cgcggtctcc ggcgagatcg ccggcgccgg cgccagctca
      661 caggaagccg ccatggccgc ctgggtcgcc ggcttccagg agaacaatcc tgacgccaac
      721 atcgcctacg acccggtcgg ttccggcggc ggccgcgagc agttcatcgc cggtggcacc
      781 gacttcggcg gcaccgacac cccgatggac gagaccgaac tggccgatgt cgccaagcgc
      841 tgcaagccgg gcgagtacat cgagatcccg gtctacatct cgccgatcgc ggttccgtac
      901 aacgtcgagg gggtcgatga cctgaacctc tcggccagca ccctggcccg gatcttcgct
      961 ggtgacatca ccaagtggaa cgatcaggcg atcgcggcgg agaacccgga cgcgagtctg
     1021 cctgaccagg acatcgtggt cgtccatcgc tcggatgagt cgggcacgac cgagaacttc
     1081 accgactacc tgcaccaggc ggctccgaag gactggaccg acgagcccga cggggtctgg
     1141 ccgctccagg gcggcgaggc tgccaacggc acttccggcg tgatcagcgc cgtcaagtcc
     1201 ggcagcggca cgatcggtta cgccgatctg agccaggtcg gtgacctggg caccgtcaac
     1261 gtcaaggtcg gcagcgactt cgtggctccc tcacccgaag cagctgtgaa gatcttcgac
     1321 gagtcgaagc ggatcggtgg cgaaggcaag tacatcttcg cgtacgagct caaccgcacc
     1381 tcgaccgccc agggtctcta cccgatctcg ctggtgtcat acgagatggc ctgcaccacc
     1441 tacgactcgg ctgacacggc caagctcgtc aaggccttct acagctacat catcagcgac
     1501 gaaggccagc aggctgcggc ctcgaacgcc gggtcggctc cgatcccggc atccctcagc
     1561 aagcagatca ctcccgccgt ggaggcgatc cagaccagct gagctgaagc aagccggcag
     1621 cgagaacccc gcggcggagg cacaggccgc cgcggggtga tcgcagtcag gctcaccagc
     1681 cgttttt
//
LOCUS       DAOQMF010000490         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53752_length_1684_cov_3.192756, whole genome shotgun sequence.
ACCESSION   DAOQMF010000490 DAOQMF010000000
VERSION     DAOQMF010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53752_length_1684_cov_3.192756"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PLV77_08165"
     CDS             complement(<1..894)
                     /locus_tag="PLV77_08165"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNC15860.1"
                     /translation="MSDYATDKNTEVTMEEIVALAKRRGFVFPSSEIYGGLGSTYDYG
                     HYGVLLKNNVKNEWWKSMLVERDDIVALDSAILQHPKVWEASGHLAGFTDPLVQCLGE
                     CKRRWREDHLREANVEKGGNYDDPIVCPECGGELSPPRNFNLMFETHMGPVADDGNAV
                     YLRPETAQGIFINFKNVLNFARKKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEME
                     FFVPPADAQEWWEKWVEIRHNWYLELGIRPDFLMIRPHEADELSHYSSGTSDVEYLFP
                     IGWSELEGIANRGNFDLTQHQE"
     gene            982..1347
                     /locus_tag="PLV77_08170"
     CDS             982..1347
                     /locus_tag="PLV77_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC15861.1"
                     /translation="MPIYEYRCENGHLFEVMQSMSEDALTECIECGAPVQKVLHSPAI
                     HFKGSGFHNTDYGTRKSNKAKAAAESAKKDSGSSSSSSGSSDSGSSSSGSSDSGSGSS
                     SSSSSDSGSKASSGSSNSD"
     gene            1391..1675
                     /locus_tag="PLV77_08175"
     CDS             1391..1675
                     /locus_tag="PLV77_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNC15862.1"
                     /translation="MDRIGIRELRADLSNQVKRAAAGEAVVVTIDGEPKALLTPFVAE
                     SGEMTLEELIRTGRVIPGPRVNEPRPPRRQGIKGGRPTKDVLDEIREDKI"
BASE COUNT          321 a          486 c          547 g          330 t
ORIGIN      
        1 ctcctgatgc tgggtcaggt cgaagttgcc gcgattggcg atgccttcaa gctccgacca
       61 gccgatcggg aagaggtact cgacgtcgga ggttccggac gagtagtgcg agagctcgtc
      121 cgcctcatgc gggcggatca tcaggaagtc ggggcggatg ccgagctcca ggtaccagtt
      181 gtgacggatc tcgacccact tctcccacca ctcctgggcg tcggccggcg gcacgaagaa
      241 ctccatctcc atctgctcga actcgcgggt ccggaagatg aagttgccgg gggtgatctc
      301 gttgcggaag gacttgccga cctgggcgat cccgaaaggc ggcttcttgc gggcgaagtt
      361 gaggacgttc ttgaagttga tgaagatgcc ctgcgcggtc tcgggccgca ggtacaccgc
      421 gttgccgtcg tcggcgacgg ggcccatgtg ggtttcgaac atcaggttga agttgcgggg
      481 aggggagagc tcgccaccgc attccgggca gacgatcggg tcgtcgtagt taccgccctt
      541 ttccacgttg gcctcgcgca ggtggtcttc gcgccagcgg cgcttgcact cgccgaggca
      601 ctggaccagc gggtcggtga agccggccag gtgcccggag gcctcccaga ccttggggtg
      661 ctgcaggatc gccgagtcga gggcgacgat gtcatcgcgc tcgaccagca tcgacttcca
      721 ccattcgttc ttgacgttgt tcttgagcag aactccgtaa tggccgtagt cgtaggtcga
      781 accgaggccg ccgtagatct ccgaagaagg gaagacgaaa ccgcgccgct tggccagcgc
      841 aacgatctct tccatggtca cttcggtgtt cttgtcggtg gcgtagtcgc tcatgtccta
      901 aaggttatgg acaatcgtcc cgccaagccg ggacgggtat cggggcggaa acgggggcgc
      961 gctgcgataa tcagccgcgc tatgccgatt tacgaatacc gctgtgaaaa cgggcacctc
     1021 ttcgaggtca tgcagtcgat gagcgaggat gccctgaccg aatgcatcga atgcggcgcc
     1081 cccgtccaga aggtgctgca ttcaccggcg atccacttca agggctccgg tttccacaac
     1141 accgactacg gcacccgcaa gtccaacaag gcgaaggccg cagccgaatc agcgaagaag
     1201 gacagcggct cgagctccag ctcgtcgggc tcctccgact cgggatcttc gagttccggc
     1261 tcatccgatt ccggttccgg gtctagctcg tcgagctctt cggactccgg ttcaaaagcc
     1321 tcgtcgggtt cttcaaactc cgactgagca aggtcggttt ttcgggtgta ccgtcgtgta
     1381 cactcgctat atggatcgga ttggtatcag ggaactccgc gcggacttga gcaatcaggt
     1441 gaagcgtgct gccgccggtg aagctgtggt cgtcacgatc gatggagagc cgaaagccct
     1501 tctgacaccg ttcgtcgcgg agtctggtga gatgactctg gaagagctga tcaggaccgg
     1561 tcgggtaatt cccggaccga gagtcaacga gcccagacca cctcgacggc aagggatcaa
     1621 aggagggcgt ccgacgaagg atgttctgga cgagatccgc gaggacaaga tctgatgttg
     1681 tgct
//
LOCUS       DAOQMF010000491         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_53974_length_1681_cov_2.303813, whole genome shotgun sequence.
ACCESSION   DAOQMF010000491 DAOQMF010000000
VERSION     DAOQMF010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53974_length_1681_cov_2.303813"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLV77_08180"
     CDS             complement(<1..611)
                     /locus_tag="PLV77_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15863.1"
                     /translation="MLAALLALAAAPALALADGKPDLSFGKKGTSAFDPDPLSLQSPS
                     DMVTDSKGRIVMGGTTFNGPGGNMAWFTRLNRNGSVDSTFGTGGFTTFPVPAQTYIAT
                     VAVDSEGRIVAAGNYDLPMNRDIWVLRLKSDGHLDSGFGIGGGVGFDFGSFDLGYAAV
                     VGKDGLIYVAASTGDGDSDKATVLRYTSDGDPDSTWGTGGAVS"
     gene            761..1387
                     /locus_tag="PLV77_08185"
     CDS             761..1387
                     /locus_tag="PLV77_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNC15864.1"
                     /translation="MEVDLNFYLQSIVAVVVITDPLTRGIFFRQLTANEPERRGEFVR
                     RITIVVAVTLFGAALVGRELLDVMGINLGAFGVAGGLVVTLMGFEMLFGGEPSRAQGG
                     EQSREQPESFDGSVVVPYAIPFIAGPGAITTVITIAASAENGEGTIVALVAVAVAVIL
                     LPIGHLLVAKHVNLSDQAESVVTKIGGLLITTIGIQLMLSGIQRFFEG"
     gene            1460..>1681
                     /locus_tag="PLV77_08190"
     CDS             1460..>1681
                     /locus_tag="PLV77_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15865.1"
                     /translation="MKRGSGETMRDARPAAGQGGRKPSPALVVAIIALIVALGGTSYA
                     AFKLPKNSVGTKQLKKNAVTVKKLRKNSVN"
BASE COUNT          310 a          522 c          554 g          295 t
ORIGIN      
        1 aatgatacgg caccaccggt tccccaggtg gaatcgggat ccccatccga tgtgtagcgg
       61 agcaccgtgg ccttgtccga gtcgccgtca ccggtcgaag cggccacgta gatcaagccg
      121 tccttgccga ccaccgcggc gtaaccgagg tcgaaactgc caaagtcgaa accgactccg
      181 ccgccgatgc cgaagcctga gtcgaggtgc ccgtccgatt tcagacgcag cacccagatg
      241 tcacgattca tcggaaggtc atagtttccc gcggccacga tcctgccctc ggaatcgacc
      301 gcgaccgttg cgatgtacgt ctgcgctgga accggaaacg ttgtgaaacc gccggttccg
      361 aaggtcgagt cgacgctgcc gttccggttc agccgggtga accaggccat gttcccgccc
      421 ggtccgttga aggtggtccc gcccataacg atccgaccct tcgagtcggt gaccatgtcc
      481 gaaggactct ggagcgaaag cgggtcggga tcgaacgcag aggtcccctt cttgccgaag
      541 gacaggtcgg gcttcccatc cgcgagcgcg agggcgggcg ctgccgcaag agccagaagc
      601 gcagccagca acgcaatgac cggaaggcac atgcgccact gacctgaatt gggaacctgc
      661 cgttccattc tccacctcac ttgaactgtc cccaacctat ccccgaccca cgcggatggc
      721 aacccgggcg accgggtcgc tctttaccct gcgggcaggg atggaggtcg atctcaactt
      781 ctacctgcag tcgatcgtcg cggtggtggt gatcaccgat cccctgacgc gggggatctt
      841 ctttcgccag ctgaccgcga acgagcccga gcgccggggc gagttcgtcc ggcgaatcac
      901 gatcgtggtc gccgtgacac ttttcggagc ggcgctggtc ggccgtgagc tgcttgacgt
      961 aatggggatc aatctcgggg ccttcggcgt cgccggcggg ctggtcgtga ccctgatggg
     1021 tttcgagatg ctcttcggcg gtgaaccgag ccgggctcag ggaggcgaac aatcccgcga
     1081 gcagcccgag agcttcgacg gcagcgtcgt cgtcccctac gcgattccgt tcatcgccgg
     1141 accgggcgcg atcaccaccg tgatcacgat cgcggcttcg gccgaaaacg gcgagggtac
     1201 gatcgtcgcc ctggtcgcgg tcgcggtggc ggtgatcctc ctgccgatcg gccacctgct
     1261 tgtcgcgaag cacgtgaacc tctccgacca ggcggagtca gtcgtgacca agatcggcgg
     1321 cctgctgatc accacgatcg ggatccagtt gatgctcagc ggcatccagc gctttttcga
     1381 gggttgagtt tcggggagtc cgggttgttt gcgccggcgc ttgccgtggc ctggcgcgga
     1441 gggtaggctg gaagctctgt tgaagcgagg atcgggggag accatgcggg acgcaagacc
     1501 ggcagccggg cagggcggca ggaaaccgtc accggcgctg gtggtggcga tcatcgccct
     1561 gatcgttgcg ctgggcggaa ccagttatgc ggcgttcaag ctgccgaaga attcggtcgg
     1621 aacgaaacag ctgaagaaga acgcggtgac ggtcaagaaa ctgaggaaga actcggtcaa
     1681 c
//
LOCUS       DAOQMF010000492         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54011_length_1680_cov_3.726154, whole genome shotgun sequence.
ACCESSION   DAOQMF010000492 DAOQMF010000000
VERSION     DAOQMF010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54011_length_1680_cov_3.726154"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLV77_08195"
     CDS             <1..314
                     /locus_tag="PLV77_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNC15866.1"
                     /translation="RLIATGPYVTEGRDIGTVVAPDSPLKVFLTASPEARAERRAAET
                     GQPVAEVLAAQEERDRRDSEREHGALYAAEDAVTIDSSGMSADQVIAEIAALARDRGL
                     A"
     gene            314..1660
                     /gene="der"
                     /locus_tag="PLV77_08200"
     CDS             314..1660
                     /gene="der"
                     /locus_tag="PLV77_08200"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNC15867.1"
                     /translation="MEAPGSSSAPSGTVPTVAIVGFPNVGKSTLVNRLAGGREAVVHS
                     EAGVTRDRKRVRCEWNGREFDLLDTGGIDLDDASEMAADIQRQARLGMAEADVVVLVV
                     NGQAGLRTGDTDLARELRTSPVPVIVAVNKSDRPDDEFAAAEFHALGLGDPTAVSATH
                     GIGTGDLLDRITEALGDRPARAEESDAVRVAVIGRPNVGKSSLVNRILGDDRVIVSPV
                     AGTTRDTIDTEIEVDGRSVILVDTAGLRRKSKVAGSVDHYAQLRSERAIERADVAIIV
                     CDAFEGVRSEDLKVGEVAMRAGCATMIVMNKWDITETDIDDTRARVGRKLRLRPEVVT
                     CSAKSGRNVAGLISRAVALADRASQRIPTPQLNRFISEVQTTTPPPSRRGRRLKLLYG
                     AQTGVSPPHFSIQVNDRRLIVRDWAYHLENRLRDQWGLDGVPLVIDYIPRKGRGDD"
BASE COUNT          277 a          577 c          569 g          257 t
ORIGIN      
        1 aacggctgat cgcgaccggg ccgtacgtga ccgaaggccg cgacatcggc accgtggtcg
       61 cccccgattc cccgctgaag gtctttctca ccgcctcccc ggaagcgagg gccgagcgcc
      121 gggcggccga gaccggccag cctgtggccg aggtgctggc cgcccaggag gaacgcgacc
      181 ggcgtgactc ggaacgtgag cacggagccc tctatgccgc cgaagacgcg gtgacgatcg
      241 acagcagcgg catgtccgcc gaccaggtga tcgccgagat cgccgctctt gcccgcgacc
      301 ggggcctggc ctgatggaag cgccaggcag ttcctcggcc ccgtcgggga cggtgccgac
      361 cgtcgccatc gtcggtttcc ccaatgtcgg caagtcaacc ctcgtaaacc ggctggctgg
      421 cggccgcgag gcggtcgttc actctgaagc cggggtcacc cgggatcgca agcgggttcg
      481 ctgcgagtgg aacggccgcg aattcgacct gctcgacacg ggtggcattg acctcgatga
      541 cgcgtcggag atggcggcgg acattcagcg ccaggcccgg ctcggcatgg ccgaggccga
      601 tgtcgtcgtg ctggtcgtca acggccaggc cggtctgcgc accggtgaca cggaccttgc
      661 ccgcgagcta cgcacctcgc cggtaccggt gatcgtggcg gtcaacaagt cggatcggcc
      721 ggacgacgaa ttcgccgcgg cggaatttca cgcgctcggc cttggtgatc cgaccgcggt
      781 ctctgccacc cacgggatcg gtaccggtga tctgctcgac cggatcaccg aggctctcgg
      841 agaccgtccc gcccgggcgg aggaatctga cgcggtgagg gttgcggtga tcggccggcc
      901 caacgtcggc aagtcctcgc tggtcaaccg gatcctcggc gacgaccggg tgatcgtttc
      961 cccggttgcc ggcaccaccc gggacacgat cgacaccgag atcgaggttg acggccgcag
     1021 cgtgattctg gtcgacaccg cggggcttcg tcgcaagtcg aaggtcgccg gttcggtcga
     1081 tcactacgcc cagcttcgct ccgagcgggc catcgaaagg gccgacgtgg cgatcatcgt
     1141 ctgcgacgcc ttcgaaggcg tgcgctccga ggacctgaag gtgggggagg tggcgatgcg
     1201 ggccggctgc gccacgatga tcgtgatgaa caaatgggac atcaccgaga ccgacatcga
     1261 tgacacccgg gcccgggttg ggcgaaagct ccgcctgcgc cccgaggttg tgacctgttc
     1321 ggccaagtcg ggccgcaacg tcgccggcct gatctcccgt gctgtcgcgc tggccgaccg
     1381 ggcctcccag cggatcccga ccccgcaact caaccgcttc atttccgaag tccagaccac
     1441 gaccccgccg ccttcccgtc gcggccggcg tttgaagctc ctctacggag cccagaccgg
     1501 cgtgtcgccg ccccatttct cgatccaggt caacgaccgg cgattgatcg tgcgcgactg
     1561 ggcctaccac ctcgagaacc gccttcgcga ccagtggggc ctcgacggag tcccgctggt
     1621 gatcgactac atcccccgaa aaggccgggg ggacgactga agacacgaaa aaggggtcac
//
LOCUS       DAOQMF010000493         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54016_length_1680_cov_3.497231, whole genome shotgun sequence.
ACCESSION   DAOQMF010000493 DAOQMF010000000
VERSION     DAOQMF010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54016_length_1680_cov_3.497231"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLV77_08205"
     CDS             <1..370
                     /locus_tag="PLV77_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15868.1"
                     /translation="RALAASLPEVTGDLPVIGVIGVLADKDAPGMLAELAGSLEAAVF
                     TGLPGEALAAWGRPGARAWDPEELQGLAGRLGLEAETARTPAGALERAREMALEKGGA
                     VIITGSHFLLAPGEGAGFQG"
     gene            565..1335
                     /locus_tag="PLV77_08210"
     CDS             565..1335
                     /locus_tag="PLV77_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC15869.1"
                     /translation="MPIAIFGIGNTALNLAVTLAILMLVAIWIALIVFTFTDARRRIA
                     DPFLVGCATAASFFPFVGTLVYTILRPPEFLEDIRERETDITESEVRLRHLKHSSCHK
                     CGSPVESDFIRCPACRSRLKEPCPTCSKPVGLNWKVCPYCETTLIAPKRSSRSSGSKR
                     PKGESSGRPSSSRSGRSERAPEGKEERRRRSSKAPAESGARESRSSSGESRPEDSSRR
                     SGNSSSDQADSSRSPRSARRKISVSDDEKSEPINRGDS"
     gene            1427..>1680
                     /locus_tag="PLV77_08215"
     CDS             1427..>1680
                     /locus_tag="PLV77_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNC15870.1"
                     /translation="MSQTLILVKPDAFERALTGEVLARFERKGLRIAALKKLTATEQI
                     ANEHYAEHKEKPFFGELVEFITGGPLVAAVLEGPEAVVAAR"
BASE COUNT          272 a          590 c          529 g          289 t
ORIGIN      
        1 ccgtgcgctg gccgcctcgc tgcccgaggt gaccggggat ctgccggtga tcggcgtgat
       61 tggcgtgctt gccgacaagg atgccccggg gatgctggcc gaactggccg gctcgcttga
      121 agcggccgtc ttcaccggtc tgcccgggga agccctcgcg gcctggggca ggcccggggc
      181 acgggcctgg gatccggagg agcttcaggg ccttgccggg cggctcgggc tcgaggccga
      241 aacggccagg accccggccg gggcgctgga aagggccagg gagatggctt tagagaaggg
      301 cggagcggtg atcatcaccg gttcgcactt ccttctcgca ccgggcgaag gtgccggctt
      361 ccagggctga acgacgggca aggtctctca gaagccgggg cggagggttc ggcagccgcc
      421 ggttatgcga cgattagccg gtgaatcgtc agactggctc ccaactgctg tcgatgatgg
      481 cgctggtcgc tgcggtcgtg gcgatcgtca ttctcgtctt cttcggcatt gggtacctat
      541 tcggaaggat gttcctctag tcacatgcct atcgccatct tcggaattgg aaataccgcg
      601 ctgaacctgg ccgtcactct ggccatcctg atgctggtcg cgatctggat cgccctcatc
      661 gtcttcacct tcaccgacgc ccgtcgccgg atcgccgacc ccttcctggt cggctgcgcc
      721 accgccgctt cgttcttccc cttcgtcggc acgctcgtct acacgatcct tcgtccgccg
      781 gagttcctcg aggacatccg tgagcgcgaa accgacatca ccgagtccga ggtgcggctg
      841 cggcacctca agcacagctc ctgccacaag tgcggctccc cggtcgagag tgacttcatc
      901 cgctgcccgg cctgccggtc ccggctgaag gagccgtgcc cgacctgctc gaagccggtc
      961 ggcctgaact ggaaggtctg cccctactgc gagaccaccc tgatcgctcc caagcggtca
     1021 tcgcgcagct ccggctcgaa gcgccccaag ggcgaatctt cgggtcgtcc atcatcttcc
     1081 cgctctggcc gctccgagag ggctcccgaa gggaaggaag aacgccgtcg ccgctccagc
     1141 aaggccccgg ccgagtcggg tgcccgcgag agccggtctt cgtccggcga aagccgcccc
     1201 gaggattctt cccggcgttc gggcaactct tcttccgacc aggccgattc gtcccgttcg
     1261 cctcgttccg cccggcgcaa gatcagcgtc agcgacgacg agaagtcgga accgatcaac
     1321 cgcggcgaca gctgaacccc tgcgcgggta cgctgacccg ccggctcacg ccaaacgaaa
     1381 cccgctggct tcggccacaa ccaaactcac aacggagtaa caagacatgt cccagaccct
     1441 gatcctggtc aagcccgacg ccttcgagcg cgccctcacc ggtgaggttc tcgcccgctt
     1501 cgagcgcaag ggccttcgta tcgccgccct caagaagctg accgcgaccg agcagatcgc
     1561 caacgagcat tacgccgagc acaaggagaa gcccttcttc ggtgaactgg tcgagttcat
     1621 caccggcggc ccgctggtcg ccgccgtcct cgaaggcccg gaagcggtcg tcgccgcccg
//
LOCUS       DAOQMF010000494         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54035_length_1680_cov_2.500923, whole genome shotgun sequence.
ACCESSION   DAOQMF010000494 DAOQMF010000000
VERSION     DAOQMF010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54035_length_1680_cov_2.500923"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /gene="msrA"
                     /locus_tag="PLV77_08220"
     CDS             complement(<1..499)
                     /gene="msrA"
                     /locus_tag="PLV77_08220"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNC15871.1"
                     /translation="MFFGRDKTTMPAPEDSLPGRSGYAFPVPDNHTVLGTPIQGPFPE
                     GIEVAHFALGCFWGAERLFWNLDGVYTTAVGYQNGFTPFPTYEEVCSGKTGHAEAVLV
                     AFDPEKVSYERLLEVFFSEHDPTQGMRQGNDVGTQYRSAAFTVGDRQKEIAEMACDTY
                     QRALNE"
     gene            complement(541..>1680)
                     /locus_tag="PLV77_08225"
     CDS             complement(541..>1680)
                     /locus_tag="PLV77_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15872.1"
                     /translation="LSWELVQRPLKGDYLSRADVFRYSRRSGQVGADELELAGSFRGR
                     QVTTQRIQFRLGNVPASSVSVAAFGDSVTAGFGYFGATGKPMTLGQLLDCKPGATTLN
                     DACSSNSYNRNSSVGSKPNYLQDFGLSRNISWAAQWANEYGITEYENYAVTGSAPADW
                     LPNGQFHQTLQNIESQNPDYILMTLGANPLLDDVLFDVDTMGCALESDLFGDFRQCVL
                     DAFASVNLDSNMNQIFTDLVQNSSSHVVLMGYPFTIPSSALAYSAAQLEMMESLLNEV
                     IADEAESVSSSRITVVTPPRFNVGIDMEPLYPSNYSCSLLGYKVDGPSVQSTPTQDEL
                     EIDHPLSFCSGPSFGKPWVISGDTGIHPSAAGYLQMSSQIPAPGS"
BASE COUNT          300 a          496 c          574 g          310 t
ORIGIN      
        1 gctcgttgag tgcgcgctgg taggtgtcgc aggccatctc ggcgatttcc ttctgccggt
       61 cgccgaccgt gaaggcggcg gagcggtact gggttccgac gtcatttccc tgacgcatcc
      121 cctgggtcgg gtcgtgctcg gagaagaaga cctctagcag ccgctcgtag ctgaccttct
      181 ccggatcgaa ggcgaccagc accgcctcgg cgtggccggt cttcccggag cagacctctt
      241 cgtaggtcgg gaagggcgta aacccgttct ggtagccgac cgcggtcgtg tagacaccgt
      301 cgaggttcca gaaaagccgt tccgcgcccc agaagcagcc gagcgcgaaa tgggcgacct
      361 cgatcccttc cgggaagggt ccctggatcg gggttcccag aacggtgtga ttgtccggaa
      421 ccgggaaggc gtagccggag cggcccggga gtgaatcttc cggggcgggc atcgtggttt
      481 tgtcgcgacc gaagaacatg gaaccgatgc tactcgggtg cccttcccgg ttcgcgagct
      541 tcaggacccg ggcgcgggaa tctggctgga catctgcagg tagccggcgg cactcgggtg
      601 gatcccggtg tcgccgctga tcacccaggg cttcccgaac gagggtccgg aacagaagga
      661 gagcgggtgg tcgatctcca gttcatcctg ggtcggggtc gactgaacgc tcgggccatc
      721 gaccttgtag ccgagcagcg aacaggaata gttcgagggg tagagcggct ccatgtcgat
      781 tccgacgttg aagcggggcg gggtcaccac ggtgatccgg ctgctcgaga ccgattcggc
      841 ctcgtccgcg atcacctcgt tgagcagcga ttccatcatc tcgagctggg ccgcgctgta
      901 ggcgagtgcc gaggatggaa tcgtgaacgg gtaacccatc agcaccacat gcgaactcga
      961 gttctggacc aggtccgtga agatctggtt catgttcgag tcgaggttga ccgaggcgaa
     1021 ggcgtcgagg acgcattggc ggaagtcgcc gaagagatcc gactccagag cgcagcccat
     1081 ggtgtcaacg tcaaagagca cgtcatcgag cagcggattg gccccgagcg tcatcaggat
     1141 gtagtccggg ttctgcgact cgatgttctg gagggtctga tggaactgac cgttcggcag
     1201 ccagtcggcc ggcgcggaac cggtcaccgc gtaattctcg tattcggtga tgccgtattc
     1261 gtttgcccat tgcgcggccc aggaaatgtt ccgcgagagc ccgaagtcct gcagatagtt
     1321 cggcttgctc ccgaccgagc tgttgcggtt gtaggagttc gaggagcagg cgtcgttcag
     1381 ggtcgtagct cccggtttgc agtcgagcag ttgcccgagg gtcatcggct tgccggtcgc
     1441 cccgaaatac ccgaagccgg cggtcaccga gtcgccgaac gccgcgaccg agacactgga
     1501 tgccggcacg ttgccgagac ggaactgaat ccgctgggtc gtcacctggc gcccccggaa
     1561 agagccggcg agttccagtt cgtcggcacc gacctgaccg gacctccgcg agtagcggaa
     1621 cacgtccgcc ctgctcaggt agtcgccctt gagaggtcgc tggacgagtt cccaggagag
//
LOCUS       DAOQMF010000495         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54339_length_1675_cov_2.775309, whole genome shotgun sequence.
ACCESSION   DAOQMF010000495 DAOQMF010000000
VERSION     DAOQMF010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54339_length_1675_cov_2.775309"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLV77_08230"
     CDS             complement(<1..366)
                     /locus_tag="PLV77_08230"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC15873.1"
                     /translation="MSFTTSWKKIMLLLAATMALAVGVAACGSSDDDSGDSGSSTAGG
                     TQDATLVLDFVPGAVHSGIYEAVQKGYYEDNGINLKIIEPTSTADTLKLIDAGKAEFG
                     IADGIDLATQISDGREAQGI"
     gene            complement(437..1069)
                     /locus_tag="PLV77_08235"
     CDS             complement(437..1069)
                     /locus_tag="PLV77_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNC15874.1"
                     /translation="MAEAARIITGAGLVEAFGHVSARAGDGFLITATRPLGGAGERDV
                     YRVDADGEAPEGATDLPLETPMHAAVYAARPEVQAICRTHSPAAVRAGARQVVPPLVH
                     GLGGLAGEVALCGRTDLVTDREAGGEISRDLGSADCLLIRGNGSIATGRSLAEAVVRA
                     RYLEERCLAGAGMGQEEGWRADLVDPRARWFEKETTRAWAWMRWRYGGEK"
     gene            complement(1099..>1675)
                     /locus_tag="PLV77_08240"
     CDS             complement(1099..>1675)
                     /locus_tag="PLV77_08240"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HNC15875.1"
                     /translation="EVIVPAREARTMEVKKGQTLEIVDLEGQQVGDFTAWLASDPNEY
                     LSPSHTVSSLSKLVPEVGDSILSNHRRPLLKILRDDVGRHDLVVPCCDPERYAIDYGE
                     PDHGSCLLSLETAIEGTNLPLRGEMCWNVFMNNQLEDGKIVTYEPPHPAGSTIAMEVL
                     EDMTVALSACPQDISAVNAFNPTPMAMRVSD"
BASE COUNT          254 a          578 c          546 g          297 t
ORIGIN      
        1 gatgccctgg gcttcccggc cgtcgctgat ctgggtcgcc aggtcgatgc cgtcggcgat
       61 gccgaactcg gccttgccgg cgtcgatcag cttcagcgtg tcggcggtcg aggtcggctc
      121 gatgatcttg aggttgatgc cgttgtcctc gtagtagccc ttctggacgg cctcgtagat
      181 gccggagtga acggcgccgg gcacgaagtc cagcaccagg gttgcgtcct gggtgccacc
      241 ggccgtgctg gagccggagt cacccgagtc gtcgtcgctc gagccacagg cggcgacccc
      301 gacggcgagc gccatcgttg cggccagcag caacatgatc ttcttccaac tggttgtgaa
      361 actcatgagt cctctctctg gttgtagttc gaaacacccg ccccggttct cacccgggac
      421 cggttcaacg cgatccctat ttttctccgc cgtacctcca tcgcatccag gcccaggccc
      481 gggtcgtttc cttttcgaac catctggcac ggggatccac gaggtcggcc ctccaacctt
      541 cctcctggcc catacccgct ccggccagac acctttcctc cagatagcgg gcccggacca
      601 ccgcttcggc caggctgcgc ccggtcgcga tcgaaccgtt cccgcggatc agcaggcaat
      661 ccgcgctgcc aagatctctt gagatctcgc ccccggcctc ccgatcggtg accaggtcgg
      721 tgcggccgca gagcgcgact tcaccggcca gcccgccgag accatgcacc aggggcggca
      781 cgacctgccg ggcgccggcc cgcaccgcgg ccggcgaatg ggtccggcag atcgcctgga
      841 cctcgggccg ggccgcatag accgccgcgt gcatcggcgt ctcgagcggc aagtcggtcg
      901 ccccctcggg cgcttcgccg tcggcgtcga cccggtacac gtcccgctcg ccggctccgc
      961 cgagcgggcg ggtagcggtg atcaggaagc cgtcacccgc gcgcgccgaa acgtggccga
     1021 aggcttcgac caggcctgcc ccggtgatga tccgggccgc ttcggccacc tcggccgcgc
     1081 tcgtatgagt cgatccagtc agtcgctcac cctcatcgcc atcggggtcg ggttgaaggc
     1141 atttaccgcg gagatgtcct gggggcaggc cgaaagggcg acggtcatgt cttccagcac
     1201 ctccatcgcg atcgtcgatc cggccggatg gggcggctcg taagtgacga tcttgccgtc
     1261 ctcgagctgg ttgttcatga agacgttcca gcacatctcg ccgcggagcg ggagattcgt
     1321 gccctcgatc gcggtctcca gcgaaagcag gcaggagccg tggtcgggct cgccgtagtc
     1381 gatcgcgtag cgctcgggat cgcagcacgg aacgacgagg tcgtggcggc cgacgtcatc
     1441 gcgaaggatc ttcagcagcg gccggcgatg gttggagagg atcgagtccc cgacttccgg
     1501 caccagcttc gagagcgagg agaccgtgtg ggaaggcgac aggtactcgt tcgggtcgga
     1561 cgcgagccag gcggtgaagt caccgacctg ctggccttcg agatcgacga tctccagggt
     1621 ctgccctttc ttcacttcca tggtccgtgc ctcgcgggcc ggaacgatca cttca
//
LOCUS       DAOQMF010000496         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54401_length_1674_cov_2.890673, whole genome shotgun sequence.
ACCESSION   DAOQMF010000496 DAOQMF010000000
VERSION     DAOQMF010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54401_length_1674_cov_2.890673"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLV77_08245"
     CDS             complement(<1..183)
                     /locus_tag="PLV77_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC15876.1"
                     /translation="MEKRKLGSEGLEVSAIGLGCMGMSEFYGEGDEAESIATIHRALE
                     VGVNFLDTADMYGPWTN"
     gene            complement(343..909)
                     /locus_tag="PLV77_08250"
     CDS             complement(343..909)
                     /locus_tag="PLV77_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15877.1"
                     /translation="MNTSRASAVAAAFAAIAFIAFFSFAGHAQAAGCKDASSGGLTLA
                     PADCKPVKKARLIRGIAYAPDNAPAAVKRVIAAANRIRLKPYIYGGGHTLSRRLQAGY
                     DCSGSVSYALRAGGFISYPMPSGSFTNWKQPGAGKWITTYANGGHMYMIVAGLSFDTS
                     NMSSSGGNRWSTSIRSSRGFSVRHPGGF"
     regulatory      complement(947..1092)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1404..>1674
                     /locus_tag="PLV77_08255"
     CDS             1404..>1674
                     /locus_tag="PLV77_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC15878.1"
                     /translation="MDIAGKKVLLTGATGGIGRRIASTLAAAGAEVIVSGRSAEALTE
                     LVDSLPGSGHSVLAADLGLVGAAQELASQAGAVDMLVANAALPSTG"
BASE COUNT          274 a          513 c          572 g          315 t
ORIGIN      
        1 gttggtccag ggcccgtaca tgtcggcggt gtcgaggaaa ttgactccga cctccagggc
       61 ccggtggatg gtcgcgatcg attccgcttc gtcgccttca ccgtagaact cggacatgcc
      121 catgcagccc agcccgattg cggaaacctc gagcccttcg ctgcccagct tgcgtttctc
      181 catctgtcga tcatagaacc ggcgcgctcc ccggccccct cgtccccgga atcatggccg
      241 gtcgacccgg tcgtgccacg ccgggcggga agggaacggg ttgatgcaag agaaacgccc
      301 cggacttgcg gggcgtttcg cgattctgct gtgtcggcag gtctagaagc cgccggggtg
      361 cctgacgctg aatcccctgg atgaccggat cgaggtgctc cagcgattgc cgccggagga
      421 gctcatgttt gaagtatcga acgagaggcc agccacgatc atgtacatgt gaccgccatt
      481 ggcgtaggtg gtgatccact tgccggcacc gggctgcttc cagttggtga agctgccgga
      541 aggcatcggg tagctgatga agccgccggc gcggagcgcg tagctgaccg agccggagca
      601 gtcatagccg gcctgcagcc tgcggctgag ggtgtgaccg ccaccgtaga tgtacggctt
      661 gaggcgaatc cggttggcgg cggcgatgac ccgcttgacg gcggcgggag cgttgtccgg
      721 ggcgtaggcg atgccccgga tcagccgggc cttcttgacc ggcttgcagt cggccggggc
      781 gagggtgagc ccgccactcg aggcgtcctt gcagccggcg gcctgggcgt ggccggcaaa
      841 ggagaagaag gcgatgaagg caattgcggc aaatgcggcg gcaacggcgg aagcccggga
      901 agtattcaaa gcaactgacg tctgattttt gtgaaacagg ttgataccga actccttggt
      961 cggcctacgg ggttagctgt cgggctggcg ctttaggagc cgcgccccca cctgaatcag
     1021 tgggttcgcc ccagaaactt ggttcccccg atcccggtca cagagtgagt cgggattcgg
     1081 cgtctcggtt atgaacttgc gggatcatag acgatctgca tgtcattcgc taacaaaccc
     1141 cccgatccct ttgttcatcc ctgcagtttc catacataag tagtcggcga gcttcgcccc
     1201 gcctccatgc tcaacccgcc ggcccaggac acccgggaac ggatgggccg gctccgttcg
     1261 gtgaagcttc aggcctgatc accctggagg cacttccgct ggagtcccgc ccaaccattc
     1321 tttgcttcga gtgggcgggg cgggtttctc tgggagggcg ggttggaccc cgcccgactc
     1381 cttgtgtcgg ctaggtttga ctggtggata tcgcgggcaa gaaggtgctt ctgaccggtg
     1441 cgaccggagg tatcgggcgc aggatcgcga gcaccctggc ggccgccggg gcggaggtga
     1501 tcgtgtccgg ccgttcggcc gaggcgctca ccgagctggt cgacagcctg cctggcagcg
     1561 gccactcggt tctggcggcc gacctcggac tcgttggtgc cgcgcaggaa cttgccagcc
     1621 aggccggagc ggttgacatg ctggtcgcca atgcggctct gcccagcacc ggtc
//
LOCUS       DAOQMF010000497         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54577_length_1671_cov_4.062500, whole genome shotgun sequence.
ACCESSION   DAOQMF010000497 DAOQMF010000000
VERSION     DAOQMF010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54577_length_1671_cov_4.062500"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLV77_08260"
     CDS             complement(<1..335)
                     /locus_tag="PLV77_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15879.1"
                     /translation="MRSGIRSLRVDRERAIRLGAIIGLVLLGASTLPGLLKTPDPPPV
                     PANVGFRPAEMNGFAAQPGPGEAIRKEAAKKKRERTLKRKQLEAGRKRRARKREESRR
                     RRASKRVRE"
     gene            449..844
                     /gene="rpsF"
                     /locus_tag="PLV77_08265"
     CDS             449..844
                     /gene="rpsF"
                     /locus_tag="PLV77_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNC15880.1"
                     /translation="MTDSSRQYELVLMLDPQVEEAAREKLVSETKSAIEANGSLKHED
                     NWGIREMAYEIDRKSEADYRLFRFDGERELLGTLDHSLKIADGVLRFRIFRVDPDAPV
                     LPAPPATAVVMAPTGDDDERRGRGRYRDE"
     gene            1019..1537
                     /gene="ssb"
                     /locus_tag="PLV77_08270"
     CDS             1019..1537
                     /gene="ssb"
                     /locus_tag="PLV77_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC15881.1"
                     /translation="MAENINRVTITGNLTRDPELRQTPTGTAVCTLGVAVNGRRKEGD
                     QWVDKPNYFNVVVWGAQGQNCANYLSKGRPVAVDGRLDWRSWEAKDGSGKRSTIEIVA
                     DTVQFLGSRDGSAPGGNNGGHSEANNGGNYGGQAQGGQGGGFTPSSDVPADPGDFAPT
                     SGGAPADDDIPF"
BASE COUNT          291 a          566 c          528 g          286 t
ORIGIN      
        1 tgttctctga cccgcttcga tgcccgccgc ctccgggact cttctctttt ccgcgctcgc
       61 ctctttcgcc cggcttcgag ctgcttccgt ttcagcgtcc gctcccgctt cttcttcgca
      121 gcttccttgc ggatcgcctc cccgggaccg ggttgcgctg cgaacccgtt catctcggcc
      181 gggcggaatc ccacgttggc cggaaccggc ggagggtccg gggttttcag cagaccgggc
      241 agggtcgagg caccgagcag gacgaggccg atgatcgcgc ccagcctgat cgcgcgctcg
      301 cggtcaaccc tgaggctccg gatgccactt cgcatacctg atcaagggcc cgggcgggca
      361 gactctcccc ctggagggac cggaaccggg ttgtaatcca catcgcaccg cggaaatcgg
      421 gcgatgaagg tatagtcccc cgtcgatttt gactgactca agccgccaat acgaacttgt
      481 cctgatgctc gacccccagg ttgaggaagc agcccgtgag aagctggttt ccgaaaccaa
      541 gtcggccatt gaagccaacg gttcgctcaa gcacgaagac aactggggca tccgcgagat
      601 ggcctatgaa atcgaccgca agagtgaagc cgactaccgg ctgttccgct tcgacgggga
      661 gcgcgagctg ctcggcacgc tcgatcacag cctgaagatc gccgacggcg tcctgcgctt
      721 ccgcatcttc cgggtcgacc cggacgctcc ggtcctgccg gccccgcccg ccaccgccgt
      781 ggtgatggcc ccgaccggtg acgatgacga gcgccgcggt cgcggtcgct accgcgacga
      841 gtaaggcatc caggccgtgg tgccgtgaat ctttccgcgg tatttgcgta atcggggggc
      901 gagaattcgc cccctttgcc tttaagcgtc cacaggcgtc gctacattcc tcctccgacc
      961 cgcgggttcg acaggggatc cgcgatgtac gaaaaacgcg ataaggagca aacacacaat
     1021 ggccgagaac atcaacaggg tcacgatcac cggcaacctc accagggatc cggaactgcg
     1081 gcagacgccg accggtactg ccgtatgcac tctcggagtg gccgtaaatg gccgccgcaa
     1141 ggagggcgat cagtgggttg acaagcccaa ctacttcaac gtggtggtct ggggcgcaca
     1201 ggggcagaac tgcgccaact atctttcgaa gggtcgtccc gtcgcggttg acggccggct
     1261 cgactggcgc tcctgggagg ccaaggacgg ttccgggaaa cggtcgacga tcgaaatcgt
     1321 cgccgacacg gtccagttcc tcggttcccg ggacggctcg gcccccggcg gcaacaacgg
     1381 cgggcacagc gaggccaaca atggcggcaa ctacggcggc caggcccagg gtggccaggg
     1441 cggaggtttc accccctcct cggacgttcc cgccgacccc ggagacttcg cccccaccag
     1501 cggcggagcc ccggccgacg acgacatccc cttctaaccg accgcctgac cgggtgcggg
     1561 ccttctcggg gtgccgcagg tttgcaaccg cagaggttgc aaacctgcgt caccctcgtc
     1621 ccttgactcc cgttggtgta gccgtggggt cgggtggggg tcgtccggga c
//
LOCUS       DAOQMF010000498         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54600_length_1671_cov_3.101485, whole genome shotgun sequence.
ACCESSION   DAOQMF010000498 DAOQMF010000000
VERSION     DAOQMF010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54600_length_1671_cov_3.101485"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLV77_08275"
     CDS             <1..542
                     /locus_tag="PLV77_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15882.1"
                     /translation="VQFLSDHAGKAEVFVDLGSGDGFFCAGVARKASPEVIGFETNQF
                     ECKLAHELARKNGVQIETRGLATEDGLNDLPRGRLLLLSDVEGLEEDLIDPEIVPRLR
                     EATMVIETHEQFRPNVIEKLNERFSDTHDISRIFKEVPGRKSCPEVKDWSDGDFEIAA
                     YDGHHSGDSWMTLVPKKPG"
     gene            612..1082
                     /locus_tag="PLV77_08280"
     CDS             612..1082
                     /locus_tag="PLV77_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNC15883.1"
                     /translation="MNRREQIEMTQEEAEAFLLERKVAVVTSFGPRGWPHSMPLWYVP
                     NGDRIRSWTFAKSQKVKNLERDPRATVLVEDGYDNYSELRGVMMETETLIHRDPNLVT
                     AYAESSVARYTPEGVELTTEMLEAFRSQAPKRVVLEFIPVRVLTWDHRKLGGTY"
     gene            complement(1128..>1671)
                     /locus_tag="PLV77_08285"
     CDS             complement(1128..>1671)
                     /locus_tag="PLV77_08285"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC15884.1"
                     /translation="RTLTAWDHLTGEPATRLEVASDPLGPTGGAALFDRVAFETIGGF
                     DERIFLYYEDLDLALRMRMAGYSCRLARAARATHESSATLGRRAAAKYRRTGFSRGYL
                     LRKYGIMAHPGLAIMTLISDGSASLAQLLHDRTAAGLNGRIEGWQAGRDIVPIDPPFE
                     LLEPPSLRRHMRVRLNRRRY"
BASE COUNT          363 a          453 c          529 g          326 t
ORIGIN      
        1 ttgtgcagtt tttgagcgat catgccggaa aggcggaagt gttcgtcgat ctcggatccg
       61 gggacgggtt tttctgcgca ggcgtggctc gaaaggcttc gccagaggtg atcggctttg
      121 aaacgaatca gttcgaatgc aagttggcac acgagttggc cagaaagaac ggggttcaga
      181 tcgaaacccg cggattggca actgaagacg ggctgaacga tctccccaga ggaaggctgc
      241 tcctgctttc cgacgtagag ggacttgagg aggatctcat cgaccccgag attgttcccc
      301 ggctcagaga agcgaccatg gtgatcgaaa cccacgaaca gttccgcccg aacgtaatcg
      361 aaaagttgaa cgaacggttc tctgacaccc atgacatctc caggatcttc aaagaagtac
      421 ccgggcgaaa gtcctgcccg gaagtgaagg actggagtga cggagatttc gagattgcag
      481 catacgacgg gcatcattcc ggtgacagtt ggatgactct cgtgccgaag aagcctggat
      541 gatcccgcag gatgactgcc cgcccacgct tcggtcgcat gatcggactt gcatcgagtt
      601 agggttaggg catgaaccgg cgtgaacaga tcgagatgac ccaagaggaa gccgaggctt
      661 ttctacttga gcgcaaagtg gctgtcgtga ccagtttcgg ccctcggggc tggcctcatt
      721 cgatgccgct ctggtacgtc ccgaacgggg accggatcag gtcctggact ttcgccaagt
      781 cccagaaggt caagaacctc gaacgtgatc cgcgcgcgac cgtgctagtc gaggatggct
      841 acgacaatta ttccgagctc aggggcgtga tgatggagac cgagacgctc atacatcgtg
      901 atcccaacct ggttaccgca tacgcggaga gttcggttgc ccgatatacg cctgaagggg
      961 tcgagctcac caccgaaatg ctcgaggcgt ttcgtagtca agcgccaaag agggtggtcc
     1021 ttgagttcat tccagtcaga gttctgacct gggatcatcg gaaactggga ggaacctact
     1081 gaccggtgga tcggttgcga gattgggcaa gtgagccaca gtggagctca gtaacgtcgg
     1141 cgattgagcc gcacccgcat gtgacgacgc aagctgggcg gttcgagcag ctcaaagggg
     1201 gggtcgattg gaacgatgtc gcgccccgct tgccagcctt caattcggcc gttaaggcca
     1261 gctgcagtcc ggtcgtgaag cagttgggcg aggctggccg agccatcgct gatgagggtc
     1321 atgatcgcca gtcctggatg agccatgatc ccgtatttcc tcaacaggta gccgcggctg
     1381 aagccggtcc gtcggtactt ggcggcagcc ctccggccta gggtcgcgga actctcgtgg
     1441 gtcgccctcg ccgctcttgc cagacggcag gaatagcctg ccatacgcat tcgcagggca
     1501 aggtcgagat cctcgtagta gaggaagatt cgctcatcaa accccccaat cgtttcgaaa
     1561 gcgacccggt cgaagagggc ggcgccgccg gtcgggccca aggggtccga cgcgacttcc
     1621 aagcgcgttg ccggttctcc agtcagatga tcccaggcag tgagggtcct g
//
LOCUS       DAOQMF010000499         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54770_length_1668_cov_3.241785, whole genome shotgun sequence.
ACCESSION   DAOQMF010000499 DAOQMF010000000
VERSION     DAOQMF010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54770_length_1668_cov_3.241785"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLV77_08290"
     CDS             <1..475
                     /locus_tag="PLV77_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC15885.1"
                     /translation="VAAALTFFVLAQSSTVRNQATDLAVGGIGVDTIERATLSLTVIA
                     VIVGALQVGVLMTRVVLQRMQEIGVLKAAGVSSRDIFLVFLFESLIYGFTGGVIGCLV
                     GLLIALLGPMPGSEEILRAFVITVALSTVVATLAGLAPARRAVQAPAVEAINHAW"
     gene            505..1257
                     /locus_tag="PLV77_08295"
     CDS             505..1257
                     /locus_tag="PLV77_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC15886.1"
                     /translation="MNEHEQVPGGTGPVVETEDLWKLFKLGDEVVKALKGVSLKIHPG
                     ELVVIMGPSGSGKSTMLHLMGGLERPTKGEIYLEGQPLSGLTESQLSTMRHQAVGFIF
                     QSYNLIPLLTATENVELPLVFDDVDSGQVRRDCVELLERVGLGHRLDHTPSRMSGGEQ
                     QRVAIARALIGKPRLLLADEPTANLDHKNGDAIVKLLTTLNHEMGMTTVISTHDRSVA
                     EHASRVIEMRDGKVVSDSGSGDGAPADRLATA"
     gene            1265..>1668
                     /locus_tag="PLV77_08300"
     CDS             1265..>1668
                     /locus_tag="PLV77_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC15887.1"
                     /translation="MTAISSVNPFDPSDQVAGCEGASAAEIDSVLAAGVAAQPGWAAD
                     PMLRLRSLSTLADEIEVRRQKFIDLMVREVGKPVAEAAGEVDRAIAILGFYSQVPLDP
                     VGEVVPGSVPGARVLVERHPLGLVLAICPWNFP"
BASE COUNT          264 a          563 c          556 g          285 t
ORIGIN      
        1 cgtcgcggcc gccctcacct tcttcgtcct cgcccagagt tcgaccgttc gcaaccaggc
       61 caccgatctc gcggttggcg ggatcggggt ggacacgatc gagagggcga ccctttcgct
      121 gaccgtgatc gccgtgatcg tcggcgccct ccaggtcggc gtgctgatga cccgggtggt
      181 gctgcagcgg atgcaggaaa tcggcgtgct caaggcggcc ggagtatcga gccgggacat
      241 cttcctcgtc tttctcttcg agtccctgat ctacggcttc accggcgggg tgatcggctg
      301 cctggtcggt ctcctgatcg ccctgctcgg accgatgccg ggcagcgagg agattctgcg
      361 ggccttcgtg atcacggtcg ccctttccac cgtcgttgcc accctggccg ggctggcgcc
      421 cgcccgtcgc gccgttcagg ccccggccgt agaagcaatc aaccatgcct ggtgagatga
      481 atgccgccgg ctgaaggaga tccgatgaac gaacacgagc aagtcccggg cggcaccggg
      541 ccggtggtcg agaccgaaga cctgtggaag ctgttcaagc tcggcgatga ggtggtcaag
      601 gcgctcaagg gcgtcagcct gaagatccac cccggtgaac tcgtcgtgat catgggcccc
      661 agcggcagcg gcaagtcaac gatgctgcac ctgatgggcg ggctggagcg gccgaccaag
      721 ggcgagatct acctcgaagg ccagccgctc tccgggctga ccgagagcca gctctcgacc
      781 atgcgccatc aggcggtcgg tttcatcttc cagtcctaca acctgatccc gctgctgacc
      841 gcgaccgaga acgtcgaact gcccctggtc ttcgacgacg ttgacagcgg ccaggtccgg
      901 cgtgactgcg tcgagctgct cgagcgggtc ggcctcggtc accggcttga tcacactccg
      961 agccgcatgt ccggcggcga gcagcagcgg gtggcaatcg cccgggccct gatcggcaag
     1021 ccgcgcctgc tcttggccga cgagccgacc gcaaaccttg accacaaaaa cggcgacgcg
     1081 atcgtcaagc tgctgaccac cctcaaccac gagatgggca tgaccaccgt gatctcgacc
     1141 cacgaccgct cggtggccga gcatgccagc cgggtgatcg agatgcggga cgggaaggta
     1201 gtttccgact ccggttcggg ggacggcgcc ccggccgacc gtctcgccac cgcctgagga
     1261 agccgtgaca gcgatcagca gcgtcaatcc ctttgatccc tccgaccagg ttgccggttg
     1321 cgagggtgct tccgcggccg aaatcgattc ggtcctcgcc gccggggtgg cggcccagcc
     1381 gggctgggcg gccgacccga tgctcaggct gcgttccctt tcgaccctcg ccgacgagat
     1441 cgaagtccgg cgccagaagt tcatcgacct gatggtgcgg gaagtcggca aaccggtcgc
     1501 cgaggcggcc ggcgaagttg accgggcgat cgcgatcctc ggcttctatt cccaggttcc
     1561 gctcgatccg gtcggggagg tggtcccggg ctcggtgccg ggcgcccggg ttctggtcga
     1621 gcgccatcca ctcgggctgg tgctggcgat ctgcccctgg aacttccc
//
LOCUS       DAOQMF010000500         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_54826_length_1667_cov_4.057072, whole genome shotgun sequence.
ACCESSION   DAOQMF010000500 DAOQMF010000000
VERSION     DAOQMF010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54826_length_1667_cov_4.057072"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLV77_08305"
     CDS             <1..341
                     /locus_tag="PLV77_08305"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNC15888.1"
                     /translation="QDLSLSFNHFFSRKVMFVKHAVAYVVMPGGFGTLDEMFEALTLI
                     QTLKARPHPVVLAGADFWQDLIDWIARRLAGEGRISPSDMDLFTVCDEDDEIISSAIH
                     GLPNLPYGLA"
     gene            395..730
                     /locus_tag="PLV77_08310"
     CDS             395..730
                     /locus_tag="PLV77_08310"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC15889.1"
                     /translation="MALPTPLPPELVELITARFKLLSEPMRIRILDQLREGEQSVGGL
                     AEILDTSQQNVSKHLQALYAGGAVARRKEGNTVYYGIADESLMSMCEAVCEGVEQRIA
                     ELSQIVTQA"
     gene            811..1080
                     /locus_tag="PLV77_08315"
     CDS             811..1080
                     /locus_tag="PLV77_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNC15890.1"
                     /translation="MYFRSETMMEASCETKGRRGWYLERVLFAMAGTVSLAGGLLALL
                     VSPWFALLPVLAGINQWLYVTVGACPASLVLSRVTSLRSAAETGR"
     gene            1077..>1667
                     /locus_tag="PLV77_08320"
     CDS             1077..>1667
                     /locus_tag="PLV77_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC15891.1"
                     /translation="MNKYGPLGRLGRWTTGNFRVVAAVWLVIAIGLGFFAPKAEHALS
                     GAGWEATGSESVDARKIVDQEFGGMSSSALMVVVHSDDSAFGTAAFDRTLNQAASVLR
                     ESDDVKAVVMPSRKSWVSADGHTAVIQAGAAADSNRMVRAADDIKVDLAALDQAGTTV
                     SLTGSSGMWSDFNQANKEAMMKSELISWPVTLLILVL"
BASE COUNT          288 a          545 c          525 g          309 t
ORIGIN      
        1 atcaagacct cagcctgagc ttcaatcact ttttttcccg caaggtgatg ttcgtcaagc
       61 acgcggtggc ctatgtggtg atgcccggcg gctttggcac gctggacgag atgttcgagg
      121 cgctgaccct gatccagaca ttgaaggcgc ggccgcatcc cgtcgttctc gccggggccg
      181 acttctggca ggacctgatc gactggattg ccaggcgcct ggcgggcgag ggccggatct
      241 ccccttccga catggacctc ttcacggtct gcgacgaaga tgacgagatc atcagcagcg
      301 cgattcacgg ccttccgaac ctgccttacg gactcgcctg aacgccgccg ggcaactcgg
      361 cgagcaaaca gttgtccggc tgtaagctga aggcatggcc ctgccgactc ccctcccgcc
      421 cgagctggtt gaactgatca ccgcccgctt caagctgctc tcggaaccga tgcggatccg
      481 gatcctcgat cagcttcgcg aaggagagca gagcgtcggt ggcctggccg agatcctcga
      541 caccagccag cagaatgtct ccaagcacct ccaggcgctc tacgccggag gcgcggtggc
      601 gcgccgcaag gaaggcaaca ccgtctatta cgggattgcc gacgaaagcc tgatgtcgat
      661 gtgcgaagcc gtctgcgagg gcgtcgaaca gcgcatcgcc gagctcagcc agatcgtcac
      721 gcaggcctga tcttccggaa cctcccgttc acgccgctca agccggcgcc ccgaatctct
      781 gctaatctat tccactatac agttgtgaac ttgtatttta gatcggagac gatgatggaa
      841 gcgagctgtg aaaccaaggg ccgccgtggc tggtacctcg aacgggtgct cttcgcgatg
      901 gccggaacgg taagcctggc cgggggcctc ctggccctgc tggtctcccc ctggttcgcc
      961 ctcctgccgg tcctggccgg gatcaaccag tggctttacg tcacggtcgg cgcctgtccg
     1021 gcgtcactgg tcctcagccg cgtcacttcg ctgcggtccg ccgcggaaac cggccgatga
     1081 acaagtacgg accactcggg aggctcggcc ggtggaccac cgggaacttc cgtgtagtcg
     1141 cggcggtctg gctggtcatc gcgatcgggc tgggcttctt cgcaccgaag gccgagcacg
     1201 cgctttccgg agccggctgg gaagcgaccg gctcggaatc ggtcgatgcc cgcaagatcg
     1261 tcgatcagga gttcggcggc atgtcctcct cggccctgat ggtcgtcgtt cacagcgacg
     1321 attcggcctt cggaaccgcc gccttcgacc ggaccctgaa tcaggccgcc tccgtgctca
     1381 gggagtcgga tgacgtcaag gccgtggtca tgccctcgag gaagagctgg gtctcggccg
     1441 acggccacac cgcggtgatc caggccggcg cggccgccga ctcgaaccgg atggttcgcg
     1501 ccgccgatga catcaaggtt gacctggcag ccctcgacca ggcggggacc acggtttccc
     1561 tgaccggctc ttccgggatg tggtccgact tcaaccaggc caacaaggaa gcgatgatga
     1621 agtcggagtt gatctcctgg ccggtgacgc tgctgatcct ggtcctc
//
LOCUS       DAOQMF010000501         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55088_length_1663_cov_4.325249, whole genome shotgun sequence.
ACCESSION   DAOQMF010000501 DAOQMF010000000
VERSION     DAOQMF010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55088_length_1663_cov_4.325249"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PLV77_08325"
     CDS             complement(<1..729)
                     /locus_tag="PLV77_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC15892.1"
                     /translation="MDTSRNIAARAGRWSATHRKTAIWGWLAFVILAVFIGGSVGTRT
                     LTDAEDGVGESGRAEVATSNNFPENATERVLITAPAGGSNTSAAMREAAADLSRELRA
                     TPYVSHVESPFAPGNRGQISADGKSALVSFELAGNEDQLSERLGATQEAVNEARARAP
                     GYGFAQFGDASASEQIGQAFEDDFKKAEFTSLPVTLLILVFAFGAFIAAGVPLLLGLT
                     SVAAAIGLTALVSHVTPMSDAANSV"
     gene            893..>1663
                     /locus_tag="PLV77_08330"
     CDS             893..>1663
                     /locus_tag="PLV77_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15893.1"
                     /translation="MGSALNLRARLTWLAIAGFLAGAGGATMIALTNQTLGEPVIEIT
                     LRVLTGWTWMGVGIFAWWRRPDNPFGKWMVFTSFAWLAQALTYANSSLVFTVGLVIAY
                     VFPFALIQTLVRFPTGRIETRADGWIVRLVAGALLLVLLATFFSSPDQLGYDNAPSNL
                     LLLADSPTTSAVFFIIANAVAIILVLGLIHSFTTKYSAASPPMRRLIGPVLWTSTTLL
                     AVLGLSSVFSIANLDTVEEIAGILGAIIMRGIMIGGGLY"
BASE COUNT          256 a          578 c          516 g          313 t
ORIGIN      
        1 caccgagttc gcggcgtccg acatcggagt gacgtggctg accagtgcgg tcaggccgat
       61 cgccgccgcg accgaagtca ggcccagcag caaaggtacg cccgcggcga tgaacgctcc
      121 gaaggcgaag accagaatca gcagggtgac cggaagcgag gtgaactcgg cctttttgaa
      181 gtcatcctcg aaggcctgtc cgatctgctc gctggccgag gcatcaccga actgggcgaa
      241 gccgtagccg ggcgcccggg cccgggcctc gttgaccgcc tcctgggtcg ccccgagccg
      301 ctccgagagc tggtcctcgt tgccggccag ctcgaaagag accagcgccg attttccgtc
      361 ggccgatatc tggccccggt tgccaggtgc aaaggggctc tcgacatgtg acacgtacgg
      421 cgtcgccctt agctcgcggg agaggtcggc ggcagcttcg cgcatcgcgg cgctggtgtt
      481 cgaaccaccg gccggcgccg tgatcagcac cctttcggtc gcgttctcgg ggaagttgtt
      541 cgaggtcgcc acttcggcgc gccccgactc gccaactccg tcttcggcgt cggtcagggt
      601 cctcgttcca accgatcctc cgatgaagac ggccaggatg acgaaggcga gccaacccca
      661 gatcgcggtc ttgcgatggg tcgcgctcca ccttccggca cgggctgcga tgtttcgact
      721 ggtatccatg ccgccaagcc tgcctttgcg gacgccggcg caaaatgggg cctgcctgtg
      781 tcttcagcgg gtgatgcctg ccccttaggg gttcggcaaa ccgcaccatg aacgcccgga
      841 tgcgggtact ggccgcacca acggaggtct ccgcatgggt cagaatcacc ccatgggtag
      901 tgctctgaac ctgcgggcga ggctgacctg gctggcgatc gccggcttcc tggccggagc
      961 cggcggggcg acgatgatcg ccctgacaaa ccagaccctg ggcgagccgg taatcgagat
     1021 cacgctgcgg gtgctgaccg gatggacctg gatgggagtc ggcatcttcg cctggtggcg
     1081 ccgccccgac aatccgttcg gcaagtggat ggtcttcacc agtttcgcct ggctggccca
     1141 ggccctgacc tacgcgaact cgtcactggt cttcaccgtc ggcctcgtca tcgcctatgt
     1201 ctttcccttc gccctgatcc agaccctggt ccgcttcccg accggccgga tcgaaacccg
     1261 ggccgacggg tggatcgtcc gcctggttgc cggcgccctc ctgcttgttc tgctggcgac
     1321 cttcttcagc tctcccgatc agctcggata cgacaacgcc ccgagcaacc tgctgctgct
     1381 ggccgactcg ccgaccactt cggccgtctt cttcatcatc gccaacgcgg tggcgatcat
     1441 cctcgtcctc ggtctgatcc acagcttcac gaccaagtat tcggccgcct cgcctccgat
     1501 gcgccgcctg atcggaccgg tcctctggac ctcgaccacc ctgctggccg tgctcgggct
     1561 ttcctccgtc ttctcgatcg ccaatctgga tacggtcgaa gagatcgccg gcatcctcgg
     1621 tgcgatcatc atgcgcggca tcatgatcgg cggaggcctc tac
//
LOCUS       DAOQMF010000502         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55198_length_1661_cov_4.135118, whole genome shotgun sequence.
ACCESSION   DAOQMF010000502 DAOQMF010000000
VERSION     DAOQMF010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55198_length_1661_cov_4.135118"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLV77_08335"
     CDS             complement(<1..624)
                     /locus_tag="PLV77_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HNC15894.1"
                     /translation="MALLILFGFIAGAATAVSPCVLPVLPVALSAGATGGRRRPAGIV
                     AGLTASFTFATVALVYVIDALGLPDDLIRNIAIAVLIGFGIVLLLPSVSARFEGWVSQ
                     FTARFTPQNSGDGFWSGTVVGASLGLVYAPCAGPILAGVITVSASQSFTVERFAIAFA
                     YALGSALVLYLLMLGGRRLITPLTKRVGWFQAAMGIVMIVFGIGMAAQ"
     gene            714..1661
                     /locus_tag="PLV77_08340"
     CDS             714..1661
                     /locus_tag="PLV77_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15895.1"
                     /translation="MADVRNYAKLTRRYAKFSLAGVLALLALVVLVIGGKPESAQAAP
                     VACPKFRVMNNDRIGKVSFPAGYYSVSLLNGNKLTCAQSTKLFQEFLQDWDGKLRSPW
                     RVYMSGNKRVFRAGNTDKGFTATPSGSSGGGGGGNSGRSCPSYFTVLHNDSIGSFKVP
                     RGKYRFTLIDPNKITCAQAAKRFQEFLLDFDGKLPYPWKLNPILGIFYNSLHGRIGFK
                     VNKAYGPSPSPKPDPSYGRCPATFRVLHNDRIGALSLPRGPYYIYVVNGLSCSSASNY
                     FRQFLNRTDGRLPAPWRLNVAKAKFNTGHGGIAFRVQHA"
BASE COUNT          335 a          575 c          504 g          247 t
ORIGIN      
        1 ctgggcggcc atgccgatcc cgaagacgat catcacgatc cccatcgccg cctggaacca
       61 gccgacccgc tttgtcaacg gcgtgatcag ccgacgacca ccgagcatca ggagataaag
      121 cacgagggcc gacccgagcg cataagcgaa ggcgatcgcg aatcgctcga cggtgaacga
      181 ttgcgaggcc gagacggtaa tcacgccggc gaggatcggg ccggcgcaag gggcgtagac
      241 gagaccgagg ctggcgccaa ccaccgtgcc cgaccagaag ccgtcacccg agttctgcgg
      301 tgtgaagcgc gcagtgaact ggctgaccca gccttcgaag cgggccgaga cggagggcag
      361 cagaagcacg atcccgaaac cgatcaggac cgctatcgcg atgttgcgga tcaggtcatc
      421 gggcaggccg agggcatcga tcacgtagac cagggcgacc gtggcgaagg tgaaggaggc
      481 ggtgagcccg gccacgatcc cggccggccg gcgcctgccg cccgtcgccc cggccgagag
      541 ggctaccggc agcactggca gcacgcaggg cgagacagcg gtcgccgccc cggcgatgaa
      601 cccgaacaaa atcagcaggg ccatgcctga agattcgcaa acggagcggc tttggatcac
      661 aaatagttcc gggatggccg gaacttgact gcggaagtag ggttcttctt catatggccg
      721 atgtccggaa ttacgcgaaa ctgacccgtc gatacgccaa gttctccctc gccggagtgc
      781 ttgccctgct ggccctcgtg gtcctcgtga tcggaggcaa gcccgagagc gcccaggcag
      841 ctccggttgc ctgccccaag ttccgggtca tgaacaacga ccggatcggc aaggtttcat
      901 tcccggccgg ttactactcg gtcagcctgc tgaacggcaa caagctgacc tgcgcccagt
      961 cgaccaaact tttccaggag ttcctgcagg actgggatgg caagctgcgc agcccctggc
     1021 gtgtctacat gagcggtaac aagcgggtct tccgggccgg caacaccgac aagggcttca
     1081 ccgcgactcc ctccggctcc agcggcggtg gaggcggcgg caactcgggc cgctcctgcc
     1141 ccagctactt cacggtgctg cacaatgact cgatcggcag tttcaaggtg ccacgaggca
     1201 agtaccgctt cacgctgatc gacccgaaca agatcacctg cgcccaggcg gccaagcgct
     1261 tccaggagtt cctgctcgac ttcgacggca agctccccta cccgtggaag ctcaacccga
     1321 tcctcggcat cttctacaac agcctccacg gccggatcgg cttcaaggtg aacaaggcct
     1381 acggcccctc gccgagcccg aagcccgacc cctcctacgg tcgctgcccc gccaccttcc
     1441 gggtccttca caacgaccgg atcggcgcac tctcactgcc gcgcggcccg tactacatct
     1501 acgtggtcaa cggactgagc tgctccagcg cctcgaacta cttccgccag ttcctcaacc
     1561 ggaccgacgg ccgcctgccc gccccgtggc gtctcaacgt ggccaaggcc aagttcaaca
     1621 ccggccacgg cggcatcgcc ttcagagtgc agcacgccta g
//
LOCUS       DAOQMF010000503         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55209_length_1661_cov_3.283313, whole genome shotgun sequence.
ACCESSION   DAOQMF010000503 DAOQMF010000000
VERSION     DAOQMF010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55209_length_1661_cov_3.283313"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..315
                     /locus_tag="PLV77_08345"
     CDS             133..315
                     /locus_tag="PLV77_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15896.1"
                     /translation="MKTLAFALVLLAGPVLAAELVTRTPVGIEALGLHVCLAALIVAP
                     LLLSAGFAWTRPQVRI"
     gene            315..704
                     /locus_tag="PLV77_08350"
     CDS             315..704
                     /locus_tag="PLV77_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNC15897.1"
                     /translation="MTNAENPPALSDLEREVMEQVWSASEEVSVRDVLNGINEANEKQ
                     RAYTTLMTILSRLEAKGMVTKRRKSKSDLFTPVLSRSDYLESRAKVEVDALVGEYGEI
                     ARALFVREIEDTDPDAAKKLKKLIDKS"
     gene            741..1379
                     /locus_tag="PLV77_08355"
     CDS             741..1379
                     /locus_tag="PLV77_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC15898.1"
                     /translation="MSDNVKLSIALVLVGALGLLVYLSLGNDTPEGSANPEVTRADSH
                     YLTKAPDDKVTIVEFLDFECEACKAQFPTMERIREEYDGRINLAIRYFPLGGHQNGMN
                     AALAVEAASKQGALEDMYIKMYETQAEWGESPNSKADLFAEYAQELGLDMDQFEADVK
                     DPETRKRVEKDQADGVGLGVQGTPTIYLNGEELPTMPSYEDLKQRIDALLAE"
     gene            1382..>1661
                     /locus_tag="PLV77_08360"
     CDS             1382..>1661
                     /locus_tag="PLV77_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNC15899.1"
                     /translation="MKVSSAGTATQAPAEFIQRVTPWVVTITSIVAFAAAAVLMVEKI
                     ILLGDPTYVPTCSINPVLSCGSIMKSDQAEAFGFPNPLLGLAGFGLMTT"
BASE COUNT          363 a          506 c          490 g          302 t
ORIGIN      
        1 gccggagcag gtcacggatg tgtatgcggt ggagcgggag tattggcgat ccctgtggcc
       61 ccgcatcgag gaagggggcc ggccttgagc cggcgcacct gtcgcagggg tcgtggggag
      121 cattctgaga ctatgaaaac gttggcattc gcgctggtct tgctggccgg cccggtactg
      181 gcagccgaac tcgttactcg cacaccggta ggcatcgagg ctttgggtct tcacgtttgc
      241 ctcgccgcgc tcatcgtcgc tcctcttctg ctttcggcgg gattcgcatg gacccgtccc
      301 caggtgcgga tctgatgacc aacgctgaga acccacctgc cctctcggac ctcgaacgtg
      361 aagtcatgga acaggtctgg tcggcatcgg aagaagtgtc ggtacgagac gtcctgaacg
      421 ggatcaacga agcgaacgag aagcagcgcg cctacacaac cctgatgacc atcctttccc
      481 gccttgaagc aaagggaatg gtcaccaagc gcaggaagtc gaagtccgac ctcttcaccc
      541 cggtgctctc ccgaagcgac tacctcgagt cccgagcaaa ggtcgaagtc gacgcactgg
      601 tcggcgagta cggcgagatc gcccgtgcct tgttcgtccg tgagatcgaa gacaccgatc
      661 ccgacgcagc caagaaactc aaaaagctga tcgacaagtc ctaattacta cagcttgtag
      721 tagcatcggc tgccaagtcg atgtcggaca acgtcaaact ctcaatcgcc ctcgtgctcg
      781 tgggcgcact cggacttctg gtctacctca gtctcggaaa cgacactccg gaaggatccg
      841 caaatcccga ggtcactcgc gcggactctc actacctgac caaggcgccg gacgacaagg
      901 tcacgatcgt agagttcctc gacttcgaat gcgaagcctg caaggcccag ttcccgacga
      961 tggagcgaat ccgcgaggaa tacgacggca ggatcaacct agctatccgg tacttcccac
     1021 tcggcggtca tcagaacgga atgaacgccg ccctcgcggt ggaggcggcc tccaagcagg
     1081 gagccctcga ggacatgtac atcaagatgt acgaaaccca ggccgagtgg ggtgagtcac
     1141 caaactcgaa ggcggacctc ttcgccgagt acgcccagga actcggactc gacatggacc
     1201 aattcgaggc cgatgtgaaa gatcctgaaa cgagaaagcg ggtcgagaaa gaccaggctg
     1261 acggtgtagg cctcggggtc cagggaacgc cgacgatcta tctgaacggc gaagagttgc
     1321 cgaccatgcc gtcctacgaa gatctcaagc agcgcatcga cgccctactc gcggagtagt
     1381 agtgaaggtc agttcagcgg gaactgccac ccaggcgcct gcggagttca ttcaacgcgt
     1441 cacgccatgg gtcgtcacga tcacatcgat agtggccttc gcggccgcgg cggtcctgat
     1501 ggtcgagaag atcatcctgc tcggggaccc gacctacgtc ccaacttgtt cgatcaatcc
     1561 ggttctctcc tgcggctcga tcatgaaaag cgaccaggcc gaagcgttcg gctttccgaa
     1621 cccgcttctc ggccttgccg ggttcggtct gatgaccacc t
//
LOCUS       DAOQMF010000504         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55288_length_1660_cov_2.938318, whole genome shotgun sequence.
ACCESSION   DAOQMF010000504 DAOQMF010000000
VERSION     DAOQMF010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55288_length_1660_cov_2.938318"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLV77_08365"
     CDS             complement(<1..239)
                     /locus_tag="PLV77_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNC15900.1"
                     /translation="MSESASWSEYSARQITGAGLRRSVPRQRVIDLLADRNCAVTALE
                     IDAELDGVGRATVYRAIEQLEDLGLVHRVDLGGASL"
     gene            complement(214..1107)
                     /locus_tag="PLV77_08370"
     CDS             complement(214..1107)
                     /locus_tag="PLV77_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNC15901.1"
                     /translation="MIDWLIDPLTYGFTVRAVIEIAVLGAVSGAVGCWVVLYGISYSA
                     ESLAHGMFPGLVGAALLGIPLLLGGAAGIILAGLLIALVGRLTRGRPGNDDAADTAIA
                     VVVTSLFGLGVLMALSPDSPPGIQALLFGDPLGITDGQLISTAALGAAVLALLWLGHD
                     RLLAVGFDNSSGQVAGLSPGLVQAMLLVLVAVTVLIGVQGLGNLLVAAILVGPAAAAR
                     LLTSRMGSMIGISVAVAVASGIAGLYLSFHGRIAAGAAIVICLVAAWLLAALFAAVRS
                     RFRRPDSIRKELDHERIGQLV"
     gene            complement(1104..>1660)
                     /locus_tag="PLV77_08375"
     CDS             complement(1104..>1660)
                     /locus_tag="PLV77_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC15902.1"
                     /translation="ATVAQTDRSRLDYPVSALDVATMGSLARLPWYRRPGRGERRRAL
                     EALEEVGLGEQANATFGDLSGGQRQRVLIARALTADASLLLMDEPFTGLDAASEQRLE
                     ALIDGLAAAGRTVLIATHEVEQAERWDLVLCLNGEQISFGDPAVAISKPVLEATYGSH
                     IVEIPGQPELGVLPSHHHHHREER"
BASE COUNT          299 a          551 c          594 g          216 t
ORIGIN      
        1 agggaggcgc cgccgaggtc gaccctgtga acgaggccca ggtcttcgag ctgttcgatc
       61 gcacgataga cggtggcgcg gccgaccccg tcgagttcgg cgtcaatctc cagtgcggtg
      121 accgcgcagt tgcggtccgc gagcaggtcg atgacccgct gccggggaac gctgcggcgc
      181 aggccggccc cggtgatctg ccgggccgag tattcagacc agctggccga ttcgctcatg
      241 gtcgagctcc tttcggattg agtcgggccg ccggaagcgg ctccggacgg cggcgaaaag
      301 tgcggccaga agccaagcag caacgagaca gatgacgatc gcggcaccgg ctgcgatgcg
      361 cccgtggaaa gacaggtaaa ggccggcgat gccggaagcg acggcgaccg cgacgctgat
      421 tccgatcatc gatcccatgc ggctggtcag gaggcgggcg gccgccgccg ggccgaccag
      481 gatcgccgcg accagcagat tgccgagacc ctgaacgccg atcagtacgg tcacggcgac
      541 caggacgaga agcatcgcct ggaccagacc gggggagaga ccggcgacct gaccggagga
      601 gttgtcgaag ccgaccgcga gcagccggtc gtggccgagc cagaggaggg ccagcacggc
      661 ggctccgaga gccgcggtac tgatcagttg cccgtcggtg atcccgaggg ggtcgccgaa
      721 gagcagggcc tgaatcccgg gcggactgtc cggtgaaagg gccatcagca cgccgagccc
      781 gaaaagcgag gtgacgacca ccgcgatcgc cgtgtcggcg gcgtcgtcat tgccgggccg
      841 gcctctggtc agccggccga ccagggcgat cagcaacccg gccaggatga tccccgccgc
      901 accaccgagc agcaggggaa tcccgagcag ggccgcgccg accagtccgg ggaacatgcc
      961 atgggcaagc gactcggcgc tataggagat cccgtacagc acgacccagc agccaaccgc
     1021 tccgctgact gccccgagga ccgcgatctc gatgaccgcc cggaccgtga acccgtaggt
     1081 cagcgggtcg atcagccagt cgatcatctc tcctcccgat gatggtgatg atgggagggc
     1141 aggacgccga gttcgggctg tccgggaatc tcaacgatgt ggctgccgta ggtggcttcc
     1201 agcaccggct tgctgatcgc cacggccgga tcaccgaagg agatctgctc accgttcagg
     1261 cagaggacca gatcccagcg ttcggcctgc tcgacctcgt gggtggcgat cagcacggtt
     1321 ctgccggccg ccgcgagccc gtcgatcagg gcttccagcc gctgctcact ggccgcatcg
     1381 agtccggtga acggttcgtc catcagcagc aggctggcat cggcggtcag ggcgcgggcg
     1441 atcaggactc gctgacgctg gccgccggaa aggtcgccga aggtcgcgtt ggcctgctcg
     1501 cccagaccga cctcctcaag ggcctcaagg gcgcggcgcc gctcgccgcg accgggccgc
     1561 cggtaccagg gcaggcgggc cagcgaaccc atcgtcgcca catcaagcgc cgagaccgga
     1621 tagtcgagcc gggaccggtc ggtctgggcg accgtggcgg
//
LOCUS       DAOQMF010000505         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55295_length_1660_cov_2.672897, whole genome shotgun sequence.
ACCESSION   DAOQMF010000505 DAOQMF010000000
VERSION     DAOQMF010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55295_length_1660_cov_2.672897"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1660
                     /gene="carB"
                     /locus_tag="PLV77_08380"
     CDS             <1..>1660
                     /gene="carB"
                     /locus_tag="PLV77_08380"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNC15903.1"
                     /translation="PVVIGQAAEFDYSGVQACKVLLEEGYEVVLVNSNPATIMTDPEF
                     ATRTYVEPLLPGPVAAIIEKERPDALLPTLGGQTALNLAMDLSADGTLEKFGVELIGA
                     NRDAIIRGEDREVFRQVMTEAGIRIPWSITVESVEQALKELDEGDASLPAIIRPAFTM
                     GGQGGGIAETRDELIEVVSKGIAASPIGQVLVEESVIGWGEFELEVMRDRNDNVVIIC
                     SIENVDPMGVHTGDSITVAPAQSLTDPVYQSLRDIAIAVIREVGVETGGSNVQFAVNP
                     ADGEIVVIEMNPRVSRSSALASKATGFPIAKMAAKLAVGYALEEIPNDITRATPASFE
                     PTIDYVVTKIPRFAFEKFPGADEKLSTYMQSVGEVMSIGRTFRESFAKALASRELDSR
                     PEVPESDNGLLNAIATPTPGRIDLVLAAFERGLTVEQVHEQAKIDPWFLRELELLATD
                     GDGTGGLERIYNSVDTCAAEFEAATPYYYSSHEPEGSPSEIRRGEKESVVILGAGPNR
                     IGQGIEFDYCCVHAAMTARECGRDAVMVNCNPETVSTDYDTSDRL"
BASE COUNT          296 a          552 c          553 g          259 t
ORIGIN      
        1 accggtcgtg atcggacagg ctgccgagtt cgactactcc ggggtccagg catgcaaggt
       61 gctgcttgaa gagggttacg aggtggtgct ggtcaactcg aatccggcca cgatcatgac
      121 cgaccccgag ttcgcgaccc gcacctatgt cgaaccgctc ctgcccggcc cggtcgcggc
      181 gatcatcgag aaggaacggc cggacgcgct gctgcccacc ctcggcggcc agaccgcgct
      241 gaacctcgcc atggacctct ctgccgacgg caccctggag aagttcgggg tcgagctgat
      301 cggggccaac cgcgacgcga tcatccgcgg cgaggaccgc gaggtcttcc gccaggtgat
      361 gaccgaagcc ggaatccgga ttccctggtc gatcaccgtc gagtcggtcg agcaggccct
      421 gaaggaactc gacgagggtg acgcgtcgct gccggcgatc atccggccgg ccttcaccat
      481 gggcggccag ggcgggggaa tcgccgagac ccgtgacgag ctgatcgaag tcgtttcgaa
      541 gggcatcgcc gccagcccga tcggccaggt gctggtcgag gagtcggtga tcggctgggg
      601 cgagttcgaa ctcgaggtga tgcgcgaccg caacgacaac gtggtgatca tctgctcgat
      661 cgagaacgtc gaccctatgg gggtccacac cggcgattcg atcaccgtcg ctccggccca
      721 gtcgctgacc gacccggtct accagtcgct gcgggacatc gcgatcgccg tgattcgcga
      781 ggtcggggtc gagaccggtg gctccaacgt ccagttcgcg gtcaacccgg ccgacggcga
      841 aatcgtggtg atcgagatga acccgcgggt gtcgcgctcg tcggctttgg cctcgaaggc
      901 aaccggcttc ccgatcgcca agatggcggc caagctggcg gtcggctacg cgctcgagga
      961 gatcccgaac gacatcaccc gggcgacccc ggcctcattc gagccgacca tcgattacgt
     1021 ggtgaccaag atcccgcgct tcgccttcga gaagttcccc ggagccgacg agaagctctc
     1081 gacctacatg cagagcgtcg gcgaggtcat gtcgatcggg aggaccttcc gcgaatcctt
     1141 cgccaaggcg ctggcttccc gcgagctcga ttcccggccc gaagtgccgg agagcgacaa
     1201 cgggctgctg aacgcgatcg ccaccccgac cccgggccgg atcgacctgg tcctcgccgc
     1261 cttcgagcgc gggctgaccg tcgagcaggt tcacgaacag gcgaagatcg acccctggtt
     1321 cctgcgcgaa ctggaactgc tggccaccga cggcgacggc accggcggcc tcgagcggat
     1381 ctacaactcg gtcgacacct gcgcggccga gttcgaggcg gcgaccccgt actactactc
     1441 gtcgcatgag ccggaagggt caccctccga gattcgccgg ggtgagaagg aatctgtggt
     1501 gatcctcggg gccggcccga accggatcgg ccaggggatc gagttcgact actgctgcgt
     1561 ccacgccgcg atgaccgccc gcgagtgcgg acgcgacgcg gtgatggtca actgcaatcc
     1621 ggaaacggtc tcgaccgatt acgacacttc ggaccggctc
//
LOCUS       DAOQMF010000506         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55297_length_1660_cov_2.653583, whole genome shotgun sequence.
ACCESSION   DAOQMF010000506 DAOQMF010000000
VERSION     DAOQMF010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55297_length_1660_cov_2.653583"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="PLV77_08385"
     CDS             <1..1537
                     /locus_tag="PLV77_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNC15904.1"
                     /translation="LIAHDIKAVGGQRVGLLAALEATEQGPVISHDTLVAAYLLEPSR
                     RNYHLDELAVQAGLAPSGIDGDDAQMSLIADEQDSGSIAPEIAATVWNLARVQRPKLE
                     EAGLLDLLEQVEQPLIRVLAAMERIGLKLDRERVAEMAAGLEDRIIELETEIHGLAGH
                     EFTIGSPQQVGQVLFEELGLTRKRKGKTGFSTDARVLAQIRQEHAIVALIEQWRELTK
                     LKNTYLEPLPAAIDPATGRVHTTFMQVAAPTGRISSIQPNLQSIPIRSEVGRPLRGCF
                     VAEPGNLLTSLDYSQVELRVLAHVADEEVLKGFFRAGEDVHSATAAQVFEIPPEEVDE
                     AQRSKAKMVNFGIVYGLTGFGLADRLNIPRSEGEEFVKRYHERFPAVTAFREQVVEEA
                     TETGFVKTLLGRRRPLPELRSNQAQVRAQGVRLAMNTVVQGTAADIIKIAMIRSHQAL
                     LDEGLSSRLVLQIHDELLFEGPAEEAEAVTELARREMVGAFDLDPPLEVDAGSGESWL
                     AAK"
BASE COUNT          290 a          546 c          572 g          252 t
ORIGIN      
        1 gttgatagcg catgacatca aggcagtcgg gggccagcgg gttgggttgc tggcggcgct
       61 cgaagcgacc gaacaggggc cggtgatcag tcacgacaca ctggtcgccg cctatctgct
      121 cgaaccctcg aggcggaact accacctcga cgaactcgcg gtgcaggccg gtctggcacc
      181 ttccggcatc gacggtgacg acgcccagat gtcgctgatc gccgacgaac aggactccgg
      241 atcgatcgcc ccggagatcg ccgccacggt ttggaacctg gcccgggtcc agcggccgaa
      301 gctcgaggag gccggtctgc tcgatctgct ggagcaggtg gaacagccct tgatccgggt
      361 cctcgcggcc atggagcgga tcgggctgaa actcgatcgt gaacgggtcg ccgaaatggc
      421 cgccggtctc gaggaccgga taatcgagct ggaaaccgag atccacggac tggccggcca
      481 cgagttcacg atcggctcgc cgcagcaggt gggccaggtg ctcttcgagg agctgggcct
      541 gacccgcaag cggaagggca agaccggttt ctcgaccgac gcaagggtgc tggcccagat
      601 ccggcaggaa cacgcgatcg tcgccctgat cgagcagtgg cgcgagctga ccaagctcaa
      661 gaacacctac ctggagccgc tgccggcggc aatcgacccg gcgaccgggc gcgttcacac
      721 gaccttcatg caggtcgccg ccccgaccgg gcggatctcc tcgatccagc ccaatctgca
      781 gagcatcccg atccgttccg aggtcggccg cccgctccgg ggctgcttcg tcgccgagcc
      841 gggcaacctg ctcacctcgc tcgactacag ccaggtggag ctcagggtgc tggcccatgt
      901 cgccgacgag gaagtgctga agggattctt ccgggccggg gaggatgtcc actcggcgac
      961 cgcggcccag gtcttcgaga ttcccccgga agaggtcgac gaggcgcagc ggtcgaaggc
     1021 aaagatggtc aacttcggca tcgtctacgg cctgaccggc ttcggcctgg ccgaccgcct
     1081 gaacatcccc cgctcggaag gggaggaatt cgtcaagcgc taccacgaaa ggttcccggc
     1141 ggtcaccgcc ttccgggagc aggtggtgga ggaggcgacc gagaccggtt tcgtcaagac
     1201 cctgctcggc cggcggcgac cgttgccgga gttgcgttca aaccaggccc aggtccgggc
     1261 ccagggggtg cggctggcga tgaacaccgt ggtgcagggc accgcggccg acattatcaa
     1321 gatcgcgatg atccgcagcc accaggcgct tctcgacgaa ggtctctcga gccgcctcgt
     1381 gctgcagatc cacgacgaac tgcttttcga aggcccggcg gaggaagccg aagcggtcac
     1441 cgaactggcc cggcgggaaa tggtcggcgc cttcgacctt gacccgccgc tggaggtcga
     1501 cgccggctcc ggcgagagct ggctcgcggc caagtagcgg cagcccggat tccggcatga
     1561 gcgattccct ggtcgtcctg ctgatggtcc tggtcggggt ccagtttgcc gcccaggccc
     1621 cggtcaacgg cgggctcggc cgccacactg gcgggctggt
//
LOCUS       DAOQMF010000507         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55351_length_1659_cov_3.104738, whole genome shotgun sequence.
ACCESSION   DAOQMF010000507 DAOQMF010000000
VERSION     DAOQMF010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55351_length_1659_cov_3.104738"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLV77_08390"
     CDS             complement(<1..723)
                     /locus_tag="PLV77_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15905.1"
                     /translation="MVRRPLPLAFLLLLLFLAPPARAADFPAGIEAGALTDPPLIASN
                     CERIREDGVGTSVINAVMTRIVKSPGVYDFASIDSQVNAAISCGIDPAMRVFAEPSSS
                     DERGMPDDLDAYGAFFGALVGHLKGRVGRFGVENEVISTTHWTDTPAGYFQLLRVAAD
                     ATHAANPDAIILDSVSSSGGFSIIEARKQWLAGDLEGALATIQESQTNDLGGGPYLTS
                     TSQLPAYLSNAKVMKMQEFYDQL"
     gene            complement(771..>1659)
                     /locus_tag="PLV77_08395"
     CDS             complement(771..>1659)
                     /locus_tag="PLV77_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC15906.1"
                     /translation="RVGYEQVSEAFGPGAAGTLQVVAPGDDATRALASLRNDQGIARV
                     MVAGESPGGYTLIAATPGVDPSDPAMGTTIDRVRDALPPGTLVGGAAAENHDLEAALG
                     DTTAPAIAIVMTLGFLLLLIALRAPILAAIAVLTNLLAVGAAFGVAQLIFQQGHLTGL
                     LGIESQGFLDAWAPVFFFAMVFAISMDYSVFLLSSAREHWEKSGDAKEALIGALAHSG
                     RVVFAAAGVMVAVFFTFALSGPLPPREMGIILGVAVLLDAGLVRLMLLPVLMRLAGPG
                     LWWIPGWMDRRLPRVSFGH"
BASE COUNT          305 a          538 c          574 g          242 t
ORIGIN      
        1 cagctggtcg tagaactcct gcatcttcat gaccttggcg tttgaaaggt aggccggcag
       61 ctggctggtc gaagtgaggt aggggccgcc gccgaggtca ttggtctggc tttcctgaat
      121 cgtggccagg gcaccttcga gatcgccggc cagccactgc ttgcgggcct cgatgatcga
      181 aaagccgccg ctcgagctga ccgaatcgag gatgatcgcg tcggggttgg ccgcatgggt
      241 cgcgtcggcc gcgacccgga gcaactggaa atacccggcg ggagtgtcgg tccagtgggt
      301 cgtcgagata acttcgtttt cgactccgaa acgacctacc cgccccttca ggtgaccgac
      361 cagggcaccg aagaacgcac cgtaggcatc gaggtcatcc ggcatgccgc gctcgtcgct
      421 gctggagggt tcggcgaaga cccgcatcgc cgggtcgatt ccgcaggaga tcgcggcgtt
      481 gacctgggag tcaatcgatg cgaagtcgta aaccccgggg gatttcacga tccgggtcat
      541 gaccgcgttg atcaccgaag tcccgacgcc gtcttccctg attcgctcgc agttcgaggc
      601 gatcagcggc gggtccgtca gcgctccggc ctcgatcccg gcggggaagt cggcggcccg
      661 cgccggcggg gccaggaaca ggaggagcag caggaacgcc agggggagtg gacgccggac
      721 cattaccttg ggaacccggc agccggtggc cggttgcgct cctgcccgga tcagtgaccg
      781 aaactgaccc gaggcaggcg cctgtccatc cagcccggaa tccaccagag cccgggcccg
      841 gcgagccgca tcaggaccgg cagcagcatc aacctgacca gacccgcgtc cagcagcacg
      901 gcgacaccga ggatgatgcc catctcgcgc gggggcaggg ggccggagag cgcgaaggtg
      961 aagaacacgg cgaccatgac gccggcagcc gcgaacacaa cccgtccgga atgggccagc
     1021 gcaccgatca gggcctcctt cgcgtcaccg gacttttccc agtgttcgcg ggccgagctg
     1081 agcaggaaca cggaatagtc catcgagatc gcgaagacca tcgcgaaaaa gaagaccggt
     1141 gcccaggcgt cgaggaagcc ctgggattcg atgcccagca gcccggtcag gtgaccctgc
     1201 tggaagatca gctgggccac gccgaaggcg gcaccgaccg ccagcaggtt ggtcagcacc
     1261 gcgatcgcgg ccaggatcgg ggctcgcagc gcgatcagca gcaggaggaa gccgagggtc
     1321 atgacgatcg cgatcgccgg cgcggtggtg tcacccaaag cagcttcgag gtcatggttc
     1381 tcggccgcgg ctccgccgac cagggtgccg ggcggcaatg cgtcacggac ccggtcgatc
     1441 gtcgttccca ttgccggatc ggatggatcc acgcccggag tcgccgcgat cagggtgtag
     1501 ccaccgggac tttcgccggc caccatgacc cgggcgatcc cctgatcgtt ccggagggag
     1561 gcgagcgccc tggtggcgtc gtcaccgggc gcgactacct gcagggttcc ggccgccccg
     1621 ggaccaaagg cttcggagac ctgttcgtag ccgacccgg
//
LOCUS       DAOQMF010000508         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55878_length_1651_cov_3.609649, whole genome shotgun sequence.
ACCESSION   DAOQMF010000508 DAOQMF010000000
VERSION     DAOQMF010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55878_length_1651_cov_3.609649"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..869
                     /locus_tag="PLV77_08400"
     CDS             387..869
                     /locus_tag="PLV77_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15907.1"
                     /translation="MKTRLTALVLSLATLFSGVLMPVSASAAGWGRGTFTGKVRGQFR
                     PQPLTSVAIAARGDKVRIVKLGIAFDCSDNNQFGLPLHWKTLVYSKAVRVKEGPAGGG
                     AIIHFRVVKNYRGKKVPVRGDIFLGLRDTYIRGTVDSEVARGWNPQDCSDSGIFIARR
                     "
     gene            complement(887..>1651)
                     /locus_tag="PLV77_08405"
     CDS             complement(887..>1651)
                     /locus_tag="PLV77_08405"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC15908.1"
                     /translation="RQTRDELLDLLPDGYTLEGRSLLDFGCGAGRTLRQFHEEASSAR
                     VVGCDIDRESIDWMQANLAPIEAVCSEVDPPLPFESGSFDFIWAISVFTHLTGNSAAW
                     LMELHRLLKPGGLLMASYMGELNSEEVAGEPWDENRIGMNVLRHSQGWDVGGPMVLMS
                     DWWVDEHWGRAFEIRKRHFAGGQTWALLEKKDVRLSADELMAAGDDPREIAALRHNVA
                     QLQAEIDDLKSHQQGGSLARAGSALKRRASRLRRPD"
BASE COUNT          287 a          550 c          525 g          289 t
ORIGIN      
        1 ttcccggctt attcctcgcc agccacccgc aggagcgccc tgtgcacgac ccgctcaagc
       61 caagtggtgt tggcatcctc gagatctggt ttttcggcgg atagcgcacc caattccgat
      121 ctcgatcgca acctgacccg atccggggga ggtgggtatg gatttcgtcc atatagacga
      181 aatccatacc cacctcccga aacccgggcc tgaaccgggc cccggaggtg ccacaggact
      241 cgggaacacc ctcaactccc ccttcaatgc cgctgtttat cggcagtggg tcggataacc
      301 ggcaatcggg ccggaaatcc gacccacgag acttgcgaag ccgggttcgc aaggtggtgg
      361 caaggtgggg tttctaggtt gggagcatga agacccgact cacagccctc gtcctgtccc
      421 tggccaccct cttttccggc gtcctgatgc ccgtttcggc ttccgcagcc ggctggggcc
      481 gcggcacctt caccggcaag gtgcggggtc agttccggcc tcagccgctg accagcgtgg
      541 ccattgccgc gcggggtgac aaggtgcgga tcgtcaagct cgggatcgcc ttcgactgct
      601 cggacaacaa tcagttcggc ctgccgcttc actggaagac actcgtctac agcaaggcgg
      661 tgcgggtaaa ggaagggccg gccggaggcg gggcgatcat ccacttccgg gtggtcaaga
      721 actaccgggg caagaaagtc ccggtcaggg gcgacatctt cctcggcctg cgcgatacct
      781 acatccgggg cacggtcgat tcagaggttg cccgcggctg gaatccccag gactgctcgg
      841 acagcggcat cttcatcgcc cgcaggtagg acgccccggc gccggctcag tccgggcgac
      901 gcaaacggct ggcccttcgc ttcagggctg aaccggcccg ggcgagtgac ccgccctgct
      961 gatgggactt gaggtcgtcg atctccgcct ggagctgggc gacgttgtgg cggagggcgg
     1021 cgatctcacg gggatcgtcg ccggccgcca tcagttcatc ggcggaaagc cggacatcct
     1081 tcttttccag aagggcccag gtctgacctc ccgcgaagtg ccgtttgcgg atctcgaagg
     1141 cccggcccca gtgctcgtca acccaccagt cggacatcaa gaccatcgga ccgcccacat
     1201 cccatccctg gctgtgacga agcacgttca tgccgatccg gttctcgtcc cacggctcgc
     1261 cggcgacctc ttccgagttg agctcgccca tgtaggaggc catgagcaga ccgcccggct
     1321 tgagcaggcg gtggagttcc atcagccagg cggccgagtt gccggtcagg tgggtgaaca
     1381 ccgagatcgc ccagatgaag tcgaagcttc cggactcgaa gggcaggggc ggatcgactt
     1441 cggagcagac cgcctcgatc ggcgccaggt tcgcctgcat ccagtcgatg ctctcgcggt
     1501 cgatgtcgca tccgaccacc cgcgccgagg aagcctcctc gtggaattgc cgcagggtgc
     1561 ggccggcgcc gcaaccgaag tcgagcaggc tgcgaccttc cagtgtgtat ccgtcgggca
     1621 gcagatccag cagctcatcc cgcgtctgcc g
//
LOCUS       DAOQMF010000509         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_55888_length_1651_cov_2.961153, whole genome shotgun sequence.
ACCESSION   DAOQMF010000509 DAOQMF010000000
VERSION     DAOQMF010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55888_length_1651_cov_2.961153"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLV77_08410"
     CDS             <1..480
                     /locus_tag="PLV77_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15909.1"
                     /translation="VAVYSARAMDGVSEDFSGLPEYKTRSERNAERVDRRFGQRRNNP
                     YRGHRTRIILVGGFAVALVLLALSLVVLFAPTPYLGTNYASIAKSVGAASAHDCRPGG
                     SGWVCTKEVDGSAARYEVKVDWAGCWDGHLIGKPAGSNGAQPEISGCVSLMDHLTAG"
     gene            complement(467..1135)
                     /locus_tag="PLV77_08415"
     CDS             complement(467..1135)
                     /locus_tag="PLV77_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15910.1"
                     /translation="MAFFAALAAVLLVPSAAEAQTWSGGGITLPGPTTNPNIGKILPL
                     ARREFSRNVVERRGNNVPRYRNGKGRIAPYSISDQWCAAFSTWIWNHAGFKSYLGTSI
                     LWPSYDGTMVGVQVRDLTNWATRNGYFSYRAKPGYLVAYGTTHIGIVEKIDRTTGRAV
                     GSIEGNIGNRVNRVTVPMDRVTGYISPVRLTPIQLDKSAAYADMAVPPAALERAEASG
                     FSQP"
     gene            complement(1265..>1651)
                     /locus_tag="PLV77_08420"
     CDS             complement(1265..>1651)
                     /locus_tag="PLV77_08420"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HNC15911.1"
                     /translation="DASGEAALVFFWAPQDRQVRVRGPVRRLDADASDRYFASRDRAS
                     QVGAWASPQSQPIPDQREALEELAEAAADRFAEGEVPRPPHWGGFILEPRSIEFWQGR
                     GARLHDRFRYDRQASGEGWKITRLAP"
BASE COUNT          273 a          542 c          559 g          277 t
ORIGIN      
        1 gtggcggtct actctgctcg cgcgatggat ggtgtgagcg aagatttcag cgggctgccc
       61 gaatacaaga cccggagcga gcggaatgcc gaacgcgtcg atcgccggtt cgggcagcgc
      121 cggaacaacc cttatcgcgg tcaccggacc aggatcatcc tggtcggtgg tttcgcggtc
      181 gccctggtcc tgctggccct ctcgctggtc gtcctcttcg cgccgactcc gtacctcggc
      241 accaactacg cctcgatcgc caagtcggtc ggggccgcat ccgcccacga ctgcaggccc
      301 ggcggaagcg gctgggtctg caccaaagag gtcgacggca gcgcggcccg ttacgaggta
      361 aaggtcgact gggcgggttg ctgggatggc cacctgatcg gcaagcccgc cggcagcaac
      421 ggcgcgcaac cggagatctc cggctgcgtg tcattgatgg atcaccttac ggctggctga
      481 acccggaagc ttcggcccgc tcgagcgcag ccggcgggac cgccatgtcg gcgtaggcgg
      541 cactcttgtc gagctggatc ggggtcagcc gcaccgggct gatgtatccg gtgacccggt
      601 ccatcggcac ggtcacccgg tttacccggt tgccgatgtt gccctcgatc gagccgaccg
      661 cgcgaccggt ggtccggtcg atcttctcga cgatgccgat gtgggtggtt ccgtaagcga
      721 cgaggtagcc cggtttcgcc cggtaggaga agtagccgtt cctggtcgcc cagttggtca
      781 ggtcccggac ctggaccccg accatggtgc cgtcatatga cggccagagg atgctcgtcc
      841 cgaggtagga cttgaagccg gcgtggttcc agatccaggt cgagaaggcg gcgcaccact
      901 gatccgagat gctgtacggc gcaatcctgc ccttgccgtt tcggtagcgg ggaacgttgt
      961 tgccgcggcg ttcgaccacg ttgcgggaaa actcacgccg ggccagcggc aggatcttgc
     1021 cgatgttcgg gttggtggtc ggaccgggca gcgtgatgcc tccgccggac caggtctggg
     1081 cttcagccgc cgatggaacc agaaggactg cggcgagggc ggcgaaaaac gccaaccgga
     1141 cttttaacgg aaaaacggac atcacccctg aggcttgcag gctaaacagg ttcggggcag
     1201 gttgtccagc cccctgcgag atgtcatgca gccggtgacc gcgaaagaac cggacccttt
     1261 tcgctcaggg agccaggcgg gtgatcttcc agccctctcc cgaagcctgg cggtcgtagc
     1321 gaaaacggtc gtggagccgg gccccgcgac cctgccagaa ctcgatcgag cggggctcca
     1381 ggatgaaacc tccccaatgc gggggtcgcg gtacttcacc ttccgcgaaa cgatcggccg
     1441 ccgcttcggc cagctcttcg agcgcctccc tctggtcggg aatcggctgg ctctgggggg
     1501 aagcccaggc gccgacctgg ctcgcccggt cccgactcgc gaaataccgg tcagaggcat
     1561 ccgcgtccag ccgtcgcacc gggccccgca cccggacctg ccggtcctgc ggcgcccaga
     1621 agaagaccag ggcggcctcc ccgctggcat c
//
LOCUS       DAOQMF010000510         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_56079_length_1648_cov_3.387947, whole genome shotgun sequence.
ACCESSION   DAOQMF010000510 DAOQMF010000000
VERSION     DAOQMF010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56079_length_1648_cov_3.387947"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..>1648
                     /locus_tag="PLV77_08425"
     CDS             309..>1648
                     /locus_tag="PLV77_08425"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC15912.1"
                     /translation="MGYETRCLIGGEFVEGEGASIEVENPASEEIVATVGSASLEQLD
                     LAVAKATEAKASWGGMPAVDRAPLLHGIAAALREKTDELALLMTTEIGRPLSESADEV
                     EWCAACFDYYAEVARSEIGRVIPPIEASQFSVVVKEPHGVVGCIVPWNFPLLLLVWKV
                     APALAAGNTIVAKPSELTPLSTLAIAEVFSELPDGTVNLLAGAGDVGAGISGHPGIDC
                     VAFTGSVATGRKVGVAAASRNARVNLEMGGKDPFIVCDDIGTDGLEVAARGGAWAAFL
                     NAGQVCTSSERFYVMESVFDEYVSAFVEHTRSLVIGDPLESATDLGPMVSATQRGKAL
                     GQVEDAVAGGAELLTGGGNAGFGKGHYLEPCVLTGVDAAADLLREETFGPVAPIVPVK
                     DLDEAIALANGLDFGLGANIYTRDLKRAIRCVKEVRAGSVWINDPLTDNDAGPFG"
BASE COUNT          257 a          539 c          560 g          292 t
ORIGIN      
        1 cccggatcga aatcggagtt gggtgcgcta tttgccgata actcccgcct cgaatccccc
       61 ccgagattcc aacccgctcc cccttcacca gcccctgccg gcttcaaggt gacgcaggtt
      121 tgcgacctct ccggtggcaa acctgcggca ccttcgaaaa acacacccgg cacgtctcga
      181 ggtgccgcgc attcaggacc gttacgggct taaacgtgcg gcacctgcga gcaacccagg
      241 tgttgcaggt ccggcgggtt gaagctggaa gcggggcgaa gcttgccggc tgcagttagt
      301 tttcccgcat gggatatgag actcgttgtc tgattggtgg agagttcgtt gagggagaag
      361 gcgcctcgat cgaggttgag aacccggctt ccgaggagat cgtcgcgacc gttggttcgg
      421 cttcgctgga gcagcttgac ctcgccgtcg ccaaggcgac cgaggcgaag gccagctggg
      481 gcgggatgcc ggccgttgac cgggctcctc tgctgcacgg gatcgccgcg gccctgcgcg
      541 agaagaccga cgaactcgcg ctgctgatga ccaccgagat cggccggccg ctttccgagt
      601 cggcggacga ggtcgaatgg tgcgcggcct gcttcgacta ctacgcggag gtcgcccgtt
      661 cggagatcgg acgcgtgatc ccgccgatcg aggcctcgca gttctcggtc gtggtcaagg
      721 agccacacgg ggtggtcggc tgcatcgtgc cgtggaactt ccccctcctc ttgctggtct
      781 ggaaggtggc cccggccctg gccgcgggca acacgatcgt ggccaagccc tccgagctga
      841 ctcccctctc gaccctcgcg atcgccgagg tcttttcgga gctgccggac ggcacggtga
      901 acctgctggc gggagctggc gatgtcggcg ccgggatctc cggccatccc gggatcgact
      961 gcgtcgcatt taccggttcg gttgccaccg gccggaaggt cggcgtcgcg gctgcttccc
     1021 gcaatgcccg ggtcaacctc gagatgggcg gcaaggaccc gttcatcgtc tgcgatgaca
     1081 tcggcaccga cgggctcgag gttgccgccc gtggcggtgc ctgggcggcc tttctgaacg
     1141 ccggccaggt ctgcacctca tccgagcgct tctacgtgat ggagtcggtc ttcgacgagt
     1201 acgtgagcgc cttcgtcgag cacaccaggt cgctagtgat cggcgatccg ctggagtcgg
     1261 cgaccgacct cgggccgatg gtctccgcca cccagcgcgg caaggcgctg ggccaggtcg
     1321 aggacgctgt cgccggggga gccgaactgc tcaccggtgg aggaaatgcc ggattcggga
     1381 agggccatta cctcgagccc tgtgtgctga ccggggttga cgccgcggcc gatctgctcc
     1441 gcgaagagac tttcgggccg gtcgcgccga tcgtgccggt caaggacctc gacgaggcga
     1501 tcgcgctcgc caacggcctc gacttcggcc tcggcgcgaa catctacacc cgcgacctta
     1561 agcgggcgat ccgctgcgtg aaggaggtcc gggcaggttc ggtctggatc aacgaccccc
     1621 tgaccgacaa tgacgccgga cccttcgg
//
LOCUS       DAOQMF010000511         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_56401_length_1643_cov_2.840050, whole genome shotgun sequence.
ACCESSION   DAOQMF010000511 DAOQMF010000000
VERSION     DAOQMF010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56401_length_1643_cov_2.840050"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLV77_08430"
     CDS             complement(<1..246)
                     /locus_tag="PLV77_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15913.1"
                     /translation="MSWPDPGILDRELTRLSGVGAKLGAAAAKAGVGTIGDLLWRLPR
                     AYGRRPGTSLLGDLEPGEATGVEVRVLRSRRVRTRRRR"
     gene            complement(336..>1643)
                     /locus_tag="PLV77_08435"
     CDS             complement(336..>1643)
                     /locus_tag="PLV77_08435"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNC15914.1"
                     /translation="VDPVLVASAFGKAADAAYASVRNPAEGTMLTVFREIAHSVSRQL
                     AHIEPEQQRLAKDATPAEQDQVLADVLEQAIADGQKAVERTPEQLEVLAQSGVVDAGG
                     YGLVLILAGVLAALRGEDADLPEIQHHSAAQITHPEHEDSRFQYCTNFIVSGSGLNSR
                     QFIGRLEEIGDSVLVVGDESTLKVHVHTDDPEAAMEVFVEAGAVTNLDVADMRKQIAE
                     REARINADHRTGVVVVGSGDGLESLFTEMGATVVAGGETMNPSTAELLAGIRAVPVEE
                     VLVLPNSSNVIMAAERACELSEKRAVVLPTRTQQEGLLALIEFNGESDLDTNRRRLEE
                     AVAEVTVGGIAPAARDDAQGRFRRGDAVGFISGEIVAWGGAGSALASTIERLADEAEL
                     VTVIAGEGAPIPLDEIDDHSPNGVELELHEGGQPSWWWLLASQ"
BASE COUNT          235 a          588 c          544 g          276 t
ORIGIN      
        1 cctgcgccgt ctggttctga cccggcggga cctcagcacc cggacctcca ccccggtcgc
       61 ttcacccggt tcgagatccc cgagcagact cgttccgggg cgccgcccgt aggcgcgcgg
      121 gagccgccag agcaggtcgc cgatcgtccc gaccccggcc ttggcggccg ccgccccgag
      181 tttcgcgccg accccgctca accgggtcag ttcccggtcc agaatgccgg ggtcaggcca
      241 gctcacgggg gcggcgagca agctttttcg gtccgggtcc ggttccggac cgccgccgaa
      301 tccgggcgga ggcaaaggcg gcccggaagc cgcggtcact ggctggccag cagccaccac
      361 cagctcggct gcccgccctc gtgaagttcg agttcaactc cgttcgggct gtggtcgtcg
      421 atctcgtcca gcgggatcgg tgccccttcg ccggcaatca ccgtgaccag ttcggcctcg
      481 tcggcgaggc gttcgatggt tgaggccagc gccgatcccg cgccgcccca ggcgacgatc
      541 tcgccggaga tgaaaccgac cgcgtctccg cgccggaacc tgccctgtgc gtcatccctg
      601 gccgccggag cgatcccccc gaccgtaacc tcggcgacgg cttcttccag ccggcggcga
      661 ttggtgtcga gatctgactc gccgttgaat tcgatcaggg ccagcaggcc ctcctgctgg
      721 gtgcgggtcg gcagcaccac cgcccgcttc tcggagagct cgcaggccct ttcggcggcc
      781 atgatcacgt tcgaggagtt cggcaggacc agcacttcct cgaccggcac cgcccggatg
      841 ccggccagaa gttcggccgt cgaagggttc atcgtttcgc cgccggccac cacggtcgcg
      901 cccatctcgg tgaacagcga ttccagcccg tcgccgcttc cgaccacgac caccccggtc
      961 cggtgatcgg cgtttatccg cgcctcgcgt tcggcgatct gcttgcgcat gtcggcaacg
     1021 tcgaggttgg tgaccgcacc cgcctcgacg aagacctcca tcgccgcttc gggatcgtcg
     1081 gtgtggacgt ggaccttcag ggtggattca tcgccgacga ccaggaccga atcaccgatc
     1141 tcctcgagtc ggccgatgaa ctggcgcgag ttgaggccgc tgccggaaac gatgaagttc
     1201 gtgcagtact ggaagcggct gtcttcatgc tcgggatggg tgatctgggc agccgagtga
     1261 tgctggatct cgggcaggtc ggcatcctcg ccccggagcg cggccagcac accggccagg
     1321 atcaggacca gtccgtagcc gccggcatcg accacccctg actgggccag gacttcgagc
     1381 tgctccgggg tccgctcgac tgccttctgc ccgtcggcga tcgcctgttc gaggacgtcg
     1441 gcgagcacct ggtcctgctc ggccggggtc gcgtccttgg ccagtcgctg ctgttccggc
     1501 tcgatgtggg cgagctgccg cgaaaccgaa tgggcgatct cgcgaaagac cgtgagcatg
     1561 gttccctcgg ccgggttgcg gaccgacgcg taggcagcgt ccgcggcctt gccgaaggcg
     1621 gatgcgacca gaaccggatc gac
//
LOCUS       DAOQMF010000512         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_56528_length_1641_cov_3.600883, whole genome shotgun sequence.
ACCESSION   DAOQMF010000512 DAOQMF010000000
VERSION     DAOQMF010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56528_length_1641_cov_3.600883"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..609
                     /locus_tag="PLV77_08440"
     CDS             103..609
                     /locus_tag="PLV77_08440"
                     /inference="COORDINATES: protein motif:HMM:NF025683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HNC15915.1"
                     /translation="MERTAYARLYATVTGAFLVLLGFVSLLSNTEFQARELTSELLGF
                     YAINGWSGVFHVAAGLLGLLLARPLPRLYALLAGIVFTGLGIWGILAANGTWLLGGLP
                     ANRWANLVNLLIGLLGIIAYAASRWDRITAFTGSLGSRFEKMAENRRQKRRRRKIRKR
                     RTATKSRA"
     gene            complement(642..923)
                     /locus_tag="PLV77_08445"
     CDS             complement(642..923)
                     /locus_tag="PLV77_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15916.1"
                     /translation="MDEIRIENLLADALKPVEPPQRMSDRLHDTFSAITDAAASDLSD
                     WAEELTESELESLRDPRNWIRPVTALAAGGVATGALILVGFRRRSRYRG"
     gene            complement(916..1482)
                     /locus_tag="PLV77_08450"
     CDS             complement(916..1482)
                     /locus_tag="PLV77_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC15917.1"
                     /translation="MSGPDAKSTPESKAEIDRAFEELYRDHLKDVYSYCYYRIGNHHD
                     AEDLTEQTFLQAYRHFARARRESDGRPLRPWLIRIAHNLAANYYRDRSRKPQTPLESI
                     EPPAAKHSTEDLVEGRAELRAVIENLQDLPDDRREALVMRFALGMDNREIARAMGKSD
                     GATKVLIHRAIKQLQESLEAEGVSVEHG"
BASE COUNT          270 a          536 c          542 g          293 t
ORIGIN      
        1 tgaaacgcct gtctcgacgt tcgggtggcg gtaacaggta gccaaggccg tcaaacccta
       61 tcggtgtgcc cgggtccggc gttcctgtga gaatggcggt cgatggagag gacggcatac
      121 gcccggctgt acgcgacggt gaccggcgcc ttcctcgtgc tgcttggttt cgtcagcctc
      181 ctcagcaaca ccgagtttca ggcccgcgag ctgaccagcg agctgttggg cttctacgcg
      241 atcaacggct ggtccggtgt ctttcacgtc gccgccggac tactcggcct gctgctcgcc
      301 cgcccccttc ctcgcctcta cgcgctgctg gccggaatcg tctttaccgg ccttggcatc
      361 tggggcatcc tcgccgccaa cggaacctgg ctgctcggtg ggctgccagc caaccggtgg
      421 gcaaacctgg tcaacctgct gatcggcctg ctcggcatca tcgcctacgc ggccagccgc
      481 tgggaccgga tcaccgcgtt caccggcagc ctcggaagcc ggttcgaaaa gatggcggaa
      541 aatcgccgcc agaagcgcag gcgacgcaag atccgcaaac gccggacagc aaccaagagc
      601 cgcgcctagc cgcggaagcg gctggtcaca agggtgatgt gtcagccgcg gtagcggctt
      661 cgacggcgga agccgaccag gatcagggcg ccggtagcta ccccgcccgc ggcgagggcg
      721 gtgaccggac ggatccagtt gcgggggtca cgcagcgact cgagttcact ttcggtcagt
      781 tcttcggccc aatcggaaag gtccgaagcg gcggcgtcgg tgatcgccga gaaggtgtcg
      841 tgaagtcgat cagacatgcg ctgtggcggc tcgaccggct tcagcgcatc ggcgagcagg
      901 ttctcgatcc tgatctcatc catgctcaac ggaaactcct tccgcttcca gcgattcctg
      961 cagctgcttg atcgcgcgat gaatcagcac cttggtggcc ccgtcggatt tgcccatcgc
     1021 gcgggcgatt tcgcggttgt ccatgccgag cgcgaaacgc atgaccagag cctcgcgacg
     1081 atcgtctggc agatcctgaa ggttctcgat caccgcccgg agttcggccc gaccctcgac
     1141 cagatcctcg gtcgagtgct tcgcggccgg cggctcgatg ctctccagcg gagtctgcgg
     1201 tttccgcgaa cgatcgcggt agtagttcgc agcgaggttg tgggcgatcc ggatcagcca
     1261 ggggcgaagc gggcgaccgt ccgattcccg ccgggcccgg gcaaagtgcc ggtaggcctg
     1321 aagaaatgtc tgctcggtca gatcctcggc gtcgtggtga ttgccgatcc ggtagtagca
     1381 gtaggagtag acgtccttga ggtgatcgcg gtagagctcc tcgaaggccc gatcgatctc
     1441 ggccttcgac tccggagttg atttcgcgtc ggggccggac acgcccgcac cttatcccga
     1501 aaccggcgac ctccgacccg ggcgtgctca tgccgcccgc ctcgaatgcc cggagcggcg
     1561 gagcgccgca acccattcat cggagctgat tcgcccggtg gcgagatccg cgaggccggt
     1621 cagggcctcg gcggggcagc c
//
LOCUS       DAOQMF010000513         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_56757_length_1638_cov_2.535060, whole genome shotgun sequence.
ACCESSION   DAOQMF010000513 DAOQMF010000000
VERSION     DAOQMF010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56757_length_1638_cov_2.535060"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PLV77_08455"
     CDS             complement(<1..1388)
                     /locus_tag="PLV77_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15918.1"
                     /translation="MLRPGAALLAAITCLATLALTVPATSAADDAPRAENTAILVLIP
                     QGPGSPPSYSEEIADGFAGIPQLSVGLTSATQGSYRREQALLDISQGTRVSRSTYDPS
                     EVPPVGLAATVGGGGRVKGWDAILDRAETAPQTIEPGLLASSIPGGAAYVGNATRFQE
                     TVIPATNRVGRVSALASAGIGDAVHRAISQSRKYRLVVVAASPGREGLRELRRILQLR
                     RPGQLVIATQTPPLAPILPLLPIAAAGLGENPGDPIQTEGKGLRSDTTNLPNLVAGID
                     IAPTILDHLGLSIPDEMTGKPFRAEGSRDPESLTPFRDRLDELGSRRNAALAFVFVGW
                     LIIYLAAGAIAGREKIAHRVRRIGGLSILWIPTVILVPAALGNPSAAVEYLLIAGLAL
                     TLGFLSDRFVPWPRATLVPAVVGLSVITIDLVFGSHLITRSILGPNPGYGSRFYGIGN
                     ELKPALMVLLLAG"
     gene            1453..>1638
                     /locus_tag="PLV77_08460"
     CDS             1453..>1638
                     /locus_tag="PLV77_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15919.1"
                     /translation="MSNEPEQTPSARIGALAQELMEQVERDTPGARLDQLVISAVLSE
                     DAGPDEQTMHVQLVSDSS"
BASE COUNT          272 a          511 c          621 g          234 t
ORIGIN      
        1 ccggccagca gcagcaccat cagggccggt ttgagttcgt tgccgatccc gtagaaacgc
       61 gatccgtaac ccgggttcgg cccgaggatc gacctggtga tcaggtggga accgaagacc
      121 aggtcgatcg tgatcacgct caagccgaca accgccggga ccagcgttgc ccggggccag
      181 ggaacgaagc ggtccgagag gaagccgagc gtgagggcga ggcccgcgat cagcagatac
      241 tcaactgcgg ccgacgggtt gccgagggcg gctggaacca ggatcactgt cgggatccag
      301 aggatcgaga ggccgccgat ccgccgtacc cgatgggcga tcttctcgcg cccggcgatc
      361 gcgccagcgg caaggtagat gatcagccag ccgacgaaga cgaaggcgag cgccgcgttg
      421 cggcgcgacc cgagttcgtc gagccggtca cggaaggggg tgagcgattc ggggtcccgc
      481 gacccttcgg cccggaacgg cttgccggtc atctcgtccg ggatcgacag cccgaggtgg
      541 tcgaggatgg tcggggcgat gtcgatcccg gcgaccaggt tcggcaggtt ggtcgtatcg
      601 gagcgcaggc ccttgccttc ggtctggatc ggatcgccgg ggttctcgcc gaggccggcc
      661 gcggcgatcg gaagcaacgg caggatcggg gccaggggcg gggtctgggt cgcgatcacc
      721 agttggccgg gacggcgcaa ctggaggatc cggcgcagct cgcgcagtcc ttcgcgcccg
      781 ggggaagcgg cgaccacgac cagccggtac ttgcgtgact ggctgatcgc ccggtggacc
      841 gcatcgccga tgcctgccga ggccagcgcg gacacccggc cgacgcggtt ggtggccggg
      901 atcaccgtct cctggaacct ggtcgcgttt ccgacgtagg cggcaccacc gggaatcgaa
      961 ctggcgagca gtcccggttc gattgtctgg ggcgcggtct cggcccgatc gaggatcgcg
     1021 tcccatccct tgaccctgcc gcccccgccg accgtcgcag cgaggccgac cggcggcact
     1081 tccgacgggt cgtaggtcga gcgggaaacc ctggtgccct ggctgatgtc aagcagcgcc
     1141 tgctccctgc ggtaggaacc ctgggtggcc gaggtcagcc cgaccgagag ctgcgggatc
     1201 ccggcgaagc catcggcgat ctcctcgcta taggagggag gggatccggg cccctggggg
     1261 atcaggacga ggatcgccgt gttctccgcg cggggggcat catccgctgc gctcgtggca
     1321 gggacggtca gggccagggt ggccaggcag gtgatcgccg cgaggagggc ggcgccgggg
     1381 cgaagcacag cgccagttta ctttgagaat tgcggggatt agctgtggcg cgccgggcgg
     1441 tagggtggct gcatgagcaa tgaaccggag cagacgccgt cggcacgaat cggggcgctg
     1501 gcccaggaac tgatggagca ggtggagagg gacacccccg gggcacgact ggaccagctc
     1561 gtgatctcgg ccgtcctgag cgaggatgcc ggaccggacg agcagacgat gcacgtccag
     1621 ctggtttccg actcctcc
//
LOCUS       DAOQMF010000514         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_57464_length_1627_cov_2.701654, whole genome shotgun sequence.
ACCESSION   DAOQMF010000514 DAOQMF010000000
VERSION     DAOQMF010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57464_length_1627_cov_2.701654"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..>1627
                     /gene="rny"
                     /locus_tag="PLV77_08465"
     CDS             427..>1627
                     /gene="rny"
                     /locus_tag="PLV77_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNC15920.1"
                     /translation="MEERALRDLESARTRLREADRRSELAEERERRLDRFEQDLEEVR
                     EARLAELETVAGMTRDRARQILIREVEEEATRQAGLKVKRIEEEARLDAERRARGILA
                     VAMQRLASTHSIESNTFTIHLPNEEMKGRIIGKDGRNIRALENLTGVDIIIDETPETV
                     VISSFDGVRREVGRLTLEKLIEDGRIHPASCEAAYEEAAAKVDDVMIEAAESALLDAR
                     VTGLHPELVRLLGRLRFRTSYSQNVLSHLVECSNLAALMAAELGASVEVARRAALLHD
                     IGKAVSHEVEGTHAAVGARLARRYDEVEAITHAIEAHHNEIEPVTVEAVIVQAADALS
                     GARPGARGDSLEQYVTRLGDLEEIAGRHEGVKKVFAMQAGREIRIMVDPGQVDDEATA
                     LISHEIAA"
BASE COUNT          314 a          509 c          573 g          231 t
ORIGIN      
        1 ctgaccctgt cgtactcgcc cttcgactgg ctggaggcct acgccgcgat ccgcatcgcc
       61 ggtcgcgcgg cctgagcgga gcaccggacc ggtactgaat cttgcctgcg ggggctgtcg
      121 aaaaggggag ttgaagcgtc ggctaccctt gagggaggca tggcgttcga gtcgatcatc
      181 ccggcctggc tgcggaaccg gtctccgaaa ccggaggctg aaggcgggaa agagaccagc
      241 gggccggcaa ccaacggctc ggcgccaggg ccggcatccg gaaccggtcc gggagctgcg
      301 gctccggatc agccaacggc gacatcaggc atcacgccgg cccccgacct ggactcgcca
      361 ccgccaaggg aaagcctgct ttcggccgag tcggaactgc gggcccggcg ggaagaaatc
      421 gtccggatgg aagagcgtgc tctgcgtgat ctcgaatctg cccggacccg cctgcgcgag
      481 gcagaccggc ggtcggaact cgccgaggag cgcgagcgca ggctggaccg gttcgagcag
      541 gacctcgaag aagtccgcga agcccggctg gccgaactcg agacggtcgc cggcatgacc
      601 cgcgaccggg caaggcagat cctgatcagg gaggttgaag aagaagccac ccgccaggcc
      661 ggtctgaagg tgaaacggat cgaagaggaa gcccgtctcg atgccgagcg ccgggcccgc
      721 ggcatcctcg cggtggcgat gcagcggctc gcctccaccc actcgatcga gtcgaacacc
      781 ttcacgatcc atttgccgaa cgaggagatg aagggccgga tcatcggcaa ggacggacga
      841 aacatccggg cgctggagaa cctgaccgga gtcgacatca tcatcgacga aactccggag
      901 acggtggtga tctcgagctt cgacggggtg cgtcgcgagg ttggccggct gaccctggag
      961 aagctgatcg aggacggccg catccatccc gcttcctgtg aagccgccta cgaggaggct
     1021 gccgccaagg tcgatgacgt gatgatcgaa gcggcggaat cggccctgct ggacgcccgg
     1081 gtcaccggcc tccatccaga actcgtgcga ctgctcggtc gcctccggtt ccggaccagt
     1141 tacagccaga acgtgctcag ccacctggtc gagtgttcaa acctggccgc gctgatggcg
     1201 gccgaactgg gggcgtcggt tgaggttgcc cgtcgggcgg ccctgcttca cgacatcggc
     1261 aaggcagtga gccatgaagt cgagggaacc cacgcagcgg tgggggcgcg cctggcccgc
     1321 cggtacgacg aggtggaagc gatcactcac gcgatcgaag cccaccacaa cgaaatcgag
     1381 ccggtcacgg tcgaagccgt gatcgttcag gcggccgacg ctctctcggg tgcccgcccc
     1441 ggcgccaggg gggattccct tgagcaatac gtgacccggc tcggtgacct ggaagagatc
     1501 gccggtcgcc acgagggggt gaaaaaggtc ttcgccatgc aggcgggccg ggagatcagg
     1561 atcatggttg atcccggaca ggtcgatgac gaggcgacgg cgctgatttc gcatgaaata
     1621 gcggcgg
//
LOCUS       DAOQMF010000515         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_57630_length_1624_cov_4.762906, whole genome shotgun sequence.
ACCESSION   DAOQMF010000515 DAOQMF010000000
VERSION     DAOQMF010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57630_length_1624_cov_4.762906"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLV77_08470"
     CDS             complement(<1..411)
                     /locus_tag="PLV77_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC15921.1"
                     /translation="MATTTPESTSIQALQEQARRHLWMHFSRMGGYDDQHEIPIIARG
                     DGAYVYDAHGKRYLDGLSGLFCVNAGHGRTELAEAAARQYEELDFFVIWSYAHPRAIE
                     LATKIASMTPGDLNRVFFTSGGGEAVESALKLARN"
     gene            571..>1624
                     /locus_tag="PLV77_08475"
     CDS             571..>1624
                     /locus_tag="PLV77_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC15922.1"
                     /translation="MESPEVSSRPEPAVPNQGDQPHLKADAISFADGLAIALASTAPA
                     YSLAAVIGSIVVLVGLQAPAALLVAFVPMFFIAAAFYYMNKADQDCGTCFAWVTRAIG
                     PQAGWMTGWAICVTGILVVGSLADVAATSFFDLIGADSLMNSTAAVTGTAVALMIVMT
                     GVCVWGTEISANLQKVLMVVQIVPLILFIVVALFKVWSGDAPAGSVDPSLSWLNPFEI
                     PTGALSGAMLLAVFIYWGWESAVNLNEETEGDVDKPGLAGVISTTLLLITYVGVAFAI
                     VAWGGVSGAGKFDDNAAILATYAKQVLGGTLGLLVLLSVVVSALASTQTTILPSSRTS
                     LSMARAEAMPHALSEIS"
BASE COUNT          243 a          525 c          541 g          315 t
ORIGIN      
        1 gttgcgggcc agcttgagag cggactcgac cgcctcgccg ccgccgctgg tgaagaagac
       61 ccggttgagg tcgcccgggg tcatcgaggc gatcttcgtg gcgagctcga tcgcccgggg
      121 atgggcgtag ctccagatca cgaagaagtc gagttcctcg tactggcggg cagccgcttc
      181 ggccagttcg gtgcggccgt ggccggcgtt cacgcagaac agcccggaaa gcccgtcgag
      241 gtaccgcttg ccgtgggcgt cgtagacata ggcgccgtcg cccctggcga tgatcggaat
      301 ctcatgctgg tcgtcgtaac cgcccatgcg ggagaagtgc atccagaggt ggcggcgagc
      361 ctgttcctgc agagcctgga ttgaagttga ttcgggagtt gtggtggcca cgcgctcctc
      421 cttttccgga tggtgaagtt gggggtgtgg acaaacccta tgtcgggtaa gtcacacagg
      481 caagtcgacg gaacgtaaaa agggagcgcc gctccgttac cggacgtcgg agtttcggcc
      541 tggcgccggg tttgccacta ggtttcccgc atggagagtc cagaggtttc ttctcgtccg
      601 gagcctgctg tccccaacca gggcgaccag cctcacctca aggccgacgc gatcagtttt
      661 gccgacggtc tcgcaatcgc cctcgcctcg accgccccgg cctactccct ggccgcggtc
      721 atcggctcga tcgtcgtgct ggtcgggctg caggccccgg ccgccctgct ggtcgccttc
      781 gtgccgatgt tcttcatcgc tgccgccttc tattacatga acaaggccga ccaggactgc
      841 ggaacctgct tcgcctgggt gacccgggcg atcggacccc aggcgggctg gatgaccggc
      901 tgggcgatct gcgttaccgg catcctcgtg gtcggctcgc tggccgacgt ggcggcaacc
      961 agcttcttcg acctgatcgg ggccgacagc ctgatgaaca gcaccgcggc agtgaccggc
     1021 accgccgtcg ccctgatgat cgtgatgacc ggggtatgcg tctgggggac cgagatttcg
     1081 gccaacctcc agaaggttct gatggtcgtc cagatcgtgc cgctgatcct gttcatcgtc
     1141 gtcgccctgt tcaaggtctg gagcggcgat gccccggccg gttcggtcga cccgtcgctt
     1201 tcctggctga accccttcga gatcccgacc ggggcgcttt ccggtgcgat gctgctcgcg
     1261 gtgttcatct actggggctg ggaaagcgcg gtgaacctga acgaggaaac cgagggggac
     1321 gtcgacaagc ccggactggc cggcgtgatc tcgaccacgc tgctgctgat cacctacgtc
     1381 ggggtcgctt tcgcgatcgt cgcctggggc ggcgtgtccg gggccgggaa gttcgatgac
     1441 aacgccgcga tcctcgccac ctacgccaag caggtcctgg gcggaaccct cgggctgctg
     1501 gtcctgcttt cggtggtggt ctcggcactc gcctcgaccc agaccacgat cctgccctcc
     1561 tcccgcacct cgctttcgat ggcccgggcg gaagcgatgc cccatgcgct cagtgagatc
     1621 tcgg
//
LOCUS       DAOQMF010000516         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_57669_length_1624_cov_2.856597, whole genome shotgun sequence.
ACCESSION   DAOQMF010000516 DAOQMF010000000
VERSION     DAOQMF010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57669_length_1624_cov_2.856597"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLV77_08480"
     CDS             <1..1018
                     /locus_tag="PLV77_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15923.1"
                     /translation="LAIPLLLWIGLAALVSFGSRGLYSRPALQLLCLATVLLPVVLLL
                     TAALEPTLAIERAIATLLPVLIAALLMRIAPGWQSLALACGLTVIPYGIDMLAGSALT
                     PRAVIGPNPGLGARFYGIGNELESTLMILTSIGTGAALTRARAGGRRAAISFLVAGVV
                     ATVVFAAGRFGADVGAAIVFPVAAGVGAAFAVGRPKLAWAGLAAAFAALLLVALADIV
                     SGSETHFVRSVLGGSGGSFFEVVGHRLDATLESFTKVSRIPVTLAALAVIAIGIWKRE
                     IVLAWFEDSPMMKAGFAAAAAGSLIGALTNDSGALFIQVGTLYLALVAGFAWTTSNYH
                     PESG"
     gene            1061..>1624
                     /locus_tag="PLV77_08485"
     CDS             1061..>1624
                     /locus_tag="PLV77_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC15924.1"
                     /translation="MRIALVTPYSWTYQGGVNRHVQSLAEEYISRGHYVRVIAPFDPP
                     DRLSKVLHRAPAEDREIPDYLIPVGRTFGVNANGAVSNIPVFPSGVTQTRRAIEEGNF
                     DVVHLHEPTTPANGWDTCLSSDVPVVGTFHAYSTKPVPNYLANAVGARRTLNRLSARI
                     AVSQAAAWTGRRWYGGNYTIVPNGVDVD"
BASE COUNT          223 a          574 c          524 g          303 t
ORIGIN      
        1 gctcgcgatt ccgctgctgc tctggatcgg gctggcggcc ctggtctcgt tcggaagtcg
       61 cggcctttac tcgaggccgg cgctccagct gctctgcctc gccaccgttc tgctgccggt
      121 cgtgctgctc ctgaccgcgg cgctcgagcc gacgctggcg atcgaaaggg cgatcgcgac
      181 cctgctgccg gtcctgatcg cggccctgct gatgcgaatc gctcccggct ggcagtccct
      241 cgccctggcc tgcggcctca ccgtgatccc ctacgggatc gacatgctgg ccgggtcggc
      301 actgaccccc agggcggtga tcggccccaa tcccggactt ggtgcacgct tctacgggat
      361 cggcaacgaa ctcgaatcga ccctgatgat cctcacctcg atcggcaccg gagcggccct
      421 gaccagggcc cgggccgggg gccgtcgcgc cgcgatcagc tttctcgtgg ccggggtggt
      481 ggccacggtc gtcttcgcgg ccggccggtt cggcgccgac gtcggtgcag cgatcgtctt
      541 cccggtggcg gcgggggttg gcgccgcctt cgccgtcggc cgcccgaaac tggcatgggc
      601 gggcctcgcc gcggctttcg ccgcgctcct gctggtcgca ctggcggaca tcgtgagcgg
      661 cagcgagacc cacttcgtga ggtcggtgct gggcgggtcc ggagggtcgt ttttcgaggt
      721 ggtcggccac cggctcgacg ccaccctgga aagcttcacc aaggtctcgc ggatcccggt
      781 gaccctggcc gcgctggcgg tgatcgcgat cggtatctgg aagcgggaga tagtgctcgc
      841 ctggtttgag gattcgccga tgatgaaagc cggctttgcg gccgccgccg cgggctccct
      901 gatcggtgcg ctgaccaacg attccggcgc cctcttcatc caggtcggaa cgctttatct
      961 ggcgctggta gcgggatttg cgtggacgac gtcgaattac cacccagaat cgggttaacc
     1021 gtccctcgga aagccgccac cttcactatc ctgttctatc gtgcgaatcg ccctcgtcac
     1081 accgtattcg tggacctatc agggcggcgt taaccggcat gtccagtcgc tggcggagga
     1141 gtacatctcc cgaggccact acgtccgcgt catcgcgccc ttcgatccac ccgaccgcct
     1201 ctcgaaggtt cttcatcgtg ccccggccga agaccgcgag attcccgatt acctgatccc
     1261 ggtcggccgc accttcggcg tcaacgcgaa cggggccgtt tcgaacattc cggtcttccc
     1321 ctcgggcgtg acgcagaccc gccgggcgat cgaagaaggc aactttgacg tggtccatct
     1381 gcacgagccg accacgccgg ccaacggctg ggacacatgc ttgtccagcg acgttccggt
     1441 ggtcggcacc ttccacgcct actcgaccaa gccggtcccg aactatctgg ccaacgcggt
     1501 cggcgcccgc cgcaccctga accggctctc ggcccgaatc gccgtttcgc aggccgccgc
     1561 ctggaccggt cggcgttggt acggcggcaa ctacacgatc gtccccaacg gtgtggacgt
     1621 cgac
//
LOCUS       DAOQMF010000517         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_57811_length_1622_cov_3.093172, whole genome shotgun sequence.
ACCESSION   DAOQMF010000517 DAOQMF010000000
VERSION     DAOQMF010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57811_length_1622_cov_3.093172"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1192)
                     /gene="leuB"
                     /locus_tag="PLV77_08490"
     CDS             complement(104..1192)
                     /gene="leuB"
                     /locus_tag="PLV77_08490"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNC15925.1"
                     /translation="MSETRKIVVLAGDGIGPEIVGATRRVLDAVGSFEYEEHLIGGAS
                     IDEHGVALTDEVIEACKASDAVLLGAVGGPKWDEAVAKDPTAPRPEQGLLGIRKALGL
                     YANLRPVKPSPALIDASPLKKELIDGTDLLVVRELTGGIYFGDSGRRDDGAVAYDECA
                     YSVPEIQRIARAAFEAARQRSDSPRVLSVDKANVLETSRLWRETVKQVATEYPDVELD
                     HLLVDFAAMKLVSAPAEIDVIVTENLFGDILSDEAAMLTGSLGMLPSASIGAEGQPGL
                     FEPVHGSAPDIAGQGVANPLATMLSAAMMLRYGLGDGAGADRIDDAVDQVLAKGLRTP
                     DIFDGGEGETRVGTEEVTAAVLAELGQG"
     gene            complement(1185..>1622)
                     /locus_tag="PLV77_08495"
     CDS             complement(1185..>1622)
                     /locus_tag="PLV77_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HNC15926.1"
                     /translation="ADHDALFRSLAGATRPGGSLIAQCGGRGNVAEVIDALGVVNRQA
                     PFAEIFGEYDDPWTFAGPEETEIRLIGAGYEDVECWLEERVAHPEDPRGFFEASFLAP
                     ARQVLDDEQFTEYTDRLLAEMGQPASFNYVRLNMTAKRKSENE"
BASE COUNT          254 a          573 c          506 g          289 t
ORIGIN      
        1 tttgccgctg tgccagatgt actcagcctt ttccactgct tctccttcgc gttgcttttc
       61 ccgatgatgg tacgccagtt ccctcgggcg cactccaaga agtctagccc tgacccagct
      121 cggccagaac cgctgccgtg acttcttcgg tgccgacgcg ggtttccccc tcgccaccgt
      181 caaagatgtc cggcgtcctc aggccctttg cgaggacctg gtcgaccgcg tcgtcgatcc
      241 ggtctgcccc ggcgccgtcg ccgaggccgt agcgcagcat catcgcggcg gaaagcatcg
      301 tcgcgagcgg gtttgccacc ccctggccgg cgatgtccgg ggccgatccg tgaaccggtt
      361 caaagagccc cggctggcct tcggccccga tcgaggccga gggcagcatg cccagcgaac
      421 cggtcagcat ggccgcctcg tcgctgagga tgtcgccgaa gaggttctcg gtgacgatca
      481 cgtcgatctc ggccggagcg gaaaccagct tcatcgccgc gaaatcgact agcaggtgat
      541 cgagctcgac atcgggatat tccgtggcga cctgcttgac ggtctcccgc cagagcctcg
      601 aagtctcgag cacgttcgcc ttgtccaccg agagcacccg gggactgtcc gagcgctggc
      661 gggcagcctc gaaggcggcc cgggcgatcc gctggatctc cggcaccgag taggcgcact
      721 cgtcataggc gacggcaccg tcatcacggc ggccggaatc accgaagtag atgccgccgg
      781 tgagctcccg gacgaccagc aggtcggtgc cgtcgatcag ctccttcttc agcgggctgg
      841 catcaatcag cgccggggaa ggcttgaccg gccgcaggtt cgcgtagagg ccgagcgcct
      901 tgcgaatgcc gagcagcccc tgttccgggc gcggcgcggt cgggtccttg gccacggcct
      961 cgtcccactt cgggcccccg actgcgccga gcagcaccgc gtcggatgcc ttgcaggctt
     1021 cgatcacctc gtcggtcagg gcgaccccgt gctcgtcgat cgaggcacca ccgatcaggt
     1081 gttcctcgta ttcgaagctg ccaaccgcgt cgaggacccg acgggtcgcg ccgacgattt
     1141 ccgggccgat accgtcgcca gccagcacca caatcttccg ggtctcactc attctcgctc
     1201 ttccttttcg cggtcatgtt caggcgaaca tagttgaagc tggctggttg acccatctcg
     1261 gccagcaagc ggtctgtgta ctcggtgaac tgttcatcgt cgaggacctg ccgggccggg
     1321 gccagaaacg aagcttcgaa gaatccccgc ggatcctccg gatgggcgac ccgttcctcc
     1381 agccagcact ccacgtcctc gtaaccggca ccgatcagcc ggatctcggt ctcttccggc
     1441 ccggcgaagg tccacgggtc gtcgtactca ccgaagatct ccgcgaaggg tgcctgccgg
     1501 ttgacgaccc cgagcgcgtc gatcacctcg gccacgttgc cgcggccgcc acattgcgcg
     1561 atcaggctgc cgccgggccg ggtcgccccg gccagcgacc ggaacagggc gtcatggtca
     1621 gc
//
LOCUS       DAOQMF010000518         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58088_length_1617_cov_4.431498, whole genome shotgun sequence.
ACCESSION   DAOQMF010000518 DAOQMF010000000
VERSION     DAOQMF010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58088_length_1617_cov_4.431498"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1140)
                     /gene="pheS"
                     /locus_tag="PLV77_08500"
     CDS             complement(73..1140)
                     /gene="pheS"
                     /locus_tag="PLV77_08500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNC15927.1"
                     /translation="MVERIEEIEVEAQAAVEAADSAAELEEVRVRYLGRKAELTGMLR
                     GIADLPPEQRGPVGSTGNKVRKGLEGLIEAKVEALRNAELEQSLAGDRIDVTLPGSPA
                     RAVGHLHPITRTRRLIEDVMIGLGYQVMEGPEVEHDYYNFTALNHPEGHPARMLQDTF
                     YVDTDEHVEQVLLRTHTSPMQVRAMESAPPPLFMIVPGKTYRRDSDATHSPMFHQVEG
                     LAVGEGITLADLKGTLLAMLREIFGPDREIRMRPHFFPFTEPSVEFDVSCFRCGGSGS
                     LEDGSRCGLCKGIGWIELGGAGMVDPNVFGFVADSGYDPDSVTGFAFGFGIERIAMLQ
                     HNVGDLRLFFDNDLRMLEQFS"
     gene            complement(1169..>1617)
                     /locus_tag="PLV77_08505"
     CDS             complement(1169..>1617)
                     /locus_tag="PLV77_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC15928.1"
                     /translation="LTADKCIFLDGVADPGNVGTIIRTADALVGGTVVLGPGSADPFS
                     GIAVRSSMGSIFTQPVVKAEIGQTPAPRIATVPQGGERPGPVEGPVTLCLGSEREGLS
                     PEILKECGITWTIPQRSGGAESLNVAAAAAIACERISSQPGESAQD"
BASE COUNT          252 a          542 c          528 g          295 t
ORIGIN      
        1 ccatcttttc ggcgaggtcc cgcggttcga gcccggggtc gcagtagtcg gccatccatc
       61 cgtaagggac tctcatgaga actgctccag cattcgcagg tcgttgtcaa agaagagccg
      121 caggtcaccg acgttgtgct gcagcatcgc gattcgctca attccgaaac cgaaggcgaa
      181 tccggtcacc gagtcggggt cgtagccgga gtcggccacg aatccgaaca cgttcggatc
      241 aaccatcccg gccccgccca gttcaatcca gccgatcccc ttgcagagac cgcagcggga
      301 accgtcttcg agcgaccccg agccgccaca gcggaagcag gagacgtcga attccaccga
      361 gggttcggtg aaggggaaga agtgcgggcg catgcggatt tcccggtcgg gaccgaagat
      421 ttcgcggagc atcgccagca gggttccctt gaggtcggcc agggtgatcc cctcgccgac
      481 cgccaggccc tcgacctggt ggaacatcgg gctgtgggtg gcatcggagt cgcgccggta
      541 ggtcttgccc ggcacgatca tgaagagcgg cggcggcgcc gactccatgg cccggacctg
      601 catgggtgag gtgtgggtcc gcagcagaac ctgctcgacg tgctcgtcgg tgtcgacgta
      661 gaaggtgtcc tggagcatcc gggcaggatg gccttcggga tggttgagcg cggtgaagtt
      721 gtagtagtcg tgctcgactt ccgggccttc catcacctgg tagccgaggc cgatcatcac
      781 atcctcgatc agccgccgtg tacgggtgat cggatgcagg tggccgaccg cccgggccgg
      841 actgcccggc agggtcacgt cgatccggtc cccggccagg gactgctcca gttcggcgtt
      901 cctgagagct tcgaccttcg cttcgatcag gccctcgagg cccttccgga ccttgttgcc
      961 ggtgctgccg accgggccgc gctgctccgg tggcaggtcg gcgatacccc gcagcatgcc
     1021 ggtcagctcg gccttgcggc ccaggtaacg gacccggacc tcttcgagct cggccgccga
     1081 atctgcggct tcgacggccg cctgtgcctc gacttcgatt tcctctatcc gttcaaccat
     1141 ttcttccccg gttcagcttc ctttttcctc agtcttgtgc ggattcgcct ggttgcgaag
     1201 atatccgctc gcaggcgatc gccgccgcgg ccgcaacgtt tagcgattcg gctccgccgg
     1261 accgctgcgg gatcgtccag gtgatcccgc actccttcag gatttccggc gaaagccctt
     1321 cgcgctcgga cccaaggcag agagtcaccg gaccttcgac cggtcccggc cgctcccctc
     1381 cctgcgggac cgtggcgatc cgcggggccg gggtctgccc gatctccgcc tttacgaccg
     1441 gctgggtgaa gatcgaaccc atgctggagc gcaccgcgat cccggagaag gggtcggcgc
     1501 tgcccggccc gagcaccacc gtgccgccga ccagggcgtc ggcggtccgg atgatcgtgc
     1561 cgacgttgcc cgggtccgcg actccgtcga ggaagatgca cttgtcggcg gtcagca
//
LOCUS       DAOQMF010000519         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58108_length_1617_cov_3.119718, whole genome shotgun sequence.
ACCESSION   DAOQMF010000519 DAOQMF010000000
VERSION     DAOQMF010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58108_length_1617_cov_3.119718"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLV77_08510"
     CDS             complement(<1..889)
                     /locus_tag="PLV77_08510"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNC15929.1"
                     /translation="MSPRNYSEDELVEGPTLELLESIGYEIVDGYSEPAEFGGLGRDS
                     QTEVVLKHRLAPKLEELNPELPESAIDEAVEELVRDRATMDPVRANREVYKKLRGGVK
                     VTVVNDEGDRVEEKVRLIDWNDPANNDYLAISQFWIQGPLHKRRCDIVCFVNGIPLIL
                     MELKASHKSVHEAYDKNLRDYRDTIPKLFTQNAFVVLSTGSETRVGSSFAPWERFGEW
                     KRVDDESELGVVSLETAVDGLCSPAKLLDTVENFIAYLERPGGLIKVLAQNHQVLGVN
                     AAIRALRDRETRDGRLGVFW"
     gene            complement(886..>1617)
                     /locus_tag="PLV77_08515"
     CDS             complement(886..>1617)
                     /locus_tag="PLV77_08515"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNC15930.1"
                     /translation="EDLARSLYREWFVHFRYPGHEKSKFKDSELGAIPAGWDVQPVGE
                     VLEFIGGGTPSKRKDEFWDGGTVAWYTPSDLTSSAARYTRKSRSCITEKGLAGSSAKL
                     FPEGSVLMTSRATLGKLAIARGDSTCNQGFIVILPNACFGTQFVYEWLASEAGRLEQI
                     ATGATFKEITKGAFKKFPILVPESSVLEAFSGHADPIGEQIADFESHNEALKETRDLL
                     LPRLVTGKLDISDIDLGVLEPKEAA"
BASE COUNT          320 a          500 c          451 g          346 t
ORIGIN      
        1 gccagaacac tccgagtcgg ccgtcccggg tctcccggtc acgcagtgcc cggatcgcgg
       61 cgttcacgcc gagcacctga tggttctggg cgagcacttt gatcaacccg cctggccgtt
      121 cgaggtaggc aatgaaattc tcgaccgtat ccagcagctt ggccggcgag caaagtccgt
      181 cgaccgcagt ttcgagcgag accacaccga gctcggattc gtcgtcgacc cgtttccatt
      241 cgccgaatct ctcccagggc gcgaagctcg atccgactct tgtctctgaa ccggtcgaga
      301 gcacgacgaa ggcgttctgg gtgaacagct tcgggatggt gtcccggtag tcccggaggt
      361 tcttgtcgta ggcctcgtga accgacttgt gggaagcctt cagctccatc aggatcagcg
      421 gaattccgtt gacgaagcag acgatgtcgc aacggcgttt gtgcagtggc ccctgaatcc
      481 agaactgact gatcgccagg tagtcgttgt ttgccggatc gttccagtcg atcaggcgga
      541 ctttttcctc aacccggtca ccttcatcgt tcacgaccgt gactttgacc ccgccacgca
      601 gtttcttgta gacctcccgg ttcgcgcgca ccggatccat ggtcgcgcga tcacgaacca
      661 gttcctcgac cgcctcgtcg atagccgact ccggcagttc ggggttgagt tcctccagtt
      721 tcggagcgag ccggtgcttc agcaccacct cggtctggct gtcgcgacca agaccaccaa
      781 actcggccgg ttcggagtag ccatcaacga tctcatagcc gatcgactcc agaagctcga
      841 gagtcggccc ttcaaccagc tcatcctccg aatagttccg agggctcaag cagcctcctt
      901 cggttcgagg actccaaggt ctatatcgga aatgtccagc ttgccggtga ccaagcgggg
      961 cagcaataga tccctcgttt ccttcaaggc ctcattgtga gactcgaaat cagctatctg
     1021 ttcgccgatc gggtcggcgt gtccggagaa cgcctcaagc accgagcttt cgggaaccag
     1081 gattggaaac ttcttgaagg cccctttcgt gatttcttta aacgttgctc cagttgcgat
     1141 ctgttcaagt ctgcctgctt cggaggcgag ccattcatag acgaactgag tgccaaagca
     1201 cgcattcggg agaatcacga tgaagccctg gttgcaggtt gaatcgccgc gggcaatcgc
     1261 gagcttgccc aaggtggctc ggcttgtcat cagcacggaa ccctcgggga aaagtttcgc
     1321 tgagctgccg gcgagacctt tctcagtgat gcaagagcgc gactttcgcg tatatcgcgc
     1381 tgcggaggag gtcaaatcgc taggtgtata ccaagcgacc gttccaccgt cccagaattc
     1441 gtccttgcgc ttagaaggcg tgcctccacc aatgaactca agcacttccc cgaccggctg
     1501 tacgtcccat cccgcgggaa tcgcaccgag ctccgaatcc ttgaacttcg acttctcgtg
     1561 cccgggatac cggaagtgga cgaaccactc ccggtagaga gagcgggcaa ggtcctc
//
LOCUS       DAOQMF010000520         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58464_length_1612_cov_2.560694, whole genome shotgun sequence.
ACCESSION   DAOQMF010000520 DAOQMF010000000
VERSION     DAOQMF010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58464_length_1612_cov_2.560694"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLV77_08520"
     CDS             <1..526
                     /locus_tag="PLV77_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC15931.1"
                     /translation="CLLPWYSALDNASLALVNQGKSRREARKSAAERFEHFGLEGFET
                     ARPDELSGGMRQRVAFIRTLLSAKPVLLLDEPLAALDAITRAELQEWLTPVLRESGAT
                     VLLVTHDVEEAVYLADRVTVLSPRPGRSIATIDGMRGSPGRRDEVVSSPAFNRKREEL
                     LGLLRSDQARGAAR"
     gene            523..1281
                     /locus_tag="PLV77_08525"
     CDS             523..1281
                     /locus_tag="PLV77_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC15932.1"
                     /translation="MKARIWLPPLLLVLFLLGLWQLAASSGFLADVLGLEDFLVPAPS
                     EIADVLWQDRDLIFDNAGTTLVEIAAGFGLALISGLAVAVAFRGSDLVRRAGYPLVVA
                     SQTIPVIVIAPVLVVWFGYGIWPKLLIISLICFFPITVNTLDGLRRVDPEAVRMMRSL
                     DGSRMTIFRRVEVPTALPGVFTGARIAAAVAVIGAVFGEWAGSDKGLGHLILQDNAQL
                     MTARMFASVFVLSLIAISLYALIGLLERAVVRWR"
     gene            1340..>1612
                     /locus_tag="PLV77_08530"
     CDS             1340..>1612
                     /locus_tag="PLV77_08530"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC15933.1"
                     /translation="MRRFSLLPLAIFVALAVTACGTKSEEVNPVRDPLTVAMDWYPNP
                     DHAGFLVAEKQGYFEDAGLDVSLDSPTDPSLPIKLVAAGKADLALSY"
BASE COUNT          222 a          569 c          522 g          299 t
ORIGIN      
        1 ctgcctgctg ccctggtatt cggccctgga caacgccagc ctggccctgg tcaatcaggg
       61 gaaatcccgg cgggaggcga gaaagtcggc ggcggaacgg ttcgagcact tcggcctgga
      121 gggtttcgag accgcccgcc cggacgagct ttccggtggc atgaggcagc gcgtcgcctt
      181 catccgcacc ctcctttccg ccaaaccggt actgctgctc gacgagccgc tcgccgcgct
      241 cgacgcgatc acccgggccg agctgcagga atggctgact ccggtgctcc gggaaagcgg
      301 ggcaacggtg ctgctggtga cccacgacgt cgaggaggcg gtctacctgg ccgaccgggt
      361 caccgtcctc tccccccggc ccgggcggag catcgccacg atcgacggga tgagaggcag
      421 cccgggccgc cgcgacgagg tggtcagctc gccggccttc aaccggaagc gcgaggagct
      481 gctgggactg cttcgcagcg accaggcccg gggggcagcc cgttgaaggc aaggatctgg
      541 ctgcctccgc ttctgctggt cctcttcctg ctcggcctct ggcagctggc cgcttcgagc
      601 ggcttcctcg ccgacgtgct cggtctcgag gatttcctcg tgcccgcacc atcggagatc
      661 gccgatgtgc tctggcagga ccgcgacctg atcttcgaca acgcgggcac gaccctggtc
      721 gagatcgccg ccggcttcgg cctggccctg atctccggcc tcgcggtggc ggtcgccttt
      781 cgcggttcgg acctggtccg gcgggcgggc tacccgctgg tggtcgcctc ccagaccatc
      841 ccggttatcg tgattgcccc ggttctggtg gtctggttcg gctacgggat ctggccgaag
      901 ctgctcatca tcagtctgat ctgcttcttc ccgatcaccg tcaacacgct ggacggcctc
      961 cgccgggtcg acccggaagc ggtgcggatg atgcgctcac tggacggctc ccggatgacc
     1021 atcttccggc gggtcgaggt gccgaccgcc ctgcccgggg tattcaccgg ggcgaggatc
     1081 gccgccgcgg tcgccgtgat cggtgcggtc ttcggcgaat gggccggatc cgacaagggg
     1141 ctggggcacc tgatcctcca ggacaacgcc cagctgatga ccgcgcgcat gttcgcctcg
     1201 gtcttcgtgc tctccctgat cgcgatcagc ctttacgccc tgatcggcct gctggaacgg
     1261 gctgtcgtgc gttggcggta gctggcggca ataacctcta taggctcctt cccatcctgt
     1321 tcacgtcccc ggagatgact tgagacgatt ctcactgctc cccctcgcaa tcttcgttgc
     1381 cctcgcggtc accgcctgcg gcaccaagtc ggaggaagtg aacccggtcc gggatcccct
     1441 gaccgtggcg atggactggt accccaaccc cgatcacgcc ggcttcctgg tcgccgagaa
     1501 gcagggctac ttcgaggatg ccgggctcga cgtttcgctc gattccccga ccgatccctc
     1561 gctcccgatc aagctcgtcg ccgccggcaa ggccgacctc gcgctttcct ac
//
LOCUS       DAOQMF010000521         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58522_length_1611_cov_3.039203, whole genome shotgun sequence.
ACCESSION   DAOQMF010000521 DAOQMF010000000
VERSION     DAOQMF010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58522_length_1611_cov_3.039203"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLV77_08535"
     CDS             complement(<1..1318)
                     /locus_tag="PLV77_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC15934.1"
                     /translation="MGVLILFRNNRWRFRVLWLLTGLAAALLTAAFGHSVGLARARPN
                     LVVIQTDDQTVRMLHSHFVGPHGKKHRTMPHVQRLIEDRGLEFTNYYASIPVCSPSRS
                     ALLSGQYGHTSGLIRNVGKLGGVRGISRSPVWKRNLAVALRRVGYYTAHFGRFVNQYG
                     NPEGVDRRAVPPGWSWWATDWTPDVARRFYGYKLNVNGHIQGPFGKLHYQAWSNKDSR
                     KCPAGGLSCNYHTDQITTRALRVIRHAPRRPIYIQVDYEAPHATADGSGESEPASRHI
                     GSARRTPLPKPPGFNERNIRDKPRVLRSRARRLRAGQIAGMRTRWQRELESLRAVDES
                     VGRIVRTLRDTGRLARTYIFFIGDNGAFFGEHRYARSKFLAYEPSSNVPMLVRGPGVK
                     RGRRSGAIVSNVDLAPTIADLAGARLLLKPDGRSMKRLFRFPRRVGR"
     gene            1378..>1611
                     /locus_tag="PLV77_08540"
     CDS             1378..>1611
                     /locus_tag="PLV77_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15935.1"
                     /translation="MSDREEKAAPLDPPSILMSGLHLAALWALAIVQPLLNLLGNNPD
                     FFVARDNTSSQIIIFVLLLTILPPLFAGLIEALV"
BASE COUNT          269 a          541 c          538 g          263 t
ORIGIN      
        1 gccgtccgac ccgccggggg aaccggaaca gccgcttcat tgaccttccg tccggcttca
       61 gcagcagccg ggcgccggcg agatcggcga tggtcggcgc gagatcgacg ttgctgacga
      121 tcgcgcccga gcgacggcct cgcttgaccc cgggaccccg aaccagcatc ggcacgttgc
      181 tcgaaggctc gtaggcgagg aacttggacc gggcgtagcg gtgctcgccg aagaaggcgc
      241 cgttgtcacc gatgaagaag atgtaggtgc gggccaggcg accggtgtcc cgaagggtcc
      301 tgactatcct tccgacgctc tcgtccacgg cccggagcga ctcgagctca cgctgccagc
      361 gggtccgcat cccggcaatc tggccggccc tcaggcgccg ggcccgggaa cgcaggaccc
      421 ggggcttgtc gcgaatgttc cgctcgttga agcccggagg cttcggcagc ggcgtgcggc
      481 gggccgaacc gatgtgacgg ctggcgggct ccgactcgcc actgccgtcg gcggtcgcat
      541 gcggggcctc gtaatcgacc tggatataga tcggccgccg cggcgcgtgc cggatcaccc
      601 gcagggcccg ggtcgtgatc tgatccgtgt ggtagttgca gctcagcccg ccggccgggc
      661 atttgcggct gtccttgttg ctccaggcct ggtagtggag ctttccgaac ggcccctgga
      721 tgtgaccgtt cacgttcagc ttgtacccgt agaaccggcg ggcgacatcc ggagtccagt
      781 cggtcgccca ccaggaccat cccggcggga cggcacgccg gtcgacgccc tccgggttgc
      841 cgtactggtt cacgaaccgg ccgaaatgcg cggtgtagta accgacccgg cgcagggcga
      901 cggccaggtt ccgcttccag accgggcttc gcgaaatgcc ccttaccccg cccagcttgc
      961 cgacgttgcg gatcagcccg ctggtgtgcc cgtactgccc ggaaagcagg gccgaacggg
     1021 aaggcgaaca gaccggaatc gacgcgtagt agttcgtgaa ctcaaggccg cggtcctcga
     1081 tcaggcgctg gacatgcggc atcgtgcggt gcttcttgcc atgcggaccg acgaagtgcg
     1141 agtggagcat ccgcacggtc tggtcgtcgg tctggatgac caccaagttg ggacgggccc
     1201 gggcaagccc gaccgaatgg ccgaacgcag cggttagcag tgccgcagcc aggccggtca
     1261 gcagccagag gacgcgaaaa cgccagcggt tgttcctgaa gaggatcaag acgcccatcg
     1321 gttgaaaccc gcttcatagc ggcgagtttc ccttcaagag aataggatcg ccgaccgatg
     1381 tctgaccggg aggaaaaggc cgcacccctg gatccgccct cgatcctgat gagcggcctc
     1441 cacctcgccg ccctctgggc gctggcgatc gtccagccgt tgctgaacct gctgggcaac
     1501 aaccccgact tcttcgtcgc ccgcgacaac accagcagcc agatcatcat cttcgtcctg
     1561 ctgctgacga tcctgccgcc acttttcgcg gggctgatcg aggccctggt c
//
LOCUS       DAOQMF010000522         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58653_length_1609_cov_3.165380, whole genome shotgun sequence.
ACCESSION   DAOQMF010000522 DAOQMF010000000
VERSION     DAOQMF010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58653_length_1609_cov_3.165380"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1052)
                     /gene="lipA"
                     /locus_tag="PLV77_08545"
     CDS             complement(63..1052)
                     /gene="lipA"
                     /locus_tag="PLV77_08545"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNC15936.1"
                     /translation="MSTPPPDSATLEGSPAERIHVTRSRANPGGGTVQPDDAVPFRSR
                     KPSWLKVPPPGGPVYREIKQMIDGESLNTVCEEANCPNIGECWERGTATFMILGDVCT
                     RRCGFCNVQTGKPTHNDPLEPARVAMQVSRLGLSHAVITSVDRDDLPDYGASAFVGVI
                     RSIRAQAPDCKVEILTPDFRGQEMPLARVVHERPDVFNHNVETVPRLYPKARRGSKFL
                     RSARVLRLAREMGEGEVITKSGLMVGLGEEFDEMVETFGILREHGVQILTVGQYLRPT
                     VNHLPVVRYWHPEEFEALEKAAYEIGFESVAAGPLVRSSYHADESARKAAEAA"
     gene            complement(1059..>1609)
                     /locus_tag="PLV77_08550"
     CDS             complement(1059..>1609)
                     /locus_tag="PLV77_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15937.1"
                     /translation="GQLIAYPIVDLRGVGEQPAGADRVDVARFVASLEGAMADSLWEL
                     GIPAGRIEGLTGLWVDDERRDPGDFTDADAASAAPGLARGSIRKIGSIGLKIWRGVTS
                     HGLSLNVSCDLEPFDWINSCGIETCRATSIAVELGGEAPSVESVGKAVVASLASRLGL
                     EPTTGNPADFGLAEANPANSVA"
BASE COUNT          262 a          534 c          556 g          257 t
ORIGIN      
        1 gcaggtccag gttgtcgcga aacggaatca aggaatcagc gagggtaccg ggagaaagcg
       61 gttcaagccg cttcggcggc ttttcgtgcg ctctcgtctg cgtggtaact gctgcgaacc
      121 agcggaccgg ccgcgaccga ctcgaagccg atctcgtagg cggccttctc gagggcctcg
      181 aattcctcgg gatgccagta gcggaccacc ggcaggtgat ttacggtcgg ccgaaggtac
      241 tggccgaccg tgaggatctg gaccccgtgc tcccgcagga tgccgaaggt ctcgaccatc
      301 tcgtcgaact cctcgccgag gccgaccatc aggccggact tggtgatcac ttccccttca
      361 cccatctcgc gggccaggcg aaggaccctg gccgagcgca ggaacttcga gccgcgccgg
      421 gccttcgggt aaagccgcgg caccgtctcg acgttgtggt tgaagacatc gggacgctca
      481 tggaccaccc gggccagggg catctcctgg ccacggaagt ccggggtcag gatctcgacc
      541 ttgcagtccg gggcctgggc acggatcgaa cggatcacgc cgacgaaggc ggaagcaccg
      601 tagtccggca ggtcatcccg gtcaaccgag gtgatcaccg catgcgaaag gccgaggcga
      661 ctcacctgca tcgcgacccg ggccggttcc agcggatcgt tgtgggttgg cttgccggtc
      721 tggacgttgc agaagccgca gcggcgggtg cagacgtcgc cgaggatcat gaaggtggcg
      781 gtgccgcgtt cccagcactc gccgatgttc gggcagttcg cttcctcgca gaccgtgttc
      841 aggctctcgc catcgatcat ctgcttgatc tcgcggtaga ccgggccgcc gggcggcggt
      901 actttcagcc aggaaggctt gcgggaacgg aacggcaccg cgtcatcggg ctggacggtg
      961 ccgccgccgg gattcgcccg ggagcgggtc acgtggatcc gctcggcggg gctgccttcg
     1021 agggtcgcgc tgtcgggggg cggggtgctc accgccacct aggctacgga attggcggga
     1081 ttcgcttcgg ccaggccgaa gtcggcagga ttgccggtgg ttggctccag tccgaggcgc
     1141 gaggccagac tggccaccac ggcttttccg accgactcga ccgaaggtgc ctcgccaccg
     1201 agctcgaccg cgatcgaggt cgcccggcag gtctcgatgc cgcaggagtt gatccagtca
     1261 aacggctcga gatcgcagga aacgttcaac gaaaggccat ggctggtcac gccccgccag
     1321 atcttcagcc cgatcgagcc gatcttgcgg atgctgcccc gggccagccc cggggcggcc
     1381 gacgcggcgt cggcgtcggt gaagtccccc gggtcccggc gttcatcgtc aacccagagc
     1441 ccggtcaggc cctcaatccg gcccgccggg attcccagct cccagaggct gtcggccatc
     1501 gcgccttcga gtgaggcgac gaagcgggcc acgtcgaccc ggtcggcccc ggcgggctgc
     1561 tccccaaccc cccgcaggtc gacgatcgga taggcgatca gctggcccg
//
LOCUS       DAOQMF010000523         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58773_length_1608_cov_2.046362, whole genome shotgun sequence.
ACCESSION   DAOQMF010000523 DAOQMF010000000
VERSION     DAOQMF010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58773_length_1608_cov_2.046362"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLV77_08555"
     CDS             complement(<1..935)
                     /locus_tag="PLV77_08555"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNC15938.1"
                     /translation="MEDWLDPVYDAAGMAAADRSTIEEAGVASLDLMEAAGRGLARAA
                     AELAGGLNPGAAAGGDSLSVGGGSVTVICGKGNNGGDGLVAARHLAESGFEVEVILFG
                     DRDHLSPDSQANFDRLPEGLARVLEGPLSSSSLSGVVIDAMLGTGFDGEPRDPVASAI
                     DAINESGLAVIACDVPSGVNASTGEASGTVVRADRTVTFHQRKLGHLIAPGKHLCGPV
                     EVIPIGIAEGAPPPEAGGAIRARVLDLLPRRVADSNKFTSGRVSIVGGSRGLTGAVCL
                     AAEAAIRSGAGYATAAVPGGLEDIFEAKLTEVMDLP"
     gene            complement(935..1363)
                     /gene="acpS"
                     /locus_tag="PLV77_08560"
     CDS             complement(935..1363)
                     /gene="acpS"
                     /locus_tag="PLV77_08560"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNC15939.1"
                     /translation="MADGVGMDLIEVARIERALDRHPRLADRLFTEGELRYAASKGRP
                     GRHLAARFAAKEAVVKALRLGPGTSLREIEVVAGGEEDPAPTIRLSGRAREESGERGS
                     SVLVSLTHARELAGAVAIVGSGDSSAKEAGAERPDETGSI"
     gene            complement(1386..>1608)
                     /locus_tag="PLV77_08565"
     CDS             complement(1386..>1608)
                     /locus_tag="PLV77_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15940.1"
                     /translation="ADSDTFKAADESTAVAGLDMYANPDAIANLIVGSSPGDPDAKDA
                     ADVARKFSYMAAGSSDEDGTFEFNLGLDQ"
BASE COUNT          240 a          593 c          531 g          244 t
ORIGIN      
        1 ggaaggtcca tcacctcggt cagcttcgct tcgaagatgt cctcgaggcc gccgggaacg
       61 gctgccgtcg cgtagccggc ccccgacctg atcgccgcct cggcggccag gcagaccgcg
      121 ccggtgagcc cgcgcgagcc gccgacgatc gaaacccggc ccgaggtgaa cttgttcgag
      181 tcggccaccc ggcgtggcag caggtcgagc acccgcgccc ggatcgctcc ccccgcttcc
      241 ggtggcggcg caccctcggc aatgccgatc gggatcacct cgaccgggcc gcagagatgc
      301 ttgccggggg cgatcaggtg gccgagtttg cgctggtgga acgtgaccgt tcggtcggcg
      361 cgaaccacgg tccccgaagc ctcgccggtc gaggcgttga cgccggacgg tacatcgcag
      421 gcgatcacgg ccaggccgga ttcgttgatc gcatcgatcg cactggcgac cgggtcgcgc
      481 ggctcgccat cgaagccggt accgagcatg gcgtcgatca cgacgccgct cagcgaagaa
      541 gatgaaagcg gaccttccag gacccgggcc agaccctccg gcaaccggtc gaagttcgcc
      601 tgcgagtcgg ggctcagatg gtcccggtcg ccaaagagga tcacttcaac ttcaaagccg
      661 gattcagcga ggtgccgggc tgcaaccagt ccgtcgccgc cgttgttgcc cttgccgcag
      721 atcacggtca ccgaaccgcc tccgacgctc agggagtcac cgccggcggc ggcgcccggg
      781 ttgaggccac cggcgagttc cgccgcggcc cgggcgagac cccggcctgc cgcttccatc
      841 aggtcgagcg acgcgacgcc ggcttcctcg atcgtcgagc ggtcggccgc agccatcccg
      901 gcagcgtcat ataccgggtc cagccagtcc tccatcagat ggatcccgtc tcgtccggcc
      961 tctcggcccc ggcctccttg gcgctggaat ctccactgcc gacaattgcc acggcaccgg
     1021 ccagttccct ggcgtgagtc aggctcacca gcaccgagct gccccgttcg ccggattcct
     1081 cgcgcgcccg gcccgaaagg cggatcgtcg gcgccggatc ctcctcgccg ccagcgacca
     1141 cctcgatctc ccgcaggctc gtgcccggcc cgagccgcag ggccttgacc accgcctcct
     1201 tggcggcaaa acgggccgcc aggtggcggc ccgggcggcc cttggacgcc gcgtaccgaa
     1261 gttccccttc ggtgaacagc cggtcggcga ggcggggatg gcgatcgagg gcccgctcga
     1321 tgcgggccac ctcgatcagg tccatcccca ccccgtcggc catggctggc taaccggatc
     1381 cttccttact ggtcgagacc caggttgaac tcgaaggtgc cgtcctcgtc gctcgaaccg
     1441 gcggccatgt agctgaactt gcgagccacg tcggccgcgt ccttcgcgtc cggatcaccg
     1501 ggcgaggagc cgacgatcag attggcgatg gcgtccgggt tggcgtacat gtcgagcccg
     1561 gccaccgcgg tcgattcgtc ggccgccttg aaggtgtcgc tgtcagcc
//
LOCUS       DAOQMF010000524         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58798_length_1607_cov_4.516108, whole genome shotgun sequence.
ACCESSION   DAOQMF010000524 DAOQMF010000000
VERSION     DAOQMF010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58798_length_1607_cov_4.516108"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLV77_08570"
     CDS             complement(<1..421)
                     /locus_tag="PLV77_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNC15941.1"
                     /translation="MSEENNDFTTDQAIREESGSHPYGRFLEEFEVGATYKHMPAKTV
                     TEYDDHLFCLLTMNHHPLHINDVHANASQQGKNVVVGPLVYSLAVGMSVSDFSGKAIA
                     NLATDELSHPAPVFHGDTLFCESEVLEVRESKSKLDRG"
     gene            complement(466..831)
                     /locus_tag="PLV77_08575"
     CDS             complement(466..831)
                     /locus_tag="PLV77_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15942.1"
                     /translation="MASTAQMIESAVTHLQEEVPALKKLKLVFGLDLRAKGDMQTYRV
                     ELPGPKVSKGRGEDERCRVEIDRQQFNQLADEKSTVGSYRRAAETGHIRFEGDSNVAK
                     LVIRVIEAHEQRAKLKKVH"
     gene            complement(866..1306)
                     /locus_tag="PLV77_08580"
     CDS             complement(866..1306)
                     /locus_tag="PLV77_08580"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC15943.1"
                     /translation="MAVETIDEYLDSLPEDRREAIEAVRSAVAAGIPEGYAEELRWGM
                     ISWEIPLETYPETYNGQPLAYVSLASQKNHMALYLMGIYSDEGNRAWFENALKERGTK
                     LDIGKSCVRFKRLGDLPLDLVTEAVGRFSVNDYIQTYEKSRGLA"
     gene            complement(1334..>1607)
                     /locus_tag="PLV77_08585"
     CDS             complement(1334..>1607)
                     /locus_tag="PLV77_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC15944.1"
                     /translation="AARLVTRKAANMKDAGIRSDLEAGMAKLIASETGKECVEECFRI
                     HGGYGYSKEYEIERLYRDAPLLLIGEGTSEIQRMVIGKKLLQRHKI"
BASE COUNT          245 a          476 c          515 g          371 t
ORIGIN      
        1 ggccgcggtc gagcttgctc ttggactcgc ggacctcgag gacctcggac tcgcagaaga
       61 gcgtgtcacc gtggaaaacg ggggccgggt ggctgagttc gtcggtggcg aggttggcga
      121 tcgccttgcc ggagaagtcg gaaaccgaca tgccgactgc cagcgagtag accagcgggc
      181 cgaccacgac gttcttgccc tgctgcgagg cgttcgcatg aacgtcattg atgtgcagcg
      241 ggtggtggtt catggtcagc aggcagaaga ggtggtcgtc gtattcggtg accgtcttgg
      301 ccggcatgtg cttgtaggtg gcgccgacct cgaactcctc gagaaagcgg ccgtaggggt
      361 gcgagccgga ctcttcgcgg attgcctggt cggtggtgaa gtcgttgttt tcttcgctca
      421 ttgtctggtt ggttttcctt ggttggtgac ttaagaagtc agtgttcagt ggactttctt
      481 cagcttcgcc cgctgttcat gcgcctcgat tacgcggatg accagcttcg ccacattcga
      541 atctccctcg aaccggatgt gacctgtttc ggcggcacgc cggtaggaac cgaccgtgct
      601 tttttcgtcg gccagctgat tgaactgctg gcggtcgatc tcgacccggc agcgttcgtc
      661 ctctcctctt cccttgctca ccttcggccc gggcaactcg acccggtacg tctgcatgtc
      721 accctttgcc cggagatcga gaccgaagac cagcttcagc ttcttcaagg ccggcacctc
      781 ctcctggagg tgggtgacgg cggattcgat catctgcgct gtggatgcca tgcgtggtgt
      841 ttatcaggga aaccgcgtga ttgcctcagg cgaggccgcg gctcttctcg taagtctgga
      901 tgtagtcgtt gacgctgaac cggccgaccg cttcggtgac gaggtccagc ggcaggtccc
      961 cgagccgctt gaaccggacg caggacttgc cgatatccag cttggtcccg cgctccttga
     1021 gggcgttctc gaaccaggcc cggttgcctt cgtccgagta gatgcccatc aggtagaggg
     1081 ccatgtggtt tttctgcgag gcgagcgaca cgtaggcgag tggctggccg ttgtaggtct
     1141 ccggataggt ctcgagcggg atctcccagc tgatcattcc ccagcggagt tcttccgcgt
     1201 agccctcggg aattccggcc gctacggccg accggaccgc ttcgatcgct tcccggcggt
     1261 cttcgggaag ggaatcgaga tattcgtcaa ttgtttcgac ggccatcccg atcccttccc
     1321 tcagcccgtc agatcagatc ttgtggcgct ggagcagctt cttgccgatc accatgcgct
     1381 ggatttccga cgtgccctcg ccgatcagaa gcagcggggc gtcccggtag aggcgctcga
     1441 tctcgtattc cttcgagtag ccgtagccgc cgtggatgcg gaagcattcc tcgacgcatt
     1501 ccttgccggt ttcggaggcg atcagcttgg ccattccggc ttcgaggtcg gaacggatgc
     1561 cggcgtcctt catgttggcc gccttgcggg tgaccagccg ggctgct
//
LOCUS       DAOQMF010000525         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58892_length_1606_cov_2.352031, whole genome shotgun sequence.
ACCESSION   DAOQMF010000525 DAOQMF010000000
VERSION     DAOQMF010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58892_length_1606_cov_2.352031"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLV77_08590"
     CDS             <1..487
                     /locus_tag="PLV77_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC15945.1"
                     /translation="ALFGSMNIYDNVAFPLRQHTDKGEEEIESIVTRRLREVGLGEAH
                     FKMPNELSGGMRKRAGFARALVLDPEIVMFDEPDSGLDPVRTALLCELIKEVHAESGG
                     CYMVISHDLGTARRIADFIAVLWKGRIVESGPKDELFESDNEFVHQFLQADVVGPLAM
                     D"
     gene            489..>1606
                     /locus_tag="PLV77_08595"
     CDS             489..>1606
                     /locus_tag="PLV77_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15946.1"
                     /translation="MSRFFGGVAAATARRPWPVLILAVLLGAISIWAAGGMGSQNVTD
                     AFFDKGSSAYRQTEQADRYFGTDPVVIMAKGPLIETLDTYNLNRLATLETCLAGRIKR
                     GRGQLFRTCKEISDLHPVHTLAGPATFLGRAVAGITEVYNRQIERLSSLPDTPAGQAE
                     RSRLIALAAQVISKYGLVEAPTLEDPTFVRRVVFSEGGVRAGPKPKLNYIFPSPDAAQ
                     IVIRLRSDLTGAERTRAIGLIKEAASDPSIVLRRSQLVVSGSPVVFNGLNDELPVRVL
                     ILAAVAVLLMSLALWFTFGSIWRLLPLALALGGMAVAAGLLRLLGGEFSLASLGAAPI
                     LIGLTVDYAVQLQARFDETPPLPPEQAAREAAGLGVPM"
BASE COUNT          253 a          558 c          524 g          271 t
ORIGIN      
        1 ggcgctcttc ggctcgatga acatctacga caacgtcgcc ttcccgctcc ggcagcacac
       61 cgacaagggt gaggaagaaa tcgaatcgat cgttacccgc cggctgcgcg aggtcggtct
      121 cggtgaagct cacttcaaga tgccgaacga gctttccggc ggcatgcgca agcgcgccgg
      181 atttgcgcga gccctcgtgc tcgacccgga gatcgtcatg ttcgacgagc ccgactcggg
      241 tcttgacccg gtgcggacgg cgctgctttg cgagctgatc aaggaagtcc atgccgaatc
      301 aggcggctgc tacatggtga tcagccacga cctcggcacc gcgcgccgga tcgccgactt
      361 catcgcggtg ctctggaagg gccggatcgt cgaaagcgga ccgaaggacg aactcttcga
      421 atcggacaac gagttcgtcc accagttcct gcaggccgac gtggtcggcc cgctcgcgat
      481 ggactgagat gtcgaggttt ttcggcggtg tcgccgcggc caccgcccgt cggccctggc
      541 cggtgctgat ccttgccgta ctgctgggcg ccatttcgat ctgggcggcc ggcggaatgg
      601 gcagccagaa cgtcaccgac gccttcttcg acaaggggtc gagcgcctac cgccagaccg
      661 agcaggccga ccgctacttc ggcacggatc cggtcgtgat catggccaag gggcccctga
      721 tcgagacgct ggacacctac aacctgaatc gcctggccac cctcgagacc tgtctggccg
      781 gaaggatcaa gcggggcaga ggtcagctct tccgcacctg caaggaaatc tcggacctcc
      841 acccggtcca caccctggcc ggtcctgcca ccttcctcgg ccgcgcggtg gccggcatca
      901 ccgaggtcta caaccggcag atcgagcggc tgtccagcct gccggacacc ccggccgggc
      961 aggccgagcg cagccgcctg atcgccctgg cggcccaggt catctcgaag tacgggctgg
     1021 ttgaagcacc caccctcgaa gacccgacct tcgttcgcag ggtcgtcttc agcgagggcg
     1081 gggtccgggc cgggccgaag ccgaagctga actacatctt cccttcaccc gacgcggccc
     1141 agatcgtgat ccggctgcga agcgacctga ccggggcgga gcggacccgg gcgatcggtc
     1201 tgatcaagga agcggcaagc gacccttcga tcgtgcttcg ccgctcgcag ctcgtggtct
     1261 ccggctcgcc ggtggtcttc aacggcctca acgacgagct gccggtcagg gtgctgatcc
     1321 tggccgccgt ggcggtgctc ctgatgagcc tggcgctctg gttcaccttc ggctcgatct
     1381 ggcgccttct gccgctcgcg ctcgccctcg gtggaatggc cgtcgcggcc ggtctgctca
     1441 ggctcctcgg cggcgagttc tccctcgcct cgctgggcgc ggccccgatc ctgatcgggt
     1501 tgacggtcga ttacgcggtg cagctccagg cccgcttcga cgagacaccg ccgctgccac
     1561 cggagcaggc cgcccgcgaa gcggccggcc tcggggtgcc gatgat
//
LOCUS       DAOQMF010000526         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_58998_length_1604_cov_3.298903, whole genome shotgun sequence.
ACCESSION   DAOQMF010000526 DAOQMF010000000
VERSION     DAOQMF010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58998_length_1604_cov_3.298903"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1604)
                     /locus_tag="PLV77_08600"
     CDS             complement(<1..>1604)
                     /locus_tag="PLV77_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15947.1"
                     /translation="PRRAKTMIETGIRDSDPAVSPDGQTVLFSRDDDSSGAVDRSKLG
                     LFSIDVDGGTPTQIRAMEEIETLQLTTYTGGFAWAPDGSRIYEVEYIVKLVPAGWPYA
                     VRTRIISMLPDGSDVKVIEVGKTTFKNEKPSGDLWIASEVEPSPDGSELLVTRIPMNG
                     NAQPRIDALDLSTRKSKVIEKSAFDPSFSPDGQRIVFSTTRDKVNESCYEGSCSSSQQ
                     LYLMDADGSNPRLLLGKVQPWNFAEPDWSGDGSRILLTSNKASVTTIFGDEIWSVEPD
                     GSCLSRITNGSPASESGSWGLTPDTSPTGCGLGMGSRGVAEVEPPAKIADLEVTPLWL
                     GRNFGLLLFSDYFVDRSNASFTYWDCSWFRNRCPATIAIGSAHACERNLEGELGNGAY
                     QGMVRRKGGLAVMTGSGRPSLSTIFLADGREVWITSPWRHDGKATSRAWHMKAIDHLR
                     PYGASKPPASFGAPVFAWHTVHTARVMVRSYRRTQSLRKAAVRAGIFRAGMGISFSYS
                     RRAAQAWLRFSRDLARLGPVKSVECR"
BASE COUNT          293 a          522 c          485 g          304 t
ORIGIN      
        1 gtctgcattc gaccgacttg acgggtccca gccgggccag atcacggctg aacctgagcc
       61 aggcctgcgc cgctcgcctc gagtagctga agctgatgcc catcccggcc cggaagatcc
      121 ccgcccgaac ggccgccttc ctcagggatt gagtccgtcg gtagctgcgc accatcaccc
      181 gcgcggtgtg cacggtgtgc caggcgaaga ccggggcccc gaagctggcc ggcggcttgc
      241 tcgccccgta cggacgcaga tggtcgatcg ccttcatgtg ccaggcccgc gaagtcgctt
      301 tgccgtcatg tcgccaaggc gaggtaatcc agacctcacg accatcggcg agaaagatgg
      361 ttgaaagcga cggcctgccg ctgccggtca tcactgccag tccacccttg cgacggacca
      421 ttccctgata ggccccgttt ccgagctctc cctcgaggtt gcgctcgcag gcatgggccg
      481 atccgattgc gatcgtggcc gggcaacggt tcctgaacca ggaacagtcc cagtaagtga
      541 agcttgcgtt gcttcgatcg acgaaatagt cgctgaacaa cagcagcccg aagttcctgc
      601 ccagccacag aggcgtcacc tcgaggtctg cgattttcgc gggtggttcg acctccgcaa
      661 ctcctctcga acccatcccg aggccgcaac cggtcgggga ggtatccggg gtcaatcccc
      721 aggaaccgga ttcactggcc gggctgccgt tggtgattcg gctcaggcaa gagccatccg
      781 gctccaccga ccagatctca tcgccgaaga ttgtcgtgac cgaagccttg ttgctggtca
      841 ggagtattcg cgagccgtct ccggaccagt cgggctcggc gaaattccag ggctgaacct
      901 tcccgagcag caaccgcggg ttcgaaccgt ccgcgtccat cagatacagc tgctgggacg
      961 acgagcagct gccttcgtaa caactttcgt tcaccttgtc gcgggtcgtc gaaaagacga
     1021 tcctctggcc gtccggtgaa aatgagggat cgaaggccga cttctcgatc accttcgatt
     1081 tccgggtaga aaggtccaac gcgtcgattc ggggctgcgc gtttccgttc atcgggatgc
     1141 gggttaccag gagctccgac ccgtccggtg aaggttcgac ttcagacgcg atccagaggt
     1201 ctccggatgg cttctcgttc ttgaaggtcg tcttgccgac ttcgatgacc ttgacgtcgc
     1261 tgccgtcggg aagcatcgag atgatccggg tccggaccgc atacggccag ccggccggaa
     1321 ccagcttgac gatgtactcg acctcataga tccgcgaacc atccggtgcc catgcgaagc
     1381 cgccggtgta ggtggtcagc tggagggtct cgatctcctc cattgccctg atctgggtcg
     1441 gagttccgcc gtcgacgtca atcgagaaaa ggccgagctt cgaccggtca accgccccgg
     1501 atgaatcgtc gtcccgcgaa aagagcacgg tctgcccgtc cggagagaca gccggatccg
     1561 aatcccggat cccggtctcg atcatcgtct ttgcccgccg cggg
//
LOCUS       DAOQMF010000527         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_59126_length_1602_cov_4.061409, whole genome shotgun sequence.
ACCESSION   DAOQMF010000527 DAOQMF010000000
VERSION     DAOQMF010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59126_length_1602_cov_4.061409"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLV77_08605"
     CDS             complement(<1..402)
                     /locus_tag="PLV77_08605"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC15948.1"
                     /translation="MASYSTSVESPKPVEEAFDYMADFRNAEEWDENTSSVKLTSGQA
                     GQAGSEYEVITGFAGRDMKLSYRTVEVDRPSKVVFESSTGMAKIRDTIEFSGDEKGCE
                     VKYDARILTNGLAKVIDPLFSLIFKRVGDRAA"
     gene            complement(461..988)
                     /locus_tag="PLV77_08610"
     CDS             complement(461..988)
                     /locus_tag="PLV77_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15949.1"
                     /translation="MAPGFQSLLSGGDPRSLGRVDEVITTVLDDPGSCRELLDCLYGE
                     DEIVRMRAADGLEKVAATQPGIVEPFLPELLGPVARIDQPSVKWHLAQIFAEVELGEA
                     DRRRATALVEGYLEDSRDWIVITCSMTTLTGFAASDAELRERLVPVLRLHLDSDRKAI
                     AKRASKMLAELGETS"
     gene            complement(1003..>1602)
                     /locus_tag="PLV77_08615"
     CDS             complement(1003..>1602)
                     /locus_tag="PLV77_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNC15950.1"
                     /translation="DRDEVRPATGGGPDFITIDGGEGGTGAAPLAFADHVSLPFKLGF
                     ASAYSSLARAGLAGDIVFIGAGRLGFPDAALFASALGCDMFNVGREAMMSIGCIQAQR
                     CHTGGCPAGVATQSRWLMGGLDPQLKSTQAANYIVNLRAELLALARSCGVSHPAQVTP
                     DHIEIVSERYGHAPLREVFGYEADWPIPGTVAPELAADA"
BASE COUNT          264 a          534 c          527 g          277 t
ORIGIN      
        1 ggcagcccgg tcgccgaccc gcttgaagat cagcgagaag agggggtcaa tcaccttggc
       61 caagccgttg gtcaggatgc gggcgtcgta cttcacctcg cagccttttt cgtctccact
      121 gaactcgatc gtgtcgcgaa tcttcgccat gccggtcgaa gattcgaaga cgaccttcga
      181 gggacgatcc acctccaccg tccggtagga gagcttcatg tcccggccgg cgaagccggt
      241 gatcacctca tattcggaac cggcctggcc ggcctggccg gaggtcagtt tcaccgatga
      301 ggtgttttcg tcccattcct ccgcattgcg gaaatccgcc atgtagtcga acgcttcttc
      361 gaccggcttc gggctttcta cggatgttga gtagctggcc acggggaaag tctttcgcat
      421 ccgcctcacc ggcgtggaag cggagcccgg ccgctgagcc tcaactggtt tcgccaagct
      481 ccgccagcat ctttgaggcc cgtttggcga tcgccttgcg gtccgagtcc aggtgcaggc
      541 gcaagacggg caccagccgc tcacggagtt ccgcgtcact ggccgcgaaa ccggtcaggg
      601 tcgtcatcga gcaggtgatc acgatccagt cgcgggaatc ttcgaggtag ccctcgacga
      661 gcgccgtcgc ccgccgccgg tccgcctctc cgagttccac ttcagcgaag atctgggcca
      721 ggtgccactt gactgatggc tggtcgatcc gggccaccgg cccgagcagc tccggcagga
      781 agggttcgac gatcccgggc tgtgtggccg ccaccttctc gagcccgtcg gcggctcgca
      841 ttcgcacgat ctcgtcttcg ccgtaaaggc aatcgagaag ttcgcggcag gaaccgggat
      901 catcgaggac cgtcgtgatc acctcgtcga cccggcccag agaccgggga tctcccccgc
      961 tcagaagcga ctggaatcca ggcgccattc cggcctgccc gcctatgcgt cggcggccag
     1021 ttcgggggcg acggtgcccg gaatcggcca gtcggcttca tatccgaaca cttcgcgcag
     1081 cggagcgtgg ccgtagcgct cggagacgat ctcgatgtgg tccggggtga cctgcgccgg
     1141 gtgggaaacg ccgcagctgc gggcaagggc cagcagttcg gcccgcaggt tgactatgta
     1201 gttggcggcc tgggtcgatt tcagttgcgg gtccagcccg cccatcagcc agcgcgactg
     1261 ggtggccacc ccggccgggc agcctcccgt gtggcagcgc tgcgcctgga tgcagccgat
     1321 cgacatcatc gcttcccggc cgacgttgaa catgtcgcag ccgagcgcgg aagcgaacag
     1381 cgcggcgtcg gggaaaccta gccgccccgc accgatgaag acgatgtccc cggccaggcc
     1441 ggcccgggca aggctcgagt aggcgctcgc gaagccgagt ttgaagggca gcgagacatg
     1501 gtcggcgaat gccagcggcg ccgcgccggt gccgccctcg ccgccgtcga tcgtgatgaa
     1561 gtccggcccg ccaccggtgg cgggccggac ttcatcacga tc
//
LOCUS       DAOQMF010000528         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_59303_length_1600_cov_2.611650, whole genome shotgun sequence.
ACCESSION   DAOQMF010000528 DAOQMF010000000
VERSION     DAOQMF010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59303_length_1600_cov_2.611650"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLV77_08620"
     CDS             <1..360
                     /locus_tag="PLV77_08620"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15951.1"
                     /translation="HDFISFYDQEIEFRQQLKYPPLSRIALLTLKGRNEEKVRFTAEH
                     VRKELDRALAGWSDLVLAGPAPAPLARAESFYRYQVMLRTRHMSRASQVIAALVRDWS
                     LPEETSLTVDIDPVNLS"
     gene            complement(385..>1600)
                     /locus_tag="PLV77_08625"
     CDS             complement(385..>1600)
                     /locus_tag="PLV77_08625"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNC15952.1"
                     /translation="LNRTRAQTGSNPSEAIRVGIIGTGGQGGSNMTTPTMVKHVTAVC
                     DVDRNRVAEAQKQLKEKTGIDAVGYGDYRKLLESKEIDAVLIATPDHWHALTAIHACQ
                     AGKDVYVEKPMTLTIIEALAMEKAARHYNRIVQTGSQQRSWPDQKFRRACEYVRSGRI
                     GDVKMVRVGLPGVNWTKDPAVPDSAPPPELDYDLWLGPAPQRPYNKHRVHYFFRFFWD
                     YSGGQMTNWGAHHLDITQWALNMDESGPVEVTARAEYDPQKRYEVPTWFEITYTYANG
                     VTVICGQSHRIGTTFEGQDGIIYVDRGKLESTVKDALNEPLGNDEVHLTVSRDHHQNW
                     FDSIKSRKPPICDVAIGKRSATVCHLGNIAVQTGQPVRWDPVKEQIVGDAPKVKQLTY
                     EYRKPWELPKIG"
BASE COUNT          245 a          526 c          501 g          328 t
ORIGIN      
        1 cacgacttca tcagcttcta cgatcaggaa atcgagttcc gccagcagct gaagtacccg
       61 ccgctttcgc ggatcgctct gctcacgctg aagggacgga acgaggagaa ggtccggttc
      121 accgccgagc acgtccgtaa ggagctggat cgcgccctgg ccggctggtc ggatctggtg
      181 ttggccgggc cggcgccggc gccactcgcc cgggcggaga gcttttaccg ctatcaggtg
      241 atgctccgga cccggcacat gtctcgagcc agccaggtca tcgcggcact ggtccgcgac
      301 tggtcgctgc cggaggaaac cagcctcacg gtcgacatcg atccggtcaa cctctcctga
      361 gaccggccgg gcgccgatgc gcgctcaacc gatcttgggc agttcccagg gcttgcgata
      421 ctcgtaggtc agctgtttca ccttcggcgc gtcgccgacg atctgttcct tgaccgggtc
      481 ccaccggacc ggttggccgg tttggacggc gatgttgccg aggtggcaca ccgtcgccga
      541 gcgtttgccg atcgccacgt cgcaaatcgg cggtttgcgg ctcttgatcg agtcgaacca
      601 gttctggtga tggtcgcgac tgacggtcag gtggacctcg tcgttgccca gcggttcgtt
      661 caaggcgtcc ttcacggtcg attcgagctt gccgcggtcc acgtagatga tgccgtcctg
      721 cccttcgaag gtggtgccga tccggtggct ttggccgcaa atcaccgtca cgccgttcgc
      781 gtacgtatag gtgatttcga accacgtggg gacttcgtaa cgcttctggg ggtcgtactc
      841 ggcccgggcc gtgacctcca ccggcccgct ctcatccatg ttcaacgccc actgggtgat
      901 gtcgaggtga tgcgcgcccc agttggtcat ctgaccgccg gagtagtccc agaagaaccg
      961 gaagaaataa tgcacccgat gcttgttgta cggacgctgg ggtgcggggc cgagccacag
     1021 atcataatcg agttccggcg gaggggcgct gtcgggaacg gccgggtcct tggtccagtt
     1081 cacccccggc aggccgaccc gcaccatctt cacatccccg atgcggccgg agcgcacgta
     1141 ttcgcaagcc cgacggaact tttgatccgg ccacgaccgc tgctggctgc cggtctggac
     1201 gatccggttg taatggcggg ccgccttctc catcgccaac gcctcgatga tcgtcagcgt
     1261 catcggcttc tccacgtaaa cgtccttgcc cgcctgacag gcatggatcg ccgtcagcgc
     1321 gtgccagtgg tccggcgtgg cgatcaggac ggcgtcgatt tccttgcttt caagcagctt
     1381 gcggtaatcc ccgtatccca ccgcgtcgat gcccgttttc tccttcagct gcttttgcgc
     1441 ctcggccacg cgattgcggt cgacgtcgca caccgcggtg acgtgcttca ccatcgtcgg
     1501 cgtcgtcatg ttgctgcccc cctgcccgcc ggtgccgatg atgcccacgc gaatcgcctc
     1561 gctgggattc gacccggtct gggcgcgggt gcgattcaga
//
LOCUS       DAOQMF010000529         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_59426_length_1598_cov_3.220350, whole genome shotgun sequence.
ACCESSION   DAOQMF010000529 DAOQMF010000000
VERSION     DAOQMF010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59426_length_1598_cov_3.220350"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLV77_08630"
     CDS             <1..693
                     /locus_tag="PLV77_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC15953.1"
                     /translation="ATAFAWLADQAPLRGLIISGAGKAFCAGADLNWMKKGQEDPDTD
                     IVASVRRGAELLHSGIVDLQRIPYPVIAALNGPTAGAGFSLALACDIRICSEDAFLAC
                     AYGRIGVSPDGGMTYFLPRVVGPSKAIEILLNDPNLSPADALEAGLVSEVVAPDQLLD
                     RAREKADKLAKKSPYYVKMAKHLVHQSLDNSITEQLQLERHGIANGMGTEDSKNAIEA
                     FLTGGTAEFKGE"
     gene            complement(731..1216)
                     /locus_tag="PLV77_08635"
     CDS             complement(731..1216)
                     /locus_tag="PLV77_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNC15954.1"
                     /translation="METLAIEGPEQVQELVGKTVGPTGWREVTQQMIDTFADLSGDHQ
                     WIHVEVERAKNESPFGTTIAHGNLTLSLVDGFRLDLIEVKSGFALGVNYGWNKVRFPA
                     PVPAGSKVRASAELTEANDLGNGWLELITRFTLEVQAPDGSIGEKPCFVGESVTRALK
                     A"
     gene            complement(1267..>1598)
                     /locus_tag="PLV77_08640"
     CDS             complement(1267..>1598)
                     /locus_tag="PLV77_08640"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNC15955.1"
                     /translation="SGFDPNAGNGIAFGIAAFTPAAAAEYGLDDPFDPAAAIMAQAHL
                     MSDLLRQFGSPALALAAYNAGPGAVGSCDCIPSYPETQAYVAKILGMLGGMGAISPIG
                     MTIELVR"
BASE COUNT          270 a          541 c          514 g          273 t
ORIGIN      
        1 gccacggctt tcgcctggct tgccgaccag gccccgctgc gcggactgat catctccgga
       61 gccggcaagg ccttctgcgc cggcgccgac ctcaactgga tgaagaaggg ccaggaagac
      121 ccggacaccg acatcgtcgc ctcggtccgg cgcggggccg agctgcttca ttccggcatc
      181 gtcgacctgc agcggatccc ctacccggtg atcgccgccc tcaacggccc gaccgccggg
      241 gccggcttct cgcttgccct ggcctgcgac atccggatct gttccgagga cgcctttctc
      301 gcctgtgctt acggccggat cggggtttcg ccggacggcg gcatgaccta tttcctgccc
      361 cgggtggtcg gcccctcgaa ggcgatcgag atcctgctca atgacccgaa tctctcccct
      421 gccgacgcgc tcgaggccgg tctcgtctca gaggtcgtgg caccggacca gttgctcgac
      481 cgggcccgcg aaaaggccga caagctggcg aagaagtccc cctactacgt gaagatggcc
      541 aagcacctgg tccatcagag tctcgacaac agcatcaccg agcaactcca gctggagcgt
      601 cacggcatcg ccaacggcat gggcaccgaa gactcgaaga acgcgatcga ggccttcctc
      661 accggcggaa ccgccgaatt caaaggcgag tagccgccgg ccggggccgg gcatcgggct
      721 ttttggcagg tcaggctttg agggcgcggg tcacgctctc gccgacgaag cagggcttct
      781 cgccgatgct tccatcgggg gcctggacct cgagggtgaa cctggtgatc agttccagcc
      841 agccgttgcc gaggtcgttc gcctcggtca gctcggccga ggcgcggacc ttcgagccgg
      901 ccgggaccgg cgccggaaag cggaccttgt tccagccgta gttcacgccg agggcgaacc
      961 ccgacttgac ctcgatcagg tcgagccgga aaccgtcgac cagggagagc gtgaggttgc
     1021 cgtgcgcgat cgtggtgcca aaggggcttt cgttctttgc cctctcgacc tcgacgtgga
     1081 tccactgatg gtcaccggaa agatcggcga aggtgtcgat catctgctgg gtgacttcgc
     1141 gccagccggt cgggccgacc gtcttgccaa ccagttcctg cacctgctcg gggccttcaa
     1201 tcgcgagagt ttccatggcg caacattatc ggcacctgtt gcccctcggg caacaattcc
     1261 ctgccgctac ctgaccagtt cgatggtcat gccgatcgga ctgatcgctc ccatgccacc
     1321 gagcatgccg agaatcttcg ctacataggc ctgggtttcg ggataggacg ggatgcagtc
     1381 gcaggaaccg accgcgcccg gaccggcgtt gtatgcggcc agggcgagag cgggcgaacc
     1441 gaactgccgg agcaggtcgc tcatcaggtg ggcctgggcc atgatcgccg ccgccgggtc
     1501 aaacggatca tccagcccat actcggccgc cgcggccggc gtgaaggccg cgatgccgaa
     1561 ggcgatcccg ttgccggcgt tcgggtcaaa gcccgatt
//
LOCUS       DAOQMF010000530         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_59758_length_1593_cov_4.362159, whole genome shotgun sequence.
ACCESSION   DAOQMF010000530 DAOQMF010000000
VERSION     DAOQMF010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59758_length_1593_cov_4.362159"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="PLV77_08645"
     CDS             complement(<1..1568)
                     /locus_tag="PLV77_08645"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNC15956.1"
                     /translation="MKVAGKFAVALSVLLGVFVFTGSASATTFCVPGYFDGCPDNGSN
                     VVPSGAGAGEKLENALSQNGSDGSPDTVYLDSGTYVDTDSLKAGGSDGLTVIGNGRDR
                     SFITSSVAGNYYVVDLNFGNSRHITMKDLAIVIPEDFPNDNGPGSAVQSKGDDFDSVD
                     VINENNTGTVYLSSGFANVMGGGSFTDIRLFGRNGARFGSAINVACNYPGQVTINGAG
                     VSDSQYGVLAPCSTVPVSIDRATFSGADSALQPSNGAQLTASNVLIESGDLSPVMVYN
                     AKGAGTTTLDLDHATVVATGDASMPAIRANVANVVAATDPIQVNVSNSIILGFDDTWE
                     MIAPTDPAKGDVGLSVSYSDFSPVGVVTGDSSLDTATGNIFDSPGFTGITDYHLTPGA
                     PTVDAGDPSAAGPLFDLEGNGRPIDGDFDSVAVPDMGAYELNPPPPNCPVDPSACAPK
                     LRKVKFRYRFGKGGTLRYRVSIKAKVIAVFRPVPKKAKSKRMTVRILKQGKQGANVIK
                     LGKRLLKPGRYRLA"
BASE COUNT          274 a          489 c          534 g          296 t
ORIGIN      
        1 atcgcgagcc ggtagcggcc cggcttcagc aggcgtttgc cgagcttgat cacgttggcg
       61 ccctgctttc cctgcttgag gatgcggacc gtcatacgct tcgactttgc cttcttcggg
      121 accggcctga agacggcgat caccttcgcc ttgatcgaga cccggtaccg cagggtcccg
      181 cccttgccga accggtagcg gaacttgacc ttccggagtt tcggggcgca ggcggacggg
      241 tcgaccgggc agttcggggg aggcgggttg agttcgtagg cgcccatgtc cgggaccgcg
      301 acgctgtcaa agtcaccgtc gatcgggcgg ccgttccctt cgaggtcgaa gagcggcccg
      361 gcggcggaag gatccccggc gtcaacggtc ggcgcgccgg gggtcaggtg gtagtcggtg
      421 atcccggtga acccggggct gtcgaagatg ttgccggtcg cggtgtcaag gcttgaatcg
      481 ccggtcacca cgccgaccgg ggaaaagtcg gagtaactga ccgaaaggcc gacgtcgcct
      541 ttggccggat cggtcggggc gatcatctcc caggtgtcgt cgaagccgag aattatcgag
      601 ttgctcacgt tgacctgaat cgggtccgtg gcggcaacga cattggccac gttggcccgg
      661 atcgccggca tcgacgcatc ccccgttgcg accacggttg cgtgatcgag gtcgagcgta
      721 gtcgtgccgg cgcccttggc gttgtagacc atgaccgggg agaggtcgcc actttcgatc
      781 agcacgttcg aggcggtgag ctgcgcaccg ttggacggct gcagcgccga atccgccccg
      841 gagaaggtgg cccggtcaat cgaaacgggg accgtggagc agggagccag gacgccgtac
      901 tggctgtcgg agaccccggc gccgttgatc gtgacctgcc ccggatagtt gcaggcgaca
      961 ttgatcgcgc tgccgaaccg ggcgccgttt ctcccgaata gccggatgtc ggtgaagctg
     1021 cccccgccca tcacgttcgc aaatccggac gagagataaa ccgttccggt gttgttctcg
     1081 ttgatcacgt caactgaatc gaagtcgtca cccttggact ggaccgccga gccgggcccg
     1141 ttgtcattcg ggaagtcctc ggggatcacg atcgccagat ccttcatcgt gatgtgacgg
     1201 gagttgccga agttgaggtc aaccacgtag taattgccgg cgaccgaact ggtgatgaag
     1261 ctcctgtcgc ggccgttgcc gatcacggtc aaaccgtcgc tgccgccagc cttcagggaa
     1321 tcggtgtcga cgtaggtccc gctgtccagg tagaccgtgt ccggactgcc gtcgctgccg
     1381 ttctgggaca acgcattctc gagcttttca ccggccccgg cgccggaggg gaccacgttc
     1441 gagccgttgt cggggcagcc gtcgaagtag ccgggcacgc agaaggtggt tgccgatgcc
     1501 gaaccggtga agacgaaaac cccgagcagg accgacagcg caactgcgaa cttccccgca
     1561 accttcattc agaactcccc ttttcttgac agg
//
LOCUS       DAOQMF010000531         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_59855_length_1592_cov_3.013663, whole genome shotgun sequence.
ACCESSION   DAOQMF010000531 DAOQMF010000000
VERSION     DAOQMF010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59855_length_1592_cov_3.013663"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLV77_08650"
     CDS             complement(<1..486)
                     /locus_tag="PLV77_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15957.1"
                     /translation="MTLALFAATLVSLLAASPSVGALVPRFALDGSPGPSGSYPFDLT
                     DLNGTLLFSAEIERGNELYATSDGRKIRLVRDLLSGPDDGDPSDLTRFGNFVYFLGYE
                     KNAGYEMWRTNGRSASLFAEPSPGPDSSNIDFLTVADGFLYFSAETPAAGRELWKTDG
                     QA"
     gene            498..>1592
                     /locus_tag="PLV77_08655"
     CDS             498..>1592
                     /locus_tag="PLV77_08655"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNC15958.1"
                     /translation="MCFQLRASFPRSSASAPTLAPARRAVKRPRSNRYASVMEVTAAD
                     IAADTVRAKSAARAMAAAPTGVKNRALEAIARGLGESVDGILAANASDLADERAQGLS
                     RALTDRLTLTPERVGAMAAGVREVAALDDPVGEVLESRTLENGLELSKIRVPLGVVAV
                     VYEARPNVTIDCAALTIKSGNSILLKGSSYAENTNLALAEVVRSGLAEAGLPEDGVVM
                     SVGGGRESIADLATQDGVVDLLIPRGGEGLKKTLEEVATVPVIFAAAGICHVYVHSDA
                     NLEMARAIAYNAKIQRPGVCNAAETLLVDSAVAAAFLPPALAELSGGGVELVGDESTR
                     ENAGDTPVGEATEADWDTEYLDLKLAVKVVD"
BASE COUNT          293 a          508 c          542 g          249 t
ORIGIN      
        1 cgcctggccg tcggtcttcc agagctccct tccggcagcc ggggtttcgg cggagaagta
       61 gaggaaaccg tcggccacgg tcaggaagtc gatgttcgat gagtccggtc cgggggaagg
      121 ctcggcgaac agcgaagcac tcctgccgtt ggttctccac atttcgtatc cggcgttctt
      181 ctcgtagccg aggaagtaga cgaagttgcc gaatctggtg aggtcggaag gatccccgtc
      241 gtccggtccg gaaagcaaat cccggaccag cctgatcttc ctgccatcac tggttgcgta
      301 caactcattg ccgcgctcga tctcggccga aaacagcagg gtgccgttga ggtcggtcag
      361 gtcgaacgga taagacccgc tgggcccggg actgccatca agggcaaagc ggggaacaag
      421 ggcgcccacc gagggggagg ccgccagcag cgacaccaaa gtggcggcga acaaggccag
      481 cgtcaaccgg accttcaatc tgtttccaac tccgtgcaag cttcccccgt agcagtgcgt
      541 ctgccccgac gctagcgccc gcacggcgcg cggtcaagcg gcctcgatcg aacaggtacg
      601 cttcagttat ggaagtgacc gccgccgaca tagccgccga tactgtcaga gccaaaagcg
      661 ccgcccgcgc catggccgcg gctccgaccg gggtcaagaa ccgggcgctc gaggcgatcg
      721 cccgtggtct cggtgaatcg gttgacggga ttctcgccgc gaacgcttca gatctggccg
      781 acgaaagggc ccaggggctt tccagggcgc tgactgaccg gcttacgctc acgcccgagc
      841 gcgtgggagc gatggccgcc ggggtgcggg aggtcgctgc cctcgacgac ccggtcgggg
      901 aagtgctgga atcgcgcacc ctcgagaacg ggcttgagct gagcaagatc agggtgccgc
      961 tcggcgtcgt cgccgtggtt tacgaggcgc ggccgaacgt gaccatcgac tgcgctgccc
     1021 tgaccatcaa gtcaggcaac tcgattctgc tcaaggggtc cagctacgcc gagaacacga
     1081 acctggcgct ggccgaagtc gtgcgatcag gcctggccga agccggcctg cccgaggacg
     1141 gcgtcgtgat gtcggtcgga ggcggccgcg agtcgattgc cgacctcgcc acccaggatg
     1201 gcgtggtcga cctgctgatt ccccgcggcg gtgaagggct gaagaagacg ctcgaagagg
     1261 tggcgaccgt accggtgatc ttcgctgcgg ccgggatctg tcatgtctac gtccactccg
     1321 acgcgaacct ggagatggcc cgggcgatcg cctataacgc aaagatccag cgccccggcg
     1381 tctgcaacgc ggccgagacc ctgctggtgg acagcgcagt cgccgctgcc ttcctgccgc
     1441 ccgccctggc cgagctttcc ggcggcggag tcgagctggt cggggacgaa tcaacccgcg
     1501 agaacgccgg ggacacaccg gtcggcgaag cgaccgaggc cgattgggac accgagtacc
     1561 tcgacctgaa actggccgtg aaggtggtgg ac
//
LOCUS       DAOQMF010000532         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_59939_length_1591_cov_2.790365, whole genome shotgun sequence.
ACCESSION   DAOQMF010000532 DAOQMF010000000
VERSION     DAOQMF010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59939_length_1591_cov_2.790365"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /gene="hflX"
                     /locus_tag="PLV77_08660"
     CDS             <1..985
                     /gene="hflX"
                     /locus_tag="PLV77_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNC15959.1"
                     /translation="PVIDRTAIILDIFADHANSAEGKLQVELAQLQYNLARMRGLWTH
                     LERLGAGIGTRGPGESQIETDRRLARDRISDLRRKLRHVEQNRGVMRSRRQDSSLPQV
                     ALAGYTNAGKSTLLNALTGAGIGVADRLFHTLDPTTRAFEYEGRRYLVTDTVGFIKKL
                     PHQVVEAFKATLEETVLADLILHVVDASAGEDRMQEEMAAADAVLDEIGAGEKPRLLV
                     LNKIDLLDDDQRAEARIDHPDAVQVSAVSGEGLDLLRARMERAFEETMAPVELLIPYA
                     EGARLHELHEVAGKVDREDRPEGVLVRTRLPLGEMHRFADLATPREAESII"
     gene            997..1428
                     /gene="dut"
                     /locus_tag="PLV77_08665"
     CDS             997..1428
                     /gene="dut"
                     /locus_tag="PLV77_08665"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNC15960.1"
                     /translation="MELKVQLLNDSATLPTRAHEGDAGLDLYASEPAHIGPGERWQVA
                     TGIAVEIPEGHAGMVLPRSGLARRHGISLVNAPGLVDAGYRGEVKVLLLNNDPAEVFR
                     INPGDRIAQMVVIPLAAVEPVATDSLSETERGEGGFGSSGR"
BASE COUNT          273 a          517 c          554 g          247 t
ORIGIN      
        1 gccggtgatc gaccgtaccg cgatcatcct cgacatcttc gccgaccacg ccaattcggc
       61 cgaaggcaag ctgcaggtgg agctggcgca gctgcagtac aacctggccc ggatgcgggg
      121 cctctggacc cacctcgagc ggctcggcgc gggaatcggc accagggggc cgggcgaatc
      181 ccagatcgag accgaccgcc ggctggcccg cgaccggatc tccgacctgc gccggaagct
      241 ccggcacgtc gaacagaatc gcggtgtgat gcgaagtcgc cggcaggatt cctcgctgcc
      301 gcaggtcgca ttggccggct acaccaacgc cggcaagtca accctgctga acgccctgac
      361 cggagccggt atcggggtgg ccgacaggct gttccacacg ctcgacccga ccacgcgcgc
      421 cttcgagtac gaaggccggc ggtacctggt caccgacacg gtggggttca tcaagaagct
      481 gccccatcag gtggtcgaag ccttcaaggc aaccctcgag gaaaccgtgc tggccgacct
      541 gatcctgcat gtggtcgacg cctcggcggg cgaggaccgg atgcaggagg aaatggctgc
      601 cgccgacgcc gtgctcgatg agatcggggc cggtgaaaag ccccgtctgc tggtcctgaa
      661 caaaatcgac ctgctggacg atgaccagcg ggcggaggcg cggatcgacc atcccgatgc
      721 ggtccaggtt tcggcggtca gcggggaagg cctcgacctg ctgcgggccc ggatggagcg
      781 tgccttcgaa gagacgatgg ctccggtcga gctgttgatc ccatacgccg aaggcgctcg
      841 tcttcatgag cttcacgaag tggccggcaa ggtcgaccgc gaggaccggc cggaaggggt
      901 gctggtcaga acccgactgc cgctcggcga gatgcaccgc ttcgccgacc tcgcgacacc
      961 aagagaagcc gaatcaatca tctagagtcc ccggcgatgg aactcaaggt tcagttgcta
     1021 aacgattccg cgaccctgcc gacccgcgcc cacgaagggg acgccgggct ggacctctac
     1081 gcctcggagc cggctcacat cggcccgggt gaacgctggc aggtggcgac cgggatcgcg
     1141 gtcgagattc cggaaggtca cgccgggatg gtccttcccc gttcgggcct ggcccgtcgg
     1201 cacggcattt ccctggtcaa cgcgccgggc ctggtcgacg ccggctaccg cggtgaggtg
     1261 aaggtgctgc tgctgaacaa tgatccggcc gaggtattcc ggatcaaccc aggcgaccgc
     1321 atcgctcaga tggtcgtgat cccgcttgcc gcggtggaac cggtcgcgac cgactcgctg
     1381 tcggagaccg aacggggcga gggcggcttc ggctcgagcg gccgctagcc cttgcgggct
     1441 gccttgagcg ccgcttcgtg ggcggctgtt tcgagtgaat cttcgagcgg ctcgagttcg
     1501 ggccgctcgc cgtggtgatg ctcgagcccg gtcgcgatca ggtgatcggc cagccaggcg
     1561 ttcttcggga gcagggggcc gtgcaggtag g
//
LOCUS       DAOQMF010000533         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_60127_length_1588_cov_3.609263, whole genome shotgun sequence.
ACCESSION   DAOQMF010000533 DAOQMF010000000
VERSION     DAOQMF010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60127_length_1588_cov_3.609263"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLV77_08670"
     CDS             <1..520
                     /locus_tag="PLV77_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15961.1"
                     /translation="LPESIGVGVDNSKFAARRAAKVHPRASAVVADIWDGLPIKDQAA
                     GLILNVFSPRNGEEIERILAPGGRLVVVTPQPGHLAGLIGRFGMISVDPDKEERLERT
                     LGPLAKAAGSRNLEWEMKLDRAAVRDLIQMGPSAGRMEEAEMESALQALEEVTAVEAA
                     VTVTTTSSEERQ"
     gene            517..>1588
                     /locus_tag="PLV77_08675"
     CDS             517..>1588
                     /locus_tag="PLV77_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC15962.1"
                     /translation="MSIDRKGASDFTREAHVHDIRCLDFEDNESFEEAKRGFIAPVPD
                     GQILKDDGDFVFDPHRLAFASGDPEQPDTVNPSLWRQARLYADGGLFEVCDRIYQVRN
                     LDISNITFIEGDTGLIVVDPLVSAEVAKAALDLYFEHRGEKEVVAVIHSHSHVDHFGG
                     VRGVVDQADVDSGKVPIIAPEGFLEAAVSENVAAGNVMTRRAMYQFGILLPPDPQGTV
                     GSGLGIGTSLGTITLIPPTDLITETGETRKIDGLTFEFMLTPDTEAPAEMHWYIPELR
                     ALTAAENCTHTLHNTYPIRGAIVRDPQAWSMYLNDTIDRWADKSDVMYAMHHWPVWGK
                     DRVREMLEKGRDAYRYIHDETLR"
BASE COUNT          309 a          512 c          532 g          235 t
ORIGIN      
        1 cttgccggaa agcatcgggg tcggggtcga caactccaag tttgccgccc gccgggccgc
       61 gaaagtccac ccgcgggcca gcgccgtcgt ggccgacatc tgggacggcc tgccgatcaa
      121 agaccaggcg gcgggcctga tcctgaacgt cttttcgccc cgcaacgggg aggagatcga
      181 gcggattctc gcccccggcg gccggctggt cgtggtcaca ccgcaacccg gacacctggc
      241 cgggctgatc gggaggttcg gaatgatctc ggtcgacccg gacaaggagg agcggctcga
      301 gcggacgctc gggcctctcg ccaaggcagc cgggagccgg aatctcgaat gggaaatgaa
      361 gctggaccgg gcggcagtca gggatctgat ccagatgggg cccagtgctg gaagaatgga
      421 ggaagccgaa atggaatcgg cgctccaggc actggaggag gtgaccgcgg tggaggccgc
      481 ggtgaccgtc accacaacga gcagcgagga gaggcaatga gcatcgatcg caagggcgca
      541 tccgacttca cccgggaggc ccacgttcac gacatccgct gtctggactt cgaagacaac
      601 gagtcgttcg aggaagcaaa acgcgggttc atcgccccgg tcccggacgg ccagatcctg
      661 aaagacgacg gtgacttcgt attcgacccg caccggctcg ccttcgcctc cggggacccg
      721 gaacagccgg acaccgtgaa ccccagcctc tggcgccagg cccgccttta cgccgacggc
      781 ggcctcttcg aggtctgcga ccggatctac caggtgcgga acctggacat ctccaacatc
      841 accttcatcg aaggcgacac cgggctgatc gtggtcgacc cgctggtttc ggccgaagtg
      901 gcgaaggctg cgctggatct ttacttcgag caccggggcg agaaggaggt cgtcgcggtg
      961 atccattcgc acagccacgt cgatcatttc ggcggcgtgc ggggggtggt cgaccaggcg
     1021 gacgtggatt cgggcaaggt cccgatcatc gcgccggagg gcttcctcga agcggcggtc
     1081 tccgagaacg tggcggccgg caacgtaatg acccggcggg cgatgtacca gttcggcatc
     1141 ctgctgccgc cggaccccca gggcacggtc ggcagcggcc tcggcatcgg tacttcgctg
     1201 ggcacgatca ccctgatccc cccgaccgac ctgatcaccg agaccggcga gacccgcaag
     1261 atcgacgggc tgaccttcga gttcatgctg accccggaca ccgaggcccc ggccgaaatg
     1321 cattggtaca tcccggagct gagggcgctt accgcggccg aaaactgcac ccacaccctg
     1381 cacaacacct acccgatccg cggggcgatc gtgcgcgacc cgcaggcctg gtcgatgtac
     1441 ctgaacgaca cgatagatcg ctgggccgac aagtcggatg tgatgtacgc gatgcatcac
     1501 tggccggtct ggggcaagga ccgcgttcgg gagatgctcg aaaagggccg ggacgcatat
     1561 cgatacatcc acgatgaaac cctgcgct
//
LOCUS       DAOQMF010000534         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_60325_length_1586_cov_2.516003, whole genome shotgun sequence.
ACCESSION   DAOQMF010000534 DAOQMF010000000
VERSION     DAOQMF010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60325_length_1586_cov_2.516003"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLV77_08680"
     CDS             <1..318
                     /locus_tag="PLV77_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15963.1"
                     /translation="NIPGVRARITTAGPAVVRISSKLIYHLNGRQGQISYKQRQFAVA
                     GHSVNYKVAIPGRLRARLKPKKQVRFVITYASKARNPQCTKFARKKTRNLTTRIVWVI
                     PNA"
     gene            352..>1586
                     /locus_tag="PLV77_08685"
     CDS             352..>1586
                     /locus_tag="PLV77_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC15964.1"
                     /translation="MNLRLLTTLAAAQFLMVLDQAVMNVSISQLVEDFDTTVTTIQAI
                     IAFYALVMAGLMLIGGKLGDIYGRKRIFKIGLVIYACGSALTAASWSVASLAVGWSVL
                     EGIGAAMVMPAMVALVAGNFEGKNRAIAYGVLGGMAGVGIAVGPILGGWMTTDFTWRY
                     VFLGEVALAAAIFIGTRWMLEPPAPGRRPRLDVVGGVLSALGLAVMVMGVLQASNWGW
                     LRPMNSPVVIFGFSLTPFVIVAGLILLGLFASWERRREERGEDPLVHLALFRNVPLRS
                     GLSMMLAQNLILMGIFFTIPLYLQVVLGFDALDTGVRMLPTSAGLFFTALAGSALASR
                     FAPKKLVRLGLVVVFVSALLLVGTIEPSLDNTDFLVAMGVLGVGMGLIVSQLGNVAQS
                     TVGDEDRRLGDVSELGNDQP"
BASE COUNT          234 a          530 c          517 g          305 t
ORIGIN      
        1 aacatccccg gggtgagggc gcggatcacc actgccggcc ccgccgtggt caggatttcc
       61 tcgaagctga tctaccacct gaacggcagg cagggccaga tcagttacaa gcagcggcag
      121 tttgccgtcg ccgggcattc ggtcaactac aaggtcgcga ttccgggcag gctgcgagcc
      181 aggctcaagc cgaagaagca ggtccgcttc gtgatcacct acgccagcaa ggctcgcaat
      241 ccccaatgca cgaagttcgc ccggaagaag acccggaacc tgaccacgcg gatcgtctgg
      301 gtcatcccga acgcatgatg tcccgtcggc tggctgacgg ataatcacgc catgaacctg
      361 cgattgctga cgacgctggc cgctgcccag ttcctgatgg tgctcgacca ggcggtgatg
      421 aacgtctcga tctcccagct ggtcgaggac ttcgacacca cggtcaccac gatccaggcg
      481 atcatcgctt tctacgcgct ggtcatggcc ggcctgatgc tgatcggcgg caagctcggt
      541 gacatctacg ggcgcaagcg gatcttcaag atcggcctcg tcatctacgc ctgcggctcg
      601 gcgcttaccg ccgcttcctg gagcgtcgcc agcctggccg tgggctggtc cgtcctcgag
      661 gggatcggtg ccgcgatggt catgccggcg atggtcgcgc tcgtcgccgg caacttcgaa
      721 ggcaagaacc gggcgatcgc ctacggggtg ctgggcggca tggccggggt cgggatcgcg
      781 gtcggcccga tcctcggcgg ttggatgacc accgacttca cctggcggta cgtcttcctc
      841 ggcgaggtgg ccctggccgc ggcaatcttc atcggaaccc gctggatgct cgaaccgccg
      901 gcgcccggcc ggcgtccgcg gcttgacgtg gtcggcgggg tgctttccgc actcgggctg
      961 gcggtcatgg tcatgggcgt gctccaggcc agcaactggg gctggctcag gccgatgaac
     1021 tctccggtcg tgatcttcgg cttctcgctg accccgtttg tgatcgtcgc cggcctgatc
     1081 ctcctcggtc tgttcgccag ctgggagcgg cgccgggaag aacgcggcga ggatccgctg
     1141 gttcacctcg ctctcttccg caacgtccct ctgcgttcgg gcctctcgat gatgctggcc
     1201 cagaacctga tcctgatggg catcttcttc accatcccgc tctacctgca ggtcgtgctc
     1261 ggcttcgacg cgctcgacac cggggtgcgg atgctgccga cctcggccgg gctcttcttc
     1321 accgccctgg ccggttccgc gctggcgtcc cggttcgcgc cgaagaagct ggtccggctc
     1381 ggcctcgtcg tggtcttcgt ttccgccctg ctcctggtcg ggacgatcga gccctcgctg
     1441 gacaacaccg acttcctggt tgcgatgggc gtgctcgggg tcggcatggg gctgatcgtt
     1501 tcccagctcg gaaacgtcgc ccagtccacg gtcggggacg aagaccgcag actgggcgac
     1561 gtttccgagc tgggaaacga tcagcc
//
LOCUS       DAOQMF010000535         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_60535_length_1583_cov_3.455497, whole genome shotgun sequence.
ACCESSION   DAOQMF010000535 DAOQMF010000000
VERSION     DAOQMF010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60535_length_1583_cov_3.455497"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PLV77_08690"
     CDS             <1..1143
                     /locus_tag="PLV77_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNC15965.1"
                     /translation="VETADPDHVPATRAEVDRYEKAAVLLERAGGSPLAAFAAVRDRN
                     VSLIPSSATHAVLPLLATRAGLDLQIDAGLASHRRRFGPSSGFWLPECAYRPGLEDAL
                     ARHRIEWFCVDQSRHESGDAALAPVRTPAGPLAFTIDWPAISWLWDMSGYPSWGGYLD
                     FHRLSMNGVRLFAIDGGPYEPEAARQRADRQAGEFLDSVRERLEEYRQRTGQRGLMTF
                     AIDTELLGHWWSEGPDWLAAVLDQAGERGVRAVNLEQAAREHGPSARQSSLKASTWGE
                     AKDFHTWDSPAVADLAWASRGLELRVGEAIGSLPREQAERAVRELLMVQSSDWAFLDH
                     RGQAGDYPFERATGHAESAFEALQSPIGSRLRGLAPDLSLTPFLTP"
     gene            1159..>1583
                     /locus_tag="PLV77_08695"
     CDS             1159..>1583
                     /locus_tag="PLV77_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC15966.1"
                     /translation="MPRILIVTWEYPPLIEGGLARHVRKLSEALVGLGVEVHVLTRGG
                     EQATIEEEMAGVQIHRIVEPERPTDLAEFVTWVERMNADMLAAGVELGDRFDFDLVHG
                     HDWLVAGACDHLARRFNAPLVTTIHATEFGRHQGWVDKHP"
BASE COUNT          228 a          548 c          533 g          274 t
ORIGIN      
        1 gttgaaacgg cggatccgga tcacgtgccg gcgacccggg cggaagtcga ccgttacgag
       61 aaggcggcgg tcctgctcga gcgggccggc ggcagtccgc tggccgcgtt cgccgcggtc
      121 cgggaccgga atgtttccct gatcccctcc agcgccactc atgcggtcct gcccctgctg
      181 gcgacccggg ccgggctcga cctccagatc gacgccggtc tcgcctctca ccggcgccgc
      241 ttcggccctt catccggatt ctggcttccc gaatgtgcct atcgtccggg tctggaggat
      301 gctcttgccc ggcaccggat cgagtggttc tgcgtcgacc agagccggca cgaatccggc
      361 gacgccgccc tcgcgccggt ccggaccccg gccggtccgc tcgccttcac gatcgactgg
      421 ccggcgatct cctggctttg ggatatgtcc ggctacccgt cctggggcgg ctacctggac
      481 ttccatcgcc tttccatgaa cggggttcgt ctctttgcga tcgatggtgg tccctacgaa
      541 ccggaggcag cccgccagcg ggcggaccgg caggcgggcg agttcctcga ctcggtccgg
      601 gagcgccttg aggagtaccg acagcgcacc gggcagcggg ggctgatgac ctttgcgatc
      661 gacaccgaac tgctcggaca ttggtggtcg gagggtccgg actggctggc cgcggtcctt
      721 gaccaggccg gggagagggg ggtccgtgcc gtcaacctcg aacaggcggc gagggaacat
      781 ggcccgtcgg cccgccaaag cagtctgaaa gcttcaacct ggggcgaggc taaggacttc
      841 cacacctggg attcgcctgc cgtggccgac ctcgcctggg cctcgcgggg cctggagctg
      901 agggtggggg aggcgatcgg gtccctgccc cgggaacagg ccgagcgggc cgttcgcgag
      961 ctcctgatgg ttcagtccag cgactgggcc ttcctcgacc accgcggcca ggccggcgac
     1021 tatcccttcg agcgggccac cggccatgcg gaaagcgcct tcgaagcctt acaatccccg
     1081 atcggttcgc gtttgcgcgg ccttgctcca gacctgagtc tcacaccgtt tctcacgccc
     1141 tagccttcag ccaccacctt gcctcgcatc ctcatcgtca cctgggagta cccgccgctg
     1201 atcgagggcg gcctggcccg gcatgtgcgt aagctctccg aagcactggt cgggctcggg
     1261 gtcgaggttc acgtgctgac ccgcggcggt gagcaggcga ccatcgagga agagatggcc
     1321 ggggtccaga tccaccggat cgtcgaaccg gaacgcccga ccgacctggc cgagttcgtg
     1381 acctgggtcg agcgcatgaa cgccgacatg ctcgccgccg gagtggagct cggcgaccgt
     1441 ttcgattttg atctggttca cggtcacgac tggctggtcg ccggtgcctg tgatcacctc
     1501 gcccggcgct tcaacgctcc gctggtcacg acgatccacg cgaccgagtt cggccgccac
     1561 cagggctggg tcgacaagca tcc
//
LOCUS       DAOQMF010000536         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_60939_length_1578_cov_3.003940, whole genome shotgun sequence.
ACCESSION   DAOQMF010000536 DAOQMF010000000
VERSION     DAOQMF010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60939_length_1578_cov_3.003940"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1578)
                     /gene="acnA"
                     /locus_tag="PLV77_08700"
     CDS             complement(<1..>1578)
                     /gene="acnA"
                     /locus_tag="PLV77_08700"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNC15967.1"
                     /translation="DAYARLQGTFHTADSGDPVFSDTLELELDSVVPSIAGPKRPQDR
                     VSLSDAKPAFLEALCEFDEDASNQLSAYDEALEESFPASDPPGEDHVTSDLGKPAGAP
                     TRASTRVAEQRCSEDMIGVTLEDGTSFDLDHGRVVIAAITSCTNTSNPYVMVGAGLLA
                     RNALAKGLSRKPWVKTSLAPGSTVVTDYLDNAGLTQYLDQLQFNLVGYGCTTCIGNSG
                     PLAPEISAAIDEKDLVVCSVLSGNRNFEGRISQDVKANYLASPPLVVAYALAGRMDID
                     IQKDPLGNDADGNPVYLDDIWPDPAEVAQVVGDSIRREMFEKNYGDVFKGDENWQKVS
                     VPEGDRYTWPDSTYVRKPSFFEDMPADPPGLMPIEGARVLARLGDSITTDHISPAGAI
                     KKDSPAGAWLIEQGVVPADFNSYGSRRGNHEVMVRGTFANIRLRNQLVEKSGGFTRHF
                     PDGEETSIYEASSQYQADGIPLVVLAGKEYGSGSSRDWAAKGTRLLGVRAVIAESYER
                     IHRSNLIGMGVVPLQYP"
BASE COUNT          257 a          483 c          558 g          280 t
ORIGIN      
        1 ccgggtactg gagcggaacc acacccatgc cgatcaggtt cgagcggtgg atccgctcgt
       61 agctttcggc gatgaccgcg cggaccccga gcaggcgggt ccccttggcg gcccagtcgc
      121 gggatgaacc cgacccgtac tccttgccgg ccaggacgac cagcgggatg ccgtcggcct
      181 ggtactggct ggaagcctcg tagatcgaag tctcctcgcc gtccgggaag tggcgggtga
      241 agccgccgga cttctcgacc agctggttgc ggaggcggat gttggcgaag gtgccacgca
      301 ccatcacctc gtggttgccg cgccgtgagc cgtatgagtt gaagtcggcc gggacgactc
      361 cctgctcgat cagccaggca ccggccgggc tgtccttctt gatcgctccg gcgggcgaaa
      421 tgtgatcggt ggtgatcgag tcgcccagcc tggcgagcac ccgggctccc tcgatcggca
      481 taaggccggg cgggtcggcc ggcatgtcct cgaagaagga gggcttgcgg acataggtcg
      541 aatccggcca ggtgtagcgg tcaccctcgg gcaccgagac tttctgccag ttctcatcgc
      601 ccttgaagac atcgccgtag ttcttctcga acatctcgcg tcggatcgaa tcgccgacca
      661 cctgagcgac ctcggcgggg tcgggccaga tgtcgtcgag gtaaaccgga ttgccgtcgg
      721 cgtcgttgcc cagcgggtcc ttctggatgt cgatgtccat ccggccggcc agtgcgtacg
      781 ccaccacgag tggcggcgag gcgaggtagt tcgccttgac gtcctggctg atccggccct
      841 cgaaattgcg gttgccggaa aggaccgagc agaccaccag gtccttctcg tcgatcgccg
      901 ccgagatctc gggggctagc ggtccggagt tgccgatgca ggtggtgcag ccgtaaccga
      961 caaggttgaa ctggagctgg tccaggtact gggtcaggcc ggcattgtcg aggtagtcgg
     1021 tgaccacggt cgagccggga gccagcgagg tcttcaccca gggcttgcgg gaaagaccct
     1081 tggccagggc attcctggcc agcaggccgg ccccgaccat cacatacggg ttcgaggtgt
     1141 tggtgcagga agtgatcgcg gcgatcacga cgcgaccgtg gtcgaggtcg aaggaggtgc
     1201 cgtcctcgag cgtgactccg atcatgtctt cgctgcaacg ctgctcggca accctggtgc
     1261 tggctctggt cggcgcgccg gccggcttgc cgagatcact cgtgacatgg tcttcacccg
     1321 ggggatcgga agcggggaag gattcctcca gcgcctcgtc ataggcgctg agctggttcg
     1381 aggcgtcctc gtcgaattcg cagagcgcct cgagaaaggc aggcttggcg tccgagagcg
     1441 agacccggtc ctgcggccgc ttcgggccgg cgatcgatgg caccaccgag tcgagttcga
     1501 gctcgagcgt gtcggagaag acgggatccc cggaatcggc ggtgtggaag gtgccctgca
     1561 ggcgggcgta ggcatcga
//
LOCUS       DAOQMF010000537         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_60954_length_1578_cov_2.449770, whole genome shotgun sequence.
ACCESSION   DAOQMF010000537 DAOQMF010000000
VERSION     DAOQMF010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60954_length_1578_cov_2.449770"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLV77_08705"
     CDS             complement(<1..325)
                     /locus_tag="PLV77_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15968.1"
                     /translation="MATHCPECGFVNPEAANYCQKCGTFIGRREGGDVQGTSTYQVGE
                     TGEIEAVDIDAEVERTGAALVIRSGGGRAGETFPMEEERVAIGRSPDAGVFLDDVTVS
                     RNHALL"
     gene            complement(368..1030)
                     /locus_tag="PLV77_08710"
     CDS             complement(368..1030)
                     /locus_tag="PLV77_08710"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNC15969.1"
                     /translation="MGDSAPFGKGRVRKLFGLDRTGAEPAPTRAGQPLRPFTIPNFIG
                     YLRLLGIPLFLYVAFSSDDGRDWTAALLFFLIAGGDYVDGFLARVTGQYSRLGALMDP
                     VIDRLTIFSGLVVCWHFELLPRWLLALLLVRELATLLLARWGLSHGVDIEVNWFGRVA
                     VFPVMGALFLALIWDSWVPAVMLGVGVVLGVIATWLYARVGRDRIHALEAAARPEETA
                     QP"
     gene            complement(1030..>1578)
                     /locus_tag="PLV77_08715"
     CDS             complement(1030..>1578)
                     /locus_tag="PLV77_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNC15970.1"
                     /translation="DTTDDGHSITLEPAIEIGNIFKLGTRYTEPLGATFLDENGEEKP
                     VIMGCYGIGPARIVAAAAEQYADENGLSWPVSLAPWDIQLVSLSKSDEPERVEADRLY
                     EELLEQGFDVLYDDRDAGPGQKLTDAELLGCPLRVVVGRRGLADGIYEVSERRSGEVH
                     RVPVEGGAAGIAGLYAAISGSD"
BASE COUNT          278 a          540 c          508 g          252 t
ORIGIN      
        1 ccagcagcgc gtgattgcgg gaaaccgtga cgtcatcgag gaagacgccg gcatcgggac
       61 tgcgcccgat cgccacccgt tcctcttcca tcgggaaggt ctctccggcc cgtccgccgc
      121 cgctgcggat gaccagggcg gcaccagtgc gttcaacctc ggcgtcgatg tcgactgctt
      181 cgatctcgcc ggtttccccg acctggtagg tcgaggttcc ctgtacgtct cccccttcgc
      241 ggcggccaat gaaggttccg cacttctggc agtagttggc ggcctcgggg ttgacaaatc
      301 cgcattcggg gcaatgggtc gccatagcgg ccattatagg ggccgaccct aaccttcaac
      361 tcaggcttta cggttgggcg gtttcttccg gcctggccgc tgcttccagg gcgtgaatcc
      421 ggtcccggcc caccctggcg taaagccagg tcgcgatcac tccgagaacc acgccgacgc
      481 cgagcatcac ggccgggacc cagctgtccc agatcagggc caggaacagg gcgcccatca
      541 cggggaagac ggcgacccgg ccgaaccagt tcacctcgat gtctaccccg tgcgagagcc
      601 cccagcgggc cagcagcagg gtggccagtt cccggaccag aagcagcgcc agaagccagc
      661 gcggcagcaa ctcgaaatgc cagcagacga ccaggccgga gaagatcgtg agccggtcga
      721 tcaccgggtc catcagggcc ccgaggcggc tgtactgacc ggtcacccgg gccaggaagc
      781 cgtcgacgta atcgcccccg gcgatcagga agaagagcag tgcggcggtc cagtcgcgac
      841 cgtcatccga gctgaaggcg acatagagaa agagcgggat gccgagcagc cggaggtagc
      901 cgatgaagtt ggggatcgtg aacggccgaa gcggctgccc ggcccgggtc ggggccggtt
      961 cggccccggt ccggtcgaga ccgaaaagtt tgcggaccct gcccttgccg aagggagcgg
     1021 agtcccccat cagtccgatc cggagatcgc tgcgtacagc ccggcaatac cggcggcacc
     1081 accctcgacc gggacacgat gcacttcacc gctccgcctt tcgctgactt cgtagatgcc
     1141 gtcggcgagc cctcgccggc cgaccacgac cctgagcgga cagcccagca gctcggcatc
     1201 ggtcagcttc tggccgggcc ctgcgtcccg gtcgtcgtag agcacgtcga agccctgctc
     1261 gagcagttcc tcatagagcc ggtccgcctc gacccgctcc ggctcgtcgg atttcgaaag
     1321 cgacaccagc tgaatgtccc agggtgccag gctgaccggc caggaaaggc cgttctcgtc
     1381 ggcatactgc tcggccgcgg cagccacgat ccgcgccggt ccgatcccgt aacagcccat
     1441 gatcaccggt ttctcctccc cgttctcatc gaggaaggtg gctccgagcg gctctgtgta
     1501 gcgagtgccc agcttgaaga tgttgccgat ctcgatcgcc ggctcgaggg tgatcgagtg
     1561 gccgtcatcg gtggtgtc
//
LOCUS       DAOQMF010000538         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_61450_length_1571_cov_4.870053, whole genome shotgun sequence.
ACCESSION   DAOQMF010000538 DAOQMF010000000
VERSION     DAOQMF010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61450_length_1571_cov_4.870053"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLV77_08720"
     CDS             complement(<1..363)
                     /locus_tag="PLV77_08720"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNC15971.1"
                     /translation="MSTTPQLPTTLDQEIIRCFEPEIQAEMTDPERLAKIQQEFEEGF
                     EVMKPVGPAVCVFGSARTRPGHPEYEQARRVGAGIARQGYAVITGGGPGSMEAANRGA
                     KDEEGLSVGLNIELPHEQG"
     gene            complement(374..>1571)
                     /locus_tag="PLV77_08725"
     CDS             complement(374..>1571)
                     /locus_tag="PLV77_08725"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNC15972.1"
                     /translation="EAGATTVHWTEDFSPFATGRDRRVGSALAGAGITAIPHPGACIA
                     DRPQEIQTGQGRPYTVFSPFNRAWQEAPRRPAEARPESLKLAKRSGGGQFEAGELPDL
                     AELGLAADDSDWQEAFEPGEAAGRVAAKEFLNRGLSEYDERRDNPAGGSSRLSPWIRW
                     GCLSPLELEAKLGRRQGEGPRSFRSELAWRDFYASVIASHPEVTRLEYQERYRGTLDW
                     QSDEGLLAAWREGRTGYPLVDAGMRQLVAEGWMHNRVRMVVASFLTKDLHLDWREGER
                     HFMELLIDGDVASNNGGWQWVASTGTDPAPYFQRMFNPMTQQEKFDPEGEYVRRWVPE
                     LRKVPQGHLVRPWKMTGEEQEEAGCLIGKDYPEPVVDHAEERRFAIERYRAAADADPS
                     DGDWRG"
BASE COUNT          234 a          558 c          491 g          288 t
ORIGIN      
        1 tccctgctca tggggcagtt cgatgttcaa cccgaccgag agtccttcct cgtccttggc
       61 gccgcggttc gccgcctcca tcgaacccgg accgccgccg gtgatcaccg catagccctg
      121 gcgggcgatg ccggccccga cccgacgggc ctgttcgtac tcggggtggc cgggcctggt
      181 cctcgccgac ccgaacacgc agaccgccgg cccgaccggc ttcatcacct cgaatccctc
      241 ctcgaattcc tgctggatct tcgcgaggcg ttcgggatcc gtcatctcgg cctgaatctc
      301 gggctcgaaa cagcggatga tctcctggtc gagagtggtg ggcagctgtg gagtcgtact
      361 catcggcggc agcctatccc cgccagtccc cgtccgacgg gtctgcgtcg gccgcggcac
      421 ggtaccgctc gatcgcgaat cgccgctcct ccgcgtgatc gactaccggc tccggatagt
      481 ccttgccgat caggcaaccg gcctcttcct gctcttcacc ggtcatcttc caggggcgaa
      541 ccaggtgtcc ttggggaact ttccgcaact ccggcaccca acgccgcaca tattcccctt
      601 cggggtcgaa cttttcctgc tgggtcatcg gattgaacat gcgctggaaa tagggggcgg
      661 ggtcggtccc ggtcgaagcg acccactgcc agccgccgtt gttcgaggcg acgtcgccgt
      721 cgatcaggag ttccatgaaa tgcctttcgc cctctcgcca gtcgaggtgg agatccttgg
      781 tcaaaaagga agcgacgacc atccgcaccc ggttgtgcat ccagccttcg gccaccagct
      841 ggcgcatgcc ggcgtcgacc agggggtagc cggtacggcc ctcccgccag gctgcgagca
      901 acccctcgtc gctttgccag tcgagcgtgc cccggtatcg ctcctggtac tcgagcctgg
      961 tcacctccgg atggctggcg atcaccgagg cgtagaaatc ccgccaggca agctcggaac
     1021 ggaagctgcg cggaccttcg ccttgccgcc gacccagttt ggcttcgagc tccaggggcg
     1081 aaaggcagcc ccagcggatc cagggagaaa gccgggagga tcccccggcc ggattgtcac
     1141 gccgctcgtc atattcggaa agtccccggt tgagaaactc ttttgcggct acccggccgg
     1201 ccgcttctcc cggctcgaag gcttcctgcc agtcggaatc atctgccgca agtcccagct
     1261 cggctaggtc gggcagttca ccggcttcaa actggccacc cccggacctc ttcgccagct
     1321 tcaggctctc cggccgcgct tcggccgggc gccgcggcgc ttcctgccag gcgcggttga
     1381 aagggctgaa caccgtgtag gggcggccct gcccggtctg gatttcctgt gggcggtcgg
     1441 cgatgcaggc accggggtga gggatcgcgg tgatcccggc cccggccagt gcgctgccga
     1501 ccctccggtc cctcccggtc gcgaaggggg aaaagtcctc agtccagtgg acggtcgtcg
     1561 ccccggcttc g
//
LOCUS       DAOQMF010000539         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_61456_length_1571_cov_4.083113, whole genome shotgun sequence.
ACCESSION   DAOQMF010000539 DAOQMF010000000
VERSION     DAOQMF010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61456_length_1571_cov_4.083113"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..>1571)
                     /locus_tag="PLV77_08730"
     CDS             complement(221..>1571)
                     /locus_tag="PLV77_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC15973.1"
                     /translation="LSPEQVVTIGPELRAALQDGTGNRGIKGEAEALLLPADAEEVAE
                     VMAWCYGHGVPLTVRGGGTGLAGGAVPDGGVVLSLERLARVRSIDPELWRMEVEAGVR
                     TATVHRIALENGLMFPPDPGASEQSTIGGNIATNAGGPHAFRYGVTGAWVTGIEAVVP
                     PGEVLSSGGPLRKNVAGLDLTDLMIGSEGTLGIVTSAWLRLVPAPEERGVVVGFYPDT
                     ASGCEAIGMIMGSGIQPTALEFLDRGALESSLGSFPSEAPEQAGFAVIAEADGSAGEV
                     NQAVGDLEEAMAGGALQPPVRITDHVAIRNFWAWRDGVSIAVTAQRGGKISEDVVVPV
                     ESLAAAVDETVEIGRRHDLPACSWGHAGDGNLHSSFLVDLADDDQMKRGGAAAAEIFE
                     MAIRLGGSISGEHGIGSLKTGHVEAALGPVAVRLQAEIKRVFDPKLLLNPGKKIPLP"
BASE COUNT          249 a          556 c          506 g          260 t
ORIGIN      
        1 cattcgggca gcttgaagct catgccgtcc tcaccgccgg tgatctcgga catcactgct
       61 ccttgctgtc ggcggcatcg tgcggtgccg cacgggattg gaagtctgct tttcttccgg
      121 aaaagggcag acccgagtgt aggagcaaat ctcgtcttct ccgcttcccg agcgaaagtt
      181 cagggttttc ccgcagtctc ctgaagggcg ggagcgaggc tcagggcagc gggatcttct
      241 tgcccgggtt gagcagcagc ttggggtcga agactcgttt gatctccgcc tgcagccgca
      301 ccgcgaccgg cccgagggca gcctccacat gtccggtctt gagcgagccg atgccatgct
      361 cgccgctgat cgacccgccc aggcggatcg ccatctcgaa gatctcggcc gccgccgcgc
      421 cgccccgctt catctggtca tcgtcggcca ggtcgacgag gaagctggaa tgcaggttgc
      481 cgtccccggc gtgaccccag gaacaggccg gcaggtcgtg acggcggccg atctcgaccg
      541 tctcgtcgac cgccgcagcc agcgactcga ccggaaccac gacatcctcg gaaatcttgc
      601 cgccgcgctg ggcggtgacg gcgatcgaaa ctccgtcccg ccaggcccag aagttccgga
      661 ttgcgacatg gtcggtgatc ctgaccggcg gctgaagtgc ccctccggcc atcgcctctt
      721 ccaggtcccc gaccgcctgg ttcacctcgc cggcggaacc gtcggcctcg gcgatcacgg
      781 caaaacccgc ctgctccggt gcctcggaag gaaaggaacc gaggctggat tcgagcgcgc
      841 cgcggtcgag gaactcgagc gccgtcggct ggatcccgga acccatgatc attccaatcg
      901 cctcgcagcc ggaggcggtg tccgggtaga agccgaccac gacgccgcgt tcctcggggg
      961 ccggcaccag ccgcagccag gctgaggtga cgatcccgag ggttccttcc gaaccgatca
     1021 tcaggtcggt caggtcgagg ccggcgacgt tcttcctcag cggcccgccg gaggaaagga
     1081 cctctcccgg tggcacgacc gcctcgatgc cggtcaccca ggccccggtc accccgtacc
     1141 ggaatgcgtg agggccgccg gcgttggtgg cgatgttgcc gccgatcgtg gactgttcgc
     1201 tggcccccgg gtcgggaggg aacatcagtc cgttctcgag cgcgatccgg tgcactgtgg
     1261 ccgtccggac cccggcctcg acttccatcc gccagagctc gggatcgatc gaacgaaccc
     1321 gggctagccg ttccaggctg agtacaaccc cgccatccgg cacggcccca ccggccagtc
     1381 cggtgccacc cccgcgcacg gtcagcggca cgccatgccc gtagcaccag gccatgacct
     1441 cggccacttc ttcagcgtcg gcgggcagga ggagagcctc ggcctcgcct ttgatgccgc
     1501 ggttgccggt tccgtcctgc agggcagctc tcaattcggg gccgatagtg accacctgtt
     1561 caggcgaaag c
//
LOCUS       DAOQMF010000540         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_61458_length_1571_cov_3.932058, whole genome shotgun sequence.
ACCESSION   DAOQMF010000540 DAOQMF010000000
VERSION     DAOQMF010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61458_length_1571_cov_3.932058"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLV77_08735"
     CDS             <1..1049
                     /locus_tag="PLV77_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC15974.1"
                     /translation="FPAQLTALRATGLVIAVLIIAFAIIRRRELRNADVILLLLAGIG
                     LAIVSGTELTDRLLSALSFNRGNGGRILGLAVIAIFILFLMALRALSVGARNARQLSA
                     VLEGISSEQFRVADNRKKFKGKVAIVIPAYNEADNIGYVLDQIPAEVCGLPTATLVVD
                     DGSRDGTEEVAEAHGAVVIRHVVNRGGGAALRTGYRLMVDSEAAIVVTLDADGQHLPA
                     EMPNLVQPVLDGKADVAHGSRVLGHAEDNHFAREMGIVFFNRLVSFITRTHVTDCSNG
                     YRAVRTSVLPTLVLKQEQFHTSEFMIEAIKRGVPAVEVPVTVEQRLHGHSKKPAVFRY
                     GMGFANAIVRTWLR"
     gene            complement(1284..>1571)
                     /locus_tag="PLV77_08740"
     CDS             complement(1284..>1571)
                     /locus_tag="PLV77_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15975.1"
                     /translation="VGVGYEYVFIALLFGIATGIIGRAKGGSFLIWLVVGTILPGLGL
                     IAVFMMRNEVNEPERRCENCGKVLKLYVQVCPRCGYDLYLPPPEETRMPPR"
BASE COUNT          273 a          547 c          486 g          265 t
ORIGIN      
        1 tattccctgc ccagctcacc gccctgcggg cgaccggcct cgtgatcgcg gtcctgatca
       61 tcgccttcgc gatcatcagg cgtcgcgagc tgaggaacgc cgacgtgatc ctgctgctcc
      121 tggccggaat cggcctggcc atcgtctccg gcaccgaact caccgaccgc ctgctctccg
      181 ccctctcctt caaccgcggc aacggcggcc ggatcctcgg tctcgcggtc atcgcgatct
      241 tcatcctctt cctgatggcg ctgcgggcgc tctcggtcgg agccagaaac gcccgccagc
      301 tttcggccgt actcgaaggc atctcttccg agcagttccg ggtcgccgac aaccgcaaga
      361 agttcaaggg caaggtggcg atcgtgatcc ccgcctacaa cgaggcggac aacatcggat
      421 acgtgctcga tcagatcccg gccgaggtct gcggacttcc gaccgccacg ctggtcgtcg
      481 acgacggttc acgcgacggc accgaggaag tcgccgaagc ccacggggcg gtggtgatcc
      541 gccatgtcgt aaaccgcggt ggcggcgcag cactgcgcac cggctaccgg ctgatggtcg
      601 attccgaagc ggcgatcgtg gtcaccctcg acgccgacgg ccagcacctc ccggccgaga
      661 tgccgaacct ggtccagccg gtcctcgacg gcaaggccga cgtcgcccat ggcagccggg
      721 tgcttggtca cgccgaggac aatcacttcg cccgggaaat gggcatcgtc ttcttcaacc
      781 ggctggtctc cttcatcacc cggacccacg tcaccgactg ttcgaacggc taccgggcag
      841 tgcgaacttc ggtcctcccc accctggtgc tcaagcagga gcagttccac acttccgagt
      901 tcatgatcga ggcgatcaag cggggcgtgc ccgcggtcga ggtcccggtc acggtcgaac
      961 agcgcctgca cggccactcg aagaagcccg ccgtcttccg ctacggaatg ggcttcgcca
     1021 acgccatagt ccgaacctgg cttcgctgac ttaactgcaa gtcgaacggg gtccccgacg
     1081 ccacggctgc caggacccgg tcgagccccg aaggtgccgc aggtttcaga ccggatcggg
     1141 cccaaacctg cggcacctca atcgtcgacc cgggtccaac ccggtcagcg gacggcgaga
     1201 tcccattttg cgccacatac cggcacaaag tgggcactcg atgattcagc cccacgccaa
     1261 cgctgcgggc cgttggccgc gccttaccgg ggcggcattc gggtttcttc tggtggtggc
     1321 aggtagaggt cgtagccgca gcgggggcag acctggacgt agagcttgag gaccttgccg
     1381 cagttctcgc agcggcgttc cggttcgttg acttcgtttc gcatcatgaa gacggcgatc
     1441 aggccgagcc cgggcaggat cgtgccgacg accagccaga tcaggaagga gccgcccttg
     1501 gcgcggccga tgatcccggt cgcgattccg aagagcaggg cgatgaagac gtattcgtaa
     1561 cccacgccga c
//
LOCUS       DAOQMF010000541         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_61535_length_1570_cov_4.008581, whole genome shotgun sequence.
ACCESSION   DAOQMF010000541 DAOQMF010000000
VERSION     DAOQMF010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61535_length_1570_cov_4.008581"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLV77_08745"
     CDS             <1..517
                     /locus_tag="PLV77_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15976.1"
                     /translation="SKDFISTAVGSAGSTINGNDGLDVLYAQDRVTVNGGDDTDIFRV
                     VDPCLGAELRGNAGTDSVVFAGAPNGVKASLAGGYAEWRNGGCGSDTDIARDIEKLEG
                     TDYNDWLIVGKRNSSQDGRSVLFGRGGIDILDAKNGSVDQITTGAGGRNNTVIADKND
                     RITWGYGLGDY"
     gene            656..>1570
                     /locus_tag="PLV77_08750"
     CDS             656..>1570
                     /locus_tag="PLV77_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC15977.1"
                     /translation="MFEGKSAGRLVRILVLALALLVAAAGQLFLTARAEARPSSCFGK
                     TVNRVVKSSNTTVHLAYRDVAWVEGTGVTVIGKPYSRICAGGGRQVIHAGKGTSFTDA
                     GPGNDRIVLHPKSNDNQVHGGAGDDEIDGSSGNDFIYGSPRRLPSGVSDSDDLVGKGG
                     NDHIYDYGGTGNQLRGLTGSDRLYSLGRAVSQVHGGNGTDFIFSNGGKSRSGVIEKVF
                     GEQGNDRIRADRTPNNGPAYLDGGEGDDWMTGSKRDDIFIYNAGIKKLRGLGGDDLFV
                     TTGRGLGRINGGGGTDTISFAALTSSNAY"
BASE COUNT          319 a          504 c          511 g          236 t
ORIGIN      
        1 cagcaaggac ttcatttcga ccgcggtcgg cagcgccggc agcacgatca acggcaacga
       61 cggcctcgac gtcctttacg cacaggaccg ggtcaccgtc aacggcggcg atgacacaga
      121 catcttccgg gtcgtcgatc cctgcctcgg agcggagctg cgtggcaatg ccggcaccga
      181 cagcgtggtg ttcgcgggtg ccccgaacgg cgtcaaggca agcctggcag gcggttacgc
      241 ggagtggcga aacggcggct gtggcagtga cacggacatc gccagggaca tcgagaagct
      301 cgaaggaacc gactacaacg actggctgat cgtcggcaag cggaactcct cccaggacgg
      361 gcggtccgtg ctcttcggtc gcgggggcat cgacattctg gacgcgaaga acggcagcgt
      421 ggaccagatc accaccggcg ccggcggccg taacaacacg gtgatcgccg acaagaacga
      481 ccggatcacc tggggctacg gactgggcga ttactgaggc cccgccccgg atcgggccgg
      541 ttcgcaggcg ggattgttta cttccgttcg cctgcgggaa caacagtgac ttcgcaactg
      601 ttacaggttg tcacgaggag ctccctggac atgagtaccc acgacgggag atcaaatgtt
      661 cgaaggtaaa tctgcaggtc gtctggtcag gatcctggtt ctggccctgg cccttctggt
      721 cgcggcggcc ggtcaacttt tcctgaccgc cagggccgaa gcccggccct ccagctgctt
      781 cggaaagacg gtcaaccgcg tcgtcaagtc cagcaacacg acggttcacc tggcctaccg
      841 ggatgtcgcc tgggtcgagg ggaccggcgt caccgtgatc ggcaagccct acagccggat
      901 ctgcgctggc ggtggccgtc aggtcatcca tgccggcaag ggcacctcct tcaccgacgc
      961 cggaccgggc aacgacagga tcgtcctgca cccgaagagc aacgacaacc aggttcacgg
     1021 cggcgccggt gacgacgaga tcgacggctc gagcggaaac gacttcatct acgggtctcc
     1081 ccgtcgcctg ccctccgggg tcagcgacag cgacgacctg gtcggcaagg gcggcaacga
     1141 tcacatttac gactacgggg gaaccggcaa ccagcttcgc ggcctgaccg gctccgaccg
     1201 cctgtacagc ctcggcaggg cggtctccca ggtgcacggc ggcaacggca ccgacttcat
     1261 cttctccaac ggcggcaaga gccgaagcgg agtgatcgag aaagtgttcg gcgagcaggg
     1321 caacgaccgc atccgggccg accggacacc gaacaacggc cccgcctatc tcgacggagg
     1381 cgagggtgac gactggatga ccggcagcaa acgcgacgac atcttcattt acaacgccgg
     1441 aatcaagaag ctgagggggc tcggcggaga cgatctcttc gtaaccaccg gccgcgggct
     1501 gggcaggata aatggcggcg gcggaacgga cacgatttcc ttcgctgcgc ttacttccag
     1561 caacgcctac
//
LOCUS       DAOQMF010000542         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_61538_length_1570_cov_3.742574, whole genome shotgun sequence.
ACCESSION   DAOQMF010000542 DAOQMF010000000
VERSION     DAOQMF010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61538_length_1570_cov_3.742574"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="PLV77_08755"
     CDS             <1..1148
                     /locus_tag="PLV77_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15978.1"
                     /translation="HDINDLDLVELKTLDFAYKWSPRDGSASVPFRGIATGSAQPPTG
                     FTANDFRIPTFDELLDAFPDTPINIELKQVGALTPADIQATAEKMADILEAHPGHDEN
                     VIINSFGQEMLDAMHAARPEHKSFSGSQSALLGYIQGNPITPTPVAVEPPDYYNLGSS
                     LQPTWVRTVPILSPFTEYDGYRIYVWGSDHDPFQDTDEFYAKLIEEGADAYNTPSPLT
                     LAAYLCSAGIPQADGTPRCPEQECPEGQTGYAPDHCVDIPVDPMIEIKSLKLTPKQGQ
                     VKAGKKAVLTLQVSTTDGIGGGNTPIRLRSSNRNVKVPASVKVYLVSGKTLTKKITVT
                     ATRKAKGKAAITATSGKFKANSVLKVKSLCVKKRSKGGNLCGKTNHF"
     gene            complement(1212..>1570)
                     /locus_tag="PLV77_08760"
     CDS             complement(1212..>1570)
                     /locus_tag="PLV77_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15979.1"
                     /translation="AFGRGLRVELTPLGASATVAYFGWIDTELVAGAFADPLVQGIRE
                     DVTPDWMTRKVPVSKAGKVVIDAIEKRNPRAIAPTEWKALFYARGLLVPAFDKRIDED
                     QAIAQIVPQAEERQRA"
BASE COUNT          320 a          534 c          457 g          259 t
ORIGIN      
        1 gccacgacat caacgatctc gatctcgtcg agctcaagac cctcgacttc gcctacaagt
       61 ggagtccgcg tgacggctcg gcctcggtgc ctttccgcgg catcgccacc ggcagcgccc
      121 agccgccgac cggtttcacc gccaacgact tcaggatccc gaccttcgac gagcttctcg
      181 acgctttccc ggacaccccg atcaacatcg aactgaaaca ggtcggcgcc ctgaccccgg
      241 ccgacatcca ggcgaccgcg gagaagatgg ccgacatcct cgaagcgcac cccggccacg
      301 acgaaaacgt gatcatcaac tccttcggcc aggagatgct cgacgcgatg catgcggccc
      361 ggcccgagca caagtcattc agcggcagcc agagcgccct gctcggctac atccagggca
      421 acccgatcac cccgactccg gtcgcggtcg agccgcctga ctattacaac ctcgggtcaa
      481 gccttcaacc cacatgggtg cgcacggtgc cgatcctgtc acctttcacc gaatatgacg
      541 gctacaggat ctacgtctgg ggttccgacc atgacccctt ccaggacacg gacgagttct
      601 acgccaagtt gatcgaggag ggcgccgacg cctacaacac gccctcgccg ctgactctcg
      661 ccgcctacct ctgctcggcc ggcatcccgc aggccgacgg cacccctcgc tgcccggagc
      721 aggaatgccc ggaaggccag accggctacg ccccggacca ctgcgtggat atccccgtcg
      781 atccgatgat cgagatcaag agcctgaaac tgactccgaa acaggggcag gtgaaggcag
      841 gcaagaaggc cgtgctgacg ctgcaagtct cgacaaccga cggcatcggt ggcggaaaca
      901 caccaatccg gttgaggtca tcgaaccgca acgtaaaggt gcctgcttcg gtcaaggtct
      961 acctcgtttc gggcaagacc ctgacgaaga agatcaccgt gaccgcaacc cgcaaggcga
     1021 agggcaaggc cgccattacg gcgaccagcg gcaaattcaa ggccaactcg gtgctcaagg
     1081 tcaagtccct ctgcgtgaag aagcgcagca agggcggcaa cctctgcggc aagaccaacc
     1141 acttttgaga atcagacagc gttgacgtta ttcgttccat gtgcggataa cttcaacgct
     1201 gtggtgagtt gtcaggcgcg ctggcgttcc tcggcctggg ggacgatttg ggcgatcgcc
     1261 tgatcctcgt cgatgcgctt gtcgaaggcg gggacgagca ggccgcgggc gtagaacagg
     1321 gccttccact cggtcggggc gatggctcgc ggattgcgtt tttcgatcgc gtcgatcacg
     1381 accttgccgg cctttgaaac cggcaccttg cgggtcatcc agtccggggt cacgtcttcc
     1441 cggatcccct ggaccagcgg atccgcgaat gctccggcca cgagctcggt gtcgatccag
     1501 ccgaaatagg cgacggtggc actggctccg agcggcgtca gttcgaccct cagcccgcgg
     1561 ccaaacgctt
//
LOCUS       DAOQMF010000543         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_61547_length_1570_cov_3.410561, whole genome shotgun sequence.
ACCESSION   DAOQMF010000543 DAOQMF010000000
VERSION     DAOQMF010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61547_length_1570_cov_3.410561"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLV77_08765"
     CDS             complement(<1..747)
                     /locus_tag="PLV77_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15980.1"
                     /translation="MRPPRKPTRRRGPDPAPEDTQEMFAAGQVVPGEPAGDEETREYE
                     TVATGEGETVDGKEAIEPVEEDEETVEALDEAETGVIEEDTEVIEEEHDTEVIEEEDD
                     TEVIEEEDDTEVIEEEAWDDEGYEDWEQEAEEVEEEPDPEAKAEPENSGGFAVWFTGF
                     RQRANEVWSGFWGRIGSIKLPRHELNGRKVLAVAGVLAIALMVGAGGYLLGKGSGDDV
                     DQARLEGEFAGRKAGAIAGALNQKYAAIQEA"
     gene            827..>1570
                     /locus_tag="PLV77_08770"
     CDS             827..>1570
                     /locus_tag="PLV77_08770"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC15981.1"
                     /translation="MGLCAGGVREGCRTVVAALAMLAFTVALAGPARANETVFGGELT
                     CVTQPGGVRQCAGPGGPDTNAPADTVPSFDGTPIDINFALPDKSRFGPPYPLAMYFHG
                     FGGEKEPFGGYLKRFTDRGIAALGMTERGFKESCGSEVAIEALDADTPGACDEGFIHL
                     MDSRYEVRDAQYFAGILADEGLIHPQKIGVVGGSYGGGKALALATLKNRVMLPDGSLV
                     PWKSPAGKSMAIAVAAPIVPWSDMAYALAP"
BASE COUNT          233 a          545 c          472 g          320 t
ORIGIN      
        1 ggcttcctga atcgccgcat acttctggtt gagcgccccg gcgatggcgc cggctttgcg
       61 gccggcgaac tcaccctcga gacgggcctg gtcgacatcg tcgccggaac ccttgccgag
      121 cagatagcca ccggcaccga ccatcagcgc gatcgccaga acccccgcca cggcgaggac
      181 cttccgtccg ttcagctcat gccggggcag tttgatcgaa ccgatccgac cccagaatcc
      241 ggaccagacc tcgttcgccc tctgcctgaa accggtgaac cagaccgcga aaccacctga
      301 attctccggt tcggccttcg cctccggatc cggttcctct tctacttcct ctgcttcctg
      361 ctcccagtct tcgtatccct cgtcgtccca ggcttcctct tcgattacct cggtgtcgtc
      421 ttcttcctcg attacctcgg tgtcgtcttc ttcttcgatt acctcggtgt cgtgttcttc
      481 ctcgattacc tcggtgtctt cttcgattac cccggtctcg gcctcatcga gagcttcgac
      541 ggtctcttcg tcttcttcga ccggctcgat ggcctctttc ccatcgaccg tttccccttc
      601 gcctgtcgcg acggtttcgt actccctggt ttcctcgtcc ccggcgggtt ccccgggcac
      661 gacctgccct gcggcgaaca tctcctgcgt gtcctcgggg gcagggtcgg gaccgcgtct
      721 tctggtgggc tttcttgggg gacgcatgga caaaatcctc agtcgggata cccaatgatt
      781 acccaagtac acctgaatgc ccttccctca ggtctagaat tgcggcatgg gcctgtgcgc
      841 gggaggagtg cgggagggat gccggacggt tgttgccgcc ctggcgatgc ttgctttcac
      901 ggtggcgctg gccggccccg cccgggccaa cgaaaccgtg ttcgggggag agctgacctg
      961 cgtgacccag cccggcggag tgcgccaatg cgccggtccc ggcgggccgg acaccaacgc
     1021 gccggctgac acggtcccga gctttgacgg gaccccgatc gacatcaact tcgccctgcc
     1081 cgacaagtcc aggttcggcc cgccctatcc gctggccatg tacttccacg gtttcggcgg
     1141 cgagaaggag cccttcggcg gctacctcaa acgattcacg gaccggggga tcgccgcact
     1201 cggcatgacc gaaaggggat tcaaggaatc ctgcggcagc gaggtcgcga tcgaagcgct
     1261 cgatgccgac acgcccgggg cctgcgacga gggcttcatc cacctgatgg acagccgcta
     1321 cgaagttcgc gatgcccagt acttcgccgg gattctggcc gacgagggcc tgatccatcc
     1381 gcagaagatc ggggtggtcg gcgggtccta cggcggaggc aaggcgctgg cgctggccac
     1441 gctgaagaac cgggtcatgt tgccggacgg cagcctcgtg ccgtggaaga gcccggccgg
     1501 caagtcgatg gcgatcgcgg tggccgcccc gatcgtgccc tggtccgaca tggcctatgc
     1561 cctggctccg
//
LOCUS       DAOQMF010000544         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_61719_length_1568_cov_2.547257, whole genome shotgun sequence.
ACCESSION   DAOQMF010000544 DAOQMF010000000
VERSION     DAOQMF010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61719_length_1568_cov_2.547257"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLV77_08775"
     CDS             <1..326
                     /locus_tag="PLV77_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15982.1"
                     /translation="QFFLAAMLAVALASTPSVARRMTPRWTNFGLAIVVLIPAAMLLF
                     MVPAIYSEIDNDNFLHASYHFGVLLLGFITGLGASMLGRVAGWAVLVSSIGMALMFAA
                     GVTGG"
     gene            329..793
                     /locus_tag="PLV77_08780"
     CDS             329..793
                     /locus_tag="PLV77_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC15983.1"
                     /translation="MEEEQQEQSGDGNGTGADEGGDRTLQAWLAGIAIGCLVVAAMIV
                     AYEIGKNNPDQEAAVKKPVAAAGSSGSPAPASPEASTFASTCGSCHTLAAADTDATVG
                     PNLDDLQPDSAMVMAAIENGGAGSGVMPAGLLSGEEAQAVADYVAKSAGSGK"
     gene            846..1544
                     /locus_tag="PLV77_08785"
     CDS             846..1544
                     /locus_tag="PLV77_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15984.1"
                     /translation="MRAMILGLAALLFLIPAAPARAHSNSPHLFESVPDQITPAHLAH
                     GIEVEMIDHDANLRLVNKSGNEVIVMGYDGEPYARLAANGEVFLNQRSPAYFLNQDRY
                     ATTPVEASADPEAPAKWVKQSDDGTLIWFDRRSHSMSDQVPAVVDDTSVSQPVRDYQI
                     PLRVNGEPAKLGGTLYWNGQSEFPMGIVAGLLIVTAMCAGLGFLGIEALRRAEPESHG
                     ASGGEVAPSPGPPQ"
BASE COUNT          277 a          525 c          495 g          271 t
ORIGIN      
        1 tccagttctt cctggccgcg atgctggcag tggcgctcgc ctcaacaccc tcggttgccc
       61 ggagaatgac gccccgctgg accaacttcg gcctcgccat cgtcgtgttg atcccggccg
      121 cgatgctcct tttcatggtt cccgcgattt attcggagat tgacaacgac aatttcctgc
      181 atgcctcgta tcactttggc gtcctgcttc tcggcttcat caccgggctc ggcgcttcga
      241 tgctcggccg tgtagcgggc tgggccgtac ttgtctcgtc gatcggaatg gcattgatgt
      301 tcgccgctgg cgtaactgga ggttgaagat ggaagaagaa cagcaagaac agtccgggga
      361 cggaaacggg accggcgcgg acgaaggggg cgaccggacg cttcaggcct ggctggccgg
      421 catcgcgatc ggctgcctgg tagttgcggc gatgatcgtc gcctacgaga tcggcaagaa
      481 caaccccgat caggaggccg cggtcaagaa gccggtcgcc gccgcagggt cctccggctc
      541 acctgccccg gccagtccgg aagccagcac cttcgcttcc acctgcggca gctgccacac
      601 cctcgcggcg gccgacaccg acgccacggt cgggcccaat ctggatgacc tccagcccga
      661 ctcggcgatg gtgatggcgg ccatagaaaa cggcggtgcc ggctccggcg tgatgccggc
      721 aggcctgctc tcgggtgagg aagcccaggc agtcgccgat tacgtggcca agtcggcagg
      781 ttccgggaag tagcgccaaa gtggaccggc atcgacgagc atcgaaagcc gggtccccga
      841 tcttcgtgcg agcgatgatc ctgggccttg ccgccctgct ctttctgatc ccggcggcgc
      901 cggcccgcgc ccacagcaat tcgcctcatc tgttcgaatc ggtcccggac cagatcaccc
      961 ccgcgcatct ggcccacggg atcgaagtcg aaatgatcga ccacgacgca aacctgcgcc
     1021 tggtcaacaa gagcggcaac gaagtgatcg tcatgggcta cgacggcgag ccgtacgccc
     1081 ggctcgcggc gaacggagag gtcttcctga accagcggtc acccgcctat ttcctcaacc
     1141 aggaccgtta cgcgacgacc ccggtcgaag cttccgcaga tcccgaagct ccggccaaat
     1201 gggtgaagca gagtgatgac gggaccctga tctggttcga ccggcgcagc cactcgatga
     1261 gcgaccaggt ccccgcggtg gtcgatgaca cttccgtctc gcagcccgta cgcgattacc
     1321 agatccccct gcgtgtcaac ggtgagccgg ccaagcttgg aggaaccctc tactggaacg
     1381 gccagagcga gttcccgatg gggatcgtgg ccggcctgct gatcgtcacg gcgatgtgcg
     1441 ccggtctcgg atttctgggc atcgaagccc tccgccgggc cgagccggaa agccatggcg
     1501 cctcaggggg cgaagtggcc cccagcccgg gcccacccca gtagcgcttc cttgttctcg
     1561 aacgggtc
//
LOCUS       DAOQMF010000545         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_61794_length_1567_cov_2.637566, whole genome shotgun sequence.
ACCESSION   DAOQMF010000545 DAOQMF010000000
VERSION     DAOQMF010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61794_length_1567_cov_2.637566"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLV77_08790"
     CDS             <1..206
                     /locus_tag="PLV77_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15985.1"
                     /translation="VNSDGFDGTPTADGAAVREALREQVQRRSREVASPPQGTGEAME
                     RIETFIRSLGDSAATVSNQNRRS"
     gene            209..1228
                     /locus_tag="PLV77_08795"
     CDS             209..1228
                     /locus_tag="PLV77_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HNC15986.1"
                     /translation="MRIGKILSAAAACAVLATGVAACGSDDNGGSSGDSKLKVAMVLP
                     GPINDKGFNQSGYDGLKQCEADGAEINYQEKTPVPDFEKAFETLAQSNDMVIGHGFEF
                     GEIAHKVAPDFPDTDFVVTSNPLEPEDSNVQHLMPNSTQGAYLAGVAAGKTTTSNKLG
                     GIAGFDFPVLEQQMKAVEAGAQSVNPKATLETVYLGTFDDVAKGKEAARSQAASGVDV
                     IYHIADAAGVGVIDGAEEEGIKAIGWGVDQSALAPKTIIASQLVDQAKMISDSCQEAI
                     DGKWKGGLVVDGLQSGLVDLTPVKNAPAGTQEAVDKARAAIIDGSAEVPSIGGTIPGS
                     GEGSN"
     gene            1302..>1567
                     /locus_tag="PLV77_08800"
     CDS             1302..>1567
                     /locus_tag="PLV77_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC15987.1"
                     /translation="MSALVQMRGIVKRFGDFTASREVDFDVAPGEVHALLGENGAGKT
                     TLMNILYGLVQADEGTIEVDGREVDLKGPADAIEAGVGMVHQHPL"
BASE COUNT          313 a          488 c          548 g          218 t
ORIGIN      
        1 tggttaactc cgatggcttc gacggcacgc cgacggccga cggcgcggcg gtcagggagg
       61 ccctgaggga gcaggttcag cgccgcagcc gcgaggtcgc ctcgccgccg caaggcacgg
      121 gcgaggcaat ggagcgaatc gagactttca tccggtcgct gggggactcg gcggccactg
      181 tgtccaacca aaacaggagg agttagaggt gcgtatagga aagattctca gcgctgccgc
      241 cgcgtgcgcg gtgctggcga cgggagtggc cgcttgcggt tccgatgaca acggaggctc
      301 atccggcgac tcgaagctca aggtcgcgat ggtgctgccc ggtccaatca acgacaaggg
      361 attcaaccag tccgggtacg acgggctgaa gcaatgcgaa gccgacgggg cggagatcaa
      421 ctaccaggag aagaccccgg tccccgactt cgagaaggcg ttcgagacgc tggcccagag
      481 caatgacatg gtgatcggcc acggcttcga gttcggcgag atcgcccaca aggtggcgcc
      541 cgacttcccg gacaccgact tcgtggtgac ctcgaacccg cttgaaccgg aggactccaa
      601 cgtccagcat ctgatgccga actcgaccca gggcgcgtac ctggccggcg tcgcggcagg
      661 caagacgacc acctcgaaca agctcggcgg gatcgccgga ttcgacttcc cggtgctcga
      721 gcagcagatg aaggcagttg aagcgggggc ccagtcggtc aacccgaagg ccacgctcga
      781 gaccgtctac ctaggcacct tcgacgacgt cgccaagggc aaggaagctg cccgcagcca
      841 ggccgcttcc ggagtcgacg tgatctacca catcgccgac gccgccggag tcggcgtgat
      901 cgacggcgcc gaagaggaag gcatcaaggc gatcggctgg ggtgtcgacc agagcgccct
      961 cgccccgaag accatcatcg cctcgcagct cgtcgatcag gcgaagatga tcagcgactc
     1021 ctgccaggag gcgatcgacg gcaagtggaa gggcggcctg gtggtcgacg gcctgcagtc
     1081 cggcctcgtc gacctgaccc cggtcaagaa cgctccggcc ggaacccagg aagcggtcga
     1141 caaggcccgc gcggccatca tcgacggctc cgccgaggtt ccgagcatcg gcggaacgat
     1201 cccgggttcg ggcgaaggct cgaactgagt tacgggttga acgcgtagcg aagacgagaa
     1261 gacccccggg ggcgaccgcc cccgggggag gaggttgttg agtgtcggca ctggttcaaa
     1321 tgcgggggat cgtcaagcgg ttcggtgatt tcaccgccag tcgcgaggtc gacttcgacg
     1381 tcgctcccgg agaggtccac gccctgctcg gagagaacgg agcgggcaag acgaccctga
     1441 tgaacatcct ctacggcctg gtgcaggccg acgaggggac gatcgaggtc gacggccgcg
     1501 aggtcgacct gaaggggccc gcggatgcga tcgaagcggg cgtcggcatg gttcaccagc
     1561 atcccct
//
LOCUS       DAOQMF010000546         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_62034_length_1564_cov_2.334659, whole genome shotgun sequence.
ACCESSION   DAOQMF010000546 DAOQMF010000000
VERSION     DAOQMF010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62034_length_1564_cov_2.334659"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLV77_08805"
     CDS             <1..262
                     /locus_tag="PLV77_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC15988.1"
                     /translation="DEPPAPSRKGLFGGSAPAATGGASDGVNLNTATFEELREAGLSV
                     TQATRILAYRERFGGYGSVEDLEKVPGFPADLIESLQGRITI"
     gene            complement(324..923)
                     /locus_tag="PLV77_08810"
     CDS             complement(324..923)
                     /locus_tag="PLV77_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC15989.1"
                     /translation="MKILAFSDLHRDLDQAARLTEMSRQADVVIGAGDFASIHQGLEE
                     TIDALAGIETPTVLVPGNNETLDALKEATAGWSAATVLHGEGIELDGKTFYGLGGGIP
                     VTPWDWSFDLEEADAGNMLAGLPAGAVLVVHSPPKGHCDESGNGMSLGSTAILKAIEE
                     KQPQLAVCGHIHEAWGKESKIGPTEVVNLGPSGRYFDLD"
     gene            complement(933..>1564)
                     /locus_tag="PLV77_08815"
     CDS             complement(933..>1564)
                     /locus_tag="PLV77_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HNC15990.1"
                     /translation="AGGKEYTAAELMVATGRRPRTDRIGLESIGVDGGAELETDDCYR
                     VKGHDWLFAVGDVNGRAPLTHMGKYQAWVGVRALLGHEPENRAELIGSPRVTFTNPQV
                     AAVGKTLAEAEAEGIDAVAIDADTGSTAGASFLGRGVDGKCRLVVDQATGLVVGATFV
                     GFEVAEWLQAATVAIVGKVSMDELRHAVAPFPTRSEIWLRFSEEWEARR"
BASE COUNT          237 a          550 c          511 g          266 t
ORIGIN      
        1 ggacgagccg ccggcccctt cacgcaaggg tctcttcggc ggttcggcgc cggccgccac
       61 cggcggcgcc tccgatgggg tcaacctgaa cacggcaacg ttcgaggagc tgagggaggc
      121 cggactttcg gtcacccagg cgacccggat cctcgcctat cgcgaacgct tcggcggtta
      181 cggctcggtc gaggatctcg agaaggtccc cggattcccg gccgacctga tcgagagcct
      241 tcagggccgg atcacgatct gacagggagt cgggcggggt ccgattcgcc ctccaggggt
      301 ggtcgggcag gcccggttcg aggtcagtcc aggtcgaagt accggccgga ggggcccagg
      361 ttgacgactt cggtcgggcc gatcttcgat tccttgcccc aggcttcgtg aatgtggccg
      421 cagacggcca gttgcggctg cttctcctcg atcgccttga ggatcgcggt cgaaccgagg
      481 ctcatgccgt tgccggattc gtcgcaatgg ccctttgggg gtgaatggac caccagcacg
      541 gcaccggccg gcaggccggc cagcatgttc ccggcgtcag cttcctcgag gtcgaagctc
      601 cagtcccagg gggtcaccgg gatgccgcca ccgagcccgt agaaggtctt gccgtcgagc
      661 tcgatcccct cgccatggag aacggtcgcc gcggaccagc cggcggtggc ttccttcagg
      721 gcatcgagcg tctcgttgtt acccggaacc agcacggtcg gggtctcgat cccggcgagg
      781 gcatcgatcg tctcctccag gccctgatgg atcgaggcaa agtcgccggc gccgatcacc
      841 acgtcggcct gccgggacat ctcggtcaac cgggccgcct ggtccaggtc acggtgcagg
      901 tcactgaaag cgaggatctt catgtgccaa ccttaccgcc gtgcttccca ctcctcgctg
      961 aacctcaacc agatttcgct tcgggtgggg aagggagcga cggcgtgccg cagttcgtcc
     1021 attgaaacct tgccgacgat cgcgacggtc gccgcctgca gccactcggc aacctcgaat
     1081 ccgacgaagg tggctccgac caccagtccg gtcgcctggt cgaccaccag ccggcacttg
     1141 ccgtcgactc ccctgccgag gaacgaggcc ccggccgtcg agccggtatc ggcatcgatc
     1201 gccaccgcat cgattccctc cgcctcggcc tcggcgaggg tcttgccgac cgccgccacc
     1261 tggggattgg tgaaggtcac ccggggagag ccgatcaact cggcccggtt ttccggctcg
     1321 tgcccgagca gggcccggac cccgacccag gcctgatact tgcccatgtg ggtgagcggc
     1381 gcccggccgt tcacgtcacc gacggcaaac agccagtcat gccccttcac ccggtaacag
     1441 tcgtcggttt ccagctctgc gccgccgtcg acgccgatcg attccagacc gatccggtcg
     1501 gtccggggcc ggcggccggt cgcgaccatt agttcggccg ccgtgtactc cttgcccccg
     1561 gcgg
//
LOCUS       DAOQMF010000547         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_62091_length_1563_cov_3.084881, whole genome shotgun sequence.
ACCESSION   DAOQMF010000547 DAOQMF010000000
VERSION     DAOQMF010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62091_length_1563_cov_3.084881"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLV77_08820"
     CDS             complement(<1..199)
                     /locus_tag="PLV77_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15991.1"
                     /translation="MWLLPDDRVRARVERRQYRSVVRHIFVSRRSGWISLREDGEAAG
                     AVLWYPPGTKKHSFRESLAESI"
     gene            complement(300..590)
                     /locus_tag="PLV77_08825"
     CDS             complement(300..590)
                     /locus_tag="PLV77_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase domain-containing
                     protein"
                     /protein_id="HNC15992.1"
                     /translation="MKLYTCGTGTSGPAGVHPCAKAGKALDDAGYEYELRKVRGYRML
                     PWTWKSRAEDRKEVKELSGTNEVPILVLDDGEVISDSSAIAKWAKENPAPGR"
     gene            complement(652..>1563)
                     /gene="purB"
                     /locus_tag="PLV77_08830"
     CDS             complement(652..>1563)
                     /gene="purB"
                     /locus_tag="PLV77_08830"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNC15993.1"
                     /translation="IDRAFEFKDTLAMGRTHGVHAEPTTFGLRLAGFAFEADRNYRRL
                     QRATEQAARGKLSGAVGTYASVPPAIERRVMDALGLTAEEIATQVVPRDRHAELLSAI
                     ALAGAGIERFATEVRHLQRTEVREAEEPFATGQQKGSSAMPHKRNPIRTERMTGLARL
                     LRGYAQTGLENVALWHERDISHSGAERVILPDATIGLDYMHHVITQVASGLVVHVDRL
                     QANLEQTNGAMFSQRALTALVESGMTRDDAYRTVQAAAHEAFDGDTHFRELIGKAAPE
                     LDLDEIFDYNAYLTHLPEVFERLEEIR"
BASE COUNT          218 a          541 c          526 g          278 t
ORIGIN      
        1 gaatgctctc ggccaggctc tccctgaagc tgtgcttctt ggttcccggc gggtaccaga
       61 gcaccgctcc cgccgcttcc ccgtcttcgc gaagggaaat ccagcccgac cggcgcgaaa
      121 cgaagatgtg cctgaccacc gaccggtact gccgccgttc gacccgggcc cggacccggt
      181 catcgggcag cagccacatc cagacttcgt tgtcctgaaa cccgcgagcg atcgcatcgg
      241 ccgcggccct ggcctcggac gggccgatca ggtctacacc gtggccagcc cctggccgct
      301 tacctgccgg gggcagggtt ctccttcgcc cacttggcga tcgccgacga gtcgctgatc
      361 acttcgccgt cgtcgagcac caggatcggc acttcgttgg tgccggagag ctcctttacc
      421 tccttgcggt cctcggcccg gctcttccag gtccagggca acatccggta gccgcgaacc
      481 ttcctgagtt cgtactcgta tccggcgtcg tcgagcgcct tgcccgcctt tgcgcaggga
      541 tgcacccccg ccggaccact ggtgcctgta ccgcaggtgt agagcttcat gcctggaggc
      601 taaacccaga aggcgaggcg tggtgcatac ccccgtgccg ggccctgctg cctagcggat
      661 ctcttcgagc ctttcgaaga cttcggggag gtgggtgagg taggcgttgt agtcgaagat
      721 ctcgtcgagg tccagctccg gggcggcctt gccgatcagc tcgcggaaat gggtgtcccc
      781 gtcgaaggct tcgtgggcgg cggcctggac cgtgcggtag gcatcgtcgc gggtcatgcc
      841 cgattcgacc agggcggtca gggcccgctg gctgaacatc gcgccgttgg tctgctcgag
      901 gttcgcctga agccggtcga catggacgac cagcccggaa gcgacctggg tgatcacgtg
      961 atgcatgtag tcgaggccga tcgttgcgtc gggcaggatc acccgctcgg ccccggagtg
     1021 gctgatgtcc cgctcatgcc agagcgccac gttctccagg ccggtctggg cgtagccgcg
     1081 cagcaaccgg gccaggccgg tcatccgctc ggtccggatc gggttgcgct tgtgcggcat
     1141 cgccgacgag cctttctgct ggccggtcgc gaacggctcc tcggcctcgc gcacctcggt
     1201 ccgctgcagg tgccggacct cggtcgcgaa ccgttcgatc ccggcaccgg ccagggcgat
     1261 ggccgaaagc agctcggcgt ggcggtcgcg cggcaccacc tgggtcgcga tttcctcggc
     1321 ggtaagtccc agcgcgtcca tcacccggcg ctcgatcgcc ggcggcaccg aggcataggt
     1381 gccgaccgcg cccgagagtt tgccccgggc ggcctgttcg gtcgcccgct ggagccgcct
     1441 gtaattgcgg tccgcctcga aggcgaaacc ggccaggcga aggccgaagg tggtcggctc
     1501 ggcgtggact ccgtgggtgc gccccatcgc cagcgtgtcc ttgaactcga aggcccggtc
     1561 gat
//
LOCUS       DAOQMF010000548         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_62531_length_1558_cov_2.239521, whole genome shotgun sequence.
ACCESSION   DAOQMF010000548 DAOQMF010000000
VERSION     DAOQMF010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62531_length_1558_cov_2.239521"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1157)
                     /locus_tag="PLV77_08835"
     CDS             complement(96..1157)
                     /locus_tag="PLV77_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNC15994.1"
                     /translation="MTDPALRGFASDNSSGAHPAMLEAIARVNGGHVGSYGADPETAR
                     FRELVKAEFGESAAGFPMLNGTGANVAALRAMARPHQAVICSSTAHIHIDETAAPEAI
                     AGLKLLTVDTADGKLTPELAATRLADPADVPHAAWPVVISVAQATEVGTVYTPDEIRA
                     LAEFAHERGMLLHIDGARLANAAASQDLSLNEASLGAGADVLSLGANKNGAVFGESVI
                     FADPELARGFEVLRKGSLQLASKMRFVSAQLCAQLEDGLWREIAGHSNSMARLLADGI
                     EWTEGADLGQPVQANIVFLSLPIEPARKLVEACTPHQPLLFEAGESGIIRLVCSWDTE
                     PGDVDQFLGLLRDVLPAPS"
     gene            1296..1377
                     /locus_tag="PLV77_08840"
     tRNA            1296..1377
                     /locus_tag="PLV77_08840"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1330..1332,aa:Tyr,seq:gta)
BASE COUNT          269 a          527 c          506 g          256 t
ORIGIN      
        1 gaaacctgct cgactgatcg ggtctacgga tctcatcgag attccacttt gcgccacata
       61 ccggcacaaa gtgggcactc ggcgctagct cggcgctagc tcggcgcggg aagaacgtcc
      121 cgcagaagac cgaggaactg gtccacgtcc ccgggctcgg tgtcccagga acagaccaga
      181 cgaatgatcc ccgactcgcc cgcctcgaag agcagcggct gatgcggtgt gcaggcctcg
      241 accagcttcc gggccggctc gatcggcagc gagagaaaga cgatgttggc ctgaaccggc
      301 tgaccgaggt cggcgccttc ggtccattcg atcccgtcgg caagcagccg tgccatcgag
      361 ttcgagtgac cggcgatttc ccgccagaga ccgtcctcga gctgggcgca tagctgggcc
      421 gagacgaagc gcatcttcga ggcaagctga agcgagccct tacggagcac ttcgaagcct
      481 cgagccagtt cagggtcggc gaagatcacc gactcgccga aaaccgcccc gttcttgttc
      541 gcccccaggc tcagcacatc cgcccccgcc ccgaggctgg cttcgttcag cgagaggtcc
      601 tgcgacgcgg cggcattggc cagccgtgcc ccgtcgatgt ggagcagcat gccccgttcg
      661 tgggcgaatt cggccagggc ccggatctcg tcaggggtgt agacggtgcc gacctcggtg
      721 gcctgggcga ccgagatcac gaccggccag gcggcgtggg gcacgtcggc cgggtcagcc
      781 aggcgggtcg cggccagctc cggggtcagc ttgccatcgg cggtgtccac ggtcagcagc
      841 ttgagtccgg cgatcgcctc gggcgccgcg gtctcgtcga tgtggatgtg ggcggtcgag
      901 gagcagatca ccgcctgatg cggccgggcc atcgcccgga gcgcggcgac gttcgccccg
      961 gtcccgttca gcatcggaaa gccggccgcg ctttcgccga actcggcctt caccagttcc
     1021 ctgaatcttg cggtctccgg atctgccccg taggagccca catgcccgcc gtttacccgg
     1081 gcgatcgcct caagcatcgc cgggtgggca ccggagctgt tgtcggaggc aaacccccgc
     1141 aaggcaggat cagtcatgcc tcgaagtatg cccggatgca gtgggtcgga taacccgcag
     1201 ctccgctgga aatccgaccc accccgtgag gccggcggtt cgggccgccc gcgagccacc
     1261 gaaatggccg ggtcaataaa ctgaccggct cagccggggg agtgcccgag tggttaaagg
     1321 ggactggctg taaaccagtt ggctctgcct acacaggttc gaatcctgtc tcccccatcc
     1381 agccggccgc ccaccgcggc cggctttttt ggtcaggccc cggccgggcg ctagtctgaa
     1441 taaaggacac gaggaacagg gagtcaactt gcgtctgttt caccgccaga tatcgatact
     1501 cgtcgcaatc gcgaccgtct tctgcttcgc gctgaccacc accgccttcg ccgaagac
//
LOCUS       DAOQMF010000549         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_62707_length_1556_cov_2.013991, whole genome shotgun sequence.
ACCESSION   DAOQMF010000549 DAOQMF010000000
VERSION     DAOQMF010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62707_length_1556_cov_2.013991"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /gene="glmS"
                     /locus_tag="PLV77_08845"
     CDS             complement(<1..1278)
                     /gene="glmS"
                     /locus_tag="PLV77_08845"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNC15995.1"
                     /translation="MCGIIGYVGNRPAEEILVAGLQKLEYRGYDSAGVAILAPEEGVK
                     KVRAVGNLDGLRSALQQTDLSSTDEADAEGHFTGIAHTRWATHGRVTEANAHPHGDES
                     GNVQIVLNGIVENYVKLKSRLQEDGVTFTSETDAEVVAHLIHRYYEGDLTEAVRKAYA
                     DLRGHYSIVALHSEAPGVLVGARKETPLIVGVGEHENFIASAIPAFLNETRLALTIDD
                     GEIVTLTADGVTVTKADGEPVERATEEVEWDEETAEKAGYDTFMMKEIHEQPEAIAET
                     ILDRLTEDDSVDLSDIGFGDEVFRQCSRIVIVACGTSYHAGLVGRYAIERWARVPVEM
                     DIASEYRYRDPVIGENDLVIGITQSGETADTLAAMRLARDRGAKVLAITNIMGSQATR
                     DSDAVLFTRAGLEIGVAATKTFVAQVAAMYLVAL"
     gene            complement(1341..>1556)
                     /locus_tag="PLV77_08850"
     CDS             complement(1341..>1556)
                     /locus_tag="PLV77_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNC15996.1"
                     /translation="VEVADREAIAGAEPVWTEVDRLNAELEGKGRVLLRPSGTEPLVR
                     VMAEAPSQEQAQEICDSLAGLVRSELG"
BASE COUNT          258 a          510 c          502 g          286 t
ORIGIN      
        1 aagggcgacc agatacatcg cggccacctg ggcgacgaag gtcttggtcg cggcgacccc
       61 gatctccagt ccggctcggg tgaagaggac cgcgtcggaa tcgcgggtgg cctggctgcc
      121 catgatgttg gtgatcgcca gaaccttggc cccgcggtcc cgggccagcc gcatcgcggc
      181 cagggtgtca gcggtctcgc ccgactgggt gatgccgatg acaaggtcgt tctcgccgat
      241 caccgggtcg cggtaacggt actcggaggc gatgtccatc tcgaccggca cccgggccca
      301 gcgctcgatc gcgtaacgac cgaccagacc ggcgtggtag ctggtgccgc aggcgacgat
      361 cacgatccgc gagcactggc ggaagacctc gtcgccgaaa ccgatatcgg aaagatccac
      421 actgtcgtct tcggtcagac ggtcaagtat ggtctcagca atcgcttccg gctgctcgtg
      481 gatctccttc atcatgaagg tgtcgtatcc ggccttctcg gcggtctcct catcccactc
      541 gacctcttcg gtagcccgct cgaccggctc gccatcggcc ttggtcacgg tgacgccgtc
      601 agcggtcagg gtgacgatct cgccatcatc gatggtcagg gcgaggcggg tttcgttgag
      661 gaaggcgggg atcgcggatg cgatgaaatt ttcgtgctcg ccgactccga cgatcagcgg
      721 cgtctccttg cgagccccga ccagcacgcc gggagcttct gaatggagcg cgacgatcga
      781 gtagtggccg cgcagatcgg cgtaggcctt gcggaccgct tcggtcaggt caccctcgta
      841 gtagcggtga atcagatggg cgactacttc ggcgtcggtt tcggaggtga aggtgacccc
      901 atcctcctgc aggcgggact tcagcttcac gtaattctcg acgatcccgt tgaggacgat
      961 ctggacattg ccggactcgt caccatgggg atgggcattg gcctcggtca cccggccgtg
     1021 ggtcgcccag cgagtgtggg cgatgccggt gaaatgacct tcggcgtcgg cttcatccgt
     1081 cgaagacagg tcggtttgct gaagagcgct ccgcagcccg tcgaggtttc cgaccgcccg
     1141 gaccttcttc acaccctcct cgggagcgag aatcgccacc cctgcagagt cgtacccacg
     1201 gtactccagc ttttggagac ccgccacgag gatctcctca gcgggccggt tgccgacata
     1261 tccgatgatt ccgcacatga gcgctaaagc ctagccatgc ggtcgcgccg ccggggagag
     1321 atcggtcacg gagggagaaa tcagccgagt tccgagcgga ccagcccggc cagcgagtca
     1381 cagatctcct gtgcctgctc ctggctggga gcctccgcca tgacccggac cagcggctcg
     1441 gtgccggaag gcctcagcag cacccggccc ttgccttcga gttcggcgtt cagccggtcg
     1501 acctcggtcc agaccggctc tgccccggcg atcgcctcgc ggtctgccac ctcgac
//
LOCUS       DAOQMF010000550         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_63320_length_1547_cov_3.674933, whole genome shotgun sequence.
ACCESSION   DAOQMF010000550 DAOQMF010000000
VERSION     DAOQMF010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63320_length_1547_cov_3.674933"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLV77_08855"
     CDS             <1..195
                     /locus_tag="PLV77_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC15997.1"
                     /translation="ESKGTDIFEFRDGKVCREHSFYDVVGNLRSLGLWPGIGSRTEAA
                     AMTAAGAAIRGTGMVRNLRP"
     gene            353..>1547
                     /locus_tag="PLV77_08860"
     CDS             353..>1547
                     /locus_tag="PLV77_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HNC15998.1"
                     /translation="MTEIDRGRLAELTQSELDRFVEENPKSKELFERARKSLVGGVPM
                     PWMMRWAGGFPVFAKLAIGARIIDVDDHTYIDFCLGDTGAMPGHDPEPVVRAITDQER
                     KGITTMLPTEDAPWVGEELTRRFGLDRWMFTLTATDANRAALRICRQITGKPKVLVFS
                     YSYHGSVDEAFAVAGPEGVTVSREGNVGAPVDPAQTTFAVEFNDLEALEAALATGEVA
                     CVLAEPAMTNMGIVLPDEGYHEALRNLTRKHGALLIIDETHTFSAGPGGCTAAWGLEP
                     DLLSIGKAIGSGVPAGALGMTEAVTESLFAQQDADYEDTGGVGGTLAGNALSLAAVRA
                     TLSEVLTEEAFAHTVPLATRFSEGVMQVVTDAGLPWNVTQLGARAEYRFEPEPARNGT
                     QAHNAS"
BASE COUNT          259 a          535 c          524 g          229 t
ORIGIN      
        1 gaatcgaagg gcaccgacat cttcgaattc cgggacggca aggtttgccg cgagcattcc
       61 ttctacgacg tggtcggcaa tctccggtcc ctcggcctct ggcccggcat cggcagccgg
      121 accgaagccg cggcaatgac cgcggcgggg gctgcgatcc gcggcaccgg catggtccgg
      181 aacctccgcc cttgaccccg cggcaccggg ctggtccgac acctccgccc ttgagacccc
      241 gacaccgcga atgcccactt tgcgccacat accggcacaa agtgggcact cggtgacccc
      301 ggcccccggc tagcggggcg catatgatcg gcccgacccc cgaggaggag aaatgaccga
      361 gatcgatcgt ggccgcctgg ccgagctcac ccagtccgag ctcgaccgtt tcgtcgagga
      421 aaacccgaag tcgaaggagc ttttcgagcg ggccaggaag tctctggtcg gtggcgtccc
      481 gatgccctgg atgatgcgct gggctggcgg tttcccggtc ttcgcgaagc tggcgatcgg
      541 ggcgcggatc atcgatgtgg acgaccacac ctacatcgac ttctgcctcg gagacacggg
      601 tgcgatgccc ggccatgatc ccgagccggt ggtgcgggcg atcaccgatc aggagcgcaa
      661 gggcatcacg acgatgctgc cgaccgagga cgcgccctgg gtcggggagg aactgacccg
      721 ccgcttcggt ctcgaccgct ggatgttcac cctgaccgcg accgatgcca accgggcggc
      781 gctgcgaatc tgccggcaga tcaccggcaa gcccaaggtt ctggtcttca gctacagcta
      841 ccacggctcg gtcgatgagg ccttcgccgt ggccgggccg gaaggcgtca cggtttcgcg
      901 ggaaggcaac gtcggagccc cggtcgaccc ggcgcaaacg actttcgcgg tcgagttcaa
      961 tgatctggag gcgctggagg ccgccctggc gacgggcgag gtcgcctgcg tgctggctga
     1021 accggcgatg accaacatgg gaatcgtcct gccggacgag gggtaccacg aggcgctgag
     1081 gaacctgacc cgaaagcacg gcgccctgct gatcatcgac gagacccaca ccttctcggc
     1141 cggcccgggc ggctgcaccg cggcctgggg cctggaaccg gacctgctct cgatcggcaa
     1201 ggcgatcggt tccggcgtgc cggccggggc cctcggcatg accgaagcgg tgaccgaaag
     1261 cctctttgcc cagcaggacg ccgattacga ggacaccggc ggggtcggcg gcaccctggc
     1321 cggcaatgcg ctctcgcttg ccgcggtccg ggcgaccctc tcggaggtgc tgaccgaaga
     1381 ggccttcgcc cacacggttc ccctcgccac ccgcttcagc gaaggtgtga tgcaggtggt
     1441 caccgacgcc gggttgccct ggaacgtgac ccagctcggg gcccgggccg agtaccgctt
     1501 tgagccggaa ccggcccgca acggcaccca ggcccacaac gcttccg
//
LOCUS       DAOQMF010000551         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_63521_length_1545_cov_2.875168, whole genome shotgun sequence.
ACCESSION   DAOQMF010000551 DAOQMF010000000
VERSION     DAOQMF010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63521_length_1545_cov_2.875168"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLV77_08865"
     CDS             complement(<1..628)
                     /locus_tag="PLV77_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC15999.1"
                     /translation="MSEEATEETSRPKVIVVMPARQAAATLKATFDEIPRDAVDEVIL
                     VDDSSTDETVDLARQLEINVVWHPHQVGYGGNQKTCYLEALQRGADVVVMLHPDGQYE
                     PGLIPSMIEPIVADQADLVLGSRLLVEGWALSAGMPRWKFIANRFLTTIQNRIMGTRL
                     SEAHTGYRAYSRELLLTVPFLRNSLDFVFDSELLYQASYFGFRIREVPA"
     gene            complement(621..>1545)
                     /locus_tag="PLV77_08870"
     CDS             complement(621..>1545)
                     /locus_tag="PLV77_08870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC16000.1"
                     /translation="VTRLKVDWTGAAATECVWCGRPLDARARRLTGRTICGSCGTGTT
                     DPLPDPESLDAAYGDWYWPSSGKRFGLVGDRMLRRSRASMAARIDEVAPPGPVLDVGA
                     GEGTLIDALKARGREARGLERLPSREDIVDLSLPEVEQDDFAAIIFWHSLEHLPDSGP
                     VMQAAAERLRPGGVAFVAVPDLSSPQARFFGDRWIHLDLPRHLVHLTTASLTAGMEAN
                     GLEVTATSRTRGGQNLIGWLDGFVAMLPGGLNLYQALRRKQARRIPLNPVQRLLSITA
                     GVLLAPVALVCAAVEVACRRSGTIYVEGRRV"
BASE COUNT          247 a          503 c          568 g          227 t
ORIGIN      
        1 gggccggaac ctcgcggatg cggaacccga agtaggaggc ctgataaagc agctcggaat
       61 cgaacacgaa gtcaagcgag ttgcgcagga acggaaccgt gagcagcagc tcgcgggagt
      121 aggcgcggta accggtgtgg gcctcggaga ggcgggtgcc catgatccgg ttctggatcg
      181 tggtgaggaa gcggttggcg atgaacttcc agcgcggcat gccggcggaa agggcccagc
      241 cctcgacgag caggcgcgaa ccgagcacga ggtcggcctg gtcggcgacg atcggttcga
      301 tcatgctggg gatgagcccc ggttcgtact ggccgtcggg gtggagcatc acgaccacgt
      361 cggcgccacg ctgcagcgcc tcgaggtagc aggtcttctg gttgcctccg tagccgacct
      421 gatggggatg ccagaccacg ttgatctcga gctggcgggc caggtcgacg gtctcgtcgg
      481 tcgaggagtc atcgaccagg atcacctcgt cgaccgcgtc ccggggaatc tcgtcgaagg
      541 tggccttcag ggtggcggcg gcctgccggg ccggcatgac cacgatgacc ttcggacggc
      601 tggtttcctc ggttgcttct tcagacacgg cgaccctcga cgtagatggt tcccgatcgc
      661 cggcaggcga cctcgaccgc ggcgcagact agcgccaccg gggccaggag cacgccggcg
      721 gtgatcgaaa gcaggcgctg gaccggattg agggggatcc gcctcgcctg tttgcggcgc
      781 agcgcctggt agaggttgag cccgcccggc agcatcgcga cgaagccgtc cagccagccg
      841 atcaggttct ggccgccgcg ggtgcgggag gtggcagtta cctcaagtcc gttcgcctcc
      901 atccccgcgg tcagcgaggc cgtggtcagg tggaccaggt gcctgggcag gtcgaggtgg
      961 atccagcggt cgccgaagaa ccgggcctgg gggctggaaa ggtcgggcac cgccacgaag
     1021 gcgaccccgc cgggcctgag ccgttcggcg gcggcctgca tcaccggccc cgagtcgggc
     1081 aggtgctcca gtgaatgcca gaagatgatc gccgcgaaat cgtcctgctc gacctccggc
     1141 agcgagaggt cgacgatgtc ctcgcgggag ggcagccgct caaggcctcg ggcttcccgg
     1201 ccccttgcct tgagggcgtc gatcagcgtg ccttcgccgg cgcccacgtc aaggaccggc
     1261 ccgggcggcg ccacctcatc gatccgggct gccatcgagg cgcgcgaccg gcgcagcatg
     1321 cggtccccga ccaggccaaa gcgtttgccc gaactgggcc agtaccagtc gccgtaggcg
     1381 gcatcgagcg attccgggtc cggcaagggg tcggtggtcc cggttccgca cgagccgcag
     1441 atcgtccggc cggtcagccg ccgggcccgg gcgtccagag gccggccgca ccagacgcat
     1501 tcggttgccg cggcgccggt ccagtccacc ttcagccggg tcacg
//
LOCUS       DAOQMF010000552         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_64273_length_1536_cov_2.204591, whole genome shotgun sequence.
ACCESSION   DAOQMF010000552 DAOQMF010000000
VERSION     DAOQMF010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64273_length_1536_cov_2.204591"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..388)
                     /locus_tag="PLV77_08875"
     CDS             complement(167..388)
                     /locus_tag="PLV77_08875"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC16001.1"
                     /translation="MSNRVSRRQANKWAAQDAQQAPQPQYVEPAAPAPAAAPAADPIA
                     QLKELGELHQSGVLTDEEFAAQKAKILGS"
     gene            complement(476..910)
                     /locus_tag="PLV77_08880"
     CDS             complement(476..910)
                     /locus_tag="PLV77_08880"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HNC16002.1"
                     /translation="MTEDIEEMGPIDYILVEWPNNEPTGEAIPYLVDLVDSGLIRILD
                     LAFIIKNEDGTVTEMEITDLGGDAETFAVFEGASSGILGESDHAEAAEALETGKAAAL
                     LVYENAWAGPFAAAVRRSGGQLVASGRIPVQDLLASLDAAEA"
     gene            complement(995..>1536)
                     /locus_tag="PLV77_08885"
     CDS             complement(995..>1536)
                     /locus_tag="PLV77_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNC16003.1"
                     /translation="RSGLRILGAMSLVYVVLALTNAELGRTEAAAADLETASKVGEGI
                     VDYIIWHEAELVLCRTRTLLRVGQFDQAEQELGRAEAAIERVPDSPRLSRWLEECRDE
                     LEKVRGSDGAPINLTKAELRTLQFLPSHHSFRSIGEQLYLSQNTVKTQANSLYRKLGV
                     NSRAEAVMEGRRLGLLKGE"
BASE COUNT          255 a          483 c          458 g          340 t
ORIGIN      
        1 atcgcccgga tgatcaacgc ctcgaactct tcgtccgaga ccgaatgggg atgctcgtga
       61 tgcttgtgct gaccgtgttt gccggtacca cgggtgaaga cggaggcccg cccgggagga
      121 cagactcccg gggcggactg ttcgtcatcg gtcaacgcct gccacctcaa ctgccgagga
      181 tctttgcctt ttgcgcggcg aattcctcgt cggtcagcac gccgctctgg tggagttcac
      241 cgagttcctt gagctgggcg atcgggtcag cagcgggggc cgctgccgga gctggagcag
      301 ccggttccac gtattggggc tgcggtgcct gttgcgcatc ctgcgcggcc catttgttgg
      361 cctggcggcg tgaaacacgg ttactcacgg ttgtcgccgt accggcgatg accgcggttc
      421 gggctacacc tctgatcaat cctggcattt tgttctcctt ctttctctga agttcctacg
      481 cttcggcagc gtcgaggctg gcgagcaggt cctggaccgg gatgcgtcca ctggcaacga
      541 gctggccgcc cgaacgacgc acggcggcgg cgaaggggcc agcccacgcg ttctcgtaaa
      601 cgagcagggc ggcggccttg cccgtctcaa gcgcttccgc agcttcggca tggtcgcttt
      661 caccgagaat ccccgacgat gctccttcga atacggcgaa ggtctcggca tcgccaccga
      721 ggtcggtgat ctccatctcg gtgaccgtgc cgtcctcgtt cttgatgatg aaagccaggt
      781 cgaggatccg gattagtccg ctgtcgacca gatcgaccag gtaggggatc gcttctccgg
      841 tcggttcgtt gttcggccac tcgaccagga tgtagtcgat cggccccatt tcttcgatgt
      901 cttcagtcaa tgctcggtcc ttcctcgatt tcaaactggg tttccacaaa atctagccac
      961 aagacgtttc cggccatcat ccattcgggg ttaatcactc gcccttgagc agtcccaggc
     1021 gccgaccctc catcactgcc tcggcccggg agttcactcc gagctttcgg tagagggaat
     1081 tggcctgcgt cttgacggtg ttctgcgaaa ggtagagctg ctctccaatc gaacggaaag
     1141 aatggtggct cggcagaaac tgcaaggttc tgagttcggc cttggtcagg ttgatcggcg
     1201 cgccgtcact tccccggacc ttctccagtt cgtccctgca ttcttcaagc caacggctca
     1261 gccgtggtga atcgggaacc cgctcgatcg ctgcctcagc ccggccgagt tcctgttcgg
     1321 cctgatcgaa ttgtcctacc cgcaacaggg tgcgagtgcg gcacagaaca agttcggctt
     1381 cgtgccagat gatgtagtca actatgcctt cgccgacttt cgacgcggtt tccaggtccg
     1441 cggcggccgc ttcggtccgg cccagttcgg cgttggtcag cgccagaacc acatatacaa
     1501 gggacatcgc acccaggatc ctgagcccgc tcctct
//
LOCUS       DAOQMF010000553         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_64772_length_1530_cov_2.989831, whole genome shotgun sequence.
ACCESSION   DAOQMF010000553 DAOQMF010000000
VERSION     DAOQMF010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64772_length_1530_cov_2.989831"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLV77_08890"
     CDS             complement(<1..236)
                     /locus_tag="PLV77_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC16004.1"
                     /translation="MGRILVVEDSSAIRLLIRRRLEMAGHQVEEAANGLEALDQLGST
                     AEPDLIVLDESMPVFDDAGLLTRLREQWSELPVIV"
     gene            complement(236..1303)
                     /locus_tag="PLV77_08895"
     CDS             complement(236..1303)
                     /locus_tag="PLV77_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC16005.1"
                     /translation="MAEFLTFGTHDYRIEDDLLFLRYRGLLTPDDAGRIIWANAPTSR
                     IAPLLLETRSIREALAGIAHEQKLDRLLIRHEVITCPGRTGGPDLHWMVWTEPLAGGE
                     WVLMVWEADWSEEMNERRTAFTMAASHELRTPLTALLGFAEILEMDTDNLKPAQIEAV
                     QMISQTALHLTKLVDDVFELTSNSFGELRLNLERVDLGEIARVLVEAHLQEAANRGDT
                     LTLEVQEDIPPVDADPARVRQMFANLVRNAMVHNPSGTRIVMKLWTDDDAVAVSVSDD
                     GRGLPFSNPKQALSSFSQGDGGSGDRTGAGIGLTIAKRLIELHRGSIEVQSTFGEGST
                     FTLRFPIDRAKAISPGEPGPA"
BASE COUNT          263 a          511 c          478 g          278 t
ORIGIN      
        1 acgatcaccg gcagctcgga ccactgttcc ctgagccggg tgagcaaacc ggcatcatcg
       61 aacaccggca tgctctcgtc aagcacgatc aggtccggct ctgcggtgga tccgagctga
      121 tcgagcgcct caaggccgtt cgcggcctct tcgacctgat gtccggccat ctccagtctt
      181 cgcctgatca gaagccggat cgccgatgaa tcctcgacca ccagaatccg gcccatcagg
      241 ccggtcccgg ctcgcccggc gaaatcgcct tcgctcgatc gatcgggaaa cgcagggtga
      301 aggtgctgcc ttcaccgaaa gtcgactgga cctcgatcga cccgcggtgc agttcgatca
      361 ggcgcttggc gatggtcagg ccaatcccgg cgccggtccg gtctccgctg ccgccgtctc
      421 cctggctgaa gcttgagaga gcctgtttcg ggttgctgaa ggggagcccc cggccatcat
      481 cggaaaccga gaccgctacc gcgtcgtcat cggtccacag tttcatcacg atcctggttc
      541 cggaggggtt atggaccatc gcgttacgca cgaggttggc gaacatctgt cggaccctcg
      601 ccgggtcggc gtcaaccggg ggaatatctt cctggacttc cagggtcagg gtgtcgcccc
      661 ggttcgcagc ttcctgcagg tgagcctcga ccagaacccg ggcgatctct ccgaggtcga
      721 cccgctccag gttgagcctg agttcaccga agctgttgct ggtcagttcg aagacgtcat
      781 cgaccagctt ggtcagatga agggcggtct gggaaatcat ctggaccgct tcgatctggg
      841 cgggcttcag gttgtcagta tccatctcga ggatttcggc gaaccccagc agagcggtca
      901 gcggcgtcct gagctcatgc gaggccgcca tggtgaacgc cgtgcgtcgc tcgttcatct
      961 cctctgacca gtcggcctcc cagaccatca ggacccactc gcccccggcc agtggctcgg
     1021 tccagaccat ccagtggagg tcgggtccac ccgtccggcc ggggcaggtg atcacttcat
     1081 gccggatcag caggcggtcg agcttctgct cgtgggcaat gcccgccagg gcttcccgaa
     1141 tcgaccgtgt ctccagcagg aggggtgcga ttcgtgaagt cggagcgttg gcccagatga
     1201 tccggccggc atcgtcgggc gtcagcaggc cgcgatagcg cagaaacagc agatcgtcct
     1261 cgatgcggta gtcgtgcgtg ccaaaggtca ggaactcagc catcgccccg atgcccccgc
     1321 gcccactata tcgcgttcgc ctccgtcggc gatctcgtcc ccgggcgaag aatttgcggg
     1381 tggcctgcct aggtcctcag acgggcgagg acagcatcta agagagtctg ctcagccgac
     1441 accgcggggg gcgacgcgga caagccggga ttcgccgccc cggcacatgt ccccgcacat
     1501 gtccccgcgc acgtcccggc gcaggcacac
//
LOCUS       DAOQMF010000554         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_65155_length_1525_cov_3.904762, whole genome shotgun sequence.
ACCESSION   DAOQMF010000554 DAOQMF010000000
VERSION     DAOQMF010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65155_length_1525_cov_3.904762"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..736)
                     /locus_tag="PLV77_08900"
     CDS             complement(203..736)
                     /locus_tag="PLV77_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNC16006.1"
                     /translation="MAKKKSFAKALNEQISNEFAASQQYVAMAVYYDSETLPRLAAFF
                     YRQALEEREHAMMMIQYLLDVDEEVEVPDVKSQPTKYEDGITPVKEALAQEERVSSEI
                     FGLFELAREIKDYRAEHFMQWFVKEQVEEVALMGDLLNVVERSKDNLLLAEEYIARES
                     FGEEGNDPTAPPPAGGN"
     gene            complement(832..>1525)
                     /locus_tag="PLV77_08905"
     CDS             complement(832..>1525)
                     /locus_tag="PLV77_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16007.1"
                     /translation="LDRPLNPLTFVASMGLAGGPGGTKSDVGRLRVKLDRYPDLKFKL
                     DPSLDWDDALIGELVETGAVNTLDLKGQYKGTVVDIETDPELYRRLVLAFPDAWIEDP
                     DLTPETREVLKDDWHRVTWDAPIHSVADIAELEHKPTVINIKPSRMGGLKSTLDAYDH
                     CEAEGIGAYGGGQWELGVGRDQIQYLAALFHPDNPNDTAPVGYNAAEPEAGLPVSPMK
                     VPGNGFGKPDQS"
BASE COUNT          251 a          490 c          476 g          308 t
ORIGIN      
        1 agacgaatgc agccaaagcg aatgcgaacg caagatggcg gcgcgtttct gcgaaacgaa
       61 tccccggaat catcaatttc tcctctcgcc aggcgcctct agcgccggac tccctattcc
      121 cgaatcggcg caggggccga ctcctcctgc ggtgaggata ccgcaggggg tgagggccta
      181 tctcacgcga tcagggtgaa gatcaattgc cgccggcagg aggcggggcg gtcgggtcgt
      241 tgccttcctc gccgaagctc tcgcgggcga tgtactcctc ggccagcagc aggttgtcct
      301 tcgagcgctc gaccacgttg agcaggtcgc ccatcagggc cacttcctcg acctgctcct
      361 tgacgaacca ctgcatgaag tgctcggccc ggtagtcctt gatttcgcgg gcgagctcga
      421 aaagtccgaa gatctcgctg gaaacccgct cctcctgggc cagcgcctcc ttgaccgggg
      481 tgatcccgtc ctcgtacttg gtcggctgcg acttgacgtc cggaacctcg acctcttcgt
      541 cgacgtcgag caggtactgg atcatcatca tcgcgtgctc acgctcttcc agggcctggc
      601 ggtagaagaa cgcagccagg cgcggcagcg tttcgctgtc gtagtagacg gccatcgcca
      661 catactgctg ggaggcggcg aattcattcg agatctgttc gttcagcgcc ttcgcgaaac
      721 tcttcttctt ggccatcttc tgctccgttc ttcgaggggt cgattcaagt cgccgacacc
      781 aacctagcgc agtccgcggc tttggatcgc cgtacgcgag gttagatgac tctaactctg
      841 atctggcttt ccgaagccgt ttccgggcac cttcatcggg ctgaccggca gcccggcctc
      901 gggttccgcc gcgttgtagc cgaccggtgc cgtgtcattc ggattgtccg ggtgaaagag
      961 cgccgccaga tactggatct ggtcgcgacc gacgcccagt tcccactggc cgccaccgta
     1021 ggcgccgatc ccttcggcct cgcaatggtc gtaggcgtcg agggtcgatt tcaatccacc
     1081 catccgggag ggcttgatgt tgatcacggt gggcttgtgt tcaagctccg cgatgtcggc
     1141 gaccgaatgg atcggcgcgt cccaggtcac ccggtgccag tcatccttca gcacctcgcg
     1201 ggtttcgggg gtcaggtccg ggtcctcgat ccaggcatcc gggaaagcca gaaccagccg
     1261 gcggtaaagc tccgggtcgg tttcgatgtc gaccaccgtg cccttgtact ggcccttgag
     1321 gtcgagcgtg ttgaccgctc cggtctcgac cagctcgccg atcagggcgt catcccagtc
     1381 gagtgacggg tcgagcttga acttgaggtc ggggtagcgg tcgagcttga cccgcagacg
     1441 gccgacgtcc gacttcgtgc cgccgggacc gccggccagc cccatcgagg cgacgaaggt
     1501 cagcgggttc agcgggcgat ccagg
//
LOCUS       DAOQMF010000555         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_66052_length_1515_cov_3.417123, whole genome shotgun sequence.
ACCESSION   DAOQMF010000555 DAOQMF010000000
VERSION     DAOQMF010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66052_length_1515_cov_3.417123"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1075)
                     /locus_tag="PLV77_08910"
     CDS             complement(5..1075)
                     /locus_tag="PLV77_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16008.1"
                     /translation="MSSPEVRIDQLTGGRVLVAPGRSERPDDFTPGAWHPAGHDGCPF
                     CEGNESKTPPEVEADRPEGGEANGPGWVTRTVPNLYPAVAPRESGEAADQRKVAGRAG
                     TESSAFSSPADPLLASGRGSEPDMFASRPAYGSHEVIVNSPFHLASLAELEEQSLAAA
                     VAAWRSRMRAHSDSAYVQLILNQGPDSGASLEHSHAQLGAMEFVPPSVARERERVGAY
                     RERTAGASLLGEILREEVRRGERLVAIDEEVALICPWASRHPYEMRLVPRRPVARFEE
                     DEGGAAMLGRAIRALTELFGNPCQLNLWVRTAPRGVEHFHWHLDLIPRLEPASAFEMA
                     TGVDINIVSPETAAETLRDAID"
     gene            complement(1083..>1515)
                     /locus_tag="PLV77_08915"
     CDS             complement(1083..>1515)
                     /locus_tag="PLV77_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC16009.1"
                     /translation="NESLQKIIDEQTEPWGVKVTTVEIKDVEIPEAMQRAMARQAEAE
                     RERRAKIINSEGEYQAAEKLTDAADIISRNPASLQLRYLQTLLEISGNQNSTVIFPLP
                     MDLISAFQQTLGNIGNADGGSDGGVQPTIGGARPDELSSEP"
BASE COUNT          220 a          503 c          533 g          259 t
ORIGIN      
        1 ctactcagtc gatggcgtcg cggagagtct cggcggcagt ttcgggactg acgatgttga
       61 tgtccacgcc agtagccatc tcgaaggcgg aggccggttc gagccggggg atcaggtcga
      121 ggtgccagtg gaagtgctcg accccgcggg gtgcggtgcg gacccagaga ttgagctggc
      181 aggggttgcc gaagagttcg gtcagcgccc ggatcgcccg gcccagcatc gccgccccgc
      241 cttcgtcttc ctcgaagcgg gcgacgggcc ggcgcgggac gagtcgcatc tcgtaggggt
      301 gacgggaggc ccacgggcag atcagggcga cctcttcatc gatcgcgacc agccgctcgc
      361 cacggcggac ttcctccctc aggatctcgc cgagcaggct ggccccggcg gtccgttccc
      421 ggtaggcccc gaccctttcg cgttcgcggg cgaccgatgg cggcacgaac tccatcgccc
      481 cgagctgggc gtgactgtgc tccagcgaag cgccggagtc ggggccctgg ttgagaatca
      541 gctgcacgta ggccgaatcc gaatgggccc gcatgcggct gcgccaggcg gcgaccgcgg
      601 cggccagcga ctgctcctcg agctcggcca gcgaggcgag gtgaaagggc gagttgacga
      661 tcacctcgtg gctgccgtag gcgggccgag aagcgaacat gtccggttcc gagccccgac
      721 cggaggcgag cagcggatcg gccggactgg agaaggcgct ggactcggtt ccggcccgac
      781 cggcgacctt gcgctggtcg gccgcttcac cggactcccg cggtgccacc gccgggtaaa
      841 ggttcgggac cgtgcgggtg acccagccgg ggccgttcgc ctcgcctcct tcaggccggt
      901 cggcttcaac ctcgggcggt gtcttcgatt cgtttccctc acagaacggg caaccgtcgt
      961 gaccggccgg gtgccacgcc cccggggtga agtcatccgg gcgctcggag cgacccggcg
     1021 cgaccagcac ccggcctcca gtcagctggt cgatcctgac ttcaggtgaa ctcaaagcgt
     1081 tttcagggtt ccgacgaaag ttcgtccggc ctggcgccac cgatcgtcgg ctgaacgcca
     1141 ccatcggagc cgccgtcggc gttgccgatg ttgccgagcg tctgctggaa ggcggagatc
     1201 agatccatcg gcaaggggaa gatcacggtc gagttctggt tgccggagat ctcgagcagg
     1261 gtctgcaggt agcgcagctg cagcgaagcc gggttgcggc tgatgatgtc ggccgcgtcg
     1321 gtgagcttct ccgccgcctg gtactcgcct tcggagttga tgatcttggc ccgccgttcc
     1381 cgttcggcct cggcctgccg ggccatcgcc cgctgcatcg cctcgggaat ctcgacgtcc
     1441 ttgatctcga ccgtcgtgac cttcacgccc cagggctcgg tctgctcgtc gatgatcttc
     1501 tgcagcgact cgttg
//
LOCUS       DAOQMF010000556         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_66148_length_1514_cov_2.887594, whole genome shotgun sequence.
ACCESSION   DAOQMF010000556 DAOQMF010000000
VERSION     DAOQMF010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66148_length_1514_cov_2.887594"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLV77_08920"
     CDS             complement(<1..781)
                     /locus_tag="PLV77_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNC16010.1"
                     /translation="MIELLLVLVSVALVVACGAFVAAEFSFVTVDRSRVDREADEGDR
                     RARGVRQGLRTLSTQLSAAQVGITVTNLLIGYLAQPSVASLIDAPLESLGVSAGAVDG
                     VALTVAFILANAVTMIFGELVPKNLAIANAMGTAKAVQGFQRGFTRIIHPLVRVTNGI
                     ANWILRRVGIEPQEELASARSPEELTSLVRRSAEKGTLEEPTAELLEKSLEFGELRAD
                     DVMTPRVKVKTLAPDQPVIAVIEAARAGGFSRFPVISEGSEI"
     gene            966..>1514
                     /locus_tag="PLV77_08925"
     CDS             966..>1514
                     /locus_tag="PLV77_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNC16011.1"
                     /translation="MASLLRRLLFAVLAATFLISSGASAAAPEPVVDGNQIVDSATGD
                     QFVPHGVNFPGYEYACKQGWNYGEEGTRPAEIASTVAGIASWNINTVRLPLNQDCWLG
                     DDGLPAGPGRTVAGYRQSVEDFVNALNAAGIVAIVDIHWSGPDGVVADGLRPMPDNRS
                     PAFWSSVATTFKDNPSVIFDLFN"
BASE COUNT          261 a          514 c          489 g          250 t
ORIGIN      
        1 cgatctcgga tccttcgctg atcaccggga aacgggaaaa gccaccggcc cgggccgcct
       61 cgattaccgc gatcaccggt tgatcgggag ccagggtctt gaccttgacc cgcggagtca
      121 tcacgtcgtc tgccctgagc tcgccgaact cgagtgactt ctcgagcaac tcggcggtcg
      181 gctcctccag cgtccccttt tcagccgagc ggcggaccag cgaggtcagc tcctccggtg
      241 accgggccga ggccagttct tcctggggct cgatcccaac ccggcggagg atccagttgg
      301 cgatgccgtt ggtcaccctg accagcgggt ggatgatccg cgtgaatccg cgctggaaac
      361 cctggaccgc cttggccgtc cccatcgcgt tggcgatcgc gaggttcttg ggcaccagct
      421 cgccgaagat catggtgacg gcgttggcga ggatgaaggc gaccgtcaaa gcgacaccgt
      481 caacggcgcc ggcagaaacc ccgagcgact ccagcggggc gtcgatcagc gaagcgaccg
      541 agggctgggc gaggtaaccg atgagcaggt tggtgaccgt gatcccgacc tgggcggccg
      601 aaagctgggt tgaaagggtc cgcagcccct gccggactcc gcgggcccgg cggtcacctt
      661 catcggcctc gcggtcaacc cgggaccggt ccacggtgac gaaggagaat tcggcagcga
      721 cgaaggctcc gcaggccacg acgagggcaa cggaaacgag tacgagcagc agctcgatca
      781 tcgaggcagc gctcccggca cccggctcag gaagcgcctg ggactagaac cggggtcgtc
      841 gtccatccag gtttccgcga gacttcatcc ggttcgaaga ttacccctca cgggcagatg
      901 catcggtccg atacccgctc cgatcccgct gatcgcgggt ttactacccc agataccctt
      961 tgtcagtggc atccctcctc agacgcctgc tttttgccgt gctcgcggcc acattcctga
     1021 tctcgtcggg ggcgtcggcg gctgcgccgg aaccggtggt tgacggcaac cagatcgtcg
     1081 attcggcgac cggggatcag ttcgtcccgc acggggtcaa ttttcccggc tacgagtacg
     1141 cctgcaagca gggctggaac tacggggagg aaggcacccg cccggccgag atcgcgtcga
     1201 ccgtggccgg gatcgcatct tggaacatca acaccgtccg gctcccgctg aaccaggact
     1261 gctggctcgg cgatgacggg ctgcccgccg ggccgggccg caccgtggcc ggataccgcc
     1321 agtcggtcga ggatttcgtc aacgccctca acgcggccgg catcgtcgcg atcgtcgaca
     1381 tccactggtc cgggccggac ggcgtcgtgg ccgacggcct gcgcccgatg cccgacaacc
     1441 gctcgcccgc cttctggagt tcggtcgcaa cgactttcaa ggacaacccg tcggtcatct
     1501 tcgacctctt caac
//
LOCUS       DAOQMF010000557         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_66334_length_1512_cov_2.575841, whole genome shotgun sequence.
ACCESSION   DAOQMF010000557 DAOQMF010000000
VERSION     DAOQMF010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66334_length_1512_cov_2.575841"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLV77_08930"
     CDS             complement(<1..936)
                     /locus_tag="PLV77_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNC16012.1"
                     /translation="MRDRQGGMLAGVCSGIALRLKVDPTLVRVVAVVLTVITGGVAAI
                     GYGLLWVALPVAGEEAIVRRWKPRQGSREKVAAGPAPARPFKGSWAVAAGAGFVTLGL
                     LFVFRETGVWWSDSLVWPLVIAAAGAALIWRQFSQSSRTILGPFRGGVGIALLLGGAV
                     LFLYINGALSAGGDVALAGLVAVIVIGLLAAPIWARSARQLSTERAERIRSEERAEVA
                     AHLHDSVLQTLALIQKNADDSRQVSTLARSQERELRDWLSGKTDRDRADSLAESLKAV
                     AAEVEERHQVPIDAVTVGDRPLDENSIALVAASREA"
     gene            complement(960..>1512)
                     /locus_tag="PLV77_08935"
     CDS             complement(960..>1512)
                     /locus_tag="PLV77_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16013.1"
                     /translation="SIADLRVEGSYGEIRETPLTIADIPGDGYKMAAGNMTIDLRKFH
                     FSPLHEQSIEVKSGMGLTSVIVPDDVCVSGLVTGKAGIANIRGLESNGLDIHQSAPGP
                     SDWKPGMRSRGNLLLIDGDFKLGAFEVVDATQWRRDGRGGGFEDNDLDPDARASTAAR
                     ARATAACAPRPTRLEKLKATASS"
BASE COUNT          235 a          513 c          529 g          235 t
ORIGIN      
        1 cgcctcgcga ctggcggcga ccagggcgat cgagttctcg tcgaggggcc ggtcaccgac
       61 ggtgaccgca tcgatcggca cctgatgcct ctcctcgact tcggcggcga ccgccttcag
      121 cgactcggcc agcgagtcgg cccggtcgcg gtcggtcttg cccgagagcc agtcgcgcag
      181 ttcccgctcc tgcgagcggg cgagggtcga gacctgacgg gagtcgtcgg cgttcttctg
      241 gatcagggca agggtctgga ggaccgaatc atgcaggtgg gcggcgactt cggccctttc
      301 ctcggagcgg atccgctcgg ctctctcggt gctgagctgg cgggccgagc gggcccagat
      361 cggggcggcc aggagaccga tcacgatcac cgcgaccagt ccggccaggg cgacgtcgcc
      421 accggcgctg agagccccgt ttatgtagag gaagaggacg gccccgccca gcagcagggc
      481 gatgccgacc ccgccccgga agggtccgag gatggtgcgg gatgactggc tgaactggcg
      541 ccagatgagc gccgccccgg cggcggcgat gaccagcggc cagaccaggg agtccgacca
      601 ccagaccccg gtctcacgga agacgaagag cagcccgagg gtgacgaaac cggccccggc
      661 ggcaaccgcc caggagcctt tgaagggtcg tgccggggcc ggtcctgcgg cgaccttctc
      721 ccggctgccc tgccggggtt tccagcgacg gacgatcgcc tcttcgccgg ccaccggaag
      781 ggcaacccag agcaggccgt agccgatcgc cgcgaccccg ccggtgatca cggtcaacac
      841 caccgcgaca acccggacca gggtcgggtc gaccttgagc cgcagcgcga tgccggagca
      901 gaccccggcc agcatcccgc cctggcggtc gcgcatgagc cgcttgtcgg gtggttcgtt
      961 caggagctgg ccgtcgcctt cagcttctcg aggcgcgtcg ggcgaggtgc gcaagctgcg
     1021 gtcgcccgtg cgcgtgcggc ggtcgatgcc cgcgcatcgg gatcgagatc gttgtcttcg
     1081 aagccgccgc cgcggccatc ccgacgccac tgggtcgcgt ccacgacctc gaaggctccg
     1141 agtttgaaat cgccgtcgat cagcagcagg ttgcctctgc ttcgcatgcc gggcttccag
     1201 tcggacgggc cgggagcgct ctggtggata tcgaggccgt tcgattcaag cccgcgaatg
     1261 ttggcgatcc cggccttccc ggtgaccagg ccgctcacgc agacgtcgtc cggaacgatg
     1321 accgaagtca ggcccatgcc gctcttcacc tctatcgact gttcgtgaag cggggaaaag
     1381 tgaaacttcc gcaagtcgat agtcatgttc ccggccgcca tcttgtagcc gtcgccgggg
     1441 atgtcggcaa tggtcagggg ggtttccctg atttcgccgt agctgccttc gacccggagg
     1501 tcggcgatcg ag
//
LOCUS       DAOQMF010000558         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_66732_length_1507_cov_3.808540, whole genome shotgun sequence.
ACCESSION   DAOQMF010000558 DAOQMF010000000
VERSION     DAOQMF010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66732_length_1507_cov_3.808540"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PLV77_08940"
     CDS             <1..1081
                     /locus_tag="PLV77_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC16014.1"
                     /translation="FLWVARMIMTGLEFAGDVPFHDVYVHSVIQARDGRRMSKSLGTG
                     IDPLEEIDEHGADALRFGLLAMSSTQDVRYSDPKVQQGRDLATKLWNASRLILLNTED
                     PADRTVPAAPICLEDRWILSRLETTIETYTRLIGEYDFAHAAQELYSFVFSDLCDWYL
                     EIAKPRIYDGDPEISQVLLHTLERTLALAHPVMPFVTEAIWEYHPYRQGHLVVHPFPV
                     PDSSRISEAVETEVGEAIGVTRQLRGWRDMAGVPPKVVLDAAGDPGSVPEFVPRLGRV
                     NIGAGEGETVATVAGFGILASDELDMEAVTARIGARRGKLEAELKKIEGKLANPRFVE
                     NAPAEVVAEEREKVGRLQSELAELG"
     gene            1089..>1507
                     /locus_tag="PLV77_08945"
     CDS             1089..>1507
                     /locus_tag="PLV77_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16015.1"
                     /translation="MTGPADEAPASPFDSEAFLNSLEPIGWKLGLERMEMLSDELGRP
                     QDSFATIHVVGTNGKSSVSRMIAAICEAHGFRAGCSLSPHLAHWSERVAISGEEQPGL
                     FADCVRRAAEAAASVNARLEGEEVTQFELATAAASSN"
BASE COUNT          247 a          518 c          490 g          252 t
ORIGIN      
        1 cttcctctgg gtcgcccgga tgatcatgac cggcctcgag ttcgccggcg acgttccctt
       61 ccatgacgtc tatgtccatt cggtgatcca ggctcgcgac ggccgccgca tgtcgaagag
      121 cctcggcacc ggcatcgacc cgctcgagga gatcgacgaa cacggggcgg acgccctgcg
      181 cttcggcctg ctcgcgatgt cctcgaccca ggatgtccgc tactcggacc cgaaggtcca
      241 gcagggccgc gacctggcga ccaaactctg gaacgcctcc cgcctgatcc tgctcaacac
      301 ggaggacccc gccgaccgca cggtcccggc cgcgccgatc tgcctggagg accgctggat
      361 cctttcccgc ctcgagacca cgatcgagac ctacacccgg ctgatcgggg aatacgactt
      421 cgcccatgcg gcccaggagc tctactcctt cgtcttctcc gatctctgcg actggtacct
      481 ggagatcgcc aagccgcgga tctacgacgg tgatccggag atcagccagg tcctgctcca
      541 caccctggag cgcaccctcg ccctggccca tccggtcatg cccttcgtga ccgaagcgat
      601 ctgggagtac cacccctacc ggcagggtca tctggtcgtt catcccttcc ccgtcccgga
      661 ttcctcgcgg atctccgagg cggtcgaaac cgaggtcggc gaggcgatcg gggtgacccg
      721 gcagctgcgt ggctggcgcg acatggccgg ggtgccgccg aaggtcgtgc tcgatgcggc
      781 cggtgatccg ggatcggttc cggaattcgt gccacggctg ggccgggtca acatcggcgc
      841 cggtgaaggc gagacggtcg caaccgtcgc cggcttcggc atcctcgcct cggatgaact
      901 cgacatggag gccgtgaccg cgaggatcgg cgcccgtcgc ggcaagctcg aagcggagct
      961 gaagaagatc gaaggcaagc tcgccaaccc gcgcttcgtt gaaaacgctc cggcggaagt
     1021 ggtcgcggaa gaacgcgaga aggtcgggcg gcttcagtcc gagctggccg aactcggctg
     1081 aggcgcccgt gaccggcccc gccgacgagg cccccgccag ccccttcgat tccgaagcgt
     1141 ttctcaactc gctggaaccg atcggctgga aactagggct ggagcggatg gagatgctct
     1201 ccgacgagct cggccgcccg caggactcgt tcgcaacgat tcacgtggtc ggcaccaacg
     1261 gcaagtcctc ggtttcgcgg atgatcgccg cgatctgcga ggcccatggc ttccgggcag
     1321 gttgctcgct ttcgccgcac ttggctcact ggtccgagcg ggtcgccatt tccggcgagg
     1381 aacagccggg gctgttcgcc gattgtgtgc ggcgtgcggc cgaagccgcg gcaagcgtga
     1441 acgcccgtct cgagggtgaa gaggtgaccc agtttgaact ggcgaccgcg gccgccagtt
     1501 caaactg
//
LOCUS       DAOQMF010000559         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_66845_length_1506_cov_2.305996, whole genome shotgun sequence.
ACCESSION   DAOQMF010000559 DAOQMF010000000
VERSION     DAOQMF010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66845_length_1506_cov_2.305996"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLV77_08950"
     CDS             complement(<1..268)
                     /locus_tag="PLV77_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HNC16016.1"
                     /translation="MVDTGDTAWMMMSTALVLMMTPALGLFYAGLVRSKNTLNTFMMS
                     VAALGVATICWVAVGYSIAFSGGEGGFIGNLDLAFLSGVGFEPRD"
     gene            complement(277..636)
                     /locus_tag="PLV77_08955"
     CDS             complement(277..636)
                     /locus_tag="PLV77_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNC16017.1"
                     /translation="MDMKLVIGIVRPEMANDVLEALFRAGVRGLSMSKVQGHGGELQR
                     VETYRGTTVQMGLSEKIRFEIAVSEEYVEKTVKALSEGGRTGEVGDGKIFVLSLDSVV
                     RVRTGETDDQAVTPVKS"
     gene            complement(723..1286)
                     /locus_tag="PLV77_08960"
     CDS             complement(723..1286)
                     /locus_tag="PLV77_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16018.1"
                     /translation="MAKKKLKSKSRNKKKRLTLTELHHEAVGKSVELLREKPGYKTLE
                     RQTVDFGDFEYPLDGVNSTGSRMVEINAHVGTMKSVDLPKVTEDILRFAAIRSQPGRE
                     KAKCEIYFVDQKARDSISGWIREAAAELGVDLLVVEGFPEKLHRKLTRAQQSRAKKTG
                     KKQALEDRLRKEIRNEIEIQYRLSQEA"
BASE COUNT          247 a          506 c          438 g          315 t
ORIGIN      
        1 cgtcacgagg ttcgaatccg actccgctca ggaaggccag gtcgaggttc ccgatgaaac
       61 ctccctcgcc cccgctgaag gcgatcgagt atccgaccgc gacccagcag atcgtggcga
      121 cgccgagcgc ggcgacgctc atcatgaagg tgttgagcgt gttcttcgac cgcaccagcc
      181 ccgcgtagaa gagaccaagt gccggggtca tcatcagcac cagggcggtg gacatcatca
      241 tccatgcggt atctccggta tcgaccatgg ttcccctcag gacttgaccg gcgtgacggc
      301 ctggtcgtcg gtttccccgg tccggacccg gaccaccgag tcgagcgaga ggacgaagat
      361 cttgccgtcc ccgacctccc cggtccgccc gccctcgctc agggccttca ccgtcttctc
      421 gacgtactcc tcggagaccg cgatctcgaa cctgatcttc tccgagaggc ccatctggac
      481 cgtcgtcccg cgataggtct cgacgcgctg cagctcaccc ccgtggccct gcaccttgct
      541 catcgagagc ccgcggactc ccgcgcgaaa cagcgcttcg agcacatcgt tggccatttc
      601 cggccgaacg atgccgatca caagcttcat atccatctcc cccggtcaaa gactggttgg
      661 cactggaaac cagcctagtc ccggcgtcgg acctcgccgc cggcatccgg cggcggggcg
      721 cttcaggctt cctgggagag gcggtactga atctcgatct cgttgcggat ctccttgcgg
      781 agacgatcct cgagagcctg cttcttgccg gtcttcttgg cccggctttg ctgggccctg
      841 gtcagcttcc ggtgaagctt ttcggggaac ccttcgacca ccaggaggtc gacaccgagt
      901 tcggcagcgg cttcccggat ccagccggaa atcgaatccc tggccttctg gtccacgaag
      961 tagatctcgc acttcgcctt ctcgcgaccg ggctgggacc tgatcgccgc aaaacggaga
     1021 atgtcctcgg tcaccttcgg caggtcgacg ctcttcatcg tgccgacgtg ggcgttgatt
     1081 tcgaccatcc tgctgccggt tgagttgacg ccgtccagcg gatactcgaa gtctccgaag
     1141 tcgaccgtct gccgctcgag ggtcttgtag ccaggcttct cccgaaggag ctccacggac
     1201 ttgccgaccg cctcgtggtg aagctcggtg agggtcagtc gcttcttctt gttcctgctc
     1261 tttgacttca gtttcttctt tgccatcttc gtacctcagt cgagagtgtg cctgacagga
     1321 cttccacagc ctagccccct tcatggtgtg ccggagcgcc gacctggtcc ctaaggtgaa
     1381 ggggatgaat gagggagagg aacggaagag ttccgcctgg ttcggcaccc tgggcgcggc
     1441 gatggagtgg tacgacttca cgctctacgt ctatctcgcg ccggtcatct cgcagctgtt
     1501 cttccc
//
LOCUS       DAOQMF010000560         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_67070_length_1503_cov_3.989641, whole genome shotgun sequence.
ACCESSION   DAOQMF010000560 DAOQMF010000000
VERSION     DAOQMF010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67070_length_1503_cov_3.989641"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PLV77_08965"
     CDS             <1..1233
                     /locus_tag="PLV77_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC16019.1"
                     /translation="GSYLGIHGVLEDAATNWFGLQGFEYLDLARLWQVLLVVGLIVWV
                     YMLFRVMRSRLRREHPGNMPWLFFLAACAIPAFYAVGLLARTAEDFTITEFWRFWVVH
                     LWVEDFLEIFTTVMVAYMFVLLGVVREKVALMVILLDIGLYSVGGVVGTMHHLYFSGT
                     PSEHMALGAFFSAIEVVPLTFLTVEAWSFLQLGANQESKSTSDFPHRWAVMFLVAVGF
                     WNFLGAGVFGFLVNLPIVSYFEIGTALTANHAHAAMMGVYGMLAIGLSLFCLRYMIPS
                     KKWPEKWAKVSFWGTNIGLAWMCFATLLPLGIMQLYKSVDDGYFEARQLDFLTDKTTE
                     MLEWMRLPGDVVFIVFGAIPVLYMCFLGVKYTIARRGEDGDGRGEADAELFTVISGTS
                     MEDEPPEISGRPEPTPGT"
     gene            1230..>1503
                     /locus_tag="PLV77_08970"
     CDS             1230..>1503
                     /locus_tag="PLV77_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16020.1"
                     /translation="MNTEVLLAVGYAALLLGGALLLEWLSRHTHNRSQAWRTSGFDYD
                     AQHDFWVCHKGEQLWPTEFDREKRLVRYRAKATVCNACPAKVDCTDS"
BASE COUNT          228 a          483 c          471 g          321 t
ORIGIN      
        1 ggttcctatc tggggatcca cggtgtgctg gaggatgccg cgaccaactg gttcgggctt
       61 cagggattcg agtatctcga cctcgcccgc ctctggcagg tgctgctggt ggtcggcctg
      121 atcgtctggg tctacatgct cttccgggtg atgcgctccc ggctccggcg tgagcatccc
      181 ggcaacatgc cctggctttt cttcctcgcc gcctgcgcaa tcccggcgtt ttacgcggtc
      241 ggcctcctgg cccggaccgc cgaggacttc acgatcaccg agttctggcg cttctgggtg
      301 gtccacctct gggtcgagga cttcctcgag atcttcacga cggtgatggt cgcctacatg
      361 ttcgtgctgc tcggggtggt gcgcgagaag gtggcgctga tggtgatcct gctggacatc
      421 ggcctctatt cggtcggcgg cgtggtaggc actatgcacc acctctactt ctccgggacc
      481 ccgtccgagc acatggcgct cggcgcgttc ttctcggcga tcgaggtcgt gccgctcacc
      541 ttcctcacgg tcgaggcctg gtcgttcctg cagctcggcg caaaccagga atcgaagtcg
      601 acctccgact tcccgcaccg ctgggcggtc atgttcctgg tcgcggtcgg tttctggaac
      661 ttcctcgggg ccggcgtctt cggcttcctg gtcaacctgc cgatcgtctc ctacttcgag
      721 atcggcaccg cccttaccgc caatcacgcc catgcggcga tgatgggcgt ctacggcatg
      781 ctggcgatcg gcctctccct gttctgtctc aggtacatga tcccctcgaa gaagtggccg
      841 gagaaatggg ccaaggtcag tttctggggt accaacatcg ggctcgcctg gatgtgcttt
      901 gcgaccctgc tgccgctggg gatcatgcag ctctacaagt cggtggacga cggctatttc
      961 gaggcccgcc agctcgactt cctgaccgac aagacgaccg agatgctcga gtggatgcgg
     1021 ctgcccggtg acgtggtatt catcgtcttc ggtgccattc ccgtgctcta catgtgtttc
     1081 ctcggagtca agtacacgat tgcccgtcgc ggcgaagacg gggacggccg gggtgaagcg
     1141 gacgccgaat tgttcacggt gatctccggc acttccatgg aagacgaacc gccggagatc
     1201 tccggccggc cggaaccgac cccgggaacg tgaacaccga ggtcctcctg gcagtcggat
     1261 acgcggccct tctgctggga ggggctctgc ttctcgagtg gctttcccgc cacactcaca
     1321 accggtcgca agcctggcgg acctccggct tcgattacga cgcccagcat gacttctggg
     1381 tctgccacaa aggggaacag ctctggccga ccgagttcga ccgggaaaag cggctggtca
     1441 ggtaccgggc caaggcgacg gtgtgcaatg cctgcccggc caaagtggac tgcaccgatt
     1501 ccc
//
LOCUS       DAOQMF010000561         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_67099_length_1503_cov_3.129834, whole genome shotgun sequence.
ACCESSION   DAOQMF010000561 DAOQMF010000000
VERSION     DAOQMF010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67099_length_1503_cov_3.129834"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="PLV77_08975"
     CDS             <1..1265
                     /locus_tag="PLV77_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNC16021.1"
                     /translation="KPWVRQEWADEYGLEDVNTPDFDRHIDSIWERSKVNHDCSELNQ
                     TQKTWIEAAEKLGWSWHKTDRNWDPEKHDPLVAGYMGWGDQSGAKQSTLKTYLQDAAD
                     HGAEIIVGCQAQKVLVENGRAAGVEATIEGVGRITVRSPRVVVAGGALESPALLLRSG
                     IGGPAAGKYLRLHPCTLIFATYPEDQQAWWGPPHAAVVDEFDQGLEGDGYGFLIEGAQ
                     YTTGLGGSAIPFTTAAEHREIMANYANGSITINLTRERGGGQVLIDGDGQSMPIYSLD
                     DPRDIANMHFGLEQQVRLHHAAGANQVFALATGMPGWRVGDDFEAFVDRVHRIPLRAG
                     GAKLFAAHQMGSCRMGKDPAESVADPRGELHDTPGVWIGDGSAFPTPSGTNPMITIMA
                     LASRTAEKILEDSGTSGRSASETVEAGA"
     gene            1262..>1503
                     /locus_tag="PLV77_08980"
     CDS             1262..>1503
                     /locus_tag="PLV77_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC16022.1"
                     /translation="MNPTEAPETEANEAAGGGEAAAPGWAAKTGSHFIGGEWRDPIST
                     DTIEVINSVTEEVMGTVPAGSAEDVDAAVAAARAALP"
BASE COUNT          268 a          526 c          509 g          200 t
ORIGIN      
        1 cgaagccctg ggtccggcag gaatgggccg acgagtacgg cctggaggac gtgaacaccc
       61 ccgacttcga ccgccacatc gattcgatct gggagcgttc gaaggtaaat cacgactgct
      121 cggagctgaa ccagacccag aagacctgga tcgaagcggc cgaaaagctc ggctggagct
      181 ggcacaagac cgaccgcaac tgggacccgg aaaagcacga cccgctggtt gccggctaca
      241 tgggctgggg tgatcagtcc ggggccaagc agtcgaccct caagacctac ctgcaggatg
      301 ccgccgacca cggcgccgaa atcatcgtcg gctgccaggc ccaaaaggtg ctggtcgaaa
      361 acggccgcgc ggccggggtc gaagcgacga tcgagggcgt cggccggatc acggtccgct
      421 cgccccgggt cgtcgttgcg ggcggcgcgc tcgaatcacc ggcgctgctc cttcgctccg
      481 ggatcggcgg cccggccgcc ggcaagtacc tgcggcttca cccctgcacc ctgatcttcg
      541 ccacctaccc ggaggaccag caggcctggt ggggtccgcc ccacgcggca gtcgtcgacg
      601 agttcgacca gggcctcgaa ggcgacggtt acggcttcct gatcgaaggc gcgcagtaca
      661 cgaccggcct cggcggctcg gcgatcccgt tcaccaccgc cgccgagcac cgcgagatca
      721 tggccaacta cgcgaacggt tcgatcacga tcaacctgac ccgcgagcgc ggcggcggcc
      781 aggtgctgat cgacggcgac ggccagtcga tgccgatcta ctccctggac gacccgcggg
      841 acatcgccaa catgcatttc ggtctcgagc agcaggtccg gctccatcat gccgccgggg
      901 ccaatcaggt cttcgccctc gccaccggca tgccgggctg gcgggtcggg gatgacttcg
      961 aagcattcgt cgaccgggtc caccggatcc cgctacgggc cggcggggcg aagctgtttg
     1021 ccgcccacca gatgggctcc tgccggatgg ggaaggatcc ggccgaaagc gtcgccgacc
     1081 cgcgtggcga gctgcatgac accccgggag tctggatcgg ggacggctcg gccttcccga
     1141 ctccctccgg caccaatccg atgatcacga tcatggctct cgcctcccgc accgccgaga
     1201 agatcctcga ggattccggg acgagcggcc gatcagccag cgagaccgtg gaggcgggag
     1261 catgaatccg accgaggccc ccgagaccga agccaacgag gccgccggcg gaggcgaggc
     1321 ggccgccccg ggctgggccg cgaagaccgg cagccacttc atcggcggcg agtggcgtga
     1381 cccgatctcg accgacacga tcgaggtgat caactcggtg accgaagagg tgatgggcac
     1441 cgttccggcc ggttcggccg aagacgtcga cgccgcggtc gcggccgccc gtgccgccct
     1501 gcc
//
LOCUS       DAOQMF010000562         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_67448_length_1499_cov_2.804017, whole genome shotgun sequence.
ACCESSION   DAOQMF010000562 DAOQMF010000000
VERSION     DAOQMF010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67448_length_1499_cov_2.804017"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLV77_08985"
     CDS             <1..643
                     /locus_tag="PLV77_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HNC16023.1"
                     /translation="LSNAHRQPPHESTPDTDLFLERLLGHPVEDTDAALREIEAIDPL
                     VAKLLADPMLGVTIAPTMASASSRENVIPSRAELVIDCRTPPGMTEEQVRARIDLVLG
                     DGDWRIEFNDPVVGNRSDYDGPLAEAIEEWVGLTEPGAEVLPQVMPGFSDSHWFRKAF
                     GATVFGFAPQRMSIAETKPLVHGKDERITVEDVELMENLYTWIPPRLLGTAES"
     gene            complement(840..1229)
                     /locus_tag="PLV77_08990"
     CDS             complement(840..1229)
                     /locus_tag="PLV77_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16024.1"
                     /translation="MEQKVEGSTETRGSERLGWKGLFTNHGPKFEVSRLVHQIVFGLF
                     LILGFVPVFLMSSHIHYPWNASFLEELAPMIVFMVYGAGLFSGAGRFTRWLFGPMPKR
                     EPYPTSPLPPQYSGQDQVSKETKPKDE"
     gene            1294..>1499
                     /locus_tag="PLV77_08995"
     CDS             1294..>1499
                     /locus_tag="PLV77_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC16025.1"
                     /translation="MFTRIDHIGVAVEDLDAAIKLYERNFEMELVHRETVESQGVEAV
                     LLDVGDGHVELLSPLGPDTPVGKF"
BASE COUNT          297 a          482 c          454 g          266 t
ORIGIN      
        1 gctttcgaac gcccatcgcc agccgccgca tgagtcgacc ccggacaccg acctcttcct
       61 cgaaaggctg ctcggccatc cggtcgagga caccgacgcc gccctgcgcg agatcgaagc
      121 gatcgacccg ctggtcgcca agcttttggc cgacccgatg ctcggagtga cgatcgcccc
      181 gaccatggcg agcgcttcca gccgcgagaa cgtgatcccc tcccgggccg agctggtgat
      241 cgactgccgc accccgcccg gcatgaccga agagcaggtc cgggcccgga tcgacctcgt
      301 actcggtgac ggcgactggc ggatcgagtt caacgacccc gtggtcggca atcgctccga
      361 ttacgacgga ccgctggccg aggcgatcga ggagtgggtc gggttgaccg aaccaggggc
      421 cgaggtcctg ccccaggtca tgcccggctt ctcggattcc cactggttcc gcaaggcctt
      481 cggcgccacc gtcttcggct tcgcccccca gcgcatgagc atcgccgaaa ccaagcccct
      541 cgtccacggc aaagacgaac gaatcaccgt cgaggacgta gagctgatgg aaaacctcta
      601 cacctggatc ccaccccgcc tgctcgggac cgcggagtcc tgacccggcc caggccgatc
      661 acgccccaca agattttcgc tgcgggaaac cgtggattag gacggtttgg gtcgacgggg
      721 cctgcgatcg gccgggtcga ccctcagctg ccaaggtcga gattcgcgac cgacaagctc
      781 gccagtgagg cagtgaacga gaaacaacgg gcggaattca tctgttgacg gttggtaatt
      841 cattcgtctt tcggtttcgt ttccttggaa acctgatcct gtcccgaata ctgaggtggc
      901 aacggtgatg tggggtacgg ctcgcgcttg ggcatcggcc caaaaagcca ccgggtgaac
      961 cggccagcgc cggagaacaa gccagcgccg tagaccatga agacaatcat cggcgcgagc
     1021 tcttcgagaa aagatgcgtt ccacgggtag tggatgtggc tcgacatcaa aaagaccggg
     1081 acgaacccga ggatcagaaa caatccaaat acgatctggt ggacgagccg ggacacctca
     1141 aactttgggc catgattcgt gaagaggccc ttccagccga gcctttcgct ccccctggtc
     1201 tcagtcgatc cttcgacttt ctgctccatg gccggaggat atccggcctc ggccggatgg
     1261 tgggataggc ctcatcgcta ggcttgtcgc ctcatgttca ctcgcataga tcacatcggt
     1321 gtggctgttg aggatctcga cgccgccatc aagctttatg agcggaattt cgagatggag
     1381 ctcgttcacc gcgaaaccgt cgaatcgcag ggagtcgagg cggtcctgct cgacgtcggc
     1441 gacgggcacg tcgaactgtt gagtccgctc ggccccgaca caccggtcgg caagttcat
//
LOCUS       DAOQMF010000563         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_67536_length_1498_cov_2.972280, whole genome shotgun sequence.
ACCESSION   DAOQMF010000563 DAOQMF010000000
VERSION     DAOQMF010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67536_length_1498_cov_2.972280"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1498)
                     /locus_tag="PLV77_09000"
     CDS             complement(<1..>1498)
                     /locus_tag="PLV77_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16026.1"
                     /translation="SNTEAFLPFGNRLWTSANNGKTGYEPWFVGNRKLKLIEDLWPGL
                     DASDPGPGAELNGFYYFPAYDPDHGNELWRFNGNSAELVKDIWPGSEDSTPREFVRVG
                     NRIFFVAHEPTSGRELWQIDAGGATLVADINSGTGTSNPSYLTAYLDKVYFSAYTAAH
                     GYELWSSDGTTTAEVGDLRPGSASSDPGSLTVAGGTLFMVANDGTNGYELFKSSGGPA
                     TLVADIQPGGNSSSPTSMLPFNNKLIFTANTSVSGSEPWITDGVSVTQIGDLAPGIDS
                     SYASYYSRMNGKVYFVAYLSDPRLYETDGVTARPTTAAFPEGASGFYNLAVVGNRLYL
                     SIGTPKTGLEPWVFDGTGARMIRDILPGPDSSYPTAYAKRGNGVIFAADDGTHGAEPW
                     LLHPDREVKLRPGSSRLGVSKQGFAGLKLTCPANEANGPCISKVTVSTRRPVVFRGKR
                     RKVTMARKSIRVLTGHTGTARLNFPKPIIRLLLHTPAARRATVTVKVHD"
BASE COUNT          251 a          436 c          533 g          278 t
ORIGIN      
        1 gtcgtgaacc ttgaccgtga ccgtggcgcg gcgggcggca ggggtgtgga gcagcaaccg
       61 gattatcggc ttcgggaagt tgagacgggc ggtgccggtg tggccggtca gaacccggat
      121 cgacttgcgg gccatggtca ccttgcgccg cttgccgcgg aagacgaccg gccgccgggt
      181 cgagacggtc accttcgaga tgcacggtcc gttcgcctcg tttgccgggc aggtcagctt
      241 cagtccggcg aagccctgct tcgagacgcc cagccttgaa cttcccggcc tgagcttcac
      301 ctcccggtcg gggtggagca gccagggctc ggccccgtgg gtgccgtcat cggccgcgaa
      361 aatcaccccg ttgccccgtt tggcgtacgc ggtcggatac gaggaatccg gaccgggcag
      421 gatgtccctg atcatgcggg cgccggttcc gtcgaaaacc cagggttcca acccggtctt
      481 cggagtgccg atcgacaggt agagccggtt gccgaccacg gccaggttgt aaaagccgga
      541 cgcgccttcc gggaaggcgg cggtcgtggg cctggccgtg acaccgtcgg tctcgtacag
      601 ccggggatcg gaaaggtagg cgacaaagta gaccttgccg ttcatccggc tgtagtagct
      661 ggcgtaggaa gaatcgatcc ccggggcaag gtcgccgatc tgggtgacgc tgacgccatc
      721 ggtgatccag ggttcggagc cggacacact cgtgttggcc gtgaatatga gtttgttgtt
      781 gaagggcagc atcgaggttg gtgaagatga gttgccgccg ggctggatgt cggcgaccag
      841 ggtggccggc ccgccggagc tcttgaagag ttcgtagccg ttggtgccgt cgtttgcgac
      901 catgaacagc gttccgccgg caactgtcag ggaacccggg tccgaggaag cggaaccggg
      961 gcggaggtca ccgacttcgg ccgtggtggt tccgtcgctc gaccacagtt cgtatccgtg
     1021 ggcggcggtg taggccgaga agtaaacctt gtccaggtac gcggtcaggt aactggggtt
     1081 cgaagtgccg gttcccgaat tgatgtcagc gaccagggtt gctccgccgg catcgatctg
     1141 ccagagctct cggccgctgg tcggctcgtg ggccacaaag aagatgcggt ttccgacccg
     1201 cacgaattcc cggggggtcg aatcttcgga gcccggccag atgtccttga ccagctccgc
     1261 ggaatttccg ttgaagcgcc agagctcgtt gccgtgatcg ggatcgtagg cggggaagta
     1321 atagaagccg ttcagctcgg ctcccgggcc ggggtcggag gcgtccaggc ctggccagag
     1381 gtcctcgatc agcttcagct tccgattgcc gacaaaccac ggctcatagc cggtcttgcc
     1441 attgttggca gaggtccaga gccggttccc gaacgggagg aacgcctcgg tgttcgag
//
LOCUS       DAOQMF010000564         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_67561_length_1498_cov_2.338184, whole genome shotgun sequence.
ACCESSION   DAOQMF010000564 DAOQMF010000000
VERSION     DAOQMF010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67561_length_1498_cov_2.338184"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PLV77_09005"
     CDS             complement(<1..538)
                     /locus_tag="PLV77_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16027.1"
                     /translation="MRAFHLLVAIQTLNAQIEPVVSKRNLSDQVQVVRVAPRFATAIR
                     VPEPVSSMVIGDPERFLAEHSEKEPTLVLVKPVTEQPGESNLLVVTTSGRQLSFLLRM
                     DPSGPKRVDFVLVYKPTGSFLVGESFPTSAEVARTDVLRPVSGLATPKASDAEKALDP
                     LQEILDRQKLAPLPTLYGG"
     gene            complement(535..990)
                     /locus_tag="PLV77_09010"
     CDS             complement(535..990)
                     /locus_tag="PLV77_09010"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNC16028.1"
                     /translation="MALVAGNVWAQSPALVALADRWAEHFGVPRELVRAVIEAESNWN
                     PRAVSSAGAVGLMQLMPNTAAAFRVGNRFDAAENIRGGVAYLAFLMEQCGGDLRLVVG
                     AYNAGHGRVLKAGLRYRNPETVNFTHRVAYLFRRNQWESMLTQRKEPAE"
     gene            complement(1008..>1498)
                     /locus_tag="PLV77_09015"
     CDS             complement(1008..>1498)
                     /locus_tag="PLV77_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16029.1"
                     /translation="SDWQITVPKLGTVKAQSVPFVVMTSNQERRLGDPLRRRSLYQRI
                     EHPDVKKEAEILDIRTVDAPLELKAQAVGLAQALRGYNLVKPPSIDEIVQFVRALQLL
                     GRSEIRPEDRDVLLPFLAKTEEDVKRLLLRDGFSSLVATAREYRDQALRAMSQRLEEA
                     VT"
BASE COUNT          293 a          477 c          441 g          287 t
ORIGIN      
        1 tgcctccgta aagggtgggg agtggggcga gtttctgccg gtccaggatt tcctgaagcg
       61 ggtcaagggc cttctcagca tcactagcct tcggagtcgc gagaccagag acgggtctga
      121 gaacgtcggt tctggcgacc tcagccgatg ttggaaacga ttcgcccacg agaaaactgc
      181 cggtcggctt gtagacgagc acgaagtcca ccctcttcgg gccgctggga tccatccgca
      241 agaggaaact cagttgcctg ccgctcgtag tcacaacaag caggttcgat tcgccaggtt
      301 gctcagtgac gggtttgaca aggacgaggg ttggctcctt ctcggaatgc tccgcaagga
      361 accgctcggg gtcaccgatg accattgaac tgactggttc gggaacgcga atcgccgtcg
      421 caaatcgcgg cgccacacgc accacctgca cttgatccga caggttccgc ttgctcacca
      481 ccggctcgat ctgggcgttg agcgtctgaa tcgcgacgag taagtgaaac gctctcattc
      541 agcaggctcc tttcgctggg tcaacatcga ttcccactga ttccgtcgga aaaggtaggc
      601 gacgcgatgc gtgaagttca ccgtttctgg gttcctgtat cgcagcccgg ccttcagcac
      661 tcgaccgtgg ccggcgttgt aggcgccaac gacgagacgc agatcgccgc cgcactgctc
      721 catcaggaac gcgaggtagg ccacaccgcc tcggatgttc tcggccgcat cgaaacggtt
      781 tccgacgcgg aaagctgccg cagtatttgg catcagttgc atcaagccga cagccccggc
      841 cgatgaaacc gcccgcggat tccagttcga ctccgcttcg atcaccgccc gcaccaattc
      901 tcttggtacg ccgaagtgtt cggcccagcg gtctgcgaga gccaccagcg ccggcgactg
      961 ggcccagaca ttgccggcga ctaacgccat cacgatgcac cgccaagtca cgtgacagcc
     1021 tcctctagcc tttgcgacat tgcccgcagg gcctgatcgc ggtactcacg tgccgtcgcg
     1081 acaaggctgc tgaatccgtc gcgaagcaag aggcgcttca cgtcttcctc ggtcttggcc
     1141 aggaacggga gtagaacatc ccggtcttcc gggcggatct cggagcgtcc cagaagctgc
     1201 agtgcccgga cgaactgcac gatttcgtcg atcgaaggcg gcttgaccag gttgtagcca
     1261 cgcaatgcct gcgcgagtcc gaccgcctgc gccttcaact caagcggggc atccaccgtt
     1321 cggatgtcga gaatctcggc ttccttcttc acatcgggat gctcgatccg ctggtagaga
     1381 cttcgccggc gcagcgggtc cccgagccga cgttcctggt tcgaagtcat aaccacgaac
     1441 ggcacggact gagccttcac ggtccccaac ttcggcacgg tgatctgcca gtcagaga
//
LOCUS       DAOQMF010000565         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_67982_length_1493_cov_3.323366, whole genome shotgun sequence.
ACCESSION   DAOQMF010000565 DAOQMF010000000
VERSION     DAOQMF010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67982_length_1493_cov_3.323366"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLV77_09020"
     CDS             <1..507
                     /locus_tag="PLV77_09020"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935013.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNC16030.1"
                     /translation="RAKAPRVIGDFTTLLGVLHALPLTYGKDMQEDKEPFFDAVETVE
                     ATLEMTAGIFEGIEFDRERLSAAASDEMLAATEIADLLVRRGVPFREAHGIVGDLVRQ
                     CVAEGRNLSDLSQDELASRSADLDEEYYEVLQQGSWLESKVSEGGTSSASLAAQIDLA
                     KARLREIG"
     gene            complement(526..>1493)
                     /locus_tag="PLV77_09025"
     CDS             complement(526..>1493)
                     /locus_tag="PLV77_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC16031.1"
                     /translation="HREPYSVSNYGDSYAGVSDLVSATINSDNSVYAQLGIEAIDGGP
                     KAIAKTIHEMGYSDKIETNPAMVLGTSEVTPLGWTYAFSTLANDGRRVSGTLAPDPGD
                     DPVSFTKITDEDGKTIKGGDNEVLSKQVIDPDVASEAKTILHEVVTQGTGKNAYIGDD
                     SQWGKTGTTEDNTNAWFCGAITEITACVWVGYPEGYVQMTTEYGGAPVDGGTFPAIIW
                     SRVVSAWKEIEANRAAERAADAADGDSSDSSDVTTDSSDYSYTAPSTDYTTPTYTAPE
                     TTTPVAPAPTPTTTAPAPTTTVPTTPTTPSTGGGVAPSSGGASPG"
BASE COUNT          219 a          459 c          526 g          289 t
ORIGIN      
        1 cgagccaagg caccgcgggt gatcggcgac ttcacaacgc tgctcggcgt gctccacgcc
       61 ctgcctctca cctacggcaa ggacatgcag gaggacaagg agccgttctt cgacgcggtc
      121 gagacggtcg aggcgacgct ggagatgacc gccggaatct tcgaagggat cgagttcgat
      181 cgcgagcgac tttccgctgc cgcctcggac gagatgctcg cggcgaccga gatcgccgac
      241 ctgctggtgc ggcgcggggt tcccttccgt gaggcgcacg gcatcgtcgg tgacctggtc
      301 cggcaatgtg tcgccgaagg ccggaacctc tccgacctca gccaggacga gctggcatcc
      361 cggtccgccg acctcgacga ggagtactac gaagttctgc agcagggcag ctggctcgaa
      421 tcgaaggtga gcgaaggtgg cacctcgagt gcaagcctcg ccgcccagat cgacctggcc
      481 aaggcccggc tccgcgaaat cggctgaggc cgtgcgctga cccggctaac cgggtgaagc
      541 gccgccggat gagggtgcga cgccaccgcc cgtggaaggc gtggtcggcg tggtgggcac
      601 cgtggtagtc ggtgcgggag ccgttgtcgt cggtgtcggg gccggagcga cgggggttgt
      661 cgtctccggg gccgtgtaag tcggcgtggt gtagtcggtg ctgggtgcgg tgtagctgta
      721 atcgctcgag tcggtggtca cgtcggatga gtcgctgctg tcgccgtcgg cggcatccgc
      781 tgcccgttcg gccgcacggt tggcttcgat ttccttccag gccgaaacca cccgggacca
      841 gatgatcgcc gggaaggtgc caccgtcaac cggggcgccg ccgtactcgg tggtcatctg
      901 tacatagcct tcggggtaac ccacccagac gcaggcggtg atctcggtga tcgcgccaca
      961 gaaccatgcg ttcgtgttgt cttcggtcgt gccggtcttg ccccactggc tgtcatcgcc
     1021 gatgtaggcg ttcttgccgg tgccctgggt gacaacctca tgcaggatgg tcttggcttc
     1081 gctggcgaca tccgggtcga tcacctgctt gctgagcacc tcgttgtcac ctcccttgat
     1141 cgtcttgccg tcctcgtcgg tgatcttggt gaagctgacc gggtcgtctc ccgggtccgg
     1201 cgcgagggtg ccgctgaccc ggcggccgtc attggccagg gtcgagaagg cataggtcca
     1261 gccgagcgga gtcacttcgg acgtgccgag caccatcgcc gggttggttt cgatcttgtc
     1321 ggagtagccc atctcgtgga tcgtcttggc gatcgccttg gggccgccgt cgatcgcttc
     1381 gatgccgagc tgggcgtaga ccgagttgtc ggagttgatg gtcgcgctga ccaggtcgct
     1441 tacgccggca taggagtcgc cgtagttgct gaccgaatag ggctcgcgat gcc
//
LOCUS       DAOQMF010000566         1493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_67993_length_1493_cov_2.924896, whole genome shotgun sequence.
ACCESSION   DAOQMF010000566 DAOQMF010000000
VERSION     DAOQMF010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67993_length_1493_cov_2.924896"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLV77_09030"
     CDS             <1..310
                     /locus_tag="PLV77_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16032.1"
                     /translation="QAVWKGDLGDLGDGPSTPKDLPLALGGNFVATFLLAQPDPNTKV
                     AGFAFAFQNLLAGSCEMLERVARLAGEDTIAAQAESRAAAVRNSANRISKLWEQLGGV
                     "
     gene            338..658
                     /locus_tag="PLV77_09035"
     CDS             338..658
                     /locus_tag="PLV77_09035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNC16033.1"
                     /translation="MSEVVVVAVLEVKEGRAEEAITAFTPVIEATHREPGCVSYALHQ
                     DNSDPNTLVLVERWKSQDDLNSHFTQPHMAEMGSLAAELLTGQPRIVFCSGIPKGDPA
                     KGVL"
     gene            709..>1493
                     /locus_tag="PLV77_09040"
     CDS             709..>1493
                     /locus_tag="PLV77_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC16034.1"
                     /translation="MEKGRGETLIIGGGIHGVSTALALASRGRKSLILDAAAPLEGAS
                     VHNEGKVHLGFVYALDEHGATTRSMVEGALAFAPLIERWCGEVDWAGLRSSPFAYLVM
                     NEGLAGAGQLEAHYESVLAEIGSAGASFGSNYLGVDPGRAEIVRHDGVFPGMAAGHSQ
                     CWFETPERSVRPVALAALLKRAAVDERAIEILPGHRVTRARRREAGFELEVETPEGPR
                     SIGAETVVNCAWEGRPALDRMILDQPSATNFRVKYSVIIHGEDT"
BASE COUNT          268 a          452 c          523 g          250 t
ORIGIN      
        1 gcaggccgtg tggaaagggg atctcgggga tctcggtgac ggaccgtcca cccccaagga
       61 cctgccgctc gcgctcggcg gcaacttcgt cgcgaccttc ctcctcgctc agcccgaccc
      121 gaacacgaag gtagccgggt tcgcgttcgc cttccagaac ctgctggctg gatcctgcga
      181 gatgctcgag cgggtcgcgc gactcgccgg cgaggacacg atcgccgccc aggccgagtc
      241 gcgtgccgct gccgtcagga actcggcgaa ccggatctcg aaactttggg agcagctcgg
      301 aggggtctga ccgtcgattc ccggataggg tcgggtcatg agcgaagtgg tcgtggtggc
      361 ggtactggaa gtcaaggagg gaagggcgga ggaggcgatc accgcgttta ccccggtgat
      421 cgaagcgacg caccgggaac ccggctgcgt ttcgtatgcc cttcatcagg acaactcgga
      481 cccgaacacc ctggtcctgg ttgaacgctg gaagtcccag gacgacctga attcacactt
      541 cacccagccg cacatggccg agatgggatc gcttgccgcc gaactgctca ccgggcaacc
      601 gcggatcgtc ttctgctccg gaatcccgaa gggagatccc gccaaggggg ttctctagcc
      661 tcgctcaggc cccttggcac ccggccggga aggaatacgc tggcggcagt ggagaagggg
      721 agaggggaaa cgttgatcat cgggggcggg attcacgggg tctcgaccgc gctggcgctg
      781 gcctccaggg gccggaagtc actgatcctg gatgcggcgg cgcctctcga aggcgcgagt
      841 gtgcacaacg aagggaaggt ccacctcggg tttgtctatg ccctcgacga gcacggggcc
      901 accacccgct cgatggtcga aggagcactc gccttcgctc cgttgatcga gaggtggtgc
      961 ggtgaagtcg actgggcggg cctgcgttcg agcccgttcg catatctggt catgaacgaa
     1021 ggcctggccg gagccggtca gctcgaagcc cattacgaat cggtgctggc cgagatcggg
     1081 agcgctggag cttccttcgg aagcaactac ctgggggtcg atcccggccg ggcggagatc
     1141 gttcgtcacg acggggtttt ccccgggatg gccgcgggtc actcgcagtg ctggtttgaa
     1201 actccggaaa ggtcggtgcg tcctgttgcg ctggccgctc tcctgaaacg ggcggcggtt
     1261 gatgaacggg cgatcgaaat cctcccagga cacagggtca cccgggcgcg gcggcgggaa
     1321 gccggatttg aactcgaggt ggaaacgcct gaagggcccc ggtcgatcgg agcggaaacg
     1381 gtggtgaact gcgcctggga aggccggccg gccctggacc ggatgattct cgaccagcct
     1441 tcggccacga acttccgggt caaatactcg gtgatcatcc acggggaaga cac
//
LOCUS       DAOQMF010000567         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_68791_length_1485_cov_2.694406, whole genome shotgun sequence.
ACCESSION   DAOQMF010000567 DAOQMF010000000
VERSION     DAOQMF010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68791_length_1485_cov_2.694406"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLV77_09045"
     CDS             complement(<1..296)
                     /locus_tag="PLV77_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HNC16035.1"
                     /translation="MAERIPRDQSLSRIYGSGALFAAAYGNVGSSIYYALGVTAAFAL
                     GLTPVAFMIAGLIFICTAATYAEATVMYPEAGGSSSFGRHAFNELVSFIAAWAQ"
     gene            complement(308..559)
                     /locus_tag="PLV77_09050"
     CDS             complement(308..559)
                     /locus_tag="PLV77_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16036.1"
                     /translation="MPELAMAAVAETALRGLGAVRKVASVERSNDQATFVLEVETEGD
                     LITEMRRAMPVPITVDKAPDGSLKVVLEWAWGRSAASAG"
     gene            587..766
                     /locus_tag="PLV77_09055"
     CDS             587..766
                     /locus_tag="PLV77_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16037.1"
                     /translation="MCRGSRSDGMLCCRRARGKRFNRGHEFLNPIGQRLNFKADSTYR
                     STEVIDGGGVRMSGF"
     gene            complement(867..>1485)
                     /locus_tag="PLV77_09060"
     CDS             complement(867..>1485)
                     /locus_tag="PLV77_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16038.1"
                     /translation="KYTEGTGPDFREGMNKFGPTEKHSTGTFQGIISDRGPIDGPGGV
                     ALASPTTFDLDWVWDFNKEGSSYCHVKATFRTQTDQITDPMARSVQIVWRGEANATAK
                     NTSSVVDFPGLGNVQAICQAGPNGYRRLIVDSAIGGSVYTREGSDDYKVNQGVGPLIM
                     RLPNNGMLFIQMDNGDRILVSSRWKVNDPVASNNWCVVSAQVYSP"
BASE COUNT          276 a          463 c          476 g          270 t
ORIGIN      
        1 atctgtgccc aggcggcgat gaagctgacg agttcgttga aggcgtgacg gccgaacgag
       61 gatgagccgc cggcctccgg gtacatcacc gtcgcctccg cgtaggtggc ggcggtgcag
      121 atgaagatca gcccggcgat catgaaggcg accggggtaa ggcccagcgc gaaggcggcg
      181 gttacgccga gcgcgtagta gatcgacgat ccgacgttgc cgtaggcggc ggcaaacagg
      241 gcgcctgaac cgtagatccg actcaggctc tggtcacgcg gaatccgttc agccaagact
      301 catcaactca gcctgcgctt gcggcagacc ttccccaggc ccattccaga accactttca
      361 acgatccgtc gggggcttta tcgacggtga tcggcaccgg catggcccgg cgcatctcgg
      421 tgatcaaatc gccttccgtt tcgacctcga gcacgaacgt cgcctgatca ttcgatcgtt
      481 ccacggacgc aactttgcga acagcaccga gtccgcgaag cgccgtctcg gcgaccgcgg
      541 ccattgccag ttccggcaag ggggaaacgc tgactactac ctcgccttgt gccggggcag
      601 ccgaagcgac gggatgctct gctgccggag ggcgaggggc aagagattca accgcggcca
      661 cgagttcctg aacccgatcg gccaacgcct gaacttcaag gctgactcga cctaccgaag
      721 cacggaggtc atcgacggcg gcggcgtccg catgtcggga ttctgaactc acggtcccat
      781 cctatctcca ccagagcccg cccatcaatc agttgccgct gtgggcgggc gggcttccgt
      841 tggtgcccgg cgaagaccgg gcgccgttaa ggtgaataga cctgggcgga aaccacgcac
      901 cagttgttgg aggcaaccgg gtcgttgacc ttccacctcg aagagaccag gatccggtct
      961 ccgttgtcca tctgaatgaa gagcatgccg ttgttgggca ggcgcatgat caacggaccg
     1021 acgccctggt tgactttgta gtcgtcggaa ccttcccggg tgtagacgct gccgccgatc
     1081 gccgaatcca cgatcaggcg acggtaaccg ttcggaccgg cctggcagat cgcctgcacg
     1141 ttcccgagtc cggggaagtc gacgacgctc gaagtgttct ttgcggtcgc gttggcctca
     1201 ccccgccaga cgatctggac cgaacgggcc atcggatcgg tgatctgatc ggtctgggtg
     1261 cggaaggtcg ccttcacgtg gcagtaggag ctgccttcct tgttgaaatc ccagacccag
     1321 tcaagatcga aggtggtcgg tgatgccagg gccacgccgc ccggtccgtc gatcggaccg
     1381 cggtcggaga tgatcccctg gaaggtgccg gtcgaatgct tctcggtcgg accgaacttg
     1441 ttcatgccct cgcggaagtc gggcccggtt ccttcggtgt acttg
//
LOCUS       DAOQMF010000568         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_69082_length_1482_cov_2.483532, whole genome shotgun sequence.
ACCESSION   DAOQMF010000568 DAOQMF010000000
VERSION     DAOQMF010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69082_length_1482_cov_2.483532"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..821
                     /locus_tag="PLV77_09065"
     CDS             171..821
                     /locus_tag="PLV77_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC16039.1"
                     /translation="MERSVWRYLFLAFLCASGTAALALLFYGSTRISEFDARVTSHFL
                     AVPDSRLDQVADFGARLAEPAPLILIAFGLIVLAVVWNRPWHLLAAGGVIIAANLTTQ
                     VMKALMAHPRLQGALGVSYPIEIHYPSGHTTAALASASGLWLVTPPRWRAWAAAFGAI
                     YALAVAAGVVIAGWHFISDVIGAALVVGFWACLAMAALVQAGLERTDRSRESSRTR"
     gene            complement(796..>1482)
                     /locus_tag="PLV77_09070"
     CDS             complement(796..>1482)
                     /locus_tag="PLV77_09070"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935946.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNC16040.1"
                     /translation="FVGGVPTLPVYRGELQARALGADLHAFGPDGEDLLGEVGELVLT
                     QPMPSMPICFWGDEDGSRLRESYFDTYPGIWRHGDWIEITERGTAIIYGRSDSTINRG
                     GIRMGTSEIYRAVLADEDIVDALVVDIPREGTEGWMPLFVVLAEGAELTDELKKKVAG
                     EIRSRCSPRHVPNEMIEIKAVPRTLSGKVLEVPVKRILTGTPAGKAASRDSLANPEAL
                     DFFSEYAKTL"
BASE COUNT          208 a          509 c          487 g          278 t
ORIGIN      
        1 cggatcctca caccgcggtc gcgtggagcg ttctcgacgc ggtgatccag cccgaagacc
       61 tccgcacgga actcatcaag cgcttcgccc tggccgaggg ccgtgaccgc tccttcaccg
      121 agcgccgcat tcccatcacc cccgcctgaa ggtttccgta gggtttccgg atggagcgat
      181 ccgtctggag atatctgttc ctcgcgtttc tctgcgccag tggaaccgcc gctctggcgc
      241 tgctgtttta tggctcaacc aggatcagcg agttcgatgc ccgggtcact tcccacttcc
      301 tcgccgtgcc ggactcccgg ctcgaccagg ttgccgactt cggggcgagg ctggccgagc
      361 cggctcccct gatcctgatc gcatttggct tgatcgttct cgccgtcgtc tggaaccggc
      421 cctggcacct gctcgcggcc ggcggggtga tcatcgccgc caacctgacg acccaggtga
      481 tgaaggcgct gatggcccat ccccggctcc agggggcgct cggggtcagc tacccgatcg
      541 agattcacta cccgagcggt cacaccaccg ccgcgctcgc ctcggcttcc ggcctctggc
      601 tggtgacgcc gccacgctgg cgggcctggg ctgcggcttt cggggcgatc tacgcgctcg
      661 ctgtggccgc cggcgtggtg attgccggct ggcacttcat cagcgacgtg atcggggcgg
      721 cactggtggt cggcttctgg gcctgcctcg ccatggccgc cctggtccag gccggccttg
      781 aacgaaccga tcgctctaga gagtcttcgc gtactcgctg aaaaagtcga gcgcttcggg
      841 gttggccagt gagtcgcggc tggccgcctt cccggccggg gtgccggtga ggatccgctt
      901 gaccggaact tcgagaactt tgcccgaaag ggtgcggggg accgctttga tctcgatcat
      961 ctcgttggga acgtgtcgcg gggagcagcg gctgcggatt tcgccggcga ctttcttctt
     1021 caactcgtcg gtcagttcgg ccccctcggc caggacgacg aagaggggca tccagccttc
     1081 ggttccttcg cgggggatgt cgaccacgag ggcatcgacg atgtcctcat cggccagtac
     1141 cgcccggtag atctccgagg tacccatgcg gatgccaccc cggttgatgg tcgagtcgga
     1201 gcggccgtag atgatcgcgg ttccccgctc ggtgatctcg atccagtcgc cgtgccgcca
     1261 gattcccggg taggtgtcga agtagctctc acgcagccgc gagccgtcct cgtcacccca
     1321 gaagcagatc ggcatcgagg gcatcggctg ggtcagcacg agctcgccga cctcgccgag
     1381 cagatcctcg ccgtcgggcc cgaaggcgtg caggtcggcg cccagcgccc gcgcctgcag
     1441 ctcgccgcga tagacgggca gcgtcgggac gccgccgacg aa
//
LOCUS       DAOQMF010000569         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_69734_length_1475_cov_2.955634, whole genome shotgun sequence.
ACCESSION   DAOQMF010000569 DAOQMF010000000
VERSION     DAOQMF010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69734_length_1475_cov_2.955634"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLV77_09075"
     CDS             complement(<1..818)
                     /locus_tag="PLV77_09075"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC16041.1"
                     /translation="MILLTGATGSIGSRLLPRLIEDGHQVRCLVREPRKLGAHRVDVQ
                     ITMGDLRELRDPYMLRQAMRGVDTVIHLAASIRDQPPYRVEELNGLATVRLLEAAERA
                     GVKRFVFFSALNASAVQRTRFFRAKWTAEQAVANSRLDSTIFRPSIVFDHSDPWITLL
                     RRFSFLPAIPISGNGKSRYQPVWADDVARAVENSLKGDGDGDHLYELAGPQTMTYAEM
                     SELVGLLAGRPRPVVRIPLQLVHLGLSAIHRLVGENAFATWQEAELMRVSMVTPR"
     gene            930..1193
                     /locus_tag="PLV77_09080"
     CDS             930..1193
                     /locus_tag="PLV77_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNC16042.1"
                     /translation="MSYVIAEPCIGQKDNSCVEVCPVDCIHPTPDEPGYDAVEMLFID
                     PDECIDCDACVEACPVDACFAEDQLPAEWAHFAEKNAQYFQNA"
BASE COUNT          265 a          463 c          498 g          249 t
ORIGIN      
        1 cggggagtga ccatcgagac gcgcatcagc tcggcctcct gccaggtcgc gaaggcgttt
       61 tcaccgacca gccggtggat tgccgaaaga cccaggtgga ccagctgcag cggaatccgg
      121 accaccggcc ggggccgacc ggccagcagc ccgaccagct cggacatctc ggcgtaggtc
      181 atcgtctgcg ggccggcgag ttcgtacagg tggtcgccat cgccgtcccc cttgagcgag
      241 ttctcgaccg cccgggcgac atcgtcagcc cagaccggct ggtagcggga cttgccgttg
      301 ccggagatcg gaatcgccgg caggaaggaa aagcggcgca gcagggtgat ccaggggtcg
      361 gaatgatcga agacgatcga gggccggaag atcgtcgagt cgagccgtga atttgcgacc
      421 gcctgctcgg ccgtccactt ggcccggaag aagcgggtcc gctggaccgc cgaggcgttg
      481 agggcactga agaagacgaa acgcttcacc ccggcccgct cggccgcctc gagcagccgc
      541 acggtggcca gcccgttcag ttcctcgacc cggtagggcg gctggtcccg gattgacgcg
      601 gcgaggtgaa tcaccgtgtc gaccccgcgc atcgcctggc gcagcatgta cgggtcacgc
      661 agttcgcgca ggtcccccat cgtgatctgg acgtcaaccc ggtgcgcgcc gagcttgcgc
      721 ggttcgcgaa cgaggcagcg gacctgatgg ccgtcttcga tcaggcgcgg cagaagccgc
      781 gagccgatcg agcctgtcgc tccggtcaaa aggatcacgg gcaggagtct aaagcgacgg
      841 gattgcgggc cgtttccgtg cgatcaggca gctaatcggc gggtcaacga gagggaggtc
      901 gggcggcctc aatctctaat atgggaacca tgagttacgt catcgcagag ccatgcatcg
      961 gtcaaaagga caactcctgc gtcgaggtgt gtccggtgga ttgcatccac ccgacccctg
     1021 acgagccggg ctacgacgcg gtggaaatgc tcttcatcga tcccgacgag tgcattgact
     1081 gcgacgcctg cgtcgaagcg tgcccggtcg atgcctgctt cgccgaggac cagctccccg
     1141 ccgaatgggc ccacttcgcc gagaagaacg cgcagtactt ccagaacgcc tgaggcttaa
     1201 ctgcagtcgc gccagcgggg ctggcgctcc catagctttc gcaccggccg gcaagaccga
     1261 cccggagcag atgcccgccg gcccacttga tcccgaattt gatgggccgg ctaacccgcc
     1321 cggcttttac tgaacgaggg gaagttctct ggcgttcttg cccattttgg gggcgatttc
     1381 gggggctgtg gtctgaatcg tttcgatcat cggtagctct atgggtgagg tggcgatgat
     1441 cttgcgggcg atttcgacga tcgcctgacc ggccg
//
LOCUS       DAOQMF010000570         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_69744_length_1475_cov_2.626056, whole genome shotgun sequence.
ACCESSION   DAOQMF010000570 DAOQMF010000000
VERSION     DAOQMF010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69744_length_1475_cov_2.626056"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLV77_09085"
     CDS             <1..708
                     /locus_tag="PLV77_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNC16043.1"
                     /translation="ADDVMTPRVKVKTLAPDQPVIAVIEAARAGGFSRFPVISEGSEI
                     VEGIVHVKHAVAVPHEDRDDVPIRDVMADPVLVPSTIELDPLLAELRKVGLQVAIVVD
                     EFGSFDGIVTLEDLIEEIVGEVRDEHDRDEDPVREIAQGIWAVSGLMRPDEVEDQIGV
                     RLPEDEDYETVAGLIGFELGRVPGRGDSVRVEATNEEKEPLLADLYVLRMDGLRVDRV
                     RLVVSKPQPEEDGEGEK"
     gene            705..>1475
                     /locus_tag="PLV77_09090"
     CDS             705..>1475
                     /locus_tag="PLV77_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNC16044.1"
                     /translation="MSTPLALMISLLLLAGNAFFVGAEFALISARRSVIELRADEGGW
                     AARITLHAMERVALMMAGAQLGITLCTLGLGALAEPAIAHALEHPFEGLGIPGSLVHP
                     AALVVALFLVGGFHVVLGEMVPKNIALAGPDRSALALGPPIAAVVKVLHPIIWVLNKT
                     ANGVLRLLGVTPSDEVTSAFTRDEVAGLVEESRREGMLDADETRLLTGALTFEERDAS
                     VVLLPIETMVTLPITVTPAEVEEAAGRTGFSRFPVTRDD"
BASE COUNT          231 a          469 c          522 g          253 t
ORIGIN      
        1 gcagacgacg tgatgactcc gcgggtcaag gtcaagaccc tggctccgga tcaaccggtg
       61 atcgcggtaa tcgaggcggc ccgggccggt ggcttttcgc gtttcccggt gatcagcgaa
      121 ggatccgaga tcgtcgaggg aatcgtccat gtcaagcacg cggtcgcggt ccctcatgaa
      181 gaccgcgacg atgtgccgat ccgcgacgtg atggccgatc cggtcctggt cccctcgacc
      241 atcgaacttg acccgctgct ggccgaactg cgcaaggtcg gcctgcaggt cgcgatcgtg
      301 gtcgacgagt tcggcagctt cgacggcatc gtcacgctcg aagacctgat cgaggagatc
      361 gtcggcgagg ttcgtgacga gcatgaccgg gacgaggatc cggtgcgtga aatcgcgcag
      421 ggaatctggg cggtttccgg gctgatgcgg ccggacgagg tcgaagacca gatcggggtg
      481 aggctccctg aagacgagga ttacgaaacc gtcgccgggc tgatcggctt cgaactcggc
      541 cgggtgcccg gccgcggcga ctcggtcagg gtcgaagcga ccaacgagga gaaggagccg
      601 cttctggccg acctctacgt gctgcggatg gacgggctca gggtcgaccg tgtgcggctc
      661 gtggtctcga agcctcagcc ggaagaggat ggggagggcg agaagtgagc accccgctgg
      721 ccctgatgat ttctctcttg ctgctggccg gcaatgcctt cttcgtcggg gccgagttcg
      781 cgctgatctc cgcccgccgg tcggtgatcg agctccgcgc cgacgaaggt ggctgggcgg
      841 cgcggatcac ccttcacgcg atggaacggg tagccctgat gatggccggc gcccagctcg
      901 gcatcaccct ctgcacgctc gggctgggcg ccctggccga gccggcgatc gcgcacgcgc
      961 tcgagcaccc cttcgagggc ctgggcatcc ccgggtcact ggtccatccg gcggccctgg
     1021 tggtcgccct gtttctggtc ggcggcttcc atgtggtgct cggcgagatg gtcccgaaga
     1081 acatcgccct ggccgggccg gaccgttccg cgttggcgct cggcccgccg atcgccgcgg
     1141 tggtcaaggt gcttcatccg atcatctggg tgctcaacaa gaccgcgaac ggggtcctgc
     1201 ggctgctggg cgtgactcct tccgatgagg tgaccagcgc cttcacccgg gatgaagtcg
     1261 ccgggctggt cgaggagtca cggcgtgaag gaatgctcga cgccgacgaa acccggctgc
     1321 tgaccggggc cctgaccttt gaagaacggg atgccagcgt ggttctgctc ccgatcgaaa
     1381 ccatggtgac cctgccgatc accgtcactc cggccgaggt cgaggaggcg gccggccgga
     1441 ccgggttctc ccgcttcccg gtcacccgcg acgac
//
LOCUS       DAOQMF010000571         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_69972_length_1472_cov_4.269584, whole genome shotgun sequence.
ACCESSION   DAOQMF010000571 DAOQMF010000000
VERSION     DAOQMF010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69972_length_1472_cov_4.269584"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..641)
                     /locus_tag="PLV77_09095"
     CDS             complement(180..641)
                     /locus_tag="PLV77_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16045.1"
                     /translation="MGTENISGKASRAYSSARENQHVRRLIEDEELRASLIAAAVAGR
                     KAFQRVQSNRSSAVESVTQDKKVKKELSSAAESLRDAADRLKAPPKKKRHPIRNLLLV
                     GVVTGGLVLVFSESARKSLLDAIFGAEEEFVYTSTTSTETAQTNGTPVGTE"
     gene            complement(714..>1472)
                     /locus_tag="PLV77_09100"
     CDS             complement(714..>1472)
                     /locus_tag="PLV77_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNC16046.1"
                     /translation="IIEEYTREAGVRNLEREIGTVCRKVARTVAEKGGKAKKVKVSGK
                     KVRELLGRRRVFSETRRRTTVPGVSTGLAWTPTGGDVLFIEATAMPGNGKLVITGQLG
                     EVMRESAQAALSYVRANARDLAPGLDEKWFAEHDLHIHVPAGAVPKDGPSAGVAITVA
                     LVSLITGRPVRNDVAMTGEVTLTGQVLPIGGVKEKSLAAQRAGIKIVILPGRNEGDVE
                     EIPEYERAEVEFFYADKVGDALAVALEGNLKGKQ"
BASE COUNT          235 a          489 c          452 g          296 t
ORIGIN      
        1 tcgagatcgg agttgggtgc gctatgtggc ggaaaatgga acctcgcggt ttccgcgatt
       61 gcccgcgaaa gcgggtcgag tttcgtcctc ttcggagaag agactccggg ccggcgagga
      121 tgcaaagaga aagggccccg gtttcccggg gccctttgaa actggttgcg gaaccgggtc
      181 tactcggttc cgaccggggt tccgttggtc tgggcggtct cggtggaggt cgtcgaggtg
      241 tagacgaact cttcctcggc gccgaagatc gcatcgagca ggctcttgcg ggccgactca
      301 ctgaagacca gaaccaggcc gccggtcacg acaccgacca ggagcaggtt gcggatcggg
      361 tggcgcttct tcttcggcgg cgccttgagg cggtcggcgg catcacgaag cgattccgcc
      421 gcgctcgaaa gttccttctt caccttcttg tcctgggtca ccgattcgac cgcggaactc
      481 cggtttgact ggacccgctg aaaggccttg cgaccggcca cggcggcagc gatcaggctc
      541 gcacgaagct cttcgtcctc gatcaggcga cggacatgct ggttctcgcg cgccgacgag
      601 tacgccctgg aggccttacc cgagatgttc tctgttccca tgtgttcctc gcttgatcga
      661 atggttgtct gtcgagaata gcggtacccc gctgcaaaga aaaacgaacc ggctcattgt
      721 ttgcccttga gattgccctc gagcgcgacc gccagggcgt ccccgacctt gtccgcatag
      781 aagaactcga cctcggcccg ctcgtactcg ggaatctcct cgacgtcgcc ttcgtttcgt
      841 ccgggcagga tcacgatctt gattcctgcc cgctgggcgg ccagcgattt ctccttgacg
      901 cccccgatcg gcagcacctg accggtcagg gtcacttcgc ccgtcatcgc gacgtcattg
      961 cgaaccggcc gcccggtgat cagggaaacc agcgctaccg tgatcgccac cccggccgaa
     1021 gggccatcct tgggcacggc gccggccggc acgtggatgt ggaggtcatg ttcggcaaac
     1081 catttctcgt cgaggcccgg cgccaggtcc cgtgcattgg cccgcacgta ggaaagtgcc
     1141 gcctgcgccg attccctcat cacctctccg agctgtccgg ttattaccag cttgccgttg
     1201 cccggcatcg ccgttgcttc gatgaagagc acgtcacccc cggtcggggt ccaggcgaga
     1261 ccggtcgaaa ccccgggaac cgtggttcgg cgtcgtgtct cggaaaacac ccggcgccgg
     1321 ccgagcagct cgcgcacctt ctttcccgag accttgacct tcttcgcctt gccgcccttc
     1381 tcggcgaccg tgcgggcaac cttccggcag acggtgccga tctcccgttc gagattccgc
     1441 accccggcct cccgggtgta ttcctcgatg at
//
LOCUS       DAOQMF010000572         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_70087_length_1471_cov_3.864407, whole genome shotgun sequence.
ACCESSION   DAOQMF010000572 DAOQMF010000000
VERSION     DAOQMF010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70087_length_1471_cov_3.864407"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLV77_09105"
     CDS             complement(<1..255)
                     /locus_tag="PLV77_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16047.1"
                     /translation="MAPTSKTVSSPPEAPERSLDQRMDALKRANNIRTARAQLKRDLK
                     AGRTTITEVLDEPPEYLLTAKVFDMLLAVPKYGRVKTNRIL"
     gene            complement(422..1339)
                     /locus_tag="PLV77_09110"
     CDS             complement(422..1339)
                     /locus_tag="PLV77_09110"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNC16048.1"
                     /translation="MDLSTGICGIPLSGPVLNGSGTFDALAARRVFGDALIEDFPFDC
                     YVSKTITMNPRPGNPPYRLWETPSGMINSIGLPGPGLDGFLENDLGALAEIGVPLIVS
                     VMADSAEDFATLVERVAARDEVAGIELNVSCPNVHSGLIVGEQPAETEALMKALRPLT
                     GKPLIVKLTPNVANPQEVAVAAEAGGADSVSMINTIKAAAIDPASSRTWLGAGAGGLS
                     GPAVRPIALAQVRAVSSAVGIPVVGMGGISDAESALEFILAGAEVIAVGTENFRNPRA
                     GAAVTEGLPLALRRRGFGSLAEARAATIG"
BASE COUNT          233 a          479 c          511 g          248 t
ORIGIN      
        1 gaggatccgg ttggtcttga ccctgccgta cttcggaacg gcgagcagca tgtcgaagac
       61 cttggcggtc aggaggtatt ccggcggctc gtcgagaacc tcggtgatcg tggtgcgacc
      121 ggccttgagg tcccgcttca gctgggccct ggcggtgcga atattgttgg ccctcttgag
      181 tgcgtccatc ctctggtcga gggacctttc cggggcttcc ggcgggctgg atacggtctt
      241 cgaggtcggg gccatggaag gcggcgagta tagccactgg cgagaagttt ggaggccgaa
      301 aacccccgaa aagtgccgat ttcgggcaaa tccatctcaa cctcgactcg aggttgaggg
      361 cgaggcggaa ccggtcaccg gccgggcatg tccggcgggc gcccgttcgg taagagccgg
      421 ctcagccgat cgtggccgcc cgggcctcgg ccaggctgcc gaacccgcgg cgccggagag
      481 caagcggaag accctcggtc acggccgccc cggcccgggg gttgcggaag ttctcggtac
      541 cgaccgcaat cacctcggcg ccggccagga tgaactcgag tgccgactcg gcgtcactga
      601 tgccgcccat gccgaccacc gggattccga ccgctgatga aaccgcccgc acctgggcca
      661 gggcgatcgg cctgaccgcc ggccccgaga gtccgccggc tccggcgcca agccaggttc
      721 ggctggaagc cgggtcgatc gcggccgcct tgatcgtgtt gatcatcgag accgagtcgg
      781 ctcccccggc ctcggccgca acagccacct cttgagggtt ggccacgttg ggcgtcaact
      841 tgacgatcag cggcttgccg gtcagcggcc gcagcgcctt catcagggcc tcggtctcgg
      901 cgggctgctc gccgacgatc agtccggagt ggacgttggg gcaggaaaca ttgagttcga
      961 tcccggccac ctcatccctg gccgcgaccc gctcgacgag ggtggcgaag tcttcggcgc
     1021 tgtcggccat gaccgaaacg atcaggggca ccccgatttc ggccagggcc cctaggtcgt
     1081 tttcgaggaa tccgtccagg cccgggccgg gcagtccgat cgagttgatc atccccgacg
     1141 gggtctccca gagccggtag ggcgggttgc cgggacgcgg gttcatggtg atcgtcttcg
     1201 agacgtaaca gtcgaagggg aagtcctcga tcagcgcgtc gccgaagacc cggcgggcgg
     1261 ccagcgcatc gaaggtcccc gaaccgttca gcacgggacc gctgagggga attccgcaga
     1321 tcccggtgga gagatccatg accgggagcg gttctgccgc ttttgccgat tcagtcaacg
     1381 gccggctctc ctgccatcgc ggtggcgatc tcgtcgccac ggacgaccgg gccgtcgatg
     1441 cagagtctca ggtagccgcc gtccgccttt g
//
LOCUS       DAOQMF010000573         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_70560_length_1466_cov_3.941885, whole genome shotgun sequence.
ACCESSION   DAOQMF010000573 DAOQMF010000000
VERSION     DAOQMF010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70560_length_1466_cov_3.941885"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="PLV77_09115"
     CDS             <1..1246
                     /locus_tag="PLV77_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16049.1"
                     /translation="VVGSKAPANPSDPADPAYSWGHYDTQIQNAVNAGLTVLVQINGA
                     PKWAERCKTQSTTATCNPNPVAFARFARAAATRYSGTYEGLPRIRYWEPLNEPNLESN
                     FLPQRRSSGKQSPVLYRSVLNRFAAAVKGVDPTNLVVGGGLSPLGGGNSYHPLAFARQ
                     LLCMQGRSRPTPIRGCSGTARFDIWASNPYTTGGPTHKSTNPDDIQLGDLSSMSRLLR
                     AARKAGKIRSSFKSIPYWLTEFSWDSNPPDPGGVKMPLLKRWTAHAMFQAWRSGVSAF
                     FWFSLRDIERTQGHPYAWTLESGLYQRSNSLSNDQPKGNLTAFRFPVVAFRRPGGIYF
                     WGRNPGGKSGKAVIRFRPPNGSGSRVLKRIKVGSGGVFQGFVRTRLGRNFKGSVFADS
                     GGQASVPFSLTTVKDFYQPPFG"
     gene            1263..>1466
                     /locus_tag="PLV77_09120"
     CDS             1263..>1466
                     /locus_tag="PLV77_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC16050.1"
                     /translation="MNLDALPGGKSILITGGAGFVGSSLAISLAESHPDRRIVALDNL
                     YRRGSELNLDRLGQAGVEFVKGDV"
BASE COUNT          246 a          512 c          450 g          258 t
ORIGIN      
        1 cgtggtcggc tcgaaagctc cggcaaatcc gtctgacccg gccgacccgg cctacagctg
       61 ggggcactac gacacccaga tccagaacgc ggtgaacgcc gggctgacgg tgctggtcca
      121 gatcaatggg gcgccgaaat gggccgaacg ctgcaagacc cagagcacca cggctacctg
      181 caatccgaat ccggtcgcct tcgctcgttt tgccagggcg gcagcgaccc gatacagcgg
      241 cacctatgaa gggctgcccc ggatccgcta ctgggagcca cttaacgagc ccaacctcga
      301 atcgaacttt ctgccgcaga ggcgcagctc gggcaagcag tccccggtcc tttaccggtc
      361 ggtgctgaac cggttcgcag ccgcggtcaa gggcgtggac cccaccaacc tggtggtcgg
      421 cggcggtctt tccccgctcg gcggcggcaa ctcctaccac ccgctcgcct tcgcccggca
      481 gcttctatgc atgcagggcc ggagcaggcc gaccccgatc cggggatgct ccggcacggc
      541 ccgcttcgac atctgggcct ccaacccgta cacgactggc gggcccaccc acaagtcgac
      601 caaccctgat gacatccagc tcggcgatct ctcgagcatg agtcggctgc tacgcgcggc
      661 ccgcaaggcc ggcaagatca ggtcgtcctt taagagcatt ccctactggc tgaccgagtt
      721 cagctgggat tcgaacccgc cggatccggg cggagtcaag atgccgctgc tcaagcgctg
      781 gactgcccac gcgatgttcc aggcctggcg ctccggcgtc tcggccttct tctggttctc
      841 gctgcgggac atcgagcgga cccaggggca cccctacgcc tggaccctcg agtcgggtct
      901 ctatcagcgc agcaactcgc tctcaaacga tcaaccgaag ggcaacctga cggcattccg
      961 cttcccggtg gtcgccttcc gtcgtcccgg cgggatctac ttctggggcc gcaaccccgg
     1021 tggcaagtca ggcaaggcgg tgattcgctt ccggccaccg aacggctccg gcagccgcgt
     1081 cctgaagcgg atcaaggtcg gcagcggcgg cgttttccag ggcttcgtgc ggacccgcct
     1141 cggtcggaac ttcaagggct cggtcttcgc cgactccggc ggtcaggcct cggttccctt
     1201 ctctctcacc accgtcaagg acttctacca gccgcccttc ggataaaact ccggattcca
     1261 ttgtgaatct ggacgcgcta cccgggggga aatccatctt gattacgggc ggagccggct
     1321 ttgtcggttc cagccttgcg atctcgctgg ctgagtcgca tcccgacagg cggatcgtcg
     1381 cgctggacaa cctttatcgc cgcggctccg agctgaacct cgaccggctg gggcaggccg
     1441 gggtcgagtt cgtcaagggt gacgtg
//
LOCUS       DAOQMF010000574         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_70740_length_1464_cov_6.189496, whole genome shotgun sequence.
ACCESSION   DAOQMF010000574 DAOQMF010000000
VERSION     DAOQMF010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70740_length_1464_cov_6.189496"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLV77_09125"
     CDS             <1..236
                     /locus_tag="PLV77_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HNC16051.1"
                     /translation="SGSHLELIPEVVRLLQEEGVEVPVVAGGIIPPEDAARLKEAGVA
                     GVFTPKDFDMNEIMGEFVDLVAANHEKSAAATA"
     gene            249..842
                     /locus_tag="PLV77_09130"
     CDS             249..842
                     /locus_tag="PLV77_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNC16052.1"
                     /translation="MNDQLYLLTHGIPGVIAGIMLALTLIGVSGSIWGIKRGLDGGEK
                     ILRVTAAILCLVGIGIAGYVVVTVEIMNQIPECVGGGGGCAAVEHSSYSRIGGIHVSI
                     FGLIGYTMLLVTSFMKGDLARIGAFFLALFGFGFSLYLTYLELWEILAICQWCVSSAI
                     TMTMLFVVNTSRLIVFFGTGESNGLAEGVEDPGPSSV"
     gene            855..1454
                     /locus_tag="PLV77_09135"
     CDS             855..1454
                     /locus_tag="PLV77_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNC16053.1"
                     /translation="MLEDQRFIISRDIPIEVGGLFILVALVGIGFAIWGYRTGGPRGE
                     QILRATAGVLSGLGLFVAGYIAYKTQIVNEPFQCAGGGGGCALVEKSKYANFLGVHMS
                     IWGLIGYATILGATIWKGDNARLAAFGLALFGFTVSLVLRYLELWEIHAACQWCWASA
                     VLMTVLLVVNSCRLIGYYGTGDPEQQTSFADEAEPDPAS"
BASE COUNT          236 a          434 c          479 g          315 t
ORIGIN      
        1 tttcaggatc gcacctcgag ctgattcccg aggtcgtgcg actgcttcag gaggagggcg
       61 tcgaggtgcc ggtggtcgcc ggcggcatca tcccgcccga ggacgcggcc cggctgaagg
      121 aagccggagt ggctggggtc ttcactccga aggacttcga catgaacgag atcatgggcg
      181 agttcgtcga cctggtcgcc gccaatcacg agaagtcggc ggccgcaacg gcctaggcag
      241 gaggttccgt gaatgatcag ctctatctgc tgactcacgg aatccccggt gtaatcgccg
      301 ggatcatgct ggcgttgacc ctgatcgggg tcagcggttc gatctggggg atcaaacgcg
      361 gcctcgacgg cggcgagaag atcctccggg tcaccgccgc gatcctctgc ctggtcggga
      421 tcggaatcgc cggttacgtg gtcgtgacgg tggaaatcat gaatcagatt ccggaatgcg
      481 ttggcggtgg cggcggttgc gccgcggtcg agcacagctc ctactcgcga atcgggggaa
      541 tccacgtttc gatcttcggc ctgatcggct acacgatgct gctggtcacc tctttcatga
      601 agggtgacct ggcccggatc ggcgctttct ttctggctct cttcggtttc gggttcagcc
      661 tctacctgac ctatctcgag ctttgggaga ttctcgcgat ctgccagtgg tgcgtttcct
      721 cggcgatcac catgaccatg ttgttcgtgg tcaatacgag caggctgatc gtgttctttg
      781 gcaccggcga gagcaacggg ctggctgaag gcgtggaaga ccctggcccg tcgtctgtgt
      841 aggctcgctt ccccatgctc gaagaccagc ggttcatcat cagccgggac attccgatcg
      901 aagtcggcgg cctcttcatt ctcgtcgctc tggtgggcat cggtttcgcg atatggggtt
      961 accggaccgg tggtccgcgc ggcgagcaga tcctccgtgc gacggccggt gtcctttccg
     1021 ggctcggact gttcgtggct ggctacatcg cctacaagac ccagatcgtg aacgagccct
     1081 tccagtgcgc cggcggcggt ggcggctgcg ccctggtcga gaagagcaag tacgcgaact
     1141 tcctcggggt acacatgtcg atctgggggc tgatcggcta cgcgacgatc ctcggagcga
     1201 cgatctggaa gggtgacaat gcccggctgg ccgccttcgg actcgcgctg ttcggcttca
     1261 ccgtcagcct ggtcctccgc tatctggaac tctgggagat ccacgccgcc tgccagtggt
     1321 gctgggccag cgcggtgctg atgaccgtgt tgctggtggt caattcctgc cgtctgatcg
     1381 gttactacgg aaccggagat ccggagcagc aaacctcatt tgctgacgag gcagagccag
     1441 atcccgcctc ttagtagcat cccc
//
LOCUS       DAOQMF010000575         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_70752_length_1464_cov_4.705465, whole genome shotgun sequence.
ACCESSION   DAOQMF010000575 DAOQMF010000000
VERSION     DAOQMF010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70752_length_1464_cov_4.705465"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLV77_09140"
     CDS             complement(<1..1204)
                     /locus_tag="PLV77_09140"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNC16054.1"
                     /translation="MPDGLSGPSGVSIEQGYLAIDDDSEVRLRRAGEALTLTVKSGHG
                     ESREETEVEVDQATFDDLWPESEGRRVEKLRRRIDLDQGLVAELDIYSGDLDGTSVVE
                     VEFESEAAARAFQPPAWFGRELTGDREWANQSLAVRGLPSKRDEFRLRAEEGPTTGVC
                     RVIAARAAQAAAAVRQAGEAEDSATPVHEARKSLKKVRSGLRLLRSVIPDDERTKVNE
                     TCRGAASRLSGARDAEVKIATLNSVTGPNPSPDGAGDWLGELEEEAEGHRGELNPGSL
                     AEVHDAIDGVRRTFLGRDPAAGPETVAGNAGRGYRRGRKAMKRANESGEAEDFHDWRK
                     RSKDLRYQLEILGPRLPEEFDQLRKEATDLADRLGDLHDLDVLAGDLESRDLEPEDKP
                     ALAELIGRA"
     gene            complement(1233..>1464)
                     /locus_tag="PLV77_09145"
     CDS             complement(1233..>1464)
                     /locus_tag="PLV77_09145"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936107.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC16055.1"
                     /translation="NEAFASVTLNTIKMLDIDPEKMNVNGGAVALGHPIGASGARVIG
                     ALVRELQRRGGGLGCAAICSGGGQGDAILVEV"
BASE COUNT          208 a          532 c          455 g          269 t
ORIGIN      
        1 gggcgcgacc gatcagttcg gccagcgcag gcttgtcttc cggttccagg tcccggctct
       61 ccagatcccc ggcgagcacg tcgaggtcat gcaggtcacc gagccggtcg gccaggtccg
      121 ttgcttcttt tcgcaactgg tcaaactctt ccggcaaccg gggcccgagt atctcgagtt
      181 gatacctcag gtccttcgac cgttttcgcc agtcgtggaa gtcttccgcc tcgccgctct
      241 cattcgcccg cttcatcgcc tttcgcccgc gccggtaacc acggccggcg ttcccggcga
      301 ccgtctccgg gccagccgcc ggatcccggc caaggaaggt gcgccggacg ccgtcgatcg
      361 cgtcatgaac ttcagccagg cttcccgggt tgagctcgcc gcggtggcct tccgcttctt
      421 cttcgagttc gcctagccag tccccggctc cgtccggcga cgggttcggg ccggtcaccg
      481 aattcagcgt ggcaatcttg acctcggcgt cgcgggcacc ggaaagccgg ctggctgctc
      541 cgcggcaggt ctcgttgacc ttcgtccgct cgtcatcggg gatcacgctt cgcagcagtc
      601 gcaggccgga acggactttc ttcaggctct ttcgtgcctc gtggacgggt gtcgcggaat
      661 cctcggcctc gcccgcctgg cgcaccgccg ccgcagcctg ggcggcccgg gccgcgatca
      721 cccggcagac cccggtcgtc gggccttcct cggctcgcaa tctgaactcg tcccgcttcg
      781 agggcaggcc gcgaactgcc agcgactggt ttgcccactc acggtcaccg gtcaactccc
      841 ggccaaacca ggcgggcggt tgaaaggccc gtgcggccgc ttccgattcg aactcgactt
      901 cgaccaccga ggtcccgtcg agatcacccg agtagatgtc cagctcggcg accaggccct
      961 ggtcgaggtc gatccggcgc cggagcttct cgacccgtct gccttccgat tccggccaga
     1021 ggtcgtcgaa ggtggcctgg tccacctcga cctcggtttc ctcccgcgac tcgccgtgac
     1081 cgctcttgac ggtcagggtc agcgcctcgc cggcccgcct caaccggact tcggaatcat
     1141 cgtcgatcgc caggtagccc tgctcgatcg aaactccgga cgggcccgaa agcccgtccg
     1201 gcacttcagg aacgaggaac ttccgctcta cctcagactt cgacgaggat ggcgtcaccc
     1261 tgaccaccgc cggagcagat cgcggcgcag ccaaggcctc cgccgcggcg ctgcagctcg
     1321 cgaacgaggg cgccgatcac ccgggcgccg gaagcgccga tcggatggcc gagggcgacg
     1381 gcgccgccgt tgacgttcat cttttcgggg tcgatgtcaa gcatcttgat cgtgttaagg
     1441 gtgaccgaag cgaaggcctc gttg
//
LOCUS       DAOQMF010000576         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_70766_length_1464_cov_3.740241, whole genome shotgun sequence.
ACCESSION   DAOQMF010000576 DAOQMF010000000
VERSION     DAOQMF010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70766_length_1464_cov_3.740241"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLV77_09150"
     CDS             <1..491
                     /locus_tag="PLV77_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNC16056.1"
                     /translation="PPGLRLDSGGIGKGLAADIVAERLEGFDSWAVECAGDLRFGGEG
                     GAERRIDVKSPFPGRGVIADFTASAGAVATSGTTRRSWKTGSGDSHHLIDPRTGRPAQ
                     TGLVQVTAVAPTAVEAEVRAKAALLSGPDRASEWLIDGGMFVTEEGEIRAWDFQRRPE
                     VG"
     gene            484..1086
                     /locus_tag="PLV77_09155"
     CDS             484..1086
                     /locus_tag="PLV77_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNC16057.1"
                     /translation="MGNGIHIFWVLSRGAGIAAILFAGLSVTVGLLSSRGMPFPNLRK
                     NFELRPLHEALSMATILLVAAHGLLLLGDPWLKPGITGISVPFVMSYRSFWTGIGIIA
                     GYGLILLGLSYYLRRRIGPSRWRTAHRFIAIFWLIGLAHTFGAGTDASQPWVWVPVAL
                     TSAPALVLLIMRLSRQGSKPAPARVAGTPVSDRAVMIDRH"
     gene            1104..>1464
                     /locus_tag="PLV77_09160"
     CDS             1104..>1464
                     /locus_tag="PLV77_09160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC16058.1"
                     /translation="MDQSGVAEAVAWAESEGWQPGLGDHLAFFAADPEGFFHSQLDGQ
                     TVATISVVRGSPEIAFVGLYIVAAEFRGRGFGRQLWDEALGKFDGLTLGLDAVPEQEA
                     TYASDGFASAYGNARFSA"
BASE COUNT          213 a          482 c          493 g          276 t
ORIGIN      
        1 gcccgccggg cctgcgtctc gattccggcg ggatcggcaa ggggctggcc gccgacatcg
       61 tcgccgagag gcttgaggga ttcgactcct gggcggtcga atgtgccggc gacctgcgat
      121 tcggaggcga gggtggtgcc gagcgccgga tcgacgtcaa gtcacctttc cccggccggg
      181 gcgtgatcgc cgacttcacg gcctccgcgg gagcggttgc caccagcggc acgacccgca
      241 gatcatggaa gaccggttct ggcgactccc atcatctgat cgacccccgc accggccggc
      301 cggcgcaaac cggcctggtg caggtcacgg cggtcgcgcc gaccgccgtg gaagccgagg
      361 tgcgtgccaa ggctgcgctg ctttccgggc ccgaccgggc ttccgaatgg ttgatcgacg
      421 gcgggatgtt cgtgaccgaa gaaggcgaga tccgggcctg ggatttccag agaaggccgg
      481 aagttgggta acgggatcca catcttctgg gtcctcagcc gcggcgccgg catcgccgcg
      541 atcctcttcg ccggactctc ggtgaccgtc ggcctgctgt ccagccgcgg catgccgttt
      601 ccgaacctga gaaagaactt cgagctgagg ccgctccacg aggcgctctc gatggcgacg
      661 atcctgctgg tcgctgccca cggcctgctc ctgctcggtg acccctggct gaaacccggg
      721 atcaccggta tctcggttcc cttcgtgatg tcgtaccggt cgttctggac cggaatcggg
      781 atcatcgccg gctacgggct gatcttgctg gggctcagct attaccttcg ccgccggatc
      841 ggaccctcgc gctggcgcac ggcccaccgc ttcatcgcga tcttctggct gatcggtctt
      901 gctcacactt tcggcgccgg tactgacgcg agccagccct gggtctgggt tcccgtagcg
      961 ctcacttcag cgcccgccct ggtcttgctg atcatgcggc tgtcgcgtca gggcagcaaa
     1021 cctgccccgg cccgggttgc cggcaccccg gtttccgacc gagcggtcat gattgaccgg
     1081 cattgaatcc tcacatcaaa gcgatggatc aatcaggggt tgccgaggca gtcgcctggg
     1141 cagaaagcga gggctggcag cccggtctcg gtgaccacct ggcgttcttc gcggctgacc
     1201 cggaaggctt ctttcactcg cagctagacg ggcagacggt cgccacgatc tcggtggtcc
     1261 ggggatcgcc ggagattgcc ttcgtcgggc tttacatcgt cgccgccgag ttccgcggcc
     1321 gcggtttcgg caggcagctt tgggatgagg ccctgggaaa gttcgatggt ttaacgctgg
     1381 gcctcgacgc ggtcccggaa caggaagcga cctacgcctc ggacggtttc gcttcggcgt
     1441 atggcaacgc caggttctcg gcgg
//
LOCUS       DAOQMF010000577         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_70788_length_1464_cov_2.882896, whole genome shotgun sequence.
ACCESSION   DAOQMF010000577 DAOQMF010000000
VERSION     DAOQMF010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70788_length_1464_cov_2.882896"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PLV77_09165"
     CDS             complement(<1..163)
                     /locus_tag="PLV77_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNC16059.1"
                     /translation="MEWRSAGEYSDILYEKAEGIAKITINRPEVRNAFRPQCLAELRD
                     AFEKARDDTE"
     gene            297..>1464
                     /locus_tag="PLV77_09170"
     CDS             297..>1464
                     /locus_tag="PLV77_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC16060.1"
                     /translation="MKLDNWLAQRSQSCPDRIALIADGESMTYAQLEEEAVHAARRLA
                     AWGVRRGATVAMNMRPGRDKIILVHALMKTGAVLLPISPKLSPEERAKAIESCDVSVD
                     LDDPGKLTQTEADMPLLGEHDMSDPHCRILTSGSTGRPHTVSLSYGNHLFSAMGSAFN
                     IGVDPSDRWLCALPTSHISGLSIVMRSVIYGTAMVLQDHFEPEAITDAIERDGATVIS
                     LVSTMLLRLLDEGVDLTAPRAILVGGGPVPASALSEALDRGARVIQTYGLTETCSQVT
                     TLEVAEARRKVGSVGRPLLTSHLRIQGGEILVQGPTVSAGALDADGWLHTGDVGWIDE
                     DGFLYVQGRTDEMIITGGENVIPAEVEDVLVQHPEILEAAVIGRPDPEWQQAVTA"
BASE COUNT          246 a          496 c          472 g          250 t
ORIGIN      
        1 cttcggtgtc gtcccgggct ttctcgaagg cgtcacgcag ctccgcgagg cactggggcc
       61 ggaacgcgtt ccggacctcg ggccggttga tggtgatctt cgcgatacct tcggccttct
      121 cgtagaggat gtcggagtat tctccggccg accgccattc gatcggctcg taaagcgtct
      181 cttccggggg tgcgttgcgg tgtctttcgg gcaagtccgg tactccgttc gtcaggccgg
      241 agccaggatg gctcgcggca agcaagattt gagagcacca cgataaacga ggtcaattga
      301 aactcgacaa ctggctggcc cagagatcgc agagctgccc cgaccggatc gccctgatcg
      361 cggacggcga gtcaatgacc tacgcccagc tcgaagagga agcggtccat gcggcccggc
      421 gccttgccgc ctggggagtg cgccgcgggg cgaccgttgc gatgaacatg cggccgggcc
      481 gggacaagat catcctcgtc catgccctga tgaagaccgg cgccgtgctg ctgccgatca
      541 gcccgaagct ttcaccggag gaacgggcaa aggcaatcga gtcctgtgac gtctccgtcg
      601 acctcgacga ccccgggaag ctcacccaga ccgaagccga catgccgctg ctcggtgagc
      661 acgacatgtc cgacccgcat tgccgcatcc tgaccagcgg gtcgaccggc aggccccaca
      721 ccgtttccct ttcctacggc aaccatctct tcagcgcgat gggctccgcc ttcaacatcg
      781 gcgtcgaccc ctccgaccgc tggctgtgtg cgctgccgac cagccacatc tccgggctct
      841 cgatcgtgat gcgctctgtg atctacggca ccgcgatggt gctccaggac cacttcgaac
      901 cggaggcgat caccgacgcg atcgagcgcg acggggcgac cgtgatctcg ctcgtgtcga
      961 cgatgctgct caggttgctc gacgaggggg tcgacctcac cgctcctcgc gcaatcctgg
     1021 tcggaggcgg accggtgccg gccagcgccc tgtcggaagc tctcgaccgc ggtgcccggg
     1081 tgattcagac ctacgggctg accgagacct gctcccaggt gaccaccctc gaagtggccg
     1141 aagcgcggcg caaggtcggt tcggtcggcc gccccctgct cacttcgcac ctgcggatcc
     1201 agggtggcga gatcctggtt cagggcccga ccgtttcggc cggcgccctc gacgccgacg
     1261 gctggcttca cactggtgac gtcggctgga tcgacgagga cggcttcctc tacgttcagg
     1321 gccggaccga cgagatgatc atcaccggcg gtgaaaacgt cattccggcc gaggtcgagg
     1381 acgtgctggt ccagcacccg gagatcctcg aagcagccgt catcggccgg cccgacccgg
     1441 aatggcaaca ggccgtaacg gcgg
//
LOCUS       DAOQMF010000578         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_70861_length_1463_cov_3.321023, whole genome shotgun sequence.
ACCESSION   DAOQMF010000578 DAOQMF010000000
VERSION     DAOQMF010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70861_length_1463_cov_3.321023"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLV77_09175"
     CDS             <1..288
                     /locus_tag="PLV77_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16061.1"
                     /translation="EQLAGTRAAPHSDQISAAVTAGQTEQAISHIPGSVGAAQIHQIR
                     EAAHFAFVGGMNTLFLVTSAIALIGSVGAFFLVRQSDFVAPPGQEGGTANE"
     gene            384..>1463
                     /locus_tag="PLV77_09180"
     CDS             384..>1463
                     /locus_tag="PLV77_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16062.1"
                     /translation="MKRATGCVIAVFVFCLLGASSASGYLVYYKEGNTNVDTGSGLVW
                     AVKPSATGQNNWWWYINNNLNVDTAVCYRYQIDGGGWKAPNGSNYSAPTGNNTGTLPA
                     ANASICEGPYTDGTKGPLQFAKSLTGLVNGQIATYCAEEWFKSGGDWDNSADPEQCSS
                     VKIDTVSPTITVDLRQGGNPVSVTNSPGSVTDNIGYSDSASQPWNRGSVWGDNRLCTT
                     TSMACNSGNTFNFSATCSNGTKSLSQTFTCPMGTPPGDGTFYQCIASFDGAVLDTYPS
                     LIADNVAGQPNTFTNDDPTGIGNSPANTIYPQTFNAGTGNYGIGCDSVLIDTAAPNTS
                     ITGGPAEGSSSADTTPTFTFTSTEAG"
BASE COUNT          307 a          518 c          404 g          234 t
ORIGIN      
        1 gaacagctgg ccggaaccag ggcggctccg cacagcgacc agatctccgc cgcggtcacg
       61 gccggccaga ccgagcaggc gatctcccac atccccggtt cggtcggggc agcacagatc
      121 caccagatcc gggaagccgc ccacttcgcc ttcgtcggcg ggatgaacac gctcttcctg
      181 gtgaccagcg cgatcgccct gatcggctcg gtcggcgcct tcttcctggt ccggcaaagc
      241 gacttcgtcg ccccgcccgg tcaagagggt ggaaccgcca acgaataacc gctcaatccg
      301 gtactccgga ttgctggcaa tgcgcctcga ccgacgctag tctggatgga ccatcgggat
      361 ccggacgcat tgggggagtc gtcatgaagc gcgcaacggg ttgtgtcatc gcagtcttcg
      421 tcttctgcct gctcggcgcg agttcagcct ccggctatct cgtctactac aaagagggca
      481 ataccaacgt cgataccggc tccgggctcg tctgggccgt taagccaagt gccaccggcc
      541 agaacaactg gtggtggtac atcaacaaca acctcaacgt ggacacggcg gtctgttacc
      601 gctaccagat cgacggtggt ggctggaaag ctcccaacgg aagcaactac tcagctccga
      661 ccggcaacaa caccggcacc ctgcccgccg ccaatgcctc gatctgtgag ggcccctaca
      721 ccgatggaac caagggcccc cttcaattcg ccaagagcct gaccggactc gtcaacggac
      781 agatcgcgac ctactgcgcc gaagagtggt tcaagagcgg cggggactgg gacaactcgg
      841 ccgacccgga gcaatgttcg agcgtcaaga tcgacacggt ttccccgacg atcaccgtcg
      901 acctcagaca aggcggaaat cccgtgtcgg ttacgaactc gccgggttcg gtgacggaca
      961 acatcggcta ctcggattcc gccagccagc cctggaaccg gggatcggtc tggggtgaca
     1021 accgcctttg cacgaccaca agcatggcct gcaattccgg caacaccttc aacttctcgg
     1081 ccacctgttc gaacggcacc aagtcactca gccagacctt cacctgcccg atgggtaccc
     1141 cgcccggcga cggaaccttc tatcaatgca ttgccagctt cgatggggct gtcctcgaca
     1201 cctacccgtc actcatcgcg gacaacgtcg ccggtcagcc caacacattc accaacgacg
     1261 acccgaccgg gatcggcaat agcccggcca acacgatcta tccccaaact ttcaacgccg
     1321 gcaccggcaa ctacgggatc ggctgcgaca gcgtgttgat cgacaccgcc gccccgaaca
     1381 cctcgataac cggcggcccg gccgagggct cgagcagcgc cgacaccacc ccgaccttca
     1441 ccttcacctc gaccgaagca ggt
//
LOCUS       DAOQMF010000579         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_70878_length_1463_cov_2.963778, whole genome shotgun sequence.
ACCESSION   DAOQMF010000579 DAOQMF010000000
VERSION     DAOQMF010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70878_length_1463_cov_2.963778"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLV77_09185"
     CDS             <1..550
                     /locus_tag="PLV77_09185"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNC16063.1"
                     /translation="VSDRIGKTTPYVETDQTGPVSSYGVSKLAGEVATLNANRRCFVV
                     RSSWLFGINGGNFVDTMIRLGKTQKQVLVVRDQVGCPTYTWHLAYGLVRLLDSDAYGI
                     HHMAGGGNCSWYDFAKEIFKLSEMEVTTLSATTEMFAAKAPRPAWSVLESGIEHAITL
                     PDWQDGLAAYLEQRRSLEGTGS"
     gene            547..>1463
                     /gene="rfbB"
                     /locus_tag="PLV77_09190"
     CDS             547..>1463
                     /gene="rfbB"
                     /locus_tag="PLV77_09190"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNC16064.1"
                     /translation="MKLLVTGGAGFIGSNFVHHMVESGKGDGLVVLDKLTYAGRPENL
                     EGLDVELVEGDICDRDLVRGLVDRVDAVVNFAAESHVDRSIESPGEFITTDVFGTFVL
                     LEAARDAGIRHLQISTDEVYGSIDEGSFTEASPIVPSSPYSASKAGGDLIVGAFQHTY
                     GADTLIARASNNYGPQQHPEKLIPLAILNAIHDDPIPVYGDGSQVRNWLFVRDFCAAI
                     EQVLDQGEAGQVYNVGGPDELPNIQVIRTILELTGRDESLITHVGDRLGHDVRYSLAS
                     KKTEGLGWEAAVGFREGIGRVGTKPVGCE"
BASE COUNT          252 a          487 c          452 g          272 t
ORIGIN      
        1 ggtctccgat cggatcggca agaccacgcc ctatgtcgag accgatcaga ccggtccggt
       61 ctcgtcctac ggcgtctcga aactggccgg cgaggtcgcg accctgaacg cgaaccggcg
      121 ctgtttcgtg gtccgctcct cctggctttt cggcatcaac ggtggcaact tcgtcgacac
      181 tatgatccgg ctcggcaaga cccagaagca ggtgctggtg gtccgcgatc aggtcggctg
      241 cccgacctac acctggcatc tcgcctatgg gctggtccgt ctgctcgact ccgatgccta
      301 cggcatccat cacatggccg ggggcgggaa ctgctcctgg tacgacttcg ccaaggaaat
      361 cttcaaactg tcggaaatgg aagtcaccac cctttccgcg accaccgaga tgttcgccgc
      421 caaggcgccc cgaccagctt ggtcggtgct tgaaagcggc atcgaacacg cgatcaccct
      481 gcccgactgg caggacggcc ttgccgccta cctcgagcag cgccgcagcc tcgaagggac
      541 tggctcgtga agctgctggt caccggcggg gccggcttca tcggctccaa tttcgttcat
      601 cacatggtcg agtccggcaa gggcgacggc ctcgtcgtgc tcgacaagct gacctatgcc
      661 ggccggccgg agaatctcga agggctcgac gtcgagctgg tcgaaggcga catctgcgac
      721 cgggacctgg tccgcggtct ggtcgaccgg gtggacgcgg tggtcaactt cgcggccgaa
      781 tcccatgtcg accgctcgat cgaatcgccc ggcgagttca tcaccaccga tgtcttcggc
      841 accttcgtgc tgcttgaagc ggctcgcgac gccggtatcc gtcatctcca gatctcgacc
      901 gacgaggtct atgggtcgat cgacgagggc tccttcaccg aggcttcccc gatcgtcccc
      961 tcctcgccat attcggcctc gaaggccggt ggcgacctga tcgtcggcgc tttccagcac
     1021 acctacggag ccgacaccct gattgcccgg gcctcgaaca attacggccc gcagcagcat
     1081 cccgagaagc tgatcccgct ggccattctc aacgcgatcc acgatgatcc gatccccgtt
     1141 tacggtgacg gcagccaagt ccgtaactgg ctgtttgtcc gcgacttctg cgcggcgatc
     1201 gagcaggtgc tcgaccaggg tgaggccggt caggtctata acgtcggggg tccggatgag
     1261 cttccgaaca tccaggtgat ccggaccatc ctcgagctga ccggcaggga cgagtcgctg
     1321 atcacccatg tcggtgaccg gctcggacat gatgtccgtt attcgctcgc ctcgaagaag
     1381 accgagggtc tcggctggga agccgcggtc ggcttccgcg aggggatcgg ccgggtcggc
     1441 accaagccgg tcggctgcga gga
//
LOCUS       DAOQMF010000580         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_71229_length_1459_cov_3.430199, whole genome shotgun sequence.
ACCESSION   DAOQMF010000580 DAOQMF010000000
VERSION     DAOQMF010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71229_length_1459_cov_3.430199"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PLV77_09195"
     CDS             complement(<1..136)
                     /locus_tag="PLV77_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC16065.1"
                     /translation="MSLFKRIVVGTDGSESAREAIRQAAELARMSGASLDVVSAFEPV
                     P"
     gene            complement(189..338)
                     /locus_tag="PLV77_09200"
     CDS             complement(189..338)
                     /locus_tag="PLV77_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16066.1"
                     /translation="MPANWRILMIVICVFLVASVTIGAIQLLSTPTEIFGAGFEGLPK
                     QEFPR"
     gene            389..1132
                     /locus_tag="PLV77_09205"
     CDS             389..1132
                     /locus_tag="PLV77_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC16067.1"
                     /translation="MNANRVAPKGLEEVADGVWLLTGDIKGAMNIYFIEGPDGEIVQF
                     DAATKSMVKANLRVIREFGGIDRVVLGHAHADHRGTAPYLGVPVHCHPDEVADATSSA
                     SIAPYMYLKELEFAPVRWIYPFLLRRWDGGPVEIAGTVEEGDEIAGFKVIHFPGHAPG
                     LIGLWRESDRVALVSDTVYFVDSARMRPLPEGEASVPHPAWAWDHQKAKESVRKLAGL
                     GPKVVAAGHEHPLRGEDLTDVLLRAADRH"
     gene            1174..>1459
                     /locus_tag="PLV77_09210"
     CDS             1174..>1459
                     /locus_tag="PLV77_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16068.1"
                     /translation="MLLIVAGSLVTGRAITRACGQEKWWGIEPAVGFAALMAVEGLLA
                     RVPGTRAALIAGLVVLAAASLWILRRPRREDLPGSPFFWVARATRSGSGGV"
BASE COUNT          257 a          458 c          504 g          240 t
ORIGIN      
        1 ccggaaccgg ctcgaaggcg ctcacgacat cgagcgaagc ccccgacatc cgggccagtt
       61 cggccgcctg acggatcgct tcgcgggccg actcggaacc gtcggtaccg acaactatgc
      121 gcttgaacag ggacattggt ctccgatcgt cgtgggcggt caccggcatc ctacccccgg
      181 gaccagtcct atctgggaaa ctcctgcttt ggcaaaccct caaaaccggc accgaagatt
      241 tcggtcggag tggaaagcaa ctggattgcg ccgatggtga ccgaggccac gaggaatacg
      301 cagatcacga tcatcagaat tctccagttg gccggcacgg gattcaggtt atggaaaaga
      361 cgctcaagat gacaccggaa gccagcaagt gaacgcgaat cgggtagcgc cgaagggact
      421 cgaggaagtg gcggacggag tctggctgct gaccggcgac atcaagggcg cgatgaacat
      481 ctacttcatc gaaggcccgg acggggagat cgtccagttc gacgcggcca cgaaatcgat
      541 ggtcaaggcc aacctgcggg tgatcaggga attcggcggt atcgacaggg tcgtgctcgg
      601 ccacgcgcac gccgaccatc gcggcaccgc cccctacctc ggcgtgccgg tccattgcca
      661 tcccgacgag gtcgcggatg ccacttccag cgcctcgatc gcgccctaca tgtacctgaa
      721 ggaactcgag tttgccccgg tccgctggat ctaccccttt ctgctgcggc gttgggatgg
      781 cggtccggtc gagatcgccg gaaccgtcga ggaaggcgac gagatcgcgg gcttcaaggt
      841 gatccacttc cccgggcatg ccccgggttt gatcggactg tggcgtgaat cggaccgggt
      901 cgccctggtc agcgacaccg tctacttcgt ggattcggcc cggatgagac cgttgccgga
      961 gggcgaggcg tcggtaccgc atccggcctg ggcctgggac catcagaagg ccaaggagtc
     1021 ggtgcgcaaa ctggccggac tggggccgaa ggtggtggcc gccgggcacg agcacccgct
     1081 ccgtggcgag gacctgaccg acgtgctgct cagggcagcc gacaggcact agctccgggg
     1141 cgactggcag ggatgttcta cagctggctc cccatgcttc tgatcgttgc cgggtccctg
     1201 gtgaccggcc gcgcgatcac ccgcgcctgc ggccaggaga agtggtgggg aatcgaaccg
     1261 gcggtcggct tcgcggccct gatggcggtc gagggcctgc tggcccgggt tccgggaacg
     1321 cgggcggccc tgatcgcggg tctggtggtt ctggctgcgg ccagcctctg gatactgcgg
     1381 cgaccgcgac gggaggacct gcccggctcg cccttcttct gggtcgcgcg cgcgacgaga
     1441 tccggatcgg gcggcgtct
//
LOCUS       DAOQMF010000581         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_71371_length_1458_cov_2.512473, whole genome shotgun sequence.
ACCESSION   DAOQMF010000581 DAOQMF010000000
VERSION     DAOQMF010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71371_length_1458_cov_2.512473"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLV77_09215"
     CDS             complement(<1..496)
                     /locus_tag="PLV77_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC16069.1"
                     /translation="MPVKKLRYLLMVALAAMALGLVACGSDDSSDDSGSSGGTIVKND
                     ANGDTPTLTIGSKNFTEQIVLGEIYAQALEAAGYKVKTDLNLGSETVALAALKKGEIS
                     GYPEYISTALTSFYDTAPEDVPGDVNGAYEMAKNDLAKDGIVALPPTPFASANAVGML
                     TSKAK"
     gene            complement(604..1329)
                     /locus_tag="PLV77_09220"
     CDS             complement(604..1329)
                     /locus_tag="PLV77_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC16070.1"
                     /translation="MPLPALPVGFLSLFGEAFTFVFSGREAETGGVMVGGTGQVLDLA
                     EKQLEVTLLALAVAILLALPVGLWLGHRQKGEFLAVSAGNAGRAIPELALIALMAAAI
                     GVGLVNLVVALAILGIPPILTNTYVGISQVDRASVEAARGMGMTEPAIVRRVELPLAV
                     PTIMGGVRTAAINIIATATIAPLAGILTLGDFIIARNVYGDAGVLAGAILVALMALAL
                     EGTLAGIQWAMTSKGLRQQGRGA"
BASE COUNT          242 a          480 c          490 g          246 t
ORIGIN      
        1 ccttcgcctt cgaggtcagc atgccgaccg cattggcgct ggcgaacggg gtcggcggca
       61 gggcgacgat gccgtccttg gccaggtcgt tcttcgccat ctcgtaggcg ccgttcacgt
      121 cacccggaac atcctccggt gccgtgtcgt aaaacgaggt cagggcggtc gagatgtact
      181 ccgggtagcc cgagatctcg cccttcttca gtgcggcgag ggcgaccgtc tccgaaccga
      241 ggttgaggtc ggtcttgacc ttgtagccgg cggcctcgag cgcctgggcg tagatctcgc
      301 cgagaacgat ctgctcggtg aagttcttcg agccgatggt cagggtcggg gtgtctccgt
      361 tggcgtcgtt cttgacgatc gtgccgccgg agctgcccga gtcatcgctc gaatcatccg
      421 atccgcatgc gaccaggccc agggccatcg ccgctagcgc gaccatcagc aggtagcgca
      481 gtttcttgac aggcatatgt cgtttctctc cttgactggg gttggactga gtgggtctct
      541 ccctgagacc gtatccgccc gggcgggcgt tcaagcaagt ttggcttcag gaaagcttca
      601 gcttcaggcc ccgcggccct gctgacgcag gcccttcgag gtcatcgccc actggatccc
      661 ggccagagtc ccctccaggg ccagggccat cagggcgacc aggatcgccc cggccagaac
      721 cccggcgtcg ccgtagacgt tgcgggcgat gatgaagtca ccgagggtca agatcccggc
      781 cagcggggcg atcgtcgcgg tggcaatgat gttgatcgcc gcggtcctga ccccgcccat
      841 gatggtcggc accgccagcg gcagctcgac ccggcggacg atcgccggct cggtcatgcc
      901 catgccgcgg gccgcctcta ccgaggcccg gtcgacctgg ctgatcccga cgtaggtgtt
      961 ggtcaggatc gggggaatgc cgaggatggc cagggcaacg accaggttga ccaggccgac
     1021 cccgatcgcc gccgccatca gggcgatcag cgcgagttcg gggatcgccc ggccggcgtt
     1081 gccggcggac accgcgagga attcgccctt ctggcggtgg ccgagccaga ggccgaccgg
     1141 cagcgccaga aggatcgcca cggcgagggc cagcagggtc acctcgagct gcttttcagc
     1201 gaggtcgagg acctgcccgg ttccacccac catcacgccc ccggtttcgg cttcccggcc
     1261 ggagaagacg aaggtgaagg cttccccgaa gaggctgaga aagccgaccg gaagggcggg
     1321 caagggcatc aggctgtcac ccgcttccgc ttccagggcg cgagcagctt gccgaccaaa
     1381 agcagcacgc cgtcgaagac gatcgccatg atcaccgcga tcgaaccggc gacgattatc
     1441 cccgtcttga aggtgatg
//
LOCUS       DAOQMF010000582         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_71440_length_1457_cov_2.941512, whole genome shotgun sequence.
ACCESSION   DAOQMF010000582 DAOQMF010000000
VERSION     DAOQMF010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71440_length_1457_cov_2.941512"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLV77_09225"
     CDS             complement(<1..331)
                     /locus_tag="PLV77_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16071.1"
                     /translation="MSTSGGRDNHHNRWPASLIALFAAALLLAAFLIAADRAAAIPPE
                     QLPAATIVFENGGRIVSIKADGTGRKVLTPRRAKTMIETGIRDSDPAVSPDGQTVLFS
                     RDDDSSGS"
     gene            complement(493..566)
                     /locus_tag="PLV77_09230"
     tRNA            complement(493..566)
                     /locus_tag="PLV77_09230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(530..532),aa:Pro,seq:cgg)
     gene            complement(618..1337)
                     /locus_tag="PLV77_09235"
     CDS             complement(618..1337)
                     /locus_tag="PLV77_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16072.1"
                     /translation="MSSAFRQRRSGQFRTLIILVVAIGALLIALVGMAIQKPDNEYEN
                     VQGKQDMRQIFGGVRQLDDRLGSDDAPAQMQVFLDAQSSTYKDQFLDTIPALVSGPVR
                     DGTLKLLYRNRSLTRNATERSFYGIEAAMKQGYGWQYAYLMFRNQDLAKEQGIDDDFL
                     TTLAESIDNLEIEQWKQDYQDGLEEGSAMNADLQAQDQIAIDLKIRDAPAAVVNGPSG
                     TEILQDAPDLAEMQAAIGEVQ"
BASE COUNT          237 a          465 c          472 g          283 t
ORIGIN      
        1 ccgatccgga tgaatcatcg tcccgcgaga agagcacggt ctgcccgtcc ggagagacgg
       61 ccggatccga atcccggatc ccggtctcga tcatcgtctt tgcccgccgc ggggtcagga
      121 cctttcgccc ggtcccgtcg gccttgatcg agacgatccg gccgccgttc tcgaagacga
      181 tcgtggccgc gggcagctgc tccggcggga tggccgcggc ccggtctgcc gcgatgagga
      241 aagcggcaag cagcagcgcc gcggcgaaaa gcgcgatgag cgatgcgggc caccggttgt
      301 ggtggttatc cctgccccct gatgtcgaca tcctgcgact catctttctg tttcccggat
      361 ccgttccggt ttccctgtag aagcagggat tctaccgtcc ggtgacttga aactgttacg
      421 tgggtgtgtc ctcgcccgtc acggtaaagc tgacggatga cacaccccgt aacacggtcg
      481 gattgagaag tatcggggcg cccggatttg aaccggggac ctcaccgacc cgaacggtgc
      541 gcgctaccag gctgcgccac gccccgagga agaatgagcc tacagaaatt gcggcccgga
      601 acccgtcccg gcggcggcta ctggacctcg ccgatcgccg cctgcatctc ggccaggtcg
      661 ggcgcgtcct gaaggatctc ggtcccggac gggccgttga ccaccgcagc gggggcgtcg
      721 cggatcttca ggtcgatcgc gatctggtcc tgggcctgga gatcggcgtt catggcgctg
      781 ccttcctcga gaccatcctg gtaatcctgc ttccactgtt cgatctcgag gttgtcgatc
      841 gactccgcca gcgtggtcag gaagtcgtcg tctatgccct gctccttggc gagatcctgg
      901 ttgcggaaca tcaggtaggc gtactgccag ccgtagccct gcttcatcgc tgcctcgatc
      961 ccgtagaagc tgcgctccgt cgcattgcgg gtgagcgaac ggttgcggta gagaagcttg
     1021 agggtgccgt cccggaccgg cccgctgacc agggcgggaa tcgtgtcgag gaactggtcc
     1081 ttgtaggtcg agctctgggc gtcgagaaag acctgcatct gggccggggc gtcatccgag
     1141 ccgagccggt cgtcgagctg gcgcaccccg ccgaagatct ggcgcatgtc ctgcttgccc
     1201 tggacgttct cgtattcgtt gtcgggtttc tggatcgcca tgccgaccag ggcgatcagc
     1261 agggcaccga tcgccaccac gaggatgatc agcgtccgga actggccgga acggcgctgc
     1321 ctgaaggcgc tgctcatctc agccggttac cgatttgtag taccgcaccg caagaccggt
     1381 gatcagggtt ttgaaggcct gttcgagctc catgtcctcc tcttcggaga aacggaccag
     1441 accgcgctcc tcgatcg
//
LOCUS       DAOQMF010000583         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_71604_length_1455_cov_3.291429, whole genome shotgun sequence.
ACCESSION   DAOQMF010000583 DAOQMF010000000
VERSION     DAOQMF010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71604_length_1455_cov_3.291429"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..877
                     /locus_tag="PLV77_09240"
     CDS             41..877
                     /locus_tag="PLV77_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC16073.1"
                     /translation="MSGAVATASGSLAGPDSPGPGHSTLPIQVSAMASRSVKRLIRQP
                     ALVIPSVIFPLFLLAVNAGGLNAATHIPGFPTDSYLTFAIGFTFIQAAIFSTMGAGQS
                     LAQDIENGFFSRLVLTPMRPTALMAGQLAGLVLLGQFQALLYLSVGLAAGAHFEAGLA
                     GYFVLAALVSIISIGFGGIGLIMAIRTGSGEAVQGMFPLMFVLLFFSSMTLPRNLIEQ
                     DWYRFLATINPVSYLIEAIRSLFIYGWDTEALALGFGVATVVAVVSIIFAAHSLQGRM
                     LK"
     gene            874..>1455
                     /locus_tag="PLV77_09245"
     CDS             874..>1455
                     /locus_tag="PLV77_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16074.1"
                     /translation="MIPDRKYFSAGWAVAWRYLHNLYTKPAVFVPGLIFPLFFLAAFA
                     GGLSAVGDTPKFDYYDFTAFQFCFILIQASALAGVFVGFSIAGDFEQGMGRRMMLATS
                     RRSSILLGYAIGAALRLIVVWVIITGVSLLAGMEVRGSGYDLVGMYSLGLLANVVALL
                     FSAGIAFRFRSLQAGPLMQMPVFIALFLAPVYVP"
BASE COUNT          182 a          517 c          454 g          302 t
ORIGIN      
        1 ccgagatgcg gttgcccgag gtcgaagcgg caggttcctc gtgagcggag ccgttgcaac
       61 cgcgagcggg agcctggccg ggccggacag ccccggcccc gggcattcga cgctgccgat
      121 ccaggtctcg gcgatggcga gccggtcggt caagcggctg atccgccagc ccgccctggt
      181 aattcccagc gtgatcttcc ccctcttcct gctcgccgtg aacgcgggcg ggctcaacgc
      241 ggcgacccac ataccgggct tcccgaccga ttcctacctg accttcgcga tcggcttcac
      301 cttcatccag gcggcgatct tctcgaccat gggggcgggc cagagcctcg cccaggacat
      361 cgagaacggc ttcttcagcc gcctggtgct gaccccgatg cggcccaccg cgctcatggc
      421 cggccagctc gccggtctgg tcctgctcgg gcagttccag gcgctgctct acctctcggt
      481 cggtctcgcc gccggggcgc atttcgaggc cggtctggcc ggctacttcg tgctggccgc
      541 cctggtctcg atcatctcga tcggcttcgg cgggatcggc ctgatcatgg cgatccgcac
      601 cggctccggc gaggcggtcc agggcatgtt cccgctcatg ttcgtgctgc tcttcttcag
      661 ctcgatgacc ctgccgcgca acctgatcga gcaggactgg taccgcttcc tcgccacgat
      721 caacccggtc agctacctga tcgaggcgat ccgcagcctg ttcatctacg gctgggacac
      781 cgaagccctg gcgctcggct ttggcgtggc gaccgtggtc gcggtggttt cgatcatctt
      841 cgcggcccac tcgcttcagg ggaggatgct caagtgatcc ccgatcgcaa gtacttctcc
      901 gcgggctggg cggtcgcctg gcgctacctg cacaacctct acaccaagcc ggccgtgttc
      961 gtgcccgggc tgatcttccc gctgttcttc ctggcagcct ttgcgggcgg actctccgcg
     1021 gtcggcgaca cgccgaaatt cgactattac gacttcaccg ccttccagtt ctgcttcatc
     1081 ctgatccagg cttcggccct ggccggggtc ttcgtcggtt tctcgatcgc cggtgacttc
     1141 gagcagggga tgggcagacg gatgatgctg gccacatcgc ggcgctcctc gatcctgctc
     1201 ggatacgcga tcggcgccgc cctgagactg atcgtggtct gggtgatcat caccggcgtc
     1261 tccctgctgg ccgggatgga ggttcgcggc agcggctacg acctggtcgg catgtactcg
     1321 ctcggcttgc tcgccaacgt ggtcgccctg ctcttctcgg ccgggatcgc cttccgtttc
     1381 cgttcgctcc aggccggacc gctgatgcag atgccggtct tcatcgcgct cttcctcgcg
     1441 ccggtctacg tgccg
//
LOCUS       DAOQMF010000584         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_71618_length_1455_cov_2.977857, whole genome shotgun sequence.
ACCESSION   DAOQMF010000584 DAOQMF010000000
VERSION     DAOQMF010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71618_length_1455_cov_2.977857"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLV77_09250"
     CDS             complement(<1..264)
                     /locus_tag="PLV77_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC16075.1"
                     /translation="MSDYAVVNPATGETVKEYPTISDAGLQEAIASAEDAFRDWSKNS
                     SIEERAAAVRRVGELHVERREELADIIIREMGKHVARPIIFPLS"
     gene            406..582
                     /locus_tag="PLV77_09255"
     CDS             406..582
                     /locus_tag="PLV77_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNC16076.1"
                     /translation="MTDHKTDEAKGRVKEAAGAITDDDELKNEGKADRAASSVKEKVE
                     DAVDTVKEKLAGKD"
     gene            618..890
                     /locus_tag="PLV77_09260"
     CDS             618..890
                     /locus_tag="PLV77_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HNC16077.1"
                     /translation="MCRNIRTLYNFEPEATDEEVRAASLQYVRKISGFNKPSRANEEA
                     FDRAVEEIAAVSARLLDSLETSAPPKDRETEERKRRERAAKRFAAA"
     gene            887..>1455
                     /locus_tag="PLV77_09265"
     CDS             887..>1455
                     /locus_tag="PLV77_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC16078.1"
                     /translation="MSAADLRDWRDRGRMVPATPLGHEAFLIDEGNPEAPPESTVVLL
                     HGFPESSYSYSGAFPLVSGAFDRVVSFDFIGFGFSAKPADGFAYSLIEQADVALRLIR
                     GSGITGCHFVAHDMGDSVMTEILARLNENRPDWFRDGALSVSFTNGSMVLGDAKLRVG
                     QRALVSPAGRMLSRIMDSPKIFEKQVVSA"
BASE COUNT          278 a          449 c          487 g          241 t
ORIGIN      
        1 ggagagcggg aagatgatcg gacgggccac atgcttgccc atctcgcgga tgatgatgtc
       61 ggccagttcc tcacggcgct cgacgtgaag ctcgccgacc cggcgcaccg ccgcggcccg
      121 ttcctcgatc gaactgttct tcgaccagtc ccggaaggca tcctcggcgg aagcgatcgc
      181 ttcctggagg ccggcgtccg agatcgtcgg atactccttc acggtttcgc ctgttgccgg
      241 gttcacaact gcgtagtcgc tcatgaatct ccaatcctcg atttgcgcct ttggctgaca
      301 cctgaaggtg tctcaccgca gactattggt tggccgtttc gggtgaatcg gattggagcg
      361 cgagcaccgg ggtactacct ggttgaaacc cagaaggagg tagcaatgac cgatcacaag
      421 acagacgaag ccaagggccg cgtgaaggaa gccgcgggag caatcaccga cgacgatgag
      481 ctcaagaacg aaggcaaggc cgaccgggcc gcttcgtcgg tcaaggagaa ggtggaagac
      541 gcggtggaca cggtcaagga gaaactggcc ggcaaggact gacccgacgc ccccgcggcg
      601 gggcgtaaga tcggggcatg tgccggaata tcaggaccct ttacaacttc gaacccgagg
      661 cgactgacga agaagtgcgg gccgcctccc tccagtacgt gaggaagatc agcgggttca
      721 acaaaccctc cagggccaac gaggaagcgt tcgaccgggc ggtcgaagag atcgccgccg
      781 tctcggcgag gctgctcgat tcgctcgaga cttccgcacc gcccaaggac cgcgagaccg
      841 aggagcggaa gcggcgggag cgggccgcaa aacggttcgc cgcagcgtga gcgccgccga
      901 cctgcgggac tggcgtgacc gtggccgaat ggtcccggcg acgccactgg gccacgaggc
      961 cttcctgatc gatgagggga acccggaagc cccgcccgaa tcgaccgtgg tgctgctgca
     1021 cggcttcccg gaatcctcgt actcgtattc gggtgccttc ccgctggtct ccggggcctt
     1081 cgatcgggtc gtctcgttcg acttcatcgg cttcggcttt tcggccaagc cggctgacgg
     1141 atttgcctat tcgctgatcg agcaggccga tgtcgccctg cgcctgatcc gcgggtcggg
     1201 catcaccggc tgccacttcg tggcgcatga catgggggac tcggtgatga ccgagatcct
     1261 tgcccgcctg aacgagaacc ggccggactg gttccgggac ggggcgctca gcgtcagctt
     1321 caccaacggc agcatggtgc tgggagacgc caaactgcgg gtcgggcaga gggcactggt
     1381 ctcgccggcg gggcgaatgc tctcccggat catggacagc ccgaagatct tcgagaagca
     1441 ggtcgtaagc gccca
//
LOCUS       DAOQMF010000585         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_71899_length_1452_cov_3.751611, whole genome shotgun sequence.
ACCESSION   DAOQMF010000585 DAOQMF010000000
VERSION     DAOQMF010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71899_length_1452_cov_3.751611"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..695)
                     /locus_tag="PLV77_09270"
     CDS             complement(57..695)
                     /locus_tag="PLV77_09270"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNC16079.1"
                     /translation="MKVKVCGITNRDDAEAAVEHGAWAIGLIHHKESPRFVKASVAAG
                     IGEEFRRRCEVVGVFVNPTIERVVQAAENAQLTMIQLSGNEGANFCSEVNRRTGLKVI
                     KAFHISSQADVKASTAYRFTDFHLFDTGRKGVYGGTGETFDWDLLVSYHSPIPTILAG
                     GLNPDNVTEAIRIVRPDAVDVASGVEREPGIKDRGKLASFFQAARYTPVAGA"
     gene            complement(729..>1452)
                     /locus_tag="PLV77_09275"
     CDS             complement(729..>1452)
                     /locus_tag="PLV77_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC16080.1"
                     /translation="MKPLALGDSTDVKVGDPVVAIGNPFGLDRTVTTGIVSALQREIS
                     SLTNYAISNVIQTDAAINPGNSGGPLIDANGNVIGVNSQIATGSGSSTSGNVGIGFAI
                     PSNTVKDVVGQLIEDGKAEHAYLGISGATINEQLANALNLGTDTGVLVQEAPKDGPAA
                     QAGIKAGDTAVTIQGQRVMAGGDVITKVNGTDLGSMEDLIDAVNGAKVGDEMELEVER
                     DGQTSTVTVKLGTRPDDTQPSK"
BASE COUNT          218 a          463 c          473 g          298 t
ORIGIN      
        1 gtaggggccg aagcggcccg gcagctcgcc ggccgtggac tccgcgaaat ggccgctcat
       61 gcgcctgcca ccggggtgta tcgggctgcc tgaaagaagc tggccagctt gccgcggtcc
      121 ttgatccccg gctcgcgctc gacccctgaa gcaacgtcca ccgcgtcggg ccggacgatc
      181 cggatcgcct cggtcacgtt gtcggggttc agccccccgg ccagaatggt cgggatcggc
      241 gagtggtagc tgaccaggag gtcccagtcg aaagtctctc ccgtgccgcc gtaaacgcct
      301 ttgcggccgg tgtcgaaaag gtggaaatcg gtgaagcggt aggcggtgct ggccttgaca
      361 tccgcctggc tggagatgtg aaaagccttg atcactttca ggccggtgcg acggttcact
      421 tcactgcaga agttcgcgcc ctcgttgccg ctcagctgga tcatcgtcag ctgcgcgttc
      481 tcggccgcct ggaccaccct ttcgatggtc gggttgacga aaacgccgac cacttcgcag
      541 cggcgacgga actcctcgcc gatgccggcc gcaaccgacg ccttgacgaa ccgggggctc
      601 tccttgtgat ggatcagccc gatcgcccag gcgccgtgtt ccacggcggc ttcggcgtca
      661 tcccgattgg ttattccaca gacctttacc ttcaccagtc aagggtatga caaccgcccg
      721 tccggccgtc atttcgacgg ctgggtgtca tcgggccggg tgccgagctt gaccgtaacc
      781 gtgctggtct gcccatcgcg ttcgacttcc agttccatct cgtcaccgac ctttgccccg
      841 ttgacggcat cgatcagatc ttccatcgaa ccgaggtccg tgccgttgac cttggtgatt
      901 acgtcacctc cggccatcac ccgctggccc tggatcgtga ccgcggtgtc accggccttg
      961 attccggcct gggcagcggg gccgtccttc ggcgcttcct gaaccaggac gccggtgtcg
     1021 gttccgaggt tgagcgcgtt ggcaagctgc tcgttgatcg tcgcgccgct gattccgagg
     1081 taggcgtgtt cggccttgcc gtcctcgatc agctggccga caacgtcctt gacggtgttc
     1141 gagggaatcg cgaaaccgat gccgacgttg ccgctggtgc tggagccgga accggtggcg
     1201 atctgcgagt tgactccgat cacgttgccg ttggcgtcga tcagcggtcc gccggagttg
     1261 ccggggttga tcgcggcgtc ggtctggatc acgttcgaaa tcgcgtagtt tgtgaggctc
     1321 gatatctccc gctgaagggc ggagacgatg ccggtggtga ccgtgcggtc gaggccgaag
     1381 gggttgccga tcgcgacgac cgggtcaccg accttcacgt cggttgagtc accgagtgcg
     1441 agcggcttca tt
//
LOCUS       DAOQMF010000586         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_72191_length_1449_cov_3.986370, whole genome shotgun sequence.
ACCESSION   DAOQMF010000586 DAOQMF010000000
VERSION     DAOQMF010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72191_length_1449_cov_3.986370"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..436)
                     /gene="purS"
                     /locus_tag="PLV77_09280"
     CDS             complement(206..436)
                     /gene="purS"
                     /locus_tag="PLV77_09280"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNC16081.1"
                     /translation="MIQARVLIRPKEGILDAQGKAVEGALPALGFSGIEHVRIGRIVE
                     LEADSTDQLEELCEKLLANPLIEDYEIETVSG"
     gene            complement(433..1284)
                     /locus_tag="PLV77_09285"
     CDS             complement(433..1284)
                     /locus_tag="PLV77_09285"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNC16082.1"
                     /translation="MANSIEDLKLVNSGKVREIYEIEDDLLLVASDRISIYDVIMPTA
                     IPDKGKVLTQMSNFWFENTTDIVPNHFLSNDVPEEVAGRAVRVRKLEMYPVECVVRGY
                     LSGSGWKEYQETGAVCGVGLPDGLQESDKLPRPIFTPATKAELGDHDENVDFDRAAEI
                     VGDRHLMEELRRISIEIYEYGANHAAEKGIILADTKFEFGSYAGAEIVLGDEVLTPDS
                     SRYWPADQYEPGKSQPSFDKQYVRDWANAAGWDHNPPAPELPEDVVAQTQAKYREAYE
                     RITGREL"
BASE COUNT          253 a          474 c          443 g          279 t
ORIGIN      
        1 gcccccgccc gaaggtagtc cccgtaagag aaaccaccag gcaccacaat cccgtcgacg
       61 tcagacagat ccgtctcctc atgccaaacc aaccgaggca acgagccgga cccgccctca
      121 gagcccaagg ctgtcgcagc gatctggcag gcgttgagcg cgtcccgctc gtcgcaggac
      181 ccggggaact gaagcacacc ccacttcatc cgctgacggt ctcgatctcg taatcctcga
      241 tcagcgggtt ggcgaggagc ttctcgcaaa gctcctcgag ctgatcggtg ctgtccgctt
      301 cgagttcgac gatccggccg atcctgacgt gttcgatccc ggagaaacca agcgccggca
      361 gcgccccttc gaccgccttg ccctgggcat cgaggatgcc ctccttgggc cggatcagaa
      421 cgcgggcctg aatcaaagtt cccttccggt gatccgctcg taggcctcgc ggtacttggc
      481 ctgggtctgg gcgaccacgt cttccggcag ctccggggcc ggcgggttgt gatcccagcc
      541 ggcggcgttg gcccagtcac ggacgtactg cttgtcaaag gaaggctgcg acttgccggg
      601 ctcgtactgg tcggccggcc agtagcgcga agagtcggga gtcagaacct cgtcaccgag
      661 cacgatctcg gccccggcgt agctgccgaa ctcgaacttg gtatcggcga ggatgatccc
      721 cttttcggcc gcgtggtttg cgccgtattc gtagatctcg atcgagatcc ggcgcagttc
      781 ctccatcagg tggcggtcac cgacgatctc ggcggcccga tcgaagtcca cgttctcgtc
      841 gtggtcgccg agttcggcct tggtcgccgg ggtgaagatc ggccggggca acttgtcgga
      901 ttcctgcagg ccgtcgggca gcccgacccc gcagaccgcg ccggtctcct ggtactcctt
      961 ccagccggaa ccggagaggt agccgcgaac cacgcactcg accgggtaca tctcgagctt
     1021 gcgcacccgg accgcccgcc cggcgacttc ctccggcacg tcattcgaga ggaagtggtt
     1081 cggcacgatg tccgtggtgt tctcgaacca gaagttggac atctgggtca gaaccttgcc
     1141 cttgtcgggt atcgcggtcg gcatgatcac gtcgtagatg gagatgcggt cggaggcgac
     1201 gaggaggagg tcatcctcga tctcgtagat ctcgcggacc tttccggaat tgacaagctt
     1261 gaggtcttca atcgaattcg ccattaaagg acaaactacc caatcgacaa gccggaaagc
     1321 cggactgcgt ataacctccg caaatgcgca cttttctggt tgcttccgtg agcctgatcg
     1381 cgtcggcttt cgccctttcg ggagtcgcgg cggcggctga agtgcctccc tgtaccggct
     1441 ctttcagcc
//
LOCUS       DAOQMF010000587         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_72209_length_1449_cov_3.347202, whole genome shotgun sequence.
ACCESSION   DAOQMF010000587 DAOQMF010000000
VERSION     DAOQMF010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72209_length_1449_cov_3.347202"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLV77_09290"
     CDS             complement(<1..474)
                     /locus_tag="PLV77_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC16083.1"
                     /translation="MRPGPVQSTAEDNLLYGAVHLNVTDLERSLVFWRDLIGMVELGD
                     GESGAVRLGVKGEPDEPGKELVVLHPGATRPVQRGYSALYHLAIHMPSEAEFARILAR
                     LFAARYPNAPTDHVTHWATYLDDPDGINVEIAFETIDRVGEIDIVGGRPLIIDSEG"
     gene            519..>1449
                     /locus_tag="PLV77_09295"
     CDS             519..>1449
                     /locus_tag="PLV77_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16084.1"
                     /translation="MRNRAPVLVLIGCLILSAALIIHAGRDLTFFFDEWQLLLASDPL
                     SLSTLLEPHNSQPSFMTSSIYGLLLETVGMSSQLPYRLVAVAASIACVTLLYLLVRRR
                     TDPWLALVMILPVLGLGVAWEALLLALSMNFLIGIATGLGALLALESRSRRGDLLACF
                     LLCCSLTCGGVGLAFLAGACVDVLVRRDARRAWIPAVPVLLYGLWSFAFGDEDSPASA
                     DNALGLPDYLYKTSAAGIRSVTGLAADLFPARPAFYLSVLIFALLLAAVVWRLFFAKR
                     PYSDRVLVAGGALLAFWVLGGLALGEGRAPDASR"
BASE COUNT          178 a          496 c          468 g          307 t
ORIGIN      
        1 gccttcggaa tcgatgatca gcggccggcc gccgacgatg tcgatctcgc cgacccggtc
       61 gatcgtctcg aaggcgatct cgacattgat cccgtccgga tcatcgagat aggtcgccca
      121 gtgggtgacg tggtcggtcg gggcgttggg gtagcgggca gcgaagaggc gagcaaggat
      181 gcgggcgaac tccgcctccg agggcatgtg gatcgccagg tggtagaggg ccgagtagcc
      241 ccgctgaacg ggccgggtgg ctcccggatg aagcacgacg agttcctttc cgggctcgtc
      301 cggctcgccc ttgactccca gccgcacggc cccactctcc ccgtctccga gctcgaccat
      361 tccgatcagg tcccgccaga agaccagcga cctttcgagg tcggtcacgt tcaggtggac
      421 ggctccgtaa aggagattgt cttcggcggt gctctgaacc ggtccgggtc gcatgcccca
      481 actatggcag cccccggagt ccggttgaat acctgctctt gcgcaaccgg gcaccagttc
      541 tcgtcctgat cggatgtctg attctctcgg ccgccctgat catccatgca gggcgcgacc
      601 tgaccttctt ctttgacgag tggcagctgc ttctggcctc cgatccgctg tcgctttcga
      661 ccctgctcga gccccacaac agccagccct ccttcatgac ttcgtcgatc tacgggctgt
      721 tgctcgagac ggtcgggatg tcatcccagc tcccgtaccg gttggtcgcg gtggccgctt
      781 cgatcgcctg tgtgacgttg ctatatctgc tggtgcgccg ccgtaccgac ccttggctgg
      841 ccctggtcat gatccttccg gtgctgggac tcggggttgc ctgggaggct ctgcttctgg
      901 ccctctcgat gaacttcctg atcggaatcg cgaccggtct cggtgccttg ctggcgctgg
      961 agtcccgcag ccggcgcggc gatctgctcg cctgctttct gctctgttgc tcgcttacct
     1021 gcggcggggt cggccttgcc ttcctggccg gagcctgcgt tgacgtgctg gtgcgccggg
     1081 acgctcgcag ggcctggatc ccggcggtcc cggtcctgct ctacgggctc tggagcttcg
     1141 ccttcggaga tgaggattcc ccggccagcg cggacaacgc cctcggcctg ccggactatc
     1201 tctacaagac ctccgcggcc gggatccgct cggtcaccgg ccttgccgcg gacctgttcc
     1261 cggcccgacc ggccttctac ctgtcggtgc tcatcttcgc cctgcttctg gcggcggtcg
     1321 tctggcggct cttcttcgcc aaacggcctt acagcgaccg ggtcctggtg gcgggcgggg
     1381 cgctgctcgc cttctgggtg ctcggtggtc tcgccctcgg agaaggccgg gccccggacg
     1441 cgagccgct
//
LOCUS       DAOQMF010000588         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_72210_length_1449_cov_3.331420, whole genome shotgun sequence.
ACCESSION   DAOQMF010000588 DAOQMF010000000
VERSION     DAOQMF010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72210_length_1449_cov_3.331420"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..590)
                     /locus_tag="PLV77_09300"
     CDS             complement(33..590)
                     /locus_tag="PLV77_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16085.1"
                     /translation="MESRISSWLRCYRCWSRNLEVQVHYDAIRPIDPETGIPTEGDDE
                     IQESVVQCLDCMHDQPHLTFERDRVVPIEDRWERMVAGTPWVASCTVTVEADQVEACA
                     GPEAVESLTFGAFGDAGTREFFTHVRFHKHHEDQIAVHLLVELYARNPEEASEVLTGA
                     ARGTIEITSLAEESRPPAHASGDTH"
     gene            complement(646..1425)
                     /locus_tag="PLV77_09305"
     CDS             complement(646..1425)
                     /locus_tag="PLV77_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC16086.1"
                     /translation="MATKSQQKRKELRDKRMQAEQAAQSADKRQNLAKILGIAAFLVV
                     VGVVVAVVAISSSGGGDEPKGSGDIDKLLAGIPQNGTTLGEANSKVTLYEFGDLQCPI
                     CEQYSEDATPDIINNIVRPGKAKWNFKQWAILGPDSTLAAKAALAAAEQNRYWEFIEN
                     FYANQGIENSGYVTDDFLTEIANDAGVPDIDKWNADRESAAIAKQVEQTDAQASSLGF
                     SGTPSFGVQVGNGDIEPVQTTSSTAAAGEIAKAIEKAQSGN"
BASE COUNT          211 a          458 c          478 g          302 t
ORIGIN      
        1 ggtggagggc gggtcggacc ccgcccgact tcttaatggg tgtcgcccga ggcgtgggcg
       61 gggggccggg actcctcggc cagtgaggtg atctcgatcg ttccccgggc ggcgccggtc
      121 agcacttcgc tcgcctcctc cgggttgcgg gcgtagagct cgaccagcag gtgtaccgcg
      181 atctggtctt cgtggtgctt gtggaagcgg acgtgggtga agaactcacg ggttccggca
      241 tcgccgaagg cgccgaaggt cagcgattca accgcttccg gtcccgcgca ggcctcgacc
      301 tgatcggcct cgaccgtgac cgtgcatgag gcgacccagg gcgtcccggc caccatgcgt
      361 tcccagcgat cctcgatcgg taccacccgg tcccgctcga aggtcaggtg cggctggtca
      421 tgcatgcagt ccaggcactg gaccaccgat tcctggatct cgtcatcgcc ctcggtcggg
      481 ataccggtct ccgggtcgat cggccggatc gcgtcgtagt ggacctgaac ctcgaggttg
      541 cgtgaccagc accggtagca cctgagccat gaagagatac gggactccat gctcatatct
      601 ttgcgcctcg gagacctcgg cggggcatgt ggctcgctat gtgggtcagt tacccgactg
      661 cgccttctcg atcgccttgg cgatctcgcc ggctgccgca gttgaactgg tggtctggac
      721 cggctcgatg tcaccgttgc cgacctggac cccaaacgac ggggtgccgc tgaagccgag
      781 gctcgaggcc tgcgcgtcgg tctgctcgac ctgcttggcg atcgcggcgg actcccgatc
      841 ggcattccac ttgtcgatgt ccggaacgcc ggcgtcgttg gcgatttcgg tgaggaagtc
      901 gtcggtcaca taaccggaat tctcgatgcc ctgattggcg tagaagttct cgatgaattc
      961 ccagtaacgg ttctgttccg cagcggcgag cgcagccttg gcagcgaggg tcgaatccgg
     1021 gccgagaatc gcccactgct tgaagttcca cttggccttg ccgggccgga cgatgttgtt
     1081 gatgatgtcc ggggtggcgt cctccgagta ctgctcgcag atcgggcact gcaggtcacc
     1141 gaactcgtac agcgtcacct tgctgtttgc ttcgccgagc gtggtgccgt tctgcgggat
     1201 tccggccagc agcttgtcga tgtctcccga tcccttcggc tcgtcgccgc ctccgctgga
     1261 gctgatcgcg acgaccgcca cgaccacgcc gaccacgacc agaaaggccg cgatcccgag
     1321 gatctttgcg aggttctgac gcttgtcggc gctctgcgca gcctgctcgg cctgcatgcg
     1381 cttgtcgcgg agttcttttc tcttctgctg cgatttggtg gccattggcg gggatgctac
     1441 taagaggcg
//
LOCUS       DAOQMF010000589         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_72315_length_1448_cov_3.300072, whole genome shotgun sequence.
ACCESSION   DAOQMF010000589 DAOQMF010000000
VERSION     DAOQMF010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72315_length_1448_cov_3.300072"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PLV77_09310"
     CDS             complement(<1..816)
                     /locus_tag="PLV77_09310"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNC16087.1"
                     /translation="MSNAQFEKETDKKGAFVRQTSAFRERVTADGSSGFPAEPGRYHL
                     YVCYACPWASRTIIFRTLKGLEDVISMTAVDPVRDDQGWKFFADDPDPVNGYEYLLEA
                     YLRTDPDFSDRVTVPVLWDKETNRAVNNESSEIIRMLNTEFNQWAKNPDLDLYPEPLR
                     AEIDELNDRIYNSINNGVYRAGFATTQEAYEEAFVELFEALDVMNDRLGESRFLTGGE
                     ITEADWRFFVTLIRFDAVYVGHFKCNLRRIADYDNLAGYLRDLYQQPGIRETVN"
     gene            945..>1448
                     /locus_tag="PLV77_09315"
     CDS             945..>1448
                     /locus_tag="PLV77_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16088.1"
                     /translation="MSNARFSSLSRIHLLGLGLLALVTLALLAAPSKSRAADQPFKID
                     FDQSSMQIGLLSDLPLGDLATTGSLEGTIDDEGNVTIPKGNFILPEIGITDPIAIKVF
                     MGVESDATGTYDADTGELVLNAKAGVWFSVDLQALLSTLGTLGVQLPAGSGIIETILC
                     QVTDLTCG"
BASE COUNT          248 a          451 c          463 g          286 t
ORIGIN      
        1 gttgaccgtc tcgcggatgc cgggctgctg gtagaggtca cgcaggtagc cggcgagatt
       61 gtcgtagtcg gcgatccgtc gcaggttgca cttgaagtgg ccgacgtaga cggcgtcaaa
      121 gcggatcagg gtgacgaaga agcgccagtc cgcctcggtg atctcgccgc cggtcaggaa
      181 gcggctctcc cccagccggt cgttcatcac gtcgagcgcc tcgaagagct ccacgaaggc
      241 ctcctcgtat gcctcctgcg ttgtggcgaa gccggcccgg tagaccccgt tgttgatcga
      301 gttgtagatc cggtcgttca gttcgtcgat ttccgcccgg agcggttcgg ggtaaaggtc
      361 gaggtcgggg ttcttcgccc actggttgaa ctcggtgttg agcatgcgga tgatctcgct
      421 gctctcgttg ttgaccgcgc ggttggtctc cttgtcccag agcacaggca cggtgacccg
      481 gtcgctgaag tcggggtcgg tgcgaaggta ggcctcgagc aggtactcgt agccgttgac
      541 cgggtcggga tcgtcggcga agaacttcca gccctgatcg tcccggactg ggtcgacggc
      601 ggtcatcgag atcacatcct ccagcccttt cagggtgcgg aagatgatcg tccgcgaggc
      661 ccaggggcag gcgtaacaga cgtacaggtg gtacctgcca ggttccgccg gaaagcctga
      721 agagccgtcg gcggttaccc gctccctgaa ggcgctcgtc tggcggacga acgcgccttt
      781 tttgtcggtt tccttctcaa actgtgcatt gctcacgttg ccgactttac ccatcggctc
      841 cggaaaacaa gccgggcgac ggattcgcgt ttatagtgtg atcagcgccg gaaccggtcc
      901 ggctcgtcga tgcccaggga gcgggcggaa acaagggaga agaagtgtcc aacgcgcgtt
      961 tttccagtct ttcgaggatc cacctcctcg gcctcggcct gcttgccctg gtcaccctcg
     1021 ccctgctggc cgccccatcg aagtcccggg ctgcggatca gcctttcaag atcgacttcg
     1081 accagagcag catgcagatc ggcctgctca gcgatctgcc gctcggcgac ctcgcgacca
     1141 ccggctccct cgaagggacg atcgacgacg agggtaacgt cacaatcccc aagggcaact
     1201 tcatcctccc cgagatcggc atcaccgacc cgatcgcgat caaggtgttc atgggtgtcg
     1261 agagcgacgc caccgggact tacgatgccg acaccggtga actggtcctc aacgccaagg
     1321 cgggggtctg gttctcggtc gacctccagg ccctgctctc cacgctcggc acgctgggag
     1381 tccagctgcc ggcgggttcg ggaatcatcg aaacgatcct ttgccaggtg accgacctga
     1441 cctgtggg
//
LOCUS       DAOQMF010000590         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_72320_length_1448_cov_3.046662, whole genome shotgun sequence.
ACCESSION   DAOQMF010000590 DAOQMF010000000
VERSION     DAOQMF010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72320_length_1448_cov_3.046662"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="thrC"
                     /locus_tag="PLV77_09320"
     CDS             <1..1039
                     /gene="thrC"
                     /locus_tag="PLV77_09320"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC16089.1"
                     /translation="AMPQPLIERYRDALPLDPGDPVVSLNEGSTPLVEAPRLSERVGA
                     RVWLKVEGANPTGSFKDRGMTVAVSRAAGRGAKAVICASTGNTAASASAYAVRAGLRG
                     AVIVPEGKIAIGKLAQSLMHGARVVALEGNFDDALTIVRQLAERNPIELVNSVNPYRI
                     EGQKTAAFELIDELGQAPDALAIPVGNAGNVTAYWKGFQERGTAPICYGFQAAGSAPL
                     VDGAPVAHPETVASAIRIGNPARWEEAMNAFNSSRGRVAAVSDEQILDAYRWLAANEG
                     VFCEPASAASVAGILNHGLPTVEGMEKPETVVCVLTGHGLKDPDTALGKAPSVINCPN
                     EIGAVEEAVFD"
     gene            1191..>1448
                     /locus_tag="PLV77_09325"
     CDS             1191..>1448
                     /locus_tag="PLV77_09325"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HNC16090.1"
                     /translation="MKGLLGVLAAAATLLGGLIAAAPASAAFSDIPTSTVSGPIAASA
                     SSYPFLATDIDLDEYGYVEQEFFLEGEGYRYNTPLVNLNTSA"
BASE COUNT          249 a          478 c          472 g          249 t
ORIGIN      
        1 agccatgcca cagccactta tcgagaggta ccgcgacgcc ctgccgctgg atccgggtga
       61 tccggtcgtc agcctgaacg agggctcgac cccgctggtc gaggcgccgc gcctttccga
      121 gcgggtcggg gcccgggtct ggctgaaggt cgaaggtgcg aacccgaccg gctccttcaa
      181 ggatcgcggc atgaccgtgg cggtttcccg ggcggccggg cgtggtgcca aggccgtgat
      241 ctgtgcctcg accggcaaca ccgccgcttc cgcctccgcc tacgcggtgc gggcggggct
      301 gcgcggcgcg gtcatcgtcc ccgaaggcaa gatcgcgatc ggcaagctgg cccaatcgct
      361 catgcacggg gcccgggtgg tcgccctcga aggcaacttc gatgatgccc tgaccatcgt
      421 ccggcagctg gccgaacgga atccgatcga gctggtcaac tcggtcaacc cgtacaggat
      481 cgaaggtcag aagacggccg cgttcgagct catcgacgag cttggccagg ccccggacgc
      541 actggcgatc ccggtcggca acgccggcaa cgtgaccgcc tactggaagg gctttcagga
      601 aaggggaacc gcaccgatct gttacggctt ccaggccgcc ggctcggctc cgctggttga
      661 cggcgccccg gtcgcccacc cggagacggt cgcttcggcg atccggatcg gcaacccggc
      721 ccgctgggaa gaggcgatga acgccttcaa ctcctcgagg ggacgggtcg ccgcggtcag
      781 cgacgagcag atcctcgacg cctaccgctg gcttgccgcc aacgaaggtg tgttctgcga
      841 accggcctcg gccgcttcgg tggccggaat tctcaaccac ggcctgccga ccgtggaggg
      901 aatggagaag cccgagacgg ttgtctgcgt actcaccggc cacggcctca aggatccgga
      961 caccgctctc ggcaaggccc cctcggtgat caactgcccg aacgaaatcg gcgcggtcga
     1021 agaagctgtc ttcgactgag tcctggctct ggatcgaagt tatccgcgcg tggaacgaaa
     1081 aacttcaacc ccgttcctct ccgtagcttg agggccaatc ccgggttatc tcctgtagcg
     1141 tcagccctgc ttcacgggga atcgagggac aaggagattt gatgggtgct ttgaagggac
     1201 tcctgggtgt gcttgcggca gccgccactt tgctgggcgg gctgattgcg gctgccccgg
     1261 cttcggctgc tttcagtgac attccgactt caacggtttc gggaccgatc gctgcgagcg
     1321 cgagctccta ccccttcctg gccaccgaca tcgatctcga cgagtacgga tacgtcgaac
     1381 aggagttctt cctagagggc gaggggtacc gctacaacac cccgctggtc aatctcaaca
     1441 cgagcgct
//
LOCUS       DAOQMF010000591         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_72445_length_1447_cov_2.730603, whole genome shotgun sequence.
ACCESSION   DAOQMF010000591 DAOQMF010000000
VERSION     DAOQMF010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72445_length_1447_cov_2.730603"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..774
                     /locus_tag="PLV77_09330"
     CDS             106..774
                     /locus_tag="PLV77_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16091.1"
                     /translation="MDKAAGPRRLGWLAPVTIGLLLVATVAAFGWSQRLKREPLVIDR
                     VEYRVLGYTPNGESPTVFSPNGDCRRDRMSIHFRTTRSDTADVEIIGLGGRTVRTLAR
                     DRFFKRYREHTLIWNGRKDDGGIAFTSKYRVRVTMHGEDRVLYLPGFIRLHRYAPRGP
                     SGCPKPLKWKYSVKEPGKPARPLTRAEVAKMTRPLTEAEVAKIKAAAKKALGTGGTTG
                     GQGQ"
     gene            771..>1447
                     /locus_tag="PLV77_09335"
     CDS             771..>1447
                     /locus_tag="PLV77_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16092.1"
                     /translation="MIEALAALVAAAALAVAILDQPSMRRSIAMAVAGIMVPVLIAGD
                     QWHSGPITELRNSPAMLVLALLCALAVVALIAWVVDRWPVMLPVLVLVALPFRIPLNV
                     GGEDANLLVPLYLVMGGGVLASLIRARRNDLLPALPTVAPAGLARWLKPLLAAAVVLY
                     ALGLFYTDDRSTGLQNLCFFLIPFGVIFALMAEVEWTRDRLRILLGVVVGLALVCALV
                     GFVEYATR"
BASE COUNT          207 a          511 c          482 g          247 t
ORIGIN      
        1 attgacaaca cggccccgat ggacgccgcc atctcgggac tttcttccgg tgccgacgtg
       61 accgtggtga tcggcaccga ccacggcccc ctgcccgggg agtagatgga caaggccgcc
      121 ggcccccgtc gcctgggatg gctggcgcca gtcacgatcg gcctgctcct ggtggcgacg
      181 gtggccgcct tcggctggtc gcagcggctc aagcgtgagc cgctggtgat cgaccgggtc
      241 gagtaccggg tgctcgggta cacgccgaac ggtgaatcgc cgaccgtctt cagccccaac
      301 ggcgattgcc ggcgggaccg gatgtcgatc cacttccgca cgaccaggtc ggacaccgcc
      361 gacgtcgaga tcatcggcct cggcggcagg accgtccgca ccctggcccg cgaccgcttc
      421 ttcaagcgct accgcgagca cacgctgatc tggaacggtc gcaaggacga cggcgggatc
      481 gcattcacca gcaagtaccg ggtccgggta accatgcacg gcgaggaccg ggtcctctac
      541 ctgcccggct tcatccgcct ccacaggtac gcgccccgcg gaccctccgg ttgcccgaag
      601 ccgctcaagt ggaagtactc ggtcaaggaa ccgggcaaac cggcgagacc cctgacgagg
      661 gccgaggtcg cgaagatgac cagacccctg accgaggccg aggtggcgaa gattaaagcc
      721 gcggcgaaga aggcgctggg caccggcggc accaccggag ggcagggcca gtgatcgagg
      781 cgctcgcagc cctcgtcgcc gcggccgcgc tggcggtcgc gatcctcgac cagccttcga
      841 tgcgtcgctc gatcgcgatg gcggtcgccg gaatcatggt cccggtcctg atcgccggtg
      901 accagtggca ttccggcccg atcaccgaac ttcgaaactc accggcgatg ctggtcctgg
      961 ccctgctctg cgccctggcc gtggtcgccc tgatcgcctg ggtggtcgat cgctggccgg
     1021 tcatgctccc ggtgctggtc ctggtcgcgc tgcccttccg gatcccgctg aacgtcggcg
     1081 gggaggatgc gaacctgctg gtcccgctct atctggtgat gggcggcggg gtgctggctt
     1141 cgctcatccg ggcgcgtcgg aacgacctgc tgcccgcgtt gccgaccgtt gccccggccg
     1201 gtctggcccg ctggctcaaa cccctgctcg ccgccgcagt ggtgctctac gccctcggcc
     1261 tcttctacac ggatgaccgt tcgaccggcc tccagaacct ctgtttcttc ctgattccct
     1321 tcggagtgat cttcgccctg atggctgaag tcgagtggac cagggaccgc ctgcggatcc
     1381 tcttgggcgt ggtcgtcggc ctggccctgg tctgcgccct ggtcggtttc gtcgagtacg
     1441 cgacccg
//
LOCUS       DAOQMF010000592         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_72454_length_1447_cov_2.565374, whole genome shotgun sequence.
ACCESSION   DAOQMF010000592 DAOQMF010000000
VERSION     DAOQMF010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72454_length_1447_cov_2.565374"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PLV77_09340"
     CDS             complement(<1..914)
                     /locus_tag="PLV77_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC16093.1"
                     /translation="MLAVVLISLGLGLAVSACGSSSDEGDSGGSGKEGGEITISQTSQ
                     PDYLDPALSYTVNGWEPMWIVYTPLLTYPHVEGTKGGEVIPGLAEDLPKVSNGGKTYE
                     LKLRKGLKYSDGQPVVASDFTHTIKRVLNLESGGSFFFENIVGADEYIKNGDPEGDIK
                     GITTNDKTGEIKIDLISPDASFSNILATNFAGLVPGDTPFKNLTEDPPPGVGAYVITK
                     SVPNREFVMEKSPNFASFKIPQIPTGHVDKITTEIIKNANQQAQDVLANKLDYMQDPP
                     PADLKPTVEAEASDRYSETTTGSTYYFF"
     gene            complement(1071..>1447)
                     /locus_tag="PLV77_09345"
     CDS             complement(1071..>1447)
                     /locus_tag="PLV77_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16094.1"
                     /translation="KLERALLREDPVEAFRQFVKGWLRYAAEIQPVASMLFAAREADP
                     AAAKAWEDRSRELRAGFRAATARLGKAGMLRDGLSATSAASLSWAICSVPVVEQLALD
                     QEWSKDRVARQLTEAALGAITG"
BASE COUNT          244 a          471 c          435 g          297 t
ORIGIN      
        1 atgaagaagt agtaggtgga accggtggtc gtctccgagt aacggtctga tgcctccgcc
       61 tccacggtcg gcttcaggtc ggccggcggc gggtcctgca tgtagtcgag cttgttggcc
      121 agcacgtcct gcgcctgctg gttggcgttc ttgatgatct cggtcgtgat cttgtcgacg
      181 tgaccggtcg ggatctgcgg aatcttgaag ctggcgaaat tcgggctctt ctccatcacg
      241 aattcgcggt tcggcaccga cttcgtgatc acgtacgcac cgacaccggg cggcggatcc
      301 tcggtcaggt tcttgaaggg tgtgtcacct ggcacgaggc cggcgaagtt ggtcgcgaga
      361 atgttcgaga acgacgcgtc cgggctgatc aggtcgatct tgatttcgcc ggtcttgtcg
      421 ttggtcgtga tgcccttgat gtccccctcg gggtcaccgt tcttgatgta ttcgtcggca
      481 ccgacgatgt tctcgaagaa gaatgaaccg cccgattcga gattcaggac gcgcttgatc
      541 gtgtgggtga agtcgctggc aacgaccggc tggccgtcgg agtacttgag tcccttgcgc
      601 agcttgagct cgtaagtctt gccgccgttg ctgaccttgg gcaggtcctc ggcgagtccg
      661 ggaatgacct cgccgccctt ggtgccttcg acgtgcgggt aagtgagaag cggggtgtag
      721 acgatccaca tcggttccca gccgttgacc gtgtaggaaa gcgccgggtc caggtagtcg
      781 ggctgtgagg tctgggagat cgtgatctct ccaccctcct tgccgcttcc tcccgagtcg
      841 ccttcgtccg agctggaccc acaggcggac acagccaacc cgagccccag actgatcaga
      901 acgacagcga gcaacttccc aatcaacgaa tcccgcaaat cacgaaccag gttcatggac
      961 gaaacgcctc tcctcgttgt acagaccttg agcacattgg ctcctctctc ccccgtcagg
     1021 ctagcgccaa aaccggcgct tcgggagaaa tccgtctcac ccgcctcgat tcaaccggtg
     1081 atcgcgccca gcgcggcttc ggtcaactgc cttgcgaccc ggtcctttga ccattcctga
     1141 tcgagtgcca gctgctcgac caccggcacc gaacagatcg cccaggacag gctcgcggcc
     1201 gaagtcgccg aaagcccgtc ccgcagcatt cccgccttcc ccagtcgcgc cgtcgccgca
     1261 cggaacccgg cgcgaagttc ccgggaacgg tcctcccagg cctttgccgc ggccgggtcg
     1321 gcctccctcg cggcgaagag catcgaggcg accggctgga tctccgccgc gtacctcagc
     1381 caccccttca cgaactggcg gaatgcctcg accgggtcct cccgcagtag cgcccgttcc
     1441 agctttt
//
LOCUS       DAOQMF010000593         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_72593_length_1445_cov_4.033094, whole genome shotgun sequence.
ACCESSION   DAOQMF010000593 DAOQMF010000000
VERSION     DAOQMF010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72593_length_1445_cov_4.033094"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLV77_09350"
     CDS             complement(<1..1171)
                     /locus_tag="PLV77_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16095.1"
                     /translation="MNGFQKLAVVILGVLALGLVGASGANAGTYVMRQCDGSSFLDFQ
                     GTYSAINASPHFDAVSGCVTSGSGKLGIYQDRSGTSLAYGEGGHFVWAAPTGTEVIGA
                     RIAARLNDKNNIQAQLNGYNVGVHTDIDGGIPHDGSDQVSYWSDPAHPQWMVVIELLC
                     QSVNGCANQVGGTKAFLEVTDTEFTVRDTVAPTAAASGTLWDWGGDSGYHRGTASIGI
                     DATDQGSGVAATWVEINGLRVDLPAPTCPGDKGDYTTRFTPCPLTYSTIRTFDTSVSP
                     FQEGSNQIRVCSRDYASSEANASKTCSATRTVLIDNRFSNPADNLRSDQGTDWQPVNG
                     FTLRWEIPEGQVAPIIGAVYKIQELETDTLVDAGYFSGNDIQSAGPINLPAVGAYK"
BASE COUNT          254 a          439 c          455 g          297 t
ORIGIN      
        1 ccttgtacgc gccgaccgcg ggcaggttga tcggaccagc gctctgaatg tcgtttccgc
       61 tgaagtagcc ggcgtcgacc agcgtgtcgg tctcgagttc ctgaattttg tagacggccc
      121 cgatgatcgg tgcgacctgg ccttccggta tctcccagcg aagggtgaac ccgttgacgg
      181 gttgccagtc ggttccctga tcggaacgca gattgtccgc cggatttgag aaccggttgt
      241 caatcaggac agtcctggtg gcgctgcatg tctttgaagc gtttgcctcg gagctcgcgt
      301 agtcacggga acagacccga atctggtttg acccttcctg aaagggcgag accgaggtat
      361 cgaaagtcct gatcgtcgag taagtcagcg ggcagggagt gaacctggtc gtgtagtcac
      421 ccttgtcacc cgggcaggtc ggcgccggga ggtcgaccct cagaccgttg atttcgaccc
      481 aggtggcagc cactcccgaa ccctggtcgg tggcgtcgat cccgatcgag gcagtacccc
      541 ggtgatagcc ggaatcgcca ccccagtccc agagggttcc cgaggccgcc gctgtcggcg
      601 cgaccgtgtc cctgaccgtg aactcggtgt cggttacctc gaggaaggcc ttggttccac
      661 ccacctggtt cgcgcacccg ttcaccgatt ggcagagcag ttcgatcacc accatccact
      721 ggggatgggc tgggtcagac cagtagctga cctgatctga accgtcgtga ggaatgccac
      781 catcgatgtc ggtatggact ccaacgttgt agccattcag ctgggcctgg atgttgttct
      841 tgtcattgag ccgggccgca atgcgggctc cgatcacttc ggttccggta ggagccgccc
      901 agacgaaatg accgccttca ccgtaggcga gactggtacc gctgcggtcc tggtagatgc
      961 ccagctttcc cgaacccgac gtgacgcatc cgctcacagc atcgaaatgc gggctcgcat
     1021 tgatcgcgga ataggtgcct tgaaaatcga gaaaactgga accgtcacat tgcctcatca
     1081 catacgtccc ggcattggct ccgctcgccc cgaccaggcc cagggccagt accccgagga
     1141 ttacgacagc cagtttctgg aatccgttca tggtgaaaac tcccctcctc cgtttccgac
     1201 aggcactgaa cttgaacttg aagctggttg agggggaggt gctgcagcgg gagccggcgt
     1261 tccggccgcc tcgggtgtga attccgtttc ggtcggagtc ggtgccggtg ccgactcggt
     1321 gatcaccggc gcttcactga ccggctcggg ggtggtcgac accggttcgg cggcggtagc
     1381 cgactgatcc ggagatccac cggcctgtga ttgcggggcc gtcgatggtt ccggcgcggg
     1441 ctcca
//
LOCUS       DAOQMF010000594         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_73087_length_1440_cov_3.514079, whole genome shotgun sequence.
ACCESSION   DAOQMF010000594 DAOQMF010000000
VERSION     DAOQMF010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73087_length_1440_cov_3.514079"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..>1440
                     /locus_tag="PLV77_09355"
     CDS             273..>1440
                     /locus_tag="PLV77_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNC16096.1"
                     /translation="MDLLMDLLLVPLDDAIVFPSVTATLQIDVGDEEQVFLLPRREGE
                     FARVGVIADVVEKGQSPRGDTVATVVGLRRGLAGVAHSDENDPDALRIEVQEIHDGHP
                     EDEHTRELVREYRAIVEEILELRNADSRIVSFLRNVTEPGELADTSGLSPDLSFDQKF
                     ELLDTLNVTARLEKAVEFQRERLAELQVRSKIRDHVESGAQQQQREYFLREQMKSIRK
                     ELGEDEGDVIEEYEQKIAEAGMPEAVEEQAVKELRRLERQGEQSGEGSMIRSYLDWLI
                     AVPWSKRSDEKLDPAYTREVLDADHAGLDDVKRRITEFVAVRKLRKEREAEADSRADG
                     AILTLVGPPGTGKTSIGESVAKALGREFVRISLGGVRDEAEIRGHRRTYIGSMPG"
BASE COUNT          268 a          475 c          473 g          224 t
ORIGIN      
        1 tcaccggcca ggtctatgcc ttctacacct ggctgctctg gccggtcctg ctgcgctcca
       61 gcgtccggca ggtgatcggc cgcggatcct gggccaagac ctcccgggag cagatcaccc
      121 ccgctgacga acccccggcc tcgaacccgg cctgacccct cacccgaggc ttcctgtatc
      181 ggggtgggaa tcgggttcgt cccgatgtcc gaggcagttg gttgacagcg caacaacttg
      241 cgctacactg tgcgaaggac taactacaga aattggactt actaatggat cttctgctgg
      301 ttccacttga tgacgcaata gttttcccct cggtcacggc gacgctccag atcgacgtcg
      361 gtgacgagga acaggtcttt ctgctgccgc gccgcgaagg cgagttcgcc cgggtagggg
      421 tgatcgccga cgtggtcgag aaggggcagt ccccccgtgg cgacaccgtt gccacggtgg
      481 ttggcctgcg tcgcggcctg gccggtgtgg cccattccga cgagaatgat ccggacgcgc
      541 tccggatcga ggttcaggag atccacgacg gccaccccga ggacgagcac accagggaac
      601 tggttcgcga gtaccgggcg atcgtcgagg agatcctcga actcagaaac gccgacagcc
      661 gcatcgtcag ctttctgcgc aacgtgaccg agccgggcga gctcgccgac acctccggcc
      721 tcagccccga cctcagcttc gaccagaagt tcgagctgct cgacacgctc aacgtgaccg
      781 cccgcctcga gaaggcggtc gagttccagc gcgagcgcct ggccgagctg caggtccgca
      841 gcaagatccg cgaccacgtc gagtccggcg cccagcagca gcagcgcgag tacttcctgc
      901 gcgagcagat gaagtcgatc cgcaaggagc tcggcgagga cgaaggcgac gtaatcgagg
      961 agtacgagca gaagatcgcc gaagcgggga tgcctgaagc cgttgaggaa caggccgtga
     1021 aggaactgcg ccggctcgag cgtcagggcg agcaatcggg cgagggctcg atgatccgtt
     1081 cctacctcga ctggctgatc gcggtcccgt ggagcaaacg ctccgacgag aaactcgatc
     1141 cggcctacac ccgcgaggta ctcgatgccg accacgccgg tcttgatgac gtgaagcggc
     1201 ggatcaccga attcgtcgcg gtgcggaagc tccgcaagga gcgcgaggcc gaggccgaca
     1261 gccgggctga cggcgcgatc ctgaccctgg tcggaccgcc cggtaccggc aagacctcga
     1321 tcggcgagtc ggtcgccaag gccctcgggc gcgagttcgt ccggatttcg ctcggcggcg
     1381 tgcgtgacga ggccgagatc cgcggccacc gccgcaccta catcggctcg atgcccggca
//
LOCUS       DAOQMF010000595         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_73210_length_1439_cov_3.168353, whole genome shotgun sequence.
ACCESSION   DAOQMF010000595 DAOQMF010000000
VERSION     DAOQMF010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73210_length_1439_cov_3.168353"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLV77_09360"
     CDS             <1..452
                     /locus_tag="PLV77_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC16097.1"
                     /translation="TLDRLSEVLPPAPTGIDLNPESVEFAARRLPGATIRQADLLNLP
                     FEDGSFDLVFCLEVLEHLPDPAAGLAELARVSGRDIVVSVPHEPWFRLGSFARGKYLK
                     TWGNHPEHVNHWNPKSLKAFLSTRTEVVEVQTSMPWIIARCRPLPAG"
     gene            complement(418..>1439)
                     /locus_tag="PLV77_09365"
     CDS             complement(418..>1439)
                     /locus_tag="PLV77_09365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC16098.1"
                     /translation="DAAYYLDGQAMDLSLIIPAYNESEALPGLIAEIDSACEGLGLQW
                     ETIVIDDGSTDGTFGVISEIAKSNPRVGVVRLRRNFGKSVALAVGFDVTRGEKVITID
                     GDGQDDPADIPRLLEGLENGSDLVSGWKSDRQDPVTRRLASRIFNWFTARVTGVDLHD
                     MNCGFKAYRGECARSIEVYGEMHRFLPAFAAQQGWRVTELPVNHRPRLHGSSRFGLER
                     YRRGALDLLTVAFLGRYQNRPLHLFGLLGLVLGSLGFLICLYLSIIWIGGEAIGERPL
                     LFLGVLLIVVGVQLFSLGLLGQMMVVGRRAGVSEEKVLIEKVLSPESDPELGLSRLEA
                     VGSGR"
BASE COUNT          260 a          492 c          459 g          228 t
ORIGIN      
        1 aaaccctgga tcgcctgagc gaggttctgc cgcccgcccc caccgggatc gacctcaacc
       61 ccgagtcggt cgagttcgcc gcccggcgac tccccggcgc gaccatccgg caggccgacc
      121 tgctcaacct gccgttcgag gacggcagct tcgacctcgt cttctgcctc gaggtgctcg
      181 aacacctgcc cgacccggct gccggcctgg ccgaactggc ccgggtttcc ggccgggaca
      241 tcgtcgtttc ggtgccgcat gagccctggt tccggctcgg cagcttcgcc cgcggcaagt
      301 acctcaagac ctggggcaac catcccgagc atgtgaacca ctggaatccg aagagcttga
      361 aggccttcct ttcgacccgg accgaagtgg tcgaggttca gacctcgatg ccctggatca
      421 tcgcccgctg ccgaccgctt ccagccggct gagaccgagt tccggatcgc tttcggggct
      481 gagcaccttc tcgatcaaca ccttctcctc ggagacaccg gcccgccggc cgaccaccat
      541 catctggccg agcaggccca gtgagaacag ctggaccccg accacgatca gcagcacccc
      601 gaggaagagc agcggtcgct cgccgatcgc ctcgccaccg atccagatga tcgagaggta
      661 gaggcagatc agaaaaccaa gcgagccgag gacgagcccg agcaggccga aaaggtgcag
      721 gggacggttc tggtagcggc cgagaaaggc gacggtcagc aggtcgaggg cgccgcgccg
      781 gtagcgctcg aggccgaagc gggaagagcc gtggaggcgg ggacggtgat tcaccggcag
      841 ctcggttacc cgccagccct gctgggcggc gaaggccggc aggaagcggt gcatctcccc
      901 gtagacctcg atcgagcggg cgcattcgcc gcggtaggcc ttgaagccgc agttcatgtc
      961 atgcaggtcg actccggtaa cccgggcggt gaaccagttg aagatcctcg atgccaggcg
     1021 acgggtgacg gggtcctggc ggtcgctctt ccagcccgag accaggtcgg acccgttttc
     1081 caggccttcg agcagtcgcg ggatgtcggc cgggtcatcc tggccatcac cgtcgatcgt
     1141 gatcaccttc tcgccgcggg taacatcgaa gccgaccgcc agggcgaccg acttgccgaa
     1201 gttccgcctc agcctgacga ccccgacccg gggattggac ttcgcgatct ccgagatcac
     1261 cccgaaggtg ccatcggtcg agccatcatc gataacgatc gtctcccact gaaggccgag
     1321 cccttcgcag gcggaatcga tctcggcgat cagcccgggc agggcttcgc tctcgttgta
     1381 ggcgggaatg atcagggaca ggtccattgc ttgcccgtca aggtaatacg ctgcgtcgc
//
LOCUS       DAOQMF010000596         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_73233_length_1439_cov_2.560694, whole genome shotgun sequence.
ACCESSION   DAOQMF010000596 DAOQMF010000000
VERSION     DAOQMF010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73233_length_1439_cov_2.560694"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLV77_09370"
     CDS             <1..816
                     /locus_tag="PLV77_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16099.1"
                     /translation="QGSLPTVSITGPGDGLTFDFNSAHFLELTCGTGAGPDLESCLAR
                     DQAPDGETNLVTGDRLPTDQPGTHTLTATATNELGLTATDTVTYEVGPGCFADGVRLA
                     SVQPRGAKVKLLGAADPSSAGRQARILRAGKRAGTATVTGDGRIEATVSAPKSKRLRP
                     GTRYRLVVGGSRSNPMKPSGAVRVLSRKPLPDADLIKGKLAGVRKQGRFTLRARPLCG
                     GPATSRQVSFNRSGVFSVRIGFTSATRTFSIRRSGGEVPLPVVMPAARFITGN"
     gene            complement(824..>1439)
                     /locus_tag="PLV77_09375"
     CDS             complement(824..>1439)
                     /locus_tag="PLV77_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNC16100.1"
                     /translation="PTRVAQLRRGLDLVSHPGWVRRVPIAGKPLTFGNLAEVVPGART
                     PEDFKEWVDAQFDPSVTWDDIAWVRENWKGKLVVKGVLDPEDARKAVDAGVDGVVVSN
                     HGGRQLDAVPSTAAALPGVAEAVGDETTVLADGGIRNGLDMVKMLALGAEAVIIGRAW
                     AWAVAAEGQTGVEKMLRVLKSDADVALALTGQNSVAGLGPDSLV"
BASE COUNT          227 a          525 c          480 g          207 t
ORIGIN      
        1 cagggcagcc ttccgacggt gtcgatcacg ggccccgggg acgggctcac gttcgacttc
       61 aactcggctc atttcctcga attgacctgc ggtaccgggg ccgggccgga tctcgagtcc
      121 tgcctcgcca gggaccaggc cccggacggg gagacgaatc tggtcaccgg cgaccggctg
      181 ccgaccgatc agccgggcac ccacaccctg accgcgaccg cgaccaacga gctggggctg
      241 accgcgactg acacggtcac ctacgaggtc gggccgggct gcttcgctga cggggtcagg
      301 ctggcctcgg tccagccccg gggagcaaag gtgaagctgc tcggtgccgc cgacccctcc
      361 agcgccggcc gccaggccag gatcctgcgg gccggcaagc gggccggcac cgcgaccgtc
      421 accggagacg gcaggatcga agcgacggtc agcgccccga aatcgaagcg gcttcgcccc
      481 gggacgcgct accggctggt cgtcggtgga agcaggtcga atccgatgaa gccaagcgga
      541 gcggtcaggg tcctttcccg caagccgctt ccggatgccg acctgatcaa gggaaaactg
      601 gccggggtca ggaagcaggg ccggtttaca ctgcgggccc gcccgctctg cggcggtccg
      661 gccacttcgc gccaggtcag cttcaaccgc agcggcgtct tttcggtccg gatcggtttc
      721 acgagcgcca cccggacctt ctcgatcagg cggtccggcg gggaagtccc cctcccggtg
      781 gtcatgccgg ccgcccggtt catcaccggg aactgaaccc cgctcagacc agcgagtccg
      841 gacccaggcc ggcgaccgag ttctgaccgg tcagcgcgag ggcgacgtcg gcgtcggact
      901 tcagcacccg gagcatcttc tccaccccgg tctggccttc cgcggcaaca gcccaggccc
      961 aggcccggcc gatgatcacg gcttcggccc cgagggcgag catcttgacc atgtcgaggc
     1021 cgtttcttat gccgccgtcg gcaagcaccg tggtctcgtc cccgaccgct tcggcaaccc
     1081 cgggcagggc ggctgcggtc gatggcaccg cgtcaagctg ccggccaccg tgattggaga
     1141 cgaccacccc gtcaaccccg gcgtcgaccg ccttgcgggc atcctccggg tcgagcacac
     1201 ccttgaccac cagcttgccc ttccagttct cccgcaccca ggcgatgtca tcccaggtga
     1261 ccgaaggatc gaactgggcg tcgacccatt ccttgaagtc ctccggggtg cgggcgccag
     1321 gcacaacttc ggccaggttg ccgaaggtca gcggcttgcc ggcgatcggc acgcgcctga
     1381 cccagcccgg atgggaaacg aggtcgagtc cgcgcctcag ctgggcgacc cgggtcggc
//
LOCUS       DAOQMF010000597         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_73467_length_1437_cov_1.976122, whole genome shotgun sequence.
ACCESSION   DAOQMF010000597 DAOQMF010000000
VERSION     DAOQMF010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73467_length_1437_cov_1.976122"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1437)
                     /locus_tag="PLV77_09380"
     CDS             complement(<1..>1437)
                     /locus_tag="PLV77_09380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC16101.1"
                     /translation="NRVEALLGTGRFDIVHVHDPFVPGFSSTALRHSFSLNVGTFHET
                     EERPFATQFARPLLEIFFGRLDARTASSKTSAKLLNRYFPGSYEITHPGVDPAEPARV
                     EVPPLKIAFSEREEPGALRLFLRALRRLPADLDWTASIYADDPGEVQLRVASAIRDRI
                     KVIGPEQVSLADLLSHSDVFVAASNGPRPDPAAVRQAIASGAVPVTTTIGRYVELVGD
                     GQRGLLFPAGDPVTLAGQIQRLGEDSRLREGFRSQCGKFEVESWSGVAGRFEAIYERL
                     VSRRHDPAGNEELRKKLRDRPLIEVDLHMHTDHSSDCATPVKVLIETARDRGFGAIAI
                     TDHNEVSGAMEAAEVAAEMDDFKIIVAEEVMTGDAGEVIGLFLKEKIPKGTSMSDTIR
                     EIKRQGGLVYVPHPFDRLHSVPDYENLLDIVEDIDLMEVFNPRVAISVFNEEAERFAR
                     KYNIIPAAGSDSHVAQGLGAVRNRLHDFD"
BASE COUNT          240 a          446 c          513 g          238 t
ORIGIN      
        1 gtcgaaatcg tggaggcggt tgcgaaccgc cccgagaccc tgggcgacat ggctgtcgga
       61 gccggccgcg gggatgatgt tgtacttgcg ggcgaaccgt tcggcttcct cgttgaagac
      121 ggagatcgcg acgcgggggt tgaagacctc catcaggtcg atgtcctcga cgatgtcgag
      181 caggttctcg taatcgggga ccgagtggag gcggtcgaag ggatgcggca catagacgag
      241 gccgccctgg cgcttgatct ccctgatcgt gtccgacatc gaggttccct tcgggatctt
      301 ctccttgagg aagaggccga tcacctcgcc ggcgtcaccg gtcatcactt cctccgcgac
      361 gatgatcttg aagtcatcca tctccgccgc gacctccgcg gcttccatgg ccccggagac
      421 ctcgttgtgg tcggtgatcg cgatcgctcc gaagccgcgg tcgcgggcgg tttcgatcag
      481 caccttgacc ggggtcgcgc agtcactcga atggtcggtg tgcatgtgga ggtcgacctc
      541 gatcagcggc cggtcgcgca gcttcttcct cagctcttcg ttgccggccg gatcatggcg
      601 gcgggagacc agccgctcgt agatcgcctc gaatcgcccg gccaccccgg accagctttc
      661 aacctcgaac ttgccgcact gcgaccggaa accttcacgc agccgggagt cctcgcccag
      721 ccgctggatc tggccggcca gcgtgaccgg gtcaccggcc gggaagagca gaccgcgctg
      781 gccgtccccg accagttcga catagcggcc gatcgtggtc gtgaccggca ccgcgccgga
      841 agcgatcgcc tggcgaacgg ccgccgggtc cggccgcgga ccgttggagg cagccacgaa
      901 gacatcggaa tgggagagca ggtctgcgag cgaaacctgt tcgggaccga tcaccttgat
      961 ccggtcgcga atggcgctgg ccacacgaag ctggacttcg ccgggatcgt ccgcgtagat
     1021 gctggcggtc cagtcgaggt cggccggcag gcgccgcagg gcccgcagga agaggcggag
     1081 cgcccccggt tcctcacgct cggagaaggc gatcttgagc ggtggcacct cgacccgggc
     1141 cggctcggcc gggtcgaccc cggggtgggt gatctcgtat gaaccgggga agtaacggtt
     1201 gagcagcttg gccgaggttt tcgaggatgc ggtcctcgcg tcgaggcggc cgaagaagat
     1261 ctcgagcagg ggccgggcga actgggtcgc gaagggccgc tcctcggtct cgtgaaaggt
     1321 gccgacgttg agcgagaagg aatggcgcag ggcggtcgag gagaagccgg gcacgaaggg
     1381 atcgtggacg tggacgatgt caaagcgacc ggtcccgagc agcgcttcga cccggtt
//
LOCUS       DAOQMF010000598         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_73747_length_1434_cov_2.941262, whole genome shotgun sequence.
ACCESSION   DAOQMF010000598 DAOQMF010000000
VERSION     DAOQMF010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73747_length_1434_cov_2.941262"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLV77_09385"
     CDS             <1..323
                     /locus_tag="PLV77_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16102.1"
                     /translation="KGQSAAQHEETGKTAVETATQLAAFYKAGEKAGGNGTGPGGDTS
                     EQAAVMAGYLVGAIEKGAEDTEGIHAALVDRVEAAATNGLEGANQQIQGAYQKGYAAG
                     QAKG"
     gene            393..>1434
                     /locus_tag="PLV77_09390"
     CDS             393..>1434
                     /locus_tag="PLV77_09390"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNC16103.1"
                     /translation="MSEQLWGGETTKAIENFPVSGEKIPPSLVKWLGLIKANAARANA
                     ELGLLDTDIADRIAKAGDEVGAGRHDDQFPIDIFQTGSGTSSNMNANEVIANLAGEGV
                     HQNDHVNMGQSSNDVFPSAVHLAALDQVTHDLLPAMEQLAVALEAKAEEFRDVVKAGR
                     THLMDAVPVTLGQEFGGYASQVRHGIDRVRGTLPRVGQIPLGGTATGTGLNTHPEFSA
                     KVRGLLEAESGLAIAPPHDRFEAQANRDSLVELSGALKVYAVSLNKIANDLALMGSGP
                     RTGLAELHLPELQKGSSIMPGKVNPVIPEVVIQVAAQVVGNDMAITMAGSQGQFELNV
                     RVPLIARNLLGSI"
BASE COUNT          279 a          491 c          468 g          196 t
ORIGIN      
        1 ccaaggggca atccgcggcc cagcatgaag aaaccggcaa gaccgcggta gagacggcca
       61 cccagctggc cgccttctac aaggccggcg aaaaggcggg cggcaacggg accgggcccg
      121 gtggcgacac ctccgaacag gcggcggtga tggccggtta cctggtcggg gcgatcgaga
      181 agggggccga agacaccgaa ggtattcacg ccgccctggt cgaccgggtc gaagccgccg
      241 ccaccaacgg gctcgaaggg gccaaccagc agattcaggg cgcctaccag aagggttacg
      301 cggccggcca ggcgaagggc taggcgaacg ccgttccggt gccgggctga aagactgtcc
      361 gcagcaagtc agtcaaagcc ggaaggacag aagtgagcga gcaactctgg ggtggcgaaa
      421 ccaccaaggc gatcgaaaat ttccccgtgt ccggcgagaa gattccgccc tcgctggtca
      481 agtggctggg gctgatcaag gccaacgccg cccgggccaa cgcggaactg ggactgctcg
      541 acaccgacat cgccgaccgg atcgcgaagg ccggggacga ggtcggagcg ggcaggcatg
      601 acgaccagtt cccgatcgac atcttccaga ccggttccgg cacctcctcg aacatgaatg
      661 cgaacgaggt gatcgccaac ctggccggcg aaggagtcca ccagaacgac cacgtgaaca
      721 tgggccagtc gtcaaatgac gtcttcccct cggccgttca cctcgctgcc ctcgatcagg
      781 tcacccacga cctgctgccg gcgatggaac agctggcggt tgcgcttgaa gccaaagccg
      841 aggagttcag ggacgtggtc aaggccgggc gcacccacct catggacgcg gttccggtca
      901 ccctcggcca ggagtttggc ggctacgcct ctcaggtccg tcacggcatc gaccgggtcc
      961 ggggcaccct gccccgggtc ggccagatcc cgctcggcgg caccgcgacc ggcaccggtc
     1021 tgaacaccca ccccgaattt tccgccaagg tccgcggcct gctcgaggcc gagagcggtc
     1081 tcgcgatcgc cccgccgcat gaccgcttcg aagcccaggc caaccgcgac tccctggtcg
     1141 agctttccgg cgcgctcaag gtctacgcgg tttcgctcaa caagatcgcc aacgacctcg
     1201 ccctgatggg ctccggcccg cgcaccggcc tggccgagct tcacctgccc gagctgcaga
     1261 agggatcttc gatcatgccc ggcaaggtca acccggtaat tcccgaggtg gtgatccagg
     1321 tcgccgccca ggtggtcggc aatgacatgg cgatcaccat ggccggctcc cagggccagt
     1381 tcgagctgaa cgtgcgggtg ccgctgatcg cccgcaacct gctcggctcg atcc
//
LOCUS       DAOQMF010000599         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_73812_length_1433_cov_4.427431, whole genome shotgun sequence.
ACCESSION   DAOQMF010000599 DAOQMF010000000
VERSION     DAOQMF010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73812_length_1433_cov_4.427431"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1123
                     /locus_tag="PLV77_09395"
     CDS             182..1123
                     /locus_tag="PLV77_09395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNC16104.1"
                     /translation="MGDLILYAIPFFFLAMGLEYLTLRHAAHEHGEAQAANPDAPIGF
                     EAKDTATSLSMGVGHLFIAGAWKLVVLVIYAAIYEVSPIQLSPSDWWTWVLLFFADDL
                     AYYCFHRSHHRIRLFWAMHVVHHSSEHYNFSTALRQDWSPFSSIFFWMPSALLFPPWM
                     IYLAFSWSLLYQFLLHTEAIDRLPRPIEFIFNTPSHHRVHHGSQEQYLDKNYGGILII
                     WDRMFGSFEPEEERVRYGLTKNIKTFHPVKVAYGEYMNIWRDVRAADSWRDRFGYAFK
                     GPGWKPEPIAADDERIATDSHRRSDGTDRDRRLAAAD"
     gene            1153..>1433
                     /locus_tag="PLV77_09400"
     CDS             1153..>1433
                     /locus_tag="PLV77_09400"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HNC16105.1"
                     /translation="MAADSSAKSLPAPGWYPVPGRDRDTTVERHWDGEVWTDEVRTAP
                     VGSSLATYRRHFWGFVRNGNWKLLLVLILTLAGAAALWGTDRKADLVGG"
BASE COUNT          242 a          492 c          428 g          271 t
ORIGIN      
        1 cgacccccgg atcatcaccc ggctcggcaa cgcgatcggc gccggcgacc ccgagacggc
       61 ggccgaggtc gctggccaca tgctgcgcag cgacatcgac cgcgtcgcct gaaccggatc
      121 ccggctgcgg ccgatccgcc ccttgcaacc gtctgggtga actggtataa ccagttcggc
      181 aatgggcgat ctgattcttt acgcgatccc gttcttcttc cttgcgatgg gtctggagta
      241 cctgaccctg cgccacgcgg cgcatgagca cggagaagcg caggccgcaa acccggacgc
      301 gccgatcggc ttcgaagcga aggacacggc caccagtctc agcatggggg tcggccacct
      361 gttcatcgcc ggcgcctgga agctggtcgt tctcgtcatc tacgccgcga tctacgaagt
      421 cagcccgatc cagctttcac cttcggactg gtggacctgg gtgctgctct tcttcgccga
      481 cgatctcgcc tactactgct tccaccgcag ccaccaccgc atccggctct tctgggcgat
      541 gcatgtggtt caccactcct ccgagcatta caacttctcg accgcgcttc gtcaggactg
      601 gtcgccgttc agctcgatct tcttctggat gccttcggcc ctgcttttcc cgccgtggat
      661 gatctacctc gcgttttcat ggagcctgct ctaccagttc ctgctccaca ccgaggcgat
      721 cgaccggctg ccccggccga tcgagttcat cttcaacacg cccagccacc accgggtcca
      781 ccacggctcc caggagcagt acctcgacaa gaactacggc ggaatcctga tcatctggga
      841 ccgcatgttc ggcagcttcg aaccggagga agaacgggtt cgctacggcc tgaccaaaaa
      901 catcaagacg ttccatccgg tcaaggtcgc ctacggcgaa tacatgaaca tctggcgtga
      961 cgtgcgggcc gccgacagct ggcgggaccg gttcggctac gccttcaagg gtccgggctg
     1021 gaaacctgag ccgatcgccg cagacgatga gcggatcgcg accgattcac atcggcgctc
     1081 cgacggcacc gatcgggatc gacggctggc tgctgctgat tgatccgctg gttccggggt
     1141 agctaccgtg ggatggctgc cgactcttcc gcgaagagcc ttcccgcacc tggctggtac
     1201 cccgtcccgg gccgcgatcg ggacacgacc gtcgagcgcc actgggacgg cgaagtctgg
     1261 accgacgagg tcaggaccgc tccggtcggt tcaagtctcg ctacctaccg gaggcacttc
     1321 tggggcttcg tccggaacgg caactggaag ctcctgctgg tcctgatcct gacccttgcc
     1381 ggcgcggcgg cgttgtgggg taccgaccgc aaggccgatt tggtcggcgg cat
//
LOCUS       DAOQMF010000600         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_73830_length_1433_cov_3.657475, whole genome shotgun sequence.
ACCESSION   DAOQMF010000600 DAOQMF010000000
VERSION     DAOQMF010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73830_length_1433_cov_3.657475"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /gene="tatC"
                     /locus_tag="PLV77_09405"
     CDS             <1..778
                     /gene="tatC"
                     /locus_tag="PLV77_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNC16106.1"
                     /translation="AAVARLKPVKHDEHLSLVDHLDELRTRIIYSLVFLGVAFALCFW
                     QSDLILDIAAAPLPEDHKALVVLAPTEAFMTTVTVCAYAAIIIAAPFISYQVFAYILP
                     AFSPRERKAILPMLITIPMLFLVGCVFAYFVVLPAAINFLLSFNSEQFAIQLRAREYY
                     SFFSMTLLAGGVVFELPIVILALVRLRILTVDQLRQNRRYAYLICAVIAAALPGVDPI
                     SMLIETVPLLVLYELSILLARGIGQPKESAESQIPSIQEN"
     gene            783..>1433
                     /locus_tag="PLV77_09410"
     CDS             783..>1433
                     /locus_tag="PLV77_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16107.1"
                     /translation="MANEPEHKMLFDVRGRRKRVIQVIYVILAVIMAASLIVIGLPGG
                     INPFNNANSGVNSDYAKANVERADKLQKQLQAQPANEKLAVELIRARLSAGQSLSETD
                     ASGQIVTTDEAADQYDMAASTWTRYLKMTDNNPDPEVAQLMATILFQLSQGSTVAQFQ
                     ANIEDAATAQQFVADNAVKEQKKGGVSAWQQLTTLFLYQLYAQDYAAAEKTKQQALD"
BASE COUNT          276 a          489 c          404 g          264 t
ORIGIN      
        1 ggccgccgtg gccaggctca aaccggtcaa gcatgacgag cacctcagcc tcgtcgacca
       61 tctggacgag ctgcgcaccc ggatcatcta ttcactggtc tttctgggcg tcgccttcgc
      121 gctctgcttc tggcagagcg acctgatcct cgacatcgcg gcggccccgc tcccggaaga
      181 tcacaaggcc ctggtcgtgc tcgctccgac cgaagccttc atgaccacag tgacggtctg
      241 cgcctacgcg gcaatcatca tcgcggcccc cttcatcagc taccaggtat ttgcctacat
      301 cctgccggcc ttctcgccgc gggaacgcaa ggcgatcctg ccgatgctga tcacgatccc
      361 gatgctcttc ctggtcggct gcgtcttcgc ctacttcgtg gtgctgcccg ccgcgatcaa
      421 cttcctgctc agcttcaaca gcgaacagtt cgcgatccag ctgagagccc gggagtacta
      481 ctcgttcttc tccatgaccc tgctcgccgg tggggtcgtc ttcgagttgc cgatcgtgat
      541 tctcgccctg gtcaggctga ggatcctgac cgtcgaccag ctccggcaga accgtcgtta
      601 cgcctatctg atctgcgcgg tcatcgccgc cgccctgcct ggagtcgacc cgatttcgat
      661 gctgatcgag acggtcccgc tactggttct ctacgagttg agtatcttgc tcgctcgggg
      721 gatcggtcag cccaaggaaa gcgccgagtc ccagattcct tcaattcagg agaactagcc
      781 aagtggccaa cgaacccgaa cacaagatgc ttttcgacgt gcgcggtcgg cgcaagcggg
      841 tgattcaggt catttacgtg atcctcgccg tgatcatggc ggccagcctg atcgtgatcg
      901 gactgcccgg cggcatcaac ccgttcaaca acgccaacag cggggtcaac agtgattacg
      961 ccaaggcgaa cgtcgagcgg gccgacaaac tccagaagca gctccaggcc cagcccgcca
     1021 acgagaagct cgcggttgaa ttgatccggg cccggctctc ggccggccag tcgctgtcgg
     1081 agaccgacgc cagcggccag atcgtgacca ccgacgaagc cgccgaccag tacgacatgg
     1141 cggcttcaac ctggaccagg tacctcaaga tgacggacaa caatccggat cccgaggtcg
     1201 cccagctgat ggccacgatc ctcttccagc tttcccaggg ttcgaccgtc gcccagttcc
     1261 aggcgaacat cgaggacgcg gccacggcgc agcaattcgt ggcggacaac gcggtcaagg
     1321 aacagaagaa gggcggagtc agcgcctggc agcagctcac caccctcttc ctctaccagc
     1381 tatacgccca ggattacgcg gccgccgaga agacgaagca gcaggctctc gac
//
LOCUS       DAOQMF010000601         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_73847_length_1433_cov_3.094340, whole genome shotgun sequence.
ACCESSION   DAOQMF010000601 DAOQMF010000000
VERSION     DAOQMF010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73847_length_1433_cov_3.094340"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..>1433
                     /locus_tag="PLV77_09415"
     CDS             151..>1433
                     /locus_tag="PLV77_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16108.1"
                     /translation="MSFRRFFVAFLMLAAILVLPQSASALLPHSTTKRLDDGTERITY
                     RIGPLTVTPGQNRIAFKPLTGDEKPSVDGWITRIKPNMVNEDGSIPLSSKVMFHHGVW
                     LNFSRQDATSGGQERFFATGEEKTITRFPDGYGYQYKASDVWLLNHMIHNLTPEAMTL
                     YATYTIDFIPADSPAADGIKPVTPIWMDVDNGSVYPVFDIWKGSGGKDGKFTYPDDAD
                     NPYPNGVAKNTWTVDRDGVLLATAGHVHTGGLYTDLYLQRPGARYAGPKCVQPKAARP
                     PNRRNIRRYGAKLKKYKSCKASMPSVKGSKVHLFRSQAHYFEPAGPVSWDMAMVGTRD
                     DWRPEVKKGDVLSMNSTYETKLASWPESMGIMVVYMAEGEHGTNPYRKKVDYKGQLIH
                     GHYAENNDHGGKLPVVGPDPTRLPDGANPDGNPFT"
BASE COUNT          309 a          490 c          415 g          219 t
ORIGIN      
        1 tcaccgcagg acagtcgaag acagtcaacc tgccgttgac caaggccggc cgcaaggcaa
       61 tcgccaggcc gaagtctgtc cagatcaaac tgagcgccgc cgtgccctac ggcaagtcgg
      121 cgagcgcctc ccgaaaggtc aagtagccag atgagcttcc gcaggttctt cgtcgcattt
      181 ctgatgctcg ccgcgattct ggtcctgccc cagtcggctt cggcactgct tcctcacagc
      241 accaccaaac gtctcgatga cgggaccgaa cgaatcacct accggatcgg cccgcttacc
      301 gtcacccccg gccagaaccg gatcgccttc aagccgctga ccggcgacga gaagccctcg
      361 gtggatggct ggatcacccg gatcaagccg aacatggtca acgaggacgg ctcgatcccg
      421 ctttcgagca aggtcatgtt ccaccacggt gtgtggctca atttcagccg ccaggacgca
      481 acctcgggtg gtcaggaacg cttcttcgca accggtgagg aaaagacgat cacgcggttc
      541 cccgacggtt acggctacca gtacaaggca tccgacgtct ggctcctcaa tcacatgatc
      601 cacaacctga cgccggaagc gatgacgctc tacgcgacct acacgatcga cttcatcccg
      661 gctgattcac cggccgccga cgggatcaag ccggtcaccc cgatctggat ggacgtggac
      721 aacggcagcg tctacccggt cttcgacatc tggaagggca gcggcggcaa ggacggcaag
      781 ttcacctacc cggatgacgc cgacaatccc tacccgaacg gcgtagccaa aaacacctgg
      841 accgttgatc gcgacggcgt cctgctcgcc accgccggcc acgtccacac cggtggcctc
      901 tacaccgacc tctacctgca gcgccccggc gccaggtacg ccggccctaa gtgcgtgcag
      961 cccaaagcgg cccgaccgcc gaaccgcagg aacatccgtc gttacggggc gaagctgaag
     1021 aagtacaaat cctgcaaggc ttcgatgccg agcgtcaagg gcagcaaggt ccacctcttc
     1081 cgttcgcagg cgcattactt cgagccggcc ggtcccgttt cctgggacat ggcgatggtc
     1141 ggcaccaggg acgactggcg gcccgaggtc aagaagggcg acgtcctctc gatgaactcc
     1201 acctacgaga cgaagctggc ctcctggccc gagtcgatgg ggatcatggt cgtctacatg
     1261 gccgagggcg agcacggcac caacccgtac cggaagaagg tcgactacaa gggccagctg
     1321 atccacggtc attacgcgga gaacaacgat cacggcggca agctgccggt ggtcggaccc
     1381 gacccgacca ggctgccgga cggagcaaac cccgacggga acccgttcac gat
//
LOCUS       DAOQMF010000602         1427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_74494_length_1427_cov_2.345481, whole genome shotgun sequence.
ACCESSION   DAOQMF010000602 DAOQMF010000000
VERSION     DAOQMF010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74494_length_1427_cov_2.345481"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PLV77_09420"
     CDS             complement(<1..1382)
                     /locus_tag="PLV77_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16109.1"
                     /translation="MPWSDMAYALAPNGGTLDYASSSPYRKPYGVMKSGIVNGLFATG
                     ENFSGSYGAPVDPLFDVIGWKDELAAGEPYGNDPLIATAVGELTRFHSAGYIDDSVTP
                     APMFIAQGLTDDIFPVDEAIRYYNRIKASHPDARIGLYLADIGHPRALLATQGRPADI
                     RIADARVEEWFAHYLLGDGPEPETTVVARSQVCPYEEPSGGPFTADTWAKLAPGEVRI
                     SDPGRHVIEATSGDDGVAAGFISILPGCSQMPDTAEPGTWSRDFPAPGGDGYTVAGST
                     TVIADISSPNGGESEIAARLLEVTGGQERLIGRALYRPARSGRQVFQLHANVYAIGPD
                     AHLRLQLLPRDGMTGPSAAASYGRPSSDQRDITISDIEVRVPVAEHPGEAGGQVRQPL
                     RKVLSAGRSLAAQFRPTGAARPTGAAQVDRLRIAGRRLSLRVRCRADTDRCLSGRLVI
                     RGYRRGGPVDR"
BASE COUNT          216 a          475 c          513 g          223 t
ORIGIN      
        1 cggtcgaccg ggccgccacg gcggtaaccg cggatcacca gccggccgga caggcagcga
       61 tccgtgtcgg cacggcagcg caccctcaag ctcagtcgac ggccggcgat tcgcagccga
      121 tcgacctgcg ccgcaccggt tggcctggcc gctccggtcg gccggaactg ggcggccagg
      181 ctgcggccgg ccgacaggac cttccgcagc ggctgcctga cctggcctcc tgcctccccg
      241 ggatgctcgg cgaccgggac ccggacctcg atgtcggaga tcgtgatgtc ccgctggtcg
      301 ctcgaaggcc gcccgtagga agccgccgcg ctggggccgg tcatcccgtc cctgggcagg
      361 agttgcagcc ggagatgcgc gtccgggcca atcgcgtaaa cgttcgcgtg gagctggaac
      421 acctgccggc cggagcgggc cggacggtag agcgcccttc cgatcaagcg ctcctggcct
      481 ccggtcactt ccaggagccg ggccgcgatc tctgactcac cgccgttcgg agaggagatg
      541 tcggcgatca ccgtggtcga gccggcgacc gtgtagccat ccccgccggg ggccgggaag
      601 tcccgggacc aggtacccgg ttccgcggtg tccggcatct gcgagcagcc gggcaggatg
      661 ctgatgaaac cggcggcgac accatcgtct cccgaagtgg cctcgatcac atggcggccc
      721 gggtccgaga tgcgcacctc gccgggggcc agcttcgccc aggtgtcggc ggtgaagggt
      781 ccgcccgagg gttcctcgta cgggcagacc tgggagcggg cgacaaccgt ggtctccggc
      841 tcgggtccgt caccgagcag gtagtgggcg aaccattcct cgaccctggc gtccgcgatc
      901 cggatgtctg cgggcctgcc ctgggtggcc agcagggccc ggggatgacc gatgtcggcg
      961 aggtagagcc cgatcctcgc gtcggggtgc gaggccttga tccggttgta gtagcggatt
     1021 gcctcgtcta ccgggaagat gtcgtcggtc agtccctggg cgatgaacat cggggccggg
     1081 gtcaccgaat cgtcgatgta gccggccgaa tggaatctgg tcaactcgcc gaccgcggtg
     1141 gcgatcagcg ggtcgttccc gtagggctcg cccgcagcca gctcgtcctt ccagccgatc
     1201 acgtcaaaga gcggatcgac cggggctccg taactgccgc tgaagttctc gccggtagcg
     1261 aacaagccgt tgacgattcc ggacttcatc accccgtagg gcttgcggta gggagagctg
     1321 ctcgcatagt cgagggtgcc gccgttcgga gccagggcat aggccatgtc ggaccagggc
     1381 acgatcgggg cggcccatgg agtagagaat gcctcccatg ctgttga
//
LOCUS       DAOQMF010000603         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_74596_length_1426_cov_2.215901, whole genome shotgun sequence.
ACCESSION   DAOQMF010000603 DAOQMF010000000
VERSION     DAOQMF010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74596_length_1426_cov_2.215901"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLV77_09425"
     CDS             <1..837
                     /locus_tag="PLV77_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16110.1"
                     /translation="VFNLKRIVGLVCLACVPALVGVVGAGPASAADETINLRTVVVPA
                     HGKVFKRRSVPAKMQLSATVNTPASSLKVNPLKRAIIQFPKGLTFHPNNRKTPVCSDK
                     ALSETSNLAAGPAGIVELCPKSVIGTGTAKIYLAKVHEDSALVSDPQLVIFNAGVAKG
                     GLAKMKIYAYSRTTNVGILMHGTVSKKGIINVFIPVLSNDSATASFILAIPGPGIKDG
                     GKTIKGRDANYARIRCASGKWVTRGTFTLGERSYPAGTPTGPSTTVKAKPSTVKCHGA
                     RG"
     gene            999..>1426
                     /locus_tag="PLV77_09430"
     CDS             999..>1426
                     /locus_tag="PLV77_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16111.1"
                     /translation="MSHSKKVAGTFSALLVLALPLAGTAFAASETITLTTTTTPLSGK
                     LFKEVRVPVNSSLSVEVHTPDDAPKVNPLKRSVMKFPTDLTYNPNNKVTPVCTDSQLS
                     DQSNLAAGVAGVVGLCPDSVVGTGTAAIYLAKVNLPTALIT"
BASE COUNT          285 a          455 c          430 g          256 t
ORIGIN      
        1 gtgtttaacc tgaagcgaat cgtcgggctg gtctgtctgg cttgcgtgcc ggcacttgtg
       61 ggtgtcgtgg gcgctggtcc cgcgtcggcc gcagacgaaa cgatcaacct gaggaccgtc
      121 gtggtacctg cacacgggaa agtattcaag cgccgcagcg tgccggcaaa aatgcagctc
      181 tcggcaacgg tcaacacccc ggcttcttcg ctcaaggtca atccgctgaa gcgggcgatc
      241 attcagtttc ccaaggggct gaccttccat ccgaacaacc ggaagactcc ggtctgttcc
      301 gacaaggccc tcagcgagac ctcaaacctt gccgccgggc cagccggaat tgtcgagctc
      361 tgcccgaagt cggtgatcgg caccggtacc gccaagatct acctggccaa ggtgcacgag
      421 gattcagcgc tggtctcgga tccgcagctg gttatcttca atgccggcgt tgccaagggc
      481 ggcctggcca agatgaagat ctacgcctac tcgaggacca ccaatgtcgg catcctgatg
      541 cacggcacgg taagcaagaa gggcatcatc aacgtcttca tcccggtgct cagcaacgac
      601 tcggcgactg ccagcttcat tctggccatc ccgggccccg gaatcaagga tggtggcaag
      661 accatcaagg gtcgtgacgc caactacgcc cggatccgtt gtgcatctgg caagtgggtg
      721 acccgaggta ccttcaccct gggtgagcgg tcctacccgg cgggcacccc gaccggcccc
      781 tcgaccacgg tcaaggccaa gccttcaacc gtcaagtgtc acggcgctcg aggctagggc
      841 agttgagcct ctgaggaggc agatcaattg ctgaaacggg ccggggactg gcaacagtct
      901 ccggcccgtt tgtcgttcgc tgccaatcat cccccggcag gctgtaaagt ccaagatgag
      961 tcgtcatggg aggcgattca ctgaacggga ggggtcacat gtcgcactcg aagaaagtgg
     1021 cgggaacatt cagcgccctg ctggttctgg cgctgccttt ggccggcacc gccttcgcgg
     1081 caagcgagac gatcaccctg accacgacca cgaccccgct ctccggcaag cttttcaagg
     1141 aggttcgggt accggtcaat tcctcgctct cggtcgaggt ccacaccccg gatgatgccc
     1201 cgaaggtgaa cccgctgaag cggtcggtga tgaagttccc gaccgatctg acctacaacc
     1261 cgaacaacaa ggtcaccccg gtctgcacgg acagccagtt gagcgaccag tccaacctgg
     1321 cggctggagt ggccggggta gtcggccttt gccccgattc ggtggtcggg accgggacgg
     1381 cggcgatcta cctcgccaag gtcaacctgc cgacagcgct catcac
//
LOCUS       DAOQMF010000604         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_75384_length_1418_cov_3.232575, whole genome shotgun sequence.
ACCESSION   DAOQMF010000604 DAOQMF010000000
VERSION     DAOQMF010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75384_length_1418_cov_3.232575"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLV77_09435"
     CDS             complement(<1..476)
                     /locus_tag="PLV77_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC16112.1"
                     /translation="MRVVVADDSILLREGVVRVLEDDGFEVVGQAGDAEDLLRKVSAH
                     KPDVAVVDIRMPPNLTDDGLQAAKKIRAEHPDTGVLVLSQYLEESYALDLVADSAEGV
                     GYLLKDRVAEIDRFTESVRRVGSGGSALDPEVVSHLLGRRRREDPLEVLTVREREVL"
     gene            complement(467..>1418)
                     /locus_tag="PLV77_09440"
     CDS             complement(467..>1418)
                     /locus_tag="PLV77_09440"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC16113.1"
                     /translation="GRMLERVAHESDPVKMQEAVAEALGDPSVEIAFWLPDTAGYVDA
                     RGLPIAEPDPDSNRAASQVTMDGRRVALIIHDGALLEEPEKIEAVSRGAALAFENARL
                     EAEVRANVAELRASRTRIVEAGDAARREIERNLHDGAQQNLVSLALKLQLARSRVESD
                     PRGAIEIIDGASAELEQTLAELRELARGIHPAVLSDRGLDAALLALATRAPLPVEVGE
                     MPDDRLPEPVEAAAYYVVAESLTNVARYSEASRAKVDITRENGVVRIRIADDGVGGAD
                     PARGSGLKGLADRIAALDGHFMLASPDGLGTIVEAEIPCG"
BASE COUNT          204 a          491 c          496 g          227 t
ORIGIN      
        1 aggacttcac gttcacggac cgtcaggacc tcgagcgggt cttcgcgccg gcggcggccg
       61 agcaggtggg agaccacctc ggggtcgagg gccgagccgc cgctgccgac ccggcggacc
      121 gactcggtga agcgatcgat ttcagcgacc cggtccttca gcaggtaacc gaccccttcg
      181 gccgagtcgg cgaccaggtc gagggcgtag ctctcctcca gatactggga aagcacgagg
      241 acaccggtgt ccgggtgttc ggcccggatc ttcttggccg cctgaagccc gtcgtcggtc
      301 aggttcggcg gcatgcggat gtcgaccacc gcgacatcgg gcttgtgggc actgaccttg
      361 cggagaaggt cttcggcgtc acccgcctgg ccaaccacct cgaaaccgtc gtcctcgagc
      421 acgcggacca ctccttcgcg cagcaggatc gagtcgtcgg cgacaactac ccgcacggga
      481 tctccgcctc cacgatcgtg cccagaccgt ccgggctggc cagcatgaag tgcccgtcca
      541 gggcggcgat ccggtcggcc aggcccttca gcccggaccc ccgggcgggg tcggctccgc
      601 cgacgccgtc gtcggcgatc cggatgcgga ccaccccgtt ttcccgggtg atgtcgacct
      661 tggcccggct tgcctcggaa tagcgggcga cgttggtcag cgactcggcg accacgtaat
      721 aggcggcggc ctcgaccggt tccggcaggc gatcatccgg catttctccg acctcgaccg
      781 gcagcggagc gcgagtcgcg agggcgagca gggcggcatc caggccgcgg tcggaaagca
      841 cggcggggtg gatgccacgg gccagttcgc gaagctcggc cagggtctgt tcgagttcgg
      901 cgctggcgcc gtcgatgatc tcgatcgcgc cgcgggggtc ggactcgacc cgggaacggg
      961 ccaactgcag tttgagggcg agggagacca ggttctgctg ggcgccgtcg tggaggttcc
     1021 gttcaatctc gcggcgggcg gcgtccccgg cctcgacgat gcgggtgcgg gaagccctca
     1081 gttcggccac gttggcccgg acctcggcct ccagccgcgc gttctcgaaa gccagtgcgg
     1141 cgccacggct caccgcctcg atcttctccg gttcctcgag cagggcgcca tcgtggatga
     1201 tcagcgcgac ccttctgccg tccatcgtga cctggctcgc cgcccggttc gaatccggat
     1261 caggctcggc gatcggcagg ccacgggcgt cgacgtagcc ggcggtgtca ggcagccaga
     1321 aggcgatctc gaccgagggg tcgccgagcg cttcggcgac cgcttcctgc atcttcaccg
     1381 gatcgctttc gtgggccacc cgttcgagca tccggccg
//
LOCUS       DAOQMF010000605         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_75924_length_1413_cov_3.195140, whole genome shotgun sequence.
ACCESSION   DAOQMF010000605 DAOQMF010000000
VERSION     DAOQMF010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75924_length_1413_cov_3.195140"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLV77_09445"
     CDS             complement(<1..312)
                     /locus_tag="PLV77_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16114.1"
                     /translation="MPGTVTENAVSELRRVDAIRYVTALREGGSLPGLVEGDDDGTWV
                     VKFHGAGQGPVALVSEVVAGEIARRIGLPVPELVLVEVAPELGYAEPDPAPGLRPGGV
                     VA"
     gene            373..972
                     /locus_tag="PLV77_09450"
     CDS             373..972
                     /locus_tag="PLV77_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNC16115.1"
                     /translation="MSTLSNTVDSPRVPVLADLFANTWIKNAVLVLGGALFMALFQQI
                     SFSMEPFPAPITGGTLGVLLIGSALGSRRGAAALLLYLMMGLILPVYSEGREGAEVLF
                     NTASTGYFLGYLIAAYVVGYLAEHGRDRKFQTAFVSFLFCQLIIFACGVLGLMIVMNF
                     DFSTAFHDGFVLYIPIELIKAAIGAILLPAAWKLAEKTR"
     gene            1067..>1413
                     /locus_tag="PLV77_09455"
     CDS             1067..>1413
                     /locus_tag="PLV77_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HNC16116.1"
                     /translation="MLNILASYDLGDALLTTLAIFFFVIWIWIVITILMDIFRDHELS
                     GWWKAVWVIFLIFIPFLTALVYLIARGSGMRERAIKEQADAKKHFDEYVRETAQTTPV
                     DELHKLDQLRQSGG"
BASE COUNT          220 a          487 c          407 g          299 t
ORIGIN      
        1 cgccaccacc ccccccggtc gcaaacccgg agcggggtcc ggctcggcgt agccgagttc
       61 cggggcgacc tcgaccagca ccagttcggg caccggaagg ccgatccggc gggcaatctc
      121 cccggccacg acctcggaga ccagggcgac cgggccctgc ccggcaccgt ggaacttgac
      181 cacccaggtg ccgtcgtcat ccccctcgac caggcctggc agcgacccgc cctcgcgcag
      241 cgcggttacg tagcggatcg cgtcgacccg cctcagttca ctgaccgcgt tctcggtgac
      301 cgtgccgggc atcgctatag cttcgcatcc gggtaggctt cagccgttct tcacgcctaa
      361 gaaagggtct cgatgtccac gctttccaac acggttgact ctccccgtgt tcccgtcctc
      421 gccgatttgt tcgccaatac ctggatcaag aatgccgtcc tggtactcgg tggtgcgctc
      481 ttcatggcgc tgttccagca gatctcgttc tcgatggagc cgttcccggc cccgatcacc
      541 ggtggcaccc tcggcgtcct tctgatcggc tcggccctcg gctcccggcg tggcgctgcg
      601 gccctgctcc tctacctgat gatgggcctg atcctgccgg tctattccga aggccgggaa
      661 ggcgctgaag tcctcttcaa cacggcttcg accggttact tcctcggcta cctgatcgcc
      721 gcctacgtgg tcggctacct ggccgagcac gggcgggacc gcaagttcca gaccgcgttc
      781 gtctccttcc tcttttgcca gttgatcatc ttcgcctgcg gtgtcctcgg gctgatgatc
      841 gtcatgaact tcgatttctc gaccgccttc cacgacggct tcgtgctcta catcccgatc
      901 gagctgatca aggccgcgat cggcgcgatc ctgctgccgg cggcctggaa gctggccgag
      961 aaaacccgct gaatccctga tcacccgtcc ggggtgatga gggacgggcc gatcgggtgt
     1021 agcctgagcg tgttcgtccc cgtcttcttt cctgaaagga acagacttgc taaatatcct
     1081 ggcctcctac gaccttggtg acgcgcttct caccacgctc gcgatcttct ttttcgtgat
     1141 ctggatctgg atcgttatca cgatcctgat ggacatcttc cgtgaccatg agctctccgg
     1201 ctggtggaag gccgtgtggg tgatcttcct gatcttcatc ccgttcctga ccgcgctggt
     1261 ctacctgatc gcccgtggtt ccgggatgcg ggaaagggcg atcaaggaac aggctgacgc
     1321 caagaaacat ttcgacgagt acgttcggga aaccgcccag accacgccgg tcgacgagct
     1381 ccacaagctc gaccagctgc gtcagagcgg tgg
//
LOCUS       DAOQMF010000606         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_75929_length_1413_cov_3.081738, whole genome shotgun sequence.
ACCESSION   DAOQMF010000606 DAOQMF010000000
VERSION     DAOQMF010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75929_length_1413_cov_3.081738"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1413)
                     /locus_tag="PLV77_09460"
     CDS             complement(<1..>1413)
                     /locus_tag="PLV77_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC16117.1"
                     /translation="SQIGTAAAIYVAGAVLGALFFGQLTDRFGRKKLFLITLAVYITA
                     TIATAFSFSAWYFYLCRFFTGAGIGGEYSAINSAIDELIPARVRGRVDLIINGSYWLG
                     AAAAALGSIVLLDTALFAADVGWRLAFGLGATFGVAILIVRRHVPESPRWLFIHGREK
                     EADRIVSDIEREVERETGRQSLEEPEEMIEVEQRKTISLGEVASTAFRKYPKRTALGL
                     ALFVGQAFLYNAITFDLGTLLGTFYGVASGTVPIFIAVYALSNFAGPLVLGRLFDTVG
                     RKPMIVLTYGIAAAGGLALGLLFTQDGFDKYWFMALLVTIFFFASAGASSAYLTVSEI
                     FPMETRALAIAFFYAVGTGLGGIVGPLLFGHLIETGDRSTMAIGFYLGAGIMLIGALA
                     ELIFGVKAEKQPLEQIARPLTATSDQVEAESGPKRELPRRRRTGRFRPGPGTLVMARG
                     YPLTAGDEVEAAVEVEALVAI"
BASE COUNT          271 a          449 c          471 g          222 t
ORIGIN      
        1 aatcgcgacc agcgcctcca cttcaaccgc ggcctcgacc tcgtcaccgg cggtaagcgg
       61 gtagccacgg gccatcacga gcgtgcccgg gccgggccgg aagcggccgg tccgacggcg
      121 gcggggaagt tcgcgttttg gaccgctttc ggcctccacc tgatccgaag tggcggtcag
      181 cggcctggcg atctgctcta gcggctgctt ttcggctttg accccgaaga tcagttcggc
      241 cagggcaccg atcagcatta ttccggcgcc gagatagaag ccgatcgcca tcgtgctccg
      301 gtcaccggtt tcgatcaggt ggccgaagag caacgggccg acgatcccgc ccaggccggt
      361 accgaccgcg tagaagaagg cgatcgccag ggcccgggtc tccatcggaa agatctcgct
      421 caccgtcagg taggccgagc tggccccggc cgaggcgaag aagaagatcg tgaccagcaa
      481 cgccatgaac cagtacttgt cgaagccgtc ctgggtaaac agcaggccga gggcaagtcc
      541 gccggcggcg gcgatcccgt aggtgagcac gatcatcggc ttgcggccta ccgtgtcaaa
      601 gaggcgaccc agaaccagtg gcccggcgaa attgctcagg gcatagaccg cgatgaagat
      661 cggcacggtg ccggaggcaa caccgtagaa cgtgccgagc agggtcccga ggtcaaaggt
      721 gatcgcgttg tagagaaagg cctggccgac gaacagggcg agccccaggg ctgttcgttt
      781 cgggtacttg cgaaaggcgg tcgaggcgac ctccccgagg gagatcgtct tgcgttgttc
      841 cacctcgatc atctcttccg gctcctccag cgactggcgg ccggtttcgc gttcgacttc
      901 ccgttcgatg tcgctgacga tccggtcggc ttccttctcc cgtccgtgga tgaacagcca
      961 gcgagggctc tcgggcacat gcctgcgaac gatcaggatc gcgactccga aggtggcgcc
     1021 gaggccgaag gcgagcctcc accccacgtc ggcagcaaac agggccgtgt ccagcagaac
     1081 gatcgaaccc agcgcggctg ccgccgcccc cagccagtag ctgccgttga tgatcaggtc
     1141 gacccttccc cgcacccggg ccgggatcag ctcgtcgatc gccgagttga tggctgagta
     1201 ttcaccgccg attccggcgc cggtgaagaa ccggcagagg tagaaatacc aggcggaaaa
     1261 cgagaaggcc gtagctatgg tggcggtgat gtagacggcc agcgtgatca ggaagagctt
     1321 cttgcggccg aagcgatcgg tgagctggcc gaagaagagc gccccgagca ccgctcccgc
     1381 gacgtagatc gcggcggcgg tgccgatctg gct
//
LOCUS       DAOQMF010000607         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_77235_length_1401_cov_3.333581, whole genome shotgun sequence.
ACCESSION   DAOQMF010000607 DAOQMF010000000
VERSION     DAOQMF010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77235_length_1401_cov_3.333581"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1401)
                     /locus_tag="PLV77_09465"
     CDS             complement(<1..>1401)
                     /locus_tag="PLV77_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16118.1"
                     /translation="SSNRVVPGDLTGPYLPRAQWQFVDDDISTVDHFHPGLSGQRKMA
                     QAAWESGYNFTDSTMPTGAASISPAPLQSGTSFLPSTVSTTWSDAAGIKGIQYRTHFN
                     GTASAWQTQLGTTTIPVNQTETYGYLTQKTFSVTVTTPGTTYVESRAMDVNGNLSASK
                     VTEVNYDPNAIAAPQITSTPPSITASNAASFSFTGAISGTGYECSLDGGAFSDCSPPK
                     SYSSIADGQHEFRVRLKSAVASGPAVNYQWQIDSSAPPAPTIHTGPAANTKLTVADFT
                     FSGTEAALECSLDGATFASCTSPRNVFVPTDGVHRFEVRQKTITGVPGTSAVYTWTVD
                     NTKPPAPVFGEGPSSPTNSTSASIQFTGEPGGTYSCQLTGPGGLQINNCSSPRNLAGL
                     AQGEYTLSVSQKDQLGNQGPAASYSWTIDLTAPVAPQVGSGPPARTASRDATVTFTGE
                     AGGHFECALDDADFFF"
BASE COUNT          222 a          392 c          537 g          250 t
ORIGIN      
        1 cagaaaaaga agtccgcatc gtcgagggcg cattcgaagt gaccgccggc ctccccggtg
       61 aaggtaaccg tggcatcgcg gctcgcggtc cgggccggtg gtccggaacc gacctgcggc
      121 gccaccgggg cggtcaggtc aatcgtccag gagtagctgg cggccgggcc ctgatttccg
      181 agctggtcct tctgggaaac cgagagcgtg tactcgccct gcgccaggcc ggccaggttg
      241 cgggggctgg agcagttgtt gatctggagc ccgcccgggc cagtgagctg gcagctgtag
      301 gtcccgcccg gttcgccggt gaactggatc gaagcggagg tcgagttggt cggggaggag
      361 ggaccttcgc cgaaaaccgg ggcggggggc ttcgtgttgt cgaccgtcca tgtgtaaacg
      421 gcggaggttc ccgggactcc cgtgatcgtc ttctgcctga cttcgaaccg gtggaccccg
      481 tcagtcggga cgaagacatt ccggggcgag gtgcaggaag cgaaagtagc gccgtcgagc
      541 gagcactcga gggcggcttc ggtgcccgag aaggtgaaat cggcgaccgt cagtttggtg
      601 ttggcggcag gaccggtgtg aatcgtcggc gcgggagggg ccgaggagtc gatctgccac
      661 tggtagttga cggccggccc ggaggcgacc gctgacttca ggcggacccg gaactcgtgc
      721 tggccgtcag cgatcgacga gtaggacttg ggtggcgagc agtcgctgaa cgcgcccccg
      781 tcaagcgagc attcgtagcc ggtgccgctg atcgcgccgg taaacgagaa gctcgccgcg
      841 ttcgaggcgg tgatcgaggg cggggtcgaa gtgatctgcg gcgcggcgat cgcgttcggg
      901 tcgtagttga cctcggtgac cttcgaggcg ctgaggttgc cgttcacgtc catcgcccgg
      961 ctctcgacat aggtcgtgcc cggggtagtg acggtgaccg agaaggtctt ctgggtcagg
     1021 tatccgtagg tctcggtctg gttgaccggg atcgtcgttg tgccgagctg ggtctgccag
     1081 gcgctggcgg tcccgttgaa atgggtccgg tactggatcc ccttgatccc ggctgcatcc
     1141 gaccaggttg tgctgaccgt ggacggcagg aaactggtcc ccgactggag cggcgccgga
     1201 ctgatcgacg cagcgccggt tggcatggtc gagtcggtga agttgtagcc ggactcccag
     1261 gctgcctggg ccatcttgcg ctggccggaa aggccgggat ggaaatggtc gacggtgctg
     1321 atgtcatcat cgacgaactg ccactgggcg cgaggcaggt agggaccggt gaggtccccg
     1381 gggacgaccc ggttggatga g
//
LOCUS       DAOQMF010000608         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_77980_length_1394_cov_3.126214, whole genome shotgun sequence.
ACCESSION   DAOQMF010000608 DAOQMF010000000
VERSION     DAOQMF010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77980_length_1394_cov_3.126214"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLV77_09470"
     CDS             complement(<1..406)
                     /locus_tag="PLV77_09470"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNC16119.1"
                     /translation="MEIERSQPTRFGEARRTPGAHGYVAYFPAPLVRSIELPSSTVNL
                     LADTEASLGELSAVGRLLPNPDLLIRPYMLREAVSSTRIEGTQAEMSEVFDVDAARSD
                     PNPDVEEVVNYVSAMNWGLAQLDRLPLSSRLIL"
     gene            complement(483..>1394)
                     /locus_tag="PLV77_09475"
     CDS             complement(483..>1394)
                     /locus_tag="PLV77_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC16120.1"
                     /translation="HQGSPGNELSVFGQEKTGETVKLAKMNLAIHGLSGDVREGNSYY
                     EDPHESHGRFDFVMANPPFNVDKIDKAKLEDDPRFPLLPKADNGNYIWIQLFANSLNE
                     QGRAGFVMANSASDARHSEAEIRQRLIEDRLVDVMVAVSPNFFYTVTLPVTLWFLDRG
                     KKGGEREDQVLFIDARQVFNQIDRAHRDFTTEQLEFLANIVRLYRGEDPEFAEDKNEL
                     FDERFPEGEFADVTGLCKVATIEEIKAQGWSLNPGRYVGTEVEELEDEVFEEKLAEAQ
                     AELRVLGAQARELEEGVDAVLTQLLEK"
BASE COUNT          280 a          419 c          402 g          293 t
ORIGIN      
        1 ccagaatcaa tcggctgctg agcggcaggc ggtcaagctg ggccagaccc cagttcatgg
       61 ccgagacgta attgacgacc tcctcgacat cagggttagg gtcggaacgg gcggcatcga
      121 catcaaatac ttctgacatt tcagcctggg tcccttcgat ccgggtcgag gagactgctt
      181 cccgcaacat gtagggacgg atgagaaggt cagggttcgg caggaggcgg ccgaccgcgg
      241 atagttctcc caaagacgct tcggtatcag ccagaagatt gacggtcgaa gacggcagct
      301 cgatcgacct gaccagcggc gcagggaaat acgccacgta gccgtgagca cccggggtgc
      361 gccgggcctc cccgaaacgc gtcggctggg aacgttcgat ctccatcaac gaatcctagc
      421 ctctctaacg aatctcatgt gaaacgtaat ggtcagagat cgaccctaac gtcggtgaaa
      481 ggctacttct cgagaagttg ggtcagcact gcatctaccc cttcttccag ctctctcgcc
      541 tgggctccga gaaccctcaa ctcagcctgg gcctcggcca gcttctcctc gaacacttca
      601 tcctcaagtt cttcgacctc ggtccccacg tatcggcccg ggttgaggct ccagccctgg
      661 gccttgatct cctcgatcgt cgcgaccttg cagagtccgg tcacgtctgc aaattcacct
      721 tccgggaaac gctcatcgaa gagctcgttc ttatcctctg caaattccgg gtcttcgccc
      781 cggtaaaggc gaacgatgtt ggccagaaac tccagttgct cggtagtgaa atcacggtgg
      841 gcacgatcga tctggttgaa gacctggcga gcatcgatga agagaacctg gtcttcccgt
      901 tcgccgccct tcttgccccg gtcgagaaac cagagagtta ccggaagggt gacggtgtag
      961 aagaagttcg ggctgacagc caccatcacg tcgaccagtc ggtcctcgat cagtcgctgg
     1021 cgaatctctg cctcgctgtg acgggcatcg gaggcagaat tagccatcac gaatccggcc
     1081 cgaccctgct cgttgagcga attggcaaat agctggatcc agatgtagtt gccgttgtca
     1141 gctttcggca gcagcggaaa gcgcgggtca tcttccagct tcgccttgtc gatcttgtcg
     1201 acgttgaacg gtgggttggc catcacgaaa tcgaagcgcc cgtggctttc gtgcgggtcc
     1261 tcgtaatagc tgttgccctc acgaacgtcc ccggagagcc cgtggatcgc gaggttcatt
     1321 ttcgcgagct tgaccgtctc gccggtcttc tcctggccga agacggagag ttcgttgccc
     1381 ggagacccct ggtg
//
LOCUS       DAOQMF010000609         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_77986_length_1394_cov_2.962659, whole genome shotgun sequence.
ACCESSION   DAOQMF010000609 DAOQMF010000000
VERSION     DAOQMF010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77986_length_1394_cov_2.962659"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..215
                     /locus_tag="PLV77_09480"
     CDS             12..215
                     /locus_tag="PLV77_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC16121.1"
                     /translation="MPTGTVKWFSDEKGFGFITPDDGSKDVFVHHTAIQSDGFRTLAE
                     GAKVSYEAEDGPKGPAAASVEAI"
     gene            392..>1394
                     /locus_tag="PLV77_09485"
     CDS             392..>1394
                     /locus_tag="PLV77_09485"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16122.1"
                     /translation="MKALVTFAATAAVLFSASALAKAAPGSVNPDFGKNGFVRVSTSP
                     GQKTIVRDMIRTASGNLLLSGGAGVAGNGFLLRLLPDGSRDPEFGNGDGLVLTPASQW
                     AKLELQGERIVAFGRSGSDPVVARFESDGTLDESFGDQGTVLLDVRSRFGEDADQVLE
                     TPGPGIDSEGRIVAGSTVASCATTRIEPDHGRHRCGNVVLFRFSPDGQPDTDYGNGGV
                     VMLGAGWNAGGRSAALALDGQDRVLVRTRDSVYYGSPQRTRNDVLQRFLPSGAYDHSF
                     GADGIVDLKVWAGEPGVANRGRILEGPDDRISVAANFFARVGRSGQVLSRPRTGSVDS
                     "
BASE COUNT          251 a          461 c          457 g          225 t
ORIGIN      
        1 tggaggacta aatgcctact ggcacagtca agtggttcag tgacgaaaag ggcttcggct
       61 tcatcacgcc ggacgacggt tcgaaggacg ttttcgtcca tcacaccgcc atccagtcgg
      121 acggcttccg caccctcgcg gaaggcgcca aggtgagcta cgaggcagag gacggaccca
      181 agggtccggc cgccgcttcg gtcgaggcca tctaggtcga ctgaccacgg ggcgcttcgc
      241 gccccggaga agcaacgcga gcgcgcccgc cacccggcgg gcgtttcgct ttaagcgacc
      301 gcacctgagc gggctgaccg caacccggtg gccgacaacg ggttatctgg tcatcgcgga
      361 ccggaatccg cactaccccc cgggagaaaa aatgaaggca ctggtcacgt tcgccgccac
      421 cgcggctgtc ctgttttctg cttcggcgtt ggccaaggcc gcgccaggct cggtcaaccc
      481 cgacttcggc aagaacggat tcgtgagggt ttccacgagt ccgggccaga agaccatcgt
      541 cagggacatg atccgcaccg cttccggcaa tctccttctt tccggaggcg ctggcgtggc
      601 ggggaacggg ttcctgctcc ggttgctgcc ggacggaagc cgggatccgg aattcgggaa
      661 tggcgacgga ctcgtcctga ctcccgcctc gcagtgggcg aagctcgaac tccagggcga
      721 gaggatcgtc gccttcgggc gttccggttc agatcccgtc gttgcgagat tcgaatcgga
      781 cggcaccctc gacgagagct tcggcgacca gggaaccgtg ctgctggacg tgaggtcccg
      841 gttcggagag gacgccgatc aggtgctcga aaccccggga ccgggaatcg acagcgaagg
      901 caggatcgtg gccggctcca ccgtcgcaag ctgcgcgacc acccggatcg agccggacca
      961 cggccgacac cgctgcggca acgtcgtcct tttccggttt tctccggacg gtcaacccga
     1021 cactgactac ggcaacggcg gggtcgtgat gctcggggca ggctggaatg ccggaggcag
     1081 gtcggctgcg ctcgcgctcg acggccagga tcgcgttctg gtccgaaccc gcgacagcgt
     1141 gtactacgga agcccccaaa gaacccgcaa cgacgtgctc cagcggttcc tgccatccgg
     1201 cgcatacgac cacagcttcg gcgcggatgg catcgtcgat ctcaaagtct gggccggcga
     1261 gccgggagtc gccaaccgcg gcagaatcct cgaaggaccc gacgaccgca tctcggtcgc
     1321 tgccaatttc ttcgcaaggg tggggcggtc cggtcaagtc ctgtcccggc cccgaaccgg
     1381 ttccgtcgac tcgc
//
LOCUS       DAOQMF010000610         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_78152_length_1392_cov_3.871354, whole genome shotgun sequence.
ACCESSION   DAOQMF010000610 DAOQMF010000000
VERSION     DAOQMF010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78152_length_1392_cov_3.871354"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLV77_09490"
     CDS             complement(<1..269)
                     /locus_tag="PLV77_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16123.1"
                     /translation="MLVSLSLLLVWDGSAAASAARPEAFAEKVALPAVGEALAPRIAV
                     RARPISKAPVRAVMKAFRFDYRPTVFFAIDRRETGQGEWLKVRIPG"
     gene            complement(299..>1392)
                     /locus_tag="PLV77_09495"
     CDS             complement(299..>1392)
                     /locus_tag="PLV77_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16124.1"
                     /translation="TVVIDVSVNVLEGGEYTNCADVASPTPDPDGDNNHSCTTNPAEN
                     TDLAIEKTGPPYFPEGKNRNYTLLVTNVGDQPTGGTTTVTDRIPDVLRPEAAYGNGWS
                     CAISGQKVTCTRSDVLEPGDSFPLITIRVVARENLLFRPIGNTGHVHLPGDPNPDNDQ
                     DTVRTKKGAICEGGSLALNPAFVWVGQKTIVKATLRTRAGAPAIGLPVRIKGNGKGGA
                     SGKLRILTTNGKGKVHFTARATSTEARWTVSVPQSHQKAVLAPKRQETCRAMNVTPRT
                     IDINKTTVLTVRLRTPEGRPAIGITVVARGQGTGDSARTNGKGMARLRVRPNGQGYLV
                     VTAPNATRCRFRIGASDLDAAGAGNQLTG"
BASE COUNT          194 a          469 c          472 g          257 t
ORIGIN      
        1 ccggggatcc tgaccttgag ccactcgccc tggccggttt cccgccggtc aatcgcgaag
       61 aagacggtgg gccggtagtc gaaacgaaac gccttcatca ccgccctgac cggggccttc
      121 gatatcggtc tcgcccgcac cgcaatccgg ggggctagcg cctcaccgac cgccggtagc
      181 gccaccttct ccgcgaaggc ttcggggcgc gctgcgctgg ccgctgcgga gccatcccag
      241 acgagcagca gcgaaagaga aaccagcacc ccggttacta caccccgccg gaccatggct
      301 aaccggtcag ctggttaccg gcgccggcag cgtccaggtc actggccccg atccggaacc
      361 ggcaacgcgt ggcattgggc gcggtaacca cgaggtaacc ctgaccgttg ggacggaccc
      421 tgaggcgggc catgcccttg ccgttggtcc gagccgagtc acccgtgccc tgacccctgg
      481 cgaccacggt gatgccgatg gccggacggc cttcgggagt ccgcagacgg acggtcagca
      541 ccgtggtctt gttgatgtcg atcgtccgcg gggtcacgtt catcgcccgg caggtttcct
      601 ggcgcttcgg tgcgagcacc gccttctggt gactttgcgg cacgctcacc gtccagcggg
      661 cctccgtact ggtcgcccgg gcggtgaagt ggaccttgcc cttgccgtta gtggtcagga
      721 tccgcagctt gccgctggcg ccacccttgc cgttgccctt gatccggacc gggagcccga
      781 tcgccggagc tccggctctc gtccgcaggg tcgccttgac gatcgtcttc tggccgaccc
      841 agacgaaggc cgggttcagg gccagagacc cgccctcgca gatcgcgccc ttcttcgtgc
      901 ggacggtgtc ctgatcattg tccgggttcg ggtctcccgg caggtgcacg tggccggtgt
      961 tgccgatcgg ccggaagagc aggttttcgc gggcaaccac cctgatcgtg atcaggggga
     1021 atgaatcacc gggctcgagc acgtcggagc gggtgcaggt gaccttctgg ccgctgatcg
     1081 cgcagctcca gccgttgccg taggcggcct ccggcctgag cacatcggga atgcggtcgg
     1141 tcacggtggt ggtgcccccg gtcggctggt caccgacgtt ggtcaccagc agggtgtagt
     1201 tacggttctt gccctcgggg aagtacgggg ggccggtctt ctcgatcgcc aggtcggtgt
     1261 tttccgccgg gttggtggtg caggaatggt tgttgtcgcc gtccgggtcc ggggtcgggc
     1321 tggccacgtc ggcgcagttc gtgtactccc cgccttcgag cacgttcacc gacacgtcga
     1381 tcaccacggt ct
//
LOCUS       DAOQMF010000611         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_78175_length_1392_cov_3.130142, whole genome shotgun sequence.
ACCESSION   DAOQMF010000611 DAOQMF010000000
VERSION     DAOQMF010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78175_length_1392_cov_3.130142"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLV77_09500"
     CDS             <1..794
                     /locus_tag="PLV77_09500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC16125.1"
                     /translation="NTEFSPDLYGLACRKPTGLSAALRGTLATYSRVRLPNARALPGC
                     RPWTSFSRRSSAAQAGVHSTWPLTPVKDLPDESYWAKPPGCPLKGTPLSCEIDATMPR
                     YAMVMLGTNDIGMDIAFGIQPGSRIVSRLGGVVRALLARKVVPVLSTIPPIIRPDGTR
                     QGEVTAGVARSNAGIFELARKWHLPMINLWRALKEPSMIDLGLAGDGLHLSVAGAGGE
                     MVGVQPGASTFADSVDFTAAGLRYGANRRNLIWLKTLARLDRVTG"
     gene            796..>1392
                     /locus_tag="PLV77_09505"
     CDS             796..>1392
                     /locus_tag="PLV77_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16126.1"
                     /translation="MGRRFTAVLLAGLILLAGCGSSGEDSGQAPDPSRAPARAGESAR
                     TPQPVADLSSGSVLPDLSGAAGDRLRREVARADERKLNPDVFAKVGDSNTEWPQNLYG
                     LGCREVGYGSNGQLEAGVKKYSKGRFPGLVSLDGCSPVNSLTRRSAAAVSGVWTEWLM
                     TPTEDLYATEVGPPTDECRPEQTPLDCEIELIRPRWALI"
BASE COUNT          201 a          505 c          466 g          220 t
ORIGIN      
        1 cgaacaccga gttctccccc gacctctacg gcctcgcctg ccggaagccg accggtctga
       61 gcgccgccct tcgcggcacc ctcgccacct acagccgcgt gcgcctgccg aacgccaggg
      121 cgctgccggg ctgccggccg tggacctcgt tctcccgccg ctcctcggcc gcccaggccg
      181 gcgtgcactc gacctggcct ctaaccccgg tcaaggatct tcccgacgag agctactggg
      241 ccaagccgcc cggctgcccg ctcaaaggca ctccgctgag ctgcgagatc gacgcgacca
      301 tgccccgcta cgccatggtc atgctgggca cgaatgacat cggcatggac atcgctttcg
      361 gcatccagcc tggcagcagg atcgtcagcc gcctcggcgg cgtggtcagg gccctgctcg
      421 cccggaaagt ggttcccgtg ctgagcacga tcccgccgat catccggccg gacggcacca
      481 ggcaaggcga ggtcacggcc ggtgtggccc ggagcaacgc cggcatcttc gaactcgccc
      541 ggaagtggca cctgccgatg atcaatctct ggagggccct gaaggagcct tcgatgatcg
      601 acctcggcct ggccggggac ggcctccacc tcagcgtcgc gggggcaggc ggggagatgg
      661 tcggtgtcca gccgggagct tcgaccttcg ccgactcggt tgatttcact gcggccggcc
      721 tgcgctacgg ggcaaaccgc cgcaacctga tctggctgaa gaccctggcc cggctcgacc
      781 gggtcaccgg ttgacatggg ccgccgcttc acagccgtcc tcctggccgg gctgatcctg
      841 ctggccggtt gcggctcctc cggtgaggat tccgggcaag ctcccgaccc ctcccgggca
      901 cctgcccggg ccggggaatc ggcgcggacc ccgcaaccgg tggccgacct ttcgtccggc
      961 tcggtgctcc cggacctctc gggggcggcc ggtgaccgcc tgcgccggga ggttgcccgg
     1021 gccgacgaac ggaagctgaa cccggatgtc ttcgccaagg tcggcgattc gaacaccgag
     1081 tggccgcaga acctttacgg gctcggttgc cgcgaggtcg gttacggctc gaacggccag
     1141 ctcgaagcgg gggtgaagaa gtactcgaag gggaggtttc cgggcctcgt ctcgctggac
     1201 ggctgctcac cggtcaattc gcttacccgc cgttcggcgg ccgcggtttc cggggtctgg
     1261 accgagtggc tgatgacccc gaccgaggat ctctacgcga ccgaggtcgg ccctccgacc
     1321 gacgagtgcc ggccggagca gactccgctc gactgcgaga tcgaacttat ccggccccgt
     1381 tgggcgctga tc
//
LOCUS       DAOQMF010000612         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_78318_length_1391_cov_2.340569, whole genome shotgun sequence.
ACCESSION   DAOQMF010000612 DAOQMF010000000
VERSION     DAOQMF010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78318_length_1391_cov_2.340569"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLV77_09510"
     CDS             <1..218
                     /locus_tag="PLV77_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC16127.1"
                     /translation="FAQYNANYVGGDIVAGANTPRQMVFRPRVTLNPYKVTEGIWICS
                     AATPPGGGVHGMGGANAARSILSTLGS"
     gene            complement(284..>1391)
                     /locus_tag="PLV77_09515"
     CDS             complement(284..>1391)
                     /locus_tag="PLV77_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16128.1"
                     /translation="QFGKLLGRIEGEVAVGWLEEFTGGKALQGAKYIRDTRIAGGVAT
                     ETAEVATSASRLTGAGAKALPDAPSLGNLSNKQIDRFKQIVKNVNEKFGVKVEIQARP
                     INEYAAKVKGGIGKVEAVPTKNLTPDDVILGAPPEWVGQAGYYPPKLPKNFNKLDAAE
                     KARLQLRFDEKTKEFKQFKGVIEDPTGKTAKVHKALKKETEFDLGKNGKLKMELEATT
                     HKSGAVLISYKKLSVNGKPVFTGKPRPIISDVDMNAVVDAATGRHLPAGIRGQVELEV
                     MRQFAKAAEEGVFPFGYHGWTHSGFDIAAADFRHILKYQLMYMNEKDAIKLAKKYAAI
                     YGIDPSEFLDGYTRGKLLVKITATDAVLGPGAGL"
BASE COUNT          212 a          424 c          449 g          306 t
ORIGIN      
        1 atttcgccca gtacaacgcc aattacgtgg gcggcgacat cgtcgccggg gcgaacaccc
       61 cgaggcagat ggtcttccgg ccccgggtca cgctgaaccc gtacaaggtc accgagggaa
      121 tctggatctg ctccgccgcg accccgcccg gcgggggagt ccatggcatg ggcggagcca
      181 acgcggcccg ctcgatcctc agcacgctcg gtagctgaga agcatctggg ttgaagttat
      241 tcgttccatg tgcggataac ttcaacgtag atgcaggttc agcctagagg ccggcgcccg
      301 ggccgaggac ggcgtcggtg gcggtgatct tgaccaggag tttgccgcgg gtgtagccgt
      361 cgaggaactc ggacgggtcg atgccgtaga tcgcggcgta cttctttgcc agcttgatcg
      421 cgtctttctc gttcatgtac atgagctggt acttgaggat gtgacggaag tcggcggcgg
      481 cgatgtcgaa tccggagtgg gtccagccgt ggtaaccgaa ggggaagaca ccttcctcgg
      541 cggccttggc gaattggcgc atcacctcca gctcgacctg gccgcgaatg ccggcgggca
      601 ggtgacggcc ggtggccgca tcaaccactg cgttcatgtc cacgtcggag atgatcggcc
      661 tgggcttgcc ggtgaagacc ggcttgccgt tgaccgagag cttcttgtag ctgatcagca
      721 ccgccccgga cttgtgggtg gtcgcttcga gttccatctt cagcttgccg ttcttgccca
      781 gatcgaactc ggtttccttc ttcagggcct tgtgaacctt tgccgtcttg cccgtgggat
      841 cctcgatgac gcccttgaac tgcttgaact ccttcgtctt ttcgtcaaag cggagctgga
      901 ggcgggcttt ctcggccgcg tcgagtttgt tgaagttctt cgggagcttc ggcgggtagt
      961 aaccggcctg gccgacccat tcgggcggag ccccgaggat cacgtcgtcc ggggtcagat
     1021 tcttggtcgg gaccgcctcg accttgccga ttccgccctt gactttggcg gcgtactcgt
     1081 tgatcggccg ggcctggatt tcgaccttga ccccgaactt ttcgttgacg ttcttgacga
     1141 tttgcttgaa gcggtcaatc tgcttgttgc tcaggttgcc gagcgaaggt gcgtccggga
     1201 gggccttggc acccgcgccg gtgagacggc tggcgctggt cgcgacctcg gcggtttccg
     1261 tggcgacacc gccggcgatc cgcgtatccc gaatgtactt cgccccctgg agggccttgc
     1321 cacccgtgaa ctcctcgagc cagccgaccg ccacttcgcc ttcgatccgg ccaagcagtt
     1381 ttccgaactg g
//
LOCUS       DAOQMF010000613         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_78658_length_1388_cov_2.066017, whole genome shotgun sequence.
ACCESSION   DAOQMF010000613 DAOQMF010000000
VERSION     DAOQMF010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78658_length_1388_cov_2.066017"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1148)
                     /locus_tag="PLV77_09520"
     CDS             complement(12..1148)
                     /locus_tag="PLV77_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16129.1"
                     /translation="MRKLIYPLVLAAAWLGMAAAADAANESIGLSMEATPGPGGLYRE
                     VARPIGASLTVTVTVPSESPKVTPIKVANVTFPEDMDFFPNKEKTPPCPDSALNSQSN
                     LAAGIAAIVDLCPKSVIGTGSSVVKLAKNNKDSFANLTDPRLVIFNAGRNGKGRPKIV
                     IYGYSKSVNAGLLMHGTLASDGQLRIEIGVMPFDSAVSEFTLGIPGEDLEVPDDSAPG
                     GSRTVKGLDRAYLRGKCSTGTWLATGKFVLGERSDPSGVPVGEETLLSSNPFELPCEG
                     KAGHAKLAIRAIKGPRKIKARGKAGYVLTISNPGTATATNLRLGATGAVTGTGKTGSL
                     GPGESSKVRLPVVAGSRKGKGKIRFRVSANGSVRKESVRKILVG"
BASE COUNT          237 a          427 c          415 g          309 t
ORIGIN      
        1 gatgctccta tctacccgac gagaattttc ctaacacttt ctttacgaac tgagccgttc
       61 gcagataccc ggaaccggat tttccctttg cccttgcggg atcccgccac cacaggaagt
      121 cggactttgc tcgactctcc tggtccgagt gagcctgtct tcccggttcc ggtgaccgct
      181 ccggtggcgc cgagccgcag gttggttgcg gtggccgtgc ccgggttgct aatcgtgagg
      241 acatatcccg ctttgccgcg cgctttgatc ttcctcggtc ctttgatcgc ccttatcgcc
      301 agcttcgcgt gcccggcctt tccctcacag ggaagctcga aggggttgga agagagcagc
      361 gtttcttcgc cgaccggcac gccgctcgga tcggacctct ctcccagcac aaactttccg
      421 gttgccagcc aggtgccggt tgaacacttg cccctcaggt aagcccgatc aagccctttg
      481 acggtcctgc ttcctccggg ggcagaatca tcaggaacct ccaggtcttc gccgggaata
      541 ccaagcgtga attcgctgac cgcagagtca aacggcatta cgccgatctc gattctgagc
      601 tggccatcgc tggccagggt gccgtgcatc agcagtccgg cgttgaccga ctttgaatag
      661 ccgtagatga cgatcttcgg tcggcctttg ccgttccgac cggcgttgaa gatgaccaga
      721 cgggggtcag tgaggttggc gaacgaatct ttgttgttct tggccagctt gaccacagag
      781 gaaccggtgc caatgaccga tttcggacag agatcgacaa tcgcagcgat gccagcggcc
      841 aggttgctct gagagttcag ggcgctgtcg ggacagggcg gggtcttctc cttattcggg
      901 aagaagtcca tgtcctcggg gaaggtcacg ttggccacct tgatgggtgt caccttcggg
      961 ctctctgacg gcaccgtgac agtgaccgtg agactggccc cgatcggtcg ggcgacttca
     1021 cggtacagcc ctcccggacc gggagtggcc tccatcgaga gtccgatgct ctcgttggca
     1081 gcgtcagccg cggccgccat ccccagccag gccgctgcca gaaccagggg atagatcagc
     1141 tttctcatct ttaaacccat cgcgagagga aagcaaaagg gcggccccgg agggccgccc
     1201 ttcttgcgaa cttctgccag taggagagtc ctacttgacg gtcaccttga tggtggcctt
     1261 ggcggaggac ttaccgctct tggccgtgaa ggtcagcgtc ttcttgctgc ccttcctgcc
     1321 ggtgaccttg gccttgaccg tgacggtctt ggacgcgcca gggttgacat tgctggtcga
     1381 gcccttgg
//
LOCUS       DAOQMF010000614         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_79119_length_1384_cov_2.165538, whole genome shotgun sequence.
ACCESSION   DAOQMF010000614 DAOQMF010000000
VERSION     DAOQMF010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79119_length_1384_cov_2.165538"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PLV77_09525"
     CDS             <1..590
                     /locus_tag="PLV77_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC16130.1"
                     /translation="EKQPRGPGRPSTGARERVLDAAVETLLEEGYAGLTYAKVAVRAG
                     ENKSLISYYFGSKQGLVAAVADIIGERITVAVVSELNNVETVPDIAAGMIAGVWKVMD
                     EDPRLARLYFDLSAVSVVDDDVRDAIHLVKDRWREVIAGFMLDAGVRADRVAPLGTFL
                     MSGVQGLAIERLEGDQSDRLEAARKLFVRAAGAIA"
     gene            730..>1384
                     /locus_tag="PLV77_09530"
     CDS             730..>1384
                     /locus_tag="PLV77_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16131.1"
                     /translation="MALGAGAGSASAMTVTSTGESGCSLRVAIQAVAANDSGTACGAV
                     VSGGPTPINLPANTITPFDGQIVVPAGVNVQINGDNVNNPLTTVMDGTGATAGRIFEI
                     KSGASVKLSGLTVTGGQALNGAAPTSRFASYSGQDGGGILNGGNLTLDHVVLKGNHAG
                     DGGDGWNGALEESGGGAGWGGNGGGIYNSDGASLTITNSEISGNFAGQGGTGGSGGNG
                     "
BASE COUNT          233 a          444 c          483 g          224 t
ORIGIN      
        1 ccgaaaagca gccccgcggc cccggtcggc cttcgaccgg ggcccgggag cgcgtgctcg
       61 acgcggcggt cgagactctg cttgaagagg gctacgccgg gctgacctac gccaaggtcg
      121 cggtccgggc cggcgagaac aagtccctga tctcctatta cttcgggtcg aagcagggcc
      181 tggtcgccgc ggtcgccgac atcatcggtg aacggatcac cgtcgcggtc gtctccgagc
      241 tgaacaacgt cgagaccgtc cccgacatcg ctgccggcat gatcgccgga gtctggaagg
      301 tcatggacga ggacccccgc ctggcccggc tttacttcga tctcagcgcg gtctcggtgg
      361 tcgacgatga cgttcgtgat gcgatccacc tggtcaagga ccgctggcgc gaggtgatcg
      421 ccggcttcat gctggacgcc ggcgttcggg cggaccgggt cgcgccgctc gggaccttcc
      481 tgatgtcagg tgtccagggc ctggcgatcg agcggctcga gggcgatcag tccgatcgcc
      541 tcgaagcggc ccgcaagctt ttcgtccggg ccgccggcgc gatcgcctga ccggatcttc
      601 ccccttgaca taacccggtg aacctaagag agtcaagggt ggggttttcg cagggtccat
      661 atcgggaaga ggtacctcat agtgagcagg ttttcgtttc gtcttctggt ttccgtcatt
      721 tcgattggcg tggctctggg ggccggcgcc ggatctgcct cggcgatgac cgtcacctcg
      781 accggtgaga gcggctgctc gctgcgcgta gcgatacagg cagtggccgc gaatgacagc
      841 ggcaccgcct gcggggcggt ggtcagcggc ggtccgaccc cgatcaacct tccggccaac
      901 acgatcacgc cgttcgacgg gcagatcgtg gtgccggccg gagtcaacgt ccagatcaac
      961 ggcgacaacg tcaacaaccc gctgaccact gtgatggacg gaaccggcgc cacggcgggc
     1021 cggatattcg agatcaagag cggtgcgagc gtgaagctgt cgggcctgac cgtcaccggg
     1081 ggccaggccc tgaacggtgc cgcaccgacc agccgttttg cctcctactc gggccaggac
     1141 ggcggaggaa tcctgaatgg cggcaacctg acccttgacc acgtcgtgct caaggggaac
     1201 catgccggcg acggcggaga tggctggaac ggcgccctcg aagagtccgg cggaggcgcc
     1261 ggctggggtg gcaacggcgg cggcatctac aactccgacg gggcgagcct gaccatcacc
     1321 aactccgaga tcagcggcaa cttcgccggc cagggcggaa ccggcggttc cggagggaac
     1381 gggg
//
LOCUS       DAOQMF010000615         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_79550_length_1380_cov_2.393208, whole genome shotgun sequence.
ACCESSION   DAOQMF010000615 DAOQMF010000000
VERSION     DAOQMF010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79550_length_1380_cov_2.393208"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..335
                     /locus_tag="PLV77_09535"
     CDS             39..335
                     /locus_tag="PLV77_09535"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC16132.1"
                     /translation="MPGLIRGVARTAAIAGTATAVSNRVSRRQAAKWAEQDGPQYANP
                     RADMAQQQYQEPAPAPAAAPADPIAQLKELGELHQSGVLTDEEFAAQKAKILGS"
     gene            447..776
                     /locus_tag="PLV77_09540"
     CDS             447..776
                     /locus_tag="PLV77_09540"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNC16133.1"
                     /translation="MEARITDEEFEELIIRAIDRLPEQFREIINQIPVVISDQGRERH
                     AYGLYQGDGIARDNYPDRIIIFKDTLVRDFGHDPALLAAQVERTVRHELGHHLGYDEG
                     GVRGLGL"
     gene            822..1277
                     /locus_tag="PLV77_09545"
     CDS             822..1277
                     /locus_tag="PLV77_09545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC16134.1"
                     /translation="MSKATLNTNAGPITIEFFDDDAPKTVENFRKLAADGFYDGLTFH
                     RVIKDFMIQGGCPLGTGTGGPGYTFEDEFNDHGVVRGALAMANAGPNTNGSQFFIVTI
                     PSADWLNGKHTVFGEVTDGMDVVDAIEGTQTDGRDMPLDPQVIESVELA"
BASE COUNT          287 a          456 c          425 g          212 t
ORIGIN      
        1 cgaagcctag aaacctcaca gaagcaagga gaaccgaaat gccaggactg atcagaggcg
       61 tcgcccgcac cgcggcgatc gccggtaccg ccaccgccgt aagcaaccgg gtttcccgtc
      121 gccaggccgc caagtgggcc gagcaggacg ggccgcagta cgcgaacccc cgggccgaca
      181 tggcccagca gcagtaccag gagcccgccc cggcgcccgc cgccgccccg gccgacccga
      241 tcgcccagct caaggaactc ggcgagcttc accagagcgg ggttctgacc gacgaggaat
      301 tcgccgccca gaaggcaaag atcctcggca gctgacacaa acgcgcgatc cggtcttcgc
      361 tggatcgcaa caggaaccgt ccgatcacgg gatcaagaaa tggatggtcg gactcagcca
      421 gtagtcgact tcgcaatgat tcgggcatgg aggcccggat cactgacgaa gagttcgagg
      481 aactgatcat ccgggcgatc gaccggctgc ccgagcagtt ccgggagatc atcaaccaga
      541 ttccggtggt gatctccgac cagggccggg aacggcatgc ctacggtctc taccagggtg
      601 atggcatcgc cagggacaat tacccggacc ggataatcat cttcaaggac accctggtcc
      661 gtgacttcgg ccacgacccg gccctgctcg ccgcccaggt cgaacgcacc gtgcggcatg
      721 aactcggcca ccacctcggg tacgacgagg gtggtgtgcg gggcctcggg ctctgacccg
      781 gatcggcgag ccggagatcc atcggcctgt aggctcgacc catgtcgaaa gcaacgctga
      841 atacgaatgc cggtccgatc accatcgagt tcttcgatga cgacgccccc aaaacggtcg
      901 agaacttccg caagctcgcc gctgacggtt tctatgacgg actcaccttc caccgggtga
      961 tcaaggactt catgatccag ggcggctgcc cgctcggtac cggtactggt ggccccggct
     1021 acaccttcga agacgaattc aatgaccacg gagtggtgcg cggcgcgctc gcgatggcta
     1081 acgccggccc caacaccaat ggttcccagt tcttcatcgt caccatcccc tccgccgact
     1141 ggctgaacgg caagcacacg gtcttcggcg aggtcaccga cggtatggac gtggtcgacg
     1201 cgatcgaagg aacccagacc gacggccgcg acatgcccct ggatccccag gtaatcgaga
     1261 gcgtcgagct cgcttaactg cagtcgcgcc ggtctgtggc gtaagtggga tctcgcggtc
     1321 ctggaaccgg gttgacgtag ccgtgggggc tggtatgcat ctccgggaca ctcaggagta
//
LOCUS       DAOQMF010000616         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_79686_length_1379_cov_2.103474, whole genome shotgun sequence.
ACCESSION   DAOQMF010000616 DAOQMF010000000
VERSION     DAOQMF010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79686_length_1379_cov_2.103474"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLV77_09550"
     CDS             complement(<1..267)
                     /locus_tag="PLV77_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNC16135.1"
                     /translation="MSGLLALNFYSPVFVDQLKRGRKTATIRLGNKSAKYQRSQIVWV
                     TVGSRHSRREKIFSAVIDEVEVKRLSELSPRDIERDNPEFRRVEE"
     gene            complement(277..966)
                     /locus_tag="PLV77_09555"
     CDS             complement(277..966)
                     /locus_tag="PLV77_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC16136.1"
                     /translation="MTSAEESTVGLLRSVPLFAELDQDELEQFSRVAIPRAYPEGTRV
                     FHEGDHSDACYIVKSGTFRVTREHPDGRAITLATLTEGDIVGELAMLDGEVRSASVET
                     LGGDGELLALPASDVRALLERHPDITVKFVAALTRRLRAANERISRQSFQTVPSRVAG
                     VLSQLVAEEGEDGRQVTIRMNQADLAQLAGTSRESVSRFLADLERSGVVKAGRGQVTI
                     LEPARLGNYIF"
     gene            complement(1106..>1379)
                     /locus_tag="PLV77_09560"
     CDS             complement(1106..>1379)
                     /locus_tag="PLV77_09560"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNC16137.1"
                     /translation="FKSEACFEARVDPWRPATSLAKGAGGEDEAIRVLETARRQMQDA
                     VETGKRPGRIYKKTGRPCPRCRAARIRSRGQGEDNRTTYWCPNCQA"
BASE COUNT          225 a          443 c          454 g          257 t
ORIGIN      
        1 ctcctcgacc cggcggaatt ccgggttgtc gcgctcgatg tcccggggcg agagttcgga
       61 caggcgcttg acctcgacct cgtcgatcac cgcagagaag atcttttccc gcctggagtg
      121 ccgggaaccg accgtgaccc acacgatctg gctgcgctgg tacttggcgg acttgttgcc
      181 aagccggatc gtggcggtct tgcgaccccg cttgagctgg tcgacaaaca caggggaata
      241 gaagttgaga gccaggagtc cggacatttg cccgatctag aatatgtagt tgccgagccg
      301 ggcgggttcg aggatggtca cctggccccg ccctgccttc accacgcctg acctttccag
      361 atcagccagg aaccggctta cgctttcgcg ggaggtgccg gccagctggg ccaggtcggc
      421 ctgattcatc cggatcgtca cctggcggcc gtcctccccc tcctcggcga ccagctggga
      481 aaggaccccg gccacccgtg atggaacggt ctggaaggac tgccgggaaa ttcgttcgtt
      541 tgccgctcgc aggcgcctgg tcagcgcggc gacgaacttg acggtgatgt cgggatgcct
      601 ttcgagcagg gcccgcacgt cgctcgcggg cagggcgagc agctcgccgt caccgcccag
      661 ggtctcgacc gaggcggaac gcacctcgcc gtcgagcatc gccagttcac cgacgatgtc
      721 cccttcggtc agggttgcaa gggttatggc cctgccgtcc ggatgctcgc gggttacccg
      781 gaaggtgccg gacttgacga tgtaacaggc gtcggaatgg tctccctcgt ggaagaccct
      841 ggtcccttcg gggtatgcac gcggaatcgc gacacgcgaa aactgctcga gctcgtcctg
      901 atcaagctcg gcgaaaaggg gcacgcttct cagcagcccg actgtgctct cctcagccga
      961 agtcacccgc ggagtatttc gcatccgacc ggaaaaacaa aacgattctg atcaattgag
     1021 gtcagaaacc cttcccaggt gccttccggg ggcgtttacc caccaaaacg gtggccgatc
     1081 gcccccggtt tggacgggag tcggatcagg cctggcagtt cgggcaccag taggtggtgc
     1141 ggttgtcttc tccctggccg cgggagcgga ttctggccgc gcggcagcgg gggcaggggc
     1201 ggccggtctt tttgtagatg cggcccggcc gcttgccggt ctcgactgcg tcctgcatct
     1261 gccggcgggc ggtttcgagt acacggatcg cctcgtcctc gcctccggca cccttcgcca
     1321 gggaggtggc cggccgccag gggtcgaccc tcgcctcgaa gcaggcttcg gacttgaag
//
LOCUS       DAOQMF010000617         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_79992_length_1376_cov_3.666919, whole genome shotgun sequence.
ACCESSION   DAOQMF010000617 DAOQMF010000000
VERSION     DAOQMF010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79992_length_1376_cov_3.666919"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1376
                     /locus_tag="PLV77_09565"
     CDS             <1..>1376
                     /locus_tag="PLV77_09565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC16138.1"
                     /translation="GDAGAESAGGKALPESETKAGSSESVLRPPGSGTRDRTAREASK
                     GRTKGSRRIALPRLKGRKTKKAAGSGAAPTTAASGSATSRGKAATGNGNGQPLSIPSA
                     PGVGGNGNGTGNGNGNGTGLPPGVLTLPVPPPRPPKPKIKKLRVLFIVLGLGLLGFCS
                     AFFGMVMAVSQDLPAIENYAQFRTAKNSILVDSQGKYIGTLSNNENQMLLDAEQISPN
                     MKNAVVSIEDARFYEHRGIDFQGLGRALVQDIIARSAKQGASTITQQLVKQALEAQND
                     RSPLQKVREMSIAYHIEQQWTKDKILTEYLNTVYFGNGAYGIEAAARTYFGYAHPSCG
                     TDEEPCASLLTPAESALLAGIIQNPYGYDPKNDPDAALYRRNTVLDRMLDQGYITQEQ
                     HDEAVKEALPADDDIDPPSLDSKAPYFTSWMRQQLVDRYGAGRAFFGGLKVKTTIDTW
                     GGTTTLPD"
BASE COUNT          290 a          479 c          421 g          186 t
ORIGIN      
        1 caggtgacgc aggcgcagaa agcgccggcg gcaaggcgct gcccgaatcc gaaacgaagg
       61 cgggcagcag cgaaagcgtg ctccggccgc caggcagcgg cacccgtgac cgcacggcca
      121 gggaggcatc aaagggtcgc accaaaggtt cccgccggat cgcactgccc aggctgaagg
      181 gccggaagac caaaaaggcc gccggctccg gtgccgcccc gaccacggcc gcctcggggt
      241 ccgcgaccag cagaggcaag gccgcgaccg gaaacggaaa cgggcagccc ctctcgatcc
      301 cgagcgctcc cggggtcggc ggaaacggga acggaaccgg caatggaaac gggaacggaa
      361 ccggcctgcc gcccggcgtg ctcacgctcc cggtacctcc gccccggccg ccaaaaccga
      421 agatcaagaa gctccgggtc ctcttcatcg tgctcggtct cggcctgctc ggtttctgct
      481 cggccttctt cggcatggtc atggccgttt cgcaggattt gccggcgatc gagaactacg
      541 cgcagttccg gaccgccaag aactcgattc tcgtcgacag ccagggcaag tacatcggca
      601 ccctgtcgaa caatgagaac cagatgctgc tcgacgcgga gcagatctcg ccgaacatga
      661 agaacgcggt ggtctcgatc gaggacgcgc gcttttacga acaccgcgga atcgactttc
      721 agggtctcgg acgggcgctc gtacaggaca tcatcgcccg cagcgcgaag cagggcgcct
      781 cgaccatcac ccagcagctc gtaaagcagg cactcgaagc ccagaatgac cgctcgccgc
      841 tccagaaggt gcgcgagatg tcaatcgcct accacatcga acagcagtgg accaaggaca
      901 agatcctcac cgagtacctg aacacggtct acttcggcaa cggtgcctac ggaatcgagg
      961 cggccgcccg gacctacttc ggttacgccc acccctcctg cggcacggac gaagagccct
     1021 gcgcctcgct gctcaccccg gctgaatcgg cgctgctcgc cggcatcatc cagaaccctt
     1081 acggctacga ccccaagaac gaccccgatg cggcgctcta ccggcgcaac accgtgctcg
     1141 accgcatgct ggatcagggg tacatcaccc aggagcagca cgacgaagcg gtcaaggagg
     1201 ccctgccggc cgacgatgac attgacccgc cgagcctcga ttcgaaggcg ccctacttca
     1261 cctcctggat gcgccagcag ctggtcgacc gctacggcgc cgggcgtgcg ttcttcggtg
     1321 gcctcaaggt caagacgacg atcgacactt ggggaggcac gacaactctt ccggat
//
LOCUS       DAOQMF010000618         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_79994_length_1376_cov_3.514762, whole genome shotgun sequence.
ACCESSION   DAOQMF010000618 DAOQMF010000000
VERSION     DAOQMF010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79994_length_1376_cov_3.514762"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..631
                     /gene="dcd"
                     /locus_tag="PLV77_09570"
     CDS             65..631
                     /gene="dcd"
                     /locus_tag="PLV77_09570"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNC16139.1"
                     /translation="MVLSDKTLREEIEAGRIVIDPFEEKLIQPSSIDLRVDHSYRVFN
                     NSRYPFIDVKQPMEDLTELVKPSDDEPFILHPGEFVLGQTLERVTVPDDMVARLEGKS
                     SLGRLGLLIHSTAGFVDAGFSGNLTLELSNVANLPITIYKGMPIGQLSFMRMDRAVDT
                     AYGADGKGSKYQGQAEPTASRYYLNFRD"
     gene            693..1019
                     /locus_tag="PLV77_09575"
     CDS             693..1019
                     /locus_tag="PLV77_09575"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNC16140.1"
                     /translation="MIPNIGPLEIVIVLIIALIVFGPKRLPELGKSLGRGLNEFREGM
                     SNIGNHDDDDEDEDDAEDHEPAELTPPPSDEKLPLPGEEPEVTEVTGSSDAEPVEGEV
                     VPDSRD"
     gene            1027..>1376
                     /locus_tag="PLV77_09580"
     CDS             1027..>1376
                     /locus_tag="PLV77_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNC16141.1"
                     /translation="MARLKPVKHDEHLSLVDHLDELRTRIIYSLGFLAVAFAVCFWQS
                     DVILDVAAAPLPEAHKALVVLAPTEAFMTTVTVCAYAAIIVSAPFISYQVFAYILPAF
                     SPRERRAILPMLFT"
BASE COUNT          256 a          455 c          416 g          249 t
ORIGIN      
        1 gttgatcgcc gatggagtcg tccagagata cagctgaccg gctgctctgg gatactgccg
       61 cttcatggtc ctgagtgaca agaccctgcg ggaggagatc gaggccggac ggatcgtgat
      121 cgatcccttc gaggagaagc tgatccagcc ctcgagcatc gacctccgcg ttgaccacag
      181 ctaccgggtc ttcaacaact cgcgctaccc gttcatcgac gtgaagcagc cgatggagga
      241 tctgaccgag ctggtcaagc ccagcgatga cgagcccttc atcctccacc cgggcgagtt
      301 cgtcctcggc cagaccctgg agcgggtcac ggtcccggat gacatggtcg cccgcctgga
      361 aggcaagagc tcgctcggcc ggctcggtct cctgatccat tcgaccgcgg gtttcgtcga
      421 cgcgggattc tccggcaacc tgactctcga gctctccaac gtggccaacc tgccgatcac
      481 gatttacaag ggcatgccga tcggccagct ctccttcatg cggatggacc gggcggttga
      541 cacggcttac ggagccgacg gcaagggttc gaagtaccag ggccaggccg aacccaccgc
      601 ctcgcgctac tacctgaatt tccgggactg aactccttca ccgcccggtg atccgggccc
      661 gccggcggtt cgtaggtacg attggtgtcc agatgattcc gaatattggc ccgctcgaaa
      721 tagtcatcgt cctgatcatc gcgctgatcg ttttcggccc gaagcgcctg cccgaactcg
      781 gcaagtcgct gggccggggg ctgaacgagt tccgcgaagg catgagcaac atcggaaatc
      841 acgacgatga cgacgaagat gaagatgacg cggaagatca cgagccggcc gaactgacgc
      901 cacccccgag tgacgagaag ctcccgctgc ccggggaaga gcccgaggtc accgaagtga
      961 ccggcagctc cgacgccgag ccggtcgagg gtgaagtggt tcccgactca cgggactgag
     1021 gccgctgtgg ccaggctcaa accggtcaag cacgacgagc atctgagtct cgtcgaccat
     1081 ctcgatgaac tgcgtacgcg gatcatctac tcgctgggtt tcctagcggt tgccttcgcg
     1141 gtctgcttct ggcaaagcga cgtgatcctc gacgtcgcgg ccgcccccct gccggaggcg
     1201 cacaaggccc tggtcgtgct ggccccgacc gaagccttca tgaccacggt gaccgtctgc
     1261 gcctacgcgg cgatcatcgt ctccgccccc ttcatcagct atcaggtctt cgcctacatc
     1321 ctgccggcct tctcgccgcg cgagcgcagg gcgatcctgc cgatgctgtt cacgat
//
LOCUS       DAOQMF010000619         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_79999_length_1376_cov_3.202120, whole genome shotgun sequence.
ACCESSION   DAOQMF010000619 DAOQMF010000000
VERSION     DAOQMF010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79999_length_1376_cov_3.202120"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLV77_09585"
     CDS             <1..911
                     /locus_tag="PLV77_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16142.1"
                     /translation="EIEREIIAMRHRAGAEMVEAGDQHPVHPEPADYDFRLEDKVPTV
                     TPAELTPEILRAAILKYGCLKVTGVMDEATAKSLADDIDHAFEIRQRLSDAKEVDPTR
                     IYEEFAPNEPYQVQYVERHWVALVGGVLAADSPKMFFEMVDSLEQAGVRDVVEGYLGE
                     RAAISAQKCTLRKADPSVKGGWHQDGKFLGDVRALNVWLSLSRCGDVAPSMDIVPVRI
                     DEFVAAGVNEGDLHYQVTDETAAEAAGETGIVRPIFNPGDVLLFDEMFLHQTGSDPSM
                     PNPRYAIESWFFGPSAFPEEYAPIAF"
     gene            971..>1376
                     /locus_tag="PLV77_09590"
     CDS             971..>1376
                     /locus_tag="PLV77_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16143.1"
                     /translation="MVEPEFDSEIELGLEVKIPGQLPAGRADALFLYGYAFHRRQDVE
                     GLEILVDGQPNRAVGRMPRLDLFKSLHPLIPEGEVSETHDAGSLEDPELKSFRSGFWS
                     TVEIPATGPGKVRIEVALKLADGSISRAPLAEI"
BASE COUNT          238 a          469 c          441 g          228 t
ORIGIN      
        1 tcgagatcga gcgggagatc atcgccatgc gccaccgggc cggcgcggaa atggtcgaag
       61 ccggggatca gcacccggtc cacccggagc ccgccgacta cgattttcgc ctcgaagaca
      121 aggttccgac cgtcaccccg gccgagctga ccccggagat tctccgcgcg gcaatcctca
      181 agtacggctg cctgaaggta accggcgtga tggacgaagc gaccgcgaag tccctggccg
      241 atgacatcga tcacgccttc gagatccgcc agcgcctgag cgacgccaag gaagtcgatc
      301 cgacccggat ctacgaggag ttcgccccaa acgagcccta ccaggtccag tacgtggagc
      361 ggcactgggt cgccctggtc ggcggggtcc tcgcggccga ctcgccgaag atgttcttcg
      421 agatggtcga ctcgctcgag caggccgggg ttcgcgacgt ggtcgagggt tacctcggcg
      481 agcgggccgc catttccgcc cagaaatgca ccctgcgcaa ggccgatccg agcgtgaagg
      541 gcggctggca tcaggacggc aagttcctcg gtgacgtccg tgccctgaac gtgtggcttt
      601 cgctttcgcg ctgcggtgat gtcgccccga gcatggacat cgtcccggtc cggatcgacg
      661 agttcgtcgc agccggcgtc aacgaaggtg acctccacta ccaggtgacc gacgagaccg
      721 cggccgaagc ggcgggtgaa accggcatcg tccggccgat cttcaacccc ggtgacgtgc
      781 tcctcttcga cgagatgttc ctccaccaga ccggctcgga tccttcgatg ccgaacccgc
      841 gttacgcgat cgagagctgg ttcttcggac cctcggcctt ccccgaggag tacgcgccga
      901 tcgcattttg agccgggcgg gaggccgttc ccggccacat ccggcccgag ttcccaatag
      961 tctcgtctga atggtcgagc ctgaattcga ttccgagatc gagctcgggc tggaggtaaa
     1021 gatccccggg cagctgccgg ccggccgggc cgacgcgctc ttcctttacg ggtacgcatt
     1081 tcaccgccgc caggacgtcg agggcctgga gatcctggtc gacggccagc cgaaccgggc
     1141 ggtcgggcgc atgccccgcc ttgacctctt caagtcgctt cacccgctga tcccggaggg
     1201 cgaagtctcc gagacccatg acgccggctc gctggaggac cctgaactga agagcttccg
     1261 ctccggtttc tggtccacgg tcgagatccc cgccaccgga ccgggcaagg tcaggatcga
     1321 agtcgccctg aagcttgcgg atggcagcat cagccgcgcc cccctcgccg agatcg
//
LOCUS       DAOQMF010000620         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_80184_length_1374_cov_5.735406, whole genome shotgun sequence.
ACCESSION   DAOQMF010000620 DAOQMF010000000
VERSION     DAOQMF010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80184_length_1374_cov_5.735406"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..>1374)
                     /gene="hemA"
                     /locus_tag="PLV77_09595"
     CDS             complement(167..>1374)
                     /gene="hemA"
                     /locus_tag="PLV77_09595"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNC16144.1"
                     /translation="SEATVISTCNRTEIYVFAHDPVVAESSILSALASQGGISPTELA
                     GHLYSLRGDDAALHLFRVTAGLDSMIIGESEVQGQVKRAHELALVEGVSGPILNRLFR
                     GALAAGGRARNETGISRTGVSMASVAVEQATRAIGEVGDRRALIIGAGETSELVAGAL
                     RARGAESVFVANRHLEKACDLARKFGGTAAPLTELSEQVAEADLVVSATMSPHHLIDR
                     ELLEPVMKERQERPVFLVDLAVPRDIDPRVRELPGVSLTDVDELQVAVARNLGRREAE
                     ARRAEHVVQAELELFQRWLQSLEVMPVIVGLRDKADEIVARVLSENEGRWEAMSEEDR
                     RRVETLARTIVGRMLHEPTLRLKEIAGSDESFEVLATVRGLFGLDSGTDPGDGEVATV
                     TPIRREGNS"
BASE COUNT          205 a          477 c          475 g          217 t
ORIGIN      
        1 tgaccatcga gcagcagcct ttcgaccccg cgggtgaagc gctccttgtc cccggcctcg
       61 ggcggaaggt cggcgtcgac ctcgaccagt tccgcctgtg gtccaagcgc ctcggccacc
      121 tggccggcct gggccagggc gagggcactg gaacgggttg cgaccctcag gagttgcctt
      181 cccggcggat cggggtgacc gtggcgactt cgccgtcgcc ggggtcggtc ccgctgtcga
      241 ggccgaagag gccacggacc gtggcgagca cctcgaagga ctcgtcgctg ccggcgatct
      301 ccttcaggcg cagggtcggc tcatgaagca tgcgaccgac gatcgtccgg gccagggtct
      361 cgacccggcg ccggtcctct tcgctcatcg cctcccagcg gccttcgttc tcgctgagaa
      421 cacgggcaac gatctcgtcg gccttgtcac gcaggccgac gatcaccggc atcacctcaa
      481 gcgactggag ccagcgctgg aagagctcaa gctcggcctg gacgacatgc tcggcccggc
      541 gggcttcagc ttcgcgccgg ccgaggttgc gggccaccgc gacctgcagt tcgtcaacgt
      601 cggtcaggct caccccgggg agttcccgca cccgcgggtc aatgtcccgc gggaccgcca
      661 ggtcgacgag gaagaccgga cgctcctggc gttccttcat gaccggttcg agcagttcgc
      721 gatcgatcag gtggtggggt gacatggtcg ccgaaacgac caggtcggcc tcggccacct
      781 gctcgctcaa ctcggtcagc ggtgcggccg taccaccgaa cttccgggcc aggtcgcagg
      841 ccttttcaag gtgccggttg gcgacgaaga cgctttcggc cccgcgggca cgcagggccc
      901 cggccacaag ttccgaggtc tcgccggcgc cgatgatcag ggcccggcga tcaccgacct
      961 ccccgatcgc ccgggtcgcc tgttcaaccg cgaccgaggc catcgagact ccggtccggc
     1021 tgattccggt ctcgttcctg gcccggcccc cggcggcgag ggcaccgcgg aagagccggt
     1081 tgaggatcgg gccggagacg ccctcgacca gcgccagttc atgagcccgc ttgacctgac
     1141 cctggacctc ggactcgccg atgatcatcg agtcgagccc ggcggttacc cggaagaggt
     1201 ggagcgccgc gtcgtcgccg cgcagcgagt agaggtggcc ggcaagttcg gtcgggctga
     1261 ttccgccctg gctggcaagg gccgagagga tgctggactc ggcgaccacc gggtcgtggg
     1321 cgaagacata gatctcggtc cggttgcagg tcgagatgac cgtcgcttcc gagg
//
LOCUS       DAOQMF010000621         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_80198_length_1374_cov_4.279757, whole genome shotgun sequence.
ACCESSION   DAOQMF010000621 DAOQMF010000000
VERSION     DAOQMF010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80198_length_1374_cov_4.279757"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1055
                     /locus_tag="PLV77_09600"
     CDS             84..1055
                     /locus_tag="PLV77_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16145.1"
                     /translation="MKTVQTVTGEIAADELGRTLIHEHFYSSDEAVSVQWPHVRDREA
                     EHGLAVESAEAVKAHGVRTVVEPTAMLLGRDIQASRELAEETGLQIIVCTGIYTYDHL
                     PQFLLNRDADYIASLFVHDIEEGIQNTGVKAAFIKCAADEPGLTPNIEKIHRAAARAS
                     LQTGAPIMAHSRPASGTGPKQVEVLLEEGVPAERIQIAHTGDTDDLGYIEGLLDKGVY
                     IGMDRYGLDLFLPTAQRNATVIELLNRGYADRMFLSQDFDIPIANGLDWYPLEVLEQL
                     QAAGAATDWSMTFIFEQVIPALREAGMTGDQLDTMLVENPKRWLGRS"
     gene            complement(1069..>1374)
                     /locus_tag="PLV77_09605"
     CDS             complement(1069..>1374)
                     /locus_tag="PLV77_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16146.1"
                     /translation="GWLYTMSTLNLVGTTISLTGGLYTSEDGFAEPIQQPATLCTASP
                     ALTGVVPPGQISTFACDNIGLDVSAGEVGFFAVEANATGVSLNNSLSLRGSMSMVVD"
BASE COUNT          290 a          454 c          418 g          212 t
ORIGIN      
        1 gctgctgatc gacccaccca gcgaccgccc ctgaccgggg cgggagaacc cacaggcaac
       61 cgccgaaagc gaagaggaga ggcatgaaga ccgttcagac cgtcaccggt gagatcgcag
      121 ccgacgagct cggccggacg ctcatccacg agcacttcta ctcatcggac gaggcggttt
      181 ccgtccagtg gccacatgtt cgcgaccggg aagccgaaca cgggctggcc gttgaatcag
      241 ccgaggcggt caaggcccac ggcgtgagaa cggtggtcga gccgaccgcg atgctgcttg
      301 gtcgcgacat ccaggcgagc agggaactcg ccgaggaaac cggtctccag atcatcgtct
      361 gtaccgggat ctacacctac gaccatctcc cccagttcct gctcaaccgc gatgccgatt
      421 acatcgcctc actctttgtc catgacatcg aggaaggtat ccagaacacc ggagtcaagg
      481 ccgctttcat caagtgcgcc gccgacgaac ccggcctcac ccccaacatc gagaagatcc
      541 accgggcggc agcccgggca agcctccaga ccggcgcccc gatcatggcc cacagccgcc
      601 ccgcttccgg gaccggtccg aagcaggtcg aggtccttct ggaggaagga gtgccggccg
      661 agcgaatcca gattgcccac accggtgaca ccgacgacct gggctacatc gagggcctgc
      721 tggacaaggg cgtttacatc ggcatggacc gctacgggct ggacctcttc ctccccaccg
      781 ctcaacgcaa cgcgacggtg atcgaactgc tgaaccgcgg ctacgcggac cgcatgtttc
      841 tctcccagga tttcgacatc ccgatcgcca acggcctcga ctggtacccg ctcgaggtgc
      901 tcgaacagct tcaggcggcc ggcgcagcga ccgactggag catgaccttc atcttcgagc
      961 aggtaatccc ggcgctcagg gaagccggaa tgactggcga tcagctcgac acgatgctgg
     1021 tcgagaatcc gaagcggtgg ctgggtcgta gctgaggctg accgcgggtc agtccacgac
     1081 catcgacatc gatccccgga gggaaagaga attgttcagc gacaccccgg tggcgtttgc
     1141 ctcgaccgca aagaaaccga cttcgccggc agagacatcg agaccgatgt tgtcgcacgc
     1201 gaaggtggaa atctgaccgg gcggcactac tcccgtaagg gcaggtgaag ccgtgcacaa
     1261 ggtcgccggc tgctgaatcg gctcggcgaa cccgtcttcg ctggtgtaga gccccccggt
     1321 aagtgaaatg gtcgtgccca ccaggttcag ggtcgacatc gtgtagagcc aacc
//
LOCUS       DAOQMF010000622         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_80422_length_1372_cov_3.720577, whole genome shotgun sequence.
ACCESSION   DAOQMF010000622 DAOQMF010000000
VERSION     DAOQMF010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80422_length_1372_cov_3.720577"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1238)
                     /gene="dnaN"
                     /locus_tag="PLV77_09610"
     CDS             complement(126..1238)
                     /gene="dnaN"
                     /locus_tag="PLV77_09610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNC16147.1"
                     /translation="MKFSIKNNELSGKLALVARTLATGGVNPALGGILVEATDGRLSF
                     SSTDSNLSLRVPVHVEGSVESDGTVLLPGRLFADISRLLGSGQSEIEYLASERKVSVK
                     SGSSSFNLRTLNEKDFPPLPDASGETISLGRESLLETIELVARAASRDDMRPVLTSVQ
                     VLASGNELTMVATDSYRLAVKQTHLAEAVSVELDRNIPANALRELSRLLSASESDSVR
                     LSVTDRLVVFNVDGSILSTNTVDGQFPKYQQLFPETWDHDVRLPRTELLESVRRVSQM
                     AQKNRPLKIALTPGELTISAETPDLGDAEEKMPANFDGEELAIGFNHEFFRDGLEAIP
                     GDEVLLRLISPLRPGLLQPVDSEDFRYLVMPIRLNE"
BASE COUNT          274 a          385 c          464 g          249 t
ORIGIN      
        1 acggcaggcc ggcgagactc aacccgatca gagcgatgat cacggtcagg ccggggtcgg
       61 gttcgaaaat caccccggcc agagatcgga cctggtccgc ctcgacccgg gaaacgatca
      121 cgtgcctact cgttgagacg gatcggcatg accaggtagc ggaagtcttc actgtcgacc
      181 ggctgcagca gaccgggccg caggggagaa atcagtcgca gcaggacttc gtcaccggga
      241 atcgcctcga gaccgtcgcg gaagaactcg tggttgaaac cgatcgccag ttcttcgccg
      301 tcgaagttcg ccggcatctt ctcctcggcg tcgcccaggt ccggagtctc ggccgagatc
      361 gtcagctcgc cgggggtaag ggcgatcttc aagggccggt tcttctgggc catctggctg
      421 acccggcgaa ccgattcaag gagttccgtc cgcggcagcc gtacgtcgtg atcccaggtt
      481 tccgggaaga gctgctggta cttggggaac tggccgtcaa ccgtgttggt cgaaaggatc
      541 gagccgtcga cgttgaagac gacgagccgg tcggtcaccg agaggcgaac cgaatccgat
      601 tcggaagccg aaagcaggcg cgagagttca cgcagtgcgt tggcgggaat gttccggtca
      661 agttcgaccg aaactgcttc cgccaggtga gtctgcttta cggcgagccg gtaggagtcg
      721 gtggccacca tggtcagctc gtttccggaa gcgagcacct ggacactggt cagcaccggg
      781 cgcatgtcat cgcgggaggc ggcacgggca accagctcga tcgtttcgag cagtgactcc
      841 cggccgagcg agatcgtttc tccgctggcg tcgggaaggg gcgggaaatc cttctcgttg
      901 agggtgcgaa ggttgaagct cgaggatccc gacttgacgc tcaccttccg ctcggaagcc
      961 aggtactcga tctcggactg tccggacccg agcagacggg agatgtcggc aaagagccgg
     1021 ccgggaagga ggacggtgcc gtcggactca accgacccct cgacatggac gggaacccgc
     1081 aggctcaggt tgctgtcggt tgaactgaag gagaggcgcc cgtcggtcgc ctcgaccagg
     1141 attccgccaa gagccggatt gactccaccg gtcgcgagcg tcctggcgac cagggcgagt
     1201 ttgcctgaaa gctcgttgtt cttgatcgag aacttcaaat ccgattacct cgtttaagtt
     1261 tggtagttct gtggagtaaa gcgcagaacc ggtggaagtg gcttggggaa gccaaaatca
     1321 ctgtggggag aaatgtggaa aaagggattc tttcagcgcg ttcggcggct gg
//
LOCUS       DAOQMF010000623         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_81792_length_1360_cov_4.203065, whole genome shotgun sequence.
ACCESSION   DAOQMF010000623 DAOQMF010000000
VERSION     DAOQMF010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81792_length_1360_cov_4.203065"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PLV77_09615"
     CDS             <1..1248
                     /locus_tag="PLV77_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNC16148.1"
                     /translation="HATANGDPRPGPVHLNFPLRDPLAPVPEDEAVTATRRLALEGRP
                     GGPLTTVEKASCGADRSTVNRLVSMVQKAERILILAGRQTDPSIREPLARLANACRAP
                     VLAEPTSQFRLGPHDRGAVVTAYERIARRREKGLAPDLIIRLGEFPTSKQLRLWTAET
                     DPAEIVVDPGYGWYEPVRTADLIVRCDPSTLLHQIENTAEQRVGGPAGSGFWNKWMEA
                     ERKERAEIEAAWDPAGGSEGPLEAGAVHARLAELQTDGNLVYTASSMAIRDQEESLPA
                     LDRDVFFLANRGANGIDGLIASGIGAAKATGRPVTVITGDLGFFHDVGSLALARGLEV
                     PVRIVVLNNDGGGIFERLPQKQAMTGGEFEQLMRTPSGLSVEDAARLYGLPFHLAPRL
                     DALEAAFTADAESVSVIEVPVTG"
BASE COUNT          220 a          478 c          456 g          206 t
ORIGIN      
        1 cacgccacgg cgaacggcga cccccggccc ggaccggttc atctgaactt ccccctgcgc
       61 gacccgctgg ctccggttcc ggaagatgag gcggtcaccg cgacccggcg cctcgccctc
      121 gaaggtcggc cgggcggacc gctcaccacg gtcgaaaagg cttcctgcgg cgccgaccgg
      181 tcgactgtca accggctcgt ttcgatggtc cagaaagcgg aacggatcct gatcctcgcc
      241 ggacgccaga ccgatccatc gatccgggaa ccgctcgcac ggctcgccaa tgcctgtcgc
      301 gcccccgtgc tggccgagcc gacctcccag ttccggctcg gtccccatga ccgcggggcc
      361 gtggtcaccg cctacgaacg aatcgcccgc cggcgcgaga aggggctcgc gcccgacctg
      421 atcatcaggc tcggcgaatt cccgacctcg aaacagctgc gcctctggac ggccgagacc
      481 gatccggccg agattgtggt cgaccccggc tacggctggt acgagccggt tcgcacagcc
      541 gacctgatcg tgcgctgcga cccctccacc ctcctccacc agatcgagaa caccgcggag
      601 cagcgggtcg gaggcccggc cggcagtggc ttctggaaca agtggatgga ggccgagcga
      661 aaggaacgcg ccgagatcga agcggcatgg gatccggccg ggggaagcga aggacccctg
      721 gaggccggcg cggtgcacgc ccggctggct gaacttcaga ccgacgggaa cctcgtctac
      781 accgcctcga gcatggcgat tcgggatcag gaagaatcgc tgcccgccct cgatcgcgac
      841 gtcttcttcc tcgccaaccg cggtgccaac ggcatcgatg gtttgatcgc ctcgggaatc
      901 ggggctgcga aggccaccgg caggccggtc acggtcatca ccggtgatct cggcttcttc
      961 catgacgtcg gcagcctcgc cctggcccgc ggcctcgagg tcccggtccg gatcgtggtc
     1021 ctgaacaacg atggcggcgg aatcttcgag cgcctgccgc agaagcaggc aatgaccggg
     1081 ggcgagttcg agcagttgat gcggacgcct tccggcctga gcgtcgagga tgcggcgcgg
     1141 ctctacggcc tgcccttcca cctcgctccc cggcttgacg cgctcgaggc cgccttcacg
     1201 gctgacgccg aatcggtctc ggtgatcgaa gtcccggtca ccggctgaat ccgcggcgag
     1261 tgcccggttt gtgccggtat gtggcgcaaa gcgggttctc gacgagaggg tggctagctg
     1321 agcaggggca gcagggcgcc gagcttgatc tcggtctcgg
//
LOCUS       DAOQMF010000624         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_81917_length_1359_cov_3.337423, whole genome shotgun sequence.
ACCESSION   DAOQMF010000624 DAOQMF010000000
VERSION     DAOQMF010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81917_length_1359_cov_3.337423"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLV77_09620"
     CDS             <1..665
                     /locus_tag="PLV77_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNC16149.1"
                     /translation="SHDRESGRLSLEFPDGATVEGEVGLGEPEEVTFFGQPERARPVT
                     GDFSDAVSRHTGARLRLMAHPEGRPGVDRGKWGGVTLLGAASLESLREAGRDFNCAEP
                     SSEALPGGTASDPDPGPIDGRRFRMTFGVEGTRPYEEDDWTGREIRIGEALVRVAAHA
                     GRCAVTTRDPDTGVTDLKTLHFLKHSRDGVDSFEPLPFGVYGEVLEPGPVRIGDPVGL
                     AG"
     gene            690..1091
                     /locus_tag="PLV77_09625"
     CDS             690..1091
                     /locus_tag="PLV77_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNC16150.1"
                     /translation="MLFGQQHVDRYQATGGEEGHDWQGTHTLLLTTTGRKSGEKRTTP
                     LIYDPVGDAYAIVASKGGADEPPAWYLNLQADPTVEVQVKADKFTARARTASEEEKAG
                     IWPQMVKQWPAYDDYQAATDRPIPIVLLERA"
     gene            complement(1095..>1359)
                     /locus_tag="PLV77_09630"
     CDS             complement(1095..>1359)
                     /locus_tag="PLV77_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16151.1"
                     /translation="MIPSRQRATILSFDSMLGSTGGGVVQPALGRVADTSGYATSYLY
                     GAMITVFALPFILLSRHQHPPADRATGTPEAEGTVEAGPESDR"
BASE COUNT          230 a          421 c          507 g          201 t
ORIGIN      
        1 cctcacacga ccgggaatcg ggcaggctga gccttgagtt tcccgacggc gcgaccgttg
       61 agggcgaggt cgggctcggt gagcccgagg aggtcacttt cttcggccag cccgagcggg
      121 cccggccggt caccggtgat ttctccgacg cggtctcgag gcacactggg gccaggctgc
      181 ggctgatggc ccacccggaa gggcggcccg gggtggatcg cggcaaatgg ggcggcgtga
      241 ccctgctcgg tgcggcttcg ctggagagcc tgcgcgaggc ggggcgggat ttcaactgcg
      301 cggagccttc aagcgaagcg ctgccgggcg gaactgcgag cgatcccgac ccggggccga
      361 tcgacgggcg gcgcttccgc atgacctttg gcgtcgaagg aaccaggcct tatgaggagg
      421 acgactggac cggccgggaa attcggatcg gcgaagccct ggtcagggtc gcggcccacg
      481 ccggccgttg cgcggtaacc acccgcgacc ccgacaccgg ggtgaccgac ttgaagaccc
      541 tgcatttcct caagcactcg cgtgacgggg tcgattcgtt tgagccgctg cccttcggcg
      601 tctatggcga ggttctggaa ccggggccgg tgcggatcgg tgacccggtc ggtcttgccg
      661 ggtgaggggt ccgcggttag gttgggatca tgttgttcgg acagcagcac gttgatcgtt
      721 atcaggccac cggcggtgaa gagggtcatg actggcaggg cactcacacc ctgctgctca
      781 ccaccacggg gcgaaaatcc ggtgagaagc ggaccacgcc gctgatctac gacccggtcg
      841 gggatgccta cgcgatcgtc gcctcgaagg gcggggcgga cgaaccgccg gcctggtacc
      901 tgaacctgca ggccgacccg acggtcgagg tccaggtgaa ggcggacaag ttcacggccc
      961 gggcgaggac cgcgagcgag gaagaaaagg ccgggatctg gccgcagatg gtcaaacagt
     1021 ggccggccta cgacgattac caggcggcaa ccgaccggcc gatcccgatc gtcctgctcg
     1081 aaagggcctg aacctcaccg atcagattcg ggcccggcct cgaccgtgcc ttccgcctcc
     1141 ggagttccgg tcgcgcggtc ggccggcggg tgctggtgcc gggacaggag gatgaacgga
     1201 agggcaaaga cggtgatcat cgcgccgtag aggtaggagg tcgcgtaccc ggaggtgtcg
     1261 gcgacccggc cgagcgcggg ttggaccacg cccccgccgg tcgatcccag catcgagtcg
     1321 aaggaaagga tcgtcgcccg ctggcgggag gggatcatc
//
LOCUS       DAOQMF010000625         1357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_82228_length_1357_cov_1.562212, whole genome shotgun sequence.
ACCESSION   DAOQMF010000625 DAOQMF010000000
VERSION     DAOQMF010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82228_length_1357_cov_1.562212"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1357)
                     /gene="murJ"
                     /locus_tag="PLV77_09635"
     CDS             complement(<1..>1357)
                     /gene="murJ"
                     /locus_tag="PLV77_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNC16152.1"
                     /translation="DPQLDPFEAAARAAEEAIRNTSEKATRVTRQPVRNPDPVPEPVR
                     EREDALVETASGLAEEGGAIAPVPVIEGTELDPEALAPTPAPEVKAPAPEPDGALKPE
                     KPGRLARSTAFFSIATAASRVAGLVREIVAAGHFGISGPMSAFTVAFQVPNLIRALFA
                     DAALQPGFVPIFTEQIEKGKYREAFRMASTLLLLVTMILGAITAFFILAAGLVMPIFA
                     PGFSAATEDLMIVLSQILFPILILLGVTGIVVGILNSYDRFGAFAISPFFWNITIIAV
                     VIIGVPLFPEDKQIYAYAIGVVAGTLVQLLIPTFDLRNTPFQFTLKVDFRDPNVRRVL
                     IMMIPVTLSLGLINFNLLINSFFGSLVSDEAPAAIDKAFRIYQLPQGIFSVAIATVLF
                     PTLARFANRKDFDSLRSTLAKGMRQIIFVLLPASAAILVLSEPVIRLVYQRGEFNAAE
                     TE"
BASE COUNT          250 a          401 c          484 g          222 t
ORIGIN      
        1 cctcggtctc ggctgcgttg aactcgcctc gctggtagac gaggcggatc accggttcgg
       61 agagcacgag gatcgcggcg ctggccggca ggagcacgaa gatgatctgc cgcatgccct
      121 tggccagtgt gctccgcagc gagtcgaagt ccttgcggtt ggcaaaacgg gccagggtcg
      181 ggaagaggac cgtcgcaatc gccaccgaga agatgccctg cgggagctga taaatccgga
      241 aggccttgtc gattgccgcc ggggcctcgt cactgaccag cgagccgaag aagctgttga
      301 tcagcaggtt gaagttgatc aggccgaggc tgagcgtcac cgggatcatc atgatcagca
      361 cccgtcttac gttcggatcc ctgaaatcga ccttgagggt gaactggaag ggcgtgttcc
      421 ggaggtcgaa ggtggggatc aggagctgga ccagggtgcc cgccacgaca ccgatcgcgt
      481 acgcgtagat ctgcttgtcc tcggggaaga gcggcacccc gatgatgacc accgcgatga
      541 tcgtgatgtt ccagaagaac ggcgatatcg cgaaggcgcc gaagcggtcg tagctgttga
      601 ggatgccgac cacgatcccg gtcaccccga gcaggatcag gatcgggaac aggatctgcg
      661 aaagcacgat catcaggtct tcggtggcgg ccgagaagcc gggagcgaag atcggcatga
      721 ccaggccggc ggcgaggatg aagaaggcgg tgatcgcgcc gaggatcatg gtcaccagca
      781 ggagcagggt cgaggccatg cggaaggcct cccggtactt gcccttctcg atctgctcgg
      841 tgaagatcgg gacgaagccg ggctgcagcg cggcatcggc gaaaagtgcc cggatcagat
      901 tcggaacctg gaaggcgacg gtgaaggccg acatcggtcc cgagatgccg aaatgccctg
      961 cggcgacgat ttcgcgaacc agcccggcca cccgggaggc ggcggtggcg atcgagaaga
     1021 aggcggtcga gcgggccagg cgtcccggct tctccggttt gagggccccg tcgggttcgg
     1081 gggcgggcgc tttcacctcc ggtgccggcg tcggagccag ggcctcgggg tcgagttccg
     1141 ttccctcgat caccgggacc ggggcaatgg ctcccccctc ctcggccagc ccggatgcgg
     1201 tttcaaccag cgcgtcttcc ctttcccgca ccggttcagg gacgggatcc ggattcctca
     1261 ccggctgccg cgtcacccgg gtggccttct ccgaggtgtt gcggatcgcc tcttcagccg
     1321 cacgggcggc agcttcaaag gggtcgagtt gggggtc
//
LOCUS       DAOQMF010000626         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_82564_length_1354_cov_2.531178, whole genome shotgun sequence.
ACCESSION   DAOQMF010000626 DAOQMF010000000
VERSION     DAOQMF010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82564_length_1354_cov_2.531178"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLV77_09640"
     CDS             complement(<1..487)
                     /locus_tag="PLV77_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNC16153.1"
                     /translation="MYPVTYEADYLRERNRLTTFFRLIVAIPWLIVAYIYVIAQMVVV
                     FIAWFAILFVGRFPEGMYNFVGGILRFTQRVNGFMYLQTDAWPPFGIGDDDSYPIRVK
                     FHPPAARQSRMKDLFRLILAIPLLVLSYAMSYALMAVALISWLTIVFRGYQPAAIHNA
                     IA"
     gene            630..>1354
                     /locus_tag="PLV77_09645"
     CDS             630..>1354
                     /locus_tag="PLV77_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC16154.1"
                     /translation="MTDFAKIPDDLKPADGRFGCGPSKVRPEALESLAERSDLMGTSH
                     RQKPVKDLVGRVRGGLGELFSIPDGYEIALGNGGATAFWDAAAAWLVRDRALHLVYGE
                     FSRKFSQVTAGAPFLSDPVLIEAEPGSAPAPESRDGVDVIGWAQNETSTGVMVPVERP
                     AGADDALVLIDATSGAGGLPVALEETDVYYFAPQKAFGSDGGLWLAALSPAAIARIEE
                     LDGTSGRWQPPFLSLKTALDNSR"
BASE COUNT          242 a          429 c          446 g          237 t
ORIGIN      
        1 aggcaatcgc gttgtggatc gccgccggct ggtaaccgcg gaaaacgatg gtcaaccagg
       61 agatcagcgc gacggccatc agggcgtagc tcatggcgta ggagagaacg agcagcggga
      121 tcgccaggat gagccggaac aggtccttca tgcggctttg ccttgcggcg gggggatgga
      181 acttgacccg gatcgggtac gagtcgtcgt cgccgatgcc gaagggcggc caggcgtcgg
      241 tctgcaggta catgaagccg ttgacccgtt gcgtgaagcg gagaatcccg cccacgaagt
      301 tgtacatccc ctccgggaac cgcccgacga agaggatcgc gaaccaggcg atgaaaacca
      361 ccaccatctg ggcgatcacg tagatgtagg ccacgatcag ccacggaatc gcgacgatca
      421 ggcggaagaa ggtggtgagc cggttccgtt ctcgcagata gtcggcttca tatgtgacgg
      481 gatacaagat tctctccttg ggttcggggg gactggtcga actttcattc agactatccg
      541 tcctcccgga cctcgtgaag atgcatttgc gacaggccgt ccaggggcgg gaatctgtcg
      601 agctcggcga ctcaatatcc tcacccgaga tgactgattt cgccaagatc ccggatgacc
      661 tgaagcccgc cgacggccgc ttcggctgtg gcccctcgaa ggtgcgaccc gaagcccttg
      721 agtcgctggc cgagcgatcg gacctgatgg gtacctccca tcggcagaag ccggtcaagg
      781 atctggtcgg acgcgtccgc ggcggcctcg gagagctctt ttcgattccc gatggttatg
      841 aaatcgccct cggaaacggc ggggcaaccg cattctggga tgccgcggcg gcgtggctgg
      901 tccgggaccg ggcgctccac ctggtctatg gcgagttttc ccgcaagttc tcgcaggtca
      961 cggccggggc cccgtttttg tccgaccccg tcctgatcga agccgagccg ggcagcgccc
     1021 ccgcgcccga aagccgtgac ggcgtcgacg tgatcggctg ggcccagaac gagacctcga
     1081 ccggagtcat ggttcccgtc gagcgtcccg ccggcgccga cgacgccctg gttctgatcg
     1141 acgcgacctc cggtgccggt ggccttccgg tcgccctcga ggagaccgac gtctactact
     1201 tcgcccccca gaaggcgttc ggttccgacg gcggtctctg gctggccgcg ctgagccctg
     1261 ccgccattgc ccggatcgag gagctggacg gtacatccgg ccgctggcag ccgcccttcc
     1321 tgtcgctgaa gacggcgctc gataactccc gcaa
//
LOCUS       DAOQMF010000627         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_82865_length_1351_cov_2.655093, whole genome shotgun sequence.
ACCESSION   DAOQMF010000627 DAOQMF010000000
VERSION     DAOQMF010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82865_length_1351_cov_2.655093"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1351)
                     /locus_tag="PLV77_09650"
     CDS             complement(<1..>1351)
                     /locus_tag="PLV77_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNC16155.1"
                     /translation="LDPFVIGTCLPWNKPMNYVAKVELFEKRWQGFLLCRVGAFPIRR
                     GEADELAIETAELVLARGGTICIFPEGTRIRKGSLGKPKRGVGRLALRSGAPVLPVAV
                     TGSENVRRGWQIRPRKVDIRVGRPMSFPQSESPSPALAASVTDRIWPNVELQWEDIGG
                     LPPMRRAAVIGAGSWGTAVAVLLARGGLEVQLGTRTADQAEEMALARENTRYLPGVRL
                     PDGIEVRASSQIELGGVDLLCIAVPSASYPQAVGAVADRVGRRAGVLLLAKGLIAPKG
                     QLPSEYVGERIRSRGVSCLGGPAHAKEAVSGSAALVLGSADGDLRAQLGDVFDRAGLV
                     CERSRDVVGVEMAGAAKNAAVLAAAAAEPHGLNAAGIAAAAVWDECVGYAVKHGADLE
                     TFNGLAGVGDLLATVMASGSRNRRAGELLGKGSAPDQIRETIGQASEALDSVPQIAAT
                     "
BASE COUNT          219 a          482 c          453 g          197 t
ORIGIN      
        1 ggtggcggcg atctggggga ccgaatcgag agcttccgaa gcctggccga tcgtttcgcg
       61 gatctggtcc ggggcgctgc ccttgccgag cagttcaccc gcccggcgat tgcgactgcc
      121 ggatgccatc accgtggcaa gcaggtctcc gaccccggcg aggccgttga aggtttcaag
      181 gtccgcccca tgttttacgg cgtaaccgac gcattcatcc cagaccgcgg cggcggcgat
      241 cccggccgca ttcaggccat gtggctcggc cgcagcggcg gccagcacgg cggcattctt
      301 cgccgccccg gccatctcca ccccgaccac gtcccgcgag cgctcgcaga ccagaccggc
      361 ccggtcgaac acgtcaccga gttgagcccg aaggtcaccg tcggcgctgc caagaaccag
      421 agcggccgaa ccggacaccg cttcctttgc gtgtgcggga ccaccaaggc aggagacgcc
      481 gcgtgagcgg atccgctcac ccacgtattc ggatggcagt tgacccttgg gggctatcaa
      541 ccccttggcg agaagcagta ccccggcccg gcgaccgacc cggtcggcga ccgctccgac
      601 cgcctgcggg taggaagctg acgggaccgc gatgcagagc aggtcgactc caccgagttc
      661 gatctggctg gaggcccgga cttcgatgcc gtcgggcagg cgaacgccgg gcagatacct
      721 ggtgttctcg cgcgcgaggg ccatctcctc cgcctggtcg gcggtgcggg tgccgagctg
      781 gacttcgagc ccgccgcggg ccagcagcac cgcgaccgcg gtgccccagc tgccggctcc
      841 gatgaccgcg gcccggcgca tcggcggcag accgccgatg tcttcccatt gaagttcgac
      901 gttcggccag atgcgatcgg tgaccgatgc ggcgagggcc ggagaaggcg attccgactg
      961 gggaaaggac atcggccggc cgacccggat gtcgaccttg cgcggccgga tctgccagcc
     1021 gcgccgcacg ttctcggaac cggtgacggc gaccggcaga accggtgccc cgctccggag
     1081 agcgagacgc ccgacgcccc gcttcggctt gccgagcgaa cccttcctga tccgggtgcc
     1141 ttcgggaaag atgcagatcg tcccgccccg ggccagaacc agctcggcgg tttcgatcgc
     1201 cagctcgtca gcctctcccc ggcgaatcgg gaaggccccg acccggcaga ggaggaaccc
     1261 ctgccagcgc ttctcgaaca gttccacctt cgccacgtaa ttcatcggct tgttccaggg
     1321 caggcaggtg ccgatcacga acgggtcgag g
//
LOCUS       DAOQMF010000628         1349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_83053_length_1349_cov_3.316847, whole genome shotgun sequence.
ACCESSION   DAOQMF010000628 DAOQMF010000000
VERSION     DAOQMF010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83053_length_1349_cov_3.316847"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLV77_09655"
     CDS             <1..664
                     /locus_tag="PLV77_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16156.1"
                     /translation="ARSEGELDQTREAKLFELYERGEIESLSNEDQNRRNELRDQIRR
                     LNEELKREKEDRNRAEIERDEAKRGRDETVKENRGMRTGWLISVICLVVGMCLVVALF
                     LLLGGNNDASGSQCDDANAAGDLTLEDVGEIVNVKGQIAQISKVNEATFLNLGGPYGA
                     QDLAVVIWGGNLENWATPPDVKYDGREVSVTGKVDLYDDPRYGESLEIEANSPNDIAI
                     CS"
     gene            complement(704..777)
                     /locus_tag="PLV77_09660"
     tRNA            complement(704..777)
                     /locus_tag="PLV77_09660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(741..743),aa:Arg,seq:ccg)
     gene            816..>1349
                     /locus_tag="PLV77_09665"
     CDS             816..>1349
                     /locus_tag="PLV77_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC16157.1"
                     /translation="MDLSVAFLGTGGPVPTALRGTASVLITRGGEKLMFDCGEGTQRQ
                     MRRSMGLVQVDEIYLTHFHLDHCLGLPGLLKTYDLNDREEPLMLFGPVGLHGLMKDLQ
                     PLIGRLDYHLEVEELEGGDVVPHVDYEIECFPVEHRTRALGYALLEDDRPGRFDPAEA
                     ERLGVKAGPDFKRLQDGE"
BASE COUNT          313 a          361 c          433 g          242 t
ORIGIN      
        1 agccaggtca gaaggggaat tggatcaaac aagggaagcc aagctgttcg agctgtacga
       61 gcgcggcgaa atcgaaagtc tctctaacga agatcaaaat cgccgaaacg agctccgaga
      121 ccagattcga cggctgaatg aggaactgaa gagagagaag gaagatcgga accgtgctga
      181 gatagaacgg gacgaggcca agcgaggacg agacgaaacg gtgaaggaga atcgcggaat
      241 gagaaccggc tggctcatct cggtcatttg tctggttgta ggtatgtgcc tggtggtggc
      301 tctttttctc ttgctagggg gaaataacga cgcttcgggg tctcaatgcg atgacgcgaa
      361 cgcggcaggc gatcttaccc ttgaagacgt gggcgagata gtgaacgtga agggacaaat
      421 cgctcagata tccaaagtaa atgaagctac gttcctgaat ctcggaggac cgtatggcgc
      481 ccaagatctg gcggtggtga tctggggagg taatctcgaa aactgggcta cgccacctga
      541 tgtgaagtac gatgggcgtg aagtgtcggt tacaggaaag gttgatctct acgacgatcc
      601 cagatacgga gagtcacttg agatagaggc caattcacct aacgacatag cgatctgttc
      661 gtaaagatcc ggtcaaaaat cagaaactgg ccatatcgga agacgcgccc ggcaggattc
      721 gaacctgcga cctcatgctc cggaggcatg cgctctatcc agctgagcta cgggcgcgga
      781 agcgctgagt ctagtgatcg ggctagggtg cgggcatgga cctctccgtc gcatttcttg
      841 gcactggcgg tccggtgcca accgccctgc gcggcaccgc cagcgtgctg atcacgcgcg
      901 gcggagaaaa actcatgttc gactgcggcg agggaaccca gcggcagatg cggcgatcga
      961 tggggctggt ccaggtcgac gagatctacc tgactcactt ccacctcgat cactgcctcg
     1021 gcctgcccgg tctgctcaag acctatgacc tgaatgaccg cgaggagccg ctcatgctct
     1081 tcggcccggt cggccttcac gggctgatga aggacctgca gccgctgatc ggccggctcg
     1141 attaccacct cgaggtcgag gagctcgaag gcggagacgt ggttccccac gtcgattacg
     1201 agatcgagtg tttcccggtc gaacaccgca cccgggcgct cggctacgcc ctcctcgagg
     1261 atgaccgccc cggccgcttc gaccccgcag aggcggagcg gctcggcgtg aaggccgggc
     1321 ccgacttcaa gaggctccag gacggggag
//
LOCUS       DAOQMF010000629         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_83196_length_1348_cov_3.129157, whole genome shotgun sequence.
ACCESSION   DAOQMF010000629 DAOQMF010000000
VERSION     DAOQMF010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83196_length_1348_cov_3.129157"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..729
                     /locus_tag="PLV77_09670"
     CDS             163..729
                     /locus_tag="PLV77_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16158.1"
                     /translation="MAVVLAGTLILAGCGGGDSDSGGSYGSSDNGSSAGTGYGNEAGS
                     QSADSTGKSDYGSNGSGAAGGSMAAGAAVVSAANVGDLGKVLVDDKGMTLYMFEKDKG
                     GKSACYGGCAEAWPPLATDGDPVAKNGAVAGKLGTTGRTDGSTQVTYAGWPLYLYAGD
                     QKPDQAKGNDIDQFGAEWYALTPSGEKP"
     gene            797..1288
                     /locus_tag="PLV77_09675"
     CDS             797..1288
                     /locus_tag="PLV77_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC16159.1"
                     /translation="MREAYRAHSGELFGFACRSLGDRDRAEEAVQECFLKAWRSRASY
                     DPERGSERTWLFAILRNVIADLGRSDGRRPAGAPLEESADRIPSTDQPFERAMLAWQV
                     EEALRRIGERHRQIVVETCLRGRPYAEVAAALGIPEGTIKSRVYYGLRAMRDAMEELG
                     YES"
BASE COUNT          225 a          425 c          477 g          221 t
ORIGIN      
        1 ccatatagcg ctccattctg cctcctcgct gggcgctagc ggtacgcgtt cctggttccg
       61 cagactacga tcctggccgc agccggtgaa ccttttcggg gccgggtcgt atcccctcac
      121 atgaactctt catggatttt ggatcgcagg ggcctggcga ctttggcggt cgttctggcc
      181 ggaaccctga tcctggccgg ctgcgggggc ggcgactcgg attccggcgg ctcttacggc
      241 tcgtccgaca atggatcgag cgccgggacc ggttacggca acgaggccgg atcacagtcg
      301 gcggattcga ccggcaaatc ggattacggt tcgaacggtt ccggcgcggc gggcggttcg
      361 atggctgccg gtgcggcggt cgtttcggcg gcgaacgtcg gtgacctcgg caaggtgctg
      421 gtcgacgaca aggggatgac cctttacatg ttcgaaaagg acaagggcgg aaagtcggcc
      481 tgctacggcg gctgcgccga ggcatggccg cccctggcca ccgacggaga tccggtcgcc
      541 aagaatggcg ccgtggcggg caagctcggc acaaccgggc ggaccgacgg ctcgacccag
      601 gtgacctacg ccggctggcc gctttacctc tacgccgggg atcagaagcc ggaccaggcg
      661 aagggcaatg acatcgacca gttcggtgcc gagtggtacg ccttgacccc gagtggcgaa
      721 aagccctgag acggcggacc ggacagttag tttctgacca ggaggcaacc gaccagtgac
      781 gatgccgggt gaacaggtgc gcgaggccta ccgggctcac tccggtgagc tcttcggctt
      841 cgcctgccgt tcactcggcg accgggaccg ggcggaggaa gccgtccagg agtgtttcct
      901 caaggcctgg cgcagccgcg cgagctacga cccggaacgg ggctcggaaa ggacctggct
      961 cttcgcgatc ctccgcaacg tgatcgccga cctcggtcgg tccgatggcc gacggccggc
     1021 cggcgctccg cttgaggaat ccgccgaccg gatcccttca accgaccagc cgttcgagcg
     1081 ggcgatgctg gcctggcagg tcgaggaggc actccgccgg atcggtgaaa ggcaccgcca
     1141 gatcgtggtc gagacctgtc tgcggggtcg gccctatgcc gaggtcgcgg cggcgctcgg
     1201 catccccgag ggcacgatca agagccgggt ctactacggg ctgcgggcga tgcgggacgc
     1261 gatggaggaa cttggctatg agtcctgagc accgacagat ccgtgaaagc ctcggtgcct
     1321 gggcggttgg ccacctcgac cccgatga
//
LOCUS       DAOQMF010000630         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_83433_length_1346_cov_3.900852, whole genome shotgun sequence.
ACCESSION   DAOQMF010000630 DAOQMF010000000
VERSION     DAOQMF010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83433_length_1346_cov_3.900852"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLV77_09680"
     CDS             <1..1341
                     /locus_tag="PLV77_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNC16160.1"
                     /translation="STRREGDGLRLWIHIADVSAFVRPGSILEKEAFLRANSTYVPTS
                     VEPMLPHSLSSGVCSLSPGDERLAVTSEMLLSGSGEVTSASFYRSRIRSDARLDYDQL
                     DQIFAGRESAPEIVAEQLDLARKAAAILAELRKGNALEVNSAEPSFEFDDEGNVTRAV
                     AERATEAHRLIEQLMVLTNEQVAGLTVNRGIPSVYRVHEMPDPDRVERMLAQLASLEL
                     PAPPVPSGMGPSTAGQLAVEASRSVAKEAARRGHGATAYSSLVLRSLKPARYLEKNLG
                     HAGLGSEAYCHFTSPIRRFPDLLVHRALLSAVGGGEEAPSTGDAAEAALHASEREREA
                     MILERDADDICAAFLLERELFQEGYEKRFEGEVSGVIRSGAFVRFNGRLGDVYEGFIP
                     ARRIRGERFELNEEETSLVGSKTGRKVSLGDAIAVQVEKVDAPRGRVDLDPADG"
BASE COUNT          226 a          439 c          463 g          218 t
ORIGIN      
        1 tctacccgcc gcgaggggga cgggctgcgg ctctggattc acatcgccga cgtatccgcc
       61 ttcgttcgcc cgggttcgat tctcgaaaag gaagcctttc tgcgggccaa cagcacctat
      121 gtaccgacct cggtcgaacc gatgcttccg cacagcctca gttccggcgt ctgctccctt
      181 tcgcccggcg acgagcggct ggccgtcact tcggagatgc tgctctcggg aagcggcgag
      241 gtaacttcgg ccagtttcta ccgcagccgg atccgctccg atgcccggct tgactacgac
      301 cagctcgacc agatcttcgc cgggcgcgaa agcgcccccg aaatcgtcgc cgaacaactg
      361 gacctggccc ggaaggcggc agcgatcctg gccgaactcc ggaaggggaa cgcgctcgaa
      421 gtcaactcgg ccgagccttc cttcgaattc gatgacgagg gcaacgtgac cagggcggtg
      481 gccgagcggg caaccgaagc gcaccggctg atcgagcagc tgatggtcct gaccaacgaa
      541 caggtggccg ggctgactgt gaaccgcggc atcccctccg tctaccgcgt ccacgagatg
      601 cccgaccccg accgggtgga acggatgctc gcccagctgg cctcgctgga gttgccggcg
      661 ccgccggtcc cctccggcat ggggccgagc acggccgggc agctggcggt ggaggcttcc
      721 cgttcggtcg cgaaagaggc ggcgcggcgg ggccacgggg ccacggccta cagctcgctg
      781 gttctccggt cgctgaagcc ggcccgctac ctggagaaga acctcggcca cgccgggctg
      841 ggcagcgagg cctactgcca cttcacctcg ccgattcgcc gttttcccga cctcctggtc
      901 caccgggcgc tgctttcggc ggtcgggggc ggagaggagg ctccctcgac cggcgacgcc
      961 gcggaagctg cgctccacgc ttcggagcgt gaaagggaag cgatgatcct cgaaagggat
     1021 gccgatgaca tctgcgccgc tttcctgctt gaacgggagc tgtttcagga gggatacgaa
     1081 aagcgtttcg agggggaggt ctccggggtg atccgctccg gcgccttcgt ccggttcaac
     1141 gggcggctcg gcgatgtcta cgagggattc attcccgccc ggcggatccg gggtgagcgg
     1201 ttcgaactga acgaagaaga aacttcgctg gtcggcagca agaccgggcg caaggtcagc
     1261 ctcggcgatg cgatcgcggt ccaggtcgaa aaggtggatg caccgcgggg gagggtggat
     1321 ctcgacccgg cggatggctg aaagct
//
LOCUS       DAOQMF010000631         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_83610_length_1345_cov_2.331008, whole genome shotgun sequence.
ACCESSION   DAOQMF010000631 DAOQMF010000000
VERSION     DAOQMF010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83610_length_1345_cov_2.331008"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLV77_09685"
     CDS             <1..334
                     /locus_tag="PLV77_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16161.1"
                     /translation="PNLDQRQMIKTVKSLHAGTEGVKAELMLDHAERILKGAGGERWH
                     GGGGIWLIATFGNGKRITAAPDEEGAATYSIEVTDEETGESLYVKDALTPSALTDEWL
                     TQTVKHTM"
     gene            383..568
                     /locus_tag="PLV77_09690"
     CDS             383..568
                     /locus_tag="PLV77_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16162.1"
                     /translation="MSDQEILEAIFLLDDDAPAMDIANEIGAPVPDVKARLQELTRTG
                     MVDQTGNAFGLTDVGMD"
     gene            620..1282
                     /locus_tag="PLV77_09695"
     CDS             620..1282
                     /locus_tag="PLV77_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16163.1"
                     /translation="MGKEMFASVLWLPESQEPNWPAAQRKAQEIIWDENTEDSELTET
                     GIYSDVEYAAHEARRLGRPHDPNYHLQGLLRDLIGQVEKAVGGENREMLELNQYGWRV
                     FITGGVDDNETELGEALETLNRLDILAAAGFNPPDQMIETVVYENRHGTSVCVLKDGL
                     GVAQAASIAREGWDEISHIREVPTSPPGDDSEALSIFTEFAGEGGCWINRERLPIQHR
                     VD"
BASE COUNT          332 a          439 c          405 g          169 t
ORIGIN      
        1 gccgaacctg gaccagcggc agatgatcaa gaccgtcaag agcctccacg ccggcaccga
       61 aggcgtcaag gccgagctga tgcttgacca tgccgaacga atcctcaaag gagccggcgg
      121 cgagcgttgg cacggcggcg gcgggatctg gctgatcgcc acgttcggaa acggcaaacg
      181 catcacggcc gcaccggacg aggagggggc agccacctac tcgatcgagg tcaccgacga
      241 agaaaccggc gaaagcctct acgtcaagga cgccctgacg ccgtcggcgc tcaccgacga
      301 gtggctcacc cagacggtca aacacacgat gtagcagggc ccgcaagggc caccgaaaac
      361 ggccacagac aaggagacac acatgagcga ccaggaaatc ctcgaggcga tctttctgct
      421 tgacgacgac gccccggcaa tggatatcgc caacgagatc ggtgccccgg tcccggacgt
      481 gaaagcccgc cttcaggaac tcaccaggac cggaatggtg gaccagaccg gcaacgcgtt
      541 cggcctgacc gacgtcggca tggactagct cgagcagctg accgcctgaa cccccgaaca
      601 caagaaagga gaagagacga tgggaaagga aatgtttgcc tcggttctct ggctgcccga
      661 aagccaggaa ccaaactggc cggccgccca aaggaaagcc caggaaatca tctgggacga
      721 aaacaccgag gacagcgagc tcaccgaaac cgggatctac agcgacgtcg aatacgccgc
      781 acacgaagcc cggcgcctcg gccgtcccca cgacccgaac tatcacctcc agggactcct
      841 gcgagacctg atcggccagg tcgagaaagc cgttggcggt gagaaccggg aaatgctgga
      901 actcaaccag tacggctggc gagtattcat caccggcggc gtcgacgaca acgaaaccga
      961 actgggagag gcactcgaaa cgctcaaccg gctcgacatt ctcgccgccg ccggattcaa
     1021 cccgcccgac cagatgatcg aaacagtcgt ctacgagaac cgtcacggaa cgtccgtgtg
     1081 cgtactcaaa gacgggctcg gcgtggccca ggccgcatcg attgcccggg aaggctggga
     1141 tgaaatctcg cacattcgcg aggttccgac gagtcctcca ggtgacgaca gcgaagccct
     1201 gtcgatcttc accgagttcg ccggcgaagg cggctgctgg atcaaccgtg aacggttgcc
     1261 gatccagcac cgggtcgact agcagcacct gaagcaccgt cgcgggacgg cctcccgcac
     1321 gacaaagcac ccgtcgcccg atcct
//
LOCUS       DAOQMF010000632         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_84618_length_1337_cov_2.290952, whole genome shotgun sequence.
ACCESSION   DAOQMF010000632 DAOQMF010000000
VERSION     DAOQMF010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84618_length_1337_cov_2.290952"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLV77_09700"
     CDS             <1..217
                     /locus_tag="PLV77_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16164.1"
                     /translation="MYRFKFFGKEVVTLLILLALSLNFSVIASRQSKQITRLAQQQAI
                     DGVEEEAASPAASSRGETPAPREQAPR"
     gene            complement(156..1256)
                     /locus_tag="PLV77_09705"
     CDS             complement(156..1256)
                     /locus_tag="PLV77_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16165.1"
                     /translation="MTTLAEATSHQPLAAAELDAALRHGPSHAYLFQGPSGSGKAKAA
                     RSFAAEILSRSSENPEDTRRRVLLDPSPHPDLVWLKPKGMSHAIREIRDRVIHQAPLS
                     PFEGSARVFVIEAADALNEESQNAMLKTLEEPAPHAHLILLSAESEGLLPTIASRCQL
                     IEFGSLPFEVIEAELDGLASPEALHSAAKLAGGDLDKARFLAGKRGSDLRVAAERLMT
                     CALEDEFPGSPWLDLLAQARSAGEHSGELVQADFDEEKEEGIKHTKTEIEEAVRRAQR
                     KTRTGMLDLGLHLAAAWARDWATVIAGAPDLAFNQDRLEVLEAQVEGIELGQARDAVS
                     QIQETRRSFQLNVSEELALEALAFRLEKTLRD"
BASE COUNT          194 a          435 c          464 g          244 t
ORIGIN      
        1 gatgtaccgc ttcaagttct tcggcaagga ggtggtgacc ctgctgatcc tgctcgccct
       61 ctcgctcaac ttttcggtga tcgcttcccg ccagtcgaag cagatcacac ggctcgccca
      121 gcaacaggcg atcgacgggg tcgaggaaga agcggctagt cccgcagcgt cttctcgagg
      181 cgaaacgcca gcgcctcgag agcaagctcc tcgctgacgt tcagctggaa gctgcggcgg
      241 gtttcctgaa tctggctgac cgcgtcacgg gcctggccga gttcgatccc ttccacctgg
      301 gcctcgagga cttcgagccg gtcctggttg aaggcgaggt cgggggcgcc ggcgatgacc
      361 gtcgcccagt cacgggccca ggcggcggcg aggtgaaggc cgaggtcgag catgccggtg
      421 cgggtcttcc gctgggccct tcgcaccgcc tcctcgatct cggtcttggt gtgcttgatg
      481 ccctcttcct tttcctcgtc gaagtcggcc tggacgagct cgccggaatg ctcgcccgcc
      541 gacctggcct gggccagcag gtcaagccag ggcgagccgg ggaactcgtc ttcgagggcg
      601 caggtcatca gccgttcggc cgcgaccctg aggtcgctgc cccgtttgcc ggccagaaac
      661 cttgccttgt cgaggtcccc gccggccagt ttggcggccg agtggagcgc ctcgggactt
      721 gccagcccgt ccagttccgc ttcaatcacc tcgaacggga gcgacccgaa ctcgattagc
      781 tggcagcggg aggcgatggt cggcagcagg ccttcgcttt cggctgaaag caggatcagg
      841 tgggcatgcg gcgccggctc ttcgagggtc ttcagcatcg cgttctggct ttcctcgttc
      901 agggcgtcgg ccgcctcgat cacgaagacc cgggccgagc cctcgaaggg actgagcggt
      961 gcctgatgga tcacccggtc gcggatctcg cggatcgcgt gactcattcc cttcggtttg
     1021 agccagacca gatccggatg cggcgaaggg tcgagcagca cccgtcggcg ggtgtcttcc
     1081 gggttctcgc ttgagcggct caggatctcg gcggcgaaac tgcgggcggc cttcgccttg
     1141 ccggaaccgg atggcccctg gaagaggtag gcatgggacg gaccgtgccg aagggcggcg
     1201 tcgagctcgg ccgcggcaag gggctggtgg gaggtcgcct cggccagcgt ggtcatgccg
     1261 ggcgccccgc tgcaggactt ccggcgatca caccggcagc ctgggttcga cctcggccat
     1321 gatcagccgg tggacct
//
LOCUS       DAOQMF010000633         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_85119_length_1333_cov_2.448357, whole genome shotgun sequence.
ACCESSION   DAOQMF010000633 DAOQMF010000000
VERSION     DAOQMF010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85119_length_1333_cov_2.448357"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLV77_09710"
     CDS             complement(<1..396)
                     /locus_tag="PLV77_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNC16166.1"
                     /translation="MTAETKTLSTYIQDRIDEFSRSQKDVARYIVDHLDEAAFLTAEE
                     LARRAGTSSSTVVRFSQALGFEGYPELQASAIEEYRATVGGEESGGRMLFSFDHSEFE
                     ASLGTDHSNLEETARKLDQSQIDEAVAALS"
     gene            465..800
                     /locus_tag="PLV77_09715"
     CDS             465..800
                     /locus_tag="PLV77_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16167.1"
                     /translation="MNQFQGLPNRKTSLTGHTDEGDEIWIIRSISQKFYKCLGCKGEI
                     NIGDEHVVVQYVGKYGGTEHSHWHQRCAEEILFYQVRGMRKVSSKESSRDRLESRGRR
                     PAGRRRRPR"
     gene            complement(856..>1333)
                     /locus_tag="PLV77_09720"
     CDS             complement(856..>1333)
                     /locus_tag="PLV77_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HNC16168.1"
                     /translation="NVKGWKATSAGNTLTWTGGPLPDHQTQDFPFTVTLYGKKDENVM
                     FKTIQKCQGDASTAWIQAMEGDVEPEYPAPAVTLTSTAKAQVSPATPADQQSDDDQVA
                     EGDAAATGEPTATAADAEDSSSDDEGSDSKTLILIIIAGLAIGTVAGIVVRARRKQ"
BASE COUNT          230 a          407 c          437 g          259 t
ORIGIN      
        1 ggaaagggcg gcgaccgctt cgtcgatctg gctctgatcc agcttgcggg cggtttcttc
       61 gaggttcgag tggtcggtgc cgagggaagc ttcgaattcg gagtgatcga aggaaaagag
      121 catgcggccg ccggactctt ccccgcccac ggtggcgcgg tactcctcga tcgcagaggc
      181 ctggagttcg gggtagccct cgaatccgag cgcctgggag aagcggacaa ccgtcgagga
      241 ggacgtgcct gcccgacggg ccagttcctc cgcggtcagg aaggccgctt catcgagatg
      301 gtcgacgatg tagcgcgcga catccttttg ggagcgggaa aactcgtcga tccgatcctg
      361 gatgtaagtt gacagggtct tggtttcggc ggtcactggg ctcacgccgg atcaattcgc
      421 cccgcccgtt caattccctt ctaaattcgc tctgagcagg acttttgaat cagttccagg
      481 gacttccgaa ccgcaagacc tcactcaccg gacacaccga cgagggtgac gagatctgga
      541 tcatccgctc gatctcgcag aagttctaca agtgcctcgg ctgcaagggg gaaatcaaca
      601 tcggggacga gcatgtggtc gtccagtacg tcgggaagta cggcggcacc gagcattcgc
      661 actggcatca gcgctgcgcc gaggagatcc tcttctacca ggtgcgtggc atgcgaaagg
      721 tctcctcgaa agagtcaagc cgggaccggc tcgaaagccg gggccgccgg cctgccggac
      781 gcaggcgacg tccccgctga actttctgca aaacgtcgcg ggcgcgctga acttcatgct
      841 tcccgcggct ggcccttact gcttgcggcg ggcccgaacc acgatgccgg cgaccgtgcc
      901 gatcgccagc ccggcgatga tgatcaggat cagggtcttg ctgtccgagc cctcgtcatc
      961 ggaggacgag tcctcagcgt cggccgcggt cgcggtcggc tcaccggtcg cggcggcgtc
     1021 gccttcggcg acctggtcat cgtccgactg ctgatcggcc ggagtggcgg gtgacacctg
     1081 ggccttcgcg gtcgatgtga gggtcacggc cggggccggg tactccggtt cgacgtcacc
     1141 ttccatcgcc tgaatccagg cggtcgaggc gtcgccctga catttctgga tcgtcttgaa
     1201 catcacgttc tcgtccttct ttccgtagag ggtcaccgtg aaggggaaat cctgagtctg
     1261 gtgatcgggc aacggaccgc ccgtccaggt cagggtgttg ccagcggaag tcgccttcca
     1321 gcccttgacg ttt
//
LOCUS       DAOQMF010000634         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_85248_length_1332_cov_2.364918, whole genome shotgun sequence.
ACCESSION   DAOQMF010000634 DAOQMF010000000
VERSION     DAOQMF010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85248_length_1332_cov_2.364918"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLV77_09725"
     CDS             complement(<1..545)
                     /locus_tag="PLV77_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC16169.1"
                     /translation="MSEGFTHVFEPGDPARTLLLLHGTGGDENDLVSLGRQLAPGAGI
                     LSPRGKVLENGMPRFFRRLAVGELDIPDLLVRTDELADWVTTAAGDYGFDPAGLIGLG
                     LSNGANIAASLLFRRPGTLGAAALLRPMLPYPPPEDLDLQGAPVLVASGGQDQFVPAG
                     ESEELARQLEAHGAKVEFIFDP"
     gene            643..>1332
                     /locus_tag="PLV77_09730"
     CDS             643..>1332
                     /locus_tag="PLV77_09730"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC16170.1"
                     /translation="MIKGVLAVSLASLLVLAGVARADQGTEWIPPLDAVARDKGEMAF
                     DGCRPKPERIDPVMCVYGDPDSDKTIALFGDSHAMEWGPPLIRLAQRRGWRLVTFLRA
                     GCVIADVKFQNNCDEWRELAFQEIAEQRPNRVIVSTSIGRRYTLSVRGETLSRTESEK
                     ILRRGMARTLKRLASIRSLTPHGTKVKLIRDQITAPFLPPRCLADHPDHPRLCRFPRK
                     RKFGPGFDVVGA"
BASE COUNT          226 a          436 c          450 g          220 t
ORIGIN      
        1 ggatcgaaga tgaattcgac cttcgcgccg tgggcctcga gctggcgggc gagctcctcg
       61 ctttccccgg cgggcacgaa ctggtcctgg ccgccggaag cgaccagaac gggcgccccc
      121 tgcaggtcaa ggtcctccgg tggcgggtag gggagcatcg gtctgagcag agccgcggca
      181 cccagggtgc cgggccgccg gaagagcagg ctggcggcga tgttggcgcc gtttgaaagc
      241 ccgaggccga tcagcccggc cgggtcgaag ccgtaatccc cggcggccgt ggtcacccag
      301 tcggccagct cgtcggttcg caccagcagg tcggggatgt cgagctcccc gactgccagc
      361 cgccggaaga agcggggcat tccgttctcg agaaccttgc cgcgcgggct gaggatgcct
      421 gcccccgggg cgagctggcg accaagcgaa acgaggtcgt tctcgtcacc gccggtgccg
      481 tgaagcagca ggagggtccg ggccgggtcg ccaggttcga agacgtgagt gaagccctcg
      541 gacatggatc cactctaaat gagtgtctgg cccggccgaa acccgggccg aaaacgtgtg
      601 tttgatggtc accagtccat tccagtccac agggagatca agatgatcaa gggagttctc
      661 gcggtttcac tggcctcgct tctcgtcctc gccggcgttg cccgcgccga tcagggcacc
      721 gaatggattc cgccgctgga cgcggtcgcc agggacaagg gcgagatggc ctttgacggc
      781 tgccggccca agccggagcg gatcgatccg gtgatgtgcg tgtacgggga tccggattcc
      841 gacaagacga tcgccctgtt cggtgattca catgcgatgg agtggggtcc gccgctgatt
      901 cgcctggccc agcgacgcgg ctggcggctg gtcaccttcc tgcgggctgg atgcgttatc
      961 gccgacgtca agttccagaa caactgcgac gagtggcggg aactcgcctt tcaggagatc
     1021 gccgaacagc gacccaaccg ggtcatcgtc tcgacctcga tcggtcgccg ctacacgctc
     1081 tcggtacgcg gcgagaccct ctcccggacc gaaagcgaaa agatcctgcg ccggggaatg
     1141 gcccgcaccc tgaagcgtct ggcgagcatc cgcagcctga ctccgcacgg aacgaaggtg
     1201 aagctgatcc gcgaccagat aaccgctcct ttccttcctc cgcgttgtct ggccgatcat
     1261 cccgaccacc cccgtctctg ccggttcccc cgcaaacgca agttcgggcc cgggtttgac
     1321 gtggtcggcg cg
//
LOCUS       DAOQMF010000635         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_85498_length_1330_cov_2.238431, whole genome shotgun sequence.
ACCESSION   DAOQMF010000635 DAOQMF010000000
VERSION     DAOQMF010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85498_length_1330_cov_2.238431"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..751)
                     /locus_tag="PLV77_09735"
     CDS             complement(8..751)
                     /locus_tag="PLV77_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNC16171.1"
                     /translation="MIGPTDVEIRVLGCLIEKQRTTPDQYPLTLNSLRLACNQSTNRE
                     PVVDYDEAEIRKALGEPGRRRWIRGASGHTSRSPKYRHLLDQELSLSEDQISVLAVLM
                     LRGEQTPGQLKQRTERMFGFHDLDALQTPLDELSSRELIERLPRRPGQKEDRYRQLLG
                     GSHLEDEAAEQAGHPPFEAETSGGDEPPNREANAPGTPVPAAEPLPVPVDTPETERIE
                     TLERQIAGLNGELAELRGLVDELRRELGV"
     gene            complement(748..>1330)
                     /locus_tag="PLV77_09740"
     CDS             complement(748..>1330)
                     /locus_tag="PLV77_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNC16172.1"
                     /translation="LLCEAAGFDVIIVESVGVGQSEAELAEMVDCLMLLLVPGAGDEL
                     QGIKRGIMELADLIVVNKADGDRVALAKRARGDYRHALRMLPPSTPGWETPVQIASAA
                     EGTGLKEIWESVEAHRALLEESGLLHERRRQQTERWLDSMIEEAVLAAVHSRQGVDAT
                     IKQARIDVDAEKITVPQATRKVIEAAGLAGEQK"
BASE COUNT          176 a          459 c          458 g          237 t
ORIGIN      
        1 gtggaactca gaccccgagt tcccgccgga gctcgtcaac cagtcccctg agctcggcca
       61 gctcgccgtt tagcccggcg atctggcgct ccagcgtttc gatccgctcg gtttcaggcg
      121 tatcgaccgg gaccggcagc ggttctgccg ccgggaccgg cgttccgggg gcgttcgcct
      181 cccgattcgg tggttcatcg cccccggaag tttcagcctc gaaagggggg tggccggcct
      241 gctccgcggc ttcgtcttcg aggtggcttc cgccgagcag ctggcggtag cggtcttcct
      301 tctggcctgg ccggcggggc aggcgctcga tcagctcgcg cgaggaaagc tcgtcgaggg
      361 gggtctgcag cgcgtcaagg tcgtggaaac cgaacatgcg ctcggtccgc tgcttgagct
      421 ggcccggggt ctgctcgccg cgcagcatca gcaccgcaag caccgagatc tggtcctcgg
      481 atagcgaaag ctcctggtcg agcaggtgcc ggtacttggg cgagcggctg gtgtggcccg
      541 aggcgccccg gatccagcgc cggcggccgg gctcgcccag cgccttgcgg atctccgcct
      601 cgtcgtaatc gacgaccggc tcccggttgg tcgactggtt gcaggccagc cgcagcgagt
      661 tcagggtcag cgggtactgg tccggggtcg tccgctgctt ctcgatcagg cagccgagca
      721 cgcggatctc gacatcggtc ggtccgatca tttctgctcc cctgcgagac cggcggcttc
      781 gatcaccttg cgggtggcct gggggacggt gatcttctcg gcgtccacgt caatgcgggc
      841 ctgtttgatc gtcgcgtcca ccccctgccg ggaatggacg gcggcgagga cggcttcctc
      901 gatcatcgaa tcgagccagc gctcggtctg ctggcggcgg cgttcgtgga gcaggccgct
      961 ttcctcgagc agtgcccggt gggcctcgac cgattcccag atctccttga gtccggtgcc
     1021 ctcggcggcc gaggcgatct ggaccggggt ctcccagccg ggagtcgagg gcggcagcat
     1081 gcggagcgcg tggcggtagt caccccgggc ccgcttggcc agcgccaccc ggtcaccgtc
     1141 ggccttgttg accacgatca ggtcggccag ttccatgatg ccccgcttga tcccctgaag
     1201 ctcgtcgccg gcgccgggaa ccagtagcag catcaggcag tcgaccatct ccgccagctc
     1261 cgcctcggac tggccgaccc cgaccgactc gacgatgatc acgtcgaaac cggccgcctc
     1321 gcagagcagc
//
LOCUS       DAOQMF010000636         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_85720_length_1328_cov_2.737628, whole genome shotgun sequence.
ACCESSION   DAOQMF010000636 DAOQMF010000000
VERSION     DAOQMF010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85720_length_1328_cov_2.737628"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLV77_09745"
     CDS             complement(<1..432)
                     /locus_tag="PLV77_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HNC16173.1"
                     /translation="MAKITDDTKEQVRAAADMVEIVSAHTDLKRAGGGRYMGLCPFHE
                     ERSPSFSVNNNDKLYHCFGCGVGGDIFDFVMSIENVDFPTALEQLADRYGVELKREDE
                     DPRAAQRRQEMARMTELMDRAGAFYSNFFQTADEAGKAREYL"
     gene            complement(452..>1328)
                     /locus_tag="PLV77_09750"
     CDS             complement(452..>1328)
                     /locus_tag="PLV77_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNC16174.1"
                     /translation="RDRDRIVHSKSFRRLMHKTQVFIAPEGDHYRTRLTHTLEATGIA
                     RTVARALRLNEDLTEAIGLAHDLGHPPFGHIGEQALDEASRSHGGQGFKHNENSLRVV
                     DVLEREGRGLNLNQPVREGILKHTGPVKSESLEGQIVRLVDRVAYINHDIDDALRAGI
                     LSSEDLPNEEIAMLGPTGASRIDSLVKDIVNQSRDAGEIVQSEHMGMAMLRLRKFMFD
                     NVYLGGEARKDHDRVHGVIRALFEHYLERIEEVPVLDPGADPIQRVTDLLAGMTDRYC
                     IAIFTSIYVPEGSRF"
BASE COUNT          217 a          411 c          416 g          284 t
ORIGIN      
        1 caggtattcc ctggccttcc cggcctcgtc tgcggtttgg aaaaagttcg agtagaaggc
       61 gccggcccgg tccatcagtt cggtcattcg ggccatctct tggcggcgct gtgccgctcg
      121 cggatcctcg tcttcccgtt tgagctcgac tccgtagcgg tcggccagtt gctcgagcgc
      181 ggtcggaaag tcgacgttct cgatgctcat cacgaagtcg aaaatgtcgc ctccgactcc
      241 gcaaccgaag cagtggtaga gcttgtcgtt gttgttgacc gaaaacgacg gcgaccgttc
      301 ttcgtgaaag gggcatagcc ccatgtagcg accgccgccg gctcttttca ggtccgtgtg
      361 ggcggagacg atttccacca tgtccgcggc ggcacgaacc tgctccttgg tgtcatcggt
      421 gatcttcgcc atcgcggtcg agtctacgaa ctcagaaccg ggaaccttcc ggcacgtaga
      481 tcgacgtgaa gatggcgatg cagtagcggt cggtcattcc ggccaacagg tcggtgaccc
      541 gctggatcgg atcggcccct ggatcaagca ccggcacctc ctcgatccgc tccagatagt
      601 gttcgaagag ggcccggatt accccgtgaa cccggtcgtg atccttgcgg gcctcgccgc
      661 cgagatagac gttgtcgaac atgaacttgc gcagccgcag catcgccatg cccatgtgct
      721 cgctctgaac aatttcgccg gcgtcccggg actggttgac gatgtctttg accagggaat
      781 cgatccggct ggcgccggtc gggccgagca tcgcaatctc ctcgttcggg aggtcttcag
      841 aactgaggat cccggcccgc agagcgtcgt cgatgtcgtg gttgatgtag gcgacacgat
      901 cgaccagccg aacgatctgg ccttcaaggg actcgctctt gaccggtcca gtgtgcttca
      961 gaattccctc gcgcaccggc tggttcaggt tcagtcctcg gccttcgcgt tcgagcacgt
     1021 cgaccacccg cagcgagttc tcgttgtgtt tgaaaccctg cccgccgtgg ctccggctcg
     1081 cttcatcgag tgcctgttcg ccgatgtgac cgaagggtgg gtgaccaagg tcatgcgcca
     1141 ggccgatcgc ctcggtcaga tcctcgttca gtctcagggc ccgggccact gtgcgggcga
     1201 tcccggttgc ctcgagagtg tgggtgagcc gggtccgata gtggtcgcct tcgggcgcga
     1261 tgaaaacctg ggtcttgtgc atcagccgcc ggaacgactt gctgtggacg atgcggtcac
     1321 ggtcgcgc
//
LOCUS       DAOQMF010000637         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_85726_length_1328_cov_2.538099, whole genome shotgun sequence.
ACCESSION   DAOQMF010000637 DAOQMF010000000
VERSION     DAOQMF010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85726_length_1328_cov_2.538099"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLV77_09755"
     CDS             <1..785
                     /locus_tag="PLV77_09755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC16175.1"
                     /translation="ALGIYGMGNIGTAVAAFSIPAIRDATSEGVAGLVFAAVIGVYAL
                     IWMSMAREAPVEKAPPTRYREVLGAGWKLWRLALFYFVTFGGFVAMALYLPKLLVDWY
                     DLSLTDAGLRAAGFTLLATAIRPVGGMLSDRIGGNRVLAVAFLGIGIDAVGLSWQASV
                     PTMWLTTVFCLTMALFLGLGNGAVFKLVPLTFPNATGAATGIVGAVGGLGGFFPPLFL
                     GIVKDATGDFVLAFVMLVAFAWLCAGLAFEGRVPDSGTNGPD"
     gene            complement(868..>1328)
                     /locus_tag="PLV77_09760"
     CDS             complement(868..>1328)
                     /locus_tag="PLV77_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNC16176.1"
                     /translation="GRITEKEFQVQLADGLEPLLGHRPEIEDFGLLLFEALDPNPGMI
                     DLIREVRRDGIRTSLLTNNVKEWEVKWRSMMPIDELFETVVDSAFVGCRKPDPRIYNL
                     TLERVGLDPEECIFIDDMKINIDAANELGLHGVHFRETAQVRAEVHDLLA"
BASE COUNT          191 a          438 c          430 g          269 t
ORIGIN      
        1 tcgcgctcgg catctacggc atgggcaaca tcggcaccgc ggttgccgcc ttctcgattc
       61 cggcgatccg cgacgccacc agtgaaggtg tggcgggact cgtcttcgcc gcagtgatcg
      121 gcgtctacgc gctgatctgg atgtcgatgg cccgggaggc accggttgaa aaggcgccgc
      181 cgacccgcta ccgcgaggta ctcggcgccg gctggaaact ctggcgactg gcgctctttt
      241 acttcgtgac cttcggtgga ttcgtcgcca tggcgctata cctgccgaag ctgctggtcg
      301 actggtatga cctttcgctc accgatgccg ggttgcgcgc cgccggcttc acgctgctgg
      361 caaccgcgat caggccggtc ggcggcatgc tctcggaccg gatcggtggc aaccgcgtac
      421 tggcggtcgc cttcctcggg atcggcatcg acgcggtcgg cctctcctgg caggcctcgg
      481 ttccgacgat gtggctgacc accgtcttct gcctgaccat ggccctcttc ctcggtctcg
      541 ggaacggcgc cgtgttcaag ctggtgccgc tcactttccc gaacgcaacc ggtgccgcaa
      601 ccggcatcgt cggcgccgtg ggtggcctcg gcggcttctt cccgcccctg ttcctgggaa
      661 tcgtgaagga cgcaacgggt gacttcgtgc tcgccttcgt gatgctggtc gccttcgcct
      721 ggctgtgtgc cggcctggcc ttcgagggcc gggttcccga ctcggggacg aacggcccgg
      781 actagtctga gccacttcgg tacggcgccc ctcccgacct ccgagcggga ggggctccgc
      841 ccgaagtaaa gccaggcgaa accctgttta cgccagcagg tcgtgcactt cggcccggac
      901 ctgtgcggtt tcgcggaagt gaaccccgtg gagcccgagt tcgttcgcgg catcgatgtt
      961 gatcttcatg tcgtcgatga agatgcattc ctccgggtcg aggccgaccc gttccagggt
     1021 caggttgtag atccgcggat ccggcttgcg gcagccgacg aaagccgagt cgaccacggt
     1081 ctcgaagagc tcgtctatcg gcatcatcga gcgccatttg acttcccact ccttgacgtt
     1141 gttggtcaga agtgaagtgc ggatgccgtc ccggcggacc tcccggatca ggtcgatcat
     1201 gcccgggttc gggtcaaggg cttcgaagag cagcaggccg aagtcctcga tttccggccg
     1261 gtggccgagc aacggctcga ggccatccgc cagctggacc tgaaattcct tttcggtgat
     1321 ccggccac
//
LOCUS       DAOQMF010000638         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_86076_length_1325_cov_3.693701, whole genome shotgun sequence.
ACCESSION   DAOQMF010000638 DAOQMF010000000
VERSION     DAOQMF010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86076_length_1325_cov_3.693701"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /gene="era"
                     /locus_tag="PLV77_09765"
     CDS             <1..722
                     /gene="era"
                     /locus_tag="PLV77_09765"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNC16177.1"
                     /translation="LPGVQRPRDELTSRMQKTVESELAGADVAPLVINGEEGVGPGDR
                     FIAETLLGARAKAGGLPIICAVNKADRLGRNIVPVLVAAAELAGVDEVFPVSALTGQG
                     VPELASYLASIVPEGPYLYPPEDHSDQPRELLLAELIRGQALIRTRDEIPHSVEVRVT
                     GIEQREDGLWLVDAEIWVESDSQKRILIGKKGFKVGEIGTGARKDIEGELGGKVHLDL
                     QVKVKRKWRRDEDMLDRLGID"
     gene            complement(977..>1325)
                     /locus_tag="PLV77_09770"
     CDS             complement(977..>1325)
                     /locus_tag="PLV77_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNC16178.1"
                     /translation="GHHVQNMGGISDEVSREKREQSESRNEISVRNELQADCLAGVWA
                     STVFDQLEAGDLDEAIGASEAVGDDRLQSRGGGSVNPDSFTHGTSAQRHRWFKTGFES
                     GDPASCDTFSGDI"
BASE COUNT          234 a          430 c          450 g          211 t
ORIGIN      
        1 atctgcccgg ggtccagcgg ccccgggacg agttgaccag ccgcatgcag aagacggtcg
       61 aaagcgaact ggcgggggcc gatgtcgccc cgctggtgat caatggcgag gaaggcgtcg
      121 ggccgggcga ccggttcatc gccgaaaccc tgctcggggc ccgggccaaa gccggcggct
      181 tgccaatcat ctgcgccgtc aacaaggccg accggctcgg ccggaacatc gtgccggtct
      241 tggtcgccgc cgcggagctc gccggtgtcg acgaggtttt cccggtcagt gccctgaccg
      301 gtcagggcgt gccggaactc gcctcgtacc tggcctcgat cgtccccgag gggccttacc
      361 tctacccgcc ggaggatcac tccgatcagc cgcgggaact gcttctggcc gaactgatcc
      421 ggggccaggc cctgatccgc acccgggacg agattcccca ttcggttgag gtgcgggtga
      481 ccgggatcga gcagcgcgaa gacggcctct ggctggtcga cgccgagatc tgggttgaat
      541 cggactccca gaagcggatc ctgatcggca agaagggctt caaggtcgga gagatcggca
      601 ccggggcccg caaggacatc gaaggggaac tcggcggcaa ggtccacctc gacctccagg
      661 tcaaggtcaa gcgaaagtgg cgccgcgacg aagacatgct ggaccgcctc ggcattgatt
      721 agcccgggtg gcgccaggaa ccaccagccg ctgaagtggg gtggtgttgg cttttcgaga
      781 tcccgttttc tgccatatag cgcacccaac tccgatctcg atcgatatcc ggccggatcc
      841 gggataggcg ccgcaaaaat aggcccatca agggcctgaa tgtgcggcac ctcccgaaac
      901 cagagcctga acccggccct ggaggtacgg cggggaagct cccccacccc gcggcgtaac
      961 caccgggcga atcaggctag atgtcgcccg agaacgtgtc gcagctggcc ggatctcccg
     1021 actcgaagcc ggtcttgaac catctgtggc gctgggcgga ggttccgtgg gtgaacgagt
     1081 cggggttgac gctgccgccg ccgcgggact ggaggcggtc gtcaccgacc gcttcggagg
     1141 cgccgatcgc ctcgtccagg tcgccggcct cgagctggtc gaagacggtt gacgcccaga
     1201 ccccggccag gcagtcggcc tgaagctcgt tacggaccga gatctcgttg cgtgactcgg
     1261 attgctcacg cttctcacgg gacacctcgt cggagatgcc gcccatgttc tggacgtgat
     1321 gccca
//
LOCUS       DAOQMF010000639         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_86206_length_1324_cov_3.812451, whole genome shotgun sequence.
ACCESSION   DAOQMF010000639 DAOQMF010000000
VERSION     DAOQMF010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86206_length_1324_cov_3.812451"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLV77_09775"
     CDS             complement(<1..411)
                     /locus_tag="PLV77_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC16179.1"
                     /translation="MKTALVTGSGGLIGSQSVIKLVEAGWHVVGVENDMRASFFGPEA
                     STRPVSDRLASTLDEFEPVELDIRDAEGVNRVFADRKVELVVHTAAQPSHDWAARDPQ
                     TDFGVNAIGTLNLLEAARNHAPDAPFIFCSTNKVY"
     gene            513..>1324
                     /locus_tag="PLV77_09780"
     CDS             513..>1324
                     /locus_tag="PLV77_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16180.1"
                     /translation="MSLLALLMVISTALLIGLRAHIGFYLDDWDLVVVRSGASDWLLP
                     HNEHIIVIPAALYEASLSVFGMTAMPIHLVAVALFETSVLLLFFWLRRLIGEPVAVLG
                     CAVVLFLGSSTANLVWPFQVGYFGSFAGGLGALLLLRRNTATDDAKACLLLVVSLLFS
                     SMFLPFVVAAAVQLLYREGGRPSLNSLRKRAWVFLVPVALFVIWWLGWGHLAKSFISV
                     ENALKIPLFNLSALGYSVAALTGLFPLREITMSFVWAIPGLIVAAGFAFGLHR"
BASE COUNT          180 a          447 c          420 g          277 t
ORIGIN      
        1 gtagaccttg ttggtcgagc agaagatgaa gggggcatcc ggcgcgtggt tgcgagccgc
       61 ctcgagcagg ttcagggtgc cgatcgcgtt cacaccgaaa tcggtctggg gatcccgggc
      121 cgcccagtca tgcgagggct gggccgcggt gtggaccacc agttcgacct tccggtccgc
      181 gaagaccctg ttcacgcctt cggcgtcgcg gatgtcgagc tcgaccggct cgaactcgtc
      241 gagagtcgag gcgaggcggt cgctgaccgg gcgggtcgac gcctcgggcc cgaagaagct
      301 ggcccgcatg tcgttctcga cgccgaccac gtgccagccg gcctcgacca gcttgatcac
      361 ggactgggat ccgatcagac cgccggatcc ggttaccagg gccgttttca cgaggccttc
      421 ccggccgcag acatggttcg catgcgaagt aacctaacaa gatgggatcg cagcccaccg
      481 gcgccgtcga ggctcttcgc aggcgcccgt gggtgagcct cctcgcgctg ctcatggtga
      541 tctcgaccgc cctgctgatc gggctgagag cgcacatcgg tttctatctc gacgactggg
      601 atctggtggt cgtccgcagc ggcgccagcg actggctgct gccgcataac gagcacatca
      661 tcgtcatccc ggccgcgctc tatgaggcgt cactttcggt gttcgggatg accgcgatgc
      721 cgattcacct ggtcgctgtc gcgctcttcg agaccagcgt gctgctgctg ttcttctggc
      781 tcaggcgcct gatcggcgag ccggtcgcgg tactcggctg cgcggtcgtc ctcttcctcg
      841 gttcatctac cgcaaacctc gtctggccct tccaggtcgg ctatttcggt tccttcgccg
      901 gtggcctcgg agcgctcctt ctgctgcgcc gcaacaccgc gaccgatgat gcgaaagcct
      961 gtctgctgct ggtcgtctcg ctgctcttct cctcgatgtt ccttcccttc gtcgtggccg
     1021 ccgcggtcca gctgctctac cgggaaggcg gccgcccgag cctgaacagt ctgcgcaagc
     1081 gagcctgggt gttcctcgtg ccagtcgccc tgttcgtgat ctggtggctc ggctggggcc
     1141 acctggccaa gagcttcatc agcgtcgaga acgcgctgaa gatccccctc ttcaacctct
     1201 cggcgctggg atattcggtg gccgccctga ctggcctctt cccgctccgc gagataacca
     1261 tgagcttcgt ctgggcgatt cccgggctga tcgtcgccgc cgggttcgcc tttggccttc
     1321 atcg
//
LOCUS       DAOQMF010000640         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_86492_length_1322_cov_2.889503, whole genome shotgun sequence.
ACCESSION   DAOQMF010000640 DAOQMF010000000
VERSION     DAOQMF010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86492_length_1322_cov_2.889503"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLV77_09785"
     CDS             <1..250
                     /locus_tag="PLV77_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16181.1"
                     /translation="GLTPATPDFAHCAAPGDAAEVTGSGLVTTWPSRIEWKLPVTLDH
                     VLAEKGISFSQYDVEKVAGTDHRAVIVELVMPPVPGGR"
     gene            complement(269..592)
                     /locus_tag="PLV77_09790"
     CDS             complement(269..592)
                     /locus_tag="PLV77_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16182.1"
                     /translation="MNPSTALKSGLVMLALVAVLAVLLAVALPKSFFEDWGWFSGPAA
                     LFLCAFGTARILGLPVGRTLLGVFIAGIPGLIGVVTGVHWLGTLLAVLTFAVWCGYGP
                     EPRRA"
     gene            649..>1322
                     /locus_tag="PLV77_09795"
     CDS             649..>1322
                     /locus_tag="PLV77_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16183.1"
                     /translation="MTRSSGIFRSGDRLRQQVSLLFFFLLIAGLAILIPSHASSADQN
                     PIRSSRLSQKGSSLIVQIRTSQKVNLGRLDRRPDFANGNHRYLCLQMNPRGGTTIVRI
                     CLGGRSRTYRVLGVSRTSEAGRVRSSKVIRAEVKRVGQLKLVATFDPAAAGLPPGNYA
                     WRIADHSGECPPSGPKARPTEECLSYFPPKKRAVYRLRPIRVVGCTGGNGELVRNGPR
                     GGKRVAL"
BASE COUNT          233 a          437 c          462 g          190 t
ORIGIN      
        1 cggcctgaca ccggcgacgc ctgacttcgc gcattgcgcg gcgccgggcg acgccgccga
       61 ggtgaccggc agcggcctgg ttacgacctg gccgtcgcgg atcgaatgga aactgcccgt
      121 gaccctcgac catgtgctgg ccgaaaaggg catttcgttc agtcagtacg acgtggagaa
      181 ggtcgcgggc accgatcacc gggccgtgat cgtcgagctg gtcatgcctc cggtgcccgg
      241 cggccggtga gcgccggcac ctgaaccttc aggcgcggcg gggttccggg ccgtagccgc
      301 accagacggc gaaggtgagc acggcgagca gggtgccgag ccagtggact cccgtgacca
      361 cgccgatcag gccggggatg ccggcgatga acaccccgag cagggtgcgg ccaaccggca
      421 ggccgaggat ccgggccgtg ccgaaggcgc agagaaacag tgcggccggg ccggagaacc
      481 agccccagtc ctcgaagaat gacttcggca gcgccacggc gagcagcacc gccagcaccg
      541 ccaccagggc gagcatgacg aggccggact tgagggcggt tgacggattc atgacggcag
      601 acggccgact cgggaaggcc acgccggagc ctatactggg agcgcatcat gacccggtcg
      661 tcaggcatat ttcggtcggg ggacaggctt cgccaacagg tctcactgct gttcttcttc
      721 ctgctcatcg cagggctggc gattctcatc ccttctcacg ccagcagcgc cgaccagaac
      781 ccgattcgct cttccaggct gtcccagaag gggagctcgc tgatcgtcca gatcaggacc
      841 agccagaagg tgaacctggg caggcttgac cgccggcccg acttcgcgaa cggcaaccac
      901 cgctatctct gcctgcagat gaatccccgc ggcgggacca cgatcgtgcg gatctgcctc
      961 ggcgggcgaa gccgcaccta ccgggtgctt ggcgtctcac ggacttcgga ggccggccgg
     1021 gtgcgttcga gcaaggtgat ccgggcagaa gtgaaaaggg tcgggcagct gaaactcgtc
     1081 gccactttcg acccggcggc tgccggtctg cctcccggga actatgcgtg gcggatcgcc
     1141 gaccattccg gcgagtgccc gccgtccggg ccgaaggcga ggccgaccga agaatgcctt
     1201 tcgtacttcc ccccgaaaaa gcgtgcggtc taccggcttc ggccgatccg ggtggtcggc
     1261 tgcacgggtg gcaacggcga gctggtgcga aacggcccgc gcggcggcaa gcgagtggcc
     1321 ct
//
LOCUS       DAOQMF010000641         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_87243_length_1316_cov_3.686757, whole genome shotgun sequence.
ACCESSION   DAOQMF010000641 DAOQMF010000000
VERSION     DAOQMF010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87243_length_1316_cov_3.686757"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1024)
                     /gene="panB"
                     /locus_tag="PLV77_09800"
     CDS             complement(83..1024)
                     /gene="panB"
                     /locus_tag="PLV77_09800"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNC16184.1"
                     /translation="MSSGIRKEGEERRPVTLTRLAEMHEAGEPIAMITAYDFPSAQVI
                     EEAGADIVLVGDSAANCVLGYDSTVPVGMDEMTMLAKAVRRGLTTPFLVGDLPFGSYE
                     GSDELAIASAQRLVKEAGCDAVKLEGGGTSVERAKAIIDAGIPVMGHVGLTPQTATAL
                     GGMKAQGRTAERAIKVAEDAIALDQAGCFAVVIEAVPEAVSEMIVAKMTCPTIGIGAG
                     NATSGQVLVLHDLTGINHGHVAKFVKRYSDVRSEMLKGVEAYIAEVRDGSFPAPEHVY
                     SVDPSELEGLQQRLNREDLEHHSYFESTGSWDWEPLS"
     gene            complement(1021..>1316)
                     /locus_tag="PLV77_09805"
     CDS             complement(1021..>1316)
                     /locus_tag="PLV77_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNC16185.1"
                     /translation="LSPEARERATAIWASLLETESVFSESGLDAALEAGRRHLQSAGI
                     TPEYFEARYPGTLDPAEGTGTDPILVAVAAKVDGTRLIDNILIEPSSTSRRNP"
BASE COUNT          199 a          414 c          463 g          240 t
ORIGIN      
        1 cctggaatcc gggttggtgt agccgcgggg tcgggcgggc atgcccgccc gaccccgcgg
       61 cgttacctcc aacgcatgga gctcaggaga gcggttccca gtcccaggag ccggttgact
      121 cgaagtagga gtggtgttcg aggtcttcgc ggttgaggcg ctgctggagg ccttccagtt
      181 ctgaggggtc gacgctgtag acatgttcgg gggcggggaa ggagccgtcc cggacctcgg
      241 cgatgtaggc ctccacgccc ttcagcatct ccgaacggac atccgaatag cgcttgacga
      301 acttggctac atggccgtga ttgatcccgg tcaggtcatg caggacgagg acctggccgg
      361 aggttgcgtt gccggccccg atcccgatcg tcgggcaggt catcttggcg acgatcatct
      421 ccgacaccgc ctccggcacc gcttcgatga ccaccgcgaa acagccggcc tgatcgagcg
      481 cgatcgcgtc ctcggccacc ttgatcgccc gttcggcggt ccggccctgg gccttcatgc
      541 caccgagggc ggtcgcggtc tgcggggtga gtccgacatg acccatcacc gggattccgg
      601 cgtcgatgat cgccttcgcg cgctcgaccg atgtgccgcc gccttcgagc ttgaccgcat
      661 cgcagccggc ttccttgacc aggcgctggg cgctggcgat ggcaagttcg tccgagccct
      721 cataggagcc gaagggcagg tcgccgacca ggaaaggggt cgtaaggccc cggcgcacgg
      781 ccttggccag catggtcatt tcgtccatgc cgaccggcac ggtcgagtcg tagccgagca
      841 cgcagttcgc ggccgaatca ccgaccagaa cgatgtcggc gcccgcctcc tcgatcacct
      901 gggccgaggg gaagtcatag gcggtgatca tcgcgatcgg ctcgccggcc tcatgcattt
      961 cggccagccg ggtcagggtt acggggcggc gctcttcgcc ttctttgcgg attccggagc
     1021 tcatgggttc ctcctggagg tgcttgatgg ttcgatcagg atgttgtcga tcaggcgggt
     1081 gccgtccacc ttggcggcga cggcgacgag gatcgggtcg gtgcccgttc cttccgccgg
     1141 atcgagggtg cccggatagc gggcctcgaa gtattcgggg gtgatgccgg ccgactggag
     1201 atgccggcgg ccggcttcga gtgcggcgtc aaggccggac tcggaaaaga ccgattcggt
     1261 ttcgagcagc gaggcccaga tcgcggtggc gcgttcacga gcctccggtg agaggt
//
LOCUS       DAOQMF010000642         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_87550_length_1314_cov_2.923749, whole genome shotgun sequence.
ACCESSION   DAOQMF010000642 DAOQMF010000000
VERSION     DAOQMF010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87550_length_1314_cov_2.923749"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..473)
                     /locus_tag="PLV77_09810"
     CDS             complement(78..473)
                     /locus_tag="PLV77_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC16186.1"
                     /translation="MTWMVRPVTPEEKTVREFVATFNRSELDEFAELLDPEVEIHGMR
                     GLRKGRDEARAWATKKPGGVQQTVLIENCRVAGDRVLLGVKREWRWEEDDELAYTDEL
                     AWLLELRGARVLSWKPFEDPEAARTAFEA"
     gene            complement(522..>1314)
                     /locus_tag="PLV77_09815"
     CDS             complement(522..>1314)
                     /locus_tag="PLV77_09815"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNC16187.1"
                     /translation="RDMSEIAREEIDGVIGRLVDEFMAGDIIEEWDLPGLDSQLHQIW
                     PCAIDVAGLPPESTEPEALKQDLCEDAQKAYDEREAEFGEEIMRYLERTLLMQVIDSR
                     WREHLYDMDYLREGIHLRGFAQIDPLVAYKNEGYTMFQELMNGIWEEFSRLIYNVEIE
                     IEPDQNGSFAPPAEEPDEQTYDYSGGTMEAQPSALAEVAAGATPGPADAGGVPGVVPG
                     GVPNVGGAVDPANSATVVKTEREDIGRNDPCWCGSGKKYKKCHGQ"
BASE COUNT          197 a          456 c          400 g          261 t
ORIGIN      
        1 gccgacaccg gcctcgaccg gacctccaac ccccataccg ggggctaacg agccggcgag
       61 cctcgccccg cccgcggtca ggcttcgaag gcggttcttg ccgcttcggg gtcttcgaag
      121 ggtttccagc tgaggaccct ggcgccccgg agttcgagga gccaggcgag ttcgtcggtg
      181 taggcgagct cgtcgtcttc ctcccagcgc cactcccgtt tcacgcccag cagtacccgg
      241 tccccggcaa cccggcagtt ctcgatcagg accgtctgct gaactccgcc cggtttcttc
      301 gttgcccagg cccgggcctc gtcgcggccc ttgcgcagac ctcgcatgcc gtggatttcg
      361 acctcggggt cgagcagctc ggcgaactca tcgagctccg accggttgaa cgtcgccacg
      421 aactcgcgga ctgtcttttc ttcaggagtt acaggcctca ccatccaggt catacagcga
      481 gaccatcagt tgcctctacc gggcgaaatg acctggatgc atcattgccc gtggcacttc
      541 ttgtacttct tgcccgagcc gcaccagcag ggatcgttgc gcccgatgtc ttcgcgttcg
      601 gtcttgacca cggtcgccga gttcgccgga tccaccgcgc cgcccacgtt cgggacgcca
      661 ccgggaacca cacccggaac cccgccggcg tcggccggac cgggagtcgc gcccgcagcg
      721 acctcggcca gggctgaagg ctgggcttcc atggtgccgc ccgagtagtc gtaggtctgc
      781 tcgtccggct cctcggccgg cggggcgaac gatccgttct gatccggttc gatttcgatc
      841 tcgacgttgt agatcaggcg gctgaactcc tcccagatgc cgttcatcag ctcctggaac
      901 atcgtgtagc cctcgttctt gtaggccacc agcgggtcga tctgggcgaa accgcgcaag
      961 tgaatgcctt cacgcaggta gtccatgtcg tagaggtgct cgcgccagcg ggagtcgatc
     1021 acctgcatca gcagggtccg ctcgaggtag cgcatgatct cttcgccgaa ctcggcttcc
     1081 cgttcgtcgt aggccttctg ggcgtcttca cagaggtcct gcttgagcgc ctcgggttcg
     1141 gtcgattccg ggggcaggcc ggccacgtcg atcgcgcaag gccagatctg gtggagttgc
     1201 gaatcgaggc ctggcaggtc ccattcctcg atgatgtcgc cggccatgaa ctcgtcgacc
     1261 aggcgtccga tcaccccgtc gatctcttcc ctggcgatct ccgacatgtc gcgg
//
LOCUS       DAOQMF010000643         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_87586_length_1314_cov_2.160445, whole genome shotgun sequence.
ACCESSION   DAOQMF010000643 DAOQMF010000000
VERSION     DAOQMF010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87586_length_1314_cov_2.160445"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PLV77_09820"
     CDS             <1..1050
                     /locus_tag="PLV77_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNC16188.1"
                     /translation="KRLTEAGPDDSAIDALLVTNLTNVEYLTGFTGTNGACLVSGERQ
                     VFLTDFRYAERAAGIEGWEVEIVSGQWLEGLASRLRGRTGFEDDHVTVRTANQLREAA
                     AQAELVEAGGQVERLRRVKDADEIERIAAAAAMTDELYGLLIERGMVGQTEAEVDGWL
                     TGRMRDQGAEPSFPPIVASGINGASPHAEPGPREIGPGELVTVDMGVKLDGYCSDCTR
                     TLATGPESRLDPLAREIYEVTLRANEAGLGAVKAGAIGSQVDEVARTVIRDAGYGDNF
                     GHGLGHGVGLEIHEAPRLGPSSKDELMAGEIVTVEPGIYISGATGVRIEDLVVVGENG
                     VDRNLSTVTKALTFV"
BASE COUNT          241 a          401 c          469 g          203 t
ORIGIN      
        1 aaacgcctca ccgaggcggg cccggacgat tcggcgatcg atgccctgct ggtcaccaac
       61 ctcaccaacg tcgagtacct gaccggcttc accggcacca acggggcctg cctcgtctcc
      121 ggcgaacggc aggtcttcct gaccgacttc cgctacgccg aaagggcggc cgggatcgaa
      181 gggtgggaag ttgaaatcgt ctccggccag tggctcgaag ggctcgcttc gaggctccgg
      241 ggccggaccg gcttcgaaga tgatcacgtc acagttcgca ccgcgaatca gttgcgcgaa
      301 gccgcggccc aggcggagct cgtcgaggcc ggcggtcagg tcgagcgcct gcgccgcgtg
      361 aaggacgcgg acgagatcga aaggattgcg gccgccgctg cgatgaccga tgagctttac
      421 gggttgctga tcgagcgcgg gatggtcggc cagaccgagg cagaggtcga cggctggctg
      481 actggccgga tgcgcgacca gggcgccgaa ccttccttcc cgccgatcgt cgcgtcagga
      541 atcaatggcg cctcgccgca tgcggaaccc ggaccccgcg agatcgggcc gggcgagctg
      601 gtcacggtcg acatgggcgt gaagctcgac ggctactgct cggactgcac gaggaccctt
      661 gcgaccggcc cggaatcccg cctcgacccg ctggcccgtg agatttacga ggtgaccctg
      721 agagccaacg aggcgggcct cggcgcggtg aaggccggcg cgatcggatc ccaggttgac
      781 gaggtcgcca gaacggtgat ccgcgacgcc ggctacggcg ataacttcgg gcacggactc
      841 ggccacgggg tcggcctgga gatccacgag gcgccccggc tcggtccgag cagcaaggac
      901 gagctgatgg cgggcgagat cgtcacggtc gaaccgggga tatatatctc tggcgcgacc
      961 ggtgtgagga tcgaggatct ggtcgtggtt ggggagaacg gagtggaccg gaacctgagc
     1021 acggtcacca aggccctgac cttcgtgtag ggggaatgca tcttttgtaa cgatcctcta
     1081 tagtgtgata cccgtcacac agacgacatt gcttctgtca cgagtgggga gggaactcca
     1141 gagcaatatg gcgagggagt ttttgaggag agaggtgcag acgaaaagac cggcctgagg
     1201 gggccggtct tttcattgca tcaacggccg cagccgagac ggagcccggg tgccggccca
     1261 gagccagctc gcctccaccg gggtgggtcg cctccaccgg ggtgggtcgc ctcc
//
LOCUS       DAOQMF010000644         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_87794_length_1312_cov_3.731901, whole genome shotgun sequence.
ACCESSION   DAOQMF010000644 DAOQMF010000000
VERSION     DAOQMF010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87794_length_1312_cov_3.731901"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLV77_09825"
     CDS             complement(<1..212)
                     /locus_tag="PLV77_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16189.1"
                     /translation="MNSGSAAGRSLNGYALARVGLGAVLAACLAVLLLAPDQGPPVPD
                     PAAAARVIADRFSPHELDRAGDYRSWG"
     gene            246..983
                     /locus_tag="PLV77_09830"
     CDS             246..983
                     /locus_tag="PLV77_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC16190.1"
                     /translation="MKVAVISDIHANRHALDAVLADIDESGIEEIWFLGDAVGYGAFP
                     GECVLTLKDRCSVFLLGNHDLAALREIDIGTFSPSAAISARWTREHMDEDAFEVLRGL
                     DGTSAEREGIGLFHASPRDPIWEYVVDSDLAEDNLDFQEQRVALIGHSHIALYFTRVD
                     EMSRVSSVLAPEGTELEMSAGKWLLNPGSAGQPRDGDPRSAWAELDTESFQARFHRVQ
                     YPVEEAAEAIRKAGLPSHLGDRLFKGQ"
     gene            1038..>1312
                     /locus_tag="PLV77_09835"
     CDS             1038..>1312
                     /locus_tag="PLV77_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16191.1"
                     /translation="MPFFARHRPRRGFLPALFASLALSLLLAGCGEDRSNLIPEETSN
                     SLISKINRAEALAADHKCFAAGDVAASAQAEIEGLGGKIDPELKRSL"
BASE COUNT          241 a          429 c          417 g          225 t
ORIGIN      
        1 ccccaggaac ggtagtcgcc ggcccggtcc agttcatgcg ggctgaaccg gtcggcgatc
       61 acccgggctg cggcggcagg atcgggaacc ggaggcccct gatcgggagc gagcagaagc
      121 accgccagac aggccgccag caccgcgccc aacccgaccc tggccagggc gtagccgttc
      181 aatgaccgac ccgcggccga acccgaattc attgctcaat acgatattcg gcgggccgcc
      241 tgaccatgaa agtcgccgtc atctcggaca tccacgccaa ccggcacgca ctggacgccg
      301 tgctcgccga catcgatgaa agcgggatcg aggagatctg gtttcttggg gatgcggttg
      361 gctacggcgc ctttcccggc gaatgcgtac tgaccctgaa ggatcgctgt tcggtcttcc
      421 tgctcggaaa tcacgacctg gccgcgctcc gggagatcga catcggcact ttcagcccct
      481 cggctgcgat atctgcccgc tggacccggg aacacatgga cgaggacgcc ttcgaggttc
      541 tgcgtggcct ggatggaacc tcggccgagc gggaggggat cggccttttt cacgcctcgc
      601 cgcgagatcc gatctgggaa tacgtcgttg acagcgatct ggccgaagac aatctcgact
      661 tccaggaaca gcgggtcgcc ctgatcggcc attcccacat cgccctctac ttcacccggg
      721 ttgacgagat gtccagggtt tcgtccgtgc tggcgccgga aggcaccgag ctcgagatga
      781 gcgccgggaa gtggctgctc aacccgggca gcgccgggca gccgcgggac ggcgacccac
      841 ggtcagcctg ggcggaactg gacacggaga gcttccaggc ccgtttccac cgggttcagt
      901 acccggtcga agaggccgca gaagcgatcc gaaaggccgg tcttccctcc catctgggtg
      961 accggctctt taagggccag taaaggtccg ggatgcgtca caaaggccag cggtccaacc
     1021 atctacactc ggcgggaatg ccgttttttg cccgacaccg cccccgccgg ggttttctgc
     1081 ctgccctgtt tgccagcctg gccctctccc tgctcctggc cggatgtggc gaggatcgga
     1141 gtaacctgat acctgaggaa acgtcgaatt cgctgatctc caagatcaac cgtgccgagg
     1201 cgctggccgc cgatcacaag tgttttgcgg ccggtgacgt ggcggcttcg gcccaggccg
     1261 agatcgaagg actcggcggc aaaatcgacc cggagctgaa acgttcactg at
//
LOCUS       DAOQMF010000645         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_87844_length_1312_cov_2.591885, whole genome shotgun sequence.
ACCESSION   DAOQMF010000645 DAOQMF010000000
VERSION     DAOQMF010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87844_length_1312_cov_2.591885"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLV77_09840"
     CDS             complement(<1..261)
                     /locus_tag="PLV77_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16192.1"
                     /translation="MFVFLGVTGPAQAAAPASILDGQIPCGVVTDEGSGGSAVSTSLG
                     QVWCGTIRPSDNTGATVTPAIESVRSTAKTTDGVPLDINFAMP"
     gene            complement(388..>1312)
                     /locus_tag="PLV77_09845"
     CDS             complement(388..>1312)
                     /locus_tag="PLV77_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16193.1"
                     /translation="GIFNPAPTGQACSTAPGGKETGTASYELDPAPAGGFTVLGAPTV
                     VAKIQQEGVNSQIAARLVDVSPDGTTKLLVARGLWRPQGEGFQVFQLHANAWKVEEGH
                     VLRLELLPKDASVPAGLVANYGRPSNGQTDATVTDLDLRIPVADQPGAAGGLVTAPAA
                     KVLPDRPGVKLAPGYDTDDQVPIDDYEPPPVDHPKTIRKRIVIGKVQIKGKRVLIKAG
                     CPKAATVCPKARITVAGAKRKTRGTLARIKGVVVKPGEDRLVSTRLTKRGQKVLKQTR
                     GRKLAVKVTVKPAGMSKRTVRRVARVTRQRR"
BASE COUNT          176 a          442 c          452 g          242 t
ORIGIN      
        1 gggcattgcg aagttgatgt ccagcgggac gccgtcggtg gtcttcgcgg tgctgcggac
       61 cgattcgatt gctggggtga cggttgcgcc ggtgttgtcg gaggggcgga tcgtgccgca
      121 ccagacctgt cccagggagg tgctgacggc gctgccgcct gaaccttcat cggtcacgac
      181 gccacaggga atctgcccgt ccaggatgct cgcgggtgcc gccgcctgcg cgggtccggt
      241 cacgccaagg aacacgaaca ctgcaagaac aacggctctc cgtaccactt caacccctcc
      301 cggtcgtact cccggccact tgccgaaccg cggggatcgt agcgctgatc aaagatatat
      361 gcaagcccgc agatttccgc gggggcccta cctgcgctgg cgggtgaccc tcgctacccg
      421 tcgcaccgtt ctcttcgaca tcccggccgg tttgacggtg accttcaccg ccagcttccg
      481 gcccctggtc tgtttcagca ccttctgccc gcgtttggtc aggcgggtcg aaaccagccg
      541 gtcttcgccg ggtttgacga ccactccctt gatcctggcc agtgtcccgc gggtcttccg
      601 ctttgcgccg gccacggtga tccgcgcctt cgggcagacg gttgctgcct tcgggcaacc
      661 ggccttgatc agcactcgct tgcccttgat ctgaaccttg ccgatcacga tccgtttcct
      721 gatcgtcttc gggtggtcga ccggcggcgg ctcgtaatcg tcgatcggaa cctggtcgtc
      781 ggtgtcgtag ccgggggcaa gcttcacgcc gggccggtcg ggcaggacct tggcggccgg
      841 cgcggtgacc agaccgccgg ccgcgccggg ctggtcggcg accgggatcc gcaggtcgag
      901 gtcggtgaca gtggcatcgg tctggccgtt cgaggggcgg ccgtagttcg cgacgaggcc
      961 ggccggcacc gaggcgtcct tcggcagcag ttcgagtcgc agcacgtggc cctcctcgac
     1021 cttccaggcg ttggcgtgta gctggaagac ctggaaaccc tcaccctgcg ggcgccagag
     1081 gccacgggcg accagcagct tggtcgtccc gtcgggcgag acgtcaacca gccgggccgc
     1141 gatctgcgag ttgacgcctt cctgctggat cttcgcgacc acggtcggag cgccgagtac
     1201 ggtgaagccg ccggccgggg ccgggtcgag ctcgtagctg gccgtcccgg tttccttgcc
     1261 gccgggcgcg gtcgagcagg cctggccggt cggggcggga ttgaagatcc cg
//
LOCUS       DAOQMF010000646         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_88689_length_1305_cov_4.763200, whole genome shotgun sequence.
ACCESSION   DAOQMF010000646 DAOQMF010000000
VERSION     DAOQMF010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88689_length_1305_cov_4.763200"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLV77_09850"
     CDS             complement(<1..615)
                     /locus_tag="PLV77_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNC16194.1"
                     /translation="MTENGLNGNGNGASRGGDDKVRVAIVGVGNCANSFIQGVQYYKD
                     ADPADQVPGLMHVDLGGYHVKDIEFVAAFDIDQEKVGKDLSEAIWSGQNDTIKFADVP
                     NLGVTVERGMTLDGLGKYLKQVITKAPGPTADIVQILKDTKTDVLVCYLPVGSEDAVK
                     WYVEQAIAAGVGFVNCLPVFIAREDYWDKRFQEAGLPIVGDDIKS"
     gene            complement(796..>1305)
                     /locus_tag="PLV77_09855"
     CDS             complement(796..>1305)
                     /locus_tag="PLV77_09855"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNC16195.1"
                     /translation="LIAAGLVLHGDFFFAGIAFIIGSVMDTLDGRYSRMSGKGTLFGA
                     FLDSTLDRIEEGIVLAAVAWQFADSGDSVAAAMCVVVVLGSLMVSYTRARAEALGVEC
                     KVGIATRPVRVVILSAGLVLGGEEVISGFDLLPIAIYVMAGLTAITTLQRVWHVRTEL
                     KKLDAAATE"
BASE COUNT          241 a          414 c          423 g          227 t
ORIGIN      
        1 cgacttgatg tcgtcgccga cgatcggcag accggcctcc tggaaacgct tgtcccagta
       61 gtcctcgcgg gcgatgaaca ccggcaggca gttgacgaag ccgacgccgg cggcgatcgc
      121 ctgctcgacg taccacttga cggcgtcttc ggagccgacc ggcaggtagc agaccagaac
      181 gtcggtcttg gtgtccttga ggatctggac gatgtcggcg gtcgggccgg gggccttggt
      241 gatgacctgc ttgaggtact tgccgaggcc gtcgagggtc atgccgcgct cgaccgtgac
      301 gccgaggttg ggaacgtcgg cgaacttgat cgtgtcgttc tggcccgacc agatcgcttc
      361 ggaaaggtcc ttgccgacct tctcctgatc gatgtcgaag gcagccacga actcgatgtc
      421 cttgacgtgg tagccgccga ggtcgacatg cataagtccg ggaacctgat ccgccggatc
      481 ggcgtccttg tagtactgga ctccctggat gaaggagttg gcgcagttgc cgacgccgac
      541 aatggcaact cgtaccttgt cgtcaccgcc acggctggcg ccgttgccgt ttccgttaag
      601 tccgttctca gtcatgggta agcgaaagtc tcctgtgggt ggggtggcat ctcttgccgg
      661 ttgggcaccg gtccgcacgc tgatcagggg caaaaagcca cgcctgatcc gggcgcgggg
      721 ccgggtagac ccctaatgac accacaaaac ggccccggtt acaggctgcc gggcattaat
      781 ttcgggcctg tccgcctatt cagtcgcggc cgcgtcgagc ttcttcagct cggtccggac
      841 atgccagacc cgctggaggg tggtgatcgc ggtcaatccc gccatcacgt aaatggcgat
      901 cggcagcagg tcaaagcccg agatcacttc ctccccgccg aggaccagcc cggccgaaag
      961 gatcacgacc cgaaccggcc gggtggcgat accgaccttg cattcgaccc cgagcgcctc
     1021 ggcccgggcc cgggtgtagc tgaccatcag cgatccgagc acgaccacca cgcacatcgc
     1081 ggcggccacg ctgtcaccac tgtcggcgaa ctgccaggcg accgcggcga gcacgatgcc
     1141 ctcttcgatc cggtcgaggg tggaatcgag gaaggcgccg aacagcgtgc ccttgcccga
     1201 catccgcgag taacggccgt cgagggtgtc catcaccgaa ccgatgatga aagcgatgcc
     1261 ggcgaagaag aaatcgccgt gaagcacgag accggcggcg atcag
//
LOCUS       DAOQMF010000647         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_88862_length_1304_cov_2.977582, whole genome shotgun sequence.
ACCESSION   DAOQMF010000647 DAOQMF010000000
VERSION     DAOQMF010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88862_length_1304_cov_2.977582"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLV77_09860"
     CDS             complement(<1..647)
                     /locus_tag="PLV77_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16196.1"
                     /translation="MLIGNQTKARLVATLAGALVALFSLVAVARAIVEQEPNDIRAEA
                     NGPIMDGDTWSGAFGAGGEFGEADWLKFYIAKPGTEVSFDITRGVVLENAANFELTTA
                     SGDSIEGGAAATIPDGGTTGNITKTLSPGKYHFWVVGITDGPDFGMTWQIEAHGDFAT
                     FADIQAACSSGKAKLPNLSQAVASAKKALNKAKKRKQGKAAAVKKAKARLAKAQKA"
     gene            786..>1304
                     /locus_tag="PLV77_09865"
     CDS             786..>1304
                     /locus_tag="PLV77_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16197.1"
                     /translation="MTDPAPQQTPDESAKRDRIPRLAIAVVVVGLLATAAVYAIAQLP
                     GSGSLEIDWDTTEVVSTPPARDLGNGSFAVARSSLSALAPNDEGGLIYRVAGVVRIDS
                     GGRKLTTVRCDVNSNAAGETNRMAHSSRLRAAWPKPSDALQEQEVPETSFVKFRTPDS
                     KKIDLPIRDVVQR"
BASE COUNT          215 a          458 c          382 g          249 t
ORIGIN      
        1 gccttctggg ccttggccag tctcgccttc gccttcttta ccgcagcggc cttgccctgc
       61 ttgcgtttct tcgccttgtt cagggccttc ttcgcgctgg ctacggcctg gctgaggttc
      121 ggcagcttcg ctttgcccga actgcacgcc gcctggatgt cggcaaaggt cgcgaagtcg
      181 ccgtgcgcct cgatctgcca tgtcatcccg aaatcgggtc cgtccgttat gccgaccacc
      241 cagaagtggt acttgcccgg actcagggtc ttggtgatgt tgccggtagt gccgccgtcc
      301 ggaatggtcg ccgcggcgcc gccctcgatc gagtcaccac tcgcggtcgt gagctcgaaa
      361 ttcgctgcgt tctccagcac gaccccgcgc gtgatgtcga agctcacctc cgtccccggt
      421 tttgcgatgt agaacttcag ccagtcggct tctccgaact cgccacccgc gccaaaggca
      481 ccggaccagg tgtcaccgtc catgatcggt ccgtttgctt cggctcgaat gtcgttcggc
      541 tcctgctcga cgatcgccct ggcgacggcg acaagactga agagtgcgac aagagcgcca
      601 gcgagcgttg cgacaagccg cgccttcgtc tgatttccga tgagcatttg atcccccaaa
      661 tcccggtttc agctcgacat tcccacccta accgggttat gtaaagccgc gcaacccttg
      721 aggccggaac cgtcccgacg cgctgccggc cgcgccgcat cgaatatcct ccgcaccgcc
      781 gtccgatgac tgacccagcc cctcaacaga cacccgacga gtccgcgaag cgtgaccgca
      841 ttcctcgcct ggcgatcgcc gtggtcgtgg tcggcctgct ggccacggcc gccgtctacg
      901 cgatcgcgca gctgcccggc agcggctcgc ttgaaatcga ctgggatacc accgaggtgg
      961 tcagcacacc acccgccagg gatctcggca acggctcgtt cgcggtggcc agaagcagcc
     1021 tttccgcgct ggcgcccaat gacgagggcg gactcatcta ccgggtggcc ggcgtggtcc
     1081 ggatcgattc cggcggacgc aaactgacca cggtccgctg cgacgtcaat tcgaacgctg
     1141 ccggcgaaac gaaccggatg gctcactcga gccgcctgcg ggccgcctgg ccgaagccca
     1201 gcgacgccct tcaggagcag gaagttccgg aaacgagttt cgtcaagttc cgcacccctg
     1261 actcaaagaa gatcgacctg ccgattcgcg atgtcgttca gagg
//
LOCUS       DAOQMF010000648         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_88907_length_1304_cov_2.342674, whole genome shotgun sequence.
ACCESSION   DAOQMF010000648 DAOQMF010000000
VERSION     DAOQMF010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88907_length_1304_cov_2.342674"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLV77_09870"
     CDS             <1..432
                     /locus_tag="PLV77_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16198.1"
                     /translation="TEGPDSSRIAADDRVMRIDLHPGINLESMADMLIRDGFRGAVMT
                     LYPALTGPFEPERSSVPRFAHRLAGHGIVLALTMNAQPVGVQNSYESKLLLEETGAVL
                     LPVIPELALVKLMWALGWSSDPDEVRQVLAEEICNEFGGRK"
     gene            545..>1304
                     /locus_tag="PLV77_09875"
     CDS             545..>1304
                     /locus_tag="PLV77_09875"
                     /inference="COORDINATES: protein motif:HMM:NF028011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HNC16199.1"
                     /translation="MPGDERISLNEAARRAGVSPATLTRWADEKIIPVRKGRWTPAAA
                     AQARIVARMRERGHSLEELKEAGKEGKLAFGMAEDIFADPTPQVDLETAAKEIGLSVE
                     VIERVMTVMGTPSDREQSLSARDIEAFKRMGEVAEAGLPVEALLQLTRVYAQAVRRIA
                     DAEVRLFHLFVHEPMIRDGLSEIEMAEELGDLARRTSPTAVPLFQYLHDRYLRYFLEQ
                     DVIGHMESDFGGDTNLDQVRTTLCFIDLTGFTRFT"
BASE COUNT          241 a          432 c          415 g          216 t
ORIGIN      
        1 accgagggtc cagattcttc ccggatcgcc gccgacgacc gcgtgatgag aatcgatctc
       61 cacccgggga tcaatctcga atcgatggcc gacatgttga tccgcgacgg cttccgcggc
      121 gcggtgatga ccctttaccc ggctctgacc gggcctttcg aaccggaacg ctcctcggtg
      181 ccgcggtttg cccatcggct tgccgggcac gggatcgtgc tcgccctgac catgaacgcc
      241 cagccggtcg gggtccagaa ttcctacgaa tccaaacttc tgctcgagga gaccggggcc
      301 gtgcttctgc cggtgatccc cgagctcgcc ctggtcaagc tgatgtgggc tctcggctgg
      361 agcagtgacc cggacgaggt tcgccaggtc ctcgccgaag agatctgcaa cgagttcggc
      421 ggaagaaagt agctgcaagt cgggcggggt ccgacccgcc ctccaagttg aacccgtccg
      481 atcacagaag aaattgatgg ttgatcggac tcagtcgggt gactctccca ataccatcta
      541 gccgatgccc ggggatgagc gaatcagcct taatgaggcg gcccggcggg ccggcgtttc
      601 accggcgacc ctgacccgct gggcggacga gaagatcatc ccggtccgga agggccgctg
      661 gaccccggcc gctgccgccc aggcccggat cgtcgcccgg atgcgggaga gagggcattc
      721 cctcgaggaa ctcaaggagg ccggcaagga aggcaagctc gccttcggca tggccgagga
      781 catcttcgcc gaccccaccc cccaggtcga cctggaaacg gccgcgaagg agatcgggct
      841 cagcgtcgag gtgatcgaac gggtgatgac agtcatgggc actccgtccg accgcgagca
      901 gagcctctcc gccagagata tcgaagcgtt caagcgaatg ggggaggtgg ccgaggccgg
      961 tctgccggtc gaagccctgc tccagctgac cagggtgtac gcccaggccg tacgacggat
     1021 cgccgacgcc gaggtccggc tcttccatct cttcgtccat gagccgatga tccgtgacgg
     1081 cctgagcgag atcgaaatgg ccgaggaact cggcgacctg gcccgccgca cctcgcccac
     1141 cgcggtgccg ctcttccagt acctgcatga ccggtatctc aggtacttcc tcgaacagga
     1201 cgtgatcggg cacatggagt ccgacttcgg tggcgacacc aacctcgacc aggtcaggac
     1261 cacgctctgc ttcatcgacc tgaccgggtt cacccggttc accg
//
LOCUS       DAOQMF010000649         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_89127_length_1302_cov_3.132318, whole genome shotgun sequence.
ACCESSION   DAOQMF010000649 DAOQMF010000000
VERSION     DAOQMF010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89127_length_1302_cov_3.132318"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1302
                     /locus_tag="PLV77_09880"
     CDS             <1..>1302
                     /locus_tag="PLV77_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16200.1"
                     /translation="ITDVNQETGTVVLEDAYGNRYSYAGLGSVSTVHPVPQPEPVSSL
                     VPEESDSGSAPAGDASAGDGSSGQPDPEAEMGEEKAAAPTPAEKEAFGSNPQAEDQGS
                     AEPTKQQQQDISEVLVDPDAAQAAAAAAATDPATSDDSNPAVKAADDRRAEQDSIRQV
                     PAEADRNTEDSRGRVYASPLRPSNQHRATTDGQSIQNEIRQAEGVGIEGKPGDYLVYD
                     GSKSGIYRFDPETTELRELKKGSRVIAGTVLGRLAETPVASVNFSIKPGGEDTPQIDP
                     KPFLDGWKLLGETNIYSARGKDRFSQRLGVGGILLLSKSALQKRVLNDPKMAFTQQCD
                     RDYIAAGSIDRRLLATMEFITAHGYSLTISSMLCGRETSITTSGNISNHTHGGAMDIA
                     IINGEVVTPGSQGPGSLTDQVARLVLTLQGTMAPDEVISLM"
BASE COUNT          257 a          483 c          387 g          175 t
ORIGIN      
        1 gatcaccgac gtcaaccagg aaaccggcac ggtcgtgctc gaggacgcct acggcaaccg
       61 gtactcctac gccggcctcg gttcggtttc aaccgtccac ccggtacccc agcccgaacc
      121 ggtctcctcg ctggttccgg aagaatctga ttcgggatcg gccccggccg gagatgcctc
      181 cgccggtgac ggctcttccg ggcagcccga ccccgaagcg gagatgggcg aggaaaaggc
      241 agccgccccc accccggccg aaaaggaagc cttcggatct aaccctcagg ctgaggatca
      301 gggttcggcc gaaccgacca aacagcagca gcaggacatc agcgaggtgc tggtcgatcc
      361 cgacgccgcc caggcggccg ccgccgcagc cgcgaccgac cccgcgacga gcgacgattc
      421 caaccccgcg gtcaaagccg ccgacgaccg gcgtgccgag caggacagca tccgccaggt
      481 gcccgccgag gcagaccgca ataccgagga cagccgcggc cgcgtctacg ccagcccgct
      541 tcgtcccagc aaccagcacc gcgcgacgac cgacggccag tcgatccaga acgagatccg
      601 ccaggccgaa ggcgtcggaa tcgagggcaa gcccggcgac tacctggtct atgacggcag
      661 caagtccggc atctaccgct tcgaccctga aaccaccgaa cttcgggaac tgaagaaggg
      721 ctcgcgggtg atcgccggca ccgtgctcgg ccgcctggcc gaaacgccgg tcgcctcggt
      781 caacttctcg atcaagcccg gcggcgaaga caccccgcag atcgacccca agccgttcct
      841 cgacggctgg aagctgctcg gcgaaaccaa tatctacagc gcccgcggca aggaccgctt
      901 ctcgcagcgg ctcggtgtcg gcggcatcct cctgctttcg aagtcggccc tccagaagcg
      961 ggtgctgaac gacccgaaga tggccttcac ccagcaatgc gaccgcgatt acattgctgc
     1021 cggctcgatc gaccgccgcc tgctggcgac catggaattc atcaccgccc acggctactc
     1081 gctgaccatc agctcgatgc tctgtggccg cgagacctcg atcaccacct ccggcaacat
     1141 ttccaaccac acccacggtg gcgcgatgga catcgcgatc atcaacggcg aggtcgtcac
     1201 tccgggcagc cagggccccg gttcactcac cgaccaggtg gcccgtctgg ttctgaccct
     1261 gcagggcacc atggccccgg acgaggtcat ctcgctgatg ga
//
LOCUS       DAOQMF010000650         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_89806_length_1297_cov_4.034622, whole genome shotgun sequence.
ACCESSION   DAOQMF010000650 DAOQMF010000000
VERSION     DAOQMF010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89806_length_1297_cov_4.034622"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..>1297)
                     /locus_tag="PLV77_09885"
     CDS             complement(93..>1297)
                     /locus_tag="PLV77_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16201.1"
                     /translation="LQRSIARAFTYQLLPSVNAQATMGTPKHVLRYLIDHLGDGSQQS
                     WNLLIFQWGFQAGHAITPYAIEDMGGGIYEIHVYDNNWPNDDTRRLVVNTNRNTWSYY
                     ASTQPGIPAAEYRGNARSGTLFLRPNTPALGIQPCPYCIGRQGANSKYNQVTLSYTAD
                     QHARLLITDSKGRQTGFKDGKPINRIPGAKVIRQATSPITFAADGAIENIADDPEPVY
                     LIPKNLKLRIRIDGRHMTVTDRESLGVVGPTFDSTVENLKMGPGKVAFATLSPKAKTL
                     SITGARGESSPRVTFGAQSRKAAYRVKVSAIGAAPQSTFYFAKKPNYGLLRIGKKATG
                     PQAWKVAINKFDARGNQTRFVRSYVLRGNQIAFLYYGPLAVGKRAYVVIASPNGNKVK
                     LLKLKRSQ"
BASE COUNT          198 a          375 c          457 g          267 t
ORIGIN      
        1 gtcactaact gcagtcgcgc gggtctttgc ccgcgctccc atctcaaccc gcccctccca
       61 ctcgaagaaa aggatggttg ggaggggctc tgttattgac tcctcttgag cttcaggagc
      121 ttgactttgt tgccgttggg ggaggcgatc acgacatagg cgcgcttgcc gactgcgagc
      181 ggcccgtagt agaggaaggc gatctggttg ccgcgcagga cgtagctgcg gacgaatcgg
      241 gtctggttgc cacgggcgtc gaacttgttg atcgccacct tccaggcctg gggcccggtc
      301 gccttcttgc cgatcctgag cagaccgtaa ttgggcttct tggcgaaata gaaggtcgac
      361 tgcggggccg cgccgatcgc actcaccttg acccggtagg cggccttcct gctctgggcc
      421 ccgaaggtga cccgcggcga ggattcaccc ctggccccgg tgatcgaaag ggtcttggcc
      481 tttggcgaaa gggtcgcgaa ggcgaccttg cccggaccca tcttcaggtt ctcgacggtc
      541 gagtcgaagg tcgggccgac cacccccagc gattcgcggt cggtgaccgt catgtgcctg
      601 ccgtcgatcc ggatccggag cttcaggttc ttggggatga ggtagaccgg ttcgggatcg
      661 tcggcgatgt tttcgatcgc gccgtcggcg gcaaaggtga tcggcgaggt cgcctgccgg
      721 atgaccttgg caccggggat ccggttgatc ggcttgccat ccttgaaccc ggtctggcgc
      781 cccttcgaat cggtgatcag cagccgtgcg tgctggtcag ccgtgtagga gagggtgacc
      841 tggttgtact tcgagttggc tccctggcgg ccgatgcagt acgggcaggg ctggatcccg
      901 agggcgggcg tgttcgggcg gaggaaaagg gtccctgagc gggcgttgcc cctgtactcg
      961 gcggcgggga tgccgggctg ggtggacgcg tagtagctcc atgtgttccg gttcgtgttg
     1021 acgaccagcc tgcgggtgtc gtcattcggc cagttgttgt catagacgtg aatctcgtag
     1081 atgccgcccc ccatgtcctc gatcgcgtat ggggtgatcg cgtgaccggc ctggaagccc
     1141 cactggaaga tcagcaggtt ccaggactgc tgagagccgt ccccgaggtg gtcgatcagg
     1201 taccggagca cgtgcttcgg agtgcccatc gtcgcctgcg cgttgaccga gggaagcagc
     1261 tggtaggtga aggcccgggc gattgaccgc tggagac
//
LOCUS       DAOQMF010000651         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_90653_length_1291_cov_3.415858, whole genome shotgun sequence.
ACCESSION   DAOQMF010000651 DAOQMF010000000
VERSION     DAOQMF010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90653_length_1291_cov_3.415858"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PLV77_09890"
     CDS             <1..948
                     /locus_tag="PLV77_09890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNC16202.1"
                     /translation="MKSTVPAGTGASIRRELPGLAYVSCPEFLKEGAAVEDFMHPDRV
                     VVGSEPEDSAAAEEVADLYRPLGGEFVMTDTSSAEMIKLASNAYLATRISFINEIANV
                     CEEVGADVSEVARGMGLDERIGPHFLRPGIGFGGSCFPKDVNALKLLAGNSGYHFQLL
                     NSVIEVNELQKRRVVSKLTKHLGPLVGKRIALLGLAFKPDTDDMREASSLVLSARLRG
                     EGAEVVAYDPVAMSNARSLLPGVEMSDTAQEALAGSDAAVLVTEWSEFKEIDWTEAAD
                     RMRRPLLIDGRNFLDAGQLRSAGFLYEGIGKAGTGDESG"
     gene            955..>1291
                     /locus_tag="PLV77_09895"
     CDS             955..>1291
                     /locus_tag="PLV77_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNC16203.1"
                     /translation="MQALVLVGGKGTRLRPLTYETPKPALTLVDRPFLNYMTEWLGSH
                     GVDEVILACGFLPDELKKVLGEGEPGGPKLRYLVEEETLGTAGAIRFALDHLDERIAA
                     LGVAEHHRSA"
BASE COUNT          261 a          382 c          423 g          225 t
ORIGIN      
        1 atgaagagca cggtgccggc cggcaccggg gcctcgatcc ggcgcgagct tccggggctg
       61 gcctacgtgt cctgcccgga gttcctgaag gaaggcgcgg cggtcgagga cttcatgcac
      121 cctgaccggg tcgtggtcgg atccgagccg gaggacagcg ccgcagcgga agaagtcgcc
      181 gacctctacc gcccgcttgg tggcgagttc gtgatgaccg acacctcgag cgccgagatg
      241 atcaagctcg cctcgaatgc ctacctggcg acccgcatct ccttcatcaa cgagatcgcg
      301 aacgtctgcg aagaggtcgg tgccgacgtc agcgaggtcg cacgggggat gggtctggac
      361 gagcggatcg gcccacattt cctgcgtccc gggatcggct tcggcggttc ctgctttccc
      421 aaggacgtca atgccctgaa attactggcc ggtaactcgg gctaccactt ccagctgctc
      481 aactcggtga tcgaagtcaa cgaactccag aaacgacggg tcgtgagcaa actgacgaaa
      541 cacctggggc cgctggtcgg caagcggatc gcccttctgg gcctcgcgtt caaacccgac
      601 accgacgaca tgagggaagc atcgagcctc gttttgtccg ctcgcctgag aggcgaaggt
      661 gcggaagttg ttgcatatga ccccgtagcg atgagtaatg caaggagtct cctgccgggg
      721 gtcgaaatga gtgacacagc ccaggaggca cttgcaggtt cggacgccgc ggtactggtt
      781 accgagtggt cggaattcaa ggagatcgat tggactgagg cggccgatcg gatgcggagg
      841 ccattgctca tagacggacg aaacttcctt gatgccggcc aactccgctc cgccgggttc
      901 ctttacgaag gaatcggcaa ggctgggacc ggtgacgaaa gcgggtaaac gaaattgcag
      961 gcattggtac tggttggcgg taagggaacc cgactccgac ccctgactta cgagacgccg
     1021 aagccggccc tgaccctggt cgaccggccg tttctgaact acatgaccga gtggctgggc
     1081 agccacgggg tcgacgaagt catccttgcc tgcggttttc tgccagatga attgaagaag
     1141 gtcctcggtg aaggagaacc gggcgggccg aagctcaggt atctggtcga ggaggaaacc
     1201 ctcggcaccg caggggcgat ccgtttcgcc ctcgaccacc ttgacgagcg aatcgccgcc
     1261 cttggagtcg cggaacacca ccgaagcgcg c
//
LOCUS       DAOQMF010000652         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_90694_length_1291_cov_2.733010, whole genome shotgun sequence.
ACCESSION   DAOQMF010000652 DAOQMF010000000
VERSION     DAOQMF010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90694_length_1291_cov_2.733010"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="PLV77_09900"
     CDS             complement(<1..125)
                     /locus_tag="PLV77_09900"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC16204.1"
                     /translation="MSFYVTTPIFYGNGEPHLGHAYSTMAADILARHMRQRGEEV"
     gene            complement(163..1074)
                     /locus_tag="PLV77_09905"
     CDS             complement(163..1074)
                     /locus_tag="PLV77_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16205.1"
                     /translation="MSAVGSRVAYATCEDRISDWEDDLLSADLLAERGVEVEFAVWND
                     PAVDWGSFDLTVIRSTWDYTERLEEFLAWCESVGPGKLRNVPAMILWNTDKRYLAELD
                     GAGLPVPPTALVAPGSNPPPFGGKVVIKPVSGAGARDTGVFDEANEEGGLALLEKLAR
                     QDEIAMIQPYIPWIDERGETAVLFFGGRFAYALKKRAFLPESGVAPVRPGTTVAEGMF
                     AEDLMSLADASEAEVELGAKVVAWLARRFGSVPLYARIDMVSSPQGEPVLMEVEVTEP
                     SFYLGLTTGLETPGPELFAAAVEAGLG"
     gene            complement(1071..>1291)
                     /locus_tag="PLV77_09910"
     CDS             complement(1071..>1291)
                     /locus_tag="PLV77_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16206.1"
                     /translation="SSSHIIVRIAAAIPSSLSASTKMPPPARTFGSGEWIVAMTGAPA
                     AAATVVASLTGTRANDLYKALTGREPRR"
BASE COUNT          207 a          445 c          424 g          215 t
ORIGIN      
        1 aacacctctt caccccgctg gcgcatgtgc cgggcgagga tgtcggcggc catggtcgag
       61 taggcgtggc cgagatgggg ctcgccgttg ccgtagaaga tcggtgtggt gacgtagaaa
      121 ctcacgggag atccagcata ggacttgttt cgtccgcgtg gctcagccga ggccggcttc
      181 gacggccgcc gcgaacagtt ccggtccggg cgtctcgagt ccggtggtca gtcccaggta
      241 gaagctcggc tcggtcactt cgacctccat caggacgggc tccccctgcg gcgagctgac
      301 catgtcgatg cgggcgtaga gcggtaccga gccgaatctc cgggccagcc aggcgacgac
      361 cttcgcgccc agttcgacct cggcttcgct cgcgtcggcg aggctcatca gatcctcggc
      421 gaacatccct tccgccacgg tcgtccccgg ccggaccggt gccaccccgg attccggcag
      481 gaaggcgcgc ttcttcaggg cgtaggcgaa gcggccgccg aagaacagca ccgccgtctc
      541 tccccgttcg tcgatccagg gaatgtaggg ctggatcatc gcgatctcgt cctgtcgggc
      601 gagcttctcg aggagggcga ggccgccttc ctcgttcgct tcgtcaaaga ctcccgtgtc
      661 ccgggcgccg gctccggaaa ccggtttgat caccaccttg ccgccgaagg gcggcgggtt
      721 cgagccggga gcgaccagcg cggtcggcgg gaccggcagc ccggccccgt ccagttcggc
      781 caggtagcgc ttgtcggtgt tccagaggat catcgccggc acgttgcgga gcttccccgg
      841 ccccaccgac tcgcaccagg caaggaattc ctccagccgc tccgtgtaat cccaggtcga
      901 gcggattacc gtcaggtcga aggatcccca atcaaccgcc gggtcattcc agaccgcgaa
      961 ttcgacctcc accccccgtt cggccagcag atcggccgag agcaggtcgt cctcccaatc
     1021 cgaaatcctg tcctcgcagg tggcgtaggc aaccctggac ccgacggcgc tcaacgccgc
     1081 ggttcccggc cggtcagggc cttgtagagg tcgttggccc gggtcccggt cagcgaggcg
     1141 accacggtcg cggccgccgc aggcgcaccc gtcatcgcca cgatccattc gccgctgccg
     1201 aaggtccttg ccggcggagg catcttcgtc gatgccgaca gcgacgaagg gattgctgcg
     1261 gcgatgcgaa cgatgatgtg cgatgacgaa a
//
LOCUS       DAOQMF010000653         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_90846_length_1290_cov_2.657490, whole genome shotgun sequence.
ACCESSION   DAOQMF010000653 DAOQMF010000000
VERSION     DAOQMF010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90846_length_1290_cov_2.657490"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLV77_09915"
     CDS             <1..554
                     /locus_tag="PLV77_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16207.1"
                     /translation="GKDLFVVRLKTIKNVDIRVPVREEPGSLNGLVKSPSPKVLPDGY
                     ELATGFSSIGSISMADYIANRDAKPGKLKVTAATVKGRQLTAKVSCPAAAGSCAKAKL
                     SFKGAAGRGKGVVLATRAGVTARPGKTATVKLNLTARARKLFKDSKKGKRRIKGLKSL
                     KATVLVNGKSAGKITVKRTGKVN"
     gene            complement(686..>1290)
                     /locus_tag="PLV77_09920"
     CDS             complement(686..>1290)
                     /locus_tag="PLV77_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC16208.1"
                     /translation="SGAIAGMGESEEGAVVGILVPTNEEDRLVKVEDALDRELPRSLS
                     GLTVTISRSRLATGPDQIERAAREAQLALNVGEAEGRSPIAFEDTGAYRLLLGTSNEE
                     LMSFYSETVEPLVAYDEQYETDLVATVEAYLDSDANVPATAKQMFTHRHTIRYRLERI
                     RDLSGHDATATEGRERLGLGIKAMRVLGIASPRGRSKERT"
BASE COUNT          222 a          410 c          455 g          203 t
ORIGIN      
        1 cgggcaagga tctgttcgtg gtgcgcctca agacgatcaa gaacgtcgac atcagggttc
       61 cggtgcggga agagcccggc tcgctgaacg gcctggtcaa gagtccgtcc ccgaaggtcc
      121 tgcccgacgg ctacgagctg gcgaccgggt tcagctcgat cggatcgatc agcatggctg
      181 actacatcgc caaccgcgac gccaagccgg gcaagctgaa ggtgaccgct gccacggtca
      241 agggcaggca gctcaccgcc aaagtcagtt gcccggccgc ggccggctcc tgcgcgaagg
      301 caaagctttc cttcaagggc gccgccggca ggggcaaggg cgtagtgctc gccaccaggg
      361 cgggggtgac cgccaggccc ggcaagaccg caacggtcaa gctcaacctg accgccaggg
      421 cgcgcaagct gttcaaggac agcaagaagg gcaagcgcag gatcaagggc ctcaagtcac
      481 tcaaggccac cgtcctggtc aacggcaagt ccgccggcaa gatcaccgtc aagaggaccg
      541 gcaaagtcaa ctagccgcaa ccggcccgga ggagtctccc tgggtcggtg ccgcaggttt
      601 gcgacctctc cggttgcaaa cctgcggcac ctgcccgaat cagggccgga accggtcgag
      661 accggatctg ggtgcgttgc ccgggctagg ttcgttcttt ggagcggccc cggggtgagg
      721 cgatgccgag gacgcgcatc gccttgattc cgaggccgag gcgttcccgg ccttcggtgg
      781 cggtggcgtc gtggccggag aggtcgcgga tacgttcgag gcggtagcgg atcgtgtgcc
      841 ggtgggtgaa catctgcttg gcggtggcgg gaacgttcgc atcgctgtcc agataggcct
      901 cgaccgtggc gaccaggtcg gtttcatact gctcgtcgta ggcgaccagt ggctcgaccg
      961 tctccgaata gaagctcatc agttcctcgt tgctggtgcc gagcagcagg cggtaggcgc
     1021 ccgtgtcctc gaaggcgatc ggggagcggc cctcggcctc gcccacgttg agcgcgagct
     1081 gggcttcccg ggcggcccgc tcgatctgat ccggcccggt ggccaggcgc gagcgggaaa
     1141 tggttacggt caggcccgag agcgagcgcg gaagttcccg gtcgagcgcg tcctcgacct
     1201 tgaccaggcg gtcttcctcg ttggtcggga ccaggatgcc gaccacggcc ccttcctcgg
     1261 attcacccat gccggcgatc gcgccggagg
//
LOCUS       DAOQMF010000654         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_91122_length_1288_cov_2.410381, whole genome shotgun sequence.
ACCESSION   DAOQMF010000654 DAOQMF010000000
VERSION     DAOQMF010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91122_length_1288_cov_2.410381"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLV77_09925"
     CDS             complement(<1..225)
                     /locus_tag="PLV77_09925"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNC16209.1"
                     /translation="MVILAFVAQRVISPDSGDKPPTYNEFLTMVDKGEIDSVTVNTKN
                     NSLDVTEKAADGSDSEGEKFETGYPPNTEQS"
     gene            complement(398..961)
                     /gene="hpt"
                     /locus_tag="PLV77_09930"
     CDS             complement(398..961)
                     /gene="hpt"
                     /locus_tag="PLV77_09930"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNC16210.1"
                     /translation="MAATPPNELVSVGEVLITAEDLQRKVADLGAEVSRDYAGKDLVM
                     VGVLRGAVVFIADLLRHLTVPCEIDFMAVSSYGSSRDSSGVVRILKDLDDSITGRDVL
                     IVEDIVDSGLTLNYLMRNLQARDPASLEVCTLLTKPERRRVDVPIRYTGFEIPNQFVV
                     GYGLDFAQRYRNLDHVALLPEDAVPDR"
     gene            complement(980..>1288)
                     /gene="tilS"
                     /locus_tag="PLV77_09935"
     CDS             complement(980..>1288)
                     /gene="tilS"
                     /locus_tag="PLV77_09935"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNC16211.1"
                     /translation="LDLDDLLIWLIEKSPGPDDLLLQVRPWRFGDRIEPLGMSGSKSL
                     QDVFTDALVPASRRQSWPVLVVGEKVIWVPGLVRSRHLLIAGPDREVISLHARPPFSI
                     "
BASE COUNT          240 a          397 c          420 g          231 t
ORIGIN      
        1 gctctgttcg gtgttcggcg ggtagccggt ttcgaacttc tcaccctcgg agtcggagcc
       61 gtcggcggcc ttttcggtca cgtcgagcga attgttcttg gtgttgaccg tgaccgagtc
      121 gatctcgccc ttgtcgacca tggtcaggaa ctcgttgtag gtcggcggct tgtcgccgct
      181 gtccgggctg atcacgcgct gcgcgacgaa ggccagaatg accaccagca ggatggggaa
      241 tgccgcgctc ttgaaaaacc gtttcaaatc ttgggtctct ttccacgacc tcgcgacagg
      301 ctctacctgc cttcaggccg aattgtcaga ctagcaggga gaacccggca accggcatgg
      361 gagagcggtc ccggatgaag atttcttgcc gccgccgcta acggtcgggg accgcgtctt
      421 ccggcagcaa agccacatga tcgagattcc ggtagcgctg ggcgaaatcg agcccgtaac
      481 cgaccacgaa ctgattgggg atctcgaaac cggtgtagcg gatcggaacg tcgacccggc
      541 gccgttccgg cttggtcagg agggtgcaca cctccagtga cgccgggtcg cgggcctgga
      601 ggttccgcat caggtagttg agcgtgaggc ccgaatcgac gatgtcctcg acgatcagca
      661 cgtcacggcc ggtgatcgag tcatccaggt ccttgaggat ccgcaccacg cctgaagaat
      721 cacgcgacga gccgtagctg gagaccgcca tgaagtcgat ctcgcagggc acggtgaggt
      781 gccggagcag gtcggcgatg aagaccaccg cgccgcgcag caccccgacc atgaccaggt
      841 ccttgccggc gtaatcgcgg ctgacttcgg ccccgaggtc ggcgactttc cgctggaggt
      901 cttcagcggt gatcagcacc tcgccgaccg agacgagttc attcggaggc gtcgcagcca
      961 tcgccgccag tctagaggtc tagatggaga acggcggccg ggcgtggagg ctaatgactt
     1021 cccggtccgg ccctgcgatc aggaggtggc gtgaccggac cagccccggc acccagatga
     1081 ccttctcgcc gaccaccagg accggccagc tctgccttct ggaagcgggc acgagtgcgt
     1141 cggtgaagac gtcctggagg ctcttgctgc cgctcatgcc aagcggctcg atgcggtcgc
     1201 cgaaacgcca cggtcgcacc tgaagcagaa ggtcgtcagg acccggtgac ttctcgatca
     1261 accagatgag caggtcatcg agatcgag
//
LOCUS       DAOQMF010000655         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_91184_length_1287_cov_3.899351, whole genome shotgun sequence.
ACCESSION   DAOQMF010000655 DAOQMF010000000
VERSION     DAOQMF010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91184_length_1287_cov_3.899351"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLV77_09940"
     CDS             <1..291
                     /locus_tag="PLV77_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC16212.1"
                     /translation="QTFWRITLPLTMPGIIAGILLTFIPAAGDYVNATFLGGTNQAMI
                     GNVIQSKYLVVNDYPDAAALSFTLMAIILVALLFYIKFAGSEALMGEEEETR"
     gene            288..1136
                     /locus_tag="PLV77_09945"
     CDS             288..1136
                     /locus_tag="PLV77_09945"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC16213.1"
                     /translation="MKAWRWTKDHMVLFVGLLALAYMLIPILVIAVFSFNNPEGRYNF
                     EWVGFTTEYWGKPFAITELTDALLVSIRLAFFTAIGATILGTLIAIALVRYQFFGRRA
                     ANLLIVIPMATPEVVIGASLLSMFLIYGAKLGFGTLLIAHIMFSISFVVIVVRSRLIG
                     FDRRLEEAAADLGANAFTTFRKVTLPLIAPGVISAALLSFALSIDDFVISNFNSGSTV
                     TFPLYIFGANQRGIPVQVNVIATMLFAVTVIAMIAVILQQRRAEKAAGLRPDEIADKK
                     AISLPV"
BASE COUNT          204 a          427 c          390 g          266 t
ORIGIN      
        1 cagacgttct ggcggatcac ccttccactg accatgcccg gcatcatcgc cggcatcctc
       61 ttgacgttca tcccggcggc cggtgactac gtcaacgcga cttttctcgg cggcaccaac
      121 caggcgatga tcggcaacgt gatccagtcg aagtacctgg tggtcaacga ttatccggat
      181 gcggcggcgc tctccttcac gctgatggcg atcatcctcg tcgcactcct gttctacata
      241 aagttcgccg gttcggaagc gttgatgggc gaagaggagg agacacgatg aaagcctggc
      301 gctggaccaa ggatcacatg gtgctcttcg tcgggttgct tgccctcgcg tacatgctga
      361 tcccgatcct cgtcatcgcg gtcttctcgt tcaacaaccc ggagggccgt tacaacttcg
      421 agtgggtcgg cttcaccacc gaatactggg gcaagccctt cgcgatcacc gaactgaccg
      481 acgcactgct ggtcagtatc cggcttgcct tcttcaccgc aatcggtgcc acgatcctcg
      541 gcactctgat cgcaatcgcc ctggtccggt accagttctt cgggcgtcgg gcggcgaacc
      601 tcctgatcgt gatcccgatg gcgacccccg aggtcgtgat cggtgcgtcg ctgctctcga
      661 tgttcctgat ctatggcgcc aagctcgggt tcggcacgct gctgatcgcc cacatcatgt
      721 tctcgatcag cttcgtggtg atcgtggtga ggtcgcgtct gatcggattc gaccgacggc
      781 tcgaggaagc ggcggcagac ctcggggcga atgccttcac gacttttcgc aaggtgaccc
      841 tgccgctgat cgcgccaggg gtcatctcgg ccgccctgct ttccttcgcc ctctcgatcg
      901 acgacttcgt gatctcgaac ttcaactcgg gctccaccgt gaccttcccg ctctacatct
      961 tcggcgccaa ccagcgcggc atcccggtcc aggtcaacgt gatcgcgacc atgctcttcg
     1021 ccgtcaccgt gatcgcgatg atcgccgtca tcctgcagca gcgccgggcc gagaaggcag
     1081 cgggcctgcg gccggacgag atcgccgaca agaaagccat ctccctcccc gtctgaccct
     1141 gttggtgacc acgccgcggg cccgagtggg ccccggtggt tacgccgtgg gatcgggtgg
     1201 ttacgccgcg ggggccggtg gttacgccgc ggggtcgggt gggggtagcc gggacggacg
     1261 cacgcgcggc attcagccgc gctggtc
//
LOCUS       DAOQMF010000656         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_91206_length_1287_cov_3.191558, whole genome shotgun sequence.
ACCESSION   DAOQMF010000656 DAOQMF010000000
VERSION     DAOQMF010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91206_length_1287_cov_3.191558"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1287)
                     /locus_tag="PLV77_09950"
     CDS             complement(<1..>1287)
                     /locus_tag="PLV77_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16214.1"
                     /translation="LHAYDDPAQRVMLIDAATGERQPIWTELDANPTSTDPGEDDPGG
                     INTNPSNTTPVNLIVRPARNLEWGHRYIVAFRDLRDASDNPIQSPLGFRVYRDELPTR
                     QAIVEARRDHMNSVIGDLVDKAGVERSSLYMAWDFTVASQGSVTSRALQVRDDAFARL
                     GDNNLGNRVIEGSSPDVQIEYVCDRYENPDSENPVSDGCNGGPNPPSGTDRRPDVPGS
                     DIIRVIDGEILDVPCYLNQNGCPSGATFEFKPDGSIDFNENFTTDVPFRCFIPSSVQP
                     AGQNTAVTPGQAGIYGHGLLGDRYQVTSAGPVNVATQGGSVWCGADWQGFSTGDLLSV
                     FQALGDMSNFPKMADRMLQGFLNFMMIQRAMIHPDGFADLPAFQLDPDGPGGEDPVSV
                     IDVSAGANTRGVYYGISQGGIMGGALMALTPDSDYG"
BASE COUNT          202 a          392 c          461 g          232 t
ORIGIN      
        1 ccccgtagtc ggaatccggg gtaagggcca tcagggcgcc gcccatgatg ccgccctggc
       61 tgatcccgta atagacgccg cgggtgttcg ctccggcgga cacgtcgatc accgaaaccg
      121 gatcctctcc tccgggtccg tccgggtcga gctggaaggc gggcaggtcc gcgaagccgt
      181 ccgggtggat catcgcgcgc tggatcatca tgaaattgag gaatccctgg agcatccggt
      241 cggccatctt cgggaagttg gacatgtcgc cgagggcctg aaacacggaa aggagatccc
      301 cggtcgagaa gccctgccag tcggcgccac accagacgct tccgccctgg gtggcgacgt
      361 tgaccgggcc ggccgaggtc acctggtagc ggtcaccgag cagcccgtgg ccgtagattc
      421 cggcctggcc cggggtcacg gccgtgttct gtccggccgg ctgcaccgaa ctgggtatga
      481 agcagcggaa cggcacgtcg gtcgtgaagt tctcgttgaa gtcgatcgac ccgtccggct
      541 tgaactcgaa ggtggccccg gaagggcagc cgttctggtt caggtaacag ggaacgtcga
      601 ggatctctcc gtcaatcacg cggatgatgt cggagccggg aacgtcgggg cgccggtcgg
      661 ttcccgatgg cgggttcggg ccaccgttgc agccgtccga aaccgggttc tccgaatcgg
      721 ggttctcgta gcggtcacag acgtactcga tctggacgtc ggggcttgag ccctcgatca
      781 cccggttgcc gaggttgttg tcacccagcc gggcgaaggc gtcgtcgcgg acctgcaggg
      841 ccctcgaggt gacgctcccc tgagaggcga cggtgaagtc ccacgccatg taaaggctcg
      901 agcgctcgac cccggccttg tcgaccagat cgccgatcac cgagttcatg tggtcgcgcc
      961 gcgcttcgac gatcgcctgc cgggtgggca gctcgtcacg gtagacgcgg aatccgagcg
     1021 gcgactggat cgggttgtcg ctggcgtccc tgaggtcgcg gaaggcgacg atgtaacggt
     1081 ggccccactc gaggttccgg gccggtcgga cgatcaggtt gaccggtgtg gtgttggaag
     1141 gattcgtgtt gattccgccc gggtcgtcct cgcccggatc ggtcgaggtc gggtttgcgt
     1201 cgagttcggt ccagatcggc tggcgctcgc cggtggcggc atcgatcagc atgacccgct
     1261 gggcggggtc gtcgtaggca tgaaggt
//
LOCUS       DAOQMF010000657         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_91317_length_1286_cov_4.432169, whole genome shotgun sequence.
ACCESSION   DAOQMF010000657 DAOQMF010000000
VERSION     DAOQMF010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91317_length_1286_cov_4.432169"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..632
                     /locus_tag="PLV77_09955"
     CDS             111..632
                     /locus_tag="PLV77_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16215.1"
                     /translation="MNYDRISPWLISGVLAVLMAGLAGWAIGAGAANGESDASAARKD
                     GFREGFALVFPDARSATAGRGFKEGVHRGRTAGAKTGSREGATIGAGNVEIEQAVDSE
                     KAAEAAASAAQSEIAARQPNCGIVPDAPSWCPTSDELASYKAAVKAAEEAAKEAEKQK
                     QEDQKPGKANGFP"
     gene            619..1044
                     /locus_tag="PLV77_09960"
     CDS             619..1044
                     /locus_tag="PLV77_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16216.1"
                     /translation="MDSPEPEESEPGGRPWMIPALAGVALLLAAAGIGFGIGKTTVSR
                     DPGPDLTRAEAFVQARKKAEKEVSRQMARRGFDAGRRSGHSHGIIAGGMAAESAVTIQ
                     FRQQRASVAQSDAAEVQSELAGMTAAPAPPVPTPEPDDG"
     gene            complement(1052..>1286)
                     /locus_tag="PLV77_09965"
     CDS             complement(1052..>1286)
                     /locus_tag="PLV77_09965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNC16217.1"
                     /translation="VLFLLLGIAQLLTRLLDRKSEFEADLYSAGIGYRQEMIGALTEL
                     REIYGDPEPNKPWSPARLGSTHPPFTDRIAALG"
BASE COUNT          233 a          422 c          458 g          173 t
ORIGIN      
        1 ctcgccttcc gcaagtcata ttcggcctcc tacaagcgca actacatccg ggcctaccag
       61 gacgccggga tggacccgcc gaagccggaa gacgtcgaag tccccgaacc atgaactacg
      121 accggatctc cccctggctg atctcaggcg tgctggccgt cctgatggcc gggctggccg
      181 gctgggctat cggggcaggg gcggcaaacg gcgagagtga cgccagcgcc gcccgcaagg
      241 acggttttcg ggaaggattc gcgctggtct tccccgacgc acggtcggcc accgccggtc
      301 gggggttcaa ggagggcgtc caccggggaa ggacggccgg tgcgaagacc ggctcccggg
      361 aaggagccac gatcggcgcc ggcaacgtcg agatcgagca ggcggtcgat tcggagaagg
      421 ccgccgaggc ggccgcgtcg gcggcccagt cggagattgc ggcccgccag ccgaactgcg
      481 ggatagtccc ggacgcgcct tcctggtgcc cgaccagtga cgaactcgct tcctacaagg
      541 ccgcggtcaa ggcagccgag gaagcagcca aggaagcaga gaagcaaaag caggaagatc
      601 agaagccggg caaggccaat ggattcccct gaaccggaag aaagcgaacc ggggggaagg
      661 ccctggatga ttcccgccct ggccggtgtt gccctgttgc tcgccgcggc ggggatcggt
      721 ttcgggatcg gcaagaccac cgtttcccgt gatcccgggc ctgacctgac ccgggccgag
      781 gccttcgttc aggcccggaa gaaagcggaa aaggaggtga gccgccagat ggcccggcgc
      841 ggcttcgacg caggccgccg ttccggccac agccacggga tcatcgccgg cgggatggcc
      901 gccgaatccg ccgtgactat ccagtttcgc cagcaacgcg cgtcggtcgc ccagtccgac
      961 gcagccgagg tccagtcaga gctcgccggg atgaccgccg cccccgcgcc gccggtcccg
     1021 actcccgaac ccgacgacgg ctgaccccgg ctcagcccag ggcggcgatt cgatcggtga
     1081 agggcgggtg ggtggagccc aggcgggccg gtgaccacgg tttgttgggt tccgggtcac
     1141 cgtagatttc acgcagttcg gtcagggctc cgatcatctc ctggcgatag ccgatcccgg
     1201 ccgagtagag gtcggcttcg aactcggact tgcggtccag cagccgggtc aggagctggg
     1261 cgatgccgag cagcaggaac aggacg
//
LOCUS       DAOQMF010000658         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_91480_length_1285_cov_3.360976, whole genome shotgun sequence.
ACCESSION   DAOQMF010000658 DAOQMF010000000
VERSION     DAOQMF010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91480_length_1285_cov_3.360976"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLV77_09970"
     CDS             <1..355
                     /locus_tag="PLV77_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16218.1"
                     /translation="MARRAAWAKDLFGGLDGFDLVHDAPVVREFAVKSTAKPLKELNY
                     GPTPGIDPMLPFPVYPLGRDYPEYEDCFLIAITERTSADEIGQMADDMEQCAKGAFST
                     SAIYVGDPGGEHSHA"
     gene            348..>1285
                     /gene="gcvPB"
                     /locus_tag="PLV77_09975"
     CDS             348..>1285
                     /gene="gcvPB"
                     /locus_tag="PLV77_09975"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNC16219.1"
                     /translation="MPELTETPQQADRAKTIYEKSVPGRRAFQLPPLDDLIEEKPLED
                     LIPKHLLREKPAELPEVTEPEIIRHYNRLSRRNFDLDTGFYPLGSCTMKYNPRLNERV
                     AALPGHAQLHPATDPALAQGALQLMYELQEALAEITGLPTVSLQPSAGSQGELAGLLL
                     TRAYHADQGRNPTKVLTPDTAHGTNPASVTMAGYEVVKVATNEQGGVDVEDLKSKIDD
                     DVACLMLTNPNTLGVFDENISEIASLVHDAGGLLYYDGANLNAIMGQARPGDMGFDIV
                     HVNLHKSFSQPHGGGGPGAGPIAVSERVEPFIPRPQV"
BASE COUNT          247 a          439 c          395 g          204 t
ORIGIN      
        1 gatggcgagg cgggccgcct gggccaagga cctatttggc ggcctcgacg gcttcgatct
       61 ggtccacgac gcaccggtcg ttcgcgaatt cgcggtcaag tcgaccgcca agccgctgaa
      121 ggaactgaac tatgggccga cgccgggaat cgacccgatg ctgcctttcc cggtttatcc
      181 gctcggccgc gactaccccg aatacgagga ctgcttcctg atcgcgatca ccgaacgcac
      241 ctccgccgac gagatcggcc agatggccga tgacatggag caatgcgcga agggcgcgtt
      301 ctcgacatcg gccatctatg tgggagatcc cggaggggag cactcgcatg cctgagctga
      361 ccgagacccc gcagcaggcc gatcgcgcga agacgatcta cgagaagtcc gttcccggcc
      421 gccgcgcctt ccagttgccg ccgctcgacg atctgatcga agagaagccg ctcgaggatc
      481 tgatcccgaa acacctgctg cgagagaagc cggccgagct gccggaggtg accgagccgg
      541 agatcatccg gcactacaac cggctgtcgc gtcgaaactt cgatctggac acaggctttt
      601 acccgctcgg ttcctgcacc atgaagtaca acccgcgcct caacgaacgg gtcgcggcgc
      661 tgcccggcca cgcccagctt cacccggcga cggatccggc tcttgcgcag ggcgcgcttc
      721 aactgatgta cgaacttcaa gaagcgctgg ccgagatcac cgggctcccg accgtcagtc
      781 tccagccttc ggccggctcc cagggtgaac tggccgggct gcttctgacc cgcgcctatc
      841 acgctgatca gggtcggaac ccgaccaagg tcctgacccc cgacaccgcc cacggcacca
      901 accccgcctc ggtcacgatg gccggttacg aggtagtcaa ggtggccacc aacgagcagg
      961 gcggggtgga tgtcgaggac ctgaagtcga agatcgacga tgacgtcgcc tgcctgatgc
     1021 tgaccaaccc gaacaccctc ggggtcttcg acgagaacat cagtgagatc gcctcactgg
     1081 tccacgacgc tggcgggctg ctctattacg acggggccaa cctgaacgcg atcatggggc
     1141 aggcccgtcc gggcgacatg ggcttcgaca tcgtccacgt caacctgcac aagtccttct
     1201 cgcaacccca tggcggtggt gggcccgggg ccgggccgat cgccgtgtcc gaaagggtcg
     1261 agcccttcat tcccaggcca caggt
//
LOCUS       DAOQMF010000659         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_91942_length_1282_cov_2.695192, whole genome shotgun sequence.
ACCESSION   DAOQMF010000659 DAOQMF010000000
VERSION     DAOQMF010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91942_length_1282_cov_2.695192"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLV77_09980"
     CDS             complement(<1..422)
                     /locus_tag="PLV77_09980"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNC16220.1"
                     /translation="MSFRFTTAGESHGPGLVTIVEGLPAGLELDRETLDSDMARRQLG
                     HGRGGRMKIETDQVEIRAGVRHGFTMGGPVAALVANRDYKNWDERMNPWPIEGEIPPV
                     TMPRPGHADLVGMWKYKHDDLRPILERASARETAARVAA"
     gene            521..1000
                     /locus_tag="PLV77_09985"
     CDS             521..1000
                     /locus_tag="PLV77_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16221.1"
                     /translation="MLILLGISSVIAFVVPEPQRNDPATDQAPVTGTTGSTGVPAESG
                     ATGKAGTTGEDGTGKRKGEPKVSEPEAARSGGVPIETVTLGGEKPARIEAEAGTRMII
                     TVRSGEGSDVEIEDLGLAGFADPYAPAVFDVILPPEPGRYPIGAPGEKPSAVIIASS"
     gene            complement(1134..1259)
                     /locus_tag="PLV77_09990"
     CDS             complement(1134..1259)
                     /locus_tag="PLV77_09990"
                     /inference="COORDINATES: protein motif:HMM:NF036850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16222.1"
                     /translation="MQDGAKTVDGLEILVRQGARSFEIWTGKRPDLESMRQAART"
BASE COUNT          204 a          411 c          435 g          232 t
ORIGIN      
        1 gcagcaaccc tggcggccgt ttcgcgggca ctggcccgct cgaggatcgg ccggagatca
       61 tcgtgcttgt acttccacat cccgaccagg tcggcgtgac cgggccgcgg catggtgacc
      121 ggcgggatct ccccttcgat cggccacggg ttcatccgct catcccagtt cttgtaatcg
      181 cggttcgcga cgagggcggc gaccggcccg cccatcgtga acccgtgccg cacgccggcc
      241 cggatttcaa cctggtcggt ttcgatcttc atgcggccgc cgcggccgtg gccgagctgg
      301 cgacgcgcca tgtcgctgtc aagcgtctcg cggtccagtt cgagcccggc gggcaggcct
      361 tcgacgatgg ttacgaggcc gggtccgtgg gactcgcctg cggttgtgaa tcggaaactc
      421 attgaggctt ctaggctatt gacaactgca tgcccgacgc tgtctcaacc aacccggggt
      481 ccgacccctg catatggctg cccgtcgact gctgatcgta atgctgatcc tcctggggat
      541 ctcgtcggtg atcgctttcg tggtcccgga gccgcagcgc aacgatccgg cgaccgatca
      601 ggcgccagtt accggaacca caggctcaac gggcgtcccg gccgaaagcg gggcgacggg
      661 caaagccggc acgaccggtg aagacggcac gggcaagcgc aaaggggagc cgaaagtatc
      721 cgagccggag gccgccaggt ccggcggagt cccaatcgaa acggtgactc tcggtggcga
      781 gaaaccggcc agaattgagg cggaagccgg gacgcggatg atcatcacgg tcaggtccgg
      841 cgagggttcg gacgtcgaga tcgaggatct tggcctggcc gggttcgccg acccctacgc
      901 gccggcggtc tttgacgtga tcctcccgcc cgaacccggc aggtacccga tcggcgcccc
      961 gggcgagaag cccagcgcgg taatcatcgc cagctcctga gccaaatctc cggtgggtcg
     1021 gatttccgtc atgtgtgctg gtaatccgac ccacctggtg ctggcgggct ggtcggttgc
     1081 ccggggtggg tcggatttac gtcgtgtggg cgggaaatcc gacccactgg aagtcaggtt
     1141 cgggctgcct ggcgcattga ttcgaggtcc ggtctttttc cggtccagat ttcgaaggac
     1201 cgggccccct ggcggaccag gatttcgagc ccgtcgacgg tcttagctcc gtcctgcatg
     1261 gcggtggcga tcaatgttcc cg
//
LOCUS       DAOQMF010000660         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_92081_length_1281_cov_2.840946, whole genome shotgun sequence.
ACCESSION   DAOQMF010000660 DAOQMF010000000
VERSION     DAOQMF010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92081_length_1281_cov_2.840946"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1281
                     /locus_tag="PLV77_09995"
     CDS             <1..>1281
                     /locus_tag="PLV77_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16223.1"
                     /translation="KAGTEAAGAIQDAFFWMLNQIFGGIQATLSVKLLTWLTTLPDFS
                     GGQVAKIAKSMQIAAGAFLGCILTISIIRFWLGSYTSSGNASSGFEGLIRTGFAALLI
                     GLWPRLFTMGVELANAFSAALLNDAAKQRLENLFQGLDMASLGLPIAGGATAGAVGLF
                     VGAGISILVWIVIATASAVLFLGLIIMKIIVTAGTVIAFVAMPLALVLWPIPETSWIA
                     NMLAKAIAVLLAIPVLWILVFSAASAIGSDVFFLSNNGKNPDFLQTGLNILLIKPLVA
                     CALLYLAVMLPSRLVRMVPLAGGAKVPGSGTARAVTSYATYRSLDAVAGNSALRAPAN
                     AARQGVMDGFGSTGQGASGEPELTGVSAKAFGAGQKMGGQARQVFDRGKQMVGQQGGQ
                     AAGAAAGGSVAGPTGAAVGGAAGGAVGEKAASAV"
BASE COUNT          219 a          406 c          396 g          260 t
ORIGIN      
        1 agaaagcagg taccgaagcc gccggtgcga ttcaggacgc gttcttctgg atgctcaacc
       61 agatcttcgg tggaatccag gccacccttt cggtgaagct cctcacctgg cttacaaccc
      121 ttcccgattt ctccggtggc caggtcgcga agatcgccaa atcaatgcag atcgccgccg
      181 gcgcgtttct cggctgcatc ctcaccatct cgatcatccg gttctggctt ggttcataca
      241 cctcaagcgg caatgcctca tccggctttg aaggactgat ccgaaccggc tttgccgccc
      301 tcttgatcgg tctttggccg aggctcttca cgatgggggt tgagctcgcc aacgctttct
      361 ctgcggcact cctaaacgac gccgccaaac aacgcctcga gaaccttttt caaggcctcg
      421 acatggcctc gctcggcctt ccgattgcag gcggcgcgac cgccggggct gtcggcctgt
      481 tcgtcggggc cggtatctcg atcctggtgt ggatagtcat agccaccgct tccgcggttc
      541 tctttctcgg cttgatcatc atgaagatca tcgtcactgc cggaacggtg atcgcgttcg
      601 ttgcgatgcc tctggccctg gtgctctggc cgattccgga gaccagctgg atcgccaaca
      661 tgctcgcgaa agcgatcgct gttctgctcg ccatcccggt gctttggatt ctcgtcttct
      721 cggctgcctc ggcgatcggc tcggatgtgt tcttcctgtc aaacaacggc aagaaccctg
      781 acttcctcca aacgggcctg aacatcctgc tgatcaaacc cctggtggcc tgtgcgctgc
      841 tctatctagc ggtgatgctg ccgagccggc tggtgagaat ggttccgcta gcgggcgggg
      901 cgaaagtacc cggatcagga acagccaggg ccgtcaccag ctacgccacc tatcgcagcc
      961 tcgacgcggt cgctggcaac tcggcattgc gtgcccctgc caacgcggca aggcagggcg
     1021 tgatggacgg attcggttca accgggcagg gagcctccgg tgagccagaa ctgaccggtg
     1081 tcagcgcgaa agcattcggg gcgggacaga agatgggtgg ccaggcccgc caggtattcg
     1141 accggggcaa gcaaatggtc ggccagcaag gaggtcaggc agccggggct gcggccggcg
     1201 gatccgttgc cggacctacg ggggcggcag tcggcggcgc ggccggaggc gccgtcggcg
     1261 agaaggctgc atcagctgtc c
//
LOCUS       DAOQMF010000661         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_92280_length_1280_cov_2.078367, whole genome shotgun sequence.
ACCESSION   DAOQMF010000661 DAOQMF010000000
VERSION     DAOQMF010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92280_length_1280_cov_2.078367"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLV77_10000"
     CDS             <1..1045
                     /locus_tag="PLV77_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC16224.1"
                     /translation="LIGGFLVEHFWWGAVFLINVPIVLVIIPVAIRLLPESRAAEPPP
                     WDGFAVIMTVIGILGIAFGLKEGARYGFLDPKVLLSLLVGSGSLFWFTLRQLRAPEPL
                     LDVRLFKRREFTAAVGCVFLSMFGLVGIEFFGAQYLQLVLGLEPLEAALRLLPLMLST
                     LAGGLLAARLLGRLGTRWTISLGLAGTALGLVPMLALGLTDQYYLLWPSFLVIGFTLE
                     VALVAANDVIISSVPADEAGQAAAIEETAYELGGGFGVAVLGSILAIVYTAKIGPIEG
                     VTGAGIANAEESLARALEIAGKMASRTADPLIEAAKLAFTSGYHTAIAVSIILVGASA
                     GLAAVLLRPRTPQ"
     gene            complement(1079..>1280)
                     /locus_tag="PLV77_10005"
     CDS             complement(1079..>1280)
                     /locus_tag="PLV77_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16225.1"
                     /translation="SYDRKLEKPSTLEFSLSKAKPKATIAPVRSRTRKWAPKLTIKST
                     PKPAWVRVLDPVGNSSPWVSVR"
BASE COUNT          163 a          417 c          434 g          266 t
ORIGIN      
        1 gctgatcggc ggtttcctgg tcgagcattt ctggtggggt gccgtgttcc tgatcaacgt
       61 gccgatcgtg cttgtgatca tccccgtcgc aatcaggctg ctgcccgaaa gccgggcggc
      121 cgaaccgccg ccctgggacg gcttcgcggt gatcatgacc gtgatcggaa tcctcggaat
      181 cgccttcggc ctcaaggaag gtgcccgcta cggcttcctc gatccgaagg tgctcctctc
      241 gcttctggtc ggttccggat cccttttctg gttcaccctt cgccagctgc gggcgcctga
      301 gcccctgctt gacgtgcggc tcttcaaacg gcgggagttc acggcggcgg tcggttgcgt
      361 cttcctgtcc atgttcggac tggtcgggat cgagttcttc ggggcccagt acctgcagct
      421 ggtgctgggc ctcgaaccct tggaggcggc gctccggctc ttgccgctga tgctttccac
      481 cctggctgga ggcttgcttg ctgcgcgcct gctgggcagg ctcggcaccc gctggacgat
      541 ttcactcggg ctggccggca ccgcactcgg tcttgttccg atgctggcgc tcggcctgac
      601 cgaccagtac tacctgctct ggccttcctt cctggtgatc ggcttcaccc tggaggtggc
      661 cctggtcgcc gccaatgacg tgatcatttc ctcggtcccg gccgacgagg ccggccaggc
      721 ggcggcgatc gaggagacgg cctacgaact cggaggcggt ttcggcgtgg ctgtgctcgg
      781 ttcgatcctg gcgatcgtct acacggcgaa gatcggtccg atcgagggcg tcaccggggc
      841 cgggatcgcc aacgccgagg agtcgctggc acgggcgctc gaaatcgccg gcaagatggc
      901 gagccggacg gctgatccct tgatcgaggc ggccaagctt gccttcacgt ccggctacca
      961 caccgcgatc gcggtctcga tcattctggt cggcgccagc gccggtctgg ccgcggtcct
     1021 gctgcgcccc cggacgccgc agtagccccg caatccggca ggtggctatc gggaggggct
     1081 acctgacgct gacccaggga gagctgttgc cgaccgggtc gagcacccgc acccaggcgg
     1141 gtttgggcgt cgacttgatg gtcagcttcg gcgcccactt gcgggtgcgt gacctgaccg
     1201 gcgcaatcgt cgccttgggc ttcgccttgg agagtgagaa ctcgagggtg gacggtttct
     1261 caagcttgcg gtcgtagctg
//
LOCUS       DAOQMF010000662         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_92282_length_1280_cov_2.062857, whole genome shotgun sequence.
ACCESSION   DAOQMF010000662 DAOQMF010000000
VERSION     DAOQMF010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92282_length_1280_cov_2.062857"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLV77_10010"
     CDS             <1..486
                     /locus_tag="PLV77_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16226.1"
                     /translation="VSGPFNRRRRGPENVITAERFQSWLEEIADTERIRLATWLLVAG
                     CPVSKLTSLRFGIEVHGKGETRKITGVSKDSIEVPAPLEEILANLKGRSPKGQPVLRS
                     ATDPEASIEMPTLRQVWNRFRYGTGPTMRDVHVLGRHYYVDLKDGQVQLTSRTLPKPA
                     R"
     gene            complement(497..829)
                     /locus_tag="PLV77_10015"
     CDS             complement(497..829)
                     /locus_tag="PLV77_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16227.1"
                     /translation="MNGLEELITLILGFARWAAIAGAAVAIAMKTEDFLTGLHETKVE
                     EWRKAYDRYLEQEQAFMEDDNKEALALLLEGRPERPQIHELLVGGTSLATAFISVVLI
                     IQGPDLIL"
     gene            1004..>1280
                     /locus_tag="PLV77_10020"
     CDS             1004..>1280
                     /locus_tag="PLV77_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16228.1"
                     /translation="MSTVDRIRVRSAVNKLSGAIDEIREEQKAISETDPESHLGPLLA
                     EKIGELENLRLRLLFAVAFDDFELTVEPSRSGDLANADGNIYRDGELL"
BASE COUNT          281 a          394 c          360 g          245 t
ORIGIN      
        1 gtgtcaggac cgttcaaccg acggcgccgt ggtcccgaga acgtgatcac cgccgaacgc
       61 ttccagagct ggctagagga gatcgcggat accgaacgaa tccggctggc cacctggctg
      121 cttgttgccg gctgcccggt cagcaagctg acctccctgc gattcggcat cgaagttcac
      181 gggaaaggag aaacgagaaa gatcaccggt gtcagcaagg acagcatcga ggtaccagcc
      241 ccactcgagg aaatactcgc gaacctcaaa ggccggtccc cgaaaggaca accagtgctt
      301 cgttcagcaa ccgatccgga agcgtcgatc gagatgccga ccctgcgaca ggtctggaac
      361 cggttccgct atggcaccgg gcccacgatg cgcgacgtcc atgttctcgg ccgccactac
      421 tacgttgatc tcaaagacgg ccaggtccag ctcacctccc gaacgctccc gaagccggcc
      481 cgctaaatcg ttcaactcac aggatcaggt cgggcccttg aataatcagg actactgaaa
      541 tgaacgctgt ggccagggat gtgccgccca ccagtagctc atggatttgc ggtctttcag
      601 ggcgcccctc aagcaggagc gcaagggctt ccttgttgtc atcttccatg aaagcctgtt
      661 cctgctccag atagcggtca taggcttttc gccattcctc aaccttggtt tcgtgaagtc
      721 cggtaaggaa atcctcggtc ttcatcgcga tggccaccgc ggcgccggcg atagccgccc
      781 acctggcgaa gccgagaatc agcgtgatca gttcttcaag cccgttcatg gcggcaaagt
      841 acctcaccgt gcgaacaacg acgtcggtcg gctgcgattc gggcagctcc ggttcaggtc
      901 cgcccgaccc ggcagaatcg tggtggccat gtccgacgag ggcctcagct tgatcgacat
      961 tttcaacaga gaaatggcag cctgccttga cgttctaaac ggcttgtcaa ctgtcgaccg
     1021 gatccgagtg cgatcagcgg tcaacaaact gagcggcgcg atcgatgaga tccgtgaaga
     1081 acagaaagcc atctccgaaa cagatcccga atcccatctt ggccccttgc ttgccgagaa
     1141 gatcggtgaa ctggagaacc ttcgccttcg tttgcttttc gcagtggcgt tcgatgactt
     1201 cgaactgacc gtggaaccct caagatctgg cgatctggcg aacgccgacg gcaacatcta
     1261 ccgggacggg gagcttctcg
//
LOCUS       DAOQMF010000663         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_93043_length_1274_cov_2.966366, whole genome shotgun sequence.
ACCESSION   DAOQMF010000663 DAOQMF010000000
VERSION     DAOQMF010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93043_length_1274_cov_2.966366"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..740
                     /locus_tag="PLV77_10025"
     CDS             51..740
                     /locus_tag="PLV77_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC16229.1"
                     /translation="MQIENFLFSVSDALRWPVLLALLLALAWSIVEIGILLAEIWRRR
                     WRSINALENAVDQAEVDIVQGDQIGAISALSSVSWSRPMRETMTSIVTLRPSPDAENR
                     IAKRLADYDYNSLRRLERTRILVRMGPALGLMGTLIPLAPALAGLADGNVTELTDNLR
                     VAFGVTVAGLLTGALSFGVSLIRDRIYAQDFSDVEFAAANLAPDKHLAGHFVPSPVEH
                     GHSTQVSMGNP"
     gene            740..1141
                     /locus_tag="PLV77_10030"
     CDS             740..1141
                     /locus_tag="PLV77_10030"
                     /inference="COORDINATES: protein motif:HMM:NF021440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HNC16230.1"
                     /translation="MAMKVTAKARSREDRAGDPLDGLVNLFDIGIILSVGFLLAALSS
                     LNLTTEALSQNDTSPANTVPENSVVTQPDETTEQITIEPGQTVVGQGEPLGTVYQLDD
                     GRTILVKPSTGTTGATGATGTTGVTGSTLNP"
BASE COUNT          221 a          452 c          381 g          220 t
ORIGIN      
        1 gctccgctat cttgaccctt cggctgccct aagccccggg cgcctcaagc atgcagatcg
       61 aaaacttcct cttctccgtt tccgacgctc tccgctggcc agtcctgctc gcgctgctgc
      121 tggccctggc ctggtcgatc gtcgaaatcg ggatcctcct ggccgaaatc tggcgccggc
      181 gctggcggtc gatcaacgcg ctcgagaatg cggtcgacca ggccgaagtc gacatcgtgc
      241 agggcgatca gatcggggcg atttcggcac tttcctcggt ttcgtggagc cggccgatgc
      301 gcgagacgat gacctcgatc gtcaccctcc gtccctcccc cgacgcggag aaccggatcg
      361 ccaagcgact cgccgattac gactacaact cactgcgccg gctcgaacgg acgaggatcc
      421 tggtccggat gggccccgca ctcggcctga tgggcaccct gatcccgctg gctccggctc
      481 tggccggact ggcggacggc aacgtgaccg agttgaccga caacctgcgg gtcgcgttcg
      541 gtgtgaccgt ggcgggcctg ctcaccggtg cgctctcctt cggcgtctcg ctgatccgcg
      601 accggatcta cgcccaggac ttttcggatg tcgagttcgc cgccgccaac ctcgcgccgg
      661 acaagcacct ggccgggcat ttcgtgccgt caccggtcga gcacgggcac agcacccagg
      721 tctcgatggg gaatccctga tggccatgaa ggtgacggcc aaggcgagga gccgcgagga
      781 ccgcgccggt gatccgctcg acggcctggt caacctcttc gacatcggga tcatcctctc
      841 ggtcggtttc ctcctggccg cgctttcctc gctcaacctg accaccgagg ccctctcgca
      901 gaacgacacc tcaccggcga acaccgtccc cgagaactcg gtcgttaccc aaccggacga
      961 gaccaccgaa cagatcacga tcgagcccgg ccagaccgtg gtcggccagg gcgagccgct
     1021 cggcaccgtc taccagctcg acgacggcag aacgatcctc gtcaagccct caaccggcac
     1081 gaccggcgcg accggcgcga ccggaaccac cggagtaacc ggctccacgc tcaaccccta
     1141 gccggcaaac cgttttcgag aagtcgagcc tgaacggggt ttcggggata tcgagaaggg
     1201 agttttgcgc atataacgct cccaactccg atttcgattc gacccatcag gtcgagatcg
     1261 gagttgggtg cgct
//
LOCUS       DAOQMF010000664         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_93880_length_1268_cov_4.031327, whole genome shotgun sequence.
ACCESSION   DAOQMF010000664 DAOQMF010000000
VERSION     DAOQMF010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93880_length_1268_cov_4.031327"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLV77_10035"
     CDS             complement(<1..807)
                     /locus_tag="PLV77_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNC16231.1"
                     /translation="MAIGEEIFRPGSSSDGEVRAGILVTGTEVLTATIRDENGPWLSE
                     QLSGLGVELVEILVVADHPGDLARGLENMKSAGIDLIITTGGLGPTADDLTAEVVAAF
                     SGRKMELDEEMEARIAAILARFAANTNLDLSTDALNEANRKQAMIPVGAVALDPVGTA
                     PGLIVPGQAEGDPLVLVLPGPPRELKPMWEAAQKEPMLRQVLDQATRLEKYRLRMFGT
                     PESELAMSLREIEGEGLPFDGLEITTCLRKGEVEVDVRYREAAADAAEQLK"
     gene            complement(865..>1268)
                     /locus_tag="PLV77_10040"
     CDS             complement(865..>1268)
                     /locus_tag="PLV77_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNC16232.1"
                     /translation="GEPEFEQELLSEPETMQEETSDAEFDPDQDDLLNEAIMLVVETE
                     TASVSMIQRRLRVGYTRAGRLIDMLERRGVISGYEGSKPRQVLITHADLPRVLGGGNA
                     GDVEGEPDAEASAGGEIADLPVPSETLDTSD"
BASE COUNT          183 a          466 c          384 g          235 t
ORIGIN      
        1 cttcagctgc tcggcggcgt cggcggccgc ctcccggtag cgcacgtcaa cctcgacctc
       61 gcccttgcgc aggcaggtcg tgatttccag gccgtcaaag ggcagcccct ccccttcgat
      121 ctcgcgcagg ctcatcgcca gctccgactc gggggtgccg aacatgcgca accggtactt
      181 ctccagccgg gtggcctggt ccagcacctg ccgcagcatc ggctccttct gtgccgcttc
      241 ccacattggc ttgagctccc gcggcggccc cggcagcacc aggaccagcg ggtcaccttc
      301 tgcctgaccg ggaacgatca gcccgggtgc ggttccgacc gggtcgaggg ccaccgctcc
      361 gaccggaatc atcgcctgct tgcggttggc ctcgttcagg gcgtcggtgc tcaggtcaag
      421 gttcgtgttg gcggcgaacc tggcgaggat tgccgcgatc cgcgcctcca tttcctcgtc
      481 cagttccatc ttccggccgg agaaggcggc gacgacctcg gcggtgaggt catcggcggt
      541 cgggccgaga ccaccggtgg tgatgatcag gtcgatcccg gccgatttca tgttttcgag
      601 accccgggcc aggtctccgg gatggtcggc gacgaccagg atctcgacca gctcgacccc
      661 cagcccgctc agctgctccg aaagccaggg cccgttctcg tcacggatcg tggcggtcag
      721 cacctcggtc ccggtgacca ggatgccggc cctgacctca ccgtccgagc tgctccccgg
      781 cctgaatatc tcctcgccaa ttgccacggc ctgaccctac agccgcggca ccggttgcgg
      841 cctccgccga agcccggctt ccggctaatc cgaggtgtcg agcgtctcgg acgggaccgg
      901 caggtcggca atttcgccgc ctgcggaagc ttcagcgtca ggttccccct ccacatcgcc
      961 ggcatttccg ccgccaagca cccgcggcag atcagcgtgg gtgatcagca cctggcgggg
     1021 cttcgaacct tcgtaaccgg agatcacgcc ccggcgctcg agcatgtcga tcaggcgccc
     1081 ggcccgggtg taaccgaccc tcagacgcct ttgaatcatc gataccgaag ccgtctcggt
     1141 ctcgaccacc agcatgatcg cctcgttcag caggtcgtcc tggtccggat cgaattccgc
     1201 gtccgaagtc tcttcctgca tcgtctccgg ctcgctgagc agttcctgtt cgaattccgg
     1261 ctcgccct
//
LOCUS       DAOQMF010000665         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_93883_length_1268_cov_3.709810, whole genome shotgun sequence.
ACCESSION   DAOQMF010000665 DAOQMF010000000
VERSION     DAOQMF010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93883_length_1268_cov_3.709810"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PLV77_10045"
     CDS             complement(<1..1046)
                     /locus_tag="PLV77_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNC16233.1"
                     /translation="MERKWLSLVAVITGIFMLLIDITIVNVALPDIGTDLDASFSSLE
                     WVVNAYTLTLASSLLVSGSLADLIGRKKVFAAGLIIFTLASLACALAETSLMLNLLRA
                     VQGIGGGMMFSTSLAIIAQTFQGKDRGIAFGIVGATTGAAVAIGPLLGGLLTDTFGWE
                     AIFFVNVPIGIFALIGTFRNVDESKDPHPSGIDWLGAVLFSAALFLMIYALVHGNDKG
                     WGSLEIIGAMVLSVVLLIIFGGVETHKEHPLFDLSLFRKPAFVGACLVAFCMSSSLFA
                     LFLFLTFYIQGGVLGFSPLEAGLRFLPITGMMLVAGPIAGRLTTKVQVRYLLSGGLLG
                     VALGIFLLHGIDVNS"
     gene            1150..1223
                     /locus_tag="PLV77_10050"
     tRNA            1150..1223
                     /locus_tag="PLV77_10050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1184..1186,aa:Met,seq:cat)
BASE COUNT          263 a          377 c          447 g          181 t
ORIGIN      
        1 gagttcacat cgattccgtg aagcaggaag atgccgagcg cgacgccgag caggccgccg
       61 ctcagcaggt agcgaacctg gaccttggtg gtcaggcggc cggcgatcgg gccggccacc
      121 agcatcatgc cggtgatcgg caggaacctg agaccggcct ccaggggaga gaagccgagc
      181 acgccgccct ggatgtagaa ggtgaggaag aggaagaggg cgaacagcga tgacgacatg
      241 cagaaggcga cgaggcaggc gccgacgaag gccggcttgc gaaagaggct caggtcgaag
      301 agcggatgtt ccttgtgggt ttcgaccccg ccgaagatga tcagcaggac gaccgagagc
      361 accatcgcgc cgatgatttc caggctgccc cagcccttgt cgttgccgtg aacgagcgcg
      421 tagatcatca ggaagagcgc ggccgagaag agcacggcgc cgagccagtc gattcccgac
      481 ggatgcgggt ccttggactc gtcgacgttg cggaaggtgc cgatcagggc gaagatcccg
      541 atcggcacgt tgacgaagaa gatcgcttcc cagccgaagg tgtcggtcag aaggccgccg
      601 agcagcgggc cgatcgcgac cgccgccccg gtggtcgccc cgacgatccc gaaggcgatg
      661 ccccggtcct tgccctggaa agtctgggcg atgatcgcca gggaagtgga gaacatcatc
      721 ccgccgccga tcccctggac cgcacggagc aggttcagca tcagcgaggt ctcggccagg
      781 gcgcaggcca gggaggcgag ggtgaagatg atcagcccgg ccgcgaagac cttcttgcgg
      841 ccgatcaggt cagccagtga tccggagacc agcaggctgg aggcgagagt cagcgtgtac
      901 gcgttgacca cccattcgag gctggagaac gaagcgtcga ggtcggtgcc gatgtccggc
      961 agtgccacgt tgacgatcgt gatgtcgatc agcagcatga agatgccggt gatgaccgcg
     1021 acgagggaga gccacttcct ttccaccttc aggaggctat ccagccggcc tccccggccc
     1081 tgcaggagcg tcaccggcgc gaacggagga aagccccaaa gcccatgagt gctagacttg
     1141 gcggtccgcc gcggggtgga gcagtcaggt agctcgtcgg gctcataacc cgaaggtcgc
     1201 aggttcgaat cctgcccccg ctatggtctt ggaacccgct caccgagcgg gtttcgcggt
     1261 taacgggg
//
LOCUS       DAOQMF010000666         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_93884_length_1268_cov_3.676834, whole genome shotgun sequence.
ACCESSION   DAOQMF010000666 DAOQMF010000000
VERSION     DAOQMF010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93884_length_1268_cov_3.676834"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLV77_10055"
     CDS             complement(<1..223)
                     /locus_tag="PLV77_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNC16234.1"
                     /translation="MLIEKVDLDALGLSYGDATRIQGEVAPAKVSLGGQDYEPVPAEP
                     AFLLEVSRTSAGYALRLRFEVGLSGPCFRC"
     gene            260..799
                     /locus_tag="PLV77_10060"
     CDS             260..799
                     /locus_tag="PLV77_10060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC16235.1"
                     /translation="MSQPDESSYLDSVRGQVPLYDALQDAAVAAVPFEPSRVLELGIG
                     TGETASRLLARFPDANVSGLDSDPDMLFKAREIVDEVSLARMEDPLPDGPWDLILSVL
                     SVHRLTDEQKQTLFRRVREQSKALVIGDFVSLGDDRGETRVEGEAYPDTAEDLAAWCD
                     GKIVWSEDDLVVIVAEYRR"
     gene            complement(888..>1268)
                     /locus_tag="PLV77_10065"
     CDS             complement(888..>1268)
                     /locus_tag="PLV77_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNC16236.1"
                     /translation="EEIYDLLDQMRATIPEEIKQARWIVKERQEMLEEAKRESERVIR
                     EAREQQARLVSDEEVTKQAERAADEIVEDARAREREIRLGAEDYADEILNTLEVNLQK
                     FTAAVQRGRDRLAGRDEPLDEIEE"
BASE COUNT          211 a          403 c          409 g          245 t
ORIGIN      
        1 ggcagcggaa acagggcccg gaaaggccga cctcgaagcg gagcctgagt gcgtaaccgg
       61 cgctggtccg cgagacctcg agcaggaagg caggttcggc cggcaccggt tcgtagtcct
      121 gcccgccgag ggaaaccttg gccggagcca cttcaccctg gattcttgtg gcgtctccat
      181 aggagaggcc gagggcgtcg agatcaacct tttcgatcag catcccttcc cagcatggca
      241 gacgaatacg atgcgctcga tgagccagcc ggacgaaagc agctacctcg actcggtccg
      301 cggccaggtg ccgctttacg acgcgcttca ggatgcggcg gtcgccgcgg tccccttcga
      361 accgtcccgc gttcttgagc tgggaatcgg caccggtgaa acggcttcgc gtctgctggc
      421 ccgttttccc gatgcgaatg tcagcggcct ggattcggat cccgacatgc tcttcaaggc
      481 ccgggaaatc gtcgacgagg tcagtctcgc ccgcatggag gatccgctcc cggacggtcc
      541 gtgggacctg atcctctcgg tcctttcggt gcatcggctg accgacgagc agaagcagac
      601 cctgtttcgc cgggtccgcg agcagtcaaa ggccctggtc atcggcgatt tcgtctcgct
      661 gggcgacgac cggggagaga cccgggtcga aggcgaggcc taccccgaca cagccgaaga
      721 cctggccgcc tggtgcgacg gaaagatcgt ctggtccgaa gacgacctag tagtcatcgt
      781 tgcggaatac cgaagataga gccccaccca accattcctt gattcgaatg ggtggggcgg
      841 gttgctttgg gagggccggc gcagaccggc ccgactgcag ctaagtctta ttcctcgatt
      901 tcgtcgaggg gttcgtcgcg gccggcaagc ctgtcgcgac cgcgctggac ggcggcggtg
      961 aacttctgga ggttcacttc gagggtgttg aggatctcgt cggcgtaatc ctcggcaccg
     1021 agacggatct cgcgctcgcg ggcacgggcg tcttcgacga tttcgtcggc ggcccgctcg
     1081 gcctgcttgg tgacttcctc atcggaaacc agccgcgcct gctgctcgcg tgcctcgcgg
     1141 attacccgct cggattcacg cttggcctct tcgagcatct cctgacgctc cttgacgatc
     1201 cagcgcgcct gtttgatctc ttccgggatc gtggcacgca tctggtcgag cagatcgtag
     1261 atctcctc
//
LOCUS       DAOQMF010000667         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_94311_length_1265_cov_3.050413, whole genome shotgun sequence.
ACCESSION   DAOQMF010000667 DAOQMF010000000
VERSION     DAOQMF010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94311_length_1265_cov_3.050413"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLV77_10070"
     CDS             <1..374
                     /locus_tag="PLV77_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HNC16237.1"
                     /translation="LLRDQRTIAGIGRTWVDEILWSARLSPFKRAADLEPEEAERLLA
                     SIHDRFQAAIDFYESGLTLPLPDKLPKPLEVHGREGEACSRCGELIHAVHYSDYTLCY
                     CPREQTGGEPLKDRRLSRLLK"
     gene            complement(397..657)
                     /locus_tag="PLV77_10075"
     CDS             complement(397..657)
                     /locus_tag="PLV77_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16238.1"
                     /translation="MTGPWRDLEQRLEFENEAERPLRESGEGESEGFEEAEAELIHQS
                     EDPDLGRDPELDAIVEAEDAGSVYSEADEILSSEEPDSDHDG"
     gene            complement(714..1190)
                     /locus_tag="PLV77_10080"
     CDS             complement(714..1190)
                     /locus_tag="PLV77_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16239.1"
                     /translation="MSDRELGIYLNDHLTGATGGVALARRAAANATDQERKAMWKSIA
                     HEVEEDRVTLTHVRDLIAVKPNRAKYALAWTAEKLGRLKLNGRLTGNSELGQMLELEA
                     LVIGVTGKLSLWKALARLEDPRLEGIDFDRLVERAESQRRRLEQFRINRVPAAIGR"
BASE COUNT          201 a          442 c          389 g          233 t
ORIGIN      
        1 ccctgctgcg cgaccagcgg acgatcgccg ggatcggccg cacctgggtt gacgaaatcc
       61 tctggagcgc ccgcctctcc ccgttcaagc gggcggccga ccttgagccg gaggaagcgg
      121 aacggttgct tgcgtcgatc cacgaccgct tccaggcggc gatcgatttc tacgaatccg
      181 ggctcaccct gccgctaccg gacaaactgc cgaagccgct cgaggttcac gggcgtgagg
      241 gtgaagcctg ctcgcgctgc ggcgagctga tccacgccgt ccattacagc gactacacgc
      301 tctgctactg cccgcgggag cagaccggcg gcgagccgct caaggaccgt cgcctctccc
      361 ggctcctcaa ataagcgggg cgggtcgagc cggcttctag ccgtcgtggt cggagtccgg
      421 ttcctcggaa ctgaggatct cgtcggcttc ggaatagacc gagcccgcgt cctccgcttc
      481 gacaatcgca tcgagttcgg ggtcgcgacc gaggtccgga tcctcggact gatgaatcag
      541 ctccgcttcg gcttcctcga agccttcgga ctcaccttcg ccggattcgc gcaaaggccg
      601 ctcggcctcg ttctcgaatt ccagcctttg ctcaagatca cgccagggac cggtcatggc
      661 gacaagatac tggccgggca atactgctgg accgtccctc cgcccgtcga tcgctacctg
      721 ccgatcgcgg ccggaacccg gttgatccgg aattgctcga gccggcgccg ctggctttct
      781 gcccgctcga ccaggcgatc gaagtcgatt ccttccagac ggggatcctc cagcctggcg
      841 agggccttcc agagcgagag cttcccggtc acgccgatca ccagcgcctc aagctccagc
      901 atctggccga gttcggagtt tccggtcagg cggccattca acttcagccg gccgagtttt
      961 tcggccgtcc aggcgagggc gtacttggcg cggttcggtt tcaccgcgat caggtcacgg
     1021 acatgggtca gggtcacgcg gtcttcctcc acctcgtgcg cgatcgactt ccacatcgcc
     1081 ttgcgctcct ggtcggtggc gttggcggcc gctcgccgcg ccagagccac acccccggtg
     1141 gcgccggtca ggtggtcgtt caggtagatg cccagttcgc gatccgacat ctttctctgg
     1201 taccgcgaca ccccgaaatc aaaccgggac cgggtccgcc atctccccgg tcaggaagaa
     1261 aggcg
//
LOCUS       DAOQMF010000668         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_94433_length_1264_cov_3.507858, whole genome shotgun sequence.
ACCESSION   DAOQMF010000668 DAOQMF010000000
VERSION     DAOQMF010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94433_length_1264_cov_3.507858"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLV77_10085"
     CDS             <1..605
                     /locus_tag="PLV77_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC16240.1"
                     /translation="FGAPGLQAANFLSLFGALDRLGGFFVLASIREFAPFVTAVVVAG
                     VAGTAITADLGARKIREELDALQVLGVDPIKNLVVPRFLALMLITGMLDIYALLFGIA
                     GGIGAELLYSQPLGGFFASLFSNASTTELAASIIKCTLFGAIIAIVCCYKGMTATGGA
                     AGVGRAVNEAVVTALLGVFIFNYLFTQTMLATNPDIQTIK"
     gene            605..>1264
                     /locus_tag="PLV77_10090"
     CDS             605..>1264
                     /locus_tag="PLV77_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC16241.1"
                     /translation="MATSWLQVPREWLESMGEIVRFCGAVMGLVFSGRVFKYFGEVLN
                     QAGILILGSTTVIWGLVFVLGLQCGIEGAYFNRSVGAPAYAGVFAAWCDLREVIPYAF
                     GYMMSAKVGTGIVAELGAMRISDEIDALEVMGVPPVPFLAATGQLRRQSLGHGDIAEV
                     GVAAGIAVDPGLDPRVAPAVVLGGDRLGCAEDRRNAAGRGLTHLDDRPLLAGALFLPE
                     DR"
BASE COUNT          176 a          452 c          389 g          247 t
ORIGIN      
        1 gcttcggcgc cccggggctc caagcagcca acttcctttc gctcttcgga gctctcgacc
       61 gcctcggcgg tttcttcgtt ctggcctcga ttcgcgagtt cgcaccgttc gtgaccgccg
      121 tcgtggtcgc cggtgtggcc ggcaccgcga tcaccgccga cctcggtgcc cgcaagatcc
      181 gcgaggaact cgacgccctc caggtgctcg gcgtcgatcc gatcaagaac ctcgtcgtgc
      241 cccgcttcct cgccctgatg ctgatcaccg gcatgcttga catctacgcc ctgctcttcg
      301 gcatcgccgg cggcatcggc gccgagctgc tctactcgca gccgctgggc ggcttcttcg
      361 cttccctctt ctcgaacgcc tcgaccacag agctcgccgc ctcgatcatc aagtgcaccc
      421 tgttcggcgc aatcatcgcg atcgtctgct gctacaaggg aatgaccgcg accggcggtg
      481 cggccggcgt cggccgggcc gtcaacgagg cggttgtcac cgccctgctg ggggtattca
      541 tcttcaacta cctgttcacc cagaccatgc tggcgaccaa ccccgacatc cagacgatca
      601 agtaatggct acgagctggc tacaggtccc ccgcgagtgg ctcgaatcaa tgggcgagat
      661 cgtccgtttc tgcggcgcgg tcatggggct ggtcttctcc ggccgggtct tcaagtactt
      721 cggcgaggtc ctcaaccagg ccggcatcct gatcctcgga tcgaccacgg tcatctgggg
      781 cctggtcttc gtgctcggcc tgcaatgcgg aatcgagggc gcctacttca accgctcggt
      841 cggcgcaccc gcctacgccg gcgtcttcgc cgcctggtgc gacctgcgtg aggtcatccc
      901 ctacgccttc ggctacatga tgtcggccaa ggtcggcacc ggcatcgtgg ccgagctcgg
      961 cgcgatgcgg atctcggatg agatcgacgc cctcgaagtc atgggcgtac cgccggtccc
     1021 cttcctcgcc gccaccggcc agctgcgacg acagtccctc gggcacgggg acatagcgga
     1081 ggtaggtgtc gctgccggga tcgccgtcga tccgggcctc gacccacggg tagcgccagc
     1141 ggttgttctc ggtggggatc ggctgggctg cgccgaggat cgccgtaacg cggccgggcg
     1201 agggttgacc catctcgatg accgcccact tctcgccggt gccctcttcc ttccagagga
     1261 tcgg
//
LOCUS       DAOQMF010000669         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_94885_length_1261_cov_3.411277, whole genome shotgun sequence.
ACCESSION   DAOQMF010000669 DAOQMF010000000
VERSION     DAOQMF010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94885_length_1261_cov_3.411277"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..771
                     /locus_tag="PLV77_10095"
     CDS             394..771
                     /locus_tag="PLV77_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16242.1"
                     /translation="MTVGSLIDMTPNMRGFRWIAVPHFETVLSRGLGDKTLERAKEEA
                     AQTTDPLVIQGVEHAHRKDVLRVLRAAIMGRIGRAPIIFAELSPDLRELAARGEAGVA
                     LAKEIFEVLQVETGEERVRRLLS"
     gene            768..>1261
                     /locus_tag="PLV77_10100"
     CDS             768..>1261
                     /locus_tag="PLV77_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNC16243.1"
                     /translation="MNLIPAHDLDAETNLLGAMLVYVPAVEWAIEEIALDPGDFYLDR
                     HQAIYAGMLELHLHGRSLDSITVTDELRRQGKLDEAGGQEYVFGLAEKVEAAGNAASF
                     ARIVSEKATLRRMLATSYEIQEVIDAPGLSLEERIDQAEKAPNRIRADRPGEGRPTAS
                     GDSV"
BASE COUNT          250 a          354 c          415 g          242 t
ORIGIN      
        1 agtgccaaac ccggacaggc atcctgccaa acccggacag gcatcctgcc aaacccggac
       61 accggtactg ccaaaccggg acggggccct gttcttaagg gaactgagat cagttcggag
      121 atccttcctg acctcttgca ggagagagaa cgggacctgg ccgggggacc tgtcgattct
      181 cttccctcgg ttgaaatctc agggaacagg aagatctttt tccagagggc gggggcaccg
      241 gcccaggtgg ctggggcaac cctgactgac ctggctggtt ctgatccggt gaaggctgcc
      301 gcgttgaaat caatcaccgt tgatcttgct ggtggtgatg tgcccgctgt cgcgttcttt
      361 ggggcggcgg cggcaagtca gctgctcgcc gcaatgaccg ttggcagtct gatcgacatg
      421 accccgaaca tgcgcgggtt ccggtggatt gcggttccgc atttcgaaac ggtgctcagc
      481 cgaggcctgg gtgacaagac tctcgagagg gcaaaggaag aggccgctca gacaaccgac
      541 ccgttggtga ttcagggtgt tgagcacgct caccgcaagg atgtgttgag ggtgcttcgc
      601 gcggcgatca tgggcaggat cggaagggcc ccgatcattt tcgcggagct ttcccctgac
      661 cttcgtgaac tggcggcaag gggggaggca ggggtcgccc ttgccaaaga gattttcgag
      721 gtgcttcagg tcgagaccgg tgaggaacgg gtcaggcggc ttttgtcatg aacctcatcc
      781 cggcccatga tctcgacgcg gaaacgaacc tgctcggggc gatgctcgtt tatgtgccgg
      841 cagttgaatg ggcgatcgag gaaatcgccc ttgacccggg tgatttctac ctggaccgcc
      901 accaggcgat ttacgcggga atgctcgagc ttcaccttca cggacgatcg ctcgattcga
      961 tcacggtcac cgatgagctt cgccggcaag gcaaactcga tgaggccggc gggcaggagt
     1021 atgtgttcgg cctggccgag aaggttgagg ctgccgggaa cgccgcctcg tttgcccgaa
     1081 tcgtttccga gaaggcaacg cttcgccgga tgctggccac ctcctacgaa atacaggaag
     1141 tgatcgatgc gccaggtctt tcgctcgagg agcgaatcga ccaagccgag aaagcaccga
     1201 accggatccg ggcagaccgg ccgggggagg gcagacccac cgcttccgga gattcggtta
     1261 t
//
LOCUS       DAOQMF010000670         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_96388_length_1251_cov_2.790970, whole genome shotgun sequence.
ACCESSION   DAOQMF010000670 DAOQMF010000000
VERSION     DAOQMF010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96388_length_1251_cov_2.790970"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLV77_10105"
     CDS             <1..391
                     /locus_tag="PLV77_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16244.1"
                     /translation="VNLVLESLDADLQTLTLRLENAIIAEKASRKARKKDLRAELERI
                     LRIGPDATDEQRQRRVALVSTLLSKVVVHDTSDGFDIELFGHLIPTGRRMTTADFAEH
                     VESAASNSREPSTKRKVWLNFNDESIA"
     gene            358..819
                     /locus_tag="PLV77_10110"
     CDS             358..819
                     /locus_tag="PLV77_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16245.1"
                     /translation="MAQFQRRIHRLTSPRPVTVYSIVPKGTLAEKKYQLLSDKAAASD
                     LALDGQLVEEEDEPIDWSKQIKELKAMGLAVDADDTIDEDDIKALWESAEGPYEHLLP
                     PAIPASWKTPETEPDEPAPDPVEAIPEKEFVPFAFSTLEEDEDNGQLGLFD"
     gene            929..>1251
                     /locus_tag="PLV77_10115"
     CDS             929..>1251
                     /locus_tag="PLV77_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16246.1"
                     /translation="MSQTIEIEIAAPDQMLQAAAATRNNLNLRLLTRSDRERAWAIWE
                     TEIAKGENPTEALDTVTSEIEDWLECRRTPACPCCGSRNLIELRDAVAYMKVRSVRVL
                     GSHPIL"
BASE COUNT          322 a          386 c          352 g          191 t
ORIGIN      
        1 ggtgaatctg gtcctcgagt ctctggacgc tgatctccaa accctgacac tccggcttga
       61 gaacgcgatt atcgccgaga aggcatcgag aaaagcaaga aagaaagatc ttcgcgcaga
      121 gctggagcgt attctccgga tagggcccga cgcgaccgat gaacagcgtc aacgaagggt
      181 ggcactcgtc agcactctcc tgagcaaggt cgtggtgcac gacacctcgg acggattcga
      241 catcgaactg ttcggacact tgatcccgac aggtcgccgg atgacaacgg ctgacttcgc
      301 cgagcacgtt gagtcggcag cgtcaaacag tcgcgaaccg tccactaaga ggaaagtttg
      361 gctcaatttc aacgacgaat ccatcgcctg acttcgccgc ggccagtcac cgtctactcg
      421 atcgttccga aaggaaccct cgccgaaaag aagtatcagc tgctgtcgga caaggccgca
      481 gcatccgatc tggcccttga cggccaactg gtcgaggaag aagacgaacc gatcgactgg
      541 agcaagcaga tcaaggaact caaggcaatg ggccttgccg ttgacgccga cgacacgatc
      601 gacgaagacg acataaaagc cctttgggaa tccgccgaag gcccctacga gcatctgctg
      661 cccccggcaa tcccggccag ctggaaaacc ccggaaaccg aaccggacga accggcaccg
      721 gatcccgtcg aggcgatccc tgaaaaggag ttcgtgccgt tcgcgttctc aacactcgaa
      781 gaggacgaag acaacgggca gctcggtctc ttcgactgag ccacccaaca aaccagaagc
      841 actcgagaaa ggccggtccc gatgaaaggg gccggccttt tcttcatgcg cggaaagccc
      901 gcgtatccaa ccaatcaagg agataccaat gagtcaaacc atcgaaatcg aaatcgccgc
      961 cccagatcag atgctccagg cggcggccgc aacccgaaat aacctcaact tgcggctgct
     1021 caccagaagc gaccgcgaac gggcctgggc gatctgggaa acagagatcg ccaagggcga
     1081 gaacccgacc gaggcgctcg acacggtcac cagcgaaatc gaggactggc tggaatgccg
     1141 ccgaacccca gcctgcccat gctgcggatc gagaaacctg atcgagcttc gtgacgcggt
     1201 cgcctacatg aaagtgcgat cggtccgggt gcttggcagc cacccgatcc t
//
LOCUS       DAOQMF010000671         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_96522_length_1250_cov_3.533891, whole genome shotgun sequence.
ACCESSION   DAOQMF010000671 DAOQMF010000000
VERSION     DAOQMF010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96522_length_1250_cov_3.533891"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1250
                     /locus_tag="PLV77_10120"
     CDS             <1..>1250
                     /locus_tag="PLV77_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNC16247.1"
                     /translation="VIAMSMFEALQFEYWQWASLVLAAPVVVWAAWPFHRATLMNLRH
                     GATTMDTLVTIGISAAFFWSLYALFFGTAGEPGMTHPFEITVAPSDGASNIYLEVAAG
                     VTMFLIAGRYFELRSKRRASSAMNALLEMGAKEAFVIRDGEEVAVAIGDLAVGDEFVV
                     RPGEKVATDGVVVSGNSAIDESMLTGEPVPKEVGAGDEVAGATVNAGGRLTVRATRVG
                     ADTQLARMAKMVEDAQSGKAPVQRLADRISAYFVPAVILIAAATLGAWLGAGFPADAA
                     FTAAVAVLIIACPCALGLATPTALLVGTGRGARMGILIKGPEVLESTRQIDAVVLDKT
                     GTVTEGRMELVEVITAEGVDRRELLAIAGALENSSEHPIAKAIARVAAEETGKLPQPE
                     GFASVAGRGVQGVVDGHAVLVGRES"
BASE COUNT          195 a          402 c          450 g          203 t
ORIGIN      
        1 gtgatcgcga tgtcgatgtt cgaggcgctt cagttcgaat actggcagtg ggcctcgctc
       61 gtcctcgccg cgcccgtcgt cgtctgggcg gcctggccgt ttcaccgggc caccctcatg
      121 aacctgcgcc acggggcgac caccatggac accctggtga cgatcggaat cagcgccgct
      181 ttcttctggt cgctctacgc actcttcttc ggtaccgcgg gcgagccggg catgacccat
      241 cccttcgaga tcaccgtcgc tccttccgac ggcgcctcga acatctacct cgaggtcgcg
      301 gcgggcgtga ccatgttcct gatcgccgga cgctacttcg agctgcgctc gaagcggagg
      361 gcgagctcgg ccatgaacgc cctgctcgag atgggcgcga aggaagcctt cgtgatcagg
      421 gacggggagg aagttgcggt cgcgatcggg gacctggcgg tcggtgacga gttcgtggtt
      481 cgtcccggcg agaaggtggc gacggacggt gtggtggtgt cgggcaactc ggcgatcgac
      541 gagtcgatgc tgaccggcga gcccgtcccg aaagaggttg gcgccggcga tgaagtcgcc
      601 ggagcgaccg taaacgcggg cggccgactg acggtcaggg cgaccagggt cggcgccgac
      661 acccagctgg cccgcatggc gaagatggtc gaggatgccc agtcgggcaa ggcaccggtg
      721 cagcgactgg ccgaccggat ctcggcctac ttcgtgccgg cggtgatcct gatcgcggcg
      781 gctacgctgg gagcctggct cggggccggt ttccccgctg acgccgcgtt cacggcggcg
      841 gtcgcggtgc tgatcatcgc ctgcccctgc gcccttggtc tggccacccc gactgcactt
      901 ctggtcggca ccggccgcgg agcccgcatg ggcatcctga tcaaggggcc ggaggttctc
      961 gaatcgacca ggcagatcga cgccgtcgtc ctggacaaga ccggaaccgt gaccgaagga
     1021 cgaatggaat tggtcgaggt catcacggcc gaaggggtcg accgccgcga actgctggcg
     1081 atcgccggag ccctcgagaa ctcctccgag cacccgatcg cgaaggcgat cgcccgggtg
     1141 gcagctgaag agacgggaaa gctgccgcag ccggagggct tcgccagcgt cgccggacgg
     1201 ggagtccagg gcgtggtcga cggtcacgcc gtgctggtcg gtcgcgaatc
//
LOCUS       DAOQMF010000672         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_97804_length_1242_cov_3.513058, whole genome shotgun sequence.
ACCESSION   DAOQMF010000672 DAOQMF010000000
VERSION     DAOQMF010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97804_length_1242_cov_3.513058"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..846)
                     /gene="thiD"
                     /locus_tag="PLV77_10125"
     CDS             complement(61..846)
                     /gene="thiD"
                     /locus_tag="PLV77_10125"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNC16248.1"
                     /translation="MSEERQIPAVLSIAGSDSGGGAGIQADLKAFAACGVHGMTAITA
                     ITAQNTVGVDLIEAISPEMIIAQIKAVAGDIGVDAVKIGMLGDRPTIEAVASGLDLLG
                     DVPVVLDPVMVSESGANLLAVEAREALVTRILPRATVTTPNIPEARAITGLAEAGQPE
                     LAEVIVGLGVKAAVVTGGHSDEYVDFFFDGERGVEITGEVHPGRSAHGSGCTHSSALA
                     AGLALGLGPLEAARKARELASRAVAGSLDGIGSGPGPVNALGL"
     gene            complement(839..>1242)
                     /locus_tag="PLV77_10130"
     CDS             complement(839..>1242)
                     /locus_tag="PLV77_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNC16249.1"
                     /translation="SGSLITAEMASDAGRDVGAVPGPVTAGAAAGTNELLANGAALIR
                     GGQDVLDRMLGAGAGQPLFGPAIEDFEVELLEAVEAGCRTVDEAALMLEREVGEVATA
                     LARLEVRGYLRASTIGTWTRTSLATYPSGGE"
BASE COUNT          198 a          440 c          396 g          208 t
ORIGIN      
        1 cttgaagtta ttcgtcacat gtgcggataa cttcaaccca ggctcctcag gctggttgtt
       61 tcacaggccg agtgcgttga ccggaccggg gccggaaccg atcccgtcca ggcttcctgc
      121 caccgccctt gaggcgagtt cgcgggcctt gcgagcggct tcgaggggac cgagccccag
      181 cgcgaggccg gcggcgaggg ccgaggaatg ggtgcaaccg gagccatggg cggagcggcc
      241 gggatgaacc tcgccggtga tctccacccc gcgttccccg tcgaagaaga aatcgacgta
      301 ctcgtcactg tgaccgccgg tgaccaccgc cgccttgact ccgagaccca cgatcacctc
      361 ggcaagttcg ggctggccgg cttcggccag tcccgtgatg gcgcgtgctt ccgggatgtt
      421 gggagtcgtc acggtggccc gcggcaggat ccgggtgacc agtgcttccc gggcctcgac
      481 cgccagcagg ttcgctccgc tttcggagac catcaccgga tcgagcacga ccggaacgtc
      541 gccaagcagg tcgagaccgg aggccaccgc ttcgatggtc ggccggtccc ccagcatgcc
      601 gatcttcacg gcatcgaccc cgatgtcgcc ggccacggcc ttgatctggg cgatgatcat
      661 ttcggggctg atcgcctcga tcaagtcgac gccgaccgtg ttctgggcgg tgatcgcggt
      721 gatcgcggtc atgccatgga ctccgcaggc ggcaaaggcc ttcaggtcgg cctggatacc
      781 ggctccgccc ccggagtccg atccggcgat cgaaagcacc gccggaatct gtcgttcttc
      841 actcaccgcc cgaaggatag gtggcgagcg aagtccgggt ccaggtcccg atcgttgacg
      901 cccgcaggta gcccctgact tccagccggg ccagggccgt ggccacctcg ccgacctctc
      961 tttccagcat cagggctgct tcatcaacgg ttcgacaacc ggcctccacc gcttccagca
     1021 actcgacttc aaaatcttcg atcgccggac cgaacagggg ctggcctgcc cccgccccga
     1081 gcatccggtc gagcacgtcc tgaccgccac ggatcagggc ggccccgttg gcgagcagtt
     1141 cgttggtccc tgccgcggct ccggcggtca cgggaccggg caccgccccc acgtcccggc
     1201 ccgcatcact ggccatttcg gccgtgatca gcgagccgga ac
//
LOCUS       DAOQMF010000673         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_97826_length_1242_cov_3.177759, whole genome shotgun sequence.
ACCESSION   DAOQMF010000673 DAOQMF010000000
VERSION     DAOQMF010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97826_length_1242_cov_3.177759"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1242
                     /locus_tag="PLV77_10135"
     CDS             <1..>1242
                     /locus_tag="PLV77_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNC16250.1"
                     /translation="GALGVVFGDIGTSPLYAFQTVFQADHHAVKPTDTDVFGVISLVF
                     WSITMIVSVKFVTFIMRADNDGEGGIMALIALVQGASLKNRFVKAGLIGAGLFGVALF
                     YGDGMITPAISVMSAVEGVEVAAPSLSDYVLPMTIGVLVILFAAQRFGTHVIGRAFGP
                     VMVLWFIVIGAAGIGQIAADPEILKALNPAYGVDFFSAHLRIAFISLGAIVLTVTGAE
                     ALYADMGHFGRPPIRRAWFLLVFPALTLNYLGQGSLVLQDPSSVSSPFFLLFPDWSHI
                     PMVVLATIATVIASQAVISGAFSVTKQAIQLGYLPRLLIGQIYMPAVNWALFFAVVIL
                     VVGFGSSAALASAYGIAVVGTLTIDAILFLVVVRKLWQKPLWMAICFGVVFLTIDFTF
                     LAANLTKVEHGGWFPLSIGLI"
BASE COUNT          161 a          426 c          378 g          277 t
ORIGIN      
        1 cggcgcactc ggagtcgtct tcggggacat cggcaccagt ccgctctacg ccttccagac
       61 cgtcttccag gcggaccatc acgcggtcaa gccgaccgac accgatgtct tcggcgtcat
      121 ttccctggtc ttctggtcga tcacgatgat cgtttcggtc aagttcgtga ccttcatcat
      181 gcgggccgac aacgacggcg agggcgggat catggccctg atcgcgctgg tgcagggcgc
      241 cagcctgaag aaccgcttcg tcaaggcggg gctgatcggc gccggcctct tcggggtagc
      301 gctcttttac ggcgacggaa tgatcacccc ggcgatctcg gtcatgtccg cggtcgaggg
      361 ggtcgaggtc gcggcgccgt ctctttccga ttacgtgctg ccgatgacga tcggcgtgct
      421 ggtgatcctc ttcgcagccc agcgtttcgg cacccatgtg atcggccggg ccttcgggcc
      481 ggtgatggtc ctctggttca tcgtgatcgg ggccgccggg atcggccaga tcgctgccga
      541 cccggaaatc ctcaaggccc tcaacccggc ctacggggtc gacttctttt ccgcccacct
      601 gcggatcgcc ttcatctcgc tgggggcgat cgtgctcacg gtcaccggtg ccgaggccct
      661 ttacgccgac atgggccact tcggccggcc cccgatccgc cgggcctggt tcttgctcgt
      721 ctttcccgcc ctgaccctga actacctcgg ccagggatcg ctggtcctgc aggacccctc
      781 ctcggtttcc agcccgttct ttctgctctt ccccgactgg tcgcacatcc cgatggtcgt
      841 cctcgccacg atcgccacgg tgatcgcctc gcaggcggtg atctccggtg ctttctcggt
      901 gaccaagcag gcgatccagc tggggtacct gccgcgcctc ttgatcggcc agatctacat
      961 gccggccgtc aactgggcgc tgttcttcgc ggtggtgatt ctggtggtcg gtttcggctc
     1021 ctcggccgcg ctggcctccg cctacgggat cgccgtggtc ggcaccctga cgatcgacgc
     1081 gatcctcttc ctggtcgtgg tcaggaagct ctggcagaaa ccgctctgga tggcgatctg
     1141 tttcggggtc gttttcctga ccatcgactt caccttcctc gctgcgaacc tgaccaaggt
     1201 cgaacatggc ggctggttcc cgctctcgat cggcctgatc at
//
LOCUS       DAOQMF010000674         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_98481_length_1238_cov_2.862215, whole genome shotgun sequence.
ACCESSION   DAOQMF010000674 DAOQMF010000000
VERSION     DAOQMF010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98481_length_1238_cov_2.862215"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLV77_10140"
     CDS             complement(<1..936)
                     /locus_tag="PLV77_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNC16251.1"
                     /translation="MRVQTPGRGPLFPDQGPLGNRVKRRLIGGTAEIAGFILLTVLSP
                     LLMLVGLIVDLALWLKNRRPFVNIRLIPAVWWFLFGEMKAYLKLIYIYVTTGGPWGKG
                     SISRRRQVYQLRIAWARNHLLAIKFLFGLKFELEGMENTGPGPYVLMPRHVSILDNLI
                     GDVFIGHPQRIGVRYVIKEEIRALTPIDIGGRWIPTVFVKRGSLDPDTEIAAVRALTH
                     EMSPGEIMAIYPEGTRPTHKKIRRAQEVIAEKQPGIAPLAARLNHLLPPRLGGPLAIL
                     DEAAAGIDIVFCGHVGFEGLRTVGDVMAGVLVGRQI"
     gene            1037..>1238
                     /locus_tag="PLV77_10145"
     CDS             1037..>1238
                     /locus_tag="PLV77_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNC16252.1"
                     /translation="MVIAIDGPAGAGKSTVARGLAAELGFTYLDSGAMYRCVALTCLE
                     WGADPTASGEATSTAESIEIGFD"
BASE COUNT          198 a          391 c          434 g          215 t
ORIGIN      
        1 gatctggcgg ccaaccagca ccccggccat cacgtcgccg accgtgcgca ggccttcgaa
       61 gccgacgtgg ccgcagaaga cgatgtcgat gccggcggcg gcctcgtcga ggatcgccag
      121 cggcccaccc aggcgcggag gcaggaggtg gttgagccgg gccgccagcg gggcgatccc
      181 gggttgcttc tcggcgatca cttcctgggc ccgcctgatc ttcttgtggg tagggcgggt
      241 cccctcggga tagatcgcca tgatctcgcc cgggctcatc tcatgggtca gcgccctcac
      301 tgcggcgatc tcggtgtcgg ggtcaagcga gccgcgcttg acgaacacgg tcgggatcca
      361 gcggccgccg atgtcgatcg gggtcagcgc ccggatctcc tccttgatca cgtagcggac
      421 cccgattctt tgcgggtgac cgatgaagac gtccccgatc aggttgtcga ggatgctgac
      481 gtgccggggc atcaggacat atggccccgg tccggtgttc tccatgcctt cgagctcgaa
      541 cttcaggccg aacaggaact tgatcgcgag caggtggttg cgggcccagg cgatccttag
      601 ctggtagacc tgccggcgcc gggaaatcga ccccttgccc caggggccgc cggtcgtgac
      661 gtagatgtag atcagcttca ggtaggcctt catctccccg aacaggaacc accagacggc
      721 ggggatcagg cggatgttca cgaaggggcg gcggttcttc agccagagag cgaggtcgac
      781 gatgaggccg accagcatca gaagcggcga gagcacggtc agcaggatga atcccgcgat
      841 ttcggcggtg ccgccgatca ggcgccgttt gacgcggttg ccgagcggtc cctggtcggg
      901 aaagagtggg ccccggccgg gggtctggac gcgcatcggc tgatcgtatt cgggccggtg
      961 ctctttccgc gccccgccgg gttacccgca ggtctcccgg cccgtaggat gggcggtcga
     1021 tgaaaggcag attcacatgg tgatcgcgat cgacggtccg gccggggccg gaaagtcaac
     1081 cgttgcccgc ggccttgccg cggagcttgg tttcacctac ctcgattccg gggcgatgta
     1141 ccgctgcgtg gccctgacct gtctcgaatg gggtgccgac ccgaccgcct cgggggaggc
     1201 aacttccacc gcggagtcga tcgaaatcgg cttcgatg
//
LOCUS       DAOQMF010000675         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_98766_length_1236_cov_3.638442, whole genome shotgun sequence.
ACCESSION   DAOQMF010000675 DAOQMF010000000
VERSION     DAOQMF010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98766_length_1236_cov_3.638442"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLV77_10150"
     CDS             complement(<1..203)
                     /locus_tag="PLV77_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16253.1"
                     /translation="MAAAEVLHPLGEQLSPRSSKPQNLVGWLFGGTGLALFALMALVG
                     LVMRLIQAETIDITSVWFYRLMTL"
     gene            complement(209..739)
                     /locus_tag="PLV77_10155"
     CDS             complement(209..739)
                     /locus_tag="PLV77_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16254.1"
                     /translation="MEVTFGGFQIAVLAAFLFVALSLVAIFAVVARQARRNVEFAEVT
                     DTGYRLRRYWFAFLCVLLGSGVVLSLFFLPYPDNAKSGATVNVSGGLFYWSMSPDTFK
                     AGSDVTFDVTSSDVNHGLGVYDPDGVLVGSVQAMPGYQNQLDLTLDEPGTYMLACLEY
                     CGYKHHEMMREFEVTP"
     gene            complement(924..>1236)
                     /locus_tag="PLV77_10160"
     CDS             complement(924..>1236)
                     /locus_tag="PLV77_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16255.1"
                     /translation="EAPAQTARAIENFTRTITRSESAASGKAAVAQARKEAAESKKES
                     GRKSNRQSDQAQQNAGNKSGQAKQKAAQLKRQASEKARKARQEALQAQKEAAGSKVES
                     P"
BASE COUNT          224 a          378 c          395 g          239 t
ORIGIN      
        1 agggtcatca gccgatagaa ccagacggat gtaatgtcga tcgtctcggc ctggatcagc
       61 ctcatgacga gtccgaccag ggccatcagc gcgaacagcg ccaacccggt tccaccgaag
      121 agccagccga cgagattctg gggtttcgaa gacctcggac tcagttgctc tccgagtgga
      181 tggagcactt cagccgcggc catctcgttc acggagtgac ctcgaactcc cgcatcatct
      241 cgtgatgttt gtagccgcag tattcgaggc aggccagcat gtaggtgccg ggctcgtcga
      301 gggtcaggtc aagctggttc tggtaacccg gcatggcctg gacgctgccg accaggaccc
      361 catcggggtc atagacaccg agcccgtggt ttacgtcgct cgaggtaacg tcgaaagtga
      421 catcgctgcc ggccttgaag gtgtccggac tcatcgacca gtagaagagt cccccgctca
      481 cgttgacggt cgcgccgctc ttggcgttgt ccggataggg caggaagaag agggagagga
      541 ctacgcccga gccgaggagc acgcaaagga aggcaaacca gtagcggcga agccggtatc
      601 cggtgtccgt gacctccgcg aattcgacgt ttcgccgggc ctgccgggcg accaccgcga
      661 aaatggcgac cagagacagg gccacgaaaa gaaacgcggc aagcactgca atctgaaaac
      721 ccccgaacgt gacttccatg ttcgctcctt tagctcaggc agggaaatcc ggcttgaacg
      781 gcgcgaacct taccggctga aaagcagcga aaatcagtag tttcccgcat gatttccggc
      841 ttcgtggccg gaaagcagcg gttgacgacc gtcgaggccg gcgtaggcgg aaacggggag
      901 gagcgccggc gaaagccgga cggtcagggt gactcgacct tggacccggc tgcttccttc
      961 tgggcctgca gggcttcctg ccttgccttc cgggccttct cgctggcctg ccgcttgagc
     1021 tgcgcggcct tctgctttgc ctgtccggac ttgttcccgg cgttctgttg ggcctggtcg
     1081 gattgccggt ttgacttccg tccggattcc ttcttcgact cggcggcttc cttcctggcc
     1141 tgggcgaccg ccgccttccc ggacgctgcc gattcgctcc tggtgatggt ccgggtgaag
     1201 ttctcgattg ccctggcggt ctgggccggg gcctcg
//
LOCUS       DAOQMF010000676         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_99226_length_1233_cov_3.342954, whole genome shotgun sequence.
ACCESSION   DAOQMF010000676 DAOQMF010000000
VERSION     DAOQMF010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99226_length_1233_cov_3.342954"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..>1233)
                     /locus_tag="PLV77_10165"
     CDS             complement(20..>1233)
                     /locus_tag="PLV77_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC16256.1"
                     /translation="LLYARGDGASSFASPVELLRTGTDEYLNWVSKADLNGDQYDDIV
                     FKVQTATGENIVRAALGSADGPVIQAGEGSSISVYGAQQPAPGDFNGDGDQDVAIPQF
                     VDDGFQVGLGDGSGRLALDFAGPFALAAVDSADFFPQASATLDANGDGRLDFAAASGH
                     SGDADQARGVAVMLNKPSSGISVSPTSIHFGDVPFDAELIAPVPVTIKSNGDLPLNLG
                     QIGFSGPATGGFSLDRGNCPNGNLASGSTCTLEVSMKQNQSGYFNGFLDITSDATGDP
                     VRIEVIGQVGEAPPTARGLSLKVKSAKKVKAGKKLVVSAIVRNSGKARSAHFTIRATA
                     PKKMTGRIKFSPLRNLSLDGRSYTTFFFKVPVKKQAKGVLRVKVSLVAKNRKIVRKTG
                     KVRIVKKARRR"
BASE COUNT          200 a          390 c          402 g          241 t
ORIGIN      
        1 ataaacgcgg gcgaaggtct taccgccgcc gcgccttctt cacgatcctg accttgcctg
       61 tctttcgcac gatcttccgg ttctttgcca ccaggctcac cttgacccgg agcacgccct
      121 tcgcctgctt cttgacggga accttgaaga agaaggtcgt gtagctgcga ccgtcgagcg
      181 agagattgcg aagcggcgag aacttgatcc ttccggtcat cttcttcggt gcggtggccc
      241 tgatcgtgaa atgcgccgag cgggccttgc cgctgttgcg gacgatcgcg gacacgacca
      301 gcttctttcc ggccttgacc ttcttcgccg acttcacctt cagggaaagc cccctggcag
      361 tgggcggggc ttccccgacc tggccgatca cctcgatccg gaccggatcg ccggtcgcgt
      421 cgctggtgat gtcgaggaag ccgttgaagt agccggactg gttctgcttc atcgagacct
      481 cgagcgtgca ggtcgagccg gacgcgaggt tgccgttcgg gcagttgccg cggtcaaggc
      541 tgaaacctcc ggttgctggg ccgctgaagc cgatctgacc gaggttcagc gggagatcgc
      601 cgttggattt gatcgtgacc ggaaccggtg cgatcagctc ggcgtcaaac gggacatctc
      661 cgaagtggat cgaggtcggt gagacggaga tcccggagga gggcttgttc agcatgaccg
      721 cgacgccgcg agcctgatcc gcatcacccg aatggccgct ggccgccgcg aagtcgagcc
      781 ggccgtcccc gttggcgtcc agggtggcgc ttgcctgggg aaagaagtca gccgagtcga
      841 ctgccgccag ggcgaacggt ccggcgaagt cgagagccag cctgccgctg ccgtcgccga
      901 gaccaacctg gaatccgtca tcgacgaact gggggatcgc tacgtcctgg tcaccgtcac
      961 cgttgaagtc tcccggggcc ggctgctggg cgccgtagac ggaaatcgag ctgccttcgc
     1021 cggcctggat gaccggtccg tcggcgctgc cgagcgcggc cctgacgatg ttttctccgg
     1081 tggcggtctg caccttgaag acgatgtcgt cgtactggtc accgttcaga tcggccttgc
     1141 tcacccagtt caggtattcg tcggttccgg tccgcagcag ttcaaccggc gaggcgaagc
     1201 tcgatgcgcc atcgcccctt gcgtaaagga gcc
//
LOCUS       DAOQMF010000677         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_99435_length_1232_cov_2.335599, whole genome shotgun sequence.
ACCESSION   DAOQMF010000677 DAOQMF010000000
VERSION     DAOQMF010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99435_length_1232_cov_2.335599"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLV77_10170"
     CDS             <1..913
                     /locus_tag="PLV77_10170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNC16257.1"
                     /translation="LTGLPFDIAAWQLGRDYGLVNQTLGDRLLDWLLATVVTAIPAAL
                     GGLLAMALWRRLKGRFWIAASVLVAIWAVITVWLWPVVISPLFNDFDPLPDGPARTGV
                     LRLAERAGVEVGEVYEVDASRRSSTLNAYVNGIGPTKQVVIYDNAIRTLNRGEFTALV
                     GHELGHVKAADLRRGLLFALLVIPLGVLFVQLATAAALRRNGDDLRSPALIPVIALWM
                     ALAAFALQIPGNALSRQVEAKADRYSIALTGDPQGLVDLQVRLAKANLSDVDPPAFWQ
                     FMFGTHPSTFDRIAIAEGAKGGSRDEG"
     gene            903..>1232
                     /locus_tag="PLV77_10175"
     CDS             903..>1232
                     /locus_tag="PLV77_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNC16258.1"
                     /translation="MKVELIAVGKIKPPFAEAEAHYLKLLKPLMPVEVTETRDDDALG
                     RQVKSALATGGNRPDPGTIVALEAGGRDMTSERWAEWLDQRRHDGQRLTLLIGGPEGL
                     PGEVSGMA"
BASE COUNT          186 a          385 c          435 g          226 t
ORIGIN      
        1 attgaccggt ctgccctttg acatcgccgc ctggcaactc gggcgggact acggactggt
       61 caatcagact ctcggtgacc gcttgctcga ctggctgctg gccaccgtgg tcacggcaat
      121 tcctgctgcg ctgggcggac tgctggcgat ggcactttgg cgaaggctga agggcaggtt
      181 ctggatcgcg gcgtcggttc tggtcgcgat ctgggcggtg atcaccgtct ggctctggcc
      241 ggttgtcatc tcgccgttgt tcaacgattt cgacccgctt cccgacggcc cggcgcgaac
      301 cggggtgctt cgactggcgg aacgggccgg ggtggaggtg ggggaggtct acgaggttga
      361 cgcaagtcgc cgttcctcta ccttgaatgc gtacgtgaac ggaatcggcc cgaccaaaca
      421 ggtggtcatc tatgacaacg cgatccggac cctgaaccgg ggcgagttca cggccctggt
      481 cgggcatgag ctcgggcatg tgaaggcggc ggacctgagg cgcgggctgc tcttcgccct
      541 gctggtcatt ccgctggggg tgctcttcgt ccagcttgcc accgcggcgg ccctgcgccg
      601 caatggcgac gacctccgca gtccggcgct gatccctgtg atcgccctct ggatggccct
      661 ggcggctttt gcgctccaga ttcccggcaa cgcgctgtcc cgccaggtcg aggcgaaggc
      721 ggaccgttac tcgatcgccc tgaccggcga tccccagggt ctggtcgacc tccaggtgcg
      781 gctcgccaag gccaacctgt cggatgtcga tccgccggcc ttctggcaat tcatgttcgg
      841 tacccatccc tcgaccttcg atcggatcgc gatcgccgag ggtgcgaagg gaggcagccg
      901 ggatgaaggt tgaactgatc gcggtgggga agatcaagcc cccgtttgcg gaggccgagg
      961 cccattacct gaagctgctg aagccgctga tgccggtcga ggtcaccgaa accagggacg
     1021 acgacgcctt ggggcggcag gtgaagtccg ccctggcgac cggcggaaac cggcccgatc
     1081 cgggcacgat cgtcgcgctt gaggccggcg gacgggacat gacctccgag cgctgggccg
     1141 aatggctgga ccagcgccgc cacgacggtc agaggctgac cttgctgatc ggcggtccgg
     1201 aaggtttgcc gggggaggtc tcggggatgg cc
//
LOCUS       DAOQMF010000678         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_99581_length_1231_cov_2.602891, whole genome shotgun sequence.
ACCESSION   DAOQMF010000678 DAOQMF010000000
VERSION     DAOQMF010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99581_length_1231_cov_2.602891"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLV77_10180"
     CDS             <1..904
                     /locus_tag="PLV77_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC16259.1"
                     /translation="IPILNQADGGSVAMVSPGNTAICLTESADTCTDGTPDSLYPAGD
                     RNYARVVPNDAAQGAGLVTYAAGNGIKRVAVLFAANDDTSYGQAQTFINASGPGGVKV
                     TEQLEWDPEAASYTDLMNKVKKSDPDGILLAGLTEQNGGQLIKDKVAVFGPNDGSVAL
                     MAPDGFAQQSTIDEAGPASAGMIATVPGRAPELLSDSGKQFVKQLESKTGGQPVEIYA
                     PYAGQAAQVLLDAIESAGNDRAGVARALYGIKVTDGITGDFTITDTGDPDVSPITVNK
                     AGKEFEPVEVIEPKANLIKAARGQ"
     gene            complement(1038..>1231)
                     /locus_tag="PLV77_10185"
     CDS             complement(1038..>1231)
                     /locus_tag="PLV77_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HNC16260.1"
                     /translation="TAVLLWKALFDAGVYCNVAIHPAVPPSGALLRTSLMATHEKEQL
                     DRALGIIGEVIKDFPTLTG"
BASE COUNT          229 a          410 c          415 g          177 t
ORIGIN      
        1 gatcccgatc ctcaatcagg ccgatggcgg ctcggtggcg atggtctcgc ccggcaacac
       61 ggcgatctgc ctgaccgaat cggcagacac ctgcaccgac ggcacccccg actccctcta
      121 cccggccggc gatcgcaact acgcgcgggt cgttcccaac gatgccgccc agggggccgg
      181 tctcgttacc tacgcggccg ggaatggcat caagcgggtc gcggtcctgt ttgccgccaa
      241 tgacgacacc tcgtacggac aggcgcagac cttcatcaac gcctccggtc cgggcggcgt
      301 caaggtcacc gagcagctcg agtgggatcc ggaagcagcg agctacaccg atctcatgaa
      361 caaggtgaag aagagcgacc cggacggcat cctgctcgcc ggcctgaccg agcagaacgg
      421 tggtcagctg atcaaggaca aggtcgcggt cttcggtccg aatgacggca gcgtggcgct
      481 gatggcgccg gacgggttcg cccagcagtc gaccatcgac gaggccggcc cggcctccgc
      541 gggcatgatc gccacggttc ccggccgcgc ccccgagctg ctgtcggatt ccggcaagca
      601 gttcgtgaag caactggaat caaagaccgg cgggcagccg gtggagatct acgcccccta
      661 cgccggtcag gcagcccagg tcctgctcga tgcgatcgag tcggccggaa acgaccgggc
      721 cggggttgcc agggcgctct atggcatcaa ggtgaccgat ggcatcacgg gcgacttcac
      781 gatcaccgac accggggatc cggacgtcag cccgatcacg gtcaacaagg ccggcaagga
      841 gttcgagccg gtcgaggtga tcgagccgaa ggcgaacctg atcaaggccg cccggggtca
      901 gtaggaacca gcagaagaac tgatcccggt gtgccggact cctcgacccg aaacgtcgtc
      961 gcccggcaca ccgggatcag tccaatcaag actgtcggtc ctccgggccg cagccccacg
     1021 cgagcggggc gccctggcta accggtgagg gtggggaagt ccttgatgac ttcgccgatg
     1081 atcccgaggg ccctgtcgag ctgctccttc tcgtgggtgg ccatcaggct ggttcgcagc
     1141 agggcgccgc tgggcgggac cgccgggtgg atcgccacgt tgcagtagac gccggcgtca
     1201 aagagggcct tccagagcag gaccgcggtc c
//
LOCUS       DAOQMF010000679         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_99705_length_1230_cov_3.741277, whole genome shotgun sequence.
ACCESSION   DAOQMF010000679 DAOQMF010000000
VERSION     DAOQMF010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99705_length_1230_cov_3.741277"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLV77_10190"
     CDS             <1..418
                     /locus_tag="PLV77_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16261.1"
                     /translation="VIPVLKINKKRQSDVTAVEVDILRGASSVITSGQLAVTLYQAGP
                     VTDKIQPRVLRAGIYTEAGELISDSHDLTFDLSSDNPRERELQVRFVLTRKADEANGQ
                     EVILRLEEKHAGTSHYKEYKSLRYLMRRSFTSDFDF"
     gene            433..>1230
                     /gene="brxL"
                     /locus_tag="PLV77_10195"
     CDS             433..>1230
                     /gene="brxL"
                     /locus_tag="PLV77_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HNC16262.1"
                     /translation="MNELDQKINTHFPGLVVRKDLVKTVKGNAIVPSYVLEYLLGQYC
                     ATSDEPTIQTGIETVKEILAKHYVHRNEAGLVRSNIKEKGRYKVIDKISVDLNDKKDV
                     YEAQFSNLGIKEVLVDSGTVKKHPKLLVGGVWCIADLEYEFTEDKAVSPWVLSTLKPI
                     QLSHFDFDGYVEARKQFTTDEWIDLLVQSIGFNPDMFGKRSKLTQLLRLIPFCERNYN
                     LIELGPKGTGKSHIYSEFSPHGILISGGEVTVPKLFVNNSSGKIGLVG"
BASE COUNT          293 a          353 c          343 g          241 t
ORIGIN      
        1 ggtgatcccg gtgctcaaga tcaacaagaa gcgccagagc gatgtcaccg ccgtcgaggt
       61 ggacattctg cgcggagcca gctcggtgat cacctccggg caactggcgg tgaccctgta
      121 ccaggccggg ccggtgacgg acaagatcca gccgcgtgtg ctgcgggctg gcatctacac
      181 cgaggcaggc gagctgatct ccgacagtca cgacctgacc tttgatctca gctcggacaa
      241 tccaagggag cgggagctgc aggtgcgctt tgtgctgacc cgcaaggccg atgaggccaa
      301 tggccaggag gtcattctac ggctggaaga gaaacatgcc gggacttcac attacaagga
      361 atacaagtcg ctccggtact tgatgcggcg ctcatttacc agcgacttcg acttttaaag
      421 gacgggtgat cgatgaacga acttgaccag aaaatcaaca cacacttccc gggactcgtt
      481 gtccgcaagg atctcgtcaa gacggtcaag ggcaacgcca tcgttccttc ctacgtgctg
      541 gaatacctgc tgggccagta ttgcgccacc agcgacgagc cgaccattca gaccggaatc
      601 gagacggtca aggagatcct tgccaaacat tatgttcacc ggaatgaagc cggattggtc
      661 cgctcgaaca tcaaggaaaa ggggcgctac aaggtcatcg acaaaatcag cgttgacctg
      721 aacgacaaga aggatgtcta cgaggcgcag ttctccaatc tgggcatcaa agaggttctt
      781 gttgattccg gaacggtgaa aaagcacccc aagctcctgg tcggcggagt gtggtgcatt
      841 gctgatctgg aatatgagtt taccgaagac aaagccgtca gcccctgggt cttgtccact
      901 ctcaagccga tccagctctc ccatttcgat ttcgacggct atgtcgaggc ccgcaagcaa
      961 ttcaccaccg acgagtggat cgacctgctg gtgcaaagca tcggcttcaa cccggatatg
     1021 ttcggcaagc gcagcaagct gacccaactg ctccgcctga tcccgttttg cgagcgtaac
     1081 tacaacctga tcgaacttgg ccccaaggga accggcaaat cgcacatcta ctcggagttt
     1141 tcccctcacg gcatcctgat ctccggcggc gaggttacgg ttcccaagct gttcgtgaac
     1201 aactcttcag gaaagatcgg cctggtcggc
//
LOCUS       DAOQMF010000680         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_100198_length_1227_cov_4.483788, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000680 DAOQMF010000000
VERSION     DAOQMF010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100198_length_1227_cov_4.483788"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..>1227)
                     /locus_tag="PLV77_10200"
     CDS             complement(182..>1227)
                     /locus_tag="PLV77_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC16263.1"
                     /translation="WLGIEVCNYHTDSMSRQAEAAIRAAGDQPFYLQVDYHAPHGDSR
                     PPIGPEPAPRHYDTALKTPGPRPRGFNERDITDKPRFLQEADAAAPLTQTEISQLDTE
                     YRKSIEALRSVDDGVGGIIKALRETGRLANTYVIYTSDNGFFTGQHRISRGKLLPYEP
                     ALRVPFVIRGPGIRPGTRSFEPVANQDVAPTIIRLAGARAALTIDGRSMIPFWKDPKK
                     RSRRPILLSSYATITRLIPGDYPDDPEQPGDGGTEDRARRGKDASASVRSPDQNYVGI
                     RLGPYKYIRYESGETELYVLSEDPAELENRAGDPRYLKVINYLETQLADLRGCKGQTC
                     RSWASRWPSPPGA"
BASE COUNT          183 a          388 c          430 g          226 t
ORIGIN      
        1 cctggtcaac ccggcctcga tccaccaggg caagaaccaa atcggcatct accgcctcca
       61 ggacaaccgg ctgataccgc tcggaggaaa taaccagtag agcccctccc aaccattcat
      121 tgcttcgaat gggaggggcg ggttgaaact ggagggcgag cgcagactcg cccgactgca
      181 gttaagcccc cggaggggaa ggccaccggc tagcccagct ccggcaggtc tggcccttgc
      241 agccgcggag atcggcgagc tgggtttcga ggtagttgat gaccttcagg tagcgggggt
      301 cgccggcacg gttttccagt tcggccgggt cctcgctgag tacgtacagt tcggtttcgc
      361 cgctctcgta acggatgtac ttgtaggggc cgagccggat cccgacgtag ttctggtccg
      421 ggctgcggac cgaggcgctc gcgtccttgc cgcgcctggc gcgatcctcc gttcctccat
      481 cgccgggctg ctccggatca tccgggtaat cgccggggat caggcgagtg atcgtcgcgt
      541 agctggaaag caggatcggc cggcgtgacc gcttcttcgg gtccttccag aacggaatca
      601 tcgagcggcc gtcgatcgtc agggcggccc gggcgccggc cagcctgatg atggtcggcg
      661 cgacatcctg gttggccacc ggctcgaagc tcctggtccc cggcctgatt cccgggccgc
      721 ggatcacgaa cggcacccgc agggccggct cgtagggcag cagcttgccg cggctgatcc
      781 ggtgctggcc ggtgaagaag ccgttgtccg aggtgtagat cacgtaggtg ttggccaggc
      841 gcccggtctc ccgcaatgcc ttgatgattc ccccgacccc gtcgtcgacc gaacgcaggg
      901 cctcgatcga cttgcggtac tcggtgtcga gctggctgat ctcggtctgg gtgagcggtg
      961 cggccgcgtc ggcttcctgg aggaaccgcg gcttgtcggt gatgtcccgc tcgttgaagc
     1021 cgcgcggacg tggtccgggt gtcttcaggg cggtgtcgta gtgacggggc gccggttccg
     1081 ggccgatcgg tggccgcgaa tcgccatgcg gggcgtggta gtccacctgg aggtagaagg
     1141 gctggtcacc ggccgcccgg atcgccgctt ctgcctgccg ggacatcgag tccgtgtggt
     1201 agttgcagac ctcgatcccc aaccagg
//
LOCUS       DAOQMF010000681         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_100726_length_1224_cov_3.528657, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000681 DAOQMF010000000
VERSION     DAOQMF010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100726_length_1224_cov_3.528657"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLV77_10205"
     CDS             complement(<1..667)
                     /locus_tag="PLV77_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16264.1"
                     /translation="MDAESNGRPSEEETVEETVASPSLTERLVGAGTGGARRMAGATG
                     LDQAMENAVEEALVRAIESEAVERSMTRIINGPLIENAVRDAMKSPAVEQALMEVVDS
                     ELVDRLWARILDSNETQRLIERIAEAPEVRAAIASQSLGLVDDLGRGAARVTRAIDFA
                     LERLARRIFFRSRREVPTDRVGVATRALALVIDGVAVNLVMISSTAVLGMIASVLGID
                     LGNL"
     gene            complement(698..>1224)
                     /locus_tag="PLV77_10210"
     CDS             complement(698..>1224)
                     /locus_tag="PLV77_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16265.1"
                     /translation="LSGGRAAAFTEGALSLTFTLPDPKPAERTVQRFTEWSGLVRDAF
                     IDLGVDTRIGAVPDEYCPGDFSVNAGGRVKLAGAGQRMIRGAAHLGFVILVSDSALVA
                     ETLGPVYAALGLDFNPGTVGSIEDARPGTGLEDVEQALLGRLESAVGLQPAEIDPVTL
                     EMTRSRSGQFRSNP"
BASE COUNT          187 a          429 c          406 g          202 t
ORIGIN      
        1 cgaggttgcc caggtcgatt cccaggaccg aagcgatcat cccgagcacg gcggtcgagg
       61 agatcatcac caggttgact gcgactccgt cgatcaccag tgcgagggcg cgggttgcga
      121 cgccgacccg atcggtcgga acctcccgcc tcgaccggaa gaagatgcgg cgggcgagcc
      181 gctcgagggc gaagtcgatc gcccgggtga cccgcgcggc gccgcggccg aggtcatcga
      241 ccaagccgag gctctgcgag gcgatcgcgg cccggacctc cggcgcctcc gcgattcgct
      301 cgatcagtcg ctgggtttcg ttgctgtcga ggatccgggc ccagagccgg tcaaccagtt
      361 cggaatcgac cacctccatc aaggcctgct cgactgccgg gctcttcatc gcatcccgca
      421 cggcgttttc gatcaggggc ccgttgatga tccgggtcat cgagcgctcc acggcctcgc
      481 tttcgatcgc ccggacgagc gcctcctcga ccgcgttttc catggcctgg tcgagtccgg
      541 tcgcaccggc catccgcctg gccccgccgg tcccggcccc gaccagccgc tcggtcaggg
      601 agggtgacgc gaccgtctcc tcgactgtct cctcctccga aggccgtccg ttcgattcgg
      661 cgtccatggc ttcagattag aggctgctga ccgggcatca gggattcgag cgaaattgtc
      721 cggatcgtga cctggtcatc tcaagggtca ccggatcaat ttcggccggt tgaagtccga
      781 ccgctgactc gagccgtccg agcagcgcct gctccacgtc ttcgagtccg gttccgggcc
      841 gggcatcctc gatgctgccg accgtccccg ggttgaagtc gagcccgagc gccgcgtaga
      901 ccgggcccag cgtttcggcg accagggccg agtccgagac cagaatgacg aagccgaggt
      961 gagcggcgcc cctgatcatt cgctgtcccg ctccggccag cttgacccgg ccgccggcgt
     1021 tgacgctgaa atcgcccggg cagtattcgt ccggcaccgc accgatcctg gtatcgacgc
     1081 cgaggtcgat gaacgcgtcc cggaccaggc cggaccattc ggtgaagcgc tggacggtcc
     1141 gctcggccgg cttcggatcg ggcagggtga aggtgaggct cagggcaccc tcggtgaagg
     1201 cggcggcccg gccaccggac agcc
//
LOCUS       DAOQMF010000682         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_100866_length_1223_cov_4.903253, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000682 DAOQMF010000000
VERSION     DAOQMF010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100866_length_1223_cov_4.903253"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLV77_10215"
     CDS             <1..301
                     /locus_tag="PLV77_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16266.1"
                     /translation="VQRYIDTDAPTQVEWDGYVEDDQNWIWTMPNGTGVGAATLPWLV
                     IFKTDARPKGTIKCKATVGDKSARVLIPFRQDEWPIADDQPNTDEAGTGDVTNVQ"
     gene            complement(305..1015)
                     /locus_tag="PLV77_10220"
     CDS             complement(305..1015)
                     /locus_tag="PLV77_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNC16267.1"
                     /translation="MCGRYTLTSSDQRRLGSRFQISLDDESPGLGRYNVAPTQEVLTV
                     NTHEGSGEREAVASRWGLVPFWAKDLKAGYKMINARNDRLLQSKAYGPLVKKSKNRCL
                     IVADGFYEWLKAEKPKEPRQPVRFTVDGGEPFAFAGLTVSREWEGEPMTSCTIITTDA
                     NDVVSQVHDRMPVILPNEEVEQAWLTGDLSPEEAVSLCVPLDPKRMEAKPANPVLNKV
                     GGAKEGPDFLEAPEKNSD"
BASE COUNT          220 a          404 c          370 g          229 t
ORIGIN      
        1 cgttcagagg tacatcgaca ccgacgctcc gacccaggtc gagtgggacg gatacgtcga
       61 agacgaccag aactggatct ggacgatgcc gaacggaacc ggggtcggtg ccgcgacgct
      121 gccctggctg gtcatcttca agaccgatgc ccgccccaag ggaacgatca agtgcaaagc
      181 caccgttggt gacaagtcgg cccgggtcct gatccccttc cgtcaggacg aatggccgat
      241 cgccgacgat cagccgaaca ccgacgaggc cggcaccggc gacgtcacca acgtccagta
      301 gcggctagtc ggagttcttt tccggggcct cgaggaagtc ggggccttct tttgccccgc
      361 cgaccttgtt cagcaccggg ttggccggtt tcgcctccat ccgcttcggg tcgagcggca
      421 cgcagagcga aactgcctcc tcgggagaaa ggtcaccggt cagccaggcc tgctcaacct
      481 cttcgttcgg caggatcacc ggcatccggt cgtgaacctg actgaccacg tcattggcgt
      541 cggtggtgat gatcgtgcag gaggtcatcg gctccccttc ccactcccgg gaaaccgtca
      601 aaccggcgaa agcgaaaggc tctcccccgt cgaccgtgaa acggaccggc tggcgcggct
      661 ccttcggctt ctctgccttc agccattcgt agaaaccgtc ggccacgatc aggcagcgat
      721 tcttgctctt cttgaccagc ggaccgtagg ccttgctctg aagcaaacgg tcgttgcggg
      781 cgttgatcat cttgtagccg gccttgaggt ccttggccca gaacggaacc agaccccacc
      841 gcgaggcaac cgcttcgcgc tcgccggacc cttcgtgcgt gttgacggtc agcacttcct
      901 gggtcggcgc cacgttgtag cgcccgagac cgggcgactc gtcatcaagt gaaatctgga
      961 accggcttcc gagccgccgc tgatcggatg aagtcagtgt gtatctgccg cacatggccc
     1021 ccaacctaac agggcggaaa tcgcccgcat gtcaacggcc attcccgtgg ttcctgtacc
     1081 actcgaccgc ttcggtgatt ccggcgccga gggtcatctc gggcttccag ccggtgagtt
     1141 cggtcagttt ggtcgcgtcg acgaactggc ggtcgatttc acccgggggc acgccgtcac
     1201 ccaggaattc gggctcgacc agt
//
LOCUS       DAOQMF010000683         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_100903_length_1223_cov_3.370719, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000683 DAOQMF010000000
VERSION     DAOQMF010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100903_length_1223_cov_3.370719"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLV77_10225"
     CDS             complement(<1..326)
                     /locus_tag="PLV77_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16268.1"
                     /translation="MGKYLAGMLLTVFGLFALAPNAGAAEIPPSEECVATAKKLLGEY
                     PDVENFTEEETRKADRKFVNDLADSGCISDSRPLLQKVVLKPNSEKCVTAARGAGAFW
                     GATTKR"
     gene            complement(337..786)
                     /locus_tag="PLV77_10230"
     CDS             complement(337..786)
                     /locus_tag="PLV77_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16269.1"
                     /translation="MSNRELSLKVTGASLPAPAIRDGSNRGRFLVAAAGFVVYETLFI
                     WLLGDRVYDYPYFSPMLWGWVALAAAGFFALGFFSRSWWSSLLLAAPLLVAFYFVYVV
                     WTTNYPNGGSLTVNTNFPEIWLAYSAIFVPVWAAGVLIAKGRGRGSK"
     gene            complement(832..904)
                     /locus_tag="PLV77_10235"
     tRNA            complement(832..904)
                     /locus_tag="PLV77_10235"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ctt)
     gene            complement(993..>1223)
                     /locus_tag="PLV77_10240"
     CDS             complement(993..>1223)
                     /locus_tag="PLV77_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16270.1"
                     /translation="EKTKQQALDATDDKGDQQQINQTYKSTKKDAQRVGKLIDQAIKQ
                     ARKNGGKSLENPLGSLGSDNSIDSGSGSTATP"
BASE COUNT          221 a          387 c          366 g          249 t
ORIGIN      
        1 aacctcttcg tggttgctcc ccagaacgct ccggcccctc gcgcagccgt tacgcacttc
       61 tcgctgttcg gcttgagcac aaccttctga aggagtggcc tggaatctga aatgcagcct
      121 gaatcggcga gatcgtttac gaacttccgg tcggcctttc tggtctcctc ctcggtgaaa
      181 ttctcgacgt ccggatactc gccgagcagc ttcttggcgg tggcgacgca ctcctcgctc
      241 ggcggaattt cggccgctcc cgcgttcgga gcaagcgcga acagcccgaa aaccgtcaac
      301 agcatccctg ccaggtattt ccccatgcgc ttcattctat ttcgaccctc gaccccggcc
      361 ctttgcaatc aacacccctg cagcccagac cgggacgaag atggccgagt aggcgagcca
      421 gatctcgggg aagttggtgt tgacggtgag gctgcccccg tttgggtagt tcgtggtcca
      481 gacgacgtac acgaagtaga aggcgacgag caacggtgcc gccagaagga gcgaggacca
      541 ccacgacctc gagaagaaac ccagggcgaa gaaaccggct gcggcgagag cgacccagcc
      601 ccaaagcatg gggctgaagt acgggtagtc atagacccgg tcgccgagga gccagatgaa
      661 gagcgtctcg taaaccacga agccggcggc cgcgaccagg aagcggcctc gattgctgcc
      721 gtcccggatt gccggtgccg gaagcgacgc ccccgtgacc ttcaagctga gttccctgtt
      781 actcactgtc atgcctcctg ccgacatatc ccctgtcgaa tcgtttccct atgggccgtg
      841 tagggatcga acctacgacc ttgagattaa gagtctcctg ctctaccaac tgagctaacg
      901 gcccggggtg cggcttcctt gaaaagccgg acgcggatta tagagcgggc gagccgattc
      961 tgtggcccgc ccgctctcag gtcccgggtg gatcagggag tggcggtgct gcccgagccg
     1021 ctatcgatcg agttgtcgga gccgaggctg ccgagcgggt tctcgagcga cttgccgccg
     1081 ttcttccggg cctgcttgat cgcctggtca attagcttgc cgacccgctg cgcgtccttc
     1141 ttggtcgact tgtaagtctg gttgatctgc tgctggtcgc ccttgtcatc ggtcgcgtcg
     1201 agagcctgct gcttcgtctt ctc
//
LOCUS       DAOQMF010000684         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_101123_length_1222_cov_2.787489, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000684 DAOQMF010000000
VERSION     DAOQMF010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101123_length_1222_cov_2.787489"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1222)
                     /locus_tag="PLV77_10245"
     CDS             complement(<1..>1222)
                     /locus_tag="PLV77_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNC16271.1"
                     /translation="VELSLVAVGGVVSVVAVALLSRRVGIAAPLLLVLAGAAIGALPR
                     VSATDIEPQWILGGVLPPLLYSAAINMPTTDFRRNLKPITGLAVLLVVVSTGIAGVLF
                     HTLMPEIGWPAAFALAAVLSPTDAVAATAVGRRLGLPSRLLVILEGEGLVNDVTALVL
                     LRSAVAAIGAGVTVDGVIGDFIVAVVGAIGIGLLVGIVNVRIRQRISDPVMNTAISFV
                     VPFLAYIPAEDFGFSGIFAVVVCGLVTSDLAARYLRAEDRLTEETNWQTLAFLMEGFI
                     FLLMGISLEDVLSYAGNHGFSIEQAVLYGLIAAVLLIVVRVMFVVPLVAMIKRDDQRA
                     ADAKPRVEDFKQKLERDELPDDMLRHGTERVEHRIAAVSADIEFRISQSLGWRAGAVL
                     AWSGMRGAVTVAAA"
BASE COUNT          238 a          414 c          405 g          165 t
ORIGIN      
        1 tgggcggccg ccacggtcac cgcgccgcgc atgcccgacc aggcgagaac ggcgccggcc
       61 cgccagccga gcgactgaga gatgcggaac tcgatgtcgg ccgagacggc ggcgatccgg
      121 tgctcgaccc tttcggttcc atgccggagc atgtcgtccg gcagttcgtc gcgttcaagc
      181 ttctgtttga agtcctcaac ccgtggtttt gcgtccgcgg cccgttggtc gtctcgtttg
      241 atcatcgcca ccaggggcac gacgaacatg acccggacca cgatcaggag caccgcggcg
      301 atcaacccgt agagaacggc ctgctcaatg ctgaacccgt ggttgcccgc gtaggagaga
      361 acgtcctcga ggctgatccc catcagcagg aagatgaagc cctccatcag gaaggccagc
      421 gtctgccagt tggtctcttc agtcaaacgg tcttccgccc gcaggtaccg cgcggccagg
      481 tcgctggtca cgaggccgca gaccaccacc gcgaagatgc ccgaaaagcc gaagtcctcg
      541 gccgggatgt aagcgaggaa ggggacgacg aaggagatcg ccgtattcat caccgggtcc
      601 gagatccgtt gccggatccg cacgttgacg atcccgacca gcaggccgat gccgatcgca
      661 ccgaccacgg ccacgatgaa gtctccaatc acaccgtcga ccgtcacccc ggcgccgatc
      721 gcggcgacgg cgctgcgcag aagaaccagg gcggttacgt cattgaccag cccctcgcct
      781 tcgaggatga ccagaagccg ggagggcagg ccgaggcgcc ggccgaccgc cgtggcggcc
      841 accgcatcgg tcgggctcag gaccgcggcc agggcgaaag ctgccggcca gccgatttcc
      901 ggcatcaggg tgtggaacag aaccccggcg atcccggtcg agacgacgac cagcaacacg
      961 gccaacccgg tgatcggttt caggttccgc cggaagtcgg tggtcggcat gttgatcgcc
     1021 gccgagtaga gcaacggcgg caggactccg ccgaggatcc actgcggctc gatgtcggtc
     1081 gcagaaaccc gggggagggc tccgatggcg gctccggcca gcaccagcag aagcggtgcg
     1141 gcgatcccga cacggcggga aagcagcgcc accgcgacca cgctgacgac accgccgact
     1201 gcgaccaggg agagctccac gg
//
LOCUS       DAOQMF010000685         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_102310_length_1215_cov_2.570690, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000685 DAOQMF010000000
VERSION     DAOQMF010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102310_length_1215_cov_2.570690"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLV77_10250"
     CDS             complement(<1..433)
                     /locus_tag="PLV77_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16272.1"
                     /translation="MANTAKAKPNNSMMALELARKATKSRVEVLNALKANEVSLEKAL
                     DNPAIGNVRIEMVLRALPCSKTRTTDRSNPISRRNAAAILARAGISPCKPVGSLRNIV
                     TRGNLLNVAHSVSGRHAWIYESDSPPVLVERHTKGTKGRTEI"
     gene            complement(448..813)
                     /locus_tag="PLV77_10255"
     CDS             complement(448..813)
                     /locus_tag="PLV77_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16273.1"
                     /translation="MSTGVNTEAAIDSDLLATDAFNDPSYEAAALIITNRIRTQLGLP
                     EADRFLAGYRNDPRWGNPVVFTIAAGAPLIAAHLEIAEHRIVVDSLDGERSFRLIKGS
                     VVTGFLERFEAGRYPELEA"
     gene            complement(810..>1215)
                     /locus_tag="PLV77_10260"
     CDS             complement(810..>1215)
                     /locus_tag="PLV77_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16274.1"
                     /translation="EKLIPEVATRPLPDLPTQGHVIARQAIGHLDPLTGLVLLDRLVD
                     RSAYGAEKFGQAWRTRDNCQEMCEETTDAVVYGVQETARIHQGLTPLDPDGEQANTAR
                     EHLAIAVRYAALADHFAQLAGAEINGQQPGRK"
BASE COUNT          171 a          346 c          420 g          278 t
ORIGIN      
        1 gtatctcggt tctgcccttc gtccccttgg tgtgacgttc gaccagcacc ggcgggctgt
       61 cgctctcgta gatccatgcg tgtctgccgg acaccgagtg ggcgacgttc agcaggtttc
      121 cgcgggtgac tatgttgcga agcgatccga ccggtttgca cggggagatc ccggcccggg
      181 caaggatggc cgcggcgttt cgtctcgaga tcgggttgct gcggtcggtc gtcctggttt
      241 tgctgcaggg aagcgctctg aggaccattt cgatccggac gtttccgatc gccggattgt
      301 caagcgcttt ctcaagcgac acttcgttgg ctttcagggc attgagaact tcgacccttg
      361 acttggtggc tttgcgggcc agttcgaggg ccatcatgct gttgttcggt tttgctttgg
      421 ctgtgttggc catggggttt cctttcttca ggcttcaagc tcggggtagc ggccagcctc
      481 gaagcgttcg aggaagccgg taacaaccga gcctttgatc agccggaagc tccgttcgcc
      541 gtcgagcgag tcgacgacga tccggtgctc ggctatttcg aggtgcgcgg cgatcaaagg
      601 tgcgccggca gcgatcgtga aaacgaccgg gttgccccag cgggggtcgt tgcggtagcc
      661 ggccaggaac cggtcggctt ccggcaatcc gagctgggtg cggatccggt tggtgatgat
      721 cagtgccgcg gcctcgtagg aggggtcgtt gaacgcgtcg gtggcgagca gatcgctgtc
      781 gatcgccgcc tcggtgttga ccccggtgct cattttcgac ctggctgctg gccgttgatt
      841 tcagcgcctg cgagctgcgc gaaatggtct gcgagggccg cgtacctgac cgcgatcgca
      901 agatgttccc ttgcggtgtt ggcctgttct ccatccggat ccagcggggt gaggccctga
      961 tggatgcggg cggtttcctg gaccccgtag acgacggcgt cggtggtttc ctcgcacatc
     1021 tcctggcagt tgtctcgggt gcgccaggcc tggccgaact tctcggcacc gtaggcgctt
     1081 cggtcaacca gccggtccag caaaaccagc ccggtgagcg ggtcgagatg gccgatcgcc
     1141 tgccgggcaa tcacatggcc ttgggtgggg agatctggaa gcggccgggt ggctacttcc
     1201 gggatgagtt tctct
//
LOCUS       DAOQMF010000686         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_102462_length_1214_cov_3.091458, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000686 DAOQMF010000000
VERSION     DAOQMF010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102462_length_1214_cov_3.091458"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1214
                     /locus_tag="PLV77_10265"
     CDS             <1..>1214
                     /locus_tag="PLV77_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16275.1"
                     /translation="FGGLDSSWWVGLYLAAHNQMQFGTEIVFTYGPLGILRFPWLLYP
                     GALAVIPFVYASILHVGFSSALVAALRRRVGLLWAMLVALLVVVQLEWVEQEVAISIL
                     AAMLMIERRPAGRKLLFFAVCAGLLAGTEMLVKLSTGPVIAAVLLVGLVGARARTREI
                     AAYLGTLAGSMLVCWFVTGQDLSNLPDFIGNSIQIVLGYQEAMAVYGQSGWYSLIIVA
                     GGALCFAWALFGGYADRRARIAGALVAAIVIFVFYKQAVVRIDSAHIALFFALIAVLW
                     LAVPPRQGFAGISLAGLAFMGVVSIYAATGVPSPGLNLISNLRGFYEESRTALSPARQ
                     RAGVDFWRQGIRNNYQLDPKLLAELEGQSVSVEPWETTAAWAFELDWHPAPVFQNYSA
                     YTSKLDELNSDT"
BASE COUNT          165 a          404 c          401 g          244 t
ORIGIN      
        1 ccttcggtgg tctcgactcg agctggtggg tcggtctcta cctcgcggcc cacaaccaga
       61 tgcagttcgg gaccgagatc gtcttcacct acggtccgct cggaatcctc cgtttcccct
      121 ggctgctcta cccgggcgcg ctggcggtga tcccgttcgt ttacgcatcg atcctgcatg
      181 tcggtttcag ctcggccctg gttgcggcac tgaggagaag ggtcggtctg ctctgggcga
      241 tgctggtcgc cctgctcgtg gtggtccagc tcgagtgggt cgagcaggag gtggcgatca
      301 gcatcctggc ggcgatgctg atgatcgagc ggcgcccggc cggccgcaag ctgctcttct
      361 ttgcggtctg tgccggattg ctggccggga ccgaaatgct ggtcaagctt tcgaccggcc
      421 cggtgatcgc cgcggtcctc ctggtcggac tggtcggggc gcgggcgcgg acccgcgaga
      481 tcgcggccta cctcggcacc ctggccgggt cgatgctggt ctgctggttc gtgaccggcc
      541 aggacctctc gaacctgccc gacttcatcg gcaactcgat ccagatcgtg ctcggctatc
      601 aggaggcgat ggcggtctac ggccagtccg gctggtactc gctgatcatc gtcgccggcg
      661 gagcgctctg tttcgcctgg gccctgttcg gcggttacgc cgaccgccgg gcccggatcg
      721 ccggtgccct ggtcgcggcg atcgtgatct tcgtcttcta caagcaggcg gtggtgcgga
      781 tcgacagcgc ccacatcgcc ctgttcttcg ccctgatcgc cgtgctctgg ctggccgtcc
      841 cgccgcgaca ggggttcgcc ggaatctcgc tggccggact ggccttcatg ggtgtggttt
      901 cgatctatgc ggcgaccggc gtgccttccc ccgggctgaa cctgatttcc aacctgaggg
      961 gtttctacga ggaaagcagg acggccctca gcccggcccg tcagcgggcc ggcgtcgact
     1021 tctggcgcca gggaatccgg aacaactacc agctcgaccc gaagctgctg gccgagctcg
     1081 aaggtcagag cgtcagcgtc gaaccctggg agaccacagc cgcctgggcc ttcgaactcg
     1141 actggcatcc ggcaccggtc ttccagaact acagcgccta cacctcgaag ctcgatgagc
     1201 tgaattccga cacg
//
LOCUS       DAOQMF010000687         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_102655_length_1213_cov_2.986183, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000687 DAOQMF010000000
VERSION     DAOQMF010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102655_length_1213_cov_2.986183"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLV77_10270"
     CDS             <1..579
                     /locus_tag="PLV77_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16276.1"
                     /translation="FNHTYKVYEGRNLSGMTALDISRGTVVPGFGIAIGTDGAGAHID
                     ADTYFNAVDRYGSPGWDKEEIEAASGEDRSRIDTILVGALRVRLIPAKRVSPLPRTCR
                     IAEATEAIGDPVTVTSPVLLIEPEKTVGIYLGRFGPDASAPAWGAVGGVPTGYLIPSG
                     RSDRPWRIGFKGEGKVRVCDARLAPETRDPEK"
     gene            610..>1213
                     /locus_tag="PLV77_10275"
     CDS             610..>1213
                     /locus_tag="PLV77_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16277.1"
                     /translation="MPEERPDRIWIWLLGLAMLASGVLLMAMRSQIGFFLDDWDLVIY
                     RGNPTDWLLPHNEHIVVLPAAIYKLSLAIFGMTAIPLHLVAVALFLTSVAALFLWLRP
                     LTGAPSAFFGCLVVLFLGAATEDLVWAFQMGFFGSVAAGLGALLQLRRGTRTGDRWAC
                     LLLVVSVLFSSLGIPFTLGAAVLILFGESLHPDWRRFPGRA"
BASE COUNT          170 a          412 c          401 g          230 t
ORIGIN      
        1 ttcaatcaca cctacaaggt ctatgagggc cgcaacctga gcggaatgac cgcgctggac
       61 atctcccggg gaacggtcgt tcccggcttc ggaatcgcga tcggaaccga tggtgccggt
      121 gcccacatcg acgccgatac ctacttcaac gcggtcgacc gctacggctc gcccggctgg
      181 gataaagagg agatcgaggc ggcttcggga gaggatcgaa gccggatcga cacgatcctg
      241 gtcggcgccc tgcgggtccg gctgattccc gcaaaacggg tatccccgct tccccggacc
      301 tgccggatcg ccgaggcgac ggaggcgatc ggtgatccgg tcaccgtcac ctctccggtg
      361 ctcctgatcg agccggagaa gacggtcggc atctaccttg gccgcttcgg gccggacgca
      421 tcggcaccgg cctggggggc ggtgggtggc gtcccgaccg gttacctgat cccttccggc
      481 cgctccgacc ggccctggcg gatcggcttc aagggagaag gcaaggtgag ggtctgcgac
      541 gcccggctcg cccccgaaac ccgcgatccg gaaaagtaac ctgcccccat gggtgcggcg
      601 tcgagcgaaa tgcccgaaga aagaccggac cggatctgga tctggctgct aggcctggcg
      661 atgctggcct cgggcgtcct gctgatggcg atgcggtcgc agatcggctt cttcctggat
      721 gactgggacc tggtcatcta ccgtggcaac ccgaccgact ggctgttgcc ccacaacgag
      781 cacatcgtgg tgttgccggc cgcaatctac aagctgtccc tggcgatctt cgggatgacc
      841 gcgatcccgc tccacctggt ggccgtcgca ctgtttctga cctcggtggc ggcgctgttc
      901 ctctggcttc gccccctgac cggcgcgccc tctgccttct tcggctgcct ggtcgtcctc
      961 ttcctcggag cggcgaccga agacctggtc tgggctttcc agatgggctt cttcggctcg
     1021 gtcgccgccg gtctcggggc gctgctccag ctgcggcggg gaacccggac gggggaccgg
     1081 tgggcctgcc tgctgctggt ggtctcggtt ctcttctcct cgctcggcat ccccttcacg
     1141 ctcggcgcgg ccgtcctgat tctgtttggc gagagccttc acccggactg gaggcggttc
     1201 cccggccgtg cct
//
LOCUS       DAOQMF010000688         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_102836_length_1212_cov_2.592913, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000688 DAOQMF010000000
VERSION     DAOQMF010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102836_length_1212_cov_2.592913"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..>1212
                     /gene="murF"
                     /locus_tag="PLV77_10280"
     CDS             164..>1212
                     /gene="murF"
                     /locus_tag="PLV77_10280"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNC16278.1"
                     /translation="MIELTTEQMAAAMGADVIARGSGGVPERAEIDSRKVEGGELFFG
                     LTGGQSDGGEHAANALESGAWGVVVSHERAAELVAGGPEPGNSGAPGAFVFASPDPLL
                     SLQMLARAWRRELDATVIGITGSVGKTSVKDITRALLPGEVHASAENFNTEIGLPLTI
                     LEAPAGTETLVLEMAMRGEGQIAELAVIAEPDVGVITNVGPVHVELLGSIEAVAAAKA
                     ELIVNMRPEGVMIVPFEAGYLDPHLENVPGLIRFGGEGDVRVIELSVENGATKARIET
                     LQGSQAFEFPFAEPHNLTNSLAAIGAGVAAGFTPAELAARADGIAFSRFRNEHIDLGD
                     RGLIINDCYNANPVS"
BASE COUNT          227 a          366 c          419 g          200 t
ORIGIN      
        1 ccgcgatcgg cctggcgatc agggaagccg gaccggggga cacggtggtg atcgccggca
       61 atggccacga gcagggccag gaattcgaga acggacggaa gatccccttc gacgatcgcg
      121 acgtagcccg cagacacctt cgcgaactgg ctggaaagcc cgcgtgatcg agctgaccac
      181 cgaacagatg gcggccgcga tgggagcgga tgtgattgcc cgcgggtcgg gcggggtgcc
      241 ggagcgggcc gagatcgatt ctcgcaaggt tgaaggaggc gagctcttct tcggcctgac
      301 cggaggtcag agcgacggcg gcgagcacgc ggcgaacgcg cttgagtccg gggcctgggg
      361 ggtcgtcgta agccacgaac gggcggcaga gctggtcgcc ggcgggccgg agccaggcaa
      421 ctccggtgcg cccggcgcat ttgttttcgc cagccccgac cccctgcttt cacttcagat
      481 gctggcccgc gcctggcgca gggaactcga tgcgacggtc atcggaatca ctggatcggt
      541 aggcaagacc tcggtcaagg acatcacccg ggcgttgctg cccggtgagg tccacgcctc
      601 ggccgagaac ttcaatacgg agatcggcct gcccttgacc attctcgaag cgccggccgg
      661 aaccgagacc ctggtcctgg aaatggcgat gcggggcgag ggacagattg ccgaactggc
      721 ggtgatcgcc gaaccggatg tgggagtgat caccaatgtg gggcctgttc acgtcgaact
      781 gctcggttcg atcgaggcgg tggccgcggc caaggcagag ctgattgtca acatgagacc
      841 cgaaggagtg atgatcgttc ccttcgaggc cggttacctc gacccgcacc tcgaaaacgt
      901 gccgggcctg attcgcttcg gcggggaggg tgatgtgcgg gtgatcgaac tctcggttga
      961 aaacggggcg accaaggccc ggatcgagac ccttcagggt tcgcaggcct tcgaatttcc
     1021 cttcgccgaa ccgcataacc tgaccaattc cctggccgca atcggtgccg gtgtggcggc
     1081 cggattcacc ccggcggaac tggcagcccg ggcggatggc attgcctttt cacgcttccg
     1141 caacgaacac atcgacctcg gtgaccgggg tctcatcatc aacgactgtt acaacgcaaa
     1201 tccggtgtcg at
//
LOCUS       DAOQMF010000689         1209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_103268_length_1209_cov_4.296360, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000689 DAOQMF010000000
VERSION     DAOQMF010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103268_length_1209_cov_4.296360"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLV77_10285"
     CDS             complement(<1..612)
                     /locus_tag="PLV77_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNC16279.1"
                     /translation="MAERDYLSAISDHIVVFDGGMGATLEMFDLTQEDYGGLAGKCHE
                     ALVLNRPDVIEGVHTSMIEAGAEVVETDTFQASRIKLDEWGLADHTEEINVKAAEIAR
                     KAAGPDRFVAGSIGPTGHLPASDDPTLGQIRFPELVTVFTEQAGGLVKGGADLIIIET
                     AQDILEVKAAIFGARAAFEEIGRSVPIQVSVSLLPNGGKMLLGT"
     gene            682..1200
                     /locus_tag="PLV77_10290"
     CDS             682..1200
                     /locus_tag="PLV77_10290"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNC16280.1"
                     /translation="MVGAISYVALLRAVNVGGTGKLPMADLRKLAGQLGFGDVRTYIA
                     SGNLLITSGLTEAQVKKQLEAALRDYAGREIGVIVRTAAEMRAVELANPFKSAAYNRI
                     GVLFLDHRLPRENIRKAKGRKNEEIEIGKREIYVHYPDGMGRSKLNLPAAKYGTTRNL
                     NTVSKLADMAGG"
BASE COUNT          227 a          365 c          413 g          204 t
ORIGIN      
        1 ggtgccgagc agcatcttgc cgccgttggg cagaagcgag acggaaacct ggatcgggac
       61 gctgcggccg atctcctcga aggcggcgcg ggctccgaag atcgccgcct tgacctcgag
      121 gatgtcctgg gcggtttcga tgatgatcag gtcggcgccg cccttgacca ggccgccggc
      181 ctgttcggtg aagacggtga ccagctcggg gaagcggatc tgcccgaggg tcgggtcatc
      241 cgaggcgggc aggtgcccgg tcggtccgat cgagccggca acgaaacggt ccgggccggc
      301 tgccttgcgg gcgatctcgg ccgccttgac gttgatttcc tcggtgtggt cggcgagacc
      361 ccactcgtct agcttgatcc gggaagcctg gaaggtgtcg gtctcgacca cctcggcgcc
      421 ggcttcgatc atcgaggtgt gaaccccttc gatcacgtcg ggccggttca ggaccagggc
      481 ctcgtgacat ttgccggcca ggccgccgta atcctcctgg gtcaggtcga acatctccag
      541 ggtcgcgccc atcccgccgt cgaagacgac gatgtggtcg gaaattgcgc tcagatagtc
      601 acgttcggcc atcaagcgag gataccgatc agctaaatcc gatcaggatt cgggatttcg
      661 cgttgcccta gtcttaggac catggtgggg gccatttcat acgtcgcgct gctcagggcc
      721 gtgaacgtcg gcggcaccgg caagctgccg atggccgatc tgcggaaact ggccggacag
      781 ctcggtttcg gggatgtgag gacctacatc gccagcggca atctgctcat cacttccggg
      841 ctgaccgaag cgcaggtgaa aaagcagctc gaggcggcgt tgcgtgacta cgcggggagg
      901 gaaatcggcg tgatcgtccg gaccgcggcg gagatgcggg cggtcgagct ggccaatccg
      961 ttcaagagcg ccgcctacaa ccggatcggc gtcctgtttc tcgatcaccg cctgccgcgg
     1021 gagaacatca ggaaggcgaa gggccggaag aacgaggaga tcgaaatcgg caaacgggag
     1081 atctacgtcc actacccgga cgggatgggc aggtcgaaac tgaacctccc cgccgccaag
     1141 tacggcacaa cgagaaacct gaacacggtc agcaagctgg ccgacatggc cggcggctga
     1201 tcagtcccg
//
LOCUS       DAOQMF010000690         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_104109_length_1204_cov_3.830287, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000690 DAOQMF010000000
VERSION     DAOQMF010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104109_length_1204_cov_3.830287"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PLV77_10295"
     CDS             complement(<1..978)
                     /locus_tag="PLV77_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16281.1"
                     /translation="MADMVFHGFGKHSDAIDDQPEPGLTIRKSCFGYNLVVISCLDTC
                     LPVIWTLMPATGNEVAAARELIEALFKLRPEFPMEYLVGDGLYNRGFEFPSTLYKNYG
                     IHLVSPRTNRKSKSEWADSDGVPRCAHGLMKFTRHGEVWDAAKRRAHGIRPGNLPPNG
                     KGLNPRLRFECHEEVCREKSTRMEDDWILHTFLPHIGQDKWTALREVLLARRNTSESL
                     FAQLKQLGVGAKWPNRARWATDNGMRWLTSLALLNMTARKLVHLDGSYAAALKEAYSA
                     GIITRQQMHRFLEVEGIETDSRNRVDDTSPVEPSTWNDWVELPIDREFLA"
     gene            958..>1204
                     /locus_tag="PLV77_10300"
     CDS             958..>1204
                     /locus_tag="PLV77_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16282.1"
                     /translation="MEDHVGHSPMLTRDLADQSLPGARGPHPWECLGNVRLDQCSVDG
                     TEPTEVGEPEFAGYLRRGDLVEEIQVDVNGRPRLSTVS"
BASE COUNT          201 a          345 c          398 g          260 t
ORIGIN      
        1 cgcaaggaac tctcgatcga tcgggagttc gacccagtcg ttccaggtag acggttcgac
       61 cggggaggtg tcgtccactc ggtttcgaga gtccgtttcg atgccctcaa cctcgaggaa
      121 tcgatgcatc tgctgccggg tgatgatgcc ggcgctgtag gcctccttga gggcagcggc
      181 gtagctgccg tcgaggtgga cgagcttgcg ggcagtcatg ttcaagaggg ccagcgaagt
      241 gagccagcgc atgccgttgt cggtggccca cctggctcgg ttcggccact ttgcgccgac
      301 gccgagttgc ttgagctggg cgaagaggga ctcggatgtg ttgcgcctcg ccagcaggac
      361 ttccctgagc gcggtccact tgtcttgccc gatatgaggc aggaaggtgt ggaggatcca
      421 gtcgtcctcc atacgggtgc tcttctctcg gcagacttcc tcgtggcact cgaaccgaag
      481 ccgcgggttc aggcccttcc cgtttggagg aaggtttccc gggcgaatgc catgagctct
      541 ccgcttcgcg gcatcccaga cctcgccatg tcgggtgaac ttcatgaggc cgtgtgcgca
      601 ccggggcaca ccgtcgctgt cggcccattc gctcttcgac ttcctgttgg ttcttggaga
      661 gaccaggtgg atgccgtagt tcttgtagag ggtgctcgga aattcgaagc ccctgttgta
      721 gagaccatcc ccgacgaggt actccatcgg gaattcaggc cgcagcttga aaagggcctc
      781 gatgagttcc ctcgcggcgg cgacctcgtt tcctgtcgcg ggcatgagcg tccagatcac
      841 cggcaggcag gtgtcgagac agctgatgac gacgaggttg tagccgaagc aactcttccg
      901 gatagtgaga ccgggttcgg gctgatcgtc gatggcgtcg gagtgctttc cgaagccgtg
      961 gaagaccatg tcggccattc gccgatgctt acccgcgatc tcgcggatca gagtcttcct
     1021 ggcgcgaggg gacctcatcc ttgggagtgt ctggggaacg ttcgcctcga tcagtgttcc
     1081 gtcgatggca cagaaccgac cgaagttggg gaaccggagt tcgccggcta ccttcgtcgc
     1141 ggcgatctgg tcgaggagat ccaggttgat gtgaatggtc gcccccggct ttccacagtc
     1201 agtg
//
LOCUS       DAOQMF010000691         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_104504_length_1202_cov_2.773322, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000691 DAOQMF010000000
VERSION     DAOQMF010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104504_length_1202_cov_2.773322"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLV77_10305"
     CDS             complement(<1..708)
                     /locus_tag="PLV77_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNC16283.1"
                     /translation="MRSLIEDRFSSLRFASLRIIQICLAAGVAWLIATRVVGHYEPFF
                     APISVVLVLGLAIEQRGRRAYEIAFGVALGIAIADLIVLALGTGTWQLMLVIGLAMSA
                     AVLSGGGVMLVNQAAISGVLVATLPAPDLADTTHRFVDALVGGGVALAANAITPVDPV
                     RLVRRYLEPLLARLSGTLVDIADALDHESHEEIVAALERARSLDPAVREMKIAVEAGQ
                     ETISLAPTRRRKRGRVDQ"
     gene            complement(818..>1202)
                     /locus_tag="PLV77_10310"
     CDS             complement(818..>1202)
                     /locus_tag="PLV77_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16284.1"
                     /translation="HDMTEPLGLEEVFSHREDEDLNGPLPPGTRIGHIHLYVGDLDAA
                     RTFYEEIGFSPHMNADGIGFADLSLGGTFPHRIAVNVWQGIGAPQAPEGTAGMRCFEL
                     DDPRGGEVRELADPAGNRLIIRPAA"
BASE COUNT          194 a          404 c          404 g          200 t
ORIGIN      
        1 ctgatcgacg cgcccccgtt ttcgtcggcg ggtcggggcg agtgaaatcg tttcctgacc
       61 ggcctcgacg gcgatcttca tttctcgcac cgccgggtcc agggaccggg cccgctccag
      121 ggcggcgacg atttcctcgt gactttcgtg gtcgagcgca tcggcgatgt cgaccagagt
      181 ccccgaaagc cttgcgagca gcggttccag gtagcggcgc accaggcgaa ccggatcgac
      241 cggggtgatt gcgtttgcgg cgagcgcaac gccgccgccg accagggcgt caacgaaccg
      301 gtgggtcgtg tcggccaggt ccggggccgg cagggtcgca accagcaccc cggaaatggc
      361 tgcctggttg acgagcatca ccccgccgcc ggaaagcact gccgctgaca tcgccagccc
      421 gatgaccagc atcagctgcc aggtgccggt cccgagcgcc agcacgatca ggtcggcgat
      481 cgcgatgcca agggccaccc cgaatgcgat ttcgtaggcc cgccggcctc gctgttcgat
      541 cgccaggccc agcaccagga ccaccgagat cggcgcgaag aacggttcgt agtgcccgac
      601 gacccgggtt gcgatcagcc atgcgacgcc cgctgccagg cagatctgga tgatcctcag
      661 cgaggcgaag cgcagggagg agaaccggtc ttcgatcagc gatcgcacgg ccccactatt
      721 tcaccgggtc gtccggaacc acgcggttta gagcttctgc gtctgctttt tcgtcttctg
      781 cacgctccag ccgtggccgg ccggcggctg gcgacggtca ggctgcaggt ctgatgatca
      841 ggcggtttcc ggccgggtcg gccagttcgc gcacttcccc gccccgcgga tcatcgagtt
      901 cgaagcaacg catgccggcc gttccttccg gggcctgcgg ggcaccgatc ccctgccaga
      961 cgttcacggc aatccggtgg ggaaatgtgc cgccgagcga aaggtcggcg aaaccgatcc
     1021 cgtcagcgtt catgtgcggg ctgaaaccga tctcctcgta gaaggtccgg gctgcgtcga
     1081 ggtcccccac gtagagatgg atatgcccga tccgggtgcc cggcggcagc ggcccgttca
     1141 ggtcttcgtc ctcgcggtgg gagaagacct cctcgaggcc gagcggctcg gtcatgtcgt
     1201 gg
//
LOCUS       DAOQMF010000692         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_104626_length_1201_cov_3.599476, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000692 DAOQMF010000000
VERSION     DAOQMF010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104626_length_1201_cov_3.599476"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PLV77_10315"
     CDS             complement(<1..1167)
                     /locus_tag="PLV77_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16285.1"
                     /translation="MPKLSLDGVIWSDLDADFLDFDLKLPGFGPAPVKLVWATRPAFH
                     VTQLLSLRIAIGPGQGTLRSANDAELATLTGISLSYDGGTGQFTVAGKVGTMANPIER
                     DLDEVVGSDPGGRLPVTIDISEAKMAVTTGAGFDLSDPGSTANASAQAKLTLGRVVVR
                     SKEDPRLFIAFAAEFDASAAAAVGGNPTVGARLSSLKVIEPYPVELICAAGTTAIEGV
                     LRLVAMIKLPKTTVPDVPGLPFPDLSNLARVVDRLSDLLVSALKWMARQAGEAGTALS
                     QLAEAGIEALGRLIRMLRDAADSVLSHVVIEVRLDAKTCHLRQILISPVSMGQGGSSE
                     HYSTNAYGLEFTVPHSWRPSLLIDVSGPFFAALLVEPSSDELTIANDLWLERDGA"
BASE COUNT          254 a          389 c          365 g          193 t
ORIGIN      
        1 ggccccgtcc cgttcgagcc aaaggtcgtt cgcaatcgtc aactcatcgc tcgacggttc
       61 gacgagaagc gccgcgaaga acggtcccga gacgtcgatc aacaggctcg gccgccaact
      121 gtgcggaacg gtgaactcga gcccgtaagc gttcgtcgaa tagtgttcag acgatccacc
      181 ctgtcccatc gaaaccggcg agatcaggat ctgacgaaga tggcaggtct tggcgtcaag
      241 cctcacctcg atcaccacat gtgacagcac gctgtccgcc gcgtcacgga gcatccggat
      301 caatcgcccg agcgcctcaa tcccggcctc cgcgagttgc gagagcgcag tgcccgcttc
      361 accggcttgg cgcgccatcc acttcagcgc agagacaagc agatcgctca gacgatcgac
      421 aacacgcgcc agattagaga ggtcgggaaa tggcaagccc ggcacgtccg gaaccgtcgt
      481 ctttggcagc ttgatcatcg ccaccaggcg cagcacaccc tcgatcgcag tcgtcccggc
      541 cgcacagatc agttcaaccg gatagggctc gattaccttc agcgaggaga gccgcgcacc
      601 gacggtcggg ttgccgccga cggccgcagc agccgaggca tcgaactcgg cggcgaaggc
      661 gatgaatagg cgcgggtctt ccttggagcg gacgacaacc cgcccgagcg tcagcttggc
      721 ctgggcactg gcattcgcgg tcgaacccgg atcggaaaga tcaaaaccag cgccggtggt
      781 gaccgccatc ttcgcctcgg aaatgtcgat cgtgaccggc aagcgtccgc cgggatcgga
      841 gcccacaacc tcatcgagat ccctttcgat cgggttggcc atcgtgccga ccttcccggc
      901 caccgtgaac tggcccgtcc cgccatcgta gctaagcgag atacccgtca gggtcgccaa
      961 ttcagcatcg ttggcagacc ggagtgttcc ttggcccggt ccgatcgcga tcctgagcga
     1021 taaaagttgg gttacgtgga aggccggccg tgtggcccaa acgagcttga ctggggccgg
     1081 tccgaaaccg ggcagcttca gatcgaagtc aaggaagtcc gcatcaaggt ctgaccagat
     1141 cacaccatcg agcgaaagct tgggcaactg aaccgtcggg aatcgcaagc gcgggcgcgg
     1201 t
//
LOCUS       DAOQMF010000693         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_105057_length_1199_cov_1.949301, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000693 DAOQMF010000000
VERSION     DAOQMF010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105057_length_1199_cov_1.949301"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLV77_10320"
     CDS             <1..773
                     /locus_tag="PLV77_10320"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNC16286.1"
                     /translation="RTREFEQMEMEFFVPPAEAQQWWEKWVEIRHNWYLELGIRPDFL
                     MIRPHEADELSHYSSGTSDVEYLFPIGWSELEGVANRGNFDLTQHQEHSGESMEFFDQ
                     ATGERYIPHVIEPAAGADRAALAFLVDSYKEEEIEGRKRTVLQLHPRLAPVKVAVMPL
                     FNKEGMPETAREINAALRKAGIQTEYDTGGSIGKRYRRQDEIGTPWGITVDFDTKDDG
                     QVTLRDRDSLEQVRVPIEGLPELIAGKLAEPWQSPKLG"
     gene            complement(774..>1199)
                     /locus_tag="PLV77_10325"
     CDS             complement(774..>1199)
                     /locus_tag="PLV77_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16287.1"
                     /translation="GKLIDEILDSLYRDVSGGSTRPVDGLGLHEPGIGYWPHGKGDTW
                     HRRFVFEATRPVDRQDLFSRVAGQLLKDGLSGVLIRGLYLQPPGYGDDPDGPVGHIFT
                     ASGEQETTDERDRSGTEGTPESSIETMIREINSSGILGG"
BASE COUNT          229 a          399 c          366 g          205 t
ORIGIN      
        1 tccggacccg cgagttcgag cagatggaga tggagttctt cgtgccgccg gccgaggctc
       61 agcagtggtg ggagaagtgg gtcgagattc gccacaactg gtacctcgag ctcggcatcc
      121 ggcccgactt cctgatgatc cgcccgcatg aggccgacga gctttcgcat tactcgtccg
      181 gcacctcgga tgtcgagtac ctgttcccga tcggctggtc ggagctcgaa ggagtcgcca
      241 accgcggcaa cttcgacctg acccagcatc aggagcattc gggcgagagc atggagttct
      301 tcgaccaggc cacaggtgag cggtacatcc cgcatgtgat cgaaccggcc gccggcgccg
      361 accgggccgc gctcgccttc ctggtcgatt cctacaagga ggaggagatc gagggccgca
      421 agcggacggt tctccagctc cacccccggc tggccccggt caaggtcgcg gtgatgccgc
      481 tcttcaacaa ggagggcatg ccggagaccg cccgggagat caacgccgcc ctgcggaaag
      541 ccgggatcca gaccgaatac gacaccggcg gctcgatcgg caagcgctac cgccgccagg
      601 acgagatcgg caccccctgg ggaatcaccg tcgacttcga caccaaggac gacggccagg
      661 tcaccctgcg tgaccgcgac agcctcgaac aggtccgggt cccgatcgaa ggcctgcccg
      721 aactgattgc cggcaagctc gccgagccct ggcagtcccc caagctgggc tgatcagcca
      781 cccaggattc cgctcgagtt gatttcgcgg atcatcgttt cgatggacga ttcgggcgtt
      841 ccctcagttc ccgatcggtc acgttcgtct gtggtctctt gctcaccgga agcagtgaag
      901 atgtgcccga cgggaccgtc cgggtcatct ccatagccgg gcggctgcaa atagagcccc
      961 ctgatcagga cgccggagag accgtccttc agcaactggc ccgccacccg gctaaagagg
     1021 tcttgcctgt caactggtcg agttgcttcg aatacaaatc gacggtgcca cgtgtcgccc
     1081 ttgccgtgcg gccaataacc aattcccggc tcgtgaaggc cgagaccgtc gacggggcgc
     1141 gtacttccac cgctgacgtc tcgatacagc gaatcgagaa tctcgtcaat caacttccc
//
LOCUS       DAOQMF010000694         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_105106_length_1198_cov_4.174978, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000694 DAOQMF010000000
VERSION     DAOQMF010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105106_length_1198_cov_4.174978"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLV77_10330"
     CDS             complement(<1..561)
                     /locus_tag="PLV77_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNC16288.1"
                     /translation="MRNPRNFFKPKAIGAPDPITQVPFGPSRMIHFFDASNEKMAAKV
                     PDMATQADILLGNLEDAVPVDNKEAARAGLVKVAREADLGGTPLWTRINSLESPWVLD
                     DLTTLVTEVGNKIDVIMVPKVEGPWDIHYVDQLLAQLEAKGEVTRPILIHAILETAQG
                     VTNVEDIAAASPRMQGMSFGPADLAAS"
     gene            755..>1198
                     /locus_tag="PLV77_10335"
     CDS             755..>1198
                     /locus_tag="PLV77_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC16289.1"
                     /translation="MAGLLQPVTITNRGSARLTISGPPVITGSNPGDFGVVSNRCSGR
                     ALGFGDSCRVWVAATPQGEGLRVGRLTLPSNSSTVIQADLIVVGTVSPIGATGPTGPT
                     GSSGSTGTTGPTGGTGPTGPTGGTGPTGPTGPTGVTGPRGPAPSVR"
BASE COUNT          183 a          370 c          439 g          206 t
ORIGIN      
        1 cgaggcggcg aggtcggccg gtccgaagct catgccctgc atgcgggggc tggccgcggc
       61 gatgtcctcg acgttggtca cgccctgggc ggtctcgagg atggcgtgga tcaggatcgg
      121 ccgggtcact tcgcccttgg cctcgagctg ggcgagcagc tggtcgacgt aatggatgtc
      181 ccatggacct tcgaccttcg ggaccatgat cacgtcgatc ttgttgccga cttcggtgac
      241 cagggtggtc aggtcatcga gcacccaggg gctctcgagc gagttgatcc gggtccagag
      301 gggcgtgccg ccgagatcgg cttccctggc gaccttgacc aggccggcgc gggcggcttc
      361 cttgttgtcg accgggaccg catcctcgag gttgccgagc aggatgtccg cctgggttgc
      421 catgtccggc accttggccg ccatcttctc gttcgaggcg tcgaagaagt ggatcatccg
      481 ggaggggccg aaggggacct gggtgatcgg gtcgggggcg ccgatcgcct tgggcttgaa
      541 gaagttccgg gggttacgca aggttctcct tgagggttgg ttagagaaga gctattcggc
      601 gcgaaaagca gcatcagcgc caccgaaaag cgtataggga tcgcggctcg cgggccctct
      661 gggtcgcatg tcgctgttga ctggtccgcg ggcgacatat atcttggcga ggtgccacga
      721 aaggacgcgg ggatcagggg ctttatcagg ggcgatggca ggtctgctcc agccggtcac
      781 gatcacgaac cgtggttcgg cccggctgac gatctccgga ccaccggtga ttacaggttc
      841 caaccccggt gacttcggag tcgtgtccaa ccgctgttcc ggccgggccc tgggcttcgg
      901 tgactcctgc cgggtctggg tggcagcgac gccccagggc gagggactgc gggtgggcag
      961 gctgaccctg ccctcgaact cttcgaccgt gatccaggcg gatctgatcg tggtcggaac
     1021 cgtaagcccg atcggcgcaa cgggccccac gggaccaacg ggaagcagcg gcagcaccgg
     1081 caccaccggg ccgaccggcg gtaccgggcc gaccggaccc accggcggta ccgggccgac
     1141 cggacccacc ggcccgacgg gagtgaccgg tccgcggggt cccgccccgt cggtcagg
//
LOCUS       DAOQMF010000695         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_105493_length_1196_cov_2.707274, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000695 DAOQMF010000000
VERSION     DAOQMF010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105493_length_1196_cov_2.707274"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1168
                     /locus_tag="PLV77_10340"
     CDS             26..1168
                     /locus_tag="PLV77_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HNC16290.1"
                     /translation="MTLSMPADSTTLDEEGVIIPPRILDEESILEVVTLMRQPEQRRA
                     DLRAQLAANRAGVGRLEAMASDRGIDGLARAMDDVISYAENRTRAEIERIPDGTYCAT
                     DLLEAREGDLELRLEATVSGSSIRFDFTGSSGPDSGNLNCPRAVTESACFFAIRVLTD
                     PDIPASSGAWRPVEVVVPDGSLLSAQPPSAVVAGNVETSSRVADLCLSAFGLAQGQGT
                     MNNLTPGGENFTYYETLGGGQAASDRADGPSAAHVAMSNTLNTPIEALENEFPLRAGE
                     YSVRRGSGGEGGHRGGDGLTREIVAEEQISFALLTERRRHAPAGAEGGAPGERGRNLL
                     VRKDGSAEDLEPKSAGRLEPGDRLRIETPGGGGFGTGGGAGSSLDA"
BASE COUNT          206 a          406 c          413 g          171 t
ORIGIN      
        1 ccactgcttg gccagggcca ggtaggtgac tttgtcgatg ccggccgact cgaccaccct
       61 cgatgaagag ggagtgatca tcccgccccg gatcctcgac gaggagtcga tcctcgaagt
      121 ggtcaccctg atgcgccagc cggagcagcg ccgggccgac ctgcgggccc agctagcggc
      181 caatcgggcc ggggtcggtc gcctggaagc gatggcgtcc gaccggggga tcgacggact
      241 cgcaagagcg atggacgatg tgatcagcta cgcggagaac cgcacccggg ccgagatcga
      301 acgaatcccc gacgggacct actgtgccac cgatctgctc gaagcccgcg aaggcgacct
      361 cgaactgcgg ctcgaagcga cagtttcagg aagcagcatc cgcttcgact tcaccggttc
      421 ttccggtccc gacagcggca atctcaactg cccgagagcg gtcaccgagt cagcctgctt
      481 cttcgcgatc cgggtcctga ccgaccccga catcccggcg tcctccggcg cctggagacc
      541 ggtcgaggta gtggttcccg acggctcgct gctcagcgcc cagccgccca gcgcggtggt
      601 cgccggcaac gtcgagacct cgtcccgggt ggccgacctc tgcctcagtg ccttcgggct
      661 cgcccagggc cagggcacga tgaacaacct cacccccggc ggcgagaact tcacttacta
      721 cgagactcta ggcggaggcc aggccgcctc ggatcgggcc gatggcccct ccgccgccca
      781 tgtggcgatg tcgaacactc tgaacactcc gatcgaagcg ttggaaaacg agttcccgct
      841 gcgagccggc gagtactcgg tcaggcgggg cagcggcggc gagggcgggc accggggcgg
      901 cgacggcctg actcgcgaga tcgttgccga agagcagatc agcttcgccc tgctgaccga
      961 gcgccggcga catgccccgg ccggagccga aggtggtgct cccggtgagc ggggccggaa
     1021 cctgctggtc cggaaagatg gctcggccga ggatctcgaa ccgaagtcag ccggtcggct
     1081 cgaaccggga gaccgcctga gaatcgagac tccgggcggg ggcggtttcg gaaccggtgg
     1141 cggcgcgggg tccagccttg atgcctgaga gcccggcaca ggcgatcctc ggggtc
//
LOCUS       DAOQMF010000696         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_105692_length_1195_cov_3.003509, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000696 DAOQMF010000000
VERSION     DAOQMF010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105692_length_1195_cov_3.003509"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLV77_10345"
     CDS             complement(<1..598)
                     /locus_tag="PLV77_10345"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNC16291.1"
                     /translation="MKHLLSIDDLDQEQIEALLDRAESFAEVSRRDIKKVPALRGRTI
                     VTLFYEASTRTSSSFELAAKRLSADVVSVKASGSSVEKGESLKDTVETLSAYDPAAIV
                     IRHPQAGAAQMLSHWTDAAIINAGDGMHQHPSQALLDLFALRQRIGSVEGRKVWIVGD
                     VLHSRVARSNIWAFSMMGADVTIAGPPTLIPRGIEASGC"
     gene            complement(595..1149)
                     /gene="pyrR"
                     /locus_tag="PLV77_10350"
     CDS             complement(595..1149)
                     /gene="pyrR"
                     /locus_tag="PLV77_10350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNC16292.1"
                     /translation="MNEKVVLEANDVERTLRRIAHEIVEKNPDVSDLAIVGIHTGGAY
                     LAERLNSMIAGLIGEAPPLGEIDISFYRDDIGIRGSDYHPVVHSTRLDFPLQDRTVVL
                     VDDVLFTGRTVRAAIEALFDFGRPARIQLAVLCDRGHRELPIRPDYVGKNLPTSRSEQ
                     VSVRLKETDGEDGVAILSPVEAVE"
BASE COUNT          212 a          361 c          418 g          204 t
ORIGIN      
        1 cgcaaccgga agcttcgatt ccccgcggga tcagggtcgg cggaccggcg atcgtcacgt
       61 cggcgcccat catcgagaag gcccagatgt tcgagcgggc gacccggctg tgaagcacgt
      121 cgccgacgat ccagaccttc ctgccctcga ccgagccgat ccgctggcgg agggcgaaaa
      181 ggtcgagcag cgcctggctg ggatgctgat gcatgccgtc gccggcgttg atgatcgcgg
      241 cgtcggtcca gtggctcagc atctgcgcgg cgccggcctg ggggtgccgg atcacgatcg
      301 cggccgggtc ataggcggaa agggtttcga ccgtgtcctt cagcgactcg cccttctcga
      361 ccgaggagcc cgatgccttg accgaaacga cgtcggcgga gagtcgtttg gccgccagct
      421 cgaaggagga tgaggtccgg gtcgaagcct cgtaaaagag ggtgacgatg gtgcggccgc
      481 gcagggccgg caccttcttg atgtcccgcc tggagacttc ggcaaagctt tcggcccggt
      541 cgagcagggc ttcgatctgt tcctggtcca ggtcatcgat gctgagcaga tgtttcattc
      601 gaccgcctcc accggcgaga ggatggcgac cccgtcctcg ccatcggttt ccttgagtcg
      661 caccgagacc tgctcggagc gcgaggtcgg caggttcttg ccgacatagt ccggccggat
      721 cggcaactcg cggtgaccgc gatcgcagag cacggcaagc tggatgcggg cgggccggcc
      781 gaagtcgaag agcgcctcga tcgcggcccg gaccgtgcgg ccggtaaaga gcacgtcatc
      841 gaccaaaacg acggtgcggt cctgaagggg gaaatcgagc cgggtcgagt gaacgaccgg
      901 atggtagtcg gagccgcgaa tgccgatgtc atcccgatag aaggagatgt cgatctcgcc
      961 cagcggcgga gcttcaccga tcaggccggc gatcatcgag ttgaggcgtt cggcgaggta
     1021 ggcgccgccg gtgtggatgc cgacgatcgc gagatccgag acgtcgggat tcttctcgac
     1081 gatctcgtgg gcgatgcgac ggagcgtgcg ctcgacgtcg tttgcttcca gcacaacctt
     1141 ctcgttcaaa gtcaccttcc cggcctcaca ggacctgggt taaaggaacg gggat
//
LOCUS       DAOQMF010000697         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_107587_length_1184_cov_3.727192, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000697 DAOQMF010000000
VERSION     DAOQMF010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107587_length_1184_cov_3.727192"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLV77_10355"
     CDS             complement(<1..498)
                     /locus_tag="PLV77_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNC16293.1"
                     /translation="MSNNNSPEDFPNFLNNPVETVAERLLGCFFERNLGGRRMLARIV
                     ETEAYDEGDAASHSHRGRTARTEVMFGEAGYLYVYFTYGMHYCCNVVTGEAGHGSAVL
                     IRAVEPVEGIDSMEERRGLHGRNLTNGPAKLCQALDIDLRLGGHDLREEPLRLLTGEL
                     LPGESS"
     gene            585..>1184
                     /locus_tag="PLV77_10360"
     CDS             585..>1184
                     /locus_tag="PLV77_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16294.1"
                     /translation="MTLIIGVGRSEAHIYWGDTAGDQISRAENDGTGVDSDFLWWSVG
                     NPTAVAIDGTGIYWANNTDNSVRRANIDGSGETLSAWIPTGITSAWGVAIDSTYAYYT
                     NIAGTSIGRKNRNTGVLDQAFITNTGLQPDSVTVSGSYIYWSHSGNAIGRANIDGTGA
                     DPDFISGLSGVRGIAVNGSYIYWAGDTTNGIGRANLNGTG"
BASE COUNT          226 a          392 c          366 g          200 t
ORIGIN      
        1 cgaggattca cccggaagca gctctccggt caggagccgc aggggctcct cgcgcaggtc
       61 gtgaccgccg agccgcaggt cgatgtcgag ggcctggcag agcttggccg gtccgttggt
      121 gaggttccgg ccgtgaagcc cgcgacgctc ctccatcgag tcgatgccct cgaccggttc
      181 cacggcccga atcagcacgg ccgacccgtg tccggcctcg ccggtgacca cgttgcagca
      241 atagtgcatt ccgtaggtga agtacacgta caggtaaccc gcctcgccga acatgacctc
      301 ggttcgtgcc gtcctgcccc ggtgcgagtg gctggcggca tccccttcgt cgtaggcctc
      361 ggtctcgacg atccgggcca gcatccggcg cccgcccaga tttcgctcga aaaagcaacc
      421 gagcaaccgc tccgcgacgg tttcgaccgg gttattcagg aagttcggga aatcttctgg
      481 actattgttg ttcgacattc ctactcgaga ggcaagacgc tttgacccac agaatcaggc
      541 acatgggggc gatggctgcg tgagcgcttg ccctgctctt cgcattgacc ctaattatcg
      601 gcgtcggccg ctccgaggct cacatctact ggggcgatac ggcgggtgac cagatctccc
      661 gggccgagaa cgacggaacg ggcgttgact ccgacttcct ctggtggtcg gtcggcaacc
      721 cgacggccgt tgcgatcgac ggcaccggga tctactgggc gaacaacacc gacaactcgg
      781 tccgtcgggc gaacatcgac ggcagcggag aaactctcag cgcctggatt ccgaccggca
      841 taaccagcgc ctggggcgtc gcgatcgatt cgacctacgc gtactacacg aacattgccg
      901 gcacttcgat cgggcgaaag aaccggaaca ccggcgtcct cgaccaggcc ttcatcacca
      961 acaccggatt gcagccggac tcggtaaccg tcagcgggtc ctacatctac tggagtcaca
     1021 gtggcaacgc gatcggtcgc gcaaacatcg acggcaccgg ggccgacccc gatttcatca
     1081 gcggcctgag cggagtccgg gggatcgccg tcaacgggag ctacatctac tgggccgggg
     1141 acaccaccaa cggaatcggc cgtgccaacc tgaacgggac cggc
//
LOCUS       DAOQMF010000698         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_107795_length_1183_cov_3.058511, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000698 DAOQMF010000000
VERSION     DAOQMF010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107795_length_1183_cov_3.058511"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="hisD"
                     /locus_tag="PLV77_10365"
     CDS             <1..1000
                     /gene="hisD"
                     /locus_tag="PLV77_10365"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNC16295.1"
                     /translation="AQLGPGEKAVTLPEGHTVTVGEVPVGSAGIYAQGGRASYPSTVV
                     MGCVTARVAGVDRVVLVAPPGPDGSIDPTTLAAAALCEVDEIYAVGGAQAIFALARGT
                     ETIRPVDVIAGPGNAWVQAAKREVFGEVAIDSLAGPSDLTVVVDSATNLHWAALDLCA
                     QAEHGEESPLVAIATEPGVIERLEAEVDAVAADNPTVKDCHLALVEAPSAADAIELVN
                     LIAPEHLELMSEGSAALAGAVRTAGCVFTGEETGTAFGDYVAGSNHVLPTDGTGRIFG
                     PLSPATFRRATARVSLDAESARKLAGPLDALARAEGLPVHGLSATARANDPDQETE"
     gene            997..>1183
                     /locus_tag="PLV77_10370"
     CDS             997..>1183
                     /locus_tag="PLV77_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HNC16296.1"
                     /translation="MSRQAKIERKTKETGIELSLDLDGSGYRNETGVGFFDHMLDLLA
                     RHANLGLEVKATGDLETG"
BASE COUNT          199 a          391 c          420 g          173 t
ORIGIN      
        1 ggcgcagctc gggcccggcg agaaggcagt gaccctgccc gaaggacaca ccgtcacagt
       61 cggcgaggtt ccggtcgggt ccgcgggcat ctatgcccag ggcgggcgtg cgtcctatcc
      121 ctcgacggtg gtcatgggct gcgtgaccgc gcgggtggcc ggggtggacc gggttgtgct
      181 ggtcgccccg cccgggccgg acggttcgat tgatccgacg accctggccg cggcggcact
      241 ttgcgaagtc gacgagatct acgcggtcgg cggcgcccag gcgatcttcg cgctcgcccg
      301 cggaaccgag acgatccgcc cggtcgacgt gatcgccgga ccgggcaacg cctgggtcca
      361 ggcggcaaaa cgggaggtgt tcggcgaggt cgcgatcgac tcgctggccg gaccatccga
      421 tctgaccgtc gtggtggatt cggcgaccaa cctgcactgg gcggcgctcg atctctgtgc
      481 ccaggccgag cacggagagg agagcccgct ggtcgcgata gccaccgaac caggtgtgat
      541 cgagcgcctg gaagccgagg tcgacgccgt tgcggcggac aacccgaccg tcaaggattg
      601 ccatctcgcc ctggtcgaag cgccttcggc cgcggacgcg atcgagttgg tcaatctgat
      661 cgcgcccgaa cacctcgagc taatgagcga gggctcggcc gccctggccg gggccgttcg
      721 caccgccggc tgcgtgttta ccggcgaaga gaccggtacc gccttcggcg attacgtggc
      781 cggctccaac cacgtgctgc ccaccgacgg caccggcagg attttcggac cgctttcgcc
      841 cgccaccttc agacgggcaa cggcgcgggt aagcttggac gccgaatcgg cccgcaaact
      901 ggccggcccg ctggacgccc tggcccgggc ggaggggctg ccggtccacg ggctctcggc
      961 gacggcccgg gcgaacgatc cagatcagga aacagaatga gcagacaggc gaaaatcgaa
     1021 cggaagacga aggagaccgg catcgagctg agtctcgatc tcgacggctc cgggtacagg
     1081 aatgaaaccg gggtcggttt cttcgatcac atgctggacc tgctcgcccg ccacgccaac
     1141 ctcggtctcg aggtcaaggc gactggggat ctggaaaccg gct
//
LOCUS       DAOQMF010000699         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_107799_length_1183_cov_2.943262, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000699 DAOQMF010000000
VERSION     DAOQMF010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107799_length_1183_cov_2.943262"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLV77_10375"
     CDS             <1..556
                     /locus_tag="PLV77_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16297.1"
                     /translation="LFLSLALWGFAKDDPGWSVLDGPAEETGKLLLPVILWFRGRFRL
                     PRQGYLLVLVSAATVAVMEGTEYAVQPEIYQPMRPVLEIMHPLLTGFVAAVAWQAAWH
                     RKSIFTAAAVGAWVLAMAAHSTNDVIVLSHHVDGSIARMTNFVSIAVIVLMYLLQKHS
                     ARQMVPPDKVGEVSPRWRPVAGPG"
     gene            complement(553..>1183)
                     /locus_tag="PLV77_10380"
     CDS             complement(553..>1183)
                     /locus_tag="PLV77_10380"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNC16298.1"
                     /translation="SDGGASLVKPIPDDWENVAVSELEDDGLTRCAWANGSQMMRTYH
                     DEEWGCPIVGEQDMFERLSLEAFQAGLSWATILRKRPAFREAFRDFDLEYCAGLTDAD
                     IEELMGNEGIIRARAKIEATRSNAAATIALREEGGLEGLIGSFVPERMPEPANQSEVP
                     TSSPESTNLARELRAHGFRFVGPTNMYALMEASGLVNTHLTSCFRRDQV"
BASE COUNT          179 a          388 c          399 g          217 t
ORIGIN      
        1 cctgtttctt tcgctcgccc tttgggggtt tgccaaggat gatccgggct ggtcggtgct
       61 ggacgggccg gccgaagaaa cgggcaagct gctgctgccg gtgatcctct ggttccgggg
      121 caggttccgg ctgccccgtc agggttacct gctggtgctg gtctcggccg cgacggtggc
      181 ggtgatggaa ggcaccgaat acgcggtcca gcccgagatc taccagccga tgcgtccggt
      241 cctggagatc atgcatccgc tgctgaccgg attcgtggcg gcggtcgcct ggcaggccgc
      301 ctggcacagg aagtcgatct tcactgcggc ggcagtcggg gcctgggtgc tggcgatggc
      361 cgcccacagc accaacgacg tgatcgtcct cagccaccac gtcgacgggt cgatcgcccg
      421 aatgaccaac ttcgtctcga tcgcggtgat cgtcctgatg tacctgctcc agaagcattc
      481 ggcccggcag atggtgccgc ccgacaaggt cggcgaggtc tcgccacgct ggcgaccggt
      541 cgcggggccc ggctagacct gatcgcgccg gaagcagctg gtcagatggg tgttgaccag
      601 gccgctcgcc tccatcagcg cgtacatgtt ggtcgggccg acgaacctga agccatgagc
      661 gcggagctcc ctggcgaggt tggtcgactc gggcgagctg gtcggaacct ccgactggtt
      721 cgccggttcg ggcatccgtt cgggcacgaa ggaaccgatc aggccctcaa gcccgccctc
      781 ctcgcgcagc gcgatcgtgg cggccgcatt cgaccgggtc gcttcgatct tggcccgggc
      841 ccggatgatc ccctcgttgc ccatcagctc ctcgatgtcg gcatcggtaa ggccggcgca
      901 gtattcgagg tcgaagtcgc ggaaagcctc ccggaaggcg gggcgcttgc gcaggatggt
      961 cgcccagctg agaccagcct ggaaggcctc gagcgagagc cgctcgaaca tgtcctgctc
     1021 gccgacgatc gggcagcccc actcctcgtc gtggtaggtc cgcatcatct ggctgccgtt
     1081 ggcccaggcg cagcgggtca gcccgtcatc ttcgagttcg ctcaccgcca cattctccca
     1141 gtcatcgggg ataggtttga ccagtgaagc accgccatct gat
//
LOCUS       DAOQMF010000700         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_108006_length_1182_cov_2.606034, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000700 DAOQMF010000000
VERSION     DAOQMF010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108006_length_1182_cov_2.606034"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLV77_10385"
     CDS             <1..275
                     /locus_tag="PLV77_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC16299.1"
                     /translation="LEVRELIDGHTSSADVDQTKPDPDLIEAALEKAGTRDALMVGDS
                     IWDVEAARRSGIDCLAVLTGGFSKAELAAAGAIEVRPSLADLEIPD"
     gene            284..>1182
                     /locus_tag="PLV77_10390"
     CDS             284..>1182
                     /locus_tag="PLV77_10390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC16300.1"
                     /translation="MAPEGTGSKEPVPLAGLVDEGQESPWLESGSEPFAPVSEDIRCD
                     VAVVGGGIVGVSTAYRLARAGCSVVLVEARRLAAGVTGHSSAKLSALQGLAYSAIADR
                     NGGEAASAYAELNARGIRFVEETTAALGIDCGLTHRPAVLYAETDEGRENLALEMDAA
                     LAAGLAVTDEDLAPLPFPVAATLTLENQAHFDPVKWVRGLAGALTELGGRVHETSRVT
                     GVDGLRGNRKVKMENGVTIEAERVVLATHVPILDRAAFYARVKPLTSFAVAGQIEGPL
                     PQGLYLSVDGPTRSISPLPGGGGT"
BASE COUNT          196 a          385 c          428 g          173 t
ORIGIN      
        1 agctcgaggt cagggaattg atcgacgggc acaccagttc cgccgacgtg gaccagacca
       61 agcccgatcc ggacctgatc gaagcggccc tggagaaggc ggggacccgt gacgccctga
      121 tggttggcga ttcgatctgg gacgtggagg cggcccggcg ctcggggatc gactgcctcg
      181 ccgtcttgac gggcggcttc tcgaaggcgg aactcgcggc ggccggggca atcgaggtaa
      241 ggccgtccct ggccgatctg gaaatcccgg actgaccaac gccatggcgc ccgaaggcac
      301 aggaagtaaa gagccggtgc cgctcgcggg cctggtggac gagggccagg aatcgccctg
      361 gctggaaagc gggtcggagc cgtttgcccc ggtcagcgaa gacatccgct gcgacgtggc
      421 ggtggtgggt ggcggaatcg tcggggtctc gacggcttac cggctcgccc gggcgggatg
      481 ctcggtggtc ctggtcgagg cgcgacggct cgccgccggt gtgaccggtc actcgtcggc
      541 caagctgagc gcgcttcagg gtctggccta ctcggcaatc gctgacagga acgggggcga
      601 agcggcctcc gcctatgccg aactgaacgc ccggggcatc cgcttcgtcg aggagacgac
      661 cgcggcactg ggaatcgact gcgggctcac ccaccggccc gcggttttgt acgccgaaac
      721 cgatgaagga cgggagaacc tggccctgga gatggacgcg gcgctggccg ccggcctcgc
      781 ggtgaccgac gaggatctgg cgcccttgcc gtttccggtt gccgcgacgc tcaccctcga
      841 aaaccaggcc cacttcgacc cggtcaaatg ggtcaggggt ctcgccggcg ccctgaccga
      901 gctcggcggc cgggttcatg aaacaagccg ggtgaccggg gtcgacggat tgcggggcaa
      961 ccgcaaggtc aaaatggaaa acggcgtgac gatcgaggcc gaacgggtgg tgcttgccac
     1021 acacgtaccg attctcgacc gcgccgcctt ctacgcccgg gtcaagcccc tgacctcctt
     1081 cgcggtcgcc ggccagatcg aggggccgct cccgcagggt ctctacctga gtgtcgacgg
     1141 cccgacccgc tcgatcagcc ccctgcccgg cgggggaggt ac
//
LOCUS       DAOQMF010000701         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_108251_length_1180_cov_4.639111, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000701 DAOQMF010000000
VERSION     DAOQMF010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108251_length_1180_cov_4.639111"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>1180
                     /locus_tag="PLV77_10395"
     CDS             114..>1180
                     /locus_tag="PLV77_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNC16301.1"
                     /translation="MSLPTEEQVKEKLTAVIDPELRRNIVELGMVRSVEIKDDGQINV
                     TVSLTTPGCPIRSHFQEAVVQQVSSLDGVTGVGVGFDVLSDEEKSGLRETLGRGKLPE
                     GALAQVKNVICVASGKGGVGKSTMTANLAAALQAEGKKAGALDCDVYGYSIPRMLGVD
                     QKPEVNSDRKILPPVAESGVKTMSIGYFVEQNSAVVWRGPMLHKAIQQFLEDVAWGEL
                     DYLLLDLPPGTGDIAMTLAQLLPQAKITIVTTPQPAAQSVARRSAEMAAKVDLEVMGI
                     IENMAGFTTPEGETFSIFGEGGGQLLADELEVPLFGSVPLSEELREHADVGNPLSVDQ
                     PDSPAGQAIVEIARKIIATSP"
BASE COUNT          212 a          408 c          371 g          189 t
ORIGIN      
        1 gtcatatgcc tgactaaggc gccgaccggt gaaactcgcc ccccgcccgc ctttccgctc
       61 gcccggtccg ccctccgccg cggcagaacg ggcggctctg cgagaatcag ggcatgtccc
      121 tcccgaccga agagcaggta aaagagaagc tgaccgcggt catcgacccg gagcttcgtc
      181 gcaacatcgt cgaactcggc atggtccgca gcgtcgaaat caaggatgac ggccagatca
      241 acgtgaccgt ctcgttgacc actccgggct gtccgatccg ctcccatttc caggaagcgg
      301 tggtccagca ggtctcctcg cttgacgggg tgaccggggt cggagtcggc ttcgatgtgc
      361 tgtcagatga agagaagtca ggcctgcgcg agacgctcgg ccggggcaag ctccccgagg
      421 gcgcgctggc ccaggtcaag aacgtgatct gcgtcgcctc cggcaagggc ggggtcggca
      481 agtcaaccat gaccgcgaac ctggccgccg cgcttcaggc cgaaggcaag aaggccggtg
      541 ccctcgactg cgacgtctac ggctactcga tcccccgcat gctcggggtc gatcagaagc
      601 ccgaggtcaa ttccgaccgc aagatccttc ctccggtcgc cgaaagcggc gtcaagacga
      661 tgtcgatcgg ctacttcgtc gagcagaact ccgcggtggt ctggcgcggt ccgatgctcc
      721 acaaggcgat tcagcagttc ctcgaagacg tcgcctgggg tgagctcgac tacctgctgc
      781 tggacctgcc gcccggcacc ggtgacatcg ccatgaccct ggcccagctc ctgccgcagg
      841 cgaagatcac gatcgtcacc acgccgcagc cggccgcgca gtcggtcgcc cggcgttcgg
      901 ccgagatggc cgccaaggtc gacctcgagg tgatgggaat catcgagaac atggccggct
      961 tcaccacccc ggagggcgag accttctcga tcttcggcga aggcggcggt cagctcctgg
     1021 ccgacgaact cgaggtgccg ctgttcggat cggtgccgct ctcggaagag ctccgcgagc
     1081 acgccgatgt cggcaacccc ctctcggtcg atcagccgga ttccccggcc ggtcaggcga
     1141 tcgtcgaaat cgcccgcaag atcatcgcca cctcacccat
//
LOCUS       DAOQMF010000702         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_108577_length_1179_cov_2.030249, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000702 DAOQMF010000000
VERSION     DAOQMF010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108577_length_1179_cov_2.030249"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLV77_10400"
     CDS             complement(<1..621)
                     /locus_tag="PLV77_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16302.1"
                     /translation="MQTRLPQTSSGRARGFSLAAALIVAAFVIALASWARPADALTVD
                     QTQSKIDAINSSVDDLKTQVAADNRKIDALIGELSGLRARASELEAQLAAKQAELDKV
                     EAALEREKEHLAQVRARLHRALKILEEQLVAVYMNGSPDVEDMVLASSDFGDLVSSAG
                     YADAIQDRNESVITRVKDLRDQIEAIVQRMKVQEKKLQAARDEIAAE"
     gene            complement(732..>1179)
                     /locus_tag="PLV77_10405"
     CDS             complement(732..>1179)
                     /locus_tag="PLV77_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alkanal monooxygenase"
                     /protein_id="HNC16303.1"
                     /translation="GMPYSFAHHFSAGNTEAAVQLYRSRFEPSAFLEEPKVMIGVGVI
                     CAETDEEARWLAGPSRLAFARLRSGRPGRFPTPEEAAAHDFTPDEEAVVASVAKGHIF
                     GSPETVQAGLVDLIERTGADELIISTMVYEQKDRLRSYELVSGIAL"
BASE COUNT          204 a          358 c          409 g          208 t
ORIGIN      
        1 ttcggccgcg atctcgtcgc gggcggcctg aagtttcttc tcctggacct tcatccgctg
       61 gacgatcgcc tcgatctggt cacggaggtc cttgacccgg gtgatcaccg attcgttgcg
      121 gtcctggatc gcgtcggcgt atccggcgct ggaaacgaga tcgccaaagt ccgaggaagc
      181 gagaaccatg tcctcgacat ctggcgaacc gttcatgtaa acggcgacaa gctgctcttc
      241 gaggatcttc agggcccggt gaagccgggc gcgaacctgg gcgaggtgtt ccttctcacg
      301 ctcgagcgca gcctccacct tgtcgagttc ggcctgcttg gcggccagct gggcctcaag
      361 ctcggaggcc cgggcgcgca gcccggaaag ctcgccaatc agggcgtcga tcttgcggtt
      421 gtcggcggcg acctgggtct tcaggtcgtc gaccgagctg ttgatcgcgt cgatcttcga
      481 ctgggtctgg tcaaccgtca gcgcgtcggc cgggcgtgcc caggaggcaa gcgcgatcac
      541 gaaagcagcc acgatcaggg ccgcagcaag gctgaagccg cgggcacggc cgctggatgt
      601 ttgcgggagt ctggtctgca tgagggtccg ccggaaggtg caggcggaag aagcaaggta
      661 gcgggagaaa gcccggcgcc ggaggtcccg acggtccgtg ggaaataccg tgcaattgaa
      721 gggaaacggc tctacagcgc gatgccggag acgagttcgt aggacctgag ccggtccttt
      781 tgctcgtaaa ccatggtcga gatgatcagc tcgtcggctc cggtccgctc gatcaggtcg
      841 accagtccgg cctgcacggt ttcggggctg ccgaatatat ggcccttggc cacggaagcg
      901 acgactgctt cctcatccgg ggtgaaatca tgcgccgccg cctcttcggg agtggggaac
      961 cggcctggcc ggccgctccg gagccgggcg aaagcaagcc gcgagggacc ggccagccag
     1021 cgggcttcct cgtcggtttc ggcgcagatc acccccaccc cgatcatcac cttcggttct
     1081 tccagaaagg cggacggctc gaagcgggag cggtagagct gaaccgcggc ctcggtgttg
     1141 ccggcgctga agtgatgggc gaaggaatag ggcatgccg
//
LOCUS       DAOQMF010000703         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_108671_length_1178_cov_3.264470, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000703 DAOQMF010000000
VERSION     DAOQMF010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108671_length_1178_cov_3.264470"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLV77_10410"
     CDS             complement(<1..942)
                     /locus_tag="PLV77_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC16304.1"
                     /translation="MPEKPTVKSPPRDPLAPFSDDVRRWFSASFEGATPAQALGWQSI
                     SSGSDTLICAPTGSGKTLASFLWGIDRLARSHRAVAAGEAPEPEPGVRLVYVSPLKAL
                     SYDIERNLRAPLKGIGVDLSVGIRTGDTSQTERNRMRREPPEILITTPESLYLMLSSG
                     ARDILTSVETVIVDEIHAVAATKRGAHLALTLERLEHLVQENAGHGVQRIGLSATQRP
                     LERIAGFLSGPGRECEIVDPGQRKELDLEIVVPVEDMTAPEADQHDDGSADDAPIRSI
                     WPAIYPELLEQVRAHTSTIVFVNNRRAAERLAQRLNDL"
BASE COUNT          204 a          362 c          410 g          202 t
ORIGIN      
        1 gaggtcgttc agccgctggg cgaggcgctc ggcggcacgg cggttgttga cgaagacgat
       61 ggtcgaggtg tgagcgcgga cctgctcgag cagctccgga tagatcgccg gccagatcga
      121 ccggatcggg gcatcgtccg cggagccatc gtcgtgctgg tcggcttcgg gtgcggtcat
      181 gtcctcgacc ggcaccacga tttccaggtc gagttccttg cgctggcccg ggtcaacgat
      241 ttcgcattcg cgaccggggc cggaaaggaa gccggcgatg cgctcgaggg gccgctgggt
      301 ggccgagagg ccgatcctct gcaccccgtg gccggcgttt tcctgaacga ggtgttccag
      361 ccgctcaagg gtcagggcga ggtgggcgcc gcgcttggtc gcggcaacag cgtgaatctc
      421 atcgacgatc acggtctcga ccgaggtgag gatgtcccgg gcgcccgagg aaagcatcag
      481 gtagagagac tccggggtgg tgatcaggat ctccggcggt tcgcgccgca tccggttgcg
      541 ttcggtctgg gaagtgtccc cggtccggat cccgaccgaa aggtccaccc cgatcccctt
      601 cagcggggcg cgcaggttgc gctcgatgtc gtaggaaagc gccttgagcg gcgatacata
      661 gaccagcctc acccccggtt cgggctcggg cgcttccccg gccgcgacgg cccggtggct
      721 gcgggcgagc cggtcgatcc cccagaggaa gctggccagg gtcttgccgg agccggtcgg
      781 agcgcagatc aacgtgtccg agccggagga gatcgactgc cagccgagcg cctgggccgg
      841 ggtcgcgcct tcgaacgaag ccgaaaacca gcgtcggaca tcgtcgctga agggagcgag
      901 cgggtcccgg ggaggtgatt tcacggtcgg cttttcaggc atcgctaaac ccctgatgct
      961 acgcccccgc ccacctattt cgtgtgtgac ccgccgcagc cgaaagaacc tcagagatgg
     1021 gatctgcaaa aatgtgccaa aacagcgcac ctttgcggtc tgtccgtggt tcttgggaaa
     1081 gaagtccagt caagtttcca gtcccgtctc gatccaacgg gctcaccaga aaggtgaagt
     1141 gagtcgcagc catgtcaaag agtgattcag gctccccc
//
LOCUS       DAOQMF010000704         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_108848_length_1177_cov_3.684492, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000704 DAOQMF010000000
VERSION     DAOQMF010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108848_length_1177_cov_3.684492"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLV77_10415"
     CDS             complement(<1..768)
                     /locus_tag="PLV77_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNC16305.1"
                     /translation="MILAIDQGTTGSTALVFNDEGRICGRGYAEFRQHFPKPGWVEHD
                     AEEIWETSWGVAARALAGAGISGPDLDAIGITNQRETVVAWDRETGKPLHHALVWQDR
                     RGAARCDELKAVGLEGLVRERTGLVLDPYFSATKIEWLRQNVEGADRAVFGTIDSWLV
                     HRMCGEHLTDLTNASRTMLFDTGRLQWDPELCEVFGTDPASLPEARPSAGMFGTTSGF
                     GGEVPVAGIAGDQQAALFGQMCHEPGMAKNTYGTGSFV"
     gene            complement(765..>1177)
                     /locus_tag="PLV77_10420"
     CDS             complement(765..>1177)
                     /locus_tag="PLV77_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16306.1"
                     /translation="DLAVAMFAKRVGGGFGFALGFSAWVIRRRCGAEPQAPISGQRAM
                     KLDVLRRVLPFAPAYGMETGMTIDAVRAGYRLGEYEIDLAHKATGKSIKGFIHRFRQL
                     LDFARVWWARRGGRGPDGKRPVESVAGSRPGATG"
BASE COUNT          180 a          433 c          372 g          192 t
ORIGIN      
        1 aacgaagctg ccggtgccgt aggtgttctt cgccatgccc ggctcgtgac acatctgccc
       61 gaacagggca gcctgctggt caccggcgat cccggccacc ggcacctcac cgccgaagcc
      121 cgaggtcgtg ccaaacattc ccgccgaggg ccgggcctcg ggcagcgagg cagggtcggt
      181 cccgaatacc tcgcagagct ccgggtccca ctggagcctg ccggtatcga acagcatggt
      241 ccgcgaggcg ttggtcaggt cggtcaggtg ctcgccacac atccggtgga ccagccagga
      301 gtcgatcgtt ccgaagaccg cccggtccgc gccttcgacg ttctggcgca gccactcgat
      361 cttggtcgcc gagaaataag ggtcgaggac cagcccggtc cgttcgcgaa ccagtccctc
      421 cagccccacc gccttcagct catcgcagcg ggcagcgccc cggcggtcct gccagaccag
      481 ggcgtgatgc agcggcttgc cggtctcccg gtcccaggca accaccgtct cgcgctggtt
      541 ggtgatcccg atcgcgtcga ggtcgggacc gctgatcccg gccccggcca gagcccgtgc
      601 cgccaccccc cagctggtct cccagatctc ctccgcatcg tgctcgaccc aacccggctt
      661 tgggaagtgc tgccggaatt cggcgtagcc gcgaccgcag atccggccct cgtcgttgaa
      721 gaccagcgcg gtcgaaccgg tggtcccctg atcgatcgcg aggatcatcc tgtcgctccg
      781 ggcctgctcc cggcgacact ttcaaccggt cgcttcccgt ccggtccgcg gccaccgcgc
      841 ctggcccacc agacccgggc gaaatcgagg agttggcgga accggtggat gaaccccttg
      901 atgctctttc cggtcgcctt gtgggcgagg tctatctcgt actcgccgag ccggtagccg
      961 gcccggaccg cgtcgatggt cattccggtc tccattccgt aggcgggagc gaaggggagt
     1021 acccgtcgca gcacgtccag tttcatcgcc cgctgacccg agatcggcgc ctgtggttcg
     1081 gccccgcagc gtcggcggat cacccaggcc gagaatccga gggcgaagcc gaagccgcca
     1141 ccgacccttt tggcgaacat cgctaccgcg aggtcgc
//
LOCUS       DAOQMF010000705         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_108861_length_1177_cov_3.408200, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000705 DAOQMF010000000
VERSION     DAOQMF010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108861_length_1177_cov_3.408200"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PLV77_10425"
     CDS             complement(<1..1167)
                     /locus_tag="PLV77_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNC16307.1"
                     /translation="MGKLGGFLQIERAGIPYRDPAERVGDYNEFVVRRSDEELADQGA
                     RCMECGVPFCHNGCPLGNLIPDWNDLVYHGRWQDAIRQLHSTNNFPEFTGRLCPAPCE
                     AACVLEIREGEAVTIKQIEQSIADRAWKEGWIRPRPPKRETGRHVAVVGSGPAGMAAA
                     QQLRRGGHSVTLFERDEAAGGLVRFGVPDFKIEKTVVERRVQQLIHEGVEVRCGVEVG
                     VDVTVEELKERFDAVVLATGSRVPRDLPAPGRENDGVHFAMDYLYSRNRWLASEVGPK
                     PMTEAPAPAPISAKGKHVVVIGGGDTGADCVGHSIREGAAEVVQLELLPEPPEHRPDD
                     RTPWPLWPTKFRLSYAMEEAKGIEKGEQDFSVTTTSFNGEDGQVRSITVAKADPN"
BASE COUNT          180 a          391 c          396 g          210 t
ORIGIN      
        1 gttcggatcg gccttggcga ccgtgatcga ccggacctgg ccgtcctccc cgttgaagga
       61 ggtcgtggtc acggagaagt cctgttcgcc cttttcgatt cccttcgctt cttccatcgc
      121 gtaggagagg cggaacttgg tcggccagag cggccacggg gtgcggtcat ccgggcgatg
      181 ctcgggcggc tcgggcagca gttcgagctg gaccacctcg gccgcgccct cgcggatcga
      241 atgcccgacg cagtcggcgc cggtgtcgcc accgccgatc accacgacat gcttgccctt
      301 cgccgaaatc ggggccggtg ccggggcctc ggtcatcggc ttgggaccga cctcggacgc
      361 cagccagcga ttgcggctgt agagatagtc catcgcgaaa tggacaccgt cgttttcgcg
      421 gcccggcgcc ggaaggtcac ggggaacgcg ggaaccggtg gcaagcacga ccgcatcgaa
      481 gcgttccttc agttcctcga cggtcacatc gaccccgact tcgaccccgc acctgacctc
      541 cacgccttcg tggatcaact gctggacgcg gcgttcaacc acggtctttt cgatcttgaa
      601 gtcgggtact ccgaagcgga ccaggccgcc ggcggcttca tcccgctcga agagggtgac
      661 gctgtggccg ccccggcgaa gctgctgggc ggcggccatg ccggccgggc ccgaaccgac
      721 cacggcgacg tggcggccgg tctcgcgctt gggcggacga ggccggatcc agccttcctt
      781 ccaggcccgg tcggcgatcg actgctcgat ctgcttgatc gttaccgctt cgccctcgcg
      841 gatctcgagc acgcaggcgg cttcgcaggg ggccgggcaa aggcgaccgg tgaactcggg
      901 gaagttattg gtcgagtgga gctgacggat cgcgtcctgc cagcgtccgt ggtagacgag
      961 gtcgttccag tccgggatca ggttgccgag cgggcagccg ttgtggcaga aggggacacc
     1021 acattccatg cagcgcgcgc cctggtcggc cagctcctca tcggatctgc gaaccacgaa
     1081 ctcgttgtag tcgccgaccc tttcggcggg atcccggtag gggattccgg cacgttcgat
     1141 ctggaggaaa cctcccagct tgcccattgc tcaggct
//
LOCUS       DAOQMF010000706         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_109487_length_1174_cov_2.458445, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000706 DAOQMF010000000
VERSION     DAOQMF010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109487_length_1174_cov_2.458445"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..514
                     /locus_tag="PLV77_10430"
     CDS             29..514
                     /locus_tag="PLV77_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16308.1"
                     /translation="MGAPARTIPVLPARVPSRRTVPHRRPATRRRPGSRQHSGLRLIG
                     RSAHAVTHFPESGLVRTAATGRRWIFVIGALLLAIVAINVVTVSYGAMASKLETQIES
                     IERQNAILKSSQTAALAMPRVQAQAEAEGMYLPEPDAIHYRSFQNSDYAAAAERLAAG
                     G"
     gene            514..>1174
                     /locus_tag="PLV77_10435"
     CDS             514..>1174
                     /locus_tag="PLV77_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16309.1"
                     /translation="MRLQRPERRIGLLFAVSLVLLLGLFARAFLLQVVQGSSLASQAK
                     GQQEDIIEVPGLRGSILDRNGQPLAGSEAGATIYATPYQIKDPPEEARQVAKALDADP
                     GEVLDAITQPGGFSYVDRKVDLKKAEAVDKLGLEGIGQVPDTLRVRPQGDIASQVIGA
                     VSDEGEGLTGIEAGQDGVLKGVNGERRLVKDALGDPISLDNVRDAEDGQPVQLTLDSN
                     IQ"
BASE COUNT          200 a          408 c          390 g          176 t
ORIGIN      
        1 atgagaatcc cgccggggag gttcgctgat gggcgccccg gctcgcacca tcccggtcct
       61 tcccgctcgc gttccctccc ggcgcacggt ccctcaccgt cggcccgcca cccggcgccg
      121 gcccggcagc cggcagcact ccggcctgcg cctgattggc aggagcgccc atgcggtcac
      181 tcatttcccc gagtcggggc tggttcgcac cgccgccacc ggccgacgct ggatcttcgt
      241 gatcggcgcg ctgctactgg cgatcgtcgc gatcaacgtg gtcaccgtca gctacggcgc
      301 gatggccagc aagctcgaaa cccagatcga atcgatcgag cggcaaaacg ccatcctgaa
      361 gtcgtcgcag acggctgctc tggcgatgcc ccgcgttcag gcccaggctg aagccgaagg
      421 catgtacctg ccagagcccg acgcgattca ctaccgctcc ttccagaact ccgactatgc
      481 ggcggccgcc gagcgactgg cggccggggg ctgatgcgac tgcaacggcc cgaacggcgg
      541 atcggcctgc tcttcgcggt cagtctggtt cttctgctcg ggctcttcgc ccgggctttt
      601 ctgcttcagg tggttcaggg ttcctcgctc gcatcccagg cgaaaggcca gcaggaagac
      661 atcatcgagg tgcccggtct gcgcggctcg atcctcgacc gcaacggcca gccgctggcc
      721 gggtccgaag ccggcgccac gatttacgcc accccgtacc agatcaagga tccgccggag
      781 gaggccaggc aggtcgcgaa ggcgctcgac gccgatccgg gcgaggtgct tgatgcgatc
      841 acccagcccg gcgggttctc atacgtcgat cgcaaggtgg acctgaaaaa ggccgaagcg
      901 gtcgacaagc tgggcctcga agggatcgga caggttccgg acaccctgcg ggtccggccc
      961 cagggtgaca tcgcctccca ggtgatcggg gcggtcagcg acgaaggcga gggactgacc
     1021 ggaatcgaag ccggacagga cggcgtgctc aagggcgtaa acggggagcg gcggctggtc
     1081 aaggacgcac tcggcgaccc gatcagcctc gacaacgtcc gcgacgccga ggacggccag
     1141 ccggttcagc tgacgctgga ctcgaacatc cagg
//
LOCUS       DAOQMF010000707         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_110343_length_1169_cov_4.097846, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000707 DAOQMF010000000
VERSION     DAOQMF010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110343_length_1169_cov_4.097846"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1169
                     /locus_tag="PLV77_10440"
     CDS             23..>1169
                     /locus_tag="PLV77_10440"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HNC16310.1"
                     /translation="MDTYAALKTAYEMGQLNEGSAGYASTPAIDNRQYWIETVPNGHQ
                     IIHALSMQQRLEESTGAPVPHFIWTSLYGPGDGDGNDDPTVPNNVTWGILGDWVDAIQ
                     ADTSDTDPQAKAVAHAPAAATDRCFNDDGSVLAIEPQTLNSGICGSTFPSHETPRMIA
                     GGPIANDVLKCALKPVDPSDYDGKLDAGQLAELESIFPTGVCDWEEPGVEQTYNTRTW
                     RAWPTGQADNSAPSTTISSSPRPSNAPATVDFEFSSSETGSQFECRLDDGGNTAGYED
                     CASPMRYGPLGDDLYTFSVRATDAAGNTGGPATVKFRTGGPAPVLAISPAVFAFGPIN
                     VGGELGEPGEFTLSNPGSDPVNLDDGTLTGPDAGSFKLLPNGSTCYFL"
BASE COUNT          228 a          419 c          354 g          168 t
ORIGIN      
        1 ggaaaccatg cagcaatggc agatggatac gtacgccgcg ctgaagaccg cctacgagat
       61 gggccagctg aacgagggct cggccggata cgcctcgact cccgccatcg acaaccggca
      121 gtactggatc gaaacggtgc ccaacggcca ccagatcatc cacgcgctct cgatgcagca
      181 aaggctggaa gagtcgaccg gtgcgccggt tccgcatttc atctggacca gcctctacgg
      241 cccgggggac ggcgacggaa atgacgaccc gaccgtgccg aacaacgtga cctgggggat
      301 cctcggcgac tgggttgacg cgattcaggc cgacacctcg gacaccgacc cccaggccaa
      361 ggctgtcgcc catgccccgg ccgccgccac cgaccgttgc ttcaacgatg acggcagcgt
      421 gctggcgatc gaaccccaga ccctgaactc gggcatctgc gggtcaacct tccccagcca
      481 tgaaacgcca aggatgatcg ccggcggtcc gatcgcgaac gacgtcctca aatgcgcgct
      541 gaagccggtc gacccgtcgg attacgacgg caagctcgac gccggccagc tcgcagagct
      601 cgagagcatc ttcccgaccg gggtctgtga ctgggaagaa cccggggtcg agcagaccta
      661 caacacccgg acctggcggg cctggccgac cggacaggcc gacaacagcg ctccctcaac
      721 cacgatctct tcgtcgccgc ggccatcgaa tgccccggcc acagtcgact tcgagttctc
      781 gtcaagcgag accggttcgc agttcgagtg ccgcctggat gacggcggca ataccgccgg
      841 ctacgaagac tgcgcctcac cgatgcgtta cggcccgctc ggggacgacc tctacacctt
      901 ctctgtccgg gcgacggacg ccgccggaaa caccggcggt ccggccacgg tgaagttccg
      961 caccggtggc ccggctccgg tgctcgccat cagcccggcc gtgttcgcct tcggcccgat
     1021 caacgtcggc ggtgagctgg gagagccggg tgaattcacg ctcagcaacc cgggaagcga
     1081 tcccgtcaac ctagatgacg ggaccctcac cggacccgac gccggttcct tcaagctcct
     1141 gcccaacggc agcacctgct acttcctcg
//
LOCUS       DAOQMF010000708         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_110523_length_1168_cov_4.241689, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000708 DAOQMF010000000
VERSION     DAOQMF010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110523_length_1168_cov_4.241689"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLV77_10445"
     CDS             <1..790
                     /locus_tag="PLV77_10445"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNC16311.1"
                     /translation="PWARRHRVLRWPIIRGIVALAESLKIGFRALGMSANAQLDEGEE
                     EEELGGMAWGMTIFVSLAFAILLFFVIPVGLTSLIKDWLDSSFLFWLVEGIVRTAIFI
                     GYIVMISRLEDLRRVFEYHGAEHKTISCYEAEDELIPSRAALYSRLHPRCGTSFLLIV
                     MVVAIFVFAPLGLPAWYWLVISRIVGVPLIAGIAYELIKWAGRNRDKSWVRAVMWPGL
                     MLQNLTTREPDEEQLEVAIAALNAVLEVEKPEENAYLEPIEIVA"
     gene            914..>1168
                     /locus_tag="PLV77_10450"
     CDS             914..>1168
                     /locus_tag="PLV77_10450"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNC16312.1"
                     /translation="MAEQIEARFAELEQQMSDPEVIGDRERYAEVGREYRQIQPANAL
                     VTEYRTLSDDLEGADELLEEGEDEDLEKVVREAPARLEELE"
BASE COUNT          200 a          355 c          385 g          228 t
ORIGIN      
        1 accctgggcc cgccggcacc gggtattgcg ctggccgatc atccgcggca tcgtcgccct
       61 ggccgaatcg ctgaagatcg gatttcgggc cctgggcatg tcggccaacg cccagctcga
      121 cgagggagag gaggaagagg aacttggcgg catggcctgg gggatgacga tcttcgtctc
      181 gcttgccttc gcgatcctgc tcttcttcgt gattcccgtc gggttgacca gcctgatcaa
      241 ggactggctt gactcttcct ttctcttctg gctggtcgaa gggatcgtcc ggaccgcgat
      301 cttcatcggt tacatcgtga tgatctcccg gctggaggat ctgcggcggg tcttcgagta
      361 ccacggggcc gagcacaaga cgatctcctg ctacgaagcc gaggacgaac tgattccttc
      421 ccgggccgcc ctctactcgc ggctccatcc gcgttgcggc accagcttcc tgttgatcgt
      481 catggtcgtc gcgatcttcg tcttcgctcc gctgggcctg cccgcctggt actggctggt
      541 gatttcgcgg atcgtcgggg tgccactgat cgccggcatc gcctacgagt tgatcaagtg
      601 ggccgggcgc aaccgggata agtcctgggt gcgggcggtc atgtggcccg gcctgatgct
      661 gcagaacctg accacccgcg agccggacga agagcagctg gaagtcgcga tcgccgccct
      721 gaacgccgtc ctcgaggtcg aaaagcccga agagaacgcg tacctggaac cgatcgaaat
      781 cgtggcgtga ccgcagtcgg gccagtcttc gccggccctc cagattgaac ccgccccttc
      841 ctctcacatt cactgacggt aggaaggggc tctgctgtcg gttattcggg acaatggcct
      901 tgtgattcag aagttggcgg aacagattga ggctcgcttt gccgagcttg agcagcagat
      961 gagcgacccc gaggtgatcg gggaccgcga gcggtatgcc gaggtcggtc gcgagtaccg
     1021 ccagattcag ccggccaatg cgctggtcac cgagtaccgg accctgagcg acgatctcga
     1081 gggggccgac gaactgctcg aggagggcga ggacgaggat ctcgaaaagg tggttcgtga
     1141 agccccggcc cggctggaag agctcgag
//
LOCUS       DAOQMF010000709         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_110720_length_1167_cov_3.789568, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000709 DAOQMF010000000
VERSION     DAOQMF010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110720_length_1167_cov_3.789568"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLV77_10455"
     CDS             complement(<1..938)
                     /locus_tag="PLV77_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC16313.1"
                     /translation="MSESQHVASLTEATALFEGDPGSLRVLTSAELPVLRGISIKRLL
                     LAPGSIREPHWHANASELTYCVKGDLLVSVLDDADGFSSFTISAGEMFYVASGSLHHI
                     ENLGSGEAELILAFSHERPEDFSMHAAFGAMSDAVLGNTYDLPAADFAVISRNTEPVY
                     IVSREGRPSVPEHSSFDDPHKFAIEGQAAPIDSPAGSAKLARKQFWPIMEDLSMYSLR
                     VTPDGMREPHWHPVTAELGYVVKGEARMTVMDPDGSTDTYGLGPGDVYFIPPAYPHHI
                     EDTGQDDFHFLVFFDRHTPKDIGYRASASAISREVLA"
     gene            982..>1167
                     /locus_tag="PLV77_10460"
     CDS             982..>1167
                     /locus_tag="PLV77_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16314.1"
                     /translation="MFRGEGGLRRAAAGAIVTAAALGLLAPAASAGTVRKPAGTPREL
                     RANAGGGVAANVNMTNSR"
BASE COUNT          211 a          364 c          405 g          187 t
ORIGIN      
        1 gccagcacct cccgcgaaat ggccgaagcc gaagcccggt agccgatgtc cttcggggtg
       61 tgccggtcaa agaaaacgag gaagtggaag tcgtcctgac cggtgtcctc gatgtggtga
      121 gggtaggcgg gagggatgaa gtagacatcg ccgggcccga gcccgtaggt atcggtcgaa
      181 ccgtccggat ccatcaccgt catcctggcc tcgcccttga ccacgtaccc cagctcggcg
      241 gtgaccggat gccaatgcgg ttccctcatt ccgtccgggg tgacccgcag cgagtacatc
      301 gagaggtcct ccatgatcgg ccagaactgc ttgcgggcca gcttcgccga gccggccggg
      361 gagtcgatcg gggcagcctg cccttcgatc gcgaacttgt gtggatcgtc gaatgaggaa
      421 tgctccggaa ccgacggccg gccctcgcgg ctgacgatgt aaaccggctc ggtattgcgg
      481 gaaatcacgg cgaaatccgc ggcaggcagg tcgtaggtgt tgccgagtac ggcgtcactc
      541 atcgctccga aggcggcgtg catcgaaaag tcttcgggtc gctcgtgact gaacgccagg
      601 atcaactcgg cctcgccgga acccaggttc tcgatgtggt gcaggctgcc gctggccacg
      661 tagaacatct ccccggccga aatcgtgaac gaggagaagc cgtcggcgtc gtcgaggacc
      721 gagaccagca ggtctccctt gacgcagtag gtcaattcgc tggcgttggc gtgccagtgc
      781 ggttcccgga tcgaacccgg ggcgaggagc agacgcttga tcgagatccc ccgcaaaacc
      841 ggcagctcgg ccgaagtcag aacccggagc gagccggggt cgccctcgaa cagggccgta
      901 gcttcggtca gggaagcaac atgctgactc tcgctcatgt ccggcgaccc tagcagcggg
      961 gatcgggggt aggatcgaag gatgtttcgg ggagaggggg gtcttcgcag agctgccgct
     1021 ggcgcgatcg tcactgccgc cgccctgggc ctgctcgccc cggcggcgtc cgccggcacg
     1081 gtccggaaac cggccgggac gccgcgcgag ttgcgggcca atgccggtgg cggggtcgcc
     1141 gcgaacgtca acatgaccaa ctcccgg
//
LOCUS       DAOQMF010000710         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_111155_length_1165_cov_2.394595, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000710 DAOQMF010000000
VERSION     DAOQMF010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111155_length_1165_cov_2.394595"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLV77_10465"
     CDS             <1..830
                     /locus_tag="PLV77_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC16315.1"
                     /translation="AEDALSLAKLIAERTGAELVLASVIPNPVGGSFGPALPAEAFTE
                     LAGKARASLEAAAAGVGAGVEVEQSSSAAHGLQAIAERISADLIVVGSSPSAETGHTR
                     AGRKARQLLSGADCAIAVAPDGFHDRAGLDRIGVGIDGSPESRLATETAVGLAPDGSE
                     LQLLAVAAGFADEWGRWGVTYPLAEMAEATRESADALLKEMEQVVPARLTAKKLLLEG
                     TAPIQLAEASAGLDLLCLGSRGYGPVRRVLLGSVSSDVMNHAASAVLVVPRTDQTND"
     gene            complement(985..>1165)
                     /locus_tag="PLV77_10470"
     CDS             complement(985..>1165)
                     /locus_tag="PLV77_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16316.1"
                     /translation="PNMRVYDWANKVKDEWFIDDGIHFTSEGYKVRAQAIANAVPKAF
                     GIWSSFWNQSGCLIE"
BASE COUNT          187 a          389 c          402 g          187 t
ORIGIN      
        1 acgcagaaga tgccctttcc ctcgcgaagc tgatcgccga aaggaccggc gccgaactgg
       61 tcctcgcctc ggtcattccg aatccggtcg gtggcagttt cggtccggcg ctcccggccg
      121 aggccttcac cgagctggcc ggcaaagcca gggccagcct cgaggcggcc gctgccgggg
      181 tcggtgccgg cgttgaggtc gagcagtcct cctcggcagc ccatgggctt caggcaatcg
      241 ccgaacggat cagcgcagac ctgatcgtgg tcggttccag ccccagcgcg gaaaccggcc
      301 atacccgggc cggccgaaag gcgaggcagc tcctttccgg cgccgactgc gcgattgctg
      361 tcgccccgga cgggtttcac gaccgggccg gtctcgatcg aatcggagtc ggcatcgacg
      421 gttcgccgga gtcaaggctg gcgaccgaga cggccgtcgg gctcgcgccg gatggctcgg
      481 aacttcagct gctggcggtg gccgccggtt tcgccgacga atggggtcgc tggggagtca
      541 cctacccgct ggctgaaatg gcggaagcga cccgcgagtc cgccgacgct ctgctcaagg
      601 aaatggaaca ggtggttccg gcccgactca ccgcgaagaa gctgctgctt gaaggaaccg
      661 cgccgatcca gctggccgag gcctcggccg ggcttgacct gctctgcctg ggctcgaggg
      721 gctacggacc ggtgcggcgg gtgctgctcg gctcggtctc ctccgacgtg atgaaccacg
      781 ccgcctcggc ggttctggtc gtaccccgaa ccgaccaaac caacgactga ccccggcggc
      841 cctgcgggcg gatgccaggg gcctccagcc gtccgctggc aaggagacag gccggaaatg
      901 accgcgatag tacgcgggta cgtgagcgtc gtttccggcc gccgacgaag ccagcggacg
      961 gctggtggtt cctggcgccg cccgctactc gattaaacag ccggactgat tccagaagct
     1021 ggaccagatg ccgaaggcct tcgggaccgc gttggcgatc gcctgcgcgc gcaccttgta
     1081 accctcggag gtgaagtgga tgccgtcatc gatgaaccac tcgtccttga ccttgttggc
     1141 ccagtcgtag acgcgcatgt tcggg
//
LOCUS       DAOQMF010000711         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_111271_length_1164_cov_3.499549, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000711 DAOQMF010000000
VERSION     DAOQMF010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111271_length_1164_cov_3.499549"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /gene="mptA"
                     /locus_tag="PLV77_10475"
     CDS             complement(<1..790)
                     /gene="mptA"
                     /locus_tag="PLV77_10475"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HNC16317.1"
                     /translation="MNPEIALSPNAVDVQSSQPATRVSLSRVGVTGVEKVISIGEDGH
                     YHAELECYVDLSPDQAGAHMSRFEENVSEAIEEVVRSQSMRAEQLAARIAELVRVKQK
                     GLRAEVSINARYAQTMPAPVSGIENQEIYNLLGTAVASPEGTRTVTGVQAQGMTACPC
                     AQGMVADLARTRLRSKGYDEDQVEEIIEEVPIATHNQRGLGTLWIGCTEGGPDLDARD
                     MLRLVESSMSSEIYELMKRPDEMSVVVKAHSQPRFVEDCVREMIR"
     gene            910..>1164
                     /locus_tag="PLV77_10480"
     CDS             910..>1164
                     /locus_tag="PLV77_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HNC16318.1"
                     /translation="MEFETDELKALAASGRDRGYLTFDAVAEAVAELDLGKGDVEELN
                     SWLTEQGIDLISQEESELRGGDGDGRAPERNGSPVLDGTGG"
BASE COUNT          200 a          358 c          389 g          217 t
ORIGIN      
        1 ggcggatcat ctcgcgcacg cagtcctcga cgaagcgggg ctggctgtgg gccttgacca
       61 cgaccgacat ctcgtcgggc cgcttcatca gttcgtagat ctcggagctc attgagctct
      121 cgaccagccg cagcatgtca cgggcatcga gatccgggcc gccttcggtg cagccgatcc
      181 agagggtgcc gaggccgcgc tggttgtgcg tggcgatcgg aacctcctcg atgatctcct
      241 cgacctggtc ctcgtcgtaa cccttgctcc ggagccgggt acgggccagg tcggcgacca
      301 tcccctgggc gcaggggcag gcggtcatgc cctgcgcctg cactccggtc acggtgcggg
      361 tcccttccgg tgaagcgacc gcggtgccga gcaggttgta gatctcctgg ttttcgatcc
      421 cggagaccgg ggccggcatg gtctgcgcgt agcgggcatt gatcgaaacc tcggccctga
      481 ggcccttctg cttgacccgc accagttcgg cgatccgggc ggcaagctgc tcggcccgca
      541 tcgactggct gcggacaacc tcctcgatcg cctcggagac gttctcctcg aaccgggaca
      601 tatgcgcccc ggcctggtcg gggctcaggt ccacgtagca ctcgagttca gcgtggtagt
      661 gaccgtcttc accgatgctt atgaccttct cgacaccggt cactccgacc cgggaaaggc
      721 tcacccgggt ggcgggctgg gaggactgaa cgtccacggc attgggggaa agcgcgattt
      781 cgggattcat tttcctgaga acagcctagt aaccagagtg agcggccggt taagaacact
      841 ccgtataatt ccgcaaccgc gcacgaactc agggtttcca ttagtggaaa ttcgttactg
      901 ggggtagtta tggagttcga aaccgacgaa ttgaaggcac tggccgcttc tggcagggat
      961 cgcggctacc tgaccttcga tgccgtcgcg gaagcggttg cggagctgga tctgggcaag
     1021 ggtgacgtcg aggaactcaa ttcctggctg accgaacagg gcatcgacct gatcagccag
     1081 gaggaatccg agctgcgggg cggcgacggg gacggccggg ctccggagcg aaacggatcg
     1141 cccgtgcttg acggcaccgg cggg
//
LOCUS       DAOQMF010000712         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_111458_length_1163_cov_3.777076, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000712 DAOQMF010000000
VERSION     DAOQMF010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111458_length_1163_cov_3.777076"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1115
                     /locus_tag="PLV77_10485"
     CDS             132..1115
                     /locus_tag="PLV77_10485"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNC16319.1"
                     /translation="MKYLITGGAGFIGSHLADALLARGDEVTVLDNFSTGKHQNLESA
                     LAAGADLIEGDILDRELVEKTLQSVRPQTVFHLAAQGEVQRSIEEPAFDATVNVVGTV
                     NLIEASKEIGLDRFVFASTGGAIYGEGSRIDLPASESGETAPLCQYGLSKRAGEMYLE
                     LYRRMYMFNSVALRFANVYGPRQCPKGEAGAVAIFGELLMAGRNPTVFGDGTQTRDFV
                     YIDDLIDAILTASRTDVEGPINLGSGQETSILELLTSLREAGLSLNGDGPPAGTTFEP
                     EFDRGRPGEVKRIAIDPARAARELGWTPTTALEEGLPLTLRAIGRQSGATV"
BASE COUNT          214 a          413 c          347 g          189 t
ORIGIN      
        1 gccgagcatt ggaccgagag tgcggtcatg ccgaagtatc tggatctcgt gcgccgcgtc
       61 gccgagaaaa aagggcggat cgacatcgtt cgtcgcttgc ctccgtcggc gtcagcgtaa
      121 aggatcgggc catgaaatat ctcatcaccg gcggcgccgg attcatcgga tcgcatctcg
      181 ccgatgctct cctcgcacgc ggtgacgaag tcaccgtgct cgacaatttc tccaccggca
      241 agcaccaaaa cctcgaatcg gcgctggccg cgggagccga tctgatcgag ggcgacatcc
      301 tcgaccggga actcgtggaa aaaactttgc agtcggtccg cccgcagacc gttttccatc
      361 ttgcggccca gggcgaggtc cagcgttcga tcgaggaacc ggccttcgac gcgaccgtga
      421 acgtggtcgg cacggtcaac ctgatcgaag cttcgaagga gattggtctc gaccgcttcg
      481 tcttcgcctc gaccggcggg gcgatctacg gggaaggaag ccggatcgat ttgcccgcca
      541 gcgaatccgg ggaaaccgcc ccgctctgcc agtacgggct ttccaagcgg gccggcgaga
      601 tgtacctcga gctctaccgg cgcatgtaca tgttcaactc ggtcgccctc cgcttcgcca
      661 atgtctacgg ccctcgccag tgtcccaagg gtgaagccgg cgcggtcgcg atcttcggcg
      721 agctcctgat ggccggccgg aacccgaccg tcttcggcga cggcacccag acccgcgact
      781 tcgtctacat cgacgacctg atcgacgcca tcctcaccgc ctcccgcacc gacgtcgaag
      841 gcccgatcaa cctcggctcc ggccaggaaa cctcgatcct cgagctcctg accagcctcc
      901 gcgaagccgg cctctcactt aatggagacg gcccgcccgc cggaaccacc ttcgagcccg
      961 agttcgaccg cggccgcccg ggcgaggtca aacgaatcgc catcgatccc gcccgcgccg
     1021 caagagaact gggctggacg ccgaccaccg cgctcgaaga gggcctcccc ctgaccctcc
     1081 gcgcgatcgg ccgccagtcg ggcgcaacag tctgaaggcc gatccagtct ttccgggggc
     1141 gatcagccac cgaatcggtg gcc
//
LOCUS       DAOQMF010000713         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_111954_length_1161_cov_1.867089, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000713 DAOQMF010000000
VERSION     DAOQMF010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111954_length_1161_cov_1.867089"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLV77_10490"
     CDS             <1..229
                     /locus_tag="PLV77_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNC16320.1"
                     /translation="SYREWMLEVINYGVERGEFSTDDPGASADVAMGLLDGLGIRALI
                     EDPDMDVERVRVLAATRIARELGIEPRALTE"
     gene            266..>1161
                     /locus_tag="PLV77_10495"
     CDS             266..>1161
                     /locus_tag="PLV77_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC16321.1"
                     /translation="MSELELNRRQLLLAGAGLAAAAWGVSGCTVERPIDKSAAGDVVE
                     PKIDGDLLIFNWAQYMDPSIKKSFSDRYGVAVNEVNFDNLEAMVTKLRAGGSYDLIFP
                     STEYVQRLRNEQLIRPFDRAKLKSAATLTPYFDNPWYDPNSEFSVPYTYYTTGIAWNT
                     DEVDTMTGSWNDLSNEQALGRIFMLDDFQEGIGQANLLNGFYLNTEKSDQLETSKGTL
                     LDQKEGLRGFSTNSVQNLVSGSAAVTQAWNGDVVNARNQVDDPSVFAYETCTEGVPVG
                     SDLMCIPITSKSPGTAMVFMDW"
BASE COUNT          233 a          364 c          384 g          180 t
ORIGIN      
        1 cagctaccgg gaatggatgc tggaagtcat caactacggg gtcgagcgcg gggagttctc
       61 tacggacgat cccggggcca gcgccgacgt tgcgatgggc ctgctggacg ggctgggaat
      121 cagggccctg atcgaagacc cggacatgga cgtcgaaagg gtccgggtgc tggccgcgac
      181 gcgaatcgcc agggaactcg gaatcgagcc gagggcgctg accgaatgat cgccgcccgc
      241 accgacatcg ggaaggtgcg ggccggtgag tgaactagag ctgaaccgac gccagctgct
      301 gctggccggg gcggggctgg ctgctgccgc ctggggggtc agcggctgca cggtcgagcg
      361 gccgatcgac aagtcggcgg ccggggacgt ggtcgagccg aagatcgacg gcgacctgct
      421 gatcttcaac tgggcccagt acatggaccc ctcgatcaag aagtccttct ccgacaggta
      481 cggggtcgcc gtgaacgagg tcaacttcga caacctcgag gcgatggtga ccaagctcag
      541 ggcgggaggc tcctacgacc tgatcttccc ctcgaccgag tacgtccagc ggctcaggaa
      601 cgagcaactg atccggccct tcgaccgcgc gaagctgaag agcgcggcga cgctgacgcc
      661 ctacttcgac aacccctggt acgaccccaa ctccgagttt tcggtgccct acacgtatta
      721 cacgaccggg atcgcctgga acaccgatga ggtcgacacg atgaccggct cctggaacga
      781 cctttcaaac gagcaggccc tgggccggat cttcatgctc gacgacttcc aggaggggat
      841 cggccaggcc aacctgctga acggcttcta cctgaacacc gagaagtccg accagctcga
      901 aacctcgaag ggcacgctgc tcgaccagaa ggagggtctg cgcggctttt caaccaactc
      961 ggtccagaac ctggtttccg ggtcggcggc cgtgacccag gcctggaacg gggacgtggt
     1021 caacgccaga aaccaggttg acgacccctc ggtcttcgcc tacgaaacct gcacggaagg
     1081 ggtcccggtc ggctcggacc tgatgtgcat cccgatcacc tcgaagagcc ccggcacggc
     1141 gatggtcttc atggactgga t
//
LOCUS       DAOQMF010000714         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_112258_length_1159_cov_2.836957, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000714 DAOQMF010000000
VERSION     DAOQMF010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112258_length_1159_cov_2.836957"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLV77_10500"
     CDS             complement(<1..323)
                     /locus_tag="PLV77_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNC16322.1"
                     /translation="MKICVLVKDVPDAAVQKRIDPGTMRLDRSGENTLNPFDTHAIEA
                     AMQLREGGELQVDEIVAVTMGTDTAARALQKAVALGADRSVHLSDDALAGSDVCATGY
                     ALAKVL"
     gene            complement(548..>1159)
                     /locus_tag="PLV77_10505"
     CDS             complement(548..>1159)
                     /locus_tag="PLV77_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16323.1"
                     /translation="AQSILREGSTFQWSTVTLLGLVVYVYSVEVERRRWDIVLAGLAF
                     SMMDLFNEMVNSAIFHLTDRAPLWTVTGDTSYLIFIGLTIEILLLFAVSGVAFVKTLP
                     PDRKAKILGIPNRIVFVTVFSLFCVAVEIFLHSTGYFHWEYWWWNVPFVPLIVIFGYA
                     TFFAVAAWVYDMGSNTRKQVRVVGTMAAINLVLGLALGLAGWL"
BASE COUNT          233 a          340 c          387 g          199 t
ORIGIN      
        1 agcaccttcg ccagggcgta gccggtggcg cagacgtcgg agccggcgag ggcgtcgtcg
       61 gacaggtgga ccgaacggtc ggcgccgagg gcgacggcct tctgcagggc gcgggcagcg
      121 gtatcggtgc ccatcgtcac ggcgacgatt tcgtctacct ggagttcgcc gccttcgcga
      181 agctgcatcg cagcctcgat ggcgtgggtg tcaaaggggt tgagagtgtt ctcgccgctg
      241 cgatcgagcc gcatggtgcc gggatcaatg cgcttttgta ccgcggcgtc tggcacatct
      301 ttgaccagga cgcaaatctt caactgaagc ctccttaggg ggtcttacga ggggctctgc
      361 cgaattcaag ggcggagagc cggagatgct gttttgcgac gcgggattct atcgggattc
      421 gccaaaagtt gactgacgag tcaatcagag ggcttctgca gtcgggcggg tcttcacccg
      481 ccctccggcc tggaacccgc ccctcccact cgaagtcaat gtttgtcggg aggggatcag
      541 ggagtcccta caaccaaccg gccagtccca atgccaggcc tagaaccaga tttatggcgg
      601 ccatggtgcc caccaccctg acctgtttcc gggtgttcga tcccatgtca tagacccagg
      661 cggcgaccgc gaagaaggtg gcgtagccga agatcacgat cagcgggacg aagggcacgt
      721 tccaccacca gtactcccag tggaagtagc cggtgctgtg caggaagatc tcgaccgcca
      781 cgcagaagag cgagaagacg gtcacgaaaa cgatccggtt cgggatcccg aggatcttcg
      841 ccttccggtc gggcggcagg gtcttgacga aggcaacccc ggaaaccgcg aagagcagca
      901 ggatctcgat cgtcaggccg atgaagatca ggtagctggt gtcaccggtc acggtccaga
      961 gcggcgcccg gtcggtcagg tggaagatcg ccgagttgac catctcgttg aagagatcca
     1021 tcatcgagaa ggcgagcccg gccagcacga tgtcccagcg ccggcgttcg acctcgaccg
     1081 aatacacgta gacgaccagg ccgagcaggg tgaccgtgga ccactggaag gtggatcctt
     1141 cgcgcaggat ggactgggc
//
LOCUS       DAOQMF010000715         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_112443_length_1158_cov_2.917498, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000715 DAOQMF010000000
VERSION     DAOQMF010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112443_length_1158_cov_2.917498"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1158
                     /locus_tag="PLV77_10510"
     CDS             <1..>1158
                     /locus_tag="PLV77_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16324.1"
                     /translation="VTHPRELIRRTFGNPDEGTAGRKARLAGLIGVPVAGAFLIGVIL
                     GVIGQSLSAGATFILLAIPVAIGGGILTGRAVLGGAMQLRGNQKAGWITEKLEPLNVA
                     ADPGQPLRVEIIHPAVDLKHFFGGFIAVFNLARRLGEGGHRVRIVTLEPLDLPDDWRR
                     QVAAYQGIGDSVGDLEVIDGSDRAVPVAMNPDDRLIATHWTAAHIASAAAAELDQESF
                     TYLIQEYEPLIFPMGSAAALADQSYELDHNAIFSTGFLRDYFRDQGIGVYRPEGPGEA
                     GAAVFDNAITPAATVTTGELQGKDRRRLLFYARPEAHAERNLFELGIMALDRAILAGH
                     FRGWDLAGIGNVDGTGELTLPQSKARLRMLPRVAQREYADTLRQFDVGLALM"
BASE COUNT          180 a          396 c          399 g          183 t
ORIGIN      
        1 gtgacgcacc cccgggagct gatccgacgg acttttggca accccgatga aggcaccgcc
       61 ggacgcaagg cgaggctggc cgggctgatc ggcgtgccgg ttgccggggc ctttctgatc
      121 ggcgtgatcc tcggggtgat tggccagagc ctctcggccg gcgcgacctt catcctcctg
      181 gcgattccgg tggcgatcgg cggcggcatc ctgaccggcc gggccgtgct ggggggagcg
      241 atgcagctgc gcggcaacca gaaggccggc tggatcaccg agaaactgga accgctgaac
      301 gtcgcggccg accccggcca gcctttgcgg gtcgagatca tccaccccgc cgtcgacctc
      361 aaacatttct tcggtggctt catcgcggtc ttcaacctgg cccggcggct gggcgagggc
      421 ggccaccggg tccggatcgt caccctggag ccactcgacc tgcccgatga ctggcgccgg
      481 caggtcgccg cctatcaggg gatcggtgac tcggtgggtg acctggaagt gatcgatggc
      541 agcgatcggg ccgtcccggt cgcgatgaat ccggatgacc gcctgatcgc cacgcactgg
      601 actgccgccc acatcgcctc ggctgctgcc gcggaactcg atcaggagtc cttcacttac
      661 ctgatccagg aatacgagcc gctgatcttt ccgatgggca gcgccgccgc cctcgccgat
      721 cagagctacg aactagacca caacgcgatc ttctcgaccg ggttcctgcg ggactacttc
      781 cgcgaccagg gaattggcgt gtaccggccg gaaggaccgg gggaagcggg cgccgcggtc
      841 ttcgacaacg cgatcacccc ggcggccacg gtcaccaccg gcgagcttca gggcaaggac
      901 cggcgccggc tcctgttcta cgcgcgcccc gaggcccacg ccgaacgcaa cctcttcgag
      961 ctggggatca tggccctcga ccgggcgatt ctcgctggcc atttccgggg ctgggacctg
     1021 gccgggatcg ggaacgtgga cgggaccggg gagctgaccc tgccgcagtc gaaggcccgg
     1081 ctgcggatgt tgccgcgggt cgcccagcgg gagtacgcgg acacgctacg ccagttcgac
     1141 gtcggcctgg ccctgatg
//
LOCUS       DAOQMF010000716         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_112960_length_1155_cov_5.162727, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000716 DAOQMF010000000
VERSION     DAOQMF010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112960_length_1155_cov_5.162727"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLV77_10515"
     CDS             complement(<1..246)
                     /locus_tag="PLV77_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC16325.1"
                     /translation="MAVVGTLVVILAIFALIVLFSAIKILREYERGVIFRLGRLINQK
                     GPGLILLIPFIDRMVKIDLRPVTLNIPPQEVITRDNVP"
     gene            complement(249..863)
                     /locus_tag="PLV77_10520"
     CDS             complement(249..863)
                     /locus_tag="PLV77_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNC16326.1"
                     /translation="MEISDPTLAYLLIILGLTGIGIEAITPGGFVPAILGIIALVFGV
                     IGVVDIGPTAIGLGLLLLAIALFISAIAFHLYRPLSIAGVISLVASGIWMFDRETDPT
                     SIIAVTIGAVALGLFMLFVIERASETRAAPVRYGPEDLVGMPGEVRVPLRPDGQVFVD
                     GALWQARLADSTARLGVGEKVTVTEVSGLTLLVAPASETNQGEI"
     gene            complement(907..>1155)
                     /locus_tag="PLV77_10525"
     CDS             complement(907..>1155)
                     /locus_tag="PLV77_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16327.1"
                     /translation="GVNSDDNSDAAKTFLSTHPVPYPSYEDPDKDIAQSLGATHGLPG
                     TAFFNADGELTYTRSGPYSSDQELEADVQKYAIEGQSG"
BASE COUNT          211 a          351 c          402 g          191 t
ORIGIN      
        1 gggcacgttg tcgcgggtga tcacttcctg cggcgggatg ttcagggtca cgggtcgcag
       61 gtcgatcttg accatgcggt cgatgaaggg gatcagaagg atcagcccgg gacccttctg
      121 gttgatcagc cggccaagcc ggaagatcac gccgcgctcg tattcgcgca ggatcttgat
      181 cgccgaaaac aggacgatca gggcgaagat cgcgaggatc acgaccaggg ttccgacgac
      241 tgccatctct agatctctcc ttggttggtt tctgaggcgg gggcgaccag aagggtcagg
      301 ccgctgacct cggtgacggt gaccttctcg ccgacgccga gccgggctgt ggagtcggcg
      361 aggcgggcct gccagagtgc gccgtcaacg aagacctggc catccggccg gagcggcacc
      421 cggacttcgc ccggcatccc gaccaggtcc tcggggccgt aacggaccgg ggctgccctg
      481 gtttcactgg cccgctcgat cacgaaaagc atgaagagtc cgagcgcaac cgcgccgatc
      541 gtcaccgcga tgatcgaggt cgggtcggtc tcacggtcga acatccagat gcccgaggcg
      601 accagtgaaa tcactccggc gatcgagagg ggccggtaga ggtggaaggc gatcgcggag
      661 atgaacaggg cgatcgccag cagcagcagg ccgagcccga tcgccgtcgg cccgatgtcg
      721 accactccga tcaccccgaa gaccagagcg atgatgccga gaatcgccgg cacgaaaccg
      781 ccgggcgtga tcgcctcgat cccgatcccg gtcaggccga gaatgatcag caggtaggcg
      841 agtgtcggat cactgatctc cacggacccg agcgtatcgg tcctgcccgg tgggcagtaa
      901 gcgggctcag ccggactgac cctcgatcgc gtacttctgg acgtctgctt ccagctcctg
      961 gtcgctggag taggggccgg aacgggtgta ggtcagttcc ccgtctgcgt tgaagaacgc
     1021 cgtcccgggc aggccatggg tggccccgag ggactgggcg atgtccttgt ccgggtcctc
     1081 gtagctgggg taggggaccg gatgggtcga gaggaaagtc ttcgccgcgt ccgagttgtc
     1141 atccgagttc acgcc
//
LOCUS       DAOQMF010000717         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_113627_length_1152_cov_3.216955, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000717 DAOQMF010000000
VERSION     DAOQMF010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113627_length_1152_cov_3.216955"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLV77_10530"
     CDS             <1..647
                     /locus_tag="PLV77_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNC16328.1"
                     /translation="AARGEQLARIWMHNGMIETGSEKMSKSVGNIFQLSEALDAYGAE
                     TVVAYLISGHYRQPLAFGEGPLEQAAAGNERLRNFLRENPPAGAGEGVPGDEAAAAGS
                     RFRDALADDFNTPRAMREAFDLVRAVNQRPDDPALAGAGTVLAGMLELVGLESLATGG
                     EEGPGDEALELLEARNAARADKDWARADEIRDSLAELGWEVRDGAEGARLVPRS"
     gene            647..>1152
                     /locus_tag="PLV77_10535"
     CDS             647..>1152
                     /locus_tag="PLV77_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC16329.1"
                     /translation="MSDPEIIYGRNPVEEAKRGRRKVLRVWEAPETPETRLVELCGST
                     DHQGIVAEVEPFPYLSGSDLLAGEDALVVVLDQIQDPRNLGAICRSAEAAGATGVVIP
                     DRRAASVTAAAAKASAGAVEHLKIARVRNISDWIIEAKDAGFWIWGADGRADQAPWQV
                     DLTGKTALV"
BASE COUNT          188 a          386 c          422 g          156 t
ORIGIN      
        1 aggcggcccg gggcgagcag ctggcccgga tctggatgca caacgggatg atcgaaaccg
       61 gctcggaaaa gatgtcgaag tcggtcggga acatcttcca gctttccgag gcgctcgacg
      121 cctacggagc cgaaaccgtg gtcgcctacc tgatttccgg tcactaccgc cagccactcg
      181 ccttcggcga gggcccgctc gaacaggcgg ctgccggcaa cgaacgcctg cgcaacttcc
      241 tgcgcgaaaa tccgccggca ggggcggggg agggcgtgcc cggcgacgag gccgcggcgg
      301 ccggttcccg gttccgggac gcgctggccg atgatttcaa cactccccgg gcgatgcgcg
      361 aggccttcga cctggttcgc gccgtcaatc agcggcccga cgaccccgcc ctggccggtg
      421 ccgggaccgt tctggccggg atgctggagc tggtcggcct ggaatccctc gccacgggcg
      481 gggaggaggg acccggggac gaggcgctcg agctgctcga ggcaagaaac gcggccaggg
      541 ccgacaagga ctgggcccgc gccgacgaga tccgggacag tctggccgaa ctcggctggg
      601 aggtccgtga cggggccgaa ggcgcccggc tcgtgccccg cagctgatgt ccgacccgga
      661 gatcatctat ggccgcaatc ccgtcgagga ggccaagcgg ggccggcgaa aggtgctccg
      721 ggtctgggaa gcccccgaaa cgccggagac caggctggtc gagctttgcg gttcgaccga
      781 ccatcagggg atcgtggccg aggtcgagcc tttcccctac ctgagcgggt ccgacctgct
      841 ggccggggag gacgcactgg tcgtggtcct cgaccagatc caggaccccc gcaacctcgg
      901 tgcgatctgc cgttcggccg aagccgcggg ggcgaccggg gtggtgatcc ccgatcgacg
      961 ggccgcttcg gtcaccgccg cggcggccaa ggcttcggcc ggcgcggtcg agcatctgaa
     1021 gatcgcccgg gtccgcaaca tcagtgactg gatcatcgaa gccaaggatg cgggcttctg
     1081 gatctgggga gccgacggac gcgcagacca ggccccctgg caggtggacc tgaccggcaa
     1141 gaccgccctc gt
//
LOCUS       DAOQMF010000718         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_113901_length_1151_cov_2.318431, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000718 DAOQMF010000000
VERSION     DAOQMF010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113901_length_1151_cov_2.318431"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLV77_10540"
     CDS             <1..374
                     /locus_tag="PLV77_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC16330.1"
                     /translation="GVELEVTPQGKRMRQMSLLSGGEKAMTALAFVFAVFLARPCPFY
                     ILDEVEAALDDANINRFLELVRRFSDRTQFVIVTHQKLTMDAADVLYGVSMGGDGVTK
                     VISRRLPKGVTALEGTQLEAA"
     gene            420..>1151
                     /locus_tag="PLV77_10545"
     CDS             420..>1151
                     /locus_tag="PLV77_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC16331.1"
                     /translation="MSTTWNDILDLGDRAVPDTAPPPAAEEEAPKKMGAFRRLRESLS
                     KSRRALTEEIGSSLFDRIDETWERLEEALILADAGATTTAQVVEKLEYEVDSGAVPPD
                     GAAIKERLIGILTEIASTNRAPINLSQDPAVVLMTGVNGTGKTTSIGKIAWHLRNEFG
                     LSVLLGAADTYRAAAAEQLATWAERAGADLVRSNEGADPGSVAFDAINAARSRGSDVV
                     IIDTAGRLHTQSNLMEELAKVRRVIE"
BASE COUNT          208 a          402 c          371 g          170 t
ORIGIN      
        1 tcggggtcga actcgaagtc acaccgcagg gcaagcgcat gcgccagatg agcctgcttt
       61 ccggcggcga gaaggcgatg accgcgctgg ccttcgtctt cgcggtcttc ctcgcccggc
      121 cctgcccgtt ctacatcctc gacgaggtcg aggccgccct cgatgacgcc aacatcaacc
      181 gtttcctcga actggtccgc cgcttctcgg atcgcacaca gttcgtgatc gtgactcacc
      241 agaagctgac catggacgcg gccgacgtgc tctacggggt cagcatgggt ggcgacggcg
      301 tgaccaaggt gatctcccgt cgcctgccga agggggttac cgccctcgaa ggcacgcagc
      361 tggaagccgc gtaaccgctt ctctgacacc ggcaacccga ttccggcaag atccgaaccg
      421 tgagcacgac ctggaacgac atcctcgacc tcggtgaccg cgccgtcccc gacaccgccc
      481 cgccacccgc cgccgaggag gaagcgccga agaagatggg cgcgttccga cggcttcgcg
      541 aaagcctctc gaagagccgc cgggcgctga ccgaggaaat cggttccagc ctcttcgacc
      601 ggatcgacga gacctgggag cgcctcgagg aagcgctgat cctcgccgac gccggagcga
      661 ccacgaccgc ccaggtggtc gagaagctcg aatacgaggt cgattccgga gcggtgccgc
      721 cggatggcgc ggcgatcaag gagcgcctga tcggcatcct gaccgagatc gcctcgacca
      781 accgggcgcc aatcaacctc agccaggatc ccgccgtggt cctgatgacc ggggtcaacg
      841 gcaccggcaa gaccacctcg atcggcaaga tcgcctggca cctcaggaac gagttcgggc
      901 tgtccgtcct gcttggcgcc gccgacacct accgggcggc cgcggccgag cagcttgcga
      961 cctgggccga gcgggccggg gcggacctgg ttcgctcgaa cgaaggcgcc gaccccggct
     1021 cggttgcctt cgacgcgatc aatgccgcca ggtcccgcgg ctccgatgtt gtgatcatcg
     1081 atacggccgg ccggcttcac acccagtcga acctgatgga ggagctggcg aaggtcaggc
     1141 gcgtgatcga g
//
LOCUS       DAOQMF010000719         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_114042_length_1150_cov_3.152511, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000719 DAOQMF010000000
VERSION     DAOQMF010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114042_length_1150_cov_3.152511"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..>1150)
                     /locus_tag="PLV77_10550"
     CDS             complement(103..>1150)
                     /locus_tag="PLV77_10550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNC16332.1"
                     /translation="GSVGSATLLYGLAMIYGASGATGFQAISDGLQAGDGMLSDSLTL
                     VGVGLVAVGLAFKVSIAPFHQWTPDVYQGAPTPVTAFMAVATKAAAFAVFIRFFLVAL
                     GPLADQWDVMLAALAALSIVVGNVGALAQTSLKRMLGYSGIAQAGYMLAGIVTATQAG
                     VDALVFYLAAYVAMNVGAFAVVVARERQTGFSDDISTLRGLGREKPLLAWPLTISMLA
                     LAGLPPTVGFIGKLYLIEALVDDDWTWLAVMIAIGTMISLGYYLRVVATVWMSPAEES
                     EAAAASIDTAALPPQIAGAQADPFTDSRADRRWYLVAPALIAMAAALFFGVIPQPLVD
                     FAQHAGNALSVWIS"
BASE COUNT          229 a          352 c          427 g          142 t
ORIGIN      
        1 accgacagcg ttgaagttat ccgcacatgg aacgaataac ttcaacccag atgcggggcg
       61 ggttgagacc tggagggcga gcgaagactc gcccgactgc ggttaggaga tccagacgct
      121 tagggcgttg ccggcgtgct gggcgaagtc gaccaggggc tgggggatta cgccgaagaa
      181 gagggcggcg gccatggcga tcagcgccgg ggcgacgagg taccagcggc gatcggcccg
      241 ggagtcggtg aaggggtcgg cctgggcgcc ggcgatctgg ggcggcaggg cggcggtgtc
      301 gatcgaagca gcggcggctt cggactcttc cgccgggctc atccagacgg tggcgaccac
      361 ccgcaggtag tagccgagcg agatcatcgt gccgatcgcg atcatcaccg ccagccaggt
      421 ccagtcgtca tcgaccaggg cctcgatcag gtacagcttg ccaatgaagc cgacggtcgg
      481 cggcaggccg gcgagggcca gcatcgagat cgtcaaaggc caggcgagca ggggcttttc
      541 gcgaccgaga ccgcgcaggg tcgagatgtc atcggagaag ccggtctgac gctcgcgggc
      601 cacgaccacc gcgaaggcac cgacgttcat cgcgacatag gcggcgaggt agaaaaccag
      661 ggcgtccaca ccggcctggg tcgcggtgac gatcccggcc agcatgtacc cggcctgggc
      721 gatgccggaa tagccgagca tgcgcttcag cgaagtctgg gcgagggcgc cgacgttgcc
      781 gaccacgatc gagagcgcgg ccagggcggc cagcatcacg tcccactgat cggccagcgg
      841 cccgagtgcg accaggaaga agcggatgaa gacggcgaag gcggcggcct tggtcgcaac
      901 cgccatgaag gccgtaaccg gggtcggggc gccctggtag acatccggcg tccactgatg
      961 gaagggcgcg atcgaaacct tgaaggcgag gccgaccgcg accaggccga ctccgaccag
     1021 ggtcagagag tccgaaagca tgccgtcgcc ggcctgcagc ccgtcactga tcgcctggaa
     1081 gccggtcgcg ccggaggcgc cgtagatcat cgccaggccg taaagcaggg tggccgaacc
     1141 gaccgaaccg
//
LOCUS       DAOQMF010000720         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_114056_length_1150_cov_2.984475, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000720 DAOQMF010000000
VERSION     DAOQMF010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114056_length_1150_cov_2.984475"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1150
                     /locus_tag="PLV77_10555"
     CDS             <1..>1150
                     /locus_tag="PLV77_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNC16333.1"
                     /translation="GWVNIMADAPGEPLPPLSPATGQPAGPDDLSAIFPMSLIEQEMS
                     PEETIEIPEEVRETYKLWRPTPLIRATRLEKALDTPAHIYYKYEGTSPAGSHKLNSAV
                     AQAYYNREAGTRKLVTETGAGQWGSALSLACQWFGIECEVFMVGVSYDQKPYRRAMIE
                     TWGASVHRSPSDLTEAGRSQAAHESGSLGIAISEAVEVAAGSEDANYALGSVLNHVCL
                     HQTVIGQEAIAAMEMAGEYPDVVVGCVGGGSNFAGLINPFLRENIRNGKTTRFLGAEP
                     AACPTLTRGKYAYDFGDTVGMTPLMPMYTLGHDFVPAPVHAGGLRYHGDAPSLCAFVH
                     EGFVEATAVPQGAAFEAALLFARTEGIIPGPEPAHAIRAVIEEAEAAK"
BASE COUNT          203 a          404 c          363 g          180 t
ORIGIN      
        1 ccggctgggt caacatcatg gccgacgctc cgggcgagcc cttgccgccg ctcagcccgg
       61 ccaccggcca gccggccggg cccgatgacc tctcggcgat cttcccgatg agcctgatcg
      121 aacaggagat gtcaccggaa gaaacgatcg agatcccgga agaggtgagg gagacttaca
      181 agctctggcg gccgaccccg ctgatccggg cgacccgcct cgagaaggct ctcgacaccc
      241 ccgctcacat ctattacaag tacgaaggca cttccccggc cggctcccac aagctgaact
      301 cggccgtcgc gcaggcctat tacaaccggg aagccggcac ccggaaactg gttaccgaaa
      361 ccggggcggg gcagtggggc tcggccctct cgctcgcctg ccagtggttc gggatcgaat
      421 gcgaagtgtt catggtcggg gtttcctacg accagaagcc ctatcgccgg gcgatgatcg
      481 aaacctgggg tgcctcggtt caccgcagcc cctcggacct gaccgaagcg ggccgttccc
      541 aggcagctca cgagagcggc agcctcggca tcgcgatctc ggaagcggtc gaggtcgcgg
      601 ccggctccga ggacgccaac tatgccctgg gttccgtcct caatcacgtc tgcctccacc
      661 agaccgtgat cggccaggaa gcgatcgccg cgatggagat ggccggcgaa tacccggatg
      721 tggtggtcgg ctgcgtcggt ggcggctcga acttcgccgg cctgatcaac cccttcctgc
      781 gcgagaacat ccgaaacggc aagacgaccc gcttcctcgg cgcggaaccg gccgcctgcc
      841 cgaccctgac ccggggcaag tacgcctatg acttcggtga cacggtaggc atgactccgc
      901 tgatgccgat gtacacgctc ggtcatgact tcgtgcccgc acccgttcac gcgggaggcc
      961 tcaggtatca cggtgatgcc cccagcctct gcgccttcgt tcacgagggt ttcgtcgagg
     1021 cgaccgccgt tccccagggt gcggccttcg aggcggcgct gctcttcgcc cgcaccgaag
     1081 ggatcattcc cggcccggaa ccggcccatg cgatccgcgc cgtcatcgag gaagccgaag
     1141 cggcgaagca
//
LOCUS       DAOQMF010000721         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_114317_length_1149_cov_2.156307, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000721 DAOQMF010000000
VERSION     DAOQMF010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114317_length_1149_cov_2.156307"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLV77_10560"
     CDS             complement(<1..414)
                     /locus_tag="PLV77_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNC16334.1"
                     /translation="MTDSEETGEAITAEGLARLKEELEELEGPARDRIAERLKVAREL
                     GDLKENAEYHIAKEDQAHLETKIQRLREREKNAVVAEVEDSGDSFAFGRTAEVTDDSG
                     RSHKWTLVGSTEANLAEGRLSAESPVGQALRDRKVG"
     gene            complement(499..>1149)
                     /locus_tag="PLV77_10565"
     CDS             complement(499..>1149)
                     /locus_tag="PLV77_10565"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNC16335.1"
                     /translation="IAGAMDTIYTASPLVGTTATLAAGVEQFEPSSDLKARVMAAVDA
                     DLAEHPVPSPDPIEVDSAPRPRPERRPVARWLSGILRPAVLGAVAAALFVGVALGVVL
                     NGGDDTSPTGPSRQVLTGSSTIGADAVMVASDGTGTLKMTNLKRPDDDQVYQAWIQRG
                     QEIEPTESLFVPNKDGSAVASIPDISGVTAVMVSAEPRGGSPQPTTAPVITVSMPS"
BASE COUNT          166 a          376 c          394 g          213 t
ORIGIN      
        1 gccgaccttg cggtcacgga gcgcctgacc gaccggtgat tcggcgctga gcctgccttc
       61 ggccaggttg gcttcggttg agccaaccag cgtccatttg tggcttcggc cggaatcgtc
      121 ggtcacctcg gcggtccggc cgaaggcgaa ggaatcaccc gaatcttcca cctcggcaac
      181 taccgcgttc ttctcacgct cgcgaagccg ctggatcttg gtttcgaggt gcgcctgatc
      241 ctccttggcg atgtggtact cggcgttctc cttgaggtcg ccgagctccc gggcgacctt
      301 gagccgctcg gcgatccggt cccgggccgg gccttcgagt tcttcgagtt cctccttgag
      361 ccgggccagt ccttcggcgg tgatcgcctc gccagtctcc tctgaatcgg tcatggaagc
      421 agcctacttg ggccgcggtc cggcccgggc accggagaga gaggagagtg cccgggccgg
      481 gcgccgctgg agggaaaatc aactgggcat cgagacggtg atcaccggtg cagtggtcgg
      541 ctgggggctg ccacccctgg gttcggcgct caccatcacc gcggtcactc cgctgatgtc
      601 gggaatgctc gccacggctg acccgtcctt gttgggaacg aagagggatt cggtgggttc
      661 gatttcctgg ccgcgctgga tccaggcctg gtagacctga tcgtcgtcgg gtcgtttgag
      721 attcgtcatc ttcagcgtgc cggtgccgtc ggaggcgacc atcaccgcat ccgctccgat
      781 ggtcgaggaa ccggtcaaga cctgtcggga tgggccggtc ggcgaagtat cgtcgccgcc
      841 attgagtacg actccgagcg cgacccccac gaacagggcc gcggcgaccg ctccgagaac
      901 cgccggccgg aggatcccgc tcagccacct tgctacgggg cggcgctccg gtctcggccg
      961 gggagcggaa tccacttcga tcggatcggg ggacggcacg ggatgctctg ccaggtcggc
     1021 gtcgaccgcg gccatcaccc gcgccttcag gtcggaggac ggctcgaact gctcgacccc
     1081 ggctgcgagc gtggcagtgg tgccgacgag cggcgaggcc gtgtaaattg tgtccatcgc
     1141 acccgcgat
//
LOCUS       DAOQMF010000722         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_114482_length_1148_cov_2.449222, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000722 DAOQMF010000000
VERSION     DAOQMF010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114482_length_1148_cov_2.449222"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLV77_10570"
     CDS             <1..714
                     /locus_tag="PLV77_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNC16336.1"
                     /translation="LSYGHRLEPYIADTARLCWNELDAGNKVIFEGAQAALLDLDHGT
                     YPFVTSSNPIAGAACVGAGVGPTDIEEVWGIVKAYSTRVGSGPFPTELFDEVGEYMSD
                     KGHEFGTTTGRRRRCGWLDLVALKYAVRLNRLSHLAMTKLDVLTGLDTIRVAVRYRSK
                     EGALLEDFPYHQSILHNATPEYVDLPGFSEDITGCRTEEDLPPAAREYISFISDYVGV
                     PINLVGVGPARDEVVWIQS"
BASE COUNT          199 a          367 c          368 g          214 t
ORIGIN      
        1 ctgagctacg gccaccggct ggagccctac atcgccgaca ccgcccggct ctgctggaac
       61 gagctcgacg ccggcaacaa ggtgatcttc gagggcgccc aggcggccct gctcgacctc
      121 gatcacggca cctatccctt cgtcacttcc tcgaatccga tcgccggagc cgcctgcgtc
      181 ggggccgggg tcggaccgac cgacatcgaa gaggtctggg gcatcgtcaa ggcctactcg
      241 acccgggtcg gttcggggcc cttcccgacc gaactcttcg acgaagtcgg cgagtacatg
      301 tcggacaagg ggcatgaatt cggcaccact accggccggc gtcgccgctg cggctggctt
      361 gacctggtcg ccctcaagta cgcggtgcgg ctgaaccggc tttcacacct ggcgatgacc
      421 aagctcgacg tcctgaccgg cctggacacg atcagggtcg cggtgcgcta ccgctcgaag
      481 gaaggtgcgc tgctcgagga ctttccctac caccagagca tcctccacaa cgcgactccg
      541 gagtacgttg acctgcccgg attcagcgag gacatcaccg gctgccggac cgaggaggat
      601 ctgccgccgg ccgcccggga atacatcagc ttcatcagcg actacgtcgg ggtgccgatc
      661 aacctggtcg gggtcggacc ggcccgcgac gaggtggtct ggatccagtc ataagcagtc
      721 gggccggcac ggactgaccg cagtcgggcg ggtctttgcc cgccctccgg gatcaacccg
      781 cccctcccac ttgaagtaac tgatggttgg gaggggctca ggtggtgctg gttcggggac
      841 tcaggtcggg ctggttcggg ggctcgggtg gtgctggttc gagcgggggg tgggacaggt
      901 ttcggaaacg cccctgaaag actttttgcc gcgggacaca taaacgcgtg tgtttggtgt
      961 actatgtcgt atcagcagtt agatcactgg acgtgataac tgcaaaacat cgttgactgg
     1021 ggtccctccc taccctcagc agcgcttagt cggtgaagtc gggttggccc actctccctt
     1081 gggtcagccc gaccgccgac attttttatg cctcgaatcc cccttgaaca agggaattgc
     1141 gctccagg
//
LOCUS       DAOQMF010000723         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_114986_length_1145_cov_4.063303, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000723 DAOQMF010000000
VERSION     DAOQMF010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114986_length_1145_cov_4.063303"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1145
                     /locus_tag="PLV77_10575"
     CDS             <1..>1145
                     /locus_tag="PLV77_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16337.1"
                     /translation="LYYNGPGLVGRKLVSAIRDGDRAEAWFEIRYNSNNGNSLNSGLA
                     KRRYREAEMFGLYNPGEVTADDAEKVYRMFTLHRREIEAYERKYGIDFEGDLGTARVQ
                     AVGGSYVPLIAANLQWSLTGASAVDVIHEALTPARDRLFADLMAANPVGMAGLAASDF
                     SPTSIFLDPGRIDVSAAVDRNHRGVLDARYDVELFERYDDDILIGGAGDDWLLGGKGD
                     DLLIGGAGNDTYIYREGDGDDRIIDSDKSGRIIVVSPDNRYEYAAGTFLKDPGGSELW
                     INLDDHIRITHNSPWKLVLADGSEIQLGEDFADGDYGINLEDYSTAIVPALTVAGDLA
                     PVDRDPATPGIQVGYDACGNLIVGGAAEPGRADRLYGSGLSDQVTAG"
BASE COUNT          220 a          378 c          362 g          185 t
ORIGIN      
        1 cctctattac aacggacccg gattggtggg caggaaactc gtatcggcca ttcgggacgg
       61 ggaccgtgcc gaagcctggt tcgaaatccg ctacaactct aacaacggca atagtctgaa
      121 ctcgggactc gcgaagcgcc gctatcgcga ggctgagatg ttcggcctgt acaacccggg
      181 cgaggtcacc gccgacgacg cggaaaaggt gtatcggatg ttcaccttgc atcggcgcga
      241 gatcgaagcc tacgagagaa aatacggaat cgacttcgaa ggggacctgg gaacggctcg
      301 ggtccaagcc gtgggcggca gctacgtccc gctgatcgcg gcgaatctgc aatggagcct
      361 gaccggcgcc agtgccgtag acgtcattca tgaagccttg actcccgccc gggaccggct
      421 ttttgcggac ctcatggccg ccaaccccgt ggggatggcg ggactggcgg ccagcgactt
      481 ctctcccacc agcattttcc tcgacccagg ccgaatcgat gtgtcggcgg ccgtggatcg
      541 caaccaccgg ggtgtgcttg acgcccgata cgatgtcgag ctatttgaac gttacgatga
      601 tgacattctc atcggcggcg ccggcgacga ctggctcctc ggcggcaagg gcgacgacct
      661 cctgatcggc ggtgccggca acgacaccta catctaccgc gagggcgacg gcgacgaccg
      721 gatcatcgat tcggacaaga gcggccggat catcgtcgtc agcccggaca accgatacga
      781 gtacgccgcc ggcaccttcc tcaaggaccc gggcggcagc gagctctgga tcaacctcga
      841 cgaccacatc cggattaccc acaacagccc ctggaagctg gtcctggccg atgggtcgga
      901 gatccagttg ggcgaggact tcgccgacgg cgactacggc atcaacctgg aggactacag
      961 taccgccatc gtcccggccc tgaccgtggc cggtgacctg gcgccggtgg accgggaccc
     1021 tgccaccccc ggcattcagg tcggctacga cgcctgcggc aacctcatcg tcggcggcgc
     1081 cgccgaacct ggccgggccg accgcctcta cggctcgggt ctctcggacc aggtcactgc
     1141 cggcg
//
LOCUS       DAOQMF010000724         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_115114_length_1145_cov_2.181651, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000724 DAOQMF010000000
VERSION     DAOQMF010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115114_length_1145_cov_2.181651"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLV77_10580"
     CDS             complement(<1..227)
                     /locus_tag="PLV77_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16338.1"
                     /translation="MGKKKSKSKKQKQTLAELQQQAVEQSVEILREKPGYKTLERETV
                     DFGSFEYPLDGVNSTGSRMVEINAHAGKLESV"
     gene            complement(292..672)
                     /locus_tag="PLV77_10585"
     CDS             complement(292..672)
                     /locus_tag="PLV77_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16339.1"
                     /translation="MKRLIGVAVLGAALLLAGCGGDSDEDQAGEVVSNFAAALADGDY
                     AAACEMYSPKTHEFFKDTSDVTGGCEPGIERVYSGLSHEKLEAYGDVEEVTIDGDKAT
                     VEQASGDYTILQKVDGEWMLTLDQ"
     gene            complement(729..>1145)
                     /locus_tag="PLV77_10590"
     CDS             complement(729..>1145)
                     /locus_tag="PLV77_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16340.1"
                     /translation="AKKWSPRKYQVRYWSLCSGSSPVTGLGYDCVYDQQVPLRGNRRY
                     TIVVGKAKDRPANATRACGYSWINFGKGENYDDPASRDYVDTMYMRFMAVDPTWKQAP
                     QKVKTPGTEKKVMGPYFPTSKYTTKAAFQKLGCKKK"
BASE COUNT          185 a          375 c          330 g          255 t
ORIGIN      
        1 acgctttcga gcttgccggc gtgggcgttg atctcgacca tcctgcttcc ggtcgagttg
       61 accccgtcaa gcgggtactc gaaactcccg aagtcgacgg tctccctttc gagcgtcttg
      121 tagccaggct tctcgcgaag gatctccacc gactgctcga cggcctgctg ctggagctcg
      181 gccagggtct gcttctgctt cttgcttttc gacttcttct tgcccattcg cagacagact
      241 agctagccgg agacctcgaa gagcgccggt tcccggacgg cccccggacc gctactgatc
      301 gagggtcagc atccattcgc cgtcaacctt ctggaggatc gtgtaatcac cgctggcctg
      361 ctcgaccgtg gccttgtcac cgtcgatcgt gacctcctcg acatcgccgt acgcttcgag
      421 tttctcgtga ctgagccccg agtacacccg ttcgatgccg ggttcgcagc cgcccgtcac
      481 gtcgctcgtg tccttgaaga actcgtgggt cttcggcgag tacatctcgc aggctgccgc
      541 atagtcaccg tcggcgagcg cggcagcgaa gttcgagacc acctcgcccg cctgatcttc
      601 atccgagtcg ccaccgcatc ctgccagcag caacgccgcg ccgagaaccg caaccccgat
      661 caacctcttc aagtttcctc ctggacgaga ctggtgtttc caccatatcc gcgggagggt
      721 catgggagtt acttcttctt gcagccgagc ttctggaagg cggcctttgt cgtgtatttc
      781 gaggtcggga agtagggacc catgaccttt ttctcggttc ccggagtctt cactttctgg
      841 ggggcctgct tccaggtcgg gtcaaccgcc atgaagcgca tgtacatggt gtcgacgtaa
      901 tcgcgcgaag ccggatcatc gtagttttcg cccttgccga aattgatcca gctgtagccg
      961 caggcccgcg tggcattggc cggacggtcc ttcgccttgc cgaccacgat cgtgtagcgg
     1021 cgattcccgc gcaacggaac ctgctggtca tagacgcagt cgtagccgag gccggtgacc
     1081 ggcgaggagc cgctgcagag cgaccagtag cggacctggt acttgcgggg actccacttc
     1141 ttcgc
//
LOCUS       DAOQMF010000725         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_115458_length_1143_cov_3.229779, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000725 DAOQMF010000000
VERSION     DAOQMF010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115458_length_1143_cov_3.229779"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..217)
                     /locus_tag="PLV77_10595"
                     /pseudo
     CDS             complement(83..217)
                     /locus_tag="PLV77_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(373..>1143)
                     /locus_tag="PLV77_10600"
     CDS             complement(373..>1143)
                     /locus_tag="PLV77_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNC16341.1"
                     /translation="QNPGGVTLSLERRRRLVEIAREKEILVVEDNPYGLLRFEGEPLP
                     TLYSLDGGNFVVYLGTFSKILSAGLRIGWLVTPNPIREKVVLGKQAADLCTSTLTQDF
                     AGHFFQTGRWREYVAELTGIYRARRDVMISAMAEYFPHGATWNKPQGGLFIWATMPDV
                     LSSEDLLAKSLSRNVAFVPGPNAYVDGRGVSSMRLNFSGVREDEIIEGIRRIGQVARE
                     MLKLYETMTSTDEMKLPPPESTDGGGSEMKLPPREDLP"
BASE COUNT          201 a          360 c          373 g          209 t
ORIGIN      
        1 cggcaccgaa atcacggatc gacgcctcgc tgtaggtgac ccagtccggg gtgggcagat
       61 catgctcgcg caggatgtac ttggtcaggc tcttgtccat gcagcgtgtg caggcggcga
      121 ccccgggccc ggtgtagggg atgccgagca gttccagcag ggcctggatc gtgccgtcct
      181 cgccctcggt gccgtgcagg gcgatgaaga ccacgtccgg acgctttgct tcgagccggt
      241 tgaccagatc cgggccgagg tccatcacat ccacctcgtg gccgaggtcg agcagggatt
      301 cggtaacccg cattccggat cgaagtgaaa cgccccgctc gagcgagcgg ccgccctgaa
      361 gcagcgcgac tttcaaggaa ggtcctctcg tggcggaagc ttcatttcgc ttccgcctcc
      421 atcagtggac tccggtggcg gaagcttcat ttcatcggtt gaagtcatcg tctcgtagag
      481 cttcagcatc tcgcgggcta cctggccgat gcggcggatc ccctcgatga tctcgtcttc
      541 cctgactccg gagaagttca gccgcatcga gctgaccccg cggccgtcaa cgtaggcatt
      601 cggtccgggc acgaaagcga cattgcgcga aagcgacttc gccagcaggt cctcgctgct
      661 cagcacgtca ggcatggtcg cccagatgaa gaggcctccc tgcggcttgt tccaggtcgc
      721 gccgtggggg aaatactcgg ccatggcgct gatcatcaca tcgcgccggg cccggtagat
      781 gccggtcagc tcggccacgt actcgcgcca gcggccggtc tggaaaaaat gcccggcgaa
      841 gtcctgggtc agggtggaag tgcagagatc ggcggcctgc ttgccgagca cgactttctc
      901 gcgaatcgga ttgggagtga ccagccagcc gatcctgagg ccggccgaga ggatcttcga
      961 gaaagtgccg agatagacca caaagttgcc gccgtcgagg ctgtagagcg tgggcagggg
     1021 ttcgccctcg aaacggagca gtccgtacgg gttgtcctcg acgaccagga tctctttttc
     1081 gcgggcgatc tcgaccagcc gccggcgccg ttccagcgag agcgtcaccc cgcccggatt
     1141 ctg
//
LOCUS       DAOQMF010000726         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_115528_length_1143_cov_2.245404, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000726 DAOQMF010000000
VERSION     DAOQMF010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115528_length_1143_cov_2.245404"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..>1143)
                     /locus_tag="PLV77_10605"
     CDS             complement(88..>1143)
                     /locus_tag="PLV77_10605"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HNC16342.1"
                     /translation="HTLSEEATDLYEGARAKVAGLLGSTSPREVVFTRNVTSALNLVA
                     QSWGTANLQAGDRILLTEMEHHANIVPWHMIAQRTGAELQFAPIDEEGRLDRAALSEL
                     LAGGPRVFAFTHVSNVLGTKNPVTELVAEAKAAGALTVIDGAQSVPKFPVNVAEIGAD
                     FYGFTAHKLYGPTGIGAIWGRRELWEALEPFEGGGSMINKVTPEKITWASVPARFEAG
                     TPPIAEAAGLAAAIDWVQEVGIRAIEAHENQLTAYALPRLAEVPGLTVFGPQDTVERE
                     GIISFELEGVHPHDVSEILDRHGVAVRAGHHCAQILMRRLGVAATTRASFAVYNTTEE
                     IDTLVEALHDARRIFGL"
BASE COUNT          180 a          362 c          411 g          190 t
ORIGIN      
        1 gacggaaatg acgagcgagg gcgtgattcg acgttgagac atgcccttgg cttacaccgc
       61 cgggacggtc ctgtcaacgc caaccgttca gaggccgaag atgcggcggg cgtcatggag
      121 ggcttcgacc agggtgtcga tttcctccgt ggtgttgtaa acggcgaagc tggcgcgggt
      181 cgtggcggcg acgccgaggc ggcgcatcag gatctgggcg cagtgatgac cggcccgcac
      241 cgcgactccg tgccggtcga ggatctcgga gacgtcatgg gggtggactc cctccagttc
      301 gaaggagatg atgccctcac gctcgaccgt gtcctgcggg ccgaaaaccg tcagcccggg
      361 aacctcggca aggcggggca gggcgtaggc ggtgagctgg ttctcgtggg cctcgatcgc
      421 ccggatcccg acctcctgaa cccagtcgat cgcggcggca agcccggccg cctcggcgat
      481 cggcggggtg ccggcctcga acctcgccgg gaccgaagcc caggtgatct tctccggggt
      541 gaccttgttg atcatcgagc cgccgccctc gaaaggctcc agcgcctccc agagttcgcg
      601 gcggccccag atcgctccga tcccggtcgg gccgtaaagc ttgtgagcgg tgaatccgta
      661 gaaatcggca ccgatctcgg ccacgttgac cgggaacttc ggaaccgact gggcgccatc
      721 gatcacggtg agtgccccgg ccgccttcgc ctcggcgacc agttcggtca ccgggttctt
      781 ggtgccgagt acgttcgaga catgggtgaa ggcgaagacc ctggggccac cggccagcag
      841 ttccgatagc gcggcccggt caagccggcc ctcctcgtcg atcggcgcga actgaagctc
      901 ggcaccggtc cgctgggcga tcatgtgcca gggcacgatg tttgcgtggt gctccatctc
      961 ggtcagcagg atgcggtcgc ccgcctggag gttggccgtg ccccaggact gggcgacgag
     1021 gttgagggcc gaggtcacat tgcgggtgaa gaccacctcg cgcggggagg tcgatccgag
     1081 cagcccggcg acctttgcgc gcgcgccctc gtagaggtcg gtggcttcct cggagagggt
     1141 gtg
//
LOCUS       DAOQMF010000727         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_115875_length_1141_cov_3.232965, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000727 DAOQMF010000000
VERSION     DAOQMF010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115875_length_1141_cov_3.232965"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLV77_10610"
     CDS             <1..463
                     /locus_tag="PLV77_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNC16343.1"
                     /translation="RTLLPDAPELLRAAEELVGDGFIVLPYTNDDPILAARLEAAGCA
                     AVMPLGGPIGSGAGIRNPYNISIIVENAKVPVILDAGIGTASDATLAMELGCDAVLLA
                     SAVSRAEKPDLMAVAMKHAVEAGRAARQAGRIPKRLYAEASTPEEGLPGLG"
     gene            520..>1141
                     /locus_tag="PLV77_10615"
     CDS             520..>1141
                     /locus_tag="PLV77_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNC16344.1"
                     /translation="MNVDVTTRGSVDPEAVELAQEKLTELESAVGRPLSHVRVVLRQE
                     KESIPNAARAEGEAQVGKTIIRGHVAADSMSHAVHELADRLQQQIRRHLDRVTSLKRQ
                     PVETPDGKWDHGTWVPARPAQSLLAPGEREVIRRKVLSLVPLKASEAAELMRELDHGF
                     YLFHDSDTEADSVIYLRDDGQLAVIAAQGVAAPADGEDSSGIQRETS"
BASE COUNT          202 a          393 c          379 g          167 t
ORIGIN      
        1 tcgcaccctg cttcccgacg ctcccgaact gctcagggcg gccgaggagc tggtcggtga
       61 cggcttcatc gtgctgcctt acaccaacga cgatccgatc ctcgccgccc ggctggaggc
      121 ggccggctgc gccgcggtga tgccgctggg cgggccgatc ggatcgggcg cggggatccg
      181 caatccgtac aacatctcga tcatcgtcga gaacgcaaag gtgccggtga tcctcgacgc
      241 cgggatcggc accgcctccg acgccaccct ggcgatggaa ctcggctgcg acgcggtgct
      301 cctggccagc gcggtttccc gggccgaaaa gccggacctg atggcggttg ccatgaaaca
      361 cgcggttgaa gcgggccgcg ccgcccgcca ggccggccgg atcccgaagc gcctttacgc
      421 cgaagccagc acccccgaag aaggcctccc cggcctcggc taagtccccc gcatgaaggt
      481 gcccctcggg gattcgaacg cgtcacctat gatctggcga tgaacgtcga cgttacgacc
      541 cgtggttcag ttgatcccga agcagtcgag ctggcccagg agaaactcac tgaactcgag
      601 tccgccgtcg gccgtccgct gagtcacgtc cgggtggtgc tgcggcagga gaaggagagc
      661 atcccgaacg ccgcccgggc cgagggcgaa gcgcaggtgg gcaagacgat cattcggggc
      721 cacgtggccg cggacagcat gtcccacgcg gttcacgagc tggccgaccg gcttcagcag
      781 cagatccggc ggcacctcga tcgggtcacc agcctcaagc gccagccggt cgagaccccg
      841 gacggcaagt gggatcacgg aacatgggta ccggcccggc cggctcagtc attgcttgct
      901 cccggagagc gggaagtcat ccggcgcaag gtcctttcac tcgtgccgct gaaggcctcc
      961 gaggcggcgg aactgatgag ggagctggac catggcttct acctcttcca cgactcggat
     1021 accgaggccg attcggtcat ctacctccgg gacgatggcc aactcgcggt gatcgccgca
     1081 cagggcgtcg ccgcccccgc ggacggggaa gactcatccg gaatccagcg tgaaaccagc
     1141 c
//
LOCUS       DAOQMF010000728         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_116287_length_1139_cov_3.463100, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000728 DAOQMF010000000
VERSION     DAOQMF010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116287_length_1139_cov_3.463100"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..>1139)
                     /locus_tag="PLV77_10620"
     CDS             complement(213..>1139)
                     /locus_tag="PLV77_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16345.1"
                     /translation="QFQLVKDYDVTSVVDPATERMNSALPDFQGDIWFVVKQTGEVGT
                     VDRKTGQVRYLRLGVGIQNSFTVDRNAVYVVTSKRIYRMWKNGNGKPYVVWSAKYRNT
                     GVQKPGQADAGTGTTPDVMSGGYVSMVDNANPENIVVYRTAAKLHGKKRKVCEVPVFG
                     KDASATENSLITTGRSMIVENNYGYTNIFDPGAGAVVTRAGFARVDIRKDGKGCKKIW
                     TNKTERAPSVVPKMSAKTGLIYSYTRPPDPSGAQGYYWSAIDYRTGKTVWNKYAGSGF
                     LYNNNYSGLGLGPDGTAYLGVIGGIISLKDGS"
BASE COUNT          188 a          350 c          357 g          244 t
ORIGIN      
        1 catgtactcc ctcgaggact acacccagat caagcacgtg atgatcaata cggcttaact
       61 gcagtcgcgc ccgtcttcgc cggcgctcca gtcgaaaccc gcccgcccac tgaacaaatt
      121 tatggatggg cgggctccgt ttggcggtgg tacaaacccg gccgtcgagc atcgttactc
      181 gacggcctgg ggaacgctgg aactgattcg ggctagctgc cgtccttgag ggagatgatg
      241 ccgccgatca ctccgaggta ggcggtgccg tcggggccga gaccgagacc cgagtagttg
      301 ttgttgtaga ggaagccgga gcccgcgtac ttgttccaga ccgtcttgcc ggtgcggtag
      361 tcgatcgccg accagtagta accctgggcg ccgctcggat caggcggacg ggtgtaggag
      421 tagatcaggc cggtcttcgc tgacatcttc ggaaccactg aaggcgcccg ctcggtcttg
      481 ttggtccaga tcttcttgca gcccttgccg tccttgcgaa tgtcaacgcg ggcaaagccg
      541 gccctggtca ccacggcacc ggctccggga tcgaagatgt tcgtgtatcc gtagttgttc
      601 tcgacgatca tcgagcggcc ggtggtgatc agcgagttct cggtggcgct ggcgtccttg
      661 ccgaagaccg ggacctcgca gaccttccgc ttcttgccgt ggagtttcgc cgcggtccgg
      721 taaaccacga tgttctccgg attggcgttg tcgaccatgg atacgtagcc gccactcatc
      781 acgtcgggag tcgttccggt gccggcgtcg gcctgtccgg gcttctggac gccggtgttc
      841 cggtacttcg cgctccagac cacatagggc ttgccgttgc cgttcttcca catccggtag
      901 atccgcttgc tggtcactac atagaccgcg ttccggtcga cggtgaacga gttctggatc
      961 cccacgccga gcctgaggta cctgacctgg ccggtcttgc gatcgaccgt gccgacctcg
     1021 cccgtctgct tgacgacgaa ccagatgtca ccctggaaat cgggcagggc cgagttcatg
     1081 cgttcggttg cggggtcgac gaccgaggtc acgtcgtagt cctttacgag ctggaactg
//
LOCUS       DAOQMF010000729         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_116393_length_1139_cov_2.071033, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000729 DAOQMF010000000
VERSION     DAOQMF010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116393_length_1139_cov_2.071033"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1139)
                     /locus_tag="PLV77_10625"
     CDS             complement(<1..>1139)
                     /locus_tag="PLV77_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC16346.1"
                     /translation="DWHRRAEQIELEGRAFIDGSHADAISGGQMETVKPRDGSVLARI
                     AACDAADVDAAVSAARRSFESGQWSEAHPRVRKRVLHELVDRIQADREEIGLLISLEM
                     GKPITDAIGEVDSSCRELAFFAEAVDKVYGNTVPTDAKSLGMSVREPKGVIGAVTPWN
                     FPLMMPIYKVAPALAAGNSIVLKPAEQSSLAAIRLAELASEAGLPDGVLNVVPGTGEK
                     AGRSLGLHMDVDAITFTGSTSIGKAFLAYSSESNMKGVWLECGGKSPNVVLADAPDLD
                     RAAEFAAETIFHGAGQVCNAGSRLIVERPIYEDFVSRVARESLKWAPADPLDASAKMG
                     PLVDRPALDRALGYVSAAKSDGATVVSGGGETLDEHVPHDDEEGV"
BASE COUNT          188 a          390 c          356 g          205 t
ORIGIN      
        1 gtacgccctc ttcgtcatcg tgggggacat gctcgtcgag ggtctcgcct ccgccgctga
       61 ccacggtcgc cccgtcggac tttgcggcgg acacgtaacc gagagcccgg tcgagggccg
      121 gccggtcgac cagtgggccc atcttcgccg aggcgtcgag cgggtctgcc ggcgcccact
      181 tgaggctttc ccgggcaact cgacttacga agtcttcata gatcggtcgc tcgactatga
      241 gtcgggatcc cgcgttgcag acctgaccgg caccgtggaa gatcgtctcc gcggcaaact
      301 ctgcggcccg gtcgaggtcg ggcgcatcgg ccagcaccac gttgggagac ttcccgccgc
      361 attccagcca gacgcctttc atgttcgact cggatgaata ggcgaggaag gccttgccga
      421 tcgaagtcga accggtgaac gtgatcgcgt cgacgtccat gtggaggccc agcgaccgcc
      481 cggccttctc gccggttccc ggcacgacgt tgaggacgcc gtccggaaga ccggcttcgg
      541 aggcaagctc ggccagcctg atcgccgcca gcgaagattg ctcggccggt ttcagcacga
      601 tcgagtttcc agccgcgagc gcgggagcga ccttgtagat cggcatcatc agggggaagt
      661 tccagggcgt gacggccccg atcactccct tcggctcgcg caccgacatg ccgagtgatt
      721 tcgcatcggt gggaaccgta tttccgtaaa ccttgtccac cgcttcggcg aagaaggcga
      781 gctcgcgaca ggaggaatcc acttcaccga tcgcgtcggt gatcggcttg cccatttcca
      841 gcgagatcaa cagaccgatc tcttcccggt cggcctggat ccgatcgacc agttcgtgga
      901 gcacgcgctt gcgtacccgg ggatgagctt cggaccactg gcccgactcg aacgacctgc
      961 gcgccgcgct gaccgccgca tccacgtcgg ccgcgtcgca tgcggcaatc ctggcgagca
     1021 cagacccgtc tctcggtttg acggtctcca tctgcccgcc cgaaatcgca tcggcgtgcg
     1081 agccgtcgat gaaggcgcgc ccctcgagct cgatctgctc cgccctcctg tgccaatcg
//
LOCUS       DAOQMF010000730         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_116491_length_1138_cov_3.944598, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000730 DAOQMF010000000
VERSION     DAOQMF010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116491_length_1138_cov_3.944598"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLV77_10630"
     CDS             complement(<1..823)
                     /locus_tag="PLV77_10630"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC16347.1"
                     /translation="MKWIVVAGVALACLLGMADASFGAGLKRITPEEYFGSDPVFLTS
                     PPGDSRLFVVERGDSSTHQGMVRIIENGQPLAEPFLTVDNVGLENERGLMSLAFAPDY
                     ATSGLFYVFYVVNESGDAFGGDGGDIRIVEYQVSAGDPDKADPASARLVFSTPHYTTY
                     HQGSWMAFGPDGHLYFTIGDDGYGPNAQLKDNYFGKIMRIDPADPDGNGPASHTVPAD
                     NPFVAEAGTRPEIYAYGLRNPFRASFAPDGRLVVGDVGNATWEEINAGYLKGANLG"
     gene            909..>1138
                     /locus_tag="PLV77_10635"
     CDS             909..>1138
                     /locus_tag="PLV77_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16348.1"
                     /translation="MFLGVACLPFFASMASAGLVRTDSWGGGLVDPGFVVQSVAYDSQ
                     GRAYIGESGKARVIRLAADGSVDLNWSVGQLPG"
BASE COUNT          187 a          360 c          374 g          217 t
ORIGIN      
        1 agccgaggtt ggcacccttc aggtagccgg cgttgatttc ctcccaggtg gcgttgccga
       61 cgtcaccgac caccagcctc ccgtcgggag caaacgaggc gcggaacggg ttgcgaagtc
      121 cgtaggcgta gatctccggc ctggtaccgg cctccgcgac gaacgggttg tcggcgggca
      181 ccgtgtgact ggccggcccg ttcccgtcag ggtcggccgg gtcgatccgc atgatcttgc
      241 cgaagtagtt gtcctttagc tgggcgttcg ggccgtatcc gtcgtcgccg atcgtgaagt
      301 agaggtggcc gtccgggccg aaggccatcc agctgccctg gtgataggtc gtgtaatggg
      361 gggtgctgaa cacgagccgg gccgaggccg ggtctgcctt gtccgggtca cccgccgaga
      421 cctggtactc gacgattctg atgtcaccgc catcgccgcc gaaggcgtcg cccgactcgt
      481 tgaccacgta gaagacgtag aagagcccgc tggttgcata gtccggggcg aaggccagtg
      541 acatcaggcc gcgctcgttt tcgaggccga cgttgtccac ggtcagaaag ggctcggcca
      601 ggggctggcc gttctcgatg atccgcacca tcccctgatg ggtcgagctg tcaccccgct
      661 cgaccacgaa aagcctcgaa tcaccggggg gtgaagtcag aaataccggg tcggacccga
      721 aatactcctc cggagtgatc cgcttcagcc cggccccgaa tgacgcatcg gccattccga
      781 gcaggcaagc caaagccacc cctgcgacaa cgatccactt catgccacag aaactacatc
      841 gaggccgtcg ggttgtgatt tgtgtgaacc ggtttcgtcc gaaaggtccc cttcgcctgt
      901 tgctgcccgt gtttctcggg gtcgcctgcc tgccgttctt cgcctcaatg gcttcggccg
      961 gactggtcag aaccgactcc tggggcgggg gtctcgtcga tcccgggttc gtggtccagt
     1021 cggtcgccta tgacagccag ggtcgcgcct acatcgggga atccggcaag gcgagggtga
     1081 ttcgcctggc cgccgacggt tcggtcgacc tcaactggag tgtcgggcaa ctgcctgg
//
LOCUS       DAOQMF010000731         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_116686_length_1137_cov_3.911275, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000731 DAOQMF010000000
VERSION     DAOQMF010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116686_length_1137_cov_3.911275"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1137
                     /locus_tag="PLV77_10640"
     CDS             68..>1137
                     /locus_tag="PLV77_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC16349.1"
                     /translation="MDPERSALASSQEAAVKAANDRLPERQREVLALRELDGLSYDEI
                     ASVMEMKSNAVAQLISRARLGLRKEMRVGAAASVAVASPECERAHGEMACRMDGQVKG
                     FDDEWLELHLAGCSNCRVASEEMAEAGVSYRAWAPVVPAAWLFREVMAKASGINGHDW
                     SGIERPEPADGAGSSDADGEGPETGGASGAVEAGRSSRARRALIAGAGSVLVAAVLAT
                     VLLAEANAPEKVAVVRDSLVRSKFKPEKASAKETKKGKAHARKANRNGQGPGGEQTGE
                     QTGPIDPASTDPSGDGGSGGGGNGPRSNGNTTGGGQNPSGSSGSDPLPTPPEPADPVV
                     PSNPTTPVPTDPTPDPPPTPDPP"
BASE COUNT          194 a          385 c          414 g          144 t
ORIGIN      
        1 cgatctttcc ggggcggctc cggatgaggc gtcggcgcct ttccgcgggg agctttcgga
       61 tctcgagatg gacccggagc gctccgcgct cgcctcctcg caggaggctg cggtcaaggc
      121 ggccaacgac cgcttgcccg agcgccagcg cgaggtcctc gccttgcggg aactggacgg
      181 cctgagctac gacgagatcg cctcggtcat ggagatgaag tcgaacgcgg tcgcccagct
      241 gatctccagg gcccggctcg gcctgcgcaa ggagatgcgg gtcggtgcgg ccgcctccgt
      301 cgcggtcgca tccccggaat gcgagcgggc ccacggcgaa atggcatgcc ggatggacgg
      361 tcaggtcaag ggcttcgacg acgagtggct cgaactccat ctggccggct gctcgaattg
      421 ccgggttgcg agcgaagaga tggccgaagc cggggtttcc taccgggcct gggcaccggt
      481 cgtcccggcc gcctggctgt ttcgcgaagt catggccaag gccagcggga tcaacggcca
      541 cgactggtcc ggaatcgagc ggccggagcc ggccgacggt gccggcagta gcgacgcgga
      601 tggcgaaggg ccggaaacgg gaggcgcgtc cggcgcggtc gaggccggac ggagcagccg
      661 tgcgcgaagg gcgttgatcg ccggcgccgg ttcggttctg gtcgctgccg tcctcgcgac
      721 cgtcctgctg gccgaagcca acgcgccgga gaaggtggcc gtggttcgag actcgctggt
      781 caggtcgaaa ttcaaaccgg aaaaggccag cgcgaaggaa acgaagaagg gcaaggctca
      841 cgccaggaag gccaacagga acggtcaggg cccgggcggg gagcagaccg gtgaacagac
      901 cggaccgatc gacccggcct caaccgaccc gtcgggtgac ggcggcagcg gaggtggcgg
      961 aaacggcccg cgctcgaatg gcaacacgac gggcggcggg cagaacccct ccggttcctc
     1021 gggatccgac ccgcttccaa ctcctccgga accagccgac ccggtcgtgc cctccaaccc
     1081 gaccactccg gtgccgaccg atccgacccc ggacccgccg ccgacccccg acccccc
//
LOCUS       DAOQMF010000732         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_116783_length_1137_cov_2.340111, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000732 DAOQMF010000000
VERSION     DAOQMF010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116783_length_1137_cov_2.340111"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1137
                     /locus_tag="PLV77_10645"
     CDS             <1..>1137
                     /locus_tag="PLV77_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC16350.1"
                     /translation="FIGKLLARGESGYEEARREALWNAFTPERYPEIIVQAVAEEDCV
                     AAVRLARRQGLKLSVRSGGHSWAGNHLRDGTLLLDLSRMWEVKVDGLNMRATAQPGCP
                     GNELDLELEKLGLFFPVGHCPGVAIGGYLLQGGFGWNGRVHGPACQSVVAIDLVTPEG
                     ELLTVSEENEPDLFWAARGSGPGFFSVVTRYHLRLYPRPPVIANGIATYPLGALEEVF
                     TWAREIGPRVPRWMELMVFTHLDQGEPEVVVTAPVLAPSESLAREALGLLETCPAIDR
                     AKISMPYFPARLEDLYAGVHASYPDGHRYAVDNMWTHAPVADLLPGIRRIAETLPEAP
                     SHMLWMNWGESPAREEMAYSVEDEIYIALYAVWQDPAGDEENVE"
BASE COUNT          180 a          394 c          379 g          184 t
ORIGIN      
        1 agttcatcgg gaaacttctg gcaagaggcg agtccggtta cgaggaagcg cggcgggaag
       61 ctctctggaa cgcatttacc ccggagcgct atcccgaaat catcgttcag gctgtggccg
      121 aggaggattg cgtcgcggcg gtccggctcg cccgccggca gggtctgaag ctcagtgtcc
      181 gttccggagg tcacagctgg gccggcaacc acctccgtga cggaaccctg ctgctcgacc
      241 tttcccggat gtgggaagtg aaggtggacg ggctgaacat gcgggccacg gcccagcccg
      301 gatgccccgg caacgagctc gacctcgagc tggagaagct cggcctcttc ttcccggtcg
      361 gccactgccc cggagtggcg atcggcggct atctgctgca gggcggcttc ggctggaacg
      421 gccgggtcca cgggcccgcc tgccagagcg tcgtggcgat tgacctggtc accccggaag
      481 gcgagctgct cacggtcagc gaggagaacg agcccgacct gttctgggcg gcgcgcggct
      541 ccgggcccgg cttcttttcg gtggtcaccc gctaccacct gcggctctac ccgcgacccc
      601 cggtaatcgc caacggcatc gcgacctacc cgctgggcgc cctcgaggaa gtcttcacct
      661 gggccaggga gatcggtccc cgggtgccgc gctggatgga gctgatggtc ttcacccatc
      721 ttgaccaggg cgagcccgag gtcgtggtca ccgccccggt tctggcgccc tccgaatcgc
      781 tggcccggga agcgctcggc ctgctggaaa cctgtccggc gatcgaccgc gcgaagatct
      841 cgatgcccta cttcccggcc cggctcgagg acctctacgc cggagttcac gcctcctacc
      901 ccgacggtca ccgctacgcg gtcgacaaca tgtggaccca cgccccggtc gccgatcttc
      961 tgcccgggat ccggcggatc gccgagaccc ttcccgaggc gccatcccac atgctctgga
     1021 tgaactgggg tgagagcccg gcccgcgagg agatggccta cagcgtcgag gacgagatct
     1081 acatcgccct gtacgccgtc tggcaggacc cggccggcga cgaagagaac gtcgagt
//
LOCUS       DAOQMF010000733         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_117570_length_1133_cov_2.776438, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000733 DAOQMF010000000
VERSION     DAOQMF010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117570_length_1133_cov_2.776438"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLV77_10650"
     CDS             complement(<1..425)
                     /locus_tag="PLV77_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC16351.1"
                     /translation="MISVVEPATEQVMAELPRAGVEEVDAAVAAARAAFPAWRAVTPG
                     DRATLMRRIADGVEARLEEIATLEARNAGKPISDARGEIGMVVETFRYYAGAPERLLG
                     HTIPVAGGVDFTFREPMGVIGLIVPWNFPLAITSWKVAPA"
     gene            complement(425..>1133)
                     /locus_tag="PLV77_10655"
     CDS             complement(425..>1133)
                     /locus_tag="PLV77_10655"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNC16352.1"
                     /translation="IGVTAATEPARWGAWNQLADLAPHDYSVAVQRAGGIALLLPTDR
                     AVTEDPGPLLDRLDGLILGGGADVDPGSYESEAGPLTAGTRPERDQFELALTRGALER
                     DLPLLGVCRGFQLMNVAAGGTLEQHLPDVVGHERHRSVPGEFDQHEVELAAGSRIARI
                     CGKDRLTVHSHHHQGLARIAEAIEVTGVAVPDRVTEAIELPGREFALGGQWHPEVDED
                     DSLIAALVEAARGRMSA"
BASE COUNT          159 a          398 c          400 g          176 t
ORIGIN      
        1 gccggggcga ccttccagga ggtgatcgcc agcgggaagt tccagggcac gatcaggccg
       61 atcacgccca tcggctcacg gaaggtgaag tcgactcctc cggcgaccgg gatggtgtgg
      121 ccgagcagcc gctcgggtgc gcccgcgtag tagcggaagg tctcgacgac catgccgatc
      181 tcgcccctgg cgtcgctgat cggtttgccg gcgttcctgg cctcgagggt cgcgatctcc
      241 tccagccggg cttcgacccc gtcggcgatt ctgcgcatca gcgtcgcccg atcgccgggg
      301 gtgaccgcgc gccaggcagg gaaggcggcg cgggcggcgg ccacggcggc gtcgacttct
      361 tcgactccgg cccggggcag ttcggccatg acctgttcgg ttgcgggttc gactacggaa
      421 atcatcaggc gctcattctg ccacgggcgg cctcgaccag agccgcgatc agcgaatcgt
      481 cttcgtcgac ttcggggtgc cactggcccc ccagcgcgaa ctcccttccg ggcagctcga
      541 tcgcttccgt gacccgatcc ggaacggcga cgccggtcac ttcgattgcc tcggcgatcc
      601 gggccagacc ctgatggtga tgggagtgga cggtcagccg gtccttgcca cagatccgcg
      661 cgatccgcga gccggcggcc agctcgacct cgtgctggtc gaactcgccc ggaaccgagc
      721 ggtgccgctc gtggccgacg acatcgggga ggtgctgctc aagcgttccg cccgccgcca
      781 cgttcatcaa ctggaaaccc cggcagaccc cgaggagcgg caggtcccgc tcgagtgcgc
      841 cgcgggtcag ggcaagctcg aactgatccc gctcgggtct ggtgccggcg gtaagcggtc
      901 ccgcttcgga ctcgtaggag ccggggtcga catcggcgcc gccccccagg atcaggccgt
      961 cgagacggtc gagcagcgga ccgggatctt cggtgaccgc ccgatcggtc ggcaggagca
     1021 gggcgatccc tccggcccgc tggaccgcca ccgagtagtc gtgcggtgcc aggtcggcga
     1081 gctggttcca ggcgccccac cgtgccggtt cggtcgcggc cgtgaccccg atc
//
LOCUS       DAOQMF010000734         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_117762_length_1132_cov_3.176416, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000734 DAOQMF010000000
VERSION     DAOQMF010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117762_length_1132_cov_3.176416"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLV77_10660"
     CDS             <1..634
                     /locus_tag="PLV77_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC16353.1"
                     /translation="VEGIATAGYFNAGQDCTAATRILAGPKVYDDVVNGLAGEAKGYV
                     MGDLDSAETTLGPVNSARQLEHVTGFFDRAPSHAQIVTGGKQAEGLPGFFVEPTVVAD
                     LRQDDEMVQNEIFGPAMTVQRFTDEEEAIRWANGTKYGLASSVWTRDVGRAMRVTNAL
                     RFGAVWVNDHILMAVEMPHGGYNESGYGKDMSMYSLEDYTQIKHVMINNG"
     gene            892..1095
                     /locus_tag="PLV77_10665"
     CDS             892..1095
                     /locus_tag="PLV77_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16354.1"
                     /translation="MDFRKLGQQAGKLKKQAEDKLGDRAEPENLKRDAKELREILAGD
                     GSLAEKAKQAKKEVVDRDKPVGN"
BASE COUNT          239 a          355 c          365 g          173 t
ORIGIN      
        1 ggttgagggc atcgccacgg ctggttactt caacgccggt caggactgca ccgcggcaac
       61 ccgcatcctg gcggggccca aggtctacga cgacgtggtc aacgggctag ccggcgaggc
      121 gaagggctac gtgatgggtg atctcgattc cgccgagacc acgctcggcc cggtcaactc
      181 ggcccggcag ctggaacacg tgaccggctt cttcgaccgc gcgccctcgc atgcccagat
      241 cgtcaccggt ggcaagcagg ccgaaggtct gcccgggttc ttcgtcgagc cgaccgtggt
      301 cgccgacctc cggcaggacg acgagatggt ccagaacgag atcttcgggc cggcgatgac
      361 cgtgcagcgc ttcaccgacg aggaagaagc gatcaggtgg gccaacggca ccaagtacgg
      421 cctggcctcc tcggtctgga cccgtgatgt cggtcgcgca atgcgggtca ccaacgcgct
      481 ccgcttcggt gccgtctggg tgaacgacca catcctgatg gcggtcgaga tgccccacgg
      541 cggatacaac gaatccggct acggcaagga catgagcatg tactccctcg aggactacac
      601 ccagatcaag cacgtgatga tcaataacgg gtagcccgag cggcgccagg aaccaccagc
      661 tgcaacacaa ggaacatccg gctcgttcgc tcgatggtcg ttcaatgacg gtctcgctga
      721 acggccggat ggcttcgtcg acggccggaa acggcagctc aagtacccac gtacgatcgc
      781 ggccatttcc ggcctgtctc ctcggcagcg aacggctgga ggcccctggc atccacccgc
      841 tgagagtctg caagggcaac ttccgggttg tccttttgct ttgatgggcg tatggacttc
      901 aggaaacttg gtcagcaggc cgggaagttg aagaaacagg ctgaggacaa gctgggggac
      961 cgggccgagc ccgagaacct gaagcgggac gccaaggagc tacgcgagat cctcgccggc
     1021 gacggctcac tcgccgaaaa ggcaaaacag gcaaagaaag aagtcgtaga ccgggacaag
     1081 ccggtgggca attaacccga tgcgccgccg gacggagccc ctcccaacaa tt
//
LOCUS       DAOQMF010000735         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_118162_length_1130_cov_3.463256, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000735 DAOQMF010000000
VERSION     DAOQMF010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118162_length_1130_cov_3.463256"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLV77_10670"
     CDS             complement(<1..572)
                     /locus_tag="PLV77_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16355.1"
                     /translation="MADSKGRSLNAKRGGRARSLHDLAAHDPRLLDPSSTKTRKEVPT
                     DPEATEEAGHLIGRQSADAGDDPTASVAETPVVEETPAENTATDLDTGEIVVDEPDLD
                     PEPEAGPAAVQAVRRPPRADFRDALQLMSWTTTQDLWDRYSSLALTLRKQTGVKVKTT
                     QLIRAVVEHNFPDTSTKSGVSKLAGYAERW"
     gene            complement(565..>1130)
                     /locus_tag="PLV77_10675"
     CDS             complement(565..>1130)
                     /locus_tag="PLV77_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC16356.1"
                     /translation="PDIHELMDLLPAKPDGALRVRDALEDFLDSPAGRDFDFVFIDCP
                     PSKGIFPTSAMIAVDEIMAPVDLRTTDSLTGIFQLIRSFDELPARYQPADGIFLVGNL
                     FDEVGTDEAENRDALGNLGLPVASTVVKTRKAISKSQNRMSPLVEQVGEQSKGARKAY
                     KNISALAEEIRSGKIKGQKPVKEAVHG"
BASE COUNT          196 a          346 c          335 g          253 t
ORIGIN      
        1 ctccaccttt ccgcatagcc cgcaagcttc gacacgccgg acttcgtaga agtatcggga
       61 aagttgtgtt cgaccacggc ccggatcagc tgggtcgtct tgaccttcac cccggtctgc
      121 tttcgcaggg tcaacgcaag gctcgagtag cggtcccaga gatcctgggt tgtggtccat
      181 gacatgagct ggagggcatc gcggaaatcg gcccggggag gccttctcac agcctggacg
      241 gcagccggcc cagcttccgg ttcaggatcc aggtcgggtt cgtcgaccac aatttcgccg
      301 gtgtccagat ccgtcgcggt attttcggcg ggtgtttctt caacgaccgg tgtttcggcg
      361 acgctcgcag tcggatcgtc tccggcatct gcggactgcc tgccgatcaa atggccggct
      421 tcctcggtcg cctccgggtc ggtcggaacc tctttccggg ttttggtcga ggatggatcc
      481 agcaaccggg gatcatgagc ggccaggtca tgcagagacc ttgcgcgccc tccccgcttc
      541 gcgttcaaag acctgccctt gctgtcagcc atgaacagcc tccttcaccg gcttttgccc
      601 tttgattttc ccggacctga tttcttcggc cagggcggaa atgttcttgt aggccttcct
      661 cgcccccttc gactgctcgc cgacctgttc gaccaacgga ctcattcggt tttgtgactt
      721 cgagatcgct ttgcgcgttt tcaccacggt tgatgcgacc ggcaacccga gatttccaag
      781 cgcgtcacgg ttctcggcct cgtcggttcc gacctcatcg aaaaggtttc cgactaggaa
      841 aatcccgtcg gccggctggt atcgggcagg cagctcgtca aaggagcgga tcagctggaa
      901 aatgccggtc aacgagtcgg ttgtgcggag atcaaccggg gccatgatct cgtcgacagc
      961 gatcatcgcg gaggtaggga aaatgccttt gctcggaggg cagtcgatga atacgaaatc
     1021 gaagtccctt ccggccgggc tgtcaaggaa atcctcgagc gcatccctca ccctgagcgc
     1081 accgtccggc ttggccggca ggaggtccat cagttcgtga atgtcaggat
//
LOCUS       DAOQMF010000736         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_118540_length_1128_cov_4.351351, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000736 DAOQMF010000000
VERSION     DAOQMF010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118540_length_1128_cov_4.351351"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="PLV77_10680"
     CDS             <1..1090
                     /locus_tag="PLV77_10680"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNC16357.1"
                     /translation="EFRGCGDSDNVARQFYPGKATYSEAVPVSVTQGSDTPGIDADLA
                     PGGAVTGTLFPTESPAEVCAMSVKAYDSDGNLAGSFNSPGFLYTPVDYRIDQLITGNY
                     RLHFEQGCLAIFSMDPFWVSEYFNNRNSLVEATPVSVTEGSTTSQISAVLGSESTISG
                     TITDTEGAPLENICVEAADENGPAGIIAYSDPDGHYSLNALLGGSYTLKFSDCQGTNE
                     RIVTPEYFDDKATIEEAVPVSVSAGGLRSGINARLATAPRPVPTAKISKLKVKGPAFV
                     RKGREATFRLRITNSGDATATGVRIKGSGRGISARAPVGKIAASTTRTVRVRLRPSKA
                     GKTMVTFRVTSSNAGGRTTKHKINVRKK"
BASE COUNT          245 a          350 c          342 g          191 t
ORIGIN      
        1 cgaattccgg ggatgcggtg actccgacaa cgtggcccgg cagttctatc ccggcaaggc
       61 gacctactcg gaagccgtcc cggtatcggt aacccagggg tctgacaccc cggggattga
      121 cgccgacctg gcgccgggag gcgctgtcac cggcaccctg tttcccaccg agtcaccggc
      181 cgaggtttgc gcaatgtcgg tcaaggccta cgactccgac gggaatcttg ccggctcgtt
      241 caactccccg ggtttccttt acacgcccgt tgactacagg attgaccagc tcataaccgg
      301 gaactacagg ctccatttcg aacagggttg cctcgcgatc ttctcgatgg atccgttctg
      361 ggtttccgag tacttcaaca accggaattc cctcgtcgaa gccacaccgg tttcggtgac
      421 cgaaggttcg accacgtccc aaatcagcgc tgtactggga tcggaaagca cgatctcggg
      481 cacgatcacc gacaccgaag gtgctccgct cgagaacatc tgcgtcgagg ccgccgatga
      541 aaacggaccg gccgggatca tcgcttacag cgatccggac ggccattact cgttgaacgc
      601 gctgctcggc ggttcataca cattgaagtt cagtgactgc cagggcacga atgagcggat
      661 cgtgaccccc gagtacttcg acgacaaggc cacgattgag gaagccgtgc cggtatcggt
      721 ctccgccggc gggttgagat cgggcatcaa cgcccggctg gcaaccgcgc cgagaccagt
      781 gcccacggcg aaaatcagca agctgaaggt gaagggtccc gctttcgtca ggaagggccg
      841 ggaagcgaca ttcaggttgc ggatcaccaa ctccggtgac gccaccgcga ccggggtccg
      901 gatcaagggc agcggacgcg gcataagcgc cagggcgccg gtcggaaaga tcgccgcgtc
      961 gacgaccaga accgtcaggg tcaggctgag accgagcaag gccggcaaga cgatggtcac
     1021 cttccgggtc acttcatcga acgccggcgg caggaccaca aaacacaaga tcaacgtgag
     1081 gaaaaagtga aatccgttct ggcagcaact ttgacgattg cctgtgcc
//
LOCUS       DAOQMF010000737         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_120129_length_1121_cov_2.881801, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000737 DAOQMF010000000
VERSION     DAOQMF010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120129_length_1121_cov_2.881801"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PLV77_10685"
     CDS             <1..960
                     /locus_tag="PLV77_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16358.1"
                     /translation="RHWFLPGFGDGFGSGWILLGVLAITGLVLCLDRNSGPARRTGAA
                     VGLALVLAWLVAPTSASGPAGEPNGFVSGLRYLAPALAVCLALLGSGVGQRGGRARWL
                     VAGLLLILAPVTIAFGDGWDAKEWLVAIVAATVFWVVCLIALGVWRVRSKQHRLSTRS
                     SRKLFGWTAGATIAFLVLCGFGVQRYYFNHRYASPDFTIPALSESYVWANGQSGVKIG
                     TNATRQYPLWGEDLDNEVQFIGVKQPHAGFVEAGNCRDFIAAANQGDYEYLVLTLDRE
                     GQKLLVPRELTWVSRDPAVKNVIDADGGAVLALDGPLSPRSCR"
     gene            989..>1121
                     /locus_tag="PLV77_10690"
     CDS             989..>1121
                     /locus_tag="PLV77_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNC16359.1"
                     /translation="MELIRNFSIIAHIDHGKSTLADRILELTKAVDPLKMQAQVLDSM
                     "
BASE COUNT          160 a          401 c          353 g          207 t
ORIGIN      
        1 cgccactggt tcctgcccgg cttcggtgac ggcttcggct ccggctggat tctgctcgga
       61 gtgctggcga tcaccggact ggtgctctgc ctcgatcgga attccggccc ggcccgccgc
      121 accggggccg cggtcggcct cgccctggtc ctggcctggc tggtcgcccc gacctccgcc
      181 tccggccccg ccggggaacc gaacggattc gtctccggac tccggtacct ggccccggcg
      241 ctggcggtct gcctagcgct gctcggcagc ggcgtcggtc agcgcggcgg ccgggcccgc
      301 tggctcgtgg ccggtctgct gctcatcctg gccccggtga cgatcgcctt cggtgatggc
      361 tgggacgcca aggaatggct ggttgcgatc gtggcggcga cggtcttctg ggttgtctgt
      421 ctgatcgccc tcggggtctg gcgggtccgg tcgaaacagc acagactctc gacccgctct
      481 tcccggaaac ttttcggctg gaccgccggc gcgacgatcg cattcctcgt cctctgcggg
      541 ttcggggtcc agcgctatta cttcaaccac cgctacgcct cgccggattt cacgatcccg
      601 gccctctccg agtcatatgt ctgggcgaac ggccagagcg gagtgaagat cggcaccaac
      661 gcaacccgcc agtacccgct ttggggcgag gacctcgaca acgaggtcca gttcatcggc
      721 gtcaagcagc cccacgccgg cttcgtcgaa gccgggaact gtcgcgactt catcgccgcc
      781 gccaaccagg gcgattacga gtacctggtc ctcaccctcg accgcgaagg ccagaagctg
      841 ctcgtgcccc gggagctgac ctgggtttct cgggatcccg cggtcaaaaa cgtgatcgac
      901 gccgacggag gggcggtact cgccctcgac gggccactct cgccgcgatc ctgccgatag
      961 tcccctgcgg catactgctc tgtccctgat ggaactgatt cgcaacttct cgatcatcgc
     1021 ccacatcgac catggcaagt cgaccctggc cgaccgcatc ctcgaactga ccaaggccgt
     1081 ggacccgctg aaaatgcagg cccaggttct ggactcgatg g
//
LOCUS       DAOQMF010000738         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_120520_length_1119_cov_4.543233, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000738 DAOQMF010000000
VERSION     DAOQMF010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120520_length_1119_cov_4.543233"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..>1119
                     /locus_tag="PLV77_10695"
     CDS             116..>1119
                     /locus_tag="PLV77_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16360.1"
                     /translation="MTPSLASYIAGTLQIAVVVGAVWYGSWWLRRWIVPEFSGALARL
                     AELTIAFALIILSLELIGSVGLLKEVWITVACVVIPLIGAGLAWKFAPRDVEEIAAPA
                     VPTWAFLVAVAVASWCVAEWAFPTQLSLDQGMFGGDTTWYHMPAAARFIQDSSIIPLH
                     FTDALRLAVWFYPQNSELMHGALMGVMGSDWLSPVFNMLPLGIALLACWCVGRPYGVG
                     PATLVAGAMLLSAGVMIETQPGEGRNDIVCFAFLIAFVAFLINGHQRRAPSSGAVDEK
                     PDPNAPLLDKGPLILAGIAAGIAISIKVSMLAPVGVIFLGMILISGKGRRVTTALCLG
                     "
BASE COUNT          147 a          387 c          362 g          223 t
ORIGIN      
        1 caggtgccgg tggccggcgg ttcctccacc tgagccgccg gggatccgcc gggagccgtt
       61 tcgcccggcc ataaattagg attcccgcgg tgccaaccct gctcacagga cggccttgac
      121 cccgtcccta gcctcataca tcgccggaac gctccagatc gccgtcgtgg tcggcgccgt
      181 ctggtacgga tcctggtggc ttcggcgatg gatcgtgccc gaattctcgg gtgcgctggc
      241 gcgcctcgcc gagctgacga tcgccttcgc gctgatcatc ctctcgctcg agctgatcgg
      301 ttcggtcggc ttgctgaagg aggtctggat caccgtcgcc tgcgtcgtga tccccctgat
      361 cggcgccggc ctggcctgga agttcgcgcc gcgagacgtt gaggagattg ccgcgccagc
      421 ggtccccacc tgggccttcc tggtcgcggt cgcggtggcg tcctggtgtg tggcggaatg
      481 ggctttcccg acccagctca gcctcgacca gggcatgttc ggtggtgaca cgacctggta
      541 ccacatgccg gccgcggcga ggttcattca ggactcctcg atcatcccgc tccacttcac
      601 cgacgcgctg cggctggcgg tctggtttta cccccagaac tcggagctga tgcatggcgc
      661 cctgatgggc gtgatgggca gcgactggct ttccccggtc ttcaacatgc tccccctcgg
      721 gatcgccctg ctcgcctgct ggtgcgtggg ccggccttac ggggtagggc ccgccaccct
      781 ggttgccggc gcgatgctcc tttcggccgg ggtgatgatc gagacccagc ccggcgaggg
      841 tcgaaacgac atcgtctgct tcgccttcct gatcgccttc gtcgccttcc tgatcaacgg
      901 gcaccaaagg cgggcgccca gttccggagc ggtggacgaa aagcccgacc cgaacgctcc
      961 tctgcttgac aagggcccgc tgatcctcgc cggtattgcg gccggcatcg cgatctcgat
     1021 caaggtttcg atgctggcgc cggtcggcgt catcttcctc ggcatgatcc tgatcagcgg
     1081 caagggacgc cgggtcacga ccgcgctctg cctcggcat
//
LOCUS       DAOQMF010000739         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_120618_length_1119_cov_2.692669, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000739 DAOQMF010000000
VERSION     DAOQMF010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120618_length_1119_cov_2.692669"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLV77_10700"
     CDS             complement(<1..906)
                     /locus_tag="PLV77_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16361.1"
                     /translation="MRVERRVIRDNEGVKPAYLIHGSDSAKIDQARTRLRQRAESESG
                     AGGLEVFEPADGKGSPDADALAASIGSLSLMPGRRYLLADRIEKWGKNQSATVAEALL
                     AAPDETTVVLIARGKVPAGIAEAVKKVGGEDLSYEAPEGARLPVHLIESAAARGFELT
                     PEAARFMIARMGESLPRLGNELDRLALWAGPDGLVDVEDLEEMVTDTSETSNFALGDA
                     VISGDRREVIAVAERLLAQGVSAGSTVFSTSTSIRRAHKALVRLEAGEDPSRLERELG
                     VPPFVARRLISSLTGTTVESMREAAI"
     gene            complement(903..>1119)
                     /locus_tag="PLV77_10705"
     CDS             complement(903..>1119)
                     /locus_tag="PLV77_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16362.1"
                     /translation="SEDAGLPALLDESSPRLAVISVGEDNRYGHPAADALSELSADGV
                     ETLRTDRDGTVSIVLGAGPGYRVETGR"
BASE COUNT          159 a          409 c          362 g          189 t
ORIGIN      
        1 aatcgccgcc tcccgcatcg attcgaccgt ggttccggtc aacgagctga tcagccgtct
       61 ggcgacaaac ggcggcaccc cgagttcccg ttcgagtcgt gacggatcct ccccggcttc
      121 cagcctgacc agtgccttgt gagcccggcg gatcgaggtc gaagtcgaga agacggtgct
      181 gcccgcactc acaccctggg cgagcaggcg ctcggcgacg gcgatcactt cccgccggtc
      241 cccgctgatt accgcatcgc cgagagcgaa gttgctggtt tcggatgtgt cggtgaccat
      301 ctcttcgagg tcctcgacgt cgaccagccc gtcggggccg gcccagagcg ccagccggtc
      361 aagttcgttc ccgagccggg gcaggctctc ccccatccgg gcgatcatga agcgggcggc
      421 ctccggggtc agctcgaatc cccgggccgc tgcactctcg atcaggtgca ccggcagccg
      481 ggcgccttcc ggagcttcat aggaaagatc ctcgcccccg accttcttca cggcttccgc
      541 gatcccggcc ggaaccttgc cccgcgcgat aagtaccacc gtcgtctcgt cgggcgcggc
      601 cagcagcgcc tcggcgaccg tcgcggactg gttcttgccc catttctcga tccggtcggc
      661 gagcaggtat cgccggcccg gcatcagcga gagcgaaccg atcgaggcgg caagcgcatc
      721 ggcgtcgggg cttcccttgc cgtcggccgg ttcgaagacc tccagaccac cggcaccgct
      781 ctcggactcg gcccgttgcc gcaaccgggt gcgggcctgg tcgatcttgg cgctgtcgct
      841 gccatggatg aggtaggcgg gcttcacccc ttcattgtcc cggatgaccc ggcgttcaac
      901 cctcaccgcc ccgtttccac ccggtagccc gggccggcgc cgagtacgat cgacacggtg
      961 ccgtcccggt cggtgcgcag ggtctcgacg ccgtccgcgg aaagttcact cagcgcgtcc
     1021 gcggccgggt ggccgtagcg gttgtcctcg ccgaccgaga tcacggcgag ccggggcgag
     1081 gattcatcga ggagcgccgg cagtcccgca tcctcgctg
//
LOCUS       DAOQMF010000740         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_121039_length_1117_cov_3.077213, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000740 DAOQMF010000000
VERSION     DAOQMF010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121039_length_1117_cov_3.077213"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLV77_10710"
     CDS             complement(<1..303)
                     /locus_tag="PLV77_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16363.1"
                     /translation="MKNQKRVEELRELIDQESEGPVLSVFCRTDPRDPANSSETPAWA
                     IALKNGLNHVAGFHEGNHGKEQTVKKLCAEAERRINGASAADRGRSVALFIGGDGKV"
     gene            complement(415..>1117)
                     /locus_tag="PLV77_10715"
     CDS             complement(415..>1117)
                     /locus_tag="PLV77_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16364.1"
                     /translation="PIDNRPFPRFVADASRHGIPHGRFAERLATAFKDACREIDDLPA
                     GTEIPEEFTWFPERAWSGRVWVPVSADGEAVLEENSEPEPIEFFGFVSFVQPEGGDPT
                     DFKASADFTDVVAADNPDWTIDISDEVIGTWLGEQGREAAMTLVWGRALVRDAFAVTA
                     ELDDVTVDQDPLFGGQGGRADRFTLIAPDALKNYGDDAYLEIKLWTSRGKQVARESLY
                     EIEEPQTAPEQEPAE"
BASE COUNT          186 a          359 c          361 g          211 t
ORIGIN      
        1 gaccttcccg tcaccgccga tgaagagcgc gacgctgcgc ccgcggtcgg cggccgaggc
       61 cccgttgatc cgccgttcgg cttcggcaca gagcttcttc accgtctgct ccttgccgtg
      121 gtttccctcg tgaaaacccg ccacgtggtt aaggccgttc ttcaaggcga tcgcccaggc
      181 cggggtctcg ctgctgttgg ccgggtcccg agggtcggtc cggcagaaga ccgaaagcac
      241 cggcccctcg gattcctggt cgatcagttc ccgaagctcc tcaacccgct tttggttctt
      301 catggaacca aaatacctgc atgcattccg gtcttcgccg gaatgccagt aaaacccgcc
      361 cggtcacgac atccacaaat ggatgaccgg gctccatcgg tggctattcg gtggctattc
      421 ggcgggttcc tgttccggtg ctgtctgcgg ttcttcgatt tcgtagaggg attcccgtgc
      481 gacctgtttg ccgcgggagg tccagagctt gatctcgagg taggcgtcat cgccgtagtt
      541 cttgagcgcg tcgggagcga tcagggtaaa gcggtcggcg cgaccgccct ggccgccgaa
      601 aaggggatcc tggtcaacgg tgacatcgtc gagctcggca gtcaccgcga aggcgtcacg
      661 gaccagggcc cggccccaga ccagggtcat cgccgcttcg cggccctgct ctccaagcca
      721 ggtgccgatc acttcatcgg agatgtcgat cgtccagtcg gggttgtcgg cggcgaccac
      781 gtcggtgaag tcggccgagg ccttgaagtc ggtcggatcg ccgccctccg gctgaacgaa
      841 gctgacgaag ccgaagaact cgatcggctc gggttcgctg ttttcctcga ggaccgcttc
      901 gccgtcggcg gaaaccggaa cccagacccg gcccgaccag gcccgctccg gaaaccaggt
      961 gaactcttcc gggatctccg ttccggccgg caggtcatcg atctcgcggc aggcgtcctt
     1021 gaaagcggta gccagccgct cggcaaagcg gccgtgagga atgccgtgac gggaagcatc
     1081 ggcaacgaag cgggggaatg ggcgattgtc gatcggc
//
LOCUS       DAOQMF010000741         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_121268_length_1116_cov_3.162111, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000741 DAOQMF010000000
VERSION     DAOQMF010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121268_length_1116_cov_3.162111"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLV77_10720"
     CDS             complement(<1..321)
                     /locus_tag="PLV77_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16365.1"
                     /translation="MNKAIAVFQAVATCCVMAQADWLLNPYETVAADSAEVNAIYGIQ
                     AFANPASEVSITVPAAGVIQLAGTKIASDGTEGYTANIGLLHPLTPDWAITDVTGVTA
                     ITLDV"
     gene            complement(731..>1116)
                     /locus_tag="PLV77_10725"
     CDS             complement(731..>1116)
                     /locus_tag="PLV77_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNC16366.1"
                     /translation="FNPAKLKQLAWEVKIDDQKSSAIVKDTAAFTVTDVKFYGISDLP
                     VEPGTGVKGKVAKSAFGASYKSGELSLQGLAGFKSVDVVSISGKTVASVSPVGKTTVK
                     LDRGTYVLVAKGEGKTLTRKLAVAQ"
BASE COUNT          245 a          334 c          319 g          218 t
ORIGIN      
        1 cacatccagg gtgatggccg tgacgccagt gacgtcggta atggcccaat ccggagtcag
       61 agggtgcaac aggccgatat tggccgtgta accctcggtg ccgtcggaag cgatcttggt
      121 gccggccagc tgaatcacgc cggcagcggg cacagtgatc gaaacctcag aagccggatt
      181 tgcaaaggcc tggatgccgt aaatggcatt gacctcggcc gagtcggccg caaccgtctc
      241 gtagggattc aacagccagt ccgcttgggc catcacgcaa caggttgcca cagcctgaaa
      301 aacggcaatc gccttgttca tgggagaact cctgtctttg gggagacctg agggaaactc
      361 gatcaggttc acaagttagg tcgctgcgca tcaaacatgg gttcaaccta gatcccgcaa
      421 taggaccggg cgcgctgcca gccatctcgt tcgctcctgg gcttttcgcc tccttccccc
      481 gcaccatcca ggtgcgccgt cagtcggacg aaaaccccat tcatcaaacg atctggcagc
      541 catcacatcc ctctctatca cgggatctag gttcaatcct tgtgggcaca aaacagatca
      601 ggggtatcca taaatctctc cctgtcattt ctgtcgcagg atacctgttc ggtttgaagg
      661 cggcaagaaa caagaagccc accccggcag atgccgaggt gggccaaaga tcagatctga
      721 ggatccgaat ttactgagcc acagccagct tgcgggtcag ggtcttgccc tcgcccttgg
      781 ccacgagcac gtaggtgccg cggtcgagct tgacggtggt cttgccaacc ggcgagacgg
      841 aagcgacggt cttgcccgag atggacacca cgtcgaccga cttgaagcca gccaagccct
      901 ggagcgaaag ctcgccggac ttgtaggaag ctccgaacgc gctcttggca accttgccct
      961 tgacgccagt tcccggctca accggaaggt cagaaatgcc atagaacttg acatccgtga
     1021 cggtgaacgc ggcggtgtcc ttgacgatgg ccgaagactt ctggtcgtcg atcttcactt
     1081 cccaagccag ctgcttcagc ttggcagggt tgaagg
//
LOCUS       DAOQMF010000742         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_121745_length_1114_cov_2.576015, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000742 DAOQMF010000000
VERSION     DAOQMF010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121745_length_1114_cov_2.576015"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..502)
                     /locus_tag="PLV77_10730"
     CDS             complement(269..502)
                     /locus_tag="PLV77_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16367.1"
                     /translation="MAVQPQQTSWTDERLDDLNQSIRDGFARNDREHQEFRQEMREMR
                     TELNDQFRTLHRVMIAGLFTLCAAIIAAGFLPY"
     gene            complement(576..>1114)
                     /locus_tag="PLV77_10735"
     CDS             complement(576..>1114)
                     /locus_tag="PLV77_10735"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HNC16368.1"
                     /translation="IPDRLNFGGQFRVGQREPNTGLTMVLDGYSAAPGEVSGRIDTPT
                     GGLVLRDDFGNVAAMWPYPELISHWNRKHARAAYVPAISRIENGERQFKFDRQVFLGV
                     QTDFLRFLGSMNLGQIVYDPGIKVEGNSTSTPITKRRSQFRTKFEHLSLLYAAFEIQP
                     VSLASAADVESPSFSSFG"
BASE COUNT          250 a          320 c          311 g          233 t
ORIGIN      
        1 caccgggctc aagttcggag accgggcaga cgggaatctt tgtggtttct gtgctcataa
       61 ggtgattggg agggggtccg gcaagccgct cggcagaagg ccagctgcgg ataaacccgg
      121 acaataagcc gacaatgcgg ctaggaatta gatcttaccg actcaacccc accggcaccc
      181 cccattaggc acccctaacg cactcgccaa gcggttttcg cggccagctt catccggtgc
      241 gccagcgcac ccaccgcacc tcaagcaatc agtaaggcag aaatccagcc gcaatgatcg
      301 ctgcgcacag ggtgaacagt ccggcgatca tcacccgatg gagagtgcga aattgatcgt
      361 tcaattcggt tcgcatctct cgcatttcct ggcgaaactc ctgatgttcc cgatcattgc
      421 gcgcaaaccc atcccgaatc gactgattta agtcgtcgag tcgctcgtcg gtccaagaag
      481 tctgttgcgg ctgtacggcc atgaatccag actactccca ggaggccggg agacctcgtg
      541 tctttcgagc gccgccgaac gccggagtgg ctggttcaac cgaaactgga gaatgacggc
      601 gattcgacgt cagcggctga cgccaacgaa actggctgga tctcaaatgc ggcgtagaga
      661 agcgaaaggt gctcgaattt cgttcgaaac tgactgcggc gctttgtgat cggagtcgaa
      721 gtgctgttcc cttctacttt gattcccggg tcataaacga tttgacccag attcattgag
      781 ccgagaaatc tcaggaaatc ggtttgaact ccgagaaaaa cctggcggtc gaatttgaat
      841 tggcgttcgc cgttctcgat ccttgagatt gcgggaacgt aggcggctcg ggcatgtttg
      901 cggttccaat ggctgatcag ctccgggtag ggccacatag ccgccacgtt cccgaaatca
      961 tctctcagca cgagacctcc ggtcggagta tcgatacgac cgctaacttc gcctggcgcc
     1021 gccgagtagc catcaaggac catcgtcaag ccagtattgg gttctctctg tccgactcgg
     1081 aactggcctc cgaagttgag acgatccgga atgc
//
LOCUS       DAOQMF010000743         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_121911_length_1113_cov_3.495274, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000743 DAOQMF010000000
VERSION     DAOQMF010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121911_length_1113_cov_3.495274"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1113
                     /locus_tag="PLV77_10740"
     CDS             13..>1113
                     /locus_tag="PLV77_10740"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNC16369.1"
                     /translation="MRLIITEKNNSAQKIAEILSHGAATETKSFTVPVFRWEDSDGET
                     AVIGTGGHFVGREFPQEKEYKQWKLDLIPGLIDAPLETGPIDGKKNVIKAVQKEAKKA
                     DSLVIGTDFDREGELIGLEALEVCLEVNPDLEHTLERARYSALTREEIEGAFANLDQL
                     SYPLANAAGARQDIDLIWGAAFTRAVSLVAKAYGANFLSVGRVQSPTLGLIVERELER
                     RAHVAKPFWELFARFEHPSGHSFEAHHATDKFWDKGEADAALSGTASPGTVKSVTSRK
                     SSSKPPTPYNTNSFQVDASSRLGITPKRAMDLAQDLYDDGFISYPRTDNTIYPDSLPL
                     EKTIRSLVKIKDFAAAAPILEKPLTPTQGKKFD"
BASE COUNT          228 a          386 c          340 g          159 t
ORIGIN      
        1 tctcgccagc caatgcgact catcatcacc gagaaaaaca actcagccca gaagatcgcg
       61 gagatcctct cgcacggcgc tgcgaccgaa accaagtcgt tcaccgtgcc cgtgttccgc
      121 tgggaagatt cggacggaga aaccgccgtg atcggtaccg gtgggcactt cgtcgggcgg
      181 gagttcccgc aggagaagga atacaagcag tggaagctcg acctgatccc gggcctgatc
      241 gacgcgccgc tggagacggg cccgatcgac ggcaagaaga acgtgatcaa ggccgtccag
      301 aaggaagcca agaaggccga cagcctggtg atcggcaccg acttcgaccg cgagggcgag
      361 ctgatcggcc tcgaggcgct cgaagtctgc ctcgaggtga accccgatct cgagcacaca
      421 cttgaaaggg cgcgttactc ggccctgacc cgggaagaga tagagggcgc cttcgcaaac
      481 ctcgaccagc tctcctaccc gcttgccaac gcggccggcg cccgccagga catcgacctg
      541 atctggggtg ccgccttcac ccgcgccgtc tcgctggtcg ccaaggccta cggggccaac
      601 ttcctttcgg tcggccgggt gcagagcccg accctcggcc tgatcgtcga gcgggagctg
      661 gagcggcggg cgcacgtcgc caagccgttc tgggagctct tcgccaggtt cgagcatccg
      721 agcggccaca gtttcgaggc ccatcacgcg accgacaagt tctgggacaa gggcgaggct
      781 gacgcggcgc tctccggcac cgccagtccg ggcacggtca agtcggtgac cagccgcaag
      841 tcctcgagca agccgccgac cccctacaac acgaactcgt tccaggtcga cgcgtcgagc
      901 cgcctcggga tcaccccgaa gcgggcgatg gacctcgccc aggacctcta cgacgacggt
      961 ttcatctcct acccgcgaac cgacaacacg atctacccgg attcgctgcc gctggagaag
     1021 acgatccgct cgctggtcaa gatcaaggac ttcgccgccg cggccccgat cctcgaaaag
     1081 ccgctcacac ccacccaggg caagaagttc gac
//
LOCUS       DAOQMF010000744         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_121958_length_1113_cov_2.780718, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000744 DAOQMF010000000
VERSION     DAOQMF010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121958_length_1113_cov_2.780718"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLV77_10745"
     CDS             <1..520
                     /locus_tag="PLV77_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16370.1"
                     /translation="ATWLGESGGLAGQPVAGRAGRVGQEMTVKGFDQMRNPTNTPSRI
                     RKALQVSGFMAPRGTIVDVFGGPVKGMTGPLELAMCLMALSASGQIQPIDWKTLIVGQ
                     VDWKGNITAGSDTLAYAIYQRLKERSDYQLICPARMLEQMPVNHQFTHKPIGLGHLSE
                     IRSHPVFTERLG"
     gene            751..942
                     /locus_tag="PLV77_10750"
     CDS             751..942
                     /locus_tag="PLV77_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16371.1"
                     /translation="MALTTDSTSITHPGPNKWAMAMDQIDDHRLADTAVTELRFCEHS
                     GEYASTDAPRPEPEVIWAD"
BASE COUNT          238 a          388 c          333 g          154 t
ORIGIN      
        1 ggccacctgg ctgggcgaaa gcggcggcct tgccggacag ccggtcgccg gccgggccgg
       61 acgggtcggc caggaaatga cggtcaaagg gttcgatcag atgcgaaacc ccaccaacac
      121 gccgtccagg atccggaagg cccttcaggt ttccgggttc atggcaccgc gcggcacgat
      181 cgttgacgtg ttcggcggcc cggtgaaagg aatgaccgga ccgttggagc tcgcgatgtg
      241 cctgatggca ttgtcggcgt ccgggcaaat ccagccgatc gactggaaaa ccctgatcgt
      301 cggccaggtc gactggaaag gcaacatcac cgccggcagc gacacgctcg cctacgcgat
      361 ctaccagcgc ctgaaagagc gttccgacta ccagctgatc tgtcctgccc ggatgctcga
      421 gcagatgccg gtcaaccacc agttcaccca caagccgatc gggcttgggc atctgtccga
      481 gatccgctcg cacccggtgt tcaccgaacg actcggctga acccggtccg catcacggcc
      541 cgtcagcagc caccaggcgg ctgccctgcc cctcccggga caggttccgg ttcgagtccg
      601 gacgggccct ttcacaagcg cacgtggctt gtgaacggtt cagcgaccgc ttcgctgacc
      661 caaaagaaaa cccccgcctt ccttccagag acgcgggggc tttcggaaac ccaaatccgg
      721 ccgaaaggcc agaaagagag tgagccaatt atggcactca ccaccgacag caccagcatc
      781 acccatcccg gcccgaacaa gtgggccatg gcgatggacc agatcgacga tcaccgtctc
      841 gccgacaccg cggtcaccga actgcggttt tgcgagcact ccggcgagta cgcgtccacc
      901 gatgcgcccc ggccggaacc cgaagtgatc tgggccgact agcccggccc caggaagcac
      961 ccagggcatg tcgcgcgatc tagaggcgcg gcatccccaa ccgcagtaca cggccgtcgg
     1021 gaccgcttcc cggcacccac gcagcaccag acccccgagc ccgggggaga aataaggaga
     1081 tccaaaaatg gataccgcaa ccgcaaacag cag
//
LOCUS       DAOQMF010000745         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_122387_length_1111_cov_2.840909, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000745 DAOQMF010000000
VERSION     DAOQMF010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122387_length_1111_cov_2.840909"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1111
                     /locus_tag="PLV77_10755"
     CDS             29..>1111
                     /locus_tag="PLV77_10755"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC16372.1"
                     /translation="MTGNDLPANLPRPRSAAVFDGPDRAAARAYMKGIGLSDEDLSKP
                     TIGIANTWTEAMPCNYGLRDLAAYVKEGVKAAGGTPMEFNTVAVSDGITMGTQGMKSS
                     LVSREMVADSIELMARGYQFDAIIALAACDKTIPGAVMGVARIDIPSIVLYGGSVKPG
                     RYKGKDVSILEVFEAVGAHIAGRITDEELTEIENVASPGYGACGGQFTANTMACAFEA
                     LGISPAFSAMVPAEDEQKTVVAEKVGELILQVLADDLKPSRIITRKSLENAIAAVCAS
                     GGSTNGVLHLLAIAREAGVELDIDVFEEVSRRTPLLADLKPGGRYVATDLYRAGGVPL
                     ILRNLKQGGLLHEDEITVTGRSIGEEV"
BASE COUNT          207 a          359 c          379 g          166 t
ORIGIN      
        1 aggtggaagg cggctctagg atgggaacat gaccgggaac gacctgcctg cgaatctgcc
       61 ccgtcctcga tcggctgcgg tcttcgatgg tcccgaccgg gcggccgccc gggcctacat
      121 gaagggcatc ggactgagtg acgaagacct cagcaagccg accatcggaa tcgccaacac
      181 atggaccgag gcgatgccct gcaactacgg cctgagggac ctcgcggcct atgtcaaaga
      241 aggtgtgaag gcggccgggg gcaccccgat ggagttcaac acggtcgccg tctccgacgg
      301 catcacgatg ggcacccagg gaatgaagag ttccctggtc agtcgcgaga tggtcgccga
      361 ctcgatcgag ctgatggccc gcggctacca gttcgacgcg atcatcgcgc tcgccgcctg
      421 cgacaagacg atccccggcg ccgtgatggg tgtggcccgg atcgacatcc cctcgatcgt
      481 cctctacggc ggctcggtca agcccggcag gtacaagggc aaggacgtca gcatcctcga
      541 ggtcttcgag gcggtcggtg cccacatcgc cggcaggatc accgacgagg aactgaccga
      601 gatcgagaac gtggccagcc ccggttacgg cgcctgcggc ggccagttca cggccaacac
      661 gatggcctgc gccttcgagg cactggggat ctcgccggcc ttctcggcga tggtcccggc
      721 cgaggacgaa cagaagaccg tggtcgccga aaaagtcggg gagctgatcc tgcaggtcct
      781 ggccgatgat ctgaagccga gccggatcat cacgcgcaag tcgctggaaa acgcgatcgc
      841 cgcggtctgc gcttccggcg ggtcgaccaa cggggttctt catctgctgg cgatcgcccg
      901 tgaagccggg gtggagctgg acatcgacgt cttcgaggag gtctcccgcc gcacccccct
      961 gctggccgac ctgaagcccg gcgggcggta cgtcgcgacc gacctctacc gggccggcgg
     1021 ggtgccgctg atcctcagaa acctcaagca gggcggcctg ctgcatgaag acgagatcac
     1081 ggtgaccggg cgcagcatcg gcgaggaggt c
//
LOCUS       DAOQMF010000746         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_122604_length_1110_cov_2.897630, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000746 DAOQMF010000000
VERSION     DAOQMF010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122604_length_1110_cov_2.897630"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="PLV77_10760"
     CDS             complement(<1..108)
                     /locus_tag="PLV77_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNC16373.1"
                     /translation="MRVLVIDNYDSFTYNLVQYLGELGAEVTTVRNDVRP"
     gene            complement(108..>1110)
                     /locus_tag="PLV77_10765"
     CDS             complement(108..>1110)
                     /locus_tag="PLV77_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNC16374.1"
                     /translation="HLHNELTIISCAWADEHENFDQAWEAAAERIGAVRESLRGPVPD
                     AGETGAVEAPEFTSNVTRDEFEATVARIVEYIHAGDAFQVVPSQRFSADQKAEALSIY
                     RGLRSINPSPYMYFFEFGDFQIAGASPEPLLKVNGDRVEIRPIAGTSPRGEDEEEDRR
                     LAEKMISDPKERSEHVMLVDLARNDIGREAGYGTVEVEELMVVETYSHVMHIVSRVSG
                     RLPEGRGALDVLRSALPAGTLSGAPKVRAMQIIDEMEKVKRCSYGGAVGYLSWNGDLD
                     TAIHIRTALIKDGQVHIQAGGGTVADAKPEYEYEESVNKAKAMFRAVEIACENPDWA"
BASE COUNT          172 a          361 c          361 g          216 t
ORIGIN      
        1 cgggcgaacg tcgtttcgaa ccgtggttac ctcggcgccc agttcgccca ggtactggac
       61 caggttgtag gtgaaggagt cgtagttgtc gatgacgagc acccgcatca ggcccagtcc
      121 gggttttcgc aggcgatctc gaccgcgcgg aacatcgcct tcgccttgtt gaccgactct
      181 tcgtactcgt actccggctt cgcgtcggcg accgtgccgc ccccggcctg aatgtgaacc
      241 tggccgtcct tgatcagggc ggtgcgaatg tggatcgcgg tgtcgaggtc accgttccag
      301 gagaggtagc cgacggcgcc gccgtaggag cagcgtttga ccttctccat ctcgtcgatg
      361 atctgcatcg cccggacctt cggcgcgccg gaaagagtgc cggcggggag ggcggaacgg
      421 agcacgtcga gggcgccgcg gccctccggc agcctgcccg agacccggct gacgatgtgc
      481 atcacatgcg aataggtctc gaccaccatc agctcttcca cctcgaccgt gccgtacccg
      541 gcttcgcggc cgatgtcgtt tcgggcgagg tcaaccagca tcacgtgctc ggatcgctcc
      601 ttcgggtcgg agatcatctt ctcggcgagg cggcgatcct cttcctcgtc ctccccgcgc
      661 ggcgaggtgc cggcgatcgg ccggatctcg acgcggtctc cgttcacctt gagcaaaggc
      721 tcgggtgagg ctccggcgat ctggaaatca ccgaactcga agaagtacat gtacggcgag
      781 gggttgatcg atcgaagccc ccggtatatc gagagggctt cggccttctg atcggcgctg
      841 aacctctgtg agggcaccac ctggaaggcg tcgccggcgt ggatgtactc gacgatccgg
      901 gcgaccgttg cctcgaactc gtcccgcgtc acgttggaag tgaactcggg agcttcaacc
      961 gcgccggtct cgcccgcgtc gggcaccggt ccgcgcaggc tttccctgac cgccccgatc
     1021 cgttcggcgg ctgcttccca ggcctggtcg aagttctcat gctcgtcggc ccaggcgcaa
     1081 ctgatgatgg tcaactcgtt gtggagatgg
//
LOCUS       DAOQMF010000747         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_122837_length_1109_cov_3.472486, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000747 DAOQMF010000000
VERSION     DAOQMF010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122837_length_1109_cov_3.472486"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLV77_10770"
     CDS             complement(<1..856)
                     /locus_tag="PLV77_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16375.1"
                     /translation="MSPFRQRIDRLRQGENSPRAPWLVLAGAMALASGFILYYGRDGW
                     FSIDEAQWISDSPQLDLGGALSPHVGHLVFIPRLVYKLMLETVGIEYIAYRILTVLAI
                     LLCTALFFTWARRRLPDFVALAFSLPILLFAYDPLHLIAGNGFTVMLALSFGIAALLA
                     WDREDRTGDIWTFAFLVLGGLTYTVALPFSVAIAFAAVLDRRRDRLWVGAVPIVLYLA
                     WRTLGDVGATDTAAGGGDLLNILLLPAWSFGGISGVLASWSGLSYDFIRSGTMQPGTG
                     IGPALAATL"
     gene            complement(923..>1109)
                     /locus_tag="PLV77_10775"
     CDS             complement(923..>1109)
                     /locus_tag="PLV77_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC16376.1"
                     /translation="VAYIAGFTAPPGKQMGHAGAIISGSSGTAKAKQEALEAKGVRVG
                     ESPTEAARIAVETLRSM"
BASE COUNT          209 a          349 c          401 g          150 t
ORIGIN      
        1 cgagcgtggc ggcgagggcg gggccgatcc cggttcccgg ctgcatggtc ccggagcgaa
       61 tgaagtcata actcaggccg gaccaggaag cgagcacacc ggagatgccg ccgaagctcc
      121 aggccggcag cagcaggatg ttgagcaggt cgcccccgcc cgcggcggtg tcggtggcac
      181 cgacatcacc cagggtccgc caggcgagat agaggacgat cggcaccgct cccacccaga
      241 gccggtcccg gcggcggtcg agaaccgcgg cgaacgcgat cgccaccgag aagggcaggg
      301 cgaccgtgta ggtcaaacct cccagaacca ggaaggcgaa ggtccagatg tccccggttc
      361 ggtcttcgcg gtcccatgcc aggagcgcgg cgatcccgaa ggagagggcg agcatcaccg
      421 tgaaaccgtt gccggcgatc aggtggagcg ggtcataggc gaagagcagg atcggcaggc
      481 tgaaggccag ggcgacgaag tcggggagcc ggcgccgggc ccaggtgaag aagagggccg
      541 tgcagagcag gatcgccagc acggtgagga tgcggtaggc gatgtactcg atgcccaccg
      601 tctccagcat cagcttgtag accagccgcg gaatgaagac cagatgcccg acgtgggggg
      661 aaagcgcccc gccgaggtcg agttgcgggc tgtcggagat ccactgggcc tcgtcgatcg
      721 agaaccagcc gtcacgcccg tagtagagga tgaacccgct cgccagggcc atcgcacccg
      781 cgagcaccaa ccagggcgcg cgggggctgt tctcgccctg tctcaggcga tcgattctct
      841 ggcgaaacgg agacaccggg gtattttcgc gggtttcggc ttccggatgt aaacgccgtg
      901 tgtaaggccc cgccgcggaa aactacatcg atcgcagggt ttcgaccgcg atccgggcgg
      961 cctcggtcgg gctttcgccg acccgcacgc ccttcgcttc gagcgcctcc tgctttgcct
     1021 tggccgtgcc ggaggagccg gagatgatcg cgcccgcgtg acccatctgc ttgccgggag
     1081 gggcggtgaa gccggcgatg taagccacg
//
LOCUS       DAOQMF010000748         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_123577_length_1106_cov_2.561370, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000748 DAOQMF010000000
VERSION     DAOQMF010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123577_length_1106_cov_2.561370"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLV77_10780"
     CDS             <1..642
                     /locus_tag="PLV77_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16377.1"
                     /translation="ISSYAALTGKPKSKKNAAIVLGFGLGLGVVLGSGRLGAGVGAAI
                     IVASATAVAALMMLPGKMTAKRGAILIAAPVIGLALLAVLDLTTAGGQGHYSSVLSGG
                     GDSFLDDIRRRTTLAWQQLWNDTMPVITLVCLLAAAYAIRNRDMFRPWAGPVWPAALV
                     GGLAGGLIGSFTEDSGPLLIVVATITLTGVCSYLLGRPEDAGVASGDIPPDKE"
     gene            645..1013
                     /locus_tag="PLV77_10785"
     CDS             645..1013
                     /locus_tag="PLV77_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC16378.1"
                     /translation="MAVTGTDFVTIPIQDFEASKAFYRDTLGLEEGKQWGNMPAQEFE
                     TGNLTIAIMDPTAFGQDFRPAGGSSVVLQVDDFEASKAELEGKGVKFVTDTIDSGICH
                     QAYFTDPDGNVLGIHHRYAN"
BASE COUNT          203 a          358 c          352 g          193 t
ORIGIN      
        1 atttccagct acgccgccct gaccggcaag ccgaaatcga aaaagaacgc tgcgatcgtg
       61 ctcggtttcg ggctgggact cggtgtggtg ctcggctcgg gccgcctcgg cgccggtgtc
      121 ggagccgcga tcatcgtcgc ctcggcaacc gccgtcgcgg ccctgatgat gctgccggga
      181 aagatgaccg cgaaaagggg ggcgatactg attgccgcgc cggtcatcgg cctggcgctg
      241 ctggccgtgc tcgacctgac tactgccggg ggtcaaggcc actacagctc ggtgctttcc
      301 gggggcggtg acagtttcct cgacgacatc cgccgtcgca ccaccctcgc ctggcagcag
      361 ctctggaacg acaccatgcc ggtgatcacc ctggtctgcc tgctcgccgc cgcctacgcg
      421 atccgcaatc gcgacatgtt cagaccatgg gccggcccgg tctggccggc ggccctggtc
      481 ggcggtctgg ccggcggcct gatcggttcc ttcaccgagg actcgggacc actcctgatc
      541 gtggtcgcca cgatcaccct caccggggtc tgctcgtatc tgcttggacg gccggaggat
      601 gccggggtag catcggggga catcccccca gacaaggagt gaaagtggct gttacaggta
      661 ccgacttcgt gaccattccg atccaggact tcgaggcttc gaaggctttc taccgcgaca
      721 ccctcggcct cgaggagggc aagcagtggg gcaacatgcc ggcccaggaa ttcgagaccg
      781 gcaacctgac gatcgcgatc atggacccga ccgccttcgg ccaggatttc cggccggccg
      841 gcggtagcag cgtcgtcctg caggtcgatg acttcgaagc ctccaaggct gaactggaag
      901 gcaagggcgt caagttcgtc accgacacga tcgacagcgg gatctgccac caggcctact
      961 tcacggaccc ggacggaaac gtcctcggaa tccaccacag atacgcgaac tgagaccgct
     1021 cccggaatca gtgagatccg ggtttttcgt cacataggcg aaaagatcgg atctcgatga
     1081 agagaggacc ttacggcggg ttatac
//
LOCUS       DAOQMF010000749         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_123766_length_1105_cov_2.992381, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000749 DAOQMF010000000
VERSION     DAOQMF010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123766_length_1105_cov_2.992381"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLV77_10790"
     CDS             complement(<1..185)
                     /locus_tag="PLV77_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16379.1"
                     /translation="MNQPTTMLPATAENEPESIYPRFAGLETRRIHGFDAIVANDGPA
                     RQLGFRNLDRKDCPEALL"
     gene            complement(306..578)
                     /locus_tag="PLV77_10795"
     CDS             complement(306..578)
                     /locus_tag="PLV77_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16380.1"
                     /translation="MSLIEEAIRKSEARPVKKVDWDELNRVLPGQKAALTRAAKTGDP
                     EKVAQVTKNAIEVWDRLGAWPDDWSHWQRTLNDLLPWHRQIDIRDL"
     gene            complement(627..1064)
                     /locus_tag="PLV77_10800"
     CDS             complement(627..1064)
                     /locus_tag="PLV77_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16381.1"
                     /translation="MDTATANSSTPAYTTTSDKNTDAFYVSTIGPDRTGIAVSRELIV
                     NCAGDTPTDKALVVLDQVEDGGIIGIEVLNPVHVETAEKDGRFTVELTVGTTDENLVT
                     VEDAIGFDHASWDSYPGVEVDYTVDGRMVAIRLAETDMVEIGL"
BASE COUNT          160 a          320 c          350 g          275 t
ORIGIN      
        1 agtagcgctt ccgggcagtc cttacggtcc aggttccgga agccgagctg acgggcggga
       61 ccgtcattgg cgacgatcgc atcgaatccg tggatccgcc gggtttcaag tccggcgaat
      121 ctcggataga tgctttccgg ctcgttttct gccgttgccg gcagcattgt tgttggctgg
      181 ttcatttctt ctccttgctt cgttttttcc gctgtgggcc gcggacacct tgggcagggg
      241 cctccttgcc ccataaagaa agccccggct tctgccaggg ccctgttacc tctcgagacc
      301 ggctgttaca ggtctcgaat gtcgatctgc cgatgccagg gcagcaggtc attgagggtc
      361 cgctgccagt gcgaccagtc atccggccag gcaccgagcc tgtcccagac ctcgatcgcg
      421 ttcttggtca cctgggcgac cttttcgggg tcgccggttt tcgctgcccg ggtaagggcg
      481 gctttctggc cgggcaaaac ccggttgagc tcgtcccagt cgactttctt gactggccgg
      541 gcctcggatt tgcggatcgc ttcttcgatc agactcatcg aatcgtcaac agtcgataga
      601 gaatcgatag agagtcgatg gatcgtctac aggccgattt cgaccatgtc ggtttcggcg
      661 agccggatcg cgaccatgcg tccgtcgacg gtgtagtcga cttcgacgcc ggggtagctg
      721 tcccagctgg catggtcgaa gccgatcgcg tcttcgacgg tgaccagatt ctcgtcagtg
      781 gtgccaacgg tcagctccac ggtgaagcgg ccatcctttt cagcggtttc gacatgtact
      841 gggttgagca cctcgatgcc gatgatgccg ccgtcttcga cctggtcgag cacaaccagc
      901 gccttgtcgg tcggggtgtc gccggcgcag ttgacgatca gctcgcggct gaccgcgatt
      961 ccggtgcggt ccggtccgat cgttgacacg taaaacgcgt cggtgttctt gtcgctggtg
     1021 gtggtgtagg cgggggtgct gctgtttgcg gttgcggtat ccatttttgg atctccttat
     1081 ttctcccccg ggtgttgggg gtttg
//
LOCUS       DAOQMF010000750         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_124179_length_1103_cov_3.454198, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000750 DAOQMF010000000
VERSION     DAOQMF010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124179_length_1103_cov_3.454198"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..773)
                     /locus_tag="PLV77_10805"
     CDS             complement(15..773)
                     /locus_tag="PLV77_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNC16382.1"
                     /translation="MDLELEAAAATEAAVMVGPMLRRCFENGVVETRSKAVRQDVVTQ
                     ADLDAEQAIIGHLRSKWPEDGFLGEESGSSDGTSARVWCVDPLDGTANFAAGYPRWAV
                     AIALLIRGEAVLAVTFDPLREEIFVAREDVGLTSGPGLSNMRAVARIEDALVADNRPY
                     DHREPAQPLEMEFRRSRGRRESGSMALDFAWLAAGRLDVVSYERSERLWDYIGGELLV
                     AQAGGEIRIADPARDIAVAASPELVEYLCPTKDE"
     gene            complement(764..>1103)
                     /locus_tag="PLV77_10810"
     CDS             complement(764..>1103)
                     /locus_tag="PLV77_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16383.1"
                     /translation="AAVALEFEGDLAGAKACDLLTARFWDAFEEEVRDGWSRRTDRTF
                     PDPFLDRWLVGQYELAIRFAGAEIARRITGVGKANQIDALPEARMLDASRKLLGEARQ
                     LISSGRPAWI"
BASE COUNT          191 a          382 c          345 g          185 t
ORIGIN      
        1 ggctgtccag tcatctactc atccttcgtc gggcacaggt attcgacaag ttcggggctg
       61 gcagcgacgg cgatgtcacg agccgggtcg gcgattcgta tttcgcctcc ggcctgggct
      121 acgaggagct cgccgccgat gtagtcccag agccgctccg agcgctcgta actgaccacg
      181 tcaagccggc ctgccgcgag ccaggcaaag tcgagagcca tcgaaccgga ctcgcgccgg
      241 ccccgcgacc gacggaactc catctccagg ggctgggccg gctcgcggtg atcgtacggc
      301 cggttgtccg cgaccagggc atcctcgatt cgggcgaccg cacgcatatt cgaaagaccc
      361 gggcctgaag tcagaccgac atcttcccgg gcgacgaaga tctcctctcg cagaggatcg
      421 aacgtgacgg ccagcactgc ctcgcctcga atcaagagcg cgatcgcaac ggcccaacgg
      481 ggatagccgg cagcgaagtt ggcggttccg tcgagtggat cgacgcacca cacccgggcc
      541 gatgtgccgt ctgagcttcc gctctcttcg cccaggaagc catcctctgg ccacttgctc
      601 ctcaggtggc cgatgatcgc ctgttcggcg tcgaggtccg cctgggttac cacgtcctga
      661 cgcactgctt tgctccgggt ttccacgacc ccgttctcga aacaccggcg aagcatcgga
      721 ccgaccatga cggccgcttc ggtcgccgcg gcagcttcca gttctagatc catgcgggcc
      781 tcccggacga gatcaactgg cgcgcttcgc cgagcagctt gcggctggca tcgagcatcc
      841 gggcctcagg cagggcatcg atctgattcg ccttgccgac cccggtgatc cttcgggcga
      901 tctcggcccc ggcgaagcga atggccaatt cgtactggcc gacgagccag cgatcgagga
      961 aggggtcggg gaacgtccgg tcggtccgcc ggctccaacc gtcgcgcacc tcttcctcaa
     1021 acgcgtccca gaagcgggcc gtgagcaaat cgcaggcctt tgcgccggca aggtcaccct
     1081 cgaactcgag ggcaacggca gcg
//
LOCUS       DAOQMF010000751         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_124864_length_1100_cov_3.592344, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000751 DAOQMF010000000
VERSION     DAOQMF010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124864_length_1100_cov_3.592344"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLV77_10815"
     CDS             <1..889
                     /locus_tag="PLV77_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16384.1"
                     /translation="TAVLLLSLGLLGAGALGLASTANAARAIDSAPMASDGVRYFAYL
                     TPKGVPAVLDTWTEKTNLIRGAKDCQPLDIGGGRVLLTCGKADKDHMRITRTASARGG
                     MARKLPHNKSGFWFSIGKYWVEGNVPCTGTGGCLGGRAYVNWRTGAIDSPGGIPDDFD
                     RANLDRVKLRYQSPPFVPAGFENSWKVCWNGDVFVTDNGRIRVWKNRHSSMVIGKGGV
                     LKYECDNYQGLGVGSNRVTWNRGPVIHEYNVASGMSYRRRLKKPTRIVPVRDGAVIAM
                     KTRDGRVHDHYRIRFIALP"
     gene            complement(886..>1100)
                     /locus_tag="PLV77_10820"
     CDS             complement(886..>1100)
                     /locus_tag="PLV77_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC16385.1"
                     /translation="DPGTYTWIPFGGGRRRCVGATFAQLEMAIVLRELLRTTEIRAGV
                     DGPEATRRRAITVTPGRGAETILTGR"
BASE COUNT          198 a          353 c          368 g          181 t
ORIGIN      
        1 aaccgcagtc cttcttcttt cgcttggtct gctgggtgcc ggcgcactgg gactggcttc
       61 caccgcgaat gcggcccggg cgatcgattc ggccccgatg gccagtgacg gggtgcgcta
      121 cttcgcctac ctgaccccga agggcgtccc ggcggttctg gacacctgga ccgaaaagac
      181 caacctgatc cggggagcga aggactgcca gccgcttgac atcggcggcg gccgggtgct
      241 gctgacctgc ggcaaggcgg acaaggacca catgcggatc accaggaccg cctcggcccg
      301 cggcggcatg gcaaggaagc tccctcataa caagagcggc ttctggttct cgatcggcaa
      361 gtactgggtg gagggaaacg tgccctgcac cgggaccggt ggctgcctcg gcggtcgtgc
      421 ctacgtgaac tggcggaccg gagcgatcga ctcgcccggt ggcatccccg atgacttcga
      481 ccgggccaac ctggaccggg tgaaactccg ttaccagtcc ccgcctttcg tcccggccgg
      541 tttcgaaaac agctggaaag tctgctggaa cggtgacgtg ttcgtgaccg acaacggccg
      601 gatccgcgtc tggaagaacc ggcactcatc gatggtgatc ggcaagggcg gcgtactcaa
      661 gtacgaatgc gacaactatc agggactagg ggtaggcagc aaccgggtca cctggaaccg
      721 cggcccggtg attcacgaat acaacgtcgc cagcggcatg agttacaggc ggcggctgaa
      781 gaagccgacc aggatcgtcc cggttcgaga cggtgccgtg atcgccatga agacccgtga
      841 cggcagggtt cacgaccact accgcatccg cttcatcgcc ctgccctagc ggccggtcag
      901 gatcgtttcg gctccgcggc ccggggtcac ggtgatcgcc cggcggcggg ttgcttccgg
      961 cccgtcgaca ccggcccgga tctcggtggt gcggagcagt tcgcgcagta cgatcgccat
     1021 ttccagttgc gcgaacgtgg ctccgacgca gcgccgacga ccacccccga agggaatcca
     1081 ggtgtaggtg ccgggatctg
//
LOCUS       DAOQMF010000752         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_125382_length_1098_cov_2.953979, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000752 DAOQMF010000000
VERSION     DAOQMF010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125382_length_1098_cov_2.953979"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1098
                     /locus_tag="PLV77_10825"
     CDS             <1..>1098
                     /locus_tag="PLV77_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC16386.1"
                     /translation="ACVATAAGFGTLIFSSLPLVSQFGLLLGGGVLICFAFTFLAGFA
                     LLGLRGRRRPEPGRGRLIGRVRNLVKSVLATAIMAPGRVLALAVLLGAIGWAVSTQAE
                     VRTEVGQLLPSRAPVVRDLLDVEKTTGASGEIDLIVRAPDVTDPAVVVWTDEVRNTIL
                     NQSGYDTNDPSCEGAELCPGPAIPDFVDPTARGLTSADIRGVLKALPASDRKAMIAGG
                     LTGKGDPTVAKIAFALRAGSVDGQQEVIDRMQSAISDSRGGEGPPPGVSAEVTGLPVV
                     VASSANDLADSRYLLVAAGILAIALVLLLVYRSPRRVLVPLVPIIVAGGWSALIVAAL
                     DLSLNPLSTVLAVLVTAIATEFGVIISGRYFQ"
BASE COUNT          138 a          393 c          375 g          192 t
ORIGIN      
        1 gcctgcgttg cgacggcggc cggcttcggg acgctgatct tctcctccct gccgctggtc
       61 agccagttcg gcctgcttct cggcggcggg gtgctgatct gtttcgcctt caccttcctc
      121 gccggatttg ccctgctggg gctgcgcggc cggcgccgcc ccgaaccggg ccggggccgg
      181 ctcatcggcc gggtgcggaa cctcgtcaag tcggttctgg caaccgcgat catggcaccg
      241 ggcagggtgc tcgccctggc cgtgctgctc ggcgctatcg gctgggcggt cagcacccag
      301 gccgaggtcc ggaccgaggt cggccagctt ctgccttccc gcgccccggt cgtccgggat
      361 ctgctcgacg tggaaaagac aaccggagcc tccggtgaga tcgacctgat cgtccgggcg
      421 cccgacgtga ccgatccggc ggtggtcgtc tggaccgatg aggtcaggaa cacaatcctc
      481 aaccagtccg gttacgacac gaacgaccct tcctgcgaag gggccgaact ctgtcccggg
      541 ccggcgattc cggacttcgt cgacccgacc gcgcgcgggc tgacttcggc cgacatccgg
      601 ggggtcctca aagcgctccc ggccagcgac cgcaaggcga tgattgccgg cggcctgacc
      661 ggcaaagggg atccgaccgt ggcgaagatc gccttcgcgc tccgggccgg ttcggttgac
      721 gggcagcagg aagtgatcga ccgcatgcag agcgcgatct ccgacagtcg cgggggcgag
      781 ggcccgccgc ccggcgtgag cgccgaagtg accggactcc cggtcgtggt ggcctcgtca
      841 gccaacgacc tggccgactc ccgctatctg ctggttgccg ccgggatcct ggccatcgcg
      901 ctggtgctgc tcctggtcta ccgctcgccg aggcgggtcc tggtgccgct ggttccgatc
      961 atcgtcgccg ggggatggtc cgccctgatc gtcgccgccc tcgacctttc gctcaacccg
     1021 ctctccaccg tgctggcggt gctggtcacc gcgatcgcca ccgagttcgg ggtgatcatc
     1081 tccggccgct acttccag
//
LOCUS       DAOQMF010000753         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_127022_length_1091_cov_2.787645, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000753 DAOQMF010000000
VERSION     DAOQMF010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127022_length_1091_cov_2.787645"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLV77_10830"
     CDS             complement(<1..323)
                     /locus_tag="PLV77_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC16387.1"
                     /translation="MKSAVHRHLTVLALLGALLGGLVLAGCGEEGNEPSPGPNYEKAL
                     AGAPPRLAKLYDQANELLPGGLDAFDERVQTDLAGYPAVVNIWASWCGPCRAEFPHFQ
                     QVSAR"
     gene            480..>1091
                     /locus_tag="PLV77_10835"
     CDS             480..>1091
                     /locus_tag="PLV77_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16388.1"
                     /translation="MAPTTAAPAATAAPSKKDIERASEFARESLLLNGDGPDSLIPGT
                     ARRRAFDQAIAELDAGLTEPSVEWRRNFSLLLGLERLIGEEPPTLKDGAELNPHQIDA
                     LSGTLTELLAEQQVSAVDDIETLEVLEEEGDTPLRDESEAEAEPSGEDDEPEDGDEAD
                     EVIDEAVDDDEEPLDWEDKADEDSGDEEEVGEAADDPGAARRFW"
BASE COUNT          214 a          343 c          358 g          176 t
ORIGIN      
        1 atcctggccg agacctgctg gaagtgtgga aactcggccc ggcagggtcc gcaccaggaa
       61 gcccagatgt tgaccaccgc cggatagccg gccaggtcgg tctggacccg ctcgtcgaag
      121 gcgtcgagtc cgcccggcag gagctcgttt gcctggtcgt aaagcttcgc caggcgaggt
      181 ggtgccccgg ccagggcctt ctcgtagttc ggcccggggc tgggctcgtt cccttcttca
      241 ccgcaaccgg ccaggaccag cccgccgagc agcgcgccga gcagggcaag aaccgtcaga
      301 tgtctgtgaa cagccgattt catgcaagta atgattaaat acagacattc gatccgccgt
      361 atcgcggatc acggaggaaa ccggcgtctg tccgtccagc gtcgtcgtcg gaatcttcga
      421 tcacctactc atccgggccg ggtcgagaat ccgggttcgg aacacttgcg aaggctaagt
      481 tggccccgac tactgcagca cccgcagcta ctgcggctcc ctcaaagaaa gacatcgagc
      541 gcgcttctga atttgcgcgt gaatccctgc tgctgaacgg ggacggtccc gattcgctga
      601 ttcccggtac cgcccgccgc cgtgcctttg accaggcgat cgccgaactt gatgccggcc
      661 tgaccgaacc ttcggtcgag tggcgccgga acttctcgct gctgctcggg ctcgaacgcc
      721 tgatcggcga ggaaccgccg accctgaagg acggggccga gctgaacccg catcagatcg
      781 atgccctttc cggcaccctg accgaactgc tggccgagca gcaggtgagt gcggtcgatg
      841 acatcgagac cctggaagtt ctcgaggagg aaggcgacac cccgctccgg gacgagagcg
      901 aagccgaagc cgaaccttcc ggagaggatg acgagcccga agacggggac gaggccgatg
      961 aggtcatcga cgaggccgtg gacgatgacg aggagcccct ggactgggag gacaaggcag
     1021 acgaggattc cggggatgaa gaggaagtcg gcgaagctgc cgacgatccc ggtgccgccc
     1081 gccgtttctg g
//
LOCUS       DAOQMF010000754         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_127040_length_1091_cov_2.638031, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000754 DAOQMF010000000
VERSION     DAOQMF010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127040_length_1091_cov_2.638031"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PLV77_10840"
     CDS             <1..1038
                     /locus_tag="PLV77_10840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC16389.1"
                     /translation="GLDLPGVTVFSIGIIGIVYGLSQAAELGFGSPQVLVPVAIAIVA
                     FIGFWFVEKHSSNPMIEFRLFRHLNFLASNISQFLAGMVELGTAYLLPYFLLMMIGLS
                     PIEAGIALIPATVPIMLAGPLAGRLFDRVGGRIPLVIGFLVLGASGIAFALAVSEREY
                     VYLLPGLILQGIGLGMVLTVNDPTGLDSVPPEDQGQAAGVINTSEQLGGAVGIALFSA
                     VLINTSIHRTNESLAAKGIHPTARQTAEWKEFIQTINSKGLGHIDISKQSETIRFALN
                     DVLSAHVAGYEAVFYSTAGIALLGALACFFLVRKTDRVEQGRVFVRRSRWVSSNTGRT
                     PAVTKHPPRPK"
BASE COUNT          171 a          369 c          334 g          217 t
ORIGIN      
        1 ggcctcgacc tgcccggggt gaccgttttc tcgatcggca tcatcggcat cgtctacgga
       61 ttgagccagg cggccgagct gggtttcggc agcccgcagg tgctggttcc ggtggcgatc
      121 gcgatcgtcg ccttcatcgg tttctggttc gtcgagaagc actcgagcaa tccgatgatc
      181 gagttccggc tcttccggca cctcaacttc ctcgcctcga acatcagcca gttcctggcc
      241 gggatggtcg aactcggcac cgcctacctc ctgccgtact tcctgctgat gatgatcggg
      301 ctgagtccga tcgaagcggg catcgccctg atcccggcga ccgtgccgat catgttggcc
      361 ggacccctcg ccggccggct gttcgaccgg gtcggaggcc gcatcccgct ggtcatcggc
      421 ttcctggtcc ttggcgcttc agggattgcc ttcgccctgg ccgtcagcga gcgcgagtac
      481 gtgtatctgc tccccggtct gatcctgcag ggcatcgggc tcggcatggt cctcacggtg
      541 aacgacccga ccggcctcga ttcggtcccg ccggaagacc aggggcaggc cgccggggtg
      601 atcaacacca gcgagcagct cgggggtgcg gtcggaatcg cattgttctc agcggtcctg
      661 atcaacactt cgattcaccg caccaatgag tcactggccg cgaagggcat ccatcccacc
      721 gcccgtcaga cggcggagtg gaaggagttc atccagacga tcaactccaa gggtctcgga
      781 cacatcgaca tctcaaagca gtccgagacg atcaggttcg ccctcaacga cgtgttgagc
      841 gcccatgtcg ccggttacga ggctgtcttc tactcgaccg ccggcatcgc cctgctgggt
      901 gcgctcgcct gtttcttcct ggtccgcaag accgaccggg tggaacaggg cagggttttc
      961 gtccgccgtt ctcgctgggt cagctcgaac accggccgga ccccggcggt cacaaagcat
     1021 ccgccccggc ccaagtagcc ggctctccgt acgaggtcgc gctcgatcag atcgggctga
     1081 tcgagctttc c
//
LOCUS       DAOQMF010000755         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_127085_length_1091_cov_2.275097, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000755 DAOQMF010000000
VERSION     DAOQMF010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127085_length_1091_cov_2.275097"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLV77_10845"
     CDS             <1..383
                     /locus_tag="PLV77_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC16390.1"
                     /translation="PHWARKERGWSIPKGEIDPGEEPLETARREFREEVGIEPPAGAP
                     IHLGEITQRSGKRVLAWAIEGDFDPTQQTSVMCEIEWPPKSGEKIEIPEVDRVEWVPP
                     ATAKNLVIDGQIPLFDRLEKLVGR"
     gene            402..>1091
                     /locus_tag="PLV77_10850"
     CDS             402..>1091
                     /locus_tag="PLV77_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16391.1"
                     /translation="MKTAAKLLSLGLTVGVLATTATVPASAADTYLDCSAAIDGDGSP
                     AQPRNSLGSAFDLGPGDSLLLKRGTTCHGRLEVTGGGSEIQRARVGAYGSGELPQLNG
                     TGRDAVLLVDTSNLTVGDLDISNPGTGGPIGEGDQIRNGVRVIAQAGTVSDLTIGGLE
                     IHDVDGDLTKTPEGSAAIQISATGAAPVRFTGLHITGNQITSVSRSAISISGSNDLDR
                     PEASQAWPEASS"
BASE COUNT          200 a          365 c          380 g          146 t
ORIGIN      
        1 gtccgcactg ggcccggaaa gagcgcggat ggtcgatccc gaagggggag atcgaccccg
       61 gcgaggaacc gctggaaacg gcccggcgcg agtttcggga agaggttgga atcgagccgc
      121 cggccggggc tccgatccac cttggcgaga tcacccagcg ctccggcaag cgggtgctgg
      181 cctgggcaat cgagggggac ttcgacccga cccagcagac gagcgtgatg tgcgagatcg
      241 agtggccacc gaagtcaggc gagaagatcg agattcccga ggtcgaccgg gtcgagtggg
      301 tgccgccggc caccgcgaag aacctggtca tcgacggcca gatccccctc tttgaccgcc
      361 tcgaaaagct ggtcggccgc tagaacctag acttctcccc gatgaagacg gccgcgaagt
      421 tgctgagcct ggggctgacg gtcggggtgc tcgcaacgac cgcgaccgtc cctgcctcgg
      481 cggccgatac ctacctcgat tgctcggcgg cgattgacgg agacgggtcg ccggcccagc
      541 cacgcaactc gctcggcagt gccttcgacc tcggtcccgg cgacagcctg ctgctcaaac
      601 gcggcacgac ctgccacggc cggctcgagg tcaccggcgg aggcagcgaa atccagcggg
      661 ccagggttgg cgcctacggc tcgggcgagc tcccgcagtt gaacggaacg ggccgcgacg
      721 cggtgctcct cgtggacacc tcgaacctga cggtcgggga tctcgacatc tccaatcccg
      781 gaacaggcgg cccgatcggt gagggcgatc agatccgcaa cggagtgcgg gtgatcgcgc
      841 aggcgggaac cgtcagtgac ctcacgatcg gcggtctgga gattcacgac gtcgacgggg
      901 acctgaccaa gacgccggaa gggagcgccg cgatccagat cagcgcgacc ggcgccgcgc
      961 cggtccggtt cacgggcttg cacatcaccg gcaaccagat cacctcggtg tcgcggagcg
     1021 cgatctcgat ctccggcagc aacgacctcg accggccgga agccagccag gcctggccgg
     1081 aagcttcctc c
//
LOCUS       DAOQMF010000756         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_127128_length_1091_cov_1.852317, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000756 DAOQMF010000000
VERSION     DAOQMF010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127128_length_1091_cov_1.852317"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLV77_10855"
     CDS             <1..794
                     /locus_tag="PLV77_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC16392.1"
                     /translation="PTPGFLSHRAFESLMRWTGQEPPTEEIPIEATVAAMDEAGVGTG
                     MLSAWHAPSGPMIGNDQVAEWCAAHPDRFAGLASVDLARPMEAVRELRRRVEDDGFKG
                     LRQLPWLWETPPTDRRFYPLFAACVELGIPFCTQVGHTGPLMPSEVGRPIPYIDQVAI
                     DFPELVIVGGHIGYPWTEEMVAVCRKHENVYIDTSAYTPKRYPRELIDYMKTKSGRRK
                     VLWGSNFPMIGPAAALEGIGELGLDEETTGLFLGGNARRVFGLPG"
     gene            complement(819..>1091)
                     /locus_tag="PLV77_10860"
     CDS             complement(819..>1091)
                     /locus_tag="PLV77_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16393.1"
                     /translation="HWCDDRLHLNSTGHQVMADGMFSLIEPLPEGQSWLGEITPRQLH
                     GLHERVMAEARWAGAFLAPWIYRRMVGKSSGDGRLAKRPELTPVEV"
BASE COUNT          179 a          388 c          356 g          168 t
ORIGIN      
        1 acccgacccc gggctttctt tcgcaccggg ccttcgaatc actgatgcgc tggaccggcc
       61 aggagccgcc gaccgaagag ataccgatcg aggcgaccgt ggccgcgatg gatgaggccg
      121 gcgtcggcac cgggatgctg agcgcctggc acgcgccatc cgggccgatg atcggtaacg
      181 accaggtcgc tgaatggtgc gcggcccatc ccgaccggtt cgccgggctc gcctcggttg
      241 acctggcccg gccgatggag gccgtgcgtg aactgcggcg ccgggtcgaa gacgatggat
      301 tcaagggcct gcgacagctg ccctggctct gggagacgcc cccgaccgac cgccgcttct
      361 atccgctctt cgccgcctgc gtcgaactag ggattccctt ctgcacccag gtcggccaca
      421 ccgggccgct gatgccttcc gaagtcggcc ggccgattcc ctacatagac caggtcgcga
      481 tcgacttccc cgaactggtc atcgtcggtg gccacatcgg ctacccgtgg accgaggaga
      541 tggtcgccgt ctgccgcaag cacgaaaacg tctacatcga cacctccgcc tacacgccga
      601 agcgctaccc ccgggagctg atcgactaca tgaagacgaa gtccggccgc cggaaggtcc
      661 tctggggatc gaacttcccg atgatcggac cggccgctgc cctggaagga atcggggaac
      721 tcggcctcga cgaagagacc accgggctct tcctcggcgg caacgcccgc cgtgtcttcg
      781 gcctgccggg ctgacctggc gccgggccgc gagaccgctc agacttcgac cggcgtcagt
      841 tcgggccgct tggccaggcg gccatcgccg gaggacttgc cgaccatgcg gcggtagatc
      901 cagggcgcga ggaaggcgcc ggcccagcgc gcctcggcca tcacgcgctc atgcagcccg
      961 tgcagctggc gcggggttat ctcgcccagc caggactgac cctccggcag cggctcgatc
     1021 aggctgaaca ttccgtcggc catcacctga tggcccgtcg agttgagatg cagccggtcg
     1081 tcacaccagt g
//
LOCUS       DAOQMF010000757         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_128457_length_1085_cov_3.461165, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000757 DAOQMF010000000
VERSION     DAOQMF010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128457_length_1085_cov_3.461165"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLV77_10865"
     CDS             complement(<1..357)
                     /locus_tag="PLV77_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNC16394.1"
                     /translation="MKKFHVTTFGCQMNEHDSERMKGMLTSLGYSESTQRDDADLILF
                     NTCSIRENADNRFISHLGEAKRLKKADPERIVGVGGCWAQSVKDEVFRRFPFVDVAFG
                     PGQIHKLAEFLNSDQLS"
     gene            complement(667..942)
                     /locus_tag="PLV77_10870"
     CDS             complement(667..942)
                     /locus_tag="PLV77_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16395.1"
                     /translation="MEIKTVFLIFGISLACFAVIITFIGLRSKNFPSKGALIGLLLLA
                     AFLVGGTTTFAVKLSIEEQHEREEGQKGIGEETTDAAAINIIRQDQA"
BASE COUNT          196 a          338 c          337 g          214 t
ORIGIN      
        1 gctcagctga tccgagttga ggaattcggc cagcttgtgg atctggccgg ggccgaaagc
       61 cacgtcgacg aaggggaatc gccggaagac ctcatccttg accgactggg cccagcagcc
      121 accaactccg acgatccgtt ccggatccgc cttcttcagc cgcttcgcct cgccgaggtg
      181 ggaaatgaaa cggttgtccg cgttctcgcg gatcgagcag gtgttgaaca ggatcagatc
      241 cgcgtcatcg cgctgggtcg attccgagta gccaagcgat gtgagcatgc ccttcatccg
      301 ttcggaatcg tgctcgttca tctggcaccc gaaggtggtg acgtggaact tcttcatggg
      361 cagttcagat tacagtcggc cgtcaagcca cgcccaaccg accccacgac tacatcaacc
      421 cggattcaag gcacggtgcc gcaggttcgc gacctctccg gtaccaaacc tgcgtcaccc
      481 cctgatctga aggcgcggtc agcgggggtt aatcagccgg ggaccggtga ccctcccaaa
      541 gtgccttcgc gattactgct ccgaccatcg agcaggcacg cgttcacgcg acggtagagg
      601 catctttggg agggtctccg gcccccggcg ctgcgcgtcc cgggaagccg agccggtgcg
      661 gaaggcttag gcctggtcct ggcggatgat gttgatggcg gcggcgtcgg tggtttcctc
      721 gccgatgccc ttctggccct cttcacgctc atgctgttct tcgatcgaga gcttgaccgc
      781 gaaggtggtg gtgccaccga cgaggaacgc agccagcagc agaagcccga tcagggcgcc
      841 cttgctgggg aagttcttcg agcgcagccc gatgaaggtg atgatcaccg cgaagcaggc
      901 gaggctgatg ccgaagatga ggaagacggt cttgatttcc atcggcgcga atcctactga
      961 tttcgacggc gttccggcgg catcccgatc agcggtccgg agctccctac cgggcgatcc
     1021 tggtcgatcg gagttccctg atcaccgtga tcttgacctg gcccggatac tccatttccc
     1081 tttcg
//
LOCUS       DAOQMF010000758         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_128894_length_1083_cov_5.140078, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000758 DAOQMF010000000
VERSION     DAOQMF010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128894_length_1083_cov_5.140078"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1008
                     /locus_tag="PLV77_10875"
     CDS             118..1008
                     /locus_tag="PLV77_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC16396.1"
                     /translation="MPAIEIHDLKKSFGPINAVDGLDLVVDEGVCLGLLGPNGAGKST
                     TMKMLTGQAIPDSGVIRVLGYELPGQAKQARAAMGVVPQLDNLDIDVTVEENLAVFAR
                     LYRVDDVAAAVERTLEIARLTDRRKDAVDKLSGGMRRRLLLARGLVHEPKLLLLDEPT
                     VGLDPQIRTELWSLIDNLRSRGTTILMSTHYIEEAERLADEVAVVAGGKVIARATPKE
                     LIAEHAGEKTAEVYGPPERLTEVRSAAEEAGLRVRAAGPAVAIVGAEMAGPGLIPHDA
                     IHRAGSLEDVFVMLTGEDAE"
BASE COUNT          182 a          370 c          369 g          162 t
ORIGIN      
        1 cctcgtgccc cgcccggcct ctgattctgc ccctgatcat ggctcgcagg ctaccgccgg
       61 aaccagacgg ttccggggac cacggggccg ggttcgccgg tctgattgac tgccccgatg
      121 cccgcgatcg aaatccacga cctgaagaag agcttcggcc cgatcaacgc cgtcgatgga
      181 ctcgatctgg tcgtcgatga aggtgtctgc ctgggccttc tcggtccgaa cggcgccggc
      241 aagtcgacca cgatgaagat gcttaccggg caggcaattc ccgattccgg cgtgatccgg
      301 gtactcggct acgaactgcc ggggcaggcc aagcaggcgc gtgcggcgat gggggtcgtg
      361 ccgcagctgg acaatctcga catcgacgtg acggtcgagg agaaccttgc cgtattcgcc
      421 cgcctctacc gggtcgacga tgtcgccgcc gcggtcgagc gaacgctcga gatcgcccgg
      481 ctgaccgatc gtcgcaagga cgccgtcgac aagctctccg gcggcatgcg ccgccggctc
      541 ctgctggcaa ggggcctggt ccacgagccg aaactgctgc tgctcgacga gcccacggtt
      601 ggcctcgacc cccagatccg gaccgagctc tggagcctga tcgacaacct gcgctcacgc
      661 ggcaccacga tcctgatgtc cactcactac atcgaagaag ccgaaaggct ggccgatgag
      721 gtcgcggtgg ttgccggagg caaggtgatc gcccgtgcca ccccgaagga actgatcgcc
      781 gaacatgccg gcgagaagac cgccgaggtc tacggcccgc ccgagcggct gaccgaggtc
      841 cgctcggcag ccgaggaagc cggtctcagg gtccgggcgg ccggtccggc ggtcgcgatc
      901 gtcggagccg agatggccgg accggggctg atcccgcacg acgccatcca ccgggccggc
      961 tcgctcgagg acgtcttcgt gatgctgacc ggcgaggacg ccgaatgaag ccggccggcg
     1021 agacccgctc ggtgcgccgg ctcgaaccgg ccgtgatcct ggccgtgatg ggccgggaat
     1081 tcg
//
LOCUS       DAOQMF010000759         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_129155_length_1082_cov_3.643622, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000759 DAOQMF010000000
VERSION     DAOQMF010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129155_length_1082_cov_3.643622"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLV77_10880"
     CDS             complement(<1..677)
                     /locus_tag="PLV77_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16397.1"
                     /translation="MTGLRRFALAGLCLGLLLAGCGGSDDQATIKPAPDRSARISQLE
                     QRKQNLELQLKAQRKKEAAEAEAKTAEVGGDADANALLTGLPGEAGLVVGAPGGGGPD
                     FSGGSLSSGDAWSTIKVPIAERVLDDFGGPDGISPNQAANIASAITLSDNDAAASLFT
                     DLEGKHGGLEGASAAVGEMLRQAGDRETIISTVGRDGFSTYGQTDWSLENQFRYMAAL
                     VFQRPVGL"
     gene            complement(674..>1082)
                     /locus_tag="PLV77_10885"
     CDS             complement(674..>1082)
                     /locus_tag="PLV77_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16398.1"
                     /translation="LQTQLDAAKAEGAKEARQQARLNQLQGQVKQLQKQSKQSSNGNS
                     GKTGGQGDGYYQDAGSDGGPVSTCATGVQVGPNTSCSFAMNVAGEYGSNPGASTISAY
                     SPVTGEYYTMSCGSWSGGGTVCTGGNGAAVYMP"
BASE COUNT          158 a          383 c          346 g          195 t
ORIGIN      
        1 agaccgactg gtcgctggaa aaccagtgcc gccatgtacc ggaactggtt ttccagcgac
       61 cagtcggtct ggccataggt cgagaaaccg tcccggccca ccgtcgaaat gatggtttcc
      121 cggtcaccgg cctgccggag catttccccg actgccgccg aggccccttc gaggccgccg
      181 tgttttcctt ccaggtcggt aaagagcgag gcggccgcgt cattgtcgga gagggtgatc
      241 gcgctcgcga tgttggccgc ctgattcggg ctgatcccgt ccggcccgcc gaaatcatcg
      301 aggaccctct cggcgatcgg caccttgatc gtcgaccagg catcgccgct ggaaagcgag
      361 cctccactga agtcgggccc gccacccccg ggagcgccga cgaccaagcc cgcctcgccg
      421 ggcaggccgg tcagcagggc gttcgcgtca gcgtccccgc ccacctcggc ggtcttcgcc
      481 tcggcttccg ccgcctcctt ctttcgctgc gccttcagct gaagctccag gttctgtttg
      541 cgctgctcaa gctgcgaaat ccgggcgctg cggtcagggg cgggcttgat cgtcgcctgg
      601 tcatccgatc cgccgcaacc ggccaggagc agaccaaggc agagcccggc cagcgcgaac
      661 cgccgcaggc cggtcaaggc atgtagacgg cagcgccgtt tccaccggtg cagacggtgc
      721 caccgccgct ccaggagccg cagctcatcg tgtagtactc gccggtcacc gggctgtagg
      781 cgctgatcgt agaagcaccg gggttgctgc cgtactcacc ggccacgttc atcgcgaagg
      841 aacaggaagt gttcgggccg acctgcaccc cggtcgcgca ggtcgaaacc gggccaccgt
      901 ccgaaccggc gtcctggtag tagccgtcgc cctggccgcc tgtcttgccg gagttgccgt
      961 tcgagctctg cttcgactgt ttctgcagct gcttgacctg gccctgaagc tggttcagcc
     1021 tggcctgctg gcgggcttcc ttggcgccct cggccttcgc cgcatcgagc tgggtctgca
     1081 gg
//
LOCUS       DAOQMF010000760         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_129399_length_1081_cov_3.810916, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000760 DAOQMF010000000
VERSION     DAOQMF010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129399_length_1081_cov_3.810916"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..>1081
                     /locus_tag="PLV77_10890"
     CDS             219..>1081
                     /locus_tag="PLV77_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC16399.1"
                     /translation="MTDRRLKHWGWGYEDQQPSHEEIVAAAEGIIPMLGFGQAPERPA
                     ELDQVELRQSRLDSPGGIFTDEKHQRTVHALGKAYRDIVRGFYGQFDNPPDLVAFPED
                     EAGVEEVLSRAAGWGAAVIPFGGGTSVCGGVEPRLADPDRPVVSLDMTRMNRLVEVDE
                     TSLAARIQAGARGPELEDQLKAHGLTLRHFPQSFEYATLGGWIVTRAGGHFATGETHV
                     DDLTESIRAITPAGEWQSRRLPGSGAGPSPDRMLLGSEGILGVVTEAWMKVRPRPDFK
                     ATATVEFPPGDA"
BASE COUNT          183 a          381 c          356 g          161 t
ORIGIN      
        1 tgcgatcctc aataccggct acaagctctt cccggattcg aaggcggcca agggcgacaa
       61 ggacggcgag accgaaaagg cgccatcgac cgaagcggtc gccttcgccc acctcctgcg
      121 cggcgtccac tggtaggccc gttgaccgaa tcgagaaggc aaaagtggcc atatagcgct
      181 cccttttgcc atctcgattc ccgtgaccgg gagcagacat gacggatcgc cggctgaagc
      241 actggggctg gggttacgag gaccagcagc cttcccatga ggagatcgtc gccgcggccg
      301 aaggcatcat cccgatgctc ggcttcggcc aggccccgga gcggccggcc gaactcgacc
      361 aggtcgaact gaggcagtcc cggctcgatt cccccggcgg catcttcacc gacgaaaagc
      421 accagcgcac ggtccacgcc ctcggcaagg cctaccgcga catcgtccgc ggcttctacg
      481 gtcagttcga caacccgccc gatcttgtcg ccttcccgga ggatgaagcc ggggtagagg
      541 aggtgctctc ccgggcggcc gggtggggcg ccgcggtgat tcccttcggt ggcggcacct
      601 cggtctgcgg cggggtcgaa ccgcgactgg ccgacccgga ccggccggtg gtctcgctcg
      661 acatgacccg gatgaaccgg ctggtcgagg tcgacgaaac gtcgctcgcc gcccgcatcc
      721 aggccggggc ccgtggcccg gaactggaag accagctcaa ggcacacgga ctgaccttgc
      781 gtcacttccc ccagtccttc gagtacgcga ccctgggcgg ctggatcgtg acccgggccg
      841 gcggccactt cgcgaccggc gaaacccacg tcgatgactt gaccgaatcg atccgcgcga
      901 tcaccccggc cggcgagtgg cagtcccgcc gcctgcccgg ttccggcgcc gggccttccc
      961 cggaccggat gctgctcggc tccgagggca tcctcggagt ggtaaccgag gcgtggatga
     1021 aggttcggcc ccgaccggac ttcaaggcga ccgcgacggt cgagttccct cccggagatg
     1081 c
//
LOCUS       DAOQMF010000761         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_129442_length_1081_cov_2.977583, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000761 DAOQMF010000000
VERSION     DAOQMF010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129442_length_1081_cov_2.977583"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLV77_10895"
     CDS             <1..268
                     /locus_tag="PLV77_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC16400.1"
                     /translation="RHGGDIQLALYRLGAREAWGIEPEAGSYYYVLEGEKVEVQASPD
                     DRERVERTVLEVGEGVLGQDFEPRPSPGVCGWCDFRLVCPAAEA"
     gene            complement(281..>1081)
                     /locus_tag="PLV77_10900"
     CDS             complement(281..>1081)
                     /locus_tag="PLV77_10900"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HNC16401.1"
                     /translation="FRGWNDAASAASTALETLADSLEVDVLAEVDPEDFYDFQANRPT
                     IRLEEGAHRRIEWPGNTFIAARAQGAERDLVLLDGTEPNLKWRTYSEMVVQVAEQLDV
                     QLVVVLGSLIAEVAHTLPVPITGVSTDEQTVRSLELERSDYEGPTGIVGVIHDRCRAA
                     GLPSVSLWAAVPHYVAAVPNPKASLALSEKLESITRVAADISGLEDETMNYEEQIGRA
                     VAADPEVEELVERIEKEQRQRRGPELDVPSGDALAMEFQRFLKNRDSD"
BASE COUNT          164 a          361 c          376 g          180 t
ORIGIN      
        1 ccgccacggc ggtgacatcc agctcgccct ctaccggctc ggggcgcgcg aggcctgggg
       61 gatcgaaccg gaggccggca gctactacta cgtgctcgaa ggcgagaagg tcgaggtcca
      121 ggcctcaccg gatgaccgcg agcgggtcga gcggaccgtg ctcgaggtcg gcgaaggggt
      181 gctcggccag gacttcgaac cgcgcccctc gcccggggtc tgcggctggt gtgacttcag
      241 gctggtctgc ccggccgccg aagcctgagc ccggcaaagg tcagtctgaa tcgcggttct
      301 tgaggaaccg ctggaactcc atcgcaaggg catcgccgct ggggacgtcg agctccggcc
      361 cgcgccgctg gcgctgttcc ttctcgatcc gctcgaccag ttcctcgacc tccgggtcgg
      421 cggcaaccgc ccgcccgatc tgctcctcgt aattcatagt ttcgtcttcg agaccggaga
      481 tgtcggcggc gacccgggtg atcgattcga gcttctcgct gagcgcgagc gacgccttcg
      541 ggttggggac cgccgcaacg taatggggca ccgccgccca gagcgaaacc gagggcaggc
      601 cggcggcccg gcagcggtcg tggatcacgc cgacgatgcc ggtcgggcct tcgtaatcgg
      661 agcgttcgag ctcgaggctg cggacggtct gctcgtcggt tgaaaccccc gttatcggga
      721 ccggcagggt gtgggcaacc tcggcgatca gtgagccgag cacgaccacc agctggacgt
      781 cgagctgctc ggcgacctgg accaccatct cggagtaggt ccgccacttc aggttcggtt
      841 cggtcccgtc gagcaggacc aggtcccgtt cggcaccctg ggcgcgggcg gcgatgaagg
      901 tgttgcccgg ccattcgatt ctccggtggg ctccttcttc caggcggatg gtcggccggt
      961 tcgcctgaaa gtcgtagaaa tcctccgggt ccacctcggc cagcacgtcc acctcgagag
     1021 aatcggccag ggtctccagg gcggtcgagg cggcactcgc cgcgtcattc cagccgcgaa
     1081 a
//
LOCUS       DAOQMF010000762         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_129761_length_1080_cov_2.424390, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000762 DAOQMF010000000
VERSION     DAOQMF010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129761_length_1080_cov_2.424390"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PLV77_10905"
     CDS             complement(<1..852)
                     /locus_tag="PLV77_10905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC16402.1"
                     /translation="MKSVTDSPRQDLGAKAVFVAKAIGRRALWAPYDIKHKLGGGDPL
                     DPPRGLSFVGHGDFRDVGRWYVQQFETLGGLTAEDRILDIGCGIGRMAIPLTDFVKSG
                     SYEGFDTSNAMIRWCQKNITSRNENFRFTHAPIYNRKYNPFGTVLAEDFRFPYDDAQF
                     DFAIATSLFTHLGIVETRHYLEEIARVLKPGGRALVTFFLLGGPGRPADGRDLAFDFA
                     FAFGPLRTTDAREPEAAVAFPEELLDEEVSTAGLGIKPPITYGRWPEVAVGRDIQDMV
                     ILEKTARA"
BASE COUNT          214 a          307 c          328 g          231 t
ORIGIN      
        1 tgcccgagcg gttttctcga ggatgaccat gtcctggatg tcccggccga cggctacctc
       61 gggccagcgg ccataggtga tcggtggttt gatcccgaga ccggccgtgc tcacttcctc
      121 gtcaagcagt tcttcgggaa aggcgacagc tgcttccggt tcccttgcgt cagtggtgcg
      181 aaggggaccg aatgcaaagg caaaatcgaa ggccagatcg cgtccgtcgg ctggccgccc
      241 tggacctcca agcaggaaga aggtgacgag ggcccgcccg cccggtttca gaaccctcgc
      301 gatttcttcg agatagtgcc tcgtttccac gattccgagg tgggtgaaaa gggaagtggc
      361 gattgcgaag tcgaactgcg cgtcgtcata ggggaatctg aagtcttccg ccaggacggt
      421 tccaaaaggg ttgtacttac gattgtaaat cggagcgtgg gtaaagcgga agttctcgtt
      481 gcggctagtg atgttcttct ggcaccaccg gatcatcgcg ttgctggtgt caaaaccctc
      541 ataactgccg gatttcacga agtcggtcag cgggattgcc attcggccaa tgccacatcc
      601 gatgtcaagg atccggtctt ccgctgtcaa gcctccaagc gtctcgaact gctgcacata
      661 ccagcggccg acgtcacgaa agtcaccgtg accgacgaag gagagccccc gtggtgggtc
      721 aagcggatcg cctccgccaa gtttgtgctt gatgtcgtac ggagcccaga gtgctcgcct
      781 accgatggct ttggccacga agacggcttt cgctccaaga tcctggcgtg gtgaatctgt
      841 taccgatttc atctggttcc ctagaatgag ctcagtcggc tttccaggcg atcgcacgta
      901 tcaactcatt acctgaggga tgcgggtcgg acagccagcg tcgctgccta atcgcgagta
      961 gaactcgccg gggggctctc cgcattcgtg acgcgatgta agtgacgaat ggaacggtct
     1021 cagtttcgac ccgactgaag ccggcccttt ccagcaaggt tcgcatcgcc gggggagtcc
//
LOCUS       DAOQMF010000763         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_131688_length_1072_cov_2.717797, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000763 DAOQMF010000000
VERSION     DAOQMF010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131688_length_1072_cov_2.717797"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..654
                     /locus_tag="PLV77_10910"
     CDS             49..654
                     /locus_tag="PLV77_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNC16403.1"
                     /translation="MFELSFISPVSRLAKQSSLLAAFLVAACLLVGVNGQLGAKPADA
                     ASLIAPAKTCPNRAASGGKVAMAKARKSMICMVNYARGREGLRPYRTRQQLTWSSRHK
                     ARDILRCGFSHEACGRSFDFWIKRSGYLGEGGWATGENIAWGSGSLGDVRSIFIAWMK
                     SKGHREAILSRTFTDVGTGVVKGTFQSFHGARIWVLHFGDN"
     gene            784..>1072
                     /locus_tag="PLV77_10915"
     CDS             784..>1072
                     /locus_tag="PLV77_10915"
                     /inference="COORDINATES: protein motif:HMM:NF027503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNC16404.1"
                     /translation="MPEKSELDVLLKTEQTFPPPEGFAAASEFNDPGIYEQADADPEG
                     WWASWAEKLDWIEPWDTILDWSNPPHVTWFAGVMLNVSANCLDRHIEAGRAD"
BASE COUNT          195 a          359 c          339 g          179 t
ORIGIN      
        1 cccgccactc cgcatcactc ctgctgagag gtgttgaatc actagacatt gtttgagctc
       61 tctttcattt cgcctgtttc cagactagcc aagcagtcaa gcctgcttgc cgcttttctg
      121 gtcgcagcct gccttctggt tggcgtcaac ggccagctcg gagccaaacc ggccgatgcc
      181 gccagcctga tcgcgccggc caagacctgc ccgaaccgcg ccgccagcgg tggcaaggtc
      241 gcgatggcca aggcccgcaa gtcgatgatc tgcatggtca actacgcccg gggccgggaa
      301 ggcctccgcc cctaccggac ccggcagcag ttgacctggt catcccgcca caaggccagg
      361 gacatcctgc gctgcggttt ctcgcatgaa gcctgcggaa ggagctttga cttctggatc
      421 aagcggtccg gctatctcgg cgaaggcggc tgggcgaccg gcgagaacat cgcctggggc
      481 agcggatcgc tcggcgacgt gcgctcgata ttcatagcct ggatgaagtc gaaggggcat
      541 cgggaagcga tcctcagccg gaccttcacc gacgtcggga ccggcgtcgt caagggaaca
      601 ttccagtctt tccacggggc ccggatctgg gtcctgcatt tcggcgacaa ctgaccgtcg
      661 cccttcgccc cgttccgggc tgcctcgaag cagtgcccga accgatcctc cggaccggga
      721 gtccggcggg ggctagcatt gggccaccga ggagaaccgg ccccgacccc ggaggagaga
      781 tcagtgccag agaagtcaga gcttgacgtc cttctgaaga ccgagcagac cttcccgccc
      841 cccgaagggt tcgctgcggc gagcgagttc aatgaccctg ggatctacga gcaggccgac
      901 gccgaccccg aaggctggtg ggcgagctgg gcggagaagc tcgactggat cgaaccctgg
      961 gacacgatcc tcgactggag caacccgccc cacgtgacct ggttcgccgg cgtgatgctc
     1021 aacgtcagcg ccaactgcct cgaccgccac atcgaggcgg gccgggcaga tg
//
LOCUS       DAOQMF010000764         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_132501_length_1069_cov_2.299803, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000764 DAOQMF010000000
VERSION     DAOQMF010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132501_length_1069_cov_2.299803"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLV77_10920"
     CDS             <1..425
                     /locus_tag="PLV77_10920"
                     /inference="COORDINATES: protein motif:HMM:NF037020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16405.1"
                     /translation="LTPESYLGSARADRFENGTILPGVHDFGPLKEPKLDWLAYGGKW
                     SISPEDARSDGGQIAVHFRARRVYLVLSSPGKARSAGIELDGKPVSAAAAGDDVKSGQ
                     VEVTSQRLYSLIDLPKPGEHVLTVSPEAGISGYAFTFG"
     gene            491..564
                     /locus_tag="PLV77_10925"
     tRNA            491..564
                     /locus_tag="PLV77_10925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:525..527,aa:Arg,seq:acg)
     gene            complement(719..1033)
                     /locus_tag="PLV77_10930"
     CDS             complement(719..1033)
                     /locus_tag="PLV77_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HNC16406.1"
                     /translation="MTQTKAERSAAAKKAAATRKRNEAKAEAAASDRGKKAAATRQSK
                     AAKQSADQAKRSAGQAKQTAAEAKDSTGSAVGSLANAAKSAGDAALKAGKSVASRVRS
                     GF"
BASE COUNT          158 a          356 c          360 g          195 t
ORIGIN      
        1 tcctgacccc ggagagctat ctcggttcgg cccgggccga ccgcttcgag aacggaacga
       61 tcctgcccgg cgttcacgac ttcgggcccc tgaaggagcc gaagctcgac tggctcgcct
      121 acggcggcaa gtggagcatc agcccggagg acgcacggtc cgacggcggc cagatcgcgg
      181 tccacttccg ggccaggcgg gtctatctgg tcctttcctc accgggcaag gcccgcagcg
      241 ccgggatcga actggacggc aagccggtca gtgccgcggc ggccggagat gacgtgaaaa
      301 gcggtcaggt cgaggtcacg agccagaggc tctacagcct gatcgacctg ccgaaaccgg
      361 gcgagcacgt gctgacggtc agcccggaag cgggcatcag cggctacgcc ttcaccttcg
      421 gctgagccag caggagtccc gaacgctcgc aacctcgtag aatcgccgat ccggggtgtt
      481 tccccaatcc gcgcccgtag ctcagctgga tagagcgttg gtctacgaaa ccaaaggtca
      541 caggttcgaa tcctgtcggg cgcgcttgct ggcggggcag ccttcgcgct accagccgcc
      601 tgttcccggg tcgcctttga tcgggccggt gaagccgcag gttcgtgaac aggttcggac
      661 cctcgttcac ctgggctttg acgccccagg cgttggcgag ggcccgggcc ccggggatct
      721 agaagccgct gcggacgcgg gaagcgaccg acttgccggc cttgagggcg gcgtcaccgg
      781 ccgacttggc cgcgttggcg aggctgccga cggccgatcc ggtcgaatct ttggcctcgg
      841 cggctgtctg cttcgcctgc ccggccgacc gcttggcctg atcggcggac tgcttggctg
      901 ccttgctctg acgggtagcg gcggccttct tgccacggtc gctggcggcc gcttcggcct
      961 tggcttcgtt tcgcttcctt gtggcagctg ccttctttgc ggcggcgctg cgttcagctt
     1021 tggtctgagt catgacactc cggtacccgg accaaaaacc ttcaatcgt
//
LOCUS       DAOQMF010000765         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_132655_length_1068_cov_3.236920, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000765 DAOQMF010000000
VERSION     DAOQMF010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132655_length_1068_cov_3.236920"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>1068
                     /locus_tag="PLV77_10935"
     CDS             112..>1068
                     /locus_tag="PLV77_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC16407.1"
                     /translation="MTDILSRRDLQFQLNEWLDVERLTERDRYRDQTLDDWNDVLTLA
                     EQIAEDQFQPHNRKADLNEPAIGEDGKVILIPEVAQALETLGETGLMAAAMPEEVGGM
                     SLPATVSSAIFAWLHAANVGTTAYPMLTAGAANLLIANASDEQVETWVKPMVEGRFHG
                     TMCLSETQAGSSLSDITTKAVPQDDGTYRIFGNKMWISGGSHELGDNIVHLVLAKIPG
                     SPPGVKGISLFIVPRFLLDEDGSIGGRNDVVLAGVNHKMGFRGTVNTVLGFGEGEYKP
                     GGEAGAVGYLIGEPNRGLAYMFHMMNEARIGVGLGATALGYTG"
BASE COUNT          207 a          350 c          334 g          177 t
ORIGIN      
        1 tctccctcaa aggttggcag tcgatcggat gttccgaccc ggaaactcta ctcatcaacc
       61 cgtcggaggt gccaaaggtg cgatgcatca gaagcgagta gcgttgcgtg aatgaccgac
      121 atcctttccc ggcgcgacct tcagtttcag ctcaacgaat ggctcgacgt cgagcggctg
      181 accgagcgcg accgctaccg cgaccagact ctcgacgact ggaacgacgt gctcacgctt
      241 gccgagcaga tcgccgagga ccagttccag ccccacaatc gcaaggcgga cctgaacgaa
      301 ccggcgatcg gtgaagacgg caaggtcatc ctgatcccgg aggtcgccca ggccctggag
      361 acacttggtg aaaccgggct gatggccgca gcgatgccgg aagaagtggg cggcatgtct
      421 ctgccggcca ccgtttcgag cgcgatcttt gcctggctcc atgccgccaa cgtcggcacc
      481 accgcctacc cgatgctgac cgccggcgcc gccaacctgc tgatcgccaa tgcctccgac
      541 gagcaggttg aaacctgggt caagccgatg gtcgaggggc gctttcacgg caccatgtgc
      601 ctttccgaga cccaggccgg cagctccctc tcggacatca ccaccaaggc ggtcccgcag
      661 gacgacggca cctaccggat cttcgggaac aagatgtgga tcagcggcgg ctcacacgag
      721 ctcggtgaca acatcgtgca tctcgtactg gcgaagatcc ccggttcccc tcccggtgtg
      781 aaggggatca gcctcttcat cgttccgcgg ttcctgctcg acgaagacgg ttcgatcggc
      841 ggacgaaacg acgtggtcct ggccggtgtc aaccacaaga tgggcttccg cgggaccgtc
      901 aacacggtcc tcggcttcgg cgagggcgag tacaaaccgg gcggcgaagc cggcgcggtg
      961 ggctacctga tcggcgagcc gaaccggggt ctcgcctaca tgttccacat gatgaacgag
     1021 gcccggatcg gggtcggtct cggcgcgacg gcgctcggtt acaccggg
//
LOCUS       DAOQMF010000766         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_132927_length_1067_cov_2.650198, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000766 DAOQMF010000000
VERSION     DAOQMF010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132927_length_1067_cov_2.650198"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLV77_10940"
     CDS             complement(<1..433)
                     /locus_tag="PLV77_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNC16408.1"
                     /translation="MDLSGTTVALDGLGVESGFDVLNEGIRLAAGDGIRLRVFGPADA
                     IEVSGLPGVEVIDCTDQITNDDDPVPSVRSRPDSSIVRAVADVGAGNSESVVSLGSTG
                     ATMAASTFGLKRLKGVKRPALAAQVPLPGRTVLFLDIGANVE"
     gene            complement(443..652)
                     /gene="rpmF"
                     /locus_tag="PLV77_10945"
     CDS             complement(443..652)
                     /gene="rpmF"
                     /locus_tag="PLV77_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNC16409.1"
                     /translation="MAVPKKRTSRTRRDKRRATHKAAKARVNHCPHCHHPRLPHRVCA
                     NCGQYKGREVVAPKEQPFGTVTPDA"
     gene            complement(721..>1067)
                     /locus_tag="PLV77_10950"
     CDS             complement(721..>1067)
                     /locus_tag="PLV77_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNC16410.1"
                     /translation="LTRPISRADQMAEVDQPVEPIPIQGSGRIQKDDEDEDESSAAEL
                     TSPYVEEGTLDLAAWTHDALILSLPEQILCQPDCLGLCPVCGESLNGADPAAHDHGQD
                     IDPRWAKLRDLK"
BASE COUNT          169 a          340 c          366 g          192 t
ORIGIN      
        1 cctcgacgtt cgcgccgatg tcgagaaaga gaacggtccg tcccggcagc ggaacctgcg
       61 cggccagggc cggtcgtttg acccccttca gccgtttcag gccgaaggtt gacgccgcca
      121 tcgtcgcccc ggttgatccg aggctgacga ccgattccga attcccggca cctacatcgg
      181 ccaccgcccg gacgatcgag gagtcgggac gcgaccgcac cgaggggacg ggatcgtcgt
      241 cgttggtgat ctggtcggtg cagtcgatca cctcgacccc cggcaaaccg gaaacctcga
      301 tcgcgtcggc cggcccgaaa accctcagcc ggattccgtc tccggcggca agccggattc
      361 cttcgttcag gacatcgaag ccgctttcga cgccgaggcc gtcaagcgcg accgtagtcc
      421 cgctcaggtc caagagttca ggctaggcgt cgggggtgac ggtgccgaag ggctgctctt
      481 tgggagcgac gacctcacgg cccttgtact ggccacagtt cgcgcagacg cgatgcggaa
      541 ggcgcggatg gtggcaatgc gggcagtggt tgacgcgggc cttggcggcc ttgtgggtag
      601 cgcgccgctt gtcccggcgg gtccttgagg ttcgtttctt cggtacggcc ataagacgca
      661 gcagtctaga ggaatggcgg ctgaagtgac atccacccgc ccgggatggc tcccctcctg
      721 ctatttcagg tcgcgcagct tcgcccagcg ggggtcgatg tcctggccat gatcatgcgc
      781 ggcggggtcg gccccgttca gggactctcc gcaaaccggg caaagaccga ggcaatcagg
      841 ctggcagagg atctgctccg gcagggagag gatcagggcg tcgtgggtcc aggcggccag
      901 gtcgagggtg ccttcctcga cataggggct ggtcagctcc gcggcgctgg attcatcttc
      961 atcctcgtcg tctttctgga tccggcctga accctggatc gggatcggtt cgaccggctg
     1021 gtcgacctcg gccatctggt cggcgcgcga gatcggcctc gtgagca
//
LOCUS       DAOQMF010000767         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_132941_length_1067_cov_2.563241, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000767 DAOQMF010000000
VERSION     DAOQMF010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132941_length_1067_cov_2.563241"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLV77_10955"
     CDS             complement(<1..588)
                     /locus_tag="PLV77_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNC16411.1"
                     /translation="MPAAAENLKPPVLILSGGRGTRLREQTDSLPKPLVEVGGKPLIW
                     HVIQIYARQGFTRFFPLTGYLGEQIEEFVEGEVWPEGVSVSCLETGLDTPTGGRIAAA
                     SGQVEGTFCATYADGVADLDLDALLDFHRSSGRVATMTAVRPDLQWGVAEIDPAGEVT
                     GFIEKLRSEHWINGGFFCFEPAVFDWLESDSVLERE"
     gene            complement(572..847)
                     /locus_tag="PLV77_10960"
     CDS             complement(572..847)
                     /locus_tag="PLV77_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HNC16412.1"
                     /translation="MAVTVKIPTQLRATTGGQADLSAEGATVGEVLEAVFAEHGDLRD
                     RITEDGELRRFVNVYVGGEDIRFGDGLGTAVSDGAEVTILPAVAGGC"
     gene            complement(847..>1067)
                     /locus_tag="PLV77_10965"
     CDS             complement(847..>1067)
                     /locus_tag="PLV77_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16413.1"
                     /translation="TGIFTETAGGVTVGVLKKLAERGELKDGEKVVVYITGEGLKTLD
                     ANRDSFALTEIDPNPDSFESHFGQEVVA"
BASE COUNT          184 a          355 c          342 g          186 t
ORIGIN      
        1 ctcgcgctcg aggaccgagt ccgactcgag ccagtcgaag acggcaggtt cgaaacagaa
       61 aaagccgccg ttgatccagt gctcggacct cagcttctcg atgaagccgg tgacctcgcc
      121 ggccggatcg atttccgcca cgccccactg caggtccggc cggaccgcgg tcatggttgc
      181 caccctgccg ctggagcggt gaaagtcgag aagtgcgtcg aggtcgaggt cggcgacacc
      241 gtcggcgtag gtcgcacaga aggttccctc gacctgaccg cttgccgcgg cgattcgacc
      301 gccggtcggg gtgtcgagcc cggtttccag gcaactcacg gaaacgccct ccggccagac
      361 ttcgccttcg acgaactcct cgatctgctc gccgaggtaa ccggtcagcg ggaagaacct
      421 ggtgaatccc tgccgggcgt aaatctggat cacatgccag atcagcggct tgccaccgac
      481 ctcgaccagc ggcttgggca gggaatcggt ctgctcgcgc agccgcgtgc cccggccgcc
      541 gctgaggatc agaaccgggg gcttcaggtt ttcagcagcc gccggcaacg gccggaagga
      601 tcgtcacttc ggcaccatcg gaaaccgcgg tgccgagccc gtcgccgaag cggatgtctt
      661 caccgccgac gtagacgttg acgaaacggc gcagttcgcc gtcttcggtg atccggtctc
      721 ggaggtcacc gtgctcggcg aagaccgctt cgaggacctc gcccaccgtg gcaccctcgg
      781 cgctcaggtc ggcctggcct ccggtggtgg cgcgaagctg ggtcggaatc ttgactgtga
      841 ctgccatcag gcgacgacct cctggccgaa gtgggattcg aacgagtccg gattcggatc
      901 gatctcggtc agcgcaaagc tatcgcgatt ggcgtcgagg gtcttcagtc cctcaccggt
      961 gatgtaaacg acgaccttct cgccgtcttt cagctcaccg cgctcggcca gtttcttcag
     1021 cacgccgacc gtgacgccgc cggcagtctc ggtgaagatg ccggtcg
//
LOCUS       DAOQMF010000768         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_133116_length_1066_cov_3.474777, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000768 DAOQMF010000000
VERSION     DAOQMF010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133116_length_1066_cov_3.474777"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLV77_10970"
     CDS             <1..772
                     /locus_tag="PLV77_10970"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNC16414.1"
                     /translation="GGTLFASGGDTTQSTLRAYTKGKADPETVATGNPGGGGLAWNGK
                     RLLWGYGDLLANGLTGDLDPTAGLYSVNVAAKKKSLVSDTLGMANGVARGKDGYIYAS
                     NNLGLKLDRITPDGDTENGWASLNSANGLTVGRNGRYLYANQMFETPSTIAKIDTRDP
                     TKVFTYFTSPEAGNVLLDGLAHDSGNNLYTAAYGKGEVWKITPDRQACVIATGLSQTT
                     AVAMSSAGKGFRAGSLYAAGFDGNIVEVTGAAKAAVPG"
     gene            complement(814..>1066)
                     /locus_tag="PLV77_10975"
     CDS             complement(814..>1066)
                     /locus_tag="PLV77_10975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC16415.1"
                     /translation="KEPHWYLSVLSVSPESQRQGHGGALLGPGLARADRQGVGCWLET
                     QRESNIPFYARFGFELVKKLEIEAGVNLWLMWRPPLEGD"
BASE COUNT          202 a          368 c          341 g          155 t
ORIGIN      
        1 tggcggaacc cttttcgctt ccggcggtga cacgacccaa agcaccctgc gggcctacac
       61 gaagggtaag gccgatccgg agaccgtggc gaccggcaac ccgggcggcg gcgggctggc
      121 ctggaacggc aagcgcctgc tctggggcta cggagacctt ctggccaacg gactgaccgg
      181 tgacctcgac ccgaccgccg gtctctacag cgtgaacgtc gccgcgaaga agaagagcct
      241 cgtctcggac actctcggga tggcgaacgg agttgcccgc ggcaaggacg gctacatcta
      301 cgcctcgaac aacctcggtc tcaagctcga ccggatcacg cccgacggcg acaccgagaa
      361 cggctgggcc agcctgaact ccgccaacgg cctcacggta ggcaggaatg gtcgctacct
      421 ctacgcgaac cagatgttcg agacaccctc gaccatcgcg aagatcgata cccgcgaccc
      481 cacgaaagtc ttcacttact tcacctcgcc ggaggcaggc aacgtccttc tcgacgggct
      541 ggcgcatgac tccggcaaca acctctacac ggcggcctac ggcaagggcg aggtctggaa
      601 gatcactccg gacaggcagg catgcgtgat cgccaccgga ctttcgcaga ccaccgcggt
      661 agcgatgagt agcgccggca agggcttcag ggccggcagt ctctacgccg ccgggttcga
      721 cggcaatatc gtcgaggtca ccggcgcggc gaaggcggct gtgcccggct gatccgccgc
      781 cacccgggct tcccggcggc agcgccctcg cggtcagtcc ccctcgaggg gaggccgcca
      841 catcagccag aggttcacgc cggcctcgat ttccagcttc ttgaccagct cgaagccgaa
      901 gcgggcgtaa aagggaatgt tgctttcgcg ctgggtttcc agccagcagc cgacaccctg
      961 gcggtccgcc ctggcgagcc cgggcccgag cagcgccccg ccatggcctt gccgctggga
     1021 ctcgggtgag acggaaagca ccgagaggta ccaatggggt tccttt
//
LOCUS       DAOQMF010000769         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_133123_length_1066_cov_3.302671, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000769 DAOQMF010000000
VERSION     DAOQMF010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133123_length_1066_cov_3.302671"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLV77_10980"
     CDS             <1..780
                     /locus_tag="PLV77_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC16416.1"
                     /translation="QAGPHQASEAVVFIHGNPGSAADFTGLIDAVAPPRRAIAFDLPD
                     FGQSIAAPGFGHTKDEYSHFVGDAFKALGIRRAHLVLHDLGGPIGLDWASTHTGQIAS
                     VILINTGVLAGYKWHRTARVWQTPVAGELLQAAAFRSMFRRIITRAEPHGLPRDFVDG
                     MYDNYDRRTRRAVLDIYRDLKKVGKTSKQLIPLLAAADLPCLVVWGAGDPYLPASFAD
                     RQRSAFPRAQVHILPASGHWPFIDDPPAVTELLTAFLATVE"
BASE COUNT          159 a          398 c          328 g          181 t
ORIGIN      
        1 caggccgggc ctcaccaggc ctccgaggcg gtcgtcttca tccacggcaa tccgggttcg
       61 gccgcggact tcaccggact gatcgacgcc gtcgcgccgc cccgccgggc gattgccttc
      121 gacctgccgg acttcggcca atcgatcgcg gcgccgggat tcggtcacac caaggacgag
      181 tactcgcatt tcgtcggcga cgcgttcaag gcgctcggga tccggcgggc ccatctcgtg
      241 ctccacgatc tcggtgggcc gatcgggctc gactgggcgt cgacccacac cgggcagatc
      301 gccagcgtca tcctgatcaa caccggggtc ctggccgggt acaagtggca tcgaaccgcc
      361 cgggtctggc agaccccggt cgccggcgag ctgcttcagg ccgccgcctt ccgctcgatg
      421 tttcggcgga tcatcacgcg ggccgagccg catggcctgc cgcgggactt cgtcgacggg
      481 atgtacgaca actacgaccg tcgcacccgc cgggccgtgc tcgacatcta ccgtgacctg
      541 aagaaggtcg gcaagacctc gaagcagctg attcccctcc tggccgccgc cgacctgccc
      601 tgcctggtcg tctggggcgc gggggatccc tacctgccgg cctccttcgc cgatcgccag
      661 cggagcgcct tcccccgggc ccaggtccac atcctgccgg cctccggcca ctggcccttc
      721 atcgacgacc ctccggccgt caccgagctc ctgaccgcct ttctggccac cgtcgaatag
      781 ccccgccagg cgtcggggtc ttcggccggc gaacccgggt aacggtctca accgggtccg
      841 cagcttttcg ctcccggacg ggttagttga cgtacatcac ttttcgggca agaatggtcc
      901 cgagaaggaa tcgctttgag gagagtgtct tgaaccaccg ccgcatcacc gcaaccgtct
      961 gttgtctcat ggtcgccctg gcgggtgcct tcgccctggc cgctccggca agcgccgcct
     1021 acgacccgaa cgaccccgaa cagaaggccg agtacgacgc tgcatt
//
LOCUS       DAOQMF010000770         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_133680_length_1064_cov_2.784936, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000770 DAOQMF010000000
VERSION     DAOQMF010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133680_length_1064_cov_2.784936"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLV77_10985"
     CDS             <1..490
                     /locus_tag="PLV77_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16417.1"
                     /translation="LPLKAIRALFEEDPAQAEAMLEVEDRILERALAGERTRTSATEV
                     RKYGVPKKVLERLAEIGILTPNSRGYSPSDVEIIEAMSRFRAGGYDEQIGFTVYDTLR
                     YKHALEALVKEEVDVLMERLEGEITPEHAVELIEAGAEPLKDLIAALHTKLMVEEVER
                     RR"
     gene            527..>1064
                     /locus_tag="PLV77_10990"
     CDS             527..>1064
                     /locus_tag="PLV77_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16418.1"
                     /translation="MEIGGVSKGDVLRSLGAAALLLLAALLFAPPAAAALTARDSDPV
                     VLKGSQAGNLAGIDPGRLVAFRWTGSAWDQVPLQVDERKTIDMQSLYPWVTGGANQGY
                     VTNYLIPVNVEVYADPGTRAGADPDATLDPNDEFVFMAFDSGSKAPIDSDPAHVVPRS
                     GVELELDDPCGGGAVELGR"
BASE COUNT          182 a          335 c          370 g          177 t
ORIGIN      
        1 cctgccgctg aaggcgatcc gggcgctctt cgaggaggac ccggcgcagg ccgaggcgat
       61 gctggaggtc gaggaccgga ttctcgagcg cgccctggcc ggcgagcgga cccgaaccag
      121 tgcgaccgaa gtcaggaagt acggcgtgcc gaagaaggtt ctggagcggc tggccgagat
      181 cggcatcctg accccgaact cgcgcggcta ctcgccctcg gacgtggaga tcatcgaggc
      241 gatgtcacgc ttccgggccg gcggctacga cgaacagatc ggcttcacgg tctatgacac
      301 cctgcgttac aagcatgccc tcgaagccct ggtcaaggag gaggtcgacg tgctgatgga
      361 acggctcgaa ggcgagatca cgccggaaca cgcggtcgag ctgatcgaag ccggggccga
      421 acccctcaag gacctgattg ccgccctcca cacgaaattg atggtcgaag aggtcgaacg
      481 ccgccgctga gcggttttgc ccggaaccgg cgttaccctt cccgtgatgg aaattggggg
      541 agtttcaaag ggagatgtgc tgcggtcgct tggggcggcc gctctgctgc tgctcgcagc
      601 gctgcttttc gctccgcctg cggctgcggc gctgaccgcc cgcgattccg atccggtcgt
      661 tttgaaaggc agccaggccg ggaacctggc cgggatcgat ccggggcggc tcgtcgcctt
      721 ccgctggacc gggtctgcct gggaccaggt cccgctgcag gtcgatgaac ggaagacgat
      781 cgacatgcag agtctctatc cgtgggtcac cggcggcgcc aaccagggat acgtgaccaa
      841 ttatctgatc ccggtgaacg tcgaggtgta cgcggacccc ggcacccggg ccggcgccga
      901 ccctgacgcc accctcgacc cgaatgacga gttcgtcttc atggccttcg actcggggtc
      961 gaaggcgccg atcgattccg atccggccca cgtggttccc cgctcggggg tggagctcga
     1021 actcgacgat ccatgcggcg gtggagcggt tgagctgggt cggg
//
LOCUS       DAOQMF010000771         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_133897_length_1063_cov_3.388889, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000771 DAOQMF010000000
VERSION     DAOQMF010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133897_length_1063_cov_3.388889"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1063
                     /locus_tag="PLV77_10995"
     CDS             <1..>1063
                     /locus_tag="PLV77_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC16419.1"
                     /translation="ELGLETFPTWCEGRNVFERGGKARTYSGTIPRLNPVSLAEVGMA
                     LKRLEKMAAKVDVTQPWAQTDLARWDAETFATWARRNAKTPAARDLLRLAIQAVWAAE
                     PEDVSLLHALFYVQSSGGSLEKMLDAEGGAQDSRVVGGTQLIAERMAAELGDAVHLGF
                     PVRRVRWQRMPGDIEDAPVEFGPHPVTVEADELTVSARAAVIALPPAVAGRLEYDPIM
                     PALRDGLTQRMVQGTVVKCHAIYDEPFWRADGLSGQATSADGPVSVTFDNSPPSGTPG
                     VLLAFLEGNAARQATDLPPAERREIVLDCLVRLFGPKAANPERFVDKAWAADEFARGC
                     YGGYLPPGAWLMYGEGLRTP"
BASE COUNT          164 a          365 c          368 g          166 t
ORIGIN      
        1 aggaactcgg gctcgagact ttcccgacct ggtgcgaagg cagaaacgtg ttcgagcggg
       61 gcggcaaggc tcgcacctac tcgggcacga tccccaggct caatccggtt tccctggccg
      121 aggtcgggat ggccctgaag cggctcgaaa agatggccgc gaaggtggac gtcacgcagc
      181 cctgggccca gaccgatctg gcccgctggg atgccgagac cttcgccacc tgggcgcggc
      241 gcaacgcgaa gaccccggca gccagggacc tgcttcgact cgcgattcag gcggtctggg
      301 cggcggagcc ggaagatgtc tcgctgctcc acgccctctt ctacgtccag tcctccggcg
      361 ggtcactcga gaagatgctg gacgccgagg ggggcgccca ggactcacgg gtggtcggag
      421 ggacccagct gattgccgaa aggatggcgg ccgaactcgg cgacgccgtc catctcgggt
      481 ttccggtccg ccgggtccgc tggcagagga tgccgggtga tatcgaggat gccccggtcg
      541 agttcggccc tcatccggta acggtcgagg ccgacgagct gaccgtttcc gcccgggcgg
      601 cggtgatcgc gctcccgccc gcagtcgccg gacggctcga gtacgacccg atcatgccgg
      661 cccttcggga cgggctgacc caacgcatgg ttcagggaac ggtggtcaag tgccatgcga
      721 tctacgacga gcccttctgg cgggccgatg gcctgtccgg ccaggcgacc agtgccgacg
      781 gcccggtttc cgtcaccttt gacaactccc cgccctccgg cacccccggt gttctgctcg
      841 ccttcctcga aggaaacgcg gctcgccaag ccaccgatct acccccggcg gagcgccgcg
      901 agatcgtgct cgattgcctg gtccggctgt tcggaccaaa ggccgcgaac ccggagcggt
      961 tcgtggacaa ggcgtgggcc gccgacgagt tcgcgcgcgg ttgctacggc ggctatctgc
     1021 cccccggggc ctggctgatg tacggcgagg gcctcaggac ccc
//
LOCUS       DAOQMF010000772         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_134108_length_1062_cov_4.546177, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000772 DAOQMF010000000
VERSION     DAOQMF010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134108_length_1062_cov_4.546177"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1062)
                     /locus_tag="PLV77_11000"
     CDS             complement(<1..>1062)
                     /locus_tag="PLV77_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNC16420.1"
                     /translation="RPNIIYVHSHDTGRYVQPYGHHVLTPNMQLLADQGITFRRAFCA
                     APTCSGSRAALLTGQHCHNNGMMGLAHRGWKLNDYSQTMVHPLKEGGYRTILVGEQHI
                     SRDPHVIGYDEVIELSDNYAKRVAPSALEAIRRTSEPFFMSVGFFETHRDFAAPTSVR
                     DSLYSLPPENLPDTAETRSDMASFKESVRSLDQGIGALLNGLHATGLADRTLVICTTD
                     HGLAFPKAKATLLDGGIGVMMLMRGPNGFSGGKVIDSMVSHLDIYPTVCELAGVPPPD
                     FLQGRSLMPLVSGESAALHEELFSEVTYHAAYEPQRAVRTNRWKYIRRFDDRKRPVLA
                     NCDDSLTKDLLIAMGWGDR"
BASE COUNT          176 a          322 c          374 g          190 t
ORIGIN      
        1 cgcggtcacc ccaccccatt gcgatcaaca ggtccttggt caggctgtcg tcgcaattcg
       61 cgaggaccgg ccgcttgcga tcgtcgaacc ggcggatgta cttccagcga ttggtccgca
      121 ccgccctttg cggctcgtag gcggcgtggt aggtcacctc ggagaacagc tcttcgtgaa
      181 gagcggcgga ttccccgctg accaggggca tcagtgacct gccctgcagg aaatccgggg
      241 gcggcacccc ggccagttcg cagaccgtcg ggtagatgtc gaggtgggag accatcgaat
      301 cgatcacctt gccgccggag aaaccgttcg ggcctcgcat cagcatcatc acgccgatcc
      361 cgccgtcgag cagggtcgcc ttcgccttgg ggaaggcgag gccgtggtcg gtggtgcaga
      421 tgaccagggt ccggtcggcc aggccggtcg cgtggagacc gttgaggagt gcaccgatgc
      481 cctggtccag cgaacgcacg ctctccttga aggaggccat gtcactgcgc gtttcggccg
      541 tgtccggcag gttctccggc ggcagcgaat aaagcgagtc gcggaccgag gtcggggccg
      601 cgaagtcacg atgggtttcg aagaagccga ccgacatgaa gaagggctca cttgtccggc
      661 ggatcgcttc gagggcgctg ggggcaaccc gcttggcgta gttgtcgctc agctcgatca
      721 cctcgtcgta accgatcacg tgcgggtcgc gggagatgtg ctgctcgccg accaggatgg
      781 tccggtagcc gccctccttc aggggatgaa ccatcgtctg cgagtagtcg ttcagcttcc
      841 agccccggtg ggcgagcccc atcattccgt tgttgtggca atgctggccg gtcaggaggg
      901 ccgcccggct ccccgagcag gtcggggcgg cgcagaaggc ccgccggaaa gtgatcccct
      961 ggtcggccag cagctgcatg ttcggggtca ggacgtgatg gccgtagggc tggacatagc
     1021 ggcccgtgtc atgggaatgc acatagatga tgttggggcg gt
//
LOCUS       DAOQMF010000773         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_134217_length_1062_cov_2.473684, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000773 DAOQMF010000000
VERSION     DAOQMF010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134217_length_1062_cov_2.473684"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLV77_11005"
     CDS             <1..317
                     /locus_tag="PLV77_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16421.1"
                     /translation="RVAAEAAASGVLPVCAEHSGLAEVTATLAEEVPAVESMIGLPLG
                     PGAVAELAGKVNRWLALDPAERLEIGQNIAESADRNWSWQGVAKDVISASAGQVRKVV
                     QS"
     gene            395..>1062
                     /gene="coxB"
                     /locus_tag="PLV77_11010"
     CDS             395..>1062
                     /gene="coxB"
                     /locus_tag="PLV77_11010"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNC16422.1"
                     /translation="MVLAVIVLGVVQSVVLLQVDWFGDAASTAAGPIDTLLDVTIVVS
                     SFVFAIVCVALGYALIKWRAKPGDESDGLPIHGNTKLEIVWTIIPIIMVLGLAGYSWA
                     VLDDIEKTDADAIHINAYAQQFAWTFGYPESGNKWSEGELHVPVDRQIIIDLQAQDVL
                     HSFWVPEWRIKKDAVPGLETDAIITPDKEGTYQLICAELCGIGHTTMRAIVVVESEAK
                     YQAWL"
BASE COUNT          190 a          331 c          343 g          198 t
ORIGIN      
        1 atcgcgtcgc cgccgaagcg gccgcttccg gggtgcttcc ggtctgcgcc gagcactcgg
       61 gcctggccga ggtcaccgcc acgctggccg aggaagttcc cgccgtggaa tcgatgatcg
      121 ggctgccact cgggccggga gccgtggccg agctggccgg caaggtcaac cgctggctgg
      181 ccctcgaccc ggcggagcgg cttgagatcg gtcaaaacat tgccgagtcc gccgatcgga
      241 attggagctg gcagggggtc gcaaaggatg tgatttccgc atcagcaggc caagtgcgga
      301 aggtcgtcca gtcctgagcg cagggtcaga aagcattcgt ccccggctct aatagaatgg
      361 cggtcgcctc atgactcgtc tcccgatttt gaaaatggtg ctggccgtaa tcgtcctcgg
      421 cgtggtccag tctgtggttc tgctccaggt cgactggttc ggcgatgcag cttccaccgc
      481 ggccggtccg atcgacacgc tgctggacgt caccatcgtc gtctcgtcgt ttgtcttcgc
      541 gatcgtctgc gtcgccctcg gctacgccct gatcaagtgg cgggccaagc ccggtgacga
      601 aagcgacgga ctgccgatcc acggcaacac gaagctcgag atcgtctgga cgatcatccc
      661 gatcatcatg gtgctcggcc tggccggcta ctcgtgggcc gttctcgacg acatcgagaa
      721 gaccgatgcg gacgcgatcc acatcaacgc ctatgcccag cagttcgcct ggaccttcgg
      781 ttacccggag agcggcaaca aatggtcgga gggcgagctt cacgttccgg tcgaccggca
      841 gatcatcatc gaccttcagg cgcaggacgt tctgcactcc ttctgggttc cggaatggcg
      901 gatcaagaag gacgcggtgc cgggacttga aaccgacgcg atcattacgc cggacaagga
      961 aggcacctac cagttgatct gtgccgagct ctgcggcatc ggccacacga cgatgcgtgc
     1021 gatcgtggtc gtcgagtccg aagcgaagta ccaggcctgg ct
//
LOCUS       DAOQMF010000774         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_134264_length_1062_cov_2.013903, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000774 DAOQMF010000000
VERSION     DAOQMF010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134264_length_1062_cov_2.013903"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLV77_11015"
     CDS             <1..771
                     /locus_tag="PLV77_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC16423.1"
                     /translation="ARSGQWEYFFYIDVQGHPLANDFDQDFGDINVTTALTNSVNTYF
                     AQLGEKIGTGTLEDYMTKFGFNSKPQLDLPDGQMSTSGVFDLENDKLLTGDDPIDVGR
                     LAIGQERLLATPVQMAEVAATIANGGDLMRPQIWSRVMDADGRTVKSMDPEKQSDVMS
                     EETAGELTEAMKDVVNEGTGPAAALSGVDVAGKTGTAEIPDVDRCDGLPNQAWFVGFA
                     PADDPQIAGAAPVEGTDGQGGTVAAPIAAGVMQTLLGE"
     gene            807..>1062
                     /locus_tag="PLV77_11020"
     CDS             807..>1062
                     /locus_tag="PLV77_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC16424.1"
                     /translation="MRLSEGMVVDGRYRIDYLIGSGGMADVWLAEDLELPRRVALKVL
                     HERYARDPEFIARFRREAESAASLQHVNIVSIFDRGQVGDT"
BASE COUNT          202 a          331 c          352 g          177 t
ORIGIN      
        1 gcccgttccg gccagtggga atactttttt tacatcgatg tgcagggcca tccgcttgcc
       61 aacgacttcg accaggactt cggcgacatc aacgtgacca ccgccctgac caactcggtc
      121 aacacctatt tcgctcagct cggcgagaag atcggcaccg gcaccctcga ggactacatg
      181 acgaagttcg gcttcaactc gaagccgcag ctcgatctgc ccgacggcca gatgtcgacc
      241 agcggggttt ttgacctcga gaatgacaag ctgctgaccg gtgacgaccc gatcgatgtc
      301 ggtcgactgg cgatcggcca ggaaaggctg ctggccacac cggtccaaat ggccgaagtg
      361 gcggcgacga tcgccaacgg aggtgacctg atgcgcccgc agatctggtc cagggtcatg
      421 gatgccgacg gtcgcacggt caagtcgatg gacccggaga agcagtccga cgtgatgtcg
      481 gaggagaccg ccggcgagtt gaccgaggcg atgaaggatg tcgtcaacga gggcaccggc
      541 cccgcggccg cgctgagcgg ggtggatgtg gccggcaaga ccggcaccgc cgagatcccc
      601 gacgtggacc gttgtgacgg cctgccgaat caggcctggt tcgtcggctt cgccccggcc
      661 gacgatccgc agatcgcggg tgccgccccg gtcgagggca ccgacggaca gggcgggaca
      721 gttgccgccc cgatcgccgc cggtgtaatg cagactctct tgggtgagta ggacttgccg
      781 gacaaagacc gaaaagcaga acctagatga gactgagtga aggcatggtg gtggatggcc
      841 gctaccggat cgattacctg atcggctccg gcgggatggc cgacgtctgg ctggccgagg
      901 atctggagct gccgcgccgg gtcgccctca aggtgctcca cgagcgctat gcccgggacc
      961 ccgagttcat cgcccgtttc cgccgtgaag ccgaatctgc cgcttcgctc cagcacgtga
     1021 acatcgtttc gatcttcgat cgcggccagg tgggggacac ct
//
LOCUS       DAOQMF010000775         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_134424_length_1061_cov_2.934394, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000775 DAOQMF010000000
VERSION     DAOQMF010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134424_length_1061_cov_2.934394"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PLV77_11025"
     CDS             complement(<1..990)
                     /locus_tag="PLV77_11025"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNC16425.1"
                     /translation="MSATAPVRLSSAAARRIALAAQGFGRERPFPRVTMRQVQAMIDR
                     IGLLQIDSVNVLARAHLLPLYSRLGPYDIDLLEKASSKRPRRLVEYWAHEASFVPPET
                     HRLLRWRMDRADQDAWRTVRAAAEQPELLEDVVAEVERSGPVTAGQVAAAVDPDHVPD
                     KSNWGWNWPPVKSALEYLFWSGRITAAGRTSQFERRYDLPGRVLPGHVHEAETPEPEE
                     AIEGLLEISARAHGVGTARCLRDYFRLPARESKEAIERLAADGRLIEAEVEGFSAPAF
                     LHPEARRPRKVEARALLAPFDPLIFERRRLEELFGFHYRIEIYTPKEKRRHGYY"
BASE COUNT          160 a          374 c          352 g          175 t
ORIGIN      
        1 gtagtagccg tggcgacgct tctccttcgg ggtgtagatc tcgatccggt agtggaagcc
       61 gaaaagctcc tcgagtcgac gcctctcgaa gatcagcgga tcgaatggag cgagcagcgc
      121 ccgcgcctcg accttgcggg gtcgacgggc ctcggggtgc aggaaggccg gagccgagaa
      181 cccttcgacc tcggcctcga tcaggcgccc gtcggcggcg agccgttcga tcgcctcctt
      241 cgactcccgg gccggcaggc ggaagtagtc cctgaggcag cgcgccgtgc cgaccccgtg
      301 ggcccgggcg gagatctcca gcaatccctc gatcgcttcc tccggctcgg gagtttccgc
      361 ttcgtggacg tggccgggca gcacccgccc cggcaggtcg tagcgtcgtt cgaactggct
      421 ggttcgcccg gccgcggtga tccggccgga ccagaacagg tactcgaggg cgctcttgac
      481 cggcggccag ttccatcccc agttgctctt gtcggggaca tggtccgggt cgacggcggc
      541 cgcgacctga ccggcggtca ccggacccga tcgctccacc tcggcgacta cgtcctcgag
      601 cagttccggc tgttcggcgg cggcccgtac cgtgcgccag gcgtcctggt ctgcccggtc
      661 catccgccag cggagcagcc ggtgggtttc cggtggaacg aaggaggcct cgtgggccca
      721 gtactcgacc agccggcgcg gccgcttcga actcgccttc tcgagcagat cgatgtcata
      781 gggcccaagc cgggagtaga gcggcagcag gtgtgccctg gccagcacgt tgaccgagtc
      841 gatctggagc aggccgatcc ggtcgatcat cgcctggacc tgacgcatcg tcaccctcgg
      901 aaaggggcgt tcgcgaccga agccctgcgc ggccagggca atccggcgtg ctgccgccga
      961 actcagcctc accggggccg tggcactcat ccgaccaagc ctcgctccca acttgcgtca
     1021 tgtccgttca ctcgcacata attaccgcca acgccacttc c
//
LOCUS       DAOQMF010000776         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_134778_length_1060_cov_2.482587, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000776 DAOQMF010000000
VERSION     DAOQMF010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134778_length_1060_cov_2.482587"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..809
                     /gene="hisF"
                     /locus_tag="PLV77_11030"
     CDS             45..809
                     /gene="hisF"
                     /locus_tag="PLV77_11030"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNC16426.1"
                     /translation="MSDLKRIIPCLDVDNGRVVKGTNFVDIRDAGDPVELAERYDAEG
                     ADELVFLDITASHEGRDTIVDLARRTAENVFIPFTIGGGIRSVEDARAVLNAGADKVA
                     INSAALNRPELLTELAAVFGSQCVVIAIDAKRNEDGNYGVFLNGGRVETGRDAIEWAT
                     EATRLGAGEVLLTSMDRDGTNIGYEIELTRRVAEATDVPVIASGGAGELNHLVEAIRD
                     GKADAVLCASIFHYGHYTVEQAKEHMASHQIPVRVT"
BASE COUNT          213 a          357 c          334 g          156 t
ORIGIN      
        1 ttcgaaggca agttcacggt cgaagagggg atcgcggcgc tgaaatgagc gacctcaaga
       61 gaatcatccc ctgcctcgac gtggacaacg gccgcgtcgt caagggtacg aacttcgttg
      121 acattcgcga cgccggggat ccggtcgagc tggccgagcg ctacgacgcc gagggtgccg
      181 acgagctcgt cttcctcgac atcaccgcct cccacgaggg ccgtgacacg atcgtcgacc
      241 tggcccgtcg aaccgccgag aatgtgttca tccccttcac gatcggcggc ggcatccgtt
      301 cggtcgaaga cgcccgcgcc gtcttgaacg cgggggccga caaggtcgcg atcaacagcg
      361 ccgccctgaa ccgtccggaa ctgctgaccg agctggccgc ggtattcgga tcccagtgcg
      421 tggtgatcgc gatcgacgcg aagcgaaacg aggacggaaa ctacggggtc ttcctcaacg
      481 gcggccgggt cgagacggga cgcgacgcga tcgagtgggc aaccgaggcc acacgactcg
      541 gggccggaga agtcctcctg acaagcatgg accgcgacgg caccaacatc ggctacgaaa
      601 tcgaactgac ccgcagggtc gccgaggcaa ccgacgtgcc ggtcatcgcc tcgggcggag
      661 ccggcgagct caaccacctg gtcgaagcaa tcagagacgg caaggccgac gccgtcctct
      721 gcgcctcgat cttccactac ggccactaca cggtcgagca ggccaaggaa cacatggcct
      781 cccaccagat cccggtccgg gttacctaac cgcaagtcgg cgagcttcgc cccgcctcca
      841 ttccaaaccc gccggcccac agaagcacat ctgcaagtcg gcgagcttcg ccccgcctcc
      901 aattcaaacc cgccggccca caggagcact tgatggatgg gccggctcga ccggcagtag
      961 aaccggctag gcgccagctg agaccagctt tgtggcgagc tggtcggggc cgtcgccggc
     1021 gtcgattcgg attcggttga gctcgaaggg cattgacttt
//
LOCUS       DAOQMF010000777         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_135485_length_1057_cov_2.637725, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000777 DAOQMF010000000
VERSION     DAOQMF010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135485_length_1057_cov_2.637725"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLV77_11035"
     CDS             <1..648
                     /locus_tag="PLV77_11035"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNC16427.1"
                     /translation="DNDLNATDYTFGFDTAVNIVMESLDRLHTTATSHHRCLVVEVMG
                     RHAGWIALHGGMAGGADQILVPGEEFDLEQVCRQVNRSFEAGVAPIVVASEGAIPAEG
                     SELTTAEGTDAFGHVRLGGIGGWLAERIEENTGHETRSVVLGHLQRGGTPTAFDRVLA
                     TRLGSRAIQAANEGEWGTMTTLRGTSIELAPLADAVDESKVVPPERLREAQALFD"
     gene            complement(703..>1057)
                     /locus_tag="PLV77_11040"
     CDS             complement(703..>1057)
                     /locus_tag="PLV77_11040"
                     /inference="COORDINATES: protein motif:HMM:NF019938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HNC16428.1"
                     /translation="AGVGGPIAGGKQYMSWVHRHDEIGILLWALENESVSGPVNATAP
                     NPVTNKEFSKALGKQLGRPSFLPVPGFAIDIVKGGGVGHAAREGQRVFPKKATDGGYE
                     FKQPELAGALANLLD"
BASE COUNT          179 a          345 c          360 g          173 t
ORIGIN      
        1 gacaacgacc tgaacgccac cgactacacc tttggcttcg ataccgcggt caacatcgtg
       61 atggaatccc tggaccggct gcacacgacc gcaaccagtc atcaccgctg cctggtggtc
      121 gaggtgatgg gtcgtcacgc cggctggatc gcgcttcacg gcgggatggc cggtggcgcc
      181 gaccagattc tcgtgccggg agaggagttc gacctcgagc aggtctgccg tcaggtcaac
      241 cgcagcttcg aggccggggt tgccccgatc gtcgtcgctt ccgagggcgc gattccggcg
      301 gaaggcagcg agctcaccac ggccgagggg accgacgcct tcggtcacgt gcggctcggc
      361 ggcatcggcg gctggctggc ggaacggatc gaggagaaca cggggcacga gacgaggtcg
      421 gtcgtgctcg gccatctcca gcggggtgga accccgaccg ccttcgaccg ggtgctggcc
      481 acgcggcttg gaagccgggc gatccaggcg gccaacgaag gcgaatgggg caccatgacc
      541 acgcttcggg gtacctcgat cgaactcgcc ccgctggccg acgccgtgga cgaatcgaaa
      601 gtcgtcccgc cggagcggct tcgcgaagca caggctctct tcgactgaat cccgaatcat
      661 cctgccctcg ggaaagggaa ggaagttcac aacggggatc gctcagtcga gcaggttggc
      721 gagggcgccg gccagttccg gctgcttgaa ctcgtaaccg ccgtcggtcg ccttcttggg
      781 gaagaccctc tgaccctcac gggcggcatg gccgactccg ccgcccttga cgatgtcaat
      841 cgcgaagccg ggcacgggca ggaacgaggg ccggcccagc tgcttgccga gtgctttcga
      901 gaattccttg ttggttaccg ggttaggtgc cgtcgcgttg accggtccgc tgaccgactc
      961 gttttcaagg gcccagagca ggatcccgat ctcgtcgtgc cggtggaccc aggacatgta
     1021 ctgcttgccg ccggcgatcg gcccgccaac tccggcc
//
LOCUS       DAOQMF010000778         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_136271_length_1054_cov_3.060060, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000778 DAOQMF010000000
VERSION     DAOQMF010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136271_length_1054_cov_3.060060"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..>1054
                     /gene="tgt"
                     /locus_tag="PLV77_11045"
     CDS             146..>1054
                     /gene="tgt"
                     /locus_tag="PLV77_11045"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNC16429.1"
                     /translation="MRHDPARLTFEIHARDGDARRGTLLTPHGPVETPAFIPLATKGS
                     VRGMSTREIEGMGYQMVLGNTYHLLVAPGPERIAEAGGLHRFMGWEKAIITDSGGFQV
                     FSLAHGGVAEEIKGSGRKMYGLAKTLGIEEEGVRFRSYKDGSELMLSPEESMRVQAAL
                     ASDIALVFDECTPFHADREYTARSTERTHRWLKRCLDWHEANGPAGQAVFGIIQGGTH
                     ADLRRESCEAVSSSAVDGISIGGTLGRDKEEMAEVLGFTVPNLPVEAPKHLLGIGEVD
                     DLMRGVALGLDVFDCAVPTRLARHGMA"
BASE COUNT          177 a          356 c          352 g          169 t
ORIGIN      
        1 ctccagatgc tcgggattca gcagccggta gagatgaccg gccgcagcct gatcgagccc
       61 gcctgatccg cgagatcccg gttcgcgccg cataccggca caaacgggaa tctcgcgtgg
      121 ttttccggac ggatagggtg gcctgatgcg ccacgacccg gcccggctga ccttcgagat
      181 ccacgcccgc gatggcgatg cccgtcgcgg cacccttctg acaccccacg gcccggtcga
      241 aacgccggcc ttcatccccc tcgcaaccaa gggctcggtt cgcggcatgt ccacgcggga
      301 aatcgaggga atgggttacc agatggtgct gggcaacacc taccacctgc tggtcgctcc
      361 ggggccggag cggatcgccg aagccggcgg gctccaccgg ttcatgggct gggagaaggc
      421 gatcatcacc gactcgggcg gcttccaggt cttctcgctg gcccacggcg gggtggccga
      481 ggagatcaag ggctcgggac gcaagatgta cgggctcgcc aaaaccctgg ggatcgagga
      541 ggagggagtg cgattccgtt cctacaagga cggctccgag ttgatgcttt caccggagga
      601 gtcgatgcgg gtccaggccg cgctcgcttc cgacatcgcc ctggtcttcg atgaatgcac
      661 ccccttccac gccgaccgtg agtacaccgc ccgctcgacc gagcggacgc accgctggct
      721 gaaacgctgc ctcgactggc acgaagccaa tggcccggcc gggcaggcgg tcttcgggat
      781 catccaggga ggcacccacg ccgacctgcg cagggagtcc tgcgaggcgg tctcctcatc
      841 tgcggttgac gggatctcga tcggcggcac cctcggccgc gacaaggaag agatggccga
      901 agtgctcggt ttcacggtgc cgaacctgcc ggtcgaggct cccaagcacc tgctcgggat
      961 cggcgaagtt gatgacctga tgcgaggcgt ggcgctcggc ctcgatgtct tcgactgtgc
     1021 cgttccgacc cggctcgccc gccacggcat ggcc
//
LOCUS       DAOQMF010000779         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_138345_length_1046_cov_4.596367, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000779 DAOQMF010000000
VERSION     DAOQMF010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138345_length_1046_cov_4.596367"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLV77_11050"
     CDS             complement(<1..338)
                     /locus_tag="PLV77_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16430.1"
                     /translation="MRLINNPVRRFDFVQKLEWFLLSAVTMILIIRTQLWLTHYPQLG
                     GGGLHIAHLLYGGLFMVIAIWLGQIYLNRWGRTVQAILGGIGFGFFIDELGKFITEDN
                     NYFFKPAAGI"
     gene            complement(358..>1046)
                     /locus_tag="PLV77_11055"
     CDS             complement(358..>1046)
                     /locus_tag="PLV77_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC16431.1"
                     /translation="FLEIVKRSWSGETFDHEGEFFHLEKGGLRLGFGEVDPPRVYFGG
                     ASPPALEVAAEYADTWLTWGEPVDTVAGRVAPVREKAEAAGREISCGLRIHVISRDTE
                     EEAWLEADRILEAIDPEQVAAAREKFARMESTAQARMTGLSHSSEGDALDRDALVIGP
                     NLWAGVGLVREGAGTAIVGSHQQVAERLLEYRDIGIDEFILSGYPHLEEALNFGENVR
                     PLLDAATVFA"
BASE COUNT          183 a          335 c          353 g          175 t
ORIGIN      
        1 atgattccgg cggccggctt gaagaagtag ttgttgtcct cggtgatgaa cttgcccagt
       61 tcatcgatga agaagccgaa accgatcccg ccgaggatcg cctggacggt ccggccccag
      121 cggttcaggt aaatctggcc gagccagatc gcgatcacca tgaagaggcc gccgtagagc
      181 aggtgggcga tgtgaaggcc accgccgccc agctgcgggt agtgggtcag ccagagctgc
      241 gtgcggatga tcaggatcat cgtcaccgct gacagcagga accattcgag cttctggacg
      301 aagtcgaacc gcctgaccgg attgttgatc agccgcacgg gctgactgta gcttcgctca
      361 ggcgaagacg gtcgcggcgt cgagcagcgg gcgcacgttc tcgccgaagt tgagcgcttc
      421 ctcgagatgc ggatagccgg agaggatgaa ctcgtcgatg ccgatgtcgc ggtattcgag
      481 caggcgctcg gcaacctgct ggtggctgcc gacgatcgcg gtgccggccc cctcgcggac
      541 cagcccgacc ccggcccaga ggttgggtcc gatgaccagg gcatcccggt cgagcgcgtc
      601 tccttcggaa gagtggctga ggccggtcat ccgggcctgg gcggtcgatt ccatgcgggc
      661 gaacttctcg cgcgcggcgg caacctgttc gggatcaatc gcctcgagga tgcggtcggc
      721 ctcgagccag gcctcttcct cggtgtcacg gctgatcacg tgaatgcgca gtccgcaact
      781 gatctcgcga ccggcagcct ccgccttctc cctgaccggc gcgacccgcc cggccaccgt
      841 gtcgaccggc tcgccccagg taagccaggt gtcggcgtat tcggcggcga cctcgagggc
      901 cggcggagag gccccgccga aatagacccg gggcggatcg acctcaccga acccgaggcg
      961 caggccgccc ttctcgaggt gaaagaactc gccttcgtgg tcgaaggttt cgcccgacca
     1021 ggagcgcttg acgatctcga ggaact
//
LOCUS       DAOQMF010000780         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_138658_length_1045_cov_3.060606, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000780 DAOQMF010000000
VERSION     DAOQMF010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138658_length_1045_cov_3.060606"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1045)
                     /locus_tag="PLV77_11060"
     CDS             complement(<1..>1045)
                     /locus_tag="PLV77_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC16432.1"
                     /translation="AVASLGAVWSSCSPDFGAGAVTDRLAQIEPRVLLAVDGYNYGGK
                     GFDRRGVVSDLVAGMPTVEQVVILPYLEPDPDLTPLPGAITWNELLSKDPGAELTFTR
                     VPFDSPLWILYSSGTTGLPKPIVQSQGGILLEHLKKMHLHLDAGEGDRIFWFTTTGWM
                     MWNFLVSCLLTDAAIVLYDGNPGHPDMSALWQLASDAGVTCFGTSASYISACMKDGVE
                     PGADGRDLSKLRSVGSTGSPLAPEGFDWVYEHIGKDTWLFSTSGGSDLCTAFVGGVPT
                     LPVYRGELQARALGAAIESWDEEGNPHIGEVGELVITKPMPSMPLFLWGDEDGSRYHD
                     SYFDTYPGGRADGR"
BASE COUNT          199 a          328 c          363 g          155 t
ORIGIN      
        1 gcgcccatcg gcacggccgc ccgggtaggt gtcgaagtag ctgtcgtggt agcgggatcc
       61 gtcctcgtcg ccccagagaa acagcggcat cgacggcatc ggcttggtga tgaccagttc
      121 cccgacctcg ccgatatgcg ggttgccctc ttcgtcccag gactcgatcg ccgccccgag
      181 cgcccgggcc tgcagctcgc cccggtagac cggaagggtg ggaacgccac cgacgaaggc
      241 cgtgcagagg tcggaaccac ccgaggtcga aaacagccag gtgtccttgc cgatgtgctc
      301 gtacacccag tcgaagccct cgggggcgag cggcgagccg gtcgagccga ccgagcggag
      361 ctttgagagg tcacggccgt cagcgccggg ctcgaccccg tccttcatgc aggccgagat
      421 gtaggaggcg gaggtgccga agcaggtcac cccggcatcc gaagcgagct gccagagagc
      481 tgacatgtcg gggtgaccgg ggttgccgtc ataaagcacg attgccgcgt cggtgagcag
      541 acaggagacg agaaagttcc acatcatcca gccggtggtc gtgaaccaga agatccggtc
      601 accctcaccg gcatcaaggt ggaggtgcat cttcttcagg tgctcgagca ggatgccgcc
      661 ctggctctgg acgatcggct tcggcagccc ggtggtgccg gaggagtaga ggatccacag
      721 gggggagtcg aatggcaccc gggtaaacgt caactcggcc ccgggatcct ttgaaagcag
      781 ttcgttccag gtaatggcgc cagggagcgg ggtcaggtcg ggatccggtt ccaggtaggg
      841 caggatcacg acctgctcga ccgtgggcat accggcaacc agatcggaaa ccacgccgcg
      901 acggtcaaat cccttgccgc cgtagttgta gccgtcgacc gcgagcagga ctctgggctc
      961 gatctgggcc agacggtcgg tcacggcccc ggcaccgaaa tccggggagc agctcgacca
     1021 gaccgcgccc aggctggcaa cggcg
//
LOCUS       DAOQMF010000781         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_140533_length_1039_cov_1.305894, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000781 DAOQMF010000000
VERSION     DAOQMF010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140533_length_1039_cov_1.305894"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLV77_11065"
     CDS             <1..890
                     /locus_tag="PLV77_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16433.1"
                     /translation="GEVPAPGSPAEREAVEQLFFHWIEASPPTFFHRPDMMLATAGAA
                     VGLVAAAWRLAGGRLNLPQGERARGVWLAVAMVASVFICTLVLLALGTDIFGARNLAP
                     AWVGMPFLFGSVFVAAGRRAGWVFLILMIAGFMVSTIRLMDPNRSTINYSRVADQLDD
                     SGEKGTILDASIASPAPLTSLDAYLETDLPVFKMTAIEDRPDYIKQLFATYDAQKILD
                     RAFSGAGPVRVVTADDFRAPREITSGGAAGGYEIQVNGQTAVIPPGWEPVSSHRYPGL
                     EPLTVTVFERSGQTGKSDR"
BASE COUNT          157 a          357 c          346 g          179 t
ORIGIN      
        1 gcggggaggt gccggcgcca ggttccccgg cggagcggga agcggtcgag cagctcttct
       61 tccactggat cgaggccagc cccccgacct tcttccaccg gccggacatg atgctcgcca
      121 cggccggggc ggcggtcggg ctggtcgctg cggcctggcg cctggctggt ggccgactga
      181 atctgccgca gggcgaaagg gcgcgcggag tctggctggc ggtggcgatg gtggcctcgg
      241 tcttcatctg cactctggtc ctgctcgccc tcggaaccga catcttcgga gcccgcaacc
      301 tggctccggc ctgggtcggc atgccattcc tcttcggttc ggtcttcgtc gccgccggcc
      361 gccgggccgg ctgggtcttt ttgatcctga tgatcgccgg gttcatggtc tcgaccattc
      421 gcctgatgga cccgaaccgc tcgacgatca attattcccg ggtcgccgac cagctcgacg
      481 actcgggtga gaaggggacg atcctcgacg cctcgatcgc cagccccgcg cccctgacct
      541 cgctcgacgc ctatctggaa accgacctgc cggtcttcaa gatgaccgcg atcgaggacc
      601 gccccgatta catcaagcag ctcttcgcca cctacgacgc ccagaagatc ctcgaccgtg
      661 ccttcagcgg cgcgggtccg gtgcgggtgg tgaccgccga tgacttccgg gcgccccggg
      721 agatcacgag cggcggggcg gctggcggat acgaaatcca ggtcaacgga cagacagccg
      781 ttatcccgcc cggctgggaa cccgtctcca gccaccggta cccgggcctg gaaccgctca
      841 ccgtcacggt cttcgagcga agcggccaaa ccggaaagag cgaccggtga acgagcggat
      901 cgagaaatgg ctgggagcgg gccggcgatt tccggtcctg atcggcctgc tggccgaggc
      961 cggtttcggc ctgcggatga tccagatggg caaccccctt ttcggcgacg agctttccac
     1021 cctctggatc gtcgggaac
//
LOCUS       DAOQMF010000782         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_140586_length_1038_cov_3.658189, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000782 DAOQMF010000000
VERSION     DAOQMF010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140586_length_1038_cov_3.658189"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..>1038)
                     /locus_tag="PLV77_11070"
     CDS             complement(74..>1038)
                     /locus_tag="PLV77_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC16434.1"
                     /translation="DGDPGSQSETSVVPASISGTDPLLIDPMLNGSPGKSCSYLIQGE
                     KNVLIDPGSANAAPRIIEALKSNRIKVDLILLTHTHPDAAAGVGVVAREFPNAEIASP
                     AGATEQLADPSAMVSGMRKVYGEKVEKLYGLPAPVDSIRIHAVGDGEQVDLGDRAIEA
                     IATPGHTAAHMSYLDPESSSLFCGDALGIQLPGSSVVRPSTPPWDFSLEDSLDSIERL
                     AGVEAETVYLAHYGAARPGPTEIFDRAADSLERWHKSFLKKREQTDSEEDLSRQFNAC
                     LEASLEPIAPSVRRDLETVSPAWLNLAGLSAEQARLNGRLSDAA"
BASE COUNT          167 a          322 c          367 g          182 t
ORIGIN      
        1 catctggcgg ggcgagccgt ggctggagct cggcctcgaa ggtgaccggg tggtaatcct
       61 gcccggttag ggttcaggcc gcgtcggaaa ggcggccgtt gaggcgggcc tgttcggcgg
      121 agagcccggc caggttcagc caggccgggc tgaccgtctc gaggtcgcgg cgaaccgaag
      181 gggcgatcgg ctccaggctc gcttcgaggc aggcattgaa ctgccgggag aggtcctctt
      241 cgctgtcggt ttgttcccgc ttcttcagga acgacttgtg ccagcgctcg agtgaatcgg
      301 cggcccggtc aaagatctcg gtcggcccgg gacgagccgc cccgtaatgg gcgagataga
      361 ccgtttcggc ttcgaccccg gccagcctct cgatcgaatc cagcgaatcc tcaagcgaga
      421 aatcccaggg cggggtggac ggccggacga cgctgctgcc gggcagctgg attccgagcg
      481 cgtccccgca gaaaagcgat gaactctccg ggtcgaggta agacatgtgc gcggcagtgt
      541 ggcccggagt cgcgatcgcc tcgatcgccc ggtcgcccag atcaacctgc tcgccgtcac
      601 caaccgcgtg gatccgaatc gaatcgaccg gtgccggaag cccgtaaagc ttctcgacct
      661 tttcgccgta gaccttgcgc atcccggaaa ccatggccga gggatcggca agctgctcgg
      721 tcgctccggc cggcgaggcg atttccgcgt tcgggaattc gcgggcgacg acaccgaccc
      781 cggcggccgc gtccgggtgg gtgtgcgtga ggaggatcag gtcgaccttg atccggttcg
      841 atttcagtgc ctcgatgatc cggggcgcgg cattcgccga tcccgggtcg atcaggacgt
      901 tcttctcgcc ctggatcagg taggagcagg acttgcccgg gctcccgttc agcattggat
      961 cgatcagcag cggatcggtg ccggagatcg aggcggggac caccgaggtt tcggactggc
     1021 ttccggggtc accgtcag
//
LOCUS       DAOQMF010000783         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_141175_length_1036_cov_2.777778, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000783 DAOQMF010000000
VERSION     DAOQMF010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141175_length_1036_cov_2.777778"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1036
                     /locus_tag="PLV77_11075"
     CDS             <1..>1036
                     /locus_tag="PLV77_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16435.1"
                     /translation="LPEGGPELLGRAALIGRDFDLGILGRTTSDDDDRIIELLDTAVS
                     AGLLDESSAVPGRYSFVHALVRSSLSDSFSLTRRAAIHRRIGEAIEQRNQNRFGGQRN
                     DDLPVLAWHFSQSGPAEADRAINYANQAARQAEERLAYDEAVDFYDAAINACRADEPV
                     DQGLLAELLLSRAEAEWRMALLPQSGRTFSEAAAAARESGIPELLARAAMGTTWGSWD
                     TFESDREEQGRLLREALEAIGPGDSPYRAQLLSSLAQILYFSDGRSAEAREMALEGTG
                     MADRLDDPYTFFVSNIAATFLRWEPDGTESRLPMADRLVKVAESSEDPEDLAEALSWR
                     SVVQVCLGHRA"
BASE COUNT          168 a          350 c          359 g          159 t
ORIGIN      
        1 ctgcccgaag gggggcccga gctgctcggc cgggcggccc tgatcggccg cgattttgac
       61 ctcggcattc tcggccgcac gaccagcgac gatgacgacc ggatcatcga actgctcgat
      121 accgcggtca gcgccgggct gctggacgag tcgtccgcgg tgcccggccg ctactccttc
      181 gttcacgcgc tggttcgcag ctcgttgagc gacagcttca gcctgacccg gcgggccgcg
      241 attcaccgcc ggatcggcga agcgatcgaa cagcgcaacc agaaccgttt cggtggccag
      301 cggaacgacg atctgccggt gctcgcctgg catttctcgc aatccggccc ggccgaagcc
      361 gaccgcgcaa tcaattacgc aaaccaggcg gcaagacagg ccgaggagcg gctcgcctac
      421 gacgaggcgg tggatttcta tgacgcggcg atcaacgcct gccgcgccga cgaaccggtc
      481 gatcagggcc tcctggccga gctccttctg agccgggccg aggcggagtg gcggatggcc
      541 ctcctgccgc aatcgggccg gaccttcagc gaggcggcgg cagcggcgag ggaatccggg
      601 attccggagc tgcttgcgcg ggccgcgatg ggcaccacct ggggcagctg ggacaccttc
      661 gaaagcgacc gggaggagca gggccgcctg ctgcgggagg cgctcgaggc gatcggtccc
      721 ggggacagcc cgtaccgggc ccagctgctg tcatcgctcg cccagattct ctacttcagc
      781 gacggtcgtt ccgccgaagc ccgggagatg gccctggaag gcaccgggat ggccgaccgg
      841 ctggatgacc cgtacacgtt tttcgtctcc aacattgcgg ccacctttct ccgctgggaa
      901 cccgacggga ccgaatccag gctgccgatg gcagaccggc tggtcaaggt ggccgaatct
      961 tccgaggatc cggaagacct ggccgaagcc ctgagctggc ggtcggtcgt tcaggtctgt
     1021 ctcggtcacc gggccg
//
LOCUS       DAOQMF010000784         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_142270_length_1032_cov_3.531218, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000784 DAOQMF010000000
VERSION     DAOQMF010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142270_length_1032_cov_3.531218"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /gene="folK"
                     /locus_tag="PLV77_11080"
     CDS             complement(<1..264)
                     /gene="folK"
                     /locus_tag="PLV77_11080"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNC16436.1"
                     /translation="MSTDRPVYLGLGSNIGDRFGYLRDAIGRLEGKGLTVEAVSSAWE
                     TEPVGEILDQPDFLNAAIRVRSGLEPEELLDLLKQVEAELGRET"
     gene            complement(254..640)
                     /gene="folB"
                     /locus_tag="PLV77_11085"
     CDS             complement(254..640)
                     /gene="folB"
                     /locus_tag="PLV77_11085"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNC16437.1"
                     /translation="MLQGNDGEVIIRLEGVIAHTNHGVTAAEQEVGQRMVFDLEMEPA
                     ECRATATDEVLDTVDYGEVTAWLIESAMDTRYRTLERLVTVLAEGLLENFRVDAVRLR
                     ATKPVPPVPVTMDGASVEIFRRRDEY"
     gene            complement(650..>1032)
                     /locus_tag="PLV77_11090"
     CDS             complement(650..>1032)
                     /locus_tag="PLV77_11090"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNC16438.1"
                     /translation="EVAEFLAVQVGLAETAGVPRERIWIDPGIGFGKTVDHNLSLLRW
                     TGAFARMDLKVLVGPSRKSFIGKLSNDASESRRLGGTIAACLAAMEGGAEMVRVHDVA
                     EVAQAIRVASAIGSANGEPGGGPE"
BASE COUNT          173 a          361 c          327 g          171 t
ORIGIN      
        1 ggtctcgcgg ccgagttccg cttcgacctg cttgagcagg tcaagcagct cttccggctc
       61 gagaccgctg cggacccgga ttgcggcgtt gaggaaatcc ggctggtcga ggatctcgcc
      121 gaccggttcc gtttcccagg cggaggaaac ggcctcgacg gtcaggccct tgccttcaag
      181 ccggccgatc gcgtcgcgca ggtaaccgaa ccggtcgccg atgttggaac cgagtccgag
      241 ataaaccgga cgatcagtac tcatcgcgac gacggaagat ctcgaccgag gcgccgtcca
      301 tggtcaccgg caccggcggc acgggcttgg tcgcccgaag ccggacggcg tcaacccgga
      361 aattctcgag caacccctcg gccagcaccg tcaccagcct ctcgagggtg cggtagcggg
      421 tgtccatcgc cgactcgatc agccaggcgg tcacctcgcc gtagtcgacc gtgtccagta
      481 cctcgtcggt ggcggtggcc cggcattcgg ccggttccat ctcgaggtcg aagaccatgc
      541 gctggccgac ctcctgttcg gctgcggtca ccccgtggtt ggtgtgggcg atcacgccct
      601 cgaggcggat gatcacttcg ccgtcatttc cctgaagcat gaggggaatc tactccgggc
      661 cgccgcccgg ttcaccgttc gcggaaccga tggccgaagc cacccggatc gcctgggcga
      721 cctcggcgac atcgtgaacc cggaccatct ctgccccgcc ctccatcgcg gccaggcagg
      781 cggcgatcgt gccgcccagc cgacgggatt ccgacgcatc gttcgagagc ttgccgatga
      841 acgacttgcg cgaaggaccg accagcacct tcaggtccat gcgggcgaac gccccggtcc
      901 acctgagcag ggacaggttg tgatcaaccg tcttgccgaa tccgatgccg ggatcgatcc
      961 agatcctctc gcgtggaacc cccgccgtct cggccagacc gacctggacc gcgaggaact
     1021 ccgcgacttc gg
//
LOCUS       DAOQMF010000785         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_143129_length_1029_cov_3.756674, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000785 DAOQMF010000000
VERSION     DAOQMF010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143129_length_1029_cov_3.756674"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..660
                     /locus_tag="PLV77_11095"
     CDS             154..660
                     /locus_tag="PLV77_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC16439.1"
                     /translation="MSGGGDPAAFAFASRWEVSAGRQAVWDALVDFNRWPVWWPALEE
                     VIETIHGDEEGIGQKAIAVWRGPVGYTLKMAIEAVEKVHPEFLRGVATGDVVGEGMWT
                     LATTADQWTSIEFDWNVRANRKWMEFLAPLARPLFVSSHDHVMKEGAEGLAGHLSCEM
                     RDFSASVG"
     gene            892..>1029
                     /locus_tag="PLV77_11100"
     CDS             892..>1029
                     /locus_tag="PLV77_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC16440.1"
                     /translation="MKLKPLGDRLIVKPVDEEETTASGLVLPDTAKEKPQKGKVVAVG
                     DG"
BASE COUNT          201 a          307 c          331 g          190 t
ORIGIN      
        1 cgaatttcgc cgggtcgagg aaacacggga cttcctccag aagatctacg accgcgagat
       61 cgaggaagac gcgatcgtga ccatcgtcca cttctcccag atcgtcgaac ccggcctgac
      121 cggctacggc accgtcccct cgcccaacta gccatgtcgg gagggggcga cccggcagct
      181 ttcgcctttg cttcccgctg ggaggtttcg gccggtcgcc aggcggtgtg ggatgccctg
      241 gtcgacttca accggtggcc ggtctggtgg cctgcgctcg aagaggtgat cgagacgatc
      301 cacggtgacg aggaagggat cggccagaag gcgatcgcgg tctggcgcgg accggtcggt
      361 tacaccctga agatggcgat cgaagcggtc gagaaggtcc acccggagtt tcttcgcgga
      421 gtggcgaccg gcgacgtggt cggggaaggt atgtggaccc tcgcgaccac ggcagaccag
      481 tggacctcga tcgaattcga ctggaacgta cgggccaacc gcaagtggat ggaattcctc
      541 gccccgctcg cacgccccct cttcgtctcc agccacgacc atgtgatgaa agagggagcc
      601 gaaggcctcg ccggtcatct ctcatgcgag atgcgtgact tttcggcttc cgtcggctga
      661 gcccctctgc ttgcggactg gagccgggtt tccccggctt gagtcgagtg gactcagatt
      721 tttcacattt cggggttgac cgacggtctc aattctgtat ggttgtcagt tgctggcact
      781 ctccccccga gagtgccaag agactgaggc gcccgaatca tcgatttccc ggcgccagaa
      841 gcgatgtatg gaacctgaaa cacgtatgaa catgaccatc ggaggtaatc aatgaagctg
      901 aagcccctcg gggaccggct tatcgtcaag ccggtagatg aagaggagac gaccgccagc
      961 ggtctggtcc tcccggacac cgccaaggaa aagccgcaga agggcaaggt cgtcgctgtc
     1021 ggtgacggc
//
LOCUS       DAOQMF010000786         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_143153_length_1029_cov_3.207392, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000786 DAOQMF010000000
VERSION     DAOQMF010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143153_length_1029_cov_3.207392"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLV77_11105"
     CDS             complement(<1..281)
                     /locus_tag="PLV77_11105"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HNC16441.1"
                     /translation="MAGYTPATDPERAEMLARIGVDSIDDLFAQIPEEVRLGRPLDLA
                     DGLSESEVFDRLDTMAMQNFGGSGTTFLGAGMYDHYVPAIVEAIISRSE"
     gene            complement(288..674)
                     /gene="gcvH"
                     /locus_tag="PLV77_11110"
     CDS             complement(288..674)
                     /gene="gcvH"
                     /locus_tag="PLV77_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNC16442.1"
                     /translation="MTEASYPEDLKYHPEHDWARIEGGEATLGITWYAQDSLGEVVFF
                     EGPEIGSEVTKDQPYAEVESVKAVSDVIAPVSGEVIAVNDSLGDAPEAINADPYETGW
                     LIKVRLSDPSEADSLMDAAAYAASLE"
     gene            complement(738..>1029)
                     /locus_tag="PLV77_11115"
     CDS             complement(738..>1029)
                     /locus_tag="PLV77_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNC16443.1"
                     /translation="GSDKVAVFRANGTDEKLAPFVIEGAGIPRQGNPVMLGEKQVGEV
                     TSGTFSPSLEQGIGMAYLRTDLAEPGTEVEVDVRGKRRTARVSSKPLYKKER"
BASE COUNT          172 a          331 c          314 g          212 t
ORIGIN      
        1 aactcggagc ggctgatgat tgcttcaacg atcgcgggca cgtagtggtc gtacatcccg
       61 gccccaagga aggtggtgcc gctgccgccg aagttctgca tcgccatcgt gtcgaggcga
      121 tcgaagactt cggattcgct gagaccgtcg gcgaggtcga gcggccggcc cagccgcacc
      181 tcctcgggga tctgcgcgaa cagatcatcg atcgagtcca ccccgatccg ggccagcatc
      241 tcggcccgct cgggatctgt agccggtgtg taccccgcca aggttggcta ctccaggcta
      301 gcggcgtagg ccgccgcgtc catcagtgaa tctgcttcgg acggatcgct gagacgaacc
      361 ttgatcagcc agccggtctc gtagggatcg gcgttgattg cttccggggc atcacccagg
      421 ctgtcgttca cagcgatcac ttcgccggag accggcgcta tcacgtcgga gacggccttg
      481 accgattcga cttccgcgta gggctgatcc ttggtcactt ccgagccgat ctcgggaccc
      541 tcgaagaaaa caacctcgcc gagcgagtcc tgggcgtacc aggtgattcc gagggtggcc
      601 tcgccgcctt cgatcctcgc ccagtcgtgc tcggggtggt atttcaggtc ttcggggtag
      661 ctcgcctcag tcaattcatc gctcccttta caagaaggag cgatgaattg tcttcggggt
      721 agctcgcctc agtcaattca tcgctccttc ttgtaaaggg gtttggagct tacgcgggcc
      781 gtccgacgct ttccacgcac gtcgacctcg acttcggtac caggttcggc cagatcggtc
      841 cgcaagtatg ccatgccgat gccctgctcc agcgaaggcg agaaagtgcc gctggtcacc
      901 tcgccgacct gtttttcgcc cagcatgacc gggttgccct gtcgcggaat cccggccccc
      961 tcgatcacga acggggcgag cttctcgtcg gttccgttgg cgcggaagac ggccaccttg
     1021 tcggaaccg
//
LOCUS       DAOQMF010000787         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_143174_length_1029_cov_2.932238, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000787 DAOQMF010000000
VERSION     DAOQMF010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143174_length_1029_cov_2.932238"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLV77_11120"
     CDS             complement(<1..381)
                     /locus_tag="PLV77_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNC16444.1"
                     /translation="MTDLKLAEGEHLFTSESVTEGHPDKIADQISDTILDACLSQDPH
                     SRVACETLVNTGMAVISGELTTSAQIDITDLVRKTVCEIGYDKGEYGYDGNNISVLVS
                     MDKQSPDIAQGVDTGIETRTGGSSD"
     gene            601..>1029
                     /locus_tag="PLV77_11125"
     CDS             601..>1029
                     /locus_tag="PLV77_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNC16445.1"
                     /translation="MAMVRWEPSRELEAFQTDMNRLFDSFFRSGSNSTVRRWAPATDL
                     TEDGNDLVLRLDLPGLSDEDVAVEVEGNVLTVSGERRDERETKEGSTYRLERSFGKFS
                     RSFPLPEGTDPDAIEGGIDKGVLELRIPKPTEKQPRRVELG"
BASE COUNT          208 a          291 c          329 g          201 t
ORIGIN      
        1 atccgaagaa ccgccggtac gggtttcgat ccccgtgtcc acgccctggg cgatgtccgg
       61 ggactgcttg tccatcgaga ccagcaccga gatgttgttg ccgtcatagc cgtactcgcc
      121 cttgtcgtaa ccgatctcgc agaccgtctt gcggaccagg tcggtgatgt cgatctgggc
      181 ggaggtcgtc agctcgccgg agatcacggc catgccggtg ttgaccaggg tctcgcaggc
      241 aacccgggaa tgcggatcct gactgaggca tgcgtcgaga atcgtgtccg agatctggtc
      301 ggcgatcttg tcgggatggc cttcggttac ggattctgag gtgaagaggt gttcaccctc
      361 ggcaagcttc agatcggtca ttcgggaagt ttctccttgg ggtctggcac agaagcctcg
      421 ctcagcgagt gcatgtggtt gcgaagccta gatgaaacct cgatccagaa tgcccgcgct
      481 acttcgaccg gaaaatgtgc gtctctcaga cttgacatgt cggcacttag attttatgat
      541 ggccctcgaa ttggtcgggg actattccga gtcctcgaaa gaccagaaag gagaaagcga
      601 atggcaatgg tcagatggga accatcccgg gaactggaag cttttcagac cgatatgaac
      661 cggttattcg acagcttctt tcgctcgggt tccaattcga ccgtgcggag gtgggcgccc
      721 gccaccgacc tgaccgagga cggaaacgat ctggttctcc ggctcgatct gccaggactg
      781 tccgacgagg acgtggcggt ggaagtcgag ggcaacgtgc tcaccgtctc gggggaacgc
      841 cgggacgagc gggagaccaa ggaaggcagc acctaccggc tggaacgctc gttcgggaag
      901 ttcagtcgct ccttccccct cccggaggga accgatcccg acgcgatcga gggcgggatc
      961 gacaaagggg tgctggaact gcgcatcccc aagccaaccg agaagcagcc ccgccgagtc
     1021 gaactgggc
//
LOCUS       DAOQMF010000788         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_143708_length_1027_cov_3.433128, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000788 DAOQMF010000000
VERSION     DAOQMF010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143708_length_1027_cov_3.433128"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLV77_11130"
     CDS             complement(<1..423)
                     /locus_tag="PLV77_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNC16446.1"
                     /translation="MDQTNRNMALCTAFAEELDRSGVRLAVISPGSRSTPLALAFFRQ
                     DGIETEIGLDERSASFYALGAAQASGTPVVLVCTSGTAAANFHPAVAEADLSSVPLIV
                     LTADRPPELRDIGAGQTIDQIKLYGGSVRWFAEVGNHAA"
     gene            632..799
                     /locus_tag="PLV77_11135"
     CDS             632..799
                     /locus_tag="PLV77_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16447.1"
                     /translation="MSHRSAFRLSLFATLFAIFGLTAQIASAATLRVDNDKQQCPAAA
                     YTSVQAAVDAG"
     gene            799..>1027
                     /locus_tag="PLV77_11140"
     CDS             799..>1027
                     /locus_tag="PLV77_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16448.1"
                     /translation="MGDTVIICDGTYSEGSGQVGSNAVTITKSLTIKGAGADKVTIRP
                     TRVDPARQIASASPDLRDGVGSIIAVNGAPAT"
BASE COUNT          196 a          339 c          318 g          174 t
ORIGIN      
        1 ggcggcgtgg ttgccgactt ccgcgaacca gcgaaccgaa ccgccgtaca gcttgatctg
       61 gtcgatcgtc tggccggctc cgatgtcccg cagttccggt ggccggtcgg cggtgaggac
      121 gatcaacggc accgaggaga ggtcggcctc ggccaccgcg gggtggaagt tggccgccgc
      181 ggtgcctgaa gtgcagacca gtacgaccgg cgtgccggag gcctgcgccg cgccgagggc
      241 ataaaaggaa gcggacctct cgtcgagacc gatctcggtt tcgatgccgt cctggcgaaa
      301 gaacgcaagg gcaagaggcg tcgacctgga cccgggagaa atcaccgcca gacgcacgcc
      361 gctgcggtcc agctcctccg caaaagccgt gcaaagcgcc atattccgat tggtctgatc
      421 caaggaatcc ctgagcctag aactcttcgg agaataaggc gctccggggt cggacttgga
      481 tttcgccgtt gcatgaggta acttgacccc tcgtacaagc gggaaggtgg agccttagcc
      541 cggggaacaa tcgtccgtag cccgggcgca atcatcattc tccaccaccc aattagcgtt
      601 acttcgtcca tagtccttag ggagggatct ggtgtcacac cgttcagctt tcaggctgtc
      661 gcttttcgca acgctcttcg cgattttcgg gctcaccgcc cagattgcca gcgcagccac
      721 cctgcgagtc gataacgaca agcagcaatg ccccgccgcc gcgtacacct cggtccaggc
      781 ggcagtcgat gccgggtgat gggtgacacg gtcatcatct gtgacggcac ctactccgaa
      841 ggttccggcc aggtcggcag caatgcggtc accatcacca agtcgctcac gatcaaggga
      901 gccggtgccg acaaggtcac catccggccg acccgggtcg acccggcccg ccagatcgct
      961 tccgcctctc ccgacctgcg ggacggagtc ggcagcatca tcgcggtcaa cggcgccccg
     1021 gccactc
//
LOCUS       DAOQMF010000789         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_144294_length_1025_cov_3.244330, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000789 DAOQMF010000000
VERSION     DAOQMF010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144294_length_1025_cov_3.244330"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..>1025)
                     /locus_tag="PLV77_11145"
     CDS             complement(50..>1025)
                     /locus_tag="PLV77_11145"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC16449.1"
                     /translation="QYYRRGVADLRTGAKPRLRMRFRIASVAKAFSGAVALNLVARGK
                     LSLHSTIGEVLPGMLPKAKQVTLGQALHHTGGLPEYIKSKGFLNEVGNDPGAFVPPTR
                     IISWVRGSGLTHRPGTKYEYSDTDNIVVGLMAQRVTGVRYAWQIRRLGRQIGGLTATS
                     LPRTLRMPEPYIHGYEIEAGQQPNDVTRLVNPSGAWASGGIVSTLPNLSRFFRAYVAG
                     RFFGQGAKARAIKRAQRHWIRGESQPAGPGNNWAGMGLFRYVTRCGVMYGHTGSFPGY
                     RAFAASSADGKRSIAFVANAQIVPGRGSQKVNNRIRRMQVAAVCRALR"
BASE COUNT          169 a          325 c          366 g          165 t
ORIGIN      
        1 accggcaacg gcttcgaact ctcggccgac aagtactcga ccttctaggt cagcgcaggg
       61 cgcggcaaac cgccgctacc tgcatgcggc ggatccggtt gttgaccttc tgcgagccgc
      121 ggccgggaac gatctgggcg ttggcgacga aggcgatcga gcgcttgccg tcagccgagg
      181 aggcggcgaa ggcacggtag ccggggaagg aaccggtgtg gccgtacatc acgccgcacc
      241 tggtgacgta acggaagagg cccattccgg cccagttgtt gcccggcccg gccggctgtg
      301 actcgccgcg gatccagtgc cgctgggccc gcttgatcgc cctcgctttc gcaccttggc
      361 cgaagaagcg gcccgcaaca taggcgcgga agaagcggct caggttgggc agggtagaga
      421 cgatgccgcc ggaagcccag gcgccggagg gattgacaag gcgggtgacg tcattcggct
      481 gctggcccgc ttcgatctcg tagccgtgga tgtacggttc cggcatccgc agggtgcgag
      541 gcagggaggt ggcggtgagc cccccgatct ggcggccaag ccggcggatc tgccaggcgt
      601 agcggacgcc ggtcacccgc tgggccatca ggccgaccac gatgttgtcg gtgtccgagt
      661 actcgtactt cgtccccggg cggtgggtca gccccgatcc acggacccag gagatgatcc
      721 gggtcggcgg gacgaatgcc ccgggatcat tgccgacctc gttgagaaaa cccttcgact
      781 tgatgtattc gggcaggccg ccggtgtggt gaagcgcctg gccgagagtc acctgcttcg
      841 ccttcggcag catgccgggg aggacttcgc caatcgttga atgcaggctg agcttgccgc
      901 gagcgaccag gttcagcgcg accgcaccgc tgaaggcctt tgccacgctg gcgatccgga
      961 agcgcatgcg cagccggggc ttcgcaccgg tcctcaggtc ggcgacgcct cgacggtagt
     1021 actgc
//
LOCUS       DAOQMF010000790         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_144861_length_1023_cov_3.654959, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000790 DAOQMF010000000
VERSION     DAOQMF010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144861_length_1023_cov_3.654959"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..>1023)
                     /locus_tag="PLV77_11150"
     CDS             complement(202..>1023)
                     /locus_tag="PLV77_11150"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC16450.1"
                     /translation="LLAIVAVAGQLGNFSSLGEVPLWQLAGGLIGATYVALAATTISR
                     IGAGVIAAVTVTGQLACSVVVDHFGWFGIEVHDVTGPRILGLVLLLAGAMAMLYSREE
                     AGGGGASRAGRQTGHGIGSMASGAENRERLVICAVIFLASLAVGIQHPLNSELANSIG
                     DLPAGLTNFTVGTILLALLVIVTGQAKALLGAREARPQYFLGGLIGLIVVVTSLSAVT
                     LIGAAGLTAALVTGQMIGSLLLDRFGAFGLTRIPIDRVRLAAAAALLIGTFLATG"
BASE COUNT          190 a          353 c          347 g          133 t
ORIGIN      
        1 ggatgatttc catgagcgaa ccgaagcgct tgccgttgac catcgcgccg tcttcatcgg
       61 cgaggaagaa ggcccggtcg ccggggactc cgtcgcaggt caactcgacc tcgttcagcg
      121 actggatgcg catccccttg accggggcga tcgatatcca ggagacggtt ccggccacgc
      181 ccggatcctc tcatgcccgg atcagccggt ggcgaggaag gtgccgatca gcagggccgc
      241 cgccgcggcc agccggacgc ggtcgatcgg gatgcgggtc aggccgaagg cgccaaaccg
      301 gtcgagcagc agcgagccga tcatctggcc ggtgaccagg gccgcggtca ggccggccgc
      361 accgatcaag gtcacggctg aaagcgaagt gacgaccacg atcaggccga tcaggccgcc
      421 aaggaagtac tgcggccggg cctcgcgggc cccgagcagc gcctttgcct gcccggtgac
      481 gatcaccagc aacgccagca ggatcgtccc gaccgtgaag ttggtcagcc cggcgggcag
      541 atcgccgatc gaattcgcca gctcggagtt gagcggatgc tggattccga ccgccaggga
      601 ggcgaggaag atcaccgcgc agatgaccag acgctcgcgg ttctcggcgc cgctggccat
      661 cgacccgatc ccgtgacccg tctgccggcc ggcccgggaa gcccctccgc cgcctgcctc
      721 ctcccggctg tagagcatcg ccattgcccc ggccaggagc agcacgagac cgaggattcg
      781 cggcccggtc acgtcgtgga cttcgatccc gaaccacccg aagtgatcga ccaccaccga
      841 acaggcgagt tgaccggtaa ccgtgaccgc ggcgattacg ccggcaccga tccgcgagat
      901 cgtggtcgcc gcgagggcca cataggtggc accgatgagc ccgccggcca gctgccagag
      961 cggtacctca ccgagggaag agaagttgcc gagctggccg gcgaccgcga cgatggccag
     1021 cag
//
LOCUS       DAOQMF010000791         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_145294_length_1022_cov_2.067218, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000791 DAOQMF010000000
VERSION     DAOQMF010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145294_length_1022_cov_2.067218"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLV77_11155"
     CDS             <1..285
                     /locus_tag="PLV77_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16451.1"
                     /translation="PDDDGVGPNLRLDPSRTKALNHDPLEHLIEYPVYVRSIEEKGRW
                     DAAAKTATAMVSGENPLIEGNAETVWLATRALYKSDIPTETLETDLPEPR"
     gene            303..>1022
                     /locus_tag="PLV77_11160"
     CDS             303..>1022
                     /locus_tag="PLV77_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16452.1"
                     /translation="MRRYSSTAVAVSGEARESTGLRLGDILQNATGADSFRTDVPERI
                     GVLVKLDGAESTQRISLQRRGDEMVLVTWPGELKGQAGAFYETDRAGRVLGLIDTNGG
                     WRARSDFHLGFHTANKISQRFHPGEATEIHQYVARWSGPDADTLRAWKRDRVEDDLWG
                     WMLERDLVSERDLPAFEVYLGQLLNRDAQVRSGLELNRVWSWEDAVSLDEDGQLVAEV
                     REAIRMMLETLKEPMVPSLRGQ"
BASE COUNT          234 a          274 c          326 g          188 t
ORIGIN      
        1 ccggatgacg atggggtggg acctaatctg cgactcgatc cgtctcgaac caaggctctc
       61 aaccacgatc ccctcgaaca cttaatcgag tatccggttt acgtcagatc gattgaggag
      121 aaagggcgct gggatgctgc ggcgaagacc gcgacagcga tggtcagcgg cgagaacccg
      181 ttgattgaag gcaatgccga aacagtctgg ttggctaccc gggccctcta taagagtgac
      241 atcccaaccg aaaccctgga aactgatcta cccgaaccca gatgacatct tcaactggaa
      301 ctttgcgtcg ttactcaagc actgcggtcg cagtgtcggg tgaagcccgg gaatcaaccg
      361 gtctcaggct aggcgacatt cttcagaacg cgaccggtgc cgattcgttc cggaccgatg
      421 ttcccgagag gatcggggta ctcgtcaagc tggatggagc cgagtcaacc cagcgaatct
      481 cccttcagcg aaggggtgac gagatggtgc tggtcacctg gcccggagaa ctgaaagggc
      541 aggcgggggc cttttacgag acagaccgag ccggtcgcgt tctaggcctg atcgacacga
      601 acggcggatg gagagcacga agcgacttcc atctcggctt ccacacggca aacaagatct
      661 ctcaacggtt ccatcccggc gaggcgaccg aaatacatca atacgtcgcg aggtggtcag
      721 gcccggacgc cgacacactc agagcgtgga agcgagacag ggttgaggat gacctctggg
      781 gctggatgtt ggagcgtgac ttggttagtg agcgggattt accagccttc gaggtctatc
      841 tcggccaact gctgaatcga gacgcccagg ttcgttccgg cctcgaattg aatcgagtct
      901 ggagttggga ggatgcggtg agcctggacg aggacgggca gctggttgcc gaagtcaggg
      961 aagcgatcag gatgatgctc gaaacgctga aggagccgat ggtcccttcg ctgcggggcc
     1021 aa
//
LOCUS       DAOQMF010000792         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_146103_length_1019_cov_2.581950, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000792 DAOQMF010000000
VERSION     DAOQMF010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146103_length_1019_cov_2.581950"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..890)
                     /locus_tag="PLV77_11165"
     CDS             complement(291..890)
                     /locus_tag="PLV77_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16453.1"
                     /translation="MSGAPTTVTPEAPKMGARQRAMVLGIAAILVGVAVWGLTACNRD
                     GDPSSASEGVSMANTGDARIVSDDELTGLPEEVGHSLFWAGPKSGTQVEFSSDGTGNV
                     HLRYLTDGAEAGDPNQSYLNIGSYPFADAYEATRKLANGPDVVKVTEGEGTGFYDPNN
                     PYSVIIAWPSEPDLQVEVYDPVKNRALEVVRSGDIVPVS"
BASE COUNT          186 a          322 c          343 g          168 t
ORIGIN      
        1 gacccattcg gagcggggcc tcaagaaggc gaggctgcga accggcccgc gggggccgcc
       61 cgtgaagagg gccaggtaac cggcgacggc gagtccggtc acggcgcctg tggtggcctc
      121 ggcgagggcg gggaaacagg tgatgatgcc cccgaccgcg attacgcccg cgaagccggt
      181 tgccggcagg agcgtccacg gcagggttcg cccgcgggcg agttcggcca gcaggccgag
      241 gccgacgctg agcgccagca ggacgagagg gaagatgacc caggcgagaa tcaggacaca
      301 ggcacgatat cgccactgcg gacgacttcg agggcccggt tcttgaccgg gtcatacacc
      361 tcgacctgga ggtcgggctc gctcggccag gcgatgatca cgctgtaagg attgttcggg
      421 tcgtaaaatc cggttccttc accttcggtg accttgacca catccggtcc gttggcgagc
      481 ttccgggtcg cctcataagc gtcggcgaag gggtagctgc cgatgttgag gtagctctgg
      541 ttcgggtcac cggcttcggc cccgtcggtc aggtaacgga ggtggacgtt gccggttccg
      601 tcggagctga actcgacctg ggtgcccgac ttcggtcccg cccagaacaa actgtgaccc
      661 acctcttccg gcaggccggt gagttcgtca tcgctgacga tccgggcatc accggtgttg
      721 gccatagaaa cgccctccga ggccgacgac gggtcgccgt cccgattgca ggcggtcagc
      781 ccccagaccg cgactccgac caggatcgcc gcaattccga gcaccattgc tcgttggcgg
      841 gcgcccatct tgggcgcttc aggcgtgact gtcgtcgggg ctccgctcac agaagttgcg
      901 aagactagac cagcttcctt taggaaacct caaaagcgcg aggacgcaaa tcgagagccc
      961 ggaagcggat tcgaaccgct gaccccttcc ttaccatgga agtgctctac ctactgagc
//
LOCUS       DAOQMF010000793         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_147200_length_1015_cov_3.280208, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000793 DAOQMF010000000
VERSION     DAOQMF010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147200_length_1015_cov_3.280208"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLV77_11170"
     CDS             <1..313
                     /locus_tag="PLV77_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC16454.1"
                     /translation="EVTSGKGMISRQSLSALVRMPLVATPWWKSFGVMNENKGVFGLN
                     LLDWWKDEGLVRLGALLEAGLERGDFTPVVDRSFGFEEAGDAHRFIADRQNVGKVLLE
                     P"
     gene            515..>1015
                     /locus_tag="PLV77_11175"
     CDS             515..>1015
                     /locus_tag="PLV77_11175"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935028.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNC16455.1"
                     /translation="MSSQDTDVNRVQIFDTTLRDGEQSPGISLNANEKIEIAQQLARL
                     GVDVIEAGFPIASPGDFEAVQRIAREVHGPTICGLARAQAPDIDAAWNAIRDSEQPRI
                     HTFISTSDIHIQHQMRNTREDVLKGARAAVAQARSYCEDVEFSPMDATRSDVEFTAEV
                     CAIAVEE"
BASE COUNT          188 a          317 c          323 g          187 t
ORIGIN      
        1 cgaagtgacc tccggcaagg ggatgatcag ccggcagtcg ctctcggctc tggtcaggat
       61 gccgctcgtg gccacgccct ggtggaagag cttcggggtg atgaacgaga acaagggcgt
      121 cttcggtctc aacctgctcg actggtggaa ggacgagggc ctggtcaggc tgggggccct
      181 gctcgaggct ggactggagc ggggagactt caccccggtg gtcgaccgct ccttcggttt
      241 tgaggaagcc ggtgatgctc accgcttcat cgccgaccgc cagaacgtcg gcaaggttct
      301 gctcgagcct tgaaacctgc cagtttcctt gccagggccg gttccttgaa gtcgccccgc
      361 tccttgcgct acgcttgacc ctcggtatga atcaccgttt caacagcccg gcccttcttc
      421 tccttctccc cctctggggg gactgatcgc cgggcggcgc gatagccgcg agctgaaagc
      481 aagattcgag ttacttagct agaaggagaa ggacatgtcc agtcaggaca cagacgtaaa
      541 ccgcgttcag atatttgaca cgaccctgag agacggggag cagtctccgg gaatctcgtt
      601 gaacgcgaac gagaagatcg agatcgccca gcagctggcg cggctcggcg tagacgtgat
      661 cgaagccggc ttcccgatcg cttcgcccgg tgacttcgag gcggtccagc ggatcgcccg
      721 tgaggttcac ggcccgacca tctgtggcct ggctcgcgcc caggccccgg acatcgacgc
      781 cgcctggaac gcgatccgcg attccgagca gccccggatc cacaccttca tttccacctc
      841 tgacatccac atccagcatc agatgcggaa caccagggag gacgtgctga agggagcccg
      901 ggccgccgtc gcccaggcca ggtcctactg tgaggatgtc gaattctcgc cgatggatgc
      961 gacccgcagt gatgtcgaat tcacggccga ggtctgtgcg atcgcggtcg aggag
//
LOCUS       DAOQMF010000794         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_147233_length_1015_cov_2.877083, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000794 DAOQMF010000000
VERSION     DAOQMF010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147233_length_1015_cov_2.877083"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLV77_11180"
     CDS             complement(<1..303)
                     /locus_tag="PLV77_11180"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNC16456.1"
                     /translation="MSEETQQPATLSSVEELFEEIDVEQPQVGIIMGSKNDKPKMQPA
                     GQALEDAGIAYEVRVMSAHRDPVLVAEYCTNAKMRGLKVIIAGAGMSAALPGVAAAH"
     gene            complement(300..>1015)
                     /locus_tag="PLV77_11185"
     CDS             complement(300..>1015)
                     /locus_tag="PLV77_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HNC16457.1"
                     /translation="LSLLAICDGERAIPLAPAQDYKRIGEGDTGPNTGGMGSYSPVQT
                     VSKDQLDELSALVHQPIVDLMARRGHPFHGVLYAGLMMTADGPKVLEYNTRFGDPETQ
                     AVLPRLESDLVDLFLSASEPGGLAGTEVSFADQFAVTVVLASAGYPASSSKGDVITGL
                     DEAARSAEVVHAGTAEADGRIVTAGGRVLNLTGTGPDAATARDRAYDAADAVNFDGKQ
                     MRRDIAERAVRRERNQKAT"
BASE COUNT          151 a          332 c          340 g          192 t
ORIGIN      
        1 gtgggcggcg gcgactccgg gcagggcggc cgacatgccg gccccggcga tgatcacctt
       61 gaggccgcgc atcttcgcgt tggtgcagta ctcggcgacc agtacggggt cgcgatgggc
      121 cgacatgacc cgcacctcgt aggcgatgcc ggcgtcctca agggcctgtc cggccggctg
      181 cattttgggc ttgtcgttct tcgagcccat gatgatcccc acctggggct gttcaacgtc
      241 gatttcctcg aagagctctt cgaccgagga aagggttgcc ggctgctggg tttcttcgct
      301 catgttgctt tctggtttcg ttcgcgccgg acagcccttt cggcgatgtc gcgtcgcatc
      361 tgttttccat cgaaattgac tgcgtcagcc gcatcgtaag cgcgatcgcg cgcggttgca
      421 gcatcgggtc ccgtaccggt gaggttcagc acccggccac cggcggtgac gatccgtccg
      481 tccgcttccg ccgtgccggc gtgaaccact tccgccgacc gtgccgcttc gtcgagtccg
      541 gtgatcacgt cacccttcga ggacgaggcg gggtaaccgg ccgaggccag caccacggtc
      601 accgcgaact gatcggcaaa gctgacctcg gttccggcca ggcctcccgg ctccgaggcg
      661 gaaaggaaga ggtcgaccag gtcggactca agccgcggga gtaccgcctg ggtctccggg
      721 tcgccaaagc gggtgttgta ctcgagaacc ttcggaccgt cggcggtcat catcaggccc
      781 gcatagagca ccccgtggaa ggggtggccg cggcgggcca tcaggtcgac gatcggctga
      841 tgcaccaggg ccgacagttc gtccagttga tccttgctga cggtctggac cggcgagtag
      901 ctgcccatgc cgccggtgtt cgggccggtg tcaccctcac cgatccgctt gtaatcctgg
      961 gccggggcca gggggatcgc cctctcgccg tcgcagatcg caagcagcga gagct
//
LOCUS       DAOQMF010000795         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_147471_length_1014_cov_3.711157, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000795 DAOQMF010000000
VERSION     DAOQMF010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147471_length_1014_cov_3.711157"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..285
                     /locus_tag="PLV77_11190"
     CDS             55..285
                     /locus_tag="PLV77_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNC16458.1"
                     /translation="MSRLLSVSLPDELADEIESLVEASGKNKSELVRDALRRQIRLER
                     VYSVRRLGQSRAETAGIGPEDVEDLIDRFRKE"
     gene            263..703
                     /locus_tag="PLV77_11195"
     CDS             263..703
                     /locus_tag="PLV77_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNC16459.1"
                     /translation="MTGSAKSDSSTRIACDTNVMVSAFIAGGPPGRVIEEAADGSLGL
                     VLLEPVLNELTRILPAKFDFDTAELRQLLDLLADISVDAQGPPAGPPEPVTGDPDDDL
                     ILACAVESSATVLVSGDRRHLLPVDRHRGIRILTPQALLAELRS"
     gene            complement(736..>1014)
                     /locus_tag="PLV77_11200"
     CDS             complement(736..>1014)
                     /locus_tag="PLV77_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC16460.1"
                     /translation="MLELPGVEERKERCDLLIALGHRRDELDIPTITGFAGHEDRVVR
                     YVVLAMLSWNPSIANDYLAGKFSDDPDPLVRELARRWSGGRLRRRTRA"
BASE COUNT          179 a          331 c          322 g          182 t
ORIGIN      
        1 ggcgtcaacc ccggggcccg gaagctcatg aacccgttga tatgatgatt tcatatgagt
       61 cgtctgcttt cagtttcact ccccgatgag cttgccgacg agatcgaatc gctggtcgaa
      121 gcttccggca agaacaagtc cgaacttgtt cgcgacgccc tccggcgaca gatcaggctg
      181 gaacgggtct actcggtccg gcgcctcggg caaagccgcg ccgagacggc cggaatcgga
      241 cccgaggatg tcgaggatct gattgaccgg ttccgcaaag agtgattctt caacaaggat
      301 cgcctgtgac accaacgtga tggtttcggc cttcatcgcg ggcggcccgc ccggccgggt
      361 gatcgaggaa gcagctgacg gaagcctcgg gcttgtcctg cttgaaccgg tcctgaacga
      421 attgacacga atcctcccgg caaagttcga cttcgatacc gctgaactgc gccagttgct
      481 cgatctgctc gctgacatct cggtggacgc acagggaccg ccagccgggc ctccggagcc
      541 ggtcaccggt gacccggacg atgacctgat cctcgcctgc gccgtcgaat cgtcggccac
      601 cgtgctcgtc tccggggacc ggcggcacct gctgccggtg gatcggcacc ggggaatccg
      661 gatcctcaca ccgcaggctt tgctggcaga actcaggtcc tgacccgcgc ttccgggtca
      721 ggctcgggtt ccggctcagg cgcgggttcg gcggcgcaac ctgccaccgg accagcgccg
      781 ggcaagttcc cggacgagcg gatcggggtc gtcgctgaac ttgcccgcca gatagtcgtt
      841 ggcgatcgaa gggttccagg acagcatcgc gagcacgacg tagcggacca cccggtcttc
      901 gtgcccggcg aacccggtga tcgtcgggat gtcaagctcg tcccggcgat gacccaaggc
      961 gatcagcagg tcgcagcgtt ccttccgttc ctccacgccg ggcagctcga gcat
//
LOCUS       DAOQMF010000796         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_148394_length_1011_cov_3.497908, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000796 DAOQMF010000000
VERSION     DAOQMF010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148394_length_1011_cov_3.497908"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLV77_11205"
     CDS             complement(<1..363)
                     /locus_tag="PLV77_11205"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HNC16461.1"
                     /translation="MQANLKELEDRLWDAADDLRANSGLKASEYGTPVLGLIFLRFAD
                     VRFEAESPRIEAKGSARRKIGPADYQAERVIYLAPAARFGALLDLPEGSDLGKAVNEA
                     MRLVEEHNPDLAGVLPRNY"
     gene            complement(610..>1011)
                     /locus_tag="PLV77_11210"
     CDS             complement(610..>1011)
                     /locus_tag="PLV77_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16462.1"
                     /translation="LLILLVASLLDGLKLPRWLPAALLPLALISLGLNVSQFSDGRDF
                     LLKQTEITRAAIGQVEIEDPVPNFSLVEEATGSPFQRLIETDAYLRAEKRFGSPAYSP
                     AQIRASGPAGREAAGGVAFAAALARKLAHDK"
BASE COUNT          179 a          314 c          334 g          184 t
ORIGIN      
        1 gtagttgcgc ggcagaactc cggccagatc ggggttatgc tcctcgacca gtcgcatcgc
       61 ttcattgact gccttaccaa ggtcggaacc ttccgggaga tcgagcaatg ctccaaaacg
      121 agccgccgga gcgaggtaga tgacccgttc agcctggtaa tcagccgggc cgatcttccg
      181 ccgggcggag cccttggctt cgatcctcgg actctcggct tcaaagcgaa catcggcgaa
      241 gcgcaggaag atcagaccga gaaccggcgt gccatattcg gaagccttca ggccagagtt
      301 tgcacggagg tcatccgccg cgtcccacag ccgatcctca agctccttca gatttgcctg
      361 cacctacgac ctcccggttg gtggaatggc ttggcggcgc aagcctagcc cggaacttct
      421 tgcgcaagtc gtgcgcctct cctagctgaa gaaatctcca cagccttgtc acgcggggag
      481 taggtttctc ggcgggctcc ttgaggcgcc ggcaagcttg gaactgctcc tcgagatcgg
      541 tgaggcgtga cttcttcgtc gttatgacga agaagccgga tctcgccgaa cgggagcgtg
      601 gctctcgggc tatttgtcgt gggcgagctt gcgggccagt gcggcggcga aggcgacgcc
      661 cccggcggcc tcccggccgg ccgggcccga agcccggatc tgcgccgggc tgtaggccgg
      721 tgagccgaag cgtttctctg cccgcaggta ggcgtcggtc tcgatcagtc gctggaacgg
      781 gctcccggtt gcttcctcga ccaggctgaa gttgggaacc gggtcctcga tctcgacctg
      841 gccgatcgcc gcccgggtga tttcagtctg cttgagcagg aagtcccgcc cgtcactgaa
      901 ttgcgacacg ttcaggccga gcgaaatgag ggccaggggg agcagggccg cgggcagcca
      961 gcgcggaagc ttcagaccgt caagcaggct ggcgacgagc aggatcagca g
//
LOCUS       DAOQMF010000797         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_148659_length_1010_cov_4.052356, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000797 DAOQMF010000000
VERSION     DAOQMF010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148659_length_1010_cov_4.052356"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLV77_11215"
     CDS             <1..782
                     /locus_tag="PLV77_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HNC16463.1"
                     /translation="VTRGAPAATLDLSDLRREVDGAMQEATSALAQITDLLALVVAPP
                     LGTARIHRVEALLLQPEVVMVIVITSTGDVTRRAFTFASPVDPGLVEWASSYLNESLS
                     GIDLGARRIASKLADPALGPLEANFIEQLAPALVDLEQGLEGTLYVDGASRLLDQRRA
                     ADLPRIDRLMTTLEEKATVLRMMRSAIDERSVFLWIGAENPQPELQDVTVIGANYGLG
                     HRNLGSVGVVGPTRMDYEKAIASVSEAARELSRYFEFVYDA"
     gene            779..>1010
                     /locus_tag="PLV77_11220"
     CDS             779..>1010
                     /locus_tag="PLV77_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNC16464.1"
                     /translation="MSEKRDYYEILGVSREATDAEIKKAFRKVARELHPDVNAHDPEA
                     EEKFKEAAEAYEVLSDAERRGTYDQFGHDGLRS"
BASE COUNT          174 a          346 c          331 g          159 t
ORIGIN      
        1 cggtaacccg cggtgcgccc gcggccaccc tcgacctcag cgacctgcgt cgcgaggtcg
       61 acggggcgat gcaggaagcg acctcggccc tggcccagat caccgacctg ctcgccctgg
      121 tcgtggctcc gccactgggg acggcccgga tccaccgggt cgaagccctg ctgctgcagc
      181 ccgaggtggt gatggtgatc gtgatcacat cgaccggtga cgtcacccgg cgggccttca
      241 ccttcgcctc gccagtcgat cccggtctgg tcgagtgggc ctccagctac ctgaacgagt
      301 cgctctccgg catcgatctc ggggctcgcc ggatcgcctc gaaacttgcc gatccggcgc
      361 tcggtccgct tgaggccaat ttcatcgagc agcttgcgcc ggccctggtc gacctcgaac
      421 agggcctcga gggcacgctc tacgtcgatg gtgcctcccg gctgctcgat cagcgccggg
      481 ctgccgacct gccgcggatc gaccgcctga tgaccacact ggaggagaag gcgaccgtgc
      541 tcaggatgat gcgctcggca atcgacgagc gctccgtctt cctctggatc ggggccgaga
      601 atccccagcc cgaactgcag gacgtgaccg tgatcggcgc caattacggg ctcggccacc
      661 ggaacctggg ctcggtcggg gtggtcggcc cgacccgcat ggattacgag aaggcgatcg
      721 ccagcgtcag tgaagccgcc cgcgagcttt cccgctactt cgaattcgtc tacgacgcat
      781 gagcgaaaag cgcgattact acgagattct cggggtaagc cgggaagcga ccgacgcgga
      841 gatcaagaag gcattccgca aggtcgcccg ggaactccac ccggacgtga acgctcacga
      901 tcccgaggcc gaagagaagt tcaaggaggc ggccgaggcc tacgaggtgc tctccgacgc
      961 cgagcggcgg ggaacctacg accagttcgg tcacgacggg cttcgctccg
//
LOCUS       DAOQMF010000798         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_148692_length_1010_cov_3.375916, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000798 DAOQMF010000000
VERSION     DAOQMF010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148692_length_1010_cov_3.375916"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLV77_11225"
     CDS             <1..899
                     /locus_tag="PLV77_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HNC16465.1"
                     /translation="AWVEGEATNPAYEVEVVAEEAAPLTGRTSGYAILPAKTIDRCRG
                     SIDTLIVAGGTGVLEAERSEPLVRWIGKAAGRSRRVASVCSGSLLLAATGLLDGHRAT
                     SHWASCSELSRRYPEVEVDPDPIFVQDGRIWTSAGVTAGMDLSLALVAEDLGEEVALE
                     VARWLVLFLRRPGGQAQFSSHVSAPASRPRLRELQLWMADHLDSDLRVETLADRAMMS
                     PRNFARAFRRETGMTPGTYVETLRLERARQLLEGPPGPIDGIAVQCGFGTPETMRRSF
                     ARRLGVSPAEYRRRFRREPAEV"
BASE COUNT          157 a          349 c          333 g          171 t
ORIGIN      
        1 cagcctgggt cgaaggtgaa gcgactaacc cggcctacga ggtggaagtc gtggcggaag
       61 aggcggcgcc cctgaccggt cggaccagcg gctacgcgat ccttcccgcg aagacgatcg
      121 accgctgccg ggggtcgatc gacacgctga tcgtcgccgg cgggaccggt gtgctggagg
      181 ccgaaaggtc cgagccgctg gtgcggtgga tcgggaaggc ggccggccgg tcccgccggg
      241 tcgcttcggt ctgcagcggt tcactgcttt tggcggccac cggtctgctc gacggccatc
      301 gggcgaccag tcactgggcg tcctgttccg agctttcccg tcgctacccg gaggtggagg
      361 tggaccccga tccgatcttc gtccaggacg gacgcatctg gacctcggcc ggggtcacgg
      421 ccgggatgga cctatctctc gccctggtcg ccgaggacct cggcgaggaa gtggcgctcg
      481 aggtggcccg ctggctggtg ctctttctcc gtcgacccgg aggccaggcc cagttcagtt
      541 cgcatgtctc ggctcccgct tcccggcccc gccttcgcga gctccagctc tggatggccg
      601 accatctcga ttccgatctt cgagtcgaga ccctggccga ccgggcgatg atgagccccc
      661 gcaactttgc ccgcgcgttc cgtcgggaaa ccgggatgac gccgggcaca tacgtcgaga
      721 cgctgagact cgaaagggcc cggcagctgc tcgaaggacc acccggcccg atcgacggga
      781 tagccgtcca gtgcggtttc ggaacccccg aaaccatgcg ccggtctttc gcccgccggc
      841 tgggcgtttc acccgccgaa taccgccgtc gattccgccg cgaaccggcg gaagtctgaa
      901 cccgacccaa ggagaaccat gcaagtcgca tacctgctct acgaccgttt caccgccctc
      961 gacatcactg gcccacacga ggtcttcaat tcggtgccgg atgtcgaatc
//
LOCUS       DAOQMF010000799         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_148938_length_1009_cov_4.396226, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000799 DAOQMF010000000
VERSION     DAOQMF010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148938_length_1009_cov_4.396226"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLV77_11230"
     CDS             <1..337
                     /locus_tag="PLV77_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC16466.1"
                     /translation="GPLRAWHDASRTEIGQLLLSMRGTPELLEFSDERLAPLLDLPGE
                     RGRDLLDTLEVYLHSGGRKTEAATELEIERQSLYHRLSRIEEVLDVDLKDGDTVLALH
                     LAIMINRLL"
     gene            complement(347..937)
                     /locus_tag="PLV77_11235"
     CDS             complement(347..937)
                     /locus_tag="PLV77_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16467.1"
                     /translation="MSDLTGQPELSDRLSQIPKNEPFPVVGLVIFFVGMVMVAYGITA
                     LWFGMREVMDVGGYCAEGGPYVVRQHCPDGAEMLMFTGIPIGIIGLFIALLGGAKSAK
                     GAAGLLLLGWPALFISLGYNFIDYAINPPEGMGSTAGWWVCGILFALMGLPALAGIPM
                     LVKAIQPERRNAVLAVFLIAAAAGVVIGIQVANAAD"
BASE COUNT          185 a          340 c          332 g          152 t
ORIGIN      
        1 cgggccgctg cgggcctggc acgacgcgtc acggaccgag atcgggcagt tgctgctttc
       61 gatgaggggc acgccggaac tgcttgagtt cagcgacgag cggctcgcgc ccctgctcga
      121 cctgccgggg gaaaggggtc gtgacctgct cgacacgctc gaggtctacc tgcacagtgg
      181 cggacggaag accgaggccg cgaccgaact ggagatcgag cggcagtcgc tctatcaccg
      241 gctctcacgc atcgaagagg tgcttgacgt ggacctgaag gatggcgaca cggtgctcgc
      301 cctccacctc gcgatcatga tcaaccgcct gctctagggc gggcttctag tcagccgcgt
      361 tcgcgacctg gatcccgatc acgaccccgg ccgccgccgc gatcaggaag acggccagga
      421 cggcgtttcg cctttccggc tgaatcgcct tgaccagcat cggtatcccg gccagggcag
      481 gcagtcccat cagggcgaaa agtatcccgc agacccacca gccggccgtg ctgcccatcc
      541 cctccggcgg gttgatcgcg tagtcgatga agttgtagcc gagcgagatg aagagggcgg
      601 gccagcccag gagcagcagc ccggccgccc ccttcgccga cttggcgcct cccagcaagg
      661 cgatgaacag gccgatgatc ccgatcggaa tgccggtgaa catcagcatt tcggctccgt
      721 ccgggcaatg ctggcggacc acgtacggcc cgccctcggc gcagtagccg ccgacgtcca
      781 tcacttcgcg catcccgaac cagagggcgg tgatgccgta ggcgaccatc accatcccga
      841 cgaagaagat gaccaacccg accaccggga agggctcgtt cttcgggatt tgcgaaagcc
      901 ggtcggagag ctccggctgg ccggtcaggt cggacacggg cggaagctta gctggccgct
      961 acctgggcct ggagctccgg aacgtcgaca gcgcgtcgct cgtcaacga
//
LOCUS       DAOQMF010000800         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_150177_length_1005_cov_3.480000, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000800 DAOQMF010000000
VERSION     DAOQMF010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150177_length_1005_cov_3.480000"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLV77_11240"
     CDS             complement(<1..375)
                     /locus_tag="PLV77_11240"
                     /inference="COORDINATES: protein motif:HMM:NF037142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HNC16468.1"
                     /translation="MSTDNAVDALSDREHLVELARGKPTPPYTLDEELTFFCPQENLE
                     GLDLPVVREFHRWVTTEHQPAPGDEKAVLLLLPCEKAKPYSLSAEHRAVSNALGEAGY
                     EPRERGDWPEELAGLASPQELSN"
     gene            complement(372..>1005)
                     /locus_tag="PLV77_11245"
     CDS             complement(372..>1005)
                     /locus_tag="PLV77_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16469.1"
                     /translation="LIESLGAERVAIVDWDAHHGNGAQGIFWDDPRVLTVSIHQDGAF
                     PPGSGSWDETGGPGAAGTNLNLPMPPGTGVGGYSAAFERVIVPALERFAPDFILVACG
                     FDPGPQDPLARLMVHSDGFREMTARVRECAERICDGRLALFHEGGYSRAYVPFCGLAV
                     VEALSGIDSGVQDPFLKGYAGFPYQSLQPLQESVIERAETQIGETRKGDE"
BASE COUNT          148 a          347 c          355 g          155 t
ORIGIN      
        1 gttgctcagc tcctgcgggc tcgccagacc ggcaagctcc tccggccagt ccccgcgctc
       61 gcgcggttcg tagccggcct cgcccagcgc attgctgacc gcccggtgct cggccgagag
      121 cgagtagggc ttggccttct cgcaggggag cagcagcagg accgccttct cgtcccccgg
      181 ggccggttgg tgttcggtcg tgacccagcg gtggaactcc cgcaccaccg gcaggtcgag
      241 gccttcgagg ttctcctgcg ggcagaaaaa agtcagttcc tcgtcgaggg tgtagggcgg
      301 cgtcggcttg ccccgcgcca gctcgaccag gtgttcgcgg tcgctcaggg catccaccgc
      361 gttatcagtg ctcattcatc gcctttccgg gtttccccga tctgagtttc ggcccgctcg
      421 atcaccgatt cctgcagggg ctgcagggac tggtagggaa agccggcgta tcccttaagg
      481 aaggggtcct ggaccccaga atcgatgccc gacagcgcct cgaccacggc caggccgcag
      541 aacggcacgt aggcccggga gtagccgcct tcgtgaaaga gggcgagtcg cccgtcgcag
      601 atccgctccg cgcactcgcg cacccgcgcg gtcatctcgc ggaagccgtc ggagtggacc
      661 atcagccggg cgagcggatc ctgggggccg gggtcgaagc cgcaggcgac caggatgaag
      721 tcgggggcga accgctccag ggccgggacg atgacccgct cgaaagcggc cgagtacccg
      781 ccgacgccgg tcccgggagg catcggcagg ttgaggttgg tgccggccgc tcccggaccg
      841 ccggtctcgt cccagctgcc cgagccgggc gggaaggcgc cgtcctgatg gatcgagacg
      901 gtcaggacgc gcgggtcgtc ccagaagatc ccctgggcgc cgttgccgtg atgggcgtcc
      961 cagtcgacga tcgcgacccg ctccgcgccg aggctctcga tcagg
//
LOCUS       DAOQMF010000801         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_150188_length_1005_cov_3.308421, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000801 DAOQMF010000000
VERSION     DAOQMF010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150188_length_1005_cov_3.308421"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLV77_11250"
     CDS             complement(<1..647)
                     /locus_tag="PLV77_11250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC16470.1"
                     /translation="MSERGKLVTLGVIGAVLAVVVLVVLVGRGGDDDDSSIATPVTKD
                     VKLSRPEPGSVVQPGEKASVKMETTEGDFTISLDTENWPVTANSFAYLTEEKFYDGLG
                     FHRIVPDFVIQGGDPTGTGSGGPGYSVVETPPKSTTYPVGTVAMAKTGAEAPGTSGSQ
                     FFIVTGAQGSSLTPDYSIAGTVSEGLEVVKRIGELGSASEQPTKQVVIEKATLEKG"
     gene            688..>1005
                     /locus_tag="PLV77_11255"
     CDS             688..>1005
                     /locus_tag="PLV77_11255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC16471.1"
                     /translation="MPEYRSAADRKTILVGKTICRLAAVAPWAWPLIGKSVGRFFDRA
                     AAGWDERTAAGSAGHLATLAAGLDSLEVKPERVLDLGCGTGAGTLFLAREFPRAGIRG
                     VDLS"
BASE COUNT          149 a          339 c          318 g          199 t
ORIGIN      
        1 cccttctcga gcgtcgcctt ttcgatgacg acctgcttgg tcggctgctc gctggccgag
       61 cccagttccc cgatccgctt gacaacctcg aggccttcgg acaccgtccc ggcgatcgag
      121 tagtccggtg taaggcttga tccctgagct ccggtcacga tgaagaactg gctgcccgag
      181 gttccgggcg cttcggctcc ggtcttggcc attgccaccg tgccaaccgg gtaggtggtg
      241 ctcttcggcg gagtttcgac cacgctgtaa ccggggccgc cggatccggt gccggtcggg
      301 tccccgccct ggatcacgaa gtcggggacg atccggtgga agccaagccc gtcgtagaac
      361 ttctcctcgg tcagataggc gaagctgttc gcggtgaccg gccagttctc cgtgtcgagg
      421 ctgatcgtga agtcaccctc ggtcgtctcc atcttcacgc tggccttctc gcccggctgg
      481 accactgagc ccggttcggg ccgggacagc ttgacgtcct tggttacggg ggtggcaatg
      541 ctcgaatcat cgtcgtcacc accgcgcccg accagcacca ccaggaccac gacggccagg
      601 acagcgccga tcacgccaag ggtgaccagc tttccgcgtt cactcatcgg cgccagtcta
      661 tggacttgcc tttgtaatga tcaggaggtg cccgagtacc gaagcgcagc cgaccgcaag
      721 acgatcctgg tcggcaagac catctgtcgc ctggcagcag ttgctccctg ggcctggccg
      781 ctgatcggaa agtcggtcgg tcgtttcttc gaccgcgccg ctgccggctg ggatgaacgc
      841 actgcggccg gttcggccgg gcatctggcc actctcgccg ccgggctcga ctcactcgag
      901 gtgaaaccgg aacgggtgct tgatctcggc tgcggaaccg gcgcggggac gctctttctc
      961 gcccgcgagt ttccccgggc cggaattcgc ggtgtcgacc tttcc
//
LOCUS       DAOQMF010000802         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_150330_length_1005_cov_2.035789, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000802 DAOQMF010000000
VERSION     DAOQMF010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150330_length_1005_cov_2.035789"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1005)
                     /gene="mltG"
                     /locus_tag="PLV77_11260"
     CDS             complement(<1..>1005)
                     /gene="mltG"
                     /locus_tag="PLV77_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNC16472.1"
                     /translation="RSGRSGGPRKGRIVAAAAGAVGLIIVIWFLFALFQPFKGDGDGE
                     PFPVKVPAGSSVGEIGDLLAGKGIISNATLFEIRATISGKRGDLNHGVHQFRENMSYS
                     AAIDELSKTTSKRTISITIPEGLSRSETAAVVKAAGLPGDYMAATVAVKGFDPDDYGA
                     QGRAENLEGFLFPATYELKPAADVEDLVAEQLQAFKDNIAGVNMKYAKSKNLTVYDVL
                     TIASMIEREVSVPSERKTVAAVIYNRLKMGEPLGIDATIRFALDNWTEPLTDADLETD
                     SPYNTRTNQGLPPGPIGNPGLASIEAAAHPADTDVWLYVVKPGTCGEHVFSSSLAEHN
                     R"
BASE COUNT          155 a          320 c          345 g          185 t
ORIGIN      
        1 gcggttgtgc tcggccaggc tcgaggagaa gacatgctcg ccgcaggtgc cgggcttgac
       61 cacatacagc cagacatcgg tgtcggccgg atgcgcggcc gcttcgattg aagcgaggcc
      121 gggattcccg atcggccccg gcggcaggcc ctggttggtg cgggtgttgt acggcgaatc
      181 agtctccaga tcggcgtcgg tcagcggctc ggtccagttg tcgagcgcga agcggatcgt
      241 cgcatcgatg cccagcggct cacccatctt gagccggttg tagatcaccg ccgcgaccgt
      301 cttgcgctcg ctcggaacgg aaacctcgcg ctcgatcatc gaggcgatgg tcagcacgtc
      361 gtagacggtc aggttcttcg acttcgcgta cttcatgttg accccggcga tgttgtcctt
      421 gaacgcctgg agttgctcgg cgacgagatc ctccacgtcg gcggccggct tcagctcgta
      481 ggtcgccggg aagaggaacc cttcgaggtt ttcggcccgg ccctgtgccc cgtagtcatc
      541 cgggtcgaaa ccctttaccg ccacggtggc ggccatgtag tcgccgggca ggcccgccgc
      601 cttgaccacg gcggcggttt cagagcgact gaggccctcg gggatcgtga tcgagatggt
      661 ccgcttcgag gtggtcttgc tcagttcgtc gatcgccgcc gagtacgaca tgttctcgcg
      721 gaactgatgg accccgtggt tgagatcgcc gcgcttgccg gagatcgtcg cccggatctc
      781 gaacagggtc gcgttggaga tgattccctt cccggcgagc aggtcaccga tctcgccgac
      841 gctgctgccg gccggcacct tgaccgggaa tggttcgccg tcaccgtcgc ccttgaacgg
      901 ctggaagagg gcaaagagga accagatgac gatgatcagg ccgaccgcgc cggccgcagc
      961 ggccacgatc cggcccttgc gggggccgcc ggagcggccg gagcg
//
LOCUS       DAOQMF010000803         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_150845_length_1003_cov_3.117089, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000803 DAOQMF010000000
VERSION     DAOQMF010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150845_length_1003_cov_3.117089"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLV77_11265"
     CDS             complement(<1..977)
                     /locus_tag="PLV77_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNC16473.1"
                     /translation="MGRYSHVKAGFLPAPPIRLNAFIPDFLDKRLLFVTGKGGVGKTT
                     VAMALGIRAAMEGKRTIVCEVAATENASRLFAQSEVGFREVELANDLWAISIDPDESM
                     REYVLLQLKVKAMRDLLFRSRMFTYLAAATPGLNELVTIGKIWELAQFDRKVKHGRKY
                     DLVIVDAPATGHGISFLQTPRTFASIARVGPIHSQALQLEALITDHEQTGTVIVSLPE
                     EMPVNETARLESELSGDIGIAIDRVVMNALYPETFPKGDVKRLGKLKKSDDAEVRAAG
                     RAGASQATRAAAQREQLARLEEMVETEVTTLPFIFKPELDLKAARQLAE"
BASE COUNT          177 a          304 c          332 g          190 t
ORIGIN      
        1 tcctcggcca gctggcgggc cgccttgagg tcgagctccg gcttgaagat gaaggggagg
       61 gtggtgacct cggtctcgac catttcctcc agccgggcga gctgttcgcg ctgggccgcg
      121 gcccgggtgg cctgggaggc gccggccctg cccgcggcac ggacctcggc gtcatcgctc
      181 tttttcagct tgcccagccg tttcacatcg cccttcggga aggtttccgg gtaaagcgcg
      241 ttcatcacga cacggtcgat cgcgatgccg atgtcaccgg aaagctccga ttcgagccgg
      301 gcggtttcgt ttaccggcat ctcctccggc aaggagacga tcaccgtgcc ggtctgttca
      361 tggtcggtga tcagcgcctc cagctgcagg gcctggctgt ggatcgggcc gacccgggcg
      421 atcgaggcga aggtccgcgg ggtctgcagg aagctgatcc cgtggccggt cgccggggca
      481 tcgacgatga ccaggtcata cttgcggccg tgcttgacct tgcggtcgaa ctgggcaagc
      541 tcccagatct tgccgatcgt gaccaactcg ttcaggccag gggtggcggc cgcaaggtag
      601 gtaaacatgc ggctgcgaaa aagcaggtcc ctcattgcct tgactttcag ctgcagcagc
      661 acgtactcgc gcatcgactc gtccgggtca atcgagattg cccagaggtc gttggccagt
      721 tcgacttcac ggaagccgac ctcggactga gcaaaaagac gggaggcgtt ttcggtcgcc
      781 gcaacctcgc agacgatggt cctcttgcct tccatcgcgg cgcggatacc gagagccatg
      841 gcgaccgtgg ttttgcccac gccgcccttt ccggtgacga agagcaggcg tttgtcgaga
      901 aagtcgggaa tgaaggcatt caaccggatt gggggtgcgg ggaggaaacc ggctttcaca
      961 tgcgaataac ggcccaaatg cgaatcctga gccgcaagaa cca
//
LOCUS       DAOQMF010000804         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0529
            NODE_151468_length_1001_cov_3.189218, whole genome shotgun
            sequence.
ACCESSION   DAOQMF010000804 DAOQMF010000000
VERSION     DAOQMF010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810161
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:15:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,223
            Genes (coding)                    :: 2,215
            CDSs (with protein)               :: 2,215
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151468_length_1001_cov_3.189218"
                     /isolate="AMR_MDS_0529"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLV77_11270"
     CDS             complement(<1..358)
                     /locus_tag="PLV77_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC16474.1"
                     /translation="MSDEEATEVQPYERSSAEVAELIAAGARLIDVRTAHEHEAARLA
                     GSERIGLEELLDRRDEIEQDGTVIFYCRVGNRSGMAAQAFSEAGFDVVNLTGGIVRWI
                     EDGQPIEPADGYVAEPG"
     gene            complement(493..702)
                     /locus_tag="PLV77_11275"
     CDS             complement(493..702)
                     /locus_tag="PLV77_11275"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HNC16475.1"
                     /translation="MAVVKIIELVGSSRVSTDDAAQQALKQAQDSLRNIRAVDIVSTG
                     IRGENLDEYRAHVRVAFLIEGKDVT"
     gene            complement(781..984)
                     /locus_tag="PLV77_11280"
     CDS             complement(781..984)
                     /locus_tag="PLV77_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC16476.1"
                     /translation="MSEITISQTTTPQEAAAIAAAIQRFSQDTAIAPATEPAGMNPWL
                     KAALEDGVSAKEAFGPGQPFGTL"
BASE COUNT          164 a          326 c          322 g          189 t
ORIGIN      
        1 cacctggttc ggccacgtag ccgtccgccg gctcgatcgg ctggccgtcc tcgatccacc
       61 ggacgatgcc gccggtcagg ttcaccacgt caaagcctgc ttcggagaat gcctgggccg
      121 ccatgccgga acggttaccg acccggcagt agaagatgac cgttccatcc tgctcgatct
      181 catcgcggcg gtcgagcagc tcctcgagcc cgatccgctc ggaaccggcc aggcgcgcag
      241 cctcgtgctc atgcgcggtc cgcacatcga tcaaacgcgc accagccgca atcagttccg
      301 ccacctcggc agaactccgc tcgtaaggct ggacttcggt tgcttcttcg tcactcatgg
      361 gtccggaaag gtagcaggga ggacaggcct ggcccacccc aggcagagcc cctcccaacc
      421 atcaattact tcaagtggga ggggcgggtg tcaactggag ggcgggtcgg accccgcccg
      481 actcctgttc tcctaagtta cgtccttgcc ttcgatcagg aacgcgacgc ggacgtgggc
      541 gcggtactcg tcgaggtttt ccccgcggat gccggtcgag acgatgtcca cagcgcggat
      601 gttgcgaagc gaatcctggg cctgcttgag cgcctgctgg gctgcgtcat cggttgaaac
      661 ccgtgagctg ccaaccagct cgatgatctt cactaccgcc atgaactcct cctcggtcga
      721 tttgactcga tgctagttgc tcgcgcgaag aagtgcgagg gggcggagca gcgggactcg
      781 tcagagcgtg ccgaagggct ggccggggcc gaaagcttcc ttggccgaga ccccgtcttc
      841 gagggcggcc ttcagccagg gattcatccc ggccggttcg gtcgcggggg cgatcgcggt
      901 gtcctgcgaa aagcgctgaa tcgccgcggc gatcgcggcg gcttcctgcg gggtcgtggt
      961 ctggctgatc gtgatttcgc tcatgcggcg tcagattcta t
//
